LOCUS       DAZKOU010000001        76081 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000001 DAZKOU010000000
VERSION     DAZKOU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.001"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..716
                     /locus_tag="ACG827_000001"
     CDS             84..716
                     /locus_tag="ACG827_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482178.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(850..975)
                     /locus_tag="ACG827_000002"
     CDS             complement(850..975)
                     /locus_tag="ACG827_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0482179.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1109..2002)
                     /locus_tag="ACG827_000003"
     CDS             complement(1109..2002)
                     /locus_tag="ACG827_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0482180.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2184..3110)
                     /locus_tag="ACG827_000004"
     CDS             complement(2184..3110)
                     /locus_tag="ACG827_000004"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0482181.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3195..3956)
                     /gene="pflA"
                     /locus_tag="ACG827_000005"
     CDS             complement(3195..3956)
                     /gene="pflA"
                     /locus_tag="ACG827_000005"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0482182.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4200..6431)
                     /gene="pflB"
                     /locus_tag="ACG827_000006"
     CDS             complement(4200..6431)
                     /gene="pflB"
                     /locus_tag="ACG827_000006"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0482183.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6708..9158)
                     /gene="parC"
                     /locus_tag="ACG827_000007"
     CDS             complement(6708..9158)
                     /gene="parC"
                     /locus_tag="ACG827_000007"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0482184.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9170..11215)
                     /gene="parE"
                     /locus_tag="ACG827_000008"
     CDS             complement(9170..11215)
                     /gene="parE"
                     /locus_tag="ACG827_000008"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0482185.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11381..11815)
                     /locus_tag="ACG827_000009"
     CDS             complement(11381..11815)
                     /locus_tag="ACG827_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0482186.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11942..12580
                     /gene="plsY"
                     /locus_tag="ACG827_000010"
     CDS             11942..12580
                     /gene="plsY"
                     /locus_tag="ACG827_000010"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0482187.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12712..13587)
                     /locus_tag="ACG827_000011"
     CDS             complement(12712..13587)
                     /locus_tag="ACG827_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0482188.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13669..14460)
                     /gene="codY"
                     /locus_tag="ACG827_000012"
     CDS             complement(13669..14460)
                     /gene="codY"
                     /locus_tag="ACG827_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0482189.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14478..15878)
                     /gene="hslU"
                     /locus_tag="ACG827_000013"
     CDS             complement(14478..15878)
                     /gene="hslU"
                     /locus_tag="ACG827_000013"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0482190.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15892..16440)
                     /gene="hslV"
                     /locus_tag="ACG827_000014"
     CDS             complement(15892..16440)
                     /gene="hslV"
                     /locus_tag="ACG827_000014"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0482191.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16477..17382)
                     /gene="xerC"
                     /locus_tag="ACG827_000015"
     CDS             complement(16477..17382)
                     /gene="xerC"
                     /locus_tag="ACG827_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0482192.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17580..18956)
                     /gene="trmFO"
                     /locus_tag="ACG827_000016"
     CDS             complement(17580..18956)
                     /gene="trmFO"
                     /locus_tag="ACG827_000016"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0482193.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18925..21003)
                     /gene="topA"
                     /locus_tag="ACG827_000017"
     CDS             complement(18925..21003)
                     /gene="topA"
                     /locus_tag="ACG827_000017"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0482194.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21125..21982)
                     /gene="dprA"
                     /locus_tag="ACG827_000018"
     CDS             complement(21125..21982)
                     /gene="dprA"
                     /locus_tag="ACG827_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0482195.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(22038..22805)
                     /locus_tag="ACG827_000019"
     CDS             complement(22038..22805)
                     /locus_tag="ACG827_000019"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0482196.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22798..23658)
                     /gene="ylqF"
                     /locus_tag="ACG827_000020"
     CDS             complement(22798..23658)
                     /gene="ylqF"
                     /locus_tag="ACG827_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0482197.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23918..24316
                     /locus_tag="ACG827_000021"
     CDS             23918..24316
                     /locus_tag="ACG827_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0482198.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24553..25095
                     /locus_tag="ACG827_000022"
     CDS             24553..25095
                     /locus_tag="ACG827_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0482199.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25178..25729)
                     /gene="lepB"
                     /locus_tag="ACG827_000023"
     CDS             complement(25178..25729)
                     /gene="lepB"
                     /locus_tag="ACG827_000023"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0482200.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25881..26108)
                     /locus_tag="ACG827_000024"
     CDS             complement(25881..26108)
                     /locus_tag="ACG827_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0482201.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26105..26623)
                     /gene="msrA"
                     /locus_tag="ACG827_000025"
     CDS             complement(26105..26623)
                     /gene="msrA"
                     /locus_tag="ACG827_000025"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0482202.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26643..27356)
                     /locus_tag="ACG827_000026"
     CDS             complement(26643..27356)
                     /locus_tag="ACG827_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0482203.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27359..28255)
                     /locus_tag="ACG827_000027"
     CDS             complement(27359..28255)
                     /locus_tag="ACG827_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0482204.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28413..29255)
                     /locus_tag="ACG827_000028"
     CDS             complement(28413..29255)
                     /locus_tag="ACG827_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0482205.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29387..30040
                     /locus_tag="ACG827_000029"
     CDS             29387..30040
                     /locus_tag="ACG827_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0482206.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30103..30630)
                     /locus_tag="ACG827_000030"
     CDS             complement(30103..30630)
                     /locus_tag="ACG827_000030"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0482207.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30650..31597)
                     /locus_tag="ACG827_000031"
     CDS             complement(30650..31597)
                     /locus_tag="ACG827_000031"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0482208.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31609..33492)
                     /locus_tag="ACG827_000032"
     CDS             complement(31609..33492)
                     /locus_tag="ACG827_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0482209.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(33655..35733)
                     /locus_tag="ACG827_000033"
     CDS             complement(33655..35733)
                     /locus_tag="ACG827_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0482210.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35746..36021)
                     /locus_tag="ACG827_000034"
     CDS             complement(35746..36021)
                     /locus_tag="ACG827_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482211.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36383..36838)
                     /locus_tag="ACG827_000035"
     CDS             complement(36383..36838)
                     /locus_tag="ACG827_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482212.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36992..38200)
                     /locus_tag="ACG827_000036"
     CDS             complement(36992..38200)
                     /locus_tag="ACG827_000036"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0482213.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38312..38644)
                     /locus_tag="ACG827_000037"
     CDS             complement(38312..38644)
                     /locus_tag="ACG827_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0482214.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38792..39667
                     /locus_tag="ACG827_000038"
     CDS             38792..39667
                     /locus_tag="ACG827_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0482215.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39749..40330)
                     /locus_tag="ACG827_000039"
     CDS             complement(39749..40330)
                     /locus_tag="ACG827_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0482216.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40336..41595)
                     /locus_tag="ACG827_000040"
     CDS             complement(40336..41595)
                     /locus_tag="ACG827_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0482217.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41597..42643)
                     /locus_tag="ACG827_000041"
     CDS             complement(41597..42643)
                     /locus_tag="ACG827_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482218.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42878..43153)
                     /locus_tag="ACG827_000042"
     CDS             complement(42878..43153)
                     /locus_tag="ACG827_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0482219.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43396..44706)
                     /gene="der"
                     /locus_tag="ACG827_000043"
     CDS             complement(43396..44706)
                     /gene="der"
                     /locus_tag="ACG827_000043"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0482220.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44888..46117)
                     /gene="rpsA"
                     /locus_tag="ACG827_000044"
     CDS             complement(44888..46117)
                     /gene="rpsA"
                     /locus_tag="ACG827_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0482221.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46252..46932)
                     /gene="cmk"
                     /locus_tag="ACG827_000045"
     CDS             complement(46252..46932)
                     /gene="cmk"
                     /locus_tag="ACG827_000045"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0482222.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46981..47616)
                     /locus_tag="ACG827_000046"
     CDS             complement(46981..47616)
                     /locus_tag="ACG827_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0482223.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47682..49085)
                     /locus_tag="ACG827_000047"
     CDS             complement(47682..49085)
                     /locus_tag="ACG827_000047"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0482224.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(49072..50103)
                     /locus_tag="ACG827_000048"
     CDS             complement(49072..50103)
                     /locus_tag="ACG827_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0482225.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50134..50355
                     /locus_tag="ACG827_000049"
     CDS             50134..50355
                     /locus_tag="ACG827_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0482226.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50520..51257)
                     /locus_tag="ACG827_000050"
     CDS             complement(50520..51257)
                     /locus_tag="ACG827_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0482227.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51410..52750)
                     /locus_tag="ACG827_000051"
     CDS             complement(51410..52750)
                     /locus_tag="ACG827_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0482228.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52987..54510)
                     /locus_tag="ACG827_000052"
     CDS             complement(52987..54510)
                     /locus_tag="ACG827_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0482229.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54888..55499)
                     /locus_tag="ACG827_000053"
     CDS             complement(54888..55499)
                     /locus_tag="ACG827_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0482230.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(55622..55747)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55829..56545)
                     /locus_tag="ACG827_000054"
     CDS             complement(55829..56545)
                     /locus_tag="ACG827_000054"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0482231.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(56551..57120)
                     /gene="scpB"
                     /locus_tag="ACG827_000055"
     CDS             complement(56551..57120)
                     /gene="scpB"
                     /locus_tag="ACG827_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0482232.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(57104..57874)
                     /locus_tag="ACG827_000056"
     CDS             complement(57104..57874)
                     /locus_tag="ACG827_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0482233.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57871..58242)
                     /locus_tag="ACG827_000057"
     CDS             complement(57871..58242)
                     /locus_tag="ACG827_000057"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0482234.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(58261..59148)
                     /gene="xerD"
                     /locus_tag="ACG827_000058"
     CDS             complement(58261..59148)
                     /gene="xerD"
                     /locus_tag="ACG827_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0482235.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(59244..59717)
                     /locus_tag="ACG827_000059"
     CDS             complement(59244..59717)
                     /locus_tag="ACG827_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0482236.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59822..60685)
                     /locus_tag="ACG827_000060"
     CDS             complement(59822..60685)
                     /locus_tag="ACG827_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0482237.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(60881..62980)
                     /locus_tag="ACG827_000061"
     CDS             complement(60881..62980)
                     /locus_tag="ACG827_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0482238.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(63200..64309)
                     /gene="rpoD"
                     /locus_tag="ACG827_000062"
     CDS             complement(63200..64309)
                     /gene="rpoD"
                     /locus_tag="ACG827_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0482239.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(64333..66174)
                     /gene="dnaG"
                     /locus_tag="ACG827_000063"
     CDS             complement(64333..66174)
                     /gene="dnaG"
                     /locus_tag="ACG827_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0482240.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(66356..68671)
                     /locus_tag="ACG827_000064"
     CDS             complement(66356..68671)
                     /locus_tag="ACG827_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0482241.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(68856..69716)
                     /locus_tag="ACG827_000065"
     CDS             complement(68856..69716)
                     /locus_tag="ACG827_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0482242.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(69775..70407)
                     /locus_tag="ACG827_000066"
                     /pseudo
     CDS             complement(69775..70407)
                     /locus_tag="ACG827_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            70574..71335
                     /locus_tag="ACG827_000067"
     CDS             70574..71335
                     /locus_tag="ACG827_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0482243.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            71361..73952
                     /gene="mprF"
                     /locus_tag="ACG827_000068"
     CDS             71361..73952
                     /gene="mprF"
                     /locus_tag="ACG827_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0482244.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(74140..75327)
                     /gene="tuf"
                     /locus_tag="ACG827_000069"
     CDS             complement(74140..75327)
                     /gene="tuf"
                     /locus_tag="ACG827_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0482245.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            75695..76051
                     /locus_tag="ACG827_000070"
     CDS             75695..76051
                     /locus_tag="ACG827_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0482246.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        20775 a        16029 c        13054 g        26223 t
ORIGIN      
        1 ttattttgat taaaaaacat cgacatatac tcatcaattt gttattatac tttatatact
       61 tatcaattta atttggagat attatggaaa ttcaacgtag aaccactaat tttttacatt
      121 taggctcttt ttttgcaact ttgacgatct tattttttct atgttattca taccttaaga
      181 cctcacatgc gccttctagt gcattaggct ggttgctttt ggatcattct gatatcacag
      241 aagccgaaga aaatccgttt tttttgattt tgtcttctct ttgcggctta ctgttttctg
      301 tttatttcgg tgcctatttt tttcatcgac atcctggtac gttaaacgaa gcttcaagcg
      361 tattgattgc atttaaaacg ttttcttttt tagcaattct tttttattcg tttaatcaca
      421 atacgctttt cttctttatc atgaatggtt tagtcgtgat tttatccatt tttacttatt
      481 ttattttttc actcattttt agccaattga aatgtccgct cttctttcga ttagttcctg
      541 tcacctgttt tatttacagc ttgttttcct tttttgtcct tgctttaatg ccactgacca
      601 acccttcgac gatccaagtc gcaggaaatc tgttggatat gctcttcatc gtcagtctag
      661 gtgtatttat gtggcaaaga aaaaacaaac aaaataaaaa gcattttgaa gcctgactat
      721 tacgacttca taaatgcttt tttagtttta aacacttcaa tctaaaagaa taacccaaga
      781 ttggataaat tattaatgac tcgccattcg tttcttagtg attctggatt atcaaatgga
      841 cttcctaatc taatcagcta gttcttccca taaaatgcca tacttgactg aatcataata
      901 ttgattattt aaaaatctaa cttttcttat cactacttcc tttgtcattc cacatttctc
      961 accatttttt tgcattcctt tgtttccaga ccatgtagtg aatctaatag gtggtagata
     1021 ggcaaaatac tcaaataatt cttttaacca taaaggaaga gaaaggctcc tcctgcaaga
     1081 gtcagtataa tttttgctta ctgctctctt aggttttact cactaaaact gttccatatt
     1141 ctttgatata agaaaatgca tgcggtataa atttcttgct cagctcagaa aagatttgtc
     1201 gtttaagaaa ggctatttcc tccattgtca atgaaggatc caatgttggt aattcaatct
     1261 caaaattgtt tttttcgaca ataggtaatt ctaatgtgaa tgattcttct tcctttatag
     1321 tattcgtcac aagtagagac tgatggaaaa tactaggccg gcgattttta tatttattct
     1381 tacgtatcct gtcaacgata acttctattt gatcaaaaaa gtacgattca tttacgtctc
     1441 caataagttc ggctaattct ttgaatgcct tcgttacagg aagctgattc ttctgtacaa
     1501 aaaagtaatt agccaaagta agcgggaagt gttctataga ggaaagtgtg ataaaacggt
     1561 acttctcatt tcctgcagtt gtcctcagca attctgtctc tcggctgttt cttactccat
     1621 aaaaatacgc ttctttccgt tcatcttcga aacaatacgc ccaaataact tctcccatcg
     1681 ctaatttttg ttcatcaaca gagagtctct tcaattgttc agctatggtt tcagcagccg
     1741 aagtggcttg ttgcaagtaa tctttaggat caaatattgg aaagttcaaa tgatatcttc
     1801 gttcttccct cttgattaat cctgcatcga tcaataaatc aagaaaatgg gaaaatttat
     1861 tttgcgaaaa ttcttttttc aattctctta gaatcgtttc ttgttctcga tgttcatata
     1921 gataatcggt caatgaagaa aaaagcggat catcggtcag tttttgataa aagttttcct
     1981 tattttttga ataatagttc atatatgttc tcttttctat ccgttttatt ttacttaaaa
     2041 ggcttttacg taaaaaggag gaagaacaag agtcataatt gactgttatt ctcctccatt
     2101 tcttcggcct ttagtatgtt ctatgtttct ttttatatca atcgcttatc cattcatttt
     2161 gaatgcttca tactaccatt tgattagcta aatacagccg tcaattgagg tacgacttgt
     2221 tttttacgtg aaacgacacc tttaagcaaa gcacggttgt tggtcaaagt tgtttggaaa
     2281 gcttcttcta ctttttcttt tcctttacca gcaacaagta attctgaatc actgtctaag
     2341 atgtttgtga caactaatac gaacagatcg tagccatttg ctttcatttc atcttccatt
     2401 gctgcttcta gctctgcttg tcgatccatc acttcattaa ggtccactgt attgacttga
     2461 gcgatacgaa cgttggaact gcccattggg aaactttttg catccaaatc aagaagaacc
     2521 gcagctgatt tatcacttag atttgttcct gctttcaaca tttcaagtcc ataagtgttt
     2581 agatcgactt cagcgattgc tgcaagttct tttgcagctg cttcatcttc aggcgtacat
     2641 gttggtgatt tgaacaacaa tgtatctgaa atgatagctg agagcatgat tcctgcgatt
     2701 tctttaggaa ttgttacttg gttttcttta tatagtttta aaataatcgt acttgtacat
     2761 ccaactggtt ctgcacgata gtacaatgga ttagctgttt caaagtttgc aatacgatgg
     2821 tggtctacta cagctaaaat atttacttcg ctgatatctg aaatactttg ttggaattca
     2881 ttatgatcca caagcatgac atctgcattt tctgctttag ctgactctac tactcgtggt
     2941 gcagatagct gaaagtggtc taatgcgtat tgtgtttctt cattaggtgt acctagagca
     3001 actgcttctg tttctttacc taattcattt tgcaagtgtg aaaacgcaat ggctgccccg
     3061 attgcatctg tatctggatt ttggtgacca aaaactaaaa tttttgacat gtcattcgct
     3121 cctttgtgat ttctcctatt taataatagc aaaaaaacgg aaagaagcaa tacttatctt
     3181 ctttccgatg cgacctatcg cgttaaatat tttgtatact cttctgtatg aagcaatcgt
     3241 ttagcatttt cgacacgttc ttttgtaggt ggttcgatcc cttctaatgg atatttcaaa
     3301 ccaagtgttt cccacttgta ttttcccatc gtatgataag gaagaatctc tactctgtct
     3361 acatttgaca aggtttgaat aaagttgtct agtcggatca aatattcatc ataatcactt
     3421 cgctcaggaa ctagtacatg gcgaatccaa accggtttgt tgatttttga caaatattga
     3481 gccatttcta aaatattttc attgtcccaa tgcgttagtt ttttatgctc ttcattatcg
     3541 atgtgtttga tgtcaaataa cagtaaatct gtatacttca tcaattcttg aaaacgtgaa
     3601 aagaaaggtt cttcatacgt aaaaggtttt ccgcatgtat ccaatgtagt atgcatcttc
     3661 cgctcttttg cttttttaaa tagttcgatc aaaaaatcga tctgcaatag aggttctcca
     3721 ccactaacag taataccacc tttatctccc cagtaaggtc tgaagcgttc tgctttatct
     3781 aaaagttcat ctgctgtgta ttctttccct ccgccaatat tccaagtatc tggattgtga
     3841 caaaattggc aacgcatgcg gcatccttgc ataaacacga caaagcgcag tccaggtccg
     3901 tctactgaac cgaaagtttc gatagaatga acatagccga ttgttttttc ttccatttaa
     3961 gttcacatct tttctttgat ttcattttaa gtacaagtta gctgtcgaag cgaagctttc
     4021 tgaacaaaat ttttcgcaat tgccgcttcg ttatgtgtaa aatttacagg gacggaacat
     4081 acaagtcctg cccctgtaaa tcgtttcttt caaataagca tactgattat tcgattttga
     4141 tgcgatggct attcaacgtc ctttcgacgc taagtcgaaa aacttttttc acgaactttt
     4201 tacattctgt cgtgtgaagt tctcatcaag acatctaatt gttgttctct tgtcaagtca
     4261 cggaatttaa ctgcataacc agatacccgg atcgtcaagt tcggatattt cattggattt
     4321 tccattgcat cacgcaataa atcattactg aatacattga cattcaaatg ataagctcct
     4381 tttgagaagt atccatctaa gacattacgc aagttttcga ttcttgtatc ttgttctttt
     4441 ccaagacctg aaggattgat tgtttgggta tttgaaattc catccaatgc acagccgtaa
     4501 ttcattttag ctgttgagtt caggctagct aataacccac tttgttccgc attgtaactt
     4561 ggatttgctc ctggcgcaag tggttttcca gcttcacggc catcaggagt atttcctgta
     4621 tttttaccgt aaacaacatt tgaagtaatc gtcaataatg aagttgttgg ttttgagttg
     4681 cggtaagttt tatgacgttt gatttgtccc atgaagtatt ccaagatcca tgaaactaat
     4741 tcatctgcac ggtcatcatc atttccgtat ttagggaagt cgccatccac ttcaaaatca
     4801 actgctattc ctttttcatt acggattgct tttacttttg catatttgat cgctgacaag
     4861 ctgtctgccg catgagagat cccagcaata cctgtagcaa atgtgcgttg caagtcagaa
     4921 tccatcaaag ccagctgagc tgcttcataa gaatatttat catgcatata atgaatgatg
     4981 ttcaacgtat tgacatatag ttctgctaac caatccatca tattcatgaa gttatgtttc
     5041 acttcttcaa agtctagtat ttctgatgtg attggacgat attctggtcc aacttgggcg
     5101 ccagtcactt catcgactcc gccattgatg gcatataata gtgttttagc caagttagca
     5161 cgagcgccaa agaattgcat gtcttttccg acaactgttc ctgatacaca gcaagcaatc
     5221 gcacaatcat cagaacccca ttgttcacga agctgattat cattttcgaa ttgaatcgaa
     5281 gaactttcga tagcgattct gctagcatat gttctgaatc cttctggaag gtgttcagaa
     5341 tacaaaacag tcaagtttgg ttctggtgaa ggtcccatat ttgttaatgt atgaagcaga
     5401 cggaaatcat tctttgtaac taatgaacga ccatctaatc ccatacctgc aatggataaa
     5461 gtagcccaga tagggaatcc tgagaataat tggttatatt caggtgtacg cgcgaatttg
     5521 accatacgta atttcattac taagtggtcg atcaattctt gtgcttcttt ttctgtgatt
     5581 actccattat ccaaatcacg ttggatatag atatcaagga atgctgagat acgtccgatt
     5641 gacatcgcag caccattttg tgatttgatt gctgctaaat atccgaagta tagccattgg
     5701 acagcttctt ttgcatttgc agctggttta gaaatatcat agccatattt cgcagccatt
     5761 tctttcattt gcgctaatgc acgatattgt tcagtgattt cttcacgtaa acgaatcact
     5821 tcttcactca ttgtatgata accagtcata tcatgatctt ttttcttttg ttccatcaaa
     5881 tagtcgattc catacaatgc cacacgacga tagtcaccga tgatacgtcc gcgaccgtaa
     5941 gcgtctggaa gccctgtaat gattttgttt ttacgagcag cacgcatttc agcagtataa
     6001 gcgtcaaata caccttgatt atgtgttttg cgccagtcag tgaaaatatg tgtcaattca
     6061 tcgtttggtg tatagccatt gctttttagc gcattgttag ccatattgat tccgccgaac
     6121 ggcatgaatg cttgtttcaa aggtttgtct gtctgtagac cgacgatttt ttccaaatct
     6181 ttatccaaat agcctgcatc gtgtgaagta atagttgata cgatttctgt gtccatatca
     6241 tagacaccgt tacgctcgaa ttggatatca tttaactttt gcaattcatc ccataatttt
     6301 tctgttgctt ctgttgggcc ttccaaaaag ctgtcatcac cgcgatattc agtgtagtta
     6361 ttttgaataa agtctctagt gtctacgcct tctttccatt tggtaccttt aaatccattc
     6421 cattgctcca ttgtatggac ctcctaaatt tctgttttat gtaacagttt ttacgtgatt
     6481 agtataacac agcaccctat ctttgcaagg gttttcttgt tttttattta acaattttta
     6541 tgttttattt acatatattt aaatcaacgt tgacatacca acatttaagc attgtgaaca
     6601 ctttcatctt tttttgcaaa cgtataagga tttattaaaa atgaatgaaa aagagacgcc
     6661 acctgtatta atacagatgg cgtctcttca ttaaaatact acaacagtta cgactcgatg
     6721 aaaatctcag gagcatcgat aacttcgatg acttctccac cttttttctc atccatcgca
     6781 aatgatccat tcgatgttcg atcacctata gggaaatcag ttgggtcaat cgttaactgc
     6841 tgcccttttt gattcatcaa ggttatttct tttgatcttc cttccgacat atagataata
     6901 cggtgcggat tttttttcag ctcacgcatg accgtcactc cccgcttcgc acgtgaagtt
     6961 tggctgattt cttgagcaag catgcgttta acagctcctc gctgagtaac gattacgatc
     7021 ggcgtatcac cttctgctaa cacaagcagc ccgttgataa tcctatctcc atctttcaaa
     7081 ttcatagact tcactccagc tgcctttgct ccaacaacgg gaacatctgt taaaggatag
     7141 cgcaaaccca ttccttgatg cgttactaag aaaacatcta agcctggctg atcttctttc
     7201 agatacacag caatcagttc atcttcttcc gcctttagct tcatacagct gagcggacgg
     7261 ctcttatagg tgcgccatgg ttcgaactct gtcattcgtg tttgcttgat caatccattc
     7321 ttgctgatga atacaaatgt tttctctgct tgaatttttt gatatgggaa aacagctaaa
     7381 atcgattcgt ctacagacag attggagatt gtttgagaaa tgtgctcacc agcatctttc
     7441 catttcaaat cgggtagttc atggacaggg cggtagatca catttgcttt atttgtgatc
     7501 agaagaacat ggtccaatgt actcaattca cctgagtata gcaagtagtc gccttctcgc
     7561 atcccgattt cttctggttt ggaagcacta taagaacgaa ggcttgttcg cttcacatag
     7621 ccttctcgcg tcacactgac aattacgtct tcttgtgcca ctaagacttg cgtatcaatt
     7681 ttgatttctt cgatttcgtc ttcgattgtc gttagacgag cggaagaata attttttttc
     7741 acttctcgca attctttttt cataacagag aaaagctctt tgtcatttga aaggatttta
     7801 ttcaactctg tgatttgcgc aatcaagctt tcggattcct tttgaagctc tgtgatatcc
     7861 gtgttcgtca aacggtataa ttgcaatgtg acaattgctt ctgcttgttc ttctgtaaat
     7921 tgaaagacgt cgactaaatt ctttttggcg tcttttttgt ctttactttc acgaatcgtt
     7981 gcgattactt catccagaat cgataaagct ttcatcaatc cttctacgat atgctgtctt
     8041 ttttgagctt tggctaaatc gaattgacta cgttttgtta tgacttgctt tcgatgctcg
     8101 atataacttc ttaaaatgtc tttcagtcca acttgatgcg gagtcatatg gtcgatagcg
     8161 accatattga aattgtaatt gatctgtagc tcagtgtttt tgaacaaata attcaaaatt
     8221 ccttgagcat tggcgtcttt tttcaattcg acgacaattt gcagacctgt tctatcactt
     8281 tcatctcgta cttcagctat tccatcgatt ttcttattca aacggatctc atccattttt
     8341 ttcaccaagg tcgctttatt tacttcatat gggatttctg tgatcacgat ttgctgttta
     8401 ttacccttca ttgcctctat ctttgttttc gaacgaagaa taacttttcc tcgtcctgtt
     8461 tcataagctt ttttgatttc atcttttcct tgcaagatcc ctcctgttgg aaagtcaggt
     8521 ccaggaatat attccatcaa tttttcaagg ctcgcttgtg gatgatcgat catatagatt
     8581 gtgccatcga tcacttcagc taagttatga gtcgggattt ctgttgcata tccagcagat
     8641 atacctgtcg atccattgac gagaaggttt gggtatcgag ctggaaggac tgttggttct
     8701 ttttctgtat catcaaaatt ccacacgaga tcgactgttt ctttctcgat atctgctagc
     8761 atctctccac ttaacttgga taatctggct tcagtataac gcatcgcagc aggtggatca
     8821 ccgtccatac tcccgttatt cccgtgcatt tcgatcagta cttcccgcaa cttccagtct
     8881 tggctcatgc ggaccatcgc ttcataaata ctgatgtcac catgaggatg ataattcccc
     8941 atgatatttc cgacagattt tgctgattta cggaatcctt tgtcatacgt attgccatct
     9001 ttgttcatgg caaataatat ccggcgttgc actggcttca atccatcacg gatatcaggc
     9061 agtgcacgtt cttggataat atatttggaa taacgtccga agcggtcacc catcacttct
     9121 tctaaagtta attcttgtac ttcttgtcga ttttccatca ggtgacctcc tattctactt
     9181 caaataactg attgttttca tttttatctg ctcgttcttc atctaataaa tcattagaaa
     9241 ctgaaggaga gatttcagta tcctcttttt tatctaagat actgccatct tcttcgagcg
     9301 taaattgcac gtgattttcg atccattttc ttcgaggttc tactttgtca cccatcagtg
     9361 tggttactcg acgttcagct tgcgcagcat catcgatacg gacgcggatc aacgtacggg
     9421 aagtcggatc catcgttgtt tcccacaatt gttccgcatt catctctcca agtcctttat
     9481 atcgctgcag catatatcct ttgccgactt ttttaatcat tgctgccaat tcgtcatctg
     9541 tccacgcata ttcaatgact tgcttctttc cttgtccttt ggatacttta tataaaggcg
     9601 gtaacgcgat atatactttc ccagcttcaa tcagcggttt catataacga taaaagaaag
     9661 taagcagtaa tacttgaata tgggcgccgt ctgtatccgc atcggtcatg atgattactt
     9721 tgtcgtaatt acagtcttcg atagaaaact caggaccgac tcctgcacca atcgtataga
     9781 tcatcgtatt gatttcttca tttttcaaga tatcttgcat tttagctttt tcggtattga
     9841 tgacttttcc tcgtaaaggt agaattgctt ggaactttct gtctcttcct tgtttagcag
     9901 atccgccagc agaatcccct tcgactaaat acaattcatt tttcttaggg tttcgggatt
     9961 gtgcgggtgt caattttcct gaaagaagag attcgccttt tttacgcttc ttcccatttc
    10021 ggctttcttc tctggcttta cgtgctgctt ctctcgcttc tctggctttg attgctttac
    10081 ggaccaacat ctggctcatt tcgctatttt cctgcaaata aaagcccatt tgttcactga
    10141 tgacgttatc tactacggta cgagcaactg gcgtaccaag tttttctttt gtttgccctt
    10201 caaattgcag aagattttca ggtacacgga tagaaagtac agcagctaac ccttctctga
    10261 aatcactacc ttccagattc ttgtcccgtt ctttcagcaa accaactttt ctcgcatatt
    10321 cattataaga tttcgtcatc gccgctttca taccagcttc atgggttccc ccatcttttg
    10381 tccgaacgtt attgacaaaa gataaaacat tttctgaata accgtcattg tattgataag
    10441 cgacctctac ttcgatacct tctttttctc ctgaaaaata aacaactggt gtcaatgtat
    10501 ctttttcttc attcagataa tcaacaaatt ctttgattcc ttcttcgtaa tgaaaaattt
    10561 ccttgactgg ttcttctcca cgaagatcag aaagagagat tttaacgcct tttagaagaa
    10621 aggcagattc tcttaaccgt tcggctaaaa tttcatatga aaatttcgtt gtactgaata
    10681 ttgtatcatc tggtaaaaaa tggacagtcg tcccgtttgc tttttttgtt tttcctatct
    10741 ttttcaaagt accgtcaggt ttaccgccgt ttttaaattt ctgctgatac tctacgccat
    10801 ctctaacgat cgttacaatt agccatttcg ataaggcatt taccacactg gctcctacac
    10861 cgtgcagtcc accagaagtt ttgtaccctc cttgaccaaa ctttccgcca gcatgcagca
    10921 cagtaaaaat cacttcaact gttggtattc cggaagcatg cattcctaca ggcatcccac
    10981 gtccgctatc tgcaacagtt atactattgt cttcatggat ggtcacatca atctcactgc
    11041 cataacctga taatgcttcg tcaactgcat tatcgacgat ttcatagact aaatgatgca
    11101 agcctcggct gtcagtggat ccaatgtaca tccctggtct ttttctaact gcctctaatc
    11161 cttccaatac ttggatggac gagtcattgt attcgttgtt tacttttttt gccaaggcaa
    11221 acgctccttc aataatactt tgcggcaaaa ccgcaccacg attatcatac tagaaaaggt
    11281 cgtgaaaaaa aagagggtag acgagaaatc aattttaatt atctcctaac cgctttttcg
    11341 acattcccct ctatcgatca tctcttcttt gtggtcattt ttatttttgg ttttctttca
    11401 acttaccaag ttcaatctta atacaacgat ccattacgat atctgtacgt cctgcttttc
    11461 ttagcatctc agctgcttct ttgttttcaa tccctaattg tgcccagaac actttagcat
    11521 cagttttcaa aaaatcacct gctatttcag gtaaaaattc actccgtcta aatacatcta
    11581 cgatatcgat tggatcatcg atttctgtca aattcgcata gactttctcg cctagaattt
    11641 cttcaccagc caataatgga tttactggta tgattttata tccattgtct tgcaatacct
    11701 gtgcaaccat gtaacttgtt cgttctggtt tattgctcaa gcctacgacg gcaatatttt
    11761 ttgcttcttc caaatagttg aaaatcgttt cttgtgttgg attttttaca gacatttctg
    11821 ccaccccttg cttttctttt tattccatct attatacttg tatctttcat tttttcaatt
    11881 gttcctgtta cctattcatg ctaaaataaa ggatagtttt caacttgaaa gagggtaaaa
    11941 aatgaaattg atcttattac ttattttagc atatctttta ggttctattc cttccggtgt
    12001 ctggattggc aaattgtttt tcaaaaaaga tatccgacaa cacgggagcg gtaatactgg
    12061 aacgaccaat acatttcgtg tattaggcaa aacagccggg attgttgtcc tgcttatgga
    12121 tattttaaaa ggtacattgg ctacttgttt gccgatgatc ttccaccttt ctgggattaa
    12181 ccctctttgg tttggggtat gcgccattct tggccataca tttccgattt ttgctgggtt
    12241 caaaggagga aaagctgttg cgacaagtgc aggaatgctt ttaggattta atcctgtatt
    12301 ttttgtttat tcttgtatca tctttatcgt tagtttattt tgtacaagta tggtgagttt
    12361 gagcagtatg atcagtgcag tattgatcac tttatctacg atcgtccttc cttatgtagc
    12421 tcctgtgatt ttggctaagc ccaattggtt actgacgatc attgcctttt tagtttcttc
    12481 ctttatcttt tatcggcata aagacaatat caagcgtatc agaaaccata ctgaaagtcg
    12541 aatcccgttt ggtttgaata gcagcaagaa aaaatcctga tactttttca ttccaaagag
    12601 ataaaacggg agtgtgacaa atcgatgtca cactccctta ttacttacag gatagctttt
    12661 ttacacacct tggtaaacgg tgttccctgc taacttctcg gtcacaacct cttacttcac
    12721 tgtgatggaa tattttgtct tgaagatttc atgggcacct agttgattga tccctacttt
    12781 ttccatcaag tttccactac tgttcaccgt gtcagcgatt ccgcaccacg gttcgataca
    12841 aacgaaaggc gcttcttttg gataaggaga ccagatccct acataaggca tatctttata
    12901 gcttaaagtt acgctatggg aagatttttc acttcgaatg gcgtacgcat ttagacccct
    12961 tgtttcaaaa accaaggcat cttgctcaaa aagttcacga ttcaaagcca gattcgtatt
    13021 tgtctgacct aacgtctttt gatccatatc gatgaatggg cctttcaatg gtaggcgtaa
    13081 tcttgatttc aatggagaaa atgccagata gaaatcttca aatgacaatc cttcttcaag
    13141 aggtacattg aaagcaggat gtccaccgat agaaaagaac atttcttttt caccggtatt
    13201 tgccacttga taatgaaccg tgatgccgtc tcctcctaat tcatagctga tcactaattc
    13261 aaaatcaaat ggatactttt ctaatgtctc cggcgtactc cttaagatga aactagccat
    13321 ttcttgtcca tgttcaatga cttcaaaatc catatcccga gcaaatccgt gttgccccat
    13381 ttgatatgtt ttcccttgat aaacatactg atcatctttt aaacgtccaa caaaagggaa
    13441 taaaaccggt gcatgtctgc cccaatacgc tggatcacct tgccagatat actctaaacc
    13501 atgttctttt gatttcagac tgatcaattc cgcaccgtgt tcagcgattt cggcgatcag
    13561 ctggtcattt tgaataataa cagtcattgc cattcctcca acttcgactt actttttaaa
    13621 atgggaaggc tggaacaaac atttctatct gccccagtcc ccttacttct aataactgtg
    13681 ttttgctaac tcgtctttaa aacgggaatt caatactttt aaataagttc ccttcatgcc
    13741 taacgaacga gattcaatga ttccagctga ttccaatttt cgtaaagcat tgacgatcac
    13801 agaacgagtg atgccgatac tatcggcaat attagaagca gtcagacgac cttcatctcc
    13861 atccaatgct tcaaagatag cttgtacagc cttcaattcg ctataagata atgtgttgat
    13921 tgccatttgg acagccgttg cactccgtac atctgcttcg atgcttcgag acttctgata
    13981 aaggatctgc ataccgacaa ccgtagctcc atattctgct aagactagat cgtcatctgc
    14041 aaacgattgt tccatacgag ataagataat cgtacccaaa cgttcgccag ctccaaagat
    14101 cggaacgaca gtcgtcagac caaatggata tttctctctt gactctaccg gaaatgcagt
    14161 aatatcacta gtaattggga tattagcttc agttttcatc aactgtgcca ctgtatcagt
    14221 ataagattca ggaaattgtt tctcaacaaa cattgttttg attcgttcat tgttcacatc
    14281 gtgacgttca ttataaccta gtaatactcc gtcacttcct atgatatagg tgttgctgtc
    14341 aagaatacca cctagtgtga cagccatttt atcgtaaggc aaatctgctt gcatgtcaaa
    14401 agtattcttt tgctgcaaaa gtgtgtttat cttgcgggtt ttcgttagca agtctgtcat
    14461 tgtcgtcctc cgatttttta taaaatatag cgacttaaat cttcatttgc cgcaattgag
    14521 ccaagtttgt catccacata tgcttctgtg atcgttattt ctcccatttg catatctggt
    14581 gcttcaaata atagatcttc tagtagtttt tccaaaatgg tatgcagtct gcgtgcgccg
    14641 atattatctg tgtcacggtt gacatcgaaa gcgatttgag cgatccgttc aattgcttct
    14701 tttgtaaacg taactgacac attttctgta ccgatcaacg cgacgtattg ttttactaaa
    14761 gcattatttg gttcggtcag aatcttcaca aaatcttcag ctgtcaaatc atctaattct
    14821 acccgtatcg ggaagcgtcc ttgaagttct ggaattagat cacttggttt agacaaatgg
    14881 aaagctcctg aagcaatgaa taggacgtga tctgtttgga tgatcccgta ttttgtgttt
    14941 acttgtgaac cttcgacaat tggcaatatg tctctttgga cgccttctct ggatacttct
    15001 ccagattgct gtgatttaga agtgatctta tcgatttcat cgataaaaat aattccactt
    15061 tcttccgcta atttgattgc ctcactatgg atatcagcat ccttgactaa ttttgctgat
    15121 tcttccttga tcaataactc taaagcttct ttgactgtta ccgtccgttc cacttttttc
    15181 ttcggtgtca aagcacctag agtttcattt aggtcgatcc ccatttgctc caatccattg
    15241 ttcatcattg gtgcaggttt cttttggtct tcaacttcaa tggttacttc acgactatcc
    15301 agtaaaccct tttgtagttg ttccaagact gtacggcgat tgattcgaat atcttctgtc
    15361 acttcttctt gcggttcagc ggtattttgc tgactagcat taaaaagatt catcatttgt
    15421 tcaaattgat tagtggattg tttctgttct tttttgatac caggtaccaa gatttttacc
    15481 aaacgttttt ctgcattttt tttagcactt gtgtatacac ggctgtactg ctctttttct
    15541 acgatttgga tcgcattttc tactaaatcc cgaaccattg attctacatc tcttccgaca
    15601 taaccgactt cagtaaattt tgttgcttcg actttgacaa atggtgcatt gacgattttt
    15661 gccaaacgtc gtgcgatttc tgttttaccg acccctgtag gtccgatcat cagcatattt
    15721 tttggtgtaa tatcttgttg cattttttca tctaactgca aacggcgata gcggttacgc
    15781 aaagcaacgg caacagattt ttttgctgcg tgctgtccta cgatatattc atctaattca
    15841 cttacgattt gtcttggtgt ttgttttaat tcatacatat ctactcacct cttacagttc
    15901 ttctacgata atattatgat tagtaaagat acagatatct gcggcaatat ttaacgcatt
    15961 ttctgcaatt tctttcgcac tcatgtcgtt ttgagcatag tttttcattg ctcgggctgc
    16021 agccaacgca aaattccctc ctgatccgat agctaagata ccatcatctg gtgtgataac
    16081 ttctcctgta cctgaaacaa gaagcatttc tttatcattc attacgatca gcatcgcttc
    16141 aagtttttgc atagattgct gtgtccgcca ttcttgagct aattcgacag ctgcgcgttg
    16201 tagattcccg ttatattcgt ttagtttgcc ttcaaatttt tcttctaaag taaacgcatc
    16261 tgcaacactt ccggcgaaac ctacaacaac ttctccattg tagatacgtc gtactttctt
    16321 tgcagtgcct ttcatgacaa cagattcacc cattgttact tggccatctc cagccatagc
    16381 aaatttcccg tctttttcga cggcacagat agtggttgaa tgaaatggtg attcgaccat
    16441 gtttgtttcc tcctaaattt gttcttttcc ttatttttac gctcttggat ggaaagaacg
    16501 atagtttttt tgtaaacttt cttttgttac atgggcatag atctgggtag tcgataaatt
    16561 ggcatgtcct agcaattctt ggactgtccg caaatctgcc ccattattta ataaatgagt
    16621 agcaaatgta tggcgcagca tgtgaggatg gatatcacta tccaaactgc tttttttgat
    16681 catttgattc aagacatatt caattccagt aggcgtgatt ggttttccat gatgattcac
    16741 aaaaacgata tcatgctctt cgtgatattt tgtcatcaat acctggcggc cttccctgaa
    16801 atattcttgc agagcatctt gagcaaaaga acctagagga gcatatctct ctttgttccc
    16861 tttaccatgg atcaacatga cctctgaatc aaaatcaatg tctgataccg acaaactgct
    16921 acactcactt aaacggatgc ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa
    16981 atccaaagga ctatctcctg ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa
    17041 aaaacgtggc agtctcaaat ttttcttttt gagatgaacg taagaaaatg gattttcttt
    17101 tatcacttct tctttgagaa gatactgata aaacgaacgt aggctggcta tcttgcgact
    17161 gatagaattt ctgctatagc taggacgatg atcagctaat gcactcaaat agacacgaac
    17221 cgtaaaatat tccgccttca gcagatcagc gtctccagat tcctccaaaa aatgtttgaa
    17281 atggagaatg tcttcttcat atgcttcttt cgttttttct gaatatcctc tttcgacaat
    17341 caaataacgc aaaaattttt gttcccacgt ggcttctgtc attttctcac cttccttttg
    17401 tcccagagaa taatttagca cagcgtttat aaaaagacaa ataaattgtc agaatttaac
    17461 caatatttct aagtttgttc acaattggcg caattcctca cattttgttt gagaaattct
    17521 taagaaaaac gcaagaaaaa gagcgagact atccgtctca ccctcttcat ggagtttcct
    17581 tactcatttg ggattgcagt tttttctaat tctgttttta ttgcctcatt agcttttaaa
    17641 gctcgttgtg caattgcttc atatcttgct tttttgtctc gaatgcgctc tggcagttca
    17701 ggaaacagac catagttggc attcatcggt tggaaatgtt ttccttccgc atgagtaatg
    17761 tagtaagcca tacttccaat agctgtttct tgtggaaact cgactggttc catttctttt
    17821 gctaagcgtg cggcgttgat tccagcaagt agtccgctcg ctgcactttc aacatagcct
    17881 tctacaccag tcatctgacc tgcaaagaac agatcttcgc gtttcgttga ttggtaagtc
    17941 ggtttcagca attctggcga attcataaag ctgtttcggt gcatcacacc atagcgaacg
    18001 aattcagcat tttctaaccc aggaatcatc cggaaaacac gtttttgctc gccccatttc
    18061 aagtgagtct gaaagcccac gatgttgtat aaagaagctg cagcattgtc ttgacgcagc
    18121 tggataacag cataaggacg tttgccagtt ttaggatctt ccaatccaac tggtttcata
    18181 ggtccgaaaa gcatcgtttt gaatcctcgc tgtgccatta cttcaattgg catacatcct
    18241 tcaaaatatt tttctttctc aaaagattta agttctgcca cttcggcatt gattagtgcc
    18301 tcatggaaag ctttgaattc ctcttctgtc atcggacagt tcaagtaagc tgcttctcct
    18361 ttgtcatagc gggacttcaa atatacttta tccatatcga tcgtagcttt gtctacgatt
    18421 ggtgcagctg catcatagaa ataaaaacca tctgaaccat tgaatgcttt gatgctttct
    18481 gctaatggat gagaagtcaa aggtcctgtt gctactacgg tgattccttc cggtatttca
    18541 gtaatttctt cgtttatcac tgttactaat ggatgctctt tgacttttcg ggtaattgtt
    18601 tcagaaaaag aatcgcggtc aacagctaag gcaccaccag ctggcactgc tgtttcatct
    18661 gccgaagtga ttactaccga attcaatcgg cgcatttctt ctttcaataa ccctactgca
    18721 ttagccaaac tgtttcctct taatgaattg gaacatacta gttcagcaaa attacttgtg
    18781 tgatgcgcct cagtcgattt gactggacgc atttcatata aacggacagg gactcctgct
    18841 tgtgcgatct gccaagcagc ttcacttccg gcaagccctg ctccaacaac atttaccatt
    18901 ttcaatgaaa gaacctcttt tctattattt ttggatgtct tcttcgtaat ctccatttac
    18961 acagactact tgtttaccgc ctttgacttt cttctcgacc agatatccgt cacattttgg
    19021 acaatttctg cccactggtt tatcccaaga agtgaattca cattctggga aacgggaaca
    19081 accatagaat agtctgtttt tcttagattt tcgttcaatg acttgtcctt catgacatac
    19141 tgggcaagtc acaccgattt cttttacgat tggtttcgta ttccgacaat caggaaaatt
    19201 actgcaagca taaaacttcc catatttccc taattttatc accatcggat gtccacagac
    19261 atcacaatcg aatcctgctg gttcatcttt gatttgaatt ttttcgattt tttcttccgc
    19321 attcgtcagt tctttttcaa acggtttata gaaacgatca accactttga cccacgcttc
    19381 cgtgccttct tcaattttat ccaaatctcc ttccatttct gctgtgaaat ggatatcgac
    19441 gatttgaggg aagaattcac agattaatga attgacaatt tctcctagtt cagtcggctc
    19501 aaatcttttt tgtgtcaatt ttacatagta gcgtctttgg atcgtatcaa gtgttggtgc
    19561 ataagtggaa ggtcgcccta ccccgttttc ttctagtgca cgaatcaacg tagcctcact
    19621 aaaacgtgct ggtggttgag taaaatgttg ttttggttca atatcaacag attggactac
    19681 atcgccttct tcaagttctg gaagaatatt ttctttctct tctttgccgt catctctacc
    19741 ttccacatac acttgcatga atcctttgaa tttgattttt gaaccgttcg caatgaagat
    19801 gacaccattt tgttctaatg tcactttcat tgtatctaaa atagctggtg tcatctgact
    19861 agccacaaaa cgtgaccaga tcaatgtata aagtttcaat tgatctttat ctaaatattg
    19921 tttgagttca tctggtgtac gaagcacact cgttggacgc acagcttcat gggcatcttg
    19981 tgcgccttga gcatttttga tctttcggtg atcatgtcta gaaaattctt ttccatacgt
    20041 atcttcaata aatgctgcgg cctctgcttt tgctgtatcg gcaatccggg tagaatcagt
    20101 acgcatgtat gtaatcaagc cgactgttcc ttgtttaccc aaagtgattc cttcatataa
    20161 ttgctgtgct accatcatgg tttttcttgt tcgaaagttt aacttacggg cagcttcttg
    20221 ttgcaggcta cttgtagtga aaggattcgc agggtttctt ttgcgctctt tcttttctac
    20281 ttttttaact tcgaagtctg ttccatcaat tctagcagtg acttctttta cactttgtgc
    20341 atctggtaat ttctttttct taccgtcgat tccccagaaa tttgctttga atttcttgcg
    20401 agctttttta aaattaccgt cgatgctcca atattcttct ggaacaaatt ctcggatctc
    20461 tttttcacgg tcaataataa tttttaaagc gacagattgg acacgtcctg cacttaaccc
    20521 tttcttgacc ttacgccaga gaataggact gatcgaataa ccaacgattc tgtctaaggt
    20581 acgacgagct tgctgtgcat ctactaaatc tacatcgatc gttcttggtt ccttaaaagc
    20641 tgctttgact gcgtcttttg tgatttcatt gaagaccacc cgattttttt ctttcagatc
    20701 caacccaagt aggtacgcta aatgccaagc aatcgcttct ccttctctat ccgggtcact
    20761 tgccaagtaa actttttgag ctttttttgc tgcggctttc aggcttttga tgacatcgcc
    20821 ttttccgcgt atagaaatat aatggggttc ataattgttt tcaaaatcga tacccatttt
    20881 acttttcggt aaatctctta tatggccaac actggccatt actttatagt ttcgtccaag
    20941 atatttctca attgtcttgg cttttgcagg tgattctaca atcactaaat atttatacgc
    21001 cattactgaa tggactcctt tccattgtcg tgttcctact attaaaagag gattcataca
    21061 aatcgtagct aatcatatcc attcagaaaa cgtttgtcaa gaaaagcaaa tgaaaagtat
    21121 ggtgttattc tttaaattct gccaagatat cttcacccga aaccgtgcaa actgcaccgt
    21181 cttggatcaa atgatgacat ccggtagaat gttctgttaa aatatttcct ggaacagaaa
    21241 aaactgttct tccaagatcc aatgcttgct gtgctgttat cagagagccg ctttttttct
    21301 tggcttcaaa gacacatgtt cctttagaca aagctgcgat aatccgattt ctcattggaa
    21361 aacaatgctt tttcgggccg gattgaggag gatattcact caaaagaaga tgatcatttg
    21421 caatcacctc ttgcaagtca cgattttcag caggatagac ttgctctatc cctgttccaa
    21481 tgactgcgat tgtccgtcca ccatgagaga ttgctgcttg gtgaccgtat gtatctatcc
    21541 ctttcgcgca gccactgacg atcacatatc cagcttctat cactggtttt agtaaataat
    21601 aaactgtttt tttggctaaa gcagaagtgt gtcttccacc aacaacgctt aacatgttac
    21661 aagaaagtaa agatagatca ccattataaa acagtacaat tggcggatag gtcaagtgaa
    21721 gcagctgagg gggatatttg ggatcaaagc aagtgataaa cgactgtttc gattggattt
    21781 tgccccagtt agcgtgaatt tcctgccaac ttttgcgaaa cagctcccgg tgtttggtta
    21841 ttttacctat cttaatgatg tcttcaaatc cgatttcttt ataaggatac tgcatcatcc
    21901 agttagcaag tgcccatttt ccttgactac tgattccttt tgtataagca agtttcaata
    21961 aattttcttc tatttgatac atcataaaac cgcctttcgt ctctctttct aataagatac
    22021 gtaaaagcgg ctgtttttta ttgtaaatat ttttttacag gtgcaaaact tttacgatgg
    22081 attggcgtaa ttccttgtcg ttcaagtcct tctaagtgtt cctttgttcc ataacctgca
    22141 ttatgcgaga aaccatatcc aggataaagc acgtcatatt ctttcatcaa ttggtctcga
    22201 tatactttag caatgatact tgcagcagca atcgacacag atcgtgcatc ccctttgatt
    22261 attttttcct gctgaatagg caaatccaag gtcatcgcat cgatcaacaa atagcttggt
    22321 acaatggaca atttttctac tgcttggatc atcgctaatt tacttgcttc ataaatattc
    22381 acatcatcga ttgtctgctg atcaacgatc ccgatcccga ttgccagggc tttttcttga
    22441 acttgttgat aaagaagttc tcgctttttc tctgataatt gtttggagtc attcagccct
    22501 aatatgatct catttttcgg caagataact gcagctgcca cgacaggtcc agctaatggt
    22561 cctcttccta cttcatcaat gccggcaatc cattggtgac cttgtgcgta cccttgcttt
    22621 tcaaagcaaa acatttcttc atatttttcc ttaattaatg cttccttttt caatcgtcgt
    22681 tcaaaagaag ccagtgcttg ctgcacgcct ttgcgtggat catttcttaa tgattctagt
    22741 gtaggatcat ctattctctg tatgcttttc aatcgttctt tgatactttg gatggattca
    22801 gtcatcactt ttttgctcca tttcttcgca acgatccagt gtgtatcgtc caagttttcc
    22861 ttgacgaata tcatgcacga ctaattcgct gccacgatca tagtcttctt tatacccacg
    22921 ttttgctgta atatccatca gtaattccgg tgccaatttt tcttcttgct cgattgataa
    22981 accataacga tcgctgattc ttcctgaata atatcgggca aaaaagtcta atccgtaaat
    23041 cgctaaatca tctaaatgga caagctggtc tttgatcgcg cctgtcagcg ctagtttttt
    23101 gccgatttct tcatcttcaa atttgggcca taaaatccct ggtgtgtcta acaattccaa
    23161 ctcattacct aaacgaagcc actgctgacc ttttgttacg ccaggcttat ttcctgtctg
    23221 tgcaattttc ttgcctacta gtcgattcat caacgtagat ttcccaacat ttggaatacc
    23281 taagaccatt gcccgaatag cgcgaggctt gatcccccga gcagcatctc ttgcccgttt
    23341 ttctttcaat gcttctttag ccgcaggtac tagaccttta attcctttac tttcttgtgc
    23401 cgtgatagct aatgcaagat ggccttgttt acgaaaatat tcttgccaca gctttgtctg
    23461 ttgtggatct gccagatccg ctttattcaa taaaacaagg cgaggttttt gctgaatgat
    23521 ctgatctagc attggattac gagaagataa aggtaatcgt gcgtctacca attcaaatac
    23581 gatgtccaca tatttcattt tttcagaaac ttctcgtcgg gcttttgcca tatgtcccgg
    23641 aaaccattgg atggtcattg ttctctccat tcctttcata ccggaatatt ccccggcttt
    23701 acttgttttt ttctttttca atataaattc acttttttat tataaagaaa ttccatctat
    23761 tagttaagtg aaagtgaacc agtcctcatt ctatcacatc ttcacttttt tgatgatgca
    23821 aatttttatt ttggtttcta tatatgaatc taaatttttg acagcgtttc ctaaataaga
    23881 gatgatagac gaacaaaaca gaaaggagac gaaacaaatg aatagtatgg tttttgttaa
    23941 ttttcctgtt gctgatgtca atcgttccgt tgctttttat gaaaagctgg gttttaaaaa
    24001 aaatgaagaa ttttctactg aagaagctgg ttcaatggta tgggatgaca ctttttggat
    24061 aatgcttctc tctcatgatt tttataaaaa atttataaaa gataaagaaa tagctgataa
    24121 taaaaaaaca agtggtgctt tgatttcgtt tagtgttgaa aatgcggatg aagtaaaaaa
    24181 atttgctcaa attgcaaaag aaaatggtgg tactttccac gaagtagata tgggaatgcc
    24241 tgaagaagaa atgttttcat tagaagtcca agatctagac ggaaatacat tagaacccgt
    24301 atggatgaag atgtaagcag ataactaatc tgacgttttt tataacaaaa aaacaaaggc
    24361 ggatgatacg ataagtattc tccgcctcta ttttttttat tcgtacaatc tcttttttac
    24421 agtttataaa agcagtcttc tcaccctttt tttaattgtt agatgccagt gtatgtaaat
    24481 caccaatgtg tcatgacaca ttggtgattt tatggtatcc ttttattatt aactgacaaa
    24541 ggagagaata ttttgaacac tttgattgca aaagacgttt ttagattgcc cgaaatgaat
    24601 gcaagattgt tgtcgacctc tgctctttta ttagcccttg atgtcatctt atacaagata
    24661 gcgattggtc ctgcttattt tcaaatcaca ttcggatttt taagtatggc gcttatgggt
    24721 tatctatatg gaccgatctg ggctggtttg cttgaaatgt tgtcgaacac tttaaatttc
    24781 acgatatttg gttcaggaac gtttcaagct gcttttctta tcacggcttt ccttggtgga
    24841 tttattaatg gcttattttt atatcataaa aagataaacc tgccttccgt catcgctgcc
    24901 caattattga ttatgatccc agtcagcctt tttatcaatt cttggctgat gtctgttttg
    24961 tttggtgcag atttcaaagt aatttttcta ggtagattca tcaggaatat gatccaaatc
    25021 cctattcaaa taatggttgt ttattcattt atcaaaactt tagaggctag aaaagtgttt
    25081 aaacgagctg tttgatttta cgtactaagg tagtaaactg attttggatt aaacaataaa
    25141 gcgtgcagtc tcaacagact gcacgcttta ttgtttctca ctggatccat tttatttcat
    25201 caagtggata atagacaaat cttgctttac ctagaatatc agttttattg atcgtgccaa
    25261 acgaacgact atcttttgaa actcttcgat tatctccaag tacaaaataa ttatcttccg
    25321 gaagcttgga ttgtcccgtt agctcttgta gtgtaaagtc cgtcgtatag gatgcatttg
    25381 catgatctga tttcaggttt ttagttagat aaggttcctt aactactttc ccattgatct
    25441 tcaattgatc attttggtag ctgacatttt ctcctggaag accgattact cgtttgatat
    25501 aaatcgtgcc atctgctaat tgaaaaacaa caatatcaaa tcgtctgatc tccgaaaatt
    25561 tttccatcac aaccatatca ccttgcttca acacgttttc cattgaattg ccttccactg
    25621 gtaccgggat gaataaaaat ccccgcaaca tgaacgcaag gaatacagaa acaagtaagt
    25681 atttgaatac aagccagaag cgattaatat agcgttgttt ttttgtcaat ctctatcaat
    25741 ccatccctct ttttttcatt ctttctttat tataagaaaa atatcaaaaa aatgcgacta
    25801 gatagctgaa ataaaaataa ttctttcaat taggctattg gacaaaaaac ttttttgatc
    25861 ataaagtaca aaactatctc ttatatgtca tgattatttt ctaaatattt ttcccatgct
    25921 tcgtcaaaga tatccatgga cggcaaatag tcgacattca gttccagata attagaaatt
    25981 tcttcgtagc tagagctgtg cttaggaaat tggatgtctt taccggcttc tgtagcgaaa
    26041 acagataaag catcatgtgt gccaccgcct cgcagtgtca ttaaataatg aaaaaagctt
    26101 cgattcaccc ttcgttcctc caattttctt cgataaaggc agcccggcgt gcatggtttc
    26161 ttttaaaatt ttcaggatgc tttttataat actcttgatg gtcttcttct gctagataaa
    26221 atggttgggc tggttcgatt tttgtaacga ttggctgttc gaaacgtcca ctttcagcta
    26281 actgtttctt acttttttca gcaatgatcc gctgttcttc tgtcaaataa aagatcaccg
    26341 ggcgataatt gtcaccgcga tcctcgaatt gtccgaaagc gtcagttgga tcagtttgct
    26401 gccagtaaag ttctactaat tgttcatatg aaatcaatgt tggatcatag ataatctcca
    26461 ctgcttctgt atgtccagtc gtttggcttt ttacttgttc ataagtcgga tctggtacat
    26521 gccctccagt atatcctgac atcactgtat ggataccagg aagcgtgtcg aaaggctgga
    26581 tcatacacca gaagcagcct cctgcaaata ttgctcgttc cattatgatt ccccctctaa
    26641 tactactcat ccgatgattt tttattttct tttgtcttgc tttcagtact gctggtgcta
    26701 gttttgttgg tatttgagtt gtttgaagca tcagaggtat cttttggcaa atatagacta
    26761 aaccgaatgt catcatttac taagtcgatt ttatctgctt taatatacat cccgttttgc
    26821 attcggaatt gatccaacct aagcatgata gtcagatcat ctgtattgac ttcgatccaa
    26881 tctggtaatt tatagtttct cttgatcata ttcatcacat ctttgattgg caaactcaat
    26941 gttccgatag aaagacttct agcttttagt tggacatttc cgttttccat cacatacgga
    27001 tcaaaatata aatagaaatc gataggaaaa cctagtactt cgaaagtacc attgagtagt
    27061 gcttcatttt ccaaataaaa tttatattca atatcactat ctttttgata ctcactaagg
    27121 aaaaagctga tgatttgatt gactttttct ttatttgaat tgatcgttaa gacaggatct
    27181 ccttgtcttt ccgtaagagc tggtatttct gggagttctg gttcccgatt agcaaaaata
    27241 cggctgccta aaaatacggt cgaaccaagt actaccccaa gaaggataag gaaggcgaat
    27301 ttccagtaat ttgttttctt tgttttgttt gaagatacgt cagatcttgt tttcatgttt
    27361 attgatcatc ctccttctct aaccattggt tttgtgtctc gatcagctga tctctcactg
    27421 catttgccat caattgataa cctaaattat ttggatggaa atgatcttct tcatagagca
    27481 cattattttt gatgccattt agattttctg tcgtgctagt tgtttgatct gttccttctt
    27541 ctgtgacacc tacttcctga ttcaatcctt gataaagcaa atcattgatt gggataaagt
    27601 agcagttcgg catttttttt gtcaccgttt ctgttccttt gttccaattg ttaacaattg
    27661 tctgcatgtc agtgatctca ggaaagttca aataaaatgg attgtagatg cctaaaatgt
    27721 aaataggtgc ggttttattg agggatcgaa attcttccaa tagttctgtg acattttcct
    27781 gatagttttt cagtggcttt ttaaaggaat tgaccgttaa cccgaataaa ttattttgga
    27841 taactttcat caaatcatta ccgccaacag tcagtgtgat aaaatcagct gttttgatat
    27901 tgtttcgaat ttcttctttt gtttttactc gctttaagat ctggtcactt cgttcaccat
    27961 ttacaccata gttttctgtt tcaaccgcgg tcaaattata acggtctttt agatcatttg
    28021 cgacaagggg aacgaatcct ccttgcttgg tttgatctcc tactccttca gtcagcgaat
    28081 ctccgatagc tatgtagtga agtttttctt ttttatttgg atcttctctc acagtagtta
    28141 ctggtttcat tagtgggcca gctttcggta tagaagcagc taaacctgca tagatgatga
    28201 tcgcaattaa taatgggaac aaaacgagga tcaaatgtcg tttcgttaat ttcatttttt
    28261 cacctcttgt attcgattgg ttctaatgtt atcaaaaatt ttatcaaaaa ggtagtcttt
    28321 atgaaaaaag aaaacaatta ttttcctaat tttataaaga aaaagagaat aagctgcaag
    28381 cctttcaggc tgagggctta tcctcttttc gactaatcta cataatacat gattgcaaac
    28441 gcgtctttac cagtatgcgt agcgataacc ggattcgtat gcagtacagg gatatgcatg
    28501 tccgggaata attcttgcaa accctctttg aattcttgcg ccaattcttt tgcatccgca
    28561 tgtgaaatac cgatttgtcg cacatttggc aattctttca gttcattttt caaattatta
    28621 aaccatttat cgaacgtttt ctttcctcgc cccttagtta cagggattaa ttcagaatgg
    28681 gcgaaattca tcaccacgcg tatgttcaaa acactagaca gcaaaccagt tgttcggctg
    28741 attcttccac cttttaccaa gttatctagt gtggatattc cgatgtataa ttttgtattg
    28801 tcttgtactt ttttgacttc ttcaaggatt ttctctactg ttttaccttc ttttgctaat
    28861 tcagctgctc gaattacctg aaaagataat ccttggtcag tggtatcact gtcgataacc
    28921 gttactttcg tttttgttag attactggct tgtcttgctg cttctactgt tccactcaag
    28981 ccttttgtca tatggatcga aatcacttca ctgccgtctt ctcctagctc atcatacatc
    29041 gagatgaatt ctccaattgg tggttggctt gttttgggca gattttttgc tttcgccatc
    29101 atttccataa atctttcgcc gtctagttgg tcatcatcgg gatatactgt tccgtcgatc
    29161 atgatggata gcggcatgac acgaatgcct aaagcatcgc gtacattttt ttccatcgta
    29221 catgaagaat ctgttacgat ttttacattt gtcattattt tctctctctc tttctttaac
    29281 aagatgcctt attcatgtta aactacatgt aagacaattt ctatttttag tataacagac
    29341 aaataatgat ttttcattaa ttacacctag taaaggagcc gttttcatgg aaaaaacgaa
    29401 atttaatcga acgtatgaaa tattaaatga agtgtttaat gcagtcaccc atggaattgg
    29461 gactggattg agtatcgcgg gactagtggt tcttttagta aaaggtgccc acttgcattc
    29521 ctccctccat attgtctctt atgctatcta tggatcgatg atgattcttt tatttttaac
    29581 gtctactttg tttcattcat tgatttttac aaaagcaaaa aaggtgttcc aagtattcga
    29641 tcatagttcg atttttcttt tgattgcagg aagttatact cctttttgtt tgctgagtat
    29701 ccgaggatgg ctaggttgga ctttatttgt attaatctgg ttgtttgcga ttcttggcat
    29761 tatatataaa gcgttgacgt tgcataaacg ggagagagta tcaaaagtat ctacgatcat
    29821 ctatattttt atggggtggc tctgtgtcgt ggctgctgtt cctctttatc actctcttgg
    29881 aatgactggt gttattttga tggtagcagg cgggctttct tacacgatag gcgcattctt
    29941 ttatagcttg aattctatcc gctatatgca tgttgtttgg catttgtttg ttatgttagg
    30001 tgcaggtttt atgtacttct ctgttttatt ctttacttaa taaaaaaaat aaagctatgg
    30061 atgggacagc tctaaataac agctagtcta tccatagctt ttttatatcg tcaattcgcc
    30121 tgtcccgctt tttacaggat tctcattacg ctgatacgtt tcaaattgat gcggataacg
    30181 atttttttca tccatttctc cttgactagt attgatcagt gaccaatcag accagtcaac
    30241 aggtggaaaa taagcatctc catcgaatac atgatggatg actgtacggt ataatatcgt
    30301 acaaaatggc agaaattctt gataaacagc tgaccctcca gcgatgaaaa agataccgtc
    30361 tgtttctttt tccagagcaa gcacgtcttc cacactatgc atgacatgca cgccttctgc
    30421 tttataggaa gaatctctcg tcaaaacaat cgtctgacga ttcggcaacg gacggctacc
    30481 catcccttcg aatgtttttc gtcccatcac tagtgtattt gcttctgtca tttgtttgaa
    30541 gaatttcaga tcatttggca aacgccaagg aagacggtct tcttttccaa tcaatccgtt
    30601 ttcatcctgt gcccaaattg cagctaacat tttctcatcc tttctgattc taaacagcga
    30661 taggagcttt gatcgccgga tgtgggtcat atccttctaa ttggatatct tccatctcaa
    30721 aatcaaatac tgactgcttt tcctgattca aaactaaagt cggaaatgcg cgtatttctc
    30781 ggctcaattg ttctttcatt tgttcgatat gattgatata aagatgagca tctcctaatg
    30841 tgtgaacaaa ttcgccaacc gctaaacctg tttcatgtgc aattaggtga gtcagcaacg
    30901 cataacttgc aatgttgaac ggaacgccta gaaacacatc cccactgcgt tgatacaatt
    30961 ggcagcttag ccgtccctct tgaacataaa attgaaacat cgtatgacaa ggaggcaaag
    31021 ccatgctagg aacatcttcc ggattccatg cagaaacgat caatcttcta gaatcaggag
    31081 tctttttgat tgcttcgatc acttgtttga tttgatcgat aaagccaccg tcttttgttt
    31141 cccagtgacg ccattgatag ccgtagatgt gtccaagatc gccgtatgtt tcagcaaagg
    31201 aatcatcggt taggatcttc tcgcaaaaaa gcttgctctg ctttttatat tcttcattga
    31261 attcaggatc tgtcaaacat ctatggccaa aatcagtcat atctggtcca ttatagtctg
    31321 gacttttgat gtagcgttca aatgcccatt catcccaaat atggttttta ttttgtaata
    31381 gatagcggat attcgtatct ccttttaaaa accaaagcaa ttcgcttttg atcaatccaa
    31441 aaggtactcg tttcgttgtt aacaaaggaa accctttttg cagatcaaac cgcatttggt
    31501 atccgaataa gcttaatgtg cctgtgcctg ttctgtcttc tttacgatgc ccttcttcca
    31561 acaattttct tcctaagtca agatacgctt gttccatcct ttttcctcct aatcttctac
    31621 ccattcactc aagtattccc accgtgccat tttttcttca agttgtgctt ctgtttcaga
    31681 tacgtcgttt tgcaattctt gtaatcgagt gaaattatct ccttgatgat tcatctcttc
    31741 ttggagcaat gagatttttt cttcaagttc tgcaatctca tcttcaatcg tttcccattc
    31801 tttttgttcc atgtaagata atttcttctt ttcagcaggt tccttgctct gtatttttgg
    31861 tttcgctttt tcagcaggct gctgggtctc tttttgcttc agtaaataac tggacatgtt
    31921 tccgtaataa agttcttgtt ttccgtttcc ttggaatact aataacttat ctgctacctt
    31981 gtccaagaaa taacggtcat gggaaacagt gatcacagca cctggaaaac ttctaaaata
    32041 atcttccaaa atcgtcaatg ttgctatgtc caaatcattg gtcggttcat ccaataataa
    32101 aacattaggt tgctgaatca gcagtttcaa caaaaataac cggcgttttt ctccgcctga
    32161 aagtttgcct atcagtgtcc catgcataaa tcttggaaat aaaaatcgtt caagtaattc
    32221 tgctactccg atttgactgc catctgctgt ttttgcttct tcagcagctt cttgtaagta
    32281 agcaatcatc cgtttatctg gtgccatttc ttcattttct tgtgtataat acgcaagatg
    32341 aaccgtttca ccgatagaca ttgtaccgct atccagggga attctgccag ctagaatatt
    32401 aagcaatgtc gattttcccg caccattttt accagtaata cctagtcttt cccgagattg
    32461 aatcaataaa tctaaatgtt ctagcagtgt ttggtcattt atacggtaac tgccatcttt
    32521 gatttccagt acttttttcc ctaaacgttg tgtcgctagg ttcagctcta aatcatcttc
    32581 ttggtttact tggtacaagt tctcctttag atcttcgaaa cggttgatcc gtgcttgctg
    32641 cttggtgcca cgtgcctgta cgccagccct catccaagct aattcctgtt tataaagccg
    32701 cttgcgcttt tcttcttgtt ctacttctac acgatcacgt tctgcttttt ctaagacgta
    32761 agcttcataa tttcctttat actcgtataa ttttccaaat gaaagttcga agattcggtt
    32821 tgctactcta tctagaaaat aacgatcgtg ggtaaccatc aatagtgcgc cacggtattg
    32881 tttcaaatag ttttccaacc actcgatcgc ttcataatct aaatggtttg ttggttcgtc
    32941 tagtaatagt aaatcgggtt catcaatcaa cacttgcgcc agactaactc gttttttctg
    33001 accacctgat aaagtactga tttttttatc taattcactg attcctaatt tttgcaaaat
    33061 aatctttgcg ttggtatctg tagtccaagc atcttcttta ttcatttgtt cttctgcttt
    33121 agcataacgt ttttgcacat cagggtcttc tccgttttcc cctagttcga tcaatgcttc
    33181 ctcatatgct cgaacagttt ggatcaaggg gctgtttcct tgaaagactg cttggagaac
    33241 agttaattca tcggaaaatt cagctgcttg agaaagataa ccaatacggt aatcagttgg
    33301 ataaaacact gtttgccgat caccgtcgct gctatcgata ccggcaataa tatttaagag
    33361 acttgtttta cccgtcccgt tcgtcccaat caacccgata cggtcttttt cgtggaccaa
    33421 aaaagaaatc tggtcaaaca aatctttttc accataagtt tttttgaggt cagttacttt
    33481 taattctttc aacatgttcc ctccttatct tggatattgt atcataaaaa tagtgaactt
    33541 caggaaaatt tttttggtat ttgttctttt tgtgttctca aaaaaagaac aggaccgatt
    33601 cgatcctgtt ctgatatgtt ttgtttttta taaaacttga aaatttttac ctttttattt
    33661 tattggactc gttttttttg actttgctcg aaacgcaaat cggaaagaaa agttatcaga
    33721 tggtttgttc atcgtataag attctaacaa attgatattc ctgcgatagc tgttcgcaga
    33781 aaacgtcgtg attttttcag caacttcgta ttcatcaatg atttttcttt caagataata
    33841 acctcttagc atttttttcg taaacagact atcttgctgg atcatccacg aagaaatcaa
    33901 attccccgag ttcattttta ttccttcaag tttgcgttgc tggataaaaa attgaaccat
    33961 cgtttcatca taaatatcag tcaagttatc taaactttca ataatcaact gagtattttt
    34021 ttgatatgtc tctctgattt cttggatatc ttcatcacta ataaatgtct gacgatcagc
    34081 atatcttctt tgccaaaaca ttttcggatg gagaacgatt cgcattaacc tacggacaac
    34141 aatgaaccaa aagccgataa aagacaaaat ctggcttgtg atagaatgtt cgaatcgaga
    34201 aagaaaacgg gagtaaaaac gataaccatt agaagaaagc ttgcccgaac gataactttc
    34261 atccaatcca tcccgttcga tcgacaaaat cagtgcttgt atttcttgta cttcttgctt
    34321 ttcatcatcg gttaaatctt ccaagtacag atcacgtatc cgttcttgat aattttctat
    34381 cactgcttca gtggctaaaa attcttttgt attacgatca atttccttta tttcttttcg
    34441 caaaacttca acgacctctt ctaaaatcat caaagcattc aaatccgtgc ttttcgcttc
    34501 ctctgtttcc gtcagcatag gcaagacaat gatccctatg accaaagtca ctaagatgac
    34561 acccgccgtc agaaacaaaa gcaaagaccg ctggtaaaac atcatattat tgattgaaaa
    34621 tggcaaaata aaaatcgtag ccaaactgac tgttcccttt acaccgccaa atgttaagat
    34681 caagatctcg ttcaactgtt ttttgaactt tttactgcca tctttgaata cataaaaaag
    34741 actgatagaa atgaatctga tcacaaaaag catgacagag attaatacga taatcgctaa
    34801 taaaagaccg tttggatata aaccgtctcc ccatactggc gagaacactt gcgtcaattc
    34861 gattcctaaa aatataaata ctaatgcatt cagtgtaaaa gcaatagttg tccatgtact
    34921 gtgggacaag ctcgacaatt ctgcatcaaa cacagagatt ttcctaaaac cagaagcttg
    34981 gagaatacct gctgccactg cagcaatgat tccagataca tcaaatgcct ctgccaatac
    35041 ataggctaga aaaggaagca aaagttcgat cagcagatac cctgtgacat cttgtgctga
    35101 tgctttttcg atcaaatgaa ttattttttg tttgaaccag acaagcaaaa agccgatcaa
    35161 agctccccca atactagaaa ctactagact catccccgcg tttactgctg aaaaagatcc
    35221 ggtgatcagc gcaccaagag caaactggaa ggcagtaaca ccagatgcat cattcaaaag
    35281 accttctcct tccaaaatat gcatcgcctt tttagggata ttcactcgtc cagaaagaga
    35341 actgactgct accgcatctg tcggacctaa cgctgctcca aaagcaaagc aggcagcaag
    35401 tgggacactt ggcaacagga atgataacgt cgcacctacc ccgacaaggg tcaaaatgac
    35461 tcctccaaat gccaagaaca aaattgtccc gaaatttttg agaatcgaag aaatatctgc
    35521 tttttctcct tctctaaata acaatggtgc aataatcata actaagaaca attcaggttc
    35581 aaaatcgatt gattgtcccc actctgtcag acctaatacc actccaagga aaatttggat
    35641 cagtggagct ggaatcattg gcaaaatgcg gctagctatg ttcgataagg tgatcgttac
    35701 tgcaaataca ataactaact cagcaaattc catcgtccat cccctttatt ttttattttg
    35761 tgtaagcatt tttaaaaatt gttctttttt ctttaaaatt ttggtttcga ctacttcaac
    35821 ctgtgtttgg ttcccctcta attcacactg tacacgtttt gactccaaac gaagcagatc
    35881 atgtttcaga cgttccaact gacgatacat caatatattt cttttacgca acttacaaag
    35941 tatttgacgg cggtcatcca tcgaaagggc gccatagcga tcatgcaaca gttttttttg
    36001 ttgttcaaaa ctctctctca ttttccagat ctcctataca caaacttcca tcattatagc
    36061 aaagttcttt ttggatgaaa aatatttcat tgtttcatcg tttattgcta gttagaaatt
    36121 tccataaatt tttcaacgcc tcattctatt ccaaatagta tcagatatca agtaaaactt
    36181 aagaaagata aaagtttctg acaaaaggat gtttactttc ccattcctgc ctttcttgtt
    36241 cttttatcac aacaaaaaaa tggggctgcg acaaaaatct tgtcacagtc ccttattctg
    36301 aataaacggt gtggaagaga cagcctatca gtagtaataa gacacatctc tttcacagcc
    36361 tcatttttgc gtaaaaatcg atttattcga aaaattcccc gtatacatct tctacacgtt
    36421 gatctggatt gatcacgata tacaagttac ctgttttttc gctgattttt gttgattgat
    36481 attgattgtc taatccttct gttcctttat ctagatatgg gaagtacaca agatcaggtg
    36541 tgccatttcg gtaaaggatt tccattcgct cgatatcttc gtatttcaat gcgcgattaa
    36601 acatgcccat ttccagtccg cctaggttga tatcatgtgt cgcaactgcg tccccttcat
    36661 ggaagatttc gattttaaat ccagcacacg gatggatttc aacaaattca ctgccattga
    36721 ttcgtccaaa acttgttgtg atttgtttga tccaaagatc gccgatatga cggcggttga
    36781 tcgtccaggt ttctccattt ctaaaataaa attttagcgc tgtcatttct ttactcatta
    36841 aaaagactcc tttatgatgt aatttattct tgtactacct cagtttagca caaatttatg
    36901 taagtggtca catcttatgt tttttcttct aatgacgtat ctgcccactt taagtagcca
    36961 acaaagctga gcagatacag tctagtgttt tttaaatcaa tttttctttc gataaaaaat
    37021 ccaaaacctg ttttttatca ttttttattc tttgttctac gacggctttt tcagctaatt
    37081 gcaaagcttc actaacccac ttaccaggtt tttttccagt ttcttctagc aagattttac
    37141 catctactgc taattcgctt aatgacttga taggtaaacg atcataggat tctcctacgg
    37201 catccatctg aggtgtttcg tcaaaataga tcatcaattc ttctacttgc agtgcatgtt
    37261 ttttgcctaa gaagtacaat tcgaatggtt ccaaagctcc agtttctcgt tgtcgtagtc
    37321 cttggacaag tgcttgaacg tctttaatca tttggttaga acacttccac gatttcaaaa
    37381 aggaacgaat ctcattttct tttagatcca gttggctgat caataatgcc catgcttggc
    37441 tctcttcttt caatgggacg gatggtaatt cagcaaaacg taaaagtgct tcgccttctt
    37501 ctttcaaacc gggacagcaa atgtagcact ctgtttcaat aaatgcatgg attcccgcgg
    37561 cccgttcatt tcccaagagc atcttgatga attccacatt tatacgttct acagatattt
    37621 tacctaatag ctcgtggttt tctttgatgg cgtagaaggt ttcttcctct aatttgaacc
    37681 ctagttgact gacaaaacgc aacccgcgca tcattcgtaa ggcatcttca tggaaccgct
    37741 catgaggatt gcctacagct cgcaagatct gttcttctag atcttgcaga ccatcgaaca
    37801 aatcgatgat ttctccatct tctttcaaag caaacgcatt gatcgtaaaa tctcttcttt
    37861 tcaaatcttc tgccaacgaa cgaacgaatt cgacatgatc tggtctacgg tagtcctgat
    37921 aagttgattc cgtccgaaat gtggtgatct catattgttc atcattttct aataccaaca
    37981 cagttccatg ttcgatccca atatctatcg tttttggaaa aagctgtttg atttcttctg
    38041 gaaatgcgct tgttgcgata tccacatcat ggattggctg gttcagcaag atatcacgaa
    38101 cacttccacc tacaaaataa gcttcatatc ctgcattctc taattttttc agaactggaa
    38161 ttgccttttg gtattccaga ggtaattgtt ttaatttcat ttttccaacc tttcctgcgt
    38221 gtttcttgtc ctgagataaa gactcagttg catggtgact gtttgacaag tccgtttcag
    38281 agtcttcaag gccaggacca cttcatcttg tttactcgtc cttacgctca aagcgattat
    38341 ggtctctttg attgaatttt tccatgtttt tttcaaaaac ttcagttaag tcgatattca
    38401 atgaattagc catgatcatc gtaacgaata aaacatcacc aagttcttct gctaccgagt
    38461 taactggttc agaagccttt ttgctttttt ctccataagt gtggttgact tctcttgcca
    38521 attcacctac ttcttcagtt aatcgagcca tctgagctaa tggagagaaa taccccacct
    38581 tgaattgctg gatatattca tccacttcct gctgcatact gtataaagaa cgctttttat
    38641 ccatcttttt ccctccaaaa ctatcttacc aaaactttaa cgcaaatcct agataagagc
    38701 aattgagaaa atcgaatttc catgctacat ttaggaagga atttctaaat ggaaattctg
    38761 tgaaaataca taattgaaag ctggaattca tatggtcaaa cgaaaaacag taaaagaagt
    38821 tctttttatc attataggaa caagtattta tgcatttggt cttgtttact tgaacatcgc
    38881 taatcattta gcagaagggg gagtctcagg gatcaccttg atcttaagag ctttatttgg
    38941 tattgatcca gcttatacga ctttattaat caacatccca ttgattttac taggaggaaa
    39001 gatcctcggt aaacgttcct tggcctatac tgttctaggt acactttctt tgtcattctt
    39061 tttatggatc tggcaaaggg ttccactaga aatcaattta cagcatgatc tactgatcgt
    39121 ttctctactt gcgggtttag ttgcaggtgt aggaagcggg atcgtctacc gcatgggtgg
    39181 aacaacaggc ggcagtgata ttatcgctcg gattttagaa caaaattacg gcatcactat
    39241 ggggcgttcc ctattggcat ttgatatcat cgtcttactt gcttcattaa cctatatcga
    39301 cttgaaacgt atgatgtata cgttgattgt ttcgtatgtt ttcagtcgtg tgattgattc
    39361 gatcttagat ggcggatatt ctgcaaaagg tctacttgtc gtctccaaca aaagcgaaga
    39421 gattgcacca ctactgatga ccggtctgca aagaggtgtg actttccttt ctggagaagg
    39481 cgcattctct ggtaattcaa aaaaaatcat ctatatggtc gttagctcta gtgaattaac
    39541 tgaagctaag aggatcgtcc acgagattga cgaaaaagct ttcctttcaa tcatcaatgt
    39601 gcatgaagtg gaagggcaag gctttactta tttgaagcca cagacaagat tactgaaaaa
    39661 actataagaa aaaagacaaa gataggctat gaggaaagat tccacatcca gctgaccttt
    39721 gtctttttta gtactatact atttttaact aattgttatg ttcacttacc cggttacttt
    39781 tcttttgtgt ctgcaaaagg atctttcggt taacttcatc agataatttc aaaaaagcat
    39841 ccctatctcc atcttctagt gcttgatcga tttgagcata aagcattttt agttttgcct
    39901 cttcttcttt ttcttgaaaa tatcgttcaa ttaattcagt atcttcttca ctgactgaag
    39961 aatcccaaga agaaaatgga ttgtcttcta agatagatag atattctctt gtctgccaag
    40021 cattttcgaa tatacattcc acatataaag gctttttcca attcaaacgg atttcatgaa
    40081 aaatctggtc tgtgtccgta aattcttttc cctgcaaaaa caatttcatg ggttcccctt
    40141 caacagatag atcactgatc tgcagtcctc gatccgcaaa attcgcccct tcaacaaagt
    40201 ggacattgct taaaatcgct tcatgattgg caagataatt caagatccaa aaaacttctc
    40261 ttcggctaaa agatacatga ttgaccatcc acgttaaaaa attcttcttt tcccgtacat
    40321 caattagcat ttttctcacc tctctgttag gtcatctaat aatgacagtg cctccatatc
    40381 tcctggttca agtgttaaat aataagtcaa taattcttgc gctcttttca gctgcccttc
    40441 ttctcttaag aaaaaggcat actctttcaa gaaatctggt tcatgtgata gttcatgata
    40501 agcctgttca tagtgtacag ctgcaacggt aaagtcttct aattcattat aagcatgggc
    40561 taggttccat tctgcataag gattagcagt ttcttccatc tgattgatca cttgtatcac
    40621 atcttcatat ctttcatcat ccaagtaaag attacttaaa gtaagtaatg tttcatcttg
    40681 tttgtctcct acttctaaag ctttcagtaa aagttcttct gctcgctgtc tatcatgcag
    40741 acggaaagca ttttccgagg ccaggtgata aagttcgact tggtaaggat tttctttgat
    40801 tccttcttct aacactgttt gtgcttcttc aatcatttct tcttcttgaa gagcttgtcc
    40861 tagatataga tataacgatt gataatgcgg attaacttct cgaagttctt ccagatagtt
    40921 gattgcctgc tgattttctt tcaattgaag attcgtaaaa gctagctgga acagtcgatc
    40981 atctgtcctt tcttctttca gtgcttcttc taaaggtacg atagctgctt cgaattctcc
    41041 ttgcatactt aaggattgtc ctaaacgttc ctgcatcgag attcctgaaa tttcatttat
    41101 cccgcttgcc aacaatgttt cataaaccac tgctgcctct ttgaaacgat caactgaaaa
    41161 atacaattca gctagcgcaa actggatcaa tggttcatca ggaagcaacc cagctgcttc
    41221 tttcaatttt gcttcactca cttcagggat tccgataact tggtacaaat cagctatcgc
    41281 caataagctt tgcggatagt aatcactttg cttatccact ttttccaggt attcaaaagc
    41341 taagtcgatc tcattatctt cgattgcaat ttctgctaac gggatgttca gtccgtcatt
    41401 ttctgggtat tggttgatca gttgttcaaa aatctgtttt gcttcgatca aaaagccaat
    41461 agccagcaat tcttcgccta gttcagctaa tatatctgct gggtcttctt ttaatgcttt
    41521 ttctaaaaag atttgtgctt gtgacaagtc ttcatctgat aaagcttgca acattttttc
    41581 gctatttgta ttcatttcaa ttccctctta tctcactgac gatttgttga aataccgtca
    41641 ttgttctcaa taattctttt tcgtttggat gttggtatcc gtctattata cctatttttt
    41701 ctaaaacaag tatttttttg cttttttctg ccaaaaaaag aactttttcc aaatactcca
    41761 tctttgaaat ctgttccagc cagtggattg gatagccaag caagtccagc cacacaaaaa
    41821 agttttttag ttggaaatca aaaggatatg cttggagatt ccacacgaaa tgaaacatca
    41881 ttcctaaaaa ttttttcata ctagcagaaa gtagatggcc attttcggtc agataaaaag
    41941 cttgttcaaa cagtttacca taatttgaca ggttttctgc ctctaattga taaaaataga
    42001 tcatttcttc gatcatcccg gcaaacggcc gagtcgttaa ttgcttttta tttggatagt
    42061 ttttatacag attttgcaaa aaacgatgat cacaaatcat tccgcaacag ataaaagtca
    42121 aaaagtcaat ctgctttccg tccacctgcc gctcgctgat tgtttgatca taaacgatgc
    42181 gttctggcag attgatggtt tgcagcacta cacgatttgg tagctgcaaa atcgtccgct
    42241 gcggtgttaa tgctcggctt agagaacgga tcgatacagg gatcatccat agtgatgaat
    42301 caagggttgt atgtttttga aaaaaacctg ccagatccat gatcccttca tttccaaatc
    42361 cgatgataaa catggattga ttttcgggat agcgtttgac gaaatcaagc aggttcttta
    42421 attctgttaa atgattgcat tgagtgttgg cacaaatata ccagtcattg tttgcttgat
    42481 ttgcaaataa cgggttgatc ttatctgcaa acaaatcata atagcgctga ttcgttaaaa
    42541 ataagattgg ctgatcaatg gaaagttctc ttagctgact gactagcgtt tctccgtaaa
    42601 acactttcgt caacgcatct tctacttgat aatcaagttc catacgcatc cctcctcttc
    42661 ttatttttat tttaccatta gtaagcaaat tctagaaaag ataagagcaa agctcacctc
    42721 ataacttgca aataaataaa aattaaataa aatacatgtg ttttctatgt atgacatacg
    42781 gattcggaat ctctgtctct tatttattcc ttttctgcta ggaaataaaa agaaaagcac
    42841 ttgtagccct agagccacaa gcgctttttc aactgtctta tttaacagcg tcttttaatg
    42901 ctttacctgg tttgaatgca ggtactttgc tagctggaat ttcgatttct tcaccagttt
    42961 gtgggttacg acctttgcga gctgcgcgtt cgcgaacttc aaagttacca aaaccgatta
    43021 attgaacttt ttcgccgtta gctaaagcat cttgaatagt tgaaaataca gcatctactg
    43081 ctgcagttgc atctttttta gttaaatcag tagctgaagc aactttttcg attaattctg
    43141 ctttgtttgc catggataga ttcacctcct ccacaagaga tatgatagaa atatcattta
    43201 gttaaaactt tctgagtggt ccagaagttt aaaaagaaat attgattaac ttcaatattc
    43261 tgttatcacg atatcatatg aaccgcttaa tagcaaggtt tttaccaatt ttcacatgct
    43321 ttttttagtc atagaaacgt aacaaacgaa actgtctttt tcttttgaaa aaaaaacgtc
    43381 gatatggtaa aatgcctatt ttctcctacg agggataatt cggatggacg tcccttcaaa
    43441 agtaaatgct tttctgattt gattttctaa gaaacgttca taagaaaaat gcatcaattc
    43501 ctcttcattg acaaaaacga caaatgtagg tggtttgaca gcgacttgtg tagcataaaa
    43561 aatcttcagg cgttttcctt tatctgttgg tgttggattg attgctacag catccataat
    43621 gatatcattc aacacagccg aagagatacg aagattctgg ttcatactta cttgttcgat
    43681 catttcaggc agacgttcca accgttgttt agtcactgca gaaacaaaaa cgattggagc
    43741 gtaatcaaga taccggaatt catcacgaat ctcttgttcg aaatcacgca ttgtattggt
    43801 ttctttcttt acaagatccc atttattcac gacgataata attccacgac cagcttcatg
    43861 ggcgtaaccc gctactcgct tgtcttgttc ccgaatccct tcttcagcat tcagtaccat
    43921 caaaacgata tcagaacggt caatggcacg cattgcacgc atcacgctgt atttttcagt
    43981 attttcgtat acttttccgc gtttacgcat accagctgta tctatcatta agaatttctg
    44041 accactttca gaaacgaagt gggtgtcgat tgcgtctcgg gtcgttcctt caatatccga
    44101 aacaattaca cgttcttctc ctaaaatcgc attgatcaaa gaggatttac caacatttgg
    44161 gcgaccaatc aaactaaact taatcgtgtc gtcgtcctct tcttcagtct cttcagagaa
    44221 atacttcact gcttcatcta aaatatcacc aatccctagt ccatggcttc ctgaaacagg
    44281 ataaggatcg cctagcccta aggaataaaa ttcatagata tcgtttctca tttctggatt
    44341 atctacttta ttgactgcta aaatcactgg tttattactt ctgtataaaa ttttcgcaac
    44401 catctcatct gcgtctgtca cgccttcgcg tccgctgact acacaaatga ttacatctgc
    44461 ttcttcaatc gcgatttctg cttgatgctt gatctgatcc ataaaaggtt catcgccaag
    44521 atcaattccg cctgtgtcga tcacgctgaa ttctcgacct aaccattcac cttttgcata
    44581 aatacgatca cgagttactc caggagtatc ttcgacaatc gagatacgct caccagcgat
    44641 gcggttaaaa atcgtagact taccgacgtt cgggcggcca acgattgcaa ttgttggatt
    44701 tgccatatta tttcctcctc acaagtttcc aatctcactt agtttaacga atgcattgcc
    44761 aactttcaag catagattgt caattaaacg aagtattttt attccttatt gaaatactaa
    44821 tcgcttttca aaagaaaaac tgagacaaaa gcagctgctt tcatctcagt cacagataca
    44881 aaatcaatta tttttcagaa tcatcggttg ctttttcttc tacttcttct ttcaacgcat
    44941 cacctaaaat gtctcccata gtaaatccag tattttcttc tggtaattca taagactcaa
    45001 cattttttgg ttcttcttct gaagctggtt tttcttctaa agctttgata ctcaatgcaa
    45061 tacgatgttc ttctggatgg atctctaata ctttcacttt cacttcgtca ccttcatgaa
    45121 gcacttcatg tggtgtcgca atatgtttat gtgaaatttg agagatatgg actagccctt
    45181 ctactccagg gaacacttca acaaaggcac cgaagcttgt caaacgttta actgttccgt
    45241 ctaatactgt gccaactgga gcttttgtat cgatttctga ccaaggtcca ggctgtgtgt
    45301 ctttgattga caaggaaaca cgttcttgtt caggattgat agacaacact ttgacattta
    45361 catcatcatt gacagccaat acatcacttg gttttgctac atgactatgt gaaatctctg
    45421 agacatggac tagaccgtca attccgccta ggtcgacaaa tgcgccaaaa tcagtaagac
    45481 gtgcaacacg tccactgaga acatctccct catggatagt tgaaagaatt tcttttttct
    45541 tttcttctct ttcgttttcc actaccgctt tatgcgataa gatcaaacga ttttctgatg
    45601 gttcgatttc gatgatttta aagctcaatg ttttgccttt atagtctgaa aagtctgcaa
    45661 caaaatgatc ttccaccatt gatgcaggga caaaaccacg tacaccgacg tcaacgacta
    45721 aaccgccttt gacaacattt gtaacaggtg cttcgatgac cttgccctct tggaaatctc
    45781 tttcgatatc ttcccaaact tttttagcat ctaaacggcg ttttgaaagt agatagctgc
    45841 cgttttcttt atctttgccg atcgtactga taacgactaa atctaataca tcaccgactt
    45901 ttactacttc gctgatatct tctaccggca aagttgataa ttcttttgcc ggaacgactc
    45961 cttcaactcc agccccttcg attccgacaa tcacttgttt gtcttcaatg actaaaactt
    46021 cgcccttaac aacatcgcca acttttactt cttgtacgct tttcattgcg tcttccattg
    46081 actggttgtt ctcttgattt tgatcaaatt ctgtcataac acttgtcctc ctaaccaaca
    46141 ttctgccgta agtacggtac tgtttttagt ttaattgatt attcaaaaaa aataaagcga
    46201 tttcgttcgt tttccttgaa aaaacaattg tttttcattt tttttcttaa tttaaaaaag
    46261 aagataacct tttttcaaaa caacagcttt tattgctgca acgacttctg gaatcgattt
    46321 gccagtcgta tcgatacgaa cagcatcctc tgcttgcttc agcggagaga cgtcccttgt
    46381 tgaatcataa tggtctcttt cctgtatctc tttcgtcagt gtttcaagat cagtcggaat
    46441 tcctttttct tggttctctt tgaaacgtct ttctgcacgc tcacttacgc ttgctactaa
    46501 aaagattttc acttctgcat tcggtaatac agctgtcccg atgtctcttc cgtccatgac
    46561 aacgccaccg cgttccccta tttttcgttg gacagcaact aattcttctc tgacttttcc
    46621 atgtgcagag acttcagaaa cagcttttgt gacatctggt tgacggattt ctaaagtcac
    46681 atcttttgat gctatgaata catgctgtcc atcttcttgt tgcgaaaaag agataggata
    46741 ctttttgatc agcgaaacta gcccttcttc atctgaaaaa gatacattgt tcgtaatagc
    46801 taaataagtt actgcgcgat acatggcacc ggtatctgta taaatataat tcaattcttt
    46861 tgctaatatc ttggcaacgg tacttttccc agatgaagca ggcccgtcga ttgcaatact
    46921 gatttgtgtc atgatataac tcctttttct cagaaaaaaa gccgcaaagc gcggctatta
    46981 ttatttaatt cttaattctt gaccagggta aagcataaag ttgtttggat ctaagccatt
    47041 caattcaaac aattgatcag tggtgatccc tgcacgctca gccacttgtt ttggaccttc
    47101 gcctgcttga acagtcgttg ttgaaccggc tgcagctgat gaagaaggtg ttgtttctgc
    47161 tgcattgctg cttgaatccc ctgcttgata agttgcctca cttgaggaag tcggtgttgt
    47221 ctctgctgca atactgcttt ctgacgttac acctgtatct tgagacacag tagattcttc
    47281 cgcgattgta ctagattcta ctgttgaagt ctcagttgac tcctcttttg ttgtacttgt
    47341 agaactttct actactgatg tcgaagtttc agaagaagtt gctgctgttg cattgttgct
    47401 tgatccattt ctgatccaga aataagcacc tgctggaata gcgatacata agaccagtaa
    47461 aatcaaaaat agcgaaagaa ataatgtatt tcctttcttt tgttgtctct gagaacttct
    47521 tgatgaaact tcatcatctg tatcgtagat tggttgttcc catggttctt gtgtttcgtt
    47581 attttctgta gattgatgtc tatcttttct gctcactgtt ttacctccta aactctatcg
    47641 tgtgtattat accataggaa aaagtgtctt gtcctaaaaa atcaatgttc catgtttttt
    47701 ttaaataaat ttagtaaaat cgttccccaa tgaagttgtt cagtcttaga aaaaagcttc
    47761 ctctttggca gaagttctgc ttttgctcca tcaatatcac aacaattctg cggcttttcg
    47821 gagagttttt cgccaaaata agctaagatg aattttctgc ggcagttttc ttcattaata
    47881 tagcgaagca tctgctgcag gcggaattct ttttcttttt cctgacgttt gagccgttcc
    47941 acccaatttt ctggtttttc agactgctgg atcagctcca tccatttttt ttgcagctca
    48001 tcaaagggag cttgctctgt ggcatattcc aaataaagct caaaactttg tcgctgttct
    48061 ctagaaagtt gattaaagaa ataatggata cgttcatccc cactttgata aaaaagaatt
    48121 gctgcacttt cttcctgatc tctgcctgct cgaccgattt cttgtacata gttttctaga
    48181 ctgtctggca aatcataatg gaccacataa cggatatcag atttatcgat ccccatacca
    48241 aatgcattcg tagcaatgag aaactgcagc tgatttttta caaactgctg ttgaagttgt
    48301 cttctttgag ctgcatcaag accaccgtga tagtatccaa ctgtaaaacg ttccctgaat
    48361 aaatgataca atcgttcgac ttcttttttt gttgcacaat aaatgattgc cgctccttgt
    48421 gctctttcca tgaagtgttc aagttcttgt tctttttgct gcgttttccg aacaaaaagc
    48481 gagatatttt gccgattgac tgattgtctg atctcttttg gtggtcgatc aaatagtaca
    48541 tgacgtatat ctcttgctac taaatctgtt gctgtcgccg ttagtgccag tgtgaccggg
    48601 tttcctaaaa gctttcggat ttttcctaac tgttgatatt ctggacggaa atcaactccc
    48661 cattgagaca cgcaatgagc ctcatctact acatataacg caatattttg ttttttcaga
    48721 tgctctaaaa gagaaggatt agtcaacatt tcgggactca aaaacaaaaa tttatattgc
    48781 gatagatgac ggaggacgta tcttctttca gattgcgaaa gtaaactatt gaaagcagct
    48841 actcgtttct cccctcgttt ttgtaattgc gtcacttgat cttccattag agaaagcagc
    48901 ggtgacacga tgatgaccag accttccatt aaataccccg tcaattgata gcaaaggctt
    48961 tttcccgttc ctgtaggcaa aatagcgaga gtatcttctc catcaagcaa cgcctgaata
    49021 atttcttctt ggccgggacg aaatgaatca aatccaaaat atttttttaa ttcatttatt
    49081 aacattctgt atcgcttcct ttctcaatag atagatttga tacaaacgaa attctccata
    49141 atctaattgt ccatctacat tccattcgct aaagcgccac tctcgcacgg aatccttatt
    49201 tggttccaaa cgttcgattg tttctgcaga gagaatccac tcgtatggaa aatcatcaaa
    49261 aaacaaggcc cactcaataa gatgatctgt tactgttcct ctttttaaat gacggattgc
    49321 catgatctgg tcaattgtct gaccattcat aaacatctgc ttggtgatca tcatgctttg
    49381 attgaaattt tgctgtaata atgaatcgat caaagcgtgc caaagcgaat ctggccgttt
    49441 ttcgatttgt gctaacaaaa ggtggataca tctgctttgt tgcagataaa ccgctgactc
    49501 atcattgttt tttgccagct gataaggaag cagccctgtt cgctcatttc cagataattg
    49561 gttagctaaa aaatctgctg cttcttctgg tatctccgaa aacagctgca ccaaactctc
    49621 atacgcagta tcaatcaaaa tttcacgtgg caattggctt tcactaagcc atttttttag
    49681 ttggaaagta taaaaaggac ttgtctctgc tggaagatag tcttgaatac ccgttgccag
    49741 attagaaatt acttgaacag caaatttaat gagccgccaa ctagtttccc ctgttctgcc
    49801 atagcggtca aaacgcaaac cagtatgttc tatttgatgt tcggacaaca tatcagcccc
    49861 tgcaggcgtt agctttgctt cattttcatt tatttcgatc caaccttgct gcgcaatttt
    49921 ttgcaaaatt tgataaaact tatcctgctt caattctgga aaactgttat ggataaacaa
    49981 aagttcgtgg gtaaatccat aaataagtac tgaagtcgtt cgttttccca caaggagttg
    50041 gtaaagagaa ctaatccgca gcttgtgtcc tgatgaaaat agcgataaaa taaaaacaat
    50101 catattattc tccttaagtt aggatggtta gaaatgtcac tactatgtaa acttgtacct
    50161 gaacgctgta ttgcatgcgg tctttgtcaa atcgaagcgc cagatatttt cgattatgat
    50221 gaagaaggaa tcgttttatt tgcccaagaa ccagaggctc atcaacagtt cgtgatgccg
    50281 gaagaagaaa catatgtaaa aaaagcgtat caaaaatgtc cagttcgagc gattttatta
    50341 gaaaataaaa tataaatata aacgaaactt taaaaacgta cactaagttc tctaaacttg
    50401 aaaataagcc gggatctgtt tctcttgttc cgtgaaatcc cgacttattt tgaaagattc
    50461 atttgctatc tatagttaat tgactagttt tttcacggac cctataggac ccatttatat
    50521 tatttcaact ttttcagcca gttttccaat gtattgacga tcacagtttg ctggtctgcg
    50581 ttagaaattt ctgcctctcc atctcctttt tgctcaccat agctgccaaa acctccatga
    50641 tttccgccat tgattgatac atactctgtt ctttccggta aatactgctt agcttcttga
    50701 taagcttcct gattcaatac tttatctttt gtcgccgtga tcgataaaac aggaacagct
    50761 gattctgtca atgttccttt tttgtcggga taacttgcca tgaagaatac cccttttaaa
    50821 tgtttgtctg aatgattctt attgacataa cgacttgcca ttacgccacc taatgaatgg
    50881 ccaccgatca cataatcgga atttggaaaa tcttctttga ccttatccgc tttgtttccg
    50941 cccatcactg ctagatcaag tggcattttg acaatagcga ctgaaaagcc gttttttgct
    51001 aaatcttgcg cccaaatact ataactttct ggctctacga gagcaccagg ataaaaaata
    51061 agtaatggag actttccatg attctttttc tctccttcaa aaaataagta atcttctttg
    51121 ttttttcctt cttttaccaa cacttgcgct tctgacgatg gttcgtacgt attttgctgt
    51181 atatagtaat aaccggctcc accgagtaat cctaaaatga tgactaagcc aattagtatt
    51241 cgtctaaata ttttcacttt ctcccacctt ttctatctga gtgacccctt tttcttaatc
    51301 aagtatgatt ataccttaaa aaaataagag agaaatgcaa actttccagc atcaaataaa
    51361 aaaagacgga atatccattt gaatatccgc cctttttgta tgtttatttt tatttagcac
    51421 ataaacgata cacagcttct gcataaattt ccattgcttt atacatacta cttaattccc
    51481 atcgttcatt cgcctggtgc ataaagtcag gagtatcagg gaacatcgca ccgaatgcca
    51541 cacactggtt catcgtacga gcaaaagtag cgccgcctga tacaaacggt tcagtcatgt
    51601 ctccagtaat atcgcgatat gtccccaata gatttttcac taactcactg tctagtggca
    51661 cgtataatga atcaaggaag tcaaattctt cataagtcaa cccatatttt tctgctgttt
    51721 tagttaattt agctactaaa tcatctttct tgaatgtaac agggattctc atatctacac
    51781 cgatttttgt ttgttctggt gtgatttcca aacttgcaaa gttcatcgtt agctcgccgg
    51841 attgttcatc ttctgttttg cccacgacag cttcaccagt tgcattttct tgaactaatt
    51901 ttcctaagaa attgattggg ccaaaatcaa atacttctga aagagcaatt gccagacgag
    51961 aaatagcatt cactccttgt gctgcatctt tcgcgtggat acttttacca agaacgacga
    52021 tgccttcacc ttgttcttcg aaatcgaatt cgtgtttttt caatgcttct tttacttcag
    52081 aaagcttttc tccagagtaa ggagctgcat caggaacaac attcaacgct cctccagctt
    52141 ttacagaaaa ctcgttcgtt cctggtcctg tcaagtaagc ttgcaataag cctttttctg
    52201 cataaattaa aggaaattca gcatctggcg caaatccttg cgtaattcct tcttcttttt
    52261 cgttatattt ttctaaacaa cgccataaat tttcttcatc tgtaccaaag ataaaacgaa
    52321 tacgtgtctt gaattccacg ccagcatcaa tcaaagcttt gacggcatac attgccgcaa
    52381 tagaaggacc tttgtcatct tgggatccgc gaccaactaa ccagccgtct tttatagttg
    52441 gatcgaatgg ttttgtttcc caattatttt cgtcccctgc tggaacaacg tccatatgac
    52501 acaagatacc gaataattca tctccagagc caatctctga ataaccgtaa tagccttctg
    52561 gatctttaaa tgttctaaat cctagctttt cactgatttc caatacttta tccaaagctt
    52621 caatcacttt tttaccgaag ggatgtcctt gtcctgaatc tgcttcatct agtacggaag
    52681 gaattcggat caattcgctc aatgattcta atgcttgttc atgatgttgt tctgtcacaa
    52741 attttttcat gatattatcc tcctaatatg ttaaaaaggg tcatgtcaaa ttcctccgac
    52801 acaacctctt cttctcaaat aatgacgttt taagcctgct ccatcagcaa gcagaatgtt
    52861 cttaacaaat gatgaaagaa aaaacactca ctgaatggag ctattgactt atccacgtgc
    52921 ttatcgatag ccgtttatcg gtttcttcat tcttatgata gaccttgtac ctgtatttgt
    52981 ctaggtttag aacattgctg ctacacctag gatcacacag atggctacaa ataatatcgc
    53041 aattaattta ccggtaaatt tccaccacac tgaaacatcg aaacgagcaa ttgctacagc
    53101 acccattacg atagcagatg ttggtgtaat taagtttact aaaccagatg cagattgata
    53161 agcagtgatt actaaatcct taccgacgcc ggcaaattca cccataggtc ccatgattcc
    53221 cattgttgca gcagctaagc cggaagttga aggaatcaag aatgacatag ggatatagaa
    53281 aataaatgta ataatgatga atacacttga agataatgaa cttaatcctt gttcgcccca
    53341 atgtaagatg gtgtctgtga ttaatccatt gttcataacc acttggatac cacgagcaac
    53401 agctactacg attgctacac cgatcaaatc agaagcacca gctaaaaatt cagagacaaa
    53461 ttcagactct ttcatgccat aagtgattgc tactacgatg gacattacta agaataacat
    53521 agtaatttct gggaagtacc agtcacctaa tggaagcatg cttttaccta gaaccgtacc
    53581 tagtaccgga aggtttgtta accagtttgt caatgattca aaaatcgtga aattcgcatt
    53641 caaggttgtc caaggaatca aactgatgat catgatacca aaagtaataa agaacaatac
    53701 attaacatgt ttttgttttt tcgtgattac ttcttgacga ccgattgctt caatatcgaa
    53761 atgcttcata tcttctttac gttggttgta tactaatgat tttgttggat ctttttcgat
    53821 ctttgatgca taacgataca catatacaat agaaatagct aacaaaataa cgaacatcaa
    53881 gaatcgaaga ccaatacctt cacccatcga aatcccaaca gcttgtgaag ctacgcctgt
    53941 tgcaaaagga tttaccgttg aagctagaca gcctacttga gaccctacta atacgattgc
    54001 tacagctact actgtatcaa agcctacagc catcatgact gggatcagta aagcatagaa
    54061 tggaatcgtt tcttctgcca ttccgtaagt agagccgcct aatgcaaata acagcatcaa
    54121 tactggaata agcatttttt cttttccttt gaatttcttc acaactgaag caatcccggc
    54181 atctagtgcc cctgttttgt tgataatacc taaaaatcca ccaacaacca aaatgaagaa
    54241 agagattggg attgcagcag gtgtttcttt agttagctcc cccgctggta cacctagcat
    54301 cccattgatt ggcgccatga aaatatccca cagcccttga ggattttgtt gtactcgttc
    54361 atatgatcca gcgacgatgt tgcctgcatc gtctacacta tagtgcccag ctggtataaa
    54421 ccaagtaagt acagcgatta ctgcaataat aataaataag accgtatacg ctgaaggcat
    54481 ttgaaatttt ttcttttttg tttgttccat cttcttcttc ctttcttttc cgttagagac
    54541 aacaattttg gctctaatgt tattttatca aaaagagtat cagaacaaaa ataataacac
    54601 gcattttgta aaacttttgt tcaaaaagtt cgtttttccc tattccctcc tcttttttca
    54661 ggtttttaaa gctttcttct ttttcattgt agcgtttcct tttttttata aacaaacatt
    54721 ttcgtctttt aaaaggagaa gaatttcaca tttgcaaaga attgattgat ttttatgcat
    54781 aaatcaaaaa aatactcaaa acaaacaaat aaaacgttta cattattgca tgtcttgtat
    54841 ttttttccaa aaaaaaagac caagaaattt cttgatcttt ttgatattta tttgactgct
    54901 gtatgacgtt gttcgaactg atgttgtttt cgacttaacc atggaagaag ttgcgcatgc
    54961 agcactaaaa agacagcact cacgatgaat cctttgatca agttgaaagg aacgatccca
    55021 atcagtacat agtttgctaa agacatgccc agcatctgtg ttgcgttcag ccctaaaaac
    55081 attaagtaaa gaggcgtaat aacgaaataa tttgcgatac tcataaaaat agttagagcg
    55141 atggttcctg taatgacacc taaaaatttg tttcttcctt tatgctgttt ctttacacta
    55201 aagaaataat aaatcgggaa tgtaaagatc gttgttgata agaaactagc agtgtctcct
    55261 accaaattat ccggagaaaa acctgtagtc agcagatgta atactgaacg caagaacgct
    55321 gttgcaactc cggccgctgg accgaataag aacatgctga tcaataccga gatatcacta
    55381 aaatcgatct tcaaaaaact aaaagccggc atgatcggaa aagcaaaata ctgtaaaact
    55441 aatgcgatcg cagcaaacat cgctactgca accattttct gtacacgggt gttcttcatt
    55501 tgaaatcctc ctgttaggac tcctcttcag cgaacttttt gacgtttctc tccaaatgaa
    55561 aaaactctat tttccaggga aaatagagtt agagttattt gatcaagcgt cgtcaaataa
    55621 gctctatctt ctttatccag actatactgt cggtatcgga atttcaccga ttcagctatt
    55681 tttttgcaaa ataggtcgtg gactataacc accggtcggg aatttcaccc tgcccctgaa
    55741 gacgaacctt atataatttt tgtttacata ttcattatac atatctattc aaaaaacgca
    55801 acatctaaca cttataaaaa gtaagtgttc attttgagtg gttcagcaaa ctgctgactt
    55861 cttttttgtt cagttcacgg taatccccag gacgtaggcc ttgtaaagtc agatctccgt
    55921 atttttcacg ttttagtttt tgcacaggaa gaccaactgc ttgaagcata tttttcactt
    55981 gatgattacg tccttcgtgg atcgtcaatt ccacgatact gtggtttgtc ttaggatcca
    56041 ctgataaaat ttggaattta gctggagatg tttttcgtcc ttcgatcttt attccttttg
    56101 tcaaaggtgc aagcatcgat tttgtagcga tcccttttac ctttgcaacg taaactttat
    56161 ccacttcatg ttttggatgt gtcaggcgct gagcaaaatc tccatcattt gtcagtagca
    56221 agatccccga agtatcgtaa tccagtcgac ctacaggata gatacgttct tttaccatcg
    56281 gcaaaagatc cgtaacaact tttcttcctt tgtcatctgc aacagcagaa ataactcctt
    56341 taggtttgta caataaatag taaccgtatt cttcttgata aattgggact tcatccactt
    56401 ccacagtgtc atgtttccca acttttatcc ctaattccct tacaacttgt ccgtttactt
    56461 ttacacgtcc tgcaaggatg aattcttctg cttttcgacg agaagtgatg ccagcgtggg
    56521 caattacttt ctgcaatctc tccattacaa ttattctcct ttattcattc catcaaagaa
    56581 tagatctggt gacacgtctt cttcaagtga aatttccatt tcttgtgtat caggcagttc
    56641 ttctaatgat ttcaaaccaa aataatctaa gaagtagcca gttgttccat ataatatcgg
    56701 ccgacctggt ccttctactc tacctttttc ttcaatcagt ttatgggcag ctagacgctg
    56761 taccgctcct gaaacctgaa ctcctcgaat ctgctcgatt tcagctcttg tgattggctg
    56821 tttataagcg atgattgcta aggtttcgac ggcggcttga gataatgtgt ttgatgtccc
    56881 ttgcgcatat cgcttaagta agggagccag ctctttttta gtcgttaata cataatggct
    56941 ttccgtttca aaaatatgca aagctgagtg cgagttttga gtgtaagatt cttttaattc
    57001 tttcactaaa cttgtgactt ctgcttcgct gagatttaac agtccgctta attcttcatt
    57061 actgatccct tcctcgccaa caataaaaag aagggcttca agttcactta atttcgtcat
    57121 gttcggtatc tcctactgct ggataaagca taattgattc atagttttct ttttgttgcg
    57181 caatgatctg cccttttttc atcaattcta gcaaagccat aaaagtagtg acaacttcac
    57241 ttttagaagg tgactcaaaa aaagaatcga aagtacagcc atgcgcattt ctttttatac
    57301 gctcttcgat aaataccatt cgttcttcga tcgttgtgtc atcgggcgtg atcgtttttt
    57361 ccactttttg agaaaatttc ttacgttcta agattttctg catggcacgg atcaaatcga
    57421 tttttttgac ttgtcctcgc ttcaacggct tttcagcatc cgctaaccca tcgatgttca
    57481 taggttcttt tgtgtaataa agacttcgtt cctctgcttt ttctgtcagt ctttcagctg
    57541 catatttata tttacggtat tctaaaagct gagtgaccaa ttcttctcga ggatcttctt
    57601 cccataattc ttcatcgtct gtgacgaatt cttggacagg aagcaatgtc ttgcttttga
    57661 ttgccatcaa tgtagcagcc atcactaagt attctcctgc aacagctagc tcgagtgttt
    57721 tcattgtatg gatataattc atatattgct ccgtgatcgt agcgatcggt atatcatata
    57781 tatcgatttc catttgctga atcagatgaa gcagcagatc gagaggaccc tcgaatacat
    57841 ctaatttaat attgatctcc gtcaactcat tcactctttc ttctttttct ttgctagcca
    57901 ttttccaatg taaggctctg tgatttcagt tgcacggatg gagacatccg ggtacttttc
    57961 ctgtaattca tctaacattt gaaagcccat attctcaccg cgataagacg gattcaaaga
    58021 aatgtcatgg atgatcagct gatcgttcgt tgcccagtaa ataccaataa tcccttgaat
    58081 gttggttgaa ccttctggcg taaagaaaaa aagttcataa tcgtcttcag attcgtacgt
    58141 atcaatctcc ttcaacaaag aagttttacc tgtcagtttc ttatggaaac tcaatagtcc
    58201 catagcaatt ttacggtttt ctttccggta atgtgttaac atcttctcac cttcctatga
    58261 ctaagccctt ggaaaatgct gcttataaac atccgtcatt cttttcttcg tgatatgtgt
    58321 ataaatctga gtcgtagaga tatccgcgtg acctaacaat tcctgtactg tccgcaaatc
    58381 agctccattt tctagcagat gtgtggcaaa gctatgccgc agcgtatgtg gtgttacttc
    58441 tttgtaaatc ccagcctctc gtactaactg cttcagattt ttccagattc cttgccgaga
    58501 aagacctttc ccatgattat tgacaaatac atgcatttca gatgcgtcct ttacaagtaa
    58561 tggacgtgct tcgtctaagt agcgttccag ccattggatc gcataatcac ctaaaggaat
    58621 gatccgctct ttatctcctt ttccaactgt ctgtaccagt ccaagagaaa gatgaagatc
    58681 tcccaatttc aatccaatca attcactgac gcgcatccca gtagcataca tgacttccaa
    58741 gattgcacga tctcttatcc ctaacgtttt tgtcgtatca ggtgcttcaa tcagcttttc
    58801 tacttctgtc agagtcaatg tacttggtaa tttctgtgct ttcttcggcg tatcgatatg
    58861 ttgcatcgga tcatgatcag tgatacgctc ttgccgtaaa aactgatgga atcttctgag
    58921 acttgagatc atccgagtaa cagttgctgt cgcttttttt tcttcatgca acttctccag
    58981 aaaacggatg actaaaaagc gatcgacatc ctgccatgaa gcaattttct gctcctgtag
    59041 aaaatgcaag tactgttcca aatcacgttc atagctttgt cgtgtattgg cggacaatcc
    59101 ccgttcaata ttcaagtagt ggagataatc gttgatttgc tctttcataa taatccccct
    59161 catgcctctc tatcatatca gataccgctt cattctgata gtctttttcc ataatctgag
    59221 caaagaaaaa gccgtctaga cactcagacg gactggtttc ttttttcttt ttcatagcat
    59281 tcctgacaga ttccatggaa cgttagccga tgatctttta ctaaaaaatg ataacgttct
    59341 tctactactt gctccacttc aagtaaaaga tcctcttcta cttcctcgat cgtgccgcag
    59401 ttcaagcata gtaaatgatg atggaaatgt ttggcgcctt cttttctcaa atcataacgg
    59461 gctaatccat cattaaaact tactttatcg acaattttca attctgtcaa gatttcaagg
    59521 gttcgataaa cagtagcaag tccaatttcc ggactctttt ttttcactaa aaagaagatt
    59581 tcttctgcag ataaatgctc tttctcattt tccaaaagca caagcagagt agcttctctt
    59641 tgcggtgtca gcttaaaccc cgcttcatgt agctgctgtt ttgttttttt catcgccgaa
    59701 atagcattca tgaccattta ctcaacccca acaatttata ataattacaa ataaaaaaag
    59761 atataacact atgttaatta aaattattat aagctagtta agaaaaacat gcaagggatt
    59821 ctcaattaga ttttttgact agttttgtca caccgttttc ttgtgtaatc aatccttgtt
    59881 tcaaaagatt acctaatgcg cgcttaaatg ctcctttgct gataccaaaa gctttcatga
    59941 tttcttctgg atccgattta tctgtaaagt tgatttgctg atctgcagaa cgttctaaaa
    60001 tggttaaaat catttgtgca tcatctgata tagcttcata tgctcttggc ttcaatgaaa
    60061 gattcagtac accgtctggt cggacaccaa tcactcgacc ttttatctgc tcgcctagac
    60121 gaggttcatt atatcgttca gaaggatgga taaacccaag atagaaatct tccgttaaaa
    60181 cgtacgtacc tgttaatttc aagcggtatg ctgttcctat cacatctttg tttttcattt
    60241 cttctgttcc atatttgctt aaagatttga atattttttc atctgctaaa ctgccccaaa
    60301 tacggtcttt tgcatcaact ctaagggaaa tcatcaagcg atcccctttt ttaggccata
    60361 gctctgtcat tgtcggcaat tcatcaagag atacgacaaa gtctttatct ggaagaccga
    60421 tatcgacaaa cgcacctaaa tcttttcgtg tagcagtaac gattccaaaa gcgtaatgac
    60481 cgatacggct tttaggaatt tgtgtagtga tccgattttc atgtttttga ttttggtaac
    60541 cgaatccttc aactgcttca ccgattttgt gttcaccttc tgatttgctt aagccaaagg
    60601 tttgtccgtt tttttggaca aagtagtttt tttcattttc atcaataata atacctgtaa
    60661 atatagttgc taataaatcg ttcatttttt tctccctcta tagtgaaagg ttttcttaat
    60721 acatttcttt gctatcataa cataatgtta tctacttgaa tagctgagcc ttggtttgga
    60781 aaggtgaaaa aaggagtatg acaacagttg tgtcatactc ccctttttcg aataaacggt
    60841 gttaaacggc aggctcacag cctctttcat ttgcctaaaa ttaattgctc ccatttcctc
    60901 cgaaccatcc agttttacag ttgatggtta cgatgtaaaa tggcaaagca ctttctccaa
    60961 tgcctttcat caatgcagat tttgttgaag tggcatatag attataatag ccttccccat
    61021 tttcgattct ggctttttct aatctgtaac ctccttcaac gatatatcca cgttggatac
    61081 atgtagcaat cactttgctt tggacacctg gtgcccacgt ccatgctgga ttagatgcat
    61141 cggcaatatc acttgctctg gcagatttga ttggctgatt cgctgagttt gaagaggaag
    61201 gggtcgagga tgccgcttct tgctgtgctg cttgtgcgac cgcagcttgt tgagcggctt
    61261 gagctgcagc tgctgcttga gcggcttgtt ttgcttgttc ttcagctgct tttttcgctt
    61321 cttctgcttt tttcttttcc tcttcttctt ttttctcttt atcagtgagc atcttatcta
    61381 cttgtttcaa ttgttcattc aactgagcag atagatctgt atttttcact ttttctactg
    61441 cttcttttgc cgtcttgtat tggtcgcgtg ttgctgaatc cactactttt ccgtctttaa
    61501 agactatagc tgttttttct ttagctgttt gcaattcagc atattgattt ttagcttctg
    61561 tttgtgcttg ttgaatcaat tttccaaaat ctgtgttatc cgtttcgatt gcttggatct
    61621 ctttatctgc tttcaacttg atgtcatctg ctagatgatc atcgacgatg gcaggtgcta
    61681 tgaaccactc attcactttt tggatatctc ggatcttaga ttggatatct tggtaagttg
    61741 tttctaactc tgaatatcct ttttcattct taagactgga taaatcattt tttagttttg
    61801 ataaatcttg atgaatcata tctgcacgaa taaattgatg gtgatcatct gtataaaagg
    61861 cagcaagttt ctcatgaagt tcttccattt gttgctgtct ttttgcttct gctgctactt
    61921 cggcttctac tttttgatga tggtcataat aagcccagct tcctccaccg agtagaagta
    61981 ctgctgctaa aacgagataa ggtacttttt ttgatttttt cttttgcgct ttatctggat
    62041 ctgttttttc taagtttgat tcttgatgtg ttttttcagt atctggcacg attgttattg
    62101 atttcgcttc tgcttcaaac agcttaggct ggttctcttc tttttctatt tttgcttcaa
    62161 gatcagtacc ctctttgctt acagattgat tttcagcatc ggattcacta tttatttcat
    62221 cgggctctgt tgactttttg tctgtgtttt cgagtatttc ttttttctct gcttccggca
    62281 gtttattcgt tttttccttt tctaattgtt tttcagtaaa tacttcagtt ttttctgtga
    62341 tatccgtttt tacgtctttt atttttactg tttctttacc tgtggcgttt tctctatcag
    62401 agtctttttc agtttcttgt tccttttctg gctctacttt gttttcctgt gcaatttttt
    62461 cactgatcat tttgagcagc tctttcgcgt gctcttcttt tgtatcttca cgatgtgctt
    62521 tgatataagc ggatagtatc gaattttctt cctcattatc gacgatttct ttcagttcag
    62581 ctactcttct tttttgtttt gattcaagcg tttcttcttg attctttcct gattcttccg
    62641 aagtctcatg aacagttgct ttcttctgtt catttttctc tatattagtt acttgatcgt
    62701 gagcaggtgg ttcttcgtct tctggttctg ctttttcgaa taattccatt acagattcta
    62761 gaggtaaatc tttcaattcc gaccattcga ttggatcgtt taattctgta tcaggaaaga
    62821 ctgcatcttc agttgattta tcctgcatag ctgcatctgt atttgtttct tcaattgatt
    62881 cttttatttg ctgatcttct gcagttattt gatgaccgca gtctggacag atagtattct
    62941 gctccatttt tttcgatccg caatttggac attttttcat cacttagcga tcggctcctt
    63001 tactaggttt acgctaccat ttaatctatc atattctata cgtcacgcca atagcttatt
    63061 tttaggattt acttaaaaat aatgaatgaa tttccttcct atagaaaaaa aagcttgaga
    63121 ctgcgtagtc tcaagctttt tttcttttaa aataaaccaa acagttttac tgatttctct
    63181 tctggtcaat agtccacgct tattctaaga aatcttttaa ttgttttgaa cgagaaggat
    63241 ggcgcaattt tcttaatgct ttagcctcga tctgacgaat acgttcccga gtaacaccaa
    63301 atactttccc tacttcttcc aatgttctcg tccggccatc atctaaacca aaacgtaagc
    63361 gaaggacatt ctcttcccga tcagtcaatg tatctagtac atcttccaat tgttctttta
    63421 gtaactcata agctgcgtgt tctgctggac ttgttgcttc ctggtcttct atgaaatcac
    63481 ctaagtgaga gtcatcttct tcaccgattg gcgtttcaag ggaaacaggt tcttgcgcga
    63541 ttttcaagat ttctcggact ttttctgttg gaagatccat ttccgcaccg atttcttctg
    63601 gcgtaggttc tcttccaagg tcttgcagca attgacgttg gatacgtatc aatttattaa
    63661 ttgtttcaac catatgaact ggaatacgga tcgtacgtgc ttggtcagcg attgctcgag
    63721 tgatggcttg acgaatccac catgtagcat aggttgaaaa cttaaatcct ttgcggtagt
    63781 caaatttttc aactgccttc atcagtccca tgtttccttc ttggatcaag tcaaggaact
    63841 gcatgcctcg tcctacataa cgtttagcga tacttactac taaacgaagg tttgcttctg
    63901 ccagacgctg tttcgcttct tggtcgcctt cttcgatttt caatgctaac gctacttctt
    63961 cttccgcagt taaaagggag acacggccaa tttctttcag atacatacga accgggtcat
    64021 tgatttttac accggttggc gcagaaagat cttctgtttt tgcttgttca gcttttttct
    64081 cagcactctt caagctgtga attgatggat caccattttc atccaccaca ctgatacctg
    64141 cgtcttcaat cttttggatc aatttgtcca tttcatctgc attcaaagtg aatggtgtag
    64201 caagctgatt ggataaatca tcatatacaa cttgtcgttt gggtttgttt tctttgatga
    64261 aagctgctac tgctgcttca tacgtttttt tatctgttcc atttgccata aaagaaggcc
    64321 ccccttgagt agctatttgg cttgttttaa ctgtttcgtt aaattgatga tttcaatcgt
    64381 caattcaagc tcaagctgtt tgtttccagt tcgtctggct tcttgctgca tttgtttttt
    64441 taatgttatt tcatcagcta agccagattt gctgatcaca tctagcaagt cttggatttc
    64501 tcgtggcgaa ctttcttctg ccaccggtaa atattcgatt tccaccacca accgccttaa
    64561 attttcatct tgcaaaaagt ctaaaaactc tgcaaggttg aattcataat gttgtgagac
    64621 atatgcatcg aataaaaaat aaacttctgc atatgcatca tgaataaact gaaattctcg
    64681 ttcttttaaa atattacgga cagaaacttc tttaaatgca cgatataata gaatctgctc
    64741 tgccttttct acttgcgtca aaggtctctt tttccgtgca gtttcttgtc tcgtaacctg
    64801 agtgtgttga ctttcctgag gttcttgacg attttgctga tttgtccttc gaagatcgcg
    64861 cagttgtttt tgtatcgtct ctctgccgat ctgaaactct gttgacagtt gagatagata
    64921 catatcttgt tcgattggtg aaacaactcg gctaagttca actagtaatt cttcgaggta
    64981 ttcgatcttg tctttttcat tatctaaatt tttcccttgt tttaaataac gtgatttaaa
    65041 agtaaagact gtttcgcgcc catgagccac taattcttgg aaagatgctt ctccatactt
    65101 acggagatat tcatcaggat caagcttttc tggaacgact acgacagaaa gtgctaatcg
    65161 actgttttgc cgaagcaatt caatccctcg attggttgct tcaaaacctg cgttgtctcc
    65221 gtcatagcaa aagaccaatt ctttcgtcag cttttccatt tgttggatct gttgagctgt
    65281 tagacttgtc cccatagaag ctaatccagt tttgatacca gcctgccatg cagcaatcac
    65341 atccataaac ccttcgaaca acaaaatttc tccgtttttc cggatatcgc ttcttgcttt
    65401 atcaagatta aataaaactt gtcttttatt gaacagttct gtttctggac tattcaagta
    65461 tttgggctga tctttttcct gccctttttc aggtgcaagc cagcgtcctg aaaaaccgat
    65521 cgtctggcct tgagaattac gaagtggaaa catcactctt tgataaaaac gatcagacat
    65581 acggccatca tcatgcgtga caaacaatcc agagcgttct aacaattcat ttgaaattga
    65641 ttcattcttg aatacttgct gaagaaaaga acgctcatta ggtgcaaaac ctatgttgaa
    65701 ttcttcaatt aattctaaag acaacccacg actttgcaaa tattctaaag ctggctgtcc
    65761 agcttttgta tgcaatagca tgtgatgata gatttccgct gctttttcat gagcagcaat
    65821 cagtttcccc gtagcagatt caggatttcg gacagctata gtacgctgac gccattgatc
    65881 ttctaacggt atctgttcga agtctgccac ttttaccact gcttctggaa aacttagacc
    65941 ttctagttct tgaagaaaag taaacacgtt tccgccttta ccacaaccaa agcaatgaaa
    66001 aatctgcttg tcttctgcaa cagaaaaaga cggtgtacgt tcttcatgaa aaggacacag
    66061 ccccaagtaa tttttaccag actttttcaa ctggacatat tgtccaatca cctcaacgat
    66121 attcgtttga ctgcgtactg tttcgataac ctcttgagga atccgttgag ccattttttc
    66181 accccctgct ctattgatat cttttgggtt taacagaaga tatttcagga gaataaccgg
    66241 taaaaaatcg cactaactga aatcagtgcg attttaaagg acacaattat acatgtttga
    66301 ttgtatcata tttcattgct ttttcaagta taacacattt ctatctcatt aagcatcaat
    66361 ttgctactct tttcagtaac tgattttgaa tatcagcctt ccagtacaat actcgaagaa
    66421 caatatttac gagcatgaat acaaagaagt atcctacgct ctctcccaac cctaagttta
    66481 cttccaaaat tccatggaac agcattgcag ctacatatat ccctacgatc gtcgttactg
    66541 ctaggaagat gcgcaatggg ttgaagggat aacaagcttt tacaactgcc atgcaactga
    66601 tcccaatcag caagtaatac atcaaagtag tagtttcagc atttccccat gcgaatgttt
    66661 ttccaagtag atacaccgca atgatattca ctactacaag caaggcattt ggtaaagcat
    66721 taataagtgc tgtaggcaaa taacggtaag tgatttttct tttatcttgt tcaaaagata
    66781 aaaagaagga aggataccct tcaatcgcta aatcaatcag cgtgatttgg atagggataa
    66841 acggaaatcc aattgctgta acggcacata taatcgataa aataaatgaa taaatcgttt
    66901 tgataaaaaa gatgcctgag actttcgtca cattgttgac cactcggcgt ccttcaaaca
    66961 ggacttctgg caatgtagta aaatcagagt ccaacaatac cagattcgca atctgacgtg
    67021 ttgctccatc accttctgcc atagcaatgc tgcaatctgc ttctcttaac gccaaaacat
    67081 cattcacacc gtcgcctgtc attgcaaccg ttctgccgct ttctttcaat tcatttacca
    67141 gcaatttctt ttgctgcgga ctcactcgtc caaaaaccgt atagcgatga gcagcttctc
    67201 gcacttccat ttcttcagta atttgagaaa gatcgacata cgcatcataa ttcggtaatc
    67261 ctgcacgtct agcgatattg gagacagtaa aaggattgtc tccagaaatc actttcagat
    67321 ccacaccttc ctcatgaaga taagctagtg tttcttgagc gttttttcgg attggatcat
    67381 cgatttcaaa cagagcaacc ggctcaacaa atgctggttt ttcttcgttc aattgctgat
    67441 ttggagcgat ccccaacata agtacgcgat agccattcgc ttgggctgtt tttagactgt
    67501 ccggaagccg atcttcttcc accaaacgtt caggcgctcc tacgtacacg actcccagtt
    67561 ctggaaattc catcgcgccc catttgcgat cagaagaaaa tgcgactttg tttgtcacat
    67621 cataccgatt agaaagctca aaataatctc tcatagcccg catagtgatg ttgttatcac
    67681 tgctttctgc cagatacgtc cccataattt ctgggatggc tttctcgtac agcggatgta
    67741 gtgcttctac ccgctggata ctcattttcc cttcagtaat ggtccctgtt ttatccaagc
    67801 ataatgtatc gacatgggcc agcgtttcaa ttgaatacat gtcttgaaca aggattcgtt
    67861 tttttgccag tttggttacc cctgtcgtta atgcaatact tattagcaat accaaaccct
    67921 ttggaagcat gcctaacaaa gctgctgcag atgcaataac agaaattttg atagaagcat
    67981 cgcggatgaa cagtgcttcc aaaaatagta tgatccccaa aggaataatc acgaaactag
    68041 tgaatttgga tactttgcga atcgaatcta caagttcaga acgaatagct ttgtgagtct
    68101 ttgcctcgct agtcaagcgg acagcataat tatctgcacc tactcggata acttcagcca
    68161 tgacagaacc gcttgtcaaa aagcttccag aaagcaattc agctccagtt tctttgacaa
    68221 tcaaatcaga ttcgcctgtc aaaagagctt catttgcttc tgcccgtcca gatataacct
    68281 gtaaatcggc tggtatctgt tctcctgctc cgatttttac caaatctcct aataccagct
    68341 ctttagtaga aatcgattgt tctttaccgt cccggatcac tagtacttct gattgggaaa
    68401 caatcgataa tttccgtaca agatttcttg catgtatttc ttgataaatt ccaataacga
    68461 tattgacgac aataattgct aaatacgcca tgttggaata cgcacctaca aataacagac
    68521 aaatcccaat accaaagttc agaaaattga ataacgtcaa tatattatcg ctgaatattt
    68581 gttttgtact ttttgatgca tcttcctggt agtcattttg ctggccagaa gccattttct
    68641 gaactacctc ttgctgtgta agtccattca ttttcttttt ctccattttc cgccaacttt
    68701 caaaaataat cacgtgttta agtctagcgt gttccaagat taaaaacaac ttgtttttat
    68761 aaaatttata gaaaaacaca cgaatgattt tacaaaaaag atgccaacct ttttgtttga
    68821 atcacccgtg tgctgttttt attatcaatc tatctttact tatattgttc ctccaaacgg
    68881 ctcatccacc aacctagtgc tgtaccggct ataaaaagaa caggacaagc tagtacccca
    68941 gcaactccca atcccgcgta atcggtagga atgatcataa tcgttgaagc aaaaactaca
    69001 cctaaaatga aatggaatag cttgctatat gcctttgaga aaatgtaatc catgattttt
    69061 gacaatccaa gaacacaaag aatcccacca attccgatcg gcacaatcac ttcaagtcgt
    69121 acttctttaa atccgtcagc catcgcttta tacaatccca tatacaccaa aaaattagaa
    69181 gggctcaatc ctgggacgat catacccaaa ccaatcaatc cgccggcaat cagccatgtt
    69241 ccaaaattct gatcgacttg cccgtcaaat aattgagcgc cgtataataa aaacaaataa
    69301 gccagaacaa aagtaacaac catgatgatc acatcagaag tatctcttcc ttttttccct
    69361 gcttctttcc ataacgcagg aacagttcca gcgatacatc cgacaaaaaa ccataatatc
    69421 gttgtttcat acgcacctaa taaaaaactg actaaaaaag aaagtagaaa gatgcctgtc
    69481 actccgccta gaccaacagg cagaaaaaac aaaacatttt ctttgaaatt ttttgtaagg
    69541 tgggctaaaa aagaaatcag ccgctcatac atgccaaaga cagctgctaa tgcaccaccg
    69601 ctgactcctg gcaagataaa tcctgaaccg ataaacattc ccttcacaaa ccgtaacatc
    69661 cagtctttgg ttgtgtaccc cacttcttga ttttccattt tttgcctttt ttccactatt
    69721 tgcgtactcc ttctttcatt taattttatt catgattgaa catttcctgc tattttactt
    69781 aaagactttt tcataggtct ctattatttt ttcattcatt ttgacaatat caggataaaa
    69841 catgttacct ccattatgat agagatagcc gccattgtaa agcaaggact ggatccgcca
    69901 atatcggtat tgtttccctt cctcatttcc taaaagtggg ctcagcacat ctttagaata
    69961 cgcttctgct aattctactg tgttttctcc gccatgattt tttacatagg caatataatc
    70021 tttcccaaaa ttatatgctt ggacagctgt ccataaatcg catccttgtt tctttgcttc
    70081 tactatcatt tccgccaaat atttgacacc ttgatcaata ctgtcagctg gattatcaat
    70141 gatctcagct tctccatgca gactttccga gctctgcatg agatctgtcc cttttccttt
    70201 tgattcggtc aatattatcg ccttgatcaa attctcgtag ttttccatat gataattttt
    70261 tgcagctgtt gttatttcta tttgatagct ttccactcgt tttaccgaac gataagttct
    70321 tcctcataaa aacaacaccg caccaataac cgtcagaatg atcatgtaaa aaagaatacg
    70381 gataatagat aatcgttttc gcttcatttc atcacctagt tattttgaca tcttttttta
    70441 tcttactatt tctttatctt cttggctatt tcttccttga tttctgtgta cgccttaaga
    70501 aaaaatgaat tttcctgtat tttttgacat cttacgaaaa tgatcgttgt ttttcagtaa
    70561 aaacaattgc ccaatgactg atataatcgt tatactttac acagtaggtg attatgtgaa
    70621 aaaagaaaag aaatatgcaa aaatgccaga tgggagcgaa atttactatg aaaagagcgg
    70681 tcaaggcttc cctctctttt tactgcatgg caatgacggc agcggccgct ttttttcaga
    70741 acaggtccct gtactcgaac gctactatac cgtgtatcta gtagatagcc gaggacatgg
    70801 tcgttcgaca aacgaagcat ctatgttgaa ttttcagtta atggccgagg atctgaatac
    70861 gatcatgttg ctggaaaaaa tcgatcaggc agattttctt ggatttagtg atggagcaaa
    70921 tctagcactg gtatttgcca gcagcttccc taaaaaagtt catcgattga ttttaaattc
    70981 tggaaacacg cttgttaaag gcgttcgttt ttctgccaga gtcatcagta atattcatta
    71041 tgcatgggta tggttactct ctttatttcg cccaagtctt cgcaaaaatc ttttagttat
    71101 caaacttttg ctgcatgaca tcgggcttac agaaaatgat ttaaaaaaaa tcaactcccc
    71161 cactttgatc atcgtgggaa aaaaagatgt aatcaagcta aaacattctt tgtatatagc
    71221 taaaactata ccaaaagctt cctttgtgct tgtgaaagaa caaggacatg aattagctag
    71281 aaaagatcct gaacgcttca atcgagaagt tcttcaattt ctttctgaaa cataactatt
    71341 aggaatagaa ggtgatttat ttgttaaaaa ataccataca atggctgaaa aaccacctag
    71401 gactgttcaa aacaatcttt ttgatttctg tcattgtcat tattgtcggt gaattgatgt
    71461 ccatcggtaa aactctatct atccagcaat taggagaaac gtttgccact atacctgtat
    71521 ggaaaagcgg actaatgcta ttgattggtc ttatctctgt gttacctatg gttggttatg
    71581 atattatttt gaatcggatg cttgaacaaa agcagaatcc tcgttacctg tttgagacaa
    71641 gctggctgat caatacgatc aacaatattg ccggtttcgg cgggtttgtt agtgtggggc
    71701 ttcgctctga actatatgga caaaagaaag agagcagaaa cgtgatccaa gcattatcga
    71761 aagttttctt gtttttgatg gctggacttt ctatttacag tctgctttct tttttacttg
    71821 tagtcttcac gccagttgct ccttttttga agcaatattg gatatggttg atcggcggcg
    71881 gtctatattt tcctgtcgtc tttttattta ctactttcaa aaagaaagga ttccttggtg
    71941 gtacctcact taaaactcaa ggacagctat tgctagtttc attgttggaa tggaccggtg
    72001 tcctgtttag tttcctttct gttggttatt taatggaaat ccggattgat ctgtggcaaa
    72061 caattccatt attcattgct gcttcagtga taggaatcgt gtctatgatt ccaggagaaa
    72121 ttgggagttt cgatgtaatg atgattatcg ggttgtccgc tattggtgtg ccaagagaaa
    72181 tagtcgttgt gtggatcttg ctctatcgcc tgttctacta catcattccg tttttgattg
    72241 gaatcgtctt tttcttcaaa aatatcggtt ccacttttga tcagcgttat tctggtatcc
    72301 ctaaacaatt agcaacagaa attgcgcata aaatcgtggt tgtcttatta tacttttctg
    72361 gaatcatgct agtactttct gccactatcc cgcaagcttt tacagagttt cgctggcttc
    72421 atagtttgaa cccattgaaa tttcatttta ttatccaatt tcctagtata ctattgggat
    72481 ttcttttaat cattatgggc agagggatcg cagcacgtgt gaaacgagca tatttgccta
    72541 caatcttctt gattgttcta gctttacttt atgttttatt aagtgatttt agtttcacac
    72601 cggtggtttt cttgtctatt ctgcttctga tcattcttgc ttctaaaaac gaattatttc
    72661 gggaacaatt gatctattca tgggagtggc gtacgatcga tggaatcatt attggtgcac
    72721 tgtccttgct ttacatcgtt attggggtat acaatctgcc ggatttccct catcggcgtc
    72781 accattttat ttcctttttc ttatttcctt ctgaaaagat ttggttttcg ggattgttgg
    72841 ccatcatcgc tgttagtttc atgatcgttc tatttgttca tttccttcaa ggagaaaaga
    72901 aacagattgg cgaagccttt aatgaagaaa aggccctgaa gatccttaca acttatggag
    72961 gaaactccga tagtcaattg atcttcttaa aagataagcg gatgtttgcc tatgaaaaag
    73021 acggtgaacc aactgttctt ctccaattcg cttgcttcaa taataaatgt attgtaatgg
    73081 gtgatccttc tggtaagaaa gaagattttc cagaagcaat cgaagctttt atcgaagaaa
    73141 cagatcgact atgctattta ccagttttct acgaaacaag cgaagagatt gttatgattt
    73201 tgcatgaatt cggctacgat ttcattaaga tgggggaaga agcttatgta gacctgaata
    73261 gttttacaac ttcagggaaa aaaatgaagg gcactcgtgc tgtcttgaat cggatcgaac
    73321 gagaaggatt tacatttgat gtcttacaac caccattttc tactgagcaa atgagtactt
    73381 tcaaaaatat ctctgataat tggttgggtt cacgaaagga aaaagggttt tctttaggct
    73441 ttttctctga agattatcta caaagagcgc ctattgcagt tgtcaaaaat acccaagaag
    73501 aaattgtcgc ttttgcaacg atcatgccta cttacacgaa caataaagta ggaacgattg
    73561 atctgatgcg atatgattct aaaaaagcac cttctggcag tatggatttc ttgttcttgc
    73621 atttgtttgc ctatatgaaa gaagaacata tccaatggtt caatttaggg atggcccctc
    73681 tttcaaatgt cggaacttcc agaaagagtt ttttacagga aaggattgca tacttggttt
    73741 acgagtttgg ttctcatttt tattctttcc atggattaag agaatataaa agcaagtacg
    73801 ctacaaactg ggtctcacgt tatactctat attctagaga tagttggatt gcatatgtta
    73861 tgattgcttt gctgattatt gataatgcgc cagttgagca gacacaaggt agaataagtg
    73921 gtttgaaaaa atggatacga agaaagtatt aaaaactgca taccgataga tgaacactct
    73981 catgatgaat aaaagagact gaagtaaaac gaaagttcat gcttctacaa tctcttctta
    74041 tacaattgaa gaacaaagag cggtccaaac agaaatcctg tttggaccgc tcttttattt
    74101 taaacatttt tcattaccaa aatattcgag tcgcgtttct tatgcttcga tctctgtaac
    74161 gatacctgaa cctactgtcc gtccgccttc acgaattgag aatgtcgttc cgttttcaac
    74221 agcgattgga tggatcaagt caacatcaat cgtcacgttg tcaccaggca tgaccatttc
    74281 tgtatcttct ggcaatgtga ttgttcctgt aacatcagtt gtgcggaagt agaactgtgg
    74341 acgatagttg ttgaagaatg gagtatgacg tccaccttct tcttttgtca acacgtacac
    74401 ttctgctttg aattttgtat gtggagtaat ggaacctggt ttagcaagta cttgtccacg
    74461 ttcgatatcg tcacgttgga tcccccgtaa caacacgcct acgttatccc cagcttcccc
    74521 gtaatctaac gttttacgga acatttctac acctgttaca actgcttttt gtgtttcagg
    74581 tttgatccct acgatctcta cctcatcacc gacacgaaca gcaccacgat caatacgacc
    74641 tgaggcaaca gttcctcgac ctgtaattga gaagacgtct tccactggca atagtaatgg
    74701 tttatctgta tcacgttctg gtgttgggat atattcatct actgtatcca tcaattccat
    74761 aatagcagct tcagcatctg gatcgccttg caatgctttt aatgcggaac ctttgatcac
    74821 aggagtgtca tcgcctggga aaccatattc gctcaataac tcgcgaactt ccatttctac
    74881 caaatcgatc aattcttcat catcgactaa atcaactttg ttcaagaaaa caatcaggta
    74941 ttttacacca acttggcgtg acaataaaat gtgttcacgt gtttgaggca tcggaccgtc
    75001 agttgcagaa acaactaaga ttgcaccatc catttgtgct gcccctgtaa tcatgttttt
    75061 cacatagtcc gcgtgtcctg gtgcatcaat atgcgcgtag tggcgttttt ctgtttcata
    75121 ttctacgtgt gcggtattga tcgtaatccc acgttcacgt tcttctggag ctgcatcaat
    75181 actagcatag tcttgaggat ttgctaggcc ttttttgcct aatactgtag tgatcgcagc
    75241 agttaaagta gttttcccat ggtcaacgtg tccaatcgta ccaatgttaa cgtgtggttt
    75301 acttctgtca taatgttctt ttgccataat ttacaaacct cctaaataat atagatcact
    75361 gaaaaatttc agctctcacc tttttattat agtctttttt tgactaaatc aaaaagaata
    75421 cgtccgactt actttttgta agattcgaac taagcttatt atacgtcaat attggtcaaa
    75481 gtcaactaag agcactcgtc tgtcttatac gcgaaaaaag taccttttat aaataaaaaa
    75541 atgtgttttt tgagaaaaac ttcaagatag tccttaactt tcttttgaag caataagaac
    75601 aaaatgagga aaagtttaaa attcagctgt tgccaacaac tttcaataga cgaaatcttc
    75661 tatttcgtta ataataagga aagaggtgaa gtacatgaaa aaattaattg gcgtacttgt
    75721 cgttttaggc atatttctcg gcggaagttt tgcagcttat cattattttt acggcggcga
    75781 agcttattat acaaaaatca cgacgaatgg cgaaatgtca gctacccaag cagataacgg
    75841 agagaaattc acgacttata cctacaagca agcggcttat aataaagatg gtgaaaaaaa
    75901 acaggtgacg cttcgcgaag aaagagaaca tccattaaag atgaatgcgt atcttaaact
    75961 caaagtcaat cctcgtaaag gtgtattaag ctgggaagaa ataaaggctt ctgaagtgcc
    76021 taaaaatgcg gcagaaaaaa tcgataatta gtaaatggtc ttaaaatttt attagataaa
    76081 a
//
LOCUS       DAZKOU010000002        75350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000002 DAZKOU010000000
VERSION     DAZKOU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 75350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.002"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..933)
                     /locus_tag="ACG827_000071"
     CDS             complement(<1..933)
                     /locus_tag="ACG827_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0482247.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSSYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEE"
     gene            complement(1188..1697)
                     /gene="mreD"
                     /locus_tag="ACG827_000072"
     CDS             complement(1188..1697)
                     /gene="mreD"
                     /locus_tag="ACG827_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0482248.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(1701..2555)
                     /gene="mreC"
                     /locus_tag="ACG827_000073"
     CDS             complement(1701..2555)
                     /gene="mreC"
                     /locus_tag="ACG827_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0482249.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(2710..3348)
                     /locus_tag="ACG827_000074"
     CDS             complement(2710..3348)
                     /locus_tag="ACG827_000074"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0482250.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3349..3999)
                     /gene="rpe"
                     /locus_tag="ACG827_000075"
     CDS             complement(3349..3999)
                     /gene="rpe"
                     /locus_tag="ACG827_000075"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0482251.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4012..4911)
                     /gene="rsgA"
                     /locus_tag="ACG827_000076"
     CDS             complement(4012..4911)
                     /gene="rsgA"
                     /locus_tag="ACG827_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0482252.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5076..7145)
                     /gene="pknB"
                     /locus_tag="ACG827_000077"
     CDS             complement(5076..7145)
                     /gene="pknB"
                     /locus_tag="ACG827_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0482253.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7142..7882)
                     /locus_tag="ACG827_000078"
     CDS             complement(7142..7882)
                     /locus_tag="ACG827_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0482254.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(7895..9253)
                     /gene="rsmB"
                     /locus_tag="ACG827_000079"
     CDS             complement(7895..9253)
                     /gene="rsmB"
                     /locus_tag="ACG827_000079"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0482255.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9253..10191)
                     /gene="fmt"
                     /locus_tag="ACG827_000080"
     CDS             complement(9253..10191)
                     /gene="fmt"
                     /locus_tag="ACG827_000080"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0482256.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10184..10675)
                     /gene="def"
                     /locus_tag="ACG827_000081"
     CDS             complement(10184..10675)
                     /gene="def"
                     /locus_tag="ACG827_000081"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0482257.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(10690..13098)
                     /gene="priA"
                     /locus_tag="ACG827_000082"
     CDS             complement(10690..13098)
                     /gene="priA"
                     /locus_tag="ACG827_000082"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0482258.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13351..13656)
                     /gene="rpoZ"
                     /locus_tag="ACG827_000083"
     CDS             complement(13351..13656)
                     /gene="rpoZ"
                     /locus_tag="ACG827_000083"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0482259.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(13661..14275)
                     /gene="gmk"
                     /locus_tag="ACG827_000084"
     CDS             complement(13661..14275)
                     /gene="gmk"
                     /locus_tag="ACG827_000084"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0482260.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(14762..15046)
                     /locus_tag="ACG827_000085"
     CDS             complement(14762..15046)
                     /locus_tag="ACG827_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482261.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15064..15954)
                     /locus_tag="ACG827_000086"
     CDS             complement(15064..15954)
                     /locus_tag="ACG827_000086"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0482262.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(15974..16192)
                     /locus_tag="ACG827_000087"
     CDS             complement(15974..16192)
                     /locus_tag="ACG827_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0482263.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16162..17862)
                     /locus_tag="ACG827_000088"
     CDS             complement(16162..17862)
                     /locus_tag="ACG827_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0482264.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18129..18902
                     /locus_tag="ACG827_000089"
     CDS             18129..18902
                     /locus_tag="ACG827_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482265.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(18983..19552)
                     /gene="thiT"
                     /locus_tag="ACG827_000090"
     CDS             complement(18983..19552)
                     /gene="thiT"
                     /locus_tag="ACG827_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0482266.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(19618..19720)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19726..20697)
                     /locus_tag="ACG827_000091"
     CDS             complement(19726..20697)
                     /locus_tag="ACG827_000091"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0482267.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(20791..22164)
                     /gene="glmU"
                     /locus_tag="ACG827_000092"
     CDS             complement(20791..22164)
                     /gene="glmU"
                     /locus_tag="ACG827_000092"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0482268.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(22667..23485)
                     /gene="purR"
                     /locus_tag="ACG827_000093"
     CDS             complement(22667..23485)
                     /gene="purR"
                     /locus_tag="ACG827_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0482269.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(23652..24476)
                     /locus_tag="ACG827_000094"
     CDS             complement(23652..24476)
                     /locus_tag="ACG827_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0482270.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(24449..25129)
                     /locus_tag="ACG827_000095"
     CDS             complement(24449..25129)
                     /locus_tag="ACG827_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482271.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25152..26090)
                     /locus_tag="ACG827_000096"
     CDS             complement(25152..26090)
                     /locus_tag="ACG827_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0482272.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26335..27186)
                     /gene="ispE"
                     /locus_tag="ACG827_000097"
     CDS             complement(26335..27186)
                     /gene="ispE"
                     /locus_tag="ACG827_000097"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0482273.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27340..29913)
                     /gene="mprF"
                     /locus_tag="ACG827_000098"
     CDS             complement(27340..29913)
                     /gene="mprF"
                     /locus_tag="ACG827_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0482274.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30054..30860
                     /gene="proC"
                     /locus_tag="ACG827_000099"
     CDS             30054..30860
                     /gene="proC"
                     /locus_tag="ACG827_000099"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0482275.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(30939..32495)
                     /gene="rny"
                     /locus_tag="ACG827_000100"
     CDS             complement(30939..32495)
                     /gene="rny"
                     /locus_tag="ACG827_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0482276.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(32906..33955)
                     /gene="recA"
                     /locus_tag="ACG827_000101"
     CDS             complement(32906..33955)
                     /gene="recA"
                     /locus_tag="ACG827_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0482277.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34064..35305)
                     /locus_tag="ACG827_000102"
     CDS             complement(34064..35305)
                     /locus_tag="ACG827_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0482278.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(35545..36123)
                     /gene="pgsA"
                     /locus_tag="ACG827_000103"
     CDS             complement(35545..36123)
                     /gene="pgsA"
                     /locus_tag="ACG827_000103"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0482279.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36332..37255)
                     /locus_tag="ACG827_000104"
     CDS             complement(36332..37255)
                     /locus_tag="ACG827_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0482280.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(37994..39280)
                     /locus_tag="ACG827_000105"
     CDS             complement(37994..39280)
                     /locus_tag="ACG827_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0482281.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39273..40541)
                     /locus_tag="ACG827_000106"
     CDS             complement(39273..40541)
                     /locus_tag="ACG827_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0482282.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            40793..41254
                     /gene="mscL"
                     /locus_tag="ACG827_000107"
     CDS             40793..41254
                     /gene="mscL"
                     /locus_tag="ACG827_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0482283.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41313..41759)
                     /locus_tag="ACG827_000108"
     CDS             complement(41313..41759)
                     /locus_tag="ACG827_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0482284.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(41852..42097)
                     /locus_tag="ACG827_000109"
     CDS             complement(41852..42097)
                     /locus_tag="ACG827_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0482285.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42212..42790)
                     /locus_tag="ACG827_000110"
     CDS             complement(42212..42790)
                     /locus_tag="ACG827_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0482286.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(42867..43397)
                     /locus_tag="ACG827_000111"
     CDS             complement(42867..43397)
                     /locus_tag="ACG827_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0482287.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(43401..44261)
                     /gene="rlmB"
                     /locus_tag="ACG827_000112"
     CDS             complement(43401..44261)
                     /gene="rlmB"
                     /locus_tag="ACG827_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0482288.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44254..44649)
                     /locus_tag="ACG827_000113"
     CDS             complement(44254..44649)
                     /locus_tag="ACG827_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0482289.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(44646..46058)
                     /gene="cysS"
                     /locus_tag="ACG827_000114"
     CDS             complement(44646..46058)
                     /gene="cysS"
                     /locus_tag="ACG827_000114"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0482290.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46055..46591)
                     /gene="epsC"
                     /locus_tag="ACG827_000115"
     CDS             complement(46055..46591)
                     /gene="epsC"
                     /locus_tag="ACG827_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0482291.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(46677..46916)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(46913..48373)
                     /gene="gltX"
                     /locus_tag="ACG827_000116"
     CDS             complement(46913..48373)
                     /gene="gltX"
                     /locus_tag="ACG827_000116"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0482292.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(48543..49700)
                     /locus_tag="ACG827_000117"
     CDS             complement(48543..49700)
                     /locus_tag="ACG827_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0482293.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(49734..51104)
                     /gene="radA"
                     /locus_tag="ACG827_000118"
     CDS             complement(49734..51104)
                     /gene="radA"
                     /locus_tag="ACG827_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0482294.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51167..51694)
                     /locus_tag="ACG827_000119"
     CDS             complement(51167..51694)
                     /locus_tag="ACG827_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0482295.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            51924..52730
                     /gene="proB"
                     /locus_tag="ACG827_000120"
     CDS             51924..52730
                     /gene="proB"
                     /locus_tag="ACG827_000120"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0482296.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            52727..53974
                     /locus_tag="ACG827_000121"
     CDS             52727..53974
                     /locus_tag="ACG827_000121"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0482297.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54050..54394
                     /locus_tag="ACG827_000122"
     CDS             54050..54394
                     /locus_tag="ACG827_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482298.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(54511..54837)
                     /locus_tag="ACG827_000123"
     CDS             complement(54511..54837)
                     /locus_tag="ACG827_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482299.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(55452..56654)
                     /locus_tag="ACG827_000124"
     CDS             complement(55452..56654)
                     /locus_tag="ACG827_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0482300.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            56771..57286
                     /locus_tag="ACG827_000125"
     CDS             56771..57286
                     /locus_tag="ACG827_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0482301.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(57485..57931)
                     /locus_tag="ACG827_000126"
     CDS             complement(57485..57931)
                     /locus_tag="ACG827_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0482302.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(57978..59456)
                     /gene="msr(C)"
                     /locus_tag="ACG827_000127"
     CDS             complement(57978..59456)
                     /gene="msr(C)"
                     /locus_tag="ACG827_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0482303.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(59810..60439)
                     /locus_tag="ACG827_000128"
     CDS             complement(59810..60439)
                     /locus_tag="ACG827_000128"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0482304.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(60423..60887)
                     /locus_tag="ACG827_000129"
     CDS             complement(60423..60887)
                     /locus_tag="ACG827_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0482305.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(61374..62642)
                     /locus_tag="ACG827_000130"
     CDS             complement(61374..62642)
                     /locus_tag="ACG827_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0482306.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(62873..63610)
                     /locus_tag="ACG827_000131"
     CDS             complement(62873..63610)
                     /locus_tag="ACG827_000131"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0482307.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(63677..64246)
                     /locus_tag="ACG827_000132"
     CDS             complement(63677..64246)
                     /locus_tag="ACG827_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0482308.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            64374..65246
                     /locus_tag="ACG827_000133"
     CDS             64374..65246
                     /locus_tag="ACG827_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0482309.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            65588..66460
                     /locus_tag="ACG827_000134"
     CDS             65588..66460
                     /locus_tag="ACG827_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0482310.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            66457..68415
                     /gene="nagE"
                     /locus_tag="ACG827_000135"
     CDS             66457..68415
                     /gene="nagE"
                     /locus_tag="ACG827_000135"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0482311.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(68747..69598)
                     /locus_tag="ACG827_000136"
     CDS             complement(68747..69598)
                     /locus_tag="ACG827_000136"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0482312.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            69721..70188
                     /locus_tag="ACG827_000137"
     CDS             69721..70188
                     /locus_tag="ACG827_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0482313.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(70451..70933)
                     /locus_tag="ACG827_000138"
     CDS             complement(70451..70933)
                     /locus_tag="ACG827_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0482314.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(71033..71896)
                     /locus_tag="ACG827_000139"
     CDS             complement(71033..71896)
                     /locus_tag="ACG827_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0482315.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            72494..73369
                     /locus_tag="ACG827_000140"
     CDS             72494..73369
                     /locus_tag="ACG827_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0482316.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(73514..73666)
                     /locus_tag="ACG827_000141"
                     /pseudo
     CDS             complement(73514..73666)
                     /locus_tag="ACG827_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(73934..75265)
                     /locus_tag="ACG827_000142"
     CDS             complement(73934..75265)
                     /locus_tag="ACG827_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0482317.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT        21341 a        16047 c        13403 g        24559 t
ORIGIN      
        1 ttcttctgtt gaagattcag gtgttgttga ttcttctgtt gctgaagatt gtgtagctga
       61 tgatgattct tcagtcattg aagattgtgt agctgatgat tcttctgttg ctgagctttg
      121 tgcagtagat gaagcttgag tagcttgtgc ttcagcctct gcttgttcgc gtgcttcttt
      181 ttcagctttt tcttgtgcag cttgttgacg agcttgttct gctagacgtt gttgttcacg
      241 aatacgagct tgctctgctt cagcttctgc tttttgacgg ttcaagtcag ctttcttgtc
      301 ttcagcagtt gcttgttcag cagctaatga agttttcagt acgttcaaat cagcttgttt
      361 agataaaaga tcgccttttt gagattccaa agcagcttgg ttttcagcta attcttttag
      421 tttagcatcg ttttcagctt ttttgtcttc tacagctttt ttatcttgtt tttgttgttc
      481 catcaagtcg ttgtttgctt ttaccatcgt tgtcattgct tgaacgcgtc caatagcatc
      541 tgctaatgaa tcagcattca atacagcatc gatgtagctt gagcttgtgt tgcttacttg
      601 tgcttcgcgt gcttgttttt ggattgtatc ttcacgtttt tcaatacgtt cttgtaaatc
      661 tgcaatgtct tttactaatt gtgcagattc ttgacgcaat gtatcttgtt ttgctaatag
      721 gtcttgtgct tgtgtgttga tttcagaaac ttggctttct aaggcatcga tttgtgattg
      781 tgcatctgct tgttgatttt tcaagtctgc aatcttttga tcttgctgtt gtatctgaga
      841 atcaaaatcg tccgccgcgg cagcgatcgg tgatgctacg gcagtcaagg tcattgaaca
      901 taccattact gctgatatta aactcttttt cactcttcat tcctccgact ggcttaattt
      961 aataaatatc taaatatatt taagtctttc ttaacgatat aaaaattgta ccatagcagt
     1021 gtgtcaacga tataatagtt atgttacaaa aatgtttcaa aacaactttt aaaaatgaca
     1081 aaactgttcg tttcagaaaa tgatcatgaa aaagccgcgt taagtctagc atttcttaag
     1141 ataacaacga ttctttttcg ctttcgacct aaaaataaaa aaagaaatta ttcatcactg
     1201 aataatttct taaagggaaa aatgaataat acaaatataa tcatgttcaa cagcagtgta
     1261 ggtcctaaaa accttgtaat aaaatacgtg ttatttacta ctgctaattt aaaaatcaac
     1321 tggaccacca tcgtaaacaa ttcataaccc gtgacaaaga tgatcatact gaaaaattca
     1381 gtaaagatgt tgacatggat aacgtctttc attacataca tcaaccatac aattaaaggg
     1441 agagctactg catagatacc gataacgcca atatagtaag cgtcatagat agccccgaga
     1501 acaatcgtgg taatcagtaa ataacgtttt tcaaacgcca tactgcaaca taataatgcc
     1561 aatatcagaa agtgggcatt tgacatatag gtgcctttcg accactcccc tagcattctt
     1621 gttaaatgac catctatcaa catcaaaaag aacaaaacga ttggcagata gtacttcatt
     1681 gtttctttct tcaacatgga ctaatcctcc gctagccgtt ggacaatcgt cacaactgac
     1741 acgtcataca tttctgcata aggtttcaca taaacttccc gatccagccc ataactatct
     1801 ggtttcgttt taataaccgt accgatcggc aagttagctg gagaattccc tccaagaccg
     1861 gatgtctgga caacatcccc ttctttgata tccgtatcac cagttaattg ggtcaccact
     1921 aaggcatgga gcttttcatc atagttttta agcaaaccaa acgcttcgcc attagccgaa
     1981 gatacccgta ctggaaaatg attggagctt tcattagagg atgacagtaa ttcgatttta
     2041 gacgaagccg tattgacctc gatcactcgg ccaatcaatc ctttttgcga catgactgcc
     2101 atatttgctt cgataccgtc tttagaaccc ttgtctacga tcagcatgtc ctgccaactg
     2161 tcaggcgaac gagtaatgac atttgccgtt actttttcgt aactagtcag cgtctgattc
     2221 aactcaagtt cttttttcaa cgcttcgatt tctctctcgt aattcttgtt ttgctgtgaa
     2281 acgtcatcat agctatcgat tttttctttt agccgctcat tttcggcata agttgaaaaa
     2341 agattatgga cagaatctac tccatttcgt acccaccgta ctggggcaga gattgtttta
     2401 tcaaccattg caacactgtc attaacgatg gattggaaga aattgggctt tccttcattc
     2461 gctcgctgcg cagcggtcac acttagtatc gtcaccacga tgataacaat aaccaaggta
     2521 ataataatat ttttattagg attgaacttc ttcacaaaga cacctcgaat taattagact
     2581 cttgtgttca attttatcac taaatgccgt gaaacaaaag gtaagttgct cctcttaagc
     2641 ttttttttag caaataatag attctgtcat ttttagcctg aaagacaaat ttttttattt
     2701 gtttatggat caatctttgc tttgaataac tgcaatcata ccatctgaaa aagaaaattc
     2761 tgcggtatct gaaagaaatt cgttacttgc ataagatgtc ggatattcta cttctttatt
     2821 ttccaataca tacttgctgc cttttaatgt aagattcgag acgggagtca agcagcaata
     2881 agctaaatac ttcatatctg gtatctgtct tactatatgc tctccaggca aataataaga
     2941 caaataattt tgacaatctc tgataataag ctgatgggca aagggtttga agcgaggttc
     3001 catacctaac cagagattgg ctaaaagatg atccaatctt ccgcccgtag ctccaatcaa
     3061 agtgacttcc gcttgcgggt aacgttcaaa tgtttttaag atacctaact gtgtatctgt
     3121 atcgtctttt tctgcagggg aacgggtata ctctttggct tttgcaaata cttccttctg
     3181 ctcttcgttc gacaatgaat caaaatcacc cactgccaga tctactgggt agcctgcttg
     3241 aagcaggtac agcgcaccac ggtctatccc gacataggca tcgtatttgg acagcagaga
     3301 ctgcggccag ttttgcggat tacctccagc agctaataaa attttcattt agtctaatgc
     3361 ctcttttaat cgttcgattt gtccgactgg attttcggct ccatagatat atgatcctgc
     3421 aacgaagaca tccgcccctg catctttaca gatttttgct gtttctggaa cgattccgcc
     3481 atctacttcg attgcataag tccaattgtt ctctctacgt agatcagcga gttccgtaat
     3541 tttttctact gtttcttcaa taaagctttg tccgccgaac ccaggattga cagtcatgac
     3601 tagtacttga tcagccaaag gcaaaacatg tttgatcatg gagactggtg ttcctggatt
     3661 gataactact ccagctttaa ctcctaagct ttttgtcatt tgaagtgccc gatgaatatg
     3721 aggagttgct tcgacgtgga cagtaatgat atctgctcca gcttttgcaa atgctggaat
     3781 gtagttttct ggatcaacga tcatcagatg gacgtctaac ggtaatttag taacaggacg
     3841 gattgcctga acaatatttg gccctaatgt gatatttggg acaaatcggc catccattac
     3901 atctacgtgg atgtaatctg cgcctccttt ttccactaac tcaatgtctc tttgtaaatt
     3961 ggcaaaatct gcactcaaaa tggatggtgc tatcttcatt cttgtttcct cctattttcg
     4021 tttaccttct ttttttgcat acattggttt tcttttttcg atttcttgta agaattgcaa
     4081 ataattgtcg taacgtgtct gtgcgatacc acctgtttct actctatgtt ttacttcgca
     4141 gccaggttct ttagcatgca tgcactctct aaatttgcaa tggacagaag cttctacaaa
     4201 ttcaggaaat tgtctcggca gttctcctgc ttcaattgtc aaaaagtcaa tcgagctaaa
     4261 tcctggtgta tctgctacta acccgccata gataggaatc agttccacat gacgtgttgt
     4321 atgccgtcct cttccaagtg catcagaaat ctctcctgtt gctaaattca gttccggtga
     4381 tatttgattc agtagcgtcg attttcctgc tccagactga cccataaaga ccgatagccg
     4441 ttctgggaag taacggatca gttctgtcgt atcacccgta tgattgggta caatcaccgg
     4501 atagcctatt ttagtatagg tttgcttgat ctcttcgatt ttttctgggt cgtctgacaa
     4561 gtcggtcttt gtcaaaaaaa taatcggttc gatcgactcg tattccaagg taactagaaa
     4621 gcggtcaagt agattataag aaaatgcagg ctctacgata cttgtgacaa tgattccaag
     4681 atcaacattt gtgactggtg ggcgaactaa ttgatttttt ctcggcaaga tttccagaag
     4741 atagccgtct gtttgatttt cactctcaaa gatcacctga tctccgacaa gcggcgtgat
     4801 tttccgattc cggaaatttc ctcttcctct tgtttgatag gtctctccgt ttgcgtatac
     4861 atagtaaaaa ccgctgattg cttttctgat ttgtcctttg ataagtgcca cctccgtttt
     4921 tgtttatttt accatacgaa gaggaaagta ggtgttattc catgcaaact tctcaaagta
     4981 tatggcttga aaattgatca aaagagatag aaacacaaaa aagagaatgc aacaaatcaa
     5041 tgtcgcattc ccttctgata tattttattc agcacttatt ttgaactttc tgaggtgcta
     5101 gctgatgaac ttgtggagct ggaagattct tcttttgaag agttgcttgt gctactggag
     5161 ctacttgagc tgctagaact gtctgaagag tctttttcag aactagaaga cgctggatct
     5221 ggtcctttag agtaaattac gttcacaacc attcctttgg taattgtcgt tcctgcccct
     5281 ggtactgtac gaatcacttt atctttatcc accgtgtttg aatattcgga agtctcatgc
     5341 ccgatatatt ctgcaccgac actagctaaa taactttgtg cttcgctttt tgtatagccg
     5401 gagatatcat tcaatttagg ctctgttcct tggcttacga caaaattgat gtaagtttct
     5461 tctgctacaa cttcacccga ggctggatcc tgactgatga tcgaacctga tggtacagtg
     5521 tcgctatagt cataagtagc actaatatta ttgatattga atccttgtgt aacaagccaa
     5581 ttacgaacag tttcgtagtt ttctcctaca tgactactca aagtcactgt ttctggtcct
     5641 ttgctgactg tgaaagtaat cgtgtctgtt ttaggatcga cttctgtatc ttctgatgga
     5701 gattgcgaaa tgatcattcc gctttctacc tctgaactga attcctcttc tttttgaatc
     5761 aagtcagcag aaaaccctaa ttctttcaaa cttgcttcgg catcttcata acttttacca
     5821 ctgtaatcat ctaatttgat ttttttcgtg ccgctactaa tatacagagt gacttcgcgt
     5881 ttttgcttga ctgtcgttcc cgctgccggg tctgtcttca ctacattacc ttcttcgatt
     5941 ttgtcatctg caatttcttc tgtttttgtt gctacttcta atttcgcttt tgcgagtgtt
     6001 tctctagcag aagcttcaga aagatcagaa acatcaggaa caacaacttc tcctcgcccg
     6061 ccagaagcaa agaatacagc tgttccgatt cctaaaacag caagaatagc taataggatc
     6121 agccaccatt ttttctgtcc tttcttttta ggatcaggca gaacttcttc ctcctctggt
     6181 aaggtttcgt tttctggctt gtcttttggc agaggcatag atttgaatga atcaggcatt
     6241 ggtgcatctt ccgtaagggg tgtgataact tttgtttcat tatccatcac atgaggctgc
     6301 catttttctt cgtttgcacg agctggtgat aatactgtag aaagatcatc agacatttcc
     6361 tcggctgact tataacggtc tgcaggttct ttagccgttg cccgtaaaat cacgttttcc
     6421 agtgcttgtg gcacatttgg atcaagtgct tttatagatg gaagatcatc ttgaaaatgt
     6481 tttaaagcaa ttgttacagc agattcacca tcaaacggta cgctacccgt caacatctca
     6541 taaaggatga tccctaaagc ataaatatct gactgcttcg ttgccatact tcctctggcc
     6601 tgctcagggg acagataatg aacagatcct agcatcgtat tcgtctgcgt gatcgatgtt
     6661 tcagataaag cgatagcaat cccgaaatcc gtgattttta caacaccttc attatcgatc
     6721 agtacgtttt ggggtttcaa atctcggtgg atgatttgat ggttatgagc taaagaaata
     6781 gcagaaagga tctgctgcat aatatttact gctgtttcat aaggaacagg ataatgtgtt
     6841 tggatatatc gtttcaagtc cattcctttg acgtattcca tcaccagata ctgcatgttg
     6901 tcttcttcac caacatcgta aacactaaca atgtttggat gaacgagttc tgttgctgct
     6961 aaagcttctc tctgaaatcg tcgaatcgca gtctggtcat tttgaaagtc aaatcgtaag
     7021 actttaactg ctacatcacg atccaagatt aaatcgtgcg ccaaaaatac atttgccata
     7081 ccaccgctac cgatatttcc agtgatttga taacggccat ttaatttttt gcccaactcg
     7141 atcatacact gtcctcctta tattcgataa gaagaacggt gatattatcc gctccgcctg
     7201 cttcattggc acgatcaatc agctcagtta ctttatgaga caatgggcct tcttgattga
     7261 tgatcgttcc tatttcttct tcagacagca tgttcgtcaa gccatctgaa cataacagca
     7321 gtcgatcttt tagttgccaa agatgagtag aaacatctac ttcgactgtt ccaggcatgc
     7381 cgacagaacg tgttaaaata tttttccttg gatggttcac tgccatttct tcgctgattt
     7441 ctcctgattt gactagttca ttgactaaag aatgatcctc cgtcaattgc atgattttat
     7501 cttcacgaat caaataagct ctgctatccc caacattggc aatcgtgaac cagtcatctg
     7561 acagagcggc cgctacgatt gttgttccca taccgttata ttccggctgt tcttgtccac
     7621 gctgataaat cagcatattt tcttcttgaa tcgattgaat gaaccattgg gcgatttttt
     7681 catcgtcaac agcttcttgt ttttcccaag cttcgcctag attcgttacc gccatctgac
     7741 tggcaatgtc tccggcacga tgaccaccca tcccgtctgc taatacagca agcttgagac
     7801 ctgaacggct ggtaaataca ctggcatagt cttgatttgt gttacgtcgt cttcctacat
     7861 ctgattgata ttcaatctgc attttttcac ctcattactt tttgcgcaaa caacaaatga
     7921 aaaatccatc tgtgtaaaac tgatgcggat acaaagtcaa cattttctct tcgattgatg
     7981 attgtagcat aggattggtc aaaacatcaa tcctctcaaa atcagggtgt ctttccaaga
     8041 aagcagaaac aacgtcttgg ttttcttctt ttaagatcgt acaagtgcta taagtcagaa
     8101 taccggaaga ctttaaagtt ggtgcgcagc tttctaaaat ttccaattgg atttttggta
     8161 aattcgctaa ttcatccggc tgttttttat aacgaatatc tggttttcgc cgaagcagcc
     8221 caagacctga acaaggcgcg tctactaaaa tccgatcaaa ctgctctgct tcgaactgtt
     8281 tactgacttc tctagcatcc atcttttgag cgtagacagc agaagagaca cctagacgtg
     8341 ctgcattatc ttcaatgagt ttgactttgt gttcgtgtac atctaaagcg accacttctc
     8401 caccgcatga cggatctaaa aaagaagcaa tatgcgtcgt cttacctccg ggtgctgcac
     8461 aggcatctaa gacatgatgt tcaggctcga tttgcataga cggcgctaca agcatcgaac
     8521 tttcatcttg gatcgtcatg acgccatcgt ggaacaatga gctagaagct aaatgccctt
     8581 ttttcgctac tataccgtat ggactgactt ggcttttttc agctgtgatc tcttcttctt
     8641 gcaaaacagt gatggcctga tctctggaaa gtcggcgtgt atctactcgg gcactagcat
     8701 ggctaggctg atatagcgat aaaccgagct tttcagtttc ttcgtatccg atttgatcca
     8761 ccagtcgttt cgtcatccat agaggcaggc tgattttgac agaaagtctt tcgataggat
     8821 cagcaatgcc ttgcagatct ggtgctcctt gacgctgata attcctcaag acaccattta
     8881 caaatttccc tgtcccagga tttcctttta cttttgcgat ttcgaccgcc tcgtttaaaa
     8941 tagcatgtgc agggacttta tctaaaaatt ctaattggta tacagaaaga taaagaagca
     9001 tcttgaccca gtcatccact ttttttgctt tagcgatcaa tggtgcaacg taaaattcca
     9061 atagtcgctg atggctaatc gtaccataga tcaattctgt aaataaaccc acatcttttt
     9121 gatttagctc gctttgtttg atcatctcat tcaacaaaag attcgagtag gcgccaccct
     9181 tgttcactcg ctctaatgtc agtaatgcca tatatcgtac tgattttttc atttttttag
     9241 ggattttttt cattatttca cctgctgacc aatctgaact tgctgaccgc ttccgttaag
     9301 gaaatcatgt acggcctgtt ttcctttacc agctggctgt aattcattga tggctaagac
     9361 ggtctgttta ccgcaagcaa tccatagatt ttttttgtct ttccggatga tcgtaccagg
     9421 tgctttgtct gtagtttctg ccaatacttc aaccgataat aatttccagc gtatgccctc
     9481 ataggtcgtg aatgcaattg gccaaggacg cattccgcgt acttggttgt caatttcttc
     9541 tgcagtttta tgccaatcga ttgcttcttg ttcccgtgtg atgtttggcg aaaaagttgc
     9601 ttctgactcg tcttgaggta ctggtttttg tccatccaaa atattgggca atgtttctag
     9661 caatagcttt cttcctaaaa ggctaagctt ttcaaacatc gtccctacat catcttgttt
     9721 agtgattggt atgctttctt gcgcatagat tccgcctgca tccatttttt tgatcatttc
     9781 catgatcgtt acaccagttt ctttctcccc attcatgatg gcataatgaa caggcgcacc
     9841 tccccgatat ttaggaagta aagaagcatg gacattgatt gcccctaatt ttggtacttg
     9901 caataacttt tcaggcaaga attggccaaa tgccgcagtg acgatcacat caggcaccaa
     9961 ttcaatgatt tcttccattt ctggcgaacc ggagattttc tctggctgca gcacacggat
    10021 accgtgcttc agagccgctt tttttaccgg tgtcggcgta attactcgtt tccgcccaac
    10081 tggacggtcg ggctgtgtta ccactgcctg gatctcgtaa cctgattcta tcagcccttc
    10141 aagaatcggc acagaaaaag caggtgtgcc cataaatact agcttagtca tcaagatgtt
    10201 cctccatata ttcttcaagt tcttcttccg ggatctgctg tatcattttc tcaatgaaca
    10261 agataccgtc taagtgatcg atttcatgct ggaaggcacg ggctaaatag ccaaaagccg
    10321 tcacttctat ctcttctccg tctcgatcat aataacgcac ggtcacttca tctgctcgct
    10381 tgaccgttcc atagacatgc ggaatactga gacaaccttc tacgtcaagg ctttcacctt
    10441 ttgcttcgat gatttctggg ttaatcagtt caaatttctc gccttcatct acttcgatca
    10501 cagcaattct cttgttttgt cctacttgag gagcagcgat ccctattccg tcattcgcaa
    10561 tcattgtctc atataaattg tctaataaag cgattgtctc gtccgtgatt acatcgattg
    10621 gctgagcagt tctttttaat ttatcattag ggtggatcaa tataggatag cgcataattt
    10681 ggccctttct taaataaagt ttaatggttc gttgtcaatt gccacaaaaa gaccttgtcg
    10741 ctgttctttc tggcttccat tcaaaatttc atccaaaaca tgaggaagat tcggttcatg
    10801 tttatatttg atgattagtt gataataata tcggtttttc actctcaaaa tcatgctggg
    10861 cgttggccct aatagcaagc tttgagggct taacacttct tttagctgat tggcaatctg
    10921 aaagattttc tttgccgcta cttgttcttc aggatggctg gcggttattt tcacagtaaa
    10981 gtaataagga ggatagcctc cacgatgccg tacatgcatt tcctgctgat aaaactgctc
    11041 ataattttgt tttttagcca attctatggc gtaatgttga ggattgaatg tttggatcac
    11101 gacttcgcct gctttttccg ctcgccctgc tcgtcctgaa acttgggtaa gcaattggaa
    11161 tgtatgctca ctagaacgaa aatccggtaa attcagggag gtatcagcat tcaacacccc
    11221 aactaatgtc acttctggaa agtccaaacc tttagcaatc atttgtgtcc ctaataatat
    11281 atctgcttct tttgcaccga atttttgtaa aatctgttcg tgtgcccctt ttcttcgagt
    11341 ggtatctaca tccatacgca aaatcctagc ttgcggataa agttctcgga gttcctcttc
    11401 tactttttga gtacctgttc catagtagcg gattttattg ccgccacaat taggacagcg
    11461 attgggaata ggttcttcat gtccgcaata atggcagcgc attgaacgtg tatccatatg
    11521 cagtgtaaga gagatatcgc aattaggaca tggaagaaca aatccacagt ccctacacat
    11581 cacaaaagac gaatatcctc gacgattgag cagtaggaca gtttgttctt ttttgtctaa
    11641 tcgattttgt attttttcct gcaaattagc agaaaaagta gaggtccgat gattctgaaa
    11701 ctcttcacgc atatctacga tctcaatagc tggaagctgt gcagctgctt tggctcggtg
    11761 gtttaactgc aataactgat aaacgccttt ttgagctcgt gctcttgatt ctaaagaggg
    11821 cgtcgcgctt cctagaacta ttgggcaatg atgatattta ctgcgccaaa ttgctagatc
    11881 tctcgcatga taacgcggtg tctcatcttg tttgtaactt gattcatgtt cttcatcgat
    11941 aatgatcagc ccgatatttt tcagcggggc aaaaatagca gatcgtgcgc ctactactac
    12001 ttgcgcttct tcccgctcaa tcttgcgcca ttcatcatat ttttcacctt gggaaagtcc
    12061 gctatgtaag accgcaactg attctccgaa gcgtcctttg aatcgctcga ccatttgcgg
    12121 agtcaatgcg atttcaggca ctaacataat agccgtttta ttttctgaca aaacatcagc
    12181 gattgcctga agatatactt ctgttttccc gctgccagtg attccctcca gcaaaaagac
    12241 ctgatctttt tgctgctgac ctgctgtgat gatttgttcg acagcatttt tttgttctgc
    12301 attcagttcc aatgccgtcg tttggtcaaa tactcggtct ttataagggt cgcgataacg
    12361 ttctgattct ataaactcca gccagccctt ttcttttcct tgattcaaga cagcagtact
    12421 gattccttta tttttgaaat aagcaattgg ttcttcttgg ctcagttcag ataaacacaa
    12481 aagaagttct ttttgtttct tggcattttg ccgtaatttt gcccattctt cttcgatttg
    12541 cttttcagtt aacaaagaac ggaccaaacg aatcgttttt actttatttc gtgtatggac
    12601 ttcatatttg atatctacta attgctcttg gcgccagcga atcaattgag aaaggctgcc
    12661 ttcttcttct gcttccttcc aggaaatcac ttctcgctga tcaaacaata cctgaacttc
    12721 tggtgtgtct gccaaaggat agatcaattt atcatattct gctttcatca cactcggaag
    12781 catcgtttgt agacaagtga ttttgaatgc gtaagtaatt tttttcatat aatcagctaa
    12841 tgaaagtaat tcttcattga ccaccggagc aaggtctagc aaacgaataa ctgccttcaa
    12901 agatggatta gctgattctt ctgatttttg gatagccata acaaatcctt ggacatgacg
    12961 attgccatta ccaaaaggga cttctactcg catcccgact tgcaaagctg tttccatttc
    13021 agagggaacc aaataagtga atggctgatc ggtctgcata gtaggtacgt ccacaatgac
    13081 ttcagctgtt aatgtcatgg ttcgtttcct cctttttttg tcttttctta tcaagcgaca
    13141 aaaagttcct tgcctatttt actagagatc gatggatttg tcttatcttc tgccaaatcc
    13201 taacaaaaaa ttaacagcag ataagaaaaa aaagaactgt gtcaaaaagt tcagacacag
    13261 ccttttctac cagacaataa gtggcgaatg tttgaaaagg aaacagctgg gtttgctttt
    13321 ctttttagat gttcgctcta tttttcttta ttacattttt tgctcttcac ggatacgtga
    13381 ttccaattcg tgttgttcac gctctttttg tgcttgacgt tcctcttctt ccatcttcaa
    13441 acgttcacgt tttaattcag gatgtggatc attgactaca tttcctgcat cgatttcttc
    13501 taaagcttga ccgacgcttt ttactgaatc aaaagaatct agagtaggct gtgcacttgc
    13561 atccaattca tgcgcgcgtt tacttgctaa aatcactaga gaatattttg aatttacacg
    13621 atctaatagt gagtcaattg aaggttttaa catcataaga ctacatctcc tttaacattt
    13681 taatatattt accgattacg cgatctactc gaaaatgttc actggcaata atgtttttga
    13741 ttcgttcaac tgctagcggc acttcatcat ttacaacagc atagtcgtaa agagccatca
    13801 tttcgatttc ttcttttgca actttcattc gtttttcgat tacttcatct gcatctgttc
    13861 ctcggcctac gatccgtgat tttaattccg ctaaatctgg cggagtcaaa aagatgaata
    13921 caccatcggg cactttttct ttgacttgtt ttgctccttg gacttcaatc tccaagaaaa
    13981 catcttttcc ttcatccaat gtcttgttga catatgacaa aggagttcca taataattcc
    14041 cgacatattc tgcgtattcc agcatctctc cagcttcgat cattgattca aactcttctt
    14101 tgcttcggaa gaaataatca acgccttcga cctccccttc acgcatttta cgggtagtca
    14161 ttgagatcga atactgaaaa tcattatcct cactctcaaa aattgctttt cgtactgtgc
    14221 cttttcctac accggatgga ccagacaaca cgattaataa tccacgctct gacatgacga
    14281 cgtcctttca actagtcttt ttgtacttat cgtttatttt gcactttttt tctgcaactt
    14341 tcaaggttat ctttaattat agctagaaat tggtaaaaaa gctaccctgt accaagaggt
    14401 cgtaaaaaag ctgtcctgca tcaagtaata agaacagcca aacataaaca gcttctcatg
    14461 tttttcgttt ggttggggtt attatcgcag atgcaagctt ttgaacgccg tgtataagag
    14521 gtcgtgaaga agccgattag tttcgagtat gaagaaaaat tttgtgaaaa tagtttctca
    14581 catttttgca taaatttggc ttaataccga gaaaatggtt tttgaacatc acttattcaa
    14641 aataaaaaaa gattgtgaca cgaattttgt cacaaccttt aaatgtattc ctagagcaaa
    14701 gaagctaagc gaagaaatcc aattctgttt atttattgta tttctttgtt cagcatcctt
    14761 gctaaaagcc catattggac aaagtattca ctacacccat gatctcatct tttcgagaac
    14821 gtgctttttc tgtcggatct tctacctctt ctgtgtcaat tcgaacaata aatttccgat
    14881 tcatcgtcac gtaagcaccg tcgttgatca aaataaacag ttcgttctca ttatagtctg
    14941 cacaggcatc tttccatacg tcaaagccgt ttttttcaga agggatatcc cgtttaacca
    15001 cagtttttcc acttaattcg tggaacgtta cgtcataata tttcattatc tctacctctt
    15061 tcgtcattca atattttgta cttgttctct gattttttct aagatcgtct tagactggac
    15121 gacttgattt ttaatttcga ttgcatttga tttggaaccg atcgtgttga tttcccgatt
    15181 catttcttgg atcaggaaat ctaattcccg acctacagga cattgtgctt cgataagttc
    15241 atctagtttt tgtacatgga tcgccagacg atccagttcc tcgtgaatat ctccgcgttc
    15301 caacaagatg gctagctccg tcaacaaccg ttgctcatcg actttcgctc caagaaattc
    15361 ctccagcttt ttttgatacc ttatttgaaa ttccttttca tagatttcta caaaactatc
    15421 gagttttttt agtacttctt tgaattcttc agtattcttt ttcaacacta aagctaaagc
    15481 ttgaccttct ttttctcgac tcgcctcaat agaatcaagt gcgcttttca atgtatctag
    15541 agctaactgt gccaattctt ccatgctctc ttccggtttt tcttctaccg ttacgaagtt
    15601 ttcgtccagc acgaagcgtt ccgccatccg actaattgac acttgatttt gactaccgta
    15661 tcgttgttga attccgtctg tcaattgagt gataagtgaa tcaatcaatt cccagttgat
    15721 gacgatctgt ttttcgtttt tgccaatata agctagattc acaaatactt cgattcgacc
    15781 gcggcttaac tgctgtttaa taagctgacg aaaatcgttt tcaacaaagt tcaggatttt
    15841 aggacttcgt atttgaacat ctagaaaacg ctggttgaca ctttttattt caatatctaa
    15901 ctgatacgct tttgtttcgc ggatggcttt gccaaaacca gtcatacttt tcattcgaca
    15961 gggtccttcc tcattacata ttttctattg ctttttgcag ttgttcaaac tcttcgttcg
    16021 tcaatgtcag gcctttcccc attttttcat ggtctggcgc ccaatctcga agatcgaatt
    16081 ttggcgggcg accattccag ctgactaggt tcagttcttt acgccacccc ttgttgtttt
    16141 ctgacaatat agcgatttct tctacgattt catatgaaaa ttcttgtgcc atggttgttc
    16201 ctccttgaga taatataaat ataggatcag taatgtgtca actggttgct taagcaattc
    16261 atgctgcaaa cgatccttgc gttttccttg tagcagataa tcaaaataaa cagttttcgt
    16321 gcgctcattt tgtatttttt gctggtattt tcgatacctt cgccattctt cagtacttaa
    16381 ttgaccaatt tgtgccaata aatcatccgg catacttttt atatcttgtt ggcagatcat
    16441 ttgaatcatc atgtctactt gatagatttc attgggcagt aaaaaatgtt tttttgctaa
    16501 aagttctagc atcgccaatc cgcatgtctc tggtacgacc ctgttcttat ataaattacg
    16561 cagtttttgc caaaatttta tgcttttgaa aaaagataat tctaactgaa tttcttttgt
    16621 taagtaagac aaaaatcctc gccagtatgt tcttgcaggt ttaacagaat gaaatgtata
    16681 ccagttccca tagtaatagc ctaatcgttt tttcatttcc agatagccta attgtaaatt
    16741 taattggttc ctttggctat caaaaagcag aaagctgcct agtgtccaca atgtttgaca
    16801 tttccaatgg ataaaaatgt ctggcatacg aatttttttc gctgggcctg gcccttgcac
    16861 atctacgaca aatgcctctg ttgctccttt gttaatcgca atatcaatag gtattttgtt
    16921 gcgataccca ccgtctgcat atttattttt tccgatcttt cgataagcca tagcgggata
    16981 aaatgaagca gaggcgagta tccaatctgg tatttcttct gtttttgtct gttggatatc
    17041 agtcactact tccgctagtt ctggcagttt agtagacacc gtatataaac gtattttggg
    17101 attatgtttg agtaaggaca aatccaaatt gtctttgacc atcttttcca atggagcaga
    17161 agaaaccccg ccttcaatta gtgctgtttt cgtcatttgg cgtctttctc gatagaagcg
    17221 ttcacgaagg tcttcaactg cagccatttc tggtaacgca agcacttgtc ttgtcgacag
    17281 ctttttccat aaattcacag cttttttcat atctccttgt aaaatcaatg cgccgttcaa
    17341 cgcacctacc gaggtacctg tgataatctc aaagttgata tcatgttcct tcagcgcctg
    17401 ccacacacca atctgataag caccgtgcgc tcctcctcca cctaaaacaa gtgctgtatg
    17461 atagaccagc tttttattta attgtgtggt tccttgatag ccgtttttat gaaaaatacg
    17521 caaccattct tctgatagag gagattgaaa taaaaaacaa cagttttctt taataaaaaa
    17581 acgagcgcaa gcttccatat accgaaaaaa ttttttccat tcaaaagttt caccttcaat
    17641 caacagatta ttaaaaataa catctcctct acttttagca atagtaaaat agacggtata
    17701 ttttttcgat actgctttaa aagcacattg cttttttgca gaacgccaaa aagcgtctct
    17761 actttcttga tgactttgaa aaaatggaag gggttgtttt gcttgctgga taggcgaata
    17821 cagcgacatt ttctgtgaaa agtaaacttt ttttatctcc atcgcgctct cctttttctt
    17881 caagtatacc agtacgtcat aaaaaggtca tgagtctcgt attcgacttt gtagattcca
    17941 taagaaaaca attcataaaa cctcctttaa ataaaaaaat acaaaaatca caaaaattaa
    18001 ttgaaaatga gagtgttttc aatgaaaacg tgattttgta atggaatttt aaaataaaag
    18061 aaacaagctt ttttaatgca aatgttgtaa aataaaagag attaagagga aataagagga
    18121 ggaacatgat gactgagtca gtttatgtac atattgatac cacaagtaat gctgttttaa
    18181 caaaagggct gactgctact gactttgcaa acagtatcgt gcattttcct caaaacttgc
    18241 tcttgcttga tccatctgct tctgctggag aatatgaatc ccatacaggg ctgaaagtaa
    18301 tccgtggtac agaaaatatt cagcgtttct tttcctcttc tcgtaacagg cgtggattcg
    18361 atgaattgaa gtggatcgac tttactgatt taacgatgct gaaagaattg acacctttag
    18421 aaatttcaga attattatat tttggtcata tgaaaacaca tcttcattcg ccgttttttt
    18481 ataaactaca gaataatttt gtatacttcg atcttaatga tcagctgaat cggatttatt
    18541 atcgctattt agatgaattt tataggattt ttgctagtaa attaagttcg atcttgtcag
    18601 ataagatcaa tgtaaaaaaa tcttttttca aaaaagaaac acctgttgaa aaaatcagtg
    18661 tggaattatt gaaagaaatg cgtaatttga tgcaggaagg gatcattttt tcttttgatc
    18721 aagttggttt gatcgatggt aaatatcgta tcccaatcta tgtgattgaa gattatattt
    18781 ggaaattaaa aggcacccgg tatcgagaag aggaaatcat cgctacttta atctactctg
    18841 tcaccgatcg acactggcaa ttggtagaag atgccgatct ttcttatctg ccattgaagt
    18901 agatctattt cttatcaaaa aataaaaagg aaacaagaaa ctaggacatg attctgtctc
    18961 aagtttcttg cttctttttt atttacagct cctgtcgttt atctggcaca aaaatcgaag
    19021 gagtcgtttg ttggatccct aataaaacga tgatcgttac aatagctgta ctcaacccgc
    19081 tggcaccatt cattacgaaa gaaaagagcc aaggattcat cccccatagt gcatacgctc
    19141 cccagaaaac cactcctgca ataaaatgcc aaaaatatct ggaaagtgcg ccaatcaatg
    19201 caccaaacac gatgcttttt ctaatttgtt tcgtattatc gtctacatag cctttttgga
    19261 catttgaagc catgagccct gctgcgcctg caaaagtata ggcaatcggg tactcaatca
    19321 acacttgaac gacacttaaa taataaactt gtcccaacgg aaaatgtagg atgccccaaa
    19381 tgaaacctgc cagcatccct gcctttgtcc ctcttcttaa cgcaaaaaga gtcaatggaa
    19441 tctgccctaa tgaaatagaa aaactgctgc cgatctggat cggaatcaac gacaacacca
    19501 tcgctaacgc tgcaacaatc gttccttcta cccaaactct attttttccc ataaaaaaac
    19561 ttcctccttt gaataaaaaa gtcacaaagg aggaagcagc tttcgaaaat agtcaaacag
    19621 ctgtttattc acaattccta cgctcgcata accgatacag gtacgaaggg tttagaaata
    19681 gattcaatct cagcccaatt ggactcccct ttgtgagttt atttattatt tattttttgt
    19741 ttcaaacaat ggactaactg gtttgttttc atggatacgt ttgattgcat ctcccattag
    19801 tccgccaaca cttacttcat cgattttgtc gattttacga tcttctggaa gatagatcga
    19861 gtctgtaaca actagacgct cgatagctga atcttctatt ctttgtaaag cagggccgga
    19921 taatactgga tgtgtacatg aagcatacac actagttgca ccagcttctt tcaaagcatt
    19981 ggcagccaat gtgatcgtac ctgctgtatc gatcatatca tcgatcagta cacaagtttt
    20041 gcctttgact tgtccaatga tattcattac ttctgcaaca tttgcttttg gacggcgttt
    20101 gtctatgatc gcaatcggag atttcaaaaa ttccgctaat ttacgtgccc gtgtgacacc
    20161 accatggtct ggtgaaacaa cgacaacatc atcgcctttg attccttttt ctaagaaata
    20221 attggcaata agtggcgcac ccatcaaatg atcgactgga atatcaaaga aaccttggat
    20281 ttggactgca tgaaggtcca aagtcagcat tcttgttgca ccagcttttt gaatcatgtt
    20341 tgctactaat ttcgctgtga ttggttcccg tgcacgtgct ttacggtctt gacgcgcata
    20401 gccataataa ggcatgacta cgttgattgt ttttgcactt gctcgtttca gtgcgtcaat
    20461 catgatcagc aactccatca agttatcatt gactgggctg ctcgttgatt gaataacata
    20521 gacatgcgat ccacgaatac tttcttcaat gtttacttga atttctccat cactaaattg
    20581 agtaacagat gattttccta actcgacgcc gactgctgct gcaattttct ctgctaatgg
    20641 gcgattggaa tttaacgcaa aaatttttaa tcttgggtca aagtaatgtt ttgacatggg
    20701 gacctccact ttctttttca caactctaga attctaccta ataaatagta gtcatatttt
    20761 ttaaataaat caagtgattt tgcaaaagtc ttagtttaga taagggagtt tttttgcata
    20821 tccttcttta ttgacttgtc ttgctctcgc aattgccagc gcatattctg gaatattgtc
    20881 tgtaattgtt gacccggcag caatcgaact gtttttagct acctcgacag gtccgatgat
    20941 attcgtgcta gaaccaataa agctgtggtc gccgacagtc gtatgatgtt tgttctttcc
    21001 atcatagttg acgaagacaa caccacatcc gacattgatt tcttctccca gcgtagcatc
    21061 accaacataa gtcaagtgac cgactttagt tcctttgccg atttgagcat ttttcacttc
    21121 cacaaagttt ccgatatgga cgttttcgcc aatttcagct tttggtcgta aatgtgcata
    21181 aggtcccaca tctgcatgtt tcttgacatg gctgctttca atgactgagt tttcaatgac
    21241 gacgtgatcc tcaatcctac tgtctacgat ttttgaatgt gacccgatga cacaatcaga
    21301 accaatgact gtatttcctt ggatttgtac acctgcttcg atcaatgtgt ctggaccgat
    21361 ttctacgcct gcatcgatgt atgtcgttgc tgaatcgaca aagcttacgc cattgaccat
    21421 atgcatctgg ttaattcgct ttctcatgat ctcatttgct ttagctaacg cgatccgatc
    21481 attgactccc attgattctt caaaatcatc cgtttgataa gcagcaactg tatggccttc
    21541 ttcttttaag atttcgatga tatctgttag atagtattca ccttgagcat tgtttgttcc
    21601 aactttgctt aacgcatcga acaaagcttc gttgtcaaaa cagtaagttc ctgtattgat
    21661 ttcttgtaca agagcttctt ctggtgttgc atctttttgt tccacgattt tttctacgat
    21721 cccaatatgg tcacggatga ttcgtccata tcctgttggg ttttctgctt gtgccgtcaa
    21781 gatcgttgca cttgcatttt ttccttggtg atattcaaaa agattgttca gcgtttcagt
    21841 agtcaataat ggtgtatcgc cactgataac taacgttgtt cctttttttc cttccaagaa
    21901 agaagcagct tgcaaaacag catgtcctgt tcccaattgt tctgcttgta acgcatattt
    21961 actgcgttct cctagttgtg ctttaacttg ttctgctccg tgtccaacga tcgtaatcac
    22021 ttcatctggt ttcgtttcac tgacccgatt aatgatatgt tctaccatag gtttaccaga
    22081 aacgggatgt aaaactttat ataattttga cttcatgcgg gtacctttcc ctgccgccaa
    22141 aataattgca taacgtgcgt ccaactgcgt cactcctaaa tatagtttgc tgatttcagt
    22201 ttatcccagt atacccattt tttcaacaaa acaagtgatt ttcttttatc ccagcgtttt
    22261 ctggataaaa aaatagatag accataacgg gcctatctat ttggtaaccg gtgctctcct
    22321 gccaactcct cggtcacaag tcacactact cataaaacat gaaaagctgt tttttgaaat
    22381 cgtttcttgt gacttagagt taaacggtag gcttacaacc tcttcattaa acgtttttca
    22441 atcagctgac cttcggtatt agctcaataa acggcaaaat atgaggagca atttttccgt
    22501 ttattgagct aatactcagg acaggacgct gatttcacaa cctctagata aacggtgttc
    22561 aaaagccagt ttctcggtat taagccaaat ttatgaaaaa atgtggaaag ccattttcac
    22621 aaaatttttc ttactactcg aaactaagcg gctttttcac aacctcttat tctgaaaaat
    22681 aatttcctgg cacaaccgta atatttttcg tttgagtatc tacatctttg acataaagca
    22741 gtgacgtata atcatcgatg gcgcgatgac cgttgaattt tgattcggca aatacagtga
    22801 tgccaacaag ttctgattcg aattcttcaa tcaagctctt cattccgttg actgttccgc
    22861 cgcctttcat gaaatcatcc actacaagga cacgtgaacc acgtttcaaa cttctcttag
    22921 acagttccat cttttcaatt ctttcagaag atccagacac atagttgaca cttactgttg
    22981 atccttctgt tatttttgag tcccggcgta caataacgaa tggcgcattc agataatagg
    23041 acacagcttg ggcaataggt acgccttttg tggctacagt cattacagca tcgatttttt
    23101 caccaaggta tttagaagca atgattcttc caacttgacg cagcagttca ggttctccta
    23161 acaaatcaga gagataaaca tatcctcctg gaagcagacg gtcttgttct gatagacgtt
    23221 ctgctagaga ttcaatatat tttttcgcct cttcatatga aatttcaggg atgaacaata
    23281 caccacctgc agcacccggg attgtttcca aggttccata ccctcgttct ttaaatgttt
    23341 tcttgatgat tcccaaatct tcactgatag aagatttagc agattcataa cgttcggcaa
    23401 agcttgttaa ggaaatcaat tcatgcgggt gattcaataa atactgtgtc atgtcaacta
    23461 gacgttcact gcgacgtact ttcacctatt ccaactcctt ttaagaaatt ttatcgtttt
    23521 acattatata agtttttctt caaaaattcg agcattttat gaaaaaataa gaaaatagtt
    23581 cggtatttat taaaataaaa agaagcctat aaaaaatttt tcgtttttat aaggcttctt
    23641 ttctttataa tttagttatt ttttcttctg aaggcaacca atattcgttt gatgatattt
    23701 acaatcagga acaaagcaat aaagactaat gtgattgttg ctcctggagg tgtatctaga
    23761 taaaacgagc tagttagtcc tgacagcatc cctagaaatc ctacacaaac actgatagca
    23821 atcacgacat tgaagctttt gcctaatcgc ataccgattg ctgcgggaag aaccatgatt
    23881 gcggatatca aaagtgcacc ggcaatcggg atcatcacag caatagccac accagtaatc
    23941 acattgaaca tcatcgacat caaatgaacg ggtagcccat ccacgtgggc ggtatcttca
    24001 tcaaaagtca gaacatacat cggccgtttg aataaaaaga acaatatacc gatggcaacg
    24061 aataagattc ctaaaaagat gacttgctcc catgtaatgg tcacgatcga accaaacaga
    24121 taggattgga tacttgttgc tgaattccct ccgctcaaat tcatcaatac taacgctagc
    24181 gccaaaccac cagccattaa aatggcaatg gagatttctg aataggttcg gtagatcgtt
    24241 cgcagatact ccaatatcac agctgctaat acaacgacta gcatggtcgt aagtgtagga
    24301 ttaaggttca aaaagaatcc taacgctacc cctgctaaag agacatgaga aagcgtatca
    24361 gccatcagcg attgtcttct gatcactaaa aacacaccta gcatcggggc gattccggca
    24421 atgaagatag cggcaagaaa cgctcttctc atgaattcat atgaaagcaa cgccattgag
    24481 aatcctcctt tctcaccagt cggatctgtc tgtccgcata ctgtttgata tcttcatgat
    24541 catgggtgat cattagaact gatttcccat ggacatgcgc actatgtcgg agaagacgat
    24601 aaaattcatt tctggattct tcatccatac ctgttgttgg ttcatcaaga acaaataagt
    24661 cagggtcagt agcaaaaata cgcgctaaac tgattcgctg cttttgtccg ccagacaatt
    24721 cacctattcg ccgatttcgc atatcccaca tccctacagc ttctaaagca cgctgtacat
    24781 gttgatgatc tttttcagag agacgcttga accagcggcc tcttggatat cttcctgaac
    24841 ggactaattc gatcaccgta ctaggaaaac ctgcattgaa agatgcaact tgttgaggaa
    24901 tataaccgat acttaatttt tttccttctt gatttttctc agcaatcttt atcattcctt
    24961 ttgaaggttt cagtaatcct aacgatgctt tcaccaaggt tgacttggct gctccatttt
    25021 ctccagttaa aatgacaaat tcacccgcat ctacatgata agagacatct tctagtacag
    25081 gttcatcatc atagtaaaag gtcaaatcag atatttcgat ataacgcacc ttacaacctc
    25141 cattctaaaa cttaattgat actttctttt aaagctttca gatttttttc catgacagaa
    25201 atataatttt cgcctgcttt gatttgttca ttagtcaagc tttctaaagg attcaacact
    25261 tctagcttca ctcctgtttc attcgacaat gtctcagcta ccttcgaaga ggcattttct
    25321 tcaaaataaa tcactttgat gttgtttttc ttcacaaatt cttttagctc agctaagcga
    25381 cttggtgtgg gttcctgatc tggtgaaacg cccgaaatcg ctacttgttt taagtcgtat
    25441 tgattcgcta aataagcaaa agcagcatgc tgagtgacaa atgtgcggtt tgttgcatct
    25501 tttagctcat cggagtattt ttgatccagt gcttcaagct ttttgatata agcatcggca
    25561 ttcttcgtaa aagtttcctt tttatctgga tatttttcgc ctaattcttc agcaatcgtt
    25621 ctgacttctt ttatagctaa taccggatcg agccaaacat gtggatcgta ttcatgtgaa
    25681 tgaccatgcc cttcttctga ttcttcatgt tcgtctgctt cttgcgattc taaacggtcg
    25741 attcctttag ctgcttcgat catcaaggtc tgtttgctat ccactgaatc ctttaacgaa
    25801 tccacaaaaa attccatgtc tgaactatta tacacgaaaa catctgcatc ggaaatttct
    25861 gctctttctt ttgcacttgg ttcgaaatca tgcggctccg ttccagcagg gatcaaaagt
    25921 ttcacgtttc cttcatcccc tacgatctct tttgtaaaat catacatcgg ataaaaagtc
    25981 gtaacgatcg tcaaatcctc ttttttgtct gcttccttgt tcgtatttcc acatgctgca
    26041 aacagtaaag cacctatcat taccgtagct gtcaaaagat attttttcat ttatatgctc
    26101 ctttcaatga acaaatcgga attttacgat ttattaacgt gaactaatgt atcaaaataa
    26161 aaaaacacag tcaatagaaa tccatcaatt tctgatttaa gcatgtatat tcttgattaa
    26221 atcgtaaaat ggatgagttg ctgctcatat ctagccattt cactacaata aaaaactgaa
    26281 gaataccatt ttaggattcc tcagtttttc atcacctatt ttacttctta tattttattg
    26341 cagggttcgt actaaataaa cttcctcaca aaaacctttt aacccattat atactctctg
    26401 cgctcttgaa taatgttgac acaaggcaaa aacggttggt ccgcttccac tcatcaatgc
    26461 agcatctgct ccgtacttga tcatacgctc tttgatttga tggatgactg gatgccgctt
    26521 tcccgttata tcttctaaac tgtttcccat ttttttgatc atctgctgat aatcttgctc
    26581 tcggatggct tcagcaagtc cgtggatgtc ttgatgttgg atggcttgcg tatctacttc
    26641 tctaaacact gtacgtgtag agacactcat tcttggtttg accaacacaa cccagcactg
    26701 tggcatagaa ggtaatactt ggatttcttc ccctttgcca ccgacaaatg ccgtacttcc
    26761 gtagacacag taaggaacat ctgtaccgac ttccaaaccg atttctgcca gttcttctaa
    26821 cgaacagccc agattccata attggttgat ccctcttaga gcagctgcac agtctgtact
    26881 tcctccgcct aatccagctg ctactggaat attcttctta acaaagatct tgacaccggt
    26941 tttgatattg tatttatttt ttagcaataa agcagcttga taaacattgt tccgttcatc
    27001 taccggtaaa aatgctttat ttgtttctac aatgattttg tcttctgtta tttcttcaaa
    27061 aaatagatga tctgccaagt ctacgctcga catcaccatt tccaactcat gatacccatc
    27121 ttgccgtcta tataatacat ccagtcctaa atttatcttg gctggcgctt tttcaatcaa
    27181 ttccatcctc tcacccattc gaaaagtcta agtcgcaagt attctatcat agggaaaaat
    27241 aaaagtatag cttatgtctg cctagtggtt gattttgtca tcgtttcaac agctttcaca
    27301 gaattttctc ttcataatcc aaacaagttc tagtgattct tataattctg tgagcacttc
    27361 ttctgccaaa ctcggaacat ttcctttttc ttttcctatc agactgagta gctgcatggt
    27421 agcaaatccg aaattacttc ctttcctata agcaaaataa cgtccttccc aatgatcgac
    27481 atattttaat ttcttttcat aagcagactg gaaggaatag aaaaaattac cataattata
    27541 tacgatatgc atgacttttt cttgaaagaa agaaaaatct gtttctccaa cattagctaa
    27601 tggagctaag cctagattgg cttccgagta gcccattttc tcgtatacaa taaacagatt
    27661 ggctaacaga tagtcagcta attcctcaga ttctttaggt aagacccgca acaaatcata
    27721 tcccgcctgc ttcttcgtca tcggctgagc ggtgatgaag ccaacgactt tttgatcttt
    27781 acggacaata ccgacaccgc tagataatac atatgattta tcaaatcgtc ccacagagaa
    27841 gtatttttct cgtgcacctt gaagccattt ttgtgaaact tcatttacag cttgatgtaa
    27901 ttcttccggc agttgatcgt accaagtaaa atggtatcct tctttcatca aacgttgata
    27961 ttcaagttgc tgactcagcc gagacaagtc ttctgttgct agatcaacaa tccctgcttc
    28021 acctactttc gtgaagtgaa aacccaattc atgaagcatc actacgtagc tttctgatat
    28081 cttataaaac gcaagctgat agcctaaaca atctgcttgt ttcataaact gattcgttgc
    28141 tgcccgcatc attttaggat tgccaactgg atctcctaaa acaaagcatt tgtttccttt
    28201 gatctgataa ccaaaaacga cttgatcttg gtcgtcttct tggtaataaa aataactgta
    28261 acctgacaag cgaagctgat ggcttgtagc tgtgcctccg tatgtttcca ataaacggtc
    28321 aaaacggtct ttttcgtact cactgccaag cttcgtagta tcttcagcta aatattgata
    28381 aagcgccact aaagctagca atgagatgcc caaaccaata agccctgaga accacacatc
    28441 atctgaagga aacaaaataa aatgatttcc tgttattcga ccgactaaaa agcgtccgct
    28501 gtgatagcct gcaattgcgt aaacgataaa caaaaagccg aacaagctag catcaaagat
    28561 tatagctccc caagaataaa cgaattttgt tcgataaaat tcttttctgg ataaccacac
    28621 agctaacaga atcaagacat ataccaatat caactgccaa gataacgtac gtgtgattgt
    28681 atttaaaata ccaaaagata gtaaagcaat cgtaggcaga aatgcctttt tgacttttac
    28741 tgaaagtgct ctagcaaggc cgagtaataa aaagccaacc aatagattca ctgtctgatc
    28801 taaaaaattg aatgagaacg gcaataatac ttgaaatagc cgactgattg cagacagatt
    28861 agtgattgtt gatagaagta ccatcatgat ccccgcaaaa tagacagcac ttacgagcac
    28921 gatatgcgca accttttgga aaaacagtcg cggaatgtta tctaaaaatc gattgatttt
    28981 gactcctgta tgtgtgatga acaatagaag tcccgtcata aaaggtagaa cataataaaa
    29041 caaccgataa tataatagcc aaactactgc tacatcttgc cgaacagaca attgactcat
    29101 tcctaagatc atcaaaacgt caaaagtccc cattccacct gggaccatcg tcagcattcc
    29161 tatcaatgta gcaatcatga acataggata gatcgaaagc atcgaaatat ctattctcat
    29221 caacttgccg atgatcaaaa ataccgcaag ggctcccgtc cattgtccaa aggatgccag
    29281 tagcagacca agcaagcctt ttggataaaa ctcgaagaac aatgtctttc tctttagata
    29341 agcaaaaaag aaaatgactg gtgctaaaaa actaccgccg aaaagccaga tccaatagct
    29401 tcgaaaaata ctttctgact gaataaaaaa gacatcaata aatgtaagaa acgaccatac
    29461 agacaaacca gtcagcataa atagtgcgac ttttgataca gtggctaaaa ccatttttct
    29521 atttgtctgc tttccataaa aactagcccg cagacttgct cctaccacac caccaaaacc
    29581 ggcgagattg ttgatcgtgt ttgttaccca cgcagcaaga agaaactctt ttctttccat
    29641 ttttggtttt ccttgttttt ctaagactct gatcgtcacc caatcataca acaacatcgg
    29701 agtgacaccc agcagtccag ccagaatcat gagcaggatc gtcatgcggc tttgttgtcc
    29761 catcctttgc caaatatctt gccaactcat gccattagca atatttgcga cttgatttgc
    29821 cacaaagact aacacggagc cgaaaaaaat cagttttaaa atcagactat gttcctttat
    29881 ccattgcagc atcaagtatc gtttattttt cacattttcc cattcctaac ttgtatactt
    29941 ttgtgtcata ccccaatgtt atcttattta ttttaatttt tccactttag acctgttctt
    30001 tgcttgcgat gatagaaaaa gaaagatatc ctaatgctaa aggagaggat aacatgaaag
    30061 taggatttat cggcacaggg aatatggcac aagctattat tgatggttta ttaaaaaaag
    30121 gaacagtctc gaaagaagag gtgttcgttt ccagtggaca ttttgaaaat gcacaaaaat
    30181 ttgctgctga aactggcgtc catgcatgca aaagtaatca ggaagttgca tcacaatcag
    30241 atatcgttct tttagccgtg aaaccaaaaa tcattgaaaa agtattggaa gaactaagag
    30301 aatatagcca gaaagtctta ttcatttcta ttgcttcagg aaaatcatta atagatttag
    30361 aaacagcgct tgaagatcct caggcctcca tcgtacgtgt gatgccgaat gtcaatgtca
    30421 gcgtaggagc aggcgtttct gcgatttgca aaaatgaagc tgccacggag gaacagcttc
    30481 agcaagctac gatgattttt gaagcaatcg gcagcgtcta tcctttagca gaatcagatt
    30541 tcagtacatt cattggcctt gctggcagct cacccgcatt cgtttacatg ctgatcgatg
    30601 cgatgggacg gactggtgta ctgaatggtt tgtccaaaca gacagccact gagattgctg
    30661 caaaagcaat tatgggttct tgtcaaaaat tcctttcaag tgataaaaat ccatgggaac
    30721 ttgtagacca agtttcttct cctggcggta caactgttgc aggtgtagtt gccttagaag
    30781 aagcaggatt catcccagca atcatcaaag gaatcaatac gacgatcgag aaagatcttg
    30841 agatgttgaa taaaaaataa tgatagcaat gttttttcgt gccttacaaa aaaagaagag
    30901 ctgtggcttt tgccgcggct cttcttttgt attttttatt atttagcata atcagttgca
    30961 cgagtttccc gaataacgat tactttgatg tgtccaggat aatccagttc ttcttcgatt
    31021 cgtttacgaa tatcacgtac taaacgaaca gcgtctaaat ccgagatttc ttctggcttg
    31081 accattaccc gaacttcgcg tccagcttga actgcgtaac ttgattctac accggcgaag
    31141 ctgttcgaaa tattttccag acttcttaaa cgacggatgt agttttctaa tgattcactt
    31201 cgcgcaccag gtcttgctgc tgagagcgca tctgctgcag ctacaaggac agagataaca
    31261 gaagtagctt ccacatctcc atgatgagaa gcgatcgcgt tgattactac tgggttttct
    31321 ttgtacttgg cagctaattc cgcaccgatc tcaacgtgag atccttcaat ctcatggtca
    31381 agtgctttcc caatatcatg gagcaacccg gcacgttttg ctaattggat atcttcgcca
    31441 agctctgctg ctaagatacc agctaatttt gcaacctcga ccgaatgctt caagacgttt
    31501 tgtccataac tagttctgaa tcgcagacgt ccgatgatct tgattagatc tggatgaagt
    31561 gtatgagcac cgacttcaaa cgctgcctct tccccgtatt cgcgaatacg ttcatccatt
    31621 tctttacgag atttctcgac catttcttcg atacgcgctg gatggattcg tccatcttgt
    31681 atcagttttt ccaaggtcat ccgtgcgatt tctcttcgga ttggatcgaa tccggaaaga
    31741 acaactgctt ccggtgtatc atcgatgatt aaatcgatac cagtcagcgt ctcaagcgtt
    31801 cgaatattac gaccttctcg tccaatgata cgacctttca tttcatcatt cggaagggta
    31861 acgactgaaa cagtcgtttc tgaaacttga tctgctgcgc agcgttgaat cgctaatgat
    31921 aataagtttt tcgctttgcg atctgcttct tctttggcac gctgttcaga ttcttttacc
    31981 attagagtta actcatgatt cagctcttct tcagtcgatt tcatgatgac atctcgtgcg
    32041 tcctcttttg ataaaccagc aattttttct agttcttttt gttgatcttc aatcattttt
    32101 tcaacgtctt tttctcgctc ttcaattaat tgctgttttg aactaagtgc ttcttcttta
    32161 ctttcaagtg agttctcgcg tttttctaac gagtcatctt tacgattcag cgtttgttcg
    32221 cgttgaatca aacgattctc ttgagatttt aattctaact tactttctct cagctcactt
    32281 tcaacttcag aacggtattt ctgattttct tccttagctt ctaacaatgc ttcttttttt
    32341 agagtttctg cttctttttt tgcactatcg atgattccag cagctgatga ttgtgcacca
    32401 gctatttctt tctcgtgacg tgactttgcg accataaacc ctaaaccaag accgacaatt
    32461 aaaccgatga tagcgaggag aatgttgaat accataaaat ccacctccat actatctttt
    32521 tcatttttat catctataca gcttctatga taaattattc taaactcgtc aatatgcatc
    32581 ttcgcatata tgtgtacaaa aaacatgcac aactttattc taatgtttgt accccgtagt
    32641 gtcaacttta aataaacaaa gaaacggctt tctacttatt cattaaacga tggtttttaa
    32701 aataaatgac actagtttca tataggtaaa cctttaaatt gagaaacttt tctattaaat
    32761 tattctttgt agatgaacag atatttttat tttatgtacc cactcgtata attaaataac
    32821 tcacgatttc tttattcaat aaaaaaacgg caggtcaagg agagtaccta ctcttcagac
    32881 cagccgtttt tgtttatcat aatttttatt cttctactaa aggcaattct tcttgtcctt
    32941 tttcatcttc tgggacagcg acttcttcac caataccata agctgctcta actagagcag
    33001 aaacttctgc catcatttca ggatgattag ccatatagat tttagcgttt tcccgtcctt
    33061 gaccaatccg atcttcttta taagaatacc aagcaccgct tttatcaaca atgtcttttt
    33121 ctactgccat gtctagaagt tctccttctt gtgagatccc taaaccatac atgacatcta
    33181 cttcagcgat tttgaaagga ggtgctactt tgttttttac gactttgatc tttgtacggt
    33241 taccaacaat atctgtacct tgtttcaatt gttctgcacg acgtacttcc aaacggatcg
    33301 ttgcgtagaa tttcaatgcg cgtcctccag gagtgatttc tggattcgca aacatcacgc
    33361 ctactttttc acggatttgg ttgatgaaaa tagcgattgt ttttgttttg ttgatcgaac
    33421 cagagagttt acgcaatgct tgagacatca aacgtgcttg taacccgaca tgtgagtcac
    33481 ccatttcgcc gtcgatttct gctcgtggaa ctaaagcagc aactgaatcg actactacga
    33541 tatctacagc cccacttgat actaaagcat cagcgatctc tagaccttgt tctcctgtgt
    33601 caggctgtga aagaagtagt tcatcgatat tcacacctaa tttttgtgca tattgcggat
    33661 ctaacgcatg ctcagcatca atgaaagcgg ccgttccgcc atttttttgt acttctgcaa
    33721 tagcgtgtag tgcaactgtt gttttacctg aactttcagg tccatatact tcgatgatac
    33781 gtccacgggg gtaaccgccg acacctaaag cgacatctaa cgcaagagag ccacttggaa
    33841 ttgtagagat ttgttggtcg attttttcgc ccaatttcat aatagagcct ttaccatagc
    33901 tcttttcaat ttttttcagt gcagcatcta aggctgcttt acgatcatct gccaatacga
    33961 taatcctcct tatatttaca aatcagagca acatttcatt atccttatca tatctttttt
    34021 agtgaaaaaa agcaagaaaa aaccgaacaa atattcgcat ttatcatttt cttaaaagct
    34081 gttttctcaa aagatcaaat ccttgcataa ccgcattata tctgatagca gcacgatctc
    34141 tggcaaagtg atatttttcc gaaaccacgc cgtctttaga tgaaatagcg atccatactg
    34201 ttcctgcaga ttgaccttct aacgtgtccg gtccagccac tccggtgaaa gctacactgt
    34261 aatctgttct tgctatctca cgtgcttgaa tggccatctg ctccacacat tctttgctga
    34321 ctgttccata ttctgctaat agctccggat caatctccag aaaaccagct tttgtttgga
    34381 gcgagtaggt aacgaatccg cctggaaata cttcagatac accagcaatg tcgcctaatg
    34441 cggcttggaa ggcgccggct gtcaaacttt ccgctgctgt tacagtctgc ttgttttctt
    34501 tcagtagttc cactacgact tttgctagcg aattatcatc gccgtatcca taaaaatatt
    34561 cccctactcg ttcttggatt ttttcttcta atgctaaaag agcttgattc cctgcctgaa
    34621 catcatttgt tttcactgtc agtcgtaatg tcacttcatt tggtttggca tatggtgcga
    34681 tcgttggatt gatctgtgtc tcaatcagat cttttagctc agtgaccagt cgcgattcac
    34741 caatcccata aaagcgcaaa actttagaaa ttagtttttc ttcagaagga aaacgttcct
    34801 ctagtaaagg acgtacttgt tccacaaaca ttggtttcaa ctcattaggc ggaccgggaa
    34861 gaaggatata cgcatgtgta tctgtttgat aaaaagtccc taaagcaagc cctgtacgat
    34921 ttggtagagg aaccccgcct tcgataatct gggactgttg cagattgttt ttggtcattt
    34981 ttcgatgggt tgtttcaaaa tatgccaata attttttgta tccttccgta ttttgtatga
    35041 gagacttacc gaggtgagcc gcagttgctt cttttgtcag gtcatcttct gtcggtccta
    35101 atccgccaca taagagtatg agttcacttc ttgtttcagc tagagcaatc aactcttcta
    35161 aacgctgctg attatctcct acaactgttt gataataaac ttcgatacct agatcagcta
    35221 gctgttctga caagaaagtg gcattcgtat tcacgacttg tcctagtaaa agttctgttc
    35281 cgacagctat tatttctgct ttcattactt cccgctccct tatatagtat acgctgtatt
    35341 tttatatttt tttatttaaa aagagggcaa ggagctgtga caaaaatctt gtcacagctc
    35401 cttgttccaa ataaacagtg ttctcctgcc aaaacctcgg tcataagtca caatatacat
    35461 aaaacatgaa aagctgtttt ttgaaattat cccttgtgac tcagagttag acggcaggct
    35521 cacaacctct ccactaaacg atacttacat tgatcctttg aacacaccgc tgtttttgat
    35581 gaagtaatcc acacctgaat agatagtaaa gatcagacaa gcataaagca tgatttgatc
    35641 tactggaata ccgatcaggc tgaatgggat attattgata aacagcaaga taatagcaac
    35701 catttgtgta gccgtcttga cttttcccgg ccaagctgca gccatgactt ctccatgttc
    35761 gacaagtagt aaacgcaagc ctgtgacggc taattcacga cagacaatga tcgctactac
    35821 ccaaccaggg gcctttcctt ggtcaaccaa catgataaaa gcagtcataa ctagcatttt
    35881 atctgccaac gggtccgcaa acttgccaaa attagtgaca aggcctcgcg cacgggcaat
    35941 ttttccatct aaccagtctg tgatactagc taaagcaaaa ataactgcac ctactagctg
    36001 tgtgacagcc agtgtggtgt ctcctacgtt aaattgtccc caatctaaag gtaaacctac
    36061 aaccaaaatg aaaatcggga tcatacaaat tctaagtaca gttaatttat ttggtaaatt
    36121 cacactatcg actcctttct tccctattct acgtgactat cagcgaagtt tccaccgata
    36181 ttatttactt ttccaaatga atttattcaa aggagtattt ttattgaaat tgatcttttt
    36241 gaattaacga agaaggaccg cgacacagat ttgtcacgat ccttcttttt tatacctaca
    36301 agtaaacaga tgcgtaaact aatttatacg atcattctgc gtattgaaga gtaaggttca
    36361 cattttttgg actctgattg ttttcccctg gattaactgg tacggattct ccattcgctt
    36421 tgatcgttgc attgcttgct gcaccaatcg tgatggtcgc atttgttgct gtttccggta
    36481 acacggtact ctgtgtttca tttgctgcca gtgtcccttg ataaacatag gcattgtcta
    36541 ctagcacacc taaccataca cgattgacag cagtaaattc aagggttaca ggcttggtag
    36601 cgtctgtgat atttactgcg atggcactgc cagaatcttc tcctgtagct attgccattt
    36661 tcttttcttc acttgatttt gttgttgatt ctactgtaga agactctttg gtctgcgtgc
    36721 tttgctctga ggaagaggac tcctcgactg atccttctac tttaagcgaa gaagctgtgt
    36781 ctccgattat tggatcagct tgacgatctt gccaagtcat atagcccacg acaacgacaa
    36841 tcgccaaagc aaccaatcct aataaaatga caggaagact tgtccaaaag cgacttggat
    36901 gcgattcttc ctgatgcatt gcttttctag aagtacttac tgtttcaggc tgcggacgtt
    36961 cagggaccga tcctgtcaat ttgcttttcc catcaaaaac atcgactaaa tggtctccat
    37021 cttctcctac agcttcagcg tattgccgaa taaatgcccg cacgtaaaat ttaccaggca
    37081 attggtcaaa atcatcattg tcgattgctt ccaaataacg tttttgaatt tttgtaatct
    37141 gctgtaattc atctaaagat atttttttat tcaggcgtgc ttgccgtaat ttttctccga
    37201 tggtttcaaa agccactcgt tcatcttctc cagtctgttt gattctccga agcatctgct
    37261 gttgcaacga aagacgttat gtttgggtgc tgtgacaaaa gtcatgtcac agctcctttt
    37321 ttccgaataa acattgttct cctgccaact cctcggtcac aagtcacaat atccaataaa
    37381 tataagaagc tgttttttga acttgttcct tgtgactcgg agttaaacgg caggctcacg
    37441 acctctttct taaacggtgt tcaatcagct gttcctcggt attagctcaa aaaccggcaa
    37501 aatataagga gatatttttc cgtatgtttc tctaatactc aggtcaaaac gctgatttca
    37561 caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagt ccaaccaagt
    37621 taaaaacatt aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    37681 tttcacaacc tcttggtaca cggtgttcca gaagtagctt cttcggcaat aagccgaaat
    37741 atcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac
    37801 ttctgtcaca acctctttga ttacttcgtt tgattttatt ttaatataag ttgattaaaa
    37861 gagagcatga tttgttttat tttctcacta atttttccaa cacaactaag aataccataa
    37921 aagctgattt tggaggaggt tcctactaca agtctaaaac gaaatttaag acttttcctc
    37981 ataaaatact ggtttatttt ggtctcatgt aaaactcgct gaaggcctct tcacttataa
    38041 atgcttcccc agctgccagt acgtctttca tttggatcga atcgatgatt tcaggaagat
    38101 caaataaagt tcgatcgcca aataaacttt gcgtaaattg attggcaata tattcgatcg
    38161 aatttaatga ttggaaatat tgtccaagca ttttcttctt cagcaaatcg agatttgttt
    38221 ctgacacttc cggatcatca gcgaatccaa gaatgatttc tttgactttt tctgctgctt
    38281 tctctggttc gtccgtatct ccgctgaagt ccgcaaagtg aaattcacga tccaagctga
    38341 attcaaaacc aaagctgtca tcgataattc cttgattgta catcgctaga tagtttcgag
    38401 aagtattgcc taaaatcatt tggaacaaca gattgattgc tgttttatag cgtaacagtt
    38461 ctgttccttc ttgcggcaat gtgtctaatc ctttaatacc aagtacaaat ttatctcgtg
    38521 taatggctgc ttccaatgca ctttgtttga tgatttcttt tgtgttttca gggaaataac
    38581 gtacaatttc ttgctttgga gggaaatttt ttgcttcttg gttttcccga ataagtttca
    38641 tcaatttctc aggctccatc ttaccaacta caaataaaac catattgctt ggctgataaa
    38701 acgtccgata gcacgtatac aagtcttgag ctgtgatttt gtcgatactt tcaaccgtgc
    38761 ccgcaatatc gatatgcaat gggtgtgtag gatacaagtt atttagtatg ccaaaaaaca
    38821 tccgccagtt agggtcgtct tcatacattt ggatttcctg accgatgatt cctttttcct
    38881 tgtttacagt ttcttctgtg aagtaaggtg cttgtacaaa atcgatcaga gtcgtcagat
    38941 tcttttctac ctgatccgtc gtagaaaaca gatagctggt tttcgtaaaa cttgtgaaag
    39001 cattggcgga agctccttgt ttcccaaata attgaaagac atcaccatct tctttttcaa
    39061 acaacttatg ttctaaaaaa tgggcaattc catctggaac tttcaccttt tctttttcac
    39121 catagggaat aaattcgtta tcgatcgaac cataattcgt actgaacaaa ccatatgtct
    39181 tgtgatagtc atttttaggc aacaaataca cggtcaaacc gtttggcagt acttcatgat
    39241 aaagtgtttc attgatttgc tcatattctg ttttattcat ttgcatttcc tcctgctaaa
    39301 aagaagattg cctgcaattc gatttgctca gctacttgtt gtatctcttt gatcgttacg
    39361 ctctccatac gtttcaacca ttcttcatca gataattttg tttccggcag ccagctgtcc
    39421 aaaaaagaag cttcgattgc cgcttgcgga gagtctagag aaagcagata ttggtttctc
    39481 agcattgctt ttgtctgaga cagttccaac tctgttattt caccatttcg caaactttct
    39541 aattgctcgt gaatcagatg aagtacttga tttcggttct tttcatcgat accggtttgg
    39601 acactcatga atcctcggaa cgtatccacg ctactagagg cataataagc tagactttct
    39661 ttctctctga cattcatgaa aagtttagag tgaggaaaac cgccaaataa accgttaaag
    39721 accattagcg caaaacgttc tggttcatcg taatacacat ttgtctgata agctaggttc
    39781 agctttgctt gaacgattgg ctcacgtact tgtcgttcct tcacgatatt cacttgaggt
    39841 tgggtataaa acagatctgg ataaactgtt tcccgcgact caaatggtaa ttgcccaact
    39901 gcttcagctg ctttgtccgg atcaacatcg ccgacaacaa aaatgtcgat ttgatcattt
    39961 gccatcattt cttgatagta ggacgctagc gaactagcag tgatcttgtc caaatcttcg
    40021 actacgccca accctgggat cttttgttcc gggctgtttt caaaataaag ttcttgcaag
    40081 gcataggaag caaaagtttg tttatcttcc ttcaaacttt ccaaatatag acgcagattg
    40141 ttcttttcca agtcaaacgt caactgatca aactgatgat ttttaatatt tggataaaat
    40201 aaaacctctt ttaaaaaatc taccgcttga gaaaaaaggt ttggatcatt gacatattca
    40261 ccattcacaa aactgatccc tgcgtttagc caatgtaaat ttcctttttt tctaactgat
    40321 aatccaaagc ttgcgccata aagctctgca agttgcgcac ttaactttgt ctgatccgaa
    40381 taattcaaac tatttgtttc caacatgctg ctgagaagtg aacgtttcgt gatgacttct
    40441 tgttgcagac gcgcagtaaa acggataaaa atccggatcg ttttgtattg ttttgttggt
    40501 aagacatgaa gattcacgcc ttctgccaat tgatacacca tatttatatg ctccttttca
    40561 cagttgattg cccgcttact cttttaatgg gtcaacctgc cttttttact tattcctctg
    40621 cttgaaattt ataagttcag gcaattcgcc gttttttcta aatgatgtta cactttatag
    40681 tatagcatag atgtttttta tcaaaagaaa cagacggcta aaagcgtata ttgttttttt
    40741 attttttatt tttcgatata ctaaagacaa ataaaagaag ggagggtcat tcatgatcaa
    40801 agaattcaaa gagttcatca tgcgtggaga tgtcttggat cttgctgtcg gcgttgttat
    40861 tggtagtgcg tttacaagca tcgtaaacca agtggtgcaa ggcttgatca caccactaat
    40921 aggttggttt gtcgcaatga tcacaggtac ttctgattta gaaggagcac tttcaattct
    40981 tgactggtcg ccgacaaaag gcgtcacatt tgcgtttggt aacgttatta gtgctatcat
    41041 tacatttctg atcactggtt ttgtcttatt cttagttgtc aaagcggcaa accgtgcgaa
    41101 gaatttacgc tccaaagaag agcaagtgga agtggaagaa actcctactg cagaagatta
    41161 tcttagtgac atacgcgatc ttcttgccaa acaagtggca gaagacaata cacatccagt
    41221 agagcaaaca aatctttccg atacttctaa ataataaaga atctaaaaaa aagaaagccg
    41281 attttcagtg tcggctttct ttttttagat tcttaacttg ctacttgttc ctttttcatg
    41341 tcgatatgtg gaatatcatc ttccaaatac acatctgaga ttgccttgaa tccaaacgac
    41401 tcataaaaag cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc tggaaaattt
    41461 ttctggatca tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc tttacggttg
    41521 tcctgatgaa caaggacacg tccaaaatgg atactctccg cttcttcata tactcgcgtg
    41581 taggcaagga tcttcccatc agtatcttga aaaaacgtat gccaagcttc tccatcaatg
    41641 tcatcaactt cttgataagg acactgttgt tccacaacaa aaactgcgat tcgttctttg
    41701 acgatcgcgt aaaactctgc tgttgacaat tcttcaaatt tctttacctg atattccact
    41761 aagcaaacgc cccctgcttt tttattctta gcataccgat tcgcacacaa aaaagcaaac
    41821 gaaccttacg aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct
    41881 caactgtccg agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt
    41941 caggatccaa atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt
    42001 ctgtttgacg ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt
    42061 ctaaatcttt cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact
    42121 ataccacata tttttaaaat tttcaagttt accataaaaa aaaaagaaat gcaataaaaa
    42181 taaataagaa gctcattaag atttttctaa attattcctc attataaaat ttcaagtgtt
    42241 tttttatttt gagttttact ctcccgtaag cgctcattac ttgcttgtga tccattgcta
    42301 aacattcagc catgtctaca aaatcaacgc cttgcatata ataatgaaaa attttagctt
    42361 ctatttttga aaactggatt tcgcaatttt cgattgcttc ttttaataaa agatgctgaa
    42421 ttgctaaagg ttcttgcaat gtgatctctg ctaaaaagtc tggtccttct gtctctattt
    42481 tttggtcgat tgagacttca tctttttgat cacgacgctt cagcgcgttc tgcttcctca
    42541 acagagaata gatttgattg cttataacgc ttttataata acttccaaag ctaacacctt
    42601 tgcttttttc gtaagttctc aatgcctgat ataaagcgat ccttgcttct tgttcccaat
    42661 cctcagaaac ataatttctt aaataatagg tgtttttcaa acgtagtaaa agtggaaaat
    42721 atttttgata cagggcagca aatgcttctt cgtcaccatt acgtgcagcg cgcaataact
    42781 cctcagacat aaaataattc ctccataaat tcgtttatgg aggaatctta tcatcaatga
    42841 ttcatcgaac tgattagaac gatatgttat ttattttttc tcattaaatc gtccaatttt
    42901 tgggacaatt tttttaactg ctcttcattc catggagaat tacgtcggaa atcaataaaa
    42961 tgcatatctt tattttcttg ggctattgtt ttttccgttt tttttatcgt cttatataat
    43021 tcataagcag aagttcgtaa tgctccttgg gaaaagatca tccactgttc tgctaaatcg
    43081 cttgtcgcca ctgtcacttg tgtcaaacgg tcattcagct gaccagcgat tctctcgata
    43141 taactgtcag ccgtttcgtc ttctttcgta aaaacaacat ccaactggta tttcttataa
    43201 ttttgttgga tgcccggaac tagttgggca tcgaagacaa cgatgacttc cattccttca
    43261 tatttcgcat aatttgataa acgatgtaaa agagcttctc ttgcatccgc cattttgtcc
    43321 tgctttttaa gctggacaag ttccggccac gaaccaatca tgttgtagcc gtctacaatc
    43381 aacagctgtt ttttcatggt ctactccccc tgacgttggg aaaaagcctg atacataagc
    43441 aaaccagcag caacactggc atttaagctc tggacgtgcc cggtcatcgg gatcgtcagc
    43501 aattcatcca tttctttttt caatcctgga ctcattcctt tgccttcatt accaatgact
    43561 aaagcaattg atcctttggc attccatttt tggaagtttg tgccggtcat atctgtgcca
    43621 aatacccaaa atcctgtttc tttgagctgt tgcacagctt gtaccaaatt cgtcacgcgt
    43681 gcaacaggga catgttcgac tgccccagtg gaagtctttg ttacgatagg tgtgattcca
    43741 actgctcggt gttttggtat gatcacacca tcgacgccac ttgcgtctgc tgtccgtaaa
    43801 atcgacccaa aattgtgtgg atcttcgatg ctatccaaaa tcaaaaagaa ggggtcttct
    43861 tttttctctt ttgttcgttc aatcagttgg tcaagggata aatattcata aggtgtaatc
    43921 gcaagaacga ctccttgatg cacgccgtga tcgctcaaag tttctaattt ttgtttagga
    43981 acccatttga ctggcacagc ttgttcacgt gcgatttctt tcagttcttc tactttttct
    44041 cctcgtgaat cttcttgaag aaagagttta ttccctcgcc cttgttgtag cgcttctact
    44101 gcggcatggt gcccaaagac gaaattatct tctaatgctt cttcattttg atcagaaact
    44161 gactgatttc gtccagtttg ctttttattt gaatatctat tttgtttttt gtcttcaaat
    44221 ccacgtttcg attgatgttt gtttgttctt tctttaccca tttttttcac caactttctg
    44281 aatacaccag cggatcaatt cttccatccg ctcggtttgt tttgtcaaat gcagatagcc
    44341 catgagcgct tcgaaccctg ttgcgacccg atacgtcgta acatcagcat tttttgcgct
    44401 cgtatggctt ttagcatttc ttcctctgcg ataataaagt tcttcttctt ctgtcaaaag
    44461 atgttcttcc agcatttgct gaatcaaaaa ggcttgtgca ttcgcagaaa cataattcgt
    44521 tgccgcacga tgcagcttat tgggttttgt ttctccttga tcaatcaggt ggtcacgaac
    44581 atagatttcg taaatcgcat ctccaacata agccaaggct aaaccgttca attgtgtgta
    44641 atctctcatg cttctcttct ccatcgggtt ccttgcggtg tatcttctaa taagatccct
    44701 ttttctttca acaaatcacg aatttcgtcg cttcggacaa aatcacgatt gcttcttgct
    44761 tgatttcgtt cttcgatcaa ttgttcaatc tcttcatcta ataactcttc tgctgtaaat
    44821 aaaataccga agatcgaaag ccattgcgag aataaagctt ctccttgtgc caagacagcg
    44881 gcagaaactt tcggctggtc tagataagtg ttcagccact tagcaagctc atagaccact
    44941 gtgatcccat ttgctgcatt aaaatcatcg tccatttctt cgatgaatcg ctgttctaaa
    45001 ttttctaaca aagcgatttt atccgcatca tctgccaaat tctctgaggc agtttcttgg
    45061 cggaatttca aattgtcaaa cgtattcttc agtcgttgta aattggtggc tgcttctttc
    45121 atcgtcgttt cactgtagcg gatcggacga cggtattgtg tggtcgccat gaagaaacgc
    45181 aagacttgtg gatcaacgtt tttgatcatt tcatgtacag tgatgaaatt ccctagagat
    45241 ttactcattt tttcatcgtc ttcgccaatc gttacatagc cattgtgcat ccagtaatta
    45301 gcgaattttt tccctgtttt cgcttcactt tgtgcgattt cattttcatg atgaggaaat
    45361 tctaaatctt gcccgccacc atggatatcg atcgtttctc ctaaatgttt tgttgccata
    45421 acggaacatt caatatgcca accgggacgt cctttgcccc atggtgaatc ccaagaaatc
    45481 tcgccaggtt ttgcttgttt ccataaagcg aaatctaatg ggtcttcttt ttgctgctgt
    45541 tcttcccctg tacgctgact tgctccgact tccagttcat cgatggattg gtcacttaat
    45601 ttcccataat tttcaaattt acgggtacgg taatacacat cacctgaaga ttcataagca
    45661 tagccttttt caaccaacac ttgaataaat gctaaaatat ctggcatatg atccatgact
    45721 cttggatgca gtgtagcagg ttcgacattc aacgctttcg tatcttcttc aaatgctcgg
    45781 atgaaacgat cagcaacttc tggtgctgta atacctaatt cgtttgctgt acggatgatt
    45841 ttatcgtcta catccgtgaa attcgatacg aaatcgactt tatagcctcg atattcaaaa
    45901 tagcgtcgaa ttgtatcgaa agcaatggtg ctacgagcat ttccgatatg gatataatta
    45961 tacacagtcg gtccgcagac atacataccg acatgtccct tttgtatagg gatgaattct
    46021 tctttttttc tggtcaatgt attgtagatt tttatcattc tgatctcctc actttttctc
    46081 ctttgattcg aacgactttt gctggtactc ctaccgctgt agcgtcgtct ggaacatctt
    46141 tcaacacaac ggcacctgca ccgattttgg cgttttttcc gatcgttacc ggtcctaata
    46201 tctgcgcatt cgcagagagc atggctcctt gtttaacagt tgggtgtctt ttacccgttt
    46261 cttttcctgt gccgcctaat gttacgccat ggaataaaat cacatcgtcc tcgatttctg
    46321 cggtttcgcc aatgacgatt cccattccat ggtcgatgaa cacgccagtt ccaatcgttg
    46381 ctcctggatg gatctcaaca ccagttagga aacgccagaa ttgagcattg atcttcgcca
    46441 ataaaaagag ccggtgctga tataaaaaat gagagaaacg atgccaaaac aaggcatgaa
    46501 aaccagggta tgtcaaaacg atctcagctg tcgaacgtgc agccggatcg tttctctttg
    46561 ctgcatcaat cgctcgggta aaccaactca tgactttcca ctcctttcgt aagaatttta
    46621 tgtaggaaat catcttctta taaagaaaag aagcgccttt taaataagtt tccttatcaa
    46681 aagacgcttt ccgcgtggtt ccacttttct tcataatcaa tgattatctc tgacagctaa
    46741 cgcctgttat acgttcttgg ctactttgtt tcctataaaa cagtttttcc tgttttctgt
    46801 gtttcgccaa gaccactccc agaggcattt cggcaaccat tcattgatac cttgcaccac
    46861 ccggtatctc tcttgtaatg atcagctgct tacttttttc tgttcatcgt gtttagttca
    46921 gtacttgatt taaatgtgct aatgattttt cacgaccaag caattcgatc gtttcaggca
    46981 attctggacc atgcatttgt cctgatacag ctacacggat tggcatgaaa agattttttc
    47041 cttttacgcc agtttctttt tgaacagctt tgatggcagc tttgacagtt ggagcatcta
    47101 ctacttccat ttgttctagc tgtgctttga atgcgtggag aacagtagga actgtttcac
    47161 cagcaagtac ttcttttgct gcttcattca atacaggatg ttcattgaaa aatagcgtag
    47221 acagatcaac gatttctgca gcatagctca tttgaggttg atacaggcta acgatcttct
    47281 tcacccattc gattgtcttt tcatctgggt tttctgctac acgtccatca gcgattagat
    47341 aaggcagaca catgtcagta aatgcatcta gatccattgc tttgatgtaa tggttgttta
    47401 cccattgcag ttttttcgca tcgaaagctg cgggtgattt acttaggcgt tttggatcga
    47461 acattttgat caattcttgg tgttcgaaga tttcgtcttc gcccacaggt gaccagccca
    47521 ataatgcaat aaagttgaac attgcatctg gtagatagcc taattcgcga tattgttcga
    47581 taaattgcaa gattgattcg tcacgtttgc ttagtttttt acctgtttca ctgttgatga
    47641 tcaatgtcat atgtccgaag gttggtggtg tccagccaaa tgcttcatag atcatcagtt
    47701 gttttggtgt attggcaata tggtcatctc cacgtaaaac gtgagtgatt ttcatcaaat
    47761 gatcatcgac cgcaacagca aaattataag ttggcatacc atcgcgtttt tggatgacaa
    47821 agtcgccacc gatattatcg gattcgaacg aaatagcgcc tttcaccata tctgtaaatg
    47881 cgtattctgt attgcgaggc acacggaaac gtacaacaga ttctagacct tgtgcttctt
    47941 tttctgcttg ttcttcaggt gtcaagttcg cacatgttcc tgcgtaatga ggcatttctc
    48001 ctcttgcgcg ttgtgcttcc cgttctgctt ctaattcttc ttctgtgcaa tagcatttgt
    48061 atgctcgatt gctagcaagc agctgatcaa tcagcggttg atagatttct ttacgttcag
    48121 attgacggta agggccgtat tctccaggat tttcaggaga ttcatcccaa ttcattccca
    48181 accaagcaag gttttccaac tggctttttt cgccatcttc gatattacgt ttttggtcag
    48241 tatcctcaat acgaataata aagtctccat cattgtggcg ggcaaacaaa taattgaata
    48301 atgcagttct tgcatttcca atatgcaggt gcccagttgg acttggtgca tagcgtacgc
    48361 gtactttcgt cattttcttc ctcttctttc aaactagact ttgactaaca gcaaaaattc
    48421 cgtcagttac ggcttctcaa atatcattta tttaaagcca ctcagcacaa attttacctt
    48481 ttctaaggta aatagtaaag ccaactggtt gaatttcctt accagttggc tgaatttctt
    48541 ttttattttg attgtttagt ctttttctct gtctcttttt tattgtcgat tcctcgtcct
    48601 gcatgtgcag gtttagcaaa gatcatgcgt ccagcagctg tttgaagggc acttgttaca
    48661 acgacttcga tacgttcatt catgtaatgc tgtccatctt ctacgacaac catcgtgccg
    48721 tcatcaaggt aggctactcc ttgttggcgt tctgtcccgg cttttactac tagaacattc
    48781 atcgtttcac cagggatgac gactggtttt acggcatttg ccaacgcatt gatattaaga
    48841 acaggtacat tttggaattc agagacttta ttcaagttat agtcattggt tacaatgaca
    48901 ccatctaaaa gcttagccaa tttgatcaat ttgctgtcta cttcactgat atcttcaaag
    48961 tctccatcgt acatttcgac agatataccc tcttcttttt gcaaggcatt taggatatct
    49021 aatccgcgac gaccacgcac acgtttcaaa ctatcccctg agtccgcgat gtattgtagt
    49081 tcgtaaagaa caaaattagg gatcatcaag gttccttcta agaatccagt ctttgtaata
    49141 tcataaatcc gaccgtcaat gatcacgctt gtatccaaga ttttatattt atggaaattt
    49201 tctcctgatt ttcgttctaa cagttgactc tcaacttgac tagctgtttc ttgctcgatt
    49261 tttttgcttc gtgaaccaaa aactttcttc cattcttcga ttcgtgtcgt tcccattctc
    49321 aatcccagat agcctaagat gatcatcagt aaaatcggca atacactatt gacgaatgga
    49381 atttccatat tgtaaagagg ggtcgagatc acaacaccta aagtcaatcc gatgatcgca
    49441 ccgatcgcac cgaagagaag ataagtcaag ctgacctcac ttagtttttg ttctacttgt
    49501 ttgataatag ctgagatatg tttggccaac agcaaagata ataaaaagaa aataagtgcg
    49561 cctaacaggc tgttggtaaa tgtattattc agccatccgt ttgactgttg accgattgcc
    49621 tgccaagctg agggtaaaaa tgaaataccc aagcttgcgc cgacaatgat catcagcaaa
    49681 gtaatgacac gtttttgcat cgtttttcct ccgtttcgac aatcatttat tagttagcgg
    49741 aacacacgct tcaacgtttc agccaaagtt gctactccaa ctatctcgat tccttttggc
    49801 gcttcccagt tacctaagtt gtttttaggt aggtatactt ttgtgaagcc gagtttttgg
    49861 acttctttga ctcgttgttc aatatgactg acacgtctga tttctccagt cagtccaatt
    49921 tcaccgatga aacattccgt tggctgtgtt cctttttctt tatagcttga agcaatactg
    49981 acagcgatag ctaaatcaat cgctggttca tttaatttta ccccacctgc agcttttaag
    50041 taagcatctt gattctgaag aagcaaccca gctctttttt ccagcactgc catgatcaga
    50101 gatacccgat tgaaatcaag acctgttgtt gttcgtttgg catttccgaa catagtaggt
    50161 gtgactaatg cttggatctc taccaaaatt ggacgtgtcc cttccattgc cacaacgata
    50221 gcagagccag ttgcaccgtc taggcgttct tctaagaaaa tctgtgaagg attagcgact
    50281 tcttccaatc catgctcatg catttcaaaa atcccaatct cattcgttga gccaaatcgg
    50341 ttttttactg ctcgcaaaat ccgaaaacta tgatgttgtt caccttcaaa atataacacc
    50401 gtatccacca tatgttctaa cattcttggg ccggcgatcg agccttcttt ggtaacatga
    50461 ccgacgataa aaatggctat cccatttgtt ttagcggttt tgagtagttc tgcagtggtt
    50521 tctcttactt ggctgacact tcctgccaca cttgtgatgt ctggctgcgt catcgtttgg
    50581 atggaatcaa tgataacata atcaggggag atatgttcga tggcacgact gatttcgttc
    50641 atatctgttt cagcgtatag ataaaattct gtgttgatac ttccgaggcg ttccgcgcgt
    50701 aattttatct gctctgcgct ttcttcgccg gatacatata agacttttcc gcctatggct
    50761 gctaattgct gagaaacttg taacagcaaa gtggatttcc cgatacctgg atcgccacca
    50821 atcaagacca tagaccctgg caccacacct ccgcctaaca cacggttcag ttcttctaat
    50881 tttgttttga tccgaggttc ttttttaggg acgacgtctg cgatttttgt tggttttgct
    50941 ttttctcctg tcaaacttgt ccgagttctg cggtccgttg tatcttgttc gatttcttcg
    51001 accatcgtat tccattttcc gcaatttggg cagcgtccca agaacttagg cgacacatac
    51061 ccgcaatttt ggcaaacaaa ttgtactttt gatttttttg ccattgtacc gcctcctttt
    51121 atcctaatca tgagacatta aatcaagctt aagtatcttt aacgttttat ttactggaag
    51181 aaccaaatcc gccttgacgt tgagcagtcg tttgatcttg atctgctttt aaaaacggca
    51241 aaaagatccc ttggccgata cgctcgcctt ttttgatcac tacatccttg aaaccaaagt
    51301 tggtgaattg gaacatgata tggccttcat ttgcctcatt attgtaataa tcactgtcaa
    51361 tcacacctac tccgtttgtc aacactaaaa accgcttcaa cggattgctt gaacggttag
    51421 caagctgcaa aaattcatct tctcccatgt aagctttgat tccagtaggc acgagggttg
    51481 gtttcaattg tttttcattt tctgatacat cttttttcaa tccatctgcg actgtttcca
    51541 accccttttt ccagcctgct ttccagatac ttggaatcac gacctcttct gctgcttcaa
    51601 aatcatagcc agcagcatga tgtgtagcgc gctgcggtag attgatattt tgatctttat
    51661 atttagaaat aacttcaaat cctctttttc tcaccggttt tcctccctaa ataaactact
    51721 cctattctaa catatccgag gctgtaaaag actttcttta caaattcttc aagaaattct
    51781 gctatcttaa agtaaaccat taattcgttc tttttccaaa cgactgcaag ttgtacaaaa
    51841 aaactatttt ttacatgact ttactcgtca aaacaagtag aatagaacga aattaatgag
    51901 tggatatttg attgaggtgt cacatgcgta aagaaattaa aaatgcaaaa cgaattattg
    51961 tcaaagtagg aacaagtaca ttgatttatc ccaatggaaa tatcaatctt gcggcaatcg
    52021 atcagttagc ttttgtcctt tctgatctta gaaatcgggg aaaggaagtg atcttagtct
    52081 catcaggagc tgtaggcgtt ggtatgcata aattacattt gtcagaacgt ccgactttga
    52141 ttcctgaaca gcaagctgcg gcggctgttg gtcaagccga actgatgaat atctataatc
    52201 aaagattcag tgtatataat caacagacgg cacagatcct cgtgacaaga gacgtggtag
    52261 attatcctga aagtcgtaaa aatgtgagga atacattgga acagcttttg attatggaca
    52321 cgatccctat cgtgaacgaa aacgatactg tagcagtgga tgaattagat catctgacaa
    52381 aatttggcga taatgatcag ctgtcagcca tcatggcaaa acttgtcgcg gcagaccttt
    52441 tgatcgtcct ttctgatatc gacgggtttt attcagataa tccgactaca aatcccgagg
    52501 ctcagatgta tcgctatatc gataaaatcg atgaaaagat tctggcacaa gcaggtggtg
    52561 ctggtagcac atttggaaca ggcggtatgc agagtaaatt aaaagccgct tctaggatat
    52621 ttgaagcaaa cagcgcaatg attttagcaa atggaaaaaa tccggcaatt atttttgata
    52681 ttcttgcagg aaaagaagta ggtactttat ttaaggagga cttgaaatga ctgacttgat
    52741 gcaattagga caacaggcaa aagatagcgc gcaacaacta gcacttatgc ctacaaaccg
    52801 caaaaatgag ctacttgttc aaatggcaca aaccatcaaa aagaatcaac aagcgatcat
    52861 tgaagcaaat cataaagact tagaaaaagc agtcgaaaac aatatcagtg aaacgatgca
    52921 agaccgcttg cagctgacac ctgagcgaat caatgccatg gctacagaga tcgaaaaaat
    52981 tgcttcgtta gacgatccta ttggaaaagt agatgagatg tggacgaaca ccgacggatt
    53041 gagaatcggc aaaaagcgcg tgccactagg cgtgatcggc atcatttacg aatctcgtcc
    53101 aaatgtcacg acagatgcag ctagtctagc attcaaatca gggaatgccg ttatccttcg
    53161 cggtggcaaa gaagcattct tctcgaatca attattagtc caattattgc agcaagtact
    53221 ggtcagtgct ggcgaatctc cttatgccat ccagtttgtc gatgatactt ctcatgaaac
    53281 tgcaggacag ttgatgcgat tgacagaata cttagatgtg ctgattcctc gaggcggagc
    53341 aaaattaatc caacgagtca aagaacaggc aacggtacct atcattgaaa caggtacagg
    53401 aaataatcac gtatatatcg ataaagaagc acagttatct atggcagtga acatcatcgt
    53461 caatgcaaaa gcttcccgtc catctgtctg taacgcggca gaaacattgc tgatccattc
    53521 tgagatcgca ccttttttct tgccagctat tgaaaaagaa ctggtagaac acggggtctc
    53581 tttacgtgcg gatgctcgtg cgttagaata tctagagacg gctgctttag cagaagacac
    53641 agattgggat actgaatatc tagattatat ccttgctgtc aaagtggtcg attctttgga
    53701 tgaagcaatt gcacatatca atcggcataa tactaaacat tcagaaacga tcgtgacaga
    53761 tagttatgcg gctagtcaac gtttcttaaa tgaagtggac gcggcggctg tctatgtcaa
    53821 tgcctccact cgttttacag acggatcggt atttggtttt ggtgcagaga ttggtatctc
    53881 tactcaaaag ttgcatgccc gtggtccaat ggggttaaat gaattaactt ccacaaaata
    53941 tatcatttat ggcgatggac aaatccgcga ataatgtttc gtgtgaaaca ttattcccgc
    54001 cttttgcctt ttttctgtaa aggttctatc caactagaaa ggtgtcaata tgtttgaaat
    54061 tttaatgatt ttttttattt accttatttc tctgaatatc gctgcttttc tgggagtcgg
    54121 tattttatcc cttttctttc aattcaaaaa acgaagcata gggagtcaac gggaaaaatg
    54181 gtcgcagtat tttgacaaaa tcgggcaaaa agggctggtt acacgcttgc acattagtta
    54241 catggttgca ttatgcttat tagcgacaat caattactat tcttttttcg atcattcaat
    54301 cgcttatacg attacgctac tgattgctgg gatttttcac ttatcttata aatatcagct
    54361 gaataaaaac cacttgaatc ggactttccg ctaaaaaata tccgtacgat aagtagagtg
    54421 ccttgaagat cggcaaaaaa ctgccggact ttttagaggc tgtgacaaag attttgtcac
    54481 agcctctttt tccatataaa cggcgttcgc ctaccagctg cctggtaaat ctgtttcctc
    54541 ttcgttaaga agcgaaaatc cgtcaaatgt ttcgccatca taataacgaa gcagctcatc
    54601 catggacaag ttcatcgttt tttctttttc ttcttccgct gattcagcca gtagctgttc
    54661 ccgatacacc tgatacatac cagctatttt ttgctgcatc gcttttctag tttcaccttc
    54721 cgcattgatt attggcaatt ctttgatata gccacgataa atcaaccgat catctgaaaa
    54781 ttcaatgggt aattcaacga gtgtcaaccc gacttcctga aaatattctt ccagcatcta
    54841 ttatcccccc attctttttc ttaaatgaat tttagcttac ctactgaaaa ttttccagtt
    54901 tcattatttt gagaaaaaaa gggaatgtga caaaaatcgt gtcacatccc ctttttccga
    54961 ataaatggtg ttcttctgcc aactcctcaa tcataagtca caatattcaa caaacatgag
    55021 aaactgtttt ttgaaattgt tccttgtgat tcagagttaa acgacagtct cacaacctct
    55081 tggtaaacgg cgttcaatca gctgaccctc ggtattagct caaaaaacgg taaaatatga
    55141 gaagctattt ttccgttttg tttctctaat acttaggata aaacgctgat tttacaacct
    55201 ctgttaaacg gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg gcaaaatatg
    55261 aggaactatt tttccgtttg tttctctaat actcaggaca aaacgctgat tttacaacct
    55321 cctgataaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
    55381 atgagaagct atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa
    55441 cctctgattt cttattcttc ttcatttgta gcaaaataat tacggtgata aatgatacaa
    55501 catacactga taagaacaat ggcacctgca aaataaaacg catagctttc accaatatgt
    55561 tcaccaatca cacctgcaat aaatggtgct aaagctccac cgaaaaagcg gacaaaacta
    55621 tagctggacg aagcaacgtt tcgttgcaga cctggtattt ccatagcaat cgtcgtcaac
    55681 agtgtattga taattccttg gaaaaatcct gctaacacaa tgctgatcgg tacaaggact
    55741 ggcatagaag ttccagctcc taataacagc aaacaaataa tgaataagac aaatgctgct
    55801 aaaattgtcc gataagtcgt aaacttccgc tcaagagccg gtgcaacgaa aatcgaagca
    55861 atagccagaa gcacacccca gccaaaaaat acaaaaccga cttctaattc agaatagcca
    55921 atcaataaaa atggcgcata cgcaagtaaa gtaaagaatc cgaaattata taacaatgcg
    55981 attagtccaa ctgaacgaag tttatgattg cttaacgctt tgacaccttc ccaaaggcct
    56041 acttttctct ttggtttctc agtttcaggt aaaagtaccg ctagggctat aaaaccgcaa
    56101 aacatcaaaa atgctacccc aaaaaatgga taacgccatg aaccactgcc caagacccca
    56161 ccgactagag ggccaacagc cattccgcag ccaagtgctg cttcatacat catgattgct
    56221 ttttctgtgt taccaactaa aatactgacg atcgttgcta aagcagtaga gacaaataaa
    56281 gcattcccga caccccagcc ggcacgtaac ccaatcagca ttccgatatt cccagataat
    56341 ccgccaagac cagcgaatac aacgatgata aacaatcctg tcatcaatgt ttttttcaaa
    56401 ccgatccgac tagataagaa acctgtaaat agcatcacca caccagtgac taacatgtaa
    56461 ctagtaaata acagggtcgt ttcagctggc gtagcgttta actgtgcagc aatcgttttt
    56521 aagattgggt ctactagacc cactcccata aaagcgatca tacaggtagc tgctacaact
    56581 agcgctgaac ttttcccctt tgtttcttca tttgtttgtt cttgtatatc tatttgattc
    56641 tccaatattt ccatcccttt ctctctatta gcatcataca tgatttattt tacatgtcaa
    56701 tatgcacatg taaaacgtgg aaactttact gtgatataat aaagaaaata agacgagagg
    56761 taggattttt atgaatactt tagcctatat attattatgc gtgcttttat ccaaaccacg
    56821 ttctgggtat gaattgaagc agctgatcac aattttttgg gaagcccatc acagtcagat
    56881 ctatacaacg cttgccaagt tagaccagca gggttatgtg accgttttgg acaaagacga
    56941 gcactctcaa aaaaagatct accacttgac tgatgaagga cgtgctcttg tagaggaatg
    57001 gatcaaagag gaaactcccg ctcctacgca aaaagatgaa tttctagcaa aaatctatgc
    57061 tttttcgacg ctcgataaaa caacagctac tggactgcta ttcactagag aacaacagct
    57121 gaacaaaatc atgcaaaaaa atcaaaaaaa attgaatgac ctctcgctag acaagaaaca
    57181 agaatttgga cgttatgttg tgatccagcg cagaattatg ctttgtcagc aggaactcca
    57241 atggtgtcag caagtaagag aacaaatgca gcagtttttt gagtaaggga taaacagaac
    57301 ctttctagaa atatctattg aagagcaaat tatcttcaat aaaagagcaa tacagttgga
    57361 aaagaaaaag cgatccgaac gacaggccaa aacctatcgt tcggatcact atttttctag
    57421 aactgcattc aagagactat gacaaaaagc atgccaaggc cttttttcaa ataacagtgt
    57481 tccatcattc attactttga attgctttta gcacacttct tgaaagcggg ccaacgatga
    57541 tcaactgcaa cggtaaagcg gcaatcacat tcattttcca agcattcata taagcagaga
    57601 ataattgatc aggtattcct tgttccatga tcacaccgaa taaagacata catgtcacca
    57661 ttcctaaaac catcaagcat gaaatagtta agatcatttg gaccttgctt tctggattta
    57721 ctggtagttt aaaggcaatt tttttcgcca gcacaccgat gatgaagaca tccaaaatga
    57781 aagccaccac gaatccaggc actagaccaa tcaacaaact tgataaatga agatcttgat
    57841 gcagccataa attatacgcg ctcatcccca agaccatcaa aaaacacatc attgttgtaa
    57901 aaattattcc ttctttttta tttgtaggca atctgtacca ctcctttaaa tttgtcacga
    57961 aaatcatcgt agcacattta aaaatttctc gtaagtactt tttttatttt ctgccctttt
    58021 atttcataaa cttcatcagc cgtacgctcg acaaagtaag aatcatgaga agtaaacaaa
    58081 atcgttcccg gatatatttg cattagcttc tctaaagctt cgatcgttgc catatcaata
    58141 aaattagtcg gttcatctaa cagcaacaca ttacttggct tagtaaaaag caaagcaatc
    58201 gtcagacggg tcgcttctcc cccacttaat gtacataacg gtttcgtcaa agtttcctga
    58261 gcaaacccta agtgattcag caaactacgg acaagcgatt ctgaaaactc tgtttgtctc
    58321 atgacaaaac gaatgatgga ttcttcagat gtcatttgat aacccttttg ctgatatact
    58381 tgaaagctta ctttcggaga gagaaggatt ccttctcctt gtttgcggat atgttctaat
    58441 aaggttgtct tacctgaacc attttcaccg acaatcgcga tttttttacc taaagaaaac
    58501 tgaaaatcac catttaccag taatgttctc ccacttcttt ccaattggac attttgtccc
    58561 atgatgggat agcggctgtg gagttcaaga gattttggta ctggaaaacg gatttgtttc
    58621 atctgctgcg gcttggttgt ttctcctatt ttttggagtc ttctctctaa tgttttcgca
    58681 ttcttttgga tggctttttg tacactgtct ttttgtttag aggaagacaa ccgatccagt
    58741 ctgatttgtt gctttttctt tttactggac actttttcca acttttccgc ttgttttttc
    58801 ttttcttgga tcgatttttc caatcgtttt ttctctttca tgatcgactc tgcttctcgt
    58861 agctgagtct ttttctccaa ttccttttga gtgagatagg cactataatt ccccgaaaac
    58921 tctcggattt ctccgtcttt tacttcccag attttcgatg ctagctcatc tagaaaatag
    58981 cgatcatgac taacaaaaac cagcgtacca taataataac gaagctcttc aatcagtcga
    59041 tcaatacttt ttctatctag atgggttgtt ggctcgtcta gaagcatccc ttgttcataa
    59101 gaagaaagaa gacgtatcaa ctcgatcttt cgttcttccc ctccgctcca tttccgctct
    59161 gaagtttctt ccagtcttaa ctggaaatga ctggctaatt ccaaatttag gtcctttgct
    59221 tcagcgagat aggttaattg aggcaggtag ttgaaggtga tctctgtttg gattttccca
    59281 tgatcgggaa aaagttcacc ggctattagt ttgagcaaag tcgatttacc gacaccattt
    59341 tctcctatga tccctatacg atcttgctga tagacacgaa gagaatcaat agatagttct
    59401 agctggttgc caaagctgac ttgtagattt gttatattta ctgctaaatt ttccatatgt
    59461 tttacactcc tatttcatgt aaaacaacaa ttatcgatac taaaaaagag cagaaaaatc
    59521 tgtttgcaaa aaaactgcat cacatctttt cctactctga acgaaataac tatgactaaa
    59581 tcaccaaatc tgtcatagct ttcacctatt ctactgtcgt tcgaaaaaat ggacatactc
    59641 catccatgaa attgttgttt atacatgtct tttgaattta tttagttgtt attcaaaagt
    59701 tcgaaacgta atttcatcga tgcagtcatt tttccgacct ccttttttat taggttacca
    59761 cacacgattc aaaaaaagca atagctactg aatagtaact gaaaaccttt tattcgaaca
    59821 tagtgatctt ttctggtgct aaacgaacag actcgtaacc ttcttctacc gcttttccta
    59881 ttgataaaat aataactgga acataacggt ctttatccaa accaaatgcc tctgccaact
    59941 gatctgcttc gaatccacca attggatttg tttcatagcc atgatcacgc gcaactaaca
    60001 tgaattgcat agccgctaag ctagaatcga ctttgactac atcgttcatt tcttctcttg
    60061 aaaaactctt gtaataagga atgatcgcag ctaattgttg atctctgact tcttttggca
    60121 tcttccctga ttcgtaagct tggttgtaga tttcttcgcc atattcgtaa cattgcatat
    60181 cgccaaaaat catgaccatt gcagaagaag tatcattttg tctcgtattg aaacggatca
    60241 aaggacgcaa gatttctttt gcttcctcgc tttcagcgac cacaaagcgc catggtttca
    60301 tattgacaga agaaggtgca gtcgtcgctt ctttgatcat ttccagcatt tcttcgtgtg
    60361 ggattttgta tgtttcatca tatacgcgaa cggatcttct accagtcata attgttgaaa
    60421 aatcattttt ttgttccatt attattaacc tctttcgttt ctgttgttat attgcaaagt
    60481 tgggtattca atttatctaa aagtgttaga aattgtttca tttctacatc gctgaaagcc
    60541 ggggatagca cggaacctgt atctttatgg tccatttcac aatgcttgat tttcttctgg
    60601 gctttttctg ttagcgctac atacacttcc cggttgttct gaggatttct ctgacgcgaa
    60661 acatagccat ttgcttccaa taccttcaga tgacgagtaa ctgctccttg atcaatctgc
    60721 aacttttctt gcaaactgct ttgtagcaat ggattttctt ctttcagtct catcatgatc
    60781 tcgtatctag taagacttat accgatattt tcttcaaaga tctggacgat tttttgatcg
    60841 gctaatttga tttgatataa aaggtgacta atctcttcta aagacataat ttcctccttt
    60901 ctaataattg actcgtcaac gattgacttg tcaataatag aataaaaaaa gaaaaaagtc
    60961 aaagaatcag ctgtgtaata aaaaaaacgc tctcaataga aaagtataga gaaaagaaat
    61021 tttttgtaga atgaacggaa aaaatgataa agaaagcaca acgctaaaac caagattatt
    61081 gattcttatc aaactataga agatagctct tctgcagtag ataattttac accgccaaaa
    61141 atttccgaaa tcgccttttg caagaatccc gtcgaatatt agaaattgga tcagtatgct
    61201 taaacagtaa taaaaaagtt atgacaagat gttgtcacaa ccttttttga gtaaacggtg
    61261 ttcaaaaact tgcatctgcg gtaataagtt caaccaaaca aaaaacatga aaatctgttt
    61321 ttgtttggct gttcttacta cttgatgcag ggcagctttt tcactacctc tttttaataa
    61381 ccataaaatc ctattccttc atatctccag cccatcggtg ttagccaatc tctttcctta
    61441 gcactcaagg tatagtgatg ggtaccagtt ttttcatttg gattaaatag tcgataaatt
    61501 ggcttggaac caccagaata aaaacttaga ccttcataac gccaaccgat ttttgttaaa
    61561 gagttgatct cagattgtgc cattgtgtaa tgatggtctc cactgtaagg attgtataaa
    61621 cggtagacag gttgtccgct gtttggtgct tgccaagcaa tcccttcata ttcccagcct
    61681 acagttggca gataattttt ttctccggca ttcaatgtat ataaatgttc tcctgtatga
    61741 cggttataca gacgatacat cggagaagtt gaagttggtg ctgtatcata ctgtgtcaga
    61801 ttataggttt cgattacatt gttcaatgta gaaccataat taggcgcagt ggcataacga
    61861 cctgttagcc aagccgtagc atctttgtaa gagttcgtat tgcttttcca tgcaccggaa
    61921 tagtagtaaa cgcctggctg aaaagaagtc gtcttcaata ccgttacgtt atcttgcaat
    61981 gattctttgt acgaaggata ttttctaaaa gcggcattca ccgtaatcca ttgcccattc
    62041 acatattccg aagtaggcat cgtaacagat tggccattat agcttccttt aatcccaaat
    62101 agattgtaat taggggcaga agccaaagta ctgttccccc agccactttc aacgatggct
    62161 tgagcaatca tgactgatgc atataagtca ttggcacttg ccaaactttg tgcagttgag
    62221 gcaatactat caatgaacgc ttgttgagaa ttagcagatg agcgagcata aaaagcatag
    62281 ccagtatagt tttcgaattc agacaaatct gcttgatggg tatgtgtcaa tgtttgccca
    62341 tctgtcaatt ggttcgtgat ttcttctgca ttctctggat tgttctgagc ttctgctgat
    62401 gtactgtctt cggtttgtgt ttgttctgtt gtttcagttg ttggttcttc ggttgaaatt
    62461 gtattttctg agacagtagc tgactgcaaa gtactttctg tatcgttttc aattcctgtt
    62521 tctgaagaac tcgatgtctg agttgcgaca gtttctgtcg attcttcagc tagcactgtt
    62581 gtgttggcaa ctgctagatt tcctaaaaga ataacgctaa ataataaatt ctttgctttc
    62641 atcttttttc tcctttgaat tatgaaactt ttcctaaata acagtgtaac taaaaaagta
    62701 aaagcaagtc aatgtccata tttaaataac atttgtttct ttttaataaa tttgcttttt
    62761 ctgcataaaa agacgtaaat gaagaatatt cttctccatt cacgccttaa attgatggtc
    62821 gtttgaataa ctatttaaca agtaattctc tctggatatt atccgttatg gattatactt
    62881 cctgtgtcgt cggacggata atcatgttgc tgacagacat ccggtctggc gtttctatcg
    62941 caaatgctac agcagaagct atatcttcag atgttaatcc ccattctttt tgcgcttcat
    63001 gaagttcaag agcagccttt ttgtcgctga tggtttgata taattcagtt tgaacagctc
    63061 ctggagagat gatcgtagat ttgatgttat tttgccgttg ctcttgacgt aatccttcca
    63121 tgattgcacg gacagcaaat tttgttccgc aataaacggc ggaatcagga taaacaacat
    63181 gtcccgccac agaatcagtt gaaatgatat gcccagattt ttgtttttcc atgattggaa
    63241 gtacggcgga aattccattt aacacaccca tgatgttgat atcaagcata ttttgccatt
    63301 catcacggtg accttcaact aatggagcgg ttggcatgat tcctgcatta ttgtacaata
    63361 catcgattcg accgaacttt tccatcgcta aatcgataac tgcttggact tgtgcaaaat
    63421 ctcttacatc tgctgtttga atatatagct cagcttctgg taaagattct tttattgctt
    63481 ttaggcgatc ttctcggcgt gcagctataa ctagtttagc ccctttttct gctaatagct
    63541 tcgttgttgc ttcaccgatt ccgcttgaag cgcccatgat gacaatcact ttttctgata
    63601 aatcattcat tctattctcc tcctgttttg aaaaatctct tgcttatact cactatctct
    63661 tatttcatct tactgttcaa ataatccgtt cgacaggttt gacgattttg gcagggattc
    63721 ctgcaacgat ggtattgtct gggacatctt tcgtaacagt tgcattcgct gcaacaatgg
    63781 cattctcgcc aatcgttacg cctggcagaa tagttgctcc tgctccgatc caagcatttt
    63841 tcttgatgtg gattggcttt actgacaagc ctcttcgttc ttttggatcg gttagatggt
    63901 tcaccgtgat gaggttgaca cgaggaccaa tcaatactga atcttctaaa cagatgccac
    63961 ctaagtcaac gaacatcact tgacgattga taaagacatt tttccctata ctaatatgtt
    64021 tgccgaaatc tgtataaaaa ggcagcagaa cagtcaccgt atcatctact ttgtcactaa
    64081 tgatattttc tagccgtttg cggatatctg ctggttcatg gtacccagta ttcagcgctg
    64141 ctaaaggctc ttcgttttcc gcttggatct gatggatcac ttcaaagagc ggatccgtta
    64201 ctcgaatctc tttattgacg atttgttttt cgatgaggct tgtcattttg ctccctcctt
    64261 ttatccttac tttataacgg acggcttatg atgtctaata cttataaaac atacttaact
    64321 attcctttta actatactta gtggtatgct tttttaaaaa caggagggaa aaaatggata
    64381 ttcgtatact gacgtatttt ttagcagtag ctagagaaaa aaatatcagc cgagcagcaa
    64441 aatctctgca tatcacgcaa cctactctct caaaacaact gaaagattta gaagaagaac
    64501 ttggtgtgac acttttcact cgaggctccc gagagataca gttgacggat tctggtcgat
    64561 atctttattc aaaaggaaaa gaaatcttgt cgttggtaga aaaaacggcg aacaatttaa
    64621 caaatgacgc aatcatctct ggagaaatct acattggcgg aggcgaaact aaagcaatct
    64681 ctttcatttc tgatagcctt cattcgttga tcaaggcgca tcctgatatc cagtttcatt
    64741 tatatagcgg gaatgcagat gatatcatag acaaactgga tagcggatta cttgattttg
    64801 gactcgtaat cgaaccgaca aacaaacaaa aataccagtc atttcgactg ccagcccaag
    64861 atcgttgggg acttttgata catcggaatc atcctttagc aaagcaaaca gtcatccatc
    64921 cgcaggaact gaaaactgtt cctttgatca tttcccggca aacgtcagtg gacagtcaac
    64981 tagctgaatg gctaggtttc agcatagatc agttatcgat cgtcggaaca tataacttat
    65041 tgtacaatgc ttctttgatg gtcgataacg ggacttttgg tgcattagct ctagatggga
    65101 tcatcaatac ttatgggacg gaaaaagtat tcatcccact cgatccgcca ttacaagctt
    65161 cactgaatct catttggaaa aaagaccagc ctctttctcc tgctgctcgt aaatttttag
    65221 atgtcctaac ggagaaatac agttaagtag agtttttttc cttcgctttc agctttttaa
    65281 caacgaataa gttaagcaaa agatccaatg attaaataac ataagtttct taatttgttc
    65341 cttcctttta tttctgataa ttccagtaaa ttactataat tataaaaaaa gaattgacaa
    65401 aacataactg tctatataaa attgattacg ttaacaataa ttcgtttcat gtgactagat
    65461 catactaggc atgaaaaatc tctgaggaac atggtttccc taggaggttt ttcgtgtcta
    65521 tttttgtttg gccgcaatca actggtctag caaacgtgaa aaccaaatct tcaaaaaggg
    65581 tgcgcgcatg aaaatcaaaa agatctttaa tcaaaatgca gtattggtag atgacggtgg
    65641 acaggaaaaa gtagctattg gaaaagggat tggatttgac agaaaaagaa atgatttgat
    65701 ttttgatcgt gatgtcgaac ggatatttgt catggaacca gaaggtcaaa tcaagttgca
    65761 aaatttactt aatcagattg atgaacgctt tttatttgca gcagaaacga tcattgatca
    65821 tgcggaaact gttttgatgg aaaagttgaa tgaacatgta ttgatcgctt tgaccgacca
    65881 cattgctttt tctgcggaga acattaataa cggtatcgtc atccgcaaca aacttttaag
    65941 agaaatcgaa gtgctttaca gtgatgaatt cagtattgct caatgggcag tagagtatct
    66001 gacaaaagag cttgggattc catatactta tgacgaagca ggatatatcg caatccatat
    66061 tcacagcgga cgaagcggtc ggaacaataa ccatcgcagt atccgtgaag tgacgattgt
    66121 atctgacatc atccatttag tggaatcaga attggaaaca gatatccatt cagaaccatt
    66181 ttcactgaat tattcaagac tggtgaatca tttacgactg cttctacaac gaacccatgc
    66241 gcaacaatat gctgttttag acacggaaat cgtagaaatg gtcaaacgaa aatacccaga
    66301 aagctataag atttctaaaa aaatccgtgt gttgctgacc aaagaatacc agctcgcgat
    66361 cacgaaagaa gaactgggtt acttggccat ccatatcgag cgtttaagaa gcgcaatcgt
    66421 acaaacaaat gttaacaatc atgaggagga aaaaaattga aagcatatct acaacgaatg
    66481 ggacgttccc ttcaacttcc agttgcggtc ttacctgctg ctgctttact agtagggatc
    66541 ggaaactggt gggcatctta tagtaacgac attgtcgctc attttttaca agctggcggt
    66601 aacgcagttt taggtcaatt acctttgctg tttgccgttg ggttagcact agggatgtca
    66661 gtggacaaat caggtgctgc tgccttggca ggtttggtcg catttgaagt tccagtaaat
    66721 gttttaaaaa ctgattcagt agctacttta ttgaatatca aaccagaatc agttgatcca
    66781 tcttttgctc agatcagtaa cgtatttatt ggtattattg ccggattgat tgcggcagct
    66841 ctttataatc gtttccacga agcaaaatta ccgatggctt tatctttctt tagcggcaag
    66901 cgtctagtgc caatcttggc tgcctttgtc atgttgctga tcagtgcagt gctactgctc
    66961 gtatggcctc cggtttactc tgctttagtt tcttttgcta aattcatttt gagtttaggc
    67021 tggggtggtg caggtcttta tggcttcttc aaccgtttac tgatccctac aggattgcat
    67081 caagcaatga actccgtgtt ctggtttgac gtagcaggaa tcaatgatat tggtaaattt
    67141 ttggcttctg aaggaacaaa aggcgtaaca ggaatgtatc aagctggatt tttcccaatc
    67201 atgatgttcg ggcttccagc aggagcatat gctatttaca gaaatgcacg tccagaaaac
    67261 aaagcaaaaa cagcttcctt gatgttagca gcagcttttg cttcattctt tactggtgta
    67321 acagaaccac ttgaattctc atttatgttc gttgcttggc cattatatgt cttgcatgca
    67381 attttcactg ggatttcttt agcagtgagt gcattcttcc attggacagc aggatttgcc
    67441 tttagtgccg gatttgtcga ttacttccta tctttgaaaa atccgattgc gaatcagcca
    67501 ttgatgttga tcgtccaagg attagtaaca gcagtgattt actactttgg ctttaacttt
    67561 gctatcaaaa aattccatct gatgactcct ggccgcgagg aagcagatct ttcagatgac
    67621 gatactgcaa cgactaattc agacaataaa tatgcggcac aagcaagcaa aatctacgct
    67681 gcattaggcg gagccgacaa tgtaacttcg atcgataact gtactacacg ccttcgtttg
    67741 caagtcaaag atacaggcct gatcgatcaa aataaaatca aagccactgg cgtacctggt
    67801 atgaagatca tcgacggaaa aaatgcgcaa gtcatcgtag gtacagaagt acaattcgtt
    67861 gcagatgaga tggcaaaact acatggtggt gctgcaacac gacctgcaca aacaaacacc
    67921 gtggttaaaa cagaaacatt tgccggtgga acgatcgaac aagatattta cgctatcgca
    67981 aatgggaaac tgattccaat cactgaagtt tcagatgatg tattcgcgga aaaaatgatg
    68041 ggtgacggct atgctgtatt accagaaaat ggagaaatct tctctccaat cgctggtacg
    68101 atcacaaaca ttttcccaac gaaacatgct gtcggaatcc aaacagatgc tggtatcgaa
    68161 gtcttgcttc atatgggcat caacactgtt gacttaaaag gcgaaccatt cactttatat
    68221 gtaaaagaag gtcaaaaagt cgcaagaggt caattgattg ctttagttga tcttgccgct
    68281 attcaatctg caggcaaaaa cacggatatg gtcgttgtat tcacaaatgg cgacaaagtc
    68341 caaagcttag aaatcgaacc cgcacgtgat gtaaaagcaa atgataaaat cggctctgtt
    68401 tccaacaaag cctgagccat cgtcttcccc agactttttt atcgataaac aaacaggagc
    68461 tgagacaaaa atcttgtctc agctcctttt tccgaataaa aacggcgttc tcctgccaac
    68521 tccgcggtaa taagtccagc caaacgtaac atatgaagag ctatttttta tttgactctt
    68581 cttattactt gatgcagaac agctttttta caacctctga taaacggtgt tcaaaaacca
    68641 gtttatcggt atgaagtcaa atttatgcaa aaatgtggaa agccattttc acaaaatttt
    68701 tcttcatact caaaactaat cggctttttc acaacctctt tatacattat tcctgatcca
    68761 atatttcttt taatgctgct tcaagagaag ttaatggttt tcccaatact ttttctaaat
    68821 catcagagga aatagctaac tgatcctttt taatatcatg ttggatcgct aaaaacatgt
    68881 cagctgcttc ttgagggaat ccggcttctt ttagattttc gacaaacgct gcatcacttg
    68941 cttcaataat ttctaacttt ttacctgtcg cacgctctaa tgccttggct aattcttcat
    69001 agtcagttag attgccggat aattctaaga tttctggaga gtcactttct actagtactt
    69061 ttgctgcagc ttctgcatat tcacgtttca atacccaacc tgtttttcca tttcctgcag
    69121 cataaacgaa ttgaccagtt tttaaagcct gcccaatgat aggtaattca ttctctacat
    69181 accaattatt ccgtaaaaat gtatgggaaa tacctgaatc cttaatcatt ttttcagtga
    69241 attggtgatc tggcgctaat acactgatcg agtgatcggc atctgcaaaa cttgtataag
    69301 cgatataaga taccccagct gcttgtgctg ccttcactac attcttatgt tcggcttgac
    69361 gatttcctgg aacgccagag acaaaaagca atcgatctat tcctttcaac gcttctttca
    69421 ttgagtctaa atcatcataa tcaccaatac gtatattaaa tccagcttct ttcagtgctt
    69481 ctcctttttc ttcactacgt actagtacat agatttcatt ttgtggaacc atctttttta
    69541 agaattccag cgcataacca ccaaaacctc cagttgctcc tgttactaaa taatgcatat
    69601 ttgccactcc ctattctttt tatttgttaa gatttacagc tgtacattta taaattacac
    69661 ctttatcata aagagtgtgg taagataagt caagcaaaaa aaatgaaagg atgacaactg
    69721 atgaagtatt ctctgcaatt tagtgatgca atccatgtac ttgcctatat tgaaatgttt
    69781 caagataccc gatcactttc tagtgagatg attgccagta gtatcgaaac aaatgcagca
    69841 aatgtacgta aaatcatgag tcagctcaaa aaatccggtt tgatctttac aaaaaccgga
    69901 aaaccgcagc cttcccttgc caaaagtcca gatcagattt ctttactaga tgtctatcgc
    69961 agcatcgaag gtaatacaca gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc
    70021 gttggtgcga atatccaaga agctttagaa gaaagctacg ctcggctgca gaaaaaggca
    70081 gaagaagaaa tggctaagat cacacttgat agtctgatac ataaaatagc cgttttggaa
    70141 aaagaaaaaa gaccagaaaa cacaggcctc gtagagagat ttttataatt ctctctactg
    70201 tgcctgcatt tttctgatcg gaatcgtcta cataaattct tttattctcc gggggacaaa
    70261 agccttgtca tgcccctttt tcctctctaa tgctcaggtc aaagcgctga agtcatgacg
    70321 tcttgataaa cggtgttcaa gtagctgacc ctcggcatta gttctgaaaa atgcaaaata
    70381 tggaaagcta tttcccattt ttcctctcta atgctcaggt caaagcgcta ctttcacgac
    70441 ctctatatta tcatggttta ttgactaccg gtatccagat ttctcccata tatttcccgt
    70501 tggtaactcc catttcgact acagcatttg gacccccaac ataggcgtaa tcttctactt
    70561 ctcttaaaac atgcccaaag gccgtattcg tcaaatcatt tcttaattct tcaggcgatt
    70621 ctgctaaagc gattacaaca agatacgttc cttttgggaa tgggatgatt cgggtagtgt
    70681 ttggaacatc ctgactcgtt tgaacaccga catagtacat cattttcccg ttatagggtt
    70741 cgttgactgt aaagaaataa tgattttcag cagcttgttt caatgcatct attgttccat
    70801 taccgataac tttttgctca aatgcttgtt tctctgcagc caaaccgaca aaatccgtat
    70861 tgctactctt caattccaca ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc
    70921 tatactttgc catttctccc accctttcta ttatattaag ttcatcttac ataaaattaa
    70981 agggaaatga cacgattacg ctcttttttc tagggatatt tttatttttt aactattttt
    71041 ttgctttcac tgcaatccaa atctcactgt aaacattact gaaatcctct ccccattttg
    71101 taaaagagat ctcgggagct tcgactaact cataatctga agaaggaagc cattctgcat
    71161 agattttccc ccaagtatct tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg
    71221 cccaagtgtg ttcttctata gcaagcattt ctaaatcagc gtacggattt tcttttgtcg
    71281 tagcaaagcc aatcatgtgc atcaaactgc ccttttcttc caagcgggct ccatcaaatc
    71341 cataagaaac gttgattact tgatttggat ataaatctcc taacttatgc atctcttccc
    71401 tttgagtctc cgtgatcgtc tgcgctaact tttgtatttc ttgattgact ccttcaaatt
    71461 tgacaggaac ttttttcgcc acacctgcca cataaaactg ttctttcttt tcgatacgaa
    71521 attccatcga ttttcctcct ctgacatcaa taaaaaatgt caagcgtgga aaagattttt
    71581 gtatgccgta tctgctgatt tctgaaggca gatatcctga ccaatcacga aaagctcttg
    71641 aaaaaccttc gactgtttca tatccatatc taaacgccgt ttctgtcact cttgatccat
    71701 ctaggagatc gcgattagcc aaagccaatc tacggttttt gatatagtcc attagcgaca
    71761 tccctgcaat aaacgtaaag atgcgtttta attgatattc agagattcct gtattttcgg
    71821 ctattatttt ataggaaagt ccttctgtca aatgttcttc gatgtaatcg atcaattggt
    71881 tcagatcttt aagcatattt atcttcctcc ttgcttgcct ttttatcata ttatcaaaga
    71941 aagtcaatta cttgatgaat caggaaccaa tttagtcaga tgtagaaaat gttgtatgga
    72001 tctaaagaaa attctcaagc agcgtttcgt aacggataat tggtattatc tgcctatcta
    72061 tgttttacct ttagaaaaat aaattttggg gaagacacat tctaagtaaa caaagcggta
    72121 aaaatatctt ttattccctt agttaatccg cttactacct actgtacaaa gcaaattgtt
    72181 gttcaaactg ctgatcagaa cgctgcttgg atttttcttt gcgatacatc atacctcttt
    72241 tatcaattgg aacaattatt tatttctaac aaaatcactt catttttgtc agataaataa
    72301 aaaacaaaaa aagttccatc tttaaaacac gctattcttt tactttttct cacattctct
    72361 aagaaaagaa aaggagaatg aatttttgac aattcgagca aaagtgatta tcaaatatgt
    72421 aaaatcagct acttttttct taaacgtttc tttatactaa atataaataa gtgtttcatg
    72481 aaaggagaac tagatgaata attggcttga gtattttcct gaaaacgttt tggaacgcgg
    72541 ttacagctat catctgcacg gttttgtccg tcatttgaac tatacaagca agtacttgtc
    72601 cgcaacagtt tctggcacgg aagattataa ggtagtgatc acttgggatg aaaagaccaa
    72661 catgacttgt gattgtctat atgccataga aggaaaaaaa tgcaaacaca tggctgctgt
    72721 cttattcgct tacgaagaac gtccaataaa aaaatccaac tactcacttt ccgaactctc
    72781 gtcccttgtt tcctccgcta gttcctctct tgtacgggag cttttaacag agattcttat
    72841 agaacatccg caatttatcg aacgcttcaa agttaagatg ccatttcatg caataaatta
    72901 ttcagataaa ttaacaacta tcatccataa atacgatcat attatcaaga aaaacaaaaa
    72961 caggaaaaca gcgaagttca tcatggagat gcgaaagttc atccaagaag cagttgaatc
    73021 gctcattcag caaaatgcct atcttcccgc attcgaattg atcaatgaag tgattgctac
    73081 tctcgaaaca ttctattggg agcctgagga tgaacgaacc cttcttctga tagaggactg
    73141 ttattattta tggaaagaat tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt
    73201 ctcttggttc gtctgccaag tcgatcatac tgacgcctct tattcaaaac gctactccat
    73261 aaaaatcctg aaagaagact ttcgggaaaa agaattttct aaccaaaaga aaaaaataga
    73321 taaaaaaact aaagaaacgg taaagaaaga cgatttcaat gaaaaatagc aagtaaacga
    73381 taacaaccat cctaaatcca caagaaatac agcagacctt gctttaaaaa cacgaatcat
    73441 gcaaaggatg aagtaaagaa gacttaacgc gtacccaata ttagtcattt gactattagg
    73501 tacgcgtttt gttttataag tcatgacaaa tttccttcgt tgctgggtag attttccggc
    73561 attgttgata gattttatga tacttttcta cattttgtaa gagaggttcc actgcatctg
    73621 tatattggat aaaattctga caacaacttt caggtgtgtc gaaccactct aatctttaaa
    73681 gataaatatt aaaataacga ttaataatat taacagtctt tatttttcac caccaaaaac
    73741 ctatctttgc atatactaaa tagacgtata tacacagata ggtttgtctt gtgaaaaata
    73801 atgattatcc ttaccaaaaa atgtttgttt ttctacatgt tcgtacttat caaaaaatat
    73861 gatccttatt gaaattactg gctatttata ttccaacgac ttcttgtgtt aatttttgtt
    73921 atctcatagt aaattatgtt tcattttcac ttaatttagt tgtcacgtgt tcgtcaattc
    73981 gtaaaaacgg taaatatata aacacacttg cggctataat taatatttgt aaaacagctg
    74041 ctcgccagtc tccggctgtt gctaaaaatc ccgatataac tggtggcgta gtccaaggaa
    74101 tttgcactac tacatgatta attaatccca cagcagttgc aaaatatgct actgtcaaag
    74161 aaaaaatcgg tgcaagcaca aagggaataa tcagactgat attaaataca ataggcaatc
    74221 caaaaataat aggttcgctt atattgaata tagcgggagt taccgataat ttagcaaagt
    74281 ccttataatc ttttctacga ctgaagataa atattgcaat caataaagca atcgtatttc
    74341 ccgatccacc cattaccgca aaggtatctt taaaggccat attgataata tgtggaattt
    74401 gctctttgtt tgcataagct gccatatttt cttgcatatt ctgaatcaaa aacggatcta
    74461 acaatgaacc tgatattact gcttgatgga taccaaatcc aaaaaataga ttagcaatag
    74521 atgtaattaa caaaaatcct ggtaaaccag ttgtaatttt acttaatggc tctgtaacta
    74581 tcgtggtaat tagcttattt atatccatat taaacaattg aatgattata aatgacaata
    74641 aagcaaacga ggtaatcgta ataatgattg ggattaatac attaaaagac tttaaaacag
    74701 cgggaggaat atttccggat agctgaatct gtagtttctt attattagaa agtttaataa
    74761 ataattctgt agccgctaat ccaacaaata tacctacaaa cattcctgaa gctcctgtat
    74821 aggatatagg tatcacaccc gtcacttcaa cagctttatc cgcattttca ggagtaaaca
    74881 tcatctgcat aggtgttaat acaacaaatg tcgaaatagc aactaatacc gctgcaatat
    74941 gatttgtata atttctgctt gcacacatat gataagatac tgctgctaca actaataatg
    75001 tagttatgct taatgtacca ttggcaattg aagtaccaat ttgttgtaac gtggtcaatg
    75061 tactagtatc gacaatttcc cccatgaatc ctgttggttc taagatcact gaattaataa
    75121 gtgtcacaaa tcctgctaac atcataaatg gcataaatgt agcaaacgca tctcgcaaag
    75181 ttcttaaatg gatctgattc cccaacttag tcgcaactat accaaatttt tcaaatacaa
    75241 tgtccttcct gctacttttg cccatatttt ttctccttta tgcttattgt aatcgattac
    75301 tttttccacc caaataatac atcacttatc aaaaaaagta aatactttta
//
LOCUS       DAZKOU010000003        68136 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000003 DAZKOU010000000
VERSION     DAZKOU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.003"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(225..2411)
                     /locus_tag="ACG827_000143"
     CDS             complement(225..2411)
                     /locus_tag="ACG827_000143"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0482318.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2629..3312
                     /locus_tag="ACG827_000144"
     CDS             2629..3312
                     /locus_tag="ACG827_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0482319.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3393..4112)
                     /locus_tag="ACG827_000145"
     CDS             complement(3393..4112)
                     /locus_tag="ACG827_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0482320.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4424..4630)
                     /locus_tag="ACG827_000146"
     CDS             complement(4424..4630)
                     /locus_tag="ACG827_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482321.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4875..5477
                     /locus_tag="ACG827_000147"
     CDS             4875..5477
                     /locus_tag="ACG827_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0482322.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5618..6898
                     /locus_tag="ACG827_000148"
     CDS             5618..6898
                     /locus_tag="ACG827_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0482323.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6911..7639
                     /locus_tag="ACG827_000149"
     CDS             6911..7639
                     /locus_tag="ACG827_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0482324.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7708..9231)
                     /locus_tag="ACG827_000150"
     CDS             complement(7708..9231)
                     /locus_tag="ACG827_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0482325.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9483..10664
                     /locus_tag="ACG827_000151"
     CDS             9483..10664
                     /locus_tag="ACG827_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0482326.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10724..12352)
                     /gene="treC"
                     /locus_tag="ACG827_000152"
     CDS             complement(10724..12352)
                     /gene="treC"
                     /locus_tag="ACG827_000152"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0482327.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12803..15514)
                     /locus_tag="ACG827_000153"
     CDS             complement(12803..15514)
                     /locus_tag="ACG827_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0482328.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15524..16225)
                     /locus_tag="ACG827_000154"
     CDS             complement(15524..16225)
                     /locus_tag="ACG827_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482329.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16411..17010
                     /locus_tag="ACG827_000155"
     CDS             16411..17010
                     /locus_tag="ACG827_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0482330.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17091..18104
                     /locus_tag="ACG827_000156"
     CDS             17091..18104
                     /locus_tag="ACG827_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0482331.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18212..19129
                     /gene="rbsK"
                     /locus_tag="ACG827_000157"
     CDS             18212..19129
                     /gene="rbsK"
                     /locus_tag="ACG827_000157"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0482332.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19157..19552
                     /gene="rbsD"
                     /locus_tag="ACG827_000158"
     CDS             19157..19552
                     /gene="rbsD"
                     /locus_tag="ACG827_000158"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0482333.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19552..20925
                     /locus_tag="ACG827_000159"
     CDS             19552..20925
                     /locus_tag="ACG827_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0482334.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21282..23426
                     /locus_tag="ACG827_000160"
     CDS             21282..23426
                     /locus_tag="ACG827_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0482335.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23478..24518)
                     /locus_tag="ACG827_000161"
     CDS             complement(23478..24518)
                     /locus_tag="ACG827_000161"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0482336.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24795..26597)
                     /locus_tag="ACG827_000162"
     CDS             complement(24795..26597)
                     /locus_tag="ACG827_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0482337.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26611..27363)
                     /locus_tag="ACG827_000163"
     CDS             complement(26611..27363)
                     /locus_tag="ACG827_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482338.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27372..27734)
                     /locus_tag="ACG827_000164"
     CDS             complement(27372..27734)
                     /locus_tag="ACG827_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0482339.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27910..28782)
                     /locus_tag="ACG827_000165"
     CDS             complement(27910..28782)
                     /locus_tag="ACG827_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0482340.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28833..29321)
                     /locus_tag="ACG827_000166"
     CDS             complement(28833..29321)
                     /locus_tag="ACG827_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0482341.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29459..30250)
                     /locus_tag="ACG827_000167"
     CDS             complement(29459..30250)
                     /locus_tag="ACG827_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0482342.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30305..30913)
                     /locus_tag="ACG827_000168"
     CDS             complement(30305..30913)
                     /locus_tag="ACG827_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0482343.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30906..31223)
                     /locus_tag="ACG827_000169"
     CDS             complement(30906..31223)
                     /locus_tag="ACG827_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0482344.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31379..32107)
                     /locus_tag="ACG827_000170"
     CDS             complement(31379..32107)
                     /locus_tag="ACG827_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0482345.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32266..33147
                     /locus_tag="ACG827_000171"
     CDS             32266..33147
                     /locus_tag="ACG827_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0482346.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33384..34427)
                     /locus_tag="ACG827_000172"
     CDS             complement(33384..34427)
                     /locus_tag="ACG827_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0482347.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34430..35098)
                     /locus_tag="ACG827_000173"
     CDS             complement(34430..35098)
                     /locus_tag="ACG827_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0482348.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35240..37576
                     /locus_tag="ACG827_000174"
     CDS             35240..37576
                     /locus_tag="ACG827_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0482349.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37691..38002)
                     /locus_tag="ACG827_000175"
     CDS             complement(37691..38002)
                     /locus_tag="ACG827_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482350.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38183..39658
                     /locus_tag="ACG827_000176"
     CDS             38183..39658
                     /locus_tag="ACG827_000176"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0482351.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39705..40289
                     /locus_tag="ACG827_000177"
     CDS             39705..40289
                     /locus_tag="ACG827_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0482352.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40291..40710
                     /locus_tag="ACG827_000178"
     CDS             40291..40710
                     /locus_tag="ACG827_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0482353.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40991..41275)
                     /locus_tag="ACG827_000179"
     CDS             complement(40991..41275)
                     /locus_tag="ACG827_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0482354.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41627..42337)
                     /locus_tag="ACG827_000180"
     CDS             complement(41627..42337)
                     /locus_tag="ACG827_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0482355.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42334..43806)
                     /locus_tag="ACG827_000181"
     CDS             complement(42334..43806)
                     /locus_tag="ACG827_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0482356.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43944..44417
                     /locus_tag="ACG827_000182"
     CDS             43944..44417
                     /locus_tag="ACG827_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0482357.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44570..45121)
                     /locus_tag="ACG827_000183"
     CDS             complement(44570..45121)
                     /locus_tag="ACG827_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0482358.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45226..45756
                     /locus_tag="ACG827_000184"
     CDS             45226..45756
                     /locus_tag="ACG827_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0482359.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46239..46682)
                     /locus_tag="ACG827_000185"
     CDS             complement(46239..46682)
                     /locus_tag="ACG827_000185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0482360.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46698..47858)
                     /locus_tag="ACG827_000186"
     CDS             complement(46698..47858)
                     /locus_tag="ACG827_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0482361.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48365..50065
                     /locus_tag="ACG827_000187"
     CDS             48365..50065
                     /locus_tag="ACG827_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482362.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50167..51000)
                     /locus_tag="ACG827_000188"
     CDS             complement(50167..51000)
                     /locus_tag="ACG827_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0482363.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51304..53469)
                     /locus_tag="ACG827_000189"
     CDS             complement(51304..53469)
                     /locus_tag="ACG827_000189"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0482364.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53729..56023
                     /locus_tag="ACG827_000190"
     CDS             53729..56023
                     /locus_tag="ACG827_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0482365.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            56016..56681
                     /gene="pgmB"
                     /locus_tag="ACG827_000191"
     CDS             56016..56681
                     /gene="pgmB"
                     /locus_tag="ACG827_000191"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0482366.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56674..57681
                     /locus_tag="ACG827_000192"
     CDS             56674..57681
                     /locus_tag="ACG827_000192"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0482367.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57703..58722
                     /locus_tag="ACG827_000193"
     CDS             57703..58722
                     /locus_tag="ACG827_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0482368.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(59003..60370)
                     /gene="nhaC"
                     /locus_tag="ACG827_000194"
     CDS             complement(59003..60370)
                     /gene="nhaC"
                     /locus_tag="ACG827_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0482369.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60479..61900)
                     /gene="tyrP"
                     /locus_tag="ACG827_000195"
     CDS             complement(60479..61900)
                     /gene="tyrP"
                     /locus_tag="ACG827_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0482370.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(62143..64020)
                     /gene="tdc"
                     /locus_tag="ACG827_000196"
     CDS             complement(62143..64020)
                     /gene="tdc"
                     /locus_tag="ACG827_000196"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0482371.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64311..65567)
                     /gene="tyrS"
                     /locus_tag="ACG827_000197"
     CDS             complement(64311..65567)
                     /gene="tyrS"
                     /locus_tag="ACG827_000197"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0482372.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65679..65884)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            66147..67448
                     /locus_tag="ACG827_000198"
     CDS             66147..67448
                     /locus_tag="ACG827_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0482373.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67517..68116)
                     /locus_tag="ACG827_000199"
     CDS             complement(67517..68116)
                     /locus_tag="ACG827_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0482374.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        20432 a        13818 c        12126 g        21760 t
ORIGIN      
        1 atttttctat atattctcta ttttgataat agtattctca ttttcaaaat aaataaggga
       61 ggtgtgacaa aattcttgtc acacctccct tttccgaata aactgtgttc cagaaatagc
      121 ttcttcgaaa ataagctgaa atttcacaaa aatttgagaa gcaattttcg tacattcctt
      181 cttctttctt gaaactgacc acttctgtca caccctctct tctatcactt tccttttttc
      241 acacgactcc cggtttcttt gataaatctc gatactttca attttcttga atttttcatt
      301 tgaggtacac atagatacag acgatttctt gctctagtca tcgcaacata aaatagtctt
      361 cgttcttctt ctatcgtcgt tactggttct gcttttattt cattaaagtc gattactttc
      421 ttttctgtcg ctccgttata aggcaaactt ccttcgttac aaccaatcaa aaagacattt
      481 cggtaactaa gacctttact tttgtgaatg ctctggatac atagtgcatc aagttttcct
      541 tgtctgtaag caatcaagcg ggtttttaat tctttagctg tcgcttgggt ttcttgcgat
      601 aaggcttgga gtcttctgaa agtagcatgc tgctttactt cttctcgtat ccattcttga
      661 tcactctggt cgatttcctt agctatacgt ggcagatgaa gatagctttg aacaagcgaa
      721 tagatttctt gatgctggtc tgttttacgc aacgcttcct caaactctcg gatttctgca
      781 gatttcgatt ttaaattggg gcttatcgac aaaatataat ctgcaacggc tatattttga
      841 tatttaggag aaaagtattg atcgcattca ctttttttca ggtagcagtc tgccatcagc
      901 tgccggagca attgctggta ggcctgattt ttcttttgca gattcagtgc tgtttgccaa
      961 tgaacaattt tttgatagaa atgttggaaa gacttgatac caaagatacc ttcatatgga
     1021 ctatctgctt ccagtacaaa aggaatattc gttttcagca gagattggac aagctgctgc
     1081 gtttgtgaag ctgatcggta taatatgatt gattccttat atttcccatt tttttgaata
     1141 aatgtctgaa tttgtttgat tagccactga ctttctgctt tagtatcaga aaacaaatag
     1201 gcttccataa cacctgcttc tttttgtgct ggaatcatcg cctttggtat ccgatcattt
     1261 ttattatagc tgatcaatct atttgctcct tggacaatag ctgcccgaga acgataatta
     1321 gttaataaga aaagagtttt ggtaggatat tctttttcaa agttcttgat gtatgctggc
     1381 tctgatcctc tgaatccata gatactctga tcatcatctc caacaacaat caacttgatc
     1441 ctgttttcct ttgtcatccg tttcacgatt tcccattgaa gtggattagt atcttgaaat
     1501 tcatcaatga atagataatc atatttttgg ccgatataat gctggatctc ctcattctcc
     1561 aaagcgcttt tcagattttg gagtacatca ttgaactcta gcaattgctg gcttcttttt
     1621 tgttcttgga gttccgtgag caatccatac agtcgttctg atatcggctg tcgtgcattc
     1681 ttatcaatgg aatcatattt ttctttaact aactgaacca tttcttctac tgaaaggttc
     1741 atattctgcc aatagtctaa ccgagaagca atcgtttttt ctccatattg atcaaatata
     1801 taaagattat atttacgtat cagctgttgc aaaacagcat tcactttttg tgcagtatcc
     1861 tttgaaaagt ctccttgtat tccttgcatt gctacttcag gaaattttcg acaaatttca
     1921 ttgtatatcc taaaaaagat actatggaat gttccgatat ggacagcggt ttttctctgg
     1981 ctgaaattat ctactcgttt ttttagttca tttgcagcgt tccttgtaaa agtgatcatt
     2041 agtatgcgtt cttcgtctac ttgtttttgc gtcatcagat aaacagcttt tgcacagatt
     2101 gtttgcgttt tgccgctccc agctcctgct atgattcgaa gatgtttctt catatcatat
     2161 gtgatagctg ctttttgttc ttgattgaag cgattatagg aacgtttctc catcatttta
     2221 aagaaagagc tgtttccttc actttctttt acttgtactt cttcctcaat aagtccgggt
     2281 gcttcgccat aagccgcttc ataggtagca atgatttctt cttgtagccg aaagacttgt
     2341 tgaagctttt tatacttgtt ctcccagcgt ttcaattctt tttgtaatcg gagaatttct
     2401 ttttcattca actgattgat ttccttccca tctttcttct ttgaatttgt ttatttcaag
     2461 ttggttattt aaagtttcgt tgtctatttt ttctttatac taacagacct ttctaaaaat
     2521 gaaaatggga agcgatatga ttttagtttt ctgaaaattt ttcctttttt tcaatcgttt
     2581 atgctaagat aaggaagaca aaaatattca taaaaggtga tgaaaaaaat gcagacttcc
     2641 tttcacgagg ggcgtacaat aggacttttg ctgacattta tcggaggagc catggacagt
     2701 tatacttata tccaatatgg tgcttttgcc tctgctcaaa ctggcaatat cattctatcg
     2761 atcatccaag catttgacgg agaatggcta agtgttggta aaaaagtatt gtctaccctt
     2821 ttctttttct tagggattct tctaacaaag tatctaattg attattttag aaaaaaagaa
     2881 aaacatttct ggcgcttgtt tgttctatat tatgaagcag tgattttttt ctttgttagt
     2941 ttgacacccg tccatgagca tcccgcatta gtaacgattt tgattgcttt tactgcatcg
     3001 atccagtggg tagcttttga taaaatcaac gggcgtgcct acactaattt atttacaact
     3061 ggaaatttaa aaggggtagc aacgaactta tatgattatt gtatttctaa agaaaaagca
     3121 gattttgatc gatttttcca ctatctaagt gtcgttcttg cctttatcgc aggagctatt
     3181 gtccttgttt tctgtcatca tctgtttggc gcccattcga ttttgatcgt ttccatttta
     3241 tttttgatca tggccctcac tcagacattt catttatggc aattttatca tacgaatcgg
     3301 ttcactattt gatctcaatg aatcaaaaaa actaagcaag atgctgctca cagagaaata
     3361 tctgcgagtg agcaatttgc ttagttttta tattatttag ttgtaaagat tccctttgct
     3421 ttcgggttat ttgtagtgta gcggatcgac tgggcatctg cctcttctgt cattttcttt
     3481 gcccgcatct tgatttcttg tacgatttgt tctccacatg ccggatcggc tacgaaatca
     3541 tatttgtccc catcgatcac tattttggga catatatcaa actgttcata ccactcttcg
     3601 tatcgtcgat tgagttcttt atagtaggaa tagagttcag gatcttgttc tagctgttca
     3661 tattcccggc ctcttttttt gattctcgct aacatagttt caaaggatac acgaatataa
     3721 accagtagat ctggtttctt ttgtggcgaa atttcttcta actccttcag catcgtttct
     3781 aaaagacctt catactgctt tacttctact tcagaaacac gccctaaatc tgcattcaaa
     3841 tgaaacagca tactatcttc gtagatggag cggtctagta cgttgtcatc atggctgaaa
     3901 gcatctttga ttgccaaaaa gcgtttgttc aaaaagaaaa tctgtaataa aaaagtatac
     3961 ttttctggat tagaataaaa tagcggcaat acttcattat cttccacgtt ctcataaaaa
     4021 ggttttgttt ctaaatcttg ggcaagcatt tcagttagtg tgcttttccc tgcaccgatc
     4081 gttcctgcaa caatgatcac aattatcggc atccttcctg taataaaatc tgttcttcat
     4141 tttactactt catttataga aataaaagcc ttgactttat ctgctcctta aaagctactt
     4201 ctctaaaagt agccaaaagt aactcctatt catgaaaata aaggctgtga caaaagtctt
     4261 gtcgcagccc ttttttcaaa taaacagtat tctcctgcca acacctcggt cacaaggcaa
     4321 tattcataaa acatgaaaag ctgtttttta acattattcc ttgtaactca gagctaaacg
     4381 ggagactcac aacctcttta tgctctcaac attttatctg tttttattct tctattgtac
     4441 ttgatttgcg tacaactaac tgccagtgca gattatgagc ggtatcattg tcttctactg
     4501 cgcatttcat gacacgtatc atcacactat tttcatagat tttttctatt tttcctttta
     4561 ttggtgattc aaataaatca gatttaaatg catatgtttc gccgacttca aacaattgtt
     4621 cattttccat aagaatccct ccatcacctt ttataaggtc agtaattttt taacataaat
     4681 cactcgaaaa agcacaaact tgtcataaaa agtacttttc gtatcaaaac tgtcactttt
     4741 tttggtaagg ttcttggaaa acgaagattt ttttaggaga tcatgacggt tttgatgtca
     4801 aaaccacttt ttatgaaaca aaagacgttt tttatttttt ttagcataaa atttgtggtg
     4861 gaggtacgta tgagatgaaa gataaagttg tgaacaaatt attagaagcc ttattcagcg
     4921 gagacaaatc ggaagaagat atggtgtatg tccaagtaaa atttgtctta gaagttttat
     4981 tgaataatct tggaaaacta gcggtcgttc tagtcttttc tctggtgaca ggctcatggg
     5041 cggaaacagg aatcaccttt ctttcttata tctgcatccg cagatatgct tatggattgc
     5101 attccgactc tgaattcgtt tgtctgctgt ggacactact ttatttatgg ggcgttccct
     5161 tggtgatgaa acatctgcaa ttgactatct ccttcccaat gatggtcttt ttgttaatca
     5221 gctgcttttt gttactgctt cgctatggat caagaggtac agcaatcaat cccattgaac
     5281 ccgagaaacg tcccccatta ttaaagaagg cgatcagcat gtttctaatt ttttctctca
     5341 ttacgttatt tttctcagct tcttactttt ctacctattt gctacttggg attgttttag
     5401 aaatagctac gttgcttcct attacaaact acttgatgaa ttttggagga atctttcatg
     5461 aaaaaaacaa tcattaaaca attaactaac ttatctgact tttttgtatc tggctgctgc
     5521 tttggtatga gttctgaatt caaaatccct gaaaaattaa aaaaataata cgccaattgt
     5581 gctataattt ggtaaaaatc aatgggagct gtactttatg tttttatggt ccatagcgtt
     5641 gcaatatttg atttacacaa tttgtatctt attgattgac catcacatcc gatacaaata
     5701 tctgtttgcg ggagtcattc tgatttcttg tattttgatt ggatgggtct attcgatttt
     5761 agggattttt actgctctaa ttgctatagg aatgctgatg cttacttttt tcttcttttc
     5821 gacttcgcgt caacaattgg tttacgcgct tccttttggg ttattgacgt cgcttttagg
     5881 agatcactta acaagcatcg gcgatttttt tgttttgaaa tcttcggtta ttgaaccagg
     5941 agattcattg cagtattttc acattgtctt atccgctttt ttatctgctt tattagcttt
     6001 ttgtttaaaa aaatcgttag agacttggtt tcacactgtg gaccgccgta tgatcggtac
     6061 gatcgggaca ttgatattat ttacatacta tatcattatt ttttataccc gtttttctgg
     6121 tgaaacacct gatgtcttag ctttgaatac gagttttttc attctatatc tgtgtgttgg
     6181 cctgatcatc ttggtttatt attggaaaat ctctaataag aaattagctg atcagttatt
     6241 agagcaacaa gtcaggatgc aacaggatta tatcaaagat ctagaaaaaa actatcaaga
     6301 cttgagagaa ttcaaacatg attaccaaaa tctgcttttt tctcttcaca gctatatttc
     6361 agaaggcgac ttagaaggat tacggtctta ttatgatcaa acgatattac ctacacgcca
     6421 gatgatgaac ctttttccgg ccaatctaag tatgttggat aatatgaaag ttcctgagat
     6481 ccgtagttta ctgtcattaa aactcatgat ggcacaggaa aaagggttga tcgtccagct
     6541 tcattttcct gagccaatcg aactggatac gaaacatacg gtaaaccttg tacgtatgct
     6601 tggtattatc ttggacaatg cgattgaagg agctagtgct gcaaagaaga aaaaaatcga
     6661 attgaccata ctcaagaaga atcattccgt gatgattcgt gtgatcaata caacaacaaa
     6721 tacattaccg ttaaaccagt tgaaacaaaa agggttctca acaaaaacaa atccaaaaaa
     6781 cgaaggcctt ggcttgttta tcttggatga attgacaaag accaatcctt atcttttcct
     6841 tgaaacatca attgaaaacc agcgtttttc tcaaacgatt tatatacaaa taaaataaag
     6901 gagtaatgat atgattccca tttatatttg tgaagacgat ccaaaacaat tagaagtttt
     6961 atcaacggtg atcaaaaata gaatcatgat tgaaaactta gacagttatg tccatatttc
     7021 tacctctgat ccggaagaac ttctcaatgc agccaagatc agaacttctt cttttggtat
     7081 ttacttttta gatatcgaac tagaagacag tgaaatggat ggtgtagcga tcggtaaatt
     7141 gatccgtgaa ttagatcctt taggaaaaat aatctatgtg acttcccata cggaaatgtc
     7201 gatgaaaatc ttaaaaagca acatcgaacc gactgattat atcgtcaaag aagatcttta
     7261 tgatttaaaa gaaaatgtcg agcatatcct gagcaaaatt ttcgaagatt ttcaaatgtc
     7321 ccaaatccaa aaagatatct tcaaaataga gtttaatgat gagatcaaat ttttaccgat
     7381 caaagacatc caatattttt ctacttcgct aggagcgcca cataaactgg aagtccattt
     7441 gactcatgcc caattacaat tctatgagaa gatcaaagaa atcgagaaaa tgcatcggaa
     7501 ttttatccgt tgtcataaat cctatgttat caatacacaa aatgtgcggt cgattaataa
     7561 aaaaaccaga gaagtcacgt tggcaaacgg cgagaccatc ccagcctcta tccgtggatt
     7621 aaagaaattg atttcataaa aagaaccgaa tagtttggac cgactaaatg accagccgat
     7681 ccaaaccctt cggttctttt ttgcctatca gtgcaaaagc atttcctgtg caatttcacg
     7741 tccatccatt gtatctggat aaaatgttgg ccaattctcc atttctttca gcaatacttc
     7801 ttgactttca tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg
     7861 atcactgaat tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata
     7921 ccttacgcca cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt
     7981 tgaacgtttc gttgttccat tcttaaacgt aaatgccatc tctttctcac tgatcttgat
     8041 tgaagcaata tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt
     8101 atcctgattc aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata
     8161 cgtatctact gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc
     8221 gtcttcaaaa taaccgttgt agacgctctt ttcttttttg ggggtcaaag tatcttctag
     8281 cgggttttct gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag
     8341 atagttttct ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc
     8401 taaagataca cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc
     8461 aaaataaata tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc
     8521 cgcagacggt tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc
     8581 taacgctaga tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt
     8641 aaaggtttct tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt
     8701 ttttgtccat acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc
     8761 tacctgcttg attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg
     8821 gccttcttcc ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag
     8881 cataccttct gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc
     8941 ggggacccat gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt
     9001 tgccatgtct ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa
     9061 ctctacttct ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt
     9121 cgtcatcacg ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt
     9181 taacatacct gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac
     9241 tcctcttctc atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt
     9301 tacgttttag caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct
     9361 tattttaatt gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct
     9421 atcattttcc ttttataccc gcttttgtct ataatgaatt tatagataaa ggaggaaaaa
     9481 gagtgaaaaa agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct
     9541 cttactatgt tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga
     9601 tctcgtttat attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt
     9661 ttgcactgat cttgtattat cttttggaac cagtcatcaa acgtttgagc aagcaccttt
     9721 cgaggaactt gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa
     9781 gcatatggct tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag
     9841 atttgtttca agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg
     9901 attcatttaa tcaatttttc gcttctattg acgacgtgac gtctgatata gctggattta
     9961 ttggaaatta ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag
    10021 ctatttttat tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc
    10081 aaaaatttta tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa
    10141 atttgacaaa gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt
    10201 catttatcct tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta
    10261 gtgttttagc tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg
    10321 ccttctttcc aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg
    10381 tcattgtctg gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg
    10441 gtgatcgttt acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc
    10501 taatgggaat tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg
    10561 ttacttttat tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag
    10621 agtatgaaaa ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat
    10681 cttacgagaa tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag
    10741 caattgcctg gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat
    10801 cgataagaat attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat
    10861 agaaatttgt taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt
    10921 ccggatgatt tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc
    10981 tcagccgaat caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt
    11041 ctacattaat ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc
    11101 ctgcttgttc tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt
    11161 tgatccgacg aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat
    11221 tcaaactttc tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga
    11281 tttcttctcc catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca
    11341 tctctgctcc ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct
    11401 gatcatgatt attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc
    11461 atgtatgaaa tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc
    11521 cattttcata atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac
    11581 taggttctgt atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga
    11641 tgatagtgga atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga
    11701 ttggtttgtc cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt
    11761 ctttaccgat aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa
    11821 aacgaacaac tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt
    11881 gtgtccgatc atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac
    11941 ttccaccaaa ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat
    12001 aatgctggtc ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca
    12061 gcaccatatc gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga
    12121 attcctccat cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt
    12181 tgtctttttg agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat
    12241 tcagatacgg gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt
    12301 ctttgtaaga tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt
    12361 acctccattc aactgattta ttacctacca caacttatat atacaagtta tcatttatta
    12421 ttataactga accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg
    12481 cccaataaaa aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa
    12541 atattcttgt ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc
    12601 tcaaatgtaa aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac
    12661 ggctttttta caacctctga ttcatggtgt tcaatcagct gtccttcggt attagctcaa
    12721 aaaacggcaa aatatgaaaa actattttgc cgtttgtttc tctaatactc aggtcaaacc
    12781 gctgatttca caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat
    12841 gctttaattt gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg
    12901 ctgataagac ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg
    12961 cttgttgcaa gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta
    13021 aaagagtaaa gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc
    13081 caaggacttt tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat
    13141 tatacaagac gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt
    13201 ctaagttcgc cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg
    13261 tcacagcgtc tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct
    13321 tatctgtacg cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat
    13381 attcagggga catataaata aagctaccta gatagttttt cacgatttct tttacttcga
    13441 ctttccattc tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa
    13501 atagttctgc tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct
    13561 cttcaagatc agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca
    13621 ctgtcagact ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag
    13681 ctaggatctc atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat
    13741 cttccgtgga tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt
    13801 tggctgaaac agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac
    13861 atccagcaat tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt
    13921 aactgatttt ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa
    13981 tgcgtttccc tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat
    14041 ctttgaataa aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt
    14101 gtaaaatcgg cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag
    14161 acagctcaaa aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac
    14221 ctatccctga ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat
    14281 aacctaacgc ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg
    14341 tcgtaaaggt gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg
    14401 cagcgatacg atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat
    14461 tgtataggta tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt
    14521 cctctgcaga ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt
    14581 gagcttcttg ttgagataat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct
    14641 ggatagttgc tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt
    14701 gcgttgtttg tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg
    14761 ccgatttttt cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct
    14821 tttcttgatt ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta
    14881 cgtttgtaaa atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta
    14941 aatagacaaa ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt
    15001 tgatgaacaa tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat
    15061 ctgaatcaat ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca
    15121 atacaagctg gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct
    15181 tgttattttt gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat
    15241 agatctgttt tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt
    15301 tttgtgtcaa gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat
    15361 agtgatccat cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg
    15421 ttcctagcat gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc
    15481 gaaaacctgc tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat
    15541 caatggatga aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa
    15601 tgactctatc agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc
    15661 cgtgttgtct gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca
    15721 gcgcgcctgt cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa
    15781 ttgaaaccct ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag
    15841 ctaggcccac ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg
    15901 ccaattccac attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga
    15961 accctacatc tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg
    16021 tgtcatcaat aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa
    16081 tcgttgactt tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg
    16141 aaaaagaaat atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt
    16201 cattttttac ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat
    16261 atctatgcat cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac
    16321 taaagaaagg aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa
    16381 aaaacattgt tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt
    16441 gatttatcaa aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt
    16501 acactctctc cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca
    16561 ttgaaacgta ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat
    16621 ttgagtcaac aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat
    16681 ttacttttga aggattcttt gaaagataca gataatatgt tgactatcac aaaatacaat
    16741 ccaagtgctt tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact
    16801 atcattttaa gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt
    16861 caacacgggt accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc
    16921 catcaattgt tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa
    16981 ataatcgaag ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc
    17041 tttttgattt catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa
    17101 aaaagatcac aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct
    17161 tgatattaaa tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg
    17221 ccaaagaaga attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa
    17281 cgaaaacaat tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc
    17341 gaggaattga ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt
    17401 ctgatcatca aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat
    17461 tcattatcgc gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag
    17521 gccggccctt cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa
    17581 cagacgattt tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa
    17641 cgatcgccct tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt
    17701 ttaaatctgc tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac
    17761 agttctcaaa acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg
    17821 gtgtgtttgc tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg
    17881 gaaaaagcat ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt
    17941 atatcaagcc gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg
    18001 ccaagttatt gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac
    18061 ctgtacgatt agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta
    18121 tatttttatc gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc
    18181 aaaataaatg agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat
    18241 taatatggac actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc
    18301 gcatgagatt tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg
    18361 ttcaggcgca cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt
    18421 attagatcta ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc
    18481 cacaaccgga caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca
    18541 tgcaggggca aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac
    18601 aaacagtgac tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt
    18661 ttctattgca aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac
    18721 gattcctact gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga
    18781 aatcattaca ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact
    18841 ccatgaatta ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca
    18901 tacagaaaaa gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc
    18961 cgcaggtgat acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt
    19021 gaaagaagcg atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc
    19081 gcaaccttct atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg
    19141 agaggaagaa acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat
    19201 cgcacaaatg gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt
    19261 tggtactgaa aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt
    19321 agaaaacgtt ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac
    19381 aatgaacccg gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt
    19441 tatcccacat gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg
    19501 cgaaatgaca ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg
    19561 acaatgaatc ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg
    19621 ggaggaaaag acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg
    19681 aaaaaacagc cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat
    19741 ttcattttac tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac
    19801 aaggaattga cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa
    19861 aaactatttg gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct
    19921 ggtatattaa aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc
    19981 ggaacttatc atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt
    20041 cttgcttttt ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct
    20101 gcagcactga ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact
    20161 gattttctgg ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt
    20221 ttaggtattt tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat
    20281 atgcaattag tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg
    20341 gtattcgggc ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg
    20401 atggatatta acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta
    20461 atatttggta tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg
    20521 ttcgaccgaa tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta
    20581 ggtgtatttc tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct
    20641 tcgattttac ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa
    20701 aaaccatttg ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc
    20761 caagtgaaag cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc
    20821 ggtacttttt tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact
    20881 actctcgtta gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact
    20941 gaagtatgag atgtttatca catatttcag tctttttctt tttaggatca ttatttttat
    21001 aaatttttgt gatactttcc aaactttggt ataatttaca tatgcataac catttttatc
    21061 cccacttgtt tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa
    21121 cgaatgaatg ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta
    21181 tgaaaagaaa aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga
    21241 ataagacaga aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga
    21301 tacgtttgct agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat
    21361 ttgccgtaga taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct
    21421 cagctactag tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa
    21481 caacaggatc gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc
    21541 cagccgagac atattccctt acaattgctc cttctttgat tggtaagatc caactgactg
    21601 cttcgacagc ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag
    21661 aaggtttatt agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa
    21721 ttgcttcagt cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag
    21781 taggtacgaa caatttatct attactgcag aatttaaagc tggaccggat acttcaacag
    21841 agaaaccaac tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca
    21901 aaccagatgt gggtaataac aacaataata atggttcaaa taattcaaac aataataaca
    21961 ataattcgaa cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg
    22021 gaaatactca gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca
    22081 atgcttcatc tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt
    22141 cttctgattt tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg
    22201 tccaacaagc aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag
    22261 caaaaggtcc aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa
    22321 ccggtcatta tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag
    22381 taagtcaggc ccaacctggc gacctcgtct tctggggacc ttcttccggt gttactcacc
    22441 atgtaggtat ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag
    22501 ttcgagtaac ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag
    22561 gtttacctgc tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg
    22621 gctcgttcca tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg
    22681 atgcacagga aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta
    22741 tccttgaaag tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg
    22801 gtatcaaagg atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt
    22861 ccggacaaag ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt
    22921 tggaagacta tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac
    22981 caacttggaa gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt
    23041 atgctacaga tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga
    23101 caaaatatga tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc
    23161 tcaatatgtc taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca
    23221 caggattaga attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg
    23281 cttcaatctg ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc
    23341 gtacagttaa agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct
    23401 ggcttgatct gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac
    23461 tcccttttta atttattcta tttctttata tttattttgt attccgttac aaactcgcgt
    23521 acttggttag gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt
    23581 tcttgagtct caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt
    23641 ccatcttctc caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg
    23701 atcgatactt tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt
    23761 ttcaacaaaa atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca
    23821 aagacctcac ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact
    23881 acttctcctg taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa
    23941 agtatatgtt cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt
    24001 gtaggattga atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca
    24061 tcgttcgtca gcgtataccg tacttctaca tctaaagttc caggaaatcc gttttccata
    24121 tctgggctag tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa
    24181 tgccactgct tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg
    24241 tctgttttta cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca
    24301 gccatacgtc cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca
    24361 tcaaaaccta agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc
    24421 cgtgccccaa gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag
    24481 tgatagtttt cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt
    24541 tcatttgcac tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt
    24601 aacgagaaaa aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa
    24661 tattgtcaca acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata
    24721 agcccaacca aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat
    24781 gcaggacagc tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc
    24841 agtacatagt agatgaaata caatacgaag aatatcgtaa acggaaccca aatatctttg
    24901 tatggatttt gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct
    24961 aagattcctg gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac
    25021 ccgcgtcgtg ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct
    25081 gacaagatct tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag
    25141 aatcccataa attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc
    25201 tggttgctgt actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt
    25261 gtttcgattg gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat
    25321 tgtggcaagt tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc
    25381 tgttccggca attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta
    25441 gcaggaactt tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc
    25501 aatggttgag ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga
    25561 gtataagaag cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga
    25621 ggattattca tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt
    25681 ttaaaaccta atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt
    25741 gagagcattt gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc
    25801 aattttttca atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca
    25861 gaatggaaaa tgaaataagt tcctaatacg atcgtaacta aggctacccc tataccaaag
    25921 acttgatagg tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc
    25981 agtactactt caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc
    26041 gcacgaatca atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct
    26101 aataaaaaca aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt
    26161 gtaaaatgcg aaatattgag atctaactga tccactaata atcggtggac gatacttgtc
    26221 aatcctacac cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca
    26281 gaaaaaatca tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag
    26341 tctttttgcc gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg
    26401 atccctagta aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta
    26461 cttttcaaaa aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc
    26521 ataactaatc ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt
    26581 gataatttcc aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag
    26641 tatttctcga tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc
    26701 gccatccttg atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac
    26761 aagaagaatc gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg
    26821 ggcacttttg gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt
    26881 gatcaatgca cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata
    26941 gctttccaaa atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat
    27001 ttcttttggt ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa
    27061 gctttctaat aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc
    27121 tagttgattt ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt
    27181 tggtttatct aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc
    27241 catgatccca ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt
    27301 ctttttctca ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt
    27361 catttctttc cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt
    27421 tcgacttctt catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct
    27481 cctttacgaa gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct
    27541 ttcgtatagc caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt
    27601 gaaactgttt ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact
    27661 acgtaatctg cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc
    27721 agaagttttt tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc
    27781 gaacaattct gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa
    27841 acaactaaaa aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca
    27901 aaaagtatgt cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt
    27961 gtctgtaatg attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa
    28021 attgatttct tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc
    28081 ttcaacaatg gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc
    28141 actagcgatt tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc
    28201 tgctactgct aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt
    28261 tcctcgaagc gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg
    28321 ccgaatggct gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa
    28381 tgcctttgct gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc
    28441 atccaaatca ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt
    28501 cattttttgt gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata
    28561 ttgtccaaaa aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa
    28621 caacaataac gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg
    28681 ccacatacct aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat
    28741 ccctgcataa ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct
    28801 ttatccttca ataaatatct aatgttactt ttttaatttt tttttcgaat acgcgccatt
    28861 acaccttcta gttctaactc tttgtcttca gtgatgtacc aaccgcttgc ctgtgtccct
    28921 ccggctgcct gcggtaaata atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa
    28981 tagtcttcta ccaagacctc tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca
    29041 gatttaaaga taaatcgttc cttcttccca tcatacttca atattcctgt atcgataatc
    29101 ggtaaagaat aataatccag ctctttcttc accgtcaata aatgtttgaa aacttcatgg
    29161 tacacttgct ctaattggcg accttctgaa ttgttcgggt aattaaccat cactttttct
    29221 ttgtctggat catagaaggt ttggcttagt tgagtgatat aagtatgtaa tctttccatt
    29281 tctgttttta acttttctct ttgaccttct tctaccttca tatactcttt caccctgttt
    29341 tctttttaga tattttcttt cttattttac tatagttatg tgcgttaagc gatgaaaaac
    29401 agcaaagatt ggaaggatct ttgctgtttt ttcttaaaat atccagttga gtacaggatc
    29461 aatctgtttc attgtcctct tgatccgtcg taatattgat cgatgtgtct ttagcagtga
    29521 tctttagctt tttctgtcct tgttctaaca aagtatttgt tattggctgg tagtcttttt
    29581 caatataaga ttcaccagac gtttggatag tatagccact gttttccatc tcatgcaaag
    29641 ataaattgcc accgtttagg atgacttttc cttctttgct gatcaaactg tcttctagat
    29701 ggaagttgct gttttcacct gtgacgatca agttatccac attggcctca tctaatcgaa
    29761 gtgagcctaa ttcatctttc gcagttattg ttcctcctgt aaattcattg aacgataccc
    29821 agccatcatg tgtcttagta ttcacttttt tgattgatac ttcgttgata tcaattgcac
    29881 tattattccc ttcgattgtc aattcagata attcatgatc tgcagggaca acaatagtga
    29941 tttgtgcact tgtgtcttct aaactaatga ttgcacctgt tgattttttg actgcattca
    30001 cagtcaacac gccatttttc tcctttattt ctggcttttc tgtcttatca taagtcgttt
    30061 ttatctcaaa atccaatccg cgtatgaccg aaactgtttg attttctgca tggatgacga
    30121 ttttgtttgc atccttgatt ttttttgtct cagtaaccat atcgatcaac cgaggaccat
    30181 tatcccaagt cagactcttt tgagcgccta atgtataagt tgcacctgct aggaggccac
    30241 cagtaagcat cagaccaata ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc
    30301 tccttcaaac gcccgctgaa tgcagatctc tttttgtaat aaaacgtcgt cccatccatt
    30361 taacaaagtt catcatgaca taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa
    30421 taaatactgc gcttcctgtg gcgaacagac cgacaccgat aaagaataat ccactcagaa
    30481 tcgattgtcc aaacacactg attcctgaga tgatcatgaa taggccaaca cctaagatac
    30541 tgatacataa aacatagata ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa
    30601 taaccaatat aaaccccagt gctagtggaa tgaggacagg agaagcaaat aaaccgataa
    30661 tgattaacca aatcaggcga atttgtcttt ttggattttt cactaactcg ttttctgctt
    30721 gttcttcttc tgaaaagtaa ctgattttta attttaaggc aaactgcttt ggtgtaccat
    30781 attcatcaat gatctgatct aatgtttttc cagaatctaa aaaatattcc tcataatact
    30841 ggatcaaatc aaaacgatcg tcttcaggaa tttcttgaag atgatccaca acttcattca
    30901 aatattcatt catttgtgct ctcctccaat aaccggtcta aagaatctcg aaaaacaatc
    30961 cattcatcaa taatttcttc taaatgacga cttcccattg gtgtcaacga gtaatatcga
    31021 cggtttctgc cttcaaaagg ttcatcatag gtctcaagat atccttcttt tttcaatctt
    31081 ctcaaaaccg gatatagtgt agattcactg acagagagac tttcttgtac gatttttgtt
    31141 aaggcatacc catacatatc ttccttgctt aatgctgcta gcacagtacc atcaagaaga
    31201 acagttggaa tctggatgtg cattacgtca ccttctattc tcaagtgtaa tattttattt
    31261 caatctatat tatacaacat ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa
    31321 ctgactaaaa gatttgatcc tttttagtca gcaaaaaatg gcttctcttt gatttttatt
    31381 ataacagtgg cataatccaa tttgtccaca tccagctcag tggcatgata acgatcattg
    31441 caggtatcat gtcagctact gggaacattt tcacgttgat cattctaaaa ccggtagcga
    31501 ccatcaagaa tccgccgcaa gctttaaaat ctaagatcat gtctggtgta gtcaatggat
    31561 aaataaattt tgccaataag aacaaaatat aaaaaataat gaattgggga attgctatca
    31621 cagaaacgac ataccctaaa ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag
    31681 atttagaaat caaaatcgta ctgtctcctg tcatcccaga atctaaactt ccatagatcc
    31741 ccgtaccact tgcacaaaat agaacaatga ccgtcactaa tgtgctgata aattcatcgt
    31801 gggtcatacc aagcttttca ctagggaaga ttttagaaat aggtacttgc atcagcgcag
    31861 cccccttatt tatcccatta cctaaatgaa caatcaaacc aatcgctgtt cctatcacta
    31921 aagcaaaaat aacagccggc atatttttca tcggcgcaat ggaatagatt cccatcccca
    31981 tcgaacacac accaaaaacc atcgtcagct gtgctttaaa atgttcactt aatttatttc
    32041 ctactaatcc gccaattagt ccgccaaaca aaacggaacc tacattgatc aaaactccag
    32101 ttggcatcaa aaaactcctc tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt
    32161 aattattgct ttcgctgcaa gaaagattgt aacagacaga gaagattaca ccaattcatt
    32221 ttttgtgtta aagtattctt tattggaata agtaggagga atatgatgga tatccataaa
    32281 ttcgaagttt ttcttgattt agccgaaaca atgaatttca ccaaaactgc tgatcgacaa
    32341 ttcaccaccc aaggaaatat ttctaaacag attctttcgt tagaaacaga attagacgtc
    32401 aaattattcg aacgcgccca tagaaaaatc gaattgaccg aagctggaag cttattgctc
    32461 ccatatgtaa aaaatgtcgt agaacaatac catgctatgc aagaagtgct tgaaacctat
    32521 acgaaagaca acaatctttc attaaatatc ttaacgatac cgacaatgat caattataaa
    32581 ggtttttcca aaatcaccga gtttttgaaa cgccatcctg aatttacagt ccagttgaaa
    32641 gaagtggaaa gtgtcgaatt aacttacaat gacgccaatg aacatagtga cacgatccac
    32701 tttgctagaa gtttccaggc cccaccaagt tcgattgagt ggttgcctac tgaaacagac
    32761 gattttgtag cagttctacc taaaaatcat cctttagcta gtaaaaagaa attggatcta
    32821 agcgaactga aaaaggagaa ctttttgctc cttggcccca ctacgaactt gtatcaacct
    32881 gtactagatc tgtgtcgcga agctggattt gaaccaaaaa tctcttataa aggagcaaga
    32941 atcgacttga tcatcaatat gattgcaaat gacatgggga ttgctattgt tatggaaaaa
    33001 acagtcaaga atctattaaa agaaaatact gtgattctcc ctatttctcc aacaacagag
    33061 agttatcttg catttactcg taaagtcggt gagcattctt tggcttcaga tacattttgg
    33121 gagtatttaa atcaagaaga aaactaagcc ctacattttc tgtccatcta tccacccaaa
    33181 aaaagagagc gacgcatgac tttcgctctc tttcttgcat catgtccttg tttcaatgac
    33241 acctctaaca tttctcgtag ttcaataaga aagcagcgat ttttatgcca aaagaaccgt
    33301 aagaacattt ccctaatcac aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat
    33361 ttttaggggt tttattcttt ttcctattgt ttacttaacc gtatcattgc tgtcgttcct
    33421 actccttcga tactgctata atcaatacgt ccatcatgca gttctagaat ttttttaacc
    33481 agtgaaagtc ctagcccatt cccagaaatt tgacgacttg tatctccttg ataaaatttt
    33541 tcaaacattc gatctctggt ctcttcattc attcctacac ctgaatcggt cacttccagt
    33601 acaatctctg tggctgtctc aaatagtttg atatgaatct gtccattgat ttgattgtac
    33661 tttatggcgt tatccatgat attcagccac acttggtaca aaaactcttc attgcctgtg
    33721 taatttactg cagcaagttc gatatccaat tctagctgtt ctttctccca tttaggctga
    33781 aaaaaaacga ttacttctct gatttgttca tctaatcgat actctttctt ttccagttgg
    33841 atacgctggt tttccagttt gttcaatttt aagatgttct ctgttaattg tgaaagctgc
    33901 gtgatacttt cgataatccg atataaaaat atttggcgtt cttcgtcaga gagatttggg
    33961 gcttgtaaca attgaacata tccttggatt gttgctagtg gtgttttgaa ttcatgggat
    34021 acactggaca caaagtcatt cctcagtgtc tcgatactat tgagttcttg gaccataacg
    34081 ttgaaatctt tatacagttg ctgtacttct gctactttct gctgttcatc cattcgaatc
    34141 gaaaaatctc ccgttgctac cagactcata tttgtcccta gcgcactgat tggatgcagg
    34201 atacgccgcc caacaaaaat cgatattcct gtacccacga ccatactgaa ccccgcaaag
    34261 acgaataacg ggtataacgt tggttttcca gccgattcag caggcgagat agaatgtcga
    34321 gacaataaga aaataaaacc gagaaaacac agcagtgtca ctaagatcga aatgaagaca
    34381 atactagcaa aatagatcca taattgtgaa aatagctttt ttttcatctt taaataaccg
    34441 ccttatagcc tagtccccgt actgtgatga tttgaaaatc ttccgtcttt tcaaaacggg
    34501 tccttaaacg tttgatatgt acatcgatcg ttcgttcttc tgtattcgta tctaatcccc
    34561 aaatatcatc catcaactgt tggcgagtga agatcttgtt gggataagac agtaatttat
    34621 acagaagcag aaattctttt tgaggcagaa tcaacgaatg ctgatcttgt aacactgtat
    34681 aagcatctag ctctaatttt gtttgtccga ttatcaagct actgctgtaa ttgatctttg
    34741 aacgtcgaag caatgcttcc acacgtagga tcatctcatt gacatcgatg ggttttgtca
    34801 tataatcatc tgctcctaat ttgaacccgc gtttcttatc ctcaaaatcg gcctttgccg
    34861 taatcattag aacaggtgtg tcaaatctgg tgctacgtaa tgagtctagc aagtcatagc
    34921 catccatttt cggcatcatg atatctgtaa tgattaattc tatacgtgtc gcttctagtt
    34981 ttttcagcgc atcctctcca ttcaatgcgg tgatgactga aaagcccact cgttccaaca
    35041 cagaacggta aagcaactgt aattgcggat catcttctac tactaaaata cgattcattg
    35101 atctccctct gttttttgaa aaatttctag attttttctt gtagttcatc ttcagttaac
    35161 atttatttcg tatactacga tcattgttct gatatactta ttagtatacc gaatatttca
    35221 atacgggaga agggtgatta tgcttcaatt aaaagatata aaaaaatatt ataaagtcgg
    35281 cgaaaccacc accaaagcat tggatggtgt gtctgtcgct ttcagacaaa aagaattcgt
    35341 agcgatttta ggtcctagtg gttctggtaa aacgactatg ctgaatgtga ttggtggact
    35401 agataattat gattccggtg atatggtaat caacggaaag tctactaaag actttaagga
    35461 cagtgactgg gatgcctacc gaaacaactc tatcggtttc atttttcaaa gttacaactt
    35521 gattggtcat ctaggcatta ttgaaaacgt tgaacttggg atgacattaa gtggtgtttc
    35581 taaagacgaa aaacgaaaac gggcagaaga tgcattgcac cgagtcggct tgacaaatca
    35641 tatgcataaa aagccaaatc agttatctgg tggacaaatg cagcgagtgg ccatcgctcg
    35701 agcactagcc aatgacccag atatcctttt gtgcgatgaa ccaaccggag cgttagatac
    35761 agaaacaagt atccagatca tggaattgat ccaagagtta tcgaaagaaa aattagtgat
    35821 catggtcaca cataatcctg aactggccca tcaatacgca gatcgaatca ttgaattttc
    35881 tgatggtaag atcttaacag attcccatcc tcacatcgaa cgaccaaaag atgatcaatt
    35941 taacttacgc agaacaaaga tgagtttttg gacagcgtta aagctttcct tcaacaatat
    36001 ccgaacaaaa aaaggacgta ctttcttgac ctcctttgct tccagtattg ggatcatcgg
    36061 aatcgcgatc gttctgtccc tttcttctgg attccaaaaa caaatcgata acacgcaagc
    36121 tgaaacgatg gcgaaatttc cgattactat ttctaaggta acgactaacc agactcgtga
    36181 cgatgccgga ttgggtgcaa gcaaagcgga ttatccagac agcaaaacga ttactgcaaa
    36241 agtcagcgaa gaagataaag cacagcacac gaataagatt gatcaaacat acgtcgatta
    36301 cgtaacagac atcgatccta acttaagtaa taatatcgga tttacccgta ctacaggaat
    36361 caatctttta cgtgatgtaa atggcaaagt acagcctgtc agcttctcga atcaaaatcc
    36421 tgatacggaa tctctttctt tatctagtac gatgtcagca atgacaggtg tgggagtgtc
    36481 aagtttccct acacagcttg acactagcaa agaaaacttt ttaaaagaca attattcttt
    36541 acttgccgga agttatccag cctcagcaac agatgtcgtg ctgatcgtag acggcaataa
    36601 taatactaat atcaacgcat tgaagaatct tggatttgat gtgaaagaag acgaaaaact
    36661 tgattttgat gatatcgttg gtacaacatt caaattagta aacaacaata cttactatac
    36721 aaaacttcca actgggaatt ttatccctaa tacagattat gatgcgatgt atcagaatgc
    36781 ttctgatgaa ttgaagatct cggggatcct acgcgtgaaa tcttcttcta caatgaatct
    36841 cttatctcca ggaatcgctt atagcgatca attaacgact caaatcgtca acgaaaacaa
    36901 agaatcagaa atcgtcaaag ctcaaagaga cagtggtgta aatgtcttaa ctactgaaaa
    36961 agttgacgaa aatgccaaac aaacattgct tagttattta ggtggagaca gcttgccttc
    37021 aagtatcatg atttatccga acaattttga agacaaagaa aagatcttgg attatctaga
    37081 tgattacaat aaaggcaaat cagatgaaga taaaatcatt tatactgatt tagctggaac
    37141 gatgactgaa ctgactggcg gattgatgga tgctatcact tatgtcctta ttgcttttgc
    37201 agggatctct ttagtaacaa gtatgatcat gatcagtatc attacttata catctgtgat
    37261 tgaacggaca aaagaaatcg gtgtactaaa agctttaggt gcacgtaaaa aagacatcac
    37321 tcgcgtattt gacgcagaaa cgtgtatttt aggtatttct tctgggatat taggtgtgtt
    37381 tattgcatgg ctagctacgt tccctatcaa tagcatctta tacaacatga cggatctaaa
    37441 aaatgttgct caactaaatc ctgtccatgc gattattcta gtgatcgtct ctactatatt
    37501 gacgatgctt ggcggtcatc ttcctgcacg aatggcagcg aaaaaagacg ccgctatcgc
    37561 tttgcgtgca gaataaggtt gtgacaacca catttagatt tgagtcataa gagcgctgaa
    37621 atagtagaaa acagcaagaa acgaggaagt aatatgttcc ttgtttcgcg ctgttttttt
    37681 taattatttt ttataattct ggtatacttt tctttccttt gtaccacaac tcttctggcg
    37741 tatacctgtc ttttccattt atgaatactg ttccatctgc ttcaatcgtt acttcagtcc
    37801 caatccacat attacgagaa agtgcgagaa gattttttct ttttcctctt cctttttttc
    37861 caaattgcaa ggcatcggtt atctcttctg tccaatgaga tttcacgtat tttacttctc
    37921 cgttttcaaa tgtaactttc aaaatattcg ctatattttc cacttttact gctttaggaa
    37981 atgactcctc catttttttc actttccttc ctccaacact aagtatgcgc tttcttttag
    38041 tgataagtat atcatagttt attctattat caaaaaaacg catccattcc attgtagaat
    38101 actttaccga acaagttgaa ttgttttcct ctttttcacc ttttactata gataggtaaa
    38161 gacaactaag gaggaatctc tcatgacaga tcaaccgtat gatttaagaa aagtcatcga
    38221 agaactaaaa acacttccag gtcaatatca cgaaacagat attgaaatcg atcctgaagc
    38281 agaattatct ggtgtttacc gttatatcgg tgctggcggt acagtaaaac gtccgactca
    38341 agaaggacca gctatgactt tcaacaatat caaaggtttc cccggcacac gtgtgaacat
    38401 cggaacaatg gctagtcgta aacgtgtagg tgaaattttg caccatgatt atcgaacact
    38461 tggtcgtttc ttgaaagacg ctgtagaaaa tccaatcaaa cctgtgaaag tagacaaagc
    38521 agacgctcca gcacaagaag tcgttcatct agctaccgat gcagacttcg atattcgcaa
    38581 cattttgcct gcacctacaa atactcctga agatgctggt ccatacatca caatgggtgt
    38641 agtcttagga tcagatccag aaaaaacaat gagcgatgta acgatccatc gtatggttct
    38701 agaagataaa gatacgatcg gtatgtacat catgcctggc ggacgccaca ttggtcactt
    38761 ccaaaaacaa ttcgaagcct tgaataaacc aatgcctatc actatcaata ttggtttaga
    38821 tccagcaatt gccattggca cgactttcga accacctacc actccacttg gctacaatga
    38881 actatggtgt gcaggagctt tgcgtaacga acctattcaa atcgttgatg gtatagcagt
    38941 cgatgaagtg ggtattgctc gtgctgaatt catcattgaa gcagaaatca tgccaaatga
    39001 aacaatgcaa gaagatatca acacgaactc aggaaaagca atgccagaat ttcctggcta
    39061 tactggggat gctaatccag ctgtacaagt agtgaaagtc aaagcagtta ctcaccgaaa
    39121 agacaatcca atcatgcaaa caacgattgg tcccagtgaa gaacacgtat ctatggccgg
    39181 tattcctaca gaagcaagta ttcttgcctt ggtcgataaa gctattccag gaaaagtagt
    39241 aaatgtctac aatcctcctg caggtggtgg taaattgatg accatcatgc aaatccgtaa
    39301 agaaaacgaa gcggatgaag gtattcagcg tcaagcagct attcttgctt tatcagcctt
    39361 caaagaattg aagacagtca ttttagtcga tgaagatgtt gatattttcg acatgaacga
    39421 tgtagtttgg actttcaaca ctcgtttcca aggagataaa gatatcatcg tgatcccagg
    39481 aatgaggaac catccattgg acccatcaga attaccagaa tataatcctg gctctattcg
    39541 tttccgtggg ataagtgcaa aaactatctt tgacggtaca gttcctttcg atatgaaaga
    39601 acattttgta cgtgcaaaat tccaagaagt agaagattgg gaaaaatatt tgaaataatg
    39661 aatgatctgc aactgttttg acatcataaa aaggagtgcg gaaaatggaa caattggcac
    39721 aacggaagaa aaaaatcgtg attggtgtaa gtggcgcttc aggcaccatt tatgccatca
    39781 acctagtgaa gaaactaaaa gaatatccga tgatagaaac acacggaatc atcagtccat
    39841 gggcaaaaca gaacttaaag ctggaatccg acttatcact aactgaattc gaaagcctgt
    39901 ttgattacca ttattcaaac aaagatttag gtgctgctgt cgccagcgga tcatttttga
    39961 ccgatggaat gatcatcgta ccggcaagta tgaaaacagt cgcaggtatc tctgttggtt
    40021 taggcgacaa cctgattgca cgtgctgctg atgtaacaat gaaggagcaa cgaaagttag
    40081 tcattgttcc tcgtgaaacg ccattaaatg ccattcactt ggaaaacttg actaaattag
    40141 cacgactagg tgtgcaaatc ataccaccaa ttcctgcttt ttataatcag cctcaaacga
    40201 tccaagatct tgttaatcac catacaatga aacttttgga tcaatttgaa attgtaaatg
    40261 agcattcaaa acgttggcag ggtgattaat atggaaaaag aaacgatagt tgaagtaacc
    40321 aaactaggtt tcacgatcca agctgctgtt aaagtaatcg gtaaagactt attgatcact
    40381 ttaacaggcg gagatacacc acatatcgga acagtcacta cattttctcg tgatacagag
    40441 atgcaaactg tacgtttccc tagccacgat ggtcggttcc ataaagacga tgtcttagcc
    40501 gaaaaaatag ctgtgatcat caaaactgcc gttccgggaa gttgtacgat cacttccggt
    40561 atccatgttg accatatctc caatgcacaa atcaacgcct cttttccgat gtcagaagag
    40621 ttaggttaca agattttaga ttggttggaa aaaatagatt tccatatcaa agaaccagtt
    40681 tattataaag atggtgaaaa accattatga aagaagaggt tgtgaaatca gcgttatgac
    40741 ctgagtatga gagaaaaaaa tggaaaaata gcttctcata ttttgccgtt tttgagctaa
    40801 taccgaggga cagctgattg aacgccgttt atcaagaggt tgtgataaag ccgcttcgtt
    40861 tcgagtagta agaaaaattt tgtaaacatg ggtttccaca tttttgcata aatttgactt
    40921 actaccgaga aactggcttt tgaacaccgt ttatcaagag gttgtgacac gtcttttgtc
    40981 acaacctctt ttatttttgc ttcactgtaa ctgctacaat atcaccgatt tcttttccga
    41041 tgtttttacg aatatctttt cgtagtccaa ggatgtagca gactgaacca tctggatttt
    41101 tcacgcccat attcacgata ctgccttcat acggcacatt gtcaaaagtt gcttggactt
    41161 taactcttcc agcaccgaat tcttttctta tatcatacgg aaaaataata tatgccccgc
    41221 ccttgccgac ttcggaagca tagatttttg attcaaaaga ataaatttcc ctcattttat
    41281 ttctcctatt ttttacatct agtatagcat agcaaaaaaa agaaggaact gtgacaaaag
    41341 tcttgtcgca gttccttttt tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa
    41401 gtcacaatag tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag
    41461 ttaaacggca ggctcacaac ctcttcctta aacggtgttc aaaagcttgc atctacggca
    41521 ctaagcccaa tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt
    41581 gatgcagagc agctttttca caacctccta catggtgttc aaaatattat tcgccttttg
    41641 cttctttcac taattttgcg aatgcttgtt gttggaattc agcagctaag gctttcgtac
    41701 gttgttcttg ctctgctttg ctcatttttg gattttcagt cattgctttt accataccgt
    41761 cttgaacata agcttttgct tcactttcca tcgtactttg ctgtttcttc actaattcag
    41821 ctaacttggc tgcttggtcc gtacttaatt ctacccatgt ctctgggatt ttgaatgtat
    41881 tcactcggct gtcatattcg tgattgttat cagcaatatt gaaccagaat ttatacatgt
    41941 cgtttttgta ttcccaataa gtagttttcg tcactttttc agcatcgccg tctgtctttt
    42001 ctactttatt tgtttcgtta tccgtaccag ttgcttttgg tttttcttgt ttttcatttg
    42061 ttcggtaaag atacactttt tcttttccat tccctaaagc tttatacaaa agcatatctg
    42121 ctccttggga atcagcactg gaaacaattt tttctgtttt tgtttccgtt actttttcca
    42181 ttccaaagtg attggacaaa ttagcgacga tcaaacttag tgaagcgacg aagattagtc
    42241 cgaaaatcaa agataaaaag gtttgccatg tttttttagc gaacacgttc gttaaagcaa
    42301 aagcaagcaa gctgacaatc aaaactccaa taatcatgca ttatcccccg ctttcattag
    42361 aacaggttct tttttcttca ataaaaaggc aataataaag ccaagaatcc caaagatgat
    42421 cgctacgata aacgcggcat ggtaacctga cagtgtggca ttgattgcct gatctttata
    42481 agctaatggc gtattatcca atacagattt gcttgggata tttgcttttg tcacattcgt
    42541 caacacactg atcaaaatag ctgtaccaat cgagcttgcg atttgacgga atgtattatt
    42601 taccgctgtc ccatgactga tcaagttatt tggcaatgca ttcataccag aagtcgttac
    42661 cggcatcatg accatggaga tcccaaacat acggatcgca taaaggatca cgatatataa
    42721 aactggagtc gtttttgtca agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat
    42781 ccctgtgatg gctaaacgtt tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat
    42841 catagccccc atgatcaatg cacccggcaa caacatcaag ccagaatgga atgctgattc
    42901 tccacgaata ttttgaatat acaatggtac gaccatttct gcccctacca tcgccatatt
    42961 tgttacacca gctaaaacag ctgcaatcgt gaatgtagat gatttgaata cacgaagttc
    43021 caagaatgga tgttccaaat gtaattgacg ccatgagaac aaggcgatga aaatcacacc
    43081 aacaactaag aaaccaatta ctttcaagct tccccagccg tcatctccta cactagaaaa
    43141 tccgtaaagt aaactaccga atccaatcgt tgataaaata gcggataaaa tatcaatgct
    43201 aggatttgac aatggaataa cacttttcat taggaagaat gctaaaacca agacaagaac
    43261 aacgattggc agtaccatac caaacaagtc acgccaatgg tagttatcaa tgatataacc
    43321 agataatgta gggcctaaag caggagcaag tccaataacg atccctgaca tccccattgc
    43381 tgatcctctt ttttctggcg ggaaaataga tagcatgata ttttgcaata atggcatcga
    43441 tactcccaca ccgcaagctt ggatcaaacg accgattagt aatgtactaa aattaggtgc
    43501 tgtaaagcaa gtaatcgttc cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct
    43561 tgaactgaat ttattgatca gccatgctgt tattgggatc atgatcccgt tgactagcaa
    43621 gaaaccagtc gttaaccatt gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt
    43681 aggaaaagct gtcgtcaata aggtctgatt caaaacggta caaaatgtac caattaataa
    43741 gacaacaact aaaagtgtcc gattatatgg ttttccgtaa atatctacgg gttggttgtt
    43801 actcatattt ctccttcttt ctgaattttt tctgaaacta ctagaatact ttagtcctaa
    43861 attcgctatt tgtcaaccta cttgacacat aagttaatac ggatataatt aagggaagca
    43921 agaggacaaa aggagtgaca aaaatggttg gggcttccct taaacgtttg ttggaaaatg
    43981 atcaattggt tgttggcatc agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat
    44041 taaggtattg ggaacaaaag ggattcattc gttcgatcga agctgatgcg aattgtccta
    44101 gaaaatatcg tttacctaca gtcataaaag tagaactgat caaaaaatat ttagatgacg
    44161 gatttacttt gtcaaaagct gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg
    44221 tccgtaaagt tttttcaaaa gcaatcaaag atatcgaatt gatcgacgaa cgctatacga
    44281 ttttacaaat cggtaacttc aaagaagaga atgctaagat gtacatcatt catgacaacg
    44341 agacagacga attaacttac aagctccttc ctgtcgatga aaaagctgac tacgaacagt
    44401 tgttaaaaga actctaaacg ccactaatct cctaagtacc aagcttttta ataagtagat
    44461 gctacataaa tacttgaaaa aaggcctgtt tgaacataag aatatccgaa cggtgatctg
    44521 cgtttctttc atatagaaag gaattctcca tcactgttcg gatatttttt tatttttttc
    44581 ggttactttc ttctaataca tctgttagcc aatttgcata ttctgtttcc cgattgatcg
    44641 catgcgctaa aatcaggtag tgtccgtaat tttcatgttt ttctttttca gtaggaaaga
    44701 tcgttttttt tcgctctaat agatgaagtc gtttttcttc atgaaggtca cgctgctgct
    44761 gaataatttc tactaatgcc ggatcattca cgtctttgac gaaatacaat ttcagaacaa
    44821 attcgtctcg attaaccggt agttcattgg tgggtgtatg aatccactct gccaattgtt
    44881 ccttcccttc ttctgttaat tcataaactt tcttttccaa tttttcacct gtgatctcta
    44941 cttgatgctg aatgattcct tgctcttcca tttttgcaag ttcaggataa atctgactat
    45001 gttttgcttg ccaaaattct cctatttcat ttttgaacga ttgattcaat tcatatcctg
    45061 tcatcttctt cttacttaat aaccctaaca aaatatatcg aagtgtattt tttcttgcca
    45121 tcagttttcc ctccttgaca aatctttaaa tccattctat cataaatgag taatcgaaaa
    45181 catgtagttt attacatgtt ttaatttata aggagatgct taaacatgaa agaatttaaa
    45241 gaattggaag attttattgg aactcacttt atctatacgt atgacaatgg ttgggaatat
    45301 gaatggtatg ccaaaaatga ccatactgtt gattaccgta tccatggcgg aatggtcaaa
    45361 ggccgttggg taaaagacca agaagctgat atcgttaaat tgacggacgg cgtattcaaa
    45421 atcacttgga cagagccaac agggacagac gtggcattgg actttatgcc aaatgaaaag
    45481 aaacttcacg gaatgatctt tttccctaaa tgggttgaag aacatccaga aatcactgtt
    45541 actttccaaa acgaacatat cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat
    45601 ccaaaattac ttgttcctga atttgctacg atcacttata tgggtgatgc aggtatagac
    45661 aatgaagatg ttattagcga agaaccatac gatggaatgc cagatgatat ccgtagcgga
    45721 aaatacttcg atgaaaatta taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa
    45781 tcagcgttct gacctgagta ttagagaaag aaacggaaaa atagttcctc atgttttgcc
    45841 gttttttgag ctaataccga gggtcagctg attgaacacc gtttatcaag aggttgtgaa
    45901 atcagcgtta tgctttgagt aataagaacg ctagttagaa aatagctttt catattttct
    45961 aggctagtgg gcttattacc gagaagcagc cgtttgaaca ccgtttatca agaggttgtg
    46021 aaatcagcgt tttgacctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
    46081 gccgtttttt gagctaatac cgagggtcag ctgattgaac accgtttatc aaagattgta
    46141 acaactgcat attcacacag ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa
    46201 aaaattaggc tgggagcatt atttcgttat tctgttcatc aaaaatcagc gttttgattc
    46261 catttcatca caagttcctc ctcatttact gcttcatctt tttgctgtgt ttgaattgta
    46321 aaaccttgtg aaagatagaa atgaatagct gattgatttt tttgatagac tgtcaaagtc
    46381 agtgttttat tggtttcttt tatttttttc attagttgcg taccgattct ttgttgccgg
    46441 tattcccttt tcacaaagat tcctgcgata aactgatcaa tcaccccagc aaaaccaata
    46501 atttcttgat ctatttctgc taagtaaatc actgcatctg gtaaagcttt cctaacagat
    46561 tctacttgat tctcccaata agttgatgga atgaattcat gagaatccaa gttagcatgc
    46621 agccaaatcg atagaacaga ttccagttct ttggcagaca attttgttgt tgtgcggatc
    46681 attttttctc tccttactta ttcattgatt ttaaaataaa aaccattttt ttgaagctct
    46741 tttacaagtt ttcgcccctc aaaaaagtat atcttctctt gttttacttt tctaatacca
    46801 aaagaaacag caactttagg atcatcagct gaaacgattc gataagttcc accaggcaat
    46861 tgatccagct tgactttccc atgatggtcg ctttgtactg aaaacggcac accatttcta
    46921 gtaagtgttt ttttcttatg tttcaaaatg aatgaactac catttaccgg tttccctgac
    46981 gaatcactaa taataaatga aaaatcaagc acttttttac ttaatctata ctgatagatt
    47041 agtcgtccga taaacactaa cagtatgact acagctatta tcgttcctgc aagaataaac
    47101 cattgccgca tggttctttc ctgatttcct tttccttctt ctttttttac cgattcgtta
    47161 taaggaatcc gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata cggcgtacat
    47221 gtaagcaagg taactaagtc ttctccttct gaaatcgtta gaacagacgt gtcatctggc
    47281 agtaccactt gtatattttt aacttgataa gccaatttct cccccaaaac agtcaaaata
    47341 aacgtgtctc cgttttctaa acgatctaat tcggtaaata accgtcgatt aggtaaaccg
    47401 ctgtgagcag agatcactga atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc
    47461 aagacgcctg ctccattttc aaggatccta ccattgagcc gatcataaag tgaaatattt
    47521 aattggatct taggtatatt gatacttccg atcagccatt cctctaattg gctggatgtt
    47581 gtactttcat ttctgttctt ttcattgaaa ggatcagtac caggattgaa tccttggtca
    47641 gttaatcgtt ggtttttctc ctccattttc tttcgctgca cttcaatttc agctgcggtc
    47701 cgtttttgta cttgttcgat acgtttttga tcaataaaac tatttaaaga atccacataa
    47761 aatggatata aagcgatcag taaccctacg acgatacata ttaataaaaa caaacgaaac
    47821 aaaagtcgac gagactgttt ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt
    47881 ttttgttctt tcttttatta tatgggttca ggaaaaaaat agaaaatgaa agccgatgag
    47941 gttgtgaaaa agctgtcctg catcaagtaa taagtacagc ccaaaaaaat agctactcat
    48001 attttttgtt gggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    48061 gaggttgtga aaactgcgtt atgctttgag taataagagc gctagtcaga aaatagcttc
    48121 tcatattttc taggctagtg agcttattac cgagaagcag ctgattgaac accgtttacc
    48181 gagaggtcat gaaaaagcta ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa
    48241 ggagatgcaa caaaacttat gtcgcatcct ctctccttag aactttcact tatgtttttc
    48301 ttgtaaaatt cataaatatt ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg
    48361 atatatgatg aatttatcta aagtttgcaa aggagttcga caattgaaaa aatatggccc
    48421 atttttagca atttttttca ttgctttggc atttctcatg cctcatttat tcacacataa
    48481 actaattttg ggtgctgatg ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca
    48541 aattaaatat ggggatttcc attattttcc ttccctttat ggattccagc aaagtggcag
    48601 gatcgtgaat gccgtttacg gcccattttt cgcttattta caaggattga tcgtgttgct
    48661 tgcaggtagc tggtttcatt atcagattct ttcaaatttc attctttatc ttatttcagg
    48721 attttctatg ctgattttac tgagatatgt caatgttcga aattgggtaa gtgtaccagt
    48781 agccgtttta ttcatgagta cgtattctat tcaatactgg tctatcaacc aaggattcac
    48841 tagctggggg acttgttttt atcctttatg tatgatccct attgctgatt tcgtccttca
    48901 taaaaagttc cctgttatca aagtcgctgt ctcagtggga cttatgacac agatccattt
    48961 actgtcttcg atcatgttag ctttgatgta tatccctttt tatctttatt acttttttaa
    49021 ccagactgaa aaaggaaaag cactgcttca actgattaag tcggtcttgc tttattttgt
    49081 tctgacagca aatatttggg caggattagc tattgtttac agtgggaata agattcttcc
    49141 tccctttgtt aacagagaaa tgagtcaaaa ggcgatagat ctggatggca gttattggct
    49201 ccattaccct aaggcctttc ctatattgat attatcaggt tttttgcttt ttattttctt
    49261 tcaacaaaag aacaccttat tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt
    49321 tctttctact agcttgattc catggacgac attgatcgaa aaaaatgtgc cttttattag
    49381 tttgatccag tttccatttc gatttttcgt gccatttacg ctattgcttc tactttacct
    49441 tgccctttca ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg
    49501 cattcttatt ttttcagcac agacgatcca gaacttatac cagcatctgg aaaagtggga
    49561 aaatgaaaca tttgtcagtc gacatactta tctattcgat acgccggaag aagcaaggaa
    49621 aacattcttc agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga
    49681 ctatctgcca atctataagg agacaaaaga caataaatac gaccggtata acgaagaaat
    49741 actagaaaac gaaacaactt ttataaaaac acatccaggc ggtcttttga caatcaagtg
    49801 gaaaaacact ggggacaaaa ctgttcatat ccctgttatc gtctacgatc ggactgtgct
    49861 acagcaaaat ggaaaaacat tgactgatta tgaagtaaca gatatcggga ccccaatcgt
    49921 gaagcagcaa aaaggaatca acgagctcac gcttcattat caaacgccaa tctatttcta
    49981 tttcattctt tctcttactt tgattggctg gtttaccttg ctatgtttat ttatttatca
    50041 tagatacaag cttttacgtg cttaggcaaa agggataaat acatccgatc aaaaaactct
    50101 ccaaacggta taatcgtttg gagagtttta ttttaatcag tttttttatg ttatatgtta
    50161 tattttttat tcgttcacat ctaattcgat ttccaccccg tagtgatcac tgatgattgg
    50221 actatcgatc ccatcaaaaa tccgttgata ttttttaacc tgcattcctt cagggacgaa
    50281 aataaaatct atacgcaatt tttctgtatt ttgttcccaa ccatctattt tcttttccac
    50341 agtagctgct tttccttttt cattcgcgac aacatacgaa tcttgaagaa gaaggcaact
    50401 ttctcttaca gtataataac cttgagagtc tactggatta ttgaaatcac ctaggaaaaa
    50461 aagtggtacg cgagtttcca aaaagtagtt ttccaagttt ttccactcaa aataaaagcc
    50521 acttttttta tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc
    50581 tatctttgtt ttcccaatca agatcttgcg tgtctgataa tcttctttct tttgaaattg
    50641 ggagacggta aggacctgac tttccaatgt ttttttcgta agcagtgcat ttccttcttc
    50701 atatttatca taaccgatgt ggctcatttc ccaactccaa aaatactctt ttccgtgttc
    50761 ctttaaaagc tgaactaaac ggtaggcaaa attatcttta cggataggta cttgattctt
    50821 taccggacag aacttcatta gcttcgcagg ttctacttct tcagattcca gtaattggtt
    50881 gatttcttgt aaagcaatca cttcataatc ttctaacagg atcttatctg ctaattcttg
    50941 caattttttc tctggttctg gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat
    51001 tggttttctc cttgtcttca tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac
    51061 ctctccagca aatagtgttc tcctgctaac tcctcagtaa taagctcaat ggaccaggag
    51121 ctattttttg tttggctgtt tttatcactt ggtgcagggc agctttttca cgacctctcc
    51181 actaaacagt attcaaactg ctgcttctcg gtaataagcc caaccaaacg aaaaatagga
    51241 ggagctattt ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc
    51301 tccttacatt ttaatggagc cgatcacttt accagctgtc actgtaccta attgcttgat
    51361 cactacttct tctactttat cgtttgtaaa tacgatcatg atcgttggat ctttttcttc
    51421 gttttggatc acatcaagat ccatctcagc taatttcgtt cctgttccta cttcttggcc
    51481 ttctttgaca gcaatctcaa aagctggctg attcatttgg actgtatcga ttcccatatg
    51541 gatcaaaact tcgataccat ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat
    51601 acttgtcact tttccagcga tcggcgcaac aacttctctt gatgttggaa taacagcaaa
    51661 gccgtcaccc atcatttttt gtgagaatac cggatctttt acttcagata aaggaatcac
    51721 ttctccagaa gctgcggaaa ctagttcttg ttctatacct aagtattgct tactacctgc
    51781 ttgtccattt cctgtagaaa ccatttctgg ctctggaatc tctgctcctg attgcagcag
    51841 atcttcgata tctgatttca agacatctgc ttttggtcca taaactgctt ggactccatt
    51901 gtctttgacg atcaatccca tcgcccctgc tcgtttccag gcttcttcag tccctacttt
    51961 ttctcgatca tttacactta cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt
    52021 ttcttttcct cccagcaaat gaatgatctg gaccacttgc tgatctgctg ttcctgttcc
    52081 gctgttagat acaacggttt cttcactgtc attgtcgtaa tttccgttac gtcccggtgt
    52141 agcaaagttg aattttttga tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc
    52201 gatcacacac aacacgaagt tgataagatc tccacctaga cccgctttga tagctaacgg
    52261 tgtccgagtc agcaattcga tattcccaaa tgaatggacc cgcaatggca agatatctgc
    52321 catcgcaaat gcagcccctt ggatcactga ataaatgaca tataatggca cagcagcaaa
    52381 cataaacatg aactccaatg gttctgttac acctgttaga aagacagcca acgctgctga
    52441 aaaatacatt gatttatatc ttgctttctt atcaggatcg acattacgat acatcgcaaa
    52501 agccattccc atcaagatac ctgaagaacc aatcatttgt cctactttga aacgagccgg
    52561 cgtccagtta tgcaacacaa actcatattg cgacatatcc cctgcacctt taagattcac
    52621 taaatcagtg gcccaagcca accataacgg atcttgccca aatacttgtg tccccgcctg
    52681 tgcgcctgat aaaatctcat acgatccgcc aagctgtgta tagttgatag gaatcgtcag
    52741 catatgatgc aatccaaatg gcaatagtaa acgttctaat gttccaaata aaaacggtgc
    52801 tagaatcggt gcgctttctt gtgattctgc aatccataaa ccaaaattat tgattccagc
    52861 ctgtacgttt ggccacacca cagcaagaat caatgccacg attgttgacc ataagataac
    52921 aacaaacgga acaaagcgct ttccattgaa aaatgacaaa gcatccggca attttctata
    52981 attatagtac ttattataag ccattgcccc gacaaatcca gcgatgatcc ctacgaatac
    53041 tcccatattc aaagctggtg cttccaatac acttgtaaaa aatcctttaa ccatgatctt
    53101 cgtaccgaac agagtgtggg taaatgcatc ttcattcgca agcatgtcac ttgtgacacc
    53161 aaagatagcc ccagtgatcc gattgattaa aataaatgag atcccagctg caaatgctcc
    53221 gcctgctctt tcttttgccc aacttcctcc tattgctaaa gcaaataaaa tatgaagatt
    53281 accaataatt gcccaaccaa tattttcaat tactccgccc gttgtgacaa gcggcgtcca
    53341 atcaggattg atcaagggaa ttgatttccc tatactgatc atcaacccag ctgccggcat
    53401 aactgctacc acaaccatta aagcctttcc aaatttttgc caaaactcaa aactaaatat
    53461 ctttttcatt ccgtttagct cctttacgca atcggtttcg ttaaacgtat tatagcgtgc
    53521 gttttctttt ttgtaaatgc tttatttttt atttttctgt ctataccatt ctataaaacc
    53581 tataatacgt gtgttccctt ctataatttt ctcctaaaaa tttatttttt taagcttttt
    53641 tctatgaaat attttctttt gtctattgac ttgtgcaatc ggttgcgttt attatgaacg
    53701 tataaaacag ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga
    53761 tccatggaaa atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt
    53821 aacaagtatt ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg
    53881 ggaccaccat caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg
    53941 ctggtggaaa aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat
    54001 cgcgatagat atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga
    54061 cttttactgg gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac
    54121 ttctactaat aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc
    54181 agcttatatc tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc
    54241 aaaattggat ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga
    54301 gattgatcgt ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa
    54361 ctttgggatt gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc
    54421 agtcatccct gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt
    54481 atctcaaggt gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat
    54541 tccagaagcg cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta
    54601 tatccaagca aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt
    54661 ggtcatcgaa ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt
    54721 ctctacttac tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga
    54781 aaaatacggc ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc
    54841 tttagccaaa cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca
    54901 agcaatccac aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt
    54961 cactggtgtc gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg
    55021 cgcgattgcc tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa
    55081 aaatgaagga ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt
    55141 ttcaaaacga gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa
    55201 caatatcaat aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg
    55261 agaaaattat ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc
    55321 caaatggaaa gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt
    55381 tgtccagcat gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag
    55441 cgatcttcct ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa
    55501 gcaggcagat gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa
    55561 gcaaaaaaac tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag
    55621 tatccattcg attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca
    55681 gcgaacagct cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat
    55741 cacttccatg actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc
    55801 ccatgatcag ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca
    55861 tatcaattat cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga
    55921 gttattagaa ggttctcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga
    55981 tcgtctagaa ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt
    56041 tagacggtgt cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc
    56101 aggaaatcgg cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag
    56161 aagactcact ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag
    56221 aatttgcaga attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg
    56281 aaccaaaaga tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa
    56341 tcaagattgc tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc
    56401 ttacacctta ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc
    56461 cagatatctt cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga
    56521 tcgaagacgc gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg
    56581 tgggacgtgc tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta
    56641 cgatcgatta cttgaaagaa aagtggcatg accatggata atccagagat ctccgtcgta
    56701 ccagttccag agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt
    56761 cttctcaatt acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca
    56821 gaaaatgttt tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt
    56881 ggagcaactg tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga
    56941 ttggagaaaa actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa
    57001 ttttgggaag tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa
    57061 gatgaatttt ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa
    57121 aacgaattag agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc
    57181 aaccatgcct acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta
    57241 acggtctttt tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg
    57301 acaaaaaaag aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt
    57361 gcatgttatc cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa
    57421 acttccttac aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat
    57481 aaaagtatgg tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa
    57541 cagatgcatt caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat
    57601 ttccccaaat ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt
    57661 tatcggttct ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa
    57721 gatgtggcga aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat
    57781 cctagtattt ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat
    57841 gttcctaaca tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc
    57901 ttaccccctc ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa
    57961 gcaatcaacg aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat
    58021 tttgaattgc tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt
    58081 attctagtgt attcagatag tcaagatact gtcattgatt atttatacca aaaccacatc
    58141 cctttcactt tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat
    58201 gataaccagc ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat
    58261 atcctgtttg tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat
    58321 caaaaagcaa tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg
    58381 gaggattatc tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc
    58441 attgacgata tcttcgccct tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt
    58501 ccagagacac tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct
    58561 taccttacaa gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg
    58621 cgcgaattga tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg
    58681 atcaaaagag agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat
    58741 acgctgacca tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac
    58801 attgtttatc tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc
    58861 taaacacgta taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca
    58921 aaaatttaaa aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt
    58981 cccagcctct tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc
    59041 ctaatattgt aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg
    59101 cgctaacttt catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat
    59161 tgacggtcgc acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt
    59221 catcaaaaga acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa
    59281 cgatcagatt gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt
    59341 ccatcttttc ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc
    59401 cacctaatgc caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc
    59461 ctcgagaaag cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca
    59521 agatgtctgc tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag
    59581 cagctgtcga tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa
    59641 ataaaataac tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt
    59701 ggatcatttc attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat
    59761 aaaaaatcaa cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt
    59821 cgaatagatt aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc
    59881 agttattccc taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc
    59941 caagtaaatg accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac
    60001 tgccgacagt cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca
    60061 cagaaacaag tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg
    60121 aagcaactaa gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc
    60181 ctttgatgat cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca
    60241 acaaacacat gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca
    60301 gtactcctag gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat
    60361 ttttattcat cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc
    60421 acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt
    60481 atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga
    60541 tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca
    60601 atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc
    60661 tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg
    60721 gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa
    60781 taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat
    60841 aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc
    60901 cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt
    60961 ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa
    61021 ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa
    61081 tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta
    61141 caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt
    61201 ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc
    61261 caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag
    61321 ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga
    61381 aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata
    61441 caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc
    61501 agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac
    61561 caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca
    61621 tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta
    61681 cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg
    61741 atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga
    61801 ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa
    61861 aggtcccaag acccatttta cttacttcag atttactcac cgttgacact ctccttgatt
    61921 ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg
    61981 aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag acaacctcaa
    62041 aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga
    62101 aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc
    62161 caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc
    62221 agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa
    62281 tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa
    62341 ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc
    62401 attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt
    62461 attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa
    62521 atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc
    62581 attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata
    62641 tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc
    62701 tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc
    62761 aaatacatat gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat
    62821 accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc
    62881 ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt
    62941 aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc
    63001 ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa
    63061 gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat
    63121 tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc
    63181 tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac
    63241 tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga
    63301 atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg
    63361 tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat
    63421 ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt
    63481 cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa
    63541 gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt
    63601 tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc
    63661 aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt
    63721 ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg
    63781 catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact
    63841 agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg
    63901 ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc
    63961 agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat
    64021 agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt
    64081 ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat
    64141 ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact
    64201 ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt
    64261 ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt
    64321 actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat
    64381 tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata
    64441 gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca
    64501 agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat
    64561 ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt
    64621 attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc
    64681 aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg
    64741 atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct
    64801 tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg
    64861 gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt
    64921 aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa tccagctgtg
    64981 atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga
    65041 tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga gattcctact
    65101 tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg
    65161 tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat
    65221 gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta
    65281 tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg
    65341 atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt
    65401 ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg
    65461 ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt
    65521 tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc
    65581 gtgtagtttt tttatgaaag caggaacctg ctagcatatg taattaatta tttcttcagg
    65641 ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt
    65701 accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg
    65761 tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc
    65821 agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct
    65881 tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg
    65941 atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt
    66001 attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga
    66061 agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa
    66121 aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca
    66181 cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta
    66241 caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag
    66301 atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc
    66361 aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc
    66421 ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct
    66481 ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg
    66541 cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac
    66601 tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa gacgaacaag
    66661 gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac
    66721 aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg
    66781 tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg
    66841 actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc
    66901 gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat
    66961 caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag
    67021 tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga
    67081 atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag
    67141 ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg
    67201 actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg
    67261 atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct
    67321 atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa
    67381 atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat
    67441 ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt
    67501 tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc
    67561 attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca
    67621 ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt
    67681 tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca
    67741 tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa
    67801 attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt
    67861 cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa
    67921 atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg
    67981 ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt
    68041 ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct
    68101 tttcaaaacc gttcatttgt agagcctccc gtaatt
//
LOCUS       DAZKOU010000004        62086 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000004 DAZKOU010000000
VERSION     DAZKOU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.004"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(240..725)
                     /locus_tag="ACG827_000200"
     CDS             complement(240..725)
                     /locus_tag="ACG827_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0482375.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    815..1160
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1205..1846
                     /locus_tag="ACG827_000201"
     CDS             1205..1846
                     /locus_tag="ACG827_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0482376.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1865..2500
                     /locus_tag="ACG827_000202"
     CDS             1865..2500
                     /locus_tag="ACG827_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482377.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2518..3333
                     /locus_tag="ACG827_000203"
     CDS             2518..3333
                     /locus_tag="ACG827_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0482378.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3652..5088
                     /locus_tag="ACG827_000204"
     CDS             3652..5088
                     /locus_tag="ACG827_000204"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0482379.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5277..7724
                     /locus_tag="ACG827_000205"
     CDS             5277..7724
                     /locus_tag="ACG827_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0482380.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7954..9021
                     /gene="rlmN"
                     /locus_tag="ACG827_000206"
     CDS             7954..9021
                     /gene="rlmN"
                     /locus_tag="ACG827_000206"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0482381.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9011..9217
                     /locus_tag="ACG827_000207"
     CDS             9011..9217
                     /locus_tag="ACG827_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482382.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9214..10317)
                     /locus_tag="ACG827_000208"
     CDS             complement(9214..10317)
                     /locus_tag="ACG827_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0482383.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10870..11418
                     /gene="aac(6')"
                     /locus_tag="ACG827_000209"
     CDS             10870..11418
                     /gene="aac(6')"
                     /locus_tag="ACG827_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0482384.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(11666..11785)
                     /locus_tag="ACG827_000210"
     CDS             complement(11666..11785)
                     /locus_tag="ACG827_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0482385.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            11964..13625
                     /locus_tag="ACG827_000211"
     CDS             11964..13625
                     /locus_tag="ACG827_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0482386.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13695..14042)
                     /locus_tag="ACG827_000212"
     CDS             complement(13695..14042)
                     /locus_tag="ACG827_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0482387.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            14126..15067
                     /gene="opp3b"
                     /locus_tag="ACG827_000213"
     CDS             14126..15067
                     /gene="opp3b"
                     /locus_tag="ACG827_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0482388.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            15068..16111
                     /locus_tag="ACG827_000214"
     CDS             15068..16111
                     /locus_tag="ACG827_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0482389.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16117..17187
                     /locus_tag="ACG827_000215"
     CDS             16117..17187
                     /locus_tag="ACG827_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482390.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17177..18130
                     /locus_tag="ACG827_000216"
     CDS             17177..18130
                     /locus_tag="ACG827_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482391.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18142..19152
                     /locus_tag="ACG827_000217"
     CDS             18142..19152
                     /locus_tag="ACG827_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0482392.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19437..20258)
                     /locus_tag="ACG827_000218"
     CDS             complement(19437..20258)
                     /locus_tag="ACG827_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0482393.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20355..20633)
                     /locus_tag="ACG827_000219"
     CDS             complement(20355..20633)
                     /locus_tag="ACG827_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482394.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20901..22910
                     /gene="metG"
                     /locus_tag="ACG827_000220"
     CDS             20901..22910
                     /gene="metG"
                     /locus_tag="ACG827_000220"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0482395.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23389..23976)
                     /locus_tag="ACG827_000221"
     CDS             complement(23389..23976)
                     /locus_tag="ACG827_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482396.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            24151..25473
                     /locus_tag="ACG827_000222"
     CDS             24151..25473
                     /locus_tag="ACG827_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0482397.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25628..26404
                     /locus_tag="ACG827_000223"
     CDS             25628..26404
                     /locus_tag="ACG827_000223"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0482398.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26397..26966
                     /gene="rnmV"
                     /locus_tag="ACG827_000224"
     CDS             26397..26966
                     /gene="rnmV"
                     /locus_tag="ACG827_000224"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0482399.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26959..27843
                     /gene="rsmA"
                     /locus_tag="ACG827_000225"
     CDS             26959..27843
                     /gene="rsmA"
                     /locus_tag="ACG827_000225"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0482400.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28294..28923
                     /locus_tag="ACG827_000226"
     CDS             28294..28923
                     /locus_tag="ACG827_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482401.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(29047..30156)
                     /locus_tag="ACG827_000227"
     CDS             complement(29047..30156)
                     /locus_tag="ACG827_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482402.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            30365..30787
                     /gene="mgsA"
                     /locus_tag="ACG827_000228"
     CDS             30365..30787
                     /gene="mgsA"
                     /locus_tag="ACG827_000228"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0482403.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      30974..31072
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            31110..31646
                     /locus_tag="ACG827_000229"
     CDS             31110..31646
                     /locus_tag="ACG827_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0482404.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(31686..33449)
                     /locus_tag="ACG827_000230"
     CDS             complement(31686..33449)
                     /locus_tag="ACG827_000230"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482405.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(33452..35167)
                     /locus_tag="ACG827_000231"
     CDS             complement(33452..35167)
                     /locus_tag="ACG827_000231"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482406.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(35946..36170)
                     /locus_tag="ACG827_000232"
     CDS             complement(35946..36170)
                     /locus_tag="ACG827_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482407.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            36361..39138
                     /locus_tag="ACG827_000233"
     CDS             36361..39138
                     /locus_tag="ACG827_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0482408.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            39219..39638
                     /locus_tag="ACG827_000234"
     CDS             39219..39638
                     /locus_tag="ACG827_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0482409.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            39650..40120
                     /locus_tag="ACG827_000235"
     CDS             39650..40120
                     /locus_tag="ACG827_000235"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0482410.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            40137..40916
                     /locus_tag="ACG827_000236"
     CDS             40137..40916
                     /locus_tag="ACG827_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0482411.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            40903..41724
                     /locus_tag="ACG827_000237"
     CDS             40903..41724
                     /locus_tag="ACG827_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0482412.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            41741..42790
                     /locus_tag="ACG827_000238"
     CDS             41741..42790
                     /locus_tag="ACG827_000238"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0482413.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            42803..43807
                     /locus_tag="ACG827_000239"
     CDS             42803..43807
                     /locus_tag="ACG827_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0482414.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            44440..45459
                     /locus_tag="ACG827_000240"
     CDS             44440..45459
                     /locus_tag="ACG827_000240"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0482415.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            45502..46383
                     /gene="galU"
                     /locus_tag="ACG827_000241"
     CDS             45502..46383
                     /gene="galU"
                     /locus_tag="ACG827_000241"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0482416.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            46683..47060
                     /locus_tag="ACG827_000242"
     CDS             46683..47060
                     /locus_tag="ACG827_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482417.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            47666..48280
                     /locus_tag="ACG827_000243"
     CDS             47666..48280
                     /locus_tag="ACG827_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0482418.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            49053..50792
                     /gene="yfcC"
                     /locus_tag="ACG827_000244"
     CDS             49053..50792
                     /gene="yfcC"
                     /locus_tag="ACG827_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0482419.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            50949..52133
                     /gene="iadA"
                     /locus_tag="ACG827_000245"
     CDS             50949..52133
                     /gene="iadA"
                     /locus_tag="ACG827_000245"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0482420.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            52244..52447
                     /locus_tag="ACG827_000246"
     CDS             52244..52447
                     /locus_tag="ACG827_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0482421.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(52548..53210)
                     /locus_tag="ACG827_000247"
     CDS             complement(52548..53210)
                     /locus_tag="ACG827_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482422.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(53223..54725)
                     /locus_tag="ACG827_000248"
     CDS             complement(53223..54725)
                     /locus_tag="ACG827_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0482423.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            55273..56952
                     /locus_tag="ACG827_000249"
     CDS             55273..56952
                     /locus_tag="ACG827_000249"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0482424.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            57079..57558
                     /locus_tag="ACG827_000250"
     CDS             57079..57558
                     /locus_tag="ACG827_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0482425.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            57777..58457
                     /locus_tag="ACG827_000251"
     CDS             57777..58457
                     /locus_tag="ACG827_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0482426.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            58465..59796
                     /locus_tag="ACG827_000252"
     CDS             58465..59796
                     /locus_tag="ACG827_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0482427.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            59811..60773
                     /locus_tag="ACG827_000253"
     CDS             59811..60773
                     /locus_tag="ACG827_000253"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0482428.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            60884..61222
                     /locus_tag="ACG827_000254"
     CDS             60884..61222
                     /locus_tag="ACG827_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0482429.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            61301..62008
                     /locus_tag="ACG827_000255"
     CDS             61301..62008
                     /locus_tag="ACG827_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0482430.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT        20300 a        10790 c        12996 g        18000 t
ORIGIN      
        1 gaaatcaaaa gaataaaatt ccgatttata atcgaaaaaa gaagcatttt tatatatgct
       61 tcttttttcg tagaatcaat aaatatggat ttggctaact atggaagaat actcgtatta
      121 gttttcagcg ttcatactag ttgttacatt gtttgattgg ccaaaagaat ttctgatata
      181 ttttgtgcta aataaagaaa aagacgcttg aattgggaaa taagaaaact actctatcat
      241 tacaaatttt cataaactgg cagatgttct aagtaaacaa gtatcttttc aaagtcggaa
      301 aaatccataa tcaacgtagt ggtattgata tttggatgaa agcctacggt atcttgacga
      361 tcaatagcag catcgatcac gacttggatt tttttctctt tgtcgtgcat caatgctaat
      421 ggagtcactg tacctgctgg aacgcctagg aggtcaggaa gctgttcttc agaaacaaaa
      481 gataagcgct tttcttttaa ttgttcagct agttgtttta aatcggcgtg cttttcatca
      541 ggcaagatga caagatagat ttgttttcct tttcttgatt tcaataatag attttttact
      601 tgcggaccag gtagggaaaa agggacattt ttgactgacg taatcgcagg atgatcgact
      661 ttttgaaaag agatacccag tttttttagt agttcataag cttcttgttc ggttgcacga
      721 ctcatcgcgc atcaccctcc tttttttatt agtgtatcat aaaaaagagt tgacaaatga
      781 aaaataatca tgtaaattac acgtaaagtt aaaacaaatg acatcgaaaa gaagagtagc
      841 cgttaggtgt ttttagagag tctctggttg gtggaaagag gcagcagtta accgtgaaac
      901 acatcttgga gtcgattttc tgaagcgtat agtaggaaaa tccggggagc ccgttacagc
      961 tgcggtcgtt gctagatgac cagataaggc tggcttttcc gtgaggagtc ggtaaaaaaa
     1021 ggtggaacca cgtgattaac gtcctttgat cttcggatca aaggactttt tgttttcatc
     1081 tgaacggcaa cgactgtgtg aggttttttc tgcaaggaaa aaataaacag aggtggaacc
     1141 acgtataata cgtcctcttg gcatttgcca agaggatttt tttattttaa aggaggaaaa
     1201 gaagatggag tatttgattg aagttatgcc gcaattatta gtaggagcac tcacaacatt
     1261 gaaattattt ttatttacct tgattggctc attaccatta gggattgttt tagcattagg
     1321 attgtctttg cattttaagc cgctacgcta cttgcttcaa acatatgtat gggttatgcg
     1381 gggaacaccg ctgttgttgc aactgatttt tattttttat gggttgccga cgattgggat
     1441 cgtttttgat cgtttcgaag cagcactcat tgcctttatc ttaaactatg cagcttattt
     1501 tgcagagatc tttcgtggag ggatacaatc gattcctcaa ggtcaatatg aagcagcgca
     1561 agttttgcaa ttaagtccct ttcaaacggt gacgaaaatc gtcatcccac aagtgacaaa
     1621 gattgtttta ccttctgtag ggaatgaagt tatcaacttg gtcaaagatt cttccttgat
     1681 ctatatatta ggtttaggtg acgtaatgcg tgctgggaaa atcgcgatgg aacgcgacgc
     1741 gactttactg cctttgctag gcgtggcaat catctatctt acattgactg gtattctaac
     1801 aatcataatg aaacaagttg aaggaaaatt aaattattac cgctaactga taggaggaag
     1861 aaatatgatt gaattgaaga aagtgaacaa agcatttggt gaaaaaaaaa taatcaaaga
     1921 tttgaatcta atcattccag atgggaaaac attagctatc gtaggtcctt ccggtggcgg
     1981 taaaaccacg cttttacgta tcttggctgg cttagaaagt tctgacagcg gagaatttta
     2041 tttagacggt gtaccgttca acccattcga gcttaaaatg aaagaacaag ttgtaggagt
     2101 cgtattccaa gacttccagc tatttccaca tctgaatatt tttgaaaaca ttaccttagc
     2161 accaaaaatg gtattgaaac agacaaaaga agaatacacc aaaaaagcag aagaattagc
     2221 ggcttcatta gggattagtg atttattgaa tatctatcct tatcagcttt ctggcggaca
     2281 aaaacagcgg gtagctattg ccagagcatt ggctatgaat ccacgggtat tggcttatga
     2341 tgaaccaaca agtgctttag atcctgaatt acgccagcaa gtcgagcgtt tgatcctatc
     2401 attgaaagaa gatggggtaa cccaaattat tgtcacccat gatttagtat ttgcagaaaa
     2461 tgtcggtgat caattactaa aagtttcccc aattcattaa gaaaggagaa gatagagatg
     2521 aaaaaaacca gttttctatt cgctttgatc gcaggtcttc ttctttttgc aggctgttcg
     2581 aacaaaaaaa cttcagcaga taattgggcg actatcaacg aagagaaaaa aatcgttata
     2641 ggtttagacg atacatttgt tcctatggga tttagagata aagacgggaa gctcacagga
     2701 tttgatattg atttagccaa agcagtcttt gaacaatatg ggattacggt agactttcag
     2761 cccattgact ggtcaatgaa ggaatttgaa ctgaacaatg gaacgatcga tttgatatgg
     2821 aacggctatt ccaaaacgcc ggcaagagaa aagaaagtgc aatttacaaa accttatatg
     2881 gaaaacgatc aagtgctgat cacacctaaa gaatcaggaa tcacaaactt tggacaaatg
     2941 aataataaga gattaggtgc gcaaaatggg tcatctggtt atgatgtatt tacgaaacaa
     3001 ccagaagtat taaaagagat agtcaaaaac caagatgctg ttttgtatga tagttttaat
     3061 gaagcattga tcgatctgaa aagcagacgg gtcgatggtc tgctgatgga taaagtatac
     3121 gcagactatt atttgaaaca aagaaacgaa ctagaagact tcaacataac aaaaggttct
     3181 tatgagagtg aggattttgc agttggtgca cgcaagtcag atagtacatt agtgaaaaaa
     3241 atcaatgaag ccctagatca actagagaaa acaggtgaat tccaaaaaat atccgataaa
     3301 tggtttggcg aagatgttac gccgagaaat taacaaatag gggactgtga caaaagacgt
     3361 gtcacagtcc ctttttccga ataaacagtg ttctcctgcc aactccttgt gactcggagc
     3421 tgaatggcag gctcacaacc tcttggtaaa cggtgttcaa aagccagttt ttcggtatta
     3481 agccaaattt atgcaaaaat gtagaaagcc attttcacaa aatttttctt actactcgaa
     3541 actaagcgac tttttcacaa cctcctttac ataattttat tttgttgtgg aaggggtttc
     3601 gtgttattct aaaagaggaa cgattgaagg ttataggaaa ggaagagatg aatggaacac
     3661 aaagaactta cctcatttcc aaaagatttt ctgtggggct ctgcttctgc agcgtatcaa
     3721 gtagaaggag cttggcaaga agatgggaaa ggagaatcag tctgggatcg atttgtacgg
     3781 atacctggaa agacatttaa aggaacgaac ggtgatttgg cagtagacca ctatcatcgt
     3841 tataaagaag atattgcttt aatgaaagaa caaggtctga aagcttaccg cttctctgta
     3901 gcttggacac ggatttttcc taatggacga ggagaagtca atcaagctgg gctcgctttt
     3961 tatgagagat tgatcgacga attgattgaa aacgatattg aacctatgtt gactttatat
     4021 cattgggatc ttccacaagc attgcaagat gaatataatg gttgggaatc acgtcagatc
     4081 attgaagatt tcactcatta tgcagaaaca ttatttgagg cattccgcgg gaaagtccat
     4141 tactggatta gtttgaatga acaaaatatc ttcacttcat taggttattt gttagcagca
     4201 catccgccag gtgtgacaga cccgaaacga atgtatgaag ttaaccatat tgctaattta
     4261 gcaaatgcat ctgtcatcaa taaatttcac gagttagaaa tgcctggaaa aatcggacca
     4321 agctttgcgt atacgccaaa ttatccaatt gacagtaatc ctgaaaatgt tctggctgct
     4381 gaaaatgcag aagacctgat ggccaattat tggatggatg tctatatgtg gggaaaatat
     4441 ccgattgctg ccatgaggtt cttagaagaa aaaggatggg caccaacgat tgaagctggc
     4501 gatgcagaat tgctagaatc tgctaaacca gattttcttg ggatcaatta ctatcaaacg
     4561 gcgacgaatg cattcaatcc attaaacgga gttggtgctg gaaaaatgaa tacgacagga
     4621 aaaaaaggtt catcagaaga aactggagtt cctggaatgt ataaaaaagt tgagaatcca
     4681 tttgtcgaac gaacaaattg ggactgggag attgacccag aaggattaag gatcggactg
     4741 cgtcgtatca caagccgtta ccgcatgccg gtactgatta cggaaaatgg cttaggagag
     4801 tatgacaagt tgacagaaga taaacaaatc catgacgact atcgaatcaa ttacttacaa
     4861 agccacgtca aagccatcaa agaagcaatt tcggatggag cagaagtcct aggctattgc
     4921 acctggtctt atacagattt gcttagctgg ctgaacggtt atcaaaagcg atatggattt
     4981 gtatatgttg atcaagacga aacacaagaa ggatctttgg aacgatataa gaaagacagt
     5041 tattactggt accaaaaact gattgaagaa aatggagaga acctttagtt cacatactta
     5101 aaacaagaag gtacggctgg atatggccgt gccttttctt gataaaatca tttagaaatt
     5161 aagaaaaact gtaaaataag ctgaaaaatg aaagaaagcg tcagttcttt caaacaaaac
     5221 tttagttccc ttactttttt ttgtataatg aaacttgatg aaatgaggga taataaatgg
     5281 cacaaaagaa acggcctact aaaaaaagga aaaagacgaa aaaaacgaaa aagcaaaaac
     5341 agcagcagga acaaatgctg acgattggtg ttgggtttgc cttgatttta tttgctgtct
     5401 tcggcttttt aaaattaggt tttttaggaa tattgattgc aaacggcttt cggatcattg
     5461 caggaaatac ttaccaaatc ctctgtcttt tattagccgt attaggattt tggattgtca
     5521 ttaagaatac agagttttca attggtaaaa accgacgctg gtttggcggc atactctttt
     5581 atttcggtat cttattattg ttacatgccc atttatttgg caaactccat actggggaac
     5641 caaatatcat ggggacgaca tgggatttgt tagccagtga tataaaacaa agtcaagtag
     5701 acaataacgt tggtggtggg atgattggag caatccttta tcattttact tacttcttga
     5761 ttgcacagcc tggaagttat ttagcagcag tgctattact ggcaggagga gcattcctgt
     5821 tcagtaatct ggaaggctat cagctgttaa atggtatcca atcaattgga gaacgagtac
     5881 aggaattatt ggaaggtgat cctgccaagc aagctcgaaa gcaagcggca aaggaagaga
     5941 gaatgaaaca gcgcgcagaa gcgaaagaag caaggcgctt agctgcaaaa gaagctgcag
     6001 aaaaagaagc tgcagaatat gagaagaaaa aagcggtgag ccagcaaagg gaaaatactg
     6061 ctgatgaatg gcaggaacca gaaaatacag agccggagca gctaagtttt gttccaatcg
     6121 acagttttca agaaaatatc catcctgcga atctagagaa accagtacca gatacaccaa
     6181 aacagaccaa tacagcagaa ggtttttcag atgagttgcc agaggatgac ggtacctcat
     6241 tagaatttga gattgaagca gaacaggaaa atcaagacta tgaattaccg acagttgatt
     6301 tgcttgattc tataccaact gtggatcaaa gtgatgagta taaaaaaatt gaaaaaaata
     6361 tcggcgtatt agaacaaact ttccaaagtt tcggtgtcga tgccaaggtc gtcaaagcca
     6421 gcttaggacc atccgtcaca aaattcgaag tccagccggc tgtaggggta aaagtaagta
     6481 aaattgtgaa cttaacagat gatatcgcgc ttgctttagc ggcgaaagat gtccggatgg
     6541 aagcaccgat tccaggtaaa tcactgattg gtattgaagt accaaatgga aagatcagta
     6601 tggtttcctt tagagaaatc atcgaagcac aaccaaacca cccagataaa ttactagaag
     6661 tacctttagg acgtgatgtt tctggacggg tacaaacggc tgatctttcg aagatgcctc
     6721 acttactcgt ggctggttcg actggtagtg ggaagtcagt agcaattaat gggattatca
     6781 caagtatatt aatgcgtgct aagccccatg aagtaaaact gatgatgatc gatccgaaaa
     6841 tggttgaatt gaatatgtac aacggcatac ctcatctgtt gacgccggtc gtgacgaacc
     6901 ctagaaaagc agcgcaagcc ttacaaaaag tagtacaaga aatggaagaa cgatatgaaa
     6961 aattcgcggc aaccggagta agaaatattt ctggttacaa tgaatttgtt caacagaaaa
     7021 acttggaaaa cggaacgaaa catccaacct taccattcat cgtagtgatc gtagatgaat
     7081 tagctgattt gatgatggta gccagcaatg aagttgaaga tgcgattatt cgtttagcgc
     7141 aaatggctcg ggcagcgggg attcatatga tcctagctac tcaacgtcca agtgtggatg
     7201 ttatcacagg aatcatcaaa gcaaatgtac cttccagaat ggcatttgcg gtatcaagcg
     7261 gtacagattc tcggacgatc attgattcta atggggcaga aaagctctta gggcgaggag
     7321 atatgctatt cttgccaatg ggtgagaata aaccgatccg tgtgcaagga gcattcattt
     7381 ctgaccacga agtagaacgg gtcgttcaat ttgttacaga tcagcaagaa gctcattatg
     7441 aagagaaaat gatgccgaca gatgaagttg aaacgacagg tgctccagaa cagccacaag
     7501 atgagctgtt tgaagaagca aaagcacttg tcgttgaaat gcaaacggca agtatttccc
     7561 ttctccaaag aagatttcgg atcggttaca accgagcagc acgtttagta gatgaactgg
     7621 aagctcatgg agttgttggc ccgtcagaag gaagcaagcc tcggaaagta ttcattgagc
     7681 aggaagaaac atcagaaacg ttgccggacg ataatgacat gtaaaacttg tgtgatgaag
     7741 ggttgaaaaa cgaataacca ataaaaagac aaggcatgat gataccaaaa gaaaaaatgg
     7801 tttttttcca tcttttctaa tacggtatga tgtcttgtct ttttagtttt tgtgagatta
     7861 aagttctatt cgatttattt gtgaagtatt gcattaagga aatgaatcat gtatagtatg
     7921 tagcgaaact ttgagaaaaa gagggaaaat actttggaaa aacagtccat ttatggcttg
     7981 acgaatgaag aattaatcaa ttggtttata gaaaacggcg agaaaaaatt ccgagctgcc
     8041 caagtttggg aatggttgta tcaaaaacgt gtaagtaatt tcacagaaat gacgaatcta
     8101 tcgaaacaat tgatcgaaaa attatcggag cattttatca tcaatcctct aaaacagatg
     8161 gtcgtccaag aagcttcgga tggtacagta aaatatttgt ttgaactccc tgataaaaac
     8221 atgattgaga ctgtactgat gagacaagaa tatggcttgt cagtttgtgt gacgacacaa
     8281 gtaggatgta atatcggctg tacgttttgt gcaagcgggc tgctcaaaaa gaaccgtgat
     8341 ctgactgctg gagaaattgt tgcacagatc atgatggtcc aacattattt tgatgagcgc
     8401 aatcttggcg aacgcgtttc acatgtcgtt gtaatgggga tcggtgagcc atttgataat
     8461 tacgacaatg taatggactt tttgcatatc atcaatgatg ccaaaggatt ggcaattggt
     8521 gctcggcaca ttacagtttc cactagtgga ttagcacaca aaatcaagga attcgctaat
     8581 aacggcttgc aagtcaatct ggctatttcc ttgcacgcgc cgaataatga ggtgcgaaca
     8641 tcgatcatgc ggatcaaccg aagtttccca attgagaaat tgatggaagc tgtggatgag
     8701 tatttagaga aaacaaaccg tcgaatcacg tttgaatata tcatgttgaa tcaagtaaat
     8761 gatagaccag agcatgcgca acaattagca gatctattaa aagataagaa aaaattagca
     8821 tatgtcaact tgattccata taatccagtc agtgaacatg atcaatattc tcgcagtcca
     8881 aaagctgatg tattgagatt ttatgatgta ttgaagaaaa acggcgtaaa ctgtgtgata
     8941 cgtaaagaac atggtacaga tattgatgca gcttgcggac aattgcgaag caagcaaatg
     9001 aaaaaaacag ttgccaaata atcgtatcct actcgttttt tcaattacca taaaaggagc
     9061 agttttctta attaaggaac tgttcaactt aaataagaga aaagattcta tagcgaaagc
     9121 tataaagctt tttttgacaa gaaaaaccct catcaagcaa agaaatgtcc tcaatttgat
     9181 gacagcctgc ttgatggggg ttttagcaat tatttaactg attttcttat atccttgctg
     9241 gctttcttgt gtagcatgat aagtttctac gtcaagaatg gcctgtttaa atttttctcg
     9301 ggtgatttca taaggcacat gtttaatatc atttgttgcc atggctttat ctaagattgc
     9361 ttctaaatcg gtcgtgacat tgagattcat gtcggttaac gatactggaa gttggttctt
     9421 tttcataaac acatagattt tatcaaacaa atcgaactgc tgatccatca tcaataaggt
     9481 cagtacacca taactgacag atgctccgtg aaggtatccc tccatttttt ctagaacagt
     9541 cgtaccgtaa tagaatgagt gtgccatagc agaattatag ttattgttaa ctaaaacaga
     9601 aaccaatccg gtagtaacaa tgatatccga aacgacttgt tcaattgcat atgagggacg
     9661 atcgtcggca ctatctttca atgcttgcgt accataagtc aagagacgat caatgcacat
     9721 ttttccgatt tggattccta atctatccgt gtagcctaga tcatcgccac gagcagaaaa
     9781 agtagattcg acttctttgg ataaagcatc gccaatacct gcccataagt atttatcagg
     9841 tgcttcggca ataattgctg tatcaatgaa ggtgtgtaca ggtggttttg aaggaaagaa
     9901 gagtccgtcc attacgcctt cttcgttata gatgacacat accgcagtga cgggcgaaca
     9961 gttcgaacca atagtgggaa aggtgaagac tggtttagct agtttttcac caatgacttt
    10021 tacagtatcg caagcacggc caccaccgac tgcaaacagg acgtctgctt tttggacact
    10081 ttcatgctgg atcaactgtt caatatttcc ataagttgaa ttacccccgt accagagact
    10141 ggcgacaacc tctatttcct gtgtcaaagc tacttttatt ttgttttctg cctttgaaag
    10201 tgcagtcttc cctccaatca gcactacttt tgtcccatat tctttgacga ttccatttgc
    10261 tttttcatac gcatctgttc caatggtata atttggtaag aaaacacttt gactcatatt
    10321 catttccttc tttcttcgtt gaaacgggtt aaaattttaa agagtgatcg atctgactgc
    10381 ggctgtagat ggagtagagg tgaacctaag acaggataag tgatgaataa ggtggcatga
    10441 acaccttgaa atcatcaata agtatcctaa aaacgaacaa gtacaaaaac attgaaaacg
    10501 acaataatga atttttgttc ctcttagtat ttctaccgat tccctttgct tttttgtaat
    10561 tatctaaaaa ttctaaaaat taagattatt atatgaaaac aggttttatt tgtcaattca
    10621 taattggttt tttttaaagc aaaagataat ctatatcatg atgaacaaac agagtttttt
    10681 cctccaattg atggctttcc tgatctttcc tattttgaat aaagtgtttc tgtataattt
    10741 ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga
    10801 tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg
    10861 ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt
    10921 tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta
    10981 gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta
    11041 ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt
    11101 gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa
    11161 gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga
    11221 acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag
    11281 aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc
    11341 ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct
    11401 cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa
    11461 gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa ttccaactta tttccgaaga
    11521 agctactttt ggaacaccgt gtaccagagg ttgtgaaaaa gtcgcttagt ttcgagtagt
    11581 aagaaaaatt ttgtgaaaat ggctttctac atttttgcat aaatttggct taataccgag
    11641 aaactggctt ttgaacaccg tttattcaga taagggactg tgacaagact tttgtcacag
    11701 tttctttttg tttattcttt tttatctttg tatggcaata atagacaata taacgaaaat
    11761 tatttaatgg cttttggaag accatggaaa accttgtaca aagctatttt ttctgaaaaa
    11821 ttccttgaaa cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa aagtgttgat
    11881 tttttaataa ttattcatta aactcttaaa caacttcaga aaatattaat tattctgaaa
    11941 attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg gttgcggtat
    12001 gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt agttcagcaa
    12061 aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa gaaatgccta
    12121 cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat aacgtttatg
    12181 aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct agcgagttgg
    12241 cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac gcaaaatggt
    12301 caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga acagtagacc
    12361 cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca gaagacatta
    12421 cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac tatgaattag
    12481 aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc ccatcatttt
    12541 tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct gcaagtgata
    12601 aagctgttta taatggacca ttcacattag aagattttga tggaccagga acagatacag
    12661 aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa ctttctgaaa
    12721 tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa gatggccaag
    12781 cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat ccagcttata
    12841 cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa aagaattcac
    12901 cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt gaagctttag
    12961 taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca tcagatatgg
    13021 taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta gtttcttatg
    13081 acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc gttgattctt
    13141 tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc gaatatgttc
    13201 aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct gttccattct
    13261 ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc ggatgggctg
    13321 ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat tcatacaacc
    13381 gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca tcaacagacg
    13441 ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc atctctgatg
    13501 atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat gaaaaaatca
    13561 aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca tacgttgaag
    13621 aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg tcgcaacctc
    13681 gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga aagagcaatt
    13741 agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga cgagagcgag
    13801 gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga tcgctgaaaa
    13861 tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag aaacggcaat
    13921 gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt gatgagattt
    13981 tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga tttttttttc
    14041 atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac atctgtcaga
    14101 agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat taaaacgtgt
    14161 ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct tgatgcaatt
    14221 gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa tcgagatgct
    14281 aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt atctttctaa
    14341 tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag tcgcacattt
    14401 gttagctgga agaatcggac catccttgca gttagggtta caggcaatta tctttggaac
    14461 agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat gggcagatac
    14521 atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat ttgctgtttt
    14581 attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat gggatggctt
    14641 tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg actctgctcg
    14701 gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat tagcaaaagc
    14761 aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata gtttgattcc
    14821 attgatgact ttattaggac cgttagctgt agcattgatg accggatcac tggtggtaga
    14881 aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca caacaaatga
    14941 ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt ttgttatttt
    15001 gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag aagggagccg
    15061 aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc ctgcggatga
    15121 atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg caccttcttt
    15181 gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg ttatttcaat
    15241 ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt ctcctcatga
    15301 tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg gaatcaatat
    15361 cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat atgctcaagc
    15421 aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac gtgatgtatt
    15481 aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg ctgcattact
    15541 agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg gacgtgtcga
    15601 taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg ttgtcatgat
    15661 tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta tggccatcac
    15721 aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa aagatcaaga
    15781 atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat tcaaacatat
    15841 tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc catctgctat
    15901 tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt cagcttcatt
    15961 aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc tattatggat
    16021 tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg atggattgcg
    16081 tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt agaagtgaaa
    16141 gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg cggtgtcaat
    16201 tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag tggaaaatca
    16261 gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga tgctggtcaa
    16321 atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca aaaaatccgt
    16381 ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc gacgatgccc
    16441 attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa aaaagaggga
    16501 caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga gaaacgtatc
    16561 aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat cgcgattgca
    16621 ctggtatgtt atccggaaat actgattgct gatgaaccta caacagcgct tgatgtgact
    16681 attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga tacatcaatt
    16741 atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt cgctgtcatg
    16801 tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa cccgcaacat
    16861 ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa cgatcgattg
    16921 tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga cgcattctat
    16981 ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt ttttgaacta
    17041 tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa agtgacaccg
    17101 ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca tacttacggt
    17161 caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga aacagtattt
    17221 caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc acatttatga
    17281 aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta ctggccgtac
    17341 gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc aagatataac
    17401 gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga tgatctttca
    17461 agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg ccgaaggaat
    17521 tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg atgatctatt
    17581 gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat tttcaggtgg
    17641 gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt ttatcatttg
    17701 tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca atttgttgca
    17761 agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt tgtcgatggt
    17821 taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag agatgggaac
    17881 aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt tatctgctat
    17941 tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc aacctgaacc
    18001 agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg tctatgcaac
    18061 tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat ctcttctggt
    18121 tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta tccgacagaa
    18181 gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta taaccatttg
    18241 ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc gctcacaccc
    18301 gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc tttagcaaat
    18361 gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc cagcttccca
    18421 aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca aacccgtacg
    18481 atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat caagataaat
    18541 cagcatcggc cagttgaagg atcgattcgc tccgctacga tttctgctcg atataatgaa
    18601 gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc tttagctaaa
    18661 tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc tctagaagta
    18721 actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg gaaattgaat
    18781 gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag caattatcag
    18841 aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg agaagattat
    18901 cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt agaatctttt
    18961 ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt aataacaaaa
    19021 ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac cgacggagca
    19081 gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag acattcggtt
    19141 atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct tgttaataaa
    19201 aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc actaattatg
    19261 acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt
    19321 ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa
    19381 gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat
    19441 tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt
    19501 tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt
    19561 ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct
    19621 ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat
    19681 gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca
    19741 gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt gttactatga
    19801 cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct
    19861 ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta
    19921 ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt
    19981 gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat
    20041 gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct
    20101 tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct
    20161 ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa
    20221 tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta
    20281 aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa
    20341 atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa
    20401 agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata
    20461 acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa
    20521 tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt
    20581 gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt
    20641 ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa
    20701 gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc
    20761 ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa
    20821 aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc
    20881 gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct
    20941 attatccaag cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa
    21001 ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac
    21061 atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag
    21121 ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat
    21181 tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat
    21241 tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag
    21301 aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg
    21361 gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc
    21421 gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc
    21481 agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc
    21541 ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg
    21601 tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga tatggctccg
    21661 aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa
    21721 tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac
    21781 ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa
    21841 aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt
    21901 attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg
    21961 tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg
    22021 ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat
    22081 ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg
    22141 aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca
    22201 aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat
    22261 tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc
    22321 caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa
    22381 tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag
    22441 gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa
    22501 tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac
    22561 ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag
    22621 tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac
    22681 tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg
    22741 atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga
    22801 tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac
    22861 aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag
    22921 ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt
    22981 aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa
    23041 taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa
    23101 taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga
    23161 ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta
    23221 ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt
    23281 tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc
    23341 atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt
    23401 atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa
    23461 tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt
    23521 tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg
    23581 attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata
    23641 gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa
    23701 aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat
    23761 cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag
    23821 caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa
    23881 ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata
    23941 atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa
    24001 ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc
    24061 actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat
    24121 accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat
    24181 ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa
    24241 actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg
    24301 ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg
    24361 gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta
    24421 ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc
    24481 tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca
    24541 gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct
    24601 gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga
    24661 ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt
    24721 acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg
    24781 ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca
    24841 attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg
    24901 aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac
    24961 gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac
    25021 ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa
    25081 gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat
    25141 gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac
    25201 tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt
    25261 gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat
    25321 ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg
    25381 acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat
    25441 tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt
    25501 acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat
    25561 tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga
    25621 gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat
    25681 tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt
    25741 cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa atatatacag
    25801 tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt
    25861 acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca
    25921 ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc
    25981 aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca
    26041 aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga
    26101 ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt
    26161 cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt
    26221 acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga
    26281 accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga
    26341 agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg
    26401 ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc
    26461 gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc
    26521 tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt
    26581 tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt
    26641 ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa
    26701 gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg
    26761 ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag
    26821 caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac
    26881 aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga
    26941 aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa
    27001 gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact
    27061 gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg
    27121 atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa
    27181 gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc
    27241 tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt
    27301 agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt
    27361 acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc
    27421 atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt
    27481 tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa
    27541 acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat
    27601 ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt
    27661 cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag
    27721 acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg
    27781 gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag
    27841 taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac
    27901 agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca
    27961 agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa
    28021 ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg
    28081 caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc
    28141 ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg
    28201 atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat
    28261 ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta
    28321 acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc
    28381 atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt
    28441 tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca
    28501 acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct
    28561 tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat
    28621 tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta
    28681 gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat
    28741 ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat
    28801 aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg
    28861 gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat
    28921 tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga
    28981 tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg
    29041 ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct
    29101 gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt
    29161 tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc
    29221 ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc
    29281 actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc
    29341 aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat
    29401 aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc
    29461 aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa
    29521 tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt
    29581 aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc
    29641 tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc
    29701 ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg
    29761 ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt
    29821 gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt
    29881 catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc
    29941 cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc
    30001 cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa
    30061 ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata
    30121 ttttttatat acgtttttca gagccatttc taccataata cgttccacac tttctttttt
    30181 agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga
    30241 acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata
    30301 agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc
    30361 tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc
    30421 aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca
    30481 gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg
    30541 ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg
    30601 agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga
    30661 tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact
    30721 ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc
    30781 attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa
    30841 aagctaaaca aactgataat atgcgatata atacgaacgt attggataat ataaacaata
    30901 atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag
    30961 atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg
    31021 cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga
    31081 tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga
    31141 ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg
    31201 agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt
    31261 ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct
    31321 tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct
    31381 acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta
    31441 gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg
    31501 tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac
    31561 cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa
    31621 gaagtttagc gatccacgct aaataaaaaa caagcagtct tcccatccca gaagactgct
    31681 tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc atctttttga
    31741 agcagctcat catgggtgcc catttcgaca atagctccct gatccatcac taatatctgg
    31801 tccgcatttt tgattgtgga caggcgatgg gcaatcacaa aactggtttt tccttccatc
    31861 attgctaaga aggcttgttg gatttttttc tctgtcaatg tatcgactga gctggtagct
    31921 tcatctaaga tcaacatagg gggattactg atcatcgttc gagcaatcgt caacagttgg
    31981 cgttgtccat ctgatatctt taacccttga ccgccaatct tagtatctaa tttttccggc
    32041 aaacgttcta caaattcata catataagag gcttttaggg catcattgat ttctgcgtca
    32101 cttgcttccg gattcccaaa acgaagattt tctcgcaatg tactgtcaaa tagccaagta
    32161 tcttgtaaca ccattccaaa acttttacgt aatgtatcac gttggataga gcggatgtca
    32221 tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag caaattaaca
    32281 agtgtggatt ttccggcccc tgtttttcct actatagcaa tggtttgacc tggataagca
    32341 gtaaaattga aatcttcgat caaacgctgc ttggggtcat aagaaaaaga aacatgatca
    32401 aaagagactt ttcctttgat atttttgggt tccaaggcat aggcatctgg caaatcaggc
    32461 ttttcttctg gctgatcgat caaatcaaat gctcgttcta gaccagaaaa agcggtttgc
    32521 agttgtgtcg tgattccaga aagttcgata aatggtttag aaaactgact tgagtagatc
    32581 gtaaagctgg aaatcacacc aacagtgatt gacgaaccac tcttcaatgc caataggccg
    32641 ccaactaaac caatcgataa ataagcaagg tgatctacaa aacgagaaga cgggtttgtc
    32701 aaggaagaag aaaattgtgc ccgttgtcct ttttcatata gatcagcatt tagcagttca
    32761 aattgctgtt gactagcttc ttcttgctga aaagctttca cgattttctg gttaccgatt
    32821 cgttcggtca caaagcccga gatatcccca ataatccttt gctgtctatc gaaatttgtt
    32881 tgtgaagcac gagcaacgat ccaactcaca aggaaaataa ttggtgtaga acaaagaacg
    32941 accaatgtca gaagtggact tagacgcatc ataaaaataa acgaaagaat gatgattgcc
    33001 actccagaaa ataattgatt gaaggccgca gaaattgcaa tggaaatatt gtccatatca
    33061 tttgtaaatc gactgacgat atttccgtgg gagttttgat cataataacg aagtggcaat
    33121 cgattcagat gagcaaatgc atcttttctt aattttgcaa cggataagta agctaaccga
    33181 ttgcttaaca cttggatgag ccattgactg actacgttga ttaaaacgat accaccgaat
    33241 agaataagta aatggaacag tcgattgaag cggacctgtc cttctccaat catttgatcg
    33301 atactttgac caatttgtaa cgtcatcaca actgttgcaa tcccattgat gattcctagt
    33361 atcacagcta gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa tcggcggagc
    33421 gtctgcaagg ataatttttt tactttcata actattcttc ctcctgtgat gcgacgattt
    33481 cctgatattc tgctgattga gcaagtaatt cttcatgggt accctgagca gcaatcttac
    33541 cttgatccat cactaagata tgatgtgctt cttgaataga gcggacacgt tgggagataa
    33601 taatcactgt actgccaaga taagtctgta gcgccttacg caaatccaag tctgtttgat
    33661 aatccaacgc acttaaagag tcatctaaaa tcagtaatgc tggcttggca atcaaagctc
    33721 ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt tttcccattt tccatgactt
    33781 ttgtatctaa accatttgga agctgacgga caaattcttc tgcctgagcg attttcagag
    33841 cttcccaaca ttctttttct gtggcgtctt tcttacccca ttgaaggttt tcacgaatag
    33901 tcccggtaaa tagtacagaa gtttggggaa caagagcaat ttgctgacgg agatgatcca
    33961 gctgccaaga acgaacatcc attccgtcca ttataactgt accggaactg acatcataga
    34021 aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc ggttggacca atgattccaa
    34081 ggactgtatt tcgcttcagc tgaaatgtaa tattttggag agaaagacca gaatcaggag
    34141 tataacggaa gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa tcgggagtag
    34201 tactgacctc agcttcaaga atcgaatttt ctgtgtctag cacctctttc acccgatttc
    34261 ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga aacaacgatc agtgccaaaa
    34321 gcatttgatt catataattg atcaaagcaa gaacttgtcc ttgttgcaag ctaccaatat
    34381 ttactttgat cccgccaact gtcagcaaac agatgatccc gacattcatg atcaaggttg
    34441 tagcaggtga taatagggca gagatattgg aaacacgtaa ataattgttc gctagatcat
    34501 ttgtgctttt atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg cggatgacac
    34561 gaacaccgct aagattctga ctaacgtgtt cattgagctg atctagtttt tcttgcactt
    34621 tttggtagag aggaacggtt tttttgatga taaaaaataa aatgattgag aaaataggca
    34681 ataatgctaa gaaaaacaga cctacttccc agtcgatcac aaaagccatg accactgacc
    34741 cgatacttaa aaacggtgct ctgatcaata aacgaatgac cattgctaaa gctagctgaa
    34801 gctgattgat gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa tgattcaact
    34861 cgttgtgaga gaaagtattg atttttttga ttagctgatt acgtaactct gtaccaaatc
    34921 cttgtgaagc tactgaagca tagtattggc aaatgatagc acaaatcaga ccaatcacag
    34981 acatagcgac catccataag cccatttcaa tgatttttcc tcggtcattc atttttagtc
    35041 cattatcgat caaggaagcc atcattaaag gcaggacaag ttcgaagaca gcttcaagaa
    35101 atttgaagaa cgggcccaaa atgatttgct tacgatagtc ttttgcataa cgtaataact
    35161 gaatcatatg attcctccta ttatttattt gtccacaatt gaaaaaacaa gtactaccac
    35221 attgtagctt aggaaattta gaatgaaaag agaacaagcc ttttcataaa tgaaagtttt
    35281 attatgaaga agctttagaa attgaataga ctattttttt taaaaaaaag aaaatgagaa
    35341 aacatgccag tctcttcatt cataggaacg tctattttac ttaatttttc tataaatgaa
    35401 ttctgcataa aaaatatatc aagtaaatgt ttatagatga aactctattt acttcttttt
    35461 taatttgttc ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg gttgagagaa
    35521 atctaattga ttttcaaaga aggagtatga aaatgagaaa aatatttatt cgtttattgt
    35581 gggtactatc ttagaggaat aaataatagt ggagaaaact atggtttggc gcagattcta
    35641 tcaggtttga tggaacgaag acaacctccc gacatcaagt caaaagctac ttgagatttt
    35701 gatctgaaaa taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt
    35761 aatcaatggg aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag
    35821 tacctcttta ctctctgaaa gcatcataac tcgtttttcc tcattatgcc aataaattgt
    35881 tgttatacca catataagga tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt
    35941 gaatcttatt ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa gatgaataaa
    36001 aatcctgttt cggctatata ttgcgtatct acgccaataa aacgagtttg ttctgtcata
    36061 gccatataaa gcaaaggcaa aaatacaact acagcgagat aagatccgat gatttctcga
    36121 aaagttttcc aggaagtatg tttcctgttt ttatatttat ccgatttcat tgaatagtca
    36181 tagcctcctt attctttcat atgttgaaat tccagtataa aaactggaag gaactgtacg
    36241 ttttttagaa taaaaattat aagggataag taggctgctg tctataataa gatatatttt
    36301 tcaattcttt attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg
    36361 gtgaataagt tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc
    36421 gatttagaac atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta
    36481 aaaagaaaca cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa
    36541 atcaattctc gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag
    36601 ttacgcacga atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg
    36661 caaccaaaag acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc
    36721 gaacaaatca aaacagctct ttactatcca gatggcggat taccactttt gattacaggc
    36781 gaaagtggca caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat
    36841 gatctgctag aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat
    36901 cctgaacttt tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa
    36961 gaaaataaaa aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta
    37021 catcgcttaa caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt
    37081 tatcgtatgg gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg
    37141 actgaagaga ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc
    37201 gaactacctg cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt
    37261 tttctcgaag aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca
    37321 cttttaacaa gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt
    37381 tcagcagcaa aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat
    37441 catttgcaaa aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc
    37501 tatattacgc aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag
    37561 cgaatcgtta aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta
    37621 tattcttgcg aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt
    37681 gagaccagta gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag
    37741 acctttcgac aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc
    37801 ttgagttact atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt
    37861 atccaactaa tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac
    37921 tatgtccaac gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg
    37981 gatcggattt tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg
    38041 ccaaaagcga tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta
    38101 aatcgtttat tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca
    38161 cagcagattg cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg
    38221 atcatattag tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta
    38281 tccgtaccgc ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa
    38341 tgcctgcaaa aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag
    38401 ccagaatggc gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc
    38461 ttcactggaa tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg
    38521 gcttgtcagt tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt
    38581 gacccattat tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc
    38641 accaatatcg actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta
    38701 gctgaatggt tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc
    38761 attcgtaatt tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag
    38821 gtgatgggag aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc
    38881 agcaatccca aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc
    38941 cgacaaatgc caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa
    39001 gggttatctg ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc
    39061 cccaattctg agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg
    39121 atagaagaag aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc
    39181 ttttttagta tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag
    39241 cttcccatgg aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa
    39301 atcattctat tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag
    39361 taaggcagct ttttatgagg tacaaagata aagaaatcat tgttctgacg gatctttttg
    39421 gcggaagtgt caacaacgaa ttcttacaat atatcaatcg ggatcatgtc tatctcgttg
    39481 ccggattgaa tttgccacta ttgattgagc tagtatctcg gttggacgct gaagtagaga
    39541 caactgtttt gatccagcag gtgctaactg attcaaaaaa aatgatccag ttttgtaacg
    39601 agcggctcaa tcagaaaata gaagaagacg aattttagga ggaagaaaaa tgattgtatt
    39661 gacaagagtc gaccatcggt tattacacgg acaagtagct ttttcatgga cacaaacaat
    39721 aggcgcagac tgtattttga ttgctaatga tgatgtacct acgaatgaga tacgaaagac
    39781 aacgatcaaa ttagccaagc cacaaggagt caaactagtg atcaaatcaa ttgatgactc
    39841 gattgccgcg ttgaaaagcg gggtgacaga taaatataag ttattcatcg tagtggaatc
    39901 gatcgaggat gcatataagc tggcagaagc gtatccagaa atcaaaacca tcaacttagg
    39961 agggatcaag gcaaaacctg gtactcgcag catcggaaaa gcggtaaata ttttaccaga
    40021 agaggaggaa cgcttgaaac gattaattga caaacaaata gaagtagaaa tccgtcaagt
    40081 accagcagat aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga acctcaatga
    40141 ttgtacaagc agtcttatta ggaattgttg catttgtcgc acaatcagaa tatgcactag
    40201 ggacatcgtt gctttctcgt ccgattgtga cggggctgtt tacaggaatc gtgttaggtg
    40261 atgtaaaaac tgggatcatt atgggcgcaa ctttagagtt agcatttatc ggatcgttct
    40321 cagtaggtgc ttcgattcca cctgatgttg tgaccggtgg gattttaggg acggcttttg
    40381 ctattacagc tggagccggt acagaaaccg cgcttttact aggtttgccg attgctacgt
    40441 tgacattgat attgaaaaat atttacttag ggcttttgat tccaattatg aaccataaag
    40501 cagacaatta tgcagaagaa ggaaattata aaggagtcga gcatatgcac ttgcttgccg
    40561 gtatcggtct ttccttgatg cttggtgttg tggtaatggt ttcttatatg gtgggaagca
    40621 atacgatcgg aaatctatta aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag
    40681 ctacagggtt gattccagct cttggttttg cgatgcttgc tcgtttgctg atcaacaaac
    40741 aagttgcacc atattttttc ttaggttttg ctatagcttc ttacttagag attcctgtga
    40801 ccggagtagc tatttttggt gcgattttag cagttgtggt agtcaatatt atgaatgtcc
    40861 gtcaactacc acttcaaaca actgcagggg aggtagaaga agatgaagaa ttctgagatc
    40921 aaaaacagca aaataacgaa aaaagagcta aatcacgtat tttggcgttc ttttcaaatg
    40981 gagttttctt ggaattatga acgtcagatg aatatggctt atgtttatgc aatgattccg
    41041 attttgaaaa agttatatca gtcgaaagaa gaaatggcag cagctttgaa acggcatcta
    41101 gagtttttta acacgacccc acatatcgtt acactgattt taggaatcac ggctgcaatg
    41161 gaggaagaaa ataaggatga tcctgaattt gatgtgactg cgatcgatag tatcaaaaca
    41221 tctttgatgg gacctcttgc agggttagga gattctttct tttggggaac tttgcgattg
    41281 attgccacag gtgtgggtac ctctttagcc atgcaaggaa atattttagg ccctatcttg
    41341 ttcattttga ttttcaatat tccgcacatt ctgtttcgct atctagcaac aggttgggga
    41401 tatcgcttag gaacgggttt tttgaaaaag atccaggcaa acggtatgat ggaaagtttg
    41461 acattaggcg cgtctattat tggactgatg gtcgttggag caatgactgc cacgatgatc
    41521 accatcaata ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt
    41581 tttgatgata tcgttccaaa tatcttatgt cttggagcat ttggcatagt gttttatcta
    41641 ttgaagaaag aagtgaaacc attaacgatc ttgatcggac tagctttctt tggaattttt
    41701 ggttcgtgga tagggatttt ttaaaaaagg agcagagaaa atgtttacga tgcaagatta
    41761 tatctatgaa gaaaaggaag tactaagtac gatcttgaaa aagaatgatt tttcgacaag
    41821 agaacatatg aaaaaaacag tgaatttact gatcttagca acgggttcat cttataacgc
    41881 ttgtttagca gcgaagccag cattggaatc ttatggtgat cttactgtcg atattcaaga
    41941 acctttttat tttttcaatt atggaaaact ttctccttca atcgatactg tgattgctgt
    42001 gtcacaaagc gggaaaagtg cttctactgt tgaagcagta aaaatgatac aaaaacaagg
    42061 gttacctgtt gttgctatca caaatgacgt gcaaagtcca cttgctttag aagctgcaca
    42121 aattattgat ttaggtgccg gggtagaatc agtaggcttc gttacaaaag gctattctgc
    42181 gactgtttta cagttactac tcttagggat aggaattgga ataagtaaaa ataaaatcag
    42241 caaaaaaata gaacaagact acatgcaaca gttgcgcaaa ataatcaacc atttaccagc
    42301 gattattcaa aaaacggaag aattttttga tggatatcaa agtttatttc gtttagcaca
    42361 gcgtttcgtc accattggat atggtccaaa ctggggaaca gctaaagaag cagagacgaa
    42421 attgactgaa acaatccgcg ttccgtcaca aggatttgag ttagaagctt atatgcacgg
    42481 cccatacctg gaggcagatg cttcgcattt gctattcttt attgaagggg aaagtgtaaa
    42541 taaggaacgt tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag agactctcac
    42601 cattaccaca aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag aatgtgatga
    42661 atatctgtct gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt
    42721 agctaaaggt attgacttga acaaaaagat ttttgaagac tttgatacag tcttgaaaag
    42781 taaattataa gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat
    42841 aaaaggcgca ctagctttac gtccgcaagt agaagaaatc atcgataaac tttatgcgga
    42901 aaaatttgac gctgtctatt atctaggaat cggtggaacg tatgcttcct cgatgcaagc
    42961 tgtcacttat atgaatggga aaagcaactt gcctgtcttt gtacagcatg ctgcagaata
    43021 ttacactact ggaaacaaaa gattaacaaa agattctatc gttattttgt cctctgttac
    43081 cggtacaact caagaagtag tgaaagcagt agaacaaatc aagaaagtag gagcgacact
    43141 gattggattt atcgataaag cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac
    43201 ttatccagct cctggaacag aacaaatcaa attttttatg gcagcagatc gattgatgta
    43261 tttgaatgga gaattcgaag cttattcaga atactatgaa cagctcgaac gttatttacc
    43321 aacagggtta gtggaagcag agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa
    43381 acatcgacgt gatagtatgc attattttat tggagcaggg aatcaatggg gagctgttta
    43441 ttcatatgcc atgtgttact gggaagaaca aagctggctt ccatcgaaat ctatccatgc
    43501 tgccgagttt cttcacggga cgttagagat cgtagaagaa acaaccccag tcacattatt
    43561 tttaggcgaa gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat
    43621 ttgcagcaat tatacattga tagacacaaa agactatcca gtagaaggta tcagtgaaaa
    43681 atatcgcgga cgtgttttgt ctttcttatt gatgcatgta gtgacacaac gcatcgatgc
    43741 tcatgtggag cagttgaact gccatccatt agagatacgc cgctactatc gtcagtttga
    43801 ttattaggat agactggata aaaagaatat aaaaaataaa gagagcagcg ataactgctc
    43861 cctttatttt tttaaaatat tggcatcaat tacgttataa aacacatttt ttgcattatc
    43921 tacagttcaa cttataatct gtttaatggg acatgtgggt gccaaccatt ttttgttatg
    43981 caatagtact atttagttgt tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc
    44041 gccagtttac atagcgtttt gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa
    44101 ccgtcaacag agacaatctg tccgttgcag gacctgacag atttagaaat gttcaatctg
    44161 tctcttatgt attttaaatc aatactcact aggagaagtt tcctaacctc ctaatagtaa
    44221 tgctggtagt aacattgtca aaactgcttc ctcttaggaa caagcaaagc ataacgagtg
    44281 taaaaataaa atgtagacta ttgtaacata tcattgatta tttcaagtaa aaacgataaa
    44341 agaagtaaac aaacacttaa aaacaatgga aattagtccc aactttaaga aatgtatact
    44401 ataatggaaa ttatcaatta tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt
    44461 cttggtcctg gatcttgggg cactgcttta gcacaaacat tggcagaaaa cgggcatgat
    44521 gtccgtattt ggggaaatgt acctgaacag attgatgaaa tcaataagta tcatacaaat
    44581 cagcattttt tacctgattt gacgatccca gaatcgatta tcggatataa agaattggca
    44641 gaagcagtgg ataacgcaga tgctatttta tttgttgttc caacaaaagc gattcgttca
    44701 gtcgcacagg aacttattgg taaaatgaat actaagccaa tcattataca tgcaagtaaa
    44761 ggactagaac aagatacgca taaacgaatc tcagaagttt tggctgaaga aattccagaa
    44821 gaaaaaagac aggcaattgt tgtattatct ggtcctagcc atgctgagga agtggcagta
    44881 catgatatca caacaattac agctgcaagt attgatcaaa aagcagcagc ctatgtacaa
    44941 aaactgttca tgaatgatta tttcaggatt tatacaaata atgatgtgat tggcgtagaa
    45001 actggtgcag cactgaaaaa tatcattgca attggtgccg gcgctatcca tggattagga
    45061 ttcggagatg atgcaaaagc agcaatcatg actcgaggat tggcagaaat cagccgtctt
    45121 ggtgtcgcga tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt gggggacttg
    45181 atcgttactt gtacaagtgt tcattctcgc aattggcgtg ccggtaactt gttaggacag
    45241 ggacataagc tggaagaagt tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg
    45301 acaaaagcag cagtagagct agctcaacag ctaggtgttg agatgccaat tactcaaaca
    45361 atctataatg ttttgtataa tggagaagat atcaaaaaag cagcaaaaga aatcatgtta
    45421 cgtgacggaa aaatggagaa tgaattttct cttcgttaaa tagatgatca atagttaatc
    45481 gtcgtttagg aggaacagca agtgaaggtc aaaaaagcag tcattcctgc cgctgggcta
    45541 ggaacacgtt ttttgccggc aacaaaagcc atggcaaaag aaatgcttcc aatcgtggat
    45601 aaaccaacga tccagtttat tgtggaagaa gcattggatt cgggaatcga agacatcttg
    45661 atcgtaacgg gaaaagaaaa aagacccatt gaagatcact ttgatgcaaa tatcgaattg
    45721 gaaaacaatt tgcgggaaaa ggaaaaaaca gaattactcg ctttagttga agaaacgact
    45781 caagtcaatc ttcacttcat ccgtcaatct catccaagag gactggggga tgccgtgctt
    45841 caagcaaaag catttatagg aaatgaaccg tttgtcgtca tgctggggga tgatttgatg
    45901 aaagatgata tcccattgac tcaacaattg atgatggatt atgaagaaac acaagcttcc
    45961 gctgttgccg tcatgcgtgt accagaaaat gaaacatcta aatacggaat tattgatcct
    46021 aaaacagata gcgaaaaggg tcgttgcaga gtaaaagggt ttgtagaaaa acctgaaatg
    46081 ggaaaagcac caagtaattt agccattatc ggacgttatc tactgactcc taaaatattt
    46141 gagatattag aaactcaaga accaggagca ggaaacgaga tacagctaac ggatgcactt
    46201 cagactctta accagacaga ggcggtctat gcgagagagt ttaaaggaaa aagatacgac
    46261 gtaggcgata agttaggata tatgaagaca aatattgaat atggacttca acattcggaa
    46321 attgggactt cattaagtgc atatattatc cagttgagta aaaagctaag aacagaaagt
    46381 taactggaaa taagtggcct gctgagaatg aaaggctatt tgttattaaa gcggcagctt
    46441 gtgcgtcatt tgcgcaactg tcgttttagt tttttgactc tagatcagtt gagtcgtgga
    46501 aaacgaaaga tagaaaaaga ctttcgtgaa tagattcaga aaatattatg ctaatggttt
    46561 aatttttttt taagttgtat aaaagaaaat gattacataa gagaaatgaa agccaagcca
    46621 gaagattcat agtagaataa aaaggcgaat tttttaatta taaaaaaagg tgtgtgttca
    46681 ctatgggaaa attagtcgct gttgcacagt cgctgcttga tatactcaag tggctgatgg
    46741 aaggaagcaa ttggatattt gtgttgccac tattgcttat tagtctattt atcatgtttc
    46801 gcatggtcag aaaaaaagaa acgtttctga tttttatctg gtatgcagca tccgttgggg
    46861 ttgctacctt gatggttgaa ttggcgttag gtagttggga agcttgcgta tttgctttat
    46921 tttttgctgt actgtatcta atcatttata tcccatatat gaaaagaaag tctcggatag
    46981 gcagaaaagg tagacataca actacttttt ctagtacaga ttctctcgaa caaagtagaa
    47041 gaacgagaag aagacggtaa tatttagtag gtatacgcaa aaaaagataa aataatggat
    47101 aaactattga gatgagagat ttatttaatt ggtggaaaaa agcttttttc acttatataa
    47161 aagaatcttt tttaaaggaa tgcttgttgt atcgggataa gccattcctt tttttatgtt
    47221 atttatatat atataaatca taccaatgtt tttttgtatt ttcttgcttg cacttggata
    47281 agagattact tacaataaat tcagatagaa acagctgaat aaaaaaaggc tccctgaccc
    47341 ttagttggcg ctaagaatca gggaatgacc agtcacacac atacaccctg accgatcatt
    47401 ttgccatagt caatgcagtg catcgaccta atttattgta cctaatctag ggtgaaaatt
    47461 ctacccctat gtagatattt tccatggtct gcacgttgtt ttatttgcta tgtcaaggta
    47521 ttgggcgtat tcgttcaatg ccttttttgt gtttgaaaca gcaaagacca catgtgttac
    47581 tgtctaacta ctgttttttt atgtacttcc ttagattagg cagctgtttc tattcatgtc
    47641 aagtatagct aggcgggaga gataaatgag tttatgttat tttgactatt atcaccaatt
    47701 ggaagagaaa ttaaggatag aagagccgct gatgcttcaa gctggcactt ggatcgcgaa
    47761 acaaattaat caaggtggta gattgcagat atatgctagc cgcacactaa gtggtgtagc
    47821 atttgaattt tgggatcaaa ccccggatct tgtaccaagt atattgattg aaaatccagc
    47881 cggaggtgtc tatgaaacgc tagaaggtgc aggacaggca attattgatc agctttctgt
    47941 caaatcagaa gacatttttc ttcttctttc gaatgaagga agaaacccgg caatcatcga
    48001 actagctcag tggatcaaag aaaatggtca tccattaatc attgttactg gatttgattt
    48061 gtcacgaagt atcaaatcgc aacatagcag tggtttgcga ttatttgaat ttgctgattt
    48121 ggttttggac aaccacgcca atttacatga tgcagtcttg actttgccaa atctagatcc
    48181 agcgatctgc ggaacagcct ctcttgcaac gatctttttg ctgcagcaag ttttgtattt
    48241 tacagcaaaa cagctagtcc aggacacaag tggaaaatag ttatccacat gttgacaaag
    48301 ttccttaaaa aaataatgtt ccacataaaa atggagcgtt tgaaaaaaag aaaacgatta
    48361 ttaatgagga ttaacaacga ctactttttt tgttttagtt attttaagat ggatcatctt
    48421 tgattttaag cttgttttgt ttaaagtttg ttatttttat aagttgtttc acagttatga
    48481 gaaatgagtt ataagaatat gtagtgtcag aaaaataaaa agtagtggaa aaagagacaa
    48541 gaaagaatcc atatggattc tttcttgtct cttaatacac gttgatgaaa gagtcttccc
    48601 caaaactctt tacaaagtaa ttatagtaca tttgttatca ttattgcaat gaagtttacc
    48661 tgatataaca aaattgtcca acaggatacc tttttctaat agaaataaaa tacacaaaaa
    48721 ttgaaggata gataatggat atttataagt gtttattatt ttttttgcaa tgaaattcaa
    48781 tttttcaatg attgtaaaat gaaaaaaaca ggacgagatt ttctctaatc taaaaataaa
    48841 aaaagaattt taaggaaaaa cagagaaata aaatacttaa tataaatgga aagagaaaaa
    48901 aataatcata cataggagaa aaaaaacaaa atagaaaaaa gtgattttct aataaattat
    48961 tcatgtggaa atgtcatttt tactgtataa ttcatttgat tatccatttt ttattcatca
    49021 aaattactga aaaaagaaag gaagaaataa atttgaagaa aacaaattct tcaggaaaac
    49081 cgaaacgcga cttcactaaa ttatcgacac cccatacgta tgtcattatt tttggtgtag
    49141 tcatctttgc atggatactg acttttgtcg ttccagcagg aaagttcagc acgcaagata
    49201 ttgaatacaa agatgcaaat ggcgaaacaa gtactcgtac tgttttacgt caagattcct
    49261 tccgttatgc ttacgagctg gataagtcgt atgtgtttga tcaattagaa gaactgcaag
    49321 atcatccagc agaacgtgaa aaactggatg ttccggaaaa aggattggaa aaagtcattg
    49381 ctgacggaga aaaaaatctt acgcaggaaa aactagatga aatctccttg acagatgatg
    49441 tgctttacga tcagtacggc gaaaatatct atgatacttc taaaaaattg cataaaacag
    49501 ccaaaatatg gggaacagat gattttggcg gctttggttt cttaaatttt gtttttgaag
    49561 gactagtatc tggagataag tatggatctg ctgtagggat cgctgcattg atccttgttg
    49621 ttggaggtgc ttttggtatt attatgcgta ccggagcgat cgacgcaggt atctatgcgt
    49681 ttatcagtaa aactaaggga ctggagcgtt tagctttacc gctattattc tttgcttttt
    49741 cattcggtgg cgctacattc gggatggcag aagaggtaat tccattctca atggtgatgg
    49801 ttccatttgt cattgcgtta ggatatgact cgatcgtagc agtaaccgtg acttatgtcg
    49861 catctcaagt cggaaatgcg acctcttgga tgagcccgtt tagcgtagcg gtggctcaag
    49921 ggattgctgg aatccccgtg ttgtctggtg cgacattccg attgattatg tgggtagttg
    49981 ttactgcatt agccgctggt tatatgatga tatatgcaga aaaaatccgt aaaaaaccag
    50041 aaagttcgtt gacttacaaa tcagatgatt atttccgttc tcatatcaaa aagacatctg
    50101 atgaaaataa aaaattcatg cttgggcata aattgatctt attagagatg ttagttgtcc
    50161 ttgtctggat cgtttggggc gtgactcaaa aaggctatta cattccagaa atcgcttctc
    50221 aattctttgt gatgggatta gttgctggaa tcattgctgt tcttttcaaa ttagatggaa
    50281 tgaaagtcaa tgacatcgct tcttctttcc aatctggtgc agctgattta gctggtacag
    50341 caatcgtagt aggtatggcc aaagggattc ttcttgtctt gggaggatct gatgcatctg
    50401 ttccttcagc tttgaatacg attttgcatg ggatcgggac ggcgttgacc ggtgttccag
    50461 ctgtcattgg tgcttgggcg atgtacatct tccaaagttt gttcaacttg gttgttacat
    50521 caaattcagg acaagcagct ttgactatgc caatcatggc tccactggca gatttagtag
    50581 gtgtatccag acaagtagca gttcttgctt atcagcttgg ttcaggtttt atggacgctt
    50641 ttacacccgt atcagccagc ttgattggtg tgctaggtgt tgcacggatc gaatgggcaa
    50701 aatgggcaag attccaaatc aagatgcaag gattcctgtt tgttttagga actattttca
    50761 tcatgattgc cattgcaata ggtctgcagt agtactggag acctgtaaat aaaaaagtaa
    50821 tccaaacagt agataaggac tccaagaacc gtcattttac ggtaaaatca aaggagtaaa
    50881 gatcctatcg tttggattat tttttaagat cctatcatca gttatactta aagaaagagg
    50941 aaaaatgtat gaaaatcatt cgacaaattg aagtgtttgc accagattac ttaggaaaaa
    51001 tggatgtact gattgccggt gggaagattt tggctgtaga agaacagcta gaaggcggct
    51061 acgaaggagt ggaagtagaa gaactttcag gtgagggaaa agtactaacc ccgggtttca
    51121 ttgactgtca tttccatatt ttaggtggag gaggagaagg cggttatcaa aatcgaacgc
    51181 cagaagtcac attgagccaa ttgactacag ctggtgtaac gacagttgtc ggttgtttgg
    51241 gaacagacgg agaaggtaga gatatgactg cgttgatcag caaagcgaaa ggtctggaag
    51301 cagaaggaat ctccacttat gtatacgaag gttcgtaccg attgccggta aaaacagtaa
    51361 cagattctat catcaaagac tttttgacaa ttgataaaat tatcggaatc ggagaaatcg
    51421 cagtatccga tcaccgttct tctcagccaa gttttgaaga gttcgctcgt gcagtagcag
    51481 atgctcgtgt aggaggcatg ttatccggaa aagctgggat catcaatgtc cacttaggcg
    51541 gaggaaaaag aaagctagag ctattgacac gaatcgtaga agaaacagaa attccgatca
    51601 cacaattttt gcctactcat gcaaatcgca caccagaatt atttgaagct tgcgtggcat
    51661 ttgccaaacg tggtggcacg attgacttca ctgctagcga agatcctgat ttttgggaga
    51721 agacagatgg tgaagttcgc ttcagcaaag cactgaaacg attgatcgag gaagatgttt
    51781 ctttagataa tttcacgatg acctcagatg gacaaggaag cttgccttat tttgatgaaa
    51841 acaatcattt cctagggtta ggagtaggaa gtgccaaggc attgctagta gggatcaaag
    51901 aagctgtgca aaaagaaagt atccctttgg aaatcgcctt acgagctata accagtaatc
    51961 cagcacgtat attgaaattg gataaaaaag gaaaaatcga aattggtgct gatgcagatc
    52021 tgtgtatctt ggataaagaa acgcttgata ttgatacagt tatcgctaaa ggagaaataa
    52081 tggtccaaga aaaagaagtc aaagtatggg ggactttcga aaaaagtttt taatcaacag
    52141 ctctaactat cttttttaga aaacgagcgt tctgtttgtc tgtatctgtt gttgatgtta
    52201 ccctgaaggt agatcaataa acaaactaag aggaggaagc acaatggcag atttaaaagg
    52261 acgttctaaa gatatgaaag ataaagtagt cggtgaagga aaagaagcat acggcaaagc
    52321 aacagacgac aagagcaaag aaacagaagg gaaagcacaa tcactaggtg ctgacatcaa
    52381 agaaaaagct cgaaatttag gcgacgaagt caaagacaag ttttctcgaa aagacgaaaa
    52441 aaaataaaaa ccacttatta ctaattgaat caagcaaata aaaaatccga accatatctg
    52501 cgagacagat atggttcgga ttttttattt gatcattatc ctgtttttta aacttcgatt
    52561 ttttgaaatt ttttattttt tagttcaaag acttcatcac agctttttgc tacttcaatc
    52621 tcatgagtca cgatgataat acagcgattt tgttcatggg cgatttcttg gaaaagtttg
    52681 acgatatctt gtgaagttgt ttcatctaga tttccagtag gctcatccgc tacgattagt
    52741 tgatgttcac agcagatagc acgaacgata gcgactcgct gctgctgacc tcccgaaagt
    52801 ttggtcacct gtttttttgc taatatttca tcaattccta cacgagccag tcgatctaaa
    52861 gcaacttgtc gataattctc ttgcttggtc ttagtgattt ccattgctgc tatgacgttg
    52921 tctagagccg ataaataagg aagcaagttg taagcttgga aaataatcga gacatcattt
    52981 ttacgataac ttcttaaccc gattttgttc agtgaacgat cttgatagta tacctttcct
    53041 tcttttggtg catctagtcc agaaataagc gaaagaaaag tcgtttttcc agaaccactt
    53101 gctcctaaaa tagcgtacat ttttccagct tcgaaattca gttctacatc tttataaagc
    53161 gcatcttgcg gatcttgata ccaaaatccc aattgttctg tccgtaacat caaattcctc
    53221 ctttagttta ataggatttt tcttgggttt aatcttaata tccctataga agctaaaaag
    53281 acagaaaaca gactgattcc aaatgctact ccggctaata gtcccaattg tgcaggtttg
    53341 acattgattt ccaattcgct gacttgatca gaaacagcaa atggattatt cgtggttcct
    53401 tgttgaccat cgcgttgacc gccttgttct tgtctgttac ctggcatttg tccaccgcca
    53461 ggagcttgct ggttttgtgt cgtggtttct gtctgttggt caagtagctg ctgtcccacg
    53521 gcattcgcga cgacgtttcc gctaagcgaa gcaactccta atgcaagaat catacaaata
    53581 gccacttcgg taaacaattg gagaatgatt ttacttctgg attctcctaa agatagcaag
    53641 acgcctaact catgccgacg ctcccgaatg gtaatcataa tgatcagagt aagtatgata
    53701 ataccggcta cagccactag taaaacaaca tttttagaaa aacttgccac gttgtttaaa
    53761 ggttccaaca ttgactgata catggaatca tttgattgta agctaaatgt atctgtgtcg
    53821 atcagcttct cagcttcttt gagaaactca tccattttat ctggatcaga tagagtgtaa
    53881 atagcagaat ctatcgtgtt atcgtcagat gtcccattta gttcatttgc aaaagtgtaa
    53941 gaagtgtaaa gtgtattcga cggattcatg aagttgaatt tcatacccat actgccgctt
    54001 gtttctctgg attcataaat tcctttgatc gtcatttcat aagtggtatc ttcatcttca
    54061 gtactcgtga ttgtgaaagt atctccgact gccaggtcat ttgtttctgc tagagtagaa
    54121 tcaatgacta cattgtttgt gcctttgtcg ctgtcatcta ttccttcacc atccgtgatt
    54181 tttgctgttc catcagaaaa attactattt tgtgcagttg ctgacacacc actgatttga
    54241 aagtctgctt gagtcatttg cggattcccg cccattcctc cagcaggttc accgcctgca
    54301 tcagtagttt ctgaactgtc ttcacttgaa tcagaactag tgatggcagt tattccatct
    54361 tttgccatag ctgaggcgga actttcaaag gaataactct ttacattatc taattcagct
    54421 attttttccg cgtccgttaa ggaaactggt gtaagagaaa aactgcctgg atctggacgt
    54481 tcttcagtgg tagaaccgct tgaagaagtt tgttcctgtt ttttaaatga agcttctcga
    54541 ttggcactta aagtcactgt tgctccaact tctttttgtg cttgattcgc tgcttcttct
    54601 gcagcgcttc gaatcgtgag gccagctagc acaaaaataa gaatggctgt gaacacggca
    54661 aacaataaca atgaacgtcc ccattttact tttgtacttt tccatgcacg tttcataaaa
    54721 ttcatatttt gcatccctcc tagtagatgc atttgataat agacaaccta tcttaaagct
    54781 agcttaaatc ttatgctgtt tgcacaccat caacatgagg ttctgtatat ataaaaagtc
    54841 actatagtta ttgcaaaaaa tgtggtaaaa tgaaaaaagt atgtttcaaa ccgacgaaga
    54901 agaaatgaaa gaggaatagc aaaagaaatc ctagatgcta cagagggatt tttacgtgca
    54961 tagttgttct aacaattgct gatactagcg atttagttta tttgttgtat aataatgggg
    55021 aacagctgaa tacttatata agtattctgg ctatagcgtt tcgcaaaaga tccattttgt
    55081 taatgaagta tatccaaaat aaaatgccca tccaactgtc agaacgcgga tgtgctggta
    55141 attttcctgc tgattctcca taagttgaga aatagtgatt ttgcatatgc ttcattggaa
    55201 tggctcatat atttagataa agaagaaaat cgacatcttt aaacttggat gtcataagaa
    55261 gaggtgaagt ttgtgagtac cattaaaatt atcccattag gcggcgttcg tgaaaatgga
    55321 aaaaacatgt atgtcgcaga agtgggcgag gatattttta ttttagattg tgggctaaaa
    55381 tatcctgaaa atgagttgtt ggggatcgac gtcgtaatcc ctgattttac gtatttagaa
    55441 gaaaacattg atcgtgtagc tggtgtcttt ttaacacatg gacatgcaga tgcgatcggg
    55501 gcacttcctt acttgttgtc aaaaatttct gtaccagtat tcgggacaga gttgacgatc
    55561 gagttagcaa aattgaatgt cagtcgtaat gaagcagcca aaggatttaa ggatttccat
    55621 gtggtagatg agcatacaga aatagacttt ggtgaaacag tagtgagctt tttccgtacc
    55681 actcatacga taccagattc agtggggatc aatctgaaaa caaaagaagg tagtatcgtt
    55741 tatacgggag attttaaatt tgaccaaacg gctatcccaa tgtatcaaac agattatgca
    55801 cgattggcgg aaatcggcaa agaaggagtc ttagccttac taagtgaatc ggcaaatgca
    55861 gaaaatcctc agcctgtagc ttctgaagcc caaatagcag atgaagtatt tgatacgatc
    55921 aaatattggg aagggcgtat catcgtcgct tgtgtagcaa gtaatttgca acgagtgcaa
    55981 caagtattgg atgcagcaga aaaagcacaa cgaaaagttg tcttgactgg tcaagatttc
    56041 gaacgaatta ttcgcacagc gatgcgttta gaaaaacttc aattaccaag tgaagatctg
    56101 cttgttacgt tgaaagacat gaaaaactat gatccagaag aactgatcat tttagaaact
    56161 ggccgaatgg gtgaacccat caagtctctg caaaaaatgg caaacggtac acaccgtaat
    56221 gtcaagattg aagaaggaga tttggtttat atcactacta cgccaagcat cgcgatggaa
    56281 accatcgtgg cgaaaacgga agatatcatt tatcgagctg gtggaacagt gaagttgatc
    56341 tctgagaata tgcgcgtttc tggccatgcc aatcctaatg acttgcaatt aatgatcaat
    56401 ttgatgaaac caacatattt tgtgccagtt caaggagagt atcgtgaatt agctgctcat
    56461 gcagatttgg ctcatgcagt agggatgcca tacaaaaata tctatattac tggacgcgga
    56521 gacgtgctgg aatataagaa caagcgtatg agtgttgcag gaactacaag tgcagaaaat
    56581 gtaatgattg atggattagg cataggtgat atcggaaaca ttgttttacg tgatcgaaaa
    56641 atcttgtcag aagacgggat tttcgtagcg gttgtcacta tcagtcggcg agaaaagaaa
    56701 atcatttcaa aacctcaaat tacttcacgg ggattcgttt acgtaaaagc tagccgtgat
    56761 ctgatgaaag aaagcagcaa tatgattgaa acaatcgttg agaaacattt agaaagcaat
    56821 gattttgagt ggagcaaatt aaagcaagac atccgagatc aattaggccg ttacttattt
    56881 gaacaaacaa aacgccgccc agtgatcttg ccagtcatta tggaagctac tcaacgtcga
    56941 aataaaaaat aaacaaaaag agtaagatga acctctctat agagtgctcg tcttgctctt
    57001 tttttgcgaa aaacaaataa tttttgaata cagcatccct cgctttataa taaacgatag
    57061 aataaaggga gtgaatcaat gatgagtcaa acaaaagtcg aacgttattt agaagaacaa
    57121 aagatcagct atcaaccact tgattttagt catgtggtag aagaatcttt cacagaagaa
    57181 ctacaaaaaa gagggattga tccagctttg atatgtaaaa ctctcgtagt gaaaggaaac
    57241 aaaactgggg tgatcatcgc attgattcct ttgaacgatc acttagatta caaaaaaacg
    57301 agacaagtga cgaaagatcg taaagttggt tttcctggaa tggattttgt attaatgcat
    57361 acaggatatc cccacggagc aaacacccct attggaataa aactcgcaca tcctgattat
    57421 tgttttttag cagacagttc tttaaaagaa aaagaagaag taattgtgtc aagcggtgaa
    57481 atcgggagaa gccttcggat aaaagtagcc gatttagaaa agatcgtcca tcctatttac
    57541 gctgatttaa ttagttaagg gacgagaaga gaagctaagc agagcagaga cctcaaactt
    57601 gagattatat ctgcttggta cttggtgcat cctgtattat ctgttacaat tgctttgtac
    57661 atactgtact atcaatccaa tgaaaatata tttagcagct atattttttc attgatgtgc
    57721 aagtgatgaa atcgttttta ataaaaaaga tacagagaaa acgcgaggtg taaaatatga
    57781 aaattacgta tcatggacat tcctgtttaa caattgaaat ggaaaatggc caacgcgtga
    57841 tgattgaccc gtttattaca ggaaatccat tgacagactt aactgcagaa gaagtaaaga
    57901 cagattggtt gctgattact catggtcata gtgaccacat aggagatatg attcctattg
    57961 caaaagaaaa tcaagccaca attatcagta ttgttgagat tgctgattat gcgaacagca
    58021 gaggagtaga ttcattcggt atgaatatcg gtggaaaaca tgcgtttcca ttcggcgtgg
    58081 taaaattcgt acatgcacag catagttcaa gctatgaagt agacggtatc gttcagtata
    58141 tgggcgagcc tagcggaatt attattcaag cagaaggaaa aacgatttat catgcagggg
    58201 atactgctta tttcagtgat ctaggattgc ttgcagaaga attcgatatt gatgtcgctt
    58261 tcttgccgat tggcgataac tatacgatgg ggccagaaga tgcagcaaag gctgcgcaaa
    58321 caattcgtgc caagaaagta gttccaatcc attacaatac tttccctgtc attgaacaag
    58381 atcccgaatc ttttgtagag ttactggaag atagtgaagg aaaaataatg agcgtctcgg
    58441 aaacgctgga agtgtaggca gaaaatggcg acaaaacatg atttgatttt agaatatatt
    58501 gaaagtttac ctgttggcaa tcgaatttct gtaagaagca tcgcaaaaga attgaatgtc
    58561 agtgaaggaa cagcgtaccg tgcaatcaaa gatgcagaaa atgtcggact agtttcaaca
    58621 attcaacgag tagggaccat ccgtatcgaa cgcaaattaa aaaaacatat tgaacgtttg
    58681 acatttggcg aggtcgtcag gatcattgaa ggggacgttt taggcggggc ggcaggatta
    58741 gacaaggtct tgaataaatt cgtcatcggc gcgatgacta aacatgccat ggaacgctat
    58801 attacgccag gatcactaat gatcgtcggc aaccgggcgg aagtccaaaa attagcattg
    58861 gaagatggcg ccgcagtatt gatcacagga ggattcaata cgactgctga gattgcaaag
    58921 ctagctgatg atcttgaaat gccgatctta cgcacctctt atgatacatt tacagtcgca
    58981 accatgatca accgagcgtt aagcgaccaa ttgatcaaaa aagatatcat gcttgtcagt
    59041 gatatctata tgccagctga aaagactcat tatttgcagc agacagatac gatttctgat
    59101 tatcagcgtt tgtcagaaga aacgcagcat tcgcgttttc cagtcgtcaa ccgccatcat
    59161 cgtttgatgg ggattgttac ggcaaaagat gttttaggga aatcgccaaa ccagatcatc
    59221 gatcgggtga tgacgaaaga accaatcagt gtaaagaaaa caatgagtat tgcctctgtt
    59281 agccatcaaa tgatctggga tgggctagaa gtcatgccgg tcgtttccga tgacttgaca
    59341 ttggaaggct tgattacccg gcaagatgta atgaaagcga tgcagcttgt ccagcgacaa
    59401 ccgcagattg cagatacgat ttcggatcag atatctggaa atatccatac gattgatact
    59461 gaccgtgaag gaaatcgttt ggaaaaccct aagttcaaat tcgtagtagc accacaaatg
    59521 gtcaatggcg ttggaaccat ttcttttggc gtacttagcg aaatcatttc tgatgtcgca
    59581 caaaaaacaa tggtgatgaa ccaaaaacga aatatcttga ttgaacaagt gaacctacat
    59641 tatttgcggc tgatccaatt ggaaagcgaa ttagacattc gtccacgtgt attggaaatc
    59701 ggcagacgtt ccgctaaact ggatatcgaa gtgtttattg aaaacaccat cgtttccaaa
    59761 gcaatcgttg tttgtcaagt aatggaacgt tcttagaaag caggagtgaa atgacagtac
    59821 agcaagaaat agtagataca ataaaaaaat acgataaaat cattatccac aggcatttgc
    59881 gccctgatcc tgatgcatta ggatcacaag taggattagc cgaactatta cgggaaagtt
    59941 tcccagaaaa agaaatacgt caagtaggtg aaacgattga agggcttcgc tttttagcag
    60001 aaatgcaaga agttgaagat gtcttctacc aaggagcgct agtcatcgtg actgatacag
    60061 ctaattcacc cagaatcagt gatcaaagat atcagttggc agaaaaactg atcaaaatcg
    60121 accatcatcc caatgatgac ccttatggag atcttgtgtg ggtaaataca aaagcaagta
    60181 gctgtagtga gatgattgca gaatttgctt tgatgtttcc tgaatcttta aagatgaacg
    60241 ttaaggctgc acggttactt tatgcaggaa tcgtcggaga tacagggcgc tttttgtatc
    60301 cctccacgac gtcccgtaca ttagagattg cttcgatatt gagaaattat ccatttgatg
    60361 caagtgcttt gaacagagag attgaacaaa tgccgatgaa agtcgctaaa ctttccggtt
    60421 atctttatca aaatatccag gtggatgaaa atggtgcagg aagaataatc cttccgcaag
    60481 aaattcttga taaatatgga atcgatgatt cagagacagc ttcgatcgtt tcacttcctg
    60541 gagtgattga cgaagtgctg gcttggggga tttttgttca gcagccttca gggtattacc
    60601 gcgtacgttt acgttctaag gggccgatca tcaatgaatt ggctaaacgc catcatggag
    60661 gaggccatcc attagcaagt ggtgcgaatg caaaagattt agcagaagta gaggaaatct
    60721 ataaagagat ccaagatata tgccaatcgt ataagagcag aaaaacagaa tgatacttcg
    60781 ttcgaaaacg tgccttttgt agtaaaaatc acaaatgtat cttttcaaga tcttcttttt
    60841 ccgttacaat agtgctcact aaagatttta aaggagaaag aagatgacat taccaaattg
    60901 cccagaatgt ggctcagagt acacatatga agatcgaggc ttgttgattt gcccagaatg
    60961 cgggaacgaa tggaacaaag aagaacaaga aactgtcata gaagacggat tagtggtatt
    61021 agatgcaaat ggaaaccgtc tgttagaggg cgatagcgtg acagtgatta aagacttgaa
    61081 agtaaaaggt gcgtcaggtg ccattaaaca aggaacaaaa gtcaagaaca tccgtttagt
    61141 agaaggcgat cataatatcg attgcaaggt agaaggattt ggaccgatga aactgaaatc
    61201 ggaatttgtg aaaaagaatt aaagaatatt caaggtcttt tcacattctt ttcctactac
    61261 taatgataaa ataaagtaaa cgaaaaggag tgaaacgaaa atgtatggtt atggaatgta
    61321 tggtttttta gatccgacat ttcttttggt cattcttgga ttagttattt ccggaattgc
    61381 gtcagcgtac gtcaatagta ctttccgaaa atacgatcaa gtgagaagca aaaacaatgt
    61441 gactggtaca caagcagcac aatatatatt acaaagtcaa cagataaata atgtcggtgt
    61501 tcaacaaatt gccggagatc tcacagataa ctacaattca ggtaataaga tgttgagttt
    61561 gtctcaagca actgcacagt ctacttcagt tgctgcgata ggtgtagcag ctcatgaatg
    61621 cggacatgcg gtacaagatg cttcgggtta tgtcccattg aagattcgtg cagcacttgt
    61681 tccagcagct aactttggtt cgatgatttc gttccccttg attatggtag gtgtattgct
    61741 tagctggaac tctacgttaa tcaatatcgg gattttcgct ttttctttag cattactttt
    61801 ccaattggtg acattaccag tcgaatttaa cgcgtctcgg cgagcaatcc aaattttgag
    61861 tgaaggcgga ctactgacgg aagaagaagt accgatggca agacacgtat tatttgcagc
    61921 agcactgact tatgttgcag cagcgctttc gacattcctg cagctgttgc gcttgatctt
    61981 attgttcggc ggcaaccgaa gagattaaaa taaagaagtt gtgaaatcag cgctttgatc
    62041 tgagctaata ccgagagtta gctgattgaa taccatttat ttggaa
//
LOCUS       DAZKOU010000005        59314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000005 DAZKOU010000000
VERSION     DAZKOU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.005"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG827_000256"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG827_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0482431.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG827_000257"
     CDS             852..1040
                     /locus_tag="ACG827_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0482432.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG827_000258"
     CDS             1053..1610
                     /locus_tag="ACG827_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0482433.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG827_000259"
     CDS             1642..1884
                     /locus_tag="ACG827_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0482434.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(2185..3264)
                     /locus_tag="ACG827_000260"
     CDS             complement(2185..3264)
                     /locus_tag="ACG827_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0482435.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            3614..4828
                     /locus_tag="ACG827_000261"
     CDS             3614..4828
                     /locus_tag="ACG827_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0482436.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            4859..6286
                     /locus_tag="ACG827_000262"
     CDS             4859..6286
                     /locus_tag="ACG827_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0482437.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            6309..7235
                     /gene="glsA"
                     /locus_tag="ACG827_000263"
     CDS             6309..7235
                     /gene="glsA"
                     /locus_tag="ACG827_000263"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0482438.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7443..8903
                     /locus_tag="ACG827_000264"
     CDS             7443..8903
                     /locus_tag="ACG827_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482439.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9124..10887
                     /locus_tag="ACG827_000265"
     CDS             9124..10887
                     /locus_tag="ACG827_000265"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0482440.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            10971..11429
                     /locus_tag="ACG827_000266"
     CDS             10971..11429
                     /locus_tag="ACG827_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0482441.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11538..12221
                     /locus_tag="ACG827_000267"
     CDS             11538..12221
                     /locus_tag="ACG827_000267"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0482442.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12368..12982
                     /locus_tag="ACG827_000268"
     CDS             12368..12982
                     /locus_tag="ACG827_000268"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0482443.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            12998..13204
                     /locus_tag="ACG827_000269"
     CDS             12998..13204
                     /locus_tag="ACG827_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482444.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     regulatory      13429..13595
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            13743..14300
                     /locus_tag="ACG827_000270"
                     /pseudo
     CDS             13743..14300
                     /locus_tag="ACG827_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            14358..16400
                     /locus_tag="ACG827_000271"
     CDS             14358..16400
                     /locus_tag="ACG827_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0482445.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADDKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAE
                     IKEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(16509..17660)
                     /locus_tag="ACG827_000272"
     CDS             complement(16509..17660)
                     /locus_tag="ACG827_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0482446.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(17814..18344)
                     /locus_tag="ACG827_000273"
     CDS             complement(17814..18344)
                     /locus_tag="ACG827_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0482447.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(18443..18793)
                     /locus_tag="ACG827_000274"
     CDS             complement(18443..18793)
                     /locus_tag="ACG827_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0482448.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(18950..20479)
                     /gene="ahpF"
                     /locus_tag="ACG827_000275"
     CDS             complement(18950..20479)
                     /gene="ahpF"
                     /locus_tag="ACG827_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0482449.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(20489..21052)
                     /gene="ahpC"
                     /locus_tag="ACG827_000276"
     CDS             complement(20489..21052)
                     /gene="ahpC"
                     /locus_tag="ACG827_000276"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0482450.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(21196..21858)
                     /locus_tag="ACG827_000277"
     CDS             complement(21196..21858)
                     /locus_tag="ACG827_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0482451.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            22042..23688
                     /locus_tag="ACG827_000278"
     CDS             22042..23688
                     /locus_tag="ACG827_000278"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0482452.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            23679..24761
                     /gene="uxuA"
                     /locus_tag="ACG827_000279"
     CDS             23679..24761
                     /gene="uxuA"
                     /locus_tag="ACG827_000279"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0482453.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(24869..26422)
                     /locus_tag="ACG827_000280"
                     /pseudo
     CDS             complement(24869..26422)
                     /locus_tag="ACG827_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            26570..27711
                     /locus_tag="ACG827_000281"
                     /pseudo
     CDS             26570..27711
                     /locus_tag="ACG827_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(27837..28916)
                     /locus_tag="ACG827_000282"
     CDS             complement(27837..28916)
                     /locus_tag="ACG827_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0482454.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(29025..29465)
                     /locus_tag="ACG827_000283"
     CDS             complement(29025..29465)
                     /locus_tag="ACG827_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0482455.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(29522..29932)
                     /locus_tag="ACG827_000284"
     CDS             complement(29522..29932)
                     /locus_tag="ACG827_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0482456.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(30113..30547)
                     /locus_tag="ACG827_000285"
     CDS             complement(30113..30547)
                     /locus_tag="ACG827_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0482457.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(30647..31495)
                     /locus_tag="ACG827_000286"
     CDS             complement(30647..31495)
                     /locus_tag="ACG827_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0482458.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            31884..33650
                     /locus_tag="ACG827_000287"
     CDS             31884..33650
                     /locus_tag="ACG827_000287"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0482459.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            33752..35011
                     /locus_tag="ACG827_000288"
     CDS             33752..35011
                     /locus_tag="ACG827_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0482460.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            35158..36459
                     /locus_tag="ACG827_000289"
     CDS             35158..36459
                     /locus_tag="ACG827_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0482461.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            36462..37313
                     /locus_tag="ACG827_000290"
     CDS             36462..37313
                     /locus_tag="ACG827_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0482462.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            37326..38141
                     /locus_tag="ACG827_000291"
     CDS             37326..38141
                     /locus_tag="ACG827_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0482463.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            38292..39458
                     /locus_tag="ACG827_000292"
     CDS             38292..39458
                     /locus_tag="ACG827_000292"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0482464.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            39688..40335
                     /locus_tag="ACG827_000293"
     CDS             39688..40335
                     /locus_tag="ACG827_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0482465.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            40419..41363
                     /locus_tag="ACG827_000294"
     CDS             40419..41363
                     /locus_tag="ACG827_000294"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0482466.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            41498..42949
                     /locus_tag="ACG827_000295"
     CDS             41498..42949
                     /locus_tag="ACG827_000295"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0482467.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            42970..43806
                     /locus_tag="ACG827_000296"
     CDS             42970..43806
                     /locus_tag="ACG827_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0482468.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            43806..44465
                     /locus_tag="ACG827_000297"
     CDS             43806..44465
                     /locus_tag="ACG827_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0482469.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            44591..45280
                     /locus_tag="ACG827_000298"
     CDS             44591..45280
                     /locus_tag="ACG827_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482470.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            45286..46242
                     /locus_tag="ACG827_000299"
     CDS             45286..46242
                     /locus_tag="ACG827_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0482471.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            46286..46798
                     /locus_tag="ACG827_000300"
     CDS             46286..46798
                     /locus_tag="ACG827_000300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0482472.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(47054..47602)
                     /locus_tag="ACG827_000301"
     CDS             complement(47054..47602)
                     /locus_tag="ACG827_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0482473.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(47691..48872)
                     /locus_tag="ACG827_000302"
     CDS             complement(47691..48872)
                     /locus_tag="ACG827_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0482474.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(49108..50616)
                     /locus_tag="ACG827_000303"
     CDS             complement(49108..50616)
                     /locus_tag="ACG827_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0482475.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            50775..50966
                     /locus_tag="ACG827_000304"
     CDS             50775..50966
                     /locus_tag="ACG827_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482476.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            51169..52086
                     /locus_tag="ACG827_000305"
     CDS             51169..52086
                     /locus_tag="ACG827_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0482477.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            52079..53152
                     /locus_tag="ACG827_000306"
     CDS             52079..53152
                     /locus_tag="ACG827_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482478.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            53300..53818
                     /locus_tag="ACG827_000307"
     CDS             53300..53818
                     /locus_tag="ACG827_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482479.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            53799..54191
                     /locus_tag="ACG827_000308"
     CDS             53799..54191
                     /locus_tag="ACG827_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0482480.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            54267..55202
                     /locus_tag="ACG827_000309"
     CDS             54267..55202
                     /locus_tag="ACG827_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0482481.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            55335..56114
                     /locus_tag="ACG827_000310"
     CDS             55335..56114
                     /locus_tag="ACG827_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0482482.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            56107..56424
                     /locus_tag="ACG827_000311"
     CDS             56107..56424
                     /locus_tag="ACG827_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0482483.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            56668..58278
                     /locus_tag="ACG827_000312"
     CDS             56668..58278
                     /locus_tag="ACG827_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0482484.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            58435..59283
                     /locus_tag="ACG827_000313"
     CDS             58435..59283
                     /locus_tag="ACG827_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0482485.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        19541 a        10607 c        11669 g        17497 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttacgtaaa tttcaagttt aagttagcca cccatggtga cccggttgct
     2041 cgttaccacc aaccgtctaa tttttctaat cgagtgggcc cttgacaatg agctgaggca
     2101 tggtagtctc ccgactggct ggccccggcc agctttccag acacaagcca tgccgaggga
     2161 ggctcgttgt caagggccca cgtgtcaagc cgtcaaccgt tcctgcctaa atgcctggac
     2221 gaacgtgtca tgcggcgttg catagcccaa aatctttcgc gggtaatcgt tcatccaacg
     2281 ctgaatccgc aagatttggt tttcgctcac gttgccgatt gggctgccct tggggatgaa
     2341 acggcgaatc agcttgtgtt ggttttcgct cgtcccgcgc tcccaggaag cataggggtg
     2401 gctgaagtag atgtttgcca catcctgcaa ggcatcgtgc agtcccgcga actccgagcc
     2461 gttgtccgag gtaaccgtct tgaacagctg gccgaagtac gcaccagccc gttctctcag
     2521 ctgtcggacc gcttggttga ccgattcggc atccttcccg gccagcttca gaatgacctc
     2581 gaagcgtgtc tgccgttcca ccaacgtcag cagcacggca tccgacttgg tcttgtttcc
     2641 tacgaccgtg tcgacttccc aatggccgaa ggttttccgg gtaccaaccg cctccgggcg
     2701 ctcttcaatg gatttcccta gcacccgttt attcgggcgg ttgccatgac gggacgattt
     2761 cggtttgcgg gacagttttt ctagcaggtc catattcttc gttttcatga tgccacggtc
     2821 gatccactga tagagggtgg tggtcccagg gataatggcc gggtcgaaca attcgtgttt
     2881 tctggcaaag cccaccacca cgtccgggga ccatttttcc aacagcatca tgtcgtccgc
     2941 ccattcaatg aaggcgtccg tgtcggccca taagggccgt ctgccgcagt tcaagcggtt
     3001 cttctcgtag gccgcctgcc cggcatcggc gtcgtagacg tcatgataat agtcgtagac
     3061 cttcccgttc tgattctggc ggttaagctg agtaacggtc ccgcgtttga tttcgttgtt
     3121 tatggtctgc ggcacgcagc ccaggacatc cgcaacctga cggttggaat agttttcctt
     3181 tttcaggatg gcgatttggc aacgttctga gtaggttagg tgttttccct tgcgtgaaac
     3241 tgtgttatca tggttttgcg tcatgtgaat tcatccttgt ctattgagag ttgtggtaac
     3301 ttcaatataa catggaattc acgtggcgtt tttttatttc caaatggcta agttaattat
     3361 aaaatccgcc tctttattcc ttatgaaaaa atatctttaa aaataaaaaa agacgaaaat
     3421 aaacggtttt ctataaaaag aagaccgttt attttttgtc tttatttgtg tgaaaaacga
     3481 aaaagagcac aacacatcaa gcttttatta attgtctgtc ttttataaat gcaagtatta
     3541 attcaaaaaa acagaagaaa aaaacaacta caccttcttt tatactcgta atcgtagaaa
     3601 ggaggcgaaa aaaatgaata cattacctat caatacagga gatacagcat tcatgatttt
     3661 atgtacagca atggtttgcc tgatgactcc tggtctagct tttttttacg gcggattagc
     3721 acggaagaat aatatactaa cgatcatgtc tcaatctttt ctctccgttg ggataacaac
     3781 gattatgtgg gtatttggtg gattcggatt ggcctttgga cgggatatcg gcggtgttat
     3841 tggaaacccg gcagatttct ttttgatgag acatgttaca agcttcccca acatgtttca
     3901 tggtgcaacg attccatttt taatgttctt tctttaccaa ctgatgttta tcgtgattac
     3961 cgttcctttg atgacaggag cttttgcagg gagattgaat ttaaaaggct atattatact
     4021 tgttattttt tggaatctgc tgatttattt ccctgtttgc cactggatat ggggtggcgg
     4081 atttttggat cagatgggat ttagagattt cgctggtgga gcagtcatcc acacgacagc
     4141 tggattcggc tctttagcat gtatcctcac acttggtcaa agaaagattc ctttaggaga
     4201 aagaagtcag catagcagtc ttatggcagc tgctatcggt acaggactgc tttggtttgg
     4261 ctggttcggt tttaattccg gtggtgcact tcgagcagat gaacaagcag tcaatgcatt
     4321 tggttcaaca ttcatcgctc ttgcatttgc gatgattact tggctgatca ttgcaaaagt
     4381 aaaaggaaat ggctttgatt ttgtcgacgt actaacagga tccgttgctg gattggcagc
     4441 cattacgcct tgtgccggtt atgtagaagc aaaaagtgcc atgctgattg ggatcattgc
     4501 gggtatcgtc tgtcatgctg ctgttgattt tcgtaaaaag aaacaatggg atgatgcact
     4561 tgatgtgtgg ggtgttcatg gcatgggcgg attcacaggt acgattctga tagggatctt
     4621 tgcatctaac agccatttac taacgaatcc aagcagctgg tacttcttag ggattcaagt
     4681 gattggtgtc atcattactg ccgtgtatgc ctatgtgctg acaacagtga ttttaaaatc
     4741 ggcgatgcac tttacaacga tcactactac aaaagaagaa caagaagaag gattggactt
     4801 tttcttcttt ggcaacaaac tcaattgaaa caaattcaaa tagaaaagag gaagattcat
     4861 gcaacaaagc aaaacgaaaa ttcctgcaaa aaaattaacg ttctttggct ttttggctat
     4921 gaccatctca atggtcgttt cattatatga atatcccaca ttcgcgacct caggcttcag
     4981 cttagtattt ttcttgttat taggcggttt gctatggttt atccctgtgg cattgtgcgc
     5041 agcagagatg gcaacggtga aaggatggga aaaaggcggt gtctatactt gggtcagtcg
     5101 cacattggga aaacgattcg gttttgccgc aatctttttc caatggtttg agataacagt
     5161 tggctacttg accatgctgt atttcttaac tggtgcatta tcctatgcaa cgggtattgg
     5221 tgctatacaa aacaataaat atctgaaatt ggcaatccta ttgattattt tctgggctat
     5281 tttactctca cagttaaaag gaaccaaata cacttcattg attgctcgga tcggttttgt
     5341 agctggtatc ttgttgccag cattagtctt gtttggctta gggatccatt atgtcgcttc
     5401 aggcgctcca ctacaaacga tactgtctat gaaaacattg attcctgatt tttcgaaatt
     5461 gcctacgctg gttgtttttg tttcctttat tttagcatat atgggcgtag aaacatctgc
     5521 cagtcatgca aatgaaatgg aaaaccctaa aaaggattat ccattagcaa tgtttgtatt
     5581 ggttattctg gcaatcattt tagatacttt tggcggattg accgtcgcta ctacagtacc
     5641 tcaacatggt ttgtctctta atacaggggt gattcaatcg ttagaattcc tattaagaca
     5701 cattgatcca aatcttgcat ggattgcgaa aattcttgcg attcttgttt gtttaggtgt
     5761 catcggagaa attgcttctt gggtaacctc tccttcaaaa gcacttcacg tagcagcaga
     5821 agaaggatta cttcctgaat attttgcaaa agaaaacaca cacggtgttc ctgctcattt
     5881 gatgatcgca aatgggatcg ttgcaacaat ctgggcagcc gtctttacat taagcggcgg
     5941 ggggaataat ctttcatttc ttgcagcgat gtcattgact gttgtaatct acctgatgtt
     6001 gtactttatg tttttcgcgg gctatttgaa gctggttttt aaagatgaca gtcttgaaag
     6061 agcctatcag gttccaggcg gaaaagttgt aaaagtaatc gttgcttgtt taggtctttt
     6121 gacttcactt tttgcttttt gtatctcatt tgttccacct gctagtatcg cagctggaca
     6181 gcatgggatt tatgaaacga ttctgattgt ttcttatctt atcgtcttag ttattccatt
     6241 tacgatctac gcatgccgtc ataaattcca gcatgcagaa aaataaaaaa tgaaagctgg
     6301 ttattcaaat ggaagtaaaa gaattagaac aattgattgg aaaaaatttc cactattatc
     6361 aaacaggaca agtggcggat tacattccag cattagctga agtggatccc aaacaattag
     6421 gtatggcgat ttatgatttg gatacaaaac aactgattga gggaggagac gccgatgtgc
     6481 gctttgccat cgaaagcatg tcaaaagttc ccgtattgtt attagcaatc aaagacaacg
     6541 gaatagaaaa agtctttcaa acaatcaaca cggaaccaac cggttttgcc ttcaattcta
     6601 tcatgaatat ggaaatcaat catcgcaaac atccaatgaa tccttttgtt aatgcaggag
     6661 cgattgcaac aacctcgttg attgccggta aagatgcaga tgaaaaattt gagcgaatct
     6721 tgtcatttat gaaagagatc tgtgatgatc ctaatatcac attgaatgaa gaaatctatc
     6781 attctgaatc aagaacaggt gatatcaatc gctctcttgc ttactatatg aaaggtaatc
     6841 aaatgatcga aggagacgta ccggagatac tcgatgctta tttcagacaa tgctccctca
     6901 atgtcaccgc tacaggcata gcaaaattag ctgctgtgct ggcaaataaa ggaattgctc
     6961 cttggaatgg caaacaaatc attactgaag aaagtgctac aatcgtcaaa tcaattatga
     7021 caactgctgg tttgtatgat gaatcaggag agttttccgt ccatgttggt gtacctgcaa
     7081 aaagcggtgt aggcggcggg ttgatggcag ctgtgccaaa tcgttacggc ataggtgtct
     7141 tcagcccggc actagatcca tttggtaaca gcgcagctgg tatccaattg ttgaaagatg
     7201 tagtcaaaga actagacgct gacatctttg aataaccatt cttgaaagag gctggggtaa
     7261 aagtccaaac ggctcaaagc tacctacaat gaggctgaaa ttgtgagctt gtttgacttt
     7321 tgtcccagtt tcttttttta ttggtttttg aaagggcttt tctaaataaa aaggaaacaa
     7381 aaatacgatt tacagatatg attaaataaa atcataagaa aaactggagg cgtgtatcag
     7441 gaatgctgga aaattacatc gagcgaaata tatttagaaa agtgtatctg tgtgaacaat
     7501 tatttgaatt ccaagaaata gacatcgaac aaacagctat ttctctaagg gtgactacac
     7561 caacgatcct tcacgatcta gaaagtctag ccgagtgtct agaatattgt ataaaagaac
     7621 aagtaagaga gaagcataag tataaactcg tattcaaaca tggtattgct ctttctgagc
     7681 tcacacagtt tctgtacggt caatcttatt ttttaaagtt tttaagttat tattttaatg
     7741 gtatattcac gagtacagaa ctggctgatc ttgaatttat ttctttgagc aaagtatata
     7801 caatcaaaaa aatcgtattg gattttttta aagaaaatga ttatttgaaa aataaagaaa
     7861 tcattatccc agaatttgac tcgcggaaca tcttgctagc tttagtacgt tatgtcaatt
     7921 gggaaggcta tgaaaataaa aatccgtatg tagaagaagc ttgtaacgag ttgattgatt
     7981 atgtagagac tcattttttt aaaagaagat attcggaaga tgaaaagtcc tttatcatac
     8041 gaggaataga gatcgcaatc ggaagaaagg catttcccct tggactgaaa gaagaagata
     8101 agaaaagcgc agaaacgaag ccgttatttc aattagtctg tcaaggattg gaagagcaaa
     8161 aaggaattct agacttacaa gaagaagatg tctattatat tttttctttg ttcaacagta
     8221 gaaattatac caatgagaac atggagctgt taagaaaaga tttcgaagtt gtttataaaa
     8281 attttgttct caatgaccta tcactgaatg aattgatttt acagattatt agtatcgctg
     8341 aaatcgacga tgcagaaaat tttattttca agcaatcctt tttacaattt gtccgtacgt
     8401 tgtgggcaga tggacaagta tttttaccgg aaaggatcta tcttctatca gaaaaagaac
     8461 aacttctgta cggaaaaatt gtagaaatat tggaagagtg gaaaaagaaa aatcaaatcc
     8521 aactaagatg gaatgaaaat ttcattcgaa aattcgtcaa aagtctttct ttgatcaaca
     8581 gtgaacaagc ggggagacag gaaatagaaa tttttgttgt ttcagaaagt tcagtcaaac
     8641 aattttttta tcgaacccaa tttcatttgt atgcggaaga aggcatcgct gaaataaatc
     8701 ctattttatt tcgttcatta gaagaattgc ctgatgaatg tctatgtgcg aaaaaaagag
     8761 tggttttgtg cgatgctgct agctatcaaa atggattaga aactgaaaag acaaagatct
     8821 atccaatctc attaaaaggt gcaaacatcc atttgtatca tattgctcag gaacttcacc
     8881 tattaaaaaa acatatttct tgatgagacc ttaattagga gaccttctac aaagcttttt
     8941 aattgtctgt agaaggtttt tatttgagtc ctgattcgtg cgaagatcta cttatataac
     9001 aagctttctc tgaaattttt ttaaaatgct attgacttag agtcaactct aagtagtaga
     9061 atagataaag tgtaacaaat ctattttcat taaaagaaga tagtacggag gagagaatgt
     9121 attatgagta cagtattaca tgtattggat gcaatcaata gccccgcaga tgtcaaaaaa
     9181 ttatctgttg aggaaatgaa acagttggca gaagagatgc gccaaaaaat cttgcaaaag
     9241 gacagcgatg taggtgggca cgttggtccg aatctaggtg tagtagaatt aacgattgcg
     9301 tttcattatg tatttgattc accaaaggat aaaatcgttt gggatgtttc ccatcagtct
     9361 tatggtcata agcttttaac tggtcgaaaa aatggttttg aaaaaggaca ttaccgtgaa
     9421 gtgagcggat attccaacca acatgaaagt aaacatgact attttacagt ggggcacaca
     9481 tctacatcta tcagcttagc aacaggttta gctaaagcga gagatctgaa gaaagaatca
     9541 ggaaatgtgg tagcatttat cggtgacggc tccctaagtg gtggcttagc ttttgaagga
     9601 ttgaacaatg gtggtgtttt acaatccaat ctaatcgtat tagtaaacga taatgagatg
     9661 tctattgatg agaatcatgg cagtttatat aaaaacttag ctgagcttcg tcaaaccaac
     9721 ggtaaaagca gcaataacct atttaaagca atgggatttg attatcgtta cgtggataat
     9781 ggaaacgatc tggaaacggt gatccgttta tttgaagaag taaaagatat tgatcatccg
     9841 attgtacttc acgtccatac ggaaaaaggt cgcggttatc agccggcaat cgataataaa
     9901 atgaaatatc attggcatgt accatttgat ctgtccaccg gggaggcaaa gcaagtaact
     9961 tctggagaaa catttaatat gatcatcatg gattatttgg ataagaaaat gcaaacaagt
    10021 acagctccta ttgccgcagt caatgcagcg ataccaggcg cctttggact gaaaacattc
    10081 caagaaaaac atccagatca ttattttgac gtggggatcg ctgaacagca ctctatcact
    10141 ttcgcttctg ctttggcttc tcagggagta cgtccagtcg tcttccaatc aagtactttc
    10201 ttgcaacggg catatgatca attatcacat gatttagcga tcaacgagaa tccagtgatt
    10261 attttagtcg gtggcggaac gatctcagct agtgaccgaa cacaccaagg aacatttgac
    10321 attccttact tatcaagtat cccgaacatc aaatatcttg ctccagcaac aaaagaggat
    10381 atgatctcta tgctagattg ggcgttcgaa caaacagcgc agcctgtagc aatccgaatt
    10441 cctacacatg gtgtagaaca tggtacagct atcgcaaatg attatagtat tccagcctat
    10501 caaacagtaa ataatggagg aaaagtagca attttagctt taggcggttt tcttgaatta
    10561 ggaaaagaaa cagtaaaagc ttttagaaaa aaaggaatcg aagcaacgtt gatcaatcca
    10621 ggaacgatca cagaaaatga cgtagaaaca ttagataaat taaaagaaac acacgaatta
    10681 gtcatcacat tagaagatgg tagtttgtca ggaggatttg gcgaaaatat cagtcgttat
    10741 tatggtgatt cttcaatgaa cgtattaaat tttggtgcag agaaagaatt cacggatagc
    10801 gttcctgtag ggcagcttta tgaacgttat catttaacac caaaacaaat cgttgaagat
    10861 agtctgcgtg tcttgaatat tcaataattc attatgctca ccatccagat aattgtttat
    10921 actagtatct ggatggtttt ttcattataa aaagcaaagg ggtggatgtt atgtatcagc
    10981 aagtagaaga atatgtcaaa aagttccaag aaaaatatcc tagttatcaa tcagaaaaat
    11041 taggtttctt ttcatttggg gatacagaaa aaatggcaga cgagttgagc gaactagttg
    11101 taaaaaagat aaagactggt acatcaagtg gttacgatct gtataaacaa gagaatgagc
    11161 ctttaccaaa agttggtcaa ttagacgtgg tcctgaatgg aaaaaatgaa ccagtttgta
    11221 tcatcaaaaa tataaatgtg accatcttgc cttttagtga agtcacgaaa gagcatgctt
    11281 ggaaagaagg agaaggggat cggacattag cttattggcg gaaagtccat gctgagtttt
    11341 ttaaacatgc ttataaagaa gcgttgaaca tagaatttac agaagagaag ctggtcgtat
    11401 acgaagaatt tgaagtaatt ttcttttagt aagaaaggtc tgctgaaaga aatagctcat
    11461 tattgtctcc tgaatagaaa agggttacat ttttatatga agctgtattt ttatataaca
    11521 ttactggagg agatattgtg gatacactgg aacaaatcaa aggcggagtc gtcgtttctt
    11581 gtcaagcttt ggaaaacgag ccgctacaca gttcttttat tatggggaga atggctttgg
    11641 ctgcaaaaga aggcggagca gttggtattc gagcaaatac gacagaagat atcaatgaaa
    11701 tcaagcgaat cgttgattta ccggtaatag ggattatcaa aagagattac gatgggtcag
    11761 aagtgtttat tactgcgaca gaaaaagaag tggaagaact tttggagacg caagcagaaa
    11821 tcattgcact ggatgcaact tctcgtaaac gtccgacaga aaaaacaact gctgaacttg
    11881 tccaaatgat tcacgaacac ggtcggttag ctatggcaga tatttcgact tttgaggaag
    11941 cgcttcaagc gcaagaagat ggttttgatc ttttgtctac gactttagca ggctatacag
    12001 aatattcacg aaagacgcag acacctgatt ttgatttatt gaatcaaatc atagaacaag
    12061 tcgatatccc agtaattatg gaaggacata ctgactctcc tgaacaagta gagaaagcat
    12121 atgaattagg tgctttttca gtcgtagttg gatctattat cacccgtccg caattgatca
    12181 ctaaaagata tgtcgaagcc gcaagtaaag caaataacta aaaaacacgc acggcacaga
    12241 ttcttgacaa aaaagaaaaa ttgatagata gtttaaagca gattctaaga agatgaaaaa
    12301 agcattactt ttcattttgt cttcaagatc tgctttttaa cgaattttat agaaagaagg
    12361 cgaaaccatg tttgaacgga gtccttcaga attgacagaa cgtgaaaatt acaagttgct
    12421 gattggttca gtcatcccta gaccagtagc agtagtaacg actaaatcag caaaagatat
    12481 agtgaatatc gcacccttca gttacttcaa tatcgtcagt tcgaatcgac ccatcttatc
    12541 cttggcgatc cagagaaaac aagatgcagt aaaagacaca gcaaagaata ttcttgagac
    12601 aagagaagct gttatccata tccttgatga ggacaacgta gaagaagtaa ataaaacagc
    12661 agccaatctg cctaatgagg aaagcgaact gtacctgact gacttgactt taactgattc
    12721 agtaatcatt tcagtaccag cattgacaga agcaaaagtt cgttttgaaa catccttata
    12781 tgaacatgtc gagatacgga ataaaaataa agtaacagct gatttatttt tgctagaagt
    12841 acagaaatat catttaaatg aaacagtcta tgatgataag acaggaagga tcgatccaag
    12901 aaaattgaat gctgtaagcc gtttggcagg aaatgattat gcagcaatag gagaaatttt
    12961 cacgattgca cgtcccgaat aaaaaggagc actagttatg ttaaaaataa gctataaacc
    13021 atcgacagat tcaaaagaaa tgaaaaaaga atatgaaaca gtgaatgatt ttctgcaagg
    13081 gcagtactta gaagttccgc cattgcaaga ccattttgtc gttactactg ttactttaga
    13141 tggtaaggaa atagaaatgc cagatcaaac gattagcgga ttgtttaatt actttaacaa
    13201 ataaatattt attccgaaaa gtgaaaaaac atataaaaaa acacataaaa ctaaaaaatt
    13261 atcaaagaat cctggtgcaa ctgaaagaat ttgcaacggg attctttcaa cttatttgtt
    13321 atttttttct aaaaaatatt aaagattgtt aaatttcact atttcatttc aaattccatt
    13381 gcctaatcat taggaaaagt ctatcatgaa ctcgtaaata gaatatagta ataaccttgt
    13441 taggtgaggc tcctatacaa acataggctg ttgcccagaa acgtcgaaag tcgccaatgg
    13501 gtaaaacaga aactgttgga ttaaggcttt ttccaatagc taagaatgtt ttctttacgt
    13561 tgtatagtgc taaaactcgg acgatgagat gatgctaggt cagaattctt gttcgaaaag
    13621 gtttctgacc ttttctttat atttttgtga ctgacttatt tgatgagaca aactcgctcc
    13681 tcacctgaca agtggattgc tgtgtgagga ggagatgcgg tgaaaaaaga tcgattggtg
    13741 gaggtcgctc ggattcactt ctggttgcac aatacaacca gaaaacacaa caagcaaaac
    13801 tgatctctat catgagagat tcatatgtcg atattccagg ttacggaatg aataagataa
    13861 atgcagctta ctcttatggg ggagttgatt tattaaatca aacattaaag gaaaatttca
    13921 aatttgaagc tccctattat gcaagtatca catttcaaga ttttatcgat tgcgttaatg
    13981 aactgtttcc tggtggagtc aagatcgatg cagaaaaatc tctagattta gatggcgtat
    14041 atataaacaa aggagagcaa gtgatggatg gtaatacttt actccagtat gctcgatttc
    14101 gggaagacaa agaaggagac ttcgggagga tcagaaggca gcagcaagtg atcaaagcta
    14161 tctctcagca gctgaaagac gtaacttcaa tattcaagct acccaaagcg gttgggaaat
    14221 tacttggtag tatccaaacg aaccttccag aaagtgtgct tctggattgt ggtatggatt
    14281 tcctcaaaga catatgttag aataaacagg tataaatagt gaaataaagg aatgacaagc
    14341 aagagaagga ggaaaaaatg aaaagaagtg acaagcacgg caaaaatcga acaggcgctt
    14401 atattgccgg cgcagtgatc ttaatagcag ctgcgggtgg cggttatttt tactaccagc
    14461 actatcaaga aacccaagca gtagaagctg gagaaaagac agttgagcaa tttgtccaag
    14521 ctttgaacaa gggagattat aacaaagctg cagaaatgac atcaaaaaag gcggcaaata
    14581 aaagtgcatt atctgaaaaa gagatcttag ataaatacca gaatatatac ggtgctgctg
    14641 atgtcaaagg acttcagata tcgaatctaa aagtagataa aaaagacgat tctacttaca
    14701 gtttttcata taaagcaaag atgaatacct cattaggtga attgaaagat ctttcttata
    14761 aaggaacatt agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt
    14821 ttccagaaat ggaaggaaat gacaaagtaa gcctgaccac acaagaagca gcaagaggga
    14881 atattataga tcgaaatggt gaaccattag caacaacagg caaactaaaa caattaggag
    14941 tcgttccaag taaacttgga gatggaggcg aaaaaacagc caatatcaaa gcgattgctt
    15001 cctccttcga cttaacagaa gatgctatca atcaggcgat ttcacaaagc tgggtacaac
    15061 ccgattactt tgtcccattg aaaatcattg atggagcaac gccagaactt ccagctggag
    15121 ctaccattca agaagtagac ggcagatatt atcctttggg tgaagcagct gctcagctga
    15181 ttggttacgt gggagatatc acagcagaag atattgataa gaatccagaa ttaagcagta
    15241 atggaaaaat cggtcgctca ggtttggaga tggcttttga taaggatctt cgtgggacaa
    15301 caggtgggaa attaagcatt acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac
    15361 atgaagttca aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca caaaaaatag
    15421 cttttgacag tctaggagga aaagctgcat caactgttgc gacaacgcca aaaaccggtg
    15481 atcttcttgc gcttgctagc tctccaagct atgatccaaa caaaatgaca aacgggatct
    15541 cacaagaaga ttacaaagct tatgaagaaa atcctgaaca tccattcatc agccgatttg
    15601 cgacaggtta tgctcctgga tctacgttta aaatgattac agcagcaatc ggtctcgaca
    15661 acggcactat cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca
    15721 gttcttgggg atcttttaaa acaacgcgtg tcagtagtga tgtatcacaa gtagacttaa
    15781 aaactgcttt gatctattcc gataatatat atgcggcaca agaaacgttg aaaatgggtg
    15841 agaaaaaatt tcgtacaggc ttagataaat tcatttttgg tgaagacctt gatttgccaa
    15901 ttagtatgaa tccagcacaa atttctaatg aagatagctt taactcagat atcttgctag
    15961 ctgatactgg atatggacag ggcgaacttc taattactcc tatccagcaa gcagcaatgt
    16021 attctgtttt taccaacaat ggcacacttg tctatcctaa attgattgca gatgataaag
    16081 agacaaaaga taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc
    16141 tgagagaagt tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc
    16201 cgttggcagc gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag
    16261 agaacagttt cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt
    16321 tggaaaataa agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc
    16381 tcaaccaaaa ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta
    16441 tcgtcacagt ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt
    16501 tgatttactc acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata
    16561 agtgtactta ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga
    16621 ctttgctgtc caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca
    16681 ccgaccatca atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca
    16741 agcaatttgg tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta
    16801 aagtcagaaa aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca
    16861 tatcctaaag cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct
    16921 atcccagcaa aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac
    16981 attcctaaac aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct
    17041 gcaaatgaaa gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa
    17101 attagctgtt caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga
    17161 aaagataggc tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc
    17221 aaaattgttg atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa
    17281 atactgacag aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact
    17341 tggaatgctt tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca
    17401 acgaacatac caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct
    17461 aaaaacatta ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg
    17521 acccaattca atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct
    17581 gggataccta atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat
    17641 aaaataccaa taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc
    17701 ataacataca ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa
    17761 gatttttttg gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt
    17821 atagtcattt ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa
    17881 ataaagccga atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg
    17941 aatccgccaa tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc
    18001 atgctgaaga acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc
    18061 aggatagaaa cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa
    18121 agaccactga acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca
    18181 aaaagattca acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga
    18241 aaaacagcag aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt
    18301 aataaggcgt atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt
    18361 tgacttattt taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag
    18421 gagaagaata tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg
    18481 ctagctcatc gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt
    18541 cttctactcg tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag
    18601 gggcttccgc aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg
    18661 tttgggaata acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt
    18721 ttctctccgc ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac
    18781 gattttgcgc cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata
    18841 taagaaaaag agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc
    18901 ttgtcaccat ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca
    18961 tcagataatc gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt
    19021 gtttgtatgg actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc
    19081 catttttctc ggtcactatt tcatttcttg gattcatttc gatcgttcta tctagccaat
    19141 ccgtattagg gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct
    19201 ccatcgtacg gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt
    19261 tcgtcgctgc atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga
    19321 tttgatctgc ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag
    19381 ccaaatcgat tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc
    19441 ctttaaataa agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt
    19501 tttcgcctgg tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg
    19561 ctttaagtga tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg
    19621 aaacggcctg ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt
    19681 gctgcatcaa ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg
    19741 tttcaacgac ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg
    19801 ctgcatagat agctgcacta ctagctgctg gtccaccgcc aatcactaat acatcataag
    19861 gttctttttc aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt
    19921 cgatcgtcat gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca
    19981 tgattttttt atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa
    20041 tatttggatt taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac
    20101 aagtcaagct aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac
    20161 tcaaagattc actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag
    20221 aggaaaattc atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg
    20281 gattgataga aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag
    20341 acatttctgc tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca
    20401 aactaacaga aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt
    20461 tagtttcttt gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt
    20521 tcaatgtttc gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta
    20581 catattgagc agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg
    20641 cattgatttc catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag
    20701 ctaatccttg ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac
    20761 cgatcatcac gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag
    20821 taaagtgggt atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt
    20881 attgttcttg taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga
    20941 agcaaagaat actccagttc cccataagat ctttttcact gacttctaaa aattctcctt
    21001 gatgataagc atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac
    21061 ctccaataat aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg
    21121 gatattttgt tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga
    21181 ggtcgcaaga aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg
    21241 ttctttcagc atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt
    21301 atcaactaaa gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc
    21361 ctgaatggtc actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca
    21421 gttccaaact tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc
    21481 acggaaaaaa gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt
    21541 ttctagcact attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc
    21601 ttccaactgt tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac
    21661 atacgtacca ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc
    21721 ttcgcgaatg ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt
    21781 ccttccaggt tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga
    21841 ttgcaagctg cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag
    21901 aagaggtctg aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa
    21961 aattttggtt gacatagcga tttttattgc ataaaattca cttgaataca aaaactaata
    22021 tgttagttta ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa
    22081 ttatctcaaa aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga
    22141 caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca
    22201 gaaatcgctc aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa
    22261 acattcgatg aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa
    22321 gtggtcatgc caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt
    22381 ctgtattgtc atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat
    22441 cctagtttac aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca
    22501 aataataaat atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca
    22561 catacaatga gtattcttgc tgtgctactt tataaacgtt ttttatctgg agcttggccg
    22621 attgcacttg tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg
    22681 ctaacgatcg caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac
    22741 ttatcgaatg aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat
    22801 ccttccttgt cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc
    22861 caaaccgcaa aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta
    22921 atcatggaag atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg
    22981 actgatcgtg caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct
    23041 ttgcatacgt ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag
    23101 atgaaagatc cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca
    23161 gttgtggagt ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg
    23221 cgcttttcta atccaaatat tcctaatatg cctcagcgaa tcgccacgga tacctctcaa
    23281 aaggtggcga ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac
    23341 ccgcagacac ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc
    23401 attgatgatc aaggaaaacc attcatcccg agtccagacc cactattgac agaccttcag
    23461 cataagcttt ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg
    23521 gctcctattt tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc
    23581 agaaaaatag aagaaatgtt catcaagatg gtagcaggac caaatgccgt acgaaaaaca
    23641 ttacaggatt acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt
    23701 acggatcaca ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag
    23761 ggattgtagg cacattgatg gatataccag taggagaggt atggcctagg gagaggatca
    23821 aagaactaaa agaagaaatt gagtctcgtg ggttaacact aaaagtaatt gaaagcgtca
    23881 atgtgcatga tgccattaaa attggattac cttcccgtga gcagtatatc gaaaactata
    23941 agcagacaat ccgaaacttg gcagaatatg gaatagaagt catctgctat aattttatgc
    24001 ctgtatttga ctgggtgaaa tctgaccttg actaccgctt agaagacggc tcatccacgt
    24061 tagcttttat atctgatgat attccagatg atccaagaga aatcgtcgcg cgcatcgaac
    24121 attccagcaa tgaatttgga ttgccgggat gggaacctga gcgcttagct cctatcaaat
    24181 ccttgcttga agcctatgca tcagtagacg aagaaaaact tcgagaaaat tttgcctatt
    24241 ttctccaatc aatcatccca acatgtgaag aggtaggggt caaaatggct gtacatccag
    24301 atgatccgcc atactcgatt tttggattag ctcgaatcgt aaagaatcga gaagatttag
    24361 attggttgtg tcgcatagta gacagccctt ataacggaat cactctttgt acagggagta
    24421 ttgcagaaga tccagaaaat gatgtatatg ggattttagc agaattttgc caaagagatc
    24481 gaatcccatt tgcacatgtt cgaaatatta aatttttagc taatcgagat ttctatgagg
    24541 caccacataa atccgaatac ggttcattag acatgtaccg catactaaaa acaatgtatg
    24601 acaacggatt taacggatac atccgtcctg atcatggaag aatgatctgg aatgagacag
    24661 gaagaccagg atacggctta tatgatcgag cattaggtgc tgtttatcta aatggattat
    24721 gggaagcaat cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa ctgtaatttt
    24781 attatgtaaa taagaaggat gttcaaattc ttgatacata aatccggagg ttgttaataa
    24841 gcattcggat ttatgtatct tatttggtct aggaagtaac ttgattttct tttttcgatt
    24901 gatccatgag tgcttcttct tttaattcaa agatatattc tgagatcctc ataatcactt
    24961 ttttccagtc ttctggtgtg atgattcctt ttaaaatata gatttttttg atatgagaag
    25021 gcaattgttt caaagggcag tcgctgataa cgatgttgca gttctccata aaatcatttt
    25081 gtattttgat cgattcattt gaaaaaattt gttctatctt aacgatcaga tctttactga
    25141 tataaaaatc acgcgtatag ttcacaccaa tcacaattcg cggctgtttt ttgtagcgat
    25201 catacaaatg tactaggtct tcaaccatcc attgcacatt ttccttttct aaaataactg
    25261 gtaacaagtg ttgattttgt tctctgaaat ccatataaaa ttgttcaatg gcggtttgga
    25321 cttctttcaa tacgctatgt tcttgataaa gcatgaaagt aggaaggggc gtatttggtt
    25381 gattgaagtt aagaaatcca ttataaagtt tatttcgtgc gaatgccaat gagtaaagaa
    25441 tcttttcttt ttcaggtata aacaaatcaa atacagtaca aagggactcc accatttctc
    25501 ttgaataaag aacatgagga acgtttgctt tttttaaaag tttgtattgc tccaagcttg
    25561 tttcttccga ttcgattttc gtgatagaaa gtcgagctaa aatattcact aagatgcgtt
    25621 gttcgcgaca aattggcaga ttaggtcgca aaatatctac agagagatca ataaaaaagt
    25681 caatatattc atactctgtg aattcttttt gtacgtcttt cacagaagtt gcaggaaaat
    25741 tctctttaca tatttttaat agaaaatata atttatctat cataccgcta tttagtcggt
    25801 caagtacttc ttttttaaaa aaagtattca tcataaaagc cactacttgc ggatcttcat
    25861 cataaaattc tgtagcagta cttgaaaaga tgttccaata aatatctaat gctgcatatt
    25921 gaatttgtac ctcttttcca gaaaaagact ttctgccatt attagaaaag gagatagaca
    25981 aaccgtacta tgctaaaaat gcattaatct ttttcatttt cgtatacaaa taagaggcac
    26041 tatagtttgt ttgctgactg aaactgataa tcgaatttgt ctgacagctg aacaaagaag
    26101 tcaagatctg ataactgact gattcgttgc agtatcttcc aaatagacga taatagcagt
    26161 aagccggatc aattccttca aaattcaact ttaaaaaagg tttttgctta tcaatatgca
    26221 gttctgttag attgagacgc tcaaagtctt gttctaattc tttgatgtat agtaaaaaat
    26281 ttgattcaga catatggaaa tgatctgcta aatattgtgt agtaataaag aaacgatgtt
    26341 cagataggaa aagaaaaata tctaattttg tattgctttt tttcttcaag ataaacgttt
    26401 taatcgcttc ttgagtttgc atgaaagagg ctccttttta cttaaattgt taaaacttga
    26461 aaatattact gtttttttca taaatttaga caaaaaaaca gtaaacatat aggtagaatc
    26521 tctactatct tatttcttgc atacaaaaaa atcaaggagt gagagagtta tgagatattc
    26581 aaccatctta atatcaacat ttgcagcctt aagcttatcg cttactataa atcagacagt
    26641 ccacacagag gagacagaaa cagccaaatg gacagaaacc aagtgggcca acgtcaatcg
    26701 aattaccttt caggatgaat tttcatcctt caattcagga gtcgcaaaat taagaaaagg
    26761 acaaacagcg accatcaaag caaaaattga ctattcggga gacttagcac ctatactaca
    26821 atcagctgtt acatttgaga atgttgatcc cactttgtcg attggctatg acgagaacac
    26881 tttgactatc caacacaagc aagcgatatt taccttcacc gttacattaa atcaggactt
    26941 gttaaaacca gcgcttttta ctgtcaaggt atcagatgga ctaccgaacg atcatcattt
    27001 aacgccgtat agtcagcggc agacaattga gcaagattca gccatcgact caggaggaga
    27061 tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag taccgcctac
    27121 agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac ccgatacaga
    27181 aactcaacca gagccagaca ataaatcaaa acctgaaata acccctggag aaaaaccaga
    27241 tgtaactcca gaaccagata cggattctgg aaaccaaaca gtaccggaaa caaatccaga
    27301 cacagataat gaaactgaaa atccagaaaa accagaggta gatccagaag aaaaaccaga
    27361 tgtgactcca gaaccagata cggacgcaag agaccaagga ataccagaaa aaataaacaa
    27421 aaaaacaatt caagaggatg gaaaaaaaga gagtaaaaaa tcaaatctag caattctaaa
    27481 aataaatgaa gagcagctga ataaaaaatc acgaatattt gatagcgccc aatcagctga
    27541 aaccttaaaa agtagcaagg atacaacctt tgctgtcctg aaacgaaaaa taaacagcta
    27601 ccaaaatccg gagagtcaca aaacaaagtt atactgtgga gcggaatcat tctattaagt
    27661 atagcaacaa tgctttctgc aaaaagattt aaacaaaaca gatctctcta acatttgaat
    27721 aatgtctgtg aacgcatttt tcaaataaaa agaaatatcc agaaaatgac cccaaaatac
    27781 cagattgaaa tctgatattt ggggtcgttc atactttatc tttcttcata tttattttaa
    27841 gggatatctg tttcttttgg aagatggttc gggatctttt tcactttcaa tacatctaaa
    27901 aagaaggtaa agatcggaat gccgacaatc aagccccaaa cgccaaagaa ccgttcgcta
    27961 acaagtaaga ccacaaatgt ataaaaaatc ggcagttctg ttttgctgga catcaacttc
    28021 ggattcagca cataagactc aattagatgg acaatcaaaa tcgtaatcag gatataaaca
    28081 acatctcgga tgcctccttg ggaataagct atgaaagata gaggaataca agaaatgata
    28141 acacctgcta ctgggatcag acttaaaatg aaaatcatga tcgctaaact cagcagctga
    28201 tgaaagccaa atacagccaa ggcgattgta gtgagcaacg tatttaaaag agcgacaaca
    28261 aattgagctt ctaataccaa accaaatgta ttgacaaact tatcggcaaa atagtaaata
    28321 tcttgaaaga accaagaaaa ttcacttttt aaaaacaatc ttgagaatac aattgttttt
    28381 ttcttttcaa tcataaaaaa gaaacttaag atgaatgaca tagcaaacgc cactgccatt
    28441 tttcctatat cctgtaaata gccaagcgca atcgaagcgc cattttgaag ctgttcaaaa
    28501 aaatcgtttc gctcaatata agtatggata tattgtaaaa caggatctgc atcttttggc
    28561 tgatgctcgt aaaaatcaat aacggactgg atcatatcaa atgtttgatg aataagcatc
    28621 gggatatact ttgtgaatgc cagatagata aggtaaacga tcagtgtata aatcatgata
    28681 gctaaaacac ctgtagggat tcggacaaat cgctgaatga tttgaacaag atgaatcatc
    28741 aaataagtaa agataaatgt gagcaaaatg gttgtaatca tacttctaac taagaaaaga
    28801 acaaaaacga caaataataa aacagtgaga cggcgtattt tttcattcgc caaaaaacgc
    28861 tgataaacgg tcaaatcgac aacctccaaa taacaaaaaa taaacacgtt attcattata
    28921 tgatggaata tgaaaataag aaagagaaag agcaaaaaga aaaaccgcta aatgacgtta
    28981 tcttatgagt tggttttcct cataaaatcg ttcatctagc agtttcagcc ctttttctgg
    29041 tcttgatgaa aattatctag catttttcgg gtcagatgtt gcagtgtatc caattcttcc
    29101 tgttcaagtg gaagataaga cttgatcttt tgtggaatac cttctgcttg tttttgcaac
    29161 gcagcacctt gaggagtcag cgtaacattt acgattcttt catcacccgt ttcacgctga
    29221 cgattaatat aatttttttc tgctaacttt tttagtaagg gagtcagcgt accagaatcc
    29281 agacacaaat catttcctag ctctttcact gaagtctttt tcttttccca taaaactaac
    29341 atcacaagat actgggtata ggtaagattc agcggcttga ggagggaagt gtagtgtcta
    29401 atgatttcct tagaggcggc atataaagga aaacatactt gattaactaa atatagatct
    29461 tccatttttc cacctccgct gttttactat agtgtatcat agcggattta cacaaaaaag
    29521 tctattcact aactgctttc agagtaactt ctatatttcc agctgtagct tttgaatacg
    29581 gacacacttt atgcgctgcc tctaataatt cttgcgtttc ttccagagaa agaccttcga
    29641 tagatccttc aatctctaca cctaagacaa agctcgtttc actttctgaa taaagggaaa
    29701 cttgtgcact gacagtagaa gccccagaga tatttttttg ctgtttgatc aattctaatg
    29761 cactgttaaa acatgcactg aaaccagctg caaacaattg ttcagggttg gttgcatttt
    29821 cgacgcgttt accaggagct gcaatattca attcgaatgt acgatcggga ttatgaactt
    29881 ctccagagcg tccgcctgtg ttgatcattt tcgtagaata catttttttc ataaaattac
    29941 cacctttaat tatattttat acaattgaat tttacacaat ataattgtgg aaagcaaaga
    30001 aatggattgg cataagataa ataagagaaa gcacatgaaa cagacgcgct ttctcgaata
    30061 gcaaacatat acaaaaaaat atcagagaca aaaactaatc tcatcataaa cattacatat
    30121 atttctcaaa aagttgtttt ctttcttcaa aacttacttc acgatggacc tgatgaagca
    30181 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    30241 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    30301 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    30361 aaccaagaga cgtgttttca tccagcatat ggaatctcgt gccatacaag gtaaaaatga
    30421 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    30481 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatctatt tccgttcctt
    30541 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    30601 agttaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    30661 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    30721 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    30781 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    30841 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    30901 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    30961 caatagataa aatagcataa agaaactctt ctattccaaa aacaaacaga ttaagagaaa
    31021 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    31081 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    31141 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    31201 ttgtttctgg aactattgca atgattaaag gaaaaaacaa gcttccgaaa agtactttaa
    31261 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    31321 aaataactat agctcggtca aatcctaaaa cagcttccag taatattcct agaccgctga
    31381 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    31441 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatc
    31501 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    31561 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    31621 tatacttcta cgttaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    31681 cgcttaagat aaattcagga cactaaatag tacaggaagt agattaaaac ttttaaaagt
    31741 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    31801 cgataacatt tttgaaagcg ttgtcttttg tttctctatc acttatagtg atgacgtcat
    31861 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    31921 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    31981 atatcaaaaa agtatatgtg ctttgtggag atccatatac cataacaact gaaaaatggt
    32041 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    32101 ttgaagtgac gagcgaaaca aggcgcttac agtatgcgtt ccatgttgtt ggggaagata
    32161 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    32221 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    32281 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    32341 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    32401 tcttcggggg agatttgcaa ggtgtgaggg atcatttgga ttacttgacg gaccttggcg
    32461 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    32521 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    32581 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    32641 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    32701 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    32761 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    32821 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    32881 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    32941 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    33001 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcattcacag
    33061 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    33121 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    33181 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    33241 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    33301 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    33361 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    33421 ctaagattat ttctgaaggt tctactcagt ggctaatagt atatgatcgt gaagataagc
    33481 tctatttcac acgggaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    33541 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    33601 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    33661 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    33721 cagcacgttg aatacacagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    33781 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    33841 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    33901 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    33961 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    34021 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    34081 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    34141 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    34201 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    34261 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    34321 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    34381 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    34441 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    34501 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    34561 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    34621 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    34681 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    34741 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    34801 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    34861 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    34921 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    34981 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    35041 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    35101 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    35161 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    35221 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    35281 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    35341 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    35401 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    35461 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    35521 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    35581 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    35641 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    35701 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    35761 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    35821 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    35881 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    35941 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    36001 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    36061 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    36121 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    36181 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    36241 ttctccatga tctatctgtt taacaatggt ggtcccggaa gcgtaggcgg aggagctggt
    36301 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    36361 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    36421 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    36481 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    36541 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    36601 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    36661 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    36721 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    36781 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    36841 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    36901 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    36961 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    37021 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    37081 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    37141 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    37201 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    37261 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    37321 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    37381 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    37441 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    37501 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    37561 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    37621 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    37681 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    37741 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    37801 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    37861 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    37921 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    37981 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    38041 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    38101 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    38161 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    38221 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    38281 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    38341 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    38401 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    38461 cttctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    38521 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    38581 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    38641 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    38701 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    38761 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    38821 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    38881 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    38941 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    39001 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    39061 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    39121 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    39181 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    39241 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    39301 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    39361 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    39421 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    39481 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    39541 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    39601 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    39661 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    39721 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    39781 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    39841 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    39901 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    39961 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    40021 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    40081 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    40141 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    40201 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    40261 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    40321 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    40381 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    40441 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    40501 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    40561 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    40621 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    40681 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    40741 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    40801 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    40861 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    40921 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    40981 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    41041 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    41101 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    41161 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    41221 gcatgttaca gattggtaca gagcttcata aagaagggcc ggaaattttt tctagaatca
    41281 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    41341 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    41401 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    41461 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    41521 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    41581 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    41641 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    41701 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    41761 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    41821 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    41881 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    41941 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    42001 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    42061 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    42121 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    42181 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    42241 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    42301 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    42361 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    42421 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    42481 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    42541 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    42601 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    42661 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    42721 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    42781 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    42841 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    42901 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    42961 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    43021 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    43081 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    43141 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    43201 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    43261 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    43321 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    43381 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    43441 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    43501 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    43561 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    43621 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    43681 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    43741 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    43801 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    43861 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    43921 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    43981 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    44041 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    44101 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    44161 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    44221 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    44281 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    44341 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    44401 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    44461 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    44521 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    44581 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    44641 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    44701 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    44761 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    44821 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    44881 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    44941 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    45001 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    45061 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    45121 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    45181 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    45241 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    45301 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    45361 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    45421 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    45481 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    45541 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    45601 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    45661 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    45721 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    45781 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    45841 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    45901 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    45961 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    46021 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    46081 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    46141 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    46201 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    46261 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    46321 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    46381 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    46441 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    46501 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    46561 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    46621 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    46681 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    46741 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    46801 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    46861 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    46921 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    46981 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    47041 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    47101 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    47161 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    47221 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    47281 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    47341 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    47401 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    47461 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    47521 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    47581 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    47641 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    47701 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    47761 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    47821 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    47881 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    47941 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    48001 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    48061 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    48121 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    48181 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    48241 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    48301 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    48361 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    48421 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    48481 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    48541 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    48601 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    48661 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    48721 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    48781 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    48841 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    48901 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    48961 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    49021 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    49081 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    49141 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    49201 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    49261 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    49321 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    49381 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    49441 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    49501 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    49561 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    49621 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    49681 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    49741 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    49801 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    49861 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    49921 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    49981 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    50041 atgatacagt tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac
    50101 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    50161 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    50221 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    50281 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    50341 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    50401 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    50461 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa
    50521 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    50581 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    50641 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    50701 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    50761 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    50821 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    50881 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    50941 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    51001 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    51061 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    51121 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    51181 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca
    51241 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    51301 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    51361 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    51421 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    51481 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    51541 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    51601 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    51661 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    51721 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    51781 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    51841 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    51901 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    51961 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    52021 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    52081 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    52141 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    52201 tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac
    52261 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    52321 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    52381 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    52441 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    52501 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    52561 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    52621 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    52681 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    52741 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    52801 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    52861 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    52921 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    52981 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    53041 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    53101 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    53161 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    53221 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    53281 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    53341 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    53401 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    53461 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    53521 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    53581 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    53641 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    53701 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    53761 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    53821 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    53881 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    53941 aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga
    54001 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    54061 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    54121 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    54181 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    54241 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    54301 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    54361 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    54421 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    54481 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    54541 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    54601 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    54661 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    54721 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    54781 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    54841 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    54901 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    54961 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    55021 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    55081 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    55141 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    55201 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    55261 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    55321 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    55381 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    55441 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    55501 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    55561 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    55621 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    55681 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    55741 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    55801 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    55861 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    55921 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    55981 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    56041 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    56101 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    56161 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    56221 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    56281 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    56341 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    56401 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    56461 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    56521 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    56581 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    56641 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    56701 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    56761 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    56821 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    56881 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    56941 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    57001 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    57061 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    57121 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    57181 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    57241 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    57301 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    57361 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    57421 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    57481 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    57541 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    57601 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    57661 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    57721 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    57781 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    57841 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    57901 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    57961 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    58021 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    58081 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    58141 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    58201 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    58261 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    58321 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    58381 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    58441 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    58501 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    58561 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    58621 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    58681 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    58741 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    58801 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    58861 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
    58921 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    58981 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    59041 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    59101 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    59161 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    59221 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    59281 taattccttt tattcaaaaa acataccaaa aata
//
LOCUS       DAZKOU010000006        56380 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000006 DAZKOU010000000
VERSION     DAZKOU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.006"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..1683
                     /locus_tag="ACG827_000314"
     CDS             52..1683
                     /locus_tag="ACG827_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0482486.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1745..2386)
                     /locus_tag="ACG827_000315"
     CDS             complement(1745..2386)
                     /locus_tag="ACG827_000315"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0482487.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2572..2648
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2668..2976
                     /gene="rplU"
                     /locus_tag="ACG827_000316"
     CDS             2668..2976
                     /gene="rplU"
                     /locus_tag="ACG827_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0482488.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            3003..3347
                     /locus_tag="ACG827_000317"
     CDS             3003..3347
                     /locus_tag="ACG827_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0482489.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3360..3653
                     /gene="rpmA"
                     /locus_tag="ACG827_000318"
     CDS             3360..3653
                     /gene="rpmA"
                     /locus_tag="ACG827_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0482490.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4087..6894
                     /locus_tag="ACG827_000319"
     CDS             4087..6894
                     /locus_tag="ACG827_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0482491.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7085..7576
                     /locus_tag="ACG827_000320"
     CDS             7085..7576
                     /locus_tag="ACG827_000320"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0482492.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7679..8668
                     /locus_tag="ACG827_000321"
     CDS             7679..8668
                     /locus_tag="ACG827_000321"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0482493.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8701..9504
                     /locus_tag="ACG827_000322"
     CDS             8701..9504
                     /locus_tag="ACG827_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0482494.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9525..10439
                     /locus_tag="ACG827_000323"
     CDS             9525..10439
                     /locus_tag="ACG827_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0482495.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10522..10896
                     /locus_tag="ACG827_000324"
     CDS             10522..10896
                     /locus_tag="ACG827_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0482496.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11035..11280
                     /locus_tag="ACG827_000325"
     CDS             11035..11280
                     /locus_tag="ACG827_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0482497.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11452..12516
                     /locus_tag="ACG827_000326"
     CDS             11452..12516
                     /locus_tag="ACG827_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0482498.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12762..13187
                     /locus_tag="ACG827_000327"
     CDS             12762..13187
                     /locus_tag="ACG827_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0482499.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13180..13629
                     /gene="nusB"
                     /locus_tag="ACG827_000328"
     CDS             13180..13629
                     /gene="nusB"
                     /locus_tag="ACG827_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0482500.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13726..14580
                     /locus_tag="ACG827_000329"
     CDS             13726..14580
                     /locus_tag="ACG827_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0482501.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14581..15918
                     /gene="xseA"
                     /locus_tag="ACG827_000330"
     CDS             14581..15918
                     /gene="xseA"
                     /locus_tag="ACG827_000330"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0482502.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15923..16147
                     /locus_tag="ACG827_000331"
     CDS             15923..16147
                     /locus_tag="ACG827_000331"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0482503.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16149..17039
                     /locus_tag="ACG827_000332"
     CDS             16149..17039
                     /locus_tag="ACG827_000332"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0482504.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17048..17866
                     /locus_tag="ACG827_000333"
     CDS             17048..17866
                     /locus_tag="ACG827_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0482505.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17973..18422
                     /locus_tag="ACG827_000334"
     CDS             17973..18422
                     /locus_tag="ACG827_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0482506.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18440..20122
                     /gene="recN"
                     /locus_tag="ACG827_000335"
     CDS             18440..20122
                     /gene="recN"
                     /locus_tag="ACG827_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0482507.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20177..20293)
                     /locus_tag="ACG827_000336"
     CDS             complement(20177..20293)
                     /locus_tag="ACG827_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0482508.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20366..21322)
                     /locus_tag="ACG827_000337"
     CDS             complement(20366..21322)
                     /locus_tag="ACG827_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0482509.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21493..21861
                     /locus_tag="ACG827_000338"
     CDS             21493..21861
                     /locus_tag="ACG827_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0482510.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22031..22462
                     /gene="mraZ"
                     /locus_tag="ACG827_000339"
     CDS             22031..22462
                     /gene="mraZ"
                     /locus_tag="ACG827_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0482511.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22483..23442
                     /gene="rsmH"
                     /locus_tag="ACG827_000340"
     CDS             22483..23442
                     /gene="rsmH"
                     /locus_tag="ACG827_000340"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0482512.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23447..23848
                     /gene="ftsL"
                     /locus_tag="ACG827_000341"
     CDS             23447..23848
                     /gene="ftsL"
                     /locus_tag="ACG827_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0482513.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23849..26041
                     /locus_tag="ACG827_000342"
     CDS             23849..26041
                     /locus_tag="ACG827_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0482514.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26067..27029
                     /gene="mraY"
                     /locus_tag="ACG827_000343"
     CDS             26067..27029
                     /gene="mraY"
                     /locus_tag="ACG827_000343"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0482515.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27043..28404
                     /gene="murD"
                     /locus_tag="ACG827_000344"
     CDS             27043..28404
                     /gene="murD"
                     /locus_tag="ACG827_000344"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0482516.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28430..29518
                     /gene="murG"
                     /locus_tag="ACG827_000345"
     CDS             28430..29518
                     /gene="murG"
                     /locus_tag="ACG827_000345"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0482517.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29533..30753
                     /locus_tag="ACG827_000346"
     CDS             29533..30753
                     /locus_tag="ACG827_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0482518.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30909..32234
                     /gene="ftsA"
                     /locus_tag="ACG827_000347"
     CDS             30909..32234
                     /gene="ftsA"
                     /locus_tag="ACG827_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0482519.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32257..33498
                     /gene="ftsZ"
                     /locus_tag="ACG827_000348"
     CDS             32257..33498
                     /gene="ftsZ"
                     /locus_tag="ACG827_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0482520.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33515..34192
                     /locus_tag="ACG827_000349"
     CDS             33515..34192
                     /locus_tag="ACG827_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0482521.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34206..34805
                     /locus_tag="ACG827_000350"
     CDS             34206..34805
                     /locus_tag="ACG827_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0482522.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34886..35107
                     /locus_tag="ACG827_000351"
     CDS             34886..35107
                     /locus_tag="ACG827_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0482523.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35156..35938
                     /locus_tag="ACG827_000352"
     CDS             35156..35938
                     /locus_tag="ACG827_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0482524.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35959..36666
                     /locus_tag="ACG827_000353"
     CDS             35959..36666
                     /locus_tag="ACG827_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0482525.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36928..39726
                     /gene="ileS"
                     /locus_tag="ACG827_000354"
     CDS             36928..39726
                     /gene="ileS"
                     /locus_tag="ACG827_000354"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0482526.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39775..41301)
                     /gene="zwf"
                     /locus_tag="ACG827_000355"
     CDS             complement(39775..41301)
                     /gene="zwf"
                     /locus_tag="ACG827_000355"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0482527.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41316..41963)
                     /locus_tag="ACG827_000356"
     CDS             complement(41316..41963)
                     /locus_tag="ACG827_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0482528.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42147..42476)
                     /locus_tag="ACG827_000357"
     CDS             complement(42147..42476)
                     /locus_tag="ACG827_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0482529.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42653..43381
                     /gene="sfsA"
                     /locus_tag="ACG827_000358"
     CDS             42653..43381
                     /gene="sfsA"
                     /locus_tag="ACG827_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0482530.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43397..44410
                     /locus_tag="ACG827_000359"
     CDS             43397..44410
                     /locus_tag="ACG827_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0482531.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44410..45687
                     /locus_tag="ACG827_000360"
     CDS             44410..45687
                     /locus_tag="ACG827_000360"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0482532.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45750..48452
                     /locus_tag="ACG827_000361"
     CDS             45750..48452
                     /locus_tag="ACG827_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0482533.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48604..48921
                     /locus_tag="ACG827_000362"
     CDS             48604..48921
                     /locus_tag="ACG827_000362"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0482534.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48951..49271
                     /locus_tag="ACG827_000363"
     CDS             48951..49271
                     /locus_tag="ACG827_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0482535.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49379..50839
                     /locus_tag="ACG827_000364"
     CDS             49379..50839
                     /locus_tag="ACG827_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0482536.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50907..51128
                     /locus_tag="ACG827_000365"
     CDS             50907..51128
                     /locus_tag="ACG827_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482537.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51159..51341
                     /locus_tag="ACG827_000366"
     CDS             51159..51341
                     /locus_tag="ACG827_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0482538.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51341..51754
                     /locus_tag="ACG827_000367"
     CDS             51341..51754
                     /locus_tag="ACG827_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0482539.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51877..53058
                     /locus_tag="ACG827_000368"
     CDS             51877..53058
                     /locus_tag="ACG827_000368"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0482540.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53129..53293)
                     /locus_tag="ACG827_000369"
     CDS             complement(53129..53293)
                     /locus_tag="ACG827_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482541.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53447..53520
                     /locus_tag="ACG827_000370"
     tRNA            53447..53520
                     /locus_tag="ACG827_000370"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53481..53483,aa:Arg,seq:cct)
     gene            complement(53589..54728)
                     /locus_tag="ACG827_000371"
     CDS             complement(53589..54728)
                     /locus_tag="ACG827_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0482542.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(55027..55662)
                     /locus_tag="ACG827_000372"
     CDS             complement(55027..55662)
                     /locus_tag="ACG827_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482543.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(55775..>56380)
                     /locus_tag="ACG827_000373"
     CDS             complement(55775..>56380)
                     /locus_tag="ACG827_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0482544.1"
                     /translation="VLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFTTVGFSLSSSN
                     EANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSYYSITPATEQN
                     TAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKVSYVIFAKDLN
                     NADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT        18494 a         9556 c        12020 g        16310 t
ORIGIN      
        1 tattctaatg aatggtattt tagaaaattt catgaaaaag gagttagata ggtgttcaaa
       61 aattatgttt cctcactaaa aaaagaattt tcaggttatg atctttcaag atttcagaaa
      121 gatcttttgg ctgggatcac agtagctgct gtagcattgc ctttagcctt ggcttttggg
      181 gtatcaagcg gagctgatgc ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt
      241 attggcggtt tatcaggcgg tttttatcaa atatcaggtc cgacaggtgc gatggcagct
      301 atcttgatgt ctattgcggc aacaaaggga atgcaaggtg tattgcttgc tactttttta
      361 gcgggtctat ttttacttgc agcaggactt ttccgattag gaacactgac ttcttttatc
      421 ccagcacccg taattacggg gtttacttca ggtattgcta tcattattgc attaggtcag
      481 atcgataatt tatttggtgt tcactcggaa ggtacaaacg ttattgaaaa actaagtagt
      541 tatcaaacga taggatttcc gataaacaca gcctctttga tcatgggagg gctagtcatt
      601 ttagggatgt ttatctatcc aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct
      661 atcattttgg caacagcagt tatgctattg ttccatttac cagtagcaac tgtgggtaag
      721 attccacaaa cattgatcag tagtaatcgt ttgaatatgg gagattttag cttttctgcc
      781 ttgcaacaag tggcagttcc agctattagc attgctttat taggaatgat tgaaagcttg
      841 ctatgtggtg cttcggctgg ccgaatggca aataagccct tagatagcaa tcaagaatta
      901 atggcgcaag gaattggaaa tctgttgctt cctttctttg gcgggattcc tgctactgcg
      961 gccatcgccc gtacaagcgt ggccatcaaa tctggtgcac aaactagatt agctggtatg
     1021 atacatgcag ttgtcttact gctctccatg cttgtttttg cgccagtgat gtcacagatt
     1081 ccaatgccag cacttgcagg tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa
     1141 acgatcaagg agctatttac taataaatat tggtcagcag ttctattgtt tatccttacg
     1201 atgatttgta cagttatctt tgatttaagt attgctatcg tgatcggggt gatcggcggc
     1261 tgtgtatttt ttgttgtcaa aagcgctgct atcacgattt cattagaaac gatcgattgg
     1321 caaagaatgg ggctgccaga aacggaaaag ctgaataact ggacagtagt gtatattagt
     1381 ggtccactgt tttttatgtc ttcagaaaaa ctcaaaaata aattggaaga actaaatcat
     1441 cacgatggtg tcattttctc catgcgaggc gtaccaagta ttgatttgac ggcacgtgca
     1501 gtttttgatg aatttcaaga aaaagcagag caaagaggac aaacgattat ctatacttcc
     1561 ttgcagccag aagtagaaaa acaactttct cacttgtggg aacatacatt cacgcatcaa
     1621 catcaaacag ttgctcatgc gttgacagct ttacatgcga aatatactgc aattagccat
     1681 taaaacaatg aaaaagaaca gacaagactg taaactgaag attttatggt cttgtctgtt
     1741 ctttttaata ttgctgtaaa agtgaagcta gtacttgcca gtcttgatgg ataacaggta
     1801 caagggagcg attgtagaaa atagctgctg gatgaaaaag tggaagaatt cgatagtttt
     1861 tttctgacca ttcgtaggca tccttttgtt ctgttaattg taagacaggc cctgtataga
     1921 gttgaccatg atttttggta accgtatatt ctttgccaag taaacgttga agaccaatgt
     1981 ttccaagtgt cgcaattaat tttggctgga ccttcatcag ttcgtaatcc aatattggtg
     2041 catgcgcaaa tacttcggaa cgagtaggtg tccgatttgg ataaacagtt tctgactgtt
     2101 gtgtccgttt attgatccga tgagtcacgc gatacgggcg gctccttaca gcactggtga
     2161 tatacacctc ttctcgtgtt aagccagttg aagaaagtgc ctgcatcaat tcttttccag
     2221 cttgaccgct aaaaggaaca tgcgtatcaa tctctgtttt ccctggggct tctcccacga
     2281 gcatcagttt aggatgaaga ggtccttgcc cagcgataaa tccttctaca ggacggccgt
     2341 tcattcttaa agaagcttct tgtatcagtt cttttgggta ttccatttat gctcctcctt
     2401 tttcttttat tataagaata aaagaaacac aatgcgacta ttttatttgg tagaactgtg
     2461 aggatagaaa agcagaagta ttgcttgaat ttttgtttta ctttttgatt ttaaatttag
     2521 ttgactaacg gttgctgttc tggtaagcta gtactgttgt aattgtggac tccacagcta
     2581 caaccgctca gaacaagtaa taagcacagc atatgctgtc acttcggatg gcgagtctaa
     2641 gtatatcaga aggaggtgct ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa
     2701 gtaaaagttg aagtgggaca agcaatctat gttgaaaaac ttgacgttga agcaggagaa
     2761 aaagttgttt ttgacgaagt tatcttagta ggcggagaat ctacaaaagt aggcgctcca
     2821 actgttgcag gtgcaactgt tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc
     2881 gttactttca aatacaaacc taaaaaacat tcacaccgta aacaaggtca tcgtcaacca
     2941 tatacaaaag tggttatcga cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat
     3001 atatgatcaa aggaacattt aagcgaaatg acgcaggtca gatcgtttca ttcactttga
     3061 ccgggcatgc tgatgctggt ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt
     3121 tagcgatcag cactgtcaac gggattgcct cattagccgg ttttgagccg attgttgaaa
     3181 tgaatgaaga agaaggcggt tatctttata ccgaagtcac ttcaggaatg acacaagaac
     3241 aaaacaatat tgctgagatt ctattagaaa atcttttatt aggattacaa tcgatagaag
     3301 ctgaaaactc agaatatatc caaattaaaa caattaatga taaataggag gtgcagacca
     3361 tgttgttatc tatgaatcta caattatttg cccacaaaaa aggtggcggt tctacatcta
     3421 acggccgtga ctcagaatca aaacgtctag gtgctaaacg tgctgatgga caaacagtta
     3481 caggtggatc aattctttac cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg
     3541 gtatcggtgg agacgacact ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta
     3601 aaggccgtaa caaaaaacaa gtgtctgttt atccagtagc tcaagaagct taattgaatc
     3661 ttatgattaa agaggttgtg acaagacttt tgtcacagcc ttttttgttt ataaaaataa
     3721 aagttgtagt ggaggctacg aagtttactt tgtcttttta cacttaatat ttttttgcca
     3781 atttggaatc aggatgtttt gataactatc gttttcaatt gatggaagct atattgtttg
     3841 ctatcagtga aataattata tggtaaacag tgttcttttt cttgtttcca ttataaaaat
     3901 cgtatcggga tttttcgttc ttttaaattg aacatttcta tctatccgcg tgttatcaac
     3961 aaaacaaaat caggtaaata gttgacgaaa cagaaataaa tacatagtat tatgttttga
     4021 agcctaagta caatagtcta tagaaaagga gcgataccat tggcaaaacg cattgaccga
     4081 atttatatgt atgtcaaaga aaaaaccgct catttaacaa aggcagaata tgatcaagga
     4141 gtaacaacac aggaaattgc tgaagtgtta gggattcagc gaacaaatag cagtaaagat
     4201 ctgaatcagc tagtgcgcga aggaaaactg ataaagacag acggacgacc agtacgttac
     4261 atatatcaaa cactcgttac gcacgtaaag ccagttacaa aacatgtagt cagttacaaa
     4321 gaaacgccgg tctctgaaga aaaaaataca ctccaaatag atactaagga tatctttgca
     4381 aaaatcattg gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt
     4441 ttgtatccgc caagaggatt aaattgtttg attacaggac ctaccggatc aggaaagact
     4501 tattttgcac acgcgatgta tcattttgca aaagcgcatc atgtaataga tgaggaaacg
     4561 gaactgattg ttttcaattg tgctgattat gccaataacc ctgagctttt gatgagtcat
     4621 ttatttgggt acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat ggggattatc
     4681 gatcaagcgg atggcggtat gctgtttttg gatgagattc accggttgcc gcctgaaggt
     4741 caagaaatga tcttttactt tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg
     4801 aaatcccatg aagcaaatgt acggatcgta ggtgcgacca cagaagaccc aggttcttct
     4861 ctattagaaa cttttgttcg gcgtatccca atcaatatca aacttccggc ttttgaaaaa
     4921 cgtcctgcaa atgagaaagt cgatttagta aaaatcatga ttgcccacga agcaaaccgc
     4981 acgcagcgaa agatttcttt gacagaagat gtagcaaaag cattgatcgg cagtgtgacc
     5041 tatggaaata taggacaatt gaagtcaaat atccagttag tatgtgcacg aggatttcta
     5101 aatcatatgc aatcacccga aatctcgatc acaatcgatg atttgacaga agggattcgt
     5161 agcggattga ttcaattagc aagcaaccgc agtgcaatgg cagagctttc taaaatattg
     5221 gagccaaaga taactgtagc accaaatgat acgatcatga aaatccaatc ggactcctat
     5281 gagttacctt ataatctata tgatatcatc ggagataaag cagctttact gaagtcggat
     5341 ggtttggatc aagaagcaat caatcatttc atttctacag atatcaacat ccatctgaag
     5401 tctttctata aagaccatgg tttttctttt aatgcagaca gcaaattagc agaatttgta
     5461 gatccgaaaa tcatagaagt gaccaatcag atttatgtaa tggtcaaaag atctttacca
     5521 tatgagtttc agcaaaactt tatttatgct atgagcttgc atatcagttc ttttttgaaa
     5581 aaaatcaact taggagaaga acggcatacg aatgataaca tccgtgagat ggcttttgat
     5641 tttccagatg aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa
     5701 gtggagattc cagaaagtga agactattac ttgaccgttc tactcgtctc gttacgggca
     5761 aatcaagcaa gcggcagaat cggtgtagtc gttgcagctc acggaaacag cacagcaagc
     5821 agtatggtcc aagttgtcca acagttgtta gatgcagaga acgtacgagc agtagacatg
     5881 cctttggata tggatccgaa aacagcattg gaccgaattg aacgcaatgt acaagaagta
     5941 gatgaaggaa gcggggtcat actcttagta gatatggggt cccttgcgtc gtttaattcg
     6001 caaattcaaa gagacactgg aattccagta cgtactgttg atatggtaac aacttctcta
     6061 gttttggaaa ctgtacgaaa ggtctcagta ttagggacag atctagatat gttgcatgaa
     6121 tcattgaaaa attttcgagg ttacgcagaa atatcttctg tagaatctgt tcaggaagca
     6181 aaacctattc ataagaaaaa agctgttttg gctatctgcg catctggcga aggtacagca
     6241 caaagaatca aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa
     6301 gttctagctt tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca aaaagaatat
     6361 cagatcatag ctactactgg gattactgat ttgaatatcc aagccccatt tattccttta
     6421 gaacgtttta ttgatcaaaa tatcgaagtc attttggatc agttgctgat ggaatcagaa
     6481 ttggaggaaa ctgaattcat ctccctagat gaagagtcag ctaaaaatac gtgtgttgag
     6541 tttatctctg ataacttcat ctttattaat ggtagtaagt taatcgatcc aatgtggcaa
     6601 ttttctacac aaatctctca gacgacggga atcggcgatg aagaatatgg attcaaaatc
     6661 aacctagtga tgcacacagc aggaatgatc gaacgtatta ttcggaatga accattgact
     6721 gtggaagaaa acgaattgac aaatacaacg aatgatcctc tttatagcca attggcagcc
     6781 tcagttgttc tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga aatgtattat
     6841 ctgctacgtc tagtccataa tcagttagat aaaaaagaat acactgtgcc atgaatacac
     6901 aaaaacgata cacttttata gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt
     6961 caattagtgt atcgtaaaaa agttggcacg ctttttgctt atagtagtgt gtgctaggag
     7021 gtggttgttc acacagaaat gataggggtt tctgtgaagt agtgaggaag aaggagtttt
     7081 gcagatggat attcgattgg cacgtataga tgatcgttta atccacgggc aagtcgcaac
     7141 agtttggtcg aagcaaacag gtgttgaacg tatcattgtg atcagcgatg tagtcgctca
     7201 agataaattg agaaagtttc tactaaaaga agctgcaccg ccggggatca cagcaaatgt
     7261 aataaccgtg cagaaaatga ttcaaatcta tcatagtgga ttattatcac gagcaaaagt
     7321 gatgctttta ttcaccaatc cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt
     7381 gaagtcgttg aatatcggcg gtatgagttt tactgaaggc aaatcaatga ttaccaattt
     7441 tgtttcagta aatcaacaag atattgattc ctttcgttac ttgaacagtc aagaagtgga
     7501 gttagagatt cgaaaagtac ctgctgacag gaaaatcctt ttgatggaac tactagcgaa
     7561 aatcaaaaag gtgtagcaag aatggttgtt ggaaaaggtc aaataattct gttgtaatgg
     7621 ttagcaatta ttaatatttt tgaatttgtt acaacaaaag taaataggag gtataaatat
     7681 ggtagggatt atcctagcaa gtcatggaca atttgctgaa ggaatcttac aatccggttc
     7741 aatgatcttt ggcgaacaag aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc
     7801 agatgacttg agagccaaat tggaagaagc ggttgcttct tttgataatc aagacgaagt
     7861 gttattctta gttgatttat ggggcggaac accgttcaat caatcaaaca cattattcga
     7921 agagcacaaa gataaatggg caatcgtcag cggtttgaat ttaccaatgt tgattgaagc
     7981 ctacgcatca cgcttttcaa tggaatctgc tcatgaaatt gcagcacaca tcattgaaac
     8041 agcaaaagac ggtgtaaaag taaaaccgga agaattagaa ccggcagaag caccgaaagc
     8101 agcagttgaa gacgcacaac ctaaaggagc acttccagaa ggaactgtgg tcggagacgg
     8161 aaaaatcaaa tatgtattag cacgtgttga ctcacgtctg ttacatggac aagttgcgac
     8221 agcttggaca aaagcagtgc aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa
     8281 ggatgatctg cgtaagagac taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt
     8341 tattcctatt agcaaaatga tcgaagtagc aaaagaccct cgttttggaa ataccaaagc
     8401 attgctttta tttgaaaatc ctgaagacgt attgacagct gttgaaggtg gcgtagacat
     8461 caaagaatta aatgttgggt caatggcgca ttcggtcggt aaagtcgttg taagtaaagt
     8521 attgtctatg ggacaagaag acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa
     8581 attcgatgta cgtaaagtac ctaatgattc acgcgataac atggatgata ttttgaaaaa
     8641 agcaaaagct gaattagcaa aagcgtaata aataatatta aaatttatag gaggcttatc
     8701 atgtctatta tttcaataat tttagtcgta ttagttgcct ttctagctgg tatggaaggt
     8761 attcttgatg aatttcaatt tcatcaacca ctagtggcat gtacattgat cggtttagtt
     8821 accggtaacc tcgaagctgg tatcgtactt ggcggtacgc ttcagatgat cgctttaggt
     8881 tgggcaaata tcggtgccgc tgtagcacca gatgctgcat tagcatcagt agcatcagca
     8941 attattttag ttttaggtgg acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc
     9001 gttcctttgg ctgttgcagg gttgttctta acaatgatcg tacgtacaat cgctgttcca
     9061 atcgttcatt tgatggatgc ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg
     9121 cacatcattg ctgtatgttt acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta
     9181 tttatcccag cacaaactgt gcaatcattc cttgaatcaa tgccagcatg gttaacagat
     9241 ggtatggcta tcggtggggg aatggtcgta gctgtaggtt acgctttggt aatcaacatg
     9301 atggctacaa aagaagtatg gccattcttc gttattggtt tcgttgtagc tgcaatttct
     9361 caattaacat tgattgctct aggtgcttta ggtgttgcat tagctcttat ttacttgaac
     9421 ctttctaaaa tgggtggttc atcaaataat ggaggcggaa gcaactctgg tgacccttta
     9481 ggcgacattt tgaatgacta ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa
     9541 tcaaattatc aaaaaaagat cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt
     9601 catggaacta cgaacgtatg caaaacggtg gctgggcatt ttctatgatt ccagcaatca
     9661 aaaaattata caaaacaaaa gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct
     9721 ttaatacaca cccatacatt gcttctccaa ttttaggggt tacattggct ctggaagaag
     9781 aacgtgcgaa tggcgcgcct gttgatgatg tagcgattca aggggttaaa gtaggtatga
     9841 tgggtccttt agctggtgtt ggtgacccag tgttctggtt tacagtacgt ccaatgctag
     9901 gtgcactagg tgcttcatta gctatgggag gaaatatcct tggaccaatc atcttcttct
     9961 taggttggaa cattattcgt tgggcattca tgtggtatac acaagaattt ggctacaaag
    10021 ctggttctaa aattactgat gacctttctg gcggattgtt acaagacgta acaaaaggtg
    10081 cttcaatttt agggatgttc gtattagctg cccttgtaca aagatgggta tcaatcaaat
    10141 tcttaccagt tgtatcaact gtaaaattag ataaaggtgc ttacatcgaa tgggataaat
    10201 tgccttcagg tggtgaagga atcaagagtg catttgaaca agttaataat ggcttagccc
    10261 tttctcctga aaaggtaaca acattacaag acaacttaga ccaattgatt cctggtttag
    10321 ctgcattagg attaacattg ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg
    10381 ttatcatcct tggtttattc gtagtcggtg ttgttggaca cgtcatcgga cttttataat
    10441 ccataaaaat atagctcggg cgttgtcccg agcttttttt atatagtata ctgttcataa
    10501 acaaatatct ggagggagaa aatggttcaa tcaatcaata caaaagtaga cttggtaatt
    10561 gatgctactt cgtttacggg tctgacagat tacgggcaaa tcatgatagg taataatggt
    10621 tttgagtttt accatgcgcg agatagacgc aaatatatcc aaattccatg ggaagaagtg
    10681 gattatgtac tagcatcagt catgctaaag ggaaaatgga ttcctcgata tgccattcaa
    10741 acgaaaaaaa atggtacata cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg
    10801 atcagagaat atgtagatga agatagaatg gtacaatcat tgagtttctt tgatgtagtg
    10861 aaacgtggta ttcgtagtat gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg
    10921 aggttttctt tattttttta ctttgcagaa gaaaatatga aatagcaaaa ccaattattt
    10981 gtccccttta aatcccatat gctaaaattg attagcaaag aaggaggtta tattatgagt
    11041 tggttatgga cattgattgt tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt
    11101 aaaggaaatt cgatgggctg tgtaacgaat atcattgctg gactagtcgg ttcaagtatt
    11161 ggacaagcga ttttcggcga atggggacca aaagctgcag gtatggcact tattccatct
    11221 attttaggcg cagtgatttt ggtagcagtt gtctcatttt tctttggaaa acgatcataa
    11281 gtttttacaa aatagtaaac taaaaggagt tgcagcgaga ctgtgactcc tttttggctt
    11341 tatttattta aaataaaaag aagttttccg gaaattttta tttaactgtc gtgtacgaag
    11401 aaaagtttgc tataatggaa aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta
    11461 cgtgtagaaa aattgcgcaa aaaaatgcaa gaagaaaatt tggattcatt tttggttacc
    11521 agtccgtaca atcttcgtta cttaacgaac tttacaggta cgacaggatt agctgttatt
    11581 actttggaaa aagcattttt tattactgat tttcgttata ccgagcaggc ggcagcgcag
    11641 gctcaagggt ttgaaattat taaaaatgtc ggacctattt ttgatgaggt agcaaactta
    11701 gtacgcaaag aagaactgca ctcattagct tttgaggaaa caacagtctc atttttagaa
    11761 tattcggttt tagaagaaat catcgatgcg gaattagtac ctgtttcaca gatgatcgaa
    11821 gaactcagag aagtgaaaga tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt
    11881 gcggatatgg catatgatca catcctgaaa atgatccaac cgggcatgac tgagattgaa
    11941 gtcgcaaatc aactagactt ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat
    12001 acgatcgttg ctagcggatt gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc
    12061 atcgaacaag gtgacttgat cacattggac tttggttgct attacgaagg gtacgtttcc
    12121 gatatgacac gtacattcgc tattggtgat cctggggaaa aattaaaaga aatttatcaa
    12181 atagtcttag atgctcaatt agcagttatt gatgctgcaa aaccaggaat gactggtgtc
    12241 caattagatg cagttgctag agattatatt tcaaaacatg gttacgggga agcatttgga
    12301 cattctacag gacatgggat cggtttagag atccacgaag gaccaaacgt ttcttctcgt
    12361 gcagaaaagc aatttgtacc aggaaatgtg atcactgatg aaccagggat ctacttacca
    12421 ggaatcggtg gagtacgtat tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg
    12481 ttgactcgtt caccaaaaga attgatcatc ttatagtttc atcaatagaa gaagtaagcg
    12541 taaaaatcag tgttcacaga ctttgttata gaaaatcact cgatttaatg atacactata
    12601 gaagaacttt taagctgtaa gagataataa agttgggtgt tttctccaat tcatagcagt
    12661 ttgcagctga tcggcggcag ttttcttacc acgtgatcac tatgccgcat catagagaat
    12721 tcggatgtat ctcgtgctta ggcaaaggga ggaataaata aatggcagat gaaaaaaatt
    12781 tgattttaaa cgcaaaccaa gatttaggag agatcgtgat cgctcctgaa gtcatcgaag
    12841 taattatcgg gattgcagct tctaaagtag aaggcgtata cggaatgcgc ggaacttttg
    12901 ccagcaatgt cactgaatta ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa
    12961 acgaggaaga tggcttgaaa gtagatcttt actgttatct agaatatggt gtttcagttc
    13021 ctaaagtagc aatggaaatg caggaacggg tcagccagca agtccttttc atgacggata
    13081 tcgatttatc tgaagtcaat atccatgtag tagctgttgt tcctgaaaaa ttagaacaac
    13141 caacaatcga agaacttttt gaagacgaag aggaagagaa tgaataaaga attatcaaga
    13201 cacgagattc gggaaatggc attgcaggct ttatttccat tagacttcaa tgcagaccta
    13261 acaaaagaag atgcgatttt caacgcaatc gaattagacc accgcgacat gatcaacgaa
    13321 gatgaatcag agtttgttcc tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa
    13381 aaagatgaat tggataaagt gattgaaaaa catttaaaaa acaattggca catcaatcgg
    13441 atctcaaaaa tggatttggt gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca
    13501 gatgtaccag cggctgttgc tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat
    13561 gaccgttcac gtaaattcgt caacggtgtt ttatccaatg tattgaaaga attagaagct
    13621 ggggcgtaag tcctagcttt tttctatgaa tcatcataaa cctgttataa atcgggaaaa
    13681 ttctatggta gaatgaaaac acaaaaaata tctgggagtg gttagatggc agaattgatt
    13741 aacggaaaag aattagctga aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg
    13801 aaagataatg gtatccatcc cggattagtc gttttattgg taggggaaaa tccagccagc
    13861 caaatctatg taagaaataa agaacgtgta gcaaaagaaa tcggaatcca ttcattggta
    13921 gagcgttatc ctgagacgat ttctgaagac gaactacttg cagaaattga aaaatacaat
    13981 caagatccta gttttcatgg tatattggtc caattaccat tgccaaagca tatagatgaa
    14041 gaaaagatcc tgttagctat ccgccctgaa aaagacgtag acggttttca tccgatgaat
    14101 ttaggcagac tccttgcggg taaaccggac aagattcctt gtactccata cggcatcatg
    14161 aaaatgtttg aagcttattc tatcgatccg gcgggtaaac gagcagtagt tatcggacga
    14221 agcaatatcg tcggtaaacc gatggctcag ctactcttga tggcagatgc aactgttact
    14281 gttgctcatt ctaaaaccga aaatctggct gaactggcac aagaagcaga tattttagtc
    14341 gtagcaatag gacgtggaca ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg
    14401 atagatgtag ggatgaatcg tgatgctaac ggtaagctga tcggtgatgt gaaatttgat
    14461 gaagtggagc ctattgcagg ctggatcact cctgtgccaa agggtgtagg gccaatgact
    14521 atcacgatgc tgatgtatca aaccgtaaaa tctgccgaag cttttggagc aggtgaatag
    14581 atggcacaag aatatttaac tgtcactaca ttaacgaagt atttgaaaag aaaatttgat
    14641 gccgatcctt atttagaacg cgtatattta acaggcgaaa tatcgaattt ccgtttacgt
    14701 gccaatgccc atcaatattt cagcttaaaa gatgaccatg caaaaatatc tgctattatg
    14761 ttcaaaggag cattccaaaa attacgattc cagccaaaag aaggaatgaa agtccttgtc
    14821 gttgggcgta tttcattata tgaagcggga ggttcttatc aaatctatgt agagcacatg
    14881 gagcctgatg gaataggtgc tttatatcaa gaattggaag aacgaaaaga aaagctgatg
    14941 aaggaaggcc tttttcaagg gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg
    15001 gtccttacca gtccaagcgg agcggttatc cgcgatatca tcacgacggt gaaaagaaga
    15061 tatccaattg tccaattggt tttgttccca acagttgtac aaggaaacca agcagcggac
    15121 aatgttgtca ggaatatcca gagagtagaa gagatgggca atttcgatac aatgatcatc
    15181 ggtcgtgggg gtggttcgat tgaggattta tggccgttta atgaagagcg ggttgctcgg
    15241 gcgatttatc aagctaatac gccggtgatt tcatctgtag gacatgaaac agataccacg
    15301 atcgctgatt tagtagcaga tgttcgggct gccactccaa cagcagctgc agaattagct
    15361 gttcctgttt tgtcagaaga gttgatgcgc attcaagaac ggcaagctag attgcagcag
    15421 gcgtttgtac gccagatcca gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat
    15481 gtatttcggc agcctgaaag attgtatgaa ggtcagacca tcaaattaga ccagttgaaa
    15541 cagcgattat atcaagctac acagcaaatt tatcatgcga aagaaaaaca aacgatcgca
    15601 ttaagccacc aattggaaca agtggctccg ctttatcgag ttaaagcagc tcgccaagaa
    15661 actgcttatc tagaaaaacg cttagaagag aaaatccaac aatatatgaa acaacagcag
    15721 cagcgttttg ctcaagctgt tcaatctctt gatttgttga gtccattgaa aatcatggga
    15781 agaggctaca gctatacgac gttaaatgat caagtagtaa aaagtgtcac cgagattcat
    15841 caaggggatt tgctgacgat ccattatcaa gatggaatcg ttaaaggaca agtacaagag
    15901 atagacaaag gagaataaaa atatggaaaa tccaactttt gaagagtcac tgcaagaatt
    15961 agaaaaaatt gtcatgcagt tagaacaagg agacgttcca ttagaatcag ctctagattc
    16021 gtttaaacga ggaatggaat taagcaaaca ttgccaagat acattagcta aagcagaaga
    16081 gaccttgacg aaaatgatga cagaatcaaa tgaagaagtt gattttgacg gaaacgagga
    16141 aagctgagat gagattaact gacttttcag ctattcatct acctgctgta gaaaaagaaa
    16201 tcttgtcttt tttagatgag catacaacgg atagaggact ctatgatgcg atgagttatt
    16261 cagtgaaagc aggcgggaaa agaatacgtc ccttattact tttaacggcc gttgcttcct
    16321 ttgatgagcc tattgatgtt cccgtctatc aagtagcagc tgctttagaa atggtgcata
    16381 cgtattcctt gatccacgat gatttgcctg cgatggacaa tgatgatctt cgtagaggaa
    16441 aacctaccaa tcataaagta ttcggcgaag cattagctat attggccgga gatggtttgc
    16501 ttacaggagc attccagcta atcagtatgg cacacttagg taattctcct aagcttctgc
    16561 tgcttcagca attagctgtt tgtgcgggat cacaaggtat ggttgctggg caagctgcag
    16621 atatcgaggg cgaatcgaaa aagctttctt tagaagaatt agcatttatt catgaaagaa
    16681 agacagggca tttgattcgt tatgccttgc tggctggtgg catattagcg aagcaacctg
    16741 aagaaatctt attgctactt caacgattag ccgaacattt aggtttagct tttcagataa
    16801 gagatgatct actcgatgtg attggtacaa cgaaaacatt agggaaaaca gctggtaaag
    16861 atgaacgtat ggaaaaaaat acttacccgc gtttattagg cctagaaaag acacgtgagg
    16921 cgttggagat tgagctgtta tccgccaaca agatcataga caaattagaa gaggacgttt
    16981 tttcttttga tggcgggctg atgcgccaga tgatcaagca atttgatgtg gagaaatgag
    17041 ggaaagaatg gaaaaagaac gcgtagatgt attagcggtc aaacaaggat tatttgaaac
    17101 aagagaacaa gccaaacgtt cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg
    17161 gtttgataaa ccgggagaaa aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa
    17221 acttccttat gtttctcgag ggggattaaa attagaaaaa gcactcaaag catttgatct
    17281 atctgttgaa ggaaaaacat tgttagatat tggtgcatcc actggcggat ttactgatgc
    17341 agctttgcaa aatggcgcaa aaatgagtta tgctttagac gttggctaca atcagctagc
    17401 ttggaaaata agacaagatc ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc
    17461 aaaacccgaa gactttacct taggaattcc agaagttgct gttatcgatg tttcttttat
    17521 ttcactaaaa ctgatgctgc ctccattaca tgctatttta aaagaagagg gagaagtgat
    17581 tgctttaatc aaaccccaat tcgaagcagg cagagaagct gtagggaaaa acggaattgt
    17641 tcgtgaccct cagacccatc aaaaagtttt agaagacatt ttgtcatttg cggcacaagg
    17701 tggatatgat gtgctggaat taagttattc tccgatcacg gggggagaag gaaatatcga
    17761 gttcttggct cacttgagga aagtacccga aagcggaaca atcaattcag ctattaatat
    17821 ggcagaagtg gtttcaaatg cacacgaaca gtttgaccat aaataaattg taactgtgaa
    17881 aatcaagtga gaagtatgcg gattcttctt tattatacca aggaaataag ctatcattga
    17941 ttaagaagaa tatgctaagg agcgaagacg taatgagaaa aaaagatcgg catcgtttga
    18001 ttactcgatt gttgagtgag caagatatcc gaaagcagga agaatttgtt gaaatcctga
    18061 aaagcaaagg aatatctgtt actcaagcta cgatttccag agatataaag gaactgaaac
    18121 tgatcaaggt accggcttca gatggcggct accgttatag cataccagct gaaacaaacg
    18181 aagatgtaaa tactaaattg gaaaaactat taaaagacgc tttcgtggca gttgaccaaa
    18241 tggaaaaatt tgttattttg aaaacattgc cgggaaatgc ttcggcagca gcaaatttga
    18301 ttgataaacg ttacaagaaa gaattatttt cgattatcaa tgatgacgat aatgttttga
    18361 tgattacacg aactgaagac caggcagttt tattgaagaa agacttcctg cattaccttt
    18421 aagttgagga gcgaatgtga tgctacaaga aatcagtatt actaactttg cgattattcc
    18481 ggaattgcgg ctttcttttc acgaaggaat gaccgcgctg accggagaga ctggtgcggg
    18541 taaatccatt attatcgatg ctttaggtct gttagcagga ggaagaggtt caagcgatta
    18601 tatacgtcaa ggagcagaaa aatgtgtgct tgaagggttg tttgaactac caaaacaaga
    18661 aggattctct gaactgatgg ttgaattagg gatcgaaaca gatgaggaca atctgattgt
    18721 tcgacgggat atgtccttaa ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac
    18781 attagctaat ctgagaagaa tcggcagtta tttagtagat atccaaggac aaaacgaaca
    18841 tcaggaactg ctccagcctg aatcacatct tgctttattg gatcgttttg gagatgtagc
    18901 tttccaacag aagaaaaaga gttatcagca agaatatatc tcttatcgtg agctggaaaa
    18961 aagggtacga aaagtgcagc aaaatgaaaa atcctatgtt cagagaatag atatgctcca
    19021 tttccagcaa gaagaaatag ctgctgctga tttgcaagtc ggagaagaag agaaattaaa
    19081 agaagaaaga gaaaaactta gcaattatca aaaaattgct gatggattag ctgcaggtta
    19141 cggagcactg acgaataatg aacaaaacag tgtagatggt gttggcttgg ccgtttcaga
    19201 actgcaaggg atcgcccatc tagatgtgga gtatgaagca atttatgaaa atatccaaag
    19261 tgcttactat ttgctgcaag atgcaatcgg cgatatgagt cgtcagattg atttattgga
    19321 gttagatgag agtcgtttag aagaagttac gcaacgttta gaattaatcc gacagttgaa
    19381 acgtaaatac ggcgaatcca ttgagtcaat attggcttac tatgacgaaa tcaccgaaga
    19441 gctagcttct tctgattttt cagaaggtca attggacaaa atgaaagagg aactagagca
    19501 aaaagagctt ctacttcagc aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc
    19561 aaaagaattg gaaaaatcca ttctgcatga attgaaaagt ctgtatatgg aaaacactga
    19621 gtttgaggta cgtttcttaa aagaagaaaa tagacaattg aatcatgatg gatttgacca
    19681 aatcgaattt tacattacga ccaatccagg agagccactg aagcctttgg tgaaagtcgc
    19741 ttctggtggc gaactgtctc ggatgttgct tgctttgaaa actatttttt cttctgagca
    19801 aggtgtgaca agtattattt ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca
    19861 ggccatcgca gataaaattt ctcaaatatc gaaatactct caagttttgt gcatcactca
    19921 tctgcctcaa gttgctgcag tcgccgacta tcaatattat attgtgaaag cagtgattga
    19981 tggacgtacg caaacttctg tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc
    20041 acgtatgctg gctggaagtg agattacaaa actcacactc gaacatgcaa aagaactatt
    20101 gaaactcgca aaaaggacct gagccacctc gcagtggttc aggtcctttt cagcagctga
    20161 tagctatatt ttatttttac atgacactca ttacagcagt caatacaaca gatccaatcg
    20221 ttaaaatcag cattgtccaa atgacaaatt ttgttacttt agcaaaagta cttgtttgtt
    20281 ttttttcacg cattgttttc aacctcgtca ttaattagtt gattcaagtg tacaatcttt
    20341 tttccatttc cacaagaggg cttttttata aaaacttccc tttttttaaa taataggtag
    20401 ttacgataca aataaataat gttaatagaa ctagccatat aaaggcatca tccctttcag
    20461 caataggtaa cttaatgttc attccataga tactgccgac gattgtcggt atagttaaaa
    20521 taatcgttaa agaagttaaa attttcataa tattattcag attattcgat acgattgctg
    20581 aaaaagtctg attcatttgt tctactagtt ttaattgaat gcgagaagta gtcattgcct
    20641 gctctgtttc caccaataca tcacgtaaat gatttttcaa agcatgattt tcttggaaaa
    20701 gtttattttg cgctagttct ttcaatgcat ctaaattagc agaagtcgct tctctgaaat
    20761 aaacgaggct tttttggata tccatgattt gataaagttg tttgttttca gtggatactt
    20821 gaagtttggc ttccaaagta tcgcattgag attgaagcgt agctaaataa ctgttgtaag
    20881 agagtgtgac ctgccaaatg aattgaaaga agatattgag attaggcgaa atatcacttt
    20941 ttggaaaggt ctggctaaag actttcttta aaaacagtgg ttcatgattc gtaattgtga
    21001 taagcttctt tttgggcgtc aaaataatag agagcgggta tgtatccact tgaaaataac
    21061 cgctaggact tgttttagca aaaggatatt gtaacaataa taacgctggc tttgtcaact
    21121 ttgattgatt gaattcttct gtacgggaat tttctgcatc gtccaatatg gaagtgatgt
    21181 aatctgacgg aatttcaaat tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt
    21241 ctacacaata ccagatcgtt tggtcagttt cctttccggt attacgcagt ttctcattat
    21301 ctaatgtaaa atatttgatc atgattttcc tcctgttgcc tgtttctttt attataacgg
    21361 atagggaatt ggaatacatg gaatgtgttt gtaagatata gaatataact gcataaattt
    21421 aaataaaatg aaacagttgt gtaaagtata tacagtatac ttgaagcaac gataaaaaag
    21481 gagtttacgt tcatgggcac attcacacca cttatcttgt tttggtatat ttacccggta
    21541 atcgttttgt ttggttgcca atttcttgta aaattatttt cgttaaatcg tcgttttaaa
    21601 ataaaagccc ctgatttggc gattcctttt ttgtggggcg gtatgcatgc attgtctaga
    21661 aatacaggga caatttctat cttaccgttt ttctttattt ctgttttgct aatgggaatt
    21721 tgtattgctg ttttccaagc atattattat gaagaaatta tttatccgcg ttatttcaag
    21781 atgacttggc gtttggtttt tctgttgaca atcgtcttgt acgctgtctt gatcattgtg
    21841 aacatacttt cttacctatg aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt
    21901 tttgcagctt gatttttaaa aaaatgttta tgaataaaaa aagttgttcc cggatttccc
    21961 agaaaaggtg gtagaaagtg ggggattgtg gtagactaat tttagttagt ggaggaatgg
    22021 ggggctttca atgttgatgg gtgaattcca acataatatt gatgcaaaag gacgtttaat
    22081 cgtgccctca aaacttcgtg aagaacttgg cgaaaagttt gttctgacac gcggattgga
    22141 tggctgtctt ttcggctatc cgatgagcga atgggaaaat ctggaagcta aactgaacga
    22201 aatgccgttg gctaaaaaag acgcacgtac ttttgtgcga ttcttctatt cggctgccac
    22261 tgaatgcgaa cttgataaac aaggacgaat caatataccc agcacgttaa gaaattatgc
    22321 agctttgaca aaaggttgcg tagtcatcgg tgtatctaac cgaattgaaa tttgggatga
    22381 gacacgatgg caagattttt ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag
    22441 tatgattgat ttcggactat aagaatagga agaggctgac taatgactga aacatttcaa
    22501 cactatacag tcatgttgaa agaaacggta gatggattga acatcaaacc ggatggcgtt
    22561 tatgtagact gtacactcgg cggtgccgga catagtgaat atttattaag ccaactaagt
    22621 tctaaagggc atctgtatgc gtttgaccaa gatcaaaaag caatcgatca cgccaagctg
    22681 cgtttagctt cttatgtcga acaaggtatg gtcacattta tcaaagcgaa cttcagagaa
    22741 ttagaagaac gcttgagtga gcatgtagat aaggtagacg gcattttgta tgatctaggg
    22801 gtttcctctc ctcagctaga tgaagcagaa cgtggattta gttatcatca agatgcaccg
    22861 ttggatatgc gcatggatca gtcagctcct ttatccgctt atcacgtaat taatgaatat
    22921 tcctaccatg aattagtaaa gattttcttc cgctatggcg aggagaaatt ttccaaacaa
    22981 attgctcgtg aaatcgaacg agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt
    23041 gtcgaaatca ttaaacaagt catcccagct cccgcaagaa gaaaaggcgg gcatcctgca
    23101 aaaagaattt ttcaagctgt gcggattgca gtaaatgacg aactcggtgc cgaagaggcc
    23161 tctttagaac aggcaatccg gttattgaaa gtcaatggaa gaatcagcgt aatcacattc
    23221 cattcattgg aagacagaat tgtcaaaagt atgtttaaag aatatagtac ggtacaagat
    23281 ctaccaccgg gacttcctgt catacctgac gaattccaac cagaactgaa agtcattact
    23341 cgtaaaccaa tccttccttc agaggaagag ctaacagaaa acaatcgatc acgcagtgca
    23401 aagttacgta ttgcagaaaa aataagaatg aaagaggagt agaatgatgg cagaattaaa
    23461 gaaaatgcaa gattacgaat atgatttacc tgaattggtg gatcagccgg aacaacaaat
    23521 gcctgattct tcttcgaatc aagaaatcat tcttccacaa tcaccggcaa aacgattaaa
    23581 gcagatttct gctttggaaa aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt
    23641 atgtgtttta acagttatgc tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc
    23701 aatacaaaca gaaatcaatg ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa
    23761 cgaactttct cgtactgaac ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa
    23821 tgacgacaat ttaaggaaag tgaagtaaat gagtcttaaa aataaaattc ggcggtttat
    23881 ggaaaagaaa aatctaaatc cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc
    23941 tacgagtatt ggattgttct ttttatttgc cgtccgtttc tcgtatatcg ttattggagg
    24001 tcatgttgct ggtacctcat tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt
    24061 agtaaaagca aaaagaggaa cgatttatga tcgtaacggt gtggcgctcg cagaagatgc
    24121 ctcctcttat tcaattaaag cgatactctc aaaaacatat acctctgggg ataaaaaact
    24181 gtatgttgaa gaaaagaatt ttgataaaat agcagaaatc ctgcataaga atctttctat
    24241 agacaaaaaa gatgcactga atattttaga ggacggcgca aaaaaagagc tgtatcaagt
    24301 tgaatttggt agctatggga aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga
    24361 catgaaaaaa gaaggtgtcg ctggtcttta ttttgtcgac catcaggctc ggatgtatcc
    24421 aaatggcgtc ttttcttctc attttattgg atacgcagtt ccagacaaag atgagaatgg
    24481 cttagttggt aaactaggtt tggagtctgc ttacaatgat atcttgagcg gtaaagatgg
    24541 taagattatt tatcaaaaag ataattttca aaatcctctg ccaggtacag tggcagaaga
    24601 agaaaaagct gtcgacggac aagatatcta cacgacttta gacagcagac tacaaagtta
    24661 tttagaaaca ctgatggatc aagtaaatga agaatatcag ccagaagaat tgacagcagt
    24721 cctgatgaaa gcaaaaaccg gagaaatctt ggcaatggga caacgaccta ctttcaatcc
    24781 tgaaacaatg gaaggattga ctggaaaaga tgctatctgg cgtaatttct tggtacaaga
    24841 tagttatgaa ccaggatcaa ccatgaaagt tttcactact gcagctgcta tcgaagaagg
    24901 agaattcaac gagaatgaaa ccttccaatc tggaaaaatc caagtagaag atgctacgat
    24961 caatgaccat gactttggtg aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc
    25021 aagtaacgtc gggatggtca tactggaaca gcgattaggc ggacgatggt ataactattt
    25081 acaaaaactg ggatttggac aaagtactca ttctggatta gatgatgaag taaacggtgc
    25141 tttacctaca ttaaatattg tcgaccgagc aatgagtgct tatggacaag cggtaggtgt
    25201 gacgaatttc caaatgatga aagctttcac ttctattgcg aataacggta caatgattca
    25261 acctcgatat atcagcaaag tggtggatcc ccaaactgga gaagagcgaa caactcaaac
    25321 agaagttcta gggcaacctt tttcaaaaga aacaacagaa aaagtccgtg aatatatgag
    25381 agatgttgtc gaaagcgaaa actacggtag tgcttatggt gtatatagtg tacctggata
    25441 caatgtatcg gcaaaaaacg gaacagccca aatcgcttct gatacagggg gatatcaaac
    25501 aggagataca gcctacctgt attcgattgt ggagatggtt ccttctgaag atccagacta
    25561 tgtcttgtat ctaacaatga aacaccctaa gacatatgat cgaatggcat tggctaaaat
    25621 cgcaaatcca ttgatgaaac gggcaatgga ttttaaagaa actgaagaag attcagatac
    25681 agaaacaaaa acagaaaaag tatccgtagc cgattatcgt aatcttgaag cggacgttgc
    25741 agcagctgat gctcaaaaaa gtggactgca acctgtagtg atcggtaatg gtaaaaaagt
    25801 acaaaaacaa tctactgcca acggtgatca gttgatctct ggagaaaaac tgatcctata
    25861 tactggtggt gataagctga tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa
    25921 gctggggaaa atcctcggta ttgaagttag ttttgatgga gatggttatt gtgtcaaaca
    25981 agaacttgct ccatatgaaa aaatcactga agacaaactt agttttactt tagaagaata
    26041 agaagagaaa taggagagaa aagagcatgg aatggacaca agcattgatt cccattgtca
    26101 gcagttgtgc aatgacaatt gccgcaatgc cgctatttat tggttatttt caaatgaaaa
    26161 agcaaggtca ggcgattcgt gaggagggac caaagtggca caatagcaaa gccggaactc
    26221 ctacaatggg cggcttagtg tttctgatcg gctctatttt gacaggaatt tgggttggag
    26281 cttggcaaaa acagctaact cctactcttt tcatcttgct ttttgtatta gcgttatacg
    26341 gagtaatcgg ctttcttgat gattttataa aaatattcaa gaaaagaaac atgggactga
    26401 actcgaagca gaaattgatt ggacagattc tagggggaat catcttttat cttgtttacc
    26461 gttctgaagg ttatccaggt gtattgaatt tcttcggact agatctacct ttaggaatca
    26521 tttatggtat tttcgctctc ttctggcttg ttggattttc aaatgcagta aatctgacag
    26581 atggcatcga tggattagtt gctggcttag gaagtatctc ttttgcagct tacggtctaa
    26641 ttgcttggca tcagcatcag tatgatgtat taatcatttg cttaagtgtt ttaggaggtt
    26701 tactgggttt cttcgtctac aacagaaaac ctgccaagat ttttatggga gatgtcggtt
    26761 cattagcgtt gggcggactt ttagcggcaa tttctataat gctaaaccaa gaatggacat
    26821 tgcttttgat tggtttgatc tatgttatgg aaacagcaag tgtgatgctt caagttactt
    26881 cctttaagct tacaggaaaa cgaattttta aaatgtctcc aattcatcat cactttgaaa
    26941 tgtgcggatg gtcggagtgg aagattgata cagttttttg gttgatcagc attgtgactt
    27001 cgttgatcac attatggatc gtttggtagg agatgacggg aaatgaaaaa aatatcaaca
    27061 tatgaaaata agaaagtgct tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa
    27121 ttattacatg aattgggcgc acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat
    27181 ccggaagcac aagagctgct ttctttaggg ataaaagtaa taacaggtag tcatccaatc
    27241 gaattgttgg atgaagaatt ttcattgatg gtcaaaaatc cagggattcc ctacagtcat
    27301 cctttagtag caaaagccca agaaatgggt attccggtaa ttacggaagt tgagctagct
    27361 tacgaagtag cggaatgccc aattattggg attacaggaa cgaacggaaa aacaacgaca
    27421 acaacgatga caggactact actgaatgca ggagcagatc aaggaattgc acgtttagcg
    27481 ggtaatattg ggtatccagc aagtggtgta gcgcaagaag caaaatcaga agataaaatt
    27541 gtgatggaat tgtctagctt ccaactcatg ggaatcacag atttccgccc tcatatagcg
    27601 gtgattacca atatctatga agcacatatt gattatcatg gtacaagaaa agaatatgtc
    27661 aaagctaaat ggaaccttca aaaaaacatg acagaaaaag attatctgat ccttaactgg
    27721 aatcagtcag aactacaaga attagcgcaa agaacaaaag cacgcgtact gcctttctcg
    27781 acaaaagaag tgttggaaga cggtgtgtat gctgacgatt actcaatata ctataaaaaa
    27841 gaaaaaataa tggaaatatc tgaacttggc gtaccaggta agcataatgt agaaaatgca
    27901 ctagctgcta tttctgtagc aaaattgtat ggtatatcaa atgaagctat acgagaaacg
    27961 ttgcattttt tccatggcgt accacatcgt acgcaatatg taggagaaat ccaaggcaga
    28021 aaattttata atgattcaaa agcaaccaat attttagcga ctaaaatggc tctaagtgga
    28081 tttgaaacgt ctaaggtcgt gttattggct ggcggattag acagaggaaa tacatttgat
    28141 gaattaattc cttcattaaa aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa
    28201 aagttgatgg atgctggaaa aaaagcaggc atcgagacaa tagtaacagc cgattctgta
    28261 gaacaagcag ttcctttagc attagaaaac agtacagatg gtgatgtagt tttattatca
    28321 ccagcgaatg ccagctggga tcaataccca aattttgaaa ctagaggaaa cagattcatg
    28381 gaagctgtga accgattaaa atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt
    28441 ggttacaggc ggaggaacag ggggacatat ttatccagct cttgcctttg tcaattatgt
    28501 aaagactcaa gaaccgaatg ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa
    28561 caaaatcgtc cctgatacag gaatgccttt ccataccttg gagattcaag gatttaaacg
    28621 aaaaatttcc atgcataaca tcaagacggt ccgattgttt cttaagagta tccgtcaagc
    28681 aaaaaaaatc ctcagtgatt ttcaaccaga tatcgttatc ggtactggag gatatgtttc
    28741 cggagcggta gtgtacgctg cagccaaaat gggtattcca acagttatcc atgaacaaaa
    28801 cagtgtacca ggtgttacca ataaattttt aagccgatat gtggatcgga tcgctctttc
    28861 atttaaagat gcggcttcct tttttcctga aaacaaagca gttcttgtag gtaatcctcg
    28921 agcacaagaa gtagcagata ctaaaaaatc agatattttg aaaacatttg atttggatcc
    28981 agaaaagaaa acagtcttga tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc
    29041 agtatcagtt tttttaagtg aatatattcc ttcttcttac caagttttgt atgcttcagg
    29101 agatagatat tatgaagaga tcctaaataa aataaatcct gaaatacaaa atattagtgt
    29161 ccagccttat atcaaaaata tggcagaggt catggcttcc agtgatctgc tggtggggcg
    29221 tgctggtgca acttcgatcg cagaattcac agctctgggg ttaccggcag tgttggttcc
    29281 cagcccatat gttacgaatg atcatcagac taaaaatgca atgagtcttg ttcacgcagg
    29341 agcagcaaaa atgattgcag ataatgaact aacaggtgaa tcactaagtc agacagtcaa
    29401 cgaaatcatg ggtgatgaag aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg
    29461 aatccctgat gcttcaaaac gtctttatga cttagtaaaa caaataatca aaaaataaag
    29521 gcggtggtct tagtgagtaa gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat
    29581 gaaaacaatt tgactccttg gcaaaaggct aaccgaaaat atctggcaga acaagagatg
    29641 aaaaaccaag agccaaaaaa agaagacata acattagata cacccctttc agattctgaa
    29701 gaagaaaaaa cggttcaaga ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa
    29761 ttttttgaag agatcaagca aaagaaaaac ggtggaccat ataacggttc ttttttgaac
    29821 cggctgccca atttaaaaag tcaacggcac aaagtactat ttcgtcggtt agctttgatc
    29881 atcactgtat tgggtatacc gctgattttt ttgatctatt atgtttctcc ttatagcaaa
    29941 ttacaagcag ttgcagtatc aggcaataag acagtcaaca gccaagaaat catctctgat
    30001 acaaaattgt cattaggaga aaatgtttgg ggacagtatt ttcatcgatc tacttatatc
    30061 gatcgcctta aaaaagcaca gccaagaatc gaaacagcaa atatacattt caaagggatg
    30121 aatgaattcg atttggatgt aacagaatat aaagagattg ctttgatcgc aaaaaacaat
    30181 caatactacc cagtgattga aaatggaaca gtattagatg aaaaagtagc aaatcctaca
    30241 aaaaatctcc ctatcttgga agagttcaaa gactctgcaa aaatcaaaga attaacaaag
    30301 caatacaatc agttatcatc agaattacaa aaagcaattt ctgaaatcaa gtacacgcca
    30361 agagcaagca ataaaaatct gatccaattg aatatgaatg acggcaatca agttattgta
    30421 aatatcaata atcttgcgaa ccaaatgaaa tattattcgc aagtagccaa agatatggat
    30481 gaaaaaggtg tgatcgacat ggaagttggt atcttctcgc atccttacgg tagcactcaa
    30541 gaaacggaag aatcgggcca atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa
    30601 acaaacgaat caagtgaaga ggcagaaacg caaaaagcac aaacacctgg atcgtctagc
    30661 acaaacggat catctgattc aaatgattca agtgaaacaa gtcaaataga agataatcca
    30721 agtacaactc cgtcaagttt aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt
    30781 actttctaaa aaccagtaat ttatggtaga attggaagca gtgaatatat ctattaataa
    30841 gtatatttta aaacggacgc tagttgttcg cttgtatatg gaatagtaag taggagggaa
    30901 tcccattcat ggcaaaaaca ggaatgtatg taggccttga tattggaacg acatctgtta
    30961 aagttgtcgt ggctgaatat atcgacagcc aaatgaatat tattggagta ggaaacgcaa
    31021 aatcagaagg gattaaccga ggcattatcg ttgatatcga taaaacggtc caagcgatac
    31081 aacgagcagt acgacaagca gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg
    31141 gtttgccagc aaacatgctt gaagtagaaa attgccaggg aatgattgca gttaacggag
    31201 attcaaagga aatcaccgat gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt
    31261 caatacctcc tgaacgtcaa atcgtgtcaa tattgccaca agattttact gtcgatgggt
    31321 tcgagggaat caaagatcct cgtggaatga tcggcgtaag attagaaatg tacggtctgt
    31381 tgtttacagg acctaaaacg atcatccata acatacgtaa atgcgtggaa aatgccggat
    31441 taatcgtcaa cgaaatggtg attactcctt tagcgctgac tgaatcaatt ctttctgacg
    31501 gagaaaaaga ttttggtacg atcgtcattg atatgggtgg cggccaaaca acaacggctg
    31561 ttatgcatga taagcaattg aagtttacca atcttgacca agaaggtggc gaatttgtca
    31621 ctaaagatat ctctattgta ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa
    31681 actacgggga tgcataccca gaacggacat caccagatga agaatttcca gtagatgtga
    31741 ttggtcagtc agaaccagtg aaagtcgatg aacgctactt gtcagaaatt atttcagctc
    31801 gaatggaaca gattttcaac aaagcaaaag atgcattgga ccaaatcgaa gcattagagt
    31861 tgccaggtgg cgtcgtattg acaggcggag ctgctagtct tcctggtgtt gtggacttgg
    31921 cacaagaaat cttcggcgta aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa
    31981 acccggtatt tacaaatgta ataagtatcg tggattattc agcaaacttg agtgaagtgt
    32041 atcaattggc aaaaattgct gttacgggtg aaacctcagc agctcgtcaa atggtagttg
    32101 aacaagaaac aacaaacaca tatgagagct atgaagcacc tgaagaaaca atctacgatg
    32161 agccggaacc aaaagagtcc ggagaaaatg taaaaaataa aatcaagggt ttctttacaa
    32221 atatttttga ttaattcatc ctgggaggaa taagatatgg aattctcaat tgataataat
    32281 atcaatgacg gcgcagtaat caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta
    32341 aaccgaatga ttgaagaaaa cgttaaaggt gttgaattca ttacagccaa cacagacgtg
    32401 caagcattaa aaaattcaaa agctgaaaca gttatccaat taggccccaa atatactcgt
    32461 ggtttaggtg ctggttctca gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa
    32521 tccttacgcg aagctttaga cggtgcagat atgattttca tcactgcagg aatgggtgga
    32581 ggaaccggta caggtgctgc accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg
    32641 actgttggtg tcgtgacacg tccattcact tttgaaggac cgaaacgtgg ccgttttgct
    32701 gcagaaggta tcgctcgttt gaaagaaaat gtggatacat tattaattat ctcaaataac
    32761 cgtttattag aagttgtaga taaaaagaca ccgatgcttg aagctttccg tgaagcagat
    32821 aatgttttac gacaaggtgt acaaggtatc tctgatttga tcacagcgcc aggttacgtt
    32881 aaccttgact ttgctgacgt gaaaacagtg atggaaaacc aaggtacagc attgatgggg
    32941 atcggtgtgg ctagtggaga agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct
    33001 ccattgcttg aaacatctat tgatggagct gaacaagtac ttcttaacat cacaggcggt
    33061 ttggatatga cattgttcga agcacaagat gcttcagata tcgtagcaaa tgcagcaact
    33121 ggcgatgtaa atatcattct aggtacatct atcaatgaag aaatgggcga tgaaatccgt
    33181 gtgacggtca tcgcaacagg tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca
    33241 gctagacaag cacaaatgca atcacctgct caaaagactg tattagatat ggatcaagct
    33301 aaaccaactt cttcagaaga agaaaacagt tttggcgatt gggatatcag acgtgaacaa
    33361 aatgttcgtc ctagagttga tgattccaat tttgacaata tcgaaaaaaa agaatttgat
    33421 acattcaacc gcgaagaaac aaaatctcat ggcgatgatg aattaaatac gccaccgttt
    33481 ttccgtcgta aaaaataaag gagaggaaga gcacatgatt gctgacaact tgcagaaaat
    33541 caagcaagaa attcaggatt cgtgtgctct tgttcatcgt gatccgaaag aagttacatt
    33601 agtagctgta acaaaatctg ttgaatccgc tcaagctgaa gaattggtta gagctggcgt
    33661 tatgaattgt gcagaaaacc gagtagataa attattagaa aagaaacaag atttatttga
    33721 ttttcctgaa ataaagtggc atttaattgg taatttacag cgcagaaagg taaaattgat
    33781 tgtaaatgaa attgattact ttcatgcgtt ggacagttta agattagcag aagaaatcca
    33841 gaagcggtta gagaaagaat tatcctgttt tattgaagta aatgtttctg gtgaggaatc
    33901 aaaacatgga atttcaccag atgagctttt accgtttgtg gaatctttag ctgcctgcac
    33961 aaaaatcaag gttgttgggt tgatgactat ggcgcctaaa gaagcttctg atgagcaaat
    34021 ccataaagtg tttggtacgc tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc
    34081 ccatgcacca tgtagtgaat taagtatggg gatgagtcaa gactttcaaa ttgcgattga
    34141 agaaggtgca acatttatcc gtgtaggaac agcgctattc aaagacgaat aaaggaggaa
    34201 caaaaatgcc gcttttaaac agaggagcaa ttgctaattt tttcggatta gctgatgagg
    34261 aagagtacga ataccaagat tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa
    34321 aaaatacaac acagaatcaa tcaactcatt ctgtatctgc tgcgaatact catgaaaaac
    34381 aaaaaacggc gacttcacgt cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa
    34441 aagtaattga actacatcaa tcacctacaa aaactgtagc gaaagaaaga acagcttcaa
    34501 gaacctctac acaacctaaa gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag
    34561 cttattctga agctatggcg attgctaaac gaatcattgc tggagaagca accttagtga
    34621 attttcattt agtagaagaa aaacaagcga gaagaatcgt tgattttcta acaggaacag
    34681 tttatgcact agatggagat atcaaacgtg ttggagatga gatttttctt tgtacaccga
    34741 ctgggatgga gattgacagt tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc
    34801 tgtaagagga ggaactacga tttatactat tgcactatta cttcatttac ttcaacgggc
    34861 agcctatttc tatagcatta tcttgatggt atatgcattg ctctcttggt ttccaggcgg
    34921 gtatcaatca gggctaggac gattattagc taagatctgc gaaccgtata tcagtttgtt
    34981 cgatcgtctg cctttgagga ttggaccctt ggatttcaca attttagggg caatcctgat
    35041 cttgaactta gccgtacaag gtttagcaca gatcgttata tggataactg tccatttagt
    35101 ttattgattg aagcaacaaa atctttattc tcagaataga taggagggga aaattttgga
    35161 tgcaaatgta taccagcact ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga
    35221 ttggattgaa caggttcaag gacaatatgc accctatctg accgaatttc ttgacccaag
    35281 acaatcctat attttagaaa cactgattcg acaaagcagt gatttgcgtt tcatgtttta
    35341 tggcggatat gaacaggcag aaagaaaaag atgtttgatt ttccccgatt attacgaacc
    35401 tgtagaggaa gattttgaaa cagcattgta tgaaatccat tatcctagca aatttgccag
    35461 cctaagtcat gggaaaatcc ttggaacttt agtgggaacg gggatcaaac gctcttactt
    35521 tggcgatatc atctcggacg gtgatcgctg gcaagtattt attgcaaatg aggtggatca
    35581 ttttgttgct gcacaagtaa caaagattgg aaaggttgct gtacgcttgg aaccaatcaa
    35641 atacacagaa atgattatgc cgaaagatag ctggacacaa gaaagaggaa cagttagttc
    35701 catgcgacta gacagtgtga tctctgagat ttataatatt tccagacagc ggtccaagca
    35761 gctgatcgaa gctggtaaag taaaagtgaa ctggacagaa aatacaagac ctgattttgt
    35821 acttgaacta ttagatatta tttcagtcag aggttatggc agagtccaga tccaagaact
    35881 tgaggggaag acgaaaaaag aaaagtacag ggtactgttc ggtgtattac gtaaataaaa
    35941 taaaaaggtg gtttactcat ggctttaact ccattagata tccaaaataa gacattcccg
    36001 acaaaaatgc ggggatataa tcaagacgaa gtagatgatt tcttagattt agttgttcgt
    36061 gactatgagg aattgactca aagaaatcga gaattagaaa aagcagtcaa acattctgaa
    36121 gaaaaacttg agtacttcaa tgaattaaaa gatgctttga accaatcaat catcgtagct
    36181 caagatacgg ctgataaagt aaaaacaagt gcaagcaagg aatccgaagt gatcgttact
    36241 tctgcacaaa acaaagcaga tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta
    36301 acaactgatg cagaagaaaa agcaagaaaa atcttgacag atgcaacaga aaaagcaaga
    36361 cagcttgcga cagaaactga agatcttaag aaaaaaacac gagtattcca tcaacgtatc
    36421 agcttgatgc ttgagtctca attagaacaa gtgaaaagtc cggaatggga tgaaatttta
    36481 caaccattct ccagctacgt gactgacagc catgaagtga tcaaagaagt tttagcaaaa
    36541 cagcttgaca atgaaaatga tactgatgta aactcagaca caggagtcat agaagcacct
    36601 atgacggcat ttgatacaca ggctattgat atctcgatcg tgcctggtga aaatgaaaac
    36661 gagtagaaca gaaaagcact ttgttattct taaagcgagc cgatgagagt ggaagttcgg
    36721 taagacactg catggtgaaa gatcctctac ttgagacctg cttttccgaa attagagtaa
    36781 gaagcagcgg ctgacctcgt tattggtcac agagagtttg cactagatag atgcaactaa
    36841 atttgggtgg taccacggca tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg
    36901 aaaacaaata aaggagtgta tggttgaatg aaaatgaaag aaaccttgca attaggaaaa
    36961 acaaaattcc ctatgcgtgg taatttgcca aaccgagaag gcgagtggca aaaagaatgg
    37021 gaagaaaatc gaatctacca aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt
    37081 cttcatgatg gacctccata tgccaatggt aatatccatt taggacattc attgaataaa
    37141 atcagtaaag acattatcat tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt
    37201 ccaggatggg atacacatgg attgccgatt gaacaagtat tggctaataa aggcgtgaaa
    37261 cgtaaagaaa tgtccatggc agaatacttg gaaaaatgtc gtgaatatgc actttcccaa
    37321 gtagataagc aacgagcaga tttcaaacgc ctaggagttg caggagattg ggaacatcct
    37381 tatgttacat tagatccttc ttatgaagct gcacaagtac gcgtatttgg taaaatggct
    37441 gaaaaaggct atatttacaa aggattgaaa ccaatttact ggtctccgtc aagtgagtct
    37501 tctttagcgg aagctgaaat tgaatataaa gatgtaaaat ctccatctat ttttgtagct
    37561 ttcaatgtca aagacggcaa agatattctt gatgaagata cagcttttgt catttggacg
    37621 acaacaccat ggacattgcc agcaaaccaa ggtattgctg taaatccaaa atttacgtat
    37681 gtattggtgg aagccgatgg ccgtaagttt gtcgtagcga aagatttgct agaaacagtc
    37741 caatcagaaa tcggttggga aaatgtaaca gttctaaaag aatttgctgg acaagaaatg
    37801 gaatatatga ctgcaagtca tccattctat gaacgtgaat cacttgtgat tttaggcgat
    37861 catgttactt tagacgctgg tactggatta gtccatactg ctccaggaca tggggaagat
    37921 gactatatcg ctggtagcaa atataaacta tctgtagtgt cacctgtaga ctcaaaagga
    37981 ttgtttacag aagaagcacc tggatttgaa ggtatctttt atgacaaagc aaatcctatg
    38041 atcactgatc tattaaaaga aaaaggagct ttgctgaagc tagatttctt tactcatagt
    38101 tatcctcacg attggcggac gaaaaagcct gtgatctatc gggcaactcc acaatggttt
    38161 gcatctatcg ataaattccg tcagaatatt ttggatgaag tagaaaaagt agagtgggtt
    38221 atcccatggg gtaaaacacg tttgtataat atgatccgtg accgcggcga ttgggtaatt
    38281 tcccgtcagc gtgcatgggg agtaccactt ccgattttct atgctgaaaa tggagaagca
    38341 atcatcacac cagaaacaat cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt
    38401 atctggtttg aaaaagaagc gaaagaactt cttcctgaag gatttaccca cccaggttct
    38461 cctaatggcg aatttacaaa agaaaaagat atcatggatg tctggtttga ttctggttct
    38521 tctcatgaag ctgttttacg tcagcgacca gaattatcat tcccagcaga tatgtattta
    38581 gaaggatcag accaatatcg tggctggttc aactcaagta tcacgactag tgtggctatc
    38641 aatggtgtag caccatacaa atcagttatt tctcaaggat ttgctttaga tggtgaaggc
    38701 cgtaagatga gtaaatcttt aggaaacatc attgcaccag aaaaagtgat caaacaattt
    38761 ggtgcagata tccttcgctt atgggtatct agtgtagatt atgaagcaga tgttcgtgtt
    38821 tctatggata tcttaagtca agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt
    38881 ttcttattag cgaatacaga agatttcgat cctaaaaaag atactgtttc gtacaatgat
    38941 ttacgttctg tagacaaata tatgaccgtt cgattgaacc aagtaatcaa agaaatccga
    39001 gaagaagggt acgacaaata taacttcatg catatttacc gtacagttat gaacttcttg
    39061 acggttgatc tatcgtcatt ctacctggat tttgctaaag atgtagttta tattgaagca
    39121 gaagatgatt atcaacgtcg ttgcatgcaa actgttttct atcaaacagc tgtagctttg
    39181 acaaaattat tgactcctat cattccgcat acagcagaag aaatctggtc attcctgaaa
    39241 gaagaagagg agtacgttca gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa
    39301 gaagaattga tggatacctg gactgctttc atggacttcc gtgataatgt cttaaaagca
    39361 ttggaagaag caagaaacag caaattgatt ggtaaatctt tggaagcaaa actaactgtt
    39421 tatccaaaac aacaagttcg tgaaatgtta aaagctttgg atgcagatat tgcacaatta
    39481 ttgatcgttt caccagacta ctttgaagtt atggcagcag atgaacaagt gccagataac
    39541 gctatggtat ttgatgatgt tgctattaca gtggaaaaag cagacgggga aacttgcgat
    39601 cgctgtcgcc aagttcgtaa agatgtagga gtagatgaaa aacttcctca tctatgcggt
    39661 cgttgtgcaa aaatcgttga agacttctat cctgaagctg tagctgaagg attcgaagaa
    39721 aagtaaaagg gaaaaggggg tgtgacatgt tctttgtcac accccctttt gttatcaatc
    39781 gtttaatttt ccacgttcac gataccagtt atctggttgc cagatccatt cgaatccgtc
    39841 tttttcaagt agttcgaaag ctgctttagg tcccattgat ccagctttgt agttgggaaa
    39901 atccggttgt gtttggtccc aagcttttcg aatgacatcc acgatatgcc aagattgagc
    39961 gacttcttcc caatgagaga agttcgttcc atcaccattc aacgcatcta atagtaattt
    40021 ttcgtaagct tctgggctat tttcaaccat ttctgcacta tttctaaatt ccaatttgat
    40081 tggttccgtt tcaaatcctt gccctacttc tttcccattc aaggataaag aaaatccttc
    40141 agttggctga atatagatcg ttagaacatt aggtggaagt gtcgtatcgt cacatggttc
    40201 atcgacagat gttttgaata cattaacagg aacttgtttg aatacgatgt tgatccgagt
    40261 ccctttttca gtcaaacgtt tgcctgtacg tacatagaaa ggaacacctg accaacggaa
    40321 gttatcgata gtgaatttgc ctgcgacgaa tgtttcagtc tctgattgag catccacttt
    40381 gtcttcttcg cgatagccct tgaattgtac accatccaat tcaccttgag catattgccc
    40441 gcgaacgaag ttttctaaca cttcttcttc gttataaatg cggatagctt tcaatgcttt
    40501 gatcttttct gtacggattt ctttttcgga aaatgctact ggcggttcca ttgcaagtaa
    40561 tgctacaacc tgtaaaatat gattttgcac catgtcttta agtgcgccac tatgatcgta
    40621 gtagcccccg cgatcttcta caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat
    40681 atagcgattg ttccattgag cctcaaaaat attattggca aaacggatag ctgagatgtt
    40741 ttggatcatt tcttttccta aataatgatc aatcctgaaa atatcttgtt ctgggaatac
    40801 ttctctgatt tgctggttca attcaaaagc agactggtaa tcagaaccga acggtttttc
    40861 tatgatcaaa cgattgaagc cattttttgt cataatattt tgtgatttca aatgagaaac
    40921 gatcgttcca aaaaattgag gtgccatagc caaataatag acacggttac cgtttaattg
    40981 atagtgtcca tctaatcggt cagacaattc ttttaatgtc tgataatgcg ttgaatcatt
    41041 tacatcatgg gattgatagt aaaaatggct tgcaaaggta gttgcttctt catctgaagg
    41101 attcaatgca cggattgttt ctttgacaac ttcacggtag tgctcatcgc tccagggacg
    41161 gcgagcggtt ccgattacag caaaatgctc gcctaaattt ccttttctgt aaaggcggaa
    41221 caaagacggg tacaatttac gctgggctag gtcacctgta ccgccgaata tcgtaaacaa
    41281 tacatttttt tcattagaca tgtggataca ctcctttata ttttatcgac aaaaatcgtg
    41341 ctggccgcct taaagctgac ggggattttc tttctatcat tttggatagt gatcggtcct
    41401 tcataagctg cgatgtcaac gatctcatat tcttcgttga tattcaggtc aatagtgact
    41461 aagtaatcca acaattcttt ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca
    41521 ggataggact ccaatgtttg tcggcgttct tcatggacaa cttcctcttt tttaggaatc
    41581 gatcctccat gaggacaatg ttcaggatga ttcaaatagt tttctaaatg atctgcaaga
    41641 tgttccgaag tcacatgttc caatacttca gcatcatcat ggacttcatt ccagctatag
    41701 tctaaatgtt caactaaaaa aacttcccat aagcgatgtt tgcgaatcag actgctagct
    41761 ttttgcagtc cgcttgcagt caattgtaca ccttgataag gggaatgttc tacaagctct
    41821 tctttcacaa gtttagaaat catttcactg acagatgcag ctgagacatc tagactagat
    41881 acgatttgtt tattgttgac tttcgttgta tctccaccta gttctaaaat gattttcaaa
    41941 taatcttctc gatttggtgt catattcgat catcctttca tggtaaaaaa ccatcattag
    42001 taagtgtaac aaaaaaaacg ttaattgact atttttctaa ataaaaaaca aaggattttt
    42061 agacgttttt acaacaaaaa acgaaagatg atagagaaga ctcattcttt cgtttataaa
    42121 ggatatatat atacattggg agggatctat ttttcagata acaacttata tgaagtgtct
    42181 gcagattcgt cataaagagt ttgttcggga taagaaaaga taagataaaa accatactga
    42241 tcacgtacga ctaaacgata ttcatctatt tctaccacag atgtgccaag agactgaccg
    42301 ttgatcaaaa acgtgtaatc agatgtaatc tgtaattgtt gtttttgctg gctatcacta
    42361 aagaaccatg taccttgata ctttgagagt ccttctgact tttccgcttg ttttttcttt
    42421 ttcaagtaaa taaaacttga gagaacggta atgagtgaac caagaaaata gatcattttt
    42481 tttatcacca aatcactcct ttatgagaaa ataatcgcat agacatattc tttcttcttt
    42541 atttcattgt accgaataaa agctaaaatt tctctaagac tttcgactgg tttttttact
    42601 gtttttctac aaattatgat aggatacata gtaggaagaa aggtgacata atttgaagta
    42661 tcagcaagtg gaaatagctt tttttattga ccgcccgaat cggtttatag cacattgtat
    42721 gaaaaaagac ggagaaataa ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt
    42781 cctgatacca ggcgctgaag tggctattgt atttgctcct ggacctaaac ggaaaaccgc
    42841 ctatgatctg attgctgtaa aaaagaaggg tgactggttt aatatcgata gtcaattgcc
    42901 taatcggctg gcaattgatg gtattttaga cggcacgatc cttttaccag gattaactgg
    42961 tccatttacg gtatacaagc gtgaagtaac ttatcttcac tccaaatttg atatttattt
    43021 agagacggtg gtcggacaaa aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa
    43081 agcgattggt gcttttcctg atgcgcccac actcagagga ttgaaacata taggagaatt
    43141 aacgtatgcc gcacaagatg gttatgccgc ttatgtcttg tttattgctc agttcgaaca
    43201 tcttcatctg gcaacgatcc atgaagaaat gcaaccagca ctagctgata tggttcgaca
    43261 tgcgcagcaa agcggagttc aaatcctcgc ctataattgt caagtatcgg cagatgaggt
    43321 aactgtcaag caggccattc catttgatgt agatatccct tttgaagatc ctatacaata
    43381 gaaagaaggt aaaataatga tcaacttagg tattatcgga acaaattgga ttactcatca
    43441 atttgtccaa gcagctttaa gcacaaaaaa atatgaattg acagcggtat attctagaca
    43501 tttggaaaaa gcacaggaat ttggttctca ttatgaaggg gatatcgaat tcgcaacaga
    43561 tttgaagact tttttcaata ttactcatat ggacaccatt tatattgctt ctccgaatag
    43621 tcttcacttt agtcaagcta agcaagcaat tttggcagga aaaaatgtga tagttgaaaa
    43681 gccagctttt tcgacgccaa aagaaatgga tgaaatcatc catttggcaa atgaacacaa
    43741 tgtgtatttt tttgaagcag caagaaatat ccatgaatca ggttttaaaa agattgccga
    43801 ttacttacca ttaaaagacg agataatcgg tgcaaatttc agctatatga aatattcttc
    43861 gcgctatgac caagtattag atggagaaga accgaatatc ttctcgactc atttctcagg
    43921 tggtgcattg atggatttag gtgtttacct tgtatatgca gctgttgctt ggtttggaat
    43981 gccaaaagaa gtccattatt tcccaagaaa gatttctact ggtgtagatg gtcttggatg
    44041 ggggatctta cgttatgaga attttgatgt atcgatccag ccaggtaaaa tcggtgattc
    44101 ttatttaccg tcagagattt attttgactc aggaacgcta gtgatgaatg gagtcaatgc
    44161 aattgaacaa gcacaatttc atgatcgcgc gcataaagaa atcattgatt tggcggtaac
    44221 agctgcagaa aatccaatgg cagatgaagc agaggatttt gccaatgtca tggctcatcc
    44281 gaaagatcca gcttggggat tgttgtatga agagtggatc gagctagcta gaaatgtgaa
    44341 ccaagtgatt tatgatttaa gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga
    44401 agatttttaa tgaacgaatt tgtacaagta tttcccgaaa attggcagaa aaaatatgag
    44461 gataaagggt ttatctcacc ttctcttatt caacgagcag tttttcagcc actaagtaat
    44521 aaacaaagta tagtagcggt ttctcctaca ggttcaggta aaacactggc ttatctttgg
    44581 cctctactac taaacgtgga accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct
    44641 caagaactag ctattcaagt agcagatgtt gcacgtgaat ggggaaaaga caaagagttg
    44701 aaagtccaat ctttggtggg cggagccaac gtcaaacgtc agattgaggg tttgaaggaa
    44761 aaaccagaaa tcttgattgg tacaccagga agaatattgg aattgatgaa agcaaaaaaa
    44821 ataaaagcac atcaagtgaa aacaatggta tttgatgaag cagaccagct atttgacgct
    44881 gggaacagcc aaatcattga tcaaattctt catcaagctc caacagagta tcagctagct
    44941 tttttttcag cgacagcaga tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg
    45001 tttgagacaa ttgacgtgac agcagaggac gattctcgaa aaggactgag acattacttt
    45061 ttacgtgtac cgatacgaaa gaaagatgac tacttgagac gtctcacgca tatagaagat
    45121 tttcatggtc tgattttctt taatcaactg actgaattag gcgtaatgga agaaaaattg
    45181 ctgtatagag gtgtaccggt aggaagtctg gcctctgatc agaacaagct actccgaaaa
    45241 gcagcgctag aacaattcaa aaagaaaatg gtcagagcac ttttgaccac agatgtggcg
    45301 gctagaggtt tagatattac aggtcttcct tatgtagtga atgcagaagt tccgttaagt
    45361 gaagaagctt atttgcatcg ttcgggaaga actggtcgaa tgggtaatga aggaatagta
    45421 attacactcg tacaagataa taatctaaga gatttaaaac ggcttttacg tccatcaaat
    45481 ataacacttg aagagatttt tttatacgga ggtaagctgc aaactgagca gccaaccaaa
    45541 gaagatgcag ctcctgcagt aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag
    45601 aacccagaat ataacaaaga aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag
    45661 agaaataaag gagcaaggcg caaataagcg cctttttatg ctatgattat gaaaacggga
    45721 acaaaattgg ttgccagagg aggggttatg tgaaagaacg tcaagaagag atcttgcagc
    45781 tattgacaga aaaccaacaa ggattgacag caagtgatgt agctgagcgc ttaactatcg
    45841 atcgaagcaa tgcaagccga tatttaagtg aactatacaa ggcccatcat attgtaaaaa
    45901 ccgctggccg accagtcgtt tactctttgc caacggaaaa gtcaaaatct gatgaagtgc
    45961 atgtcgactc ttctacgcaa gtgacttttg aaacactcgt tggagaaaat gattctttaa
    46021 aagtcagcat acagcaggca aaagctgcga ttctttatcc accgcggggg ttgcatacga
    46081 ttatttttgg agaaactggt acagggaagt cgatgtttgc tgaatgtatg tatcattttg
    46141 caatcgattc agagatgctt tctgcagatg cgccatttgt ttctttcaat tgtgcggatt
    46201 atgatcaaaa tccgcaactg ctctttggac acatctttgg gatcaaaaaa ggggcttaca
    46261 caggagcagc tcaagatagt cccggtctaa tagccaaagc agacggcggt atattgtttt
    46321 tagatgagat ccaccgttta ccgccggaag ggcaggaaat gctgtttact ttcattgata
    46381 aaggtattta ccgtccgctt ggagaaagtg cacaagtaca tgaagcatct gttcagatca
    46441 ttggggcgac gactgaatct tcagaaagtt tcttatctac gttcaatcga cggattccta
    46501 tggcaatcac cttgccgaat cttgcttcac gttcactgga tgagcgttat gaaatcatct
    46561 ctctttttat caaacaagaa gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag
    46621 cgattcttgc cttcatgctt tatgatgcgg aaggaaatat cggtcaagtg aaacgagact
    46681 tgaaactagt ctgtgcaaaa tcttttttac attaccgtac tcatcatgag aaaaagctaa
    46741 tcattcgaaa agaagaactt tcccttcaag tacaaaaagg attactgaaa atcaaggaaa
    46801 taccggaacg tctcgaccga ttcatggaca gcaaaagtca gtatttgaca tttgaaccag
    46861 gatttgctga tgttgtctgg tcacaagatc cagaacgaaa catgcaggtc tataacgata
    46921 ttgaggaaaa agtcacttct ctttccgaaa ccggtgtgga gaacattgat ctagagaccc
    46981 tcatttcaaa agatgtggat gcttatttcc agacatatgt caaagaattg actaaaagtt
    47041 cgattcccaa agatcttttg ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg
    47101 tggctgaaaa acggttagac cgtatttata atgaaaaagc tcgttttgct tttgctttgc
    47161 atctgcaaag tacattggat cgtgtgagag aaggtcatat gatcgtccac ccagatttga
    47221 ataacgtacg gaaaaatttt aagagggaat tccaggtagc aatcgatctt tcaacgatca
    47281 ttgaagaaga agaacaagtt gagattcctt ttgatgaaat cggctttatt agtatgtttc
    47341 tttctatcaa tctaggagaa agtgagcctg ttaaagaaaa caaagtagat gttctcatat
    47401 tgatgcacgg cagacagact gcttcaagca tgttggagat ggcacaagaa ctattaggaa
    47461 caaaagtagg gaaagctttt aatatgccac tggttatgga agttcaaaca atgtatgaag
    47521 aagtgttaca atatgtcaaa aactatcaag agcatttgac gaatggccta ctcttgttaa
    47581 cagatatggg atcattaaac accttcagtg atttgattta tgaagagact ggcattcgga
    47641 cgaaagctat cacgatgaca agtaccatga ttgtgataga agcgatacgg atggcaagtg
    47701 ttggaagaag cttagaagat atttatcaaa atatccagct ttcctttgaa tcgatcgtac
    47761 gtgatcagtt caaagcggaa aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga
    47821 catgctttac tggtgaagga gtagctgcca aattatatca gcgtattatg ccagtgatta
    47881 atcaaaataa ggtagaactg atccagatgc agtttttaga acgtgaatca ttcaaaaaac
    47941 acatcgatgc cttattggaa gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg
    48001 attatcaaaa tatcccattt ttttcagcct atgacgtttt tgatgatgaa aaactaaata
    48061 ttttaaagcg tatagctgat gatgagatcc cgacagctaa aattgtcgat tcacttgctg
    48121 gatcattaag cgatgtcttt tctattgatg aactgatccg ttcattgcaa aagacagttc
    48181 agcagatcca gaccgatttg tcgattatcg tggaacctgg agtagatact ggaatcgtta
    48241 tccatttagc cttcatgtta gacggattga tcaaacaaga aagccatcgg caatttgaga
    48301 aactgtctat tttctcaaaa atttatcgtt tggaaatgga tatcgttcgg gcaaacttga
    48361 tggcactaga acgaaaatat caggtaaaaa ttccagaaga tgatatcgca tttcttacac
    48421 aaatgtttct tgaaaataaa ataaaaaaat gaacacacta atcatactgt ttacacacta
    48481 aacatctgtg tgcgcactat aaaaaagagc taaaaagcat gaaaacgcta tttcagctct
    48541 tttttttatt ggcatgcttc ttgcgttata taaaagtgta aaaaaataaa aggagtgtta
    48601 aaaatggcaa aaaaaacaat catgttagtt tgttcagcag ggatgagtac gagtttattg
    48661 gtcacaaaaa tgcaaaaagc agcagaagaa aaagggatgg aagccgatat ttttgcggtc
    48721 tctgcctcag atgcagataa taatttagaa tctaagaatg tcgatgtatt attattaggt
    48781 cctcaagtac gtttcatgaa agctcaattt gaacaaaaac ttgcgcctaa aggtattcca
    48841 ttagatgtca tcaatatgca agactatggc atgatgaacg gagaaaaagt tcttgcacaa
    48901 gcagaaaatc taatgaaata aatcaagtat gcagttgggg gtataacaac gtggaagatc
    48961 aacaaaatct agaagccatc atggggttaa tcatgtttgg cggtaatgca aagagcgatg
    49021 caatggaagc gattgcggct gctaaaatcg gtgattttga gttagcggat aggaaaatcg
    49081 ctgatgcaga agaatcactt atacaagcgc atcatgcaca aacagacatg ttgacacaag
    49141 aagcacaagg aaatcatatg caagtgacat tattgactgt tcacagtcaa gatcacttaa
    49201 tgacatctat tgcgtttact gatttagcaa aagaaatcat cgaactttat cgtcgcgtat
    49261 acagcgaata aatacaactt cttttttgaa cggaagaaga aaaatttttg aacggatttt
    49321 ttattttgtt ataaggagaa ctctctttat ataaaaaact tataggagga agtaaaaaat
    49381 gaacggacta tcaagttgga tggagaaata catcctgccg gttgccgcaa aaatcggagc
    49441 gcaaaaacat ttagttgctt tgcgtgatgc ttttatcggc atactgcccg caacaatggc
    49501 gggatcagtc gctgtaatga tcaatgcaat tattcgtgat ttaccgcaac agtttaacag
    49561 tacctatgct gacagtttag cagcagacaa aggaatcttt gccgtattga atgtcatcat
    49621 cggaatcaat ggttttgtct ggaacggtac actagcaatc gccggattga tctttgcctt
    49681 ttcttggggg tataatcttg cccgagctta tggagtaaat gatctagctg gggggatcgt
    49741 tggtttagct accttgattc aaggtatttc ctttggtcca gcattatcag cagcattaga
    49801 tgtgaatgtt ccttcaaatg tcgcagatgc aatcaatagt tctgagattg gtgcaacgat
    49861 tgcagatggc acactgtcag ttggattatg gggttggcta aaactagatc atttgaatgg
    49921 aaacgcttat tttacagtta tgatcatggg agctatttca gtcattattt tttgtaaatt
    49981 gatgcttgcc aacattacga tcaaaatgcc tgattcagta ccaccagcag tatctaaagc
    50041 atttgcagcc attttaccag cgacgatcgc tttatacgtc attgctatta tcaattatat
    50101 cgtaggtcgt gtagctgatg ggcaactgat cattgatttg gtgcaaaaat atattgcaga
    50161 accattccta ggattatctc aagggttggg agcagtattg atcgtcacag tatttgttca
    50221 agtattctgg ttctttggta ttcatggatc aaatgtattg gcaccagtgc tagagggaat
    50281 ctggggacaa gcacaactaa tcaatattga tattttccaa aaaggttata acggaaaaac
    50341 tggtacacca gccgtgttag atgcaatcga agatggaaaa gcttatatgt gggtccgcgg
    50401 atcgttcgat gcttttgcat ggtttggcgg atcaggcgga acgatcgtct tactgatagc
    50461 tatcttgatt ttctctaaac gtgcagatta tctaacagta gctaaattat ccattgggcc
    50521 tggtatcttc aatatcaacg aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat
    50581 catgtttatc ccattcttga ttgctccagt agtagcaaca gcaattggat ggacagctac
    50641 ttatcttggc ctagtcgctc cggtatcgca gcaagtcaca tgggtagtac cgccgttctt
    50701 attgtcattc ttagcaacag gagcggattg gcgggcaccg atcgtaacat tagtatgtat
    50761 ggtcgtgact ttcttgattt gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc
    50821 cgcaggcgaa caacaataag atgaacgtga ggagcgggac gctttaagtt tcgttcctca
    50881 taatgaattt tcgaggagga actgaaatga ttacaattgc cagtgcaatg caacaagatg
    50941 aaatcaaaga attgttagaa ggatacaatg aagaaggaat gacttttaca tttaaagaaa
    51001 aacaaggaat caagctgttc tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa
    51061 aagcaaagga actaatcaaa gcacagcctt ggggaacagt cctttatttt caatcaacag
    51121 ctgtttagaa aatggtttaa aaagatagga tggttgagat gataaaaaac ggtttatttt
    51181 tatgtagtat tggttctttg atcgtgttgt atagtttgaa tccacattcg caaaggtatg
    51241 atttgtatag tatggtcaca ggtctttttc taacaggatt aggaatctat ttcttcatga
    51301 aggggaaaaa aagagaagaa gcaaaggaga aaaataacta atggaaatca cgaaaaaatt
    51361 aaatattcca gcatcgtttt tttacgataa agttatggac tctgtccttt tcgatgtacg
    51421 taaagcgacc ggcaaatcag taactagaaa acagctgaaa aatttggagt acgtcaaaca
    51481 attctcgcaa aataaccggg ctcgcataaa aatcgaagaa gtcattgaaa accagtctta
    51541 taagtttcgt acatcgacaa cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt
    51601 ggacgattcg acctgtgagg ttcattacac agaaaaaatg gagtcttatg gcatgcttca
    51661 aaagttgaat gatatgcttt taggaacaat cttgatgtat tttaaacgga gacgtttcaa
    51721 aaaaatgcta gatatgatcg aagcaacgta ttaaatcaat gaaacatggt actatttagt
    51781 tattgaaaaa tgaagtaagc tagaaggaat ttttcatagg aagatagatc tttcctgcga
    51841 atcctcacta gcttttttgt aggaaggggc agcaaaatga caaacaaagt aagagagcta
    51901 ggtgtatctt cgattgagga aatgtttgat ttagccactt atgcatttaa tggggcagat
    51961 acacctgaaa ggcgagaacg ttttcgtaca cttgcggaga attcttggaa ttacggattt
    52021 tttgatgagg aggatcggct aacaagtcaa gtcatggcga ccccttttcc agtgaatttt
    52081 tacggacaag agtacctaat ggccggcatt ggctatgttg cttcttatcc agaagcaagg
    52141 ggacaaggcg ggatcaatca gatcatggaa aagatccttg aagattgccg tgatcgtaaa
    52201 gtaagcttat cttatctggc gccattctcc tatcccttct atcggcgcta tggctatgaa
    52261 caatcatttg ataaaatcag ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa
    52321 aaatctggaa aaataaagcg tatggatttt gaagatgcaa aagctgcaat cagaagaatt
    52381 tatgaacaga tgccggaaaa ccaacgagct ggtctgaagc gcgaagactg gtggtatacg
    52441 tataaattca agcaaaaagg caattaccag tttgctttgc attttgatga taatgaggaa
    52501 gcaacgggat atatcgttta tcagcttgca acgtcttcct ttattattgc ggagtgggga
    52561 tttttggatc atgatgcttt ttgcagcctt gttcgcttcg taagttctca caatggtgct
    52621 tttgaaacat ttgaatattc atgcggctac ggcggtaatg atcaaaatta tctattagaa
    52681 aatccatttg cttctgtaat gatcacacct tatatgatgg cgaggatcgt agatatttct
    52741 ttgtttttgg aaaattatcc atttaaaaag agacaagctg catttgccct acaaatagaa
    52801 gaagatcatt atgcgaaatg gaatgaagga atcgtggaag taacaatcga aaatggaaaa
    52861 aatcatctct caaaagtaga acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat
    52921 atcacgcagc tattattagg gtatcgtaaa gtggaagagt tggtattcca agaaagaata
    52981 caggtgacaa aagaatatga agaaacgtta agctcaatct tgccacagca acagccaatt
    53041 cttaatgact atttttgata aaaaacgggt gatgacaata tttttgtcac cacccttttg
    53101 tttttcattt ggcatttgaa ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga
    53161 attttttgtt ttcttttgtt ttagggtttt cttttttgtg gtcttggatt gcttctttcg
    53221 taatcttttc ccgatttttt ttagatgaat ctaagcgttc tgcgtatact ttttcttttg
    53281 gcttatcatt cattggaaag gacctcccta cattatttca ttctacctct agtttatcat
    53341 ctgttaagga ctagtggaaa cgttttgcct aacttataag ctgatttttt tagcagataa
    53401 gcttgcaagt aaaaaacaaa caagctacaa ttgatttatt gaaattgttc ctgtagctca
    53461 gtaggataga gcaatcgcct cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg
    53521 tataaataac ccctgtatcc tttgcggtac aggggtttgt tttgtatttg tgccaaaaat
    53581 gtgccaaact agttatcatt tttatataaa ctatcgaaca cagtattgat tttttcttta
    53641 tcctgatctt caagctctcg aacaatatga agataagtag acattgtagt ttctaaactg
    53701 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
    53761 gcatgtgtat gccttagtcc atgcaaagta aattgctttt caatccctaa ttcttttaat
    53821 tttttgcgca aatatttgct gacggcgttt gatgaaacta atccattttt taagttgaaa
    53881 aaaacaaaat tatgagggtt tttgatttca aaattctcga ataattcttt ttgattgatt
    53941 ttaaactttt ttaagagatc aatcgtatgt ttatcaatgg aaattttcct atttgaagtt
    54001 tcgttttttg tttttcccca ttcatttaat ttataattcc atgttttatt aatagtgatg
    54061 atttgatctt cgaaatcgat atcttcccat gttaatccta gaagttcagc aaatcgcatt
    54121 ccagtggcac cagcaactaa cacgagcata ggagaagagt actgcgcaga aaggttttct
    54181 tctacgagtt tcattaaaga tttaaattga tcataatcta aatatttatc ttcttttggc
    54241 ttcaatgaat cttttccttt gattactgct tttctagttg gatcaaacgg aattaatcct
    54301 tcttctacgg catctttcaa cgacgctcta atatggttat taaacttaat aacggtagat
    54361 tttacatggt ctttagcata cttatttaaa aagcgttgat atcccactct atctaaatca
    54421 gaaatcaata ccgctggcat atatttcttt atgttcatta acgtatcttc gtattttcta
    54481 taagtgatag gagatactgt tccttcttta taaagttgca tccaatcctc aaagtaatct
    54541 gataataata gatttttgcg atccccttta aggcctttgt tcaagttata ttctaattcg
    54601 ttggcggcat cttttgcttc tccttttgtc ttaaatcctg attttcttat cttcgtgtat
    54661 tttccgtcat cttttttgta tgaaatttcg tattgccaac tatttccacg tttgactaat
    54721 cttgccataa ttgaatcagc tctctttctc tgatacaata ggaactataa agaagcccat
    54781 tgtttgggtt ttattatttt ttgcacatcc tttttgcttg ccggcgcaag gatgtgctat
    54841 ttgtcgtttt aatggtataa ttggatgtgt attagatcat ccaatcagag gtgtttatta
    54901 tgattaaagt gatttttaaa agaataactg aacttccaga gtttagatta tttgttgatt
    54961 attggtatgt ctggtttttt attgttgcat tgctgatagg tctagatgtc atattagaaa
    55021 gaaaacttaa gtaagagttg agttaataaa ttgaattcct aaatgagata ttttgtagcc
    55081 catattttgt ttgccgacat cacctaatac tatggagcta atcgtttttt ctaacagacc
    55141 aaaatcagat aatctattta agctaccatc tattagctct tgtgaatagc cagacatgct
    55201 gatatttcca gtgaaatttt cgtttccacg actcattaag catgctaaca attgaatctc
    55261 aatcgatgtt aattgattca gtacaagaag ataatactcc tcgttatccc atgaagcgtt
    55321 gttaattcga agtaaacaat tcttataaag atttttatac atatctattt tattttgtgc
    55381 ctttgatttt tctacctcgt catttatccc ttcgaatatt ccaatgagtt ggtccttgtt
    55441 ctctaagttg aaaacatctt ctggaatttg atcgcgaatg ttttctaatg attcgtttaa
    55501 atttttgtag aatttctcga tgcgtttgaa acgcttctcg tttttagacc caaaataaag
    55561 tgactgcaca acgccaccaa tataaggaat tgctccaata ccgccttcaa ctattaattc
    55621 aagatattct tttttggtta gtttttcatt gctattatcc atagttacct cctattttgt
    55681 catttccttt acaagcattg tgactcctga tggcactctt ttgtataggt tttattattg
    55741 tgcacaatct aactcttagt ggggcgtggt tcatttattt tttacttact ttactattaa
    55801 tcatatccca ttgagcatag gatttttcta tgttcgatcc tcttgaacta atttctcctg
    55861 taaaagttac tttatcgccc tttgtaaagg tatcagcatt gtttaagtcc ttcgcaaaaa
    55921 ttacatatga aactttaggt gttgcagaaa tatcattcca acttttatca tcgtactctt
    55981 tgtctgctat aatggctatt ctagtagtca ttgattcgat aacagttcct tcaaaagtat
    56041 atgttttacc actaatatat ttagtaaaag ctgtattttg ctcagtagct ggagtaatag
    56101 aataataact atctaagaaa gcaccccagt catgattatt tgtctctaaa aattcttcaa
    56161 attgagttag gcttttattg ctgctgttag atgtagtagt ttgagtatca gatgatgagc
    56221 tttcctgttt gttattgtca tttgcctcgt ttgatgagga aagcgagaaa cctacggtag
    56281 taaaaataat cccgataata aggagaatca taggtgtttt cttatttgtt tttttgataa
    56341 atcttacaat taacatgatt aaacctataa taaataaaac
//
LOCUS       DAZKOU010000007        53719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000007 DAZKOU010000000
VERSION     DAZKOU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.007"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..840
                     /locus_tag="ACG827_000374"
     CDS             160..840
                     /locus_tag="ACG827_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0482545.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="ACG827_000375"
     CDS             930..1964
                     /locus_tag="ACG827_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0482546.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="ACG827_000376"
     CDS             1978..2313
                     /locus_tag="ACG827_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482547.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="ACG827_000377"
     CDS             2297..4777
                     /locus_tag="ACG827_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482548.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="ACG827_000378"
     CDS             4833..6392
                     /locus_tag="ACG827_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0482549.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="ACG827_000379"
     CDS             6389..7753
                     /locus_tag="ACG827_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0482550.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="ACG827_000380"
     CDS             7924..8994
                     /locus_tag="ACG827_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0482551.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="ACG827_000381"
     CDS             9009..9677
                     /locus_tag="ACG827_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482552.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="ACG827_000382"
     CDS             9697..10275
                     /locus_tag="ACG827_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0482553.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="ACG827_000383"
     CDS             10676..11587
                     /locus_tag="ACG827_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0482554.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /gene="rnhC"
                     /locus_tag="ACG827_000384"
     CDS             complement(11965..12882)
                     /gene="rnhC"
                     /locus_tag="ACG827_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0482555.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="ACG827_000385"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="ACG827_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0482556.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="ACG827_000386"
     CDS             13579..14127
                     /locus_tag="ACG827_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0482557.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="ACG827_000387"
     CDS             14242..16602
                     /locus_tag="ACG827_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0482558.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="ACG827_000388"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="ACG827_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0482559.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="ACG827_000389"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="ACG827_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0482560.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="ACG827_000390"
     CDS             19108..20007
                     /locus_tag="ACG827_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482561.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="ACG827_000391"
     CDS             20000..21262
                     /locus_tag="ACG827_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0482562.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="ACG827_000392"
     CDS             21423..23321
                     /locus_tag="ACG827_000392"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0482563.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="ACG827_000393"
     CDS             complement(23651..24370)
                     /locus_tag="ACG827_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0482564.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    24560..24607
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24707..25039
                     /locus_tag="ACG827_000394"
     CDS             24707..25039
                     /locus_tag="ACG827_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482565.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="ACG827_000395"
     CDS             25026..27029
                     /locus_tag="ACG827_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0482566.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="ACG827_000396"
     CDS             27048..27518
                     /locus_tag="ACG827_000396"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0482567.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="ACG827_000397"
     CDS             27539..28123
                     /locus_tag="ACG827_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0482568.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="ACG827_000398"
     CDS             28138..29124
                     /locus_tag="ACG827_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0482569.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="ACG827_000399"
     CDS             29114..29425
                     /locus_tag="ACG827_000399"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0482570.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="ACG827_000400"
     CDS             29447..31228
                     /locus_tag="ACG827_000400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0482571.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="ACG827_000401"
     CDS             31221..32597
                     /locus_tag="ACG827_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0482572.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="ACG827_000402"
     CDS             32604..33239
                     /locus_tag="ACG827_000402"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0482573.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="ACG827_000403"
     CDS             33269..34624
                     /locus_tag="ACG827_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0482574.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="ACG827_000404"
     CDS             complement(34816..36210)
                     /locus_tag="ACG827_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0482575.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="ACG827_000405"
     CDS             36302..37273
                     /locus_tag="ACG827_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0482576.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="ACG827_000406"
     CDS             37270..38052
                     /locus_tag="ACG827_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0482577.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="ACG827_000407"
     CDS             complement(38221..38592)
                     /locus_tag="ACG827_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0482578.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="ACG827_000408"
     CDS             38726..39169
                     /locus_tag="ACG827_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0482579.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="ACG827_000409"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="ACG827_000409"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0482580.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="ACG827_000410"
     CDS             complement(40040..41407)
                     /locus_tag="ACG827_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0482581.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="ACG827_000411"
     CDS             complement(41391..42221)
                     /locus_tag="ACG827_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0482582.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="ACG827_000412"
     CDS             42384..42821
                     /locus_tag="ACG827_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0482583.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /gene="rpoE"
                     /locus_tag="ACG827_000413"
     CDS             42897..43517
                     /gene="rpoE"
                     /locus_tag="ACG827_000413"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0482584.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43706..44212
                     /locus_tag="ACG827_000414"
     CDS             43706..44212
                     /locus_tag="ACG827_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0482585.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44229..45128
                     /locus_tag="ACG827_000415"
     CDS             44229..45128
                     /locus_tag="ACG827_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0482586.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45215..46099
                     /locus_tag="ACG827_000416"
     CDS             45215..46099
                     /locus_tag="ACG827_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0482587.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46291..46362)
                     /locus_tag="ACG827_000417"
     tRNA            complement(46291..46362)
                     /locus_tag="ACG827_000417"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46327..46329),aa:Arg,seq:ccg)
     gene            46422..47756
                     /gene="rpoN"
                     /locus_tag="ACG827_000418"
     CDS             46422..47756
                     /gene="rpoN"
                     /locus_tag="ACG827_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0482588.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47905..48996
                     /locus_tag="ACG827_000419"
     CDS             47905..48996
                     /locus_tag="ACG827_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0482589.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            49037..50038
                     /gene="gap"
                     /locus_tag="ACG827_000420"
     CDS             49037..50038
                     /gene="gap"
                     /locus_tag="ACG827_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0482590.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50148..51338
                     /gene="pgk"
                     /locus_tag="ACG827_000421"
     CDS             50148..51338
                     /gene="pgk"
                     /locus_tag="ACG827_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0482591.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51442..52197
                     /gene="tpiA"
                     /locus_tag="ACG827_000422"
     CDS             51442..52197
                     /gene="tpiA"
                     /locus_tag="ACG827_000422"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0482592.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52313..53611
                     /gene="eno"
                     /locus_tag="ACG827_000423"
     CDS             52313..53611
                     /gene="eno"
                     /locus_tag="ACG827_000423"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0482593.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        17827 a         9355 c        11351 g        15186 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttagttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgacttgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc
    43621 ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat
    43681 taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat
    43741 cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg
    43801 tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt
    43861 tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt
    43921 ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt
    43981 ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga
    44041 gctccatatg attttagata aaatagacag tccggaaaaa gagattctga tcctctactt
    44101 ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt
    44161 taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga
    44221 agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac
    44281 aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat
    44341 catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta
    44401 tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag
    44461 atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat
    44521 ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa
    44581 caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg
    44641 aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa
    44701 tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata
    44761 gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc
    44821 cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga
    44881 tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag
    44941 aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag
    45001 agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac
    45061 cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg
    45121 atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa
    45181 taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag
    45241 tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac
    45301 cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga
    45361 acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga
    45421 atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag
    45481 ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac
    45541 aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt
    45601 aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga
    45661 gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac
    45721 ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt
    45781 tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat
    45841 catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca
    45901 gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga
    45961 aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac
    46021 gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga
    46081 tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt
    46141 agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa
    46201 aaaactgtcc accaattttc tttggcggac agttttcact taaaaataaa gaagcaacta
    46261 ttcgtaaatt atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc
    46321 aagaaccgga atcttacgtg atatccacta cactaaggac gcaagcactg ggataattgt
    46381 aactaaacag ataaaaaatt acaaggggat tggacaaaaa aatgaaattt gagcaaaatt
    46441 tttcacaaaa acagcagcag acacagaaac tagccatgac gcaacagttg caacagtcca
    46501 tccaagtttt acaatataac agtgaggagc tgttggcatt cgttgagaat caagcaatgg
    46561 aaaatccatt ggtggaagtt gttgagccgg aatggcagcc agattacata aaagcttctt
    46621 catcttctta tgaaggagaa gagacaaatt acctaaatca gattcctgat acaaaagggt
    46681 ctttatttga ttcattgatc gaacaagtcc atttgaatta ccgagatact tttttgagga
    46741 aaatcgttct ttatcttgta gaatacatcg atttgaacgg atttttgacg attagtttag
    46801 aagaagccgg aaaagagatt ggagctacgc ctatccaaat gctcgatgcg ttgactctga
    46861 ttcagcaatt agaaccagct ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc
    46921 aaacagaacg agatgattat gcgccggaat tagcttatat cgtgttggaa gaatgttttg
    46981 aggaactggt ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt gatctacatg
    47041 ctgttcagca gatattcgac tatttacaaa cgttgagtcc aagccctggc cggattttcg
    47101 accgtagcag cgaattgtac attattcctg atgtccgggt attagtggac gacgataaaa
    47161 atgtacaagt tatctcaaac cgaaaaaatc agccaaatat acgctttcaa gagagttact
    47221 tcaaacaaat gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa gaacggaaac
    47281 aggaattcga atggttgaag aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg
    47341 ctcaagtcat tgtttcccgg caaaaagctt tctttatcga caaggaacgt ccaatcaagc
    47401 ctcttacgtt aaaggaagtt gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg
    47461 cagtcaacgg gaaatatttg gaaacagact tcggtgtatt tgaattaaaa aaatttttca
    47521 ctacacgaat tcctacgaat agtacagaac agacagaaga cctttcagca gatacagcga
    47581 agaaaaagct gcaggaatta gtcgatcaag aagacaaaaa caagccgttg tctgaccaga
    47641 aattggttga attgctgaaa aaagatgaga tagcgatctc aagacgaaca gtagcaaaat
    47701 atcgtgattt attaggaatc ccgagttcat caaaaagaaa acgttatgat aactaatcag
    47761 tcaaaagact agaaaaagta tttcactttt tctagtcttt ttagttgaaa aacgagagca
    47821 aaactgttag acttacagtc gtgaggttga gatttccctt tattgatgtg aaattcgaat
    47881 agttaatttc tttttttatc gtatatgggt cgttttaagt ctacattgga cgtaaagtgt
    47941 ccaactaagg agatcgctat gctggatgaa ataaaaatga ttgaatctgt tgcaccagat
    48001 attatcgaag tgttgcaaga acgctataaa attttacgaa atatctattg gatgcagccg
    48061 attggacgtc gaagtttatc ggaaagtatg gggatcacag aacgagtatt gcgaacagaa
    48121 acagatcttt tgaaaaatct gaatttgatc gacacctcaa aaagcggtat gacgctgaca
    48181 actaagggcg aagaagttta tcaaagcctt gaaaacttca tggatcagct tttaggtacg
    48241 catcaaaccg aacaacagtt ggcagaatat tttggtaacc aacgctgtat cgttgtttca
    48301 ggaaacagcg aagagcagac caaagtggct gatgcattcg gcgaagcgct gagcgaagcc
    48361 ctagaccgac tacttccaga aggcgagaat atcatcgctg tgatgggagg aactactatg
    48421 gctgtggtag ccgaacaatt gtctaatctg gaaaataaga aacggcataa cctgtttgtc
    48481 cctgcaagag gcgggattgg tgaagcgata actgtacagg caaattctgt cagtgcaaga
    48541 atggctgcta aagcaaacgg taaccaccgt gcactttatg ttccggaaca attaagtctg
    48601 gcaacttaca attctttact caatgagcca tctatccaag aagtcttaaa cttgattagt
    48661 gaagcgaatt gtgttattca tagtattggg cgtgcattac acatggcagc acgtcgtaag
    48721 atgactgaaa aagaattagt catgctgaaa caagcaaatg ctgttgcgga atcttttggc
    48781 tactttttca atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg catagaattg
    48841 caagatttgg aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa
    48901 gcaattcgag cttatatgaa aaatgcacca aaacaaacgt ggctcatcac tgatgaagct
    48961 gctgcaaatg agattttaaa aggggtaacc ctttaaaata aatatttttt tgattttcat
    49021 aaggaggaaa tcttaaatga cagttaaagt aggtattaat ggttttggac gtattggacg
    49081 tctagcattc cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt
    49141 gacagatgct aaaatgttgg cacacttgtt aaaatacgac acaactcaag gacgtttcaa
    49201 cggaacagtt gaagttcatg aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt
    49261 agctaaccgc aacccagaag aattaccatg gggcgaacta ggcgtagata tcgttctaga
    49321 atgtactggt ttcttcactt ctaaatcagc agctgaaaaa catttaacag ctggtgctaa
    49381 acgcgttgtt atctctgctc ctggcggaaa cgatgttcca acaatcgttt ataacacaaa
    49441 ccacgaaaca ttaactggta aagaaacagt tatctcaggt gcttcatgta ctactaactg
    49501 tttagctcct atggctaaaa cattgaacga caaatttggt gttgttgaag gattaatgac
    49561 aactatccac gcttacacag gtgaccaaat gactctagac ggacctcatc ctaaaggtga
    49621 cttccgccgt gcacgcgctg ctgctgcaaa catcgttcct aactcaacag gtgctgctaa
    49681 agctatcggt ttagtaattc ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt
    49741 tcctgtacca acaggttcat taacagaatt agtaacagtt cttgaaaaag aagtaactgt
    49801 tgacgaaatc aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat acaacacaga
    49861 cgaaatcgtt tcttcagata tcgttggtat gacttacggt tcattatttg atgctacaca
    49921 aactaaagta atgacagttg gcgacaaaca attagttaaa actgttgctt ggtacgacaa
    49981 cgaaatgtca tacactgcac aattagtacg tactttagaa tacttcgcta acttataaga
    50041 tagacaaacg tacaagtgta aattatagac atgtacattt aaaagcgggg aagcaatcgc
    50101 gcttcttccg ctttttttga taataagttt attcaggagg aaataaaatg gctaagaaaa
    50161 cagtgaaaga tatcgattta aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc
    50221 ctttgaaaga cggcgtgatc actgacgaca ctcgtatcaa agcagcattg ccaacaatca
    50281 actacgttct agaacaaggc ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa
    50341 cagaagaaga taaagaagga aaatctttag ctccagttgc aaaacgtcta ggcgaattat
    50401 taggtaaaga agtaacattc gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc
    50461 gcaacatgaa tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa gatattgacg
    50521 gtaaaaaaga aagcaaaaat gatacagaac taggtaaata ctgggcttca ttaggcgatg
    50581 tatttgttaa cgatgctttc ggtacagctc accgtgcaca tgcttctaac gtaggtatcg
    50641 cttcaactgg tatcccaaca gttgctggat tcttgatgga aaaagaaatc aagttcatcg
    50701 gtgaagcagt agaagaacca aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt
    50761 ctgataaaat cggcgtaatc gaaaacttga ttcctaaagc agataaaatc ttgatcggtg
    50821 gcggaatgac ttacacattc tacgaagcaa aaggaatcaa gatcggtaac tctctagtag
    50881 aagctgataa agtggaacta gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct
    50941 tgccaatcga caatgtctgt gcaccagaat tttcaaatga tgttgaaaca caagtaatcg
    51001 aaggagacat tcctgatggt ttgatggctt tagacatcgg cccagcatca gtaaaattat
    51061 ttgctgatac attacaaggt gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg
    51121 aaatgagtaa ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct aacttagaag
    51181 gcgcaacaac aatcatcggt ggcggagact cagctgcagc agcagaacaa ttaggatttg
    51241 ctgacaaatt cacacacatc tcaacaggtg gtggcgcaag cttggaattg cttgaaggta
    51301 aagaattacc aggactagca gcaatcaacg ataaataaac gaccaagttt gctcaattct
    51361 gacgagtaat cgatcaaaag gtgaagggag ttttcatgac tccctagcca tttttcaata
    51421 aattaaggag tgtgttcctc aatgcgtaaa cctattatcg caggtaactg gaaaatgaac
    51481 aaaactgcat cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat cccttcaaat
    51541 gacaaagttg attcagtcat cggatctcct gcattattct tacaagaatt aatgacatca
    51601 gctgaaggta cagaactaaa aatctcagca caaaactgct actgggaaaa ctcaggtgcg
    51661 ttcactggtg aaacttcacc agcagctctt gctgatctag gtgttcatta tgtgatcatc
    51721 ggacattcag aacgtcgtga atatttccat gaaacagacg aagacatcaa taaaaaagca
    51781 aaagctatct ttgcaaatgg tatgacacca atcctatgct gcggcgaatc tttagaaacc
    51841 tatgaagcag gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg tttagctgat
    51901 ctttcagctg aacaagtgtc aaacatggtt attgcttatg aaccaatctg ggcaatcggt
    51961 actggtaaat cagctgacgc acaaatcgct gacgaaattt gtggagttgt acgtcaaaca
    52021 gttgaaaaac tttatggaaa agaagtatca gaagcagttc gtatccaata cggcggttct
    52081 gtaaaaccag aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc
    52141 ggtggcgcaa gcttgcaagc agattcattt ttgtcattgt tagacgctgt aaaataattg
    52201 ttgaaaattt gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt aactaaaatc
    52261 gtatatgagg gaaatccctt aggaaaaaac aaactcaaag gagagacaaa acatgtcaat
    52321 tattactgat gtttacgctc gcgagatctt agactcacgt ggtaacccaa caatcgaagt
    52381 agaagtatac acagaaagcg gagcttttgg ccgcggaatg gttccttcag gtgcttcaac
    52441 tggtgaatac gaagcagttg aattacgtga cggagacaaa gctcgttatg gcggtaaagg
    52501 tgtaactaaa gctgtcgaca acgtaaacaa catcattgct gaagcaatca ttggctacga
    52561 tgttcgtgac caaatggcta tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa
    52621 aggtaaatta ggtgcgaacg ctattttagg tgtttctatc gctgttgcac gtgcagctgc
    52681 tgactaccta gaagtacctt tataccacta cctaggcgga ttcaacacaa aagtattgcc
    52741 aacaccaatg atgaacatca tcaacggcgg atcacatgct gataactcaa tcgacttcca
    52801 agaattcatg atcatgcctg ttggcgctcc aacattcaaa gaagctctac gtatgggtgc
    52861 tgaagtattc cacgcattag cttcaatctt gaaagctcgc ggattagcaa cttctgttgg
    52921 tgacgaaggt ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat
    52981 cgaagcaatc gaaaaagctg gctatgtacc tggtaaagac gttgttcttg ctatggatgc
    53041 tgcttcttca gaattctacg acaaagaaaa aggtgtttac gtactagctg actcaggcga
    53101 aggcgaaaaa acaactgacg aaatgatcaa attctacgaa gaattagttt ctaaatatcc
    53161 aatcatctca atcgaagatg gattagacga aaacgactgg gacggattca agaaattaac
    53221 tgacgtatta ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa caaacactca
    53281 aaaattatct gaaggtatcg aaaaaggaat tgctaactca atccttatca aagtgaacca
    53341 aatcggtaca ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag ctggctacac
    53401 agcagttgta tctcaccgtt ctggtgaaac agaagattca acaatctctg atatcgctgt
    53461 tgcaacaaat gctggccaaa tcaaaactgg ttctctatca cgtactgacc gtattgctaa
    53521 atacaaccaa ttactacgta tcgaagacca acttggtgaa gttgcagaat acaaaggttt
    53581 gaaatctttc tacaacttaa aaaacaaata attcttttct aatgaagata catgatcgac
    53641 tattgaaaga tagtcgagta tgatcactta gttaagtaat cgaactctct acttgctgaa
    53701 atatcagcag gtagagagt
//
LOCUS       DAZKOU010000008        53561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000008 DAZKOU010000000
VERSION     DAZKOU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.008"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG827_000424"
     CDS             <1..878
                     /locus_tag="ACG827_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0482594.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
     gene            complement(1119..2735)
                     /locus_tag="ACG827_000425"
     CDS             complement(1119..2735)
                     /locus_tag="ACG827_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0482595.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            2853..3071
                     /locus_tag="ACG827_000426"
     CDS             2853..3071
                     /locus_tag="ACG827_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0482596.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3266..3727
                     /locus_tag="ACG827_000427"
     CDS             3266..3727
                     /locus_tag="ACG827_000427"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0482597.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3920..4159)
                     /locus_tag="ACG827_000428"
     CDS             complement(3920..4159)
                     /locus_tag="ACG827_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0482598.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4183..5706)
                     /locus_tag="ACG827_000429"
     CDS             complement(4183..5706)
                     /locus_tag="ACG827_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0482599.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(5724..5846)
                     /locus_tag="ACG827_000430"
     CDS             complement(5724..5846)
                     /locus_tag="ACG827_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0482600.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(5876..6859)
                     /locus_tag="ACG827_000431"
     CDS             complement(5876..6859)
                     /locus_tag="ACG827_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0482601.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="ACG827_000432"
     CDS             complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="ACG827_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0482602.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(7053..7430)
                     /locus_tag="ACG827_000433"
     CDS             complement(7053..7430)
                     /locus_tag="ACG827_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482603.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="ACG827_000434"
     CDS             complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="ACG827_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0482604.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="ACG827_000435"
     CDS             complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="ACG827_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0482605.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(8448..10214)
                     /locus_tag="ACG827_000436"
     CDS             complement(8448..10214)
                     /locus_tag="ACG827_000436"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482606.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(10174..11928)
                     /locus_tag="ACG827_000437"
     CDS             complement(10174..11928)
                     /locus_tag="ACG827_000437"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482607.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(12038..12607)
                     /locus_tag="ACG827_000438"
     CDS             complement(12038..12607)
                     /locus_tag="ACG827_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0482608.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(12625..14040)
                     /locus_tag="ACG827_000439"
     CDS             complement(12625..14040)
                     /locus_tag="ACG827_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482609.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(14051..14719)
                     /locus_tag="ACG827_000440"
     CDS             complement(14051..14719)
                     /locus_tag="ACG827_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0482610.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(14849..15433)
                     /locus_tag="ACG827_000441"
     CDS             complement(14849..15433)
                     /locus_tag="ACG827_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0482611.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(15764..15997)
                     /locus_tag="ACG827_000442"
     CDS             complement(15764..15997)
                     /locus_tag="ACG827_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0482612.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(16012..16962)
                     /locus_tag="ACG827_000443"
     CDS             complement(16012..16962)
                     /locus_tag="ACG827_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0482613.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16959..17801)
                     /locus_tag="ACG827_000444"
     CDS             complement(16959..17801)
                     /locus_tag="ACG827_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0482614.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(17803..18522)
                     /locus_tag="ACG827_000445"
     CDS             complement(17803..18522)
                     /locus_tag="ACG827_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482615.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            19070..19360
                     /locus_tag="ACG827_000446"
     CDS             19070..19360
                     /locus_tag="ACG827_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0482616.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
     gene            19432..20658
                     /locus_tag="ACG827_000447"
     CDS             19432..20658
                     /locus_tag="ACG827_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0482617.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20746..21138)
                     /gene="rpsI"
                     /locus_tag="ACG827_000448"
     CDS             complement(20746..21138)
                     /gene="rpsI"
                     /locus_tag="ACG827_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0482618.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(21154..21582)
                     /gene="rplM"
                     /locus_tag="ACG827_000449"
     CDS             complement(21154..21582)
                     /gene="rplM"
                     /locus_tag="ACG827_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0482619.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(22116..22583)
                     /locus_tag="ACG827_000450"
     CDS             complement(22116..22583)
                     /locus_tag="ACG827_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0482620.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(22703..24241)
                     /locus_tag="ACG827_000451"
     CDS             complement(22703..24241)
                     /locus_tag="ACG827_000451"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0482621.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            24321..25007
                     /locus_tag="ACG827_000452"
     CDS             24321..25007
                     /locus_tag="ACG827_000452"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0482622.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(25208..25735)
                     /gene="msrA"
                     /locus_tag="ACG827_000453"
     CDS             complement(25208..25735)
                     /gene="msrA"
                     /locus_tag="ACG827_000453"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0482623.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(25819..26496)
                     /gene="pgmB"
                     /locus_tag="ACG827_000454"
     CDS             complement(25819..26496)
                     /gene="pgmB"
                     /locus_tag="ACG827_000454"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0482624.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(26493..27623)
                     /locus_tag="ACG827_000455"
     CDS             complement(26493..27623)
                     /locus_tag="ACG827_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0482625.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(27623..29806)
                     /locus_tag="ACG827_000456"
     CDS             complement(27623..29806)
                     /locus_tag="ACG827_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0482626.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(29803..30828)
                     /locus_tag="ACG827_000457"
     CDS             complement(29803..30828)
                     /locus_tag="ACG827_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0482627.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(30873..31700)
                     /locus_tag="ACG827_000458"
     CDS             complement(30873..31700)
                     /locus_tag="ACG827_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0482628.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(31697..32764)
                     /locus_tag="ACG827_000459"
     CDS             complement(31697..32764)
                     /locus_tag="ACG827_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0482629.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(32790..33626)
                     /locus_tag="ACG827_000460"
     CDS             complement(32790..33626)
                     /locus_tag="ACG827_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0482630.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(33623..34492)
                     /locus_tag="ACG827_000461"
     CDS             complement(33623..34492)
                     /locus_tag="ACG827_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0482631.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(34562..35845)
                     /locus_tag="ACG827_000462"
     CDS             complement(34562..35845)
                     /locus_tag="ACG827_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0482632.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(35859..37544)
                     /locus_tag="ACG827_000463"
     CDS             complement(35859..37544)
                     /locus_tag="ACG827_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0482633.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            37819..38844
                     /locus_tag="ACG827_000464"
     CDS             37819..38844
                     /locus_tag="ACG827_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0482634.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(39439..43092)
                     /gene="rpoC"
                     /locus_tag="ACG827_000465"
     CDS             complement(39439..43092)
                     /gene="rpoC"
                     /locus_tag="ACG827_000465"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0482635.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(43171..46788)
                     /gene="rpoB"
                     /locus_tag="ACG827_000466"
     CDS             complement(43171..46788)
                     /gene="rpoB"
                     /locus_tag="ACG827_000466"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0482636.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            47516..48484
                     /locus_tag="ACG827_000467"
     CDS             47516..48484
                     /locus_tag="ACG827_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0482637.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            48502..49407
                     /locus_tag="ACG827_000468"
     CDS             48502..49407
                     /locus_tag="ACG827_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0482638.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            49404..50210
                     /locus_tag="ACG827_000469"
     CDS             49404..50210
                     /locus_tag="ACG827_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482639.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            50367..51335
                     /locus_tag="ACG827_000470"
     CDS             50367..51335
                     /locus_tag="ACG827_000470"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0482640.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(51652..52176)
                     /locus_tag="ACG827_000471"
     CDS             complement(51652..52176)
                     /locus_tag="ACG827_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0482641.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            52463..>53561
                     /gene="scm"
                     /locus_tag="ACG827_000472"
     CDS             52463..>53561
                     /gene="scm"
                     /locus_tag="ACG827_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0482642.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTST"
BASE COUNT        16025 a        10928 c         9138 g        17470 t
ORIGIN      
        1 tttctttgag ccagctgtta ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa
       61 atgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctataaaag
      181 tccgtgaaca agtgcgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacattg
      301 gtcgggcttt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
      361 ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gattccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgttat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatac caaacagaaa
      661 tcaaacgagc gttgcgcgtt ccctattcca acggccctat agaatgtttg aataaccata
      721 tcaaagtact aaaacgaatt gcctatggct atagaaactt tcaaaactac cgagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa tactacccaa ctgactaaag
      841 ccagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcttcaccaa cactcattga cgaagagcca aaataagtaa tctaaccgac
      961 tgttaactaa accattgcag gtcccgttaa agatacttgt gcctgttgtc accgcttctt
     1021 tttcttgtcc acaagcgctt aacacactta ataaagcccc tgctattatt aataataata
     1081 tctttttctt attaaaaacc tcctcatcta tttccagatt agtaaatgta gtaatctgtg
     1141 ctttcttttt tctcaatact aataactaac ttatggggta aacaaatact cgtttgaccg
     1201 ggttttgaga tccatccagt tctaaccgcg atttgatcag gactattatc ttctttaact
     1261 cgaattcttc catctttaat ctcaacaata ttatattgtc ccttcgcagg atgataattc
     1321 acgagtttat gattgatctt atccaaatcg aagcgatcca tttccttgcc atcaattcga
     1381 acgatggcat atttgatagc atcttcagtt gtttcattct ccttttctat agagaataca
     1441 aataagggaa taaaagaggt aataatcaaa atgccaataa ttatgacatc gcctatcttg
     1501 acaccagctt ttcttaaaaa tttcattaac gatatctggc taaacatatt agactttttc
     1561 catggaaatg gtgaaatttt tcggaaatta tctgcattaa gagaatcagt taaacaaccc
     1621 tcaagttgtt ccttaataca ttgttgatcc atcttcatac caataaaaac tagtttatta
     1681 atgcgttcac caaacaacgg atgccaggct tccttcaatt cgggattttt ttgtataatc
     1741 atttgacact caattttagg taaactagct acccaataat ttaatgattc aatactttta
     1801 tagctacccg atgtttcaaa gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac
     1861 ccttttgctc ttgtaatatt gccaggaaaa tttttatccg taagatgggc aaatttttgt
     1921 gcgtcaagga gtaaccgatt tcgatagata aaacttgaga taccatattc ttctgtttca
     1981 ggaatatgat tttgatagcc aagctctaac tctttgaacc agcccactga ataggaagtc
     2041 tcatctatat taaaaagatt tgtatttaga attttcgata agtctacttc acctttaatt
     2101 gtcctaatta ttttggcatt ggactgtaac ttagcaatta gaccgttaat tttgtaaatc
     2161 gtcttggttg acactaagtc actattattt aaaagtaata tgttacaaca ctcgaattga
     2221 tcaattaaca agccacgaat atcctgttct tcttcaacta cattaccttc agcttttctt
     2281 tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac gttttacatc cactactata
     2341 ataatggcac caatctctaa tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat
     2401 ataattgctt gaataattgg aattggtttg ctgattcctg aagcttcaat aacaatttga
     2461 ttcaagtcag aaaattgcac taattgatct agttcaacaa tcaaatcttc gcgtaatgta
     2521 caacaaatgc atccattcgg catcgaaaca agtttttctc cagttctttt aaaacctttc
     2581 ttgtcgacta ataccccatc aacatttact tcccctatgt catttacaat gacagcaatt
     2641 tcctttccgt gatcaccgta cagaatatga tttagaagta tcgtttttcc tgaaccaaga
     2701 tagccagtta agattgtaat aggtattttt ttcataggga acctctttct ataccttgaa
     2761 tttacacttt aagtgcaaca cataaaaatg agtcataatc gatatccaac tagtataagt
     2821 ttgtcgagaa cactcctaga aggaaaataa ttatgagcca gtatcgtcat cttactatac
     2881 tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt ttctattagt ataattggca
     2941 acgacattca gcatagcccg tttactattt tacatgaact gagaagaaac aagaaaaaaa
     3001 ctattcacct tccaaagcgg aaaaaaatta tgcttatcga agaaaaaaat gcggacgtta
     3061 tcacattcta aatgatccgt gagtttggtc aattgttcgt catttatttg tcgattttca
     3121 gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa catatggact tttcaattag
     3181 ttttatcacg atctaacgtg caatttgcaa tgatcttttc gatacagaac ccctctcaca
     3241 cggcaataaa ggcctgatcc gttcattgca ccatcgagga aaaacaagac atacaaaaaa
     3301 tcatgtcgag tctcgtggaa agatttgcct tactcattcg atccatggcc gtccaaaatc
     3361 cgcagatgat agaagtgatt ttggtcattg gaaagccgat attgtagctg gcaaaactgg
     3421 tggtacttgt ttagtgactt tgacagacag aaaatcatgc ttccttttag caggaaagaa
     3481 ggtccccaga aagaaagccg cttatgtgag cgagaaaatg attgaacttt taagcagtat
     3541 tccaaatgag gcggtcaaaa cgatcactcc agatagagga aaagaattct ccaatcattt
     3601 cgcagttaca gaagcattga ataatatgcc cttttatttt ccagatcctc atgcccttgg
     3661 caacaatgaa caaattaaaa tacgaataga ctcattcgag aatacttgcc aaaatcgaaa
     3721 gagatagcta cggtagataa ctcaacgata aatcagatga ttgaaaaact gaataacaga
     3781 cctagaaagt gtttagattg gagaacacct tatgaagtct tctttgataa agtgttgcgc
     3841 ttgatttgac aattcaagac ataataaaaa gcagatcaaa tccaaataag ggaaaaatga
     3901 tctactctgg tattatcaat taattggcca agctattatg ccgttttgct aaacgtctac
     3961 ttcttccagt attgaccttg ataaaacaac aatcaggatc ccgttttaat actctatgaa
     4021 ctgtgtcaat actaacatgt aaagttgcag caatatcagc taatgtactc ccatttaatc
     4081 tcatttgctt tgcaaatcga taacgccgat aagcatcaat attcttggaa tccatagaca
     4141 ataatacttt tcttaccatg tatttcctcc aaaaattgaa ttttaatgag ccatcatttc
     4201 ttgagcaatt tcatgtttag acaatgaagc tggatagaaa gtaggccaat tatgcaattc
     4261 attatagagt ttttcttgac tttctccacc aaagaaaata tggaaatgct ctgccttact
     4321 tggtgcaatt ccatgatcac taaattgaat atatttaaaa cgaccagcac ctgcatcatc
     4381 agtttcaaaa ttaaaacgaa caccacggtt tcctttttca taattcaaaa ttttgtatcc
     4441 aacatatttg tatgtgtatt gatgatgttc accgttgact acaaaatcaa tggtgttgtc
     4501 tttaatgttg atagtatcaa tatctgtctt atagccagta gtataataat ctttgtactc
     4561 ggcagctgtc atgtctttat tgatttttgc tttgtaatcg aaaacttcat ccaaaattcc
     4621 atcttttaac aacggataaa cagattgcca ttcgcctgta taatcactca attcgcgatc
     4681 cttcacatcg gcatctttaa agtacccatt agcaactgtc ttttcatcct tcgcttcttt
     4741 ttctggaact acctcttcac cagcaaccat agttgttttt tctaacgctt ttagattgtc
     4801 ttccattact gaaacataat cttcaccatt atccatttgt tcttttgtaa gactttcaag
     4861 aggattcagt acttccaatt ttatcccagc ttcgttggcc aaagtttttg caattttatc
     4921 attagcattc ttttcaaagt aaatataatt gatcttattt tttttaacat actcttttaa
     4981 ttcagctaat cgaccagaag atggctcttc ttcaggactt agtccagcaa taggaacttg
     5041 tttcaatcca taatccaaag ctaaatatcc aaaggctgcg tgttgcgtca caaaactttt
     5101 ttgtttcgct tctttaagac tagtggtata gtcagcatcc aaccgtttca gtttagtcaa
     5161 atacttttca gcatttgtct caaatacttt tgcttttttc ggatacaatt taactaactg
     5221 atccttgata tttgaaactt ccttaattgc cattttgggt gaaacccacg tatgaggatc
     5281 tagttcatga tggtgatctt cttctccgtg atcatgatct tcttcttcac tacctggcaa
     5341 taaaatcatg tcttttgttc cttctactac attaggccca gctttcttcc aactttcttt
     5401 agcttttggt acccatgatt ccatgttttc gttatgatat acaaaaacat cagcatcatg
     5461 agcttttgcc atatccttag ctgagggctc atagtcatga ggttctgatc ccgctggaac
     5521 cattaaatcc acgttcgctt catcgccgac gatgtttttg gtgaaatcat acattggata
     5581 gaatgttgtt acaatttcta attttccatc tttatcattc gatacttttg catcattagt
     5641 gctggagcaa gctcccaata atcctagagc tgctatccca ataagtccta cactgatttt
     5701 cttcattctt tttccttcct taattatgct aatttcttca ttttatttcg tttatgttct
     5761 ttgataattt ttaatgccct ttttctagtt ttaatattcg ggcttttaag tcgtcgatat
     5821 gcactagata tatccgtttt ctccattaga aattaccccc aatcactact tatcattatt
     5881 tgctttccgc cagtaattta tcaaaactct gtttgatcct ttcttcatcc aacatttttc
     5941 cgattagaat aatagtggtt tggtcttttt gtcgagtggt gtattgaaaa ttaacctgtt
     6001 cgtttacctc ttgcatggca actataaaat cctgctcttg taaagcaatc aatcctttaa
     6061 aacgatacag gctcccttgg ttggttaaaa tcagccagtc aatccattgg agtaaaaaat
     6121 ctttttttat gtcttgatct gaagttaaga gaattgattt aaactcatgt acttgatggt
     6181 gttgatgata gtatttgcca ttatagtgat tttctgatga atcactttca aaaactaagg
     6241 gatgattaaa ttttttcaaa ttataaaaat cctttttttg aatcgtttca ctatgtgtga
     6301 acattcgaat atcggataaa gggttaatac ttttgatttc tctctgtaat ttttttagtg
     6361 cagataaatt caagttttgt tttctagaca caaaaatgcg atctgccatg acaagttgct
     6421 taagggcttg tggttctctt tcaataagtt caaaactgtc actatcaata acagtcaata
     6481 aactatcgat atatactctt cctcttagtt gaggcgctgt taaaagtgtt tgaatgattg
     6541 gttggggatc tgctatacct gtagtttcaa tgatgatttg tgaaattggt tgttgatgtt
     6601 cttctattgt ttccagtaaa gaaatcaata ttgctaataa atcactgcga agactgcaac
     6661 acatacatcc attatttagt tgtaacaccc gttcctcgct atgaactaat aatttgtgat
     6721 caatcccaac ttcaccaaac tcattttcaa ttataataat ttcttccctg gggtagggtg
     6781 agacttgcaa ggcttgattg attagcgttg tttttccagc ccctaaaaac ccagaaacga
     6841 ttgtaactgg tatgcccatt ttgtcacctt tttaccactt agctatttca cttctttaaa
     6901 aattgcccgt ctgcgcaatt ttggcgaata cttttttaat tctaaccgtt cagggttatt
     6961 ccgtttattt ttgctggtga gatacagacg ttctccagtt tcgacacatt ccaaaataat
     7021 attttgacgc ataaatcttc ctccttcaat acttatcgat cttcaagata tgcttgaatt
     7081 tgttgtgcat cttcaaaagt taactcgcca gattccaatt gtgaaactaa attttctttc
     7141 atatcaacag gtatctccca ataaccatcg atacattgaa ataactcctc gtcatactct
     7201 tcaaaaattg gatagattct taaaaaatca tcaaatgata agccttggtc ctcatagtaa
     7261 tctttgacta atccattttc aattgcctgt tgggtcggta aacccgtttc agtaataatt
     7321 cctctctcta ccatcgaacg ataaaaagtg catttatcta gcgattgctg ggtcgtttca
     7381 agataatact ttaatccttc ttcaactaaa ggtgggtaaa taatttccat tttgattccc
     7441 tcctaaaagt tatatattaa actattttga agctccttta attactttct ttccattata
     7501 gtaccctttt aaagatacat gatggctacg tctataatca ccattcgctt cgtcaaatga
     7561 aatttctggt tttactacag tttgatgagt tcttcttaac cgtttcttcg ccttagatgt
     7621 ttttcttgct ggtactgcca tgatatttcc tccttttacc aacttgcttt ttttactcca
     7681 ggaatcaacc cttgatgggc aagttctcta aacttaatac gggacatacc gaactttcgc
     7741 atataccctc ttggacgacc atccgtctga tcacgaagcc tcagacgatt cggatttgaa
     7801 tctcttggca atttagcgag agcggtgcga tctcctgcgg cttttaattc ctgacgttta
     7861 tctgcatact tttcaatcat cgccatttgt ttctttgctt tagcaatttt cgactttttt
     7921 gccatatacc tttatactcc ttttattttg atttagacac ctcaaatcgt aatcgttacg
     7981 ttttgacact gatactttac caacaagagt tgctcttgtc aacaataaat aacatgaaat
     8041 aaaactcata tgaatagata actatttgaa aacgaattta tcggtacata gagataatca
     8101 aatcatttaa aagaatatgc agtttaggct ctgaaaaata agaattcact tttattagtc
     8161 caatcaaaca caaataccgt tttaagtagc ttttcctcta cttaaaacgg caatatgaaa
     8221 gaaactataa ctttttattc aatcttttat gtttaactgg tttatcgcca attttttaaa
     8281 actttgatta ttccttatat tataagaata gacattaagt gaactaattc aatcatactg
     8341 attctttcgt gtattcaaaa ttagatgctg tttaataaaa ttattctttt tgaaaaagaa
     8401 ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca tcgtgattga
     8461 gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag atttttgtat
     8521 atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt tccttgatcc
     8581 atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat aattagtact
     8641 gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt ttccgcatct
     8701 aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa agcccgtgct
     8761 atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc gaccaatgta
     8821 tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg agctgctttt
     8881 ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa aatacttgta
     8941 ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga agctggagat
     9001 acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc atacagtcta
     9061 gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa cgcagtcact
     9121 tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt atcatatgag
     9181 aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga tccctcttgg
     9241 atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata aaatatttca
     9301 agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc tagtaagtaa
     9361 ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc tactagaatg
     9421 actactgcta aactaacata tgagaaaata gaggataagg ccatgactga aaaagtactt
     9481 aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag tctgtcttga
     9541 acttttttag atagattaaa gctattaatc tcttgctgca attctatagt ttcttgaaat
     9601 tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt ttgggatttc
     9661 tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc taatcccatt
     9721 ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc cataccgccg
     9781 gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga catgatagtt
     9841 tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt tgacaaatcg
     9901 tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata taagttttca
     9961 tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac ggcaaaataa
    10021 agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat tataatgaat
    10081 gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt agttaaagct
    10141 tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat tgtcaagcct
    10201 ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat gtttagccat
    10261 taattcatta tgatttccac gttctactat tttcccttct gacaagacaa gaatttcatc
    10321 tacagattta atgctggata atcgatgagc tatcataata actgtttttt ctttcatcag
    10381 atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg cactggcttc
    10441 atccataata acaatctttg catcttttaa aattgctctg gcaatagcta tacgttgttt
    10501 ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc tttcgggaaa
    10561 tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt tacttgctgg
    10621 cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata gtttcgtatc
    10681 ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat ttaaaggctt
    10741 accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta gtttagctat
    10801 cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt gttctaaaac
    10861 taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat tttcaaatgc
    10921 aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt gcttcatttc
    10981 taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt gcatacttac
    11041 atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca acatcaacaa
    11101 gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta taggaactat
    11161 caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact
    11221 caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca aggctttaaa
    11281 tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat attctaccgt
    11341 ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg tattctctgt
    11401 cattttcttt aacaagaata caccaattac taacatggcg attagaacca atccaatttt
    11461 aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg cttgagcact
    11521 atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat caattgtctt
    11581 tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct
    11641 aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag aaaataagcc
    11701 agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa ccgcataact
    11761 cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt aaatactgta
    11821 atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta accatccgta
    11881 gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt tttcctcccc
    11941 cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta atagcgtaaa
    12001 ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga
    12061 cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac actgaaatga
    12121 ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca gattgtcctt
    12181 tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc cactgtccgg
    12241 cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct cgatcatatc
    12301 caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg ataaaccaag
    12361 gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg taaatagtaa
    12421 aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca gataataatc
    12481 caaaaatccc gggggatatg ctgtgaccaa acgccccacc aatactacta accattattg
    12541 aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaata taatcttttg
    12601 ttttcatata tattctcctc aattctattt cgttatgtca ttaaaaatct gttctatcaa
    12661 agttgaatct tctactctaa gttttatatc tttttttatt ccaccatttt ttaaataaat
    12721 aattctattg catgttttat aaagaaattc cggatcatga gtagctataa ctactgcacc
    12781 cttttctttt aagtctttaa taaggtcagc aatcctttcc atattaatat aatccaaccc
    12841 acttgtaggt tcatcaaata gaaataaatt agtttttgac aatgaagcta tagaaaccaa
    12901 taatctttgt ttttctccac cggataaatc aaacggtaat tgatctttca ttttataaag
    12961 ttccattttt tttaaaattt tttcttgctg atctttacta actatttcat tccccagggc
    13021 taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc atttcttgca ttactaaaaa
    13081 cggcgatgac aaattttttt tattttcgat tatctttcct ttggtagggc gaataatctt
    13141 catgagtatt cttagtaagg ttgttttccc cacaccgttt ttccctacca aaccaataac
    13201 atctccaact ttgacatcaa aatttatgtc ttttagaata ttttcataag taatttcttc
    13261 aacttttaat acatctgcac ttaccttttc gatataggga acttcaatcg aactattaaa
    13321 aatgtcaaat ttccttaatg cgttattgtt ttcagttttt aacaactgaa tagttgtagt
    13381 ttttacagtt ctatttttaa caaatagaac ttgatcaatt aattctttca aatagtaaaa
    13441 acgatgttca gctataacaa tagtaaatcc tttatttttt aaatcacaaa gtaagtcagc
    13501 aagtttctta gtcgctttga agtccaaatt tgaagacggt tcatccaata taattaattt
    13561 agagtttagc gataaactac atccaataga aattttctgc cgttcaccac ttgatagcga
    13621 aaaaatgttt ttatctaata tatttgaaag atctaataat tcagctaatt cattgattct
    13681 tttatccata tgctcttttg taaatccaaa gttttccatc gaaaatacta actcactcgt
    13741 tgtatttgtt gtaaagaatt gtcctctagg atcttgactt acgtagccaa tattttttga
    13801 tatactttct tgtgttaagg attgtagcga gttattttca aataaaattt ccccaaccaa
    13861 ttttccgtca taaagtcttg gaataagtcc attcattaac tttaatagtg tggatttccc
    13921 actcccagaa gaaccagtaa tcaatgtaag actgcctttt tttattgaaa aattaaggtc
    13981 aacaagcgct tgttccttaa catccttata tgaaaaagaa acattgttaa attcaaacaa
    14041 ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt aacggaaaac aggatgatat
    14101 agtctaataa gctcatgttt gttttatagt aagaacttct tttcacagga gattctatcc
    14161 cctttgttat tgcattagcg gtcatttctt cagcaatcat agttgcacgt ataagaacgg
    14221 gaactaacag aaattccaaa tattgcagag gctgaaaaaa agacaagcga atgtttctca
    14281 tttttaaaga ttctttaatc attcttattt cagaacgata agtcggaaag aatctgatca
    14341 ctactgtaat acttaacatt atttttttcg gtaaatgtat tttttctaaa gaggataata
    14401 attcattgct agtatagaca ctaaccaaca accatgagat cattacgacc ggcaacattc
    14461 gaaatacaat atataaggat gtcgcaaaaa atatagtaaa aaactctatc ttgatagcat
    14521 caataatttt cattactaaa aacagaactg aataaatcaa gatgaaataa atacatgtct
    14581 ttattttacg aaacatcaat actaaaactg tagcaataat gaaacacccc acttgaaata
    14641 tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat atttaaagta atcaaggtta
    14701 acggattgat ttttgacact ctttcacctc caaaaaataa gatcgcactg ttatcttaag
    14761 ctgataaaaa tgcaccgtta ttcagataac agtgcgatct tatcaaaaag cataactgag
    14821 aacaattctc aatgcaagct ttttttaatt aactcagtaa ttcaatccac ccaggaacag
    14881 aaaattgata caatcttctg gttaatagca tagtcttttc ttttgaatag ctatgcttca
    14941 atatatcata gaatgtagcg tagaatgagt aaatgtagaa gtggatttct tcttcattaa
    15001 agactaaatt ttgattgatc attccgatag tctttaactt atctagacaa cagactgtct
    15061 ttttcgtcac aaatgatgta attttttcaa caatatcttg atatttggtt ccagctgatt
    15121 ggaataaaat caaatcgaat atacgtttat tctcaaaaat ataattaata aatggatcta
    15181 attcattgaa gttctctctc caacttttct ctatatctat attttctaag tttaagaggt
    15241 atttggtaaa ctcttcatta tacatattaa taagcgcatt aatatgcggc tgaataattt
    15301 cttcaaataa cgcttcctta gaagtaaaat gtttataaaa agcacccgta gttacgcctg
    15361 cggtcaggca cagttgcctt atcttagttt gctgaaatcc ttctttttca aaatggctta
    15421 gtccactttc tattaacttt tggtgagtta taatataatc tctggtttct atgggtactt
    15481 cctcctaata agatgaatta actttattat cttacacagg acttgaagtt tcaatcgtta
    15541 acgtttatta ctgaactcat tccttagagc taaactaatg agaaagagca gaataactga
    15601 tacaaaaagc aatcctacaa gaattaatag actcatctaa atcattcacc ttttctaaaa
    15661 taatccgaga aagatttaaa aaaattatat ccagctatta ggataagtcc tatcataatc
    15721 actccaatag ccattttctt atcctcccta aaaataaagt cttttactta aaaccttttt
    15781 tgtaatcgtc aataaatttt ttccctttat cagttaattg aaatcctata tctaaatagt
    15841 cctcatatct aagtttagcc agcatctcac ttacagatgc tggactaaca cttagaactt
    15901 ttgctaaatc attatttttt aatttctttt ttctttttga taattcctga attgctagta
    15961 aataaattag tatagtagtc gttttaattt gtaccatacc taccctccaa attatttcga
    16021 aaggccttca gcaattttat ccaggttcca tttcatcatc gcatagtaac tatcaccatc
    16081 ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa tagataggaa tattggtatc
    16141 ttttgatact gttttcattg gtctatcatc cacactgctt tctacgaata aggagggaac
    16201 ttttgttgtg cgtaattttt caactaagtg ttttatttga tctggtgttc cttcttcttc
    16261 agtgttgatt tcccaaatgt aagcagaagg cacattatac gctttcgaga aatatttaaa
    16321 gcatccttca cttgtaacaa tcattttttt atcattcgga attgaagcaa atttagattt
    16381 agcttctttg tctagagaat ccaatttttc aatatactta tctagatttt ctttatagaa
    16441 ttttttatta tcaggatctt tttccgctaa ctgtttttca atatttttag cgtaaataat
    16501 accgttttct aaatttaacc aagcatgagg atcttccttc cctttctcac tctgaccctc
    16561 taagtaaata acatctatgc catcacttgc tgcaaaatag tctttgtttt cctctttgtt
    16621 cgcattttta actaattttg taaaccaagc atttcctcca gtttccaagt taacaccgtt
    16681 ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct tcaggcaaag gttcatattc
    16741 gtggggatct ttcccaatag gtacgatact gtgtaaatca attttatctt ttgctatatt
    16801 ttcggtaata tctgctaaaa tcgaattagt aactactact tttaattttt cgttcgaagc
    16861 cactttatct ttgtctttac tatttattga acatgccccg aatataaaaa gagcaatcaa
    16921 aatccccaga cttcccagta ctgctttcca ttttctcatc ataacgatct ctccttcttt
    16981 cttaaaaatt tttgttttgg tgagattaga aaactaatta caaataagaa tgccgctgtt
    17041 aaaacaatac ttgatcctgc tgctatgtta aaactataac caataaacaa tccaagcaat
    17101 gatgaacttg ctccgactgt tgcagaaata aatatcatag ttttcaagct atttgcatat
    17161 aaatatgctg tagctgctgg tgtaattaac attgcaacaa ttaatatagt tcccacactt
    17221 tgcatagctg ttactgctac taaagttaat agaaccatca atagataatg ataaaaactg
    17281 acattcatcc ctattgcttt tgccattaat ggatcaaaag aagtgattaa taattcttta
    17341 aaaaatagaa ctactataac caacactaaa accgaaacgc caattgtaat ccacttatca
    17401 atatcttgta cagctaaaat atttccaaat aaaatatgaa ataaatccgt tgagctattt
    17461 gcaactccta taaggataac tcctagtgct aaaaaagaac tgaaagtaat accaattgct
    17521 gtatctcctt ttattacact attgcttttt ataaaagtaa taatgataga agctaataga
    17581 ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa tataagaaat tgctactcct
    17641 ggcaaaacag catgagaaat agcatcaccc ataagagaca ttcctcttaa aataataaaa
    17701 caacctactg ttccagaaac aatccctata actacagatg taattagagc attctgtaaa
    17761 aagtgaaagt catacagacc attaataaaa ttttgaagca tattattccc cctctccaat
    17821 aaatatagta tctccatagg ctttttttaa attttcttca ttaaatacag attctacact
    17881 tccgtgagca attagatttc gattcaaaag aataatatta tcaaaatact tctttacctt
    17941 gcttaaatca tgatgcacaa ttagcactgt ttttccctga tgtttcaatt tttttaacgt
    18001 atccataatt attcgttcac taactaagtc tatccctaca aatggttcat ctaaaaatat
    18061 gaaatccgca ttttgagcaa gacatcttgc taaaagtaca cgttgaaatt gtcctccaga
    18121 taattctcct atttgatgat tagaatattt tcccatatcg accttttcca aagcctttga
    18181 aactctttgc cattctgcat ttttaattct ttgaaaaact ttcattccgg catatgttcc
    18241 catagataca cattctttta cttttattgg aaaagtaaaa tcaatatcag ctttttgctc
    18301 tacatatgca atcttcacca gttttttatt gatatcttca ttattaatgg agacctctcc
    18361 ctgatgaggg ataatattta acattgcttt aagcaatgtg gattttccag caccatttgg
    18421 accaataata cctgttaaag caccttcttg tatttttaaa gaaatctcgt tcaatgctaa
    18481 aaaatcgtta taacaaacat taagcttttt cacttctaac ataactttct ccttctaaat
    18541 gataatgatt atcacgatca tatggtatac aaaaattata ttttagtcaa ccctaaattt
    18601 tatagtttag atttttttct gttgctgaag attagaatga caaaactatt tgaaactacg
    18661 cgtatagtaa gtagctttca aaatatataa aaaaggagcg ttctcaaatg agaacatgct
    18721 cctaaaacta ctttattggc gtatggaaaa attatacttg aagtggatag cgggttgaat
    18781 aagttgataa gcatcaaaac atttataaaa attattcaag actatgtctg ttccatatac
    18841 aaatatgaaa atcagtaaac tccatttaac ttttttcgta gtgtgtcttt ttatacatgt
    18901 ttatcgttat taatagtgaa taggaaaaaa actatttttg atcattgaca attaaataca
    18961 atttttgaat aacgatccct gctattcgag ctagaaactc aagtacgcca tgacctgatt
    19021 agcgacaaat acttagagat ttctttttgt tctgggaaaa caaaatgcca tgacaatagc
    19081 agcaaataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg
    19141 tgcttaccca aagcaattca aaaatttcat aactactatt ttaggagatg attttataga
    19201 atccagagtc tacctgaata aaaaacaaac ctgtgcattt ctaggaatcg ataataatac
    19261 tctagataag tggattcgta tatataattt cccagtaata agagttggtg gtgtagttcg
    19321 ctttgacgta gaagatatta aagactttat gaatacttga aattttcttc tcgccgcata
    19381 tactaaaatt agtgaaatat actgccttac tttttttaag gagtgaggat tttgcctaca
    19441 aagctttcaa atgggaaata taaaactaat ctgagatatc caaaaaggtt taaagaaata
    19501 acaggaattg catcagaaaa gtatcaaaaa acatttccaa ataggcaatt ggcgattaaa
    19561 gccgaaaatg atatgaagaa gaaaatagaa aaagttttga gagaggagaa tgcaaactca
    19621 ttagaattaa aaggaaaaat aacgtttaaa aaattttacg aatctaaatg gcttcctaga
    19681 tatgaattag gtcaaacaat tagatcaaac aggcctccta gtgatataac cattagtaat
    19741 acaaaagata tatttcggtt gcatatttta cctatgtttg gtgaatacgc aatgaattat
    19801 ctaaatatca atactgagat aatatcagat gaattaacca aaaaatcgaa agaatatgca
    19861 aatataaaaa tcataaaagg atatgttcgt tccatgttcg atattgcaga aatactaaat
    19921 tatatcgaat ttaatcgaac tactaaaatt attcaaagta tcactgcacc taaaaaaaat
    19981 gctttagaag aaaaaagaat acaagaaggt aagcaagcac tatcatcaaa agaacttact
    20041 aattggatag aagcagtaaa tgatgatttt aataatcatt tacttacttt tcatgattac
    20101 acacttttta tgattaccct gtatcttggt gatagaaaaa gtgagactta cgctttgcaa
    20161 tggaaatata ttgattttga aaaacaaacg gtccgtctta aacatacttt agataaatat
    20221 caaagaaaaa agtttacaaa aggtcgaaaa gataccgtca ttcaagtacc agaagttgta
    20281 atgacattgc taagtgaatg gaaatcggta caagctgatc aattgttgaa attaaaaatt
    20341 aagcaaacgt tagatcagta tctctttact tatactaaac catctggaga agtaaattgt
    20401 cctgttcatg ccgattacct aaactatcga atcaattcaa ttaaaagaag gcatcctgac
    20461 ttagttcatt tatctccaca caaactccgt catacttatg caactatagc tagacaaggt
    20521 ggagcagata tgaatcaaat ttcaaatgct ttaacacact cagatatttc tacaacaaag
    20581 atatatgtga acacaccaga tatagttgat aaggctgttt ttgaagcttt tcagagagga
    20641 ttgaataagt gtgattgata tgtgttctta aatgtgttct taaaaacaaa agaagcactt
    20701 tccaaataat tgaaaagtgc cagaaacgtt gatatataaa ggatattaac gttttgagaa
    20761 ttgtgaagct ttacgtgctt tcttaagacc tggtttttta cgttcgacca tacgtgcgtc
    20821 acgagtaagt aagccagcac gtttaagagc taaacggaag tcaggatcaa cttctagcaa
    20881 tgcacgagcg ataccatgac ggatagctcc tgattgtcca gcgtatcctc cgccgtttac
    20941 gttaacaaat acgtcatatg cgccttttgt ttcagtaaca gcgaaaggtt gattgattac
    21001 ttcacgcaag tcagcatgtg gaatgtattc ttcaacatct tttttattta cagtaatttt
    21061 gccagttcct ggtactagac gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat
    21121 atattgaact tgtgccaatg aaattccctc ctattagatt aagtttgtga tgtctaaaac
    21181 ttctggttgt tgtgctgcat gtggatgttc tgctccaccg tatacgttca attttgtaaa
    21241 ttgtttgcgg cctaaagtgt ttttaggaag catacctttt acagaagttt cgatcaaacg
    21301 acgagaattt ttagcacgca attcgccagc agtgattgat ttcaatcctc ctgggtagtt
    21361 gctgtgacgg taataaattt tgtcagtcgc ttttttacct gttaatttca ctttgtctgc
    21421 attgatgacg atgacgaaat cgccagtatc caaatgaggt gtgaaagttg gtttattttt
    21481 accacgtagt acagatgcaa caactgttga aagacgtccc aatggaacat cagttgcgtc
    21541 tactacatac catttacgtt ctacttcgcc tggtttggcc atatatgttg tacgcacgtt
    21601 attttcctcc aatttaagtt ctgatgtttg acatacactt cgagtttccg gggctctttg
    21661 tggggcaaac aataccattt aacatattac ccgctaatct agtcaatgtc aacgtttttc
    21721 tgcttgattg aaaaatttat atgaaacttt acttcttttc tttcttttat ataaaagggt
    21781 cgtttcctgt caaaatgaaa aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct
    21841 tattttgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatccataa
    21901 aacataagaa gctgtttttt aaaattgttt cttgtgactc ggagctgaac ggcaggctta
    21961 caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    22021 aaatatgagg aactattttg acgtttgttt ctctaatact caggtcaaaa cgctgatttc
    22081 acaacctctt tttcggtcaa gttcaacaac agtttttacg cgtcgatctc tggcgctttg
    22141 cccaaatgag cttggatcat ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag
    22201 atatcttgtg ttggatcgtc gccttcttct ccagaaactt cgattccttt ttggtaaaga
    22261 tctgctagat aacgatagcc tgcaacaagt gtttccaaac gttctggaat agtacgatcc
    22321 cagcggccaa tttcatcagg gatttttgtg ttatctgcaa attcacgcaa tgtagaataa
    22381 ggtgaaccgt ctaatgtgat caagcgttcg gatacttcat ctaactgagc gttcaaatca
    22441 tccatgaact catccatcat tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa
    22501 tgtgtttggt gaacaacaac agacatttgg cttagatctg caactaattg atttaatact
    22561 tctttggttt gttggaattt catctttctt gcctcctaac attttcttac acttaaaata
    22621 atagtagact ttcaatgaaa agtacaacaa aatcatttac ctgagaacga ttttcagttc
    22681 gcttttttat ccgtaaagcc aatcattttt cagaatcagc taattcttct cgcagttgag
    22741 caaattgctg ataagtctgt gctaaatgct tttgtgtcga aacaaataat ggatagattt
    22801 tctgatattt tttatgatca gtttcttgta ctccaacttg ctctttcaat tctatcaatt
    22861 cctgtgcttc actgatactt tggatctttc cgatgcttct ccagcctaaa atcacagctc
    22921 ctaatgcgga tgcttctgtg acagtaggga aaacaagcgg gtaacccagt acatcagcca
    22981 tcatctgacg aaaaatcgga gattgagcaa acccgcctgt agcacggatc tctgtgagct
    23041 gattttcctg gcaaactcct tttaaaatcc gataaaggtt gagacagatc ccttctagaa
    23101 tcgccttcat catttctttt tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg
    23161 catctgcgtc ccaaagcggt gctcgctcac caagtaaata gggatgaaaa atcagaccat
    23221 tggcacccgc tggcacaaaa gcgatagccg aaagcagtgc atcatatgga ttttctcctt
    23281 tttctttagc agattgaacg acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc
    23341 cagcaccatt gctagtcgct cctcccacta cccaatgttt ctgatccaga acataacaaa
    23401 acgtctccat ttctggatgg aactgaggct gagtagtaat aaaacgaagt gctccgctcg
    23461 ttcctacggt caaagcggcg atcgtatcac caaaagcacc taaccctaga ttagaaagcg
    23521 gcccgtctgc ccctcccaaa acaaatgata tatcatttgg caaacctagt tctgctgctg
    23581 tttttgatgg tagacctgtt acttgatagg tggaaggaaa gacttgcggc aattgctcta
    23641 ttcgaatgtt caagtagtca agcacttctt ctgcccattg gaattcatgg atatcaaaat
    23701 atccagttcc tgaagctgtt ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga
    23761 tatagtcttt gataccgaca aaataagcag tttttttgaa taattcaggt tggctgtttt
    23821 gcagccactt gattttgttc aatggtgtca ttggatgaat cggcgtccca gtctgccgat
    23881 aaaattcttt gccaagcacg gtatatttca gtttttctgc ttcgtccgct gctcggttat
    23941 ctgcccaagt gatcattcga gataacggtg ttttctcttc atccaacaat atcaggccat
    24001 gcatcgcaga ggagaaggag acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta
    24061 cttcttttat aacggtgcag acaccttgaa agatcaattc ctgatcttgt tctgccatcc
    24121 cttgtgtttc ttggatcaaa gggtagcgaa aataagcgga tgctgcttct tgggcttcat
    24181 ctgtgaaagc aactgctttg gtttgtgtgg ttccgatatc taatccgatt gaaacgttca
    24241 ttttttcacc tcaaatttta tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt
    24301 tttgaaaagg gggagatggc gtgttacttt attttatcat tggttgctgt atcggatcat
    24361 ttttatgttt aaccgcccag cggattccgt taggtcactc tattatttat cctagatctc
    24421 attgtgtcaa atgctgtcgt tcgctttctt ggtatgaatt gattccgatt ctctcgatca
    24481 tcgtccaacg atttcgctgc cgctattgca ggtgtcggtt gcccttctac tatttgttgg
    24541 cagaagcctt atgtggtggt ctttttgcct ggttctttac tttttcttcc gcaagaaatt
    24601 tttcgacgtt catatggatg ctttcggctt tgctattttc gttgatggat ttattttact
    24661 tcatggtcca ttctcacacg ctattctgtt cttgggcaat tttatggatt ttttggttgc
    24721 aggccggcgt cttccaatgg caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc
    24781 ttcgctatgg tcaatcctat ctcggagctg gcgatactct gttgatgctt tcttggagtg
    24841 gtggcttgtc tctagaagag ttgcttcaga tcttgtttct tgctagtctg tttggaatat
    24901 ccgttttttc tgtttacttt atctgtcata aaaaaaagtt ggaaaagctg cctttcattc
    24961 cttttttgag cagtgcgttg ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga
    25021 caaacgactt gtcacaacct ctgatatata gtgttctctt accaaatcct tgtgacttgg
    25081 agctaggcga caggttcaca acctctccac taaatggtgt tcaatcagct gaccctcagt
    25141 attagctcaa aatgctggtt ttacaacctc tgatatacgg tgttccataa gtagcttttt
    25201 cggcttctta tttcttgacg ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt
    25261 ctcgataaag attgtaatgg agcttgtttt tcttgtaata atcctgatgg taatcttctg
    25321 caggataaaa ttcgcttctt ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc
    25381 tgtcttgcaa tgcttgtttt gaacgctctg caatatttcg ttcttcttca tcattatagt
    25441 agatcactgg acgataagaa tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat
    25501 cggtttgctg ccaatagatt tccaccaact gatcataaga aataacttct ggatcatatt
    25561 cgatctctac tgcttctgta tgccctgttg tgcctgttgt tacttgttca taagttggat
    25621 ttggtacatg cccgccagta taaccggatg tcacagaaat gatccctggc tgctcttcaa
    25681 atggtttgac catacaccaa aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa
    25741 atcccccttt tttctcatca taccttgatt tccaaaagga ggataacgtt ttgcttgtga
    25801 cagaaaaaac ggaaactatt atatacgtga tacggcttct gataaatgcc aaaacgaacg
    25861 tacttgatca aaaacggtta aatcaggaat cacatattca gcagaaggaa gatttatgtt
    25921 ttcacctatt cccaatacat gcatgttacc agccaaagct gcttgacaac cagcttctga
    25981 atcttcaatg accaagcatg catttggcgg tacattcaat tgctgtgctc ctttgagaaa
    26041 aacttctgga tcaggttttg cttttgatac ttgcgtgccg tcaataagga catcaaaata
    26101 acttgtcaaa ttcgtttttt ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc
    26161 gatcttgatg tttgtttttt tcaagtactc aagaacagct attgcaccag gcaaaacgga
    26221 tgtttcatcc attttttgaa tcgcttgaag atatagattg tttttttgct cagcaaaggc
    26281 tgatttctct ttttctgaga atttctgttc catttttcca aatgacagca atcgctctag
    26341 tgactcggtt ctggatattc ctttcagttg ctcgttttct ttctcagtga atggaatgga
    26401 cagctggtta gctaaaacca gccacgcttc gtaatgataa cgagctgtgt ctacaagaac
    26461 gccatctaaa tcgaatagtg ctgctttgat tttcatcgat tacctccttg aaaatgaacg
    26521 acttgaagta aagatgcgat gtttttagct gtgcgatgaa gattatcagt ggcttgctgt
    26581 aaagcttctt gtaaaggcac agctgcagga aggatcggaa aaacggccga aatgccaaca
    26641 tctgctgctt tttcgcttcc tttaccaaca cttccgcaaa tagctatcac aggaacgccc
    26701 tctggtactc ttctagcaat cccaactggc gcttttcctt ttagactttg tccatctaga
    26761 cgtccttcgc ctacgattac gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa
    26821 tccaatacgt agtcgatgcc agagactatt ttccctgaag caaatgctaa cagtcctgca
    26881 cccattccac caccagcact actgcccgat gcctcgatta cttgaggggc aaatctcata
    26941 taaaaatcat gcatggcttg gtcagccact gctaattcat tttctttcaa gcctttttgt
    27001 ggtccgaaaa caaaagaagc gccttctttt ccgcataaaa gattagtaac atcagtaatg
    27061 acagtgattt tggctttaat cagctcaata ggcacgttcg ttttatcaac tgtagcaatt
    27121 ttacctagat tttctcccag tgcttctact ttttgattat cttgatcgta aaaagcataa
    27181 cctagacctt ctgccatgcc gatgccgcca tcgttggtac tacttccgcc aacgccgatg
    27241 atgatgtgtc gaacaccttg ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg
    27301 gataacctaa gtggctgttg ttggtttagt gggacatctt tcaatccgac gatttctgcc
    27361 atttcaaaaa tcgcagtttg accgtctgaa gcgtactgga tttctttgtt tttgccaaat
    27421 gcattagtaa cgactgctgt ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc
    27481 gttccttctc ctccatcgcc tataggtagc agatcatatt cagcctgaac aaaaacttct
    27541 tgaaaacctg attgaatggc ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa
    27601 tctggcgcaa tcagaatacg cattattttc ccctccgttc caagatcagt tctttttcca
    27661 gctggtattg gtctccattg acaaaaatag gtaatgtttc tttcgtctct ttttttatta
    27721 ctatttgttt ttttgtcaaa acaaatagca acctgtgatt tttccaaata aaaggaaaag
    27781 aaattttttc ccatgctgaa ggaagtcttg gagaaataga aagagttcca ttcgttactg
    27841 taatacctga aaaaccaaat ataactgcta gccatagtgc tcctaatgaa gcagcatgaa
    27901 ttccgtcatc tgatgaatgc ggatctggtc ctaaatcaat caggcacgct tcttggaata
    27961 aatgatacgc ccattccatt tcttggatac ggcaagcctc tatggcgtga atcgctttac
    28021 tcaacgatga gtcgtggatt gttttgtttt catagtaatg caaattctta gaaacgattg
    28081 gttgagaaaa atactcaggt aataagtata acagcatgac aagatctgct tgcttgctga
    28141 cttggagttc atttacttcg ctacgggcat aatcaagtaa aatagactgg ctgccaggac
    28201 gttctttgta agtggtcaaa tcaataactg gcttactgag atacgtgtca tcttgcggaa
    28261 tcagctgttc tttgttaggc actggcaaat ataacttatc taaaaaattt tgacattctt
    28321 tcaaaaaaag cggatcgcca gcttgatatt taacagctaa ggccacatta taatgcgcca
    28381 gatagttcgt atatgcatta ttgtcgatat attctgtgta ttcatctgga ccaatgactc
    28441 tgtgtatctc taaatgatca tcgactttta cagctctgct cagccaaaaa gctgctgttt
    28501 catgaatgag cgtttttccc tttgtttcta aaaacgattg atcctctgtt gcttggacat
    28561 actcttgaac ggcataagca aggtctgcac tgatatgatg ttctgcggca gctgaagcaa
    28621 cttcttgtct tgttccggtt ttgatattca atgcagcaaa tttgggtgtc tcttcctgac
    28681 cagtgtgtgc gctttcccat gggaaaagtg caccttgata gtgataatct cttgcttttt
    28741 gccgtgcgcc ttctaatcga tcataacggt acagcagcaa attctttgct acttcaggaa
    28801 actgatataa aaagaaaggc aggataaaaa tttccgtatc ccagaaaaca tgccctttat
    28861 aaccttcgcc agttagtccc ttcgccccta cactaacact tggatcattg cggggagtca
    28921 taatctgtaa atggtaagca gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt
    28981 ggatcgttat ccgatgctga tgccaaaaat ctgtccaata agacatcgac tgctctaaaa
    29041 tttctgtata actaacgctt ttctgggctt gggcaatact tttcttaaac gggtctgttt
    29101 gctgaagaga ggtagagagc acgatgatct tctccagcgt aaaggacgta ttttttctga
    29161 tcttttctga aaaggaagcg gtgatccggc gattttttgc ttggaataca cctgctttat
    29221 tgaatttagc tgcaatccca atatcaattt tactttctgt tgtctggtag gatgcactga
    29281 tcaaatcgtc accaaatact cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg
    29341 tttgctgtcc atcgataccc gttttcacat ctacttggca gtcttcggtc aacggcttga
    29401 tgaccacttt cagggctgcc tgatggagat tattttgatc agcaacacga caagtaatga
    29461 cttccacttt ttttccttga gcagtttcaa tgagcgcata gcgtttaagc tctcccattt
    29521 ctaaatctag tgcccgctcg tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac
    29581 cgtcgatttt tatttcccat ctgatgacat ctgggagatt cactaactcg gctgattctg
    29641 ctcctaaagg tcggttatat acaccagctg caaacattcc ccttacttga ttccgatagt
    29701 cttcctcctg gcttcctctg ataccaaaaa atccatttcc aatggtcatc tgactcgcat
    29761 acttattgat atactctaaa tcaaactgtt tttcttctac tttcataagg taataacttc
    29821 tcctgtttct gccgaacgat ataaattttc caccagattc tggatgacat atccttgcca
    29881 gccatctgca atcatctgag tctggtcacc gagcgcccga ttcacaaaat gagccatact
    29941 tagttcatgt gggtcattaa ctggcgtggt cttttgtaag gaaaccagct ttccatctac
    30001 atcttcgaat agttcgcctg gatacagatt agcacctgcc tgatcgccac aaaaagccac
    30061 attcagtttg tcttctctga tattcaaagc aaacgaagta ttcagttgga tgatcttgcc
    30121 gttttctaat tccagtgtgc caaaaattgc atcttctact gtgaatttgc ttggatccca
    30181 tttcccgaat ttccctgaat tttttttcgt accaattttt tggaagctgt acgcccgaac
    30241 gctttttact ttaggaaatc cagtaatata catcgctgta tcaagcatgt gaatgccata
    30301 atcgatcaaa ggcccgcctc cttgaagttc tttgtcgata aacgttcccc agcctggaac
    30361 accagaccga cgaagcgcat tcgcttctac gtaataaata tccccgagaa catcaagatg
    30421 attcttgatc attgttgctt cgtcagaaaa acgatgctgg aatccataag ccaaaatctg
    30481 cccgttttta tcggctgtct ctgccatctt tcttgcttga tcagctgtca tagctggtgg
    30541 tttctcgcac atcactgaag cattagcttc gagggcagcc aatacatgtt cgaagtgaaa
    30601 gcgattgggt gtacagacac tcacgatatc gggagaaatc tcattcagca tttgtgtaac
    30661 tgatgtatac gcatgagaaa atcctgtacg tcggcaaaag tcttctgctc gctggctgac
    30721 tggatcaacc acagcggcca attctaagac atctgatctt ttttgataat aagcagcatg
    30781 tactttttct gctacttgtc cagcgcctat gattgctact ttcttcattt atttctccct
    30841 cctttgctta tttacttatt gttattcttt ctctattctt tctctaaatc atttaaatga
    30901 cgcgctgttt ccagacagtt tttcaagtat tgcaatgatt cctgataaat tttcacttca
    30961 ttttctcctc gcaaacgaca ttcatatacc atataacctt catactcatt ttgaagtagc
    31021 gtttgaaaat ggcgagtaaa atcaattgcg ccagatcctg gttggtagcg atgattatct
    31081 gctaaatgga tatgacccac atactctttt tgcgtagtca atgcttttga aatatcatct
    31141 tcttcaatat tcatatgata aaaatcagcg atgatctgca catgtttcaa ttgattagag
    31201 tcaatgtaat gacgcgcatc cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa
    31261 ggctccaaat aaataaccgt acctgtttct tcggctactt tatctaaata acgaagtgat
    31321 tcgctgactg ctttatagtc tccttctttg cttcggggtg aaaccatcgg cggtaatcga
    31381 taagtgaaca ttccccaagc tgctgggata acgattcctt tgcctccaac ctcgcttagt
    31441 gcacgcaaaa tctcagcaat ttgacgtaca ccattaagac gccgttcttc gataaagtca
    31501 ccgatccacc catcgtagcc gttacaagct gtcactactg ggatccctgt ttctttgatt
    31561 gcctgtttga ctgtgtcgat attttctatt aacaacttac cgtcgatttc gtattcttca
    31621 aatccacagt ctttgataaa tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga
    31681 tcttgcgtac cgaatttcat tgttctcctc ctcggacttg aacgcccatt ttgatacttt
    31741 cttctgggtg ctggtctaca taacgcatgt aagcttctgc gctttctgca aatggcacaa
    31801 ctggatcaat gatttcttca caatccaagt aaccgttcat caataattcc caacatgtgt
    31861 cctcgattcg tttacggttc catcttgggt aatctgggtt tggttcacta gatgctcgag
    31921 caaagacgat ttttgcatta ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa
    31981 aaggtttagc aaaggcaaca taagaaatga tcccgccata gccgatccct cttaaagaag
    32041 cttgtaatgc ggcagcactg ccactcgttt caataactga atctactcca cgtttatctg
    32101 tcaattcctt tagacgaaaa ccgacatctg ctttcatagg atctagcgtg taatcggcac
    32161 cgtatttttc tgctaattct ctacgctgcg ctatgggatc gatcccaacg atggtagtag
    32221 cacctgcttt tttagctaat tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg
    32281 ctacagcatc acctgctcga acatgtgctt cccggatacc acctaaagcg aattgagcag
    32341 gatcgtaaca tactgcattt ttccattgat ccaaccgatc aagtttacgt agtttataat
    32401 tatcaacacc gtttgtaatc agcgtttcac gaatgggtcc ataagtgcag actaactctc
    32461 ctttttgata ctctgtcacc aaactgcccg tttcttcgat cgtaccgaca atcatatttc
    32521 ctaattgaaa ttcgccaaat accacaccgc gttggcttcc ttcttctctt ggcaagaata
    32581 ggcgccattc ttctgaaaat tcttcatcga taaatggact taatcctcta aagtcgacaa
    32641 tttctgttcc tggttttgga gtagcgaaaa ttactcggat acgtacttcg ttcttttcga
    32701 tctcacggtc ttcgtaatct ttgaatccgg ctattcttgg ttctaatgca actaattttt
    32761 tcatcttttc tttcctcttt cttttctttt tatcctttta cgccgccttc gactaaatca
    32821 cctttaataa atcgttcaga taacgcatac ataatgatga ctggtaaagc ggtcaccaat
    32881 gaagcggcca tcattcgtcc ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg
    32941 attggtaaag taaatttttc ggaggtcgac aggaaaatcg aagcaaataa ataatcattc
    33001 cacgaaacca taaagcaata aacgaagaca gaaatgattc cagaaatagc caatggtata
    33061 atgatccgaa taatgatctg gatccggcta agaccatcga tcattgctgc ttcttcaata
    33121 tcagtcggaa tcgtgtcaaa ataacttttt agcatgaata tcgcagtagg aagtgtttga
    33181 acgaccatcg tgataatcag tgctgtattg gtatcataca aacctagacc ggaaatgatt
    33241 ttaaataatg gaacaacgat caaaataccg gaaaacatat aaacggtgta aaagctagca
    33301 ttgattttcg agcgagcttt gaagcgtagt tttgacaaag cgtatgcccc gctgatccca
    33361 atcactactg cagttacgga ggctatcaag gagatcacta aactattttt gaaatacatg
    33421 atgaatggaa aaatctcagc attgaaaata tcctgataat gttcaaaagt ccattctttc
    33481 ggaaaaatag tttgtgtcgt tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc
    33541 atgatcaaaa atggaaacaa attgacagct aatagcaaca aaatcgtcgc gtaaaataag
    33601 attttcctgc tctttttatt tttcatttca agtccacctt ctttcgtgag agcaagacga
    33661 ccgctgcaat aatgatgaac aatagaatcg atatagctgc tgcttttcca agatcattga
    33721 acgcaaaggc tgtctgatac aaataaacgc ctaagatatt gactttattc gtcagtagat
    33781 agacatctgt aaacatatag aacatccaga tagatcgtaa aacaatcaca gttgctagta
    33841 ctggtttgat tgccggcagt gtcacgatct tgaacttctg ccaagcattt gcaccgtcca
    33901 tttcagctgc ttcataaagc gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa
    33961 acgcatacgg atagtatctc cagatagcaa acaaaacgac cataaagaag ctagcaactg
    34021 gctggtcgaa ccacagcggt gcttcgttga aaagctgcaa cctatctact aacagatcat
    34081 tgacgattcc ataactatta ttgaacatat atttccaagc aaaaatcaaa gaaatagatg
    34141 gcgtaacata acttaataaa atcagtgaac gcaccgtttt cctaaatctg aacttgcggt
    34201 taagcaaaat agctacggct aatcctaaag ctgtactgcc tgctacaact aagaatgtgt
    34261 aagcaactgt taaacctaaa gaacgataaa attcactatc acttaaaatt tcaagataat
    34321 tgcttaatcc gataaaaacg gcgtctgagt taggattcag cggcatatcg aaaaaactga
    34381 tctgaatatt ggaaagcatt ggaatcagca ctaaaacagc taataaaagc atgcttggtg
    34441 ctaaaagtcc catggctaat tttaagtcag aatgtttcct tggacgattc attgaaacct
    34501 ccccctgata atctgccaat agctatctac ttttttcgaa caccattggc agaacttctt
    34561 gttatttcgt atttttttgt gcgtcagcta aagcttgatc gacatcttcg tttccgacag
    34621 ttacttggtt cactgctttt ccgatcacac ctgagcttgt gatatctccc attttcgtga
    34681 aattcttttc cccgactagt ccaaagactt gtacttcatc aaaagcacta gcaatctcag
    34741 atggcagctc gccaaaagac ttgatgactt cattttcttt gtaaccatcc agttcgacta
    34801 cttgtttatt cactggttga gcaccaccag gagacatcaa gacccacttt tccatgtttt
    34861 tcggttgaga taagaaagct gtgaatgctt cagcagcctc tttttgtttc tcgtctagac
    34921 cagaagaaat cgtcaatcca gatactgtgc catagacagc ttgatctttt tgtgttggga
    34981 tagcaaaacc gatattttct gagttccccg cttcataaac agaaggcaaa atgtaagttg
    35041 aatagattgc cattggtgct gttccattca tgaaagcatc tttgatttct gtcacatcat
    35101 tagagcctgg catcgtgtat tgggataagt tttgataaaa cgacaatgct tctctcatct
    35161 tttccgtatc gatcgttacc tcgccttcat catccaaaac atttgctcca tttgacaaag
    35221 caaattgaga gaacgcttgc tcactcattg ttccttcagc agtaggcata gcaattccat
    35281 attgtttatt ctctttatct gtgaaatgtt gagcaatttt taaaatatca tcccaatttt
    35341 ctggttctga aaaaccagct tctgataatt tttctttgtc ataccaaata ccttgaaccc
    35401 agccgctaat tggtgcagca atatagcttt ttccatcttc tgaacgaaca aggtttttgg
    35461 ctccgtcata ataattatct tctccagctt cctcgataac gttttggact gccgtctgat
    35521 caatcagttc atctttatcc atcactttag caaaatcctg actgacttcc attacttctg
    35581 gtaatttacc ggaacgagct aacgtaacga tttttgtatt gaacgctgat tcttctaccg
    35641 gcacttgctt gattttgatg tctggatttt ctttttcaaa atcagcaacc agttcattga
    35701 tcacacttaa tcgttcctgt tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg
    35761 attcgttaga gctttgactt tcaccacaag cagtaagaag aaatccagta gctagcagac
    35821 aggtagtaac aataattttt ttcatttttt tccacctctt attttattat tttcagccac
    35881 atatattcat acggctggaa tatcaacttt cgttcttgac tgcaatggat aatttgtcca
    35941 gaaaacaggt ctaggtaatc tccttttggt aaaagataac ctgctgattg attggaaaaa
    36001 ttatggataa ctaagacttt ctcttccttc gcttcgcgaa taaaactgat aaaagaatct
    36061 gctgagtcca gtaagtaaat caaactattc ggatggaagg ctttttcttt ttttcgaata
    36121 gatagcatct gggtcaattc accatatact gctgttctta atggatctgt ctgcaactct
    36181 ttctcaatat ctgctaacaa aaatttttgc cgattgattt ttcgattctc tcctgttttt
    36241 tctacgcctt tgtaatcatt tcgacttccc agcacacttt gaatataaat tgccggcacg
    36301 cctggtaacg ttaccaaaaa tgaatgagca gctaaaaagc gtttgcttct ccattccgtc
    36361 gtatctgttg gagaacttaa cgcatctaga tacgtagcgt ttatctcata tggactggtc
    36421 gttccatctg ggtttttctt gtagtttact cgggtaccct ctttcttcag ctgttccact
    36481 aattcaagga tcgttttttc cggcagaata cctcgtaccg gattcaatcc gataccgtca
    36541 tgggaggcta aaaaattaaa aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac
    36601 cattctttca acttacttgt attacccgaa cgaatcgctt gaagggtcaa aggtggtagc
    36661 gaaaactgat agaccatctg tgcttcattc tcgccattcc caaaataact gatattatct
    36721 ttgtgtggca catttgtctc agtgatcaat accgttcctg gagctgcttc atcagcgatt
    36781 gcacggaata attggatgat tttatgcgtt tctggcaggt gtatacaaga agttcctagt
    36841 tctttccata aaaatcccac agcgtccaag cgaataaatg aagcgccttt ttctaagtaa
    36901 aacaaaagaa tatcgatcat tttcaataaa acttctggat ttttaaaatt caagtctact
    36961 tgatcttcgc taaaagtcgt ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt
    37021 gtaagtaacg ggctggtcct tggtctagtc acgctgcttg tatctgtatc cagtggcaaa
    37081 tcaataaaaa aatctttgta cgtatcattc aagtttaaat attcctgaaa ccaagtactt
    37141 tcggcagaca tatgattgca gacaaagtcg aacattaacc gtgtttcgct tctcatttct
    37201 tctatatctt cccatgtgcc caaacggtcg ttgattgcct gataatttac aacggagaaa
    37261 ccatcatcag atgtatatgg gaaaaatggc aagaaatgta caactctaaa tgtatctgcc
    37321 aaatactttt gataaaattt cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat
    37381 tgatccccat aagtaatcaa gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg
    37441 cctttcttct gctgataaac actgactttt tgttcaatca tagaggcggt tttctcagac
    37501 aaatctgagt cgcgataaat cgcttgtaaa agttttgtat acatcggatt cccctttctt
    37561 ttttgggagc gttcccaacg atttgatcat attatggaaa acgcttactg tcaatagcat
    37621 ttttttaaat aattttgata tacacaaaaa tacaaataag aaagcttatg tatcaataat
    37681 taatagcttc ttaaaaaaat atggtctctc atgatcatgc aagctattcc taaattttca
    37741 aaatcataga caaaaaagaa tgtctattgt actatggaat tattctaaaa cgctcccaaa
    37801 aaggaggtgc atataaaatt gaaacctact atttatgaca ttgcgaaaaa agctaatgtc
    37861 tcaaaatcaa ctgtctccag agtattaaat aaccagccaa atatctctga taaagcaaaa
    37921 gcaagtgtac ttcaagcaat caaagaattg aattatgaac cgagcaaaat tgcaagagga
    37981 ctgtcaggaa atggttttga cgcgattcta gcattatctc atcgtacttc tcatacgacc
    38041 tatggcaatc cttttttttc agatgttatt caatccattt ctgccgtttc tgaacaaaat
    38101 aacttcgact tgattctaca aacggcaaaa acaccagaag aagagctaga aaaatgcgcg
    38161 aaaaagatcc aagaaaaaat gatccgagga attattattt taagctctac gatggatgaa
    38221 acacttcttt ttgaattgga tcaatacaat attcctattg tggtaatcgg aaaagtcgga
    38281 aacgactaca cgaacatcta ctcagtggac acggataatt tccaggatag ttacgatttg
    38341 gtggaaacac tgatcaagaa tggacaccgt catatcggct gtctccatgc tccgacagaa
    38401 gtttatgtat ccaatgatcg tctcaatggt taccgtcaat gcttatttga tcaccaccta
    38461 gatatccgta atgaatggat tatagacggc ggctttcgct tagaagatag catggcagca
    38521 atgaatgatt taatgaagct acgagaaaga ccaactgcga ttttttctac cgatgcatta
    38581 aaatcattga gtatttacaa agtggcagaa caatctggaa agcagatacc tgatgatctt
    38641 tcgatcgcag gattcagtga tcccttattg tcctccctat tttcgccaca gttgactcgg
    38701 atagacattc cgacaaatca gttaggaatc aaagccacag aacttttatt ccaactaatc
    38761 caccaccaat accctgaaga aaaaagaatc attctttcaa ccgagattac tctcacagat
    38821 tcgatccaac aacaagaaat gtagaacaga attataaaag aacagaaaca ctgaggataa
    38881 gtcagttact ctgttctttt ttcaatcact catccctcgt taccccctcc cggtatttta
    38941 gcgaattttt cttattttcc atctgtttta gatagctgtg gataactata atgtttgtgt
    39001 taaactattt tttccacagg gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt
    39061 tatagttcgt ttttttaaac acatttaccc ttatttcaat ccatgttatt tggggatact
    39121 tatctctaaa acataaatag aataacagac atgtctactg gttttgtttt attcgaggaa
    39181 ttattcaata atagctagca aaatattaaa aataaccgct ctatcttccc atcaatttat
    39241 tttgcattca aaaaatgatc tatctttact tttgttgatt gacttctctt ggaagatgat
    39301 cgttccgata ccttaattgg atacgaaccg atgttgttaa gatcctttcc cacttttctt
    39361 ctagttagac aaattatttt agacaaacca aaaaagaggt tgtgacaata atttgtcaca
    39421 acctcttttt taaaatatct attttgtttg attgttcatt gcgtcttctg cagccatttg
    39481 cgcttcaata tctgaaatac tgtagacatt ttcgcttgct acaccgactt cttttggttc
    39541 catgtcacga taacgttgca taccagtacc agctgggatg atcttaccta taataacatt
    39601 ttctttcaaa ccaagaagag gatctttctt accgcggatc gcagcatccg tcaagacacg
    39661 agttgtttct tggaatgatg cagcagataa gaaactattt gtttctaatg atgcttttgt
    39721 gatacctagt aagacaggtc gagaagttgc aggaacgcct cctgagacta gcgtattgta
    39781 gttttgatct ttaaagtctg cgatgtctaa taatgtacct ggtaagatat ctgtatcacc
    39841 tggatccatt acacgaactt tacgcagcat ttgacgaacc attacttcga tatgcttgtc
    39901 gccgatttct accccttgca tacggtaaac acgttgtact tcacgaagca agtagttttc
    39961 aacagataat acatcgcgaa cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa
    40021 tggtgcacca cggtggatca tatcgccttc tgctactttc atacgtgctg tataaggaac
    40081 agtatacgta cgtgtatctg tttttccttt gattgttact tctttggtac gtgttgcagg
    40141 atcttctgag atatcgatga catcccctgt tacttctgtg atcactgctt gccctttcgg
    40201 attacgtgct tcaaagattt cttggacacg aggaagacct tgagtgatat cgtctccggc
    40261 aaccccacct gtatggaatg tacgcatcgt caactgagta ccaggttcac cgattgattg
    40321 agcggcgatc gtaccaactg cttctccgac ttcaacgtca gagccagttg caaggttgcg
    40381 tccgtaacaa tgtttgcaga caccatgttt tgtattacat gtgaagacag aacggattgt
    40441 cactgtttcg acacctgcat caacgatttg ttttgcgatg tcttcggaaa tcaattggtt
    40501 tcctgcaatg atcaattcat ttgtttcagg atgacgtact tctttacggg tgtaacgtcc
    40561 cactaaacga tcttctaatg attcgatgat ttcatttccc tcgcggatgg cttggatttc
    40621 aagacctcgg tcagtaccgc aatcttcttc acggatgatt acatcttggg ccacgtcaac
    40681 cagacgacga gtcaagtaac ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt
    40741 acgagccccg tgagttgaga taaacatttc caagacggat agtccttcac ggaagttcga
    40801 aatgattggc aattccatga ttcgaccatt aggagcggcc atcaaaccac gcattccggc
    40861 aagctgtgta aagttggaaa tgttaccacg agctccagaa tcagacatca tgaagattgg
    40921 atttttcgct tccaaacctt ccatcagttt ttgttggatg ctgtcttttg cagcattcca
    40981 aacagcaatg acacgttcat aacgttcgtc atctgtgatc agaccacgac ggaattgttt
    41041 tgtaatagtt tcaacttgtt tatgggcatt ttcaatgatt tcttgttttt cattcagtac
    41101 actgatgtca gcgatcccta ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc
    41161 tttcatacgg tcaagcatct tagaagtttc tgttactttg aatcgtttga agacttctgc
    41221 gatgatgttt cctaagtttt tcttcttgaa cggtaatact aattcttgct ctttgatatg
    41281 tgcaggaata tcagtaccag cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt
    41341 gaagtctgtt ggttcattca gataagggaa ttcaggaggc atgatctcgt tgaagatgat
    41401 cttaccaact gttgtgatca tcgtacgttc tttttgccat tcagtaaatg gtttttcacc
    41461 taatgagtta gggttcacac cgatacgtga atgtaaatga acatatccgt tgcgccatgc
    41521 gataacggct tcgttttgat ctctgaagac cattccttcg ccttcgcgtc cttcttcttc
    41581 cattgtcaag tagtagttac ctagaaccat atcttgggaa ggagtaacaa ctggtttacc
    41641 atctttaggg ttcaagatgt tttgagcagc taacatcaac atacgtgctt ctgcttgtgc
    41701 ttcttcattc aatggaacgt ggacagccat ttggtctcca tcgaaatcgg cattataagc
    41761 ttcacataca agtggatgca aacggattgc gcgtccttgt actaaaactg gttcgaatgc
    41821 ttggatacca agtcggtgaa gcgtaggtgc ccggttcagc aatactgggt gttccttgat
    41881 tacgtcttct aaaacatccc atacttcgtc ttcttgacgt tcgattttac gtttcgcatt
    41941 tttgatgttt gaagctagtt cgcgttgaac aagctcacgc atcacgaatg gtttgaataa
    42001 ttcaatggcc atttcttttg gaagtccaca ttggtacatc ttcaagaaag gtccaacaac
    42061 gataacggaa cgacctgagt agtcgacacg tttacctagc aagttttgac ggaaacgacc
    42121 ttgtttccct ttcaacatat gagaaagaga tttcaatggg cggttacctg gtccagtaac
    42181 tggacgtccg cgacgaccgt tatcgatcaa tgcatctact gcttcttgca gcatacgttt
    42241 ttcattttga acgatgattc caggtgcatt caagtctaac aaacgtttca aacggttatt
    42301 acggttgatt acacgacggt aaagatcatt caagtcactt gttgcaaaac gtccaccttc
    42361 taattgaacc attgggcgta aatctggtgg aataacaggg ataacgtcca taaccatcca
    42421 gcttggttga tttcctgaag cgcggaatgc ttctaaaata tccaaacggc ggattgcacg
    42481 tgtacgtttt tgtccttgtg cagatttcaa ttcttctttc aattcggctg cttctgcatc
    42541 tagatcaaca ttatcaagta attgtttaat cgcttctgca cccatagcag cttggaattc
    42601 ttgtccatat tgttcacgtt tttcgcgata ttcgcgttct gtcaacaatt gttttttctc
    42661 taaattcgta tttcctggtt cgatgactac atatgaagca aaataaatga tttcttccaa
    42721 agcgcgtggg ctcatgtcta atacaagacc catacgtgaa ggaatccctt taaagtacca
    42781 gatatgtgaa acaggagctg ctaattcaat atgtcccatg cgttcacggc gaactttcga
    42841 acgagtaact tccactccgc atcgatcaca aacgattcct ttataacgga tacgtttgta
    42901 ttttccgcag gcacattccc agtcttttgt aggaccaaaa atccgctcac agaacaaccc
    42961 ttcgcgttca ggttttaagg tacgatagtt aatcgtctca ggttttttta cttcaccgta
    43021 agaccagctt ctgatttttt cgggagaagc caaacctatt tgcatacttt cgaatttatt
    43081 tacatcgatc aaaaggggtt ccctccattt catttaatgg caaaccgcaa gacggctgat
    43141 cttcgaccgc cttgcagctc atagaccgga ttagtctaaa ttatcttctg cggtttcaaa
    43201 gttttggatg acatcgtctt tttcatcttc cacttgttca gcagctttct tttctaattc
    43261 tttggcagtt tgctgttcgg caaattttgt caaagcatca acagtgatca aatcgtcgtc
    43321 ttcatcgtcc atgtcgcgaa gttcgatctc tgtttcttcg atatccaaga cacgcatgtc
    43381 cagacctagt gattgcaatt ctttcaccaa tacgcggaat gattccggta cacctggttt
    43441 tggaattggt tcacccttga cgattgcttc gtatgttttc acacgtccga ctacgtcatc
    43501 tgatttgtat gtcaagattt cttgtaaagt ataagccgca ccgtaagctt ccagtgccca
    43561 tacttccatt tcaccaaaac gttgtccacc aaattgagct ttacctccca atggttgttg
    43621 tgtaaccaat gagtaaggtc cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc
    43681 aagtttgatc atgtacatca caccgacaga cacacgtcca tcaaatggtt cacctgtacg
    43741 tccgtcgtac agaacagttt tcgcatcgct ggccataccg gcttcacgaa ctgtttccca
    43801 tacgtcttca tcacttgcac catcaaatac tggcgttgct acgtggatac ctaactgacg
    43861 tgcagccata cccaagtgta attccaatac ttgcccgatg ttcatacgtg aaggcacccc
    43921 taaaggattc agcatgatat cgattggtgt tccatcaggt aggaatggca tatcttcttc
    43981 cggcataata cgggatacaa cccctttatt tccgtgacga ccggccattt tatccccttc
    44041 atggatttta cgtttttgaa cgatataaac acgaactaac atgtttacgc ctggtgacaa
    44101 ttcatctcca gcttcacgag taaagatttt cacatcgtga acgataccgc cgccgccatg
    44161 tggtacacgt aatgatgtat cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa
    44221 tagacgttct tctgcagata attctgttac cccttttggc gtgactttcc ctacaagcaa
    44281 atcgccatct tggacttctg caccaatacg gatgataccc atttcgtcta aatcttttaa
    44341 tgcgtcttcc ccgacattag ggatttcgcg tgtgatttct tcaggtccta attttgtatc
    44401 gcgggcttct gattcatatt cttcaatatg gatagaagtg tatacgtcat cttttaccaa
    44461 acgacggctc atgatgatcg catcttcata gttgtaccct tcccaagtca tgaaaccaac
    44521 taaaacgttt tggcctagag ccatttcgcc ttgttccata gatggtccgt ctgccagagt
    44581 atcccctttt tcgactttct cacccaaatg aacgattgga cgttggttgt agcttgttcc
    44641 ggagtttgaa cgacggaatt ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg
    44701 tacgcggatt tcagaagcat caacgtattc aacgacaccg tcatgtttac ataacaaagc
    44761 agctcctgag tcatgcgctg atttgtattc cataccagta ccaacccatg gtgcttgagg
    44821 attgatcaac ggcactgctt gacgctgcat gttggcaccc atcaaggcac ggttggagtc
    44881 atcgttttcc aagaaaggaa tacatgctgt cgcaacggca actacttgtt taggtgatac
    44941 gtccatgtag tctactttat caatagatac ttctaagttt tcactttgag cacgagccat
    45001 aacaacgtct tgagcaaatg ttccatcttc attcaatggg ctgtttgctt gggctacgat
    45061 gtaatggtct tcgatatcag ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc
    45121 acggtctaca cgacgataag gtgtttcgat aaatccgaat ttatttactt tcgcataact
    45181 ggacaaacta ttgatcaacc cgatgtttgg gccttcagga gtttcgatcg gacacatacg
    45241 gccatagtgg gagtagtgaa cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc
    45301 gccaggtcct aaggctgaaa gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg
    45361 atccatgaac tgtgacaact gagaagaacc aaagaattct ttgatacttg ccacaactgg
    45421 acggatattg atcaattgtt gaggtgtcaa tgtttcagta tcttggatcg acattctttc
    45481 acgaaccaca cgttccatac gtgctagccc gatacggaat tggttttgta aaagttcacc
    45541 gactgaacgg atacgacgat ttcctaagtg gtcgatatca tcgacattgc cgataccttc
    45601 catcaagttc aagaaatagc tcattgaagc aatgatgtcg gctggacgaa ccgtttttac
    45661 agctgcttct ggatagccgt ttccgatcac gtttacttca cgttcagggt ctcttggtga
    45721 aaatactttg atgacttgga cagtcattgg atctgtgacc acaccgtctt ctgatggata
    45781 gtaagtcact gaatttaggc cattatcaat gaaaggagct agtgtttcca tgacttggtg
    45841 agtcagaact gttccttttt caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc
    45901 agctaatgtc agattcaata gacgtgtttt caaatctaat tttttgttta ctttgtaacg
    45961 accaacgttt gctaagtcgt aacgttttgg atcgaagaaa cgagcattca gcaagttacg
    46021 tgagctgtct gctgtttttg gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc
    46081 ttcttctgtt cttgagtcgc ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa
    46141 tgtttcgctg tcgccgaaga tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg
    46201 aactaatacg gttaaaggaa tcttacgtgt acggtcgata cgtacataag agatgtcttt
    46261 tgcatcagtt tccatttcta gccaagctcc acggttaggg ataacagtag aaccgaatcc
    46321 ttcttttccg tttttgtcga cttttccatg gaaatagaca cctggtgaac ggactaactg
    46381 agaaacgatt actcgctctg ccccgttgat gatgaatgtt ccttgttctg tcattaatgg
    46441 gaaatcaccg aagaatactt cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg
    46501 caatgttaca tgtaatggtg cagagtagtt tgcatcatgc gcacgagctt ctgctactgt
    46561 atactttggt tctttcaatt cataatctac aaattccaaa gatagattgc cattgaagtc
    46621 atcgattggc aaaatgtctt caaacatttc tctaagtccc tcatctaaga accattggta
    46681 agagtctgtt tgaatttcga tcaaatttgg taattccaac acttcactga ttcttgcgaa
    46741 acttctacgt tcgcgatgtt ttccgtattt tactacgtgt ccagccaagc tcttcacccc
    46801 tctaaaatgt cattcaaaaa gactacccga tcaattgtta caataatatt tcaaatatta
    46861 tttttaggta acaacagaca ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa
    46921 cattctcttg aaaccttact tcgtgagctt ctacttttgt ttccttatat taaagccgaa
    46981 acggacaata tttcgtttct gccttttctt catcagatag tttttgcaac ttcatatact
    47041 acacttattt tctataaaag tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt
    47101 ttcttaatgt tttcaatttt atgttttcaa ttatctgaca attcttaatt gacagacctt
    47161 tttatctcgg ttatacttta gaaaattcat tttgtggagg aacttctatg cgaaccaaca
    47221 tcaaccaaat cgcaccaaca caactgaata acttttactt tagctacttt agataggttt
    47281 gtattcatca tgatggatac aaacgttaaa cgacagtttg tatctatgat ggctaaagaa
    47341 ctttgtattg cagcaaaaag ccacgtagac aaaatgctaa gtggctgagc agtgaaaata
    47401 caaagtaaaa gtatcaagca ttctactttt tcaatttatt ttccgacttt tcaatccatt
    47461 tggacatttt gtctgaatgg attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa
    47521 aaaaaatata atcatcggat taggcgctgc cctgctacta gctttaggag gttgcgcaca
    47581 aaaagacaat tccgcagagg catcagatgg acaaaaaaca attggcgtat tgcaacttgt
    47641 acaacataac tcgttagatg ctgcctataa aggattcaaa gaaggattag cagaagaagg
    47701 gtataaagaa ggcgataatc taacaatcga ttatcaaaat gcccaaaaca atcaagacaa
    47761 cctgaaaagt atgagtgaga aactcgtcaa agaagatcct gatttgctac ttggaatcgc
    47821 cactccagca gcacaatctt tattaaatga aacaaccact atcccgatga ctgtcacagc
    47881 ggtcacagac ttggaacaag cccatttagt cacatccaat gaaaaacctg gcggcaatgt
    47941 gactggcacg acagatatgg tgccaatcga aaaacaaatc ggcttactgt taaatatcgt
    48001 gccagacgcc aaaacaatcg gcatcatgta taacaacggt gaagcaaact ctaagatcca
    48061 agctgatttg gcagaaaaag aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc
    48121 aaattccaca aatgatgtcc aacaagtgac gaaaagtcta gctaaagatg tagatggaat
    48181 ctatattcct actgataaca cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa
    48241 agaaacaaaa acacctgtcg ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc
    48301 aacagtagga atcgactacg aaagcttggg taaacaaaca gggatcatgg cagcaaaaat
    48361 cttagacggc aagaatccaa gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta
    48421 tgtcaataaa gacatggcca aagcattagg catcaatcca gacagcatca aagaagctga
    48481 ataaaacaca ggaggattct catgacaaat ttgctctctc ttttcttatc atctgcatcg
    48541 caaggggtct tatgggccct acttgcgatt ggtgtatacg tcactttccg tatcttggac
    48601 attgccgact tgactgctga aggaagcttt cctttaggtg cagggatcgc ggcagtaagt
    48661 attacaaatg gctatcatcc attgacagct tgtcttctag gctttttagg gggtgcggca
    48721 gcagggcttg tttccggact gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg
    48781 atcatcacca tgactggttt atattctgtc acatcccgtg tgatgggtgc gccgaacatc
    48841 tctttattgg gtcagccaac gatctttcat tgggtcgagt cattcggcat aacaaaagac
    48901 aacgctgtac tgatcgtcgg tctgcttttt gctttgatcg ttgtatgttt acttgttttg
    48961 ttcttacgta cagaaacagg acttgctatt cgtgcaactg gcgataatct ggcaatgagc
    49021 gaagcaaacg ggatcaacac agataatatg aagatcatcg gttatatgat ttctaatgga
    49081 tgcattgctc tttctggctc cttgcttgcg caaaacaacg gctttgctga tttaaattct
    49141 ggtattggaa cgatcgtaat tggacttgct tctattatca tcgcagaagt tttgttacgt
    49201 aaccagccac tatttctccg tctcttgact gtcgttttag gtgcggtcat ttatcgtttt
    49261 attttagcgt tagtcttcga actgaatgtt gaaccaagtg attcgaaact tgcttctgct
    49321 ttcgtgctag tgatctgtct ttctttcccg cagatccagc aaaaattcca tttacctcaa
    49381 aaaaagaaag gcggtagtgt caaatgaatg acgtcttaac attggaaaat ctccatcata
    49441 cctttgaaaa aggaacgatc aacgaaaatc atgtcctgaa aggcattaat ctttccttag
    49501 atcccggcga cttcgtgacg atcatcggtg gaaatggggc tggtaaatct actttgctga
    49561 acagcatcgc cggtacattt tctgtcgatc agggtcaaat tttattgaat ggaaaaaata
    49621 tcacgaaaaa atctgttgtc gaacgttcga aatccatcag tcgcgtattc caagatccta
    49681 aactagggac agccgttcgt ttaacggtag aagaaaatct tgctcttgct atgaaacgag
    49741 gaaaaaaacg cggcttcttc cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc
    49801 agctggttcg gctaaatttg gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt
    49861 ctggaggaca acgtcaagct attacgttat tgatggccac attacagcgt ccagaactga
    49921 tcttattaga tgaacacact gcagcgcttg accctgctac ttccaaaacg gtgatggaat
    49981 tgacagaaaa gctgatcact gagcagaatc tcactgcttt tatggtcact catgacatgg
    50041 aagacgcgat ccgctatggg agtcgactga tcatgctgca tcaaggaagg atcgtcgtag
    50101 atatccagca agaggaaaaa cagcagctca cagtgcccca attattagcg ttgttcaaac
    50161 aaaacagcgg cacggcatta aaagacgatc aagtactttt atcttcataa tcagaaagag
    50221 gatgggacaa aaaatgaacg cccaccctct tcttttttct ctttccttct ttcgtttttg
    50281 ctttatgtat caaccgttgt ttcttcgaaa actattttgt tctctccttc ttcctgatag
    50341 aatggatggg aaaggagctt gatgccatga caacgaaaga caaggtactt caattattga
    50401 aaaagacacc agattttctt tccggagaaa agttggcaca gcaactggaa gtttcccgga
    50461 caagtatttg gaaagccatc aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc
    50521 ctacagggta tcgttacctg ccctctgacg tactagatgc aaaagaaatc cagcaagagc
    50581 tacggtcgtt tattcctgat ttgaaagtac aaacgatgtt gacttctgaa tctacgatga
    50641 aagatgtcaa actggcagca gctcaaggag attctgtttc gaagttattt gttgctgata
    50701 cacaagaaca agctcgtgga cgttttggac ggcctttttt cgctgaacct ggacgaggaa
    50761 tctacatgag tctcttgctc catccagaca aacggtttga tgaattgcct caatatacga
    50821 tcatcgcagc cagtgcctgt gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga
    50881 tcaaatgggt caatgatatc tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag
    50941 cagtcagtga tatggaaagc ggcaggatca gtcatatcat cattgggatc gggcttaatt
    51001 tttctgttcc tcaaaatcat tttccagctg atctccaaga aaaagccatc tctgtctttc
    51061 ccgacggaca agctagtgtc acaaggaatc agctaatcag caaaatctgg caagaattgt
    51121 tcacttatct ttctcaccca gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat
    51181 tgggaaaaac cgtgtcgttc aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa
    51241 tcggtgatca tggcgaactg atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg
    51301 gcgaaatcag tctcacatct atccgtacgc aataattatc agatttcaca aaaggacacc
    51361 tgatctcata cagtgtcctt ttttctgaat tctcttcaaa agactctcct tattctatca
    51421 gctgacagtt tctctatcct ctagaaaaag gtattctaaa aatatctata aagcatggta
    51481 cactttcttt aaatgaatca atgcaaaaaa caagagagtc atcagtttac acgatcaaaa
    51541 gagatagcag gccgcttcct ttttcaatct tatagataga ccatgggtaa cgataaaaac
    51601 aaaaaagaac ttgcgactta ttttgccgta agttcttttt tagctagtgc cttaatctct
    51661 taatcccaac aaacgtctcg catagatcac ctgaccagag tgcatagcag catcatcaat
    51721 aatagaaacg agacgcattc ctcgtgtgac tggtggtgtc cagttctcgt caattacttc
    51781 atctagctcg gaaggttcca gccttcttaa gtaagaaata gcaaaatcag cagctcctcg
    51841 caagtatcct agcagatctt ctatttgatt agcttggatc tgctttgctt gttccagact
    51901 atgcttccaa tcttgttcgt tatctgctat cgtcatcgga aaccggcttt cccagtcttg
    51961 actatgccaa attggagaag ttcctgctaa atcagcaatc tggaaatcta attctcgagc
    52021 ggtatgccaa gccagccaag tcattgattt gatctgcggg gctctatctt ccactggaaa
    52081 acgattggcc tcctcaatag agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg
    52141 atccacactc tcgcatagca tatctaaata atccatttat tttcctcctt tttatttatc
    52201 ctacttactt tttcctaatt ctaggatacc tacttatatg ttactacaca cccttatttt
    52261 ttacacttta acagtcacca tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg
    52321 aactatcaat tgaacaagaa gcaacaatat gaacgtttca ttaatattct ttaaataaac
    52381 aatgattttt caaacgaaca gtttaagact aggagaaaaa gagtataata ggaaaatgtt
    52441 tgaaaggaaa ggaaggggaa aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt
    52501 gttgttcaat atcttttcgt atggtgtggc cgtttcagcg gaaacataca ttcctaaaga
    52561 ccctgggaat atttataatg atctaactgg taactatggc ttgttaggaa ttgctagtca
    52621 attccacgta tttgctaaaa ataagacaac tgtgaatgca catacagacg gaaatatagc
    52681 aacacatgaa cttgatgcaa aaaataattt tggtacagag cttcatgaag gtttattgaa
    52741 ccaagaaata aattatatac aaaaaactga ttctttcgct aattcttcag gcatacctac
    52801 aggtagcaca agaatgaata aatttgtcgt tggcagtaac gtggatgtag cacttcaaga
    52861 tggtcatcct acagtcaatg gatccaggct tgatcactta actccagatg atttttataa
    52921 agatcgtgct gggcatgaat atattgattt cgagcaggaa tttgctaaat tgaatgtagc
    52981 agcaaatgat cttgcaacga tcactccagc taaaacatat actgctgcag attttccaga
    53041 catgaacaat cggacaatcg atttaactga tttatcagat accgggtttt tattggtaaa
    53101 tatcgatgca gaagtgttaa cgatgaatac gccacttcaa atcattaatc caaatgatca
    53161 agtagttgta tttaatgtca tcaacagttc ttctgcattg aacgtacagt caccaatcaa
    53221 atacaatgat cgttcaaatc atgaaacaga agatttttcg gatgcgaata tctcttggaa
    53281 ttttggaaat gagatgactg atttgacaat cagtgctcct tttcaaggaa caatccttgc
    53341 gcctaatgct actatccgca ttactcaaaa ccaagacgga acgattatag gaacaaatgt
    53401 gattttagat gcagctacca atcgttggga tcctaacgaa atatttatca gcgacaatgg
    53461 tacagatacg acagacacaa gcgacagtac agatacatct accacaagtg atagctcaga
    53521 ttcatcaact tcaagcgaca gtacagatac tacctctacc a
//
LOCUS       DAZKOU010000009        49981 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000009 DAZKOU010000000
VERSION     DAZKOU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.009"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..171)
                     /locus_tag="ACG827_000473"
     CDS             complement(<1..171)
                     /locus_tag="ACG827_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0482643.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNY"
     gene            complement(173..370)
                     /locus_tag="ACG827_000474"
     CDS             complement(173..370)
                     /locus_tag="ACG827_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0482644.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(764..2491)
                     /locus_tag="ACG827_000475"
     CDS             complement(764..2491)
                     /locus_tag="ACG827_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0482645.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2484..3677)
                     /locus_tag="ACG827_000476"
     CDS             complement(2484..3677)
                     /locus_tag="ACG827_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0482646.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3864..4508)
                     /locus_tag="ACG827_000477"
     CDS             complement(3864..4508)
                     /locus_tag="ACG827_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0482647.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4602..4736)
                     /locus_tag="ACG827_000478"
     CDS             complement(4602..4736)
                     /locus_tag="ACG827_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0482648.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4738..6870)
                     /gene="feoB"
                     /locus_tag="ACG827_000479"
     CDS             complement(4738..6870)
                     /gene="feoB"
                     /locus_tag="ACG827_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0482649.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6867..7340)
                     /locus_tag="ACG827_000480"
     CDS             complement(6867..7340)
                     /locus_tag="ACG827_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0482650.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7741..7965
                     /gene="nrdH"
                     /locus_tag="ACG827_000481"
     CDS             7741..7965
                     /gene="nrdH"
                     /locus_tag="ACG827_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0482651.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8124..10283
                     /gene="nrdE"
                     /locus_tag="ACG827_000482"
     CDS             8124..10283
                     /gene="nrdE"
                     /locus_tag="ACG827_000482"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0482652.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10333..11298
                     /gene="nrdF"
                     /locus_tag="ACG827_000483"
     CDS             10333..11298
                     /gene="nrdF"
                     /locus_tag="ACG827_000483"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0482653.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11387..12526)
                     /locus_tag="ACG827_000484"
     CDS             complement(11387..12526)
                     /locus_tag="ACG827_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0482654.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12835..13548)
                     /locus_tag="ACG827_000485"
     CDS             complement(12835..13548)
                     /locus_tag="ACG827_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0482655.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13802..14503)
                     /gene="nagB"
                     /locus_tag="ACG827_000486"
     CDS             complement(13802..14503)
                     /gene="nagB"
                     /locus_tag="ACG827_000486"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0482656.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14737..16269
                     /locus_tag="ACG827_000487"
     CDS             14737..16269
                     /locus_tag="ACG827_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0482657.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16460..17662)
                     /locus_tag="ACG827_000488"
     CDS             complement(16460..17662)
                     /locus_tag="ACG827_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0482658.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17744..18433)
                     /locus_tag="ACG827_000489"
     CDS             complement(17744..18433)
                     /locus_tag="ACG827_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0482659.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18539..18907)
                     /gene="rplL"
                     /locus_tag="ACG827_000490"
     CDS             complement(18539..18907)
                     /gene="rplL"
                     /locus_tag="ACG827_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0482660.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18960..19463)
                     /gene="rplJ"
                     /locus_tag="ACG827_000491"
     CDS             complement(18960..19463)
                     /gene="rplJ"
                     /locus_tag="ACG827_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0482661.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19667..19794)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19873..20562)
                     /gene="rplA"
                     /locus_tag="ACG827_000492"
     CDS             complement(19873..20562)
                     /gene="rplA"
                     /locus_tag="ACG827_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0482662.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20666..21088)
                     /gene="rplK"
                     /locus_tag="ACG827_000493"
     CDS             complement(20666..21088)
                     /gene="rplK"
                     /locus_tag="ACG827_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0482663.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21431..22099
                     /gene="sdaAB"
                     /locus_tag="ACG827_000494"
     CDS             21431..22099
                     /gene="sdaAB"
                     /locus_tag="ACG827_000494"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0482664.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22112..22984
                     /gene="sdaAA"
                     /locus_tag="ACG827_000495"
     CDS             22112..22984
                     /gene="sdaAA"
                     /locus_tag="ACG827_000495"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0482665.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23075..24175)
                     /locus_tag="ACG827_000496"
                     /pseudo
     CDS             complement(23075..24175)
                     /locus_tag="ACG827_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24267..25154)
                     /locus_tag="ACG827_000497"
     CDS             complement(24267..25154)
                     /locus_tag="ACG827_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0482666.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25231..25776)
                     /gene="nusG"
                     /locus_tag="ACG827_000498"
     CDS             complement(25231..25776)
                     /gene="nusG"
                     /locus_tag="ACG827_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0482667.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25877..26047)
                     /gene="secE"
                     /locus_tag="ACG827_000499"
     CDS             complement(25877..26047)
                     /gene="secE"
                     /locus_tag="ACG827_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0482668.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26071..26223)
                     /gene="rpmG"
                     /locus_tag="ACG827_000500"
     CDS             complement(26071..26223)
                     /gene="rpmG"
                     /locus_tag="ACG827_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0482669.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26385..27029
                     /gene="cbpA"
                     /locus_tag="ACG827_000501"
     CDS             26385..27029
                     /gene="cbpA"
                     /locus_tag="ACG827_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0482670.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27085..27588)
                     /locus_tag="ACG827_000502"
     CDS             complement(27085..27588)
                     /locus_tag="ACG827_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0482671.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27601..27647)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28185..29150
                     /locus_tag="ACG827_000503"
     CDS             28185..29150
                     /locus_tag="ACG827_000503"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0482672.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29249..30421)
                     /locus_tag="ACG827_000504"
     CDS             complement(29249..30421)
                     /locus_tag="ACG827_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0482673.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30408..31553)
                     /locus_tag="ACG827_000505"
     CDS             complement(30408..31553)
                     /locus_tag="ACG827_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0482674.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31567..32491)
                     /locus_tag="ACG827_000506"
                     /pseudo
     CDS             complement(31567..32491)
                     /locus_tag="ACG827_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32608..33687
                     /locus_tag="ACG827_000507"
     CDS             32608..33687
                     /locus_tag="ACG827_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0482675.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33684..34312
                     /locus_tag="ACG827_000508"
                     /pseudo
     CDS             33684..34312
                     /locus_tag="ACG827_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(34422..34649)
                     /locus_tag="ACG827_000509"
     CDS             complement(34422..34649)
                     /locus_tag="ACG827_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0482676.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34752..37340)
                     /locus_tag="ACG827_000510"
     CDS             complement(34752..37340)
                     /locus_tag="ACG827_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0482677.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37360..38019)
                     /locus_tag="ACG827_000511"
     CDS             complement(37360..38019)
                     /locus_tag="ACG827_000511"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0482678.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38068..38571)
                     /gene="trmL"
                     /locus_tag="ACG827_000512"
     CDS             complement(38068..38571)
                     /gene="trmL"
                     /locus_tag="ACG827_000512"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0482679.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38792..39658)
                     /locus_tag="ACG827_000513"
     CDS             complement(38792..39658)
                     /locus_tag="ACG827_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0482680.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39767..40408
                     /locus_tag="ACG827_000514"
     CDS             39767..40408
                     /locus_tag="ACG827_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0482681.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40470..41831)
                     /gene="mgtE"
                     /locus_tag="ACG827_000515"
     CDS             complement(40470..41831)
                     /gene="mgtE"
                     /locus_tag="ACG827_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0482682.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41849..42748)
                     /locus_tag="ACG827_000516"
     CDS             complement(41849..42748)
                     /locus_tag="ACG827_000516"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0482683.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42750..43547)
                     /locus_tag="ACG827_000517"
     CDS             complement(42750..43547)
                     /locus_tag="ACG827_000517"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0482684.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43525..44229)
                     /locus_tag="ACG827_000518"
     CDS             complement(43525..44229)
                     /locus_tag="ACG827_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0482685.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44360..44936
                     /locus_tag="ACG827_000519"
                     /pseudo
     CDS             44360..44936
                     /locus_tag="ACG827_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            45054..45731
                     /locus_tag="ACG827_000520"
     CDS             45054..45731
                     /locus_tag="ACG827_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0482686.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45810..47618)
                     /gene="pepF"
                     /locus_tag="ACG827_000521"
     CDS             complement(45810..47618)
                     /gene="pepF"
                     /locus_tag="ACG827_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0482687.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47794..48792)
                     /locus_tag="ACG827_000522"
     CDS             complement(47794..48792)
                     /locus_tag="ACG827_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0482688.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49018..49671)
                     /locus_tag="ACG827_000523"
     CDS             complement(49018..49671)
                     /locus_tag="ACG827_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0482689.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14963 a        10488 c         8770 g        15760 t
ORIGIN      
        1 ataattcacc aatctattta ttaaaggacc gggatctaat tcatattttt gtttttctaa
       61 ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat ctttacttat
      121 agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca ttttagcact
      181 tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata caagtagttg
      241 ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt ccataatatt
      301 ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac aaaattttta
      361 aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt ctaacattca
      421 ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg aataagaatt
      481 taatcacttt taagtatata aactatgtat attctttaat attttttgag aatgcacttc
      541 gtacataata aactttaatc acattagtct ttcataccta cgatcgatct ctttgactcg
      601 cacccataaa ttcataatag tttgtcggcg tctttggacg tttcgaagat gagctacgca
      661 ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa atttggatat
      721 ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt ttccagcttt
      781 cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga tcttttccat
      841 tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa ttgtctaata
      901 ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta tggatctgct
      961 cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat ttggcaaaca
     1021 tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc gcttgaccta
     1081 gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc aagttgtcat
     1141 atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg attcctatga
     1201 ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg taggctggca
     1261 ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact tgatctttat
     1321 attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg gcatctgact
     1381 cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc gttacatcat
     1441 agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct gtatcccatt
     1501 caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc cctataccaa
     1561 tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt tttttacttt
     1621 tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc tgtgtaaaac
     1681 ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca ttcccgactt
     1741 gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg atcatagagg
     1801 caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc atgatcgtac
     1861 ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc ccgaatgaat
     1921 cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta aaacgaacgg
     1981 ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg ataccgaaaa
     2041 atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag tcaaggatcg
     2101 gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat ggtaccccaa
     2161 tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc aaatcattcc
     2221 atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta agtcctggac
     2281 gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa ggagctggaa
     2341 tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt tgtaaaaatg
     2401 aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc cagtttgcca
     2461 ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt ttctgctaat
     2521 gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc tacaactgca
     2581 agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat gtctttccca
     2641 atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt cctagcttct
     2701 aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac agccatcagc
     2761 atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc agattcgata
     2821 ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga acgatcgact
     2881 tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc ttcacctgtt
     2941 ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat ccgtaaagct
     3001 tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg caggtccagt
     3061 aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc ttcgtccatc
     3121 agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg ttgcatccca
     3181 ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt ttctaatgct
     3241 ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa cccgtattct
     3301 gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac catattgatt
     3361 ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc ttctccatca
     3421 atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat caatgtggat
     3481 ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac gtcaaaattc
     3541 acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt cggctggtga
     3601 tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt taaatgtttt
     3661 acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt ctttctgaaa
     3721 aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa agaaatttgc
     3781 ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta gccaagttaa
     3841 tattttttta atatttcaca caatcattct gcaagctgat gctccgggta aaaaaagttt
     3901 gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc tgccgtaaat
     3961 ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc gataactgtg
     4021 gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg cattgctttt
     4081 tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg cttcgtttga
     4141 ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg cttctccaca
     4201 cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata ttgcaatagg
     4261 aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg tacttccatg
     4321 attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga agcattgtac
     4381 gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg gttttcaacg
     4441 atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat ttttttcttt
     4501 ccagacattt tctttcctct ttcttttttt actttccctc atcttactat gctctagcaa
     4561 aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt gatctgttcc
     4621 ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt taaacgagta
     4681 tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt cgccatccta
     4741 ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg gctgacggaa
     4801 caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta acgctacctg
     4861 tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat aagctaaacc
     4921 gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc gatggatagc
     4981 cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg aataagctgc
     5041 tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt cggatacttc
     5101 cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga ccaatcctgt
     5161 aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa caactgccag
     5221 gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag cataaaaatt
     5281 ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc ctgctttttt
     5341 aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt gaggaaaatg
     5401 atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac gtgttttttt
     5461 caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag aaggagctac
     5521 ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca actttgcaga
     5581 acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt tttcaatcgt
     5641 gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag gaatgaagga
     5701 ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga tcctcgccat
     5761 atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt gcggtagaaa
     5821 tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct gcatccatcc
     5881 cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg taccaaacaa
     5941 tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca gataatagat
     6001 cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg caatctgatc
     6061 gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga gtgtacatag
     6121 ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt cgtccaatag
     6181 tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg ctatctcgta
     6241 atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc gtttcttgcc
     6301 gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac gtggatcata
     6361 tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct tttcgattag
     6421 tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac caagaccata
     6481 ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca tcatattcag
     6541 accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa gatttcgttc
     6601 taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa ccaaataatc
     6661 tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg gtaggtcctg
     6721 aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg ttacgcccgg
     6781 ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag ttttcccgct
     6841 gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat caagattagt
     6901 tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc gattggatct
     6961 cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc catatccatt
     7021 aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt ctctcctgct
     7081 tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt gacaaagatt
     7141 tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa aatggcattg
     7201 ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat attttgcaaa
     7261 tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa attctgcttt
     7321 gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg tattaaaatc
     7381 attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat cattctcaca
     7441 tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga gcgaatcttt
     7501 agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac aattttttct
     7561 ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt caaaaacaca
     7621 aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt tgactttttt
     7681 actaagagtt ttatactatc tattatagat aataagaatg tgttagagga ggcactcacc
     7741 atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt caaacggttt
     7801 ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc tgaagcagta
     7861 gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga tgcagaaaca
     7921 gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga ctaaaagaat
     7981 caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt cgcaaacttc
     8041 caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca tcatcaaata
     8101 cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc agttatttca
     8161 agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat aaggataaag
     8221 aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact tttctatcta
     8281 ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa ttcattcaat
     8341 tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa catttttctt
     8401 tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa accaacgacg
     8461 gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt tactttgcga
     8521 acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa cgatatcagc
     8581 cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg gtgtcttgtt
     8641 tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac tctgctttgc
     8701 aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt gaagccggcg
     8761 ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg aaattattcg
     8821 aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc gtggtttatt
     8881 tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa aatgcagatg
     8941 aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa ttttatgaat
     9001 tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa aaagaatatg
     9061 gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt gcaaatccaa
     9121 acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca aaattacaac
     9181 aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag aatccaatcg
     9241 atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa acgccttctg
     9301 tgattaacgg caaacaggaa tatgaagtat taggaacgga tatctcatgt aacttaggct
     9361 cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg cgtacgatga
     9421 ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca ccgatccaaa
     9481 acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac acttatcttg
     9541 caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat atttacttca
     9601 tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa agaaaacaag
     9661 ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa aaatacgtaa
     9721 ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc atttttatcc
     9781 caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga ctgtatcatc
     9841 aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat acgagtgcaa
     9901 gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc ggtaaaattt
     9961 attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct gcttatgata
    10021 tggacatgcg taaagtaatc gatgtttatg caatggcaca acaacacgtc gatcaaggca
    10081 tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa tggaagacaa
    10141 caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc cacaaaggaa
    10201 tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc ggaagcaatc
    10261 aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata ttttaattaa
    10321 aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga aattgaagat
    10381 atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga tacacgtatc
    10441 ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa agatacagta
    10501 ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag tggcatggat
    10561 cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa tattcaattc
    10621 atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa tacgaaaaaa
    10681 gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa aaaagctgag
    10741 cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc cagcgtcttt
    10801 ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt aggaaacaac
    10861 aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc tgtacatgga
    10921 acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc agaacaagaa
    10981 acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga agaacgttat
    11041 acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt cctacgctat
    11101 aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga tactgccaat
    11161 gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca tgacttcttc
    11221 tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga cgacgattat
    11281 ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa ataaaaagag
    11341 tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat tcacttgttt
    11401 cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt ctcaataaat
    11461 agattttccg gatatttaat attatcgtct taatcactgc atagacaggt atgcaaatca
    11521 gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg atcgtcaaag
    11581 gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca tctaattgtt
    11641 ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata aacaaaccga
    11701 caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata ttcgtgattc
    11761 cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt gccaaaaagc
    11821 tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca gataatgttt
    11881 cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga aagaagtgag
    11941 acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata aatggaacgg
    12001 taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca cttgcagata
    12061 aactgactaa tatgatattg aacaaattac taagagataa atttgctgat gctaattgtt
    12121 cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac catttgcttg
    12181 tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc attaactgag
    12241 gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac gtcaataaaa
    12301 aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga tcgaacatat
    12361 aatagataaa accagcaaca agcaatggta aaaaaactgc cgaaagcatt ttcaatattg
    12421 gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg agtactagct
    12481 ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat ctcctccaag
    12541 gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga ttttcatttg
    12601 cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact taaacaaaaa
    12661 cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat aaacggtgtt
    12721 caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga actattttta
    12781 tttggctggg cttattactt gatgtaggac atctatttta caacctcaga tcatttattg
    12841 cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact cctcgatcac
    12901 atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt acactcggta
    12961 gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt tctcttcaaa
    13021 ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt ctaacttttt
    13081 agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa tcccgataaa
    13141 actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc cagcaattgc
    13201 ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga tgatcgttcc
    13261 cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt catctgaacg
    13321 aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac tagcgatctc
    13381 aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg tacgcatacc
    13441 tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata gcatagcgat
    13501 ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct aaacaccctc
    13561 cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt tacctcttac
    13621 gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa cggtgttcaa
    13681 tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat ttttcctttg
    13741 tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct actattgaaa
    13801 attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg tgattttgca
    13861 atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg attgcatcag
    13921 ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt gaaccgattc
    13981 ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt ttgtttgcat
    14041 tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa gcaccaggtt
    14101 cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg attggatgtg
    14161 catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct tttccatctg
    14221 gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg aaatgacggt
    14281 agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca gaagtcattt
    14341 cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt gtgcttcctg
    14401 ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt tcaaatgctt
    14461 tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt gctcctttat
    14521 attttggtat agcccaatta tagcattctt tttttctttt tacaataatt agtatagacc
    14581 aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg tttcataaga
    14641 tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac tgtggacgct
    14701 ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac attaaacaac
    14761 attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca attaaatgct
    14821 ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt gattaaaatc
    14881 atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa tattcacaaa
    14941 ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga atttctaaaa
    15001 actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta ccaaacatat
    15061 agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac ggaattagac
    15121 caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg gttggggatc
    15181 gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg ttcaaaggtg
    15241 attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga accaaccaat
    15301 cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt tgacggtgcg
    15361 tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca tatcgcagac
    15421 atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt gaaacaaaag
    15481 gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca aatcgaaaag
    15541 accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc aaaaagccga
    15601 caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag taaagctgtc
    15661 tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac tgatttagtc
    15721 atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt tggtgaaaat
    15781 attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac actgatcggt
    15841 gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat caattacttt
    15901 tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt aagcagtcaa
    15961 tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg gttagttaat
    16021 caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa ggtgaaactt
    16081 gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac gaaccatatc
    16141 gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg aacagtaatc
    16201 atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat tgagattgaa
    16261 ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa gttttgtcac
    16321 aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta gctcaaaaaa
    16381 cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg cagaacagct
    16441 tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct ttaaaaacaa
    16501 ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt acacctaacc
    16561 agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct ccaataaagt
    16621 acataaacat aaatacagta ttatttcggt tgcttgctgc ttcacctagc ccctgtactc
    16681 gtgtttgatt ggcgacttga ccaaattgat tcccgacgtc taataaaata atagctaaaa
    16741 gcaaaatggc caaatggctt ccgccaacca ataaagcaat aaagcttatt gtttgcataa
    16801 caagtcccat atacacgatt tttctttcag aataagaatc tgccagtcgt cccacgatag
    16861 gtgcagcaac tgctccagac aaaccaaaaa tcgctaatat ccctgcttcg aatgttcccc
    16921 aattgtaggc gggactgctg atataaaaaa tcaacgtaga ccaaaagatc gaaaatgttc
    16981 caaacatgaa aaatccactg actgccgctt ctcttaatag acgttgagaa gcaactaaat
    17041 gcggcaaact tttaagtgaa tcaaggtatg aaacagctgt actactttgc tggacagaac
    17101 gcggcatttt ccagtaaagc agaagtgtaa gaattccgac tgcacataaa gcaaataaat
    17161 agatcatccg ccaagaaaaa agactggcaa caataccaga taccgtccga gaaagcaaaa
    17221 tccccacgag taaaccgcta agcaatgtac ccatgacctt ccctcttgct tttggtcccg
    17281 ctagtaccgc accgtaagga atgatgattt gcggcacgat cgaaagtaat cctattaaaa
    17341 aagaagcgca tgcaaataac gtaaacgaag gcgcaaaaaa agcggccaac agagagacgg
    17401 cagataacgc tgccattcga atgatcaatt ttggacgatc caccacgtcc cctaaaggaa
    17461 ctaaaaacag taaccctaac gcatagccta attgggtcaa catcgtcaat atcccgactg
    17521 cacttgtgct aacagataaa ctagctgcta cttttgttcc aatcggctgg atgtaataca
    17581 tattggcaac aacaacgcca caagtgattg ataatagtaa aataagcgaa gttgttaaag
    17641 gttttttttc attactcttc attgatttcc cccatttcct atcttagttt acaacttatg
    17701 gaaaagattg gaaatagaaa aatacaacta ttcgccacac ttattatttg tgcttttgtt
    17761 ttccttgttt tttcttgcga tctttataaa tgaagacaac taaccctact agcacagtaa
    17821 cacttgcaac caacgtgact gcagttttgt tatatccgtg aaacagagct tctccaccac
    17881 acgcatacaa cacagacgaa gggatgactc caatcacgat cgacaatatg atttgctgta
    17941 gaggcaattt catcgcatct gcagcaaaat tgaccataga agatggaata accggtatca
    18001 tatagcctaa cattacacct atttttggat gtttcatttg tcggattgct tgcacccaat
    18061 gattcgtttc ggttttctta tctaaaaatt ttaagcgatg cataaggaaa attgcagtga
    18121 tgttaccaag agcattaccc aaaatgttga tgatcgcacc tgtcagtggt ccaaagctca
    18181 aaccagcaat gactccaact actgaagtag gaatacctgg aacagcacac atcaccgctg
    18241 tcaatacgat caagagtatc gatgcaaaag tcccatgcga acgaatatgt gcgagtagct
    18301 ctgccttact cgctctcgga tttaaaaata attggatgtc tgtctgatat tctaaataca
    18361 accgatagcc cactaccagt atcagaatga ttcccaacga gatcaatatc aatcggatta
    18421 ttttcttttt catatttcct cctgaaaaaa atctatattc attaaataat agtttaacat
    18481 aaaaacaaaa aagcaccgaa atccaacata ggatctcagt gcttttaaat agtaaaaatt
    18541 atttaacagt tacagatgcg ccaacttctt caagtttagc tttcaattct tcagcttctt
    18601 ctttagatac gccttcttta actggtgcag gagcgccatc aactacagct ttagcttctt
    18661 tcaagcctaa accagttgct tcacgaactg ctttgataac tttaactttt tggtctccag
    18721 ctgcagttaa ttctacagtg aattctgttt gttcttcagc agcagcagcg ccaccagcag
    18781 cagctacagc tacaggagca gcagcagata cgccaaattc ttcttcaata gctttaacta
    18841 ggtcgttaag ttctaggata gttgcttctt ttaactcagc aacgatgttt tcaatgttca
    18901 atgccattat aatttcctcc gtttaattgt tcttttttat ttaatgcttt atgcggcaat
    18961 taagctgcat ctccatcttc tttgtctgcc actgctttga cagcgtaagc cacgttgcgg
    19021 actggcgctt gtagtacaga tagcaacata gatagaagtc cttcgcgatt tggcaatttt
    19081 gccaatgctg tgatttcttc caatgaagag actttacctt cgatgatacc gcctttgatt
    19141 tctaatgctt ttgcttcttt agcaaattcg tccatgattt tcgctggtgc gactacatct
    19201 tcgttactga atgcaacagc tgtaggtcca gtgaatactt cgtccatgcc ttcaaggcca
    19261 gcttttttag ctgcacgtga aagaatagaa tttttgataa ctttcatttc tacgttagca
    19321 tcacgaagtt gtttacgtaa acgtgttact tcatcaactg ttaatccgcg gtagtcaacg
    19381 actactactg atgcagctgc tgtaaatttt tcgcttactt catcaacgat tgctgctttt
    19441 tttgcgataa ttgcttcact catttttgag tttcacctcc tgaattttga tggaagagtt
    19501 ttttgcactg aaaaggcaag tcacttttat agttactttg ctcaaccagt attattcatc
    19561 acctattcac ttcgttcata gtgattctac tcgctttgct cgaaaggaaa ctcctttcgc
    19621 tttgctcaac cagtattgtt catcatctct tcacttcgtt catagtgatt cacaataaaa
    19681 aactctatgc caccgcagac atagagggac tattgaatat attcaataaa cgtcctcggt
    19741 aggaaattaa ggcttgatgc cacctactgt cttcggtaca agttaattat taacctcaac
    19801 tagcttagca tgctaaacta gttgagtcaa gaattatttt tcagttggat caatcaacat
    19861 tctaatattt aattagaaag aagcttggtc aacatggata ccaggtccaa atgttgttgt
    19921 gatcgttaca tttttcatgt attgaccttt agctgctgat ggtttagctt ttagcaatac
    19981 atcatggatt gctgcaaagt tttcaactaa tttttcatcg tcaaatgaaa cttttccgat
    20041 tggtacatgg atatttccag ctttgtccac acggtaagtt actttaccag ctttcacttc
    20101 gtttactgct tttgttacgt ccattgtaac tgtaccagtt ttagggtttg gcattaagcc
    20161 ttttggtcct aatactcgtc ctagacgacc tacagtagcc atcatatcag gtgttgcaac
    20221 aactacatca aagtcaaacc atccgttttg gattttttga accatgtcgt cgtcacctac
    20281 gaaatctgca ccagctgctt cagcttcttt tgctttgtct cctttagcaa aaactaaaac
    20341 agtttgtgtt ttaccagttc cgtttggtaa tacaacagca ccacggattt gttggtctgc
    20401 ttttttaggg tctacgttca aacggtatgc aacttcaact gttgcatcaa attttgcaat
    20461 gtttgtttct tttgctaaag ctactgcttc ggcaacagga taaactttac ttgaatcaac
    20521 tttttttaat gcatcttgca tttttttgct ctttttagcc attttggttc ctccttgatt
    20581 gtggttataa cggttgtacc tcccacgtgt tctatatctt tcaatataga gccaactgag
    20641 aagctgctgt cttagggtgt atagattatt ctacagtgat ccccatgctt cgtgcagttc
    20701 cttcaaccat gcgcattgct gcttcaacgt tagctgcgtt taggtcttcc atttttaatt
    20761 cagcgatttc tcttacttga tcgctagaaa ctttcgcaac tttatttttg tttggttcgc
    20821 ctgaaccttt ttcgatttta gctgcttttt tcaatagaac agcagctggc ggtgtttttg
    20881 taacgaatgt gaatgaacgg tcttcgtata cagaaataac aaccggaatg atcaaacctg
    20941 cttgatcagc tgtacgagcg ttgaattctt ttgtgaatcc catgatattg atacccgctt
    21001 gacctagtgc aggacctact ggtggagctg gagttgcttt acctgcagga atttgcaatt
    21061 taacgatttt ttctactttc tttgccacga gacatacctc cttaagtccg tgatgtggtt
    21121 aattggagat gcatatttct cctcccacgt tttatctgat ctgaaaaaat ccagacaaat
    21181 aatgtgcaaa acaatgcaca ctggtacagt atagcaaatt tatttgtttt ttcaagattt
    21241 ttttctgcta tttttacatt ttgcttgaat aaacgctaaa tttattctaa aaaaacaaca
    21301 atttttcgtt ctcatttctt cttttatgtt acacttgcgc tagcatggtt caacagatga
    21361 aagcaaacaa taacacggtt ttttagaccc ttgtacaaag attcctacat aacaaaatga
    21421 ggtggagaaa atgaaagatt taaggtatag aagtgtcttt gatattattg gacctgtgat
    21481 gattggtcca agtagctctc acactgccgg agctgctaga atcgggaaaa tcatacgtag
    21541 tatatttggc gaacagcctg attctgtaga tatttatcta tatgaatcat ttgcaaaaac
    21601 ctatcggggg cacggaacag atattgcatt agttggcggc ctgttaggga tggaacccga
    21661 tgatgaacgt ttagctgatt ctttgaaatt ggcatatgaa gccggtatgg aagtcttgtt
    21721 tgttcctaag agtgaaaaag ccgaacatcc aaactctgta aagatgcttc taaaaaaagg
    21781 aacacgaaag ctttcagtca ctggcatttc gatcggcggt ggcaatatcc agatttctga
    21841 attgaatgga ttcaaaattt ctttaagcat gggtactcct accttgatcg ttgtccacca
    21901 agatgttcca ggtatgattg ctaaagtgac tagtatactt tctgagacca atacgaatat
    21961 cggcactatg acagtgacac gggaatcaaa aggtgaaaat gccattatga taattgaagt
    22021 agatgatcct caagtagaag aaacggtaaa aagactcaaa cagctgccaa atatcgacag
    22081 cgtaaattat ttcgattaag gagctaaaaa aatgttttta tcgattgaag aattagtaga
    22141 tcaagcaaaa gcttatccaa gtgtagcaga attgatgatt gcagttgaaa tggaaatgag
    22201 tggccgttcc cgtagtcaca tcatcgagat catggaaaaa aacctctcag taatggaaca
    22261 atcaatcaac gagggaaaag aaggtgttac ctccgtcaca ggaattaccg gtggtgatgc
    22321 aaaaaaatta gatgattatt taaatgaggc agacttttta agtggagaaa caatcttaaa
    22381 agctgtacgc aatgctgtag ctgttaatga agtcaatgca aaaatggggt tgatttgtgc
    22441 tacaccgact gccggaagcg cgggtgttgt tcctggtgtg ctgatggctg tccgagatcg
    22501 gcttgatttg acacatgaac agcaattgga attcttattt accgctggcg ctttcgggtt
    22561 agtcatcgca aacaacgctt ctatcagtgg tgcagaaggg ggatgtcaag cagaagttgg
    22621 ttctgcaagt gcaatggcag cagcagcttt ggtttgtgca aaaaatggga ctcctcatca
    22681 agcagcacaa gcagtagcca ttacattgaa aaatatgatg ggactgattt gcgatcccgt
    22741 tgcaggctta gttgaagtcc cgtgtgtcaa acgtaatgct ttaggctctt ctcaagcttt
    22801 tatctctgct gatatggcat tagcgggtat cgaaagtgtc attccgccgg atgaagtcgt
    22861 tgctgcaatg taccaagtcg gacgtcaaat gccgtcgatt ttcaaagaaa cagcagaagg
    22921 tggcttagct gttacgccaa ctgctaaacg acttgcggaa gagattttaa ataagagggc
    22981 gtgataaagc tccttaattt cgagtattaa gagattagtt atcttcataa caaaaaacga
    23041 agagaggact aattgccctt ctcttcgttt tttattaatc catcaatttc gtatatttta
    23101 cttcatcgat cgtttttgtt cctgcaagtt gcatcgtgat cgacaattct ttgttcaggt
    23161 gttcaaaaac agatgttact ccttctgccc cacctagatt caacccataa atcactggtc
    23221 ggccaatcgc aactaagtca gcaccacttg ccagagcttt gaagacatgc tctcctcgac
    23281 ggacaccaga gtcgaaaatc actggtacac gtttgttgac tacttcggca atttttggca
    23341 atacttcaaa ggacgcagga cctccatcta gttgacgtcc accatgattg gatacccaga
    23401 taccatctgc ccctgcagag atagcgatta ctgcatcttc tggcgattgt attcctttta
    23461 cgataactgg aagatgggtg atttctttga ttgttttgat gtcatctggt gtcaagcctt
    23521 gttttgcagc tgcataaatt tctgcgattc cttttccttc gccattacca cttgcacttt
    23581 gttcgctata cgcagctaag ttaggcattg gtaatggaaa ttggaattga ttgatgacgt
    23641 cttcttcacg gtaaccaccg agcgttgaat ctgccgttaa aatgatagct ttagcccctg
    23701 cttctactgc tttgttcaaa atgaactcat tgaatctgtc gtctttactc atatataatt
    23761 ggaaaaactg cggtgcgcct ggcgctgcat cggctgcgtc tttgattgtc gtatttgcat
    23821 aagtgctgat ggaaaagatt gatccagcag cagctactcc tttagctgta tcagcttccc
    23881 ctttttcatg tgccaatcct tgggccgctg atggcgcttg gataatcggt gtatctaatt
    23941 caattccgaa aacagatgta tgcaaatcgg ctgagtcgat tccgcgtaat atccgtggca
    24001 tgattttctt tgtattaaaa gaagtcgtat tttctttcat cgtccactca tcttcagaac
    24061 caccgcgaat atagccaaaa gctcctgctt ccatgcgctc ttttacacga ccttctagtt
    24121 aggcaatgtt tacaatatcg attggatgtt cttcattact tgtatgataa gtcatagtag
    24181 atccttcttt catcttcttg attacatata aatcatacta aacttacaaa aagtaagcaa
    24241 acatttactc tcagatactt ttatcgctat tctttccaaa taaatcgact tacaagctga
    24301 tctacttctt ggttttcatg aagcatacta tgagaggcat tttcacctgt cactactttt
    24361 tcctctattt catttccgcg ttgcttcaac aacgcataga ccgctaatgc actgtttaac
    24421 ggtactgttc catcactcaa gtctgtttca tctagttgac cagcaatcaa gaaaaatcga
    24481 gtggttatag gaatattacc gatcatttgc tggtaatctt gatagcggtt agaaacgaca
    24541 gcaggcccct ttgctaactc atctgtcaga gactgcgcac tatcatcgac aaaatcatta
    24601 aaaggcgcgc caattgctgt taatgtctga acttgtggca atgacgtatc ctgtccatac
    24661 gttcctaaat agcgtagtgc actaactcct cccatggaat gccccactat gttgacttta
    24721 tctatgccgt agttttcttt cagatacaaa agacaagttt tgatccattc tgcttgattc
    24781 cactcgttat ttttattatc ttcaaacaat acttggacca tcggattgtc tgcttttcca
    24841 gacaaatctc ctgttgcttg tatttgtcca tctgttccta ctgttaaaac caattctttt
    24901 actgcttgct gatttttttc caaacgccgc aacatccctt taaaggaatt ttctgtcccg
    24961 ctgtagccgt ggaaaaaaag tgttggaata gtggtttttt ctatcacttt actggattct
    25021 gccgtttttt ctttatcaga aacttcagta gtatttatct tcgtcgtctg gcaaccactt
    25081 aacgcaaaca aactgataag gactaaagct gctgcagttg cacgtttgca taaatttttg
    25141 attggttctg tcatacacct cttcctttcc atatagaaaa aagaaagact tgaaaggcca
    25201 atcatatgat cgacatttca agtctcttta ttaatcgatt ttatctactt gatcgaaatc
    25261 aagttctgta ctggtttcac gtccaaacat atcgatgttg actttcaatt tttctttttc
    25321 tccgtcaact tctgttactt gtccttctaa tccagcaaat gcgccttcga tgattttcac
    25381 gacttcgcca acttctactt ctagatcatt ttgtcgcagg ctcataccga tagaacgcaa
    25441 gatatgattg acttcttctg gaagtaatgg tgctggtttg cttccggctc cgtgtgaacc
    25501 aacgaatcct gtcacaccag gtgtgttgcg gacaacatac catgaagcgt ctgtcatgat
    25561 catttcaaca agtacgtatc ctgggaatgt tttatgaacg atttcttttt ctttcccatt
    25621 tttgacttca gtttcttttt cttcaggaac aacgacacgg aaaataaagt cggccatttt
    25681 catactttgc gcacgagatt cgatatttgc ttttactttg ttttcatatc ctgaatatgt
    25741 atgcaagaca taccagtttt tttcaaaaga ttccattgaa ggctcctcat ttcattgtaa
    25801 aataaaaaaa cctcagtaca cccgaagttt ttctttcgct tcattatagc actaaaagta
    25861 cgttgtggct agcgagttat tttaaaatcc agccaaataa gctttggatg gctgtatcca
    25921 ttacgtaaaa catcaatccg aaaataatag aagtttcgat gactactaac gtatctttac
    25981 ggagttgttt tctgcttggc caagttactt gtttgatttc atcttttacg ctttttagaa
    26041 atttcatgag acaacctccc ttgctactgt ttatttcgtt tctttatgta atgtatattg
    26101 attacaatat ttacaaaact tattgatttc caaacgttcg ccgctttttc cttcactgac
    26161 tgattttgtg tagttgcgag acccacaaac ggaacaagcg agtgctgctt ttttctttgc
    26221 catttacttc tgcccccttg ctgagaatca gacttatact tcataacagt atcacgtaac
    26281 tttttacttg tcaatttgtg aaataaacta tctattgttt tggataaaat tatgatatga
    26341 ttaatttgaa attaagctaa aatgagacaa ttggaggtat agcgatgcta ttaaaaacac
    26401 ttgtatataa gaaaaaagat ctgacaaccg ttacagagac tagtacatta gaagaagcat
    26461 taaaaatttt agaaacttcc ggataccgtt gtgtgccgat cttagattct tcaggtaata
    26521 ttttccgtgg aaacatttac aaaatgcata tctatcgtca taaagcaaat ggcggtgata
    26581 tgacgcttcc tgtcactcat cttttgaaaa acgctacaaa gttcatttac ctggatactt
    26641 ctttcttcaa agtattcttt acgatcaaag agcttcctta tattgctgtc cttgacagtg
    26701 aaaatcattt ttatggtatt ttgacacata gtacgttgct taatatgctc tctcaatctt
    26761 ggagcgtaga acaaggaagc tatgtactga caattgcttc cacaggaaag caaggagatt
    26821 tggctgctat tgcaaaaatc atttcaaaac acagcagcat tgccagctgt attactcttg
    26881 atatcgaaaa agacgaatac attcgacgta cattgatcac gctaccggct aacactgaag
    26941 cgaaagtatg tgaaacgatc gtcaaccact tagagaaaaa gaattataaa gtggtagaaa
    27001 ttgaaaattt gcaaaaccaa gaagcataat caaaaagcga aaaaagaaaa cagctaatct
    27061 gctttctttt ttcgcttttt ataattagac acgcttttca aaatcaagcg cgcgatctaa
    27121 ataatacatc aatggtgctg aaacagccat gacgataaac tcacttaatg caagcgtgcc
    27181 ataggttgtc caaaatgctt ggccacctgt tggaactaac atataagcaa tcagtcccat
    27241 gctgatggta aagaagatcg tgttcaatac taaacgcatc tttacatttt tcactttgtt
    27301 ttgcaataaa gctgtcaggc ctaaagaaag caatgtttgg ccgccaccaa aaaccacatc
    27361 catgatacca aaaccaaaga aggcattata gacgatgacc ccgcctaaaa ctccccaaag
    27421 caatttacgg ttaaagacaa ctaaatgatt caaactttct gaaatacgaa actggatcgg
    27481 ccctgaggat acaggcgcaa ctaaaatagt taacgctaga taaagagcca tgatgatgcc
    27541 atttacaacg attacggata accgttgtct gcttttcgtt ttttgcattt tctctcctct
    27601 tttctcctag tttttttaac gcgggatggt tgatgaaccg cggttatgtg aaacaaacaa
    27661 taattataac agacttttca tttatttcaa tccgtcttat ctgcttttaa aaaagcaaaa
    27721 gcttaaacaa tgtaaacgtt tgctaaaaat cgtgggatat ggtataaagt aagtgtaagg
    27781 ggtcataaga attcccagta aagcagacga agtaggatct gcaagaacag aattgttgtg
    27841 aaacttaaat aaataaaaaa ggatgtggga atttgagcaa cctcttacta gagaacggtc
    27901 tagcaagcag ctgaagatct aagtggacat gccgaacctt ggaacttctc cttacgtaag
    27961 taatggatca tgcgcttgct aggctttttt gtgtttttcg gtcttcggac ctttttcatt
    28021 ttcctagact tctgctataa taaccaagaa ttaattttgt aacagatttt tttgaaaaaa
    28081 caagtttctt tcattcaaaa aatagaaaaa gcttgctaat tattggttaa ccagcaaaca
    28141 ggagtattaa aatatcttgt tttattgaat agcaggagga aatcatgaaa ttccattatc
    28201 atatattgat caatccatcg gcaggcagcg gaaatggcta taaagtagca gaaagaattt
    28261 taccggtatt aaaaaataag catattgact atacacttca ttatagtgaa tacaaaggac
    28321 acgatgctga gattgctgaa actttggcaa aagaaacatt gattccttgg atagaagaag
    28381 aacagaaaac aatcgatact ttcccccttt tgatcatcgt aggaggtgac ggcaccttac
    28441 atcaggtact tgatactttc tatcagatgg aagtagaatt cccagttgct tatattccag
    28501 caggttctgg agatgatttt gctcgagggg caggccttcc aaaaaatccc aaaaaaggac
    28561 ttcagctgat tttagcagct caatcaccag aaaaagtcca cataatggct tatgaagaaa
    28621 aaataagtga aaaaaaagga atagctgtta ataatttcgg tataggatta gatgcagcta
    28681 tcgtccatgc gacaaatcat tcttccacaa aaaagcgatt gaacaaatac aacttaggat
    28741 cattttcgta tttattctct attctccgtg ctttattcac tcaaaaagga tttccaattc
    28801 tggtagatgc aggaggaaaa caattaagtt tttcaaacgc atttttatgt acagcaacga
    28861 agcatccttt ttttggcgga ggaatcgcga ttgttccaac tgctgatgcg tctaaaccag
    28921 tcattgattt tgtcgtggtc gaacgaatca atttctttaa aatcttgtgg ctcattcttt
    28981 tacttataca gaaaaaacaa ttgaaatcga aatatttcca tcactacaca acaaacagat
    29041 tacgtatcgt ttcaacaaca ccgcaacatg gtcaagaaga tggagaagaa atggaaaaac
    29101 gaccttttga tatcacaata accacaacta aacagttgtt ttggtgttaa taaaagaggt
    29161 tttgtcagaa gctacatctg gaacaccgtt tattcggaaa aaaggactgt gacacgactt
    29221 ttgtcacagt ccttttttta ctatgcagtt aattctttgc ccaattgggt tgccatcgat
    29281 agtttctttc tttacccaaa ataaattgga tcattgaaaa caccgcagca aaagcaacta
    29341 agacacaacc attgaaccaa aaagattctg caaatgtttg attgtaactg agtgtttcac
    29401 tgatcaagtt attattttga acaaaccaat atgttggtgt gaatgccgct attttgctaa
    29461 ccatttctgg caagtattct gctggtacaa acaccccacc gataaagcaa ctgcctgtga
    29521 taaaaatatc tttgatccca ccgctggttt ctgagttttt taccaatgaa gtgatacaag
    29581 ccgaaaagct aatgatcggc aataaaaaca agaaagtatt taaaatatga taccataccg
    29641 ttatatccca cgaggtgtgg atcactaaca gaaccattag aagtaagaag ccactgaaca
    29701 acagcagcga gtaactaatc aaactaaagg ttactttgcg attcaagttt cgtttggtaa
    29761 tgggcgcact ctggtttcgt ttactgatct ctttgcgatt aaatgctaga ttgatgacgc
    29821 tgaaaccgct aaaaatggat agaaacaacc cataagacaa gaaattaaag gttgtgcccg
    29881 tcaacagctt tttcatccgc tgactatatc ccgcacttag ctcaacttct ccagtaaccg
    29941 agagtgccat ttgtgtctgt tccatagctg catcgattgt tttatcttct tcttgaaaca
    30001 gaatcagtgt ttgcaaaaag gtgttcactt gctgtgtaac aactgtttta gaaaaggcat
    30061 ctgggcgact ctgtatcttg atcgtcggca tctttcctac ttcgacttgt tcggtaaagt
    30121 ctgcaggcag atgtaagata atgtttacac gattaaaata aagagcatca tcgatttctt
    30181 tttgtgaggt tccatccaat gtaacgactg tttccttgtc attcaaatat ttacgaaggc
    30241 catcgactac aggcgactct tcttcactaa tcaaggcgat tttcgcatct tgcagagagg
    30301 tattttcaac atcaatattt tgcgcaaaaa agaatgtggc aacaacagtg ataattacac
    30361 ctatcagtaa attcgattta tttgctttaa gcaatgtcca aatcgtttta taaagcgtca
    30421 taacgcaccc ctctttccat ccaagcattt cctaacgcaa aaaggaaaat aaatccgatc
    30481 aaccatagaa gattggtata aaaactagcc aaatcagtgt agaaataaag tttatacaaa
    30541 ctttcgctga tcaagttgac gagatttaat tgaccaacaa tcggaatatg aagatcaatc
    30601 caatacttca cttgttccga ccccatcata ccagcaaaaa agctcatcgc aatcgtcact
    30661 gaaatcaaga tgctatcctt ttgcaccaga ttcatttttg gtatcaaatt accaaccaat
    30721 gtacctaaca aaatcgcaca caacgacccc agagcggtaa caatcatcaa ccattgccaa
    30781 cgttcaccaa agtctacttg atagataaaa cgaaagacag ctaacataat ataaatctct
    30841 gtcaaaacaa tcgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta
    30901 ggaatcaagg acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg
    30961 cggacccccc acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa
    31021 cttttgaaac tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa
    31081 acagtcatga tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata
    31141 ccaatacgct gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct
    31201 tgacccacat ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct
    31261 gcatttagct tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta
    31321 tcattgactt cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc
    31381 gctttgatta caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca
    31441 aaggccgcca taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg
    31501 gatttatttc gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc
    31561 cctcctctag tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc
    31621 ttctttggtt gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg
    31681 caacaatact gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat
    31741 tccatcagaa gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg
    31801 tcaaacgatc cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca
    31861 cctgtccttg atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat
    31921 gtgttgtata gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt
    31981 tattccgact ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct
    32041 tgtgggcaat cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg
    32101 gacgaaacga aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt
    32161 tccttttctc tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac
    32221 ttagttcgtc aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat
    32281 aattttcagg agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca
    32341 aagaaagcat acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta
    32401 agacttctcc ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc
    32461 gtttgactaa tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt
    32521 gatctgacta taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg
    32581 acatttgtca caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc
    32641 tttctactgg ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt
    32701 atctatttat tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca
    32761 aattggattt tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg
    32821 tttctgccag cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt
    32881 ttgttaggtt ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt
    32941 gtcgtaattt tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat
    33001 cttgagaaaa agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg
    33061 gaacaaaata aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa
    33121 gaacggaacc gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg
    33181 attttacaaa ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta
    33241 gaaaatttaa gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac
    33301 ctccatgaga catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct
    33361 tcttctgtta cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc
    33421 ttcttaatgg tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc
    33481 gttgttttac attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc
    33541 accagcccac aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt
    33601 caagaacaaa agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc
    33661 attttaccaa gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt
    33721 gacgaaatcg ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg
    33781 tcattgtgcc aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac
    33841 ggacatccgc atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt
    33901 tcccaaagcc atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc
    33961 attttccatc ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc
    34021 tgtcaaatcc gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac
    34081 tcaaatgatg aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg
    34141 tgagcttgca atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc
    34201 cttgtacttg agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga
    34261 tttacgggat cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc
    34321 taacacaaaa aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct
    34381 attcaattgt cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg
    34441 ccaggattcg ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt
    34501 ttttctagtt ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg
    34561 aattcttttt ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc
    34621 gctaatttat cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta
    34681 gcatatcaca ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct
    34741 catctttaaa atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata
    34801 ccaatcatcg gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta
    34861 gaaacagcag gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct
    34921 ggttccactg tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca
    34981 ggtagctttt cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca
    35041 tttccgacca aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta
    35101 tcattatcca tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca
    35161 caagtgacat aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact
    35221 gccgtgtaga gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata
    35281 accatcttga attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt
    35341 ttattccatt cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca
    35401 tccactttat cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca
    35461 ttgaatacat tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa
    35521 acggtgtcgt tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc
    35581 atcattttat ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg
    35641 atatcttgtg cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt
    35701 tcaatttgat aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct
    35761 tttggcagac gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct
    35821 tgccgataaa tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg
    35881 agtacttgac caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc
    35941 atattcgtcg gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc
    36001 tcatcaacaa tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc
    36061 cgtccattca gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc
    36121 attcgtttag ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta
    36181 tattttgaag gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg
    36241 atcacagtag ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt
    36301 gcttcagcga tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata
    36361 cgccgaatat tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga
    36421 aggcgctgga tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga
    36481 ttagtctctt cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc
    36541 tgatcaggct ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc
    36601 aatgtctccg ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc
    36661 aagatagctg ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga
    36721 ttatctgcac gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct
    36781 tcgatgatat ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta
    36841 ccgaacccgt aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc
    36901 gtctcagcaa gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa
    36961 ggatcagcaa tgattttttc gatagcatct tctcccagca attctacaat tttttctgct
    37021 gttttcttcc cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca
    37081 gttgttggct tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga
    37141 tccactaatt ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg
    37201 gtgactacaa tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc
    37261 actttgtaaa aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa
    37321 ggcatttctt ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc
    37381 tgtttcagtt cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct
    37441 gcaaggctct tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg
    37501 caactcactt agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg
    37561 tcctacgaat aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc
    37621 gatacgtgtg attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc
    37681 taaatgatta cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga
    37741 ctgaccttct aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac
    37801 cggacgagca agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact
    37861 ggaataaatt gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc
    37921 gtaactttgt ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt
    37981 ccattgtgtc ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt
    38041 cttttatttt actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt
    38101 caattctaaa ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt
    38161 attcgataaa ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc
    38221 ttcgttttca cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa
    38281 gtgctcttct ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat
    38341 caaatgtagt ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt
    38401 aatatttaca tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga
    38461 aaaacctagt ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat
    38521 atttccagtg tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat
    38581 tcctctcata tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa
    38641 cgtatcaact cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac
    38701 ttttatcaga acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac
    38761 accctcaact tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct
    38821 tagttccttt taataattct atgtcaattg taatcttctt caaaggattc ttatataagt
    38881 ctgctttgat tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt
    38941 gattttcctc tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg
    39001 cttcagcaaa cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg
    39061 gataaaaagc ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt
    39121 ctccatcttc tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta
    39181 aaaccgcgtt gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa
    39241 atgcttcggc agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa
    39301 cagcattttg aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc
    39361 caacatctaa tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct
    39421 gatacatgcc agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg
    39481 ttttaaccgc atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat
    39541 tatttttgca tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc
    39601 ggaacagctg atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag
    39661 ttccccctca tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat
    39721 atgatacaat gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt
    39781 ttgcgctgaa aactacacat ctattcctct agcaattgct agaggagccg atcggatcga
    39841 attgtgtgac aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac
    39901 gttaagttat gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg
    39961 cgacttcgtc tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa
    40021 gaaattaggg acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga
    40081 agaggcctta gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc
    40141 ttttgacagt attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg
    40201 tgttgaccgt atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc
    40261 acggcttaaa gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg
    40321 tgtcactgca gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg
    40381 aaccaaaatc gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca
    40441 taatgcttat tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca
    40501 atattgaaat aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt
    40561 gctactgctg gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga
    40621 ttcgccactg tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta
    40681 tgtttccaaa aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg
    40741 ccggtcaaga cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc
    40801 gcaagtcgac gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta
    40861 atcagcgaaa tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg
    40921 attaaagaag cggtcgacat acctaaaaac aacagagtga tcagccaagg caaacgtcga
    40981 gacgctgcct ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa
    41041 taatcgctag cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct
    41101 aacaaatgat catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt
    41161 tgtgccacat cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga
    41221 tcagaaatca ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact
    41281 agctgacctt cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg
    41341 ttcttcagca cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca
    41401 gttgtcatga tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg
    41461 gcatcgtctg ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta
    41521 agcaagtcaa cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga
    41581 cgcatttcat ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata
    41641 tccgctaatt ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga
    41701 gattggtaga attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc
    41761 tgaatattat tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga
    41821 ttttcatcca aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca
    41881 tgtctgcggc taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac
    41941 gcagatcata acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca
    42001 tatcatcacc gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc
    42061 ttcctgtatg cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt
    42121 actcagtctg tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat
    42181 ctcgggcgat cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga
    42241 ctaaggcctg atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt
    42301 gcgcaaatcc atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca
    42361 ccacatggat tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag
    42421 ccattgtccc atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta
    42481 ctaccagaac gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg
    42541 tttcatgttc tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa
    42601 acaagacatt ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg
    42661 ctaacagacc ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat
    42721 ctttctcata atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca
    42781 cgatgccaaa aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga
    42841 taataaattg agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa
    42901 tcttctgtat tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa
    42961 cccaaagtac gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg
    43021 atactaggat ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc
    43081 gtagaaatcg tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca
    43141 tcggctacca tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc
    43201 tggtccggtg tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt
    43261 tgttcccggt tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc
    43321 gtgtaaaacc ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga
    43381 ctgtagtgat ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct
    43441 ggctcatgat catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc
    43501 acttctatag tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc
    43561 tctttcgctt tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc
    43621 atgggagaat aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc
    43681 caactgatag gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac
    43741 gccttgatat ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt
    43801 ccgaatctgg atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc
    43861 gatcaccaga tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc
    43921 ttggagaact ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc
    43981 tacaaactga cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc
    44041 cattggaata tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc
    44101 agtgacaaat tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg
    44161 cagtttcact ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc
    44221 tcgttccata gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc
    44281 aaagtttttt cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag
    44341 acaaaaagga gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac
    44401 aaagcaagag tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt
    44461 acagactaat atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct
    44521 acggatccgt ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg
    44581 gcgtctcgaa acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa
    44641 acgtctgcct cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc
    44701 ttcattaatc caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg
    44761 acttttagct attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga
    44821 ggtaagggga tgcatctgtg ggaaaaattg cttttataaa ttttttaaaa cgtttttctt
    44881 taccttatcg tcctgctaag aacaagatcc aacgtatgct tgaagctaaa aattagaaac
    44941 ctttcatttt cacaaggcac ttgagttcat ttttttactt ttttctgtta agttatagga
    45001 tgtgaaagaa tctagtcact agttgtatag aatctatttt gggggagatt catatgatcg
    45061 aaatctattt atttgttaat cctttagggg aatactgttt cgaaatggaa caacagttat
    45121 tacaatttat cgaagaagag tacgggacac catcaaaaga aaagatgcaa tttcgctttc
    45181 ttcctttggt caatttgcag acaattgggg atgtcatgca acaaaaagga atttctcaga
    45241 acaatcttga ggaacgtaat cacttattct caatgactta ttctgcagca ttggactgca
    45301 aagcggcgca gtttcaagga aagaaaaaag gccgccgttt tttgatcaaa ttgcaagaag
    45361 cagtcggctg taatgacata ccatattctc aagaacttgc tgaatccata tttgaagaaa
    45421 tcggcggcga tttgaatatg tttaaagaag atcgtcagtc tgacttcgtt aaagaagtat
    45481 tcttttctga tcaaaaaatt gctcgggaaa tgggcatcac aaaacaccct tctgcagtcg
    45541 tttacaatta tgcctgcgac cgtgattttg gtgttttagt agaagtaaaa ggtacaagta
    45601 gcctttggag aatcacagag ctgtgtaaaa caacagataa ctcatatcaa gtttttaatg
    45661 gtgaacattt acatacaaat gattctcagt tccattcaag aactagccat ttacacttgc
    45721 tttctaatta aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt
    45781 ctattcgaga aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca
    45841 tttagacgtt gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca
    45901 actcctgctt ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc
    45961 aagtagttct ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca
    46021 gcagaaaagc cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt
    46081 gaccattcga atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc
    46141 aatttaccat aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc
    46201 gtatgcatga aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac
    46261 ccatccaagt aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat
    46321 aaatactccg ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag
    46381 tagtcgccgt acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc
    46441 atttcgtgga caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat
    46501 ggatttgtgt catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca
    46561 acatcgatcc aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt
    46621 ggtttcagtg cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct
    46681 tcccctaaga ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt
    46741 ttccgtaatt ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa
    46801 gtatcgtata cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg
    46861 taattacgga ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca
    46921 aatgtatttc taaattgttc gtaaacactg tacaatccct taaatgctgc ttcgcgcacc
    46981 cttcgatccg tgctttctag caactgacca tacacgccat gagataattg gactatttca
    47041 ccattttctc cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta
    47101 tcacttgaag catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat
    47161 aagacatggg ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt
    47221 ggttcttctt tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca
    47281 aaccaggaaa cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct
    47341 tggtaatctg tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact
    47401 tcagtttggc gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct
    47461 tgatcaagtg ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat
    47521 ttctcatcaa actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca
    47581 ggcaattctt ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc
    47641 ttagctctat ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt
    47701 ttctactcat tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat
    47761 agaaaaccga ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat
    47821 gcattcgtta aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc
    47881 ccttttatgt tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc
    47941 caaggcttct tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa
    48001 ccgacttttt tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca
    48061 ttgttcttgt atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt
    48121 cttcccatct tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact
    48181 agtgaatatt tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat
    48241 atgccctgat acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc
    48301 agctttccag caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa
    48361 tgagaattgc gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt
    48421 attcaaataa ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat
    48481 ttctattgct agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc
    48541 ttccaaaaat actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg
    48601 taagtgctct tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc
    48661 aaaatgagca cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca
    48721 ataatatttc tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat
    48781 tgcaagcagc atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa
    48841 aaagagggcg tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt
    48901 tcccaatatt tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt
    48961 atacgaaaaa aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta
    49021 tacaaaataa aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc
    49081 attcaatgtt tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg
    49141 attctctaca tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg
    49201 ctcttcgaat aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg
    49261 gatcacttgt tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc
    49321 gcttccagat aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact
    49381 cacatcttct gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt
    49441 tttactgata aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc
    49501 gcttcctcgg aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc
    49561 gatttctttg tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc
    49621 atgaccaatt agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca
    49681 cctcactttg ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa
    49741 gaaaaaaaat ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa
    49801 gctatcttat ttaactataa catttttggc agacgaatac tatatagccg acttttcagc
    49861 taattttccc tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta
    49921 atcaagaata ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt
    49981 t
//
LOCUS       DAZKOU010000010        47903 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000010 DAZKOU010000000
VERSION     DAZKOU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.010"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..502
                     /locus_tag="ACG827_000524"
     CDS             131..502
                     /locus_tag="ACG827_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0482690.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(776..1468)
                     /locus_tag="ACG827_000525"
     CDS             complement(776..1468)
                     /locus_tag="ACG827_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0482691.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1471..2256)
                     /locus_tag="ACG827_000526"
     CDS             complement(1471..2256)
                     /locus_tag="ACG827_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0482692.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2493..3893
                     /gene="lacG"
                     /locus_tag="ACG827_000527"
     CDS             2493..3893
                     /gene="lacG"
                     /locus_tag="ACG827_000527"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0482693.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3918..4235
                     /locus_tag="ACG827_000528"
     CDS             3918..4235
                     /locus_tag="ACG827_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0482694.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4248..5921
                     /locus_tag="ACG827_000529"
     CDS             4248..5921
                     /locus_tag="ACG827_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0482695.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5935..7566
                     /locus_tag="ACG827_000530"
     CDS             5935..7566
                     /locus_tag="ACG827_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0482696.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8057..9622)
                     /gene="guaA"
                     /locus_tag="ACG827_000531"
     CDS             complement(8057..9622)
                     /gene="guaA"
                     /locus_tag="ACG827_000531"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0482697.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9681..10604)
                     /gene="coaA"
                     /locus_tag="ACG827_000532"
     CDS             complement(9681..10604)
                     /gene="coaA"
                     /locus_tag="ACG827_000532"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0482698.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10800..11405
                     /locus_tag="ACG827_000533"
     CDS             10800..11405
                     /locus_tag="ACG827_000533"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0482699.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11713..12729
                     /locus_tag="ACG827_000534"
     CDS             11713..12729
                     /locus_tag="ACG827_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0482700.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12870..14171
                     /locus_tag="ACG827_000535"
     CDS             12870..14171
                     /locus_tag="ACG827_000535"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0482701.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14217..14879
                     /gene="deoC"
                     /locus_tag="ACG827_000536"
     CDS             14217..14879
                     /gene="deoC"
                     /locus_tag="ACG827_000536"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0482702.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14900..15298
                     /locus_tag="ACG827_000537"
     CDS             14900..15298
                     /locus_tag="ACG827_000537"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0482703.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15364..16446
                     /locus_tag="ACG827_000538"
     CDS             15364..16446
                     /locus_tag="ACG827_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0482704.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16658..18220
                     /locus_tag="ACG827_000539"
     CDS             16658..18220
                     /locus_tag="ACG827_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482705.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18213..19346
                     /locus_tag="ACG827_000540"
     CDS             18213..19346
                     /locus_tag="ACG827_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0482706.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19346..20302
                     /locus_tag="ACG827_000541"
     CDS             19346..20302
                     /locus_tag="ACG827_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0482707.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20401..21567
                     /gene="deoB"
                     /locus_tag="ACG827_000542"
     CDS             20401..21567
                     /gene="deoB"
                     /locus_tag="ACG827_000542"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0482708.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21591..22409
                     /locus_tag="ACG827_000543"
     CDS             21591..22409
                     /locus_tag="ACG827_000543"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0482709.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22426..23133
                     /gene="deoD"
                     /locus_tag="ACG827_000544"
     CDS             22426..23133
                     /gene="deoD"
                     /locus_tag="ACG827_000544"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0482710.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23431..24117)
                     /gene="gpmA"
                     /locus_tag="ACG827_000545"
     CDS             complement(23431..24117)
                     /gene="gpmA"
                     /locus_tag="ACG827_000545"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0482711.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24289..24870)
                     /locus_tag="ACG827_000546"
     CDS             complement(24289..24870)
                     /locus_tag="ACG827_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0482712.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25003..25686)
                     /gene="rpiA"
                     /locus_tag="ACG827_000547"
     CDS             complement(25003..25686)
                     /gene="rpiA"
                     /locus_tag="ACG827_000547"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0482713.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26005..26418
                     /gene="rpsL"
                     /locus_tag="ACG827_000548"
     CDS             26005..26418
                     /gene="rpsL"
                     /locus_tag="ACG827_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0482714.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26502..26972
                     /gene="rpsG"
                     /locus_tag="ACG827_000549"
     CDS             26502..26972
                     /gene="rpsG"
                     /locus_tag="ACG827_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0482715.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27066..29153
                     /gene="fusA"
                     /locus_tag="ACG827_000550"
     CDS             27066..29153
                     /gene="fusA"
                     /locus_tag="ACG827_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0482716.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29325..30512
                     /gene="tuf"
                     /locus_tag="ACG827_000551"
     CDS             29325..30512
                     /gene="tuf"
                     /locus_tag="ACG827_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0482717.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30919..31227
                     /gene="rpsJ"
                     /locus_tag="ACG827_000552"
     CDS             30919..31227
                     /gene="rpsJ"
                     /locus_tag="ACG827_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0482718.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31258..31887
                     /gene="rplC"
                     /locus_tag="ACG827_000553"
     CDS             31258..31887
                     /gene="rplC"
                     /locus_tag="ACG827_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0482719.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31915..32538
                     /gene="rplD"
                     /locus_tag="ACG827_000554"
     CDS             31915..32538
                     /gene="rplD"
                     /locus_tag="ACG827_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0482720.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32538..32828
                     /gene="rplW"
                     /locus_tag="ACG827_000555"
     CDS             32538..32828
                     /gene="rplW"
                     /locus_tag="ACG827_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0482721.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32867..33700
                     /gene="rplB"
                     /locus_tag="ACG827_000556"
     CDS             32867..33700
                     /gene="rplB"
                     /locus_tag="ACG827_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0482722.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33743..34021
                     /gene="rpsS"
                     /locus_tag="ACG827_000557"
     CDS             33743..34021
                     /gene="rpsS"
                     /locus_tag="ACG827_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0482723.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34044..34391
                     /gene="rplV"
                     /locus_tag="ACG827_000558"
     CDS             34044..34391
                     /gene="rplV"
                     /locus_tag="ACG827_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0482724.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34405..35061
                     /gene="rpsC"
                     /locus_tag="ACG827_000559"
     CDS             34405..35061
                     /gene="rpsC"
                     /locus_tag="ACG827_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0482725.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35064..35498
                     /gene="rplP"
                     /locus_tag="ACG827_000560"
     CDS             35064..35498
                     /gene="rplP"
                     /locus_tag="ACG827_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0482726.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35488..35676
                     /gene="rpmC"
                     /locus_tag="ACG827_000561"
     CDS             35488..35676
                     /gene="rpmC"
                     /locus_tag="ACG827_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0482727.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35701..35967
                     /gene="rpsQ"
                     /locus_tag="ACG827_000562"
     CDS             35701..35967
                     /gene="rpsQ"
                     /locus_tag="ACG827_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0482728.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36025..36393
                     /gene="rplN"
                     /locus_tag="ACG827_000563"
     CDS             36025..36393
                     /gene="rplN"
                     /locus_tag="ACG827_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0482729.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36430..36738
                     /gene="rplX"
                     /locus_tag="ACG827_000564"
     CDS             36430..36738
                     /gene="rplX"
                     /locus_tag="ACG827_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0482730.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36766..37305
                     /gene="rplE"
                     /locus_tag="ACG827_000565"
     CDS             36766..37305
                     /gene="rplE"
                     /locus_tag="ACG827_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0482731.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37324..37509
                     /locus_tag="ACG827_000566"
     CDS             37324..37509
                     /locus_tag="ACG827_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0482732.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37545..37943
                     /gene="rpsH"
                     /locus_tag="ACG827_000567"
     CDS             37545..37943
                     /gene="rpsH"
                     /locus_tag="ACG827_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0482733.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37975..38511
                     /gene="rplF"
                     /locus_tag="ACG827_000568"
     CDS             37975..38511
                     /gene="rplF"
                     /locus_tag="ACG827_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0482734.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38672..39028
                     /gene="rplR"
                     /locus_tag="ACG827_000569"
     CDS             38672..39028
                     /gene="rplR"
                     /locus_tag="ACG827_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0482735.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39049..39549
                     /gene="rpsE"
                     /locus_tag="ACG827_000570"
     CDS             39049..39549
                     /gene="rpsE"
                     /locus_tag="ACG827_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0482736.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39564..39743
                     /gene="rpmD"
                     /locus_tag="ACG827_000571"
     CDS             39564..39743
                     /gene="rpmD"
                     /locus_tag="ACG827_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0482737.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39787..40227
                     /gene="rplO"
                     /locus_tag="ACG827_000572"
     CDS             39787..40227
                     /gene="rplO"
                     /locus_tag="ACG827_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0482738.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40227..41522
                     /gene="secY"
                     /locus_tag="ACG827_000573"
     CDS             40227..41522
                     /gene="secY"
                     /locus_tag="ACG827_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0482739.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41584..42231
                     /locus_tag="ACG827_000574"
     CDS             41584..42231
                     /locus_tag="ACG827_000574"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0482740.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42422..42640
                     /gene="infA"
                     /locus_tag="ACG827_000575"
     CDS             42422..42640
                     /gene="infA"
                     /locus_tag="ACG827_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0482741.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42673..42789
                     /gene="rpmJ"
                     /locus_tag="ACG827_000576"
     CDS             42673..42789
                     /gene="rpmJ"
                     /locus_tag="ACG827_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0482742.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42807..43172
                     /gene="rpsM"
                     /locus_tag="ACG827_000577"
     CDS             42807..43172
                     /gene="rpsM"
                     /locus_tag="ACG827_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0482743.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43200..43589
                     /gene="rpsK"
                     /locus_tag="ACG827_000578"
     CDS             43200..43589
                     /gene="rpsK"
                     /locus_tag="ACG827_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0482744.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43669..44607
                     /locus_tag="ACG827_000579"
     CDS             43669..44607
                     /locus_tag="ACG827_000579"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0482745.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44635..45015
                     /gene="rplQ"
                     /locus_tag="ACG827_000580"
     CDS             44635..45015
                     /gene="rplQ"
                     /locus_tag="ACG827_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0482746.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45287..46126
                     /locus_tag="ACG827_000581"
     CDS             45287..46126
                     /locus_tag="ACG827_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0482747.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46102..46971
                     /locus_tag="ACG827_000582"
     CDS             46102..46971
                     /locus_tag="ACG827_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0482748.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46961..47758
                     /locus_tag="ACG827_000583"
     CDS             46961..47758
                     /locus_tag="ACG827_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0482749.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        15143 a         8764 c        10509 g        13487 t
ORIGIN      
        1 gagaacgggt tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg
       61 ggaaaacgaa gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc
      121 cgataggata atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca
      181 gtcattaaat gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg
      241 gattagtgac tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt
      301 acgcatgata aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga
      361 tacttaccta gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt
      421 tatttcctct ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga
      481 ggaactagtg gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag
      541 tactcctgat gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt
      601 taattataga tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca
      661 ctgctatatt tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt
      721 ttataaaagg aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag
      781 aattaacgga tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc
      841 catctttatt tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg
      901 caataattgt tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga
      961 taacaagaca atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg
     1021 gttgcggttt cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc
     1081 gggctaaatc gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa
     1141 tcgttaatgg aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca
     1201 actgaagctc gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg
     1261 acaatacggc gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg
     1321 tattcgtccg accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat
     1381 gatcatgtat catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc
     1441 cgttgaaatc aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga
     1501 atcagcaatt tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg
     1561 gcgatccgta atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg
     1621 attgaatttt ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg
     1681 ctgtgcttct ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga
     1741 actgataaat gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac
     1801 aaatgcgccg gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc
     1861 ttgagcaaga atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag
     1921 taaatgcgtc gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc
     1981 gtgaatcaag gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc
     2041 tttttcagtg tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg
     2101 gacacgcagc agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc
     2161 agaaacatct aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat
     2221 ttctacgata gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat
     2281 aatctatcat ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt
     2341 ggtttttatg tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt
     2401 gtttgttttt gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg
     2461 aaaactgatt atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt
     2521 ttggcggagc cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg
     2581 ctgttgcttg ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg
     2641 atttttatca tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc
     2701 taagattatc cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag
     2761 aaggaatcgc tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt
     2821 ttgtgacgtt gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga
     2881 atcgagaaac aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag
     2941 aagtcaaggt gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt
     3001 taggtgtttt cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata
     3061 atatgatggt tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg
     3121 aaattggtgt agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata
     3181 agcatgctgc ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt
     3241 taggctatta ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg
     3301 ccagctatca ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg
     3361 actacttagg gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg
     3421 caatccatca taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg
     3481 gagaacgtat ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag
     3541 aaggtctata tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct
     3601 atatcactga gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc
     3661 agccgagaat cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg
     3721 gggtaaatgt gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg
     3781 ggtataacaa aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa
     3841 aagaatcagc ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga
     3901 taatggagga agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg
     3961 catattcagg cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa
     4021 actttgaaca tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata
     4081 attcgcaaac aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca
     4141 tttttatcca tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag
     4201 actttattgt actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca
     4261 ttaaacaaat cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag
     4321 gagccgttcg tgacggattt ttaacagcga tgccagctat tttattttca agcattttca
     4381 ttttgattgc tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat
     4441 ggctatggaa agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca
     4501 ctgctcgctg cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca
     4561 ccacctctgt gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg
     4621 aaggtggatt ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg
     4681 catttttaac agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc
     4741 ctaaagaagt tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt
     4801 cagtatttgc ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg
     4861 cagaagcaat cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa
     4921 tcgctattat ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga
     4981 tcgtggagcc ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt
     5041 tcaattccgg tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa
     5101 ctatgggagg aactggggca acattggttg ttccttttat cttcctactc tttgctaagt
     5161 ctaagcagtt gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg
     5221 aaccattgct atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt
     5281 taacacctgt tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca
     5341 gctttatgta cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg
     5401 gcatgggtat cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct
     5461 atctaccatt ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag
     5521 aggtgatcgc aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag
     5581 cgagtcaagt tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg
     5641 cagggacaag tgggatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac
     5701 cgatcagtgc aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata
     5761 tggtggtctt agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc
     5821 gcttaggaat caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc
     5881 ctgaaaaagc tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa
     5941 atgggaattt ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct
     6001 gtttcaaaaa ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc
     6061 ctgtcagata tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc
     6121 ttagagattt taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct
     6181 tataatagta atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac
     6241 cttgcaaaac gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat
     6301 ttttgggttg atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa
     6361 gaaatgaata cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg
     6421 gacgtttatc ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct
     6481 tatggcaagt cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag
     6541 tctggtatcc aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta
     6601 gctgatggtg gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt
     6661 gtgtcatttg atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa
     6721 cttcaaacca atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa
     6781 acagcttatg ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc
     6841 gaagcagctg cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat
     6901 cttgccgatc ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa
     6961 cctctttggt acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt
     7021 gttcaatcca ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac
     7081 gccttgccaa gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat
     7141 gcaagtttcg aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa
     7201 caaggaaccg ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa
     7261 ctaacttttt ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata
     7321 agcaaaggaa cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat
     7381 cagttatata tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt
     7441 gaatggacaa aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg
     7501 gtttatagca aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa
     7561 caatagactg cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc
     7621 gtagaaggca ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc
     7681 cagtcatttt taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta
     7741 aagagagttc aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa
     7801 cataaagttt gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac
     7861 ttagatggat agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg
     7921 ataagaatga aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt
     7981 ttaatgaaaa tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc
     8041 atattatgtt ggctctttat tcccactcca ctgttgctgg tggtttagaa gtgatgtcat
     8101 acacgatacg gttgacgtgg gctacttcgt ttacgatacg tacagagatt ttttgcaaga
     8161 cttcccatgg gatacgggca aagtcagcgg tcattccgtc gattgaagtt acagcacgga
     8221 taccgactgt gtagtcgtaa gtacgtccgt cacccataac accgacagag cggatccctg
     8281 gaagaacagt gaaatattgc cagatatcac ggtcaagtcc agcagctgcg atttcttcac
     8341 gcaagatagc atcagattcg cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa
     8401 cacgaatacc aagaccagga ccagggaatg gctgacgcca aacgattgaa tcaggcatgc
     8461 ctaactgagt ccctaattca cggacttcgt ctttgaataa tgtgtttaat ggttcgatca
     8521 attcaaattg catatcttct ggcagtccac ctacattatg gtgagatttg atcgtctgtg
     8581 ctgtttctgt accagattcg ataacgtcag tatacaacgt tccttgtgct aggaaagaga
     8641 cgccttcttc accagcaagt tttgttgctt cgtcatcaaa aacatatacg aattcattcc
     8701 caatgatttt acgtttttgt tctgggtcag atacaccttt caacttgtct aagaagcgat
     8761 ctcttgcatt gactttgatg atgttcaagc cgaattttcc gcctagactt tccattactt
     8821 gttccgcttc gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc aattggtcac
     8881 cgatcgcttt ttgtaaaaga accccaacga cactagaatc aacgccacct gaaagaccaa
     8941 gcaagacttt tttgtcacca acttgttcac ggattttttc tacttgcata tcgataaagt
     9001 tatccattgt ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt
     9061 cgattccgta ttcggaatga cgtacttctg catggaattg gataccataa aaattacgtg
     9121 ttgtgtcttg gatagaagca atcggacaat ctttgcttgt tccgactgtt tcaaagcctt
     9181 caggaacttt agttaccaag tcaccatggc tcatccatac tgtttgttgt tttggtgttt
     9241 ctttaaacaa gacagcttga tcgcttgtta cttctaacaa ggctttccca tattcgcggt
     9301 ttttagcagg ctctacggaa ccgccaagtt tgtacgtgat caattgcata ccatagcaaa
     9361 tccctaatac aggaatacct aaattgaaga tctcaggatc gatcccaaat gcattctcat
     9421 catatacact gtttggtccg ccagaaagaa tgattccctt tggattcatc gcttttactt
     9481 cttctgcagt aatgcggtgg ctcaataatt cggaaaagac tccaagatca cgaatacgtc
     9541 ttgtgatcaa ttggttatac tgactaccgt agtctaatac gataatcttt tcaacgttcg
     9601 tcatatcggc aacgtttgtc aaatttatta cccctatccc tttaaatttt atctcaaaat
     9661 agtcaaacta ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt
     9721 tcagttttat ggagaattat atcggctctt cctcgtgtag gcaagatata ttcctgtaaa
     9781 tttttcaaat tcacattttt ccatacatct tttgccattt taaaggcttc ttcacgatct
     9841 ccgatagcaa aacgataata ataattctct ggattttgga aagccgtatc cagtaacgca
     9901 ccaaaacgat ccaaatacca tttttcgatc agcacgggat cggcatcgac atagacagaa
     9961 aaatcaaaaa agtcgctgac gtagatttgc tgattcgcag gtaattgaag cgtattgatc
    10021 ccctcaacga tcaagatatc aggctgctgg atcaattcgt acttgtctgg aatgatatca
    10081 tacacatcat gtgagtaaac aggtactttt atctcttcct gaccagattt tacacggttc
    10141 aagaaatcga tcagcatctc catatcataa ctttcaggaa atcctttacg gtccatgatt
    10201 ccacgctctt ttaatatacg atttggataa agaaatccgt cagtcgtgat cagctgaacg
    10261 tttcttcgtt tgaaggtacg agacaaaatc gtctgcagca gtctggctgt tgtacttttt
    10321 ccaacggcta cacttccggc gatcccgata ataaaaggcg gtacttctac gtatttatga
    10381 agaaataaac ctttactcaa gaacaaagat tcaaattctt tcatataaag ataaatcaga
    10441 tgagcaagag gtacatagat atctcgaaca tcctgcatgg aaatgcggtc attcaaactt
    10501 ttgatattat ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc
    10561 cattcttctc ttgccacacg gtagtagttc attggatcat tcatattagc ctcctgcgtt
    10621 ctcccatcaa tggaactatt ttaacacaaa ataaacgcaa accccaccac ttaagagaga
    10681 ttgccgatct ttttgtataa tctttataat agaaagaaac ggagtcttat tcttgacttg
    10741 cgcacggata cataaaagga tagactgttg tcatgaaaga aatgagaagg atggaagaca
    10801 tgacaaatca ttactattct gaacgcccag atacggcgca cgattttgaa caatgggctt
    10861 ttgagttaaa aggcagaacc tttcaatttg taacagattc tggtgtattt tcacgtgaaa
    10921 cagtggatta tggttcacgt gtcctgattg atgctttcga ttgggaagag ttacctgatg
    10981 aaggaaagat cttagatgtg ggatgcgggt atggaccgat tgggttagct ttggcatttg
    11041 cgacacagcg gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag
    11101 gaaacgcaaa gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat atctatgaag
    11161 cagttcatga agaaacctat gcagctatcg taagcaatcc gccgatccgt gctggaaaaa
    11221 aagttgtaca tgaaattttg acaggcgcat atcctcggtt gaagaagggc ggtacactga
    11281 caattgtgat ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat
    11341 ttggtaatgc agaaatcgtc acaaaagata aaggatatta tatcatcaaa agtgtgaaag
    11401 aataactagc tgaaaaatag tggagcgacg aaagagtgga actgggcgtg catgtacggg
    11461 tacgtatcag ttcttttttt gttgagaagt ttttaaaaac aaagaactat tttttcttca
    11521 aaaagaaata gtaggaacag agtcgttgtt ttttacgata aaataaataa gatacagaac
    11581 ttgtatagaa ggctgccaag tcggtagata aaggaggttt ttatcaaaaa acatactttc
    11641 cttttttctc tattcttttc ttgggggttt tggtacactg atagggaatt tagatagaag
    11701 ggagccaata ggatgaagct gaccatcaga gatatcgcag aaatggcagg cgtatcagta
    11761 acaactgtct cacagatttt gaataacaaa ggaagcagat ttagtgataa gacaagaaaa
    11821 aaagtgttgt cgatcgtgaa tgaacatcat tataagcccg attatttcgc ttcaaatgtg
    11881 atcaatcgac attcaaagac catcggaatg atcgtccctg atgtcactga tttcttcttc
    11941 tccaaactga tcgaaggtgt agagagctat ttgaatcctt tgggctatgt gatcatgtta
    12001 tgcaattctc gtcatagcca agaaaatgag atcaagtatt tacaagagct ttcccaccgc
    12061 tcagtggacg gaatcttgct ggccacacca aatattttac ctgagaaata tgcattagac
    12121 agcggttttt atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat
    12181 aacggacggc ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc gctgtttatt
    12241 gaaaatggcc atacgaagat cggcatgctg aaggaaacca ctggatacta tcaattagaa
    12301 gaacgtttca acggatatcg tcatgcgctg aaagatcatg gcctgccttt taatgggaaa
    12361 tttgttgaac atggagagtt gactgtccaa ggtggatata ctgcatcaaa aaaattactc
    12421 aaacataaag atatcacggc tattttttgc gggaatgatg ccatggcgat cggatgctat
    12481 caggcgatcg atgaattggg caaaaaaatc ccagaagata tctcagtcat aggattcgat
    12541 ggattaaagc tgtcagaata catgattccg caattgacga cagtgcagca gccaagtttt
    12601 gatattggat tttatgctgc acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa
    12661 gttccgaaca aagtgttcga aacaaaatta attattcgtg agagcgtaaa agtattgaca
    12721 aagggctaac tcggcggtat attgcttatg taagcattta caaaggctag ttagtgcttt
    12781 ttttattttt agtaaaccag tttactaaag tggtttacct ttcctaaaaa taatgtataa
    12841 taccgaatga agacaaggag gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga
    12901 gggatggaaa cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg
    12961 gaaagattcc agattatcaa gtcagtgcat tattgatggc aattttttat caagacatga
    13021 caaatgaaga aatcacgaac ttaacattgg cgattgctaa ttctggtgat gtcatcgatt
    13081 tgagttctct tgaaggaata aaggtagata agcattcgac aggtggcgtt ggtgacacga
    13141 ccacattgat tttagccccc ctagtagcta gtgtcggagt gacagtggct aaaatgtctg
    13201 gacgcggtct aggttacaca ggtggtacac tagacaagct agaagcaatt cccggatttc
    13261 agatcgagct ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa
    13321 ttggtcaatc aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca
    13381 cagcgaccgt tgattctctt ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg
    13441 caggagcaga tgcaatcgtt ttggatgtga ccactggaga cggtgctttc atgaagaaca
    13501 tcgaagatgc aagacgtctg gcaaagacca tgacgagcat tgggaaactg gctaaccgag
    13561 aaacagtggc ggttatctca gatatgtcag aacctcttgg cgaagctatc ggaaatagtc
    13621 tagaagttgt cgaggcaatc gaaacattac aaggaaatgg tccagaagat ctagtagaaa
    13681 tgtgctacgc cttaggaagc caaatggttg ttcttgctgg caaagctaag acaaccgatg
    13741 aagcaagaac gttgcttcaa gaagctctgg aatcaggtaa agcgttagca aaattcaaag
    13801 aaatgatcca aaatcaaggg ggagacccag cgatcgtgga acatccagaa cgaattttga
    13861 ctgcacgcta cacaatggag cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg
    13921 caaatgaatt aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag
    13981 atattgatca cgctgttgga ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag
    14041 gtgagtcact attgacgatc tatagtaacg acgaagaaat cacttctgtc attgaattat
    14101 tatataaaaa tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt catgacatca
    14161 tcacagaata aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga aaagaaatgg
    14221 aactaaaccg aatgatcgac catactattt tgaaagcaga cgcatctaaa gaagctgtga
    14281 tgcaaattat cgaagaagca aaaaaatacc atttttattc tgtctgcatc aatccagcat
    14341 gggttgcgct agcgaaagaa caattacaag gtgagccagt agccgtatgt acagtaatcg
    14401 ggtttcctct aggagcaaac acacccgaga caaaagcatt tgaaacaaca gatgccatca
    14461 ataacggtgc agatgaagtc gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg
    14521 atcaacaagt ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg
    14581 tcaaagttat tattgaaaca tcgttattag atagagaaga aatcatccga gcttgcgaaa
    14641 tagcaaaagc tgcaggcgct gattttgtca aaacatcaac tggtttctca acaggcggag
    14701 caaaggtaga ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag
    14761 cttccggcgg tatccataac gaagaagaag caatggcaat gatcgaagct ggtgctacaa
    14821 gaatcggaac aagcgcagga gtagcaatcg tatctggttc aactggtgaa ggctattaat
    14881 tttttcaaag gagaagctca tggcagaact caaacaagaa tggatcaaca cagctattga
    14941 agcgttagat aaagcctatg ttccttattc ccattttccg gtaggcgctt gtctcgtaac
    15001 ggaatcaggc aagatttatc aaggcatcaa cattgagaat gcatcatttg gattaacgaa
    15061 ttgtgccgag cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa catttacgca
    15121 tctagtcgta gcggggcaca cccccgatcc tatctctcct tgtggtgcat gtcgacaagt
    15181 aatggcagaa ttctgttccc ctgatatgcc ggttacgcta gtaggagaca atggtgtgac
    15241 gaaggcaacg accgttcgtg cactgctgcc ttatgcattt actgaaaaag atctataact
    15301 catgttcatt cagtcagctg actgttgaaa aataaaacaa ttattttagg aggctttaca
    15361 gaaatgaaaa aagcaaaatt atttggttta ggtgctgtcg cactagcagc agggttattt
    15421 ttaggtgcat gcggcaacaa cggctctact gattcatccg gtggcaaaag ctcatcagac
    15481 acaacaactg cagcattgat cacagacaca ggtggtgtag acgaccgttc attcaaccag
    15541 tcagcttggg aaggactaga aaaatggggg aaagatcaag gactttcacg aggaaatgat
    15601 ggcttccaat atttccaatc ttcaaatgaa tcagattaca tcccaaatat tgaccaagca
    15661 ttgaatgctg gattcaaaac gatttttggt attggttata aactaaaacc agcgatccaa
    15721 gaacaagcaa caaacaatac aggtacgaac tttgttatta tcgatgatgt catcgatgga
    15781 ttagataacg ttgtatctgc aactttcaaa gacaacgaag cttcttatct tgcaggtgtt
    15841 gcagcagctt atacgactga aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa
    15901 gtaatcgatc gttttgatgc tggattcaaa gccggtgttg atgcaggtgc gaaagaatta
    15961 ggaaaagaaa tcaaagtatt gaatcaatat gctggtgact tctctgctcc agataaagga
    16021 cgttcaattg ctcaaggtat gtatgcacaa aacgcagata tcatcttcca tgcttcaggc
    16081 ggtacaggta acggtgtatt ccaagaagca aaatcattga acgaatcagg cgacaaaaaa
    16141 gtttgggtta tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac attaaacggc
    16201 gaaaagaaaa acttcacatt gacatctaca ctgaaagcag ttggaacagt agtagaagat
    16261 ttggcacaaa aatcagcgga cggtaaattc cctggcggcg aacacactgt ttatggattg
    16321 aaagaagacg gtgttggtct aacagaagga caactttctg acgaagcgaa aaaagcagtt
    16381 gatgaagcaa aagaaaaaat catttcaggt gacgtaaaag ttccagaaac tcctgaagaa
    16441 aactaaaacc atttctatag ccttgttaga cacatgtttg acaaggctat ggtttgatta
    16501 gaacaagaat tgttcattta tccaagctgc cttgtgacag tttggataag ggaacaactc
    16561 cctgaagtaa tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg
    16621 ttgccctaga tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa atattgtcat
    16681 agaaatgcag aatattacca aagaatttgg aacattcaag gcaaatgaca acatcaattt
    16741 acaagtaaag gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac
    16801 attgatgaac gtgctttccg gattattaga gccgacatca gggaaaattt tgatgcgtgg
    16861 gaaagaagta aagatcacaa gtccgaccaa agccaaccaa ttagggattg ggatggtcca
    16921 tcagcacttt atgcttgttg atgcctttac tgtaacagaa aacatcgtgt tgggaagcga
    16981 gcctagtcgc gcagggatgc ttgaccataa aaaagcacga aaagagatcc aaaaagtttc
    17041 tgaacagtat ggattgtcag tcaacccaga tgcttacgtt cgtgatattt cagttggaat
    17101 ggaacaacgg gtagaaattt taaaaacact ttaccgagga gcagatgtac tgatttttga
    17161 tgagccgaca gctgtattga cccctcaaga aattgatgaa ttaatcgtga tcatgaagga
    17221 attagtcaaa gaaggcaagt cgatcatttt gattacgcat aagttagatg aaatcaaagc
    17281 agtagctgac cgctgtacag ttatccgccg tggaaaagga atcggtacag tcaacgttaa
    17341 agacgtaacc tcacaacaat tagccgatat gatggttgga agaacggttt cattcaaaac
    17401 gatgaaaaaa gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa
    17461 agaaaatcgt ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt
    17521 acttggtatc gctggaatcg atggaaacgg gcagtcggag ttgatccaag ctttgactgg
    17581 tttgcgaaag gcagaaagcg gacatatcaa gctaaaaggg gaagacatca ctaataaaaa
    17641 accccgaaaa atcactgaac atggtgtagg acacgtgcca gaagaccgtc ataaatacgg
    17701 gttagtccta gatatgacat tatctgaaaa tatcgcacta caaacctatc atcaaaaacc
    17761 ttacagtaaa aacggcatgc tgaattattc agtaatgaat gaacatgcca gagaattgat
    17821 cgaagaatat gatgttcgaa caacgaatga acttgttccc gcaaaagctt tatcaggcgg
    17881 aaatcagcaa aaagcaatta tcgctcgtga agtcgaccga gatcctgatc tgttgattgt
    17941 tgcaaatcca actcgtggac tggatgtagg tgcgatcgaa tttattcata aacgtctgat
    18001 cgaacaaagg gacaaataca aagcagtgtt actgatcagt ttcgaattag aagaaatttt
    18061 aaatgtttcg gatcgtattg ctgttatcca tgaaggagaa attgtcggta tcgttgatcc
    18121 gaaagaaaca tctgaaaatg aattaggtct attgatggcg ggctatacct tagaagaagc
    18181 acgaaaagag ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa aaattccgta
    18241 atttattagt gcctgtactt tcagttgttt taggattgat cttaggcgcg atattgatgg
    18301 ctgcttttgg ttttcagccc atccaaggtt attcttctat gctagatgca gcccttggtt
    18361 cgcaaagagg gataggtgaa acgttaaggc aagcaacgcc gttgattttt acagcattag
    18421 gtttttctgt agctaactca gctgggtttt tcaatattgg gctttctgga caagcacttt
    18481 gcgggtggat cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca aaagttgttt
    18541 tgcttccgtt atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg gctgttccag
    18601 gtttacttcg agcatttttt ggaacaagtg aagtgatcgt aacaatcatg atgaactata
    18661 tcttattata cgtaagcaca tttgctttac atgaagtgat gcctgaaagc tatcgctcaa
    18721 gtttggactc ttccaatatg atcagtcaaa atgcctcact gcggttacca tggttaacac
    18781 aagcttttgg cggctctcgc gtaaactcag gattattttt agcaatcatc gcgcttattt
    18841 tcgtttggct gctgatgaag aaaacgactt taggatttga aatccgttct gttggattga
    18901 atccttttgc ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg
    18961 tcatttctgg cgctttggca gggcttggcg gtgtggttga aggattagga acgtatcaaa
    19021 acttctttgt tcaaacgact tcgctatcta ttggatttga cggaatggct gtttccttgc
    19081 ttggttctgg atcagcaatc ggtattcttt tatcagcgat cttgttcagt attttgaaga
    19141 tcggtggact tggcatgcag acaggagcag gtgttccgtt tgagatcgtc aatgtatcga
    19201 ttgctttgat tatcttcttt gtcgggatca acttcctgat tcgtttcgtc atggctaaag
    19261 tcttccaagg gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc
    19321 aaaaccatga agggggcgaa ctataatgtc gactatcgaa ttaattgcct tgatccttac
    19381 ttctacactg gtttattcaa caccgctaat tctaacgtca ctaggcggaa ctttttctga
    19441 acgaagcggg atcgtaaacg tagggctaga gggaatcatg gtcatgggtg cttttagttc
    19501 catcgttttc aacttgacga tgagtccatc acttggttct atgacaccat ggatcgctat
    19561 tttagtgggt ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag caacagtcag
    19621 tttgcgtgct gaccatatta tcagcgggac agtaattaat ctgatggcac ctgctttagg
    19681 tgttttcttg atcaaagcat ggtatggtaa aggtcaaact gataacatca cgcagaatct
    19741 ggggtatttt tctttcccag gattagcaga tattccggtc attggaccaa ttttctttaa
    19801 aaatacattc ttacctgctt attgtgctat tttgattgct gtcctagctt gggtgatcat
    19861 cttcaagacg aaattcggct tgcgcctccg ttcagttggt gaaaatcctc aagctgccga
    19921 cacattaggt atcaatgtat atgctatgcg ttattcaggc gtactgattt ctggcttgtt
    19981 aggcggtatg ggcggtgctg tttttgctca gtcgatttcc ggtaactttt cagctggtac
    20041 gatcgttgga caagggttca tctccatggc tgctatgatc tttggtaagt ggaatccttt
    20101 aggggcaatg ggtgcctcat tgttctttgg ttttgctcaa agcttgagta tcattggcgc
    20161 acaattacca gttatttctt cgattccgcc agtattgctg caaattgctc catatttgct
    20221 gacaatcatt gtgctggttg ttttcttagg gaaagcttct ggtccaaaag caaacgggaa
    20281 gaactatatc aaatcaaaat aatcttttag cgaataaaaa gggtgggtta cgggaagtgt
    20341 ttttcgtctc gccctttttg ttttaaaggc aataaaaata aaaagaaaga aggaataatc
    20401 atgtttaaac gcgtacatct gatcgtcatg gattcagtag gaattggaga agcaccagat
    20461 gccgataagt tcggtgatgt tggttctgat acattaggac acattgcgaa agaagcagga
    20521 ttgaacgttc cacatttaga gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg
    20581 gaatcagtga aagatcatca aggctatgcg acgaaattag aagaagtttc tgtagggaaa
    20641 gatacgatga caggccactg ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg
    20701 tttcctaatg gattccctga tgatctgatc aaacaaatcg aagaattttc tggccgtaaa
    20761 gtcgtatgta acaaacctta tagcggaaca gctgttattg acgattacgg tgaacaccaa
    20821 atgaaaacag gcgatttaat tgtatatact tctgccgatc ctgtccttca gatcgcggca
    20881 catgaagaaa tcattccttt agaagaatta tatcggattt gccaattcgt tcgtgatata
    20941 acaaaagatg atccgtatat gattggccga atcattgcac gtccgtacgt gggtgaacca
    21001 ggagacttca cacgaacaag caatcgccat gattatgcgt tgaatccatt cggacacaca
    21061 gtccttgatt ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa gatcaacgat
    21121 attttcaacg gacaaggaat cactgaatct gtccgcacga aaagcaatat ggatggcgtt
    21181 gaccagctgc tgaaagttat gaaaaacgat ttttcaggaa tcagctttac caatttagta
    21241 gattttgatg cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa
    21301 gaatttgact tacgaattcc agaattgctt gaagcgatgg aagaagacga cttgctaatg
    21361 attacagccg atcacggaaa cgatccgacg tttactggta ccgatcatac gcgagaatat
    21421 gtaccgttat tggtttacag taaaaaaatg aaagaacaag gggcattgcc tcaaggatat
    21481 tattcagata tttcagcaac gattgctgag aactttagtg tttcttcgac tgaaaatggc
    21541 gaaagcttct tgaaattatt aaaataatct ttttaaatag gagaaagcaa atgaccaagt
    21601 taagcgagat gttacaagaa acgacccagt ttatccttga taaaggagta aaagaaattg
    21661 attttggttt gattctaggt tcaggattag gtgaattagc agatgaaatc gaagatgctg
    21721 taatgatttc ttatgatgag atcccgttct ttcctgtttc gacagtagtt gggcatgcag
    21781 gaaagctagt ttatgggtct ctggcaggca agaaagtgtt agccatgcaa gggagattcc
    21841 actactatga aggtcattcc atgcaaacag tgacctatcc agtacgtgtg atggccggac
    21901 taggggccca ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac
    21961 caggagattt gatgctgatt accgatcata tcaattttac tggtgaaaat cctttgatcg
    22021 gtccgaatga agatgcaatg ggccctagat ttccagatat gagtcaagct tatacgcctg
    22081 actatcaagc aacagcaaaa gcagcagcag aaaaactagg tttggatctt aaagaaggcg
    22141 tatatatggg ctattctgga ccaacttatg aaacgcctgc ggagatccga atgtctcgga
    22201 caataggagc cgatgcagta gggatgtcga ctgttccaga agtcatcgta gcagctcaca
    22261 gcggattaaa agtgttagga atctcttgta ttaccaactt agcagcaggt atgcaagcga
    22321 atctgagcca tgaagaagtt gtagaaacaa ctcagagagt caaacaatct tttaaagcat
    22381 tggttaaaga aacattgaca ttattgtaat aggaggaaca ataaaatgag tgtgcatatt
    22441 gaagcgaaag aaggcgagat cgccgataag attttacttc cgggagatcc tttgcgagct
    22501 aaatatattg cggagacttt tttggaagat ccagtttgtt acaatcaggt acgcggtatg
    22561 ttaggcttta ccggcaaata taaaggagaa cgtatttctg ttcaaggaac aggaatggga
    22621 atgccatcag ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg
    22681 attcgagtag gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga tttagtacta
    22741 gcacaaggtg cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt
    22801 cctccaatca gtgatttcaa tctattactg aaagcatatg agatagctaa agaaaagggc
    22861 tatacggttc atgtaggaaa tgttctttcg gaagattctt tctacaaaga tgatttgaca
    22921 gagacgttcc aattagctga acttggcgtt ttaggagtgg aaatggaagc agcggctctt
    22981 tattacttag gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt tagcgaccat
    23041 ttgatcaccg gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc
    23101 gaagtaggat tggaaacagc gatagcagaa taagaaggaa atgaaaaagc tgccctgcat
    23161 taagtaataa gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg gctggactta
    23221 ttattgcaaa tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc tgccgtttaa
    23281 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
    23341 ttgtgaccga gaagttggca ggagaaaacc gtttatacga aaaaagggac tgtgacaaat
    23401 cgtgtcacag tccctttttt atagtgtttt ttataggtag tattttttag caactgttaa
    23461 gtcatcattt aattcataaa caagtggttg acctgttgga atttcaagat ccatgatgtc
    23521 ttcgtcagag ataccttcga tatgtttagc tagggcgcgt aatgagttac catgtgcagc
    23581 tactaaaaca gttttgttgt ctagtaatgc tggagcaatt tcatcttgcc agaatggcaa
    23641 agcgcgttct aatgtcactt tcaagttttc tccaccagga atatcgcgtt tgtctaacat
    23701 tgcgtaacga cggtcatttg ctgcagaacc ttcgtcagtt tcttccatta atggaggaag
    23761 agtatcgtaa gaacgacgcc agatgtgcac ttgttcgtcg ccgtattttt cagctgtttc
    23821 ttttttgttc aaaccttgta atttaccata gtgacgttcg tttaagcgcc atgatttgat
    23881 ttgtggtacc caaagttgat ctgaatattc taagatcaag ttacatgttt tgattgcacg
    23941 agttaaaaca gaagtgtaag caacatcaaa ttcgattcca gcttctttga ttttgcgtcc
    24001 gccttctttt gcttcttcga ttccttctgg tgctagattt acatcagccc aaccagtaaa
    24061 ttggttcaat gcattccatt cactaagacc gtgacgagaa aaaactaatt ttggcatgtt
    24121 tagctctcct tttaagatag gtttatattt ctgaaatttt cagttctgtt ttattgtaca
    24181 attttttgga agagatttcc atcgaaaaat tagataagat tctatgagaa aacaagtaga
    24241 gaatataaaa aaacgaacag tgcccacgca actgttcgtt tttaggtatt agatgcataa
    24301 ttgctcgatt tctttttcag gtaaattgat atatttataa catgttccaa ctgaaacacc
    24361 gcattttgaa gaaataaatt ggatcgtttc ttttttttca taatagagac gtcggatttt
    24421 tttgatcgtt tttgcatcga tttttggtct tccgccgatt tttccttttt tacgagcttc
    24481 atctaatcca actaatgtac gttctttgat caatgcgcac tccatgtttg ctaggttttc
    24541 cattaattgg aaatagattt ttcccatttc gcgcgtatcg attccttcat caagactaat
    24601 caagtgcagc ccgcgatctt tgaataattg cgtgagttct gttaattgcc gcgtagattt
    24661 gcctaatcga tgaagttggc aaatgactag tgcatcgcct tgttgcatgt cagcgatcac
    24721 tgactccaaa tgaacagcag tagaatcatt ttgttccact tcatatgtct catggaaaat
    24781 ttcttgacaa ccaaagtcag ataattgatc aacctgagtt gttaaatcaa cgtcatttat
    24841 ggttgtgcgt gcataaccaa taattttcat ggtgattcct ttctagcagt gattttctaa
    24901 ctggatataa cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt tttcgcaaat
    24961 tttccgctgt ctttttttat tggatgatca agcagttttt ctttatttac gacggattgc
    25021 gtcaagaatc tcaacgcctt tttctgagcc gacaactact ttagaaacca tgtttaggaa
    25081 aagcccatgt tctactaccc caacaagttc atctaaatag gtagctaagc tttctggctg
    25141 ttcgatgact tctagatgca gatcaatgat gtaatgacca ccatctgtgg ttaagacttc
    25201 tccgttttcg tctgtacgca agacgggttg gaatcttttt tcttcaaaaa tatgaagtag
    25261 ctgttgggag ccataaggga tcacttcaac tgggagtggg aacttcccaa gtttatcgac
    25321 taatttagaa gaatcaacga tccaaatcgt ttcttttgag tacgtagcga ctattttctc
    25381 gaaaagcaaa gcagcgccgc cgcctttgat cccttgaaaa tcagcactga tttcatcagc
    25441 accgtctatt gtcaaatcga ctacggggac ttcatcaata ctctttaacg gtattcctaa
    25501 tttttccgct tgttcttttg tcgcatttga agtagtaact cctgtgatac gtaaaccttc
    25561 ttcacgcata cggcgaccaa tctcttctac catataatag gcggttgaac cggtcccgag
    25621 acctacgatc atcccgtcct ttacgaattt cgcggcttca atgccagcta attgttttaa
    25681 attcatcttt gcgtccacct ttttttagtt tgagcatcgt gctttcctca ctattttagc
    25741 gtgatttacg gtaaaaagat accattcttt tttattccaa taaagctttt tcaaaaaaaa
    25801 ttgcatctct tgttgacatg ttttttcatt cgtgatattc taacaaaggt gctttatcta
    25861 caggtttctg tgaagaaacg tgctgactgt ttatcaaagc atatttgata aggatgcaga
    25921 cattgcattt attttttatc acaaaaaaag aaccacctgg atgtgtggaa ctagacgcaa
    25981 atcgtgggaa ggaggaaaca aggaatgcct acaattaacc aattagtacg taaacctcgt
    26041 aaatcaaagg tggaaaaatc taattcacct gctttgaaca aaggatataa cagctttaag
    26101 aaaactcaaa ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg tgtgggtaca
    26161 atgacaccta aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg tttgtcaaac
    26221 ttaattgaag ttactgctta tatcccaggg attggtcata acttacaaga acacagtgtt
    26281 gtattattac gcggtggtcg tgtaaaagac ttaccagggg tacgttacca tatcgttcgc
    26341 ggtgctttag atactgctgg tgttaacgac cgtaaacaaa gccgttctaa atacggaact
    26401 aaaaaaccta aagcttaatt ttaattatta aacaaatgaa acttgcttct actatataga
    26461 agtaattact atgaacaata tttcggaagg aggagttacg gatgccacgt aaaggtcctg
    26521 ttgcaaaacg tgatgtttta ccagatccta tttataactc aaaattagta actcgcttga
    26581 tcaaccgtgt aatggttgac ggaaaacgcg ggattgcagc taacattatc tataatgcct
    26641 ttgacttgat caaagaatct acaggtaacg atccattaga agtgtttgaa caagcaatga
    26701 aaaacgttca acctgttctt gaagttaaag cacgtcgtgt tgggggttca aactaccaag
    26761 taccagttga agttcgccct gaacgccgcg ttactctagg tcttcgttgg gtagtaaact
    26821 acgctcgctt gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa atcatggatg
    26881 ctgcaaacaa cacaggtgct tctgttaaaa aacgtgaaga cactcataaa atggctgatg
    26941 ccaaccgtgc gtttgctcac tatcgctggt aagattctct ctgtttggtc ggcttttata
    27001 agtcgacctc agatagtgtc cttattttta gcaactagat agtaaagaga ggagaaacaa
    27061 ggagaatgac cagagaattt tctttacaaa acactcgtaa tatcggtatc atggcccaca
    27121 tcgatgctgg taaaacgact gctactgaac gtatcttgta ctatactggt aaaatccata
    27181 aaattggtga aactcatgaa ggagcttcac aaatggactg gatggaacaa gaacaagaac
    27241 gtggtattac gatcacttct gctgctacta cagcacaatg gaagggtcac cgaatcaaca
    27301 tcatcgacac accaggacac gtagacttca cagttgaagt tgaacgttca ttgcgtgtac
    27361 ttgatggtgc tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa actgaaacag
    27421 tttggcgtca agcaacaact tatggtgtac ctcgtatcgt atttgcaaac aaaatggaca
    27481 agatcggtgc tgacttctta tattcagtat caactttaca tgaccgtttg caagcaaacg
    27541 ctcacccaat ccaattacca attggtgcgg aagacgactt cacaggaatt atcgaccttg
    27601 taacaatgaa ggctgaaatg tatacaaacg atcttggaac agagatcgaa gaaacagaaa
    27661 ttcctgaaga ataccgcgaa ttagctgaag aatggcgcga aaaattggtt gaagctgtag
    27721 ctgaaactga tgaagaactt actttgaaat acttagaagg tgaagaaatc actgaagctg
    27781 agttaaaaga aggtatccgc cgcgcaacag ttaacgtaga attctaccca gtactatgtg
    27841 gttcagcatt taagaacaaa ggggttcaat tactattgga cgctgttctt gattacttgc
    27901 catcaccgtt agatatccca gctatcaaag ggattgaccc taaaactgat gaagaagtag
    27961 aacgtcctgc agacgacagc gctcctttct cagctttagc atttaaagtt atgactgacc
    28021 cattcgtagg tcgtttaact ttcttccgtg tgtactctgg tgttttacaa tcaggttcat
    28081 acgtacaaaa cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta cagatgcatg
    28141 ccaactctcg ttcagaaatc tctgaagttt acgctggaga tatcgctgct gctgttggtt
    28201 tgaaagacac aactactggg gacacactat gtgatgaaaa gagtctagtt attcttgaat
    28261 ccatggaatt cccagaacca gttatccaag tcgctattga acctaaatca aaagctgacc
    28321 aagataaaat gggtgtggct ttacaaaaac tatctgaaga agatcctact ttccgtgctg
    28381 aaacaaacgt ggaaacaggc gaaacaatca tcgctggtat gggtgaactt cacttagata
    28441 tcatcgttga ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt gcgcctcaag
    28501 tatcttaccg tgaaactttc cgtgctggca ctcaagccga aggtaagttt gtacgtcagt
    28561 ctggtggtaa aggtcaatac ggtcacgtat ggattgaatt tacaccaaat gaagaaggtg
    28621 ctggtttcga atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa tacattccag
    28681 ctgttgaaac tggtttgaaa gatgctatgg aaaatggtgt cttagctggc tatccattag
    28741 tagatatcaa agctaaactt tacgatggtt cataccatga tgtcgattca aatgaaactg
    28801 ccttccgtgt tgctgcttct atggcattac gtgcagcagc taagaaagct aatccagtaa
    28861 tccttgaacc aatcatggct gtagaagttg ttatccctga agattattta ggtgatgtta
    28921 tgggtcacgt aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc gctggcggcc
    28981 aacaagttgt tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct acaactcttc
    29041 gttctgctac tcaaggtcgt ggtacattta caatgacatt tgaccactac gaagatgtac
    29101 caaaatcagt acaagaagaa atcattaaga aaaacggcgg aaaagctgaa taatttcttg
    29161 aaaccggata gtcctgcaaa gagattatcc aaagagacta tatttttaat atagtttcgt
    29221 gtaaaataat tatcgatgat atgggcatgt ggtgtacctt gtgtacatca tatacctatc
    29281 agacaaataa aaaatatatt gtatttttag gaggacattt taaaatggca aaagaaaaat
    29341 ttgaccgttc taaaccacac gttaacattg gtactatcgg acacgttgac catggtaaaa
    29401 ctacattaac agctgcaatc acaactgtac tatctaagaa aaacggcggc caagctatgg
    29461 cttacgatca aatcgatggt gctccagaag aacgcgaacg cggaatcaca atctcaacag
    29521 ctcacgttga gtatgaaaca gatacacgcc actacgctca cgttgactgc ccaggacacg
    29581 cggattacgt taaaaacatg atcactggtg ctgctcaaat ggacggagct atcttggtag
    29641 tttctgctgc tgacggccca atgcctcaaa ctcgtgaaca catcctattg tctcgtcaag
    29701 ttggtgttcc ttacatcgtt gtattcttga acaaagtaga catggttgat gacgaagaat
    29761 tactagaatt agttgaaatg gaagttcgtg acctattaac agaatacgaa ttccctggtg
    29821 acgatgttcc tgtagttgct ggatcagctt tgaaagctct agaaggcgac gcttcatacg
    29881 aagaaaaaat tcttgaatta atggctgcag ttgacgaata catcccaact ccagaacgtg
    29941 acaacgacaa accattcatg atgccagttg aagacgtgtt ctcaattact ggacgtggta
    30001 ctgttgctac aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa gttgaagttg
    30061 ttggtattgc tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg ttccgtaaat
    30121 tgttagacta cgctgaagct ggagacaaca ttggtgcttt actacgtggt gttgcacgtg
    30181 aagacatcca acgtggacaa gttttagcta aaccaggtac aatcacacct catacaaaat
    30241 tctctgcaga agtatacgtg ttgacaaaag aagaaggtgg acgtcatact ccattcttca
    30301 ctaactaccg tccacaattc tacttccgta caactgacgt aacaggtgtt gttgaattac
    30361 cagaaggaac tgaaatggtt atgcctggcg acaacgtaac aatggaagtt gaattaatcc
    30421 acccaatcgc tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga cgtacagttg
    30481 gtgccggtgt cgttacagaa atcaaagctt aattcactta agttttaaag taaagcaaca
    30541 tttattcgga cgtaatcgct cggaacgaag gtcttaggat ctttgttccg agctttttta
    30601 tttattgcag gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa
    30661 agagtcagta ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc
    30721 cttgctttgt gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc
    30781 acagacgtaa tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac
    30841 gctttgccac gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata
    30901 ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa
    30961 caccgtattt tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct
    31021 agcgtgtctg gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg
    31081 actcataaat acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc
    31141 gacattgtga acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct
    31201 ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg
    31261 accaaaggaa tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa
    31321 ttaattccag taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg
    31381 gaaacggatg gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca
    31441 aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt
    31501 gaattcaaaa atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt
    31561 ttccaagcag gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg
    31621 gttatcaaac gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt
    31681 cgtcctgggt caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct
    31741 ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca
    31801 gaaagaaatg ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact
    31861 atcaaatcag ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg
    31921 aatgtagcat tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa
    31981 atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg
    32041 tcattaagac aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt
    32101 aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa
    32161 tggcgtggag gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct
    32221 aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac
    32281 ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt
    32341 cttgctaact tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt
    32401 gctgctttat cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt
    32461 gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt
    32521 gaggaggtgc ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa
    32581 aatcaatgct tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca
    32641 aaactttagt aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca
    32701 tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac
    32761 gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg
    32821 ctgaataagc actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa
    32881 gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac
    32941 aacatcaaca cctgaaaaat cattgttaca accattgaaa aaccatgctg gacgtaacaa
    33001 caacggccgc atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat
    33061 cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc
    33121 aaaccgttca gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt
    33181 agcacctaaa ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa
    33241 agtcggaaat gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga
    33301 aatgaaacct ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact
    33361 tggtaaagaa ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt
    33421 agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat
    33481 cggtaaagct ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat
    33541 gaaccctaat gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc
    33601 accagtttct ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa
    33661 atcagacaaa cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc
    33721 acattttgag aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga
    33781 gcatttaatg aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac
    33841 ttggtcacgc cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga
    33901 tggacgtaaa catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga
    33961 atttgcacct acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta
    34021 atttgagggg aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa
    34081 aaacagttcg cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa
    34141 gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg
    34201 aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa
    34261 acttggtagt aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc
    34321 gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat
    34381 cagaaaaata aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta
    34441 ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta
    34501 cacgaagatt taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca
    34561 acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca
    34621 ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa
    34681 ttaactggta aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca
    34741 aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct
    34801 caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta
    34861 tctggtcgtc taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt
    34921 cctcttcata ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac
    34981 ggaaaactag gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac
    35041 actgagaaag gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat
    35101 tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg
    35161 gtttgcaagc tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg
    35221 caatgactcg ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat
    35281 cttatactgc caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat
    35341 gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag
    35401 tagctcgtga agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa
    35461 aacgtgagga aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact
    35521 gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa
    35581 ttagcaacag gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca
    35641 cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt
    35701 atgactgaag gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg
    35761 gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc
    35821 atgaactatt ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac
    35881 atcgtgagaa ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa
    35941 gtagtcgaag aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca
    36001 aaatctgaaa ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat
    36061 aactcaggcg cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact
    36121 gctaacatcg gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc
    36181 aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca
    36241 gacggttcat atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc
    36301 cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg
    36361 aagatcgttt cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc
    36421 gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa
    36481 aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt
    36541 gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt
    36601 gagatggaag cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct
    36661 ggccgagttg gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt
    36721 gaagttttag ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa
    36781 gaaaaatatc ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt
    36841 atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca
    36901 aacgctaaaa acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca
    36961 atgattacaa aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt
    37021 ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct
    37081 gtttcactac cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt
    37141 ggaaactata ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta
    37201 gtagataaag tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa
    37261 tctcgtgagt tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac
    37321 tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa
    37381 gcttatactc gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt
    37441 tgccgtattt gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct
    37501 agctggtaaa aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc
    37561 caattgcaga ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag
    37621 aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg
    37681 ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat
    37741 ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc
    37801 gtgcttatgt caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca
    37861 tttcgacttc tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg
    37921 aagttatcgc ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc
    37981 cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac
    38041 aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa
    38101 atgaatatcg aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa
    38161 acaattcatg gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt
    38221 ttccaaaaag cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa
    38281 cttgtattga acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact
    38341 gttgaagtac cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc
    38401 gaattagctg caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt
    38461 cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga
    38521 aaaattgcat agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa
    38581 aatacatttt tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata
    38641 gtagtacaat aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac
    38701 acgtcaaaag agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg
    38761 cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg
    38821 tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga
    38881 agcagcacaa gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt
    38941 agtagtcttt gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc
    39001 tgctcgcgaa aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc
    39061 gatccaaaac atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt
    39121 gttaaaggtg gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga
    39181 cacgtaggat ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt
    39241 gaagatgcga agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa
    39301 gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg
    39361 gttgctgctg gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact
    39421 tctaaatctt taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg
    39481 aaacaattga aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta
    39541 atcggataag gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta
    39601 tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca
    39661 gttcagtggt taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc
    39721 tagtggacgt tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc
    39781 aaacctatga aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt
    39841 gttggtcgtg gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa
    39901 aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc
    39961 cgtcgtttac caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat
    40021 cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa
    40081 gctggaatcg tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac
    40141 aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg
    40201 gctggtggat caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa
    40261 aagacattag atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg
    40321 cacatattac tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat
    40381 ttttcaacat gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa
    40441 tgggggtttc accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg
    40501 tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag
    40561 cgactcgata tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat
    40621 tcaatcaata cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga
    40681 tcggtttgat tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag
    40741 aaaaaggtat cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc
    40801 ctgaaagtat cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat
    40861 ggaaatctgc tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg
    40921 taacattttt ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg
    40981 gcgcgcctac aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta
    41041 tctttgctag ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat
    41101 tcggttctga aaactggtac aaagtgatga tggagatctt taactataat acagcacctg
    41161 gtgctacgat ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag
    41221 taaaccctga gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac
    41281 gccctggtaa ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg
    41341 gtgcagtctt ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc
    41401 ttccacaatc aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag
    41461 aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt
    41521 aagaaagcgt gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa
    41581 acaatgaacc tcattttaat ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa
    41641 atcattgatg cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg
    41701 caaaacgaaa cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt
    41761 cctgatgaag taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa
    41821 ggcttcttat tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg
    41881 ctaaaagatt tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc
    41941 ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa
    42001 attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat
    42061 caaagagaag atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat
    42121 agtgaaccaa tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct
    42181 cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc
    42241 ccgtagccga cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata
    42301 tgtcagtccg acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga
    42361 actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg
    42421 cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc
    42481 aatgtttaaa gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat
    42541 tcgtatgcat tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata
    42601 cgatcttaac cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca
    42661 ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta
    42721 attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt
    42781 caaggataat tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg
    42841 ataaacgtgt agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa
    42901 agatcttagc tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac
    42961 aaacagatgc tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg
    43021 aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc
    43081 gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc
    43141 cagctcgcgc tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca
    43201 tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg
    43261 cacatatcca ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg
    43321 cattagcgtg gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt
    43381 ttgccgctca aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga
    43441 ctgttgaagt aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac
    43501 aagctacagg tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg
    43561 cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta
    43621 acgtcattga acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc
    43681 gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa
    43741 cctcttgaaa gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct
    43801 tctttgccag gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct
    43861 accatcaaag gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg
    43921 ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca
    43981 gtaactgctg gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac
    44041 atctgtagtg tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt
    44101 ggttatgttc aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc
    44161 gattcaatct acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga
    44221 caccgtgaag attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg
    44281 ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac
    44341 ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa
    44401 aaaatgttgg aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg
    44461 aaacgtgcag gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc
    44521 aaagtccgta acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta
    44581 ggtttaggtt tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt
    44641 tatcgtaaac taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact
    44701 gatttattaa tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca
    44761 actgcagaaa aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct
    44821 gcggctttcg tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt
    44881 gaatcagctt tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc
    44941 ggatacacac gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc
    45001 ttagaacttg tctaaaatca cactaataac atcactttat agatgattct tttagagtgt
    45061 tatgatgttg gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg
    45121 cttggggagt aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac
    45181 agatatgttt gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt
    45241 tttttgatag tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat
    45301 tgaattagaa aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga
    45361 tgtctccttt acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg
    45421 aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa
    45481 agtgggaaat caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat
    45541 ggtttttcaa aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt
    45601 tggtcttgaa aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact
    45661 tgagaaagtt agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca
    45721 aaaacaacgt gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga
    45781 tgaagcaacc agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa
    45841 gatcaaagaa gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc
    45901 taatgccaat cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa
    45961 agaaattttt tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga
    46021 aaaactgaag agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga
    46081 agaaaggatg gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag
    46141 ccgaatacac catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac
    46201 agttatactg cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg
    46261 aatgcgcttg ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg
    46321 gatacggata ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt
    46381 ccagaagcac agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac
    46441 tttggtgtca gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg
    46501 ctggatgaaa gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga
    46561 gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg
    46621 gcaggacttg atccccaggg cagaaaagaa atgatggaga tgttttggcg tcttcataag
    46681 gaacagcaga tcactattgt attagttacg catttaatgg atgatgtagc caattttgca
    46741 gattatgtct atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc
    46801 tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt
    46861 gcagaagaat taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa
    46921 ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt
    46981 tggtcgttat atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt
    47041 agccagtttt tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt
    47101 gttggcattt ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat
    47161 ccgaggcatt cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt
    47221 tacaagtggc ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt
    47281 gatgaacggc ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact
    47341 cacgcttact acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt
    47401 gaaagccgta cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt
    47461 tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt
    47521 tgattttggt gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc
    47581 tttatttgtc agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg
    47641 ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac
    47701 gatggttatc attgcttttg tattattaac tgtgctactg atctttttaa gaggataaac
    47761 gttcagtggg ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcaagc
    47821 agtgtatgac aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt
    47881 atctgaaaag aaagaacgaa aaa
//
LOCUS       DAZKOU010000011        46565 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000011 DAZKOU010000000
VERSION     DAZKOU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.011"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..932
                     /locus_tag="ACG827_000584"
     CDS             51..932
                     /locus_tag="ACG827_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0482750.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            945..2555
                     /locus_tag="ACG827_000585"
     CDS             945..2555
                     /locus_tag="ACG827_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0482751.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2632..3291)
                     /locus_tag="ACG827_000586"
     CDS             complement(2632..3291)
                     /locus_tag="ACG827_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0482752.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3574..4239)
                     /locus_tag="ACG827_000587"
     CDS             complement(3574..4239)
                     /locus_tag="ACG827_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0482753.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4386..4991)
                     /gene="dhaL"
                     /locus_tag="ACG827_000588"
     CDS             complement(4386..4991)
                     /gene="dhaL"
                     /locus_tag="ACG827_000588"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0482754.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5019..6029)
                     /gene="dhaK"
                     /locus_tag="ACG827_000589"
     CDS             complement(5019..6029)
                     /gene="dhaK"
                     /locus_tag="ACG827_000589"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0482755.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6004..6399)
                     /gene="dhaM"
                     /locus_tag="ACG827_000590"
     CDS             complement(6004..6399)
                     /gene="dhaM"
                     /locus_tag="ACG827_000590"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0482756.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6553..6873
                     /locus_tag="ACG827_000591"
     CDS             6553..6873
                     /locus_tag="ACG827_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0482757.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6985..7842)
                     /locus_tag="ACG827_000592"
     CDS             complement(6985..7842)
                     /locus_tag="ACG827_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0482758.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7950..9254)
                     /locus_tag="ACG827_000593"
     CDS             complement(7950..9254)
                     /locus_tag="ACG827_000593"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0482759.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9251..9760)
                     /locus_tag="ACG827_000594"
     CDS             complement(9251..9760)
                     /locus_tag="ACG827_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482760.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9935..12070)
                     /locus_tag="ACG827_000595"
     CDS             complement(9935..12070)
                     /locus_tag="ACG827_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0482761.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12153..13808)
                     /locus_tag="ACG827_000596"
     CDS             complement(12153..13808)
                     /locus_tag="ACG827_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0482762.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13810..14124)
                     /locus_tag="ACG827_000597"
     CDS             complement(13810..14124)
                     /locus_tag="ACG827_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0482763.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14294..14554
                     /locus_tag="ACG827_000598"
     CDS             14294..14554
                     /locus_tag="ACG827_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0482764.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14568..14882
                     /locus_tag="ACG827_000599"
     CDS             14568..14882
                     /locus_tag="ACG827_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0482765.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15612..16166)
                     /locus_tag="ACG827_000600"
     CDS             complement(15612..16166)
                     /locus_tag="ACG827_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0482766.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16292..18221)
                     /locus_tag="ACG827_000601"
                     /pseudo
     CDS             complement(16292..18221)
                     /locus_tag="ACG827_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18527..19243)
                     /locus_tag="ACG827_000602"
     CDS             complement(18527..19243)
                     /locus_tag="ACG827_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0482767.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19244..21067)
                     /gene="glpO"
                     /locus_tag="ACG827_000603"
     CDS             complement(19244..21067)
                     /gene="glpO"
                     /locus_tag="ACG827_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0482768.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21081..22577)
                     /gene="glpK"
                     /locus_tag="ACG827_000604"
     CDS             complement(21081..22577)
                     /gene="glpK"
                     /locus_tag="ACG827_000604"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0482769.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22713..24173)
                     /locus_tag="ACG827_000605"
     CDS             complement(22713..24173)
                     /locus_tag="ACG827_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0482770.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24163..25458)
                     /locus_tag="ACG827_000606"
     CDS             complement(24163..25458)
                     /locus_tag="ACG827_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0482771.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25790..26467)
                     /locus_tag="ACG827_000607"
     CDS             complement(25790..26467)
                     /locus_tag="ACG827_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0482772.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26497..27588)
                     /locus_tag="ACG827_000608"
     CDS             complement(26497..27588)
                     /locus_tag="ACG827_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0482773.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27689..28123)
                     /locus_tag="ACG827_000609"
     CDS             complement(27689..28123)
                     /locus_tag="ACG827_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0482774.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28707..30164)
                     /locus_tag="ACG827_000610"
     CDS             complement(28707..30164)
                     /locus_tag="ACG827_000610"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0482775.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30161..32056)
                     /locus_tag="ACG827_000611"
     CDS             complement(30161..32056)
                     /locus_tag="ACG827_000611"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0482776.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32114..33889)
                     /locus_tag="ACG827_000612"
     CDS             complement(32114..33889)
                     /locus_tag="ACG827_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0482777.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33939..35435)
                     /locus_tag="ACG827_000613"
     CDS             complement(33939..35435)
                     /locus_tag="ACG827_000613"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0482778.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35629..37251)
                     /locus_tag="ACG827_000614"
     CDS             complement(35629..37251)
                     /locus_tag="ACG827_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0482779.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37579..38892)
                     /locus_tag="ACG827_000615"
     CDS             complement(37579..38892)
                     /locus_tag="ACG827_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0482780.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39137..39361)
                     /locus_tag="ACG827_000616"
     CDS             complement(39137..39361)
                     /locus_tag="ACG827_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482781.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39381..39803)
                     /locus_tag="ACG827_000617"
     CDS             complement(39381..39803)
                     /locus_tag="ACG827_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0482782.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39945..40655)
                     /locus_tag="ACG827_000618"
     CDS             complement(39945..40655)
                     /locus_tag="ACG827_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0482783.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40673..41170)
                     /locus_tag="ACG827_000619"
     CDS             complement(40673..41170)
                     /locus_tag="ACG827_000619"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0482784.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41207..41659)
                     /locus_tag="ACG827_000620"
     CDS             complement(41207..41659)
                     /locus_tag="ACG827_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0482785.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41794..42399)
                     /locus_tag="ACG827_000621"
     CDS             complement(41794..42399)
                     /locus_tag="ACG827_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0482786.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42849..43469)
                     /locus_tag="ACG827_000622"
     CDS             complement(42849..43469)
                     /locus_tag="ACG827_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0482787.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43471..44340)
                     /locus_tag="ACG827_000623"
     CDS             complement(43471..44340)
                     /locus_tag="ACG827_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0482788.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44363..44770)
                     /locus_tag="ACG827_000624"
     CDS             complement(44363..44770)
                     /locus_tag="ACG827_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0482789.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44789..46078)
                     /locus_tag="ACG827_000625"
     CDS             complement(44789..46078)
                     /locus_tag="ACG827_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0482790.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            46136..>46565
                     /locus_tag="ACG827_000626"
     CDS             46136..>46565
                     /locus_tag="ACG827_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0482791.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT        13115 a         9903 c         7986 g        15561 t
ORIGIN      
        1 ctatttaatc ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa
       61 tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct
      121 ctttcaccgt acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat
      181 ccacgacgat tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt
      241 ttgggcaaga tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag
      301 gcgatatctc tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc
      361 ttcatggaaa agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc
      421 ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg
      481 ctttggctgt tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt
      541 tgatggaaac gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc
      601 tactttcttc tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac
      661 gcgaaggaac actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga
      721 ccatcactat agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg
      781 actttgtgca aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga
      841 ttttagctga agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg
      901 aggatttatt catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt
      961 cacgctggtc cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat
     1021 ggctagtcgg tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt
     1081 ccggacaagg attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg
     1141 tcggaccaac tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg
     1201 aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc
     1261 aaacgagaaa aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac
     1321 gtcaagcaaa ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa
     1381 tatcgatcag ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt
     1441 ggctttttgg gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg
     1501 ctgcacaact gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg
     1561 gactttacct tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc
     1621 ctttaggctg gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt
     1681 atttgttttt gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag
     1741 atttgaacac cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt
     1801 tatccttgcg tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca
     1861 cttttggttt gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag
     1921 aaagtaacga cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact
     1981 ttacaagcac gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca
     2041 tcaatcgttt atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa
     2101 tttctaggaa ccaactttat tggaccaata tgttactagc tattctattt gctatattgg
     2161 caatcctgat tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga
     2221 aagacatgga tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag
     2281 tgtttatcgg aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt
     2341 atatctactt agcttactcc tttgcactca attactttgg cggaattttg gatttaccag
     2401 aatggttttc caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg
     2461 atctttccgt gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta
     2521 ttggttatag gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc
     2581 tagcctccaa atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc
     2641 ttctaactgc tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt
     2701 ctcctctaat aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc
     2761 aagttctttc attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc
     2821 aagcagatag acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg
     2881 ctgtactttt gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata
     2941 atgcagtaag agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag
     3001 tgcttcgcca aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc
     3061 gcccggctct attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt
     3121 catactgccg gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc
     3181 tcctttttcc acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac
     3241 taagtgattg aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc
     3301 tccttctaaa cagctatttc taataaagaa acataacttc ccttattcca actgataatt
     3361 ttcttttatc atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct
     3421 ttttgtccct tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt
     3481 ttttaaatat ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa
     3541 ggtttgtttt tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt
     3601 ttgatatagt ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat
     3661 ttcttgcgga tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt
     3721 caactcttct tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag
     3781 ttcatgttct ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc
     3841 tttcagtatc gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt
     3901 ataatgttgg tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca
     3961 ggcattctcg tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt
     4021 ccagccattc gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag
     4081 tgcagctgtc tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt
     4141 gatttgttca atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg
     4201 tcgatcaaac aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat
     4261 cttttgtatt ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc
     4321 tgcatcaagt cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat
     4381 tgaacttaag gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca
     4441 gctcctgggt cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta
     4501 gctaccaaga ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct
     4561 gccttcaatg cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct
     4621 gcctttcctc ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct
     4681 tgagaatcaa tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg
     4741 tataacggac cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat
     4801 gtatcgctga ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct
     4861 cttcccatgt tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat
     4921 tgtttgttct gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg
     4981 gttagtttca tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca
     5041 ttgctttcta aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga
     5101 ccttgcatat ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct
     5161 ctttcttcca acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc
     5221 ccgcccattc cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa
     5281 atcttagtaa caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag
     5341 cctggctcac cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct
     5401 ggtttcccta cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc
     5461 tgtacgacct tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca
     5521 tcgcccacga ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac
     5581 gtactatttt caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc
     5641 atagcagcta attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg
     5701 atcagcaaaa ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga
     5761 gttggagaag taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac
     5821 ccggcatggg atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct
     5881 ttctgattct tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt
     5941 tttactaatc cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc
     6001 aatttattcc catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc
     6061 atctgttata ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac
     6121 cagatgataa cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa
     6181 taccgcgcta ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac
     6241 tgcttctacg attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa
     6301 aatttgcacc tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac
     6361 tttttgacta tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac
     6421 atagttgcta acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt
     6481 ctaattttgg gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag
     6541 aggagtgatc gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct
     6601 tataaaggga tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag
     6661 gcggggttgc gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt
     6721 gcaagagaag aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg
     6781 atcattggcg taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc
     6841 acatgtagtg gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa
     6901 agagagatgg gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta
     6961 ctctaccttc ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc
     7021 gactaacaca cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt
     7081 tggcaaagtc tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt
     7141 gcttccagag gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa
     7201 actagctgct aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct
     7261 gatctttccg tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt
     7321 tccagaagct gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat
     7381 cttcccgtca agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt
     7441 ctcaagttgt gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat
     7501 tgctgcttgg tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt
     7561 tcttttattt tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat
     7621 tgatgtatct tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt
     7681 catttctgtt tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc
     7741 tgtttgagca ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga
     7801 aacagcaatt ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac
     7861 tactttttaa ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca
     7921 ggctgtttgt ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat
     7981 gtcccgcttc cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt
     8041 atcgcgttca ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat
     8101 tttcttcgtc gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt
     8161 tcaaagcaga tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct
     8221 tgatatacat aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt
     8281 gaccagacaa tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa
     8341 ggatggagca tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa
     8401 cgttttctgt gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc
     8461 tcaggagcaa aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct
     8521 tctgtcgctc gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc
     8581 gtatttgctc cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg
     8641 atgcccacga tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt
     8701 tggacatcca tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa
     8761 ttcacatacc taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc
     8821 aatttccctt ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt
     8881 tcgatccgaa ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt
     8941 gtttggtccg ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc
     9001 gtcatcaaat actcgattgt atattcgatc acaatttctt cattatgggc tggcaccatg
     9061 atcgtgatga acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg
     9121 tataaaaaca cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa
     9181 gaaacgagaa aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg
     9241 gtgattgaga tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg
     9301 tatctagatt tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg
     9361 ttccaaaacg ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat
     9421 cttcatattc aaaagattta tttacttttt tgtatagatt tcgattgttc cacctagtca
     9481 atacaaatga ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg attaagaaat
     9541 acagcatttt cttttctgat aaaaaagcgt acaaggaaaa atccttggct ctttctggtc
     9601 tcgttaatgg gaaaaagacc catataaaag gaagaacaac gatcatccat cctaaaagag
     9661 ccactaacgt ttgccaaatc tttaaccagt aattaccttt tgaaaaatac ttatctttca
     9721 ccaaaattga tgcaacttct tctcttgtta cttttttcat atcctcacct cacgtttttt
     9781 tgttacgacg taggagttta ccatacattt catttctttt cacataaagt gttcctaaat
     9841 ttttcctaag aaaaagaaca aaacatttct catcagcaaa tgaaaaatat tttgttcttt
     9901 ttatcagttg ttctttttat tttttatctc ttaattatgt tgctctttcc aatacgacaa
     9961 tgcttttcat tcctctagat atcgccgtgt acatttgtct gcgtcttctt tgctcatctc
    10021 catcgttttc aaatggatat aagattacat gatcaaattc cagtcctttt gctagactca
    10081 aaggaatgat ttggcagttt tctaatccat atgaattttt tttaatcgat tcttgtagcg
    10141 tttgggtttc ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt agagaaacca
    10201 aaatctcagc tagtattttc aacaattctt cagaggatcg aaaagagcgg tattcgggtt
    10261 cttttccttg cggtcggatc gatacgatgt cgatcttctc ttgatccaca gcataggttt
    10321 tgaaaagctc tgtgattgct ccgctagaac ggtaactatt tctaagatca taccgatgga
    10381 tcggcagatg gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg gaagaattaa
    10441 agattgcctg attctcgtca cctactaaag taaagtgagt ttttggaaat aattcgatca
    10501 atagacctaa ttgtgccagg gtataatctt gtacttcatc aatcaataga tagcgaaatg
    10561 ctggtacatt taccttttca acaagtaaat gacgaatagc taatataatg actgcttcat
    10621 caacagtacg tgtgggttgc caagcataag ctgagccata tcgtttttca taaattcttt
    10681 caaacaactg atgctcatcc acccatgcca tttcttcgat ttgtctggtt atttttcgat
    10741 attttttttg taaaagtttc cttgcataag ctacaatact ttgttccgaa tcatcagaaa
    10801 tcaatttacc gaataattcc aactgcctat cttctgtcaa cgagagtacc tgattctgca
    10861 cagcaggact ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc agtgcctttt
    10921 tcgttgcttg aagcctatcg atcaatttag gatgagcagg aactttctca tatatttttt
    10981 cgatcgtttt tttagaaagg actgtttttc catttttagt aagtccacgg ataaaattcg
    11041 gatgatctaa aaacaaagga tctgattgtt ttagattatc gataaacttt ttgctacgca
    11101 aacgttctgt ctgctccgat acattttctc ccgtgatacg tttgaaataa gcctcttcac
    11161 cttctatttc ctctgctgaa agctgtgaaa ccagctgtat gatggttaga ttcagtggat
    11221 ttctttcacc taatgatggt aggacattcg aaatatagtc gataaaccga tgattaggtg
    11281 atagaatcag caagtcatcg gatgtcatcg tatctcgata taaatagagc agatacgcaa
    11341 tacgctgcat gatagcagag gttttaccac ttcctgctac gccatttacc aagatatttt
    11401 gacttttcac atcccgaatg attgtgtttt gttccgattg gatcgtagca gtgatagcct
    11461 tcatttcatt ggtctcatct tggcctaaga cttctaataa tacgtcatct tgaatagcta
    11521 cagacgtatc aaaaaacttg accaaacgat ctctttccaa aagaaattgt cgtctttctt
    11581 taataaccgt atcgatttta ttttgacgaa ccgtataaga agatggacca atcatgttat
    11641 tgtaaaaaag ttcggcaatt ggtgaacgcc aatcataaac aagattttca tctgcatcat
    11701 ctgtgaaatt tgctgtaccg atataaaagg attcttttgg ctcattatcc agaaagtcta
    11761 caacgacttt tccaaagtag ggacttttca ataaacgttc gatctttgct agctgatttt
    11821 ctgcgttttt ctgtttgata ttcatctgat cgatttctct atttttcatt tcaatcatcg
    11881 aaaaagtatc caggttatct aagtaactgt caaaattcaa tcgaagctcg ccagccattt
    11941 ctttcaacga ctggtttcct tctatttttg ccgtatccat cgattcccgc agttcgtttt
    12001 ttttatcaat tagcttttga tacacgttct tcagatatgc ttcttctaac tgtttttgtg
    12061 tgttgaccat acacattctc ctcttttttt aaagtggtgg aaccgattag tttacctctt
    12121 ttaagagtcg aatgcaagct ttttaacgag tatcatttgt taggatatat cagttcttca
    12181 ggtcggacag tttgatataa agaaaatgca ccatcaaggt tttttacgtg gtagccagac
    12241 tgcttcaaga ttctttccgc tagatagcta cgtaatccgc tatgacagct gacgatatat
    12301 tcttgttggc tgtctaattc atttaatcgg tcccgaagtt cattcaaagg gatatgtttg
    12361 gcttccttga atttgccctt ttccaattct tctgctgttc gcacatctaa taaaattttt
    12421 ccttttgcta gttctgtcgg caattcatgc cattgtacgg tttcactaag cccttctgca
    12481 atgttcattg ctgcatatcc tagcatgttt actggatctt tggctgagcc aaatggcggt
    12541 gcatacgtca attctaattc cggtaaatcg aagatcgtca aatgcccttt gattgctgtt
    12601 gctaaaatat cgatccgttt atccacacct tttgccccaa caccttgtgc accataaatc
    12661 tcgccagtct ctggatggaa gatcaacttc agtaaaatat ctgttgcttc tggataatag
    12721 cttgcatgat cttttcctga tacatgaatg acttcaaaag acagttcgtt ttctttggct
    12781 gttctttcac ttaaaccggt ggatgcggca gtcattccaa acgttcgtat aattgctgtt
    12841 ccaataccgc cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct
    12901 tgacgattgg ctggtgaagc aagagaaata agtgcatctt ttccagtgat ttgctgtttt
    12961 acaacgatag catcccccac cgcataaata tcaggaatat tcgtttgata acgttcgtct
    13021 actaggatac ccccgcgtat gcctaattcg attccagcgg tcactgctag ctggttatca
    13081 ggacgaacac caatggataa aataactaaa tcagcaggga tggtacgccc atcatccagt
    13141 cgcaatcgtt tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat cacttctact
    13201 ccttgatgag acaattcttt ttctataaaa gctgccattt cttcatccag tgttggaaga
    13261 atatgtggtg cttgttcgat caccatgact tctaaacctc ttctttttag gttttctgcc
    13321 atttccaatc cgatgaatcc tgcaccaacg atcacggcac gctttggctg tttttctaat
    13381 gcatgcatca ctttatcgat atctggtaca tttctaatag aaaaaacatt gtcggcttct
    13441 gttatccctg gaatcgatgg aacaactggt tttgcaccag gagataaaac caatgcatcg
    13501 tactgttcaa tgtgtttttg accctcatgt acgacttcta ccgttttgtt ttcgggaaag
    13561 atggctgtca cttcatgatt tggacgcaca tctaaacaaa aacgagcgga taatgattct
    13621 ggcgtttgga caagaagatt ttcccgttca ctgatttctc ccgaaagata ataaggcaag
    13681 ccgcaatttg caaaagaaac atacggtcct ttttcaaaaa caacgatttc tgcatcttcc
    13741 attaatcggc gaagccgtgt agcagcagac attccacctg ctacacctcc gacaataacg
    13801 attttcatct tatttccctc ctactgttcg atcaaaccat tgattcatgc caccacgtac
    13861 attcactaca tcataaccag atttctttaa ttctttagct gcttgtttac tcctcatccc
    13921 tgattgacaa atcacataga cggtcttttc tttgtctcca tcatatctgt ttatactttg
    13981 taatggaaca tttttagctc cttttatatg cccgccccgg tattctgcag gtgttctaac
    14041 atcaagtaac gctggatttt ccaaaagctt ttcctttaat tctgggactg ttatcgattt
    14101 tattgattga aatagcaaat tcattttctg cacctcattt acatttttat aaatacctca
    14161 tggggtatat taaacaggag ctttattaat atgtcaacca gaatgactct tcattttttt
    14221 cactactttt tatatttgca taaaataccg ataaggggta ttatctaatt ataaaaatag
    14281 aggagaatgt gttatggaga ttgataataa aaatatcctg aaccgtttga aacgtacaga
    14341 gggacaaatt cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat
    14401 cactcaatta agcgctgttc gttctagtat tgatcgcgta atgggcatga tcgttgcaga
    14461 taatctgaaa aattgctttg agaaacctga tagtaatcca gaagaacaag cggctaaact
    14521 agaacaagcg atcaagatga ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt
    14581 tccatattga tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa aacctgataa
    14641 aagcttctcc agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt accggttatc
    14701 tagactctgc caatgcagaa ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca
    14761 atgctatgcg tgctaatcat atttcagaaa gttctttacc tgaacaagta atcgttgtac
    14821 cagcaggtgt ccttgattta gttgaactgc aatcccaagg ttatgcttat atcaagcctt
    14881 agtcagtaga ggttgcgaca gaagtgatca gcttcaagaa atttcggctt atttttcgaa
    14941 gaagctactt ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta
    15001 ataagaacag ccaaacaaaa atagcttctc atatttttcg tttggttggg cttattaccg
    15061 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta
    15121 agtattagag aaaaaaacgg gaaaatagct cctcatattt tgccgttttt tgagctaata
    15181 ccgaaggtca gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt
    15241 cgagtattaa gaaaaatttt gtgaaaatgg atttctacat ttttgcataa atttggctta
    15301 ataccgagaa actggctttt gaataccgtt taaggaagag gttgtaaaat cagcggtttg
    15361 acctgagtat tagagaaaca aacggaaaaa tagctgctca tattttgccg ttttttgagc
    15421 taataccgaa ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctaccattta
    15481 actccgaatc acaagaaata atttcaaaaa caacttctcc tgtttttggt tcggttggct
    15541 tattatcgag aagtagctac tgggagtcta tttatcaaaa aaaggaatgc gacataggtc
    15601 acaccccttt tttattttct ttgaaacatc tttcggtact cgacagcata caagacaaaa
    15661 gattgtaaaa gaccgtaagt gatgacggca aaaatgactc tcacccacag cgttggtaat
    15721 tctgggaaaa gcagcaaaag gatcatgcca acatagatga cagcagtcgc tactttgcca
    15781 taccacttcg ctccacttaa tttgatattt ttagatagcc cgattaatcc agctacaaac
    15841 ataaagcctt cttttatcaa gaatagacca aacaacagcc aaagccacgg tcgataccat
    15901 gccatagaca aaatcagaaa aagttgtgtc aatttatctg caaacggatc aagcaccttc
    15961 cctaagtctg ttacctgatt atatctccga gcaatgaccc cgtctagata atcagacaaa
    16021 cctgagacaa caatgaccgc tcctgctata taaaatgact gtctcagcac aaaataaaga
    16081 taaatcggca gcaaaaaaat acggaatatc gataaaagat tcggaatcgt ttgtatttct
    16141 ttacgccagt ctgtgcttaa tttcatttta tcgccctacc tcctcagcac tcttttcttt
    16201 ttattttagc ttacacgggg aataaaacca atgatttcac tgtttttatt tttctttaca
    16261 gacactgata ttatgagcta atttgagata atcacttgct gttttcatcg tttactcttc
    16321 tttcttcctg tcgtttgcgc atttctttta ctagtacttt atgagctttt tttgatcctc
    16381 gctctaggtc acgctgccgt ttacgtgttt tataatcgat aaacagactg tctaaatcgt
    16441 aacctgtcgg gtacagatct tctgctgaac gcaccagacg aacacgctga cgaggcacgt
    16501 ggattatttt tttgtctaaa tatacatcaa tcgtatcttc acctatatct tcataaacta
    16561 acgcttcttt ttggtattca ttgacaaata tccgatcacc ttttgcaaag agctgacgtt
    16621 cttttttatt ctccttccta tttgtaactg ctgtcttagg aagaaattct tttttctgtg
    16681 tccgataggc tttttccgcc aagtaacgct ctgcttgatg gatcaatttc atcgacatat
    16741 ccattttatg agcaatccac aaagcttggc tttctcctgt ttcttccaac ccgcaactga
    16801 tatttaggac gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa atcttcatga
    16861 tctccggcga atttcttgat ttctccataa tgggtcgtgg caatgatcaa tgccccttgc
    16921 tcatacatag attccatgat tgcgatcgct agtgcagccc cttcatttgg ctctgttcca
    16981 ctgccaatct catctaaaag taccaacgta ttccttttta tctttttcaa aatagcagcg
    17041 atgttttgca tatgaccaga aaatgtactc aacgcatttt ctaaattttg gtggtcaccg
    17101 acatcgacga aaatctcatc aaagacagca agctctgtcc cttcttttgc cggaacttgc
    17161 ataccgaaca tcgccatcaa agtcaataaa ccgactgtct tcaacacgac tgttttgcct
    17221 ccggcatttg ctccggtgat aatcaagcct cgatagtctt taccgatttc aacatccaat
    17281 ggtaccgcat gctcaggtaa aaatggatgt ctgccttgct tgataatgat atgttccgat
    17341 ttattgattc taggtgtgct tccattgatt tctcggctaa attttgcccg agcaaagatg
    17401 atatctaaca ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata
    17461 gcaccagtca atgccgccag gacttgatat tcttcactga tttcttcagc ttttaacaac
    17521 tgatagtgtt cattcaattt agaaatcaca gttggttcaa taaaaaccgt cgttctttta
    17581 tttgattcat cgataatcgt tccatctatt ttatttttat agctggcttt gataggaatc
    17641 gtatagtatt cgcctttttg aacaatcatt gcttcttgga tcatttcttt gtttttcgga
    17701 tgacgcaaga atttcagtaa ttttgactgg atttcttttt cgattagttg taattgtttg
    17761 cgtactttac gcaaattacg tgacgcgtcg tcactgactt tattattttt tattttctgg
    17821 tcaatcaact cttctacatt tatcaaatca ggaagatgtt tgctataagc aaacaacaag
    17881 ggtgtctggt attgattctt atcaaaaaac tttgtgatca tccgactgct tctaagaaaa
    17941 tccgcatatt cgatcaaatc ggttggctgc agaatcaacc cttttttgac ttgttctgtc
    18001 agtgcatcga tcctcggcaa ccccataaat ggaacatgct gactgctttc taagatcaag
    18061 cgtgcttctc ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc
    18121 atatcgctga ttcgtttttt gctatagttt ccaattgctc ttgcttggat ttcctttttg
    18181 atccgatcaa attgtaattg actaatcatt tcgttgtcca tattctctcc ttttcttgaa
    18241 gagatacctg tcccaaaata aataagggcc tagagataaa taaaaggata gaacacagct
    18301 taaaaaagtc atgtcctatc cttattagaa tacaaagata ttgttttcct gacaggtacc
    18361 gtttcataag cacaccaaat aaactttgtt tattggctat tcgacttact gattagatac
    18421 cagctacact cacaaaacat accccattct tctaggtctt atttattttc cacttaacta
    18481 tagcaagaca cattttggtt gtcaaacttt agtatgtttt atagatttac accattaatg
    18541 aatagacacc taccgcgata attgctccga tcattggacc tgcgattggt acccatgaat
    18601 atgcccaatc tgaagttcct ttattagcta ttggcaatac cgcatgtgct aatcgaggac
    18661 caaagtcccg agcagggttg attgcatatc ctgttggccc tcctaatgaa agaccgatgg
    18721 ctaagatcaa tccacctaca gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa
    18781 aagctagcaa cccaagaact aatacaaatg taccaattaa ttctgtgatc acatttgctg
    18841 gataattccg tatagcagga cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg
    18901 ttgcattcca atgtggcaaa taagaaagcc aaacaagaat ggcccctgca aaagctccta
    18961 aaacttgcgc aatgatgaaa ggaacgacaa gattccaact aattgcccct gtcatcgcca
    19021 tagctagaga gactgcagga ttcaaatgag ctgggcccat gaatccagaa atatagacag
    19081 ccattgttac agcgagtccc caaccaaatg cgatgacaat ccagccagaa gcctgtgctt
    19141 tacttttgtt taaattcaca gccgcacaca caccatctcc aagcaaaact aggatcagtg
    19201 tcccaataaa ctcccccatg atttgagtca tatcactgtt catttatttc tcccccttta
    19261 gttcccgcag atctgattct gcaatcaatg cttctaaacg tttagtctgt tgcgccttct
    19321 cctcttctgt ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc ttgatctcat
    19381 caagctgatc tcgttcaaac aacagatgat ttgtacgacg aatgagataa tcgcctgggg
    19441 ccaaaaccat ttcttcttct aaaccataac gtaaccgagc tgactcagcg agactcaaac
    19501 caggataagg tgccatttct tttgctaatt caaagatacg tcgggcatta gtcccgtaaa
    19561 aatcagcgat ataagtagca tcttcttcct ctaaaccggc tgctactcct tcttttgcta
    19621 attctgtcac tgtttcttct aatttagttg gatcgaagtt tccacctgaa atctgatatt
    19681 ttttagagtc gatttctttc gtctctattc cgtattcttc ttttaataat tggcgtatca
    19741 atcgcaaagc tccttctgcc atcttacggt aatcggtgat tttcccacca gataaagtca
    19801 gcaaaccatc tggttctctt tctaaagaac tacctctgga aatcgtagaa ggtgcttttt
    19861 catcacggct gttttccaaa tggttcaaca cgtcttctac ttcagcacga gaaactttgt
    19921 tttccttata ttcacttact gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc
    19981 cattatctcc accattgtaa tcagaaccag aattaccaat caacagtgga cgaagtcctg
    20041 cccagcttgc ttcgatatca ttcaatgtga tatttgcttc tggatagcga tgattgatca
    20101 catccaatag ataatctaca tcttcttgtg tgactttcgg atccgtaaaa tctccttggt
    20161 aatccgtatc tgtcgtacca aagtaagtct tgttttctct tggaatagca aaaaccatcc
    20221 gcccatcttg ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat
    20281 ctacaactaa atggatccct ttagttggac gcattttagg agagactgga cgcgtaaaat
    20341 tcaagtttcg tactttatct acccaaggac cactcgtatt gatcactaat tttgctttga
    20401 tttcgatcac ttcatctgtc agcaggtcac gagctttgac gccaacgatt tgatcgccat
    20461 catataaaaa gccaaccgct ttcattttac ttactagata agccccatct tcggcagcct
    20521 ttttgatatt atcaatcact aaacgggcat cattgttgcg gaaatctaga tagacacccg
    20581 cgccttttag cccttctttt ttcaaaaacg gttctctttc caatacttct tctggtgtca
    20641 gcgtataatt ctcatatttg gttcctgtca catttgccag tttgtcataa aggtccattg
    20701 ctactttgac cgagaacata ttgaaggttg ttgctccttc gccctcatag attggcaaaa
    20761 gcattggatc tggttttggg atgtgtgggg caatactttg tacgactgca cgttcaccaa
    20821 ctgtgtcagc tactacttcc acatcaaatg tcttcagata acgaataccg ccatgaacaa
    20881 gtttggtcga gcgagaggaa gtcccttctg caaaatcctg catttcgatc aatcctgttt
    20941 tgattcctga tgctgctgac tggactgcca caccggcacc agtgattccg ccaccgatga
    21001 tcaacagatc caattcttct tgctgcattt tttgaatgct atcttgtctt gtcttgtttg
    21061 aaaacatatg gatttcctcc ttattcaaat gcttgggtag cttttactgc ttttttccaa
    21121 ccgttgtaaa gtttttctcg gcgctcttcc tccatttgaa cttcaaatag cttgccagct
    21181 tcataaaatt cgcggatctc gtctgtatct ttccaataac cgacagctaa tcctgctaaa
    21241 aatgctgccc ctagcgccgt tgtttccaag ttttctgctc gttggatcgg tacattcaag
    21301 atatctgttt ggaattgcat cagatattca ttgtttgctg caccgccatc tactttcaat
    21361 acaggaatct tgattccagt gtcatcttgc atcgtatcga tgatatctct cacttgataa
    21421 gcaatcgact gtaaagtagc tttaatgata tcttcttttg tcgtaccacg tgtcaagcca
    21481 aacatagccc ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac gaatgcagga
    21541 acaacataaa cctcatcttc gcttgtagag cgtttcgctg cgtcttctga atcgctagct
    21601 ttttgcaaca tttgaagtcc atctctcagc cattgaatcg aagaaccagc cacaaaaata
    21661 cttccttcta aagcatagta aacctttcca tttatgccgt aaccaattgt cgtcaataaa
    21721 ttatttttgg ataattgcgg tttttcaccg gtattcatga caataaatga acctgtacca
    21781 taagtattct tgaccatgcc tggttcgaat gccatttgtc cgaataatgc ggcttgctgg
    21841 tctcctgcca tccctgaaat tggtgcttcg cttccataaa aatgataacc ttgcgtataa
    21901 ccataaactt ctgagttaga agtgactttt ggtaacatca ctcgaggaat atttagtaaa
    21961 tctaaaatct cctgatccca atctagctga tggatattga acaacatcgt ccgactggca
    22021 ttcgagtaat cagtaacatg tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg
    22081 atcgttccaa acatcaattc gcctttttca gctcgttctt gcgcgccttc tacatgatca
    22141 agaatccagc gtatcttcgt tgctgagaaa taggcatcga tcactaaccc tgttttttcg
    22201 tggaaaaatt cttgatagcc ttcatttttt aagttatctg caattccagc tgactgtctg
    22261 gattgccaaa caactgcatg atagattgga cgtcctgtat ctttttccca tattactgtt
    22321 gtttcacgct ggttcgttat gccaatccca gcaatctgat ttggtttgat ccctgattcg
    22381 atgaacgctc cggcaataac tgactgtaca gaattccaga tttcatttgc atcatgttcc
    22441 acccaacttt cttgcgggaa atattgcgtg aactcttttt gagaactgcc gatgtgtctt
    22501 cctttcttgt caaagataat tgctcgtgag ctagttgtac cttggtcaat cgccatgata
    22561 tacgttgatt ctgtcattgt gattccctcc attatatgca aacgctttct ttaactactc
    22621 ttatcatact actgttttaa aaaaagaaac tgtcaaaatt ttattctttt tttctttttt
    22681 tgacagtttt ttattatgaa gctatttttt aatcaattct tttctttttt cagcgtctct
    22741 attcttcttt tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag ataatattca
    22801 cactgttttt tgtttctctg gcaaaatgtg atcaccaaat catattcttt accttcttca
    22861 taacttccaa tctcaatacc gccgattccc ctcagttctc tgatcagata ttgttgaaaa
    22921 gcaatacgat aaatcggcag actttctaaa tcgattccaa tcactactgg ttttgcaata
    22981 taaaaatcga tcatcaaaag aacattggca tactggatca tcaggtaact aagctcacta
    23041 tcttcagctc ttttcctcgg caattgctgc accgctgttt cctgtaattt cgccagtaac
    23101 tgtgttaagg aatgcccgag cattttctgc tgctgttcca aaagccgatc acgctcatag
    23161 acttcgatct ttccaacaaa gaaaaaccat tcattgttga tctgggaaat ttggtagcct
    23221 actgcctttt cttctttgat tttcaaacga cgaggacgat aatgccataa catcgtttcc
    23281 cgaatgtaag tatctaaaac agcggttggt aactttttgc tccgtgtcaa atcatactga
    23341 taaaatattt cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt
    23401 tcgtatacat tcagttctgc tgctgttcga ctaagatgca tcacgatgat tggatcgatt
    23461 gcttgataaa gccggtccga ctgatagagt aatttctttt ctttcaaagt tgcgtacgta
    23521 cttttttcat tcaatagtct ttttttcgta atcccgagcc agcaagcaat ctgtgcttct
    23581 gctgatgccg taaacgttgt ttctaataca cggtccaatc ctagaatgaa cggtcttttt
    23641 tctggtgtat tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg gaatagttca
    23701 taataaaaat atcgtatctg tagctcttct ccgtataatt gaccgttttt gatttgtaat
    23761 ccaaattcct ctaagagctg gttcaattct ttgatctttc gaaaaacaga agactcactg
    23821 atattaaacg ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa taaatagacg
    23881 agcatcttaa acttcaagct ttcctgcaat aaaaacaata gaattttttc caaacttaca
    23941 tcttcgtcca acacaagcag cgcctgatac tcatttcgct gtacttctac ttttttttcc
    24001 atcattcggc caatcaattc aatatcttcc aaatattgat caaacgaggc ttttgacagt
    24061 tggacatgct ctctcaaacc attgatagag gctgttcccc cttgcagaac aaggtttcgc
    24121 aaaatcagga tctgtaactg ctcttttctt tctaataatg cttcaatctt cataggcagt
    24181 attcgccacc tttgaccaca ccatatctgg cacacggtat tctggctgga aaaatagctc
    24241 atgttcttct aactgttcaa acgtccatcc ttcttctact gcaattgtca gttggtcaag
    24301 aaggtggaac atctcttttt tgctttggag ctgtgctcca agaatctttt tttcagaatc
    24361 aaatagaaat ttgagtttaa ctttgctttg atttattgct gtaagcttgt agtctacgta
    24421 tcctgtttca attttttggg gataaaaaat gctctcttct tctgtcaaac ctacgcttcc
    24481 taagaaatag ccaaaccact gattgccgac tggacgatag gttccctgat cctttgtctg
    24541 tgagccacta attgtcttgc tgacatttcg tgctgtacgg acagcgttat ttactagaga
    24601 aacatacatt ttttcatttg ttggacgaaa agttacttgg atcgcatcgc caatcgcata
    24661 aacgtctttt tgagacgttt gcaagtagtc atctacccag atcgttccat catcattcaa
    24721 tgttaattgt tcttcccaca tctgattgtc gggacgagaa tgattagcca gcaaaacaag
    24781 atcatagttc tcctttcttt tttccgtctc tacaatagcg gtttcatttt cggtcaaaga
    24841 cacaacttgt tcttctaaaa aaagtgaaag cccttgattt ttcatttcct ttactaacgg
    24901 ttctgtcatt tcaggatcta gataacgaaa aagaattgac tttcggcttt cgtaaagatg
    24961 gatttgtttt tttcttgatg ccaacccttc agctatttcg attcctgctt gtcctgcacc
    25021 aataattgca attttctttg cattcatgat cttttgctga agagccgtta cgttttgtat
    25081 ctttttaact gggtgaataa gcgttgattc tgcagaaata tttcgaaact tttgatttga
    25141 accagtcgct agaatcaaac gatcaaaatc caattttttt cctcctgcta aaacgacttt
    25201 tttttctttc aatcctatta ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt
    25261 cagttcttct ttcgtgatcc aagaaagttg tttttcactg gtaacctttc cttttaatat
    25321 caaattgaac ccgctgggaa tccatcctaa cttcgattgc ttttcaatca aatatatttc
    25381 acttgttggc atcaattggc gaagataaat agccgcatga atccctgcaa acgacccgcc
    25441 gacaattaca atgttcatct gcattcctct ttcttataag gtgttctact gctaacttct
    25501 caatcagaaa acacagtatt cataactcgc tttcaacctt atgatacata gcgatgcagc
    25561 agaatcaacc ctctccatca taccgtgttc taggtctgct tctcaataat gtactagtgg
    25621 acctaattga agagtatcat ttctgaagag atgtttcaaa aaatgtactc ttataaaaga
    25681 aaaaaactcg ccttttacgt acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg
    25741 agttttagcg tgtgtaatgt aatagttaga atagacacat catttatttt taattcttta
    25801 atgcttcaac aactgcttcg cctaattgat gagcagattg aggattttgc cctgtaagaa
    25861 gccgcccatc aatttgaacc acttctgaaa aagctgctcc agactgatag tttgctcctt
    25921 tttctttcaa agcatcttct gctaagaatg gtacttcatt cgttgtacca tttgcttcct
    25981 cttcttcatt actgaaccca gtcactgtac gtccagctaa aatagatttt ccagactcat
    26041 ctgtaactgg caacaatcct acaacaccgt gacaaacagc tgagaccagc ccttcatttc
    26101 gatagatttg tgaagcgact ttcccaagat ctattgcttc tggaaaatcc cacattgttc
    26161 cgtggccacc agcataataa accgctgcat aatcatttgc atccacatca tcaggacgta
    26221 aggtatttcc caaggcccga tttctgaaat cttcatcttc ataataggcc caatcgattt
    26281 catccatatt caaaacactt tgcggttcta aaggtacata tccgccttta ggactgacaa
    26341 aatctgcatc gatcccattt tttactaata catcataaaa atgagtcagt tcacttaacc
    26401 acaaacctgt aggacgctcg atcttttcgt acttttctac atttgttaat acgatcaata
    26461 ctttcatttt cccatcctct ttctataaaa attgcgttat tgataacgtg ctttcaagaa
    26521 attatataat gggcttggca aataaagctc tttatatgct ttttcaaaag gtttcgttac
    26581 aaattcatcc tcacggtttt ccaataattt ctctgcccag tcaggatcga cgatcatttg
    26641 ctgtccaatt gctgcaagtt ctgcatattt caatacctgc tctacatctt cgcgattgcg
    26701 gatcccaccg acaccaacta aagggatctt tccttgaatc gtgttatgga ccacttcaag
    26761 aatcgattta tcgttgtatt tatcagaacg agctattcga tcatacatat ttaatgaaac
    26821 atgcagataa tctagttttg actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa
    26881 acggatacct ggtgtttcat attcttctgg tgagaaacga taccctacga tgaatggttt
    26941 tttagcatat tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa taaaacgata
    27001 acgattttcc aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa
    27061 ttgttggatc aaatacgtat ttgctccatg caactcgatt ccatcaaatc ctgattcaat
    27121 cgctctacgt gtggctttgc caaatgcttg gattgtttca acgatttctt cttcactcat
    27181 tgctcgtggt gtttccgcat caggacgttt tgctgcaact gcacttgcag atacaggctg
    27241 ttctccagat aaagttgctc gacttgtcat tcgtccaccg tggaaaatct gaataatcgc
    27301 ttttgcgcct tctccttgga ttgctttcgc tagctcactt aatcgaggaa tcatttcatc
    27361 atgagcgatg ctgagttcac ctggccagcc ttttccgata tcttggacat ttgcagcacc
    27421 agtaataaca gcaccaagac ctttagaacg ttgtttataa taagtgatct catcttcggt
    27481 gatcactcca ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca atcggttctt
    27541 caattgaact cctgaaggta aagtaattga ttgatcaaat tgtgacattg tcattcctcc
    27601 cataattcgc tctgattaac ctatcgagaa tcatctatca ttaaaccact cgattagttg
    27661 tacatgcaac taatttgctt aaaaaatttt attttttttg taacttatct gttgcatcat
    27721 tcattgtctg ggctaattct ttacttgctt catttcccaa aactgctttg tatgactgat
    27781 gaagttcttc ccagctacca tcgacttcct tagcaaattg gcgtccttgt tctgtaagat
    27841 aaactaatga caagcgacct tcgctaactg ttttgacgta tcctttatat tctaattttt
    27901 caacaaaacg agcaatcgtt gatggggcgg aatagagcat ttgtgcaata tctttttgtg
    27961 gtattccatc tttttctttt aatatcaata aaataaaacc ataagttggt gccagacctg
    28021 tccttctaaa cgcttcatca gctaatttat tcaacatgcg atctagcttt ttcacgctaa
    28081 agaaaagaca ttcatctaac gctttttcaa ttggcttttc catttcatcc cctccattgg
    28141 cgtacatatc atagtatgat ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc
    28201 ctctgtctag atttgaaaaa aagaagtagc catcttcaaa gaatttcggc ttgttttcga
    28261 caaaactcct tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt
    28321 atgaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc
    28381 gaaaaactgg cttttgaaca ccgtttatat cagaggttgt gaaaaagccg tcctgcatca
    28441 agtaataaga acagccaaac aaaaatagtt cctcatattt tgtcgttttt tgagctaatg
    28501 ccgatggtca gctgattgta caccgtttat tgagaggtcg tgagcctgcc gttgaactcc
    28561 gagtcacaag gagcaatttc aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt
    28621 gatcgaggag ttggcaggag aatactgttt attcgtaaag aaggggctgt gacaagactt
    28681 ttgtcacagt cccttatttc cttttattat aggatttcgc catttgattg gatgacttgt
    28741 ctgtaccaat caaatgattt tttctttaac cgtttctttg tgccattccc ttcgtcatct
    28801 tgatcaacat agatatatcc ataacgtttt gacatttgtg aagtagaagc gctgacgata
    28861 tcaatgcagc cccacgacgt ataaccaaat aaatctactc catctttgat agcttccttc
    28921 atctgctcaa tatgctgctt caaatagtca atgcggtaat cgtcttgaac ggttccatct
    28981 tcttctactt tgtctatagc tccgagtccg ttttcagcta tgaacagtgg tttttgatac
    29041 ctatcatata attgattcaa agcaattctc aaacccttag gatcaatctg ccagccccat
    29101 tcacttgatg gaagatacgg atttttaatt cctccagtta ttaagtttcc ttggacccct
    29161 tcccaatttt ctggatgagc actgactgca ctagacatgt agtagctaaa agaaacaaaa
    29221 tcaacagggt aggctgctaa gatttcttca tcacctactt ctatttttaa gttgacacct
    29281 ttttcttcaa acattcgttg cgtatacgct ggataatatc ctcttgcttg aacatcacta
    29341 aagaacaaag aagttctttg ctgctcaatt gttttttgaa tatcatccgg atcacaagaa
    29401 tacggaacaa gaagctgata gctgatcata caaccgactt gtgaaccttt gataatttca
    29461 tgacaagctt ttgtagctaa agcacttgct ataaactgat ggtgtgcacc ttggtatttc
    29521 gcttgttcca aatggggatg gttttcttca attactccta cgctaacaaa agggtgatgt
    29581 tttacacagt tgatctcatt gaaagtcatc caatatttta ctttattttt atagcgtttg
    29641 aatactgttt cagcgaaacg aacgtaaaac tgaataagct ttcgattagg ccatccacca
    29701 tattctgtaa ccaaatataa cggcatttca taatgggaaa gagaaacgac tggctcaatc
    29761 ccatatttta gacattcgtc aaacacttga tcataaaaaa ataatcccgc ttcattgggt
    29821 gtttcttcat caccatgagg aaaaattctt gtccatgcga tagacatccg atagcattta
    29881 aaacccattt cagcaaataa acggatgtct tctttaaagt gatgatagaa atcatttccg
    29941 tgtcttttag gataataata cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc
    30001 gtcatcatat gccattgatc tgtccatttt tctttaggta aatcgctgtc gaatgtatac
    30061 acatcagaca cagataatcc tttttcatct acatcccatg caccttcaca ttgattggcc
    30121 gctgtcgctc cgccccataa aaatccttct ggaaattttt tcatatcgat ccccctaaac
    30181 ttgctagttt tcttgtttga ttatttttct tatgactagt aatgatattt ttgaatctac
    30241 tactatttgt aacaataacc attgtggtcg gatcatatcc ctctttgatt aattcttgca
    30301 aatctacttc catcaataaa tcgcctttac gtactttttt tccatcagat actgcacttt
    30361 gaaagtattt gccatttaaa ttcactgtat caataccgat atggatcagt atctctgctc
    30421 cttcttcact gactacccca attgcatgtt tcgttggaaa aactgttgaa acttccccgt
    30481 ttacagaact gtagattttt ccgtccgttg gaatgatcgc aaacccttct cctaaagatc
    30541 cactagcaaa tgttggatcg tttacttcag ataaatcaat tatttcacct gctgcaggag
    30601 tacttaacac tatatccttt gaggattctt cttcgatttc atccttgtag ccgaaaagaa
    30661 aagttagcat acaggaactg atcatcgcta agaaataact agcaactagc caaacaaatc
    30721 cttcaccaat ataaatcggg aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg
    30781 ctcctgcggc accagctact ccaccaaata tagctccaca tatacaagca atgtaaaatg
    30841 gtcgtttata tttaagagtc acaccataaa cagcaggttc agtgattcca aataatgctg
    30901 tgataaatgc agataaagat aaacttttta ctttcttatt tttcgttttc aaaaataccc
    30961 caagtactgc tcctgcttgc gcagctacgg cagtcataca tgctggacct agcgtatcac
    31021 gaccgtagaa agaaagattg ttggtcatta ctggtacaag tccccaatgt aacccaaaaa
    31081 tcactaacac ttgccaaaat ccgccgataa cagcaccaga taaaacaggg ctaaagttat
    31141 aaacagctaa atatccagct gcaatcagct gacttgcata atctgcaatt ggacctacaa
    31201 ctaataaaat aattgggacc atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa
    31261 tactttcgtg caagcttttg cgaatcttag gctcaatata agaaagaacc catacagcta
    31321 aaattgctgg aataaccgta cttgtatacc ttgctagaat cacgggcatc cctagaaatc
    31381 gcaaggttat gctatcacta tatgcactga caatcgtagg ataaacgaga gctgctgcaa
    31441 taaccatagc gatgaaacga tctgtattga acttttttgc agcagtatat gccaaaaata
    31501 taggcaagaa atagaaaaat gcatcagctg ctgcattcaa aatgatataa gttcctgatt
    31561 cagtagttaa taaattcaaa ctcgtacata aggcgagtac tcctttgatc aaaccacttc
    31621 ctgccaagac gcctaaagtc ggggcaaaaa cagcggcgat catgtctata aaagagttta
    31681 aaatgccttt tttctcagtt cctctctttc cactagatgc atcatcttct agtttcattt
    31741 gtttggataa tacgtcatac acttcattta ctgcatttcc tactacgact tgaaacattc
    31801 ctccagcttc gactacagtg atcacacctt ctagttgctc taccttctcc ttatctgcct
    31861 tcttattatc ttttagggta aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat
    31921 tttcttcgcc tccgacaaat cggacaatat ctttcgctaa ttgtgaatag tccattttta
    31981 cttccccctt gttaattaat gccgtagcaa tccttcttac aagataagta tagttcttag
    32041 aaaacggttt ctccattcaa ttagtacccc ttctaatagc gaacgaaact gtacaatagt
    32101 gtgtatggtt tacttagccg agcaaatcac tcactgcttg aaagaaaaca agtatatcgt
    32161 ttctcttata gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa
    32221 gtggtatctc aagctgatac tctgtgattt ttgtttctac ttggcttttg ataagacagc
    32281 acaacaaagt ctcggcattt aacggaagca taactaggtc agaactagga gagattagtt
    32341 gctgaatgga atctatcttt ttatggattt ttatttgatt ttcttctttg tagtaatgat
    32401 caagcagttt tgttttcttt ttttcttcaa ctatggtcag cttttcacgc aaaatctgat
    32461 aaatctccca agattcaaac tcatctagaa cactaggtag ataaatcaaa tcatcccact
    32521 caaaaataag ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt
    32581 gatttttgaa aagatacact ggttctatcc ggcattcttt gatccacatg ttcatacttt
    32641 gttgaatctg tttttttagt gtattcaaaa tactaattgg ctggttacta acaatcagta
    32701 tctccccttg ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca aaataagcgc
    32761 tttcagctaa attgggacgt aattgaaaat gtattgggaa gaaacgccga attaaataaa
    32821 tttctaaagg ataacgggag atgatctttt gtacaaaatg attcatggta ttatgtccgg
    32881 catccatcct cgttttcatt tgtttgatat gggttagcaa aaattctgtt tcatggaaca
    32941 ataacgaaac aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct cttagattct
    33001 tctctatttc tttgtttttt tcatcattaa tcatcaaata atttaactct aatagtcgat
    33061 tttcaattgc ttgtttttct tgattggaaa aagaataggg tagttctgat gataactttt
    33121 ttgtgagggt ctctaccatc ggaataaata atgtatacct gtttgttttt tctaaaagtt
    33181 cttctcttcg aagaatggtc aaaacgagat accgagaaaa tcgtatgaag tcctctcctg
    33241 aaacaatata attatatgat ataagaacct cttttaatgt cttttggaca gtcggcatga
    33301 tattggaaaa aattgtactg atttgttgag gaaaccttgc gtgttggatc accgtttctg
    33361 ttccaacaag agataagaaa attctttttg aattttcttc agcaatgatt tttagtcctt
    33421 ttgtcgacga aacttccagt tctaatttag gattgcgctc gatccaacgc aaaatcgttt
    33481 ttatttgtaa tgatacggct gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac
    33541 tgacataccc tttttgaaac aataagtacg tcacaatagt tgaagctcgg aaatccatgc
    33601 tggaatagat tttatgtttc gtcacctctt gtatgatttg ctgtttagtt tcagattcaa
    33661 ttgattctaa aaaataacct gaacgtttat ttgactggat aatgattttc ttctcgtaga
    33721 tagcaagcat atttttgatt tcagtttgga tcgttttctt gttggtacct aaagcttcag
    33781 ctaattcttc gcttgttatc caatgattct tatttcttaa tactacaaga atttggagtt
    33841 gtcgtttatt taagtccatt tgcttacacc tcctctttgt tataaacata caggaaaaac
    33901 ctctcaaatg acaatagtca cttgcagagg tttaaaaatc atttgaagaa agatctatat
    33961 tcaaggtttt taccattaga ctggataatt tgtttataat attcaaaact atcttttttc
    34021 aatctcctaa agcttccgct cccatcattt tgtctatcca cataaataaa gccatatcgc
    34081 ttcctcattt cccctgttcc tgccgatacg atatcgattg gtccccacca taagtaacca
    34141 atgatttggc aaccatcttt tattgcctca gcaatttcct gaagatgtat ttttagatac
    34201 tcacaacgtt ttggatcgtg gattcgattc aattcatctg gtgcttcatc tagtccaatg
    34261 ccattttcaa ctatgaataa tggtaattca taacggtctg tcaattcatt catgacataa
    34321 cgaaacccta gaggatcaat tggccaactc caaggttcgg gagatttttc cttcaaaaag
    34381 ggattatcga aaccatttgc tccgccagta tctacgcgca tttctgcttc ttttttatag
    34441 acagcgctac gataataact aaatgcaata taatctgccg tgttagcttg caagcactgt
    34501 tcgtctccat ctaacattac tggtgcacag tcttcttcct tccatgtcct gtaaatataa
    34561 gctggataat gccctcggca catgacatcg gagaaaaacc atgaattgcg tttaaattgc
    34621 agtgaaccaa atacattctc tggatcacag tcatatgcat aagtcgctaa tgcagaaaag
    34681 ctacacataa tacccatctt agcatttgga tcgattttct ttaataattg agtagcacgg
    34741 gcgttcgcta gaaacatatg atgacaggct tgataaatca ctttttttgt tagtgtagca
    34801 atgggttcaa gtttatctct agcagggaat gtaaccaatc cagcagctgc ataaggcata
    34861 cgaaaagcat tattgatctc attaaaggtc atccaatatt tcactttccc ttggtagcgt
    34921 tcaaagacgg tctgcgcata aaattcaaaa aagtcgatta attttcgatt ttgccacccc
    34981 ccatactctc ctaccaatgc taagggggtt tcataatgac taagagtaat tactggctcg
    35041 atatcatatc gatttaatgt attgattagt ctgtcataaa atacgagacc agcctcattt
    35101 ggcttctgtt cgtctcctct tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct
    35161 tttaaaccta actccttcag taaacgaata tcctcttcaa atcgatgata aaaatcaatc
    35221 gcttcatgag aaagataatg gagtgattcg tctaaagcaa tctcccaaat ctttttttct
    35281 tcattccact gaatactagg atgctggttc atgatcccga taagcgtatc tgtcacatta
    35341 ggtaatttgc cgtcttccaa ccatgcacct tcacattgat tagcagctac tgctccgccc
    35401 cataaaaaat tttctggaaa tatttcctga tccataacta tctctcctcg ctatcaacaa
    35461 tttcttactt gaagtatagc tcgattcatc aaaacaagat attctaacta tttcggcaga
    35521 agagaagaaa aaaattgaat gctactcttc atttttaata tttatatata acaaatacat
    35581 aagacgatcc ccgaaagaaa acactcgtta accatctaag caaacgattt attttacttc
    35641 caacacaaat gcttcataag gtcgtaaatc aactttgccg tctggtaact gtttcagagg
    35701 ataatttgac acaataaccc ttaaagcttc acgtggaata gtgaacgtct gcttttcact
    35761 ggacatatta caaataacga gccattgctg gtcttcatat atgcgagaat aggcaaagac
    35821 atgctcaggc gtcttttcca ctaattcata gtctccccag acgatgatgt catgttttct
    35881 acgcaaatcg atcaatttct tgtacatata aaaaacagaa tcttgatctg ctaacgcttc
    35941 ttctgcattg atttctttat aatttggatt gactcgaagc catggaattc cagtagtaaa
    36001 cccagcatgt tcacgggcgt tccattgcat aggcgtccta gcattgtctc ttcctttcgc
    36061 cttgattgat tccaagattt cctcttttgt aaatccagaa gaaatccttt cgttatacat
    36121 attgattgtc tcaatatctt ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc
    36181 taactcttct ccttgataga tataaggggt gcctttcatc atatggagca agatagcaaa
    36241 catcttggca ctttcttttc gatacacatc gtcatttccc caacgagaaa caattcgcgg
    36301 aagatcgtga ttattccaaa ataaactatt ccatccttca tcacctaaag atgtttgcca
    36361 tttggataat acttttttca gttcagagat actcaagggt ttcaaatccc atttgtcttt
    36421 gccttcttgc tgatctaatc ctatgtgttc gaattggaag accatggata attcatttct
    36481 tttaggattg gaataaagtt tagcgatttc ggtagttgct ccccatgttt cgccaacagt
    36541 catcagattt ttgtcaccaa aagttgcttg attcatttcc tgaagatact catgcagttt
    36601 aggtccatta cttgtgattt ctttatccgg ttctttgcca atcatatcga tcacgtccat
    36661 acgaaagccc ccaatacctt tatcgatcca gaaattcatc atttgataaa tctcttggcg
    36721 aacctgttta tttttccaat tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata
    36781 ttgccccgtt ttttcatcca actgccaagc agaaccactg aatattgagc gcaatctatt
    36841 aggctctttt ccattgaccg gatcacgcca aatataatag tcccgataag gattttcttt
    36901 atttttacgg gcctcaatga accacggatg ttcatcagaa gtatgattca ccaccagatc
    36961 catgatgata cggatatttc ttttctttgc ttcttcaatc agttcttcca tttcttccat
    37021 cgtaccgaat tctgccatga ttgcttgata attactgata tcatacccat tgtcatcatt
    37081 cggagactcg tatactggag aaagccaaat tgcatcaata cctaaatacg ccagataatc
    37141 caacctggaa ataatccccc gcaaatctcc aataccatct tgatttgaat cttgaaaact
    37201 tcttggatag atttgataaa caactgaatg gtgccaccat ttttgttcca tgattttcct
    37261 cctataaatg gatgtttcta ttttttgctt aatcatttat tttagtctct ctcactaaaa
    37321 attgtaacgc tttcaaatag ctattgcacc ttgttatcgg taacagaata aaacaaaaaa
    37381 acagctgagg aaattccctc aactgttttt tgaaaggatt gatttatgca gggaagaatg
    37441 ctgatttcac aacttcttta taaacggtgt tcactcagct gtcccttggt attagctcaa
    37501 aaaacggcaa aatataagaa actatttttt cgtttttttc tctaatactc aggccagaac
    37561 gctgatttca caacctcttt atttcacatt caatatccac atgaatccca caaatacaaa
    37621 gaacagacca tacatgattg gagatacttc ttttcctctt ttagctgcta acatggtaat
    37681 tggatagaag ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc
    37741 gatcaagatc aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa
    37801 agattgtgcc atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac
    37861 aactgatagt aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc
    37921 tgtgatacct gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac
    37981 aggagaagta ccaaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt
    38041 ccctacacga ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa
    38101 agttcctgct gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg
    38161 aagagaattg atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg
    38221 tgctgcggca acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt
    38281 tgtagctgcc atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt
    38341 tacgactaat ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac
    38401 taatgtagat tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcgataaa
    38461 tagaccgata ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat
    38521 ttcccgcagc ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc
    38581 taatgctgtt tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt
    38641 gatcccaaga gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaacaca
    38701 gccgatcgca ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc
    38761 acctaataca gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc
    38821 tgccagtacc tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat
    38881 tttttccttc atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt
    38941 tcgatttatt aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa
    39001 aaaattcgga ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa
    39061 gttcatgaac attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc
    39121 attctagtct caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt
    39181 tgatgaattc ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga
    39241 ccatttttcc ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct
    39301 tctcactatg ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca
    39361 ctgtttttcc tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg
    39421 ccttcagggt cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt
    39481 tcttccatgt tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt
    39541 ggtcctaata cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct
    39601 aattcttttg agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca
    39661 gaaatttctg caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca
    39721 taaagcatca cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt
    39781 tcctgtctat aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact
    39841 attcaagaaa aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca
    39901 aaaaaaaaac ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct
    39961 gattcatctt tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt
    40021 tccttcaacg cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca
    40081 atgaccagca ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag
    40141 ttgagacagc gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc
    40201 ggagcatcgg aatctgcacc taaaggcagg aatgattcgc caccttgggc ttttcctaaa
    40261 tgcagatttc ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag
    40321 gaaatactat tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt
    40381 gcacgtttca gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta
    40441 aatgcctgac gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa
    40501 tttcttgctt cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt
    40561 gttacgtggt tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact
    40621 tcccagaaat tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata
    40681 attgcttgat gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc
    40741 tgtatttgat aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa
    40801 caccgagcca aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta
    40861 aaaatgtacc gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta
    40921 aaaaatgatc agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg
    40981 caggctcttg atcgatactc actaggaaaa aatcggtttc tttatctttt agctcttctg
    41041 taagtttttc tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtgaattat
    41101 cttttccaaa tgtatctgta aatgtttcga tactaatttg ttgtagtaag tctacatctg
    41161 aaggcgtcat ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa tagatccttt
    41221 tattgccttt tttgacaaaa ttccaatctt cgctaacatt cttgcatact ttatttaaaa
    41281 gatattccaa ttgttttgct tcttcgtcag aaagaccatt taatgcgact gcattggagt
    41341 agttgttttc tcgaatgatg aaaggataga tttctgctcc ttttttagtc ggatagagtt
    41401 tcttgatttt tttattttct ttatcttcca aacgttcgat catcccattg gattctaatt
    41461 tcttgattgc tcgggcagtc gttgtacgat ctactttgat catttctgct agtttttctt
    41521 ggatgattcc aggattttcg caaatacgta ctaaataaag atactgtcct cttgttaaat
    41581 cgacttcttt gaattcgata ttgctaatag aatctagcgc tcgggcaatc attccgatgt
    41641 ctcgaaggat ttcagccatt tcatcccacc tttattttgt ttgtaagact agttcacctc
    41701 tttttgttgc aaatgcaact attttcaata aaaaaatcaa gttctttttt aataactagt
    41761 aaagtatatt cttttcttct tttacgaaaa agctcaatca caatagatct ggtttttgtt
    41821 ttctaaaata agtaactgtt tcttcgttgt tcgcttttct gatagctttg gcaactcttc
    41881 tatactaaaa aatcccatgt tggaagtttc attattctca ataaacttgg cctcttcacc
    41941 gtggatcgca caagcaaaaa caagtttata atactgaaaa agctgtggaa tatctttttg
    42001 tttattcgta tcaaaaactg ctcgcaactg agtagtttct actgttaatc ctgtttcttc
    42061 atagacttct ttacgcacat tttcttcagg agacaatcct atttcagcaa atccaccagg
    42121 aagtgaccat tctttcgtcc gtttatcttc gattagtagg accttgccat cttttttgat
    42181 ataggcacga acatctactt ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt
    42241 tatttcttcg aaagtttctt ttcctatttc actgatcagt tccaatgaaa tcgtccggat
    42301 ctcctcaaaa cgctctttat caaatacatc ttttccataa tatagaccag cttgagaaag
    42361 tgcgagtagt cttttgtatt gagccaactt cttgttcatt ctcttccctt cttttttagc
    42421 aaatgtatga ctatgtaatc aagtatagaa gactggttta attacggcaa ataaatataa
    42481 tgaaatctgc aataaataaa agaatgttaa aaaactcgag aacaaaagac attcgacttc
    42541 tctggggctg tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa
    42601 acgaaaaata tgacgagcta ttcttgtctg gctgttctta ttaattgagg caggacagct
    42661 ttttcacaac ctccccacta aacggtgttc tcctaccaac tcctcggtca caagtcacaa
    42721 tattcatcaa acatgagaag ctgttttttt gaaattgctc cttgtgactc ggagctgaac
    42781 gacaggctca cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga tattaagcca
    42841 tttctttgtt acttattgtc attgatttct ttttctaatt tatccaatag tggagtgtaa
    42901 tccacttgag tatagtctgt tacatcaaat tcatatagtg ttcctaactg gaatttgtca
    42961 tattttcggt cggcgatgat ctttttgaat gcttctttcg taatatgatt ggtccctttc
    43021 gttcgatctt ccaatacgtc accatagtga taatgatcca tgatatagct caaatgacga
    43081 tctgattcac gatcacgttt gtaccgacgt tgccataaca cttcaaagtc tgctgttagg
    43141 cgaatcgtga tcacctgata tccgtatcta tctgctaatt cttggagtcg tgctttttgc
    43201 ttatcactaa aaggatactc ggacacaatc actttctttc ctgcttccat atacaaatca
    43261 agtgctttgt aatagaaagg ataaactttc gtcctttctt gttccgcccg ttcttccaaa
    43321 ttgttaaacc cgatggattc tgcatagtat tccttgattt catcgagcgt gatggcatac
    43381 atatctggaa atcgttcccg tagtttattc ataagatatg tttttcctgt accaggacta
    43441 ccagccagaa gaatgaaata ctttttcatc tcatttattt cctaagttaa ttggactttc
    43501 caagttcata aacagtccaa tcttatgctt gacaatttct tttactgctt catttgctgg
    43561 aatcacatta tggcgaagag acttgttgat ttctgtttca ctaaaggttt ctttagctac
    43621 tttcgtccag ttgactaaaa gctccgttcc aacattgaat ttgcggatcc cgttattgat
    43681 caattcagga taatcttctt ctttcacacc tgttcctcca tggataacta gggggacatc
    43741 aactactgca tcgatttctt tcagcaaggg aatgtttact tctgtttttg atttgaattg
    43801 tccatgattc gtaccgatag caatagctaa tgcatccacg cctgtagctt ctacaaattt
    43861 cactgcgtct tccggtcttg tatatacctt gtcattttca gcgacatgga ttccttcttc
    43921 tgtaccgccg attgttccaa gttctccttc tacggaaaca ccgtgcgcat gggcatattc
    43981 tactactgct tttgtcttca ggatattttc tttgaaaggc agatgggacc catcatacat
    44041 gacagaagta tatccgctgt cgattgcttc tttgatatca tcaaagtctc gggcgtggtc
    44101 taagtgcaag actgcatcaa ccatttcttc ttctgcaata gctgtcatta cattgactaa
    44161 cacatgcatg ccgatatatt tagcggtatc gacacttgtt tgaataatga tcggcgaacc
    44221 catttctttt gctgcgccta gcatagcagg aagcatttct aggttatgcg tattaaaagc
    44281 tccaactgta aaattcaact cttctgctgt ttttgttact tctttcaatg ttgtatacat
    44341 cttttattgc caccttatct ttttatttgt ttcctttgat cgttacttgt tttgcaattt
    44401 gtcgcatctc atccattttg cccatgtagt aatcgatccc ttgatcatca ccagcacgag
    44461 cagcatctgc acgcttctgg ttatagaggc tcatcagttt tttataccca tctccgatcg
    44521 tctgtttatt ttccaaactc ttttctaaat attggatggc ttgatcgttc acatacaatt
    44581 cagaaaattt aagtccaact tgttcataaa gtttcgctaa tttatgctta tcttgggtag
    44641 tggaaatctc ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc gcttctggcg
    44701 atagggcttt ttcttctttt gccagaactt ttttcggttc tttttctttt ttcttaaaaa
    44761 atccaaacat cattttccct actttcaatt aaaatgcttt caaaactggg cttaagatcc
    44821 aggcatataa gagtgctgcg gctactgtat ctacatcggt tgctgtagca ttgttgaatc
    44881 ccatcgaatt aaagatcgtt actagcaaag cgggcaataa tgtaataaag aaaccatgag
    44941 caataccgcc aatgatcgct cctcggcgtc cgccaactgc gttgccaaat atccctgctg
    45001 tccctccggc aaagaaattg gttagcatac caggtaaaat catcgctaag ccgaacatgg
    45061 gcaaagtaaa catggcaata atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag
    45121 catttgggct ataaggaaag aaaactggac aatctaaggc agggatcgca tcaggaacta
    45181 atttcatagc tatccctcga aatgccggca cgatttcacc taacagtaga cgtacaccag
    45241 ctagcaatac atacacaccc actacgaatt ggatggcttg taaaaaggca aacatgatat
    45301 agttttgatc tcctgataat tcagaagcga atggttcgcc tgcaaataaa acagtgatga
    45361 tatatagcgg gaccatcacg accatgacag acaaataagt atcttgcaga aattcgaaat
    45421 gtgtcggcaa cttgatatct tcgatcgact ttttattttc gcctttctca ccaaaaagct
    45481 tagcaacacc ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa gcaatatcat
    45541 ttgatccagt gattttacga atgatcggtt gcgctactgc cggcatagca atggcgaaac
    45601 aagctccgat aaaaccaccg accaagatca atacgatccc tcgcagaccg gcaaaatagc
    45661 caaagactgt cgtcatcgtt gccatccata aaattgcttg accagttaaa aaaatgtact
    45721 tccattttgt gaaacgagcc aaaataatat tgaatacaaa aatggctaag aacgtcagtg
    45781 caatatctct acctaaaccc aaatcattca ttgcttgacc attgatagat tcaatagaag
    45841 ggatgatccc ttgcatatga aatccttcag taaagatttt cccgaaataa gttaaacttc
    45901 caacgatgat gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc
    45961 cagagatgac ttgtcctgct gatttcttct gcagtagtag acccaacata gcaatcaatg
    46021 caatggtgat cgatgcctga gtcaaaatat tttcgatgat aaaattgata acgcccatgt
    46081 tttgatgtgc accccaaaag ttagactaga aatctaatta aaaggggtgc tttttttgcg
    46141 gaaatataca tttgttttta agaaaaaagt agtttcagac tatttaaata acgaaggcgg
    46201 ctacaaatat cttgcacata aatatcaaat aaatcgtaca ctggttagac attgggtaag
    46261 gatttataac tatcatggtt gggaaggctt ggttggaggt ggcaaaagct acactacaaa
    46321 atttaaactt gatgttatag aatatatgga aacaaatggt ctttctatcc aagaaactgc
    46381 taaaaaattt aatatcggtt caaatagaac tctaagtaaa tggatagagc aatatgaaga
    46441 aggcggtgct tcttcacttg agagccaaaa aaggggcaga aaaattagta tgaattccaa
    46501 gctaaacatt cctaaaaaac ttaaagatga gtctcttgaa gaagaagtta ttcgtttaag
    46561 agcag
//
LOCUS       DAZKOU010000012        45930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000012 DAZKOU010000000
VERSION     DAZKOU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.012"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..279)
                     /locus_tag="ACG827_000627"
                     /pseudo
     CDS             complement(1..279)
                     /locus_tag="ACG827_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(533..2168)
                     /locus_tag="ACG827_000628"
                     /pseudo
     CDS             complement(533..2168)
                     /locus_tag="ACG827_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2491..2970)
                     /gene="rlmH"
                     /locus_tag="ACG827_000629"
     CDS             complement(2491..2970)
                     /gene="rlmH"
                     /locus_tag="ACG827_000629"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0482792.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3398..4687
                     /locus_tag="ACG827_000630"
     CDS             3398..4687
                     /locus_tag="ACG827_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0482793.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4726..5331)
                     /gene="ytpR"
                     /locus_tag="ACG827_000631"
     CDS             complement(4726..5331)
                     /gene="ytpR"
                     /locus_tag="ACG827_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0482794.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5536..6000)
                     /locus_tag="ACG827_000632"
     CDS             complement(5536..6000)
                     /locus_tag="ACG827_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0482795.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(6022..6342)
                     /locus_tag="ACG827_000633"
     CDS             complement(6022..6342)
                     /locus_tag="ACG827_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0482796.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6486..7565)
                     /gene="pepA"
                     /locus_tag="ACG827_000634"
     CDS             complement(6486..7565)
                     /gene="pepA"
                     /locus_tag="ACG827_000634"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0482797.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7765..8115
                     /locus_tag="ACG827_000635"
     CDS             7765..8115
                     /locus_tag="ACG827_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0482798.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8338..9285
                     /locus_tag="ACG827_000636"
     CDS             8338..9285
                     /locus_tag="ACG827_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0482799.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9342..10760)
                     /gene="pepV"
                     /locus_tag="ACG827_000637"
     CDS             complement(9342..10760)
                     /gene="pepV"
                     /locus_tag="ACG827_000637"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0482800.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10909..11754
                     /locus_tag="ACG827_000638"
     CDS             10909..11754
                     /locus_tag="ACG827_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0482801.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11758..12198
                     /locus_tag="ACG827_000639"
     CDS             11758..12198
                     /locus_tag="ACG827_000639"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0482802.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12248..12442
                     /locus_tag="ACG827_000640"
     CDS             12248..12442
                     /locus_tag="ACG827_000640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482803.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12578..13264
                     /locus_tag="ACG827_000641"
     CDS             12578..13264
                     /locus_tag="ACG827_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0482804.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13672..14361)
                     /locus_tag="ACG827_000642"
     CDS             complement(13672..14361)
                     /locus_tag="ACG827_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482805.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14336..16465)
                     /locus_tag="ACG827_000643"
     CDS             complement(14336..16465)
                     /locus_tag="ACG827_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0482806.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16513..17850)
                     /locus_tag="ACG827_000644"
     CDS             complement(16513..17850)
                     /locus_tag="ACG827_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0482807.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(18102..18461)
                     /gene="rplT"
                     /locus_tag="ACG827_000645"
     CDS             complement(18102..18461)
                     /gene="rplT"
                     /locus_tag="ACG827_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0482808.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18514..18714)
                     /gene="rpmI"
                     /locus_tag="ACG827_000646"
     CDS             complement(18514..18714)
                     /gene="rpmI"
                     /locus_tag="ACG827_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0482809.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18786..19310)
                     /gene="infC"
                     /locus_tag="ACG827_000647"
     CDS             complement(18786..19310)
                     /gene="infC"
                     /locus_tag="ACG827_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0482810.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(19354..19479)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19630..20262
                     /locus_tag="ACG827_000648"
     CDS             19630..20262
                     /locus_tag="ACG827_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0482811.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20264..21169)
                     /locus_tag="ACG827_000649"
     CDS             complement(20264..21169)
                     /locus_tag="ACG827_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0482812.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21254..21913)
                     /locus_tag="ACG827_000650"
     CDS             complement(21254..21913)
                     /locus_tag="ACG827_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0482813.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            22229..23173
                     /gene="mvk"
                     /locus_tag="ACG827_000651"
     CDS             22229..23173
                     /gene="mvk"
                     /locus_tag="ACG827_000651"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0482814.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23166..24143
                     /gene="mvaD"
                     /locus_tag="ACG827_000652"
     CDS             23166..24143
                     /gene="mvaD"
                     /locus_tag="ACG827_000652"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0482815.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            24156..25241
                     /locus_tag="ACG827_000653"
     CDS             24156..25241
                     /locus_tag="ACG827_000653"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0482816.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25238..26302
                     /gene="fni"
                     /locus_tag="ACG827_000654"
     CDS             25238..26302
                     /gene="fni"
                     /locus_tag="ACG827_000654"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0482817.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26551..27354)
                     /locus_tag="ACG827_000655"
     CDS             complement(26551..27354)
                     /locus_tag="ACG827_000655"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0482818.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(27374..28216)
                     /gene="kduI"
                     /locus_tag="ACG827_000656"
     CDS             complement(27374..28216)
                     /gene="kduI"
                     /locus_tag="ACG827_000656"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0482819.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28232..29239)
                     /locus_tag="ACG827_000657"
     CDS             complement(28232..29239)
                     /locus_tag="ACG827_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0482820.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29246..29890)
                     /locus_tag="ACG827_000658"
     CDS             complement(29246..29890)
                     /locus_tag="ACG827_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0482821.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29990..30748)
                     /locus_tag="ACG827_000659"
     CDS             complement(29990..30748)
                     /locus_tag="ACG827_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0482822.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30875..32026)
                     /gene="fucO"
                     /locus_tag="ACG827_000660"
     CDS             complement(30875..32026)
                     /gene="fucO"
                     /locus_tag="ACG827_000660"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0482823.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(32108..32419)
                     /gene="rhaM"
                     /locus_tag="ACG827_000661"
     CDS             complement(32108..32419)
                     /gene="rhaM"
                     /locus_tag="ACG827_000661"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0482824.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(32421..33266)
                     /gene="rhaD"
                     /locus_tag="ACG827_000662"
     CDS             complement(32421..33266)
                     /gene="rhaD"
                     /locus_tag="ACG827_000662"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0482825.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(33280..34536)
                     /gene="rhaA"
                     /locus_tag="ACG827_000663"
     CDS             complement(33280..34536)
                     /gene="rhaA"
                     /locus_tag="ACG827_000663"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0482826.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34533..35993)
                     /gene="rhaB"
                     /locus_tag="ACG827_000664"
     CDS             complement(34533..35993)
                     /gene="rhaB"
                     /locus_tag="ACG827_000664"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0482827.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            36146..37015
                     /locus_tag="ACG827_000665"
     CDS             36146..37015
                     /locus_tag="ACG827_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0482828.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(37141..38040)
                     /locus_tag="ACG827_000666"
     CDS             complement(37141..38040)
                     /locus_tag="ACG827_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0482829.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(38205..39029)
                     /locus_tag="ACG827_000667"
     CDS             complement(38205..39029)
                     /locus_tag="ACG827_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0482830.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(39040..40335)
                     /locus_tag="ACG827_000668"
     CDS             complement(39040..40335)
                     /locus_tag="ACG827_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0482831.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(40351..40830)
                     /locus_tag="ACG827_000669"
     CDS             complement(40351..40830)
                     /locus_tag="ACG827_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0482832.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40836..41864)
                     /locus_tag="ACG827_000670"
     CDS             complement(40836..41864)
                     /locus_tag="ACG827_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0482833.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41881..42987)
                     /locus_tag="ACG827_000671"
     CDS             complement(41881..42987)
                     /locus_tag="ACG827_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0482834.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(43216..45813)
                     /gene="adhE"
                     /locus_tag="ACG827_000672"
                     /gene_synonym="adhC"
     CDS             complement(43216..45813)
                     /gene="adhE"
                     /locus_tag="ACG827_000672"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0482835.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        13292 a         9755 c         8313 g        14570 t
ORIGIN      
        1 atctaacatt ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg gtttcgcttc
       61 tggatgtttg gctttatatc cttcgtaatc cttactgttg ataattgctt tcatattagg
      121 aattggatct tcacctggca tatattgcag attagaagca tgtaattttt tctcagaaac
      181 aaaccactca ttggaaaaga catgaatcaa atcatcactt acttgtgtaa aatagcgacg
      241 tttaatgaca aagatatctt cattgctatc tagtgtacct tcatttcttt gtgcttaaat
      301 tcaaagataa ttttctcaat aaagattttt gattccagta agattccttt actcctttta
      361 caacatagcg ttgtcctttg ttttgaaaca tcttcttccc ctctttgcca ttaaaaatag
      421 cccaattctc tataaagaag attaagcttc attattataa tataaagttt aaatttggtt
      481 tattacttac tttttcttat tctctagtaa gctaatattg aaaaaaatgt aatcagatca
      541 ttatttttat atgttgtaac ttcctgataa gaaaactaat taaaagaaag aggtaactac
      601 ataaactaat taaaaaagct aacttcatag ttggaggata aatggaagaa acttcaatga
      661 ttgtttctcc ttgtgttcct tcaatcgaca ctagtcctat tatcaatctc tcttttttgt
      721 ccatttactt ttgcgactgt tccccaataa tcaaatacag gaagcgttat tttttgtcct
      781 ttagattcat gtactgtata gactattttc gaatccatca cttgataagg catagtatgc
      841 cattccttgc caattcttac ttcatgattc tgcaaatgtt ctttgtattg taatgcttcc
      901 gtgggtgcat agtcataaag tcctccggga aggagtgtcg tgtaacgaat aagattttct
      961 tgattcatta ccaagtgatc tctctgagtg acaactttat gttcatagtt tgcttttgtt
     1021 gcagcagata cagccaacag taataaaaca attgccgtta tattaactac ttttctttgc
     1081 ttagctgatg tgctcttcat gaaaacgaac gataaaatca acgagccaaa cagtacttga
     1141 aagcctaaga ttcgccaagg aaattgaatc atttgaatgg gtgtattttg taataaagac
     1201 catggaaaaa atttggtgga taagagccaa gagactaccg cactaaggta aatcactttt
     1261 tctttgcgat taaatccttt aaaaaagaag agtggacaga tcaaagaaat aataccaatg
     1321 attcctaccg taaaggttgt caggtcatta ttcatcgcac caatcaaact atccgagaca
     1381 tttaatgctt ggtgttgtaa ggtcgtttta tctggctcat taatcgattg aaccatgatt
     1441 tgtcggaaca tcggatacca aaagtatgct gttaaaagaa tcgtcattcc tactgccttt
     1501 gacaactcca caatcaaccg gatgctttct ttttttcttg tttcacttac tacaaagccg
     1561 cctaagacta gtaagccaac ggctattaca ctcataaaag tggaaagaac atgtgtataa
     1621 atgagtaagc tcatactgac tgctaatgga attgcacatg gctttttctc ataaatcagt
     1681 gcgtaagcat aatacataac tatcggtata accgtaagtg caatcgcttc agcaatagca
     1741 gaacgatgat aaatatcaat cgtacggtac ccataaaatg tatataaaca gctaaatata
     1801 aaagccgcat cgattctttt taaaaatttt agtccataat gaaacatcag catgatagta
     1861 caaacattca atagccaaac atataagata taactaacga ttaaattatt acttatccaa
     1921 taaaaaatga ctgccggaaa aaaggtcaag tagggataaa aataattcac tccgctccca
     1981 taatgattaa acgtcgtaaa attgattgga ccagaaaaaa ttgaagataa ccctttgact
     2041 ctagcaaaat ggaaggcaat atcccccgat gtagaaatag tatgaacatt tctaaaataa
     2101 ttgattgaat aaagtccagc aataattgct aggagaatgg acaatattat ttttttcttt
     2161 acttgcatta cttaattata tactcctttc atggattgct ttatctagta taacttaaat
     2221 tttaaataaa tcgttattta tttttactct cacttaaaaa agcactaaaa ttataagaga
     2281 gggaggtaat ggtctgaaca ccaagaattg ttccaacatc gaattgctta actaatgagt
     2341 tcaaagctta attatgtggc tcttatattt accgagcgtg gattgcgatc tctttagcga
     2401 aacaaacatc tttcataaaa aaaacagcca tatccgcttt cttggatatg actatttact
     2461 actactttac tatctttcat tcttcttgtc tcacttatga taaggcgccc ccgcattgat
     2521 ccggaaggca cggtagattt gctcgacaag gatcagacgc atcaattgat gtggatacgt
     2581 catttttcca aaagagatct gagcattgct tcttttcatg acggcttcgc tgagtcctaa
     2641 agagccgccg ataacaaaaa caaggtggct ttttccttgg atacctaatt ggtcgatttg
     2701 tttagcaaaa gattcacttg taggattttt cccttcaatt gctaaggcat agacgtattc
     2761 ttgttctttg attttcgcta agatccgttc cccttctttt tcttttactt gtctcatttg
     2821 cgcttcgctt aaattctccg gggctttttc gtcaggaact tcgatcaatt cgatttttgc
     2881 ataggcattc agccgtttga catactcatt gataccttgg atcaggtatt tttcttttaa
     2941 ttttcctact gagataattt tgatattcat gttttctctc ctttggtctt catgctcact
     3001 ttgtttatta taatcaattt tttcgtctat atgtttattt tggctatttt tgttaaaaag
     3061 atttccccaa cagttatgca caacatccac aggtttatcc acagaaaaca gtgttatttt
     3121 accttatttc atcacattca ttaggcaaac atacgtttta taagaattta tccactttat
     3181 acacaggctg tggatatgtt ttatccctta tttttccaat cctatccgcg tagttttaca
     3241 cagtatctgt ggaaaaagaa aatcaaatat tttcatctga aaacaatgat ttttcctaaa
     3301 gtttttcata aaaagttcac agtctcaaag tagactaaag tcaacaaata agactatact
     3361 aacagtagat aaatgaatgt aattggagga ggaacacatg gatagaaaaa atgtgacacc
     3421 aaagatgaaa aaaagtaaaa ataatagcct ctggcgtaag ttaggtctgg ggcttgttgg
     3481 agggatcgtt ggaggactac tgacagctgg cattttttat gcagcgatgg gcactggcaa
     3541 caatagttct actgctacaa gcggcaatca gaattctgcc ggtgaaacag ttgtggaaaa
     3601 tgtcaaagtc aatgtagatt ctgatatcac caaagcggta gataaagttc aagacgctgt
     3661 cgtttctgtt atcaacttac aaagtcaaaa ccaatcaagc ggttttggcg gactattcgg
     3721 gcagcaagaa gaaagtagca gctcagatga tagtaatttg gaagcttata gtgaaggaag
     3781 cggcgtgatc tacaaaaaag acgggaatac tgcttatgta gtcacgaata atcacgtagt
     3841 agatggccag caaggtcttg aagtcttgat gaaagacggt acgaaggtaa aagctgaatt
     3901 agttggaaca gatgcttatt cagatttagc ggtgttgaag atcaattcag acaaagtcga
     3961 aacagttgcc tcttttggtg actcaagtgc tctgaaggtt ggtgagcctg cgattgcgat
     4021 tggttcccca ctaggatctg aatatgcaaa ttctgtcact tcaggaatca tttcttcctt
     4081 gaaccgacaa gtgacaagta cgaatgaatc gaacgaaaca gtcaatatca atgcgatcca
     4141 aacagatgca gcaatcaacc ctggaaattc tggtggtcct ttagttaata tcgaaggaca
     4201 agttatcggt atcaactcaa gtaagatcgc aagtacttct gaatctactt ctaatgtaag
     4261 cgtagaagga atgggatttg ctattccaag taacgatgta gtgaatatca tcaatcaact
     4321 ggaaaaagac ggaaaagtga cacgtccagc tctaggtatc acaatggtcg acttatctgc
     4381 cgtttctact cagcaacaag aacaaatctt gaagattcct tcatctgtaa ctaacggtgt
     4441 catcgtgaca aatgtccaaa cagctacacc ggctgaaaaa gctggattga aacaatacga
     4501 cgtcattact aagatcgatg acaaagaagt ttcttctggt gtagaacttc aatctgcttt
     4561 gtataagaaa aaagtgggag attctgtcaa aatcacttac tatgaaggct cgaaagaaaa
     4621 aacaactact gttgaattga cgatcgatca atcggcatta aaacaaaatc aaaatagtga
     4681 aaattaataa aaaagaggtt gtgacaaaga cttgtcacaa cctctttatt ttggaaatgc
     4741 ttctccgact acggcatcct caggcaatac taagattcct ttttttgctg gagcatttgg
     4801 caaatgcaat tcacgagcgg aacagatcat tccatagcta tctacccctc gcaaagttcc
     4861 tggccagatc atcattccat ctggcatcat agctcctggt tttgcaacga caactttttg
     4921 tccagcttcg atattgggtg caccgcaaac gatttgcaat actgtaccat tgtctacttc
     4981 tgtctcagta atcgataaat ggtcagaatc aggatgtggg acgcatgttt ttacgtaccc
     5041 gacaacgatc ttcggttcat gatcagcttc aagctgttct tcgaatcctg cttctttgat
     5101 caatcgattc aatttcgcta attcctcatc tgttaagctt acttggccgt tcccttcgat
     5161 tgttagatga tcggagatat ggaaaaagtt ccaagcaaca acttgacctt gttcatcttt
     5221 gatccgagca acattccatt tgcgttcaac agtgttttct gttcctttat cgtcggctat
     5281 aataaccatc aaggtgtctc cgacgtgtgc tttgttataa gcaaaaatca tgttctttcc
     5341 tccattatcc tttgctgaga tttatcttaa caaaaaattg cattcactgc atgaaatttt
     5401 tttcatgctt tatttccgaa gaagcctctt ctggaacacc atttattcgt aacaagggac
     5461 tgtgacaaga cttttgtcac agtcccttta ctgttttagt taattgatta aaaaggtcgt
     5521 acaagttcca cttgcttaat tacttggctg ttcttcttcg tcagaaacga tcaagacatc
     5581 acaaggtgaa tgacgtatca cgtagctggc tacacttcct gtcataaagc gttcgactgc
     5641 atttagacct gactgcccaa ccatgattag atcgatctca tatttttttg gcaatttgac
     5701 tgccatggat tcacgaacag aaccatatgc aataattcct tctatatttg tgtaatcaac
     5761 agatttccca tattctttac attcttcgat catttccttg gctgtttcag tttgctgatc
     5821 gactagattt tgatcaatag gtgcatagcc cataaagtta taaaattgct gatcgattac
     5881 gtttgctaca tatactttcc cattattacg gcgagccact tctaccgctt ttttgaaagc
     5941 agcaagtgct tgttcactac cgtcgatacc tactaaaata ttcttatatt cttgattcat
     6001 cttgacccct cctaacagat gtcagccttt tgttacactt tctaggaacg attcgatttc
     6061 ctgttttgtt ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac
     6121 gacaaaactt gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg
     6181 gtctacttca ataaattgcc aatcagaaaa gtcattttca atttcaggca tttgcggttt
     6241 gataaaacgg cagtctccgc accagccggc agtaaagaaa aatacactct ttccagtttc
     6301 tacgtagcct gctaattctt ctaaactatt tgggataatc attctttgtc ctctactttc
     6361 tgtttatgta tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc
     6421 tctttttata aaaaaaatct ggcaaaaagc aatcttttgc cagattttat agtttcacta
     6481 tcgcattatt ttccgtatac gatcgtattt actgttgatt tatccaaacc tttcagtgtt
     6541 tgaatcaaca tttctcgtgc agcttcaaaa tcgcgaatac tgaacatcgt ttgatgcgta
     6601 tggatataac gtcccactac gccaatcact gtacttggga ttccttcgtt ttgtgtatga
     6661 gcagcacctg catctgtacc gccttttgaa acaaagtatt gataagggat attgtttgtt
     6721 tcagcgatat ccagcaagta ctcgcgcaaa cgaggaagca tgatcaaacc tggatcttgg
     6781 atacgcatca atgtaccttc tcctaaatgt ccgaatgtgc catttttcgt cactgtatca
     6841 tctgctgcag agcagtctac tgcaaagaac agatctggct tgaatttatt gacagaagct
     6901 ttagaaccac gcaagcctac ttcttcctgg acattcgcgc ctgcaatcaa tgtatgacct
     6961 aatgtttcat tttgcaacgc ttctaaagct tctaatacca ttgtacagcc atagcggttg
     7021 tcccatgatt tactgataat attctttccg ttggctgttt tgatcgtttc agcgtaagga
     7081 acgattgtgt ctcctggaag gacaccgtaa ctcattgcct cttctcttga ttcaaaccca
     7141 gcatcgaaca aaatatcggt tacttctact gatttttgtc cgcttgttcc tcgcaataaa
     7201 tgtgggggaa cggaagaaga aatacatgga taattcttac cagtagaggt tttaagtgta
     7261 aaacgctgag cagaaacaac gtaaggattc cagccgccta gaggtacgac tttaaaaagc
     7321 ccgttttctt ggatttgtgt cagcatgaat ccgacttcat ccatatgtgc tgcgaccatt
     7381 acacggggtg catccgcttc ttcatgatgg cgcagaccaa aaatgccgcc caaaccatct
     7441 aactgtaact catctaccag cggttccata tgactttcca tataagcgcg aacatcttgt
     7501 tcaaaaccgc ttgttccttg taattctgtt aactctttga ttcgttgaaa tgttttttct
     7561 tccatatgct attcgtctaa atttcagact aaactcctct cagacttata ttgatctgcg
     7621 gataaaatat gattttttct gcagaccgat cgtatttatt atagcttatt ttttctggta
     7681 gcggttattt atttcaaaga aaatatgaga gtgtggtaaa ataagcacaa ggagtaacaa
     7741 gtgagaagaa ggaggtatgt ccacatgtat gaagaaagcg aactaagcta ttttaaaggc
     7801 gggttagcag ttggaatcgg tctaggatta ttgagcgggg tagcttcaac gatttggtat
     7861 catcgcaaaa aagtattgtc agcagaccaa gtacttgaaa atatcaagtc atcttttcta
     7921 aaagaaggtg caatcgaagg ttcttggatc tcttttgaaa aggaacctat taggaaattc
     7981 gccgttcggt cgagtgctta tcggggaggt ctttctcgaa tagaagatgg cgaaactgtt
     8041 tattatgaat ttttagcaga tgctcataca ggaaccgtat tagaaatttc aagaaaaaaa
     8101 ggtgcaacag attaatacgt caaaaaagtg agagaacgaa tacgcaccca ctattcaaaa
     8161 gagaggtatc atgtccttct tttgtagtgg gtggtttttt agatagagct ttccaaacat
     8221 atattccagt attttctttt taaataaatg atatttagcg ggaaaaacta gcatttcctt
     8281 tgattatgag gtatttttaa tttagctttt gttttctata gttaaggaga gtgaaaaatg
     8341 gaacaacaag aaaaaattgc tgttctgatt ccttgttaca atgaggaagc aacaatttca
     8401 acagtcattg cagactttaa acgagaatta ccagaagcag atatttatgt ctatgataat
     8461 aattcgaccg atcgaacata cgaattggcc gttcaaggtg gagcaatcgt aaaaaaagaa
     8521 cctcgtcaag gaaaaggaaa tgtgatccgc caaatgttct ttgatatcga tgccgactat
     8581 tatttaatgg tagatggcga tgatacttat cctgcagaag ctgtacatgg tttgctcgag
     8641 aaattacgtt ctggtgaagc agatatggtc attggtgatc gcctatcaaa tggaacctac
     8701 tttgatgaga ataagagacc tttccacgat tttggaaata accttgttag aaatacaatt
     8761 actagaatgt ataaaacaaa aattcttgat gtcatgaccg gctatcgagg attcaatcgt
     8821 atctttgtca aaagttttcc tattatgagt tctggttttc aaatcgaaac ggaattgacg
     8881 atccatgctt tagataaaaa attcaagttc gttgaaatcc caatcgatta tcgtgatcgt
     8941 cctgaaggca gcgaatcaaa attgaataca ttctctgacg gattcaaagt gatcatgatg
     9001 attattaaaa tgtttaaaga ttataagcct ttgcttttct ttgggatttg gaccatcttc
     9061 ttctttttat ttggattggt cacggggatt cctgtgattc gcgaatatat gctgacaagc
     9121 tttattacac gcatcccttc tgcgatttta agtactggat tgatgacttt agcacttctt
     9181 tcattagtaa cagggttgat tttggataca gtcgtcacga acgctaagaa agaatatgaa
     9241 ttgaatctat atcatgttta tcatgaatac cacagtagaa agtaaaaaaa ggatcaagac
     9301 tgcaaaaata atttgcaatt cttgatcctc tttttaatta attatttcgc caaacgatag
     9361 atggcatctg catagattgc tgttgctctg aacaagtcat caagactcat aaattcgttt
     9421 gcttgatgca ttgtatcagt atatcctggg aacatcgctc cataagcaac gccacgtttc
     9481 aacaaacgtc cgtaagttcc gccaccgatg atttgttcgt agcctttttc tccagtgtgg
     9541 tcttcataga cttgcaataa tgtagccacc aatggatcat ccattggtac gtaatgtggt
     9601 tccattacgc gagcacctcg agtaactgtt gcgccttctt gccccactgt ttgttcgatg
     9661 tcactttgca atccgtctac tgttacacct tttggaaaac ggaaattcat attgatgaag
     9721 ttcgcttcct ctgtaccatt ttcttcaaaa gcaaatagtc cagcattcat tgtcaatttg
     9781 cccatttttt catcttcata agcgactcct agtttttctc cataaaaatc ttcatggaca
     9841 taagcagctg ctacatggat aaattgttta gcagatccta ggaattcata gttatctaag
     9901 aacaatgcca agaaagaacc tgcattgata ccagattgtg ggcttgcacc atgtgcgcct
     9961 ttaccaacta attcgatttt tacatacgta ttgtccgctt caatcgttcc gcttactgga
    10021 tttgcttcga tgaattggta aaatgcttct tccatcgcga tagctgcatc tgcacttggt
    10081 acttgaagtg ctgcagttgc tgttcctggc accatatttt cgcgaagtcc ggaaacaaaa
    10141 gatttcaaga cataatctcc tgcattgtcg cctttgaagc gtaaagctaa agaaacattt
    10201 cctttttcac cattgatgat tgggaattca gcatctggtg agaaaccaaa atctggttct
    10261 tcttcatgtt cgaaataata agccatatcg ccccagccgc tttcttcatc gctgccgact
    10321 acgaagcgaa ctttttttga tagtttcaag tcaagttctt taatgatctt catcgcatag
    10381 taagctgcca tacttggacc tttgtcatcg cttgatccgc gtgcaaagat ctttccatcc
    10441 ttgattactg gttcataagg atctgtgtcc cagccatctc ctgcaggaac gacatccatg
    10501 tgtccaaata tccctagcgt ttcgtctcct tcgccaagat caatatgccc tgcatagtta
    10561 tcgacatttt ttactttaaa tccatcacgt tcgccataag ctaacatatg tttcaatgcg
    10621 tcgcgaggtc caggtccaaa tggtgcatct ggtgttactt ttgagtcatc gcgctcgctg
    10681 ttgacacgta aaagattttt caagtcttca agcaagtctt ctttacgtgc ttctacttct
    10741 ttttgccaat caattgtcat aaagttaatc ctccacttat ttaagtcttc gtgttcatga
    10801 taccacaaat tacagaaaaa aatagttcaa aaaatctgac aattcctttt cttatctgtt
    10861 tttctctaaa aaaaatatac gctataatga gtaaaaggag gtttttagat gctgataaac
    10921 gaacaactag tgaaaagagt catcacacct cgtccaaaaa tttcacataa aggaacattt
    10981 ggacgtatcg ttttgattgg cggcaatgaa caatatggcg gagcaatcat tatgagtgct
    11041 ttagctgccg tacacagcgg agcgggtctc acgactgtag taactgatga aaaaaatcgg
    11101 agtgctcttc acagtcatct gccagaagca atgattgtag tttggacgga tactgaccag
    11161 tttctttccg taataaagca agcagatgta ctcttgattg gtcccggatt gggtacttct
    11221 tctaagagtt tggaacgttt caaacaagta ctagaaaacc aatcaaaaga acaatggctg
    11281 gtgattgatg gctctgcttt aagtttactt gctgctaatc atcttttgct accctatccc
    11341 gaacaaactg tacttacacc tcatcaaatg gagtggcaac ggttgagttc tctaccgata
    11401 caagaacaac gggataaaga aaacaaaaag aagcaacagg aaatcggtgc tgtgctcgtt
    11461 ttgaaaagtc atgagacaaa aatttatggt caccagcact tctacaccaa tcctttaggt
    11521 tctgctgcta tggcaacggg cggcatgggt gacactttag ctgggatgat cacaggattt
    11581 ttagcccagt ttcctcaaaa tgaccaaacg attgcagcag ctatttatct tcatagtttc
    11641 attggagacc agctggcaaa agaacaatat attgttttgc cgacacaaat cagcgaagca
    11701 cttccgaaat ggatgaatta tttttgtaaa aaatcaatgg aaaacgaggc atgagtcatg
    11761 gagaaattta caggttatgt gcgagaacct ttttattatg aaacagatca aatggggatt
    11821 attcatcaca gcaattatat ccgctggttt gaagaggctc gggtagcctt attggtacat
    11881 ctagggtttc cctataaaaa aatcgaagaa tctgggatca tcatccccgt tctggaagta
    11941 aattgcagct ataaagaaat gatccattac ggagaaaaag tgagcatcca tccaactatc
    12001 caaaagtata cggggacacg gttggatttt agctacgaaa ttgtgggaac tgaagacaaa
    12061 aaattaaaaa ctactggcag tagtaaacac tgttttttac tttctgattc gcaacgatta
    12121 gtgaagctga gcaaagtcaa tccggagtta gatcagttat tccaagcgta cgcttctttg
    12181 gatgatgtaa attcttaata aagaccgtct aagcattttt cttgtattgt gtcgagtagt
    12241 tcgtataatg aaaagaaaaa agcaggtgat ccccatgtat gtcgttaaag taatgcacgg
    12301 ttacatcgat aaaactggct gtcgcactcg ggaaaaaaac ttagacaatc tgctcatttt
    12361 caaagacaaa aaagaatccg aagcttttgc taaaagaatc ggcggtcgtg tcaaaccgat
    12421 ccaagaggta cgaccagatt gattcaatat ttttcttttc gaggctctaa taggcatgcc
    12481 tatcagggct ttttttgcag actgtgtttt tatgcccaca cgatttgtaa gcgatttatg
    12541 ttataataga ttatatctgc atagaaagga aggttaaatg gttatcaata atttgcaaca
    12601 gcaagcttat gaatctatac gtaaacaaat catttattcc gagttagcac ctggaagtaa
    12661 aatctccgat aaagtgttag aagaacaact agctatcggg cgtacaccga ttcaagaagc
    12721 actgatccaa ctgcgcaatc aagagcttgt aaaaaccatt ccacagtccg gcacttatgt
    12781 ttcaaaaatc gatatccgat ctgcttcatt agcccgttat acgcgggaaa agttggaaaa
    12841 accgatcctg caagaatgca gtgcgaaaat gaccaagcaa gatcaagcta aactagaaaa
    12901 aatactagaa caaaccgatc aggcagtaat cgctcaaaat aaaaaagctt tttttgattt
    12961 ggatactgat tttcaccgaa cttgttatga aattgctgga aaacgagaaa tttgggattg
    13021 gctagaaagt tatagtacgc acttgaaccg tttcagatgg ttacgtttga cgatttcaga
    13081 attagactgg ggtcgagtat tagatgaaca tcagacaatg cttcaatcaa tgattgatca
    13141 taactttgat gaagtcggat ttctttgcac gatgcattta catatgatta tcgaggagca
    13201 agaatacgtg attcataatt accccgatta ttttgaagat attcaggaca gacccatcaa
    13261 ataagaggtt ttggaatcag cgttttgacc tgagtattag agaaagaaac ggcaaaatag
    13321 ttcctcatat tttgccgttt tttttgagct aataccgagg gtcagctgat tgcacaccgt
    13381 ttattgagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa
    13441 tagcttctta tgtttttaac ttggctggac ttattaccgc agatgcaagc ttttgaacac
    13501 cgttttccaa gaggtcgtga gcctgccgtt catctccgag tcacaaggaa caatttcaaa
    13561 aaacagcttc tcatgtttgt tgaatatttt gacttgtgac cgaggagttg acaggagaac
    13621 atcgtttatt cggaaaaaaa ggactgtgac acgacttttg tcacagtctc tttatttgta
    13681 atctaaatca tactccgtac atcttttttc tatttttttt atttcttcta agatcccttg
    13741 gctacgaatt tcattatatg gccaaagtaa gactgcctct aatacataag gcgaaaaatc
    13801 gctttgattc atctcttttg aaaaactcat cgctgctatc tcgctcaatg cacgaatgtt
    13861 tgattgatgt gtataaaaag gcaaccgcca cagcacaatt ttttcgcttt gcgtataaat
    13921 tcccttaaca gaagcttcga tgtggtgaaa tagttcatgt ccaataatca aagcttgcgc
    13981 tgtttcttta gagatcgaag ctagtcctga acattttaat ttttctaacg gctccgtcat
    14041 caatcgaatc ccttcatctt ctgcataagt tgcaaaagtg attcgttcgt ttatttgctc
    14101 tgattcacag gaaacgattg gaacagccat ttgttcacaa atttctccta catcggcaaa
    14161 agggtacttt tttttgatgc tacatgccgt ttcgatacca caatcgattg attgttgtat
    14221 aagaattttc tgcttggaca aaggaatctt tttccaaaat aaatctcgcc gaaactggta
    14281 gaatccccaa atttcttcgg ggatctgttg taattcgttt agtaactctt ctaacttatg
    14341 tttgtttgat atttccggca acgacgatca cttcttcctc cggttccata gtagatactt
    14401 ccagatccat caaaagctgt agttgttcca gatccgtcgc tgtaaaatca gagactcttg
    14461 ctcctaagca gagataaaat tctggtcggg caatcaattc agtttgataa actgccatat
    14521 cttcccaaag ttgttcgaca gctgtcatat aattggctgc agttgttttc atacatgaag
    14581 catgtccacg ttggaccttg ataaatcctt tttgatcgat gatccgaaga cttccagcat
    14641 tttttgaacg attctgttcg ccgcatacat aaaagaaagg tgtattttcc aataagcaaa
    14701 cttcattttt atttaatcgc atatcctctg cagcaagctc tagtgcttct tctgttgtga
    14761 tctccttggt tagatcggtt gctttcactt ccgtagaacc agtagcgata gccgttactt
    14821 ttgaagtctg tggatcgatt tctacgtgga cttcgatcga ttctggtgta gcgcctgatt
    14881 caatcgcttt attcatcgct tcatttttca acgatcgtat atcttcttta cttggcgaag
    14941 ggatgatacg ttctacaaca tctcggacca ttgcaagtgc tacaccaata gaagaaatca
    15001 cttcggcgtt ttcaggaatc gaatatttca cacccatttt attactaaag taagtaatca
    15061 atgaggcagc gccgccccct acacctacaa gagaaatctg atctttctcc aagtgatact
    15121 tgtctgccaa ttctaaaatc accggttcaa tcttagcata ggatttttcc atgatctgtt
    15181 ctgcgatgtc ttcaaccgtc gtatgacaat aatcagctaa tgcttggatg gcttttcgtg
    15241 cagatgggac atttccataa gaaaaatgtt cttcttgcac taatccaagg acattggctg
    15301 cacatgtatt tgtaatagtc acttcttctc cgtcttccat cactaccttg acgtaatcag
    15361 cagggtctcc ttctttagga gagaaaaatt caaccttagg acctttgatt ttttctgtct
    15421 ccgtaaatac tgcataatct agaccggcaa tatgtgcaga acgtggaccg acatcgatga
    15481 ttcctgactg attagcccgt accatcgaac cgcctgccac acctagaacc cgaacatcta
    15541 atgaagagat atatgttggg tggccaccta caattgaata atcgattgct ggacgtccat
    15601 ttttaatgac cccgatattg gttgttgttc cgcctacttc aaaataaaca ccattagacg
    15661 ctctcaagta catcaaagag cccatgactg atgctgctgg accggaaagc atggttaaaa
    15721 caggtcgttt tttcatttca ttgatttcca ttactccgcc gtctccgcgc ataatcatca
    15781 aagaaacatt gacccctgct tctcgtacgg aatcttctgt agaggttgcc gtatctagca
    15841 tttttggtaa aatagatgca ttgattgctg ctgtccgagt tcttcttgtc aatccatata
    15901 attttgtgat gtcagaagcc atcgttgtag ggatcgattt taccgatgct gcttcataaa
    15961 caactcgttc tggttcccca ttatctactc caaatgccat agaagaaacc aagacctccg
    16021 ctctttctct ttctaaagaa gaaatcgttt ctgaaacacg gtctacattt aagtgtttga
    16081 caggtaagaa tgcattgaca atctctattt ttttcttatt ccctaaatcg atatcgttca
    16141 atctagtttg tcgttttgct aaaaaacctt ctaatccacc ttttgccatc ccgattacgc
    16201 cgacttttgc tacgtctcct tcaatcagtg cattagtcgc ttgtgtggta gaatgtgcaa
    16261 caaacacaac atcttctgga ctgatattat tttccagcaa acaattttga aaagactgaa
    16321 cgacacctgc ggctactcca cggacatcat cgtgggttgt tttcactgaa gattttccga
    16381 tgatttcgtg cgtcgcatta tcgattgcga ctgctttcgt atgtgtaccg ccaacatcaa
    16441 ttcccattcg cacaaaacgt ttcattttct tttcctccat tcatctaatc gtttttatcg
    16501 acgttttgtt tactaggcgt tcccgtacaa taagaacgtg atcaggcata agatcgctcc
    16561 gatgatccaa ccagttggga tggatttctt catatattct cgagaagtta cttttgtata
    16621 acctaatccc caagcaaccc aagattgtgt aatatctaaa tgctgaggag caatcgttgt
    16681 gattgcaaat aacgggaaca aaaaggctgg cgacatcgtt ggattaacag ctaatacgac
    16741 agctaaaata gctgagcctg aaccgactag attcattgga ccgcggaaaa atcctagtgg
    16801 tgtcaatata gcaaatacga ttgccaagac taatggtgat tgcggtatcc aatctccaaa
    16861 aatagctgaa aaataaggag aagcatacgc agctgaatta ttgaacatcg ctaaggtcat
    16921 caagaaacca accattggcg caacatctac tgcgccatct gtaaataatt tcgcaaactg
    16981 acgacagatt tctttataac ctccgcgcaa ttttccagta aacagcaatg cccagatacc
    17041 tgctagaata aaacctaaga tgattggaag atctaaaaga acgaccccta aaactggtaa
    17101 taatacggct aaccatgaga tcattggtgc gtcgccacct tcagctggta cgtttgctgc
    17161 cattgcatag cgtttctttt tgttcattga tatattagcg accgttagta ccacaactaa
    17221 gcttacaatc atcccaatca taccaatttg gaaatagtca ttataagtat agctttcagc
    17281 cgctggattg aaaccagcat aaatcgtttg atattgtttg aaattgacga tattagcaaa
    17341 gattccagcc ataatcgagc ccataaatga gaacagtgca accggtactg aaatacctaa
    17401 tgacatcaag attggcaaca caataactgc aatagaaatc actggtccga ttcctgtcat
    17461 cgacatgaat ataacagcgg ttacaataca tagtaagctc atcgtgattc ttggcttgtc
    17521 ccctccaagt tcaactacct ttcgaatcag ggtagctgcg attccgctat ctactagaac
    17581 acgcccgaaa aaggcaccaa agaagacatt taccaagatc gatttggcgt actcagctgg
    17641 ccccgtcgta tagatatgcg tcaacacatc aattactcct tgcccttcca tcgccgggtt
    17701 tggtgaaaac gtgtttccga ctagcgctaa tcccatccat acagtagcca tgatggcaaa
    17761 gccgaccatc aagttgtagc cttttacaca gtaccagacc atgaaaaaga atgagataac
    17821 taacaaaata ccaataataa tatcactcat gttattcctc cgtccatttt gtttttctga
    17881 taagcaaact aatatatcag tcaaactcat tctaacttat gtagttagcg ctttcattaa
    17941 agggtgacca taaatgttca gttttcaatt aaaaaacctt aattatgcta ttttagactt
    18001 atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga ggaaagaaca
    18061 taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc caaagcttct
    18121 tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa gtcagctaac
    18181 attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt tgagtagctc
    18241 aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg gaagtcgcgt
    18301 ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg ttcttttgct
    18361 gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag cactctttta
    18421 cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc ctcctaataa
    18481 aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta gttgttggcg
    18541 aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac ggcgttgttt
    18601 ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca atccgccttt
    18661 accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg gcatgactat
    18721 atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc agttagtcat
    18781 tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg tccgtccatt
    18841 ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg attcaagact
    18901 ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga agctttcact
    18961 ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa gtcattcaag
    19021 tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt tttacgcgct
    19081 tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat tcgcgcaact
    19141 ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc tgcaatattc
    19201 aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat caatcgtaac
    19261 tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat tcacctccaa
    19321 atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg agttctctta
    19381 cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc cagtgacatt
    19441 atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca tagtatagca
    19501 aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct gaaaatgatt
    19561 agattagcag actttcttgc agtgattccc gcttctttgc tatactaaag aaaaacgagg
    19621 tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct tacaaaggga
    19681 gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg gggtgaccaa
    19741 agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa acttacgatg
    19801 caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa atgtgatttt
    19861 tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca atgtaaatta
    19921 acaggtacga attttgccga aagttatctt cgagactgta ctttcactga ttgtattgct
    19981 tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca ttgtcaatta
    20041 gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga caatcagttg
    20101 accggttcga actggtttcg aacttctctg aagggattag actttacgac taatttcttc
    20161 acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca agagcaagca
    20221 cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga atccgccgcc
    20281 acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg attccccatc
    20341 cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct gtcacaatag
    20401 ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca ttcaagttgt
    20461 tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa gctaagttcg
    20521 tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct ttttcctttt
    20581 ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact acattttgtt
    20641 ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc agcagattct
    20701 tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta ttcacttcct
    20761 cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat aaactgtata
    20821 cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg tactcggtat
    20881 tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata ggactagttt
    20941 cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt aacgcttcat
    21001 caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa gtcagcatct
    21061 cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg ttttgctgta
    21121 cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata ctctatcctc
    21181 ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta ccctttattt
    21241 gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat ttttcttcga
    21301 tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag cggtctccat
    21361 cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa cgtctgttca
    21421 attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac gaacgaccac
    21481 gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact aataaatccg
    21541 gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc agttcatcat
    21601 acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg aaaagcagtg
    21661 aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct tgtttgatgc
    21721 ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt tcagggtttg
    21781 cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc tgactatcaa
    21841 agtgttcagc catcatttct gttaaacttg attttccggc accaattgta cctgctaaaa
    21901 cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt aactccaacc
    21961 tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta gtcaaagaag
    22021 aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac gtattcttag
    22081 ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag acccttagtg
    22141 gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt tgcttaaaaa
    22201 tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag aatcaagcgg
    22261 aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga ttgcctttcc
    22321 tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg acgatcaact
    22381 ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa aaaatatcaa
    22441 aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc aactgaccat
    22501 tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg ccacagcagt
    22561 cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac tattagaaaa
    22621 tgtccagctt tcggaaaaaa tcgcccatgg taatcctagt ggaatcgatg cagccgctac
    22681 tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact tttctttgaa
    22741 catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa gagaagccgt
    22801 caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa tgatccaaaa
    22861 attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac cagaaacgtt
    22921 ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca gcaatgattt
    22981 tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca aattaactgg
    23041 cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag cccaagaaat
    23101 cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat taggagtaca
    23161 tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc aaatattggg
    23221 gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta acattggatg
    23281 cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat gaattttatc
    23341 tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt cttgaccttt
    23401 ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat ttcgttccta
    23461 ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc gcttgcaata
    23521 ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga cgcgggtctg
    23581 gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc catgacgact
    23641 taagttctta cgctaagcca gtcccttccg attctttcga agacgattta gcaatggttt
    23701 tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg cgtcggacag
    23761 tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat ctatatcaat
    23821 tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg gaaagaaacg
    23881 gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac tggtctcctg
    23941 acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt ccatgctact
    24001 ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat ttatctgagg
    24061 tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca catgctggtc
    24121 caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct gcaccaggca
    24181 aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca gttatcgctg
    24241 cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga agtatccaat
    24301 ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa ttagttttag
    24361 atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact gaaaagtatg
    24421 cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt gagttagaca
    24481 gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt gcaacggtca
    24541 aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc aagattgcag
    24601 cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct gctagctgtt
    24661 atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa caagaaactc
    24721 agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt gagccattga
    24781 ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc tctacttctg
    24841 atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat cagcttttct
    24901 taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa aataatgtaa
    24961 cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta tctgcaatca
    25021 ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct gaacagtatg
    25081 aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta attgttgacc
    25141 agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc actccactgc
    25201 cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag acgaacatgt
    25261 ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg tacggatcat
    25321 ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac aggcagttgg
    25381 gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg aaaaaacaaa
    25441 aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga ttgccactgg
    25501 ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga ttatgcgaca
    25561 agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg tcgaaagagc
    25621 caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga atgcacctca
    25681 agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac ttattcaaga
    25741 aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg gcatgacaag
    25801 agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca gcggtcgaag
    25861 cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac tgagttattt
    25921 agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag cagacacttc
    25981 tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta aagccctttg
    26041 tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga tgaatcacgg
    26101 cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc tgctttatac
    26161 tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct tttccggacc
    26221 agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa gacgcacaga
    26281 aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtacaaacg
    26341 gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa
    26401 ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc ctcttttttc
    26461 gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg atttcacaac
    26521 ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat aggcaagtat
    26581 gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag aagccaagaa
    26641 gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg tacgtccaac
    26701 gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta atggagcagt
    26761 ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt attgaccgta
    26821 ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag cataagcact
    26881 gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt taccgccgcc
    26941 tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat ttaaatccac
    27001 gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc gtttgatgat
    27061 ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct gttgaaccat
    27121 cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat cgatgcctgc
    27181 ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat taaatacgat
    27241 ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat agacagcacc
    27301 ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt ccatcatttt
    27361 ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct gtataggtga
    27421 tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca cctgaatgga
    27481 tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt tttgtttcat
    27541 ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca aagtagacat
    27601 aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg cttcctggtt
    27661 ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga tggatgtatt
    27721 ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt tttacttggt
    27781 cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta atatagaatt
    27841 tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct tttccgatat
    27901 agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg aatcctggtc
    27961 caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca cctagttctt
    28021 tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag atcattcgat
    28081 cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct actaaaaatt
    28141 cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt gtataacgtg
    28201 tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca gagccgcatg
    28261 ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg atcgtatgct
    28321 tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata ttggataaaa
    28381 tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc acgattgggg
    28441 tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc cccttcaatt
    28501 cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga atattcggga
    28561 acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct tctggcgctt
    28621 tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata tctactaaag
    28681 gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt ccacgatagt
    28741 tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc tgtttcgtca
    28801 ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat atatctattc
    28861 cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa cttgatccgg
    28921 cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata taataagtac
    28981 ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat tgcaagtgct
    29041 ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc gcttcatgtc
    29101 cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg gcagcgaatc
    29161 gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg atttctccga
    29221 gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt gctgcatact
    29281 gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct aaaaggttcc
    29341 cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg tccagactca
    29401 caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca gaaatgatac
    29461 ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt ttcagcgctg
    29521 ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac agattgcaca
    29581 tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca gctaagatag
    29641 ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt tctggttggt
    29701 ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact gtaaaagtca
    29761 actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa gcttcttctt
    29821 tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat aaaatatcaa
    29881 tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa cttggaaatc
    29941 tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat tatttttgaa
    30001 ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt tcttttgtct
    30061 catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt tgttcgtttt
    30121 tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg tcaaatgcga
    30181 tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg gtgatcgtat
    30241 attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc tcttcactga
    30301 attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg attccgattt
    30361 ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac aagatctcat
    30421 tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc tgttctaaaa
    30481 acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg tagcgaataa
    30541 gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg atcatcaaca
    30601 aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt gcgatcgtct
    30661 gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct ttgatcaata
    30721 cagttccata aggcttttta ttttccatat aatattctcc tatttcttta taagaaaaat
    30781 tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc atttctcttt
    30841 acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga tataaagcaa
    30901 tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct cgcaaagcat
    30961 cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg gtttctggga
    31021 ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt acatcttcta
    31081 aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg tacttttctc
    31141 cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac gctacgccat
    31201 gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt cctaaaccga
    31261 catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct cgcccatttt
    31321 gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct tttaaatgca
    31381 gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca tgcgtcatag
    31441 catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata tcgctgtcga
    31501 tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg tgatttactt
    31561 catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc gttggtatag
    31621 ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta atgacgtctg
    31681 aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt ggcgaacctc
    31741 caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact ccatctttta
    31801 cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg attgctgctt
    31861 tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct ttgtcagtaa
    31921 tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac tcaatcgctc
    31981 cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc tattcctcct
    32041 aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt tgcccagttg
    32101 cttgttgcta cagttcaaag actggcatca attcccaact gactggagag gcatctggat
    32161 ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat gtatttgcca
    32221 ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa cttgtactcg
    32281 gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta cgcatttctg
    32341 gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa actttcatgc
    32401 aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc caaataacct
    32461 gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc cgtgattgtt
    32521 tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt ttctgctgtc
    32581 tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc atgttgtggc
    32641 cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat ctcatttgtt
    32701 cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa gcattccgta
    32761 cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt aaaactcatc
    32821 gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc tacttctaaa
    32881 cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca ttcgttttcc
    32941 agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat tccaagattc
    33001 tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac aataaaatag
    33061 catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac tttttcagga
    33121 tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc gccatttctt
    33181 tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt cacataaggc
    33241 gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta attccaacac
    33301 gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa cttgattttt
    33361 gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt ccgtataagc
    33421 cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa ctggttccag
    33481 catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag cagcgatacg
    33541 gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca attcgttacg
    33601 gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat ggtctgaatc
    33661 ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag ctaatgaaga
    33721 taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac aaacaagttt
    33781 gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat aagcttcagc
    33841 gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat ctaactcttc
    33901 tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac gatcaatcgg
    33961 attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc cagtttcttt
    34021 cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt attcacgaac
    34081 ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg aaaagaaagt
    34141 cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt tttcaaaatg
    34201 tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga tagcatgaag
    34261 gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt gtctaagctc
    34321 ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac cggtcaattc
    34381 gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca tagaaatttt
    34441 gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg catatttttc
    34501 ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt gattttattc
    34561 acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc acatggaaac
    34621 gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt actgagcatt
    34681 tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac aatgattccg
    34741 gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc tacgatgtgc
    34801 agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg tgcgtaacaa
    34861 agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg gactggctgc
    34921 tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt caaaaatcga
    34981 gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag ctctgccaat
    35041 tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa ccacatcccc
    35101 atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt ataattttca
    35161 gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag cgaccatgtc
    35221 ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac agctgacgcc
    35281 gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt tgaaaattgt
    35341 tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa taattccttc
    35401 gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc attcagcagc
    35461 tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac ataccctaaa
    35521 taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc ttcagcaaac
    35581 aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata caacttatct
    35641 agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc ccgataagca
    35701 atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac tgcccatgta
    35761 tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa tccaatcaaa
    35821 atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt ccgaaagccg
    35881 tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct cgtacttttc
    35941 attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt catttctcca
    36001 cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat ttattgacaa
    36061 tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata taattgcatt
    36121 atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc actcatggtc
    36181 ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt tattttttta
    36241 tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac gagcacattt
    36301 accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat ataatgataa
    36361 tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat atcagcgtta
    36421 tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg gtcagaacgt
    36481 ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt caacaggaga
    36541 tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc aacaaggcac
    36601 gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga atgaactgat
    36661 tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg tttacaaact
    36721 gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat tagaacaaca
    36781 attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg gttctccacc
    36841 aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc ggtatacaga
    36901 atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt actttagccg
    36961 cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca gctgattcat
    37021 ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa taaaataacc
    37081 gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta gtgatcattc
    37141 tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat cacgtttcaa
    37201 aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat gcggataaga
    37261 agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca acaagtgctt
    37321 ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag tgtacttttt
    37381 aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt cctctaaaga
    37441 aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac gctcattagg
    37501 aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat tgagaaaaat
    37561 caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt ttttatcagg
    37621 aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt gcaatacacg
    37681 actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg gcgtggaacg
    37741 actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat ctgaagattg
    37801 gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg ctttgatcgt
    37861 aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat ggatctcata
    37921 gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga catgatagat
    37981 ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct tttggagcat
    38041 gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt ttttgtaatc
    38101 aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc ttctagcccc
    38161 gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc aagaagactt
    38221 cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag gtacggacga
    38281 ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc caaatttacc
    38341 ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca tatcttttca
    38401 ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc actacttgtg
    38461 atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc ttggatcagc
    38521 tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat ccccaccatc
    38581 atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc ttcagcaaat
    38641 gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca tccagtttcc
    38701 gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc gaactttgtc
    38761 agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca acttaaaatt
    38821 cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat cccagaattg
    38881 atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag ctgcagtgta
    38941 tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac ttcggaaaga
    39001 tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca tattgggtag
    39061 ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga taaccccata
    39121 aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac ttgcaccgac
    39181 atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca tgattgaaaa
    39241 caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa atattggtgt
    39301 aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata acacaagatt
    39361 gactaataac agaataataa atttattatc cgttacgccc ataatcaacg aactaattgc
    39421 ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag ccaataagaa
    39481 catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat cttttagttt
    39541 taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta tcgctgaagc
    39601 ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa tcaatagaag
    39661 gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt tttcgacaac
    39721 agggtattta ttctttttag caacgatgaa tgctactagc atgattccta gcgcccacaa
    39781 agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac cagcaacaag
    39841 tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag ctgacgcgat
    39901 attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag ggatcaacac
    39961 tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa ataaagcatt
    40021 ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca acatcgcaaa
    40081 atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga gaacgaaaaa
    40141 cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag ctgaactaaa
    40201 agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga atgcaatact
    40261 tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata acactaatcc
    40321 ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg ctggcttgtt
    40381 gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc ccgatacagg
    40441 tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct gccccattgt
    40501 caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata ccagaaataa
    40561 actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt cacttaaaac
    40621 cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca tggcaaacca
    40681 gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta aaaccgttct
    40741 ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag ctcccaggat
    40801 ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc ttggatcttt
    40861 tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg ggcaactgcc
    40921 tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc ttgtgctaaa
    40981 gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt tgattctttc
    41041 gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat ggcatcattc
    41101 atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt agccacacca
    41161 ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat caaattggat
    41221 tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc taaaagctct
    41281 accattttga tagcggtttc gctttgcatc acacggatct tctttccctt caaatcttcc
    41341 ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc ataataggtc
    41401 aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat ttcttcatta
    41461 tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga aaaaatcgaa
    41521 taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc tactgctcct
    41581 gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg gaaatactgg
    41641 atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt cattgcatca
    41701 attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc atttacatct
    41761 gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc tagcagacct
    41821 aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt ttcctttctt
    41881 ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact gtccataagg
    41941 cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc ctgtcccgat
    42001 tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt ggatagcacg
    42061 ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt tcaaaatacc
    42121 gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat ccaacaacgt
    42181 atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg cttcaagtgt
    42241 ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt caatgatctc
    42301 tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg catagttatg
    42361 atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca tcaagtattt
    42421 gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat tcaacagttt
    42481 cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca actgattctt
    42541 attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag gcatgtcatt
    42601 catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt tcgtgtcttc
    42661 atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg gcggagcgcc
    42721 ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag gattttttgt
    42781 catctggtaa tttttataaa taccatatag accgacacct tgtggccaat tccacactgt
    42841 ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac cattatcgtc
    42901 tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta attctttagg
    42961 tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc tttcatttta
    43021 tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa caaaaactgt
    43081 ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg aaattaaaaa
    43141 aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt atcttttttc
    43201 tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca attcagagat
    43261 caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt cagccatacg
    43321 gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac tcatgttgat
    43381 gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta attcctctgt
    43441 tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat ctgcacggaa
    43501 atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat agcggataat
    43561 atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc cgccgatttt
    43621 atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac cagccattgt
    43681 agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg atttttctaa
    43741 atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat ctgaagccat
    43801 gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat ctgctgtgac
    43861 agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg gcgtcaatgc
    43921 ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg caaatggcgt
    43981 tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt tttctggttt
    44041 gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac caaagaaaga
    44101 tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg cagaaccgcc
    44161 tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc ctgcgtatac
    44221 ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca catcattttt
    44281 acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca taccagggtc
    44341 acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata gagagttctt
    44401 ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc gtttcgctac
    44461 agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt tcttaccata
    44521 agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac cgatgccgcc
    44581 ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac cgaatttgac
    44641 atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc caagattcaa
    44701 cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca tggcaagaac
    44761 tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca attcagcaat
    44821 cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt ccatcgcaga
    44881 atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg catcttctaa
    44941 tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt ctgcttttac
    45001 tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa tcattccatt
    45061 atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg ctgttttttc
    45121 aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg tagagtaagc
    45181 agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg gatggttcat
    45241 tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat tttcaggagc
    45301 tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag atttttgggc
    45361 acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca ttgatttgaa
    45421 aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac cgactggttc
    45481 agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg ttttatcatg
    45541 tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt tatcttcgta
    45601 aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt gatccaaagc
    45661 tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc tttccatttc
    45721 tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta cgtcaattgt
    45781 cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg gtcatggtta
    45841 tttgttaaat aattcacatg tatatcatac aatatttatt tttctatgta aacacttttt
    45901 tgtgattttt ttcacagtta tttttaaata
//
LOCUS       DAZKOU010000013        44883 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000013 DAZKOU010000000
VERSION     DAZKOU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.013"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1054)
                     /locus_tag="ACG827_000673"
     CDS             complement(44..1054)
                     /locus_tag="ACG827_000673"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0482836.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1234..1578)
                     /locus_tag="ACG827_000674"
     CDS             complement(1234..1578)
                     /locus_tag="ACG827_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0482837.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1571..2005)
                     /locus_tag="ACG827_000675"
     CDS             complement(1571..2005)
                     /locus_tag="ACG827_000675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0482838.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2143..2880
                     /locus_tag="ACG827_000676"
     CDS             2143..2880
                     /locus_tag="ACG827_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482839.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2873..4084
                     /locus_tag="ACG827_000677"
     CDS             2873..4084
                     /locus_tag="ACG827_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0482840.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4288..5070
                     /locus_tag="ACG827_000678"
     CDS             4288..5070
                     /locus_tag="ACG827_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0482841.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5140..5802
                     /gene="trmB"
                     /locus_tag="ACG827_000679"
     CDS             5140..5802
                     /gene="trmB"
                     /locus_tag="ACG827_000679"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0482842.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5900..7654)
                     /gene="ade"
                     /locus_tag="ACG827_000680"
     CDS             complement(5900..7654)
                     /gene="ade"
                     /locus_tag="ACG827_000680"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0482843.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7786..8715
                     /gene="comGA"
                     /locus_tag="ACG827_000681"
     CDS             7786..8715
                     /gene="comGA"
                     /locus_tag="ACG827_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0482844.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8672..9721
                     /gene="comGB"
                     /locus_tag="ACG827_000682"
     CDS             8672..9721
                     /gene="comGB"
                     /locus_tag="ACG827_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0482845.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9718..9996
                     /gene="comGC"
                     /locus_tag="ACG827_000683"
     CDS             9718..9996
                     /gene="comGC"
                     /locus_tag="ACG827_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0482846.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9971..10441
                     /gene="comGD"
                     /locus_tag="ACG827_000684"
     CDS             9971..10441
                     /gene="comGD"
                     /locus_tag="ACG827_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0482847.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10413..10760
                     /locus_tag="ACG827_000685"
     CDS             10413..10760
                     /locus_tag="ACG827_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482848.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10723..11148
                     /gene="comGF"
                     /locus_tag="ACG827_000686"
     CDS             10723..11148
                     /gene="comGF"
                     /locus_tag="ACG827_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0482849.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11120..11479
                     /gene="comGG"
                     /locus_tag="ACG827_000687"
     CDS             11120..11479
                     /gene="comGG"
                     /locus_tag="ACG827_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0482850.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11651..12658
                     /locus_tag="ACG827_000688"
     CDS             11651..12658
                     /locus_tag="ACG827_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0482851.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12683..13867
                     /locus_tag="ACG827_000689"
     CDS             12683..13867
                     /locus_tag="ACG827_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0482852.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14304..15887)
                     /gene="abc-f"
                     /locus_tag="ACG827_000690"
     CDS             complement(14304..15887)
                     /gene="abc-f"
                     /locus_tag="ACG827_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0482853.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15992..16120)
                     /locus_tag="ACG827_000691"
     CDS             complement(15992..16120)
                     /locus_tag="ACG827_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482854.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16465..16608)
                     /locus_tag="ACG827_000692"
     CDS             complement(16465..16608)
                     /locus_tag="ACG827_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0482855.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16831..17310)
                     /locus_tag="ACG827_000693"
     CDS             complement(16831..17310)
                     /locus_tag="ACG827_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0482856.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17383..17667)
                     /locus_tag="ACG827_000694"
     CDS             complement(17383..17667)
                     /locus_tag="ACG827_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482857.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17745..17939)
                     /gene="ssrS"
                     /locus_tag="ACG827_000695"
     ncRNA           complement(17745..17939)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG827_000695"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(18011..19297)
                     /locus_tag="ACG827_000696"
     CDS             complement(18011..19297)
                     /locus_tag="ACG827_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0482858.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19435..20106
                     /locus_tag="ACG827_000697"
     CDS             19435..20106
                     /locus_tag="ACG827_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0482859.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20072..20560
                     /locus_tag="ACG827_000698"
     CDS             20072..20560
                     /locus_tag="ACG827_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0482860.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20573..21520
                     /gene="prmA"
                     /locus_tag="ACG827_000699"
     CDS             20573..21520
                     /gene="prmA"
                     /locus_tag="ACG827_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0482861.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21521..22285
                     /locus_tag="ACG827_000700"
     CDS             21521..22285
                     /locus_tag="ACG827_000700"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0482862.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22518..24731
                     /locus_tag="ACG827_000701"
     CDS             22518..24731
                     /locus_tag="ACG827_000701"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0482863.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24742..25188
                     /gene="dtd"
                     /locus_tag="ACG827_000702"
     CDS             24742..25188
                     /gene="dtd"
                     /locus_tag="ACG827_000702"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0482864.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25359..26618
                     /locus_tag="ACG827_000703"
     CDS             25359..26618
                     /locus_tag="ACG827_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0482865.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26707..27654)
                     /locus_tag="ACG827_000704"
     CDS             complement(26707..27654)
                     /locus_tag="ACG827_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0482866.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28048..29340
                     /gene="hisS"
                     /locus_tag="ACG827_000705"
     CDS             28048..29340
                     /gene="hisS"
                     /locus_tag="ACG827_000705"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0482867.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29355..31124
                     /gene="aspS"
                     /locus_tag="ACG827_000706"
     CDS             29355..31124
                     /gene="aspS"
                     /locus_tag="ACG827_000706"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0482868.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31233..32108
                     /locus_tag="ACG827_000707"
     CDS             31233..32108
                     /locus_tag="ACG827_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0482869.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32374..34257
                     /locus_tag="ACG827_000708"
     CDS             32374..34257
                     /locus_tag="ACG827_000708"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0482870.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34332..34616)
                     /locus_tag="ACG827_000709"
     CDS             complement(34332..34616)
                     /locus_tag="ACG827_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0482871.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34606..35340)
                     /locus_tag="ACG827_000710"
     CDS             complement(34606..35340)
                     /locus_tag="ACG827_000710"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0482872.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35469..36092
                     /locus_tag="ACG827_000711"
     CDS             35469..36092
                     /locus_tag="ACG827_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0482873.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36284..37405
                     /locus_tag="ACG827_000712"
     CDS             36284..37405
                     /locus_tag="ACG827_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0482874.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37412..40540
                     /locus_tag="ACG827_000713"
     CDS             37412..40540
                     /locus_tag="ACG827_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0482875.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40559..43753
                     /locus_tag="ACG827_000714"
     CDS             40559..43753
                     /locus_tag="ACG827_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0482876.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43899..44606)
                     /locus_tag="ACG827_000715"
     CDS             complement(43899..44606)
                     /locus_tag="ACG827_000715"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0482877.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14398 a         7967 c         9414 g        13104 t
ORIGIN      
        1 acaaaaaaag cgagacataa gtcttcgctt tttttgtttt cttttatttt gatgaatcag
       61 ttgtttcttc agttgaagat tcttttgttg aatcagttga ttttgtgtca gaactctttg
      121 ttgaagctgt agtttcactt gagtctttcg tacttgatga agttgttgtg aatgcactca
      181 acacattttc aaaggcatca tctttgattt ttacatttgc atcttttaat tcttcaccga
      241 ttactttagt tgtgaatgca ttgtcactta gtttagtttc agtagcgata tcttttagtt
      301 gatctttgta tttatccatg tcattgcctt tattttggtt tttcaccatt ttgacaacgt
      361 agtattctgt cgcataagaa gtaggatttg ttgtagtaat aacatcggag atctcaccgt
      421 ctttcaattt gaaagctgct tcttttactt ctgcaggaat agttgttgtc gttgagtcga
      481 atttaacttt accgccatct tcttttgtct ctgtatcagt tgatttgtct ttggctaatt
      541 tagagaaatc atctccatca tccgctgatt ttttcacatc ttttgcttca tcttcacttg
      601 ataatttgat gatttgtgct tctacttctg gatggaaaga tttccaagca gttttcaaat
      661 cgtcatctgt aatatctacg tgagctttca agccagcttc aaaagctaaa ttgttacgaa
      721 tatattcttt ataagtatct ttcgtatatc cagcagcttc taattgtgat tcaaaagtat
      781 cacctaatga tttcgcttgt ttgtcgtatt ctgcatccac ttgcttatcg gttactttat
      841 cgccatattt gttattgaat actttataaa tgatcatccg ttgtactaag gattggttcg
      901 atgattcaag ttttgcttca tcatagaaat cagatacagt gatcgttccg cctttcatcg
      961 ttgcgatatc tttattagta tcgcctgaac aagctgctag agttaaaaca gccaacgcac
     1021 tagtagctgc caaaataatt gatttttttt tcattaaaat ttgcactcct gtaattagtt
     1081 attattttta tatattaact ataccatatc ccatttcctt taaaaacgtt gtcgtggtag
     1141 tttcaaacaa aattcaaaat ttcatcacat tcctaggaaa tacgcaaact tttctcactt
     1201 atatagttaa atctgttgag aaaataatca ttattatatt tgttcaggat actctggcag
     1261 atcagctttt agtttttcta cttgttcatt gatttgtgtg atacgtggtt cggcttggaa
     1321 tttaaaatct tcgatatctt ttttgattgc ttcttggaaa gcaggaatca cagattcagc
     1381 tgtttctttt gtttcgatca atgcgctttt aaaatgattc aggctgttat ttaaatcatt
     1441 tgtcagagtt gttgcttctt ccaattcttc gatcagtttc tttcttgttt cattcccgct
     1501 cctgggcgcc aataacagac ctccgacagt gccagcagca gctccaaata acagtccttt
     1561 gaaaaagttt ttaatcatcg caggacacct gctttacgat tgtttcttga atagctttta
     1621 gttcttccga tccataacta tcttgatgat tgacaaagtg aatagaaaaa tcatcctctt
     1681 tgctgtagcg agggactaaa tggatatgtg aatgaaaaac agattgatag gctagttcac
     1741 ggttgttatt caagatatta agacctttca tttctggaaa agctttttcc aatgcttgag
     1801 caatcttagg cacacgcgaa aaaacctctc ctgcaagttc gggatcatat tcaaaaatat
     1861 ctgctacatg acgtttaggc acaactaatg tgtgtccttt cgttgcttga gagatgtcta
     1921 aaaaagcata gaccttgtcg tcttcatata ttttatagct tgggatttct tgattgatga
     1981 ttttgcaaaa aatacagttt tccatataaa acatcctttc tataaagaat aaatacttca
     2041 gcttaacttt accataagtt aaagctattg taaggaaaaa aacaaaatac cgtcattttc
     2101 ttcgtggtat aataaataaa aataaatggc ggaggaaaaa gaatgagttt aattatcgat
     2161 catgtcacag gggggtacgg acatctccct gtgttaaaag atatcagttt cgatgtacag
     2221 cctggagaga tagtagggtt gattggactg aacggagcag ggaaaagtac gaccatcaag
     2281 cacattatcg gtctgctgga agcttcaaaa ggaacgatag aaatcaatca tctatcctta
     2341 aagaaaaata cggaagaata tcgcaaacaa attgggtata ttccagagac tccttcttta
     2401 tatgaagaat tgacattgag agaacatatc gaagtcactg cgatggccta cgatatacct
     2461 atggatatag caatgaaaag agcagaaaag ttgttggaga cttttcggtt atctaatcgt
     2521 cttgactggt tcccagctaa tttttcaaag ggaatgaaac agaaagtgat gatcttatgc
     2581 gcctttttga tcgagccttc tttatatatc atcgacgaac catttttagg cttggatcca
     2641 ctagctatca atgcattgct tgaattgatg gtagaaatga aaaaagaagg agccggtatt
     2701 ttgatgtcta cgcatatttt agctactgca gaaaaatatt gtgatcgttt tgtggtctta
     2761 cataacggag agatacgagc agacggttct cttgagatgt tgagaaaaga atttcagctg
     2821 ccggattctt ctttggacga aatctatatt gctttaacaa aggagcaggc acatgagtag
     2881 tttttttcaa aaacgtttag caagacatca aaagaaaatg atgaaatata tgcgttatgt
     2941 attgaacgat cattttgttt tagtttgtct tttcttgatt ggcggtgtgg gattatatta
     3001 ttctaattgg ttgaaaaccc tgcagccacc gtttgcaatt ggagggctca taatctctgt
     3061 attttggatg ctctgtttat tcgtgggtaa gatggctact tttgcagagg cagcagatat
     3121 tgttttttta cttcctaaag aaaaagaaat gcaaacctat ttggaacgag cttttcgcta
     3181 ttcttgcatt tttccgttga tctttttgat attggcatgc ggattggcca tgccattagt
     3241 ggtggttagt acggagcaat ccttttcgat gtttttcgtc tatctgatca tattatggag
     3301 tttgaaattc agtcatctgc aaatcaagag agcagaattg tttcgactag atcaacaagt
     3361 tattcgttat tggaaattag gctggttttt ttcttctctg atctttttgt ttctatcatt
     3421 ttattggcta atatgggcag gaatagcagg tgcattagta caggcaagtc tatattactg
     3481 gtttttgtgg aagcaacaag aaaaaggatt ggattgggag aaaatggtgc atgtagaaga
     3541 acataggttg catcagatct atcaatttat caatttattc acagatgttc ctgaaattac
     3601 ggcaaagatc aaaagaagac gctatttgga tcctatattg actcatatta aaagagaatc
     3661 aaaaaacact tatctttact tgtttgcaag aagattggca agaggttctg attttagtgg
     3721 tctttatttc cgattagtaa ttataggtgg gttattgatt gccggagtag tcgactttta
     3781 ttttagtctg attatcggtt tcttattttt atatttgatc ggtttccaac tgttaccttt
     3841 agcgaaccag ttccgctata tggttctcgt tcagctttat ccaatccata agaatcagaa
     3901 gaagcaggcg attcagtatc tactcacatg ggtattaatt atcgcgtcgg ttatttttgg
     3961 tttgattgct gcagtcgtat tagatgggca gcagcgatgg gttccgctag cggcttattt
     4021 gattgttact atgctctttg ttaaactata tgtaccaact cgtttgaaga agatgtcaga
     4081 ctgaaaacaa agctgtgtag ggtaaaaatg agaatgaaaa atagagcgtt gacagtaaga
     4141 atgctgttcg ttctattttt tttgagaaaa gctggttttt tgaatggcaa ttaaatgaaa
     4201 aagaacgtat atgtgcttga cgtgtagtgt tcaggcgact agaataataa gtactgcaaa
     4261 acaaagagta agaggacggg agaatgcatg atggagtttc agttagacca agagtggcgg
     4321 ttacgtccca tcaaaggcga cacaggaaaa acatacatag gcatgcgtga tgaggacaaa
     4381 gtctttatta aacgaaacac gactccgatg ttggctgctc tttcaaggga aggaattaca
     4441 ccaaaacttg tttggacaaa acgaacgggg aatggcgata ctttgacagc acaagagtgg
     4501 ctggaaggtc gcttgctgac tcctaatgag attggtcaaa gaaacgacgt gattgatgtt
     4561 ttgtaccatt tacatcattc aaattctctt aaaaccatgc tgacccgtat cggtggtcgt
     4621 gtcatgacgc cggagaagat gctgcaaaaa tatgaaaaag aattaccgga agaactccag
     4681 aaaaatcaat ttctgcaaat tgtccatcgt tttcttcgtg agcagatccc gtcaatagat
     4741 aaggaaaact tatcagtagt acacggagat gtaaaccatc gtaattggtt agtatgtaaa
     4801 aattatttat atctagtcga ttgggattca atcatgtttg cagaccctgc ggtggatatt
     4861 gcaacgattt taggaaatta tgtgccactg tctagctgga atcagtggct gatcagttat
     4921 ggcatacgcc cgactgacga aatgcttgaa aaatttcatt ggtatgcggc catgaatttg
     4981 ctccaagaaa tcaccagata ttgcaaacgt gaagatcatc gacggatgaa cgaagaaatt
     5041 ttacaattaa aacgaatttt cagtggctga ggtttggccc aaactctgcg gagatttggc
     5101 cagacctttt taattagagg aaaggaatgg agataaataa tgcgtgtccg aaatcgtccg
     5161 ggagcagcgg aaatgttagc ggctcacccg aattttgtaa ttagtgatcc aacattgtgg
     5221 aagggaaaat ggaatgaatt gtttgaaaac gatcatccaa tccacattga gattggtatg
     5281 gggaaaggcc agttcatcac tggaatggca aaagcccatc ctgaaatcaa ttatattgga
     5341 gtagaaatgc aggtcagtgt tgtttcgatt gcactagaca agttgatcga acaaccgctt
     5401 cctaacctga aacttctaca tgtggatgga tcagcgttga cagagtattt cgctgacagc
     5461 gaagtagatc aaatctatct gaatttttcc gatccatggc caaaaaaacg tcatgaaaaa
     5521 cggcgattga catataagac ttttcttgct gtagatgaac agattcttcg tccaaatgga
     5581 gaaatccatt tcaaaacaga taatcaagga ttgtttgagt attctctggc aagtttttct
     5641 caatatggca tgatcctcaa acaggtatgg ttagatttac atcaaagtca atttgaaggc
     5701 aatattatga cggagtatga agaaaaattt tcttcaaaag gtcaacgtat ttatcgagta
     5761 gaagctcaat ttcaagataa gaccaaaaag aaaagtctgt gaaactaact ttctgctttc
     5821 ataaaaacat taatatccat aaataaatcc cagaaaatga acattacaga tgtaatgtat
     5881 tttctgggat ttatttgatt taaaatgtat ttatttcatc ggtttttcca caaggtggaa
     5941 tcaattggaa gcgagaaaat gaaaataatc ctttgtcggt gatcttcaat tcgggtatga
     6001 cagataatgt taagaaagaa agtgtcaaaa aaggatcaaa ggttttagct gctcctagtt
     6061 gatgtgcttt agttattaat tcattcaact gttcatagac gatcgaaaaa ggttcctgtg
     6121 tcatgattcc gcctatagga agagaaacac aagctatttc ctgctgtttg ttgacaacgg
     6181 tcatcccgcc acctttttga atcaaaagat tagcagcata cagcatatcc tcgtcgtttg
     6241 tcccaacgac aactagatta tgcgagtcat gggcaatcgt tgtcgctatc gctccttgtt
     6301 tcagctgaaa tcctttcact atgcctaatc caatgtttcc cgtttgatgg tgacgctcga
     6361 tcaccgcaat ctttaattgg tctttttcga ttgaaggaat gaatcgttcg ccttgtcggt
     6421 ccactttttc taccaaatct ttagtcacta gactattcgg gataatttcg atcacatggg
     6481 cttgtgaaga agaaagaagc agctctaacg aatgtggatt taatggttga gcgtttagtt
     6541 ttggcagaca acacgtatat gaatttgaat gatcagcaaa taaatggtct tggagttgcc
     6601 cagattgaac aactaatgtt cccttataga aaacttcatg aattggcagc ttggtaagat
     6661 cgtctgttaa gaagaaatca gcttgatacc caggggcaat cgcacctagt gttttcaaat
     6721 caaaacattc tgctgcattc aatgttgcca tctgaatggc ttgtaatgga tcgattccaa
     6781 gtgcaatagc ttgacggacg atatgatcga ttgatccttc ttttactaaa tcattgatca
     6841 gtttatcatc ggtgacaaag aagcatcttc ttgaattttc aggtgtgatc gccggtagta
     6901 gcgatgccag atcttttgca acggtccctt ctcgaaccat taaatacata ccaagatcca
     6961 aacgttcttt agcttctttt gcagttgtag cttcatgatc cgtccgaata cctgctgcag
     7021 gataaacatt taactcttcc atgccgatac cagctgcatg cccgtcaatt tttttcttat
     7081 gctggtgcat caattggatc ttttctagga tgtctgtttc attatttgct actgcttgat
     7141 agttcatgac ttcagctaag cctaaaactt cgggtttttg taaaaaaggg gctaattttt
     7201 ttgcatttaa atttgcacca ttggtctcaa aaggggttac aggtacacaa gaaggtagca
     7261 taaaaaagat attcatgagt gtttgtcttg catcttctat cataaactca agacctgtac
     7321 tcccagcaat gtttcctatt tcatggggat ctgtcactac agtagtaact ccatgaagca
     7381 aggatacttt tgaaaattcg cttggtgata aaagtgagct ttcgatatgc acatggccat
     7441 cgatgaatcc tggcactaga tattgcccat tagcattatg tacgcttttt ccttcgtatt
     7501 caccgattcc tacgatcatt ccttcgcaga ttgctacatc tttttccatg atttctttcg
     7561 taaagacgtt gacgatctta gcatgcttga tcactaggtc agcaggcttt tttttacttg
     7621 ctacttcgat atatttcctt aatgttgttt ccaatggagt gcctcccaag ataaattttt
     7681 ataagtataa cataaattcg gtttttgcca atattttttt gtaaattcat tttatttgaa
     7741 acaaaaagat caaaaagacg tatgtttttt gtggagggga gaataatgga aataaaacga
     7801 ttatcggatc aaatgataag tgaaagtttt gccaaacatg cccaagacgt atatatattg
     7861 ccagcttcag aagtatttta catctatttt cgctatggaa aaaaacgaac attctggaag
     7921 aaagtctctt cggcagatgg acagcaactg atctcaagat ttaagtattt gggacagatg
     7981 gatatcggag aaaaaagaaa agctcagtta ggtgcattta cttattcttt agaaaaacag
     8041 gaagttcgtc tgcggttgtc ttctgtagga gattaccttc aacgagagag tttagtgatt
     8101 cggctattat acggtacttt tgaaacaccc catcgttgct ttagttctcg agattttgca
     8161 actattttac agaaaaccgt cagacgtgga ttataccttt tcagcggacc agtcggatca
     8221 ggcaagactt ctctaatgta tcggttggct ttggaagaaa aattgcaagt gattactatc
     8281 gaagatcctg tagagataga agaaccgttt tttctgcagc tacagatcaa tccgaaaata
     8341 gcacagactt atgatacact tttgagactc gcattacggc atcgtccaga cttgctaatc
     8401 attggcgaga taagagatga atcaactgca catgctgcgg taagagcagc actaacaggg
     8461 caccgggttt ttgcaaccgt acatgcacgc aatcttgaag gtacattggt cagaatccat
     8521 gaactgacaa agaaaaaaga agagctatct gaatgtttag ctggtgtcat ttatcaagaa
     8581 cttttggaag atgccaatca ccatccttct gttttatgga gttatcaatt tatggaggaa
     8641 gaatcagtaa aaaaatcatg ggaggaaagt tatgaagaag ctcaggaaaa tatttggcag
     8701 tcagaactat tctaaaggat taacaagaag ccagcaaaac cagtttgcac aaaccatggg
     8761 agaactgctt ttatctggtt ttagtctgca agaatccctt tatttattac tcacgatccg
     8821 ttcgtttcca cctgttattt tggcaaatat ccaacatcag ctaaaggaag ggcaagcgtt
     8881 tagtgaggta ctttcgcaaa taggatatgg agcagagcaa ctggtacaga tcgagttagc
     8941 tgaacgtcat gggaatctgc cgacaacttt gttggaaatt gccaaacagt ttcggcttgt
     9001 tgaaggattt cgcaatgatt tgaaaaagct gcttgcatat ccgatgctat tgctcacctt
     9061 tttgctaggt atcttgttta gtatgcggtt gttcgttttg cctcagctta ttggttcagg
     9121 aatggtcatg caagagcatt ggggtattca gctgatacaa gtctttcatt ggtatgttct
     9181 actattcatc ctagcggtta ctctattggc tttactagta aggtatcgtc tgatgaagtt
     9241 gtctcctgtt gatcagagtg aatggataag cagacaatta ttgattggac cgatatattc
     9301 cttgtatcaa tcggcttatt tgtcactaga gattggaaag ctttttcatg aaggtttcga
     9361 attaaggcaa atcatagtct gcttaaagga aacgaaacaa gaaagtctga ttcagcatct
     9421 tgcctctaag ttgattcaag gattggaggc aggtatgtca ttggctgagc agttccagaa
     9481 ttatcgtttt ctatcagaag aattcagtaa aattgtttta cagggagagg caaagggaag
     9541 tttaggtaaa gaactgattt tttacagcga atggacaaga aaacagcttt tcaaacgtct
     9601 tcatcactgg atgcatttta ttcagccaat catttttttg ggcgtagcgg ctttgatttt
     9661 attagtttat gccgctgttt tattacctgt ttacggaagc atagaggagg ttttgccatg
     9721 aaaaagagaa aaatatctta ttatagcggt tttacattga tcgaaatgct aatcgtctta
     9781 ctgatcatca gtgtattagt gttgttgttc gtccctaatt tgtcgcgtta taggaatcat
     9841 gtagaccaag aaagtcgtga agcgatcatc cagttggtag acacacaaaa agagctctat
     9901 gcgcttcaaa ataatggaag agttcctact gtggaggaat tgttgaatga aggctatatc
     9961 aaacgagaac atgcagaaat ctatcagcgg ccttgaaggt aaggttttag catcaggata
    10021 tacgttgttt gaatcactct tactgatttt attacttaca acgatccttt cttttccagt
    10081 tctcgcattt tctgcatgga agaatgaatt ggcagtctat caatttttcg gccaatttga
    10141 aaaacgaatc tattctacgc aaaaaatcgc tattgtgaat cagatccaaa caggctttta
    10201 ttggaatagt gaagagaatc aaatcatttt tcatgttccg aatccagata aaataaattg
    10261 ggaagtatta gatattcctg atgagattac cttgaaacgc catgcttcta ttacttttgc
    10321 tgctagaacc gggaatgaaa gttcactaaa agcttatcaa ttttattggg aagaaaaaga
    10381 gcggacgatt acgtatcaat tccagatagg gagtggtcgc tatacaaaaa gaattgaata
    10441 aaacaggatt tgttttgttg gagtccctta cagcgcttat gatcgcttct gtctgtgtct
    10501 acttagtgag ctttggccag ctgtttttgc ttcaggcaga aaagaaaagt ggggaggaac
    10561 tgctatttat tcgaatgatt tatgaagata tcaagacgca gcggatgtat cacaaaagac
    10621 cagagcgaag ggaagaaatg atcccatacg atatccaaat tgtcgataat aatggagtaa
    10681 tcgaaaaagt gtttgtcaat atggagaaag aagcgatcag tattgaaaaa acaagtaatt
    10741 ccagcattta cgctgattga atgtctaatt ggactgatga tattgagcag tctattgctc
    10801 acattcaatc ttttgattca gcaaaccatc caagtaagtg aatttttgca gcgtaaagat
    10861 caaaaagagt ggctaatatt tttagcacaa ttagaagaag aactcaagag cagtcacaat
    10921 gtctctgtga aaaatcagca actgcactac acgatcgagg ataaacagta tatcatcgaa
    10981 agatatcagg caatgatacg taaaagaaga acttccggcg gacatcaacc tatgttaaca
    11041 agtatcactg agcttcaatt attggaaaaa gaacacacaa tttcttttta tgtacacttt
    11101 gaaaatggag aagatggata tgccatatgg acaaaaaacg atcagtagaa aatgttataa
    11161 gggctctatt ttattttctg tcttgctttt atttctttta tgtacgtttt tattgatggc
    11221 agcattagaa agctaccgtt tgtcaatgga tcttaatata cgaatcaaaa attattatat
    11281 agcaaaaata atgaagcaga tggctttatc ggaacagtct acgcttgccg aaagttcgga
    11341 aggagttttt tactacacaa ctggatctgt aacatatcag tgggtacaac agtccctatt
    11401 cttagaggtg gaagtctcac cgtttatctt tcggttcaca gaagaagtaa aaaatgatac
    11461 agatactagt acagaatgat ggaatgttcg tgaaaacgcc ctcttttctg ttataacact
    11521 gtttttaata aaaaaatgtt tgccgtttca caaataaaaa acagtgaaaa cgattgaaat
    11581 tttgtcaatt gttcgataga attaaacagg tgaaaaaatt gttataatat atctagaaat
    11641 gaggtgcacc atgttaccag aaaaaatgga acaagggttt aatcaaaacc tggaagctat
    11701 tcagctttta cagaatgcat tgggaacttc ctttttagaa gcctatgtcg aaaatgcaga
    11761 aaatttaata gatgattatc aagtacgtgt ggtggatgga gtgccgacaa aagagacaac
    11821 gcaacggatc acagctcttt atgaagagtt gaagaaactt tctttcgagc cagaagaatg
    11881 gcgcagactg tctcaattac ttttattgaa gggcagccag acagaacatt tgcaggcgaa
    11941 ccatcaactc actcctgata gtatcggttt tttatttgtt tttcttattg aacaactata
    12001 cacaaataaa aaagcaccgg ttaagattct ggatattgcg gcaggaatgg gcaacttgtt
    12061 attaacagtt cttttaaacc tatcgaatgc ggggtaccaa acagaaggaa ttggtgtgga
    12121 tattgatgat acattgttgg ctgtcgcagc ttctacaagt gatttgacac aagcaaatgt
    12181 tcaatacttt catcaagacg ggttgcagga attattaata gatcctgtcg attttgcaat
    12241 cagcgatttg ccaatcggtt attatccgaa tgacgaaaaa gcaaaagagt ttttaacaag
    12301 taccgaagaa ggccatagtt atgcacatca tttattgcta gagcaatcaa tgaaatatgt
    12361 gaagccggat ggttttggtt tgtttttgat gccgagtgga tttttagaaa cggatcaaag
    12421 tgaagaaatc aaaaaatggt ttaaagaaga aggttatctt caaggaatga ttcaattgcc
    12481 agatgaattg ttccgtaata aacaatcgca aaaaagtatt ttgatcttac agaaaaaagg
    12541 accacaagca caacaagtaa aagaagtctt gcttgtgaaa cttgcttcat taaaagaacc
    12601 agagaaggtg acggaatttt tcaacgagtt taaaaactgg aaatcatctg ctctgtaatc
    12661 attcaacgag aaggagagtc atatgtctaa aacaattgca atcaatgctg ggagttcaag
    12721 tttgaaatgg caattatatc aaatgccaga agaaaccgta gttgcgaaag ggatcgtcga
    12781 acgtatcggt ttacaagatt ctatttttac catcaagtac ggcgataatc aaaaattcga
    12841 acaaatcatg gatatcgaaa accacgaagt agcagtaaaa atgctgttag atcagttgat
    12901 tgaactgcaa attttaagct cttatgatga aatcaccgga gtagggcacc gtgttgttca
    12961 cggaggagaa acttatgggg attcagtagt gatcgatgag aaagtaatgg accgcattgc
    13021 tgaattagca gaatttgctc ctttgcataa tcctgcaaac ttaatgggga tcaaagcgtt
    13081 taaaaagatt ttacctgaca ttttgagtgt ggctgtattc gatacatctt tccactcaac
    13141 aatgccaaag caaaattatt tatatagctt accggtgtct tattatgaag atttcaaagt
    13201 aagaaaatat ggcttccatg gcacaagtca tcgttatgtg tcggaacgtg cggcaaaatt
    13261 attgaacaaa ccgatcgaag aactaaaaat catcacttgc cacttaggaa atggtgtatc
    13321 gatcacagca gtcgatggag gtaaatccat ggatacttct atgggattca caccattagc
    13381 tggggtgaca atgggtacac gttcaggtga tatcgatcca gctgttctcc catatttaat
    13441 ggaaaaatta gagattgata ttgatgaaat gatcaatgtt ttgaacaaga aatctggtct
    13501 gctaggccta acaggattgt ctagtgatat gcgtgatctt gaaaagaact atgaaaaaga
    13561 acatattcgt ttagcttatg acattttcgt ggaccgcatc cgaaaataca tcggcggtta
    13621 tgtgacaacg atgaatggtg ttgatgcaat tgtctttact gctggtatcg gcgagaatga
    13681 cggacatgta cgtagtgaaa tcatcaaagg tatgtcttgg tttggttgtg aaatcgatcc
    13741 agcgttgaat gaattgcgtg gtgaagaagt agatatctct acaaaagaat caaaagtaag
    13801 agtattagtg atcccaacag atgaggaact aatgattgcc cgtgatgtgg agcgtctgcg
    13861 cggctaagcc aaagcattcg ttaggataaa agatgaaacg catgcaagag ggaattcttg
    13921 catgtgtttt ttttgcggag aagaccgcaa aatggtaatt taacttcaat ttcccttctt
    13981 ttttacagga tcttcttagg gaattttgct tggaaaagtg gtcaaaaggg aaaagactgg
    14041 cattttattt attgattttc aggaaaatcc caatatttta ttttcattca tctttataag
    14101 ggacattaca tatgtaaaaa gaaaaaacat ttttagatca gcctcgtggg ctgtcgactt
    14161 tattcttcgc tttatgttta gctagaaaac cgatccgaaa cattcattga tcgaataata
    14221 aaaacttgat tttccacctg tactttgcct gaatcgtgaa agcacaggta tgaaaatcaa
    14281 gttttttgtt gcgaaaaaac gtcctagtca atcagttggc cgagcggctg gcggctgatc
    14341 tgctcctgat caataacgag tgcttcttgg aagtgttgct gcaaaaagta gcggtcatgg
    14401 ctgactgcta ggagcgttcc ttgatagtct tgcagcattt cttcaatttc ttctcgagca
    14461 tagctgtcga ggtgattggt tggttcatcc aaaatcagga cgttgatttt ttctaaaaag
    14521 agtttcagca acgccaaacg gattttttca ccaccggata aatctttgat ccgtttaccc
    14581 acatcgcttt gataaaagcc acttttggct aattgccgtc gagcgacttc ttcgtttcca
    14641 gtcaatgtga tggcgtaagc cagcaaacgg ctatcgggat cggcaaactg cagctgctgc
    14701 ggcaaataac cgatttgcaa gctactacct aatttgatgg tgccaccgta aggcttttcc
    14761 tcaccgagta ataatttgag taaggttgat tttcctgcac cattgtcgcc gatgatggct
    14821 aaatgatctc cacgatagac tgcaaaactg ctgtctttga atagtacttg atcagcgtac
    14881 tgctggtgca agttttgaat gatgaaaact tctttgccag attttccacc attagacaag
    14941 ctttgcagcc gatttttcgg gggaggtggc ggtttgacga gtgtcagttt cgccaggcgg
    15001 cgttccagtt ctttggcttt tttgaagaag gactcattgt ctccttcatg tgcccactga
    15061 cgaaaacgac gaatcatgac tttcaaccgc tggacttctt tttgctgcag ttcgtaattt
    15121 tttgtcagct cagccaagcg agcttctttc aagactcgaa aacggctgta gtttccggga
    15181 tactcaatca atgcaccttc ttccagttcc catgtttttg tggtgacttg atcgagaaat
    15241 tgccgatcat gagaaacgac cagaaccgat ttttttgtat tctgtaaata gttttccaac
    15301 cagcgaatac tgttaagatc taaatgattg gtcggttcat cgagtaaaag taaatcggca
    15361 tcggctgcta aaattttggc taaagcgact ttaactcgtt cgcctccgct aagttctgtt
    15421 aaccgtgctt cctgacggtg tggaatccct aagccttgca agatcgctgc aatccggtct
    15481 tctaatacgt aaccgcctag ttcttcaaag cgatgctgca gctcaccgta acgggccaga
    15541 cacttctcta agtttgtatc tggttctgtc atcaatgctt cgtagcgact catttgctgt
    15601 ttgaggtcct gtatctcagc ggcagaatgg agcaagtagt ggttgacggt cgcttcggaa
    15661 actgtaagtt cttgttcgac cgtcccgatc tttaagcctt ttttgcgact gataacaccg
    15721 ctgtcgacac cttctctgcc ggtcaagatt tgtaaaaagg ttgatttgcc agttccgttg
    15781 ataccgacta aggcaatttt ttcaccagct tcaatttgac aggataaatc ctcaaataat
    15841 ggattcccgc cgattgtttt tttaatagaa attgcttgta tgatcatgat ctctctcctt
    15901 gagaccactc aaaaaggcca tggttatcca tggcctgtgt tatttaaact cagattatca
    15961 tgagaaaaat accttgagat ggccattgat tttacgtgaa ttcaactgtt ttggacaaac
    16021 ttgtcccgtc aacagcaact gtaaaaccaa ttgcatggct aaatgcgata tggtatcgtt
    16081 ggtaattttc attacttgtt attttagtca tgcgcatcat agtattttcc ctccttcgtg
    16141 tagttgtctt ttccagtgta cctgaaagtg tcagaaagtt caactagcgc tgacatttac
    16201 ttaatattta tcgatgtgta attctgtcag agagcttttt ttatctgact gagggtaatg
    16261 tctcgtggac cacatcattg tattctgagc taaaccagcg ttcaggcgaa atcatatggt
    16321 gtttgataaa aaactttgtc tacatgactt ccttttgcca tttgcttaat accgttgcat
    16381 taaacagctc ttctctttgg aaatctcacc aacatcgcgt tcaggagtat cgttgtggtt
    16441 gcaatggcaa gcggtagcct ttcatcaatt cttgaatgat ttaccgaatg tagcacttgc
    16501 cttctcgcgg tacccgaacc cgaaataaaa ttttagtttg gcgataaaat cggtatccgc
    16561 catcgatact tcatattttt tcgtatcaga ttcatcggaa acttccacat tgacgaacca
    16621 ataagctttg gcatgcttag gtcgttttaa accacaccag ggcgactaat cgccctggtg
    16681 ttcacttttt tttattttac ttgaactcat tgactgtttt ccctttattt taaacattat
    16741 aaagaccgat gagtgcttgc cgcgtctttt gtttttatca cggttttttg tgcaaaaaaa
    16801 cctgccgata gttggcaggt cgtctacgtg ttaagctttg gtgtggtctt tagctggcaa
    16861 ttggttttct aaatcagttc ggacaacaag cacatcgcaa gctgcgtttc taataacata
    16921 ttcagaaaca gaaccaataa ataaacgttc tacagcattc aatcctgtgg ctcctaacat
    16981 aatcaaatct acttgattat cttctgggat ttctctggca ataatttgtt ttggtgagcc
    17041 atattctacg acagaggtta cattattcaa gccagctttc ttcgcattgc tttcataatc
    17101 tgccaatgtt tgctttgcca tttcagtagc ttgttctgca agcattccat caaaggatga
    17161 aacggtttgg aaagaacgtg tgtcaatgac atgtgctaat agaagttctc cgttatttcg
    17221 gatagctaca ttgaccgcct ttttaaaagc taattcagct tcatcagaac catctacggc
    17281 aaccatgatt tttttgtatt gttgtaacat aagcattcac tcctcaagtc aatcgtattg
    17341 tactctttta ttgtagaaca ctttagggaa aaagagaatt atttactgct ttggttcagc
    17401 aatttttttt gcaaatcgga tactgaaggt ggcagaaaca ataattacga tcagtaaaaa
    17461 gaccaaggca ataaagcgtt gattgaaaaa agctgttaaa atacccatta cgcctaaaac
    17521 agcatatgca gctccgtaaa attgtaggaa tccttgattt ttatcatttt tgttgatcaa
    17581 tacaaaaaag atatcctgtt tattaaataa ataatagcta acgaataata atactaaagt
    17641 ggcgataatc attaaaatcc taatcatgaa caaatcctcc aaaataagca acaaatagaa
    17701 aaagcaattt gccaagagca aattgcttta ttctttaaaa gatggtaatc tccgttaatg
    17761 ccgttgtttg tcctgtagta ttgatcccgc gaattcaagc aggtgggtcc catggtcgta
    17821 gcttcgaact accaaatgat aaggcatgtt accagcagtg acacaagacg ggtcccaaga
    17881 tatcaataaa aatgttcggt caacacacaa agaggacaat tcgaacatca cagatttccc
    17941 acctctatag tagcatatga atttaaaaag agcaatgaga gagctcgcag aaacagaacc
    18001 gttttcttgt ctagtttttt ggaggatgct ttttccactc ttttatacgg taatactgtt
    18061 gacccagagc ctgttcatat ttccctgtat cttttggctg ataatactga gcattcttca
    18121 gtttatctgg taaatattgc tgattgaccc aagcctgatc aaaatgatga ggatactgat
    18181 aacccacgcc acggttgagt gatttcgcac ctttgtaatg gctatctctc aaatgatcag
    18241 gaacatctcc agctttgccc tctcgaatat cagcaagtgc tgcatctaat gccatgtagg
    18301 cagaattgga ttttggcgac aagcaaagat cgacgacgac gtctgctagt gggatacgtg
    18361 cctcaggcaa tcccaatttt tcagcagcta atacagcgtt cactgtacga gccgcagcag
    18421 caggattacc taaacctata tcttcataac cgataaccat cagacgccgg caaatacttg
    18481 ccagatcacc tgcctcaact aaacgggcga gataatgaag tgcagcatcc acatcacttc
    18541 cacgaatcga tttttgaaaa gccgaaataa cgtcataatg agcatctcca tttttgtcat
    18601 gggtaagagc ttttctttgg acacattctt caatgatcga cagtgtaaga tgaatccttc
    18661 cttctttatc tgaaggagtc gatagtgtag ccagttccag cccattcaat gcactccgca
    18721 aatcgccgtt cgttgcacgc gaaaggtgaa gaagagcatc ttcgtctaac tggattgctt
    18781 gttgtccaag gccgcgttct ttatccctta aagcatgttc gactgccaac tggatgtctt
    18841 gttctgttaa tggtttgact tcaaagatct gtgtacggct gcggatcgct ggattgatgg
    18901 taatataagg attctcagta gtcgctccga tcaagatgat tcggccactt tctaaatgag
    18961 gaagcagaaa atcctgtttt gttttatcta atcggtgtac ttcatccaat aagaggatca
    19021 ctgtaccact catttttgct tcttccgcta caacttgtag atcctttttt gaatctgtag
    19081 cggcgtttag catgcgaaat gcataattag tagagccggc gatcgcactt gcgatactcg
    19141 tttttccagt accaggaggt ccatatagaa tcatggaaga gagcatgcga gcatcgacca
    19201 tgcggcgtat gatttttcct tccccgacta gatgctgctg accaacgact tcatcgatcg
    19261 ttcgaggtct catgcgataa gccaatggct gttgcatagg attttcctcc ttcttgtttt
    19321 tacgtccctt aattatatca tatttagcaa gatgcctaga aagaacagcc ttctgttgtt
    19381 ttgttttcat agcttatcca actaatattg tgtatgataa agtaggtgag aaaaatgacg
    19441 gataaacaaa agatagatga atttttttgg aataaaacga cgccagaagt ggcacgggat
    19501 ttattgggga tgtatttgga gcatgacaca ccagaaggaa gattagctgg atatattgta
    19561 gacgcggaag catacctagg tccagaagac gaggccgccc atagctatgg tttaagaaga
    19621 acaccgcgtg tgcgggcaat gtatgaaaaa ccaggtacga tctaccttta cacgatgcat
    19681 actcatcgga ttttgaacat cattactcag ccagaaggga ttcctcaagg agtaatgata
    19741 cgtgcaatcg aacccgccgc aatgattgat caaatgagta aaaatcgtgg cggaaaaact
    19801 ggtccagata tcagcaacgg tccgggaaaa ttagtggaag cattagcaat tcctcaagaa
    19861 ctttatggtc agtctatagc agatagttcg ttacgcttag tttttgagaa aaaaaagaca
    19921 ccaaaaaaga tcaatgcttt gcctagaatc ggtatcccaa acaaaggtgt atggacagaa
    19981 aagccgttgc gttttgttgt gtcgggaaat ccttatatct ctttacaaag aaaaaaccaa
    20041 atcgagaaag attggggttg gaggaaagaa aatgaaaaag aaggatctat taacatattt
    20101 agatgaaata attgaaaaac aagtgacaga ctatgatgta gctttagatt gggatacaaa
    20161 aaatcatacg atcgagatcg ttgttcgctt gtttgcagaa aataaagcac aatttgagat
    20221 tgatgatgca gaagggatcg tttcgcaaga accaattatc gaatttgaag acggcgtact
    20281 tctttttaat cctcagaaat ctgtttttga tgaacgcaat tatctagcag taatccctta
    20341 tgaaggaaaa aaagggttgg caaaatcagt agctgattct ttagtggaat atctaaatga
    20401 agtattagct caagggcaaa gcgatttgct tgattttttg gatgaagacg atgacgaagc
    20461 tgtattcgag cttcactggg ataatcagca actagcagaa ttagtagaag taaaacagca
    20521 aaatgaagca gatacttatt taccgtaccc aagttattaa ggagtgacag atatgaaatg
    20581 gaatgaagta aaagtagaaa ctgaaagtga agcagtcgaa gcagttgcta atattttgat
    20641 ggaggcaggg gcgagtggtg tcgcgattga agattcttta gatgttgaac attttcagtc
    20701 ggatcaatat ggtgagattt tagataaaaa tgattttaca tcgctgaaag aaggcgcact
    20761 agtcatggct tattttccgg aaacgacttt cttaccggaa atcttgcctt ttatcaaaga
    20821 tcgtatcaca caattacctg agtttggtct agctattgga aaaaataaag taacagttag
    20881 tgaagtagag gaaagcaact gggcaacagc ctggaagaaa tactatcatc cggtgcgtat
    20941 tacgcgaatg ctgacggttg cgccaagctg ggaaaactac cagacagaag atcctctaga
    21001 aaaaattatc acactagatc ctggtatggc atttggcaca ggcactcatc ccacgacttc
    21061 cttgacattg acagcactag aaacagtctt gcgtggtggt gaaacattgc tagatgtggg
    21121 aactgggtcg ggcgtgctaa gtattgcagc aaaatatctt ggtgcaaaag atgtctatgc
    21181 ctttgattta gacgaagtag ccgttcgttc ggcgaaagaa aatatggata tgaatgaaat
    21241 agccaaagat gtccatgtat cagctaacga tcttttgaca ggaatcgaga ttgaaagtga
    21301 tgtaatcgtt gcgaacattt tagcagatat catcttattg atgatcccag atgcttggcg
    21361 tttactgaag caaacaggca cgctgatcgt ttcaggaatc attgaagaga agaagcaaat
    21421 ggttctggat gccatgttag aacaagggtt tatcgtggat caaattttcc agcaaaaaga
    21481 ctggtatgca attattttga aaaaaccaga ggtggaatag atgcagcggt attttttaaa
    21541 agaatcatat gaatcaaaag actattttgt tttagaaggg gaaaactatc accacattgt
    21601 acgggttatg aggatggaac ccgaggatga agtttttttg gcattcaatg atcaaatagc
    21661 cattgttgca gaaatcacgg agattactga agaggctgtt tatctgaaag aaattaaaaa
    21721 agaagaaaat caaaaggaac tgccaagttc gattaccata gcgagtggct atcctaaagg
    21781 ggaaaagctg gattggatcg ttcagaaatc cactgaatta ggagcgcaag cttttcttgg
    21841 tttcccggca tcagcctctg tcgttaaatg ggatgcaaaa aaacgtatga aacgacaaca
    21901 acggcttgaa aagattgctc aagaggcagc ggaacaatcc catcggcaga tcgtgccatc
    21961 agtagagtta ttggaaacga aacaagattt atttgagcgg ctagatgatt atgacgtggt
    22021 cttggttgct tatgaggaat cagcaaagca aggcgaaaaa tcaaatgcag ccaaaattct
    22081 tagtgaaaca aaaccagata gtcgaatctt agctgtcttt ggaccagaag gtggattatc
    22141 ccgcgatgaa gtcgattatt ttgtagaaca caaagggata ctttgtggat taggaccaag
    22201 aatccttcgt acagaaacag cacctcttta tttattaagt gctgctagct atcaatggga
    22261 attaatgaat ccttctttca gctaacgttc ttcattaata aaagagtatc gacagaaaga
    22321 attgaaaaaa tagtcaacaa tttgtataat agaaacaatt atttatacaa tggatacgaa
    22381 aaatcgtttg aaaaagcttt taaaaagcga acatttccgt aaaaaaacgt ataactgcac
    22441 ggattttggg acaattatca aacagatatt tgtcccgatt cccgttttac cgaaaaaaga
    22501 aagggggatc gattcctgtg acaaaagaag aaattttaac gggacccggt gtcataaagc
    22561 tcgttagtca atacatgggt cccgaacatg tagcatttgt tgagaaagca tgcgaatacg
    22621 cgactgctgc acatgacgga cagtttagaa aatcaggcga accctacatt attcatccta
    22681 tccaagtcgc aggtatatta gcagatttaa aaatggatcc ccatacagtg gctacaggct
    22741 tcttacatga tgttgttgaa gatacagaaa tcactttaga agatctgaga gaagaatttg
    22801 gcgatgacgt tgctatgtta gtggacggcg taaccaaatt agggaaaata aaatataaat
    22861 cccacgaaga gcagctagca gaaaaccacc gaaagatgct gctagcaatg gctcaagatt
    22921 tacgagttat catggtcaaa ttagctgata gactacataa catgcgtacg ttgaagcacc
    22981 tgcgagaaga taagcaaaga aggatcgctc aggaaacttt agaaatctat gcgcctcttg
    23041 cacatcgtct agggatcagc cggatcaaat gggaattaga agatacagca cttcgttatc
    23101 taaatccaaa acagtattac cgtatcgtcc acttgatgca gacgaagaga gaagaacgtg
    23161 aaaaatacgt aagtggtact gttgaagata ttcgaatagc gacagaagag ctggggattt
    23221 ttgcagaaat ctatggacgg ccaaaacaca tttattcaat ctatcgtaaa atgaaagatc
    23281 agaaaaagca gttcaacgaa atttatgact tgctagctat tcgagtgatc gtagattcaa
    23341 tcaaggattg ttatgctgta ttaggagcaa tccacacaaa atggaaacca atgcccggca
    23401 gatttaagga ttatattgcc atgccaaaag ctaatatgta ccaatctttg catactaccg
    23461 tgatcggtcc ggcaggaaat ccggtagaaa ttcaaatccg aacacaggaa atgcatgaaa
    23521 tcgctgaatt cggggttgct gcacactggg cctataaaga aggaaaaaat gaaaaagtag
    23581 aaccagatgg tatgacgaaa caattaagct ggttccatga gatactcgaa cttcaagacg
    23641 aaagctatga tgcttctgaa tttatggaag gcgtaaaagg agatatcttt agtgataaag
    23701 tctacgtctt cacaccaaaa ggagacgtta ctgagttacc aaaaggatcc ggaccattgg
    23761 actttgcata cagtatccat acagatatcg gtaacaaaac cactggtgca aaagtaaatg
    23821 gcaaaatggt gcagcttgat tacaaattga aaaacggaga tatcattgag atcatgactt
    23881 ctccaaattc atttggccca agtcgcgact ggttgaaatt agttgctact agcaaagcaa
    23941 gaaataagat caaacgtttc ttcaaagccc aagatcgaga agaaaatgtg atcaaaggcc
    24001 acgaatccgt ggtcaaatgt attacagatc taggatttac gcctaaagat attttgacga
    24061 agaacaaact gcaagaagca ctcgatcgtt ttaattatca aacagaagat gatctctatg
    24121 cagctgtagg gtatggagaa gttagcccct tgacgatggc caatcgtctg actgaaaaag
    24181 aacgtaaaga acaaaaaatc gagcagcaaa agcaagaagc agaagaaatc atgaatcagc
    24241 cgaaaaaaga acctgacaaa atgaaagtac gtcatgaagg tggcgttgtc attcaagggg
    24301 tagaaaactt actaattcgt atcagtcgct gctgtaatcc gattcctggt gatgatatcg
    24361 ttggttatat cactaaaggc agagggatat ccattcatcg tcgagattgt ccgaatgttc
    24421 agcctgacaa accaaatgta gcagaacgtt tgattgaagt cgaatgggaa gatacatcga
    24481 atacacgaaa agagtatgat gcagatttgg aaatttacgg ctataatcgt tcaggcttat
    24541 tgaatgatgt acttcaaaca gtcaatgcgc taacgaaaaa tctcaacagc gttgaagcac
    24601 ggacgaataa agataaaatg gcgacgatcc atttgacggt tggtatccag aatttatccc
    24661 acctaaagag tatcgtggat aaaatcaaag cagtacctga tgtctacagt gtacgccgga
    24721 cgaatggata ggaggacaga tatgagagca gttgttcaac gggtaagtaa agctgctgtg
    24781 tcagtcgatc agcaagaaat tggaaaaatt ggctacggcc tacttgtttt attaggcatt
    24841 catgaaaccg atacgcaaaa agatgtggac tatctggtaa aaaaaattgc acaaatgcgt
    24901 atttttgaag atgaacaagg aaaaatgaat attagtgtag aagatgtggg aggacaaatt
    24961 ttaagtatct ctcaattcac actgtttgcg gatacgaaaa aaggaaatcg accaagcttt
    25021 gtggaagccg cacgaccaga aacagctatt cctttatacg aatcattcaa tgaaggaatc
    25081 agaagtcgcg gcatcacagt cgctaccgga caattcggag cagatatgtc ggtttctctc
    25141 gtcaatgatg gacctgtaac gatcattatt gattctaaaa acaaataaag taatctggtt
    25201 ttcactagta aaaactatga aacacgtcgc ttaagttaga ctgataaact ctaacttaag
    25261 tggctttttt gtctgcaaaa agcgcttctt ttgtcagcag tcggaaaaaa agttaagctt
    25321 aaattataca agttattttt tataggaggt tgctgaagat gaaagaagta ccagtagtag
    25381 atatcaaaat cacagattta aaaaaattgt atcaaaaagg atacaacatg ttggaggagt
    25441 tacgccatga agcggacgct cccgtcgtca aagcaaagat tttcaataaa gaagctatca
    25501 cgatctacgg atcatcagca gcaaaagtat tttatgatcc aaggaatttc aaacgaaaag
    25561 gtgctatgcc aaaattagta ttgaagacac tttttggtca aggtggagta cagactttgg
    25621 atggagctgc tcatcaccat cggaaaaata tttttatgga tctaatgaca ccggaaagaa
    25681 tggaagatta tcatcgtatc ttagataaga atctgacaca agcacttgaa gcacagcatg
    25741 gtcaatttga actatttgat ttaagtaaga tggttttctt tacatctatt tgcgagtggg
    25801 caggtatcaa tttatcagcg atttctaaag atgaggtcga aaaattagca gaatatcaaa
    25861 tctctatgat cagcggaacg tttacttctc cgattgatca cataaaaggt gtcgaaaatc
    25921 gcaaaaaatc agaaaaatgg gcgcaaggct tgattgaaga agctcgacaa aatcctgtgg
    25981 ctggaaaaga aaatgtggca ttatatgctt ttgccaatgc cacagattta gatggtcagc
    26041 ttttacctct ggaagtggca gctgttgaat tattgaacat catccgtcca acggttgctc
    26101 taactgtgtg ggcggcattg atgggacatg cacttttcag tagaccagat ttgtatcagc
    26161 aattaaaaaa tgacttttcg acgttacaag atccgtttat tcaagaaatg agacgctatt
    26221 atcctttctt cccaatgcta cctgcgattt cgctgaaaga agtagaagta gatggttatc
    26281 gtataccaga aggcagttgg gttattttag atttatatgg aacagaccat gatgaacgaa
    26341 cagttgaagc gccagattcc tttatgatca aacggtatgt agggaaagca aaagatattt
    26401 cctataaaga agaatatgag atgattgcac aaggcggggg aaattttcga caaatgcatc
    26461 gctgtgcagg agaatggatc actcttcata gtctacgtgt gttttccgat cagctagtaa
    26521 ataagtttga gttctctgta cctgaacaag attggacgat tccattcaat cagtttccga
    26581 cttatccgaa tagccgagcg ctactttaca agaattagat tagcataaac ttatcgaaaa
    26641 aaaggaagat caaacagtgc tgatcttcct tttttcatat tactataaat tgataaggtc
    26701 aattgatcaa aatccacgaa atgcttcatc aatggtttga taatcttcat tggataaatg
    26761 caaatctaaa gcttggacat tgtcagatac ttgctctgga cgcttagctc caggtataac
    26821 tacagacact cgtggattct tcatgtacca agctaaaaca agttgcgcag gagtggcttg
    26881 gtatctttta gcaatggggc gaagtacatc gacagcggtt aaaatttctc tgaaacgttc
    26941 tccttggaaa tctggatttc tttttcgagg atctccttca ccaaattgtt tttcctcacc
    27001 aagttcatat tttcctgtca gcaaaccgga agcaagagga aaatagggaa caaaactgat
    27061 tttgtttttt tcaagataag ggaataattc tttttcagcc tgccgatgga tcaaactgta
    27121 tttatcttct actacatcga catatccatc tgcattcgct tctttcaatt gttctaacgt
    27181 gaagtttgac acaccgactg ctcggatttt tcctgcttct tttaattcgt gcaatgtagc
    27241 aactgattca ttttttggtg tgctttcatc cggaaagtgg atataaaaaa tgtcgatgta
    27301 atcagtttgc agacgtttta gtgcatcttc caccgattgt ttgagaaact caggagaatt
    27361 atcaaatgtt cgccctttat ttggtacatg agcagctttt gtagcgatga cgacacgtga
    27421 gcgatcatat tctttttcct tcagtacctc gccaatcagt tcttcggaac ggccgtttcc
    27481 gtaagcataa gcggtatcga tcaaattgat cccgctattc aaagctgtac gaactacttg
    27541 ttttcctgtc tcatcttcca aaccgtcaaa aagattatgt cctccaacgg catttgctcc
    27601 taatccaagc tgttctgcgt agacttgtga atgtccgatt cttatttctg ccatagctga
    27661 ttcctccttt ttgtctagtc taaaacaaaa taccaaaaag ctgaactaaa acgtttttgt
    27721 gaggggaaat agtcagatat ctcttgactt tgagactggt ttttagtagg ttataagtaa
    27781 tatcgaatgt caatgaaaga gcgagtaagt ttttgaaact ttacaagaga ggatcatcga
    27841 ggctgaaaat gatctaaagt ttcattagcc taaagacact ctggagatgg acgagtgaaa
    27901 agccgtcccg cgtacgagcg ttattcgtaa gaggaaagag aaatctttca aaattaggtg
    27961 gtaccgtgta tgaatttcat tcacccttgt tcatgatgaa caagggtgat ttttttattt
    28021 acatagctaa gaaagggaga gaaaactatg agtttccaaa gaccaaaagg aacaaatgat
    28081 attttgccag gcgaatcaga aaaatggcag tttgtcgaag aaacagcacg tttattattc
    28141 agagattatc agtataatga aatgcgtaca ccgatttttg aacactatga agtgatatcc
    28201 cgaagtgtag gggatacaac ggatatcgtt tcaaaagaga tgtatgattt ttatgacaaa
    28261 ggggaacgcc atgtgacgct tcgaccagaa ggtacagcac caatcgttcg ttcttttgtt
    28321 gagcataaat tgttcggccc agagtatgcg aaaccgttca aaacttttta tatgggacca
    28381 atgtttcgct acgaacgccc tcaaaaagga cggttacgtc aattccatca aatcggagta
    28441 gaagcatttg gatcagaaaa ccctgcaact gatgtggaga gcatggtgat ggcacttgat
    28501 ttcttcaaac agttggggat cacgcaaatc cggctagtca tcaattcatt aggtgataaa
    28561 gaaacgcgtc aagcctatcg tcaagcattg atcgactatc ttttaccttt tgaatcagag
    28621 ctgagtgaag actctaaacg ccgccttcat gaaaatccat tacgcgtatt ggacagtaaa
    28681 gacaaacgag atcaaaaatt tgtggcagaa gctccatcta tcttagatta tctaagcgag
    28741 cctgctcaaa aacattttga taccgttgtt tccatgctgg atgcattggc tgttccttat
    28801 gaaatcgaca gtaatatggt tcgcggacta gattattaca cacatacgat ttttgaaatc
    28861 atgagtgacg caccaaaaat gggggcacaa gcaacgattt gcgcaggcgg acgttatgac
    28921 aatctagtgg aagaactagg tggtccttca acacctggct tcggatttgc aatgggtatc
    28981 gagcgattac tcttgactat ggaggcagag gaagtagtta tccctgcatt taatgagttg
    29041 gatgcttacg tggtcgcact cggagacgaa acaaatatcg aagcattgaa agtggtccaa
    29101 gcgattcgaa actttggttt ttcagctgac cgcgacttca tgaaccgtaa agcaaaagcg
    29161 caatttaaga cagcagataa agcgaatgcc aaattagtat tggtgatcgg tgaagatgag
    29221 cttgctagtc aaacgattaa tgtcaaagca atggcaagcc gaaaagaaaa aagttttcca
    29281 ttagaagaga tatatgaaca ttttgatgaa gtctatgatg aaatgacttt attcgagtag
    29341 gaggaaaaaa ataaatggca caaagaacga tctattgcgg attagtttcc gaacaacaat
    29401 tagaacaaac agttacatta aaaggatggg tacaaaaaag acgagacctt gggggagtaa
    29461 tctttatcga cctacgtgat cgtgaaggaa tcgttcaaat cgtctttaat cctgaaatca
    29521 acaaagaagc atgggaaatc gcggataaat gtcgcagtga atacgtgatc gaagtgactg
    29581 gggaagttag aagacgcgga gaaaatgcag taaatcctaa aatggccact ggtgaattcg
    29641 aagtgattgc aagtgaaatc gtgattttga atcaagcaaa aaccccgcca tttcaaattg
    29701 aagatgatca agcaatcagt gacgaaatac gtttgaaata tcgttatttg gatctgcgtc
    29761 gtccaaaaat gactaaaaac atcaaattaa gaaatgcgac aacaaaagcg attcgtcatt
    29821 atttagatga taatgacttt ctcgatatcg aaacacctta cctagggaaa tctacaccag
    29881 aaggcgcaag agactaccta gttccttcac gtgtccatgc tggacatttc tatgcgttgc
    29941 cgcaatctcc tcaattgttt aaacaattgc tgatgaatgc tggattcgac cgttattatc
    30001 aaatcgttcg ttgtttccgc gatgaagatt tacgtggaga ccgtcagcct gaatttacgc
    30061 aagtagatat tgaaacaacg ttcttaacag cagaagaaat ccaaacttac acagagggat
    30121 tgattgcgaa ggtaatgaaa gaagttcgcg gtatcgaagt cacattgcca tttcctcgta
    30181 tgacctatga tgaagcaatg gctcgctacg gcagcgacaa accggataca cgttttgata
    30241 tggaattgat cgatctaagc gatacagtca aagaagtaga attcaaagtc ttccaaatgg
    30301 ctcttgaaaa cggcggtgtc gtgaaagcat tgaatgctaa aggcgctgca gatcgctact
    30361 cacgaaaaga catggatcag ttaggtcaat atgtaggaca attcggagct aaggggcttg
    30421 catggctgaa agtagaagaa gacggattga aaggtccaat tgctaaattc atgggagaag
    30481 caacagaagc aatcattaaa gcaacagatg caaaaccagg tgacttgctg atgtttggcg
    30541 cagataaaag tgaaatcgtc gctgctgcat taggtgctat ccggacacgt ttaggaaaag
    30601 aattaggact aattgatgaa tcgaaattca atttcttatg ggtaactgat tggccacaat
    30661 ttgaattttc agaagaagaa ggtcgatatg tctctgctca ccatccattc acgatgccaa
    30721 aagaagaaga tatcccgtta ttggctgaag accctgcaaa agtctatgca gaagcttatg
    30781 atatcgtctt gaacggttat gaattaggcg gaggttctat ccgtattcat actcgtgagc
    30841 ttcaagaaaa agtactagaa actctaggct ttacaaaaga acaaatggaa gaacaatttg
    30901 gtttcttgtt aacagcttta gattacggat tccctccaca tggcggtatt gcattaggat
    30961 tggaccgttt tgcaatgtta ttagccggag aagataatat tcgtgaagtc attgcctttc
    31021 ctaaaaacgg aaaagcagca gatgtgatga gtgatgcacc aagtatggtt tcgcctttac
    31081 aattgttcga attgaatatt gatgtaacga atatagaaga atagccgata aaaaggtaaa
    31141 aaggatttgt cgaatacgct tcttttgaca accttgattt aattgaaaaa cactttataa
    31201 tgaagccata acatataaag cggggctttt ccatgaaaaa aacattcgaa aattgggctt
    31261 atcatgatta cacgaccaag ctttcggtat ctatcgttta tgcgttgatc gcatcagtcg
    31321 cattgaattt cttttatcag ccaggacata tttattcaag tggtattact ggtctagctc
    31381 agattttgac gactttgtca aaatcactga taggattcga agtaccggta tcaacaactt
    31441 tgtatttact gaatatacct ttgtttgtat tggcgtggtt aaaaatcggg aagaagttta
    31501 caattttcac ttttatcaca gtcactttga cctccctttg tatccatatt ttaccggaaa
    31561 cagtcctatc taccgatcca atcatctgtg cgttgtttgg gggggctgtg atgggactag
    31621 gtattgggct agcgttgaga aacggcctat catcgggcgg tctagatatc gtgagtatca
    31681 cgattcgtaa aaaaacagga aagtcgattg gttctatttc gatctacttc aatgcattga
    31741 tcgtttttgt tgccggaatt ctatttggct ggcaatatat gttttacagt acgttgtcta
    31801 ttttcgtcag tggaaaagta actgatgtcg tttatactaa acaaaagaag atgcaggtaa
    31861 tgatcgtaac gaaacatcca gaaaaagtag taaatgaaat ccaaaactgt atgcgtagag
    31921 ggatcacgat catcaatgaa gcagaaggtg cttataagca tgataagcag acagtattga
    31981 tgacgatcgt tacacgatac gaactgcctc tacttagaga agcaatgaaa gcttctgatc
    32041 cacaagcttt tgtaagtatt gcagaaaacg tccagatact tggtcgtttc tatgaggatg
    32101 gcatttagat cgagtttgac aaaaattcat gaaatactgc tgtagacaga cgttcgtttg
    32161 tctgcagttt ttttctacca tagtagaatc agcaaagaaa aactgctttt ttatttgaaa
    32221 ttttcttaaa gttgaaaaaa agcatgttta tttttttaaa tttatgggat gatagaaaaa
    32281 aatagtagag aaaggacacg attctacttg ctattttcac ttaatacccg tatattacga
    32341 atgaaaaaag tttatgaaaa gttggaatat tgaatggaaa aacaaaaaag gttaaaaaac
    32401 agccgctaca tcactgggtt tgatgggctc cgttcattag ctgtggtggg tgttatcttc
    32461 tatcaccttc tgccaactag tatgaaaggc ggctatttgg gcgttccgat ttttttcgtc
    32521 gtatctggtt atttgatcac ggatctgctg cggcaggaat gggaacaaaa tggaaaaatc
    32581 aatatttggc aattttatgt cagaagaatg aagagactct atccggcact tgtttttatg
    32641 cttgtgacag cttcggctta tatcactttg tttcagcgag ggctgttaaa caatcttcga
    32701 ggagttgtgg tcagcagctt actgtatgtc aacaactggt ggcaaatcaa taacggttta
    32761 tcttatttcg atcgatttgc taatgagtca ccgtttacgc atatctggtc tttagcagta
    32821 gaaggtcaaa actatttggt ttggccgatc ttgtttgtat tgttgatggt atttgttcgt
    32881 aaaagaaaat ggatctttta tactgtgtta ggtacatcct tcctctccgc catcttgatg
    32941 atgatcttgt atacaccagg aggcgatcca acccgtgttt attacggtac cgacactcgt
    33001 ctgttttcta tatggttggg aagtgcgctt gcgtttgttt ggccaagtac gcgtttaaag
    33061 aaaaacatcc cgaaacaagc gaaaaatatt ctgaatatcg ctggaggaat ctcgcttttg
    33121 gcactgattc tttgcttctt ctttttagat gatcattaca atttcatcta ttacggagga
    33181 atgctcctag tgagtctgtt atgcgtagtg ctagttgcag tgacagcaca ccccggagca
    33241 agtttgaatc gatggctgac aaatccagta tttacttgga ttggcaaacg aagctatggc
    33301 atttatctgt atcagtttcc agttatgatt ttttatgaag caaaggtgac tgatattgcc
    33361 gatcatgttt tgttgcatac tttagtggaa atcacattga ttctaatcat cagtgaactt
    33421 tcttatcgat atgtggagcg tccgctagca cgttttgatt atggtcgtac attccaagtg
    33481 atgaaaggat ggttcagcaa accgatcatc agtaagagca aaccatgggt tgttccaggc
    33541 acattacttg tagcaattgc actcgttggt ttcgttacgg caccgaaaaa cgctgtaaca
    33601 gccgatcaaa aacaactgca ggcaaaaatc gctgaaagta aaaaattagc agaagaaacc
    33661 agaaacaatt cagaaaacac cactagttcg gttgaccaag cgatccttga taagtacgag
    33721 ttgtccaagg cacaagggaa taaggcacaa aaattagaac taacggcatt tggtgattca
    33781 gttatgttgg atgctgcaac ggatctacaa gaactttatc caaaagtagt ggtagatgga
    33841 gatgtcggac gacaattata tgccagtgta ccgtacattg aaaaattaaa ggaacaagat
    33901 ttgttgaaag acactgttct gattggacta ggaacgaatg gctcattctc ggaagctcag
    33961 tttgatagtg tgatggatgc aatcggtgat cgaaaagtat actgggtcaa tgtccgagta
    34021 ccgacgaagc gctggcaaaa tgacgtgaat gcgatgcttg aacagatgac gaaaaaatac
    34081 gacaatatga cgctgattga ttggtacagc tacagtaatg accatagtga ctggttttat
    34141 gatgatcaag tccatccaaa tcctgaaggg atgacacatt acattcactt agtcagcgaa
    34201 gccttgctag gaggaagttc aagtacgaac agcagtgaga caaacgtgac agaatgatag
    34261 ctacaaaaaa cttcaggcaa aaacatttgt ttttgcctga agttttttta tatttaccgt
    34321 gtttttaaca ttcatataga ttcaggaagc aaaacggttg agtgtgtctg taaataacgt
    34381 tctttttgtt ttcgggtgag ttttttgaat gcagcttctg cttttgttgc ttcgcttctt
    34441 gaagaaaata cttctgcatg gatcatctta acaggtcttc ttttttgaag acgagtatat
    34501 ttagcaccag taccattgtt atgttcctct aatcgtctag aaagatcggt tgtatatcca
    34561 ccataaaatg tctgatcttt acagaataat acgtaaaaat aatgttcatt tgccatagag
    34621 catctcacga atctctgcag tgtattctcc atccatattg taagtgatca atggtggagc
    34681 gactttgaat ccgtctaatt tgccttcttt gattccttca actaacaaaa tatttgcctc
    34741 ttttccaggt tttgggtaaa caaattggat ccttttaggt gcaattcgat agtcttccat
    34801 gacctgaatg atttctaaga accgatcagg acgatggacc atagtaaagc gtccatttgt
    34861 ttttagtagc ttacttgaaa tatcgatcac ttcttctaac gtcgtatgaa tctcatgacg
    34921 ggcgattgcc aggtgtttat tagggttcgt tttatttgtt ggcagccctt tgaaataagg
    34981 tggattacag acgactaagt cacaagaatt atgtcgaacg acagataagc tgtctttcag
    35041 atcgatggtg tggacagtca tttgttcatt taaatggtta agcgcgatac tacgtcttgc
    35101 catgtctgct agacgaggtt gaagttcgat agcgtcgata gccgcctggg ttcgtttact
    35161 taaaaaaagt ccaactgcgc catttcctgc acaaaggtca acaatcttcc cttttttagg
    35221 tacacgggaa aagtgagcaa gcagcacagc atctaaagaa aaagaaaaca ctttggaact
    35281 ttggataatc ttgatatctt ctgcgtataa ttggtcgatt cgttcgtctt cgtgaagcat
    35341 ggattccctc caaaatgaaa aaaatagtcg tctgtattat actattttta ttaaaaagaa
    35401 ccaaggtggc ttgtcaaacc tctatttttt atgcatacta tgaccataag aagggaaagg
    35461 aagagactat gttttatcga tttatgcgcg gggtagttcg agtactatta gccttagtta
    35521 atggaaatgc ccgctatgag aataaagaag ttttaccgca agatgagaat tacatactag
    35581 ttgctcccca tcgtacttgg tgggacccat tatatctggc ggtagctgca gcacccaagg
    35641 aattcagctt tatggcaaaa gaagaactgt tcaaaaatcc agttctgcga tttatattaa
    35701 ctcgatccaa tgcttttcca gttaaaagag aaaatccagg accaagcgtc attaaaacac
    35761 ctgtaaagat tttgaaatcg acaaatttga gtttgatcat gtttcccagt gggacaagac
    35821 atgctacaga acttaaaggc ggaatggcat tgatttccag aatgtctaaa gcgaaaatcg
    35881 tgccagctgt atatcaaggt ccgttgacac ttggggatct gttcaagcgt cagagagtga
    35941 cagtgcgttt cggcgaacca atcgacttat cagatatcaa gaaaacggat aaagaaggta
    36001 tggcggagat cgaacgacga acacaagcag cttttgacaa attggacaaa gaagtgaacc
    36061 ctgatttcaa atatgaagtc aaaaaaaaat aaaatgataa gtttatgcgt gggcggtctt
    36121 ttgaatctac tgtcctgttc agttcagact ttttcaatga aaacgaggga gagacgtcaa
    36181 gtctgccctc tttttgcgtt ccaatatgta aaatagaaga caggtgtcat gtaatggatc
    36241 aaagtgttat aataattaga aggacatttt ttgaggtgat tgaatgcgtt ttttacatac
    36301 agccgattgg catatcggaa aaaaactaca aggttatgac ttgattgaag accagcgtca
    36361 tgtactatct tctatactag atatcgcgaa agaagaaaaa gtagaggcaa tcgtgattgc
    36421 cggagactta tatgatagga gtgtcccagc tgttgatgcc gtagaattat acaatgaatt
    36481 gatggtcgat tggaatttaa aggaaaagat cccgattttt gctatatcag gaaatcacga
    36541 tagcagtacg agactttcag cgggtacgcc atggttcagt tattcgaact attttttgcg
    36601 aacacagcta gcagaagcat ttgaaccagt cgagtttaaa aacgtacaat ttttctttct
    36661 tccttacttt gaaccaattg cagcgagatt atattttgaa gatgacaata tccgaacgat
    36721 caagcaagcg gtagccaagg tgatagataa aatgcaggag ctatttgatt cagaaaaaaa
    36781 acaagtattg attagccatt tttttgtaat gggaagtgta aaatctgatt cggaaacgaa
    36841 aatcgaagta ggcgggttgg attcgatccc tggtgaacta cttacaccgt ttgattatgt
    36901 agcactagga catttgcata atcaagctgc tttgagacag gataatgctc gctatagcgg
    36961 ttccccattg aaattctctt tatctgagat caaccaaaaa aaaggcgtat ggatcgttga
    37021 cctgacaaca ggcctggagt tgaattttag ggagatcgaa cccctgcatg atattgaaga
    37081 aataacagcg agtttcaaag aattgctgga tcctgccttt tatcagtccg tcaatcggga
    37141 gaattacatc tatatctatc tcactgatag aacgatcatc ccgaatatga tgaaccagct
    37201 tcggcaaata tatcctcgta ttttggggat tgaacgggca aacggtcgag cagaacaaac
    37261 aaagcgcaaa cgaaaagtac tagaaatgaa agatcctcaa aaattagcag aagcattttt
    37321 tagtgaaatg acggaagagc agctaactgc aaaccaagaa aaatggctcg gagaaacgtt
    37381 gcaagaaatc atggaaatgg aatgatcaag aatgaaaccg aaaaaattac aacttaaaaa
    37441 ttttggtcct tttatcaatg agaccgtaga tttttcacgt ttgacagaag caccgctttt
    37501 tttgatcagc gggaagacag gagctggcaa aacgacgatt tttgatggga tcaccttcgc
    37561 actttttggt gaaacttccg gaagacttcg ctctggaaaa gagatgcgtt ctttgtttgc
    37621 tacaccagac gaagaaacaa gcgtgacttt ttcttttgaa catcagcaga tgagctatga
    37681 aatcgaacga aaaccagaac aagttttagc aaaacgaaaa ggaaacggaa caaaaaaaca
    37741 ggcagccaaa gtaacgttga ccatttttga tgggaaagga aaagaaataa aacaaatcac
    37801 aaaacgtaca gaagcagatc agttgatcag agaactgatg aatctcgatg cgaaacagtt
    37861 ttctcaaatc gttttactac cccaaggaga attccgtaat tttttggttt catcaagcag
    37921 tgataaagag actgttttac gtcatttatt cggtactcaa tttttccaac agttcaatga
    37981 gcgattaaaa gaaaaagcaa aacaacagca acaaaaattg gatcatttag agcaagagct
    38041 gcatcttctg caaaaaaggt ttgttttagt caaagaagag gaaccagcag cagggtttga
    38101 agccgtttta gcccaatgga caaaccatca ggcaatactc gaaaaacaaa tcacggaaga
    38161 aaaagaagct ctaacggaac ttcaggaaaa acaaaagcaa ctagaaaatt cctactatcg
    38221 ttttgaaagc ttgaaaaaaa gttatgaaga aaaaacgata ttaataaaca agcaaaagga
    38281 attagctgct caaaaagacg aaatagagga aaagaaacga tggattgggt attatgaatt
    38341 tacgcaacga ttagtcgaac caatccagcg cctacaagaa ataaaagttg aaaaaataga
    38401 actgtcacaa aaaagtagtg agcaaacaaa gaaactagcg atgactacgc agaattatga
    38461 agagtggcaa aaaaatgaag caaaccgcgt acgccgacaa acaaaaatca aagaagctgc
    38521 tcaaaaactg caaaacaaac agatgatggt gccaattgtc gaatccttac gtagtaaaaa
    38581 acaagacgca gaacaattaa caatttcatt ggataaagaa gaattgaatg ccgagaagct
    38641 gtatcaaaat ctcgctatct atcaggaaag aactcgaatt cttcaagaaa atttactgga
    38701 tcaggaaaaa ctgcaagaag atcgtcttgc ttttggacag ttaaaatatc tagatgaaaa
    38761 gcagcaagaa atagaaaaac aaaaagcaaa agacactggg tcgctcgcac agataagaga
    38821 agaactcaaa aaagcaactg aagaaataac aaaaaatagt cagctattga aagaacagac
    38881 agaacaagca gaacagctga aaagccaatg ggcaaagcag caaattgctc gtcttcagct
    38941 ttttttgcgt ccaggggagc catgtcttgt ttgtggatca acggaacacc ccatacaaca
    39001 attaaaacac gaacagccaa ataaggaaga tatcttaaaa gtagaacaac aactggcaga
    39061 agctgagtct gctgtagaaa gaactcagca aatcattgcc aaaagtgaag cacatgaaaa
    39121 taatcttcaa caacaattat cagatttaga acaaaaaatc atggcaacga atatacaatt
    39181 ggctgaacaa gaagaacact tgcaacaggc tttgaaaaag gcatttcctg agataacaga
    39241 acaaaaaaca aaaacaactc ttgtagtaat cgaacaatct ttagatagac gaaaaagcaa
    39301 gatggccaat gcaagaatag agctggaaga aataagacaa caagcagaaa agctcgaaca
    39361 acaattagct gtctacaaac agcagcagac agaaaaaaat aatcagctcc agcaactgca
    39421 aggagaaatc aaaagtttag aacaacaatt gcaagatacg gatagtaact gtttagaaga
    39481 ggagatacga cgacttgaaa aacagttaga agaagactcg aatcaactag ctgttgagca
    39541 gaaagcagga gtgcaattgt tgcaagatct tcatacgctt cggacacagc ttgatcttca
    39601 acatgaacaa atagaaaaaa tagatgaaaa aatagaaaag gaacaagaaa gaatagctaa
    39661 aaagttagaa agccagtcgt atttttcaac aattgaagaa atctgtgcat ttgcacaaca
    39721 gcaagcacaa tataaagaaa accaacgtac agttacttct tatcatgaag agcaattagt
    39781 gacagctgac cggctgcgtc aattagaaaa tatccagcta actgagacat tcccagatac
    39841 agaagagatc agccaacagt tagaagaaat aagacgtatg atcgaaataa agcaagaaaa
    39901 gatatacggt ttacaagaac aaaaaattag taatcaaaaa atctacgatg aatgtctttc
    39961 gatttatcac acaagccaga cgaaattaga tgagcttacg caattacagc agctttcgca
    40021 aacattcaac ggagaaaatc ctaagaagac tagtttggaa cggtatgtgc ttcaggttta
    40081 tcttcaagaa gtgttacaag tagcaaatga tcatctccaa cgtttaacga aaaatcgtta
    40141 tcaatttgag ctagcggata ccattggcag ttatcgaggg aaaaccggat tagaaatcaa
    40201 tatctatgat gatcaggcag gtatgtctcg aagtgcgcat accctgtctg gcggtgagag
    40261 ttttattgca gcactttctt tagcattagc tcttgccgat gtgatccaga cacaagctgg
    40321 tggtgtagca atcgatgcat tgtttattga tgaaggattt gggtcattag atgaagaagc
    40381 tttggaaatg gcaatggaag ctttagaaac gatcgaaagt gaaggaagaa tgatcggcat
    40441 cattagtcat gtaagagaat tgaaagaacg agtattacaa caaataagaa ttgatacaga
    40501 aggtagcgga caaagcaaaa tacgctatca acttggataa aaagggagat tggtaactat
    40561 gaaatggagt attccagaaa aagtcattgc acgtggacgt caatacttgc aagaaggccg
    40621 agtgttatcg gttgtccctg accaagaaga aaacgtgtgg catgcagaag tcttgggcag
    40681 tgaattgtat atggttgatt tggatggtac agcaaaagag atagattact gccaatgtcc
    40741 ctattgggag gaacataaat attgtaagca tacagttgct gtggaacttt atttacggtc
    40801 gaaaaatcta tctcgtatca tgaaaaaaga ccagccaatg ctagaaccgg taaaagcaac
    40861 aagtgaggga gaaatcattg caaaaggttt ttctcgtcta aaaacacaaa acaatcctca
    40921 agcggtgcaa cctttgatta ttgaatacca agtagaaacg attgagacta atcagtatca
    40981 cccagagctt tctattttgg cactttactt gcgtatcggt tcattggcat caccgaaaaa
    41041 aacatatatc gtaaaaaaca tctatgattt tttacaagct tacacagaaa aagaaactta
    41101 cactgttaat aaacagtacc agtttcgtct agataaacgg gcgtttcaaa aagaagatca
    41161 aaaaatactc acgcatcttg caggaagtgc tcagacgcat caattattag gtacgaacgg
    41221 cttacaagtc aaaggaaaac tagataaaag atatctgctt ctcccagtag aacaagcccg
    41281 cttcctgtta gagcagatga atcagactac acgtttgcat ctgcagataa atgaacaaaa
    41341 cgatcaagga atacaatttt ccgatcaaaa caaaccttta tcattcaaag tggaaaaatt
    41401 tggagaagat tatttgctgc aagcattgaa tgattttgat tttttcttta tgcattatca
    41461 gtggggagtt atccaaggga cattttattc tctgacgaaa tatcaacaaa cggtttatca
    41521 aacttggaaa caattgattc gccgattgga gaaaccagaa gtagtgttca agaaaaaaga
    41581 attaccgtct ttgtttaaag agattatccc tctgctgtca gagatcggcg acgtagctat
    41641 tgcccctgca gtagaagagg aagtagcaga tatccccgtc gaatttattt tctttttccg
    41701 aaaagcaaaa ggaaaaatcc aagctcgagt cgatttcgtc tatggtgaag tcatctattc
    41761 gactgacccg aagcatgagg tctcgacaga acaggctttt caggtgttac gtgacacagt
    41821 agaagagaaa cgagtacttg atctattcag aatgtatcgc taccagaaaa atgaaactgg
    41881 ttatgaacgt ccattgccaa gcggggaaga actatatgcc tttttccgca cagaactatc
    41941 tgtcttccgt cagtttggtg aagtcagact tggaagaaaa ttgcgagagt tgtatttaga
    42001 tgcgcataaa catcaaccga aaatcgaagt aaccgagacc ggttcatggc tcgatattcg
    42061 attcgatgtg acagggatag aggagcaaga aatcgatcat gtcctgcaaa gcttgctgag
    42121 aaatgatgct ttttatacat tagaaaatgg tcaggttctt tcttttgatt cagaagaatt
    42181 tcaacaaaca agccaagtgt tgcaacaatt acgggaatcg atacgtacag aagaaggaac
    42241 gatccatgta cctaaaaatc aaggattgat cattcaaaat caattggaaa aaagcaatgc
    42301 tacttttagt gaatcgttcc aaacgatggt acaagatctc attcatccag aaagatacca
    42361 ggcacagctt cccaaaggat tgaatgcaac gatgagagat tatcaaaagc aaggttttcg
    42421 atggttgaag atgttaggtc attatcagtt tggcggtatt ttagccgatg aaatgggact
    42481 tgggaaaaca ctacagacaa ttgccttttt actttcggaa aaagaagaac ggaaaagctt
    42541 ctctgctttg atcgtagcgc ctgcgagctt aatttacaac tggcaggcag aggtgagaaa
    42601 attcgcccct tctttaagta tccaagtaat caatgggaat aaaaaagaac gtgaagaatt
    42661 actggctaaa gacacagata tccgagtgac ttcttatgcc agcttaagac aggatctagc
    42721 taattatcag tcgcaaaaaa ttgattattt gattcttgat gaggcacaaa tggtcaaaaa
    42781 tagcagtacg aaaactgcac aagcattgag agaattagct gtacctcagc ggtttgcact
    42841 gagtgggaca ccaatcgaga ataatttaga agaattgtgg tcattgtttg caacgattat
    42901 gccaggattt ttcccgacta aaacaaaatt cagggaactt gccgcagaag aaattgcgca
    42961 aatgattcga ccgtttattt tacggcgaga taaacaaact gtgttgaaag acttgccgga
    43021 aaaaacagag atgaatctat attctgcact tacagaagaa caaaaaactg tttatttagc
    43081 ttacttgcgt caaatgcgtg aagaaatcac atcaatggat tctgaagcat tcaagaaaaa
    43141 ccggatcggg atacttgctg gtctgacaag actgcgacaa atttgctgcg atcctcgtct
    43201 gtttattgaa gattaccaag gcggttccgg caagctcgaa caagtaaaag atctactggt
    43261 agctgccaaa gagaacaaac gaagagtttt gctgttttct caatttacag ggatgctgac
    43321 tatcttgcaa gaagagttag ccgagctagg gatctcgaca ttttatttaa gaggcagtac
    43381 gaaaccacaa gatcggttaa gtatggtaga cgcatttaat gctggcgaaa aagatgtttt
    43441 tctgatttcc ttaaaggctg ggggaactgg attgaatctc acgggagcag atacggtcat
    43501 cttgtatgac ctttggtgga atcctgccat tgaagaacaa gctgctggac gtgcacatcg
    43561 gatcggacaa aaaaatgtgg tagaagtgtg gcgtatgatc tctgaaggaa cgattgagga
    43621 acgcatggat tcactccagc aagaaaaacg cgaactgttc caaaaagtga tccaaggtaa
    43681 tgaagaacag ttgacgaaac tgactgaaga agacatccgc atgattttaa gtgtaggaga
    43741 aatcgaggag taagagactc gaaaaagcgt tcagtaatgg gtaagaaagc ctttctaaag
    43801 aagcgattgt ttatttgatt taaaaaatag caaggaggct gggattccat tttatcccag
    43861 cttccttgct atcatttttt tatttctatt tttaaaggtt atacaaagat tgtcttcatt
    43921 gtctctaaca gctgatcaaa agaagaaatc tcgatatcgc aaattggagt ctgttcaatt
    43981 ttgcgttctc gatggttgaa ccaaagcgct tgccagttag cactttttgc acctagaaca
    44041 tcattgtcgt aattatcgcc aacgtataag gtctcttctg gaagcatatg aaattctttc
    44101 tcagccagct gaaaaatctc tttttctggt ttttggaaac ctgttgcttg agaaatgagc
    44161 atattgcggg aaggaatcca attgttcaat tgaagttgct tcagtttttt tgtctgatgg
    44221 tcagtcggcc catttgtgat aattcctaat gggacattct tttccttcaa gaaatcgagg
    44281 gtttttttga cctcctcatg caagcagata ttatcaagtt cttcctcgta aattttttga
    44341 aataagaggc catcttcttc cgtaatatga gggtagtcta aatcttttag tgattggctg
    44401 atccgatgtg ctcgcatata ttctagtgtc caatctcctg ccattacttt tgggaagttt
    44461 tcatcactat gatggcggaa ccggatatat agtggatgca tatctgcatc tgaaacaagc
    44521 ggaacgactc ttttgaccgc gtttcgaaat ggctgctgct gatcatacat cgtatcatca
    44581 acatcaaaca caactgattt aatcaaaatt ttacacgcct ttcggttttc attgattttt
    44641 tcacaaatcc ttgactattg taacagaaaa aattgtctaa aaaagctgat aaataaaggt
    44701 ttttcttact atatttctat ttccttgaca gaataaggtt tttatatttc agtttttttc
    44761 actaaaaatc gtgaaacacg tcaataagtt tgatgaacaa attaatcatt atggtagaag
    44821 tcgattgatt tattttaatg caatctctgt ggaattttcg aaagatttat ttttttaata
    44881 cag
//
LOCUS       DAZKOU010000014        40984 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000014 DAZKOU010000000
VERSION     DAZKOU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.014"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACG827_000716"
     CDS             complement(38..1189)
                     /locus_tag="ACG827_000716"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0482878.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACG827_000717"
     CDS             complement(1202..1639)
                     /locus_tag="ACG827_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0482879.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACG827_000718"
     CDS             complement(1654..3420)
                     /locus_tag="ACG827_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0482880.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACG827_000719"
     CDS             3684..4361
                     /locus_tag="ACG827_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0482881.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACG827_000720"
     CDS             4364..5491
                     /locus_tag="ACG827_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0482882.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACG827_000721"
     CDS             complement(5691..6131)
                     /locus_tag="ACG827_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0482883.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACG827_000722"
     CDS             complement(6142..8175)
                     /locus_tag="ACG827_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0482884.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACG827_000723"
     CDS             complement(8177..9880)
                     /locus_tag="ACG827_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0482885.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG827_000724"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACG827_000724"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0482886.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACG827_000725"
     CDS             complement(10963..11988)
                     /locus_tag="ACG827_000725"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0482887.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACG827_000726"
     CDS             complement(12197..13066)
                     /locus_tag="ACG827_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0482888.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACG827_000727"
     CDS             complement(13525..15417)
                     /locus_tag="ACG827_000727"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482889.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACG827_000728"
     CDS             complement(15414..17141)
                     /locus_tag="ACG827_000728"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0482890.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACG827_000729"
     CDS             complement(17157..17807)
                     /locus_tag="ACG827_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0482891.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACG827_000730"
     CDS             18011..18307
                     /locus_tag="ACG827_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0482892.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACG827_000731"
     CDS             18479..19099
                     /locus_tag="ACG827_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0482893.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACG827_000732"
     CDS             complement(19191..20357)
                     /locus_tag="ACG827_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0482894.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACG827_000733"
     CDS             complement(20369..20722)
                     /locus_tag="ACG827_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0482895.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACG827_000734"
     CDS             20850..21872
                     /locus_tag="ACG827_000734"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0482896.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACG827_000735"
     CDS             21891..22052
                     /locus_tag="ACG827_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482897.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACG827_000736"
     CDS             complement(22311..22745)
                     /locus_tag="ACG827_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482898.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACG827_000737"
     CDS             complement(22855..24714)
                     /locus_tag="ACG827_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0482899.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACG827_000738"
     CDS             24715..24855
                     /locus_tag="ACG827_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0482900.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACG827_000739"
     CDS             complement(24832..25992)
                     /locus_tag="ACG827_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0482901.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG827_000740"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACG827_000740"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0482902.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACG827_000741"
     CDS             complement(27739..28437)
                     /locus_tag="ACG827_000741"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0482903.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACG827_000742"
     CDS             complement(28394..30055)
                     /locus_tag="ACG827_000742"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0482904.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACG827_000743"
     CDS             30273..31673
                     /locus_tag="ACG827_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0482905.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACG827_000744"
     CDS             complement(31925..33004)
                     /locus_tag="ACG827_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0482906.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACG827_000745"
     CDS             complement(33090..34049)
                     /locus_tag="ACG827_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0482907.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACG827_000746"
     CDS             complement(34061..35572)
                     /locus_tag="ACG827_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0482908.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACG827_000747"
     CDS             complement(35594..36157)
                     /locus_tag="ACG827_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0482909.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACG827_000748"
     CDS             complement(36163..37026)
                     /locus_tag="ACG827_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0482910.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACG827_000749"
     CDS             complement(37040..37936)
                     /locus_tag="ACG827_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0482911.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACG827_000750"
     CDS             complement(38111..39400)
                     /locus_tag="ACG827_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0482912.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACG827_000751"
     CDS             complement(39372..40889)
                     /locus_tag="ACG827_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0482913.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11811 a         8587 c         7096 g        13490 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgcc ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgt
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 tttt
//
LOCUS       DAZKOU010000015        38020 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000015 DAZKOU010000000
VERSION     DAZKOU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.015"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..288
                     /locus_tag="ACG827_000752"
     CDS             <1..288
                     /locus_tag="ACG827_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482914.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="ACG827_000753"
     CDS             347..787
                     /locus_tag="ACG827_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482915.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="ACG827_000754"
     CDS             799..1467
                     /locus_tag="ACG827_000754"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0482916.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="ACG827_000755"
     CDS             1531..2154
                     /locus_tag="ACG827_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0482917.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="ACG827_000756"
     CDS             2210..2596
                     /locus_tag="ACG827_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0482918.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="ACG827_000757"
     CDS             2809..4200
                     /locus_tag="ACG827_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0482919.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4811..5179
                     /locus_tag="ACG827_000758"
     CDS             4811..5179
                     /locus_tag="ACG827_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0482920.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5359..6300
                     /locus_tag="ACG827_000759"
     CDS             5359..6300
                     /locus_tag="ACG827_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0482921.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6369..7100
                     /locus_tag="ACG827_000760"
     CDS             6369..7100
                     /locus_tag="ACG827_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0482922.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7244..9424
                     /locus_tag="ACG827_000761"
     CDS             7244..9424
                     /locus_tag="ACG827_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0482923.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9444..9926
                     /locus_tag="ACG827_000762"
     CDS             9444..9926
                     /locus_tag="ACG827_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0482924.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9942..10703
                     /locus_tag="ACG827_000763"
     CDS             9942..10703
                     /locus_tag="ACG827_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0482925.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10834..12168
                     /gene="murC"
                     /locus_tag="ACG827_000764"
     CDS             10834..12168
                     /gene="murC"
                     /locus_tag="ACG827_000764"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0482926.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12475..12960
                     /locus_tag="ACG827_000765"
     CDS             12475..12960
                     /locus_tag="ACG827_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0482927.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12953..13636
                     /locus_tag="ACG827_000766"
     CDS             12953..13636
                     /locus_tag="ACG827_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0482928.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13762..16407
                     /gene="polA"
                     /locus_tag="ACG827_000767"
     CDS             13762..16407
                     /gene="polA"
                     /locus_tag="ACG827_000767"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0482929.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16452..17288
                     /gene="mutM"
                     /locus_tag="ACG827_000768"
     CDS             16452..17288
                     /gene="mutM"
                     /locus_tag="ACG827_000768"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0482930.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17252..17881
                     /gene="coaE"
                     /locus_tag="ACG827_000769"
     CDS             17252..17881
                     /gene="coaE"
                     /locus_tag="ACG827_000769"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0482931.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17933..18265
                     /locus_tag="ACG827_000770"
     CDS             17933..18265
                     /locus_tag="ACG827_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0482932.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18577..19068
                     /gene="nrdR"
                     /locus_tag="ACG827_000771"
     CDS             18577..19068
                     /gene="nrdR"
                     /locus_tag="ACG827_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0482933.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19091..20464
                     /locus_tag="ACG827_000772"
     CDS             19091..20464
                     /locus_tag="ACG827_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0482934.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20464..21390
                     /gene="dnaI"
                     /locus_tag="ACG827_000773"
     CDS             20464..21390
                     /gene="dnaI"
                     /locus_tag="ACG827_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0482935.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21519..21959
                     /locus_tag="ACG827_000774"
     CDS             21519..21959
                     /locus_tag="ACG827_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0482936.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22103..22804
                     /locus_tag="ACG827_000775"
     CDS             22103..22804
                     /locus_tag="ACG827_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0482937.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            22801..23922
                     /locus_tag="ACG827_000776"
     CDS             22801..23922
                     /locus_tag="ACG827_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0482938.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            23937..25169
                     /gene="pepT"
                     /locus_tag="ACG827_000777"
     CDS             23937..25169
                     /gene="pepT"
                     /locus_tag="ACG827_000777"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0482939.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25301..26209
                     /locus_tag="ACG827_000778"
     CDS             25301..26209
                     /locus_tag="ACG827_000778"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0482940.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26244..26516
                     /locus_tag="ACG827_000779"
     CDS             26244..26516
                     /locus_tag="ACG827_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0482941.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26527..27573
                     /locus_tag="ACG827_000780"
     CDS             26527..27573
                     /locus_tag="ACG827_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0482942.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            27824..30433
                     /gene="clpB"
                     /locus_tag="ACG827_000781"
     CDS             27824..30433
                     /gene="clpB"
                     /locus_tag="ACG827_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0482943.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30584..31270)
                     /locus_tag="ACG827_000782"
     CDS             complement(30584..31270)
                     /locus_tag="ACG827_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482944.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31248..31742)
                     /locus_tag="ACG827_000783"
     CDS             complement(31248..31742)
                     /locus_tag="ACG827_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0482945.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(31762..32982)
                     /locus_tag="ACG827_000784"
     CDS             complement(31762..32982)
                     /locus_tag="ACG827_000784"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0482946.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33129..34964
                     /gene="lepA"
                     /locus_tag="ACG827_000785"
     CDS             33129..34964
                     /gene="lepA"
                     /locus_tag="ACG827_000785"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0482947.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35160..36071
                     /locus_tag="ACG827_000786"
     CDS             35160..36071
                     /locus_tag="ACG827_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0482948.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(37011..37970)
                     /locus_tag="ACG827_000787"
     CDS             complement(37011..37970)
                     /locus_tag="ACG827_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0482949.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
BASE COUNT        12758 a         6592 c         8103 g        10567 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4381 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4441 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4501 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4561 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4621 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     4681 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     4741 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gatcgtttag
     4801 gaggaaacag atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa
     4861 aaatgcaacg ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg
     4921 gacgcttata attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg
     4981 agcatttgtt gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta
     5041 cagtaaagat atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga
     5101 tatggtacag cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt
     5161 tgcggttaca ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc
     5221 gaagtctttt taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta
     5281 tacacaagca aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa
     5341 gaaggaagga agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc
     5401 gaagaaccag aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa
     5461 ggtcgtattc tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac
     5521 acgactgcct atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag
     5581 atcgatccaa gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt
     5641 gtaagtttaa agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca
     5701 ccgcaagaat ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat
     5761 gattatgaat atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt
     5821 cgagagcttc cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg
     5881 acctactgta ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga
     5941 ttcaaagatg tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc
     6001 caaggagaat tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc
     6061 aaccaagtag ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga
     6121 tatatcaatt gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa
     6181 gaaaaacatt taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt
     6241 caagaccatg gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag
     6301 aaaacaatag ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg
     6361 tgatccacat gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt
     6421 ttgaaaaact tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc
     6481 tcaatgatcg aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag
     6541 aaacaggagc catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat
     6601 caccggagga ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc
     6661 tgattcaccc aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat
     6721 cgagatattc agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa
     6781 attatctttt tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc
     6841 taagagactt tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga
     6901 caattgtatt tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc
     6961 tctggattca ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc
     7021 atggggttgt ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag
     7081 gaccttggca tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct
     7141 ctttttattt ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat
     7201 gatagaataa agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa
     7261 ccagacgatc attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac
     7321 tactgtactt aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa
     7381 agaaatgact ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta
     7441 tcttgaaaac ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa
     7501 actgactccc gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga
     7561 tttgtatcgt ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg
     7621 acttgaacca ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc
     7681 agaaaaatat attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc
     7741 tcatgagatt attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc
     7801 ttatacgtat aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa
     7861 aggcgtatat gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg
     7921 aatattagct actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga
     7981 tgaagcagca attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc
     8041 ttcttcattc gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat
     8101 cgagcgagaa ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt
     8161 cggcgaaaac ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg
     8221 atttgatccg gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt
     8281 tttagcaatc gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc
     8341 tggaccagct tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa
     8401 tggtacagcc agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca
     8461 taaaacctat tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat
     8521 tgcacgaaaa gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg
     8581 tcgcctgcaa gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg
     8641 ccagtaccaa catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga
     8701 aacagcggtt aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca
     8761 tatatcagga ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg
     8821 agaattcacc gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga
     8881 acaagcaatc ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat
     8941 ccatcccgaa agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa
     9001 agagcttggt acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc
     9061 tgaaagccta tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc
     9121 tggacgagat atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat
     9181 ggaagacttg aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg
     9241 cgcatttgtt gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa
     9301 aaaattcgtc aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat
     9361 agaacaagtc gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga
     9421 gtaacaatga ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga
     9481 tatcattaaa aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac
     9541 gtacaactgg cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat
     9601 ttgaagagtc agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc
     9661 atcttcatct agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga
     9721 aaaaaacagg agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt
     9781 attatcgatg ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca
     9841 aatatcgttg tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact
     9901 ttaatgaagc acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga
     9961 ggaatctaaa agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt
    10021 ttgaggaact gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg
    10081 ataaagcagc acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg
    10141 tggggcatgc gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag
    10201 atggcatgag tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg
    10261 ataaggcttt tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac
    10321 tggagattct tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga
    10381 cactgcaacg caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt
    10441 tcgcgaatgc tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta
    10501 cagcaaaaga actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag
    10561 aaatggaacg tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc
    10621 ctcacattga tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa
    10681 aaacggattt ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt
    10741 tggttgaaat tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa
    10801 agaatggacg ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat
    10861 tttgttggta tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt
    10921 ctaaatgtcc aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa
    10981 gcaaatatca cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt
    11041 gcaggaaatg catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg
    11101 gaagtgattc gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct
    11161 gtaacaggct ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt
    11221 gtccgtccaa caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa
    11281 ttcttttcat ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat
    11341 gcaatcatga ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta
    11401 tttacagctt tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac
    11461 gatgcttatt tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa
    11521 aacgatgata tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc
    11581 tatcatgggg atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc
    11641 ttaaatgctt taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt
    11701 gcagaagaaa tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca
    11761 gacatgaccg tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat
    11821 ggcgcacgcc aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt
    11881 acgcgtacga ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt
    11941 tacttatgcg atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac
    12001 cttggagcga aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg
    12061 ttagattatc atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa
    12121 caagcgtatg aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc
    12181 agtgtcctta taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt
    12241 tgcaacttat gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat
    12301 caaaaacatg agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt
    12361 ttcacgacct ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta
    12421 cgctactttt ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac
    12481 ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta
    12541 tccttaacag aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat
    12601 gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc
    12661 gttccatcag tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga
    12721 ccaggatcac atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa
    12781 acactgactt ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt
    12841 tctgtagaag cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa
    12901 ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa
    12961 tccaagtaca tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg
    13021 tatgggactt gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca
    13081 attagcaact tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact
    13141 gattttagta atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg
    13201 atttattgcc tactctcttt tgaatggcat aacattagcg gttactcttg catactacga
    13261 tattggaaca gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt
    13321 tgtcggagta acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt
    13381 aatcggagtg atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga
    13441 gctgttcatc tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca
    13501 aagcatccgt aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt
    13561 catggcattg aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt
    13621 tggacggaac aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg
    13681 tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact
    13741 ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt
    13801 gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac
    13861 ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa
    13921 gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa
    13981 atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg
    14041 ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat
    14101 gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc
    14161 gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat
    14221 atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa
    14281 tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct
    14341 gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa
    14401 tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa
    14461 gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat
    14521 acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat
    14581 aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc
    14641 gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag
    14701 gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca
    14761 gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta
    14821 tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa
    14881 gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga
    14941 atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat
    15001 atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac
    15061 ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg
    15121 cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa
    15181 aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg
    15241 gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct
    15301 gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg
    15361 aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa
    15421 aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca
    15481 ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc
    15541 gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca
    15601 ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga
    15661 ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt
    15721 tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa
    15781 catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta
    15841 tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg
    15901 aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc
    15961 cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa
    16021 tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac
    16081 cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa
    16141 cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg
    16201 atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt
    16261 catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg
    16321 aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg
    16381 ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt
    16441 gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt
    16501 tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac
    16561 cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta
    16621 gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg
    16681 aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt
    16741 tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga
    16801 caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc
    16861 ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag
    16921 caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag
    16981 atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca
    17041 aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg
    17101 gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag
    17161 cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga
    17221 aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg
    17281 caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc
    17341 caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac
    17401 tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt
    17461 gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga
    17521 aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca
    17581 aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga
    17641 aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca
    17701 gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag
    17761 tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg
    17821 aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg
    17881 agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc
    17941 attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct
    18001 caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga
    18061 aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa
    18121 gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct
    18181 cccagaaagt gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta
    18241 tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat
    18301 gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta
    18361 agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg
    18421 ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg
    18481 acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat
    18541 cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat
    18601 aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt
    18661 cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga agctgctccg
    18721 ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga
    18781 ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc
    18841 aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt
    18901 ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca
    18961 agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg
    19021 gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg
    19081 aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag
    19141 aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact
    19201 aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc
    19261 tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc
    19321 gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa
    19381 agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa
    19441 agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg
    19501 attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa
    19561 aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca
    19621 agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga
    19681 agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc
    19741 aagtcttgat gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga
    19801 tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc
    19861 tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa
    19921 agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa
    19981 cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc
    20041 aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct
    20101 tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt
    20161 gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc
    20221 acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca
    20281 agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg
    20341 acgggaaaag cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga
    20401 gaaaaaagca gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat
    20461 ctagtggaag acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa
    20521 aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc
    20581 gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa
    20641 ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat
    20701 gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca
    20761 ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca
    20821 aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg
    20881 agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa
    20941 ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg
    21001 agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt
    21061 gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt
    21121 tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac
    21181 gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca
    21241 tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa
    21301 gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa
    21361 atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc
    21421 tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc
    21481 gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta
    21541 tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag
    21601 gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga
    21661 gacaaagcat atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa
    21721 cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt
    21781 cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa
    21841 atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga
    21901 tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac
    21961 caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt
    22021 taaggagata tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata
    22081 attatctaga aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt
    22141 gggtgacttt gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt
    22201 acccgttgca ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca
    22261 aggtccttat gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac
    22321 cgtacgcctt gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac
    22381 gattgcgggt atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca
    22441 acgcttaagc ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg
    22501 atcttggctg caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa
    22561 aaaaatatat gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat
    22621 ggagctgatg ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg
    22681 gacaagagag cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca
    22741 agaggcacgt caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc
    22801 atgacaatca gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta
    22861 gctgaagaag gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga
    22921 gtaatgatga cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc
    22981 gatttgctga ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat
    23041 cagccacaag aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca
    23101 cacacgaaca tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt
    23161 attgaagtgg agagttattt agtaaagact catgagattc attataaaaa actggccgtt
    23221 tatgtacctg tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt
    23281 acacaaggtg attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg
    23341 gaagctggtg cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca
    23401 aaagttgagg ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca
    23461 gctcatcctt atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg
    23521 tttgggttag gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa
    23581 caagtaaaag aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa
    23641 tcctctgtga agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca
    23701 atcgcccaaa gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat
    23761 atgcaaagcg ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag
    23821 caacgattta tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt
    23881 ttcgtttctg aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt
    23941 acgaaaatct tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa
    24001 caagcaaaac gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag
    24061 aattagaaga gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg
    24121 gaacattacc aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg
    24181 atacagcaga ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg
    24241 agtctacgat cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa
    24301 atttgaaaaa ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag
    24361 cagatgacaa atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc
    24421 cttctatccc tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg
    24481 gagcggataa attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg
    24541 gtcctttagg tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc
    24601 aagggaaaaa tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag
    24661 caatcgattt tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg
    24721 aaggtttctt ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata
    24781 tcattcgcga tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc
    24841 aagaagagtt gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat
    24901 attacaacat gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag
    24961 caatgatcga gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt
    25021 caaaaatctc ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc
    25081 acggacgatt cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa
    25141 aaattgcaga attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt
    25201 tcgtcccaat gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt
    25261 gtcccgccct cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt
    25321 ttataacaaa agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta
    25381 tgtaaaagaa aagcagccag aattatatgt agaactagct gaaactggcc catcgattga
    25441 tgatgcggaa ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg
    25501 tggagacggc acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca
    25561 tgtcggatta cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct
    25621 caaaaaagaa gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg
    25681 tcaagtcaat gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc
    25741 agtttggatc agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt
    25801 ttttaagtta ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca
    25861 ttggaaagga aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac
    25921 aaattttgac gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc
    25981 cttagatatt ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac
    26041 gatccccggc attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc
    26101 tgtgggcact cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt
    26161 atcaaaagga ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt
    26221 aacagaaagt tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc
    26281 ttttcattgt gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa
    26341 ctagcggctg gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt
    26401 tgggaggatt gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta
    26461 taggattcgc tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag
    26521 caggagatgt cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt
    26581 gtgatttttg ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa
    26641 ttgctgtcta ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa
    26701 atcgctgaag ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg
    26761 aaagatgggt tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca
    26821 ggaacgctga tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga
    26881 gcaggggtga cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta
    26941 gctggcttgg tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt
    27001 atcatcgctg gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc
    27061 agcttgaatg ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt
    27121 ttgcggaaat gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa
    27181 accattatcc aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt
    27241 gtattttggt atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt
    27301 ctactgacca tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct
    27361 gctggtattg gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca
    27421 gttgacgcag tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc
    27481 tatggatttg tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa
    27541 gtaaaaaaag agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat
    27601 caaatcaaag aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat
    27661 atacatacaa tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa
    27721 actatgtgta tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca
    27781 aagaaataag tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat
    27841 gactacgacg cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca
    27901 tcaagatatc gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg
    27961 aaatttctat acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct
    28021 actggatgag tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca
    28081 gaatttcttt cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt
    28141 tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt
    28201 ttatttgaat aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag
    28261 aggaggagaa agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata
    28321 cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga
    28381 tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct
    28441 gataggagaa cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt
    28501 acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc
    28561 attgatcgct ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa
    28621 agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt
    28681 aggggcagga aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc
    28741 tcgcggtgaa ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga
    28801 aaaagacaaa gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga
    28861 agatacgatc agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa
    28921 catccatgac aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg
    28981 gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga
    29041 aatgaactct atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat
    29101 cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt
    29161 gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac
    29221 agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca
    29281 cgaattagaa gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg
    29341 tacgatacct caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga
    29401 gattaagatg gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt
    29461 aacgggtatc cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa
    29521 tgaaacactt cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc
    29581 ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt
    29641 cctaggacca actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt
    29701 tgactcagaa gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt
    29761 gtctcgtcta gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac
    29821 ggaagctgtc agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca
    29881 tcccgatgtc ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa
    29941 agggcgagta gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca
    30001 gttactgctg gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt
    30061 caacacgcta ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat
    30121 tttatttaca ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca
    30181 attggcacaa cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac
    30241 gtggattgct gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat
    30301 cactaaggaa gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa
    30361 atcaaaagta acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga
    30421 agaaatcgtc taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt
    30481 gtcagacctc cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc
    30541 cgaaatttct tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt
    30601 tttctaattt ataactttca aagtaacgct catctttttt actttgagac catgcaaaca
    30661 atcccattaa ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg
    30721 ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta
    30781 agaactggtc gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg
    30841 caaagccgaa taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca
    30901 agtaaccaat caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga
    30961 ttttgtccca aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga
    31021 tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt
    31081 tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg
    31141 tgattccttg agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa
    31201 cgaatacacc aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa
    31261 gtccctccat ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact
    31321 tttccgttca atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca
    31381 gctgtttctt gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca
    31441 gtatataagc cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc
    31501 ccggcatcga tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt
    31561 tctgtgtcag caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct
    31621 tcattttctt gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact
    31681 aactgttggt tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc
    31741 atctaaacat catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat
    31801 gatacgctct agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa
    31861 ataagaagca cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac
    31921 ggattttcct ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc
    31981 gaagacacca ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc
    32041 tgttccaaca aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa
    32101 atatggttcg atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt
    32161 tttataaata tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca
    32221 taagccattt cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc
    32281 agctacccag tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc
    32341 tgtgcgctca tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa
    32401 taaagcacca tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc
    32461 aactggtaat cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct
    32521 gattttaaag gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat
    32581 actgtaagga tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac
    32641 gataattaga tcttgcatca atccatggtt agcatattct ggataagttc caactccagt
    32701 cacaccaccc attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat
    32761 ttgagagatc agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg
    32821 ttttttggaa cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc
    32881 agtcacttgg aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac
    32941 cggttttaat tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat
    33001 tagcttgata acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa
    33061 aactctttaa attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag
    33121 gagacgctat ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga
    33181 tcatcgctca tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc
    33241 acactgtgac gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg
    33301 aacgtggaat cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag
    33361 aaacatatat ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct
    33421 ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg
    33481 aagcacaaac attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag
    33541 tcatcaataa aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag
    33601 acgtaatcgg aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga
    33661 ttgaagatat tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg
    33721 caccgctaaa agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa
    33781 atgtccggat cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg
    33841 gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact
    33901 ttttgatggt cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac
    33961 gagtagggga tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc
    34021 gaaaaatgaa tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg
    34081 atttgcggga agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag
    34141 aaacctctca agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg
    34201 atgtcgtcca agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat
    34261 ccgttattta ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag
    34321 attttcctga acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca
    34381 tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat
    34441 tcattacgat ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt
    34501 ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat
    34561 tggattatga aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga
    34621 atggagaaaa agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg
    34681 gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa
    34741 tccaagcagc tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa
    34801 acgtcttggc taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac
    34861 aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct
    34921 tcatggccgt tctgaaaatg gatgagcaag atagtgtgaa ataacttata atcaatcgaa
    34981 aaacctatta aataaacctt tttgagtaga tgcaacactg aggattatct gtataaaaga
    35041 ttttaaactg tcatttggtt atcacggcga tactaaaatt aacaaaattg gaatattttt
    35101 cttgaagatt tttagctgtc agtatatgat gaaagcatct cataggaggt gcttttttca
    35161 tggttgataa ttcaagtgga agggttctta aatctctaag aaaagagaaa aaactatctc
    35221 aaaaaaagtt agctgattta gcaggtattt cacaaagtac tttagttaaa tatgaaaaag
    35281 ggtcaagaaa aatccctaaa gacgtagata atactctaag caagattctg aatatagaaa
    35341 ctctaataaa agatgaggaa gataagattg agattctcat cggtaaacta attgcataca
    35401 gagatatgaa taagttactt aacaaagagt tagctgataa tataggaatt agtgaagtat
    35461 tactcagtta tgtgttaaat agaaagagaa atccgtcaaa agaaatgcaa aaaaaaatcg
    35521 atatttttct attaagtaat gaaaaagaaa ttttgaagga gattaatcgt gatagtgaga
    35581 tatttagttt atcaaaagac gataaaattg ttatgggaaa aagaatacga gaagtccgaa
    35641 agaaccggga agagacgctt gaaaaatttg gtaaaaactt taccatctat acaggcaaaa
    35701 atgttataag tagatgggaa aaaggtatta atattccaga tattgaaaaa ctaatgaata
    35761 ttgcctatct tggtaaggtc actgttcctt accttatgta cggagaagat tataagaata
    35821 tacttccaaa agatgaaaga gttagcgatt ttaaaaagat aaattctttt agtatgggat
    35881 taaggatgag gaaaattagg aaagattatt atctagaaag ggaggagttt ggaaagcttt
    35941 tttctccttc aatttcaaaa tggtccatag ataggtatga gaatggtaga gatataccca
    36001 atactaatcg aattatccaa tatgcttata taggaaatct ttctttagaa tttttgattt
    36061 acgggatata aaaatagttt acgtcgatac ttattaagtt atcgacgttt tttattatag
    36121 aaaaaattta taaaaggagg tttcttgata tgataagaaa caaaaagggt ttgttgaaag
    36181 ttgctttact ggcagctttt ttctaagtaa ctttttgaca agctcaatgg caacacaagc
    36241 ggaagaagta atacaagaac taactgttat gaataacagc tagataaaat gggctatgta
    36301 tgttatccaa caatttagta atcactagaa gcctagcgaa agttcgcttg ataagttata
    36361 gatgagtatt gatttgtagt gtttccaaat caaaagggta cagagccaca tgaaatatgt
    36421 tgtcctctgc agcgtcagga tgggaatcca taccatggag attcggagat ctgtgaggga
    36481 atacttacgt ggttaatatg tcagttgtgt tggacgtagt aatggtcata gtgggtagat
    36541 tgactgaatt cttatatgag aaaatgaaac caacaagaag atagggttat ttatacagaa
    36601 ttggggatct tttctggaaa agtaatcgga taatgcgaga ttcgtacggt cttttttaaa
    36661 tttctctaaa aaaaacggca tcgaatgggt tgggtgattg ataagcaatg aatcatagac
    36721 tgatatagaa taaaaaatga ttctatttat ctttttacat ctatcgtttt ttcaagtagc
    36781 cgcaaattat atcagcattc aacaattttt gtatacaagt tctagtaaaa ggatctgaag
    36841 aaaaagttgg ttgttgtgaa gataaaaaaa gaatcaggtt ttactgagtt tttcttcaaa
    36901 atttgtgtta ttaggttagg acaaagataa gaagaagtat gaagatttaa aaaaggaatg
    36961 aggaaaagat agtaatttcc gattattgga gtttttttat ttttcgtttg tcaaattaag
    37021 ttagacaaat catcttttgt gacgtaactc aaaaatactt ccattggtgt ccgatagtgt
    37081 aatgattttc taggaatatg atttcgtttc gatgccaccg attggataaa ggtttcagat
    37141 acaccattaa aatccatagc tttatacaaa ccatctttac gtaataagcc atttgaatgt
    37201 tcattcaatc ctcgttgaga aggtgttcct ggatccgcaa aataaattga aatatcgttg
    37261 gtattactaa tctttttcca atttgagaat tcttttccac aatcaaatgt gatggattta
    37321 aataagtttt ttgggacaca acgaaaccat tgattcagcc tattttcaat gtctttagcc
    37381 tgtctgcctt cgggtttcaa ggtaatgatg actttagata gccgttcaac taatgtgatg
    37441 accgcgcttt tatgttttcc acccacgata gtgtcacctt ccaggtgacc aaattcatga
    37501 tcaaactggg tatagtctgt ctttctgtct cggattgacc ggcgaaaccc ttgttttcct
    37561 cttttttctt tatgaccgtt tggttttctt tttcccttca ttggtaaatc gtgagtgtca
    37621 aaggttttat ctttaaacat tcgatacagt gtgcgtaccg aacaagcaat aggaaactct
    37681 gctcgaccaa taaggacatc aggagcccac ccttgaacaa cctttttttg aatgtattcc
    37741 tgttgctcat caggtaaaac aatcggatgt cttccgcagt tacgtttgtt tttcttatat
    37801 tgtttatagt agctcattgc gcttccacca gatttaagaa aacgatagac cttataaata
    37861 gtttgtcgag aacgttgtaa cagattggct acttttgaaa caggttgtga ttgattgaaa
    37921 taagcttcta tcattacgag ttcagttggt gtaagatggt tataggtcat ttgtactcac
    37981 tccttataat tctttcgtcg gaactatttt gagtgtagca
//
LOCUS       DAZKOU010000016        37151 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000016 DAZKOU010000000
VERSION     DAZKOU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.016"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..2301)
                     /locus_tag="ACG827_000788"
     CDS             complement(148..2301)
                     /locus_tag="ACG827_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0482950.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2595..3443)
                     /locus_tag="ACG827_000789"
     CDS             complement(2595..3443)
                     /locus_tag="ACG827_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0482951.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="ACG827_000790"
     CDS             complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="ACG827_000790"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0482952.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5944..6123)
                     /locus_tag="ACG827_000791"
     CDS             complement(5944..6123)
                     /locus_tag="ACG827_000791"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0482953.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6170..7993)
                     /locus_tag="ACG827_000792"
     CDS             complement(6170..7993)
                     /locus_tag="ACG827_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0482954.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8271..10550
                     /gene="helD"
                     /locus_tag="ACG827_000793"
     CDS             8271..10550
                     /gene="helD"
                     /locus_tag="ACG827_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0482955.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10643..11290)
                     /locus_tag="ACG827_000794"
     CDS             complement(10643..11290)
                     /locus_tag="ACG827_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482956.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11469..12188)
                     /locus_tag="ACG827_000795"
     CDS             complement(11469..12188)
                     /locus_tag="ACG827_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0482957.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12319..13470)
                     /locus_tag="ACG827_000796"
     CDS             complement(12319..13470)
                     /locus_tag="ACG827_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0482958.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13687..14427)
                     /locus_tag="ACG827_000797"
     CDS             complement(13687..14427)
                     /locus_tag="ACG827_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0482959.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="ACG827_000798"
     CDS             complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="ACG827_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0482960.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14811..15404)
                     /gene="yqeK"
                     /locus_tag="ACG827_000799"
     CDS             complement(14811..15404)
                     /gene="yqeK"
                     /locus_tag="ACG827_000799"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0482961.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15388..16038)
                     /locus_tag="ACG827_000800"
     CDS             complement(15388..16038)
                     /locus_tag="ACG827_000800"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0482962.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="ACG827_000801"
     CDS             complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="ACG827_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0482963.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="ACG827_000802"
     CDS             complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="ACG827_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0482964.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17481..18008)
                     /locus_tag="ACG827_000803"
     CDS             complement(17481..18008)
                     /locus_tag="ACG827_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0482965.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18188..18973)
                     /locus_tag="ACG827_000804"
     CDS             complement(18188..18973)
                     /locus_tag="ACG827_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0482966.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18966..19835)
                     /gene="accD"
                     /locus_tag="ACG827_000805"
     CDS             complement(18966..19835)
                     /gene="accD"
                     /locus_tag="ACG827_000805"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0482967.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19836..21215)
                     /locus_tag="ACG827_000806"
     CDS             complement(19836..21215)
                     /locus_tag="ACG827_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0482968.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="ACG827_000807"
     CDS             complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="ACG827_000807"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0482969.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21667..22143)
                     /gene="accB"
                     /locus_tag="ACG827_000808"
     CDS             complement(21667..22143)
                     /gene="accB"
                     /locus_tag="ACG827_000808"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0482970.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="ACG827_000809"
     CDS             complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="ACG827_000809"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0482971.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="ACG827_000810"
     CDS             complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="ACG827_000810"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0482972.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="ACG827_000811"
     CDS             complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="ACG827_000811"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0482973.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25173..25397)
                     /locus_tag="ACG827_000812"
     CDS             complement(25173..25397)
                     /locus_tag="ACG827_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0482974.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25450..26412)
                     /locus_tag="ACG827_000813"
     CDS             complement(25450..26412)
                     /locus_tag="ACG827_000813"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0482975.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26422..26862)
                     /locus_tag="ACG827_000814"
     CDS             complement(26422..26862)
                     /locus_tag="ACG827_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0482976.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27168..28133
                     /locus_tag="ACG827_000815"
     CDS             27168..28133
                     /locus_tag="ACG827_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0482977.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28190..28420)
                     /locus_tag="ACG827_000816"
     CDS             complement(28190..28420)
                     /locus_tag="ACG827_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0482978.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28506..29393)
                     /locus_tag="ACG827_000817"
     CDS             complement(28506..29393)
                     /locus_tag="ACG827_000817"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0482979.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29482..30705)
                     /locus_tag="ACG827_000818"
     CDS             complement(29482..30705)
                     /locus_tag="ACG827_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0482980.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30722..31768)
                     /locus_tag="ACG827_000819"
     CDS             complement(30722..31768)
                     /locus_tag="ACG827_000819"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0482981.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="ACG827_000820"
     CDS             complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="ACG827_000820"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0482982.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(33659..33925)
                     /locus_tag="ACG827_000821"
     CDS             complement(33659..33925)
                     /locus_tag="ACG827_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0482983.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34404..36638
                     /locus_tag="ACG827_000822"
     CDS             34404..36638
                     /locus_tag="ACG827_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0482984.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36786..37106
                     /locus_tag="ACG827_000823"
     CDS             36786..37106
                     /locus_tag="ACG827_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0482985.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        10450 a         8038 c         6479 g        12184 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
     2521 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
     2581 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
     2641 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
     2701 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
     2761 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
     2821 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
     2881 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
     2941 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
     3001 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
     3061 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
     3121 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
     3181 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
     3241 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
     3301 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     3361 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     3421 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     3481 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     3541 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     3601 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     3661 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     3721 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     3781 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     3841 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     3901 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     3961 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     4021 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     4081 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     4141 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     4201 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     4261 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     4321 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     4381 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     4441 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     4501 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     4561 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     4621 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     4681 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     4741 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     4801 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     4861 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     4921 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     4981 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     5041 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     5101 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     5161 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     5221 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     5281 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     5341 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     5401 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     5461 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     5521 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     5581 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     5641 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     5701 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     5761 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     5821 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     5881 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     5941 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     6001 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     6061 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     6121 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     6181 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     6241 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     6301 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     6361 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     6421 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     6481 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     6541 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     6601 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     6661 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     6721 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     6781 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     6841 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     6901 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     6961 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     7021 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     7081 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     7141 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     7201 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     7261 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     7321 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     7381 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     7441 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     7501 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     7561 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     7621 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     7681 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     7741 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     7801 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     7861 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     7921 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     7981 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     8041 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     8101 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     8161 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     8221 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     8281 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     8341 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     8401 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     8461 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     8521 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     8581 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     8641 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     8701 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     8761 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     8821 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     8881 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     8941 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     9001 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     9061 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     9121 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     9181 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     9241 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     9301 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     9361 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     9421 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     9481 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     9541 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     9601 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     9661 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     9721 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     9781 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     9841 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     9901 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     9961 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
    10021 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
    10081 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
    10141 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
    10201 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
    10261 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
    10321 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
    10381 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
    10441 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
    10501 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
    10561 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
    10621 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
    10681 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
    10741 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
    10801 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
    10861 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
    10921 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
    10981 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
    11041 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
    11101 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
    11161 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
    11221 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
    11281 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
    11341 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
    11401 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
    11461 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
    11521 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
    11581 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
    11641 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
    11701 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
    11761 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
    11821 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
    11881 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
    11941 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
    12001 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
    12061 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
    12121 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
    12181 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
    12241 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
    12301 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
    12361 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
    12421 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
    12481 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
    12541 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
    12601 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    12661 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    12721 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
    12781 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    12841 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    12901 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    12961 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    13021 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    13081 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    13141 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    13201 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    13261 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    13321 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
    13381 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    13441 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
    13501 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    13561 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    13621 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    13681 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    13741 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    13801 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    13861 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    13921 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    13981 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    14041 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    14101 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    14161 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    14221 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    14281 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    14341 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
    14401 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    14461 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
    14521 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    14581 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    14641 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    14701 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
    14761 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    14821 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    14881 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    14941 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    15001 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    15061 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    15121 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
    15181 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    15241 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    15301 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    15361 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    15421 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    15481 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    15541 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    15601 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    15661 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    15721 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
    15781 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    15841 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    15901 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    15961 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    16021 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    16081 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    16141 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    16201 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    16261 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    16321 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    16381 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    16441 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    16501 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    16561 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    16621 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    16681 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    16741 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    16801 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    16861 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    16921 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    16981 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    17041 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    17101 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    17161 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    17221 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
    17281 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct cattataatg
    17341 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    17401 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    17461 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    17521 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    17581 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    17641 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    17701 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    17761 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    17821 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    17881 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    17941 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    18001 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    18061 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    18121 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    18181 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
    18241 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
    18301 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
    18361 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
    18421 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
    18481 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
    18541 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
    18601 cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca
    18661 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
    18721 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
    18781 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
    18841 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
    18901 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
    18961 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
    19021 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
    19081 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
    19141 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
    19201 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
    19261 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
    19321 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
    19381 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
    19441 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
    19501 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
    19561 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
    19621 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
    19681 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
    19741 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
    19801 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
    19861 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
    19921 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
    19981 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
    20041 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
    20101 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
    20161 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
    20221 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
    20281 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
    20341 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
    20401 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
    20461 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
    20521 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
    20581 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
    20641 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    20701 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    20761 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    20821 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    20881 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    20941 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    21001 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    21061 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    21121 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    21181 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    21241 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    21301 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    21361 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    21421 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    21481 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    21541 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    21601 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    21661 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    21721 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    21781 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    21841 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    21901 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    21961 ttcaggtatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    22021 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    22081 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    22141 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    22201 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    22261 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    22321 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    22381 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    22441 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    22501 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    22561 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    22621 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    22681 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    22741 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    22801 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    22861 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    22921 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    22981 ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat aacttggttt
    23041 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    23101 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    23161 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    23221 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    23281 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    23341 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    23401 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    23461 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    23521 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    23581 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    23641 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    23701 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    23761 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    23821 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    23881 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    23941 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    24001 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    24061 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    24121 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    24181 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    24241 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    24301 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    24361 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    24421 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    24481 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    24541 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    24601 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    24661 tcggcgcatt catcactgca accattttcc cgcttccagc tggggcagct tctgtcatat
    24721 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    24781 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    24841 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    24901 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    24961 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    25021 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    25081 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    25141 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    25201 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    25261 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    25321 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    25381 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    25441 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    25501 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    25561 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    25621 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    25681 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    25741 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    25801 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    25861 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    25921 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    25981 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    26041 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    26101 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    26161 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    26221 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    26281 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    26341 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    26401 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    26461 tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat
    26521 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    26581 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    26641 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    26701 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    26761 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    26821 actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat
    26881 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    26941 ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca
    27001 actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct cacatatttt
    27061 gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt gtccattatt
    27121 tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa
    27181 aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga gatggtaagg
    27241 tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat tttcatcaga
    27301 ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat tttgatttcg
    27361 tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc caacattacc
    27421 gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta catattacca
    27481 ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct tttcgtctct
    27541 ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt
    27601 ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa atgacttttg
    27661 aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag ctactgactt
    27721 acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa gccggtatgc
    27781 ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa caaaagcagt
    27841 ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag agtcagaaaa
    27901 agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct ggtgacgttt
    27961 ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt caattgtcaa
    28021 aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta gaaaaagaac
    28081 agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc
    28141 tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt tattggatta
    28201 agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc tgatgagtat
    28261 ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta cacttctcta
    28321 cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg accatagcct
    28381 ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct ccttttctta
    28441 tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct aaaaaaactc
    28501 gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt gatcaaagct
    28561 tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt caatgcttgg
    28621 gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat ctttaaatct
    28681 ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa
    28741 taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat cactttcttt
    28801 aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt catcgtacct
    28861 gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga accatgcaat
    28921 gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac ggcttcttct
    28981 ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc ggataccggt
    29041 gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc tgccaggctt
    29101 ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga aaaaatcccc
    29161 gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt aaagagaata
    29221 ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc tgttactaaa
    29281 ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc ttctagtaga
    29341 tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt cattttttcg
    29401 acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa caaggatttt
    29461 ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt tgattcgttc
    29521 aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg
    29581 ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa gtttagcttc
    29641 taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg ctggtgcaaa
    29701 gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc ggttcaaata
    29761 agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat aagtaagccc
    29821 ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat aaagtgctac
    29881 atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca gttctgagat
    29941 caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc tggcgttagg
    30001 ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt tctcataaga
    30061 aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg actcccttaa
    30121 tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt cgataccagt
    30181 aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa taacacgttc
    30241 acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt attttacatg
    30301 ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat acatcgtatg
    30361 gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa gtatccctgc
    30421 tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa ccaaataagc
    30481 ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg gtacacttgg
    30541 cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg taaaaatata
    30601 gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc
    30661 gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa
    30721 actagtcctt gacctgattt acttttttta atttgctaga gactttttca ttcagtacac
    30781 tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct tttgctgttt
    30841 gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg atcagtcgga
    30901 taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac cagccattat
    30961 acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct aaaacaacga
    31021 tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa tacgcacctt
    31081 ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga tcttctttta
    31141 caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac caaataaatg
    31201 tgtattcggg catcttctta gcaatttcta cgaaatcaat gattccttta cgttcgaaaa
    31261 acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat tctctgaact
    31321 tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg gaaatagctt
    31381 gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt gttggcgtga
    31441 tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata ggagaaagct
    31501 ggttcgatcc gataaaggaa tttcggaaat cttcctctgt agaatgcgca tgatagacaa
    31561 ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt ccgtatgtat
    31621 tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata ccgacttccg
    31681 tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca agcatctttt
    31741 cactttcaaa atataataat accttcacct tacgtccccc ttcacttata tagtataaca
    31801 tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta ctgacaaaaa
    31861 cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca caccagcttt
    31921 tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc catcgtatcg
    31981 cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa gcgtttcatt
    32041 aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag acccatacct
    32101 actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc tgcccatttt
    32161 ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga tgggttatat
    32221 ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt gtattgaatc
    32281 aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc tgctaagact
    32341 gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac ttccttgcct
    32401 tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg gaattctttg
    32461 agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc acgaagcaat
    32521 gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat acgcaatgca
    32581 cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag ttctttatct
    32641 ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc taatacagct
    32701 ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat atacaggaat
    32761 tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac tagatctttt
    32821 ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac agtttctgca
    32881 ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat
    32941 tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc gttgacagcc
    33001 aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat tgctggaatt
    33061 tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc tgttacaaac
    33121 gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc atgtgcaaca
    33181 acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa taagtgcgct
    33241 aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata agcattatct
    33301 tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc tgattcagca
    33361 tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg atctgaaaga
    33421 accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg cgctgctttt
    33481 tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa acgagcttct
    33541 tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg aactagcagg
    33601 taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat ttcaaccatt
    33661 attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca tcagcttcgt
    33721 cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa gacataacgc
    33781 ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca tcagagttaa
    33841 atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca gtttctgcta
    33901 ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt gttgttttta
    33961 tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc ctttcatctg
    34021 taaagattaa catttttttt cgttagatac aacagttttc attcaatttc agataaaagt
    34081 attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac aaacgtgttt
    34141 atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc ctttgaccct
    34201 ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta taataagagt
    34261 caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat ctcttaaaaa
    34321 actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat gtacccattt
    34381 ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat gaggcaacga
    34441 ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt cagagctgtt
    34501 atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa aatgatccat
    34561 ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg caacagcaag
    34621 gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc aatggtggtc
    34681 agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat atcacagaac
    34741 aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc aaacgagtca
    34801 ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa ccaggtgttg
    34861 gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat gtaccgcaaa
    34921 aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa ggtactggta
    34981 tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt caagcagaaa
    35041 atgtgatcct attcatagat gaagttcacg aaatagtcgg tgctggttct gctggtgatg
    35101 gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa ttgcaaatgg
    35161 ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct ttagaacgtc
    35221 gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt attttgaaag
    35281 gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag gccatcgaag
    35341 cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat aaagccatcg
    35401 atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt gatcctaaaa
    35461 caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct cgagaagaag
    35521 atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa atgaaagaac
    35581 gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa aaaatcgttg
    35641 aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca caactgaaaa
    35701 acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta gatcgagtag
    35761 cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca atcggctcct
    35821 tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa cttgcctacg
    35881 aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat atggaaaaac
    35941 atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa gaagctggtc
    36001 aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat gaagtagaaa
    36061 aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt cgattgacag
    36121 atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca agtaatgccg
    36181 gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt gttactcgtt
    36241 ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt tttgacggga
    36301 tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca ctaatgctag
    36361 acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct acgaatgtca
    36421 aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct cttcgacgca
    36481 cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac ccttctatcc
    36541 atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct aaaccagaac
    36601 gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat agcgtgagac
    36661 ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa tactgttcat
    36721 tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata aaagaggtga
    36781 gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta aaccagcttg
    36841 aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact attgtctaca
    36901 ctgaagcccc tcaacataat gaaattctgc tgatcaatga caaacgtaaa atccagccga
    36961 aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc gtttaccacg
    37021 aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt ccaaaacatg
    37081 aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa agagcgtggg
    37141 acaaaagtaa a
//
LOCUS       DAZKOU010000017        37110 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000017 DAZKOU010000000
VERSION     DAZKOU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.017"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..384)
                     /locus_tag="ACG827_000824"
     CDS             complement(<1..384)
                     /locus_tag="ACG827_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0482986.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="ACG827_000825"
     CDS             complement(569..967)
                     /locus_tag="ACG827_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0482987.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="ACG827_000826"
     CDS             complement(964..1737)
                     /locus_tag="ACG827_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0482988.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="ACG827_000827"
     CDS             complement(1734..2417)
                     /locus_tag="ACG827_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0482989.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="ACG827_000828"
     CDS             complement(2584..3471)
                     /locus_tag="ACG827_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0482990.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="ACG827_000829"
     CDS             complement(3468..4337)
                     /locus_tag="ACG827_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0482991.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="ACG827_000830"
     CDS             complement(4474..5760)
                     /locus_tag="ACG827_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0482992.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6033..6611
                     /locus_tag="ACG827_000831"
     CDS             6033..6611
                     /locus_tag="ACG827_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0482993.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6611..7540
                     /locus_tag="ACG827_000832"
     CDS             6611..7540
                     /locus_tag="ACG827_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0482994.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7537..9273
                     /locus_tag="ACG827_000833"
     CDS             7537..9273
                     /locus_tag="ACG827_000833"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0482995.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ACG827_000834"
     CDS             complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ACG827_000834"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0482996.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10169..10678)
                     /locus_tag="ACG827_000835"
     CDS             complement(10169..10678)
                     /locus_tag="ACG827_000835"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0482997.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ACG827_000836"
     CDS             complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ACG827_000836"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0482998.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11979..13070)
                     /locus_tag="ACG827_000837"
     CDS             complement(11979..13070)
                     /locus_tag="ACG827_000837"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0482999.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ACG827_000838"
     CDS             complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ACG827_000838"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0483000.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ACG827_000839"
     CDS             complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ACG827_000839"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0483001.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ACG827_000840"
     CDS             complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ACG827_000840"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0483002.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17033..18007)
                     /locus_tag="ACG827_000841"
     CDS             complement(17033..18007)
                     /locus_tag="ACG827_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0483003.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17988..19871)
                     /locus_tag="ACG827_000842"
     CDS             complement(17988..19871)
                     /locus_tag="ACG827_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0483004.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19889..22114)
                     /locus_tag="ACG827_000843"
     CDS             complement(19889..22114)
                     /locus_tag="ACG827_000843"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0483005.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22229..23263
                     /locus_tag="ACG827_000844"
     CDS             22229..23263
                     /locus_tag="ACG827_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0483006.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23330..24343)
                     /locus_tag="ACG827_000845"
     CDS             complement(23330..24343)
                     /locus_tag="ACG827_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0483007.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24421..24609)
                     /locus_tag="ACG827_000846"
     CDS             complement(24421..24609)
                     /locus_tag="ACG827_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0483008.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(25049..25177)
                     /locus_tag="ACG827_000847"
     CDS             complement(25049..25177)
                     /locus_tag="ACG827_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0483009.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="ACG827_000848"
     CDS             complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="ACG827_000848"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0483010.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26664..27719)
                     /locus_tag="ACG827_000849"
     CDS             complement(26664..27719)
                     /locus_tag="ACG827_000849"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0483011.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27729..29159)
                     /gene="gatB"
                     /locus_tag="ACG827_000850"
     CDS             complement(27729..29159)
                     /gene="gatB"
                     /locus_tag="ACG827_000850"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0483012.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(29156..30628)
                     /gene="gatA"
                     /locus_tag="ACG827_000851"
     CDS             complement(29156..30628)
                     /gene="gatA"
                     /locus_tag="ACG827_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0483013.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30628..30933)
                     /gene="gatC"
                     /locus_tag="ACG827_000852"
     CDS             complement(30628..30933)
                     /gene="gatC"
                     /locus_tag="ACG827_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0483014.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(31039..33075)
                     /gene="ligA"
                     /locus_tag="ACG827_000853"
     CDS             complement(31039..33075)
                     /gene="ligA"
                     /locus_tag="ACG827_000853"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0483015.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(33092..35326)
                     /gene="pcrA"
                     /locus_tag="ACG827_000854"
     CDS             complement(33092..35326)
                     /gene="pcrA"
                     /locus_tag="ACG827_000854"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0483016.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35481..37040
                     /locus_tag="ACG827_000855"
     CDS             35481..37040
                     /locus_tag="ACG827_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0483017.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        10252 a         8058 c         6689 g        12111 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa
     5881 aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg
     5941 gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa
     6001 attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg
     6061 atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc
     6121 aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa
     6181 tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc
     6241 tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg
     6301 atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga
     6361 gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca
     6421 acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg
     6481 ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt
     6541 atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac
     6601 ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga
     6661 agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa
     6721 gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca
     6781 agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca
     6841 taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca
     6901 gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga
     6961 acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca
     7021 tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa
     7081 tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt
     7141 cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa
     7201 tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta
     7261 tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct
     7321 ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg
     7381 tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga
     7441 gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat
     7501 gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat
     7561 gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc
     7621 atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg
     7681 aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag
     7741 aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca
     7801 gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt
     7861 tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact
     7921 tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt
     7981 gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg
     8041 actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg
     8101 aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt
     8161 caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc
     8221 atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca
     8281 gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag
     8341 gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt
     8401 tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg
     8461 tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa acggctagag
     8521 gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca attgctaggg
     8581 aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac gatccaagac
     8641 gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca gccccatttt
     8701 atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac agatgaagca
     8761 gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa aaaagaagaa
     8821 gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct tttgagtatg
     8881 cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca gtcattatct
     8941 atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc ttttcacaat
     9001 aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa
     9061 gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa tgatcatttt
     9121 tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg cgatgcatat
     9181 ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg tctaccgctt
     9241 cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg atttttttat
     9301 tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc ttctactgtc
     9361 agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat ttcttggagc
     9421 gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa aaaatattct
     9481 cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc ccgccagcca
     9541 aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt caagatgaca
     9601 ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa
     9661 cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa tccaacatgc
     9721 tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc tgctacatac
     9781 attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata atgcgtttct
     9841 aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt
     9901 gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt aacttcgact
     9961 tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa tgaattctct
    10021 acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg
    10081 gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg ataagtgaat
    10141 gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct ataatggtat
    10201 cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca ctttcttcat
    10261 accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt ccaagagcta
    10321 aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc gcttctgcct
    10381 ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca ctttggacaa
    10441 tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa atatctgact
    10501 ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc gacaattgga
    10561 tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat tgagctaacc
    10621 ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg ctagccatga
    10681 gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata agagattgaa
    10741 acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc
    10801 atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag aggtgtcggc
    10861 ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt ctgtaattct
    10921 tctgccaccg catcaattct gttggtttct tttactttta attcttctgc gtctttgatg
    10981 atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc atcaatcaga
    11041 cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt gacacttaaa
    11101 tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc catctcctcc
    11161 aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa caacagttgg
    11221 ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat atcacctgga
    11281 acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt tttatcttcc
    11341 actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg ggaacgttct
    11401 ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa agcagatttg
    11461 acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg ggttcctgtc
    11521 acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc catctcgcgt
    11581 aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc gaacagagta
    11641 gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt accggagttt
    11701 cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc atgaatcttg
    11761 atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg taaagtactc
    11821 agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc aattgctcca
    11881 aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt ccctcttaat
    11941 ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca gcaatgataa
    12001 ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt tttaaaactg
    12061 atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt aatcaaagaa
    12121 actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc atctggcaca
    12181 tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg caactggtct
    12241 cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc agtcatgatc
    12301 cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa taatatctgc
    12361 ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat cctagtgata
    12421 tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt gaattgttct
    12481 gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat tcctgttata
    12541 cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca aaatagttct
    12601 tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta ataggcattt
    12661 tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc cattggatgt
    12721 ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg cttcgtactg
    12781 ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac atcaagataa
    12841 gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc tgcacctgtt
    12901 tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc acgggttttt
    12961 gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat acaaagtgcc
    13021 atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat cttatttgcc
    13081 ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt tcaactcaac
    13141 aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg ctacaacatt
    13201 ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta cacttgcttt
    13261 gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg taggaatggg
    13321 tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt cgaagccgcc
    13381 tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct catccatcac
    13441 ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc ctttaaaggc
    13501 attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta
    13561 gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc cgatcgtatc
    13621 tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg ctggatcaac
    13681 aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga tatttccagg
    13741 tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga tgtcaagttc
    13801 ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga
    13861 ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc ctaccaaatc
    13921 cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt caggcacttc
    13981 tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca ctaaagtgac
    14041 aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac gatctttttc
    14101 gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat
    14161 ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg ttaattctgg
    14221 tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag taatctttga
    14281 atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc aagatgatat
    14341 tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat tcatctggat
    14401 gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct aaagcagctg
    14461 tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct accattccaa
    14521 tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca attgcatggc
    14581 caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca tcttcttcca
    14641 ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt tccagaagat
    14701 ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct atagctgctg
    14761 atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt tttagaacct
    14821 caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt ttcgctttgt
    14881 ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg
    14941 gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct gctagatcac
    15001 ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta
    15061 aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc gccggtacga
    15121 ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt tctgcatata
    15181 gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa atcgtttgaa
    15241 gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat ttttggttat
    15301 caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga attaatcgag
    15361 tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca tgcgtgagta
    15421 agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat caggatggat
    15481 ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg gtggtacaag
    15541 ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag ttaatttttt
    15601 catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat
    15661 cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt ctgcagcatt
    15721 aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt tacctgtttt
    15781 accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct gtaggatatc
    15841 gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa tcactttcat
    15901 tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta aaccttggaa
    15961 agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg cgcccccggc
    16021 tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac aaggaccagc
    16081 catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga ctgtattgcc
    16141 aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag cattttctac
    16201 tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt ttagtccaat
    16261 cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt cgatatgatc
    16321 gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta tcattgtttt
    16381 tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt ttcaaggtgg
    16441 ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt
    16501 cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca
    16561 gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat
    16621 gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat agatacaaac
    16681 ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat
    16741 acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa ctgatgtaag
    16801 aattgtacct agtatagaca taaaaaaatc gactgtcaat catttttatg aaactttttt
    16861 taattttctg atagttaact ttgtttttca gaaaaataac aattggtttt attgaagaaa
    16921 atagtcatga acaaaagtat ctgagaactt ttttactctc ttgttcatga catatagaat
    16981 aaagcagctt tgtcttgtaa actccgtttt tcaaataata atctaaagat tgctactgaa
    17041 taataaagcc gatttcatgg tcctcttcgc tgctgattcg gtaaggtgac tcaacatccg
    17101 ctccccagct attgataccc ccgactcctc ttacttttgc aaaaatcgta agaaccgttc
    17161 gtctttctgg cggtagttct tctatatgtg ttgcctgttc cagttcttct gctttgaatg
    17221 gtaaagcgga aaaagcaaat ggcgtatcct gctgttcaaa tcgcaacgaa aaatcttctt
    17281 gatctttatc cgtattatcc aacgtttgat ttcttgttag ttctacccat ttcgtttcca
    17341 tatgcatgcc gcattcttgg ggaacaaggt atggggaaac ggggagtcct gccactttat
    17401 aaataccttc gacaccacca gccatccgat ccggatatgt ttcccctgat aggccttcat
    17461 actcaaaaaa ttttgctttc gttggcataa tgaaccttaa acccaatgcg ggtaattccg
    17521 gtaatccttt ttgtccataa taatgcatat tgacaagcat tttaccctct ccagcgattt
    17581 ggtaggaaat tgtcacttgg gtttttggta ctgtgttgat ttccagtgta aaaatgattt
    17641 caaccgtttc agcgtattca ttacttgaat attgattatt tactggagca atcggcaaat
    17701 cgatcttttt attgtccacc agtacttcta tttctttgac ttttggatac aatccggctc
    17761 ctaaccacat accggattta aatccaaatc cgctacctct gtcattatcg gtcgttgctc
    17821 gccaaaaaac tggaagtgtt tcccggtata gccattcttt tccgttttta ttgagcgatt
    17881 ccagtccgcc tcttgtataa gaaaaaatat aatgcgtatt ccccgatttg acacctaatg
    17941 tacagtcgcc gaagacgatt gcaacttttt ccgtatccat atgactttta tttactgtat
    18001 ttttcataat aatatcttac ctcctgcatg gcgggttttt cttgtccatc tgcaaaaacg
    18061 atcccattcc ctgagaattc atagtcactt ggtttatcgt caaagtcacc gccatagcgc
    18121 atcacttctc tgcctgtgat tggatcagtc acttggatcg cctgatcttt aaaatcccat
    18181 atatagcctc cttggtacat ttcataacga tcaagcaacg tcatgtaaga atccattcca
    18241 cctaacgaat tccccatgtc atgcatatat tcacataaga tgaacggttt ttccgcttta
    18301 ttttcaaggt agtcaatgat ctcttctggt ggcgcataca tgcagctttt tacatctgaa
    18361 atcgaatctt cgaatgctgg atttggaaaa ttcccttcat aatggacaag tcgatcgggg
    18421 tctacttttt tgaagtaatc gttcatttgt cgaatgtttt cttccgcata agattcattt
    18481 ccaagagacc aaaaaagaat acttggatgg ttttttaatg tttcgaaatt cgtttttgct
    18541 cgatcaagaa cagccgcctg ccactgaggt actgcacctg gtacgttgta agaaggctca
    18601 aatgcgccca ttttttgcca tgagccatgg gattcgaggt tcgtttccgc catcacatgg
    18661 attcctgctt ggtcgcataa ataataccaa gtgatctggt tggggtaatg agatgtccgt
    18721 acgccgttga tctttgcatt ttttatgata gccaaatctt tttccatatc ttctatagtg
    18781 atggcgcgtc cttttttcgg atgccactca tgtcgattta ccccgttgat aatcatccgt
    18841 tttccattga agtagatgat tttatctttg atttcgattt tacgaaaacc gaaggaatag
    18901 tcaatatact ccgttactct tttttcttcg tctatcgtct ccaattcaag cttatataaa
    18961 ttgggctgaa aatgccccca cagtgccaca ttttcaaatc catcgatgcg aatcgtcatt
    19021 tgctctttta acggatattg ctgttgatag atttccttcc catcaggatc tagtatcgct
    19081 acattcagct gactgttttt ggctacttgc tcaaaagaca aatctacttc aagtttgcca
    19141 ttttgacatt tgtcatctaa gcgaggtttg atccaaagat cttctaaatg gttttgtcct
    19201 cgagcaacta agaagacatc tctgaagatc ccaaaaaatc ggaaaaagtc ctgatcttcc
    19261 aagaatgcag ctgtacttct tttgtaaacc tccacagcta gaatattttc ttcttccaaa
    19321 taaggtgtta aatcaaattc agacggagtg aacgaatctt cggcataccc gataaaatga
    19381 ccattcaacc aaataaacat tgcttgttct acaccagcaa aatgaatcga gatttgtttt
    19441 ccctttagac cttcttctaa tcgaaaagtc gtcaaatacg aaccaaccgg attgtcttta
    19501 gcagctgaaa agatcccttc cgcctctttc tcagggcttg tcgtgaaagg gggacggcga
    19561 aatacatgtc cctcccacgg atacatcgta ttgatgtaat ggatcttgtc ataatttgct
    19621 aattcaatat gactaggaac agtgatttca tcaaaagaac aggcatcaaa atctttttta
    19681 taaaatgttt ttggtcgtac cgaaggattt ggcgaataac aaaaacgcca tgatccattt
    19741 aaagactgtt taagggagga tgtggtttct acttcttccc ttgttgcata acaaacatga
    19801 tcgctatgtg catttgattg gttcactcta aaaacttgtg gatcatctaa ccattttata
    19861 tttgctttca tacttttttc cttctctctc ataacttttt gaaatgaagc agttctgttt
    19921 ggaaatcacc ttgaaaatcg gggaagtaaa gtccaactgt catcaattcg gcccccgaaa
    19981 atatccgctt cgaggtctgt tcctgataaa gtgcctcatc atctaacccc atcagatata
    20041 cttcatggaa cgctggttga gctgaagcta gtatcctccc taaaaacaca atagcttcat
    20101 cttgtttagg agacacaaac atccatgcgg caatattttc ttcaaaagga ctcgccaaac
    20161 gataataatg gccatactgg atcaccggac gaatcgtttg ataggatata atttggtctt
    20221 ttacttgctt ttcttcttca ggagacagct ttgtcaaatc cagttcataa ccaaaaacag
    20281 cgctcatagc tgtttctcct cttgttttca acgaagtaga tctgtcggtc tgatgatttg
    20341 gtacagctga tacatgagct gtgatggacg aaatcggata agcaaggctt gttccatatt
    20401 ggattttcat acgctccact gcatccgtgt tatcacttgt ccatgactgc ggcatataat
    20461 aaataaaccc ggcatcaaac cgtccacctc caccagaaca gccttcccat aagatttccg
    20521 gataatcagt tgtcaaatgt tcaagtagtt cataaagccc gagcatataa cgatgcgcta
    20581 cttctccttg tctctgtggg tcaagggtaa tcgaatagac atctgaaagc gaacggttca
    20641 tatcccattt gatgtagtca agaggaatcg tatctaaaat ctttctcatt tgtttctcaa
    20701 tcgtctgccg aacatctaac cttgtaaaat ccagtacatg ttgtgatctg gaagcagaag
    20761 gcattctacc tggctcctgc ataagaaaat caggatgtgt tcgatacagt tcagaatcaa
    20821 ccgagatcat ttcaggttca aaccataaac caaatttcaa tcctttgcta tgagcataat
    20881 ctgctatttc acgtaatcca tttgtcaact tgccttcata ctcgaaccag tctcctaatg
    20941 aagaattgtc cgcatctctt tttccaaacc agccatcatc taatacgaac atttcgatgc
    21001 ctagctcctt tgcttcatca atgatcgcct tgattttttc actattgaag tcaaagtaag
    21061 tggcttccca gttattgacc agaatcggcc gttcagcaaa ttgatgacga cctcttgcga
    21121 ttctttcacg tagcaactca tggtggatct gactcatttt gttcaatccc tgacttgagt
    21181 aagaaaggat catttcaggt gtttgaaaag actggcctgc attcaattcc cacgaaaaac
    21241 gatggctgtt gatcccaccg acgactctca attgattgat ctgatctttt tcaagttcga
    21301 aagaatggtt gccggaataa acaaattgaa gtccgatcgc ttctccagta ttttctgtcg
    21361 tatgatgatg aactaaagca ataaagttat tcatatggtg actgctcgtt cctcgacggc
    21421 tttcaaatac ttttgttcca gataaaacag attgccgtga aatctgtctt tcattgacat
    21481 gtgctcctgg caaagcaatg acttcatcaa atcgcctagt atgggcaaag tctagctgga
    21541 aactagccgc tttttctagt ttgattgttt ctccggtttc atttcttatc tttacagatc
    21601 gtgtgatcac tgctctatca gtaaatatgg tatagaacaa atcgaaataa atcgctgttt
    21661 ctctatcctt aagtgaaatc accaacgttt gtgcctctga cttgtccatc acataagttt
    21721 gaggcaagcc ttcgatctct ggcttcccat ctgtaatata atggctgtca tatctcagat
    21781 ccagtaagtc tgatccattc ttgcgctgaa tgatcgatgc gggcgttcga aagtccatgc
    21841 tgccatgaga agagtattct tgcggtagtg tatctttcga cagagaccgg tcaggattta
    21901 agggaacatt tcctgaaaat ccacgatctc ttcggggata atttttattg tctttgtact
    21961 gcttgactgg tttgccaaaa taaatatgcg ccaatacatt gctttcttct atctttatca
    22021 gataagaaag aaatgtattt cgtaaatgga agactcggct cttttcatca aataagatga
    22081 aatttttatc tgtttttcta tgcgtaatat ccatcatttc actcccaaca aaacggaaac
    22141 cgtttacttt tttttataat tcctgtatac tgtttactaa acaattttgt caataaaaat
    22201 tttttatttt tttgaggagt gtgtaaaaat ggttggaatt agagatgtcg cacgaaaggc
    22261 aaatgtctct cctgctacag tttcccgtgt gttgaataac gatcagacga tccatgtggc
    22321 tcagactact cggcaacgga tagtagaggc agctgctgcg ttggattatg acatcagtac
    22381 tcgtaaatat atcaaaaaga gaatgccttc gattggtgtg atctctacga tcggcaaagc
    22441 gagcgaagaa caggatatct attacaaaca tttgcgagta gggatggaag aagaagctcg
    22501 acgtctgcat ttagggatga atcgaatcta taatctatct gataatccta aacaatggga
    22561 agatctagat cagttaggtg cggtgatcgt tgttgggact gttacgaaag aatcgattac
    22621 cgccttgtta aaacaaaaca tgcaaatagt cgtcgtggat aaccctgaca tccagcaaga
    22681 ggtcgatatg gtgtatgcag atcttgaacg gatgactaaa accgtactgg aattgttctt
    22741 agaaaatggt cataaaagaa tagcctatat cgggggatat aaagtcgata tggatgaatt
    22801 cggtgggaag cagatttcta taaatgaaaa gaggatgcgt gcctatcagc attttatgat
    22861 cgatcatcac ttagaaaaat atatgtctta tcatctaggt ggctggacag aaaaacaagg
    22921 tgaggtactt gcgactaatc tattagaaaa tgaatcccct cttcctactg ccattcttgt
    22981 cgggagcgat ccaatggcaa ttggtgtcta tcgggcattg aaacaaaaaa atatccaaat
    23041 tggagaagag attgttgtag caagctttga taatatcgag acaatcgcaa acctctcccc
    23101 tggcttgacc agtgttcaaa tcaatgcgaa atcaattggg caagcagctg ttcgaatggc
    23161 attggagcga attgacggta tacgggacga accaatcatt ttgacttatc ctgcacgatt
    23221 gattgttagg gaaagtttta tcccaagaac aaaaaggtat tgaaagagag gttgtgaaaa
    23281 gccgcttcgt ttcttcatac tcaaaacgaa acggcttttt cacaacctct tacttatact
    23341 tattcacaaa atccaatgtc tgctggtaac aattttgcga ttcttttagc gtgtaatcaa
    23401 attggtattc atgggtaatc tctttttttg tatctgtata aaacaaggat tgcacgggta
    23461 cctttaattc tgtcaaccga ttttctaatg cttgaccttg ctcctggaat gagtatgcat
    23521 ttccgtcagt tatatacgtt ggcggaaatt ccttagaaac atggtcagcc acactagctt
    23581 ctttcaattg ctcactcgtt ttccaatctt ttgttccaat caacgaccac gcaacggttt
    23641 tgacgaaaaa cttcataaat cgatcatccg aatgctgctg tgctgttagc ttcaaatcaa
    23701 caggtccgca ataagaaatg gttcctttca gactatcttt tggaagtagc tgtttgattt
    23761 tcatttgttt agcatacgca tcattcgttt gtatagctgc atattgcaat gcgatttgtg
    23821 aacctgcact atctccacca aaaaataatt gttccatgtc tagcaatttg tcgtcttttt
    23881 cttcagctaa taaatctttg atcagttcat tcacttgaag aacttgatta gggtattctg
    23941 aatcaggcgc taattcataa ttcatcgcaa ccactgcgat gttggcgtct gaaactaatt
    24001 ttgtcgcaaa ttctttgaca cccgatttat ctccgccgac gaatcctcca ccatgtaccc
    24061 aaaatagaac aggaacaggc cccttgctgt tttttggata gtagatgtca taagtattct
    24121 ctttatactt ggactcatac gtcaaatctg tttttttcgc cacgtttttt tctgcttgat
    24181 cgaacgtttt tttatcagtg atttgaacag tatttgaaaa catatggttg atcaagaatg
    24241 ctccaggtct gggggaaagc tggaaggcaa gaaagattgc aaacgcgatc ccaagaatga
    24301 ttccgaagaa ccatccgata aatttaagaa ttttcttttt cataaggctc cctcagtagt
    24361 tagtattaag cattctgatt ataccatagt gttatttcct atcaagctct cctagatgat
    24421 ttaatgattt tccccaaact tcttctgcaa tgcttttgac aaaagaaatt ttttccattg
    24481 ttcttcaaac aaaacatttt cttcttctgt tgaagcaaat ccacattgag gacttaagca
    24541 caaacgatcc aaaggcagat atcgtgtcgc ttcgtagatc cggtcaatca cttgctggtg
    24601 ctcttccatt tcgccatttt tcgttacagt caaaatctaa gacaactaat ttatcttcag
    24661 agactttctc taaaagaaca aaactcccag cacgttcact atcgtattct aaataatagg
    24721 cacggacatt ttcttgagta aaataatggg tctgctacgt cgtcatcccc cctgatgccg
    24781 ccaaaatcga ataataattt ccgcgtcaaa catgtatatt tagtgttgaa tcatttgaga
    24841 atatgaaaat aaaaatcttt ttgatcaatc agttaatcaa attgaaataa cgaaaaatct
    24901 gagacagaat gacgttcatt ctgacccaga tttgattttg cacaactcaa cagtccttat
    24961 tggcaaatcg ccaaaataat gtagactaac taaaaagact aagaaacaat aacaaaagtt
    25021 aacacttctt agtctttctt tagcacgtct atttattaaa gattttaggg ccttttctca
    25081 tgtttttacg atcattctta ggtgtttcca tttgtttgga tcggcctcca ccttgtttct
    25141 ttttgatcaa ttctttcatt ttttcttttt ggtccatttt tattctcctt taacgaatga
    25201 agcgactaat tcgatatgat gggtttgcgg gaaaagatct acaggctgaa ctttcttcac
    25261 ggcataacct gcttctgcaa ataacttagc atctcttgca aaagttgccg gattacatga
    25321 gatatagacg attcgttctg gtgacatttc gattgctgat tcaataaaat gatgatctaa
    25381 tccttttcgt ggagggtcaa ctacgatcac agatggtttg atcccttttt cttgccattt
    25441 tttcataact ttttctgctt ttccgacttc ataatgaaca ttttcgattt tattatttaa
    25501 agcattgaac tgtgcatctt caattgcttc tggaataact tccatcccat acaccttatc
    25561 cactctgtct gctaaagaca agccaatcgt acctattcct gaataagcgt cgatcacaat
    25621 gtctttttta tctaactcgg caaactcgat ggctgtttgg tacagatact ctgcttgttg
    25681 agtatttact tgataaaatg actttgctga gattcgatag gtttttccta atagctgatc
    25741 ttcgatatag tctctgccat ataaaacttt ttctttgtct cctaaaatca catttgtttt
    25801 ttcttcattg atattttgga tgaccgaaac cacttctggc aattcttcat gaatagcttc
    25861 agcaatttcc ttggatttaa agaatttttc tttacgagta actaaaacga ccatcatttc
    25921 atgggaataa tgaccacgac ggataatgat gtgacgaagc tgtccttcat ttttttcttc
    25981 attatatcct cgtacttgga accgttgcaa gatatctctg actactttga ttgcttgatc
    26041 gatgacagga tcttggatat aaaaatcttc gatagggact agatcatggc tattcttttt
    26101 gtaaaaaccg gtggctaact gtccatcgat acggcgaaca gggatctgtg ctttattacg
    26161 gtagccgaat ggttctgcca ttccgaccgt tggaagtaca ggaacttctg gcatcttagc
    26221 aatcttcgcc attacatgct ctacttgttt ttgtttgaag acaagctgtt gttcatactt
    26281 caaatgggct aaaggtgcga tacctatgcg aagtaatgca ttgttttcta caggctgacg
    26341 atcaggtgat tgtgtaataa atgtttcgac ttttccatat ccaaacttat tgcctgcttt
    26401 tactactagt atttctactt cttctccagg gagtgcattt tcgataaata atggataacc
    26461 atcgattttt gctaccccca ttccttcata actcaagtcg acgatttcga ctgtatatcg
    26521 ttcgtttttc tttactattt gctgttctgt catattttgc tcctttaagc ctatttttca
    26581 atcctactga actctactat aaatcagcta attacacaac taaaaccggc actgacctta
    26641 ctacgttcag caccggcttt tgattaattc ttttgattag gtgtttcgtc tgctattttt
    26701 ccgtcaccgt cgatatcttc atccgttaaa cgttcgactt cttttacgaa ttctttgctg
    26761 acaacctcta attcttcttc atctgtacct gtgattgctt catcaggaat ctgatcggta
    26821 ttggcaaaga actcaatgtg ttgatgaaga ttttggaaat gcatcggtgc ttccccgccg
    26881 tattcaccat ctaagttgat cattactggt cgattgatat catcttctgt ttcaacgaac
    26941 aaactgcttg ttttggtata gattattctt ggatcatcga catgtcgacc gccattcagc
    27001 atgagtgctg caatatgcaa gatctcaaag atatttgctg tcttcacaat gattaaagaa
    27061 aacttcccat catccaattt ggcatctggc gcgatctgtt cgaatcctcc aacagagttc
    27121 gtcagcccta ggaaaaacat cgaagcattt ccgatatatt ccccttcatc atacttcaag
    27181 cgcattttga ttggctttac tcgagggagc atctctgctc ctttcgcaag ataagcaaga
    27241 taaccaaaaa tactctttag ttctgatgga acttcataag tcaattctgt caaatagcca
    27301 cctgcagcga tattgatgaa gtaactgtta tcagaaaggc cgatgtccat ttttacggtt
    27361 tggtgttttt ttatcacttc tgctgctgct ttaatattat ctctaggaat ttttaatgct
    27421 cgagcatagt cgttcgttgt accagctggg atgatggcca ttttaggacg gtgttcaagc
    27481 ggagcgattc cattaaccac ttcgttgatt gtgccatcgc caccagctgc cactactaga
    27541 tcaaaaccta gttctgcgac tctacatgct tcattcttgg ctgaatctgg ttctggtgta
    27601 gttgcatagg cgctggcttc aaatcctgct tcttctaatg cctgtaggat atccgccaaa
    27661 ttttttttca ataattcttt tcccgaggtg gggttataaa ttacgcgtgc ttttttcatt
    27721 ttcagcctct atcgtttcgc taattcatct ttaagtagct tattcacgac accaggattt
    27781 gcttgtcctt tagttgcttt catgatttga ccgactagga agccaaccgc acgatctttt
    27841 ccatttttga aatcatcgac agattgttgg ttgttatcca aaatttcatt aatgattggc
    27901 agtagtttgt tcggatcaga taattgcacc caaccatttt tttcaactac ttcttgggct
    27961 tctccgccat ttgtgatcaa ttcacggaag acttttttgg cgatttttga gctgatcgtt
    28021 ccatcggcga tcaaacgaat cattcctgct aagttctcag gtgtcaattt tgtctcagct
    28081 aattccagtt tctcactgtt caagtaagca gaaacttcac ccatcaacca gttagatgct
    28141 tgcttggcat ctgcaccatt ttccaaaaca gcttcaaaaa aatcagacat ttcttttgta
    28201 agtgtcaata ccattgcgtc atactctgga agtccaaact cacggatata gcgttcgcga
    28261 cgagaagctg gcatctcagg caaactagct tttacttttt gaatccattc gtcatcgatc
    28321 acgaatttcg ggatatcagg ctctgggaag taacgataat cactagaccc ttctttgaca
    28381 cgcatcaaaa ctgttttatt cgttgtttcg tcaaaacggc gtgtttcttg ttggatttct
    28441 cctcctgaaa gaagaacttt tgcttgacgt ttttcttcaa aagctaaacc ttttttcacg
    28501 aaagataaag agttcaagtt tttcaattct gctttcgtac cgaactcttc ttgtccataa
    28561 gggcgaagag aaatattggc atcacagcgc atagagcctt cttccatttt cacatcgctg
    28621 acttctgtaa attggataat tgatcgcaat gcttctagat aagcgtatgc ttcttctggt
    28681 gaacgcatat cagcttcaga aacgatttcg atcaaaggcg tgccttgtcg gttcaaatca
    28741 acgtaagaat aaccgccaat accatgcatg ttttttccag catcttcttc taaatggaca
    28801 cgttcgatcc ggattttttt ctttttacct tctacttcga tttcgatcca tccgtcatga
    28861 ccgattggtt gatcaaattg ggaaatttga tatgcttttg gattatctgg gtaaaagtaa
    28921 tttttccggt caaaatgagt atctttagag atttcacagt ttaatgccaa agctgctttc
    28981 atcccgaatt ccagcgcagc tttattcatt actggcagca cgcctggata accccagtcg
    29041 atcacgtttg tattgctgtt tggttctgca ccaaaatgag ctggcgctgg agagaaaatt
    29101 tttgagttgg tttttaactc gacgtggact tctagtccaa tgactgtttc aaaattcatt
    29161 cgtctttccc tcctaaaata gctggttgtt gtttatggaa ttctgttgct tgttcaaacg
    29221 catatgccgc ttgatacatc gttttttcat caaaataatt accgatgatc tgcaatccaa
    29281 ctggtaatcc ttctgataaa cctgccggta cagacatacc tggaagtcca gccaagttga
    29341 cagggatcgt taagatgtca ctcatgtaca ttgtgattgg atcgttaatg ttctcaccga
    29401 ttccgaatgc aactgttggt gaagatggcc cgatgatcaa gtcataatct gcaaagactt
    29461 tttcaaaatc tcgtttgatc aatgtacgga cttgtcctgc ttttttgaag taagcgtcat
    29521 aataacccgc gcttaaggag aatgtaccta acatgatacg gcgttttact tcttcaccga
    29581 atccttctga acgtgagttg acatatacat catctaatga tttcacattt tcagagcgat
    29641 aaccgtagcg gataccatcg aatcgttgca aattggaact tgcttctgat gaggcaatga
    29701 tataataaac agccacaccg tattttgaat gaggcaagct gacttcttcc acaacagcac
    29761 ctaatgaacg gaatgtttct gctgctttca atacagcttc ttttacgcca ggggccacac
    29821 cttcgcctaa gtattcttta ggtaaagcaa tcttcattcc tttgatgtcg cctgtcagtc
    29881 cctctgcaaa atcaggaact gaaacgcctg aagaagtacc gtctttttcg tcataaccgc
    29941 tgatagctgt caatgctaat gcattatctt tcaccgtacg ggtcattggt ccgatttggt
    30001 caagagatga agcaaaggca atcaaaccaa aacgtgagac acggccataa gtaggtttca
    30061 tccctacgat gccgttaaaa gctgctggtt ggcggatact tcccccagta tcactaccta
    30121 aagaaactgg aatttgtcca gctgcaactg ctgctgcaga accaccagaa gagccaccag
    30181 gtacttttgt tgggtcccaa gcattttttg ttttcttgaa ataagaagtt tctgtacttc
    30241 cacccatagc aaattcatcc atgtttagct taccaacagg gatcatatct gcttgataga
    30301 ttttatccat gaccgttgca tcataaattg gttcaaaatt gtaaaggatc ttagatgcag
    30361 cggttgtcaa gatatctttt gttacgatat tatccttgat accaattgga ataccagcaa
    30421 ggacgttgct ttcatcaatc cctttttcat ctaaagcttt tgcctgttcc aatgcttttt
    30481 cttcgttcaa tgtaatgaat gcctcgacct caggctctgt ttctttgatt cgttcaaatg
    30541 ttgtttttgt caaatcttgt acagtgattt ctttcgatac aagcatatga tgcagttctt
    30601 ctagagaacg attgtgtaat tctgtcatta tgcgccagcc tccccattat cgatgattgc
    30661 gggtactttg ataaagccgt cttctgtttc cggcacattt ttcaataatt catcgcggtt
    30721 ccatccagct tgcgcccgat cttgtcgcat cacgtttact gtttcaacca cattcgacgt
    30781 aaatggaaca ccttccgtat ctacttcttc cagcatttca accatatcga tgattttacc
    30841 taattggtcg gtaaaacttg caagttcttc atctgcgaac gcaagttttg ctaatttggc
    30901 aacgtgtttc acttgttctt cactaatcgc catactaccc cttctttcta aacgagttaa
    30961 aaactttgct ctctatttta acacaagagt gccgtgtctg aaattgttta ttgaaatttg
    31021 tctgaaaaaa ctcatttttc attcttctac tgagcgactt ttagccaatg catctgccat
    31081 ttgatcttct gtccaaactt cgattcctaa ttcttgtgct ttggtcagct tgctgccagc
    31141 atcttctcct gctaccacga tatccgtttt tttcgatacg ctaccagtca ctttcccgcc
    31201 aagattttcg atcgtctctt ttgcttcttc acgagtaaat cgtgttagtt taccagtaag
    31261 gactactgtt ttttctttaa aaggagactc gacttcttgc aattgggcat ttcgaagccc
    31321 tttgtattcg aaattgacgc ctgcttgttt taattcattc atcaattcat ggacttcttc
    31381 gttactgaaa taagtgacta cactgtcggc aatgatatca ccgatcgtat caagcgcaat
    31441 aatcgactca tagtcacttt tactcaatgt ttctagatca ccgaaatgtt ctgctaaaat
    31501 ttttgctgct ttcgctccga catgtcggat tcctaacccg aaaatcaacc gctcgaccga
    31561 attatctttg ctgttgtcga ttgctgtcaa aatattattt gctgattttt cttttatttt
    31621 gtccaacgtc aataactctt cttctgtcaa tttgtaaaga tcagcaacat ctgctactag
    31681 ttttttatca tacatctgtt ctagtacgcg cggccccaac ccatcgatat tcatcgcatt
    31741 tcttgaaaca aagtggttca aaccttcttt catttgagca gggcatttag gattgatgca
    31801 ccgtaaggct acttcttcat ccaaatggac aagttcactt ccacaaactg gacaatgtgt
    31861 aggcaattgg tattcttcgc tgtctttagg tcgtttatct aacacgactt gcgaaacttc
    31921 tgggatgatg tctcccgctt tataaatcag aactgtatct ttcaagcgga tatccttcaa
    31981 cttaatataa tcaccgttat gcaggctggc acggcttact gtcgttcctg ctactcggac
    32041 tggtgtcatg atagctgttg gcgttactac ccctgttcga ccgactgtcc attcgatgtt
    32101 ttcgattagc gtttcaactt cttctggcgg aaacttgtaa gctgctgccc atctcggtgc
    32161 tttgatagta aaaccaagtt gatcttgtag cgagaattca ttcactttga tgacgattcc
    32221 atcgatttca tagggcaaat ccacacgttt gtcatgatat tcttcaatgt atgaccacac
    32281 ttcatcaatc gaatgacata gacgtttttc ccgattcgtg tggaatccaa ttttttctag
    32341 ctcttctagc gcatcatact gggtttttgc ttgcatagga ccgaaatcag ctactgtgta
    32401 taaaaaagtg tccagatttc ttttcgctgt gattttagaa tctaactgac gcaaagaacc
    32461 agctgcggcg ttccgaggat tcgcaaagat atcttttccc tctgcttctc tgtcttggtt
    32521 caattggaca aatgaccgtt ttggcatgaa acattcgcct cttacttcga tgttcattgg
    32581 ttcttttaat ttgattggga ccgaccggac agtttttagg ttttcagtga tgttttctcc
    32641 tacagttcca tccccgcgag tcgcacctct cacgaaattt ccatcttcat aacgcaaaga
    32701 aacggaaagt ccatctattt ttagttcaca gcaatagtca actacacgtc ccactgcttt
    32761 ttgtacacgt tgatcaaaag ctatcagctc ttctttgctg aaaacgtcgt tcaaactgta
    32821 aagcggaata tcatgagtga ctttttcaaa accttctaat acttttccgc ctactcgttg
    32881 agtgggagaa tcagacgtga tcaaatcagg atattgttcc tcgattgcta ctaattctgc
    32941 gtattcttta tcataaacat agtcttccac tgttggttta tcttctacgt agtattcgcg
    33001 agaccattgg ttcagtcttg tacgaagttc ttctgctcgt ttagcagctt cgtcaatcgt
    33061 catttcatgt tccatcattt accctccact actaagcttt ttcaattggc gcaaaggcag
    33121 ccaaaagacg ttttactcct tgacttggga aagccacatc tagttctaga tccttcgacg
    33181 tcccgcttac tcggacgact gttcctagac cccatttttt gtgtttgact ttatctccag
    33241 gtttccagct gttattttcc cctccagtag cacttttaga gggaacaacc gctgtctctg
    33301 gctgtttata gctatatgct gtttttttag cgaatacagg cgttttttta ggcgtaggac
    33361 gcatgccttc gatttccagt aactcctgat cgatttcttc tacaaaacga gaaggtcggt
    33421 tatattgcgt ccgtccatat aatgttctgg agaaagcatt tgttaaatac aacgcttctt
    33481 ctgctcgcgt gatccccaca tatgcgagac ggcgttcttc ctccagttcg ctttcttcca
    33541 tcaatgctcg agataaagga aaaaccccct cttctagacc aatcaggaat acaactggaa
    33601 attctaatcc tttggctgca tgcaaagtca tcaatgtcac ttgggaagca tcttcttcta
    33661 aattgtcaat gtcggaaaca agtgccagat catttaagaa gaccgttagt ttttcttctg
    33721 gtgcatcagc gtcttcttca ttttgctgtt cgaattgttt gtcgaattct tgtgtgaccg
    33781 ttaaaaattc ctccaagttt tcgatgcgtg cttgtgcttc taatgtattt tggattttta
    33841 agtcttctag atatccgcta cgatccaaaa cctctttcgt caattctgtc actgtcaagt
    33901 atgggatcat ctgagtgact tcttggatca tctctccaaa tgcacctaat tgctgtcctg
    33961 cttttccacc gatatttgcc agatccacat tttgcgctgc ttcaagcagt ggccattcat
    34021 gaagagaagc aaaactgcgt agtttttcga tagatcctgg tccgatgcct cgtttgggcg
    34081 aattaacgat acgctcaaaa ctgattgaat cttgaggatt taccagcaca ttcaaatacg
    34141 ctaaaatatc tttgatttct ttacgatcgt agaacttgtg ccctccaacc attttataag
    34201 gaatatttgc tttcaacaga gtttcttcca ttacacgaga ttgtgcgttc gtccggtaca
    34261 agatggcaaa gtcgccgtaa ttccgatgat tgcttcgaat ctcttcttgc atccggtcta
    34321 cgataaaacg ggtttcgtct cgttcattat ctgcacggta gtaagtgatc ttgtttcctt
    34381 ctttgttttc tgtccacaag ttttttggtt ttcgattgct attgttttca atgacttggt
    34441 ttgctgcgct aaggatgttt ttcgtggagc gatagttttg ttctagcaaa attactgcag
    34501 catctggata atctttttca aaatccaaga tgttctgcat atctgcaccg cgccagccgt
    34561 aaatactttg atctgcatct cccacgacgc acaggttcct aaatctacct gccaataaat
    34621 tgactagcgt atactgtgcg tgatttgtat cttggtactc atctacatga atataatgga
    34681 atttgttttg ataatacgtg agtgaatcag gatgttcttc aaataatcga atcgtattca
    34741 tgatcaggtc gtcaaagtcc atacattgat tatattgaag ctctttttgg taagcagcat
    34801 aacattttgc cgcaatctct tcgtaataac tgccttgcat ttttgcaaaa tcctgagggg
    34861 tctgcaggct gttttttgct tgagagattg ttcctaatat acttcgagga tcatattttt
    34921 tcggatcaat gttcagttct tttagtatcc gtttcatcaa ggtcagctgt tcagaagaat
    34981 cgatgattgt aaagttccgg ttatacccga taaaatcaac atctcttctt aaaatacgca
    35041 cacacatcga gtgaaaagtc gatacccaga catcttcgcc tccagatgcg agaatcgcat
    35101 tgacacgttc tttcatttct tttgcagcct tattggtaaa agtaatcgct aggatgttcc
    35161 aaggattgac ttctttttcc tcaatcaaat aggcaatgcg gtgtgtcagc actctcgtct
    35221 ttccacttcc tgcacctgcc attagcagta atggtccgtc agtgtgaaga acagcttctt
    35281 tctgacgggg gttcatcccg tttaataatt cagctttgga tgacattaac aacatccttt
    35341 cttttatcaa tcttgttaat tatagcattt tcttggcttt cattaaacta gctagttcaa
    35401 aatcatttgt ttctttttat ggatttttcc ttgtcaattc gttgatttcg cgctaaatta
    35461 aaagaaagcg aggcgtttga atggaaaaaa aaggaaaaat caagaaatta gataagactc
    35521 agatatattt tattctaaaa ggaatatttg ttggactgct gacaggaatc gtcgttagcc
    35581 tgtttcgatt atctatcgaa aagctaagcg atttagttcg aagtatctat gaaatgagtc
    35641 gggaacagcc gatttactta attggcctag ctatttgctg catccttgca gcatttttcg
    35701 tgggttatct ggtcaaaaat gaaccggata tcaagggaag cgggattcca caagtagtgg
    35761 gacagcttcg cggggaatta tcaatgaact ggttctctgt tttatggaaa aagttcattg
    35821 gcggtgtact ttcagttggt gcgggccttt ttctaggtag agaaggacct tctatacagc
    35881 ttggcgcatc cattggacaa ggagctggac aattgtttcg gagtccatct tcagaagaaa
    35941 agattttaat ttctagcggt gcaagtagcg gacttgcagc agcattcaat gctcctatcg
    36001 caggattact ctttgtttta gaagaaatcc atcactcctt ttctccactt gtttggctga
    36061 catcatttgc ttcagcaatc actgctaact tcgtctctct ttactttttc ggcttgagac
    36121 ctgttttata tctaggtgac cttccttctt tgccgttgaa atattatggt tccttagtga
    36181 tcttaggagt acttctaggc atcttaggtt ttatttatca gaatgtcttg ctggctttgc
    36241 caaaatttta caaccgattg cctttaccag cttattttca cgggttcgtt cctttcttgc
    36301 tgattttgcc aattggttat ttatggccta gtactctagg cggaggcaat caaatcgttt
    36361 tagcttttgg acatgcttcc ttgccaatca ttatgattat cggtttgttt gttcttcgat
    36421 tcgttttttc catgatttct tacggtgggg gtcttccagg ggggattttc cttccgatat
    36481 tgacattggg ggctttaatc ggtagctttt atggaaacat tatggttgat ttcgggatgg
    36541 atccaatcta catcaaggat tttgtcgtta ttgccatggc tggttatttc acagcgattg
    36601 gaaaagcacc gctgacagca attattttag tgacagaaat ggttggctct cttaatcatc
    36661 tgatgccttt aggattggct gctcttgttg cttatatcgt caatgacctt ctcggcggta
    36721 atccaattta cgaatcatta ttggaacgtc tacttggaaa tactcagacc gcctcactaa
    36781 aaggtgcaag gacaactttt gattttccag taacggcaga gagttcttta gacggattaa
    36841 tggtacgaga cttcaattgg cctaaaaata tgctgctaat ctctatacgc agaggaaatc
    36901 aggaaatctt gactcacggt gatacgatca tgaatgtagg agatattttg gttatcttga
    36961 ctgatgaagc aaacttgcca agaatcaagc aggaaatcga ggctaaatct tctatcacaa
    37021 atttgaagca atcattttaa cgaacatgga aaaactattt tacaacaact attcatctac
    37081 actacttgat tctatatgat tctataaaaa
//
LOCUS       DAZKOU010000018        35943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000018 DAZKOU010000000
VERSION     DAZKOU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.018"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..491)
                     /locus_tag="ACG827_000856"
     CDS             complement(24..491)
                     /locus_tag="ACG827_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483018.1"
                     /translation="MNYLIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFL
                     VKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLL
                     SPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            650..967
                     /locus_tag="ACG827_000857"
     CDS             650..967
                     /locus_tag="ACG827_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483019.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            900..1379
                     /locus_tag="ACG827_000858"
     CDS             900..1379
                     /locus_tag="ACG827_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFQ0483020.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            1396..1572
                     /locus_tag="ACG827_000859"
     CDS             1396..1572
                     /locus_tag="ACG827_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483021.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            1829..2260
                     /locus_tag="ACG827_000860"
     CDS             1829..2260
                     /locus_tag="ACG827_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483022.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            2266..2583
                     /locus_tag="ACG827_000861"
     CDS             2266..2583
                     /locus_tag="ACG827_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0483023.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            2702..3388
                     /locus_tag="ACG827_000862"
     CDS             2702..3388
                     /locus_tag="ACG827_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0483024.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            3363..4712
                     /locus_tag="ACG827_000863"
     CDS             3363..4712
                     /locus_tag="ACG827_000863"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0483025.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            4703..5176
                     /locus_tag="ACG827_000864"
     CDS             4703..5176
                     /locus_tag="ACG827_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0483026.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            5178..5678
                     /locus_tag="ACG827_000865"
     CDS             5178..5678
                     /locus_tag="ACG827_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFQ0483027.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            5693..8005
                     /locus_tag="ACG827_000866"
     CDS             5693..8005
                     /locus_tag="ACG827_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFQ0483028.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            8252..8569
                     /locus_tag="ACG827_000867"
     CDS             8252..8569
                     /locus_tag="ACG827_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFQ0483029.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            8566..8727
                     /locus_tag="ACG827_000868"
     CDS             8566..8727
                     /locus_tag="ACG827_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0483030.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            8730..9029
                     /locus_tag="ACG827_000869"
     CDS             8730..9029
                     /locus_tag="ACG827_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483031.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            9029..9343
                     /locus_tag="ACG827_000870"
     CDS             9029..9343
                     /locus_tag="ACG827_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0483032.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            9336..9866
                     /locus_tag="ACG827_000871"
     CDS             9336..9866
                     /locus_tag="ACG827_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0483033.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            9863..10132
                     /locus_tag="ACG827_000872"
     CDS             9863..10132
                     /locus_tag="ACG827_000872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483034.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            10062..10265
                     /locus_tag="ACG827_000873"
     CDS             10062..10265
                     /locus_tag="ACG827_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFQ0483035.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            10262..10549
                     /locus_tag="ACG827_000874"
     CDS             10262..10549
                     /locus_tag="ACG827_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483036.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            complement(10582..10833)
                     /locus_tag="ACG827_000875"
     CDS             complement(10582..10833)
                     /locus_tag="ACG827_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483037.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            11036..11449
                     /locus_tag="ACG827_000876"
     CDS             11036..11449
                     /locus_tag="ACG827_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0483038.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            12069..12233
                     /locus_tag="ACG827_000877"
     CDS             12069..12233
                     /locus_tag="ACG827_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483039.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            12230..12460
                     /locus_tag="ACG827_000878"
     CDS             12230..12460
                     /locus_tag="ACG827_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483040.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            12645..13190
                     /locus_tag="ACG827_000879"
     CDS             12645..13190
                     /locus_tag="ACG827_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0483041.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            13287..13508
                     /locus_tag="ACG827_000880"
     CDS             13287..13508
                     /locus_tag="ACG827_000880"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0483042.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(13665..13829)
                     /locus_tag="ACG827_000881"
     CDS             complement(13665..13829)
                     /locus_tag="ACG827_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFQ0483043.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            13931..14452
                     /locus_tag="ACG827_000882"
     CDS             13931..14452
                     /locus_tag="ACG827_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0483044.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            14439..14879
                     /locus_tag="ACG827_000883"
                     /pseudo
     CDS             14439..14879
                     /locus_tag="ACG827_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(14919..15077)
                     /locus_tag="ACG827_000884"
     CDS             complement(14919..15077)
                     /locus_tag="ACG827_000884"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFQ0483045.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            15170..15931
                     /locus_tag="ACG827_000885"
     CDS             15170..15931
                     /locus_tag="ACG827_000885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0483046.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            16118..17470
                     /locus_tag="ACG827_000886"
                     /pseudo
     CDS             16118..17470
                     /locus_tag="ACG827_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            17486..18706
                     /locus_tag="ACG827_000887"
     CDS             17486..18706
                     /locus_tag="ACG827_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0483047.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            18741..19418
                     /locus_tag="ACG827_000888"
     CDS             18741..19418
                     /locus_tag="ACG827_000888"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0483048.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            19415..20608
                     /locus_tag="ACG827_000889"
     CDS             19415..20608
                     /locus_tag="ACG827_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0483049.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            20623..20949
                     /locus_tag="ACG827_000890"
     CDS             20623..20949
                     /locus_tag="ACG827_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0483050.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            20953..21288
                     /locus_tag="ACG827_000891"
     CDS             20953..21288
                     /locus_tag="ACG827_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0483051.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            21285..21680
                     /locus_tag="ACG827_000892"
     CDS             21285..21680
                     /locus_tag="ACG827_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0483052.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            21677..22048
                     /locus_tag="ACG827_000893"
     CDS             21677..22048
                     /locus_tag="ACG827_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483053.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            22064..22624
                     /locus_tag="ACG827_000894"
     CDS             22064..22624
                     /locus_tag="ACG827_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0483054.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            22749..23060
                     /locus_tag="ACG827_000895"
     CDS             22749..23060
                     /locus_tag="ACG827_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483055.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            23084..23275
                     /locus_tag="ACG827_000896"
     CDS             23084..23275
                     /locus_tag="ACG827_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483056.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            23275..27123
                     /locus_tag="ACG827_000897"
     CDS             23275..27123
                     /locus_tag="ACG827_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0483057.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            27124..27996
                     /locus_tag="ACG827_000898"
     CDS             27124..27996
                     /locus_tag="ACG827_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFQ0483058.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            27993..29042
                     /locus_tag="ACG827_000899"
     CDS             27993..29042
                     /locus_tag="ACG827_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483059.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            29046..29870
                     /locus_tag="ACG827_000900"
     CDS             29046..29870
                     /locus_tag="ACG827_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483060.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            29888..31948
                     /locus_tag="ACG827_000901"
     CDS             29888..31948
                     /locus_tag="ACG827_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0483061.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            31965..32114
                     /locus_tag="ACG827_000902"
     CDS             31965..32114
                     /locus_tag="ACG827_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483062.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            32111..32557
                     /locus_tag="ACG827_000903"
     CDS             32111..32557
                     /locus_tag="ACG827_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483063.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            32559..32696
                     /locus_tag="ACG827_000904"
     CDS             32559..32696
                     /locus_tag="ACG827_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0483064.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            32731..32973
                     /locus_tag="ACG827_000905"
     CDS             32731..32973
                     /locus_tag="ACG827_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFQ0483065.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            32989..33186
                     /locus_tag="ACG827_000906"
     CDS             32989..33186
                     /locus_tag="ACG827_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0483066.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            33197..34213
                     /locus_tag="ACG827_000907"
     CDS             33197..34213
                     /locus_tag="ACG827_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0483067.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            34328..35536
                     /locus_tag="ACG827_000908"
     CDS             34328..35536
                     /locus_tag="ACG827_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483068.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            35520..35690
                     /locus_tag="ACG827_000909"
     CDS             35520..35690
                     /locus_tag="ACG827_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483069.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT        12664 a         5959 c         7871 g         9449 t
ORIGIN      
        1 attattaaag aaaaattttt caattaaaat gagtctttta gctttaactt aaattttttt
       61 tgataattag ctgttcttat gaccattaca agtttttcac caggtaatat atctttcata
      121 tctaaaactt ctgaaaaagc gactgagtag ccaggagaga ttgcttgtgc caacggtata
      181 ggagataaaa ggctactttt atgaatgctt cccatatcaa aaccattggc gtgataatgt
      241 gaacaattaa ttttcttacc gttgtaaaga tacgtacatt cgataattga gtttggcatt
      301 tttgatgggt ttgcaattac gccagtaaga atcattcgat tacttctatc atgcttaact
      361 aaaaaatagg agagcgtaat ttttctttta tctttaaata agttatagag ggataaaaaa
      421 aatcctgaaa tagaaatgat taaagtaata gcgacttggt aatcgctcaa aatttttatc
      481 aagtaattca ccaccttttt actcaaatta tatcaaaaag gagagaagaa ataatgcaag
      541 aattagtaat tttgaaaaat aaaaaaagac acgaacgccg gcaagcatga tcgtgtcagg
      601 gaaataaact atctttctat attttaccac agaaaggaat gaaccgtaaa tgctaattgc
      661 aacggataca ctagaccgca tctttttaaa agacgaatac aaactgcgca atatagatgc
      721 gtcaggaatt ttagtatttg atctttatga caatggaaaa attggtatct atcaagcaag
      781 tgatattgaa gaaacaaacc ttgctttcga acaaattgat gattctgtgg aattggattt
      841 agatgaggca atcctagctt ttgaacaaat tgcaaaatta ttaaaggagg cacaaaagaa
      901 tggcaactct ttaccaactc agcgagtcat atatcaaagt cctggaactg gcagaacaat
      961 tggatgaaga aattcttcgc gatactctcg attcgattaa tgaagcgatc gagtataagg
     1021 cagaaaactt agcaaaaata gttaaagaag tagaagggaa agctgagtta atcgatagtg
     1081 aaattaaacg tctgcaggaa cgtaagacat cgcttttgaa caatgctaag agtatcaagc
     1141 actatttaca agaggaaatg gaaaagactg gtaaaacgaa gattaaaggt gaattattca
     1201 acattggaat tcaaaataat ccagtatcgg tcaatgtaat caatgagaac ttaattccaa
     1261 aaggattctt tacccctgtg cctcccaaat tggataaaaa gcaattgaag gaggaactga
     1321 agcacggaga tattcctggt gctgaactcg tccaaacaaa aggtttgagg ataagatgat
     1381 gaaggaggag aaataatgga acacgaaaac gtaaaagacg ctttgaaagc tgctatcgaa
     1441 atcgcggaag ctaaaggaat aaaggttgac ggcaaaccag caactgtcca tgacattcaa
     1501 aatttgacga aagaacatct ttattttatc gccgacttgc ttggtttgtc ggaattgtat
     1561 ttagacaaat aaccccacac ggaaggagtg gctagggtgg ttgtattaca agtaatagtc
     1621 tgcgtaacat tcatttggtc gtgcataccg gtttgtatat atatcatgaa gaagcttcgc
     1681 cctgagtctt acaaattata cgcaatttac agcataacag tagctcttgc atttacattg
     1741 tttgcaattt ctaggcttcc gttttaatcg tgggaatgaa tttttaatga actaaattat
     1801 ctataaagca aaaaaaggag aaacaacatt gaaaaacaaa ctagcaaaaa caacagcaat
     1861 catcggacta gcactaggta gcggcgttat cggctacgca gcaagcaatg cgtttcaaga
     1921 tttggacacg atcaaagcaa atttcaacac agtcctacaa tacggtcaaa cgaaatcgca
     1981 acgtgtgtca gaacttgaat cacagctatc caacaacact cgcacacagg agcagctgaa
     2041 agccgaaatt gagcaaatca aatcggacaa gcagaaggaa attgaagcca agcaacgaga
     2101 agtcgaacaa aagcaacagg agatcgctac aaagcaacag gaagccgata gcttgcgcca
     2161 acaactatcc acagtgcaaa acgacaagga gcagttagaa cagcgcgtga gcgagttacg
     2221 gcagtatacg gatcaaaagg taggggagtt ggggaaatga aagagttgac tatttatgac
     2281 ccaaatattt catgggctaa acacacaatt aaagtttctt ttatgatttg gggttacaaa
     2341 ggatatgtta cttacaaagt gggcggtaac actaagggtt tatcattaat agctattgat
     2401 tcagatgatc tatatgatgc taatttcgag gataacccag ttaatttccg tgaccttgat
     2461 gaagattggt tctctatgga acttactaac gataaaggcg acagtacact tgtagaagat
     2521 gaattcgaca gactaggtga ttatattgtc ggtgttgaaa ttattgcgca cgaaccagag
     2581 taggagagtg aatacaaaat gattaaaaga atcactatat cagttgaaga agatagtgga
     2641 tcaacaatgc agcataccga caatcgaaaa acttttttaa ccaatagaaa ggagaatttt
     2701 gatgcagatt aagaaggcga cggatatcga acaaaccaag ggaacatatt tgatctacgg
     2761 tgctccagga aaaggtaaaa cttcaacggc taaatatttt ccagggaaaa cactgatcct
     2821 tgatattgat cggacttcaa aagtccttag aggtgagaaa aatgtcgata tcgtatatat
     2881 cgacaacgaa gatacctgga acgactgggg caacacgttg gctgatttaa ctaccaatta
     2941 tgtaggtgtt tatgacaacg tggttgtcga taatgtcagc gaattggaac ggtgtattct
     3001 gtccagttta ggcgcagaag gaaaaaacaa cggcgttcct tcgcaaggcg attatcaata
     3061 tatgcaattt agaatggtga acagcctacg ctatatgaaa aatttagaca gcaaccttat
     3121 ctggaccgca tgggaagaaa tcgatttgtg gacggattcg gatggatctt cttacaacat
     3181 cgctttgccg cagatcaatc gaaagattcg aaacaatatt ttgggattat gtgacgttgt
     3241 tggtcgattg atggtaaagg aagacgatga acgcggattt attttacaag ccacagattc
     3301 aacctatgcc aaaaatcaat tggataatag atctggctgt aagcaaagtg agctgatttt
     3361 agatggaatt gtacgactac cagaatgatt tagtcgatcg tgcaagacaa gcctatgttg
     3421 atggttacaa ggcaccatgt atcgtatcac cgtgcggttc tggaaaatca gtcatggttg
     3481 cagagatcgc taaacgaacc acacgaaaag gcaacagagt gttgttcttg gtccatcgtc
     3541 gtgaattgat tgatcagatc aaagatacgt ttcaaaagat gggcgtagat aatcagcttg
     3601 taaatttcgg catggttcaa acagtcgtac gacatcttaa aaccatcaag aaaccacaac
     3661 tgatcatcac tgacgaaaac catcacgggt tagcagcgtc ctacagaaaa atctatgagt
     3721 attattcgga cgttccgcga ttaggtttta ccgcgacacc catccggtta aacggtagtg
     3781 ggttggggga tgtgaacgac ttgctaattg aaggagtatc ggctaaatgg ttgattgaga
     3841 atcatagatt agctccttat gaatattatg cacctaaatt gatcgataca gccgaattaa
     3901 agaaagcttc gactggagat tttacaaaga aatcaatgga taaggcagtg aagaatacta
     3961 tttatggcga cgtgttgaaa cattataaaa cgttagcaga aggtgaacag gcaatagcat
     4021 actgtcacag tatcgatgca agtaaacaca ctgctgaaat attcaacgat gctggttata
     4081 aagcaacaca tatcgatgca aaaactccaa aagatgaacg tgcagatatt atcgaagcgt
     4141 tcagaaatca tgaaattaaa atcctatgca atgttgattt gatcggagaa gggtttgatg
     4201 ttcctgattg ttcgactgta attatgttga gacctactca atcgttatca ttgtacattc
     4261 agcaatcgat gagggggatg cgttaccgac cagataaaac gtcaatcatc attgaccatg
     4321 tgggtaatgt gaaccgtttt ggattgcctg atatggatcg tgaatggagt ttagatacaa
     4381 aaaagaaaac aaaaactgat agtgatttat ctatcgttca atgcgcgttt tgttttggtg
     4441 cttacgaacg gccaaaaggc gataatattt gtccgtattg cggagagttg cagccgattg
     4501 aagaacgaaa gtcagagtta gaaatcgatg aatcggctga attaatgaaa gtcggagaaa
     4561 cgaaaattac tttgaatttt gagaacaata aatattacaa catgacggaa gatgaagcag
     4621 aaagtataga ggacttatat gccatagcaa gagctaaagg atttaaacct ggttgggctt
     4681 atatggctgc taagaggaag gggtggttgt aggaatgggc ggtatttttg gtaaaaaagc
     4741 agaagatgtt caaggttcaa taataaatgg cgtaacaata ataggtgata ctggaaaaag
     4801 agatagttca ggcaatcaaa ttgtaatcgc tagaaataaa aataacgaag tttttgaatc
     4861 ttctctatct aatataaaaa gaggtgttgt taacgggaca ggcgaagaga gtggctacgt
     4921 taatttaaaa aaagctaatg aaaaatacca tgttgaagga acttacgtta aggctttgtc
     4981 tgtcggtgga acaaaaggcg ttaattggga taaacgaaga aataaatgga aagcacgatt
     5041 atatttcaaa ggagaatatg tcttacaaaa ggatttcaac accaaagaag aagcaatcca
     5101 agcacgactt gcagcagaag aaaaatattt caaacctatc ttagaaaaat atgaaaataa
     5161 aaaggagaat gattaacatg acaggattta acttagactt ttcaaaagcg caacaaggga
     5221 acgaaatcaa agacggtacg tacgaagtgg tcgtaaacaa agctgtagaa aatgcaacta
     5281 aatcaggcgc agaatttatc gatatcgatt tgatcgttcg taacgatgtg gatcaaccgt
     5341 tccaaaataa acacattttc gcaaaaatct ggaaagccaa agctaccggc aaatataacg
     5401 aaggtatgat catggcaatt gcacaagcgc tacagttgga agacggaaaa tcatataacg
     5461 ggtttgatga attgctggcg gactttgttt tgaaaaccgc ctcagtacgc gtgaaaactg
     5521 aagagtcaaa tggatataaa aacgtcaatg tgaagtcttg ggataaaacg aacacacggg
     5581 gcgtaatgaa tcaccaattc aaaaatggtg atgaaccatc cttcggaccg gaacgttcaa
     5641 gagcgacaac ggttagaaat gacaatcttc cattttaatt gaggtgtaat caatgtatga
     5701 aaaaattcca aaagagctta aaaatctcaa acaatggtgt gtttataagc tagtttggga
     5761 tgaaaagcgg cagaagcaca cgaagatccc ttataacgcc aacaacgggc ataaagccaa
     5821 atccaatgac gagagtacct ggtctgattt tcagaccgct ctcgctgcta ttgatcacta
     5881 cggaatgagt ggactggggt tctttttcaa accaccttat ttcggcattg atatcgataa
     5941 tgctgaaggt gaagttgagc gatttaaatc aggtgatata gaagaaaata ttatctatga
     6001 atttatcgaa agcatgagat cctatgctga gtattctcaa tcaggtactg gcatccacat
     6061 tattgctcgc ggagaattgc ccggcggaag acgcaggaaa ggtgacgtag aaatgtacca
     6121 gaacggccga ttcttcgtta tgactggaaa tgccgcatca aaatatttag aaataactga
     6181 gccgaatcca aaggatatca aacgcctata tgatcgctac gttggagatg aaaaaatcat
     6241 ccaattcaaa gaagaaaatc cgctgatgaa taccgttgat ttaccggttg aagaaattat
     6301 caaacgtgct gaatcgtctt cccaaggggc aagattcaaa gtctttatga acggcggttg
     6361 ggaagctttt tattcctcgc aatccgaagc ggatatggcg ttcgccaacg acttggcttt
     6421 ttggaccggt agagactttg aaaaaatgga tgagattttc agagcgtctg caatgatccg
     6481 tagcaaatat gaccaaaaaa ggggatcggc aacttacgga gaatctctac taaataaggc
     6541 aattgctgat acaaattccg tctacaaccc caaacggaaa tctgatttta agatttttat
     6601 caaagaccag gaacagccaa aagaggaaaa atactattcg tacgacgaca ccggcaacgc
     6661 ggatcggttt actgatatct acggaacgtt agtcaaatat tcgtacattg ataaatcttg
     6721 gtattactac gatgggaaag tttggctaca ggacaatacc ggcgaagttc ggaaaatgat
     6781 cgatacgaca gtagacatca tgggaaaaga accgttaacg atccctgaag gcgcagacga
     6841 tgaaaccaaa gaagctttga tgaaagcgaa agaaaaacac gtcaagcgtt ctcgcagcaa
     6901 tgccggaaaa aacgctatga tggacgaatt gaaacatcga ctatccgtat tgccggaaga
     6961 gttcgacaaa gataaaacgc tgttcaacac acaaagcggc tatctgtctt tgcatgacgg
     7021 tcttctacac gagcatgaga tagacaaaat gtttacacgt gtttcaaatg tcgagtacac
     7081 agaatcggtc gattgtccta tgtgggaaga atttattaat cagatttttg acaatgatca
     7141 agaattaatc agatatatcc agaaatgtgt cggatattcc ctgaccggat ctaccagaga
     7201 gcaatgtatg ttcatcctct acggtcatgg gtcaaatgga aagtcggtat ttttggaaat
     7261 tatttctgaa ttgatgggaa actatgcgat gactatgcag gcacaaacga tcatggtcaa
     7321 gcaaagccaa tcatcagcaa acagtgatat tgcacgattg aaaggtgcgc ggttggtcac
     7381 ttcgtcagaa ccgaatgaag gcgtccgact ggacgaaggt ctggtcaaac aattgactgg
     7441 tggtgataaa gtcacggctc ggcaccttta tggaaaagaa ttcgagttcg aacctgaatt
     7501 caagttgtgg ctggcaacga accacaagcc catcatccgt gggaccgatg acggcatctg
     7561 gcgacggtta aatttaattc cattcactgt ccaaatcccg gatcacaaaa aggataaaaa
     7621 tctgaaattt aaattgcaga cggagttgca agggatcctt aaatgggcaa tcgatggttg
     7681 cctgttgtgg cagagagagg gcttggagaa accaaagtcg gttgttgcag cgagtcagga
     7741 ttatcgaaac gaaatggatc aaataggtac atttatcgaa acgtgttgcg aaacaggtcc
     7801 aggactaaaa atatcaggcg gagaattata caaagtttat cgtgaatggg catcagataa
     7861 tggcgagcat actttcacta acacgaagtt tggcagagag atttcaaaga aatatagcaa
     7921 agaaaaagct ggtggattta tggtttataa aggaatcaca ttaaaaccaa gaaaatacga
     7981 taacgttaga gaacttttta aataaatctg gagggttctg gagggttgag aacgaaaccc
     8041 tccaaccctt agaaacgttg atatatcagt atttatatta tatttttctt tctttctgga
     8101 gggttttaaa aaaataataa agtattaaag taaaaaataa aaagtaaatt atataaaaaa
     8161 gaataggttt ttgggtctga ccctccaaac tctccaccaa aatcttccta aatgctttga
     8221 taccaacgat taaacagtct ggagggttac attgaaatca gaacatcaaa tacaagacga
     8281 gatcagaatt gccttatcaa aacatggttg cacgattttt cgatccaatg cgggacaagt
     8341 gacaacgatc gaagggcggc aattttacgg gatgccaaaa gggttcccag atctatgtgg
     8401 acatcgtaat tctgatggga aagcaattta tatagaagta aaaaatgaac gcggtaagtt
     8461 gcgtgaagat caaaaaaggt ttgctgaatt tttaaacggt caacctgtac tatacggtgt
     8521 tgcacgatca gtggaagaag cattgaaaat tgtggaggaa tcatcatgac aacagaagaa
     8581 gtgattcaaa tgcgtattcg aagccttcag cgtgagattg acgatctgga acggacaaag
     8641 gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt tgcacataga gaatttaaga
     8701 agggaaatcc atcgattgga ggaatgagcg tggataagaa agcagcaatg aaacgaatca
     8761 tcgaactgac acattctgag aattggcaag aagacaaaga aatagttgca gaagtccaaa
     8821 agctcggtaa atcaatgtgg agtgaaaaaa ccaaacggag aacaccgaga aaaattgcaa
     8881 tctggcatgg tgaccgaatt ctagtaacag gtactgctga acagttatct gaaattactg
     8941 gattaagcaa aaacattatc tgggatagag ctaaacgcga gaacgttgat tcgaaaggtc
     9001 gtcaatttaa acattgggag aagaaataat ggaggaacta atcacaaaag tagagcagtg
     9061 ggcaaaagat aagggattgg atcaagctga ttccagcaag caaatgttaa aaacgatcga
     9121 agagattggg gaagttgccg cttctctagc tagaaaagat gaatatggtt taagagacgg
     9181 aattggagac gtagtagtaa ccttgattat tttagctatg caaaatgata tggatttgta
     9241 cgagtgtctg aaccaagcat acaacgaaat caaaggacgc acaggaaaaa tggtagatgg
     9301 tgtattcgtg aagtcgagtg atttggagga cagcgatgaa taaaaaagta ttaattgata
     9361 aacaagcatt gattgatgaa ttaatgaaaa tacctggtgt gggatctaat agtgacgctt
     9421 tagaaacgat taaacgtttc ccatcgtatg aaccgcagaa gccagttgtg cctaagtttg
     9481 tggcggaact actcgactat tatcgtcagt caacagatgt tgatttatta gctttattga
     9541 taactttcca tgattggtat tatcgcaaaa ctaaagacgg tgagcatgaa gaagcaattg
     9601 attggctagt tgatcatcct gaaatttata tgcgtgcatg gctgtttggc tacgagctcg
     9661 agaaagagcc gttgtattgc gtaataatca atggtagata tcttgtcaaa gtattcagca
     9721 atacaaacgt tgtcatatta gttccagcag atgaattttc acagtacgtt actcaaaaat
     9781 ttcaattaac tgaaaaacaa atcaaagcaa ttgatgaaag atactggccg tttgctgtgc
     9841 cagtggatga ggtggtagaa ggatgagcga cccatacatt actaaattag aaaagttgtc
     9901 cgaagatttt gagaagaata aaaataaact aactaaagat aaacttatag aactggttag
     9961 gttggttatc agaggagcta ttacaagaga aaaggcgcag caaaacatag aaatttttgc
    10021 tcgtagtgtc cgagatggat tagagaagaa agttaaacaa aatgaacaac agacaccgca
    10081 gaataacaaa actaagaaaa cgggaactga atgtactaaa gacaaagttt gaaaaagaat
    10141 atggaatttc agcagaagaa acatataaag tggcaagtca gtgtgttgct gatgcgagcg
    10201 gcgctattcg taagtttgga atttcgatat taagtgatga tcgtaaatgg gaggaaaaag
    10261 aatgaaacta aaagacggat tttactccag cagtcatggt atcggcggtt taatgctaga
    10321 tatgccgaca aagaacccta aaacacgtga gaaaccaaaa ttcaaagtcg gtgacatggt
    10381 tcgctgtgaa gcagaaggat tcatctatcc attttgtgga tatgtagaac atctctataa
    10441 tcactcagca atcattcgta ttgaaaacac gatggaatgt gacaagtggt tagcgaaaag
    10501 caaagagaat ttagcagtag cgagattggt ggatatggag gttatataaa cagcgaaaaa
    10561 atgtacttct aagagaaaaa tttatttttt ataaaaatag gaaagtatca ttatgaaaaa
    10621 gaccacagag ataaataaac ctaccacata aaaaatgaat atttcttgcg attcaatata
    10681 ggtatattta acaatttgta caataaaaaa tactataggt aaaaaagata acatgaggag
    10741 cttatttgtt ctagagtgtt tgacaaagta acaaaatgcg acgataccaa tacaaacggg
    10801 aatccaaaat aacagcatgc tccacatagt cactcaacca tctcctatac gctttattat
    10861 caatagataa tatcattttt cgtaataaag ataaaaaaat aagtaaggct tctcattaaa
    10921 atataaaaaa gacagccgac cactgactgc ctatataaga gtattgaaat aaaaagctgc
    10981 tgatataata aattccacaa gtttattata tcacataaag gagcggtttg acttgatgca
    11041 attgttacga gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa
    11101 ctttcggcgt ttggagcgga tggcaggtcg ctctttgata gatattaagt cgccgattat
    11161 tacggatatg ccgaaggcgc cgaagcacgg caataaggca gaagacgcaa tcattcagat
    11221 gatggatata gaagcggaga gagacgcgat tctagcagcc ttgatggctc ttagtctgat
    11281 tagtcgtcag atactctact atagcttttg tgtgccagat agcttctcaa actacagaat
    11341 tagccgtgaa gtgggttatt cagaaagaag tatacaacgg atgaagtcgg aagctctaat
    11401 agagtttgca gaagcatata aacacggaag aataattgct tataaataat ttggcggctt
    11461 tttggcggaa tgatggcggt ttttagctat ttaccagtga tattatggta gtgtcgaaag
    11521 attagtgata ggtctaagac aaaataataa taaaaggaac atcgttttat tattgtttca
    11581 caattaagct tcgatagaca gcaacggaaa tattaagaat aaggatgtga attttaactc
    11641 cttctaaatt gttcttatta tctatcatcc gttgctgtct attgtttttt taattattca
    11701 cacgataact aaaggtgagc gaagagaaat gattccatta ataatttcaa tttttgcgct
    11761 ttgtcttaat gtctatatga ttggatttaa aaatgggcaa aataaaagat agtagtaact
    11821 aagaaagaaa tttttatatt gtcactgtgg cggaaagggt agacgctaag catgtgtgct
    11881 aggtcaatgc ttcggcaact atgcaatgtt cgattcattg ccagtgactt tggcaaccga
    11941 ggcatcggcg gtttaaaaat ataggggtgc gcaatttcgt acgcgttttg tgcatcgtgt
    12001 aggttgctat tacatattag atcactcgtt gagtggtctt tttattttac ataaaggagg
    12061 taacaacgat gtatagacca caatacttag aacagaagta tgaagtaatc actgtgcaaa
    12121 acggtaacgg tgagatagta cgaaagtata gaagaccaat aaagagcgat acatataaac
    12181 gaaaggaaaa caatgaagtt atttcttttc gcagaaggag aaaagccaaa tgagaaacta
    12241 ctggtatgta tcgctaacta atgaatatcc tcgaaccatt gatgattgtt cagtgcgtgt
    12301 tgtgcgttct gtacaaatca aagggaagta ctctattgtc gaaatgctaa gagaagctac
    12361 accaaacgaa gtggataaat gcaagctgat atattgcggt catggctatt ggaaagacga
    12421 gtatatccaa caaaacattg agaggtggat agatagatga gttacctcga acatttgaaa
    12481 cgttgctaca tgcattctaa gaataaactt cctgacagct acactgtaga tgatgttgct
    12541 atacatgtat tgaagactga gagccatagc agtcctgatg gtagcagtaa ggagcagacg
    12601 ctcgcgtggt tcaagttctt taagtggatt aaggaggaag agtaatgcac atgcacatag
    12661 gagaaaagat atataaattg agacggctta ataacgaaac aatgaacgac ttagttaaac
    12721 aaactggagt gtcaaaatca tggttatctg atattgagtc tggaaaaaag caacgcgttg
    12781 attttaataa gatatgtgca atagccaaac attataaagt tagcttagaa tactttcggg
    12841 atgattattt ggatagtgat gaacacgagg atattattcc tttgacaggc gaaacaagag
    12901 ttaaatcagt gaaatcgctt gaacaaagcc taaagtatct gaataacaaa caagaaccta
    12961 accttctaga gatcaaacta aaagatactg actcagtacc agaggtttgg tacaagggtg
    13021 agaggttgga tgaattgcct aaaggattag tagatgtctc gtatcattgg aagactgatg
    13081 attttactaa tgatgataga ggagcgaacg acatcacgat tcaatacttt tctagcttta
    13141 atgataagta tccagataag aaaacaatcg gacacaagag agatatgtaa atgaaagaag
    13201 ttagacctag agacgaaata gacaaactat acaagaccaa acgatggcga gacctaaggc
    13261 aagtagtaat agctagggac ttcggcatgt gccaagagtg caagcgtcga gggcggaaca
    13321 caaggggcac gatcatccat cacatagtcg aggcgaggga agacctgtca ctgttctggt
    13381 ccgtagataa ccttgaatgt atctgtgtag cttgtcacaa cagagagcat ccagagagat
    13441 caggcgggaa gaagaaacca aaacctaaat cacatatcgt taaaatgtat tcaactcctg
    13501 aaagataagt ttgcagcgaa atgaaggtag cccccctact ctaaaagatt aaagagtaag
    13561 gcttgagaag aacggtgctg tccttccttc gtaaaaagac cgcttttcaa gttttttgga
    13621 gaaaaaggaa aaagccgacc aatttaagcc ggctttggac gaagctattt cttagtccat
    13681 ttgtttcctt tttgagaagt agggggtaat cggtcgcctg gatcaatttt tacttcttgt
    13741 ccgccttgga catttccacc acgaggaccc acttctttgt aggttccttt tggtttgttg
    13801 tcttcgccgg gtttatagag ttctcccata ggaatccctc cttaacaaat ttcggcacag
    13861 cactgccgat aacctaatta taaggattgt gataacgatt tcaatctatc ttttgaaaga
    13921 aggtgatatt atgccgcaac cagcgaagag tgcaaaatta caattattaa acggaaaccc
    13981 aaataagaag aataccgaag aactccgcaa gcgagcggcc gcagaagaca aattaaaaat
    14041 ggctactgac aaaatcaaac cgccgttatg gctagattcg ctaggaaagg atacctttga
    14101 gtttatcgcc gatgaattgc tgtctgtgga tttaatcagt aatccggacg tccatacaat
    14161 ggctctatac tccaattggt attcgcaata cgtttcttta gaaaaacagc ttcgaaaact
    14221 acaacgagag tacaagttga actatgcgct tgcgaaaaag gaggcagagg cgagaggtga
    14281 gccgtttaat gaacctaatg aattaattgg taatccgctc tctcggcaga tggatacagc
    14341 gtctcggaat ctccgttctt ttggcgctga tttaggacta tcaccagcag ccagagctaa
    14401 gttagctatt aagatggctg atgatggtgg tgatgacgat gacgacttct aatattttgg
    14461 atatgtctta cacagaacgt gtggactatt ggcaaagcta tcttgaggag caagcttctt
    14521 ggggtggctt tttaaaatgt ccatatccgg aattgttaac tacttggtat gcggaacgat
    14581 taatcgatgg aagcatacca gccagcaaag aaaatattca agctgctaaa cggcatatgc
    14641 gtgatttgca gcgccaagga acagatgatt ttccttggat ctttgacgaa gaaaaaggtc
    14701 accggcctat tagatatatc gaaaaaaaat gtaaaccaac tgaaggcgac tttggttcgt
    14761 ttgttttgca accttggcag catttcataa ttggatccat gtacggatgg gtacatcgtg
    14821 atacaggaga gcgtcgcttc cgcgaggctc ttatttttgt tggacgtaaa aacggtaagt
    14881 tttgccgttt aaaaatcggg caaaatcggt gaaaaccttt atttttggct ttcttttagt
    14941 aataattcga tagctttatc aagcaattta gacataggaa ccatagtttc ttccgacatt
    15001 ttttttaatc cttcgtataa atctttatca attgcgttag aaattctaat tctgtttttt
    15061 agtccgtatt tgtccatgtg tgacactcct ttttatttta ctataacatc cgattccacc
    15121 gcttgcaact accatcaatt gatggtagta tataaaagag gtgagtagat tgaaaaaata
    15181 cattatttac aaacacttaa ataaaataaa tggcaaaatt tatatcggtg tcacaaatga
    15241 tattggcaga aggtggagga gcggtgggat tgaatataaa ccaccaaaga acgaaaccca
    15301 acaccacaga agtttttgga atgcaataca aaaatacggg tgggaaaatt ttgatcattt
    15361 gattattgaa gaagacttaa cgatgaaaga agcttttgag aaagaaaagt tttatattga
    15421 actttatgac tcaactaata agaaaaaagg ttataacatc gcaaaaggcg gaaatggcgg
    15481 aataatatat aaagttcacc ccaaaggaat gctagggaaa aaacaatcta aagaattttc
    15541 ttcaaatcac agtaaatggg caaagaacca taagaataat tgtatgacaa acggtgatgt
    15601 tgtatggggt gttactcacg aacacccaaa aggcatgttg ggaaagcacc acagcaaaga
    15661 atcaattatg aaaaagaaag catatagtgg tgaaaatgct gtaacctcaa aaccaattgt
    15721 cgcaattgaa cgggatggaa ctaaaagaga atttcacagc gctaagcttt gcatggctta
    15781 ctattcgatt agtacatgtg ttttttaccg acttttaaaa gatggcgccc cttatgtgat
    15841 aaatcctaaa gcgaactaca gaaataaaga aaaaatatta gctatcgaag gaattatgtt
    15901 caaacataaa gaagataccg aggtaagtta gtagattgcg aaaggttact aactaccgta
    15961 acgcgtagga gttgaataaa tataatgctc ccaagagtgt ccgacagcag ataatttgct
    16021 gaaaatgtac gctaaactgg gccagaactg actgaccgat gaaaatgagg gtgacctcca
    16081 gagtgcgaga taaaaagctc gcagataata acaatcgaaa acatcactaa tttcaggcct
    16141 ttccacatac atggtcgctt atgatgatga acaaggcgcc aacgtttacg tattggcaaa
    16201 tgctcgtgat caagcaagct tgttgtttga taaggccgca gaaatggtca aacaatcgcc
    16261 ggcgctcttt aagaaatttg gtaagcctaa acgatcaagt attaattatg ctcccgcctt
    16321 ttctaaaatg gaaccacgcg cctcagatag ccggaaattg gatgggctaa acactcactt
    16381 tggtattttt gacgagatcc acgagtttac gaattacaat ctgatcaacg ttatcaagaa
    16441 atcaagagga accagaaaac agcctctgat agtttatatc acaactgctg gatatgtatt
    16501 agatggtccg ttgatgtctt attttgagca aggtgtggac tgtttggaac atttggaaga
    16561 tgacatcgat gaacggactt tctattatct ggcaaaactt gacagtgcgg aagaggctga
    16621 tgacccaaga ttatggatca aagccaatcc gaatatttgt ctaatgaatt ttgttggcat
    16681 gctagatgac tatgttaagg ataaaaaaga tccaaaagaa tatgctgact ggattaccaa
    16741 gcaatttaac ttgttttccg atatcgatga gctgtcattt gtcgatatgc ctaccattaa
    16801 acgaaacaat aaaaccatcg atattgaaac gctcaaaggt aagaagtgtg tcggtggttt
    16861 tgacttgtcc gaaacggaag actttaccgc agccgtttta gaatttccgc ttgaaacagg
    16921 cgaggtattc attttgcaac acacatggat cccacaagct agatttgatc gagataacaa
    16981 tcaagagcgt atcaaagcgt gggagaaggt gggagatcta acgattattc ctggtgatta
    17041 cgtcaattat gaatacgtct taaattggtt tgtagaaaat tcgaaaatct atgacattgt
    17101 aaaaatcaat tatgacaagg ccaaggcgct acgattaaat aaagaactag aaaatgcagg
    17161 atttgaaacc aacgagattc ggcaagggtt tctatcatta ggtgggccaa tgcaaaactt
    17221 caaggaaatg ctattggacg gtaaggtgat tttcaacaat tccaagcttt accgatggta
    17281 tctatccaac gtcaagctgg tgatggatcg caactcaaac tggatgccgt ctaagcagtc
    17341 caagagtaga aaaatagatg gttttgcagc aagtttgaac agccacgccg aagtgttgaa
    17401 tatgttggtt aatcctgtcg gaaccgggaa agtaacctat tactcgattt ccgatttaat
    17461 gaatatgtaa gaaaggtgtg gaggaatgag tattttagat cgtttgcgtt cttttggccg
    17521 agcgaagccg aaagcgagca aacaagagta ttttttgaat gacccgggat tgataccgta
    17581 tttagtcgga aaagatgaaa tatcagaagg gattttttcc gtaattagcc gtgtatcgaa
    17641 cgtttttgcg tctctccctc tcaaaatgat agatgtggag tttggccaac cggacgactg
    17701 tcctgcatac aacttgttga gcgaaggccc tcgatatttt acaaagtttg attttttccg
    17761 ggacgtggaa gttttgagaa actaccaagg gaatgcgtat gtgcagattt tccgaaatat
    17821 caatggagaa gtagcagata tggcgttagt aaaacctggt gcttgccatc cagtgattga
    17881 tatggatagc ggggagcttt actaccaagt aactgcgact gacaaaggca gttacaagca
    17941 agttatctat gtacattaca tggaaatgct tcactttaaa caaccgaggt ttggcggctt
    18001 ggaaggtaca gaccccacaa aagtattaac gaataccctc ggatatgatc gagaagtccg
    18061 aaaaatctct ttaagtcagc ttaaaggaag taatgaaggg ctaaaagtta agtttgctag
    18121 caatatggat gaagaagcta aaaaagctac agttaaaaac attgctgatt tttatcgaca
    18181 aaacggtgga ctacttgtgg aagaaaacgg tgtagaaatc gaacgtttac aacgagagct
    18241 ggtagacagc aagcttttag atactgataa agtatctcgc tccagaatcg ctatggtcta
    18301 caatgtgccg gagcatttca tcgggaataa ccagtcgagt tactcctctc aggaacagct
    18361 caatatggaa tttttgacgt acaatctagt accgaccgtt aatcaatatg aagcggaact
    18421 aaataagaaa acactatcga gaaccgaaaa agcaaaaggt tatcgatata agtttaatgt
    18481 cgcaagtttg ctaagagctg acactcaggc aaggggacaa ttctatcaaa tcatgcgacg
    18541 aggcggagca tactcagcga atgatgtccg aagatttgag gacttgcaac cgataaataa
    18601 aaccggtatg gatgattacc atatttccgg agacctatat ccaatcgata tggatccaac
    18661 attaagaaaa acaacctcgt ctaaaagcgt agccgaaaac ggttaggttt ttttagtttg
    18721 caccgaaggg aggtggaaaa atgaaaaaag tgacgttaag cggcgatgtc gtggataacg
    18781 ataccgcgtg gctttatgac tggtttggga tcaattgtat ctcaccaggg aaaatttctg
    18841 ccgctcttac agaagcagcg ggggatgaag tagaacttga tatctcatcg aacggtgggg
    18901 atgtcctagc ggcaagcgaa atatataccg ctatccgtgc ctatccggga aaggtatccg
    18961 gaaatgttgt gagtattgca gcaagcgctg caagtgtaat cgcttgcgct tgcgaaccgc
    19021 ttagaatctc acctacggca cacatcatga ttcataacgc atgggtgacc actagtggca
    19081 acgctgagga attaaaagcc aatgcagaaa tgttaagcag tgtggatgag tctattgtta
    19141 atgcttacga gatcaaaaca ggactcgatc ggaaaaaact tgctgattta atggcgaaag
    19201 atacttggtt aaatgctcaa acagcagtag cagaagggtt tgcagatgaa attatgtttg
    19261 cagaagcacc agtaacggta ctcaatgcct ctcaaccggt tattccaaaa aacgcagtaa
    19321 ctaagttgaa aaatttaata ctcaaagcgg aagcaccgca agaagaaaca ctcttacaga
    19381 aaaaactaaa agccttaaat ggagggaaaa acgaatgaat ttagaacaat taaaaaatgc
    19441 gtgggtcgag gcgggaagta aagtctctga cttaaatgca caactcaatg cagcattggt
    19501 tgacgatgaa aaaacagaag aagatgtagt aagtttgcaa gcacaagtaa aagcagcacg
    19561 ggctaaacgg gacggattga aagagcaagt ggcaaatatg gaagccgaac aagtcttaaa
    19621 cgtcaaaaaa gaaccattag ataaaaaaga tgaaaacttg aaaaacaagt ttatcaaaga
    19681 ctttaaagcg atggtcaatg gtgatcctgc tattatggct actttgacat ctaatacgga
    19741 tgaatctggt aatgctatcg gattgactat tcctgtagat gtgcaaacga ctattcatac
    19801 tttggttcgt cggtttgact ctttgcaaga atacgtaaac gttgaaaaag tgaccactac
    19861 cagcggttct cgcgtttatg aaaaatggtc tgatattaca ccgttgactg ctttggatac
    19921 tgaagacggt gaaatcccag caaatgatga tcctgcactt tacttgatca aatacttgat
    19981 caaacgctat gcaggtattt ccacagtaac taacagcttg ctaaaagata ctgccgaaaa
    20041 cattttggca tggttgtcta aatggatcgc gaaaaaagta gttgttactc gcaatacaaa
    20101 aatcttggca gctattgatg gaatcaaagc ggcacaaaag aaagatgtta cagatgttga
    20161 tggaattaaa gatatcgtaa acgtccaact tgatccagct atcgaagcta catctatgtt
    20221 tattacaaac caagatggct tcaatgtttt agataaagtg aaacgtgctg atggatctta
    20281 cttgttacaa aaagacgtag cttctgcaac tggatatacc ttcttaggta aaccaatcaa
    20341 gaaaattgct tctcgtttct tgccaaacaa aggtacgcaa gctactccta aatatccact
    20401 gtacattggt gatctgaaag aagccgttac attgtatgat cgcgaaaaca tgagcttgct
    20461 gacaacgaat attggtggtg gagcttttga aacagacacc actaaagtac gcgtcatcga
    20521 tcgcttcgat gtgcaactag ttgatgatga agcggttgtt ttggctactt ttacaactat
    20581 tgcgaacgag acaccggcga aagtttaagg agctgattcc ttatgattct tgatcctaaa
    20641 atggatttag gcgaaatcaa aaacgcacta aagattgata ccgatgatga cgatgtggaa
    20701 gtaagccgtg cggcacaagc tgcaattgca tacattagag gggctatcgg gaatgataag
    20761 ccctcttttt ataagcaaga aaatgaaacg gttgatctga ttaatttagc tatcctgcaa
    20821 ttagcagatc attattacaa cgctcgatca gcaaccgtga gtgggaactt gcgagagtac
    20881 gatttaggtt ttacaagcct aatcttgcaa ctcaaagcaa gttatttgct ttttgtggag
    20941 gaggagtagc gtatgcccct tatccaaaca ggaaatttaa atcaacgcat caagtttgtc
    21001 cgagatacga cggttaagga tgaggacggg caagttgtcc cgacttctac aaccattctt
    21061 acttgctggg caagtgtgca gacacaacgc ctgaacgata ttaagacgtc gattggtacg
    21121 gctttggaag gaacactgac gttcattatc cgctaccaac aaaaatcaga gctgaccaat
    21181 gatatgaaag tgcgttggaa tggaaaaacg tttgaaatca ttacgattac gaaaggcgag
    21241 tttgcgaagg acttcacgac aatcattgca aaagaggttt caaaatgagt gtagaagtcg
    21301 atgcaaccga agtgtacaaa gcgcttaggg aagtaaaagc aaacgttcaa cgagtggaaa
    21361 gcccagcact tagaaaagct ggggagtacg ctcaagaaga gttacgacaa aacacacctt
    21421 actgggatgg aacgaagtca aacggtaaac gtggttcgta tatgcaagaa catgctaaga
    21481 accatgtggt tacaagctcg ataaaaaacg gattggtaga agtcggctat gacaaagatg
    21541 tttcttggcg gatgcacttt atcgagttcg gaacaatcaa acaacgtcca aaaggtttcg
    21601 tacaaaaaac acaaaagcaa atcgaaaaac aagtaacaca aatcattgct gacgaagtaa
    21661 aaaggaggct aggactttga aaacggcagt atcacaagtc tattcaattc tgaatagcaa
    21721 tgaaaaaaca aagaacattg atttttacac caatagtgtt ccggaatcag ctcaaacagt
    21781 acctagcctt ccagttggca gaattacaga gatatccggc aactatgaag attttgcaag
    21841 caacaatcct ttgaccattc aatttaacgt acaggtagat gtatgggtgt caaccatgaa
    21901 agaggttgat gccttttatt ttgcccttga tgaggttatg agggggaatg gttggcaatg
    21961 cgcatacacg gaacaaacag atgacgagga cttggaaggt gcaaagcgga ttatcaaacg
    22021 atatgtagca aatatttcac taaattaaaa ggagagaaaa tagatggcaa cagtaggatt
    22081 cgagagcgtc atttttggcg taaaaacagg tgcaggcggc actctaaaag aattagtagc
    22141 agataagtcg aaaggcggag cgatcgaagc taaaattact ggattaggcg caacttctaa
    22201 cacaacatac gcttcaaacg taccgttctt cattgcaagt aaaggggttt cgtcgccaaa
    22261 agttacgctt gacgtggcag acttaatgga taacggcatt tacagcgaaa tcattggtgc
    22321 taaaaccgtg gatggtgcaa atgtaattgg ttcagaaact gaagcgcctt acgtgtcggt
    22381 agtcatggtt acagcgaaca aagaaggaaa acgcttgttc atgggattga caaaaggaaa
    22441 attcagtcat ccagatatcg acatgaaaac agctgaagac aaaggggtag aattgcaaac
    22501 cgattccatc gaaggggaat tcatttctga tgaacgtggc tatgtatact taacagccgt
    22561 agaatcagaa ggaatgacct tacaaaaatt caaggacttg gtaaataaca aagaggggga
    22621 gtagttaacc ctgcatctac accaacgaca gatacaggga caccaaaaga accagaacca
    22681 aaaattgata cacaaggtta gccatttttg gctagcctta ttttttgtaa aaacaaggag
    22741 gaaaacaaat gattgaattg caattgaaac ttgacggaaa gaaaaaaaca ttcaaacaac
    22801 aagatatttc cgcacgtgca atgcgtgagt gtatcaaatt ttacgagaaa gcggaaaaag
    22861 cagacctaac tgatttagaa gcgattgatt caatgattgc aattacagca gatattttcc
    22921 aagatccagc agttacattt gatgctattt tagacggttt gactgcgagc gagttagtac
    22981 cggcattaga aagtgttttt gaacaaataa atgaactggg aaacaatgaa aaaaagcaga
    23041 cggcgagcaa aaagaaataa gtttttctga agctaggaaa gcaatggatc aaatctacaa
    23101 agatttaatc gaagcaggtt ggacgatgag agatgtggac gaagccgact atcattattt
    23161 gttacacctt tttggagaag tggagagtgg cgaagaatat gtagatggtg ctgatttcat
    23221 caaacaattt ttatcggctg aagacttagt aaaacttgag gaaggaggta aataatggca
    23281 ggaaaaggac aaccggcagg aaatatcaag ctagggatta gtttagatag cactagtttt
    23341 ggtaacacgc tggacgaaat caatgcgaaa gtcaaacaag ctgagtcgaa tatgcgtgcc
    23401 aatctaaagg cttatgattc agcaggacgt tcatacgaag cacttagtca aaagacgaaa
    23461 gacttgtcta cggttatgga agggcaaaac gccaaagtaa gagaattaac aaagcgccgt
    23521 gatgaagcga ttagcaagta tggcgaggaa tcgaaacaag ttgctaacct taacacacag
    23581 ataaacaatg ctaccgcaaa atataatgct tacagtcgcc agttgaacga cacaaaaaaa
    23641 gaattggtgt attccaaaac agccgtcaat gatttatcta atgaaatcaa agaaaatgaa
    23701 cgacaaatga acgccgaagt aaaagcgttg aaagccgctg gtgatgaatc tggtgctttt
    23761 gaagcaaaac aaaaagggct agccaaacaa acggaattat ccgagaaagc tatcgaagaa
    23821 cagcgcaaag ttgtgaaact gatggctgat gagtttggcg attcagcaaa tgaaaccgaa
    23881 gatgcaaaaa gggcattaga aaagttagaa cgacaaagcc aaatatctag caggcaatta
    23941 gaagcactca aaagctccag cgatcaatca ggaaaagaaa tagaagattt tggcgacaag
    24001 tccacaaggt cagctaggaa actggacgga ctaaaagaca aattaagctc gctaaaaagc
    24061 gcattttcgt ttggtgcagt tgctggatta gcgcataacg ctattagcag tgtagtaagt
    24121 ggcgtgcaag gtttggttgg cgaagcagta aacgcatcgg attcattgat gaagttttcc
    24181 aaaaccatgg agtttgctaa ctttgggaag tcacagatag aaagctcgaa aaaagaaatg
    24241 aaagactacg ccgataagac ggtttatggt ttagaagaaa ttctgaacac aaccgcacaa
    24301 ttggcatcta atgggattcc taactataca gaactaacca aggcggcagg taacttgaat
    24361 gccgttgcag gcggttctag tgatacattc aaatccgttg ccatgatgct aacgcagacg
    24421 gcaggagctg ggaaactaac aactgaaaac tggaatcaat tagcagatgc gataccgggt
    24481 gcttcaggat tgttacaaga cgctatgttg aaaaacggag cttatacagg aaacttccgt
    24541 gatgcaatgg cgcaaggtca aatcacttcc gacgagttca accaagcaat tgtacagtta
    24601 ggtatgaatg acggagcagt taaggcagcc acttctacag acacattgag cggttcttgg
    24661 gagcagatga aatccactgt aataaatggg ctacaaagta ttatagaaaa aataggcgtt
    24721 gaaaatatca ctggtttcat caacagagta acaaaaggga ttgaaaattc tattcctaaa
    24781 attactcaat ttataggttg gttgagagat attggaacgt ggatcgttga aaatagagag
    24841 ccactaacat ggattgtcgg aatcataggc ggaattacat tagcagtaaa agcattgaac
    24901 gtagcaagta tgttgctggc aattactggc ggaacattgg cagccccttt tgtggcgatt
    24961 ggtgtagcat taggcgcact agcaggtgct ttggtgatag cttatacaaa atctgaaaca
    25021 tttagaaata tagtcaatgc ggcttttaca gctgtgaaaa acgtagttat gagcgttatc
    25081 aataatttgg tggaatacta caaaatgttg tggagcgtgt tgcagtggct ttgggaaaaa
    25141 ataaaagaat gggcttcatg gattggcaat aaattcattg aaatgaagaa cagcgttgtg
    25201 aatacggttc agaacctatg gaatggcgtg aaaaacttct tcagcaatgg cgttggagat
    25261 acgtggaata aggtagtcgg ttgggtaaaa aacattttca acaaagcaac tgaattgaag
    25321 aacaacgttt ctgatgtaat cggtaacctg tggaatggta tcaaagacac attccgtaga
    25381 ggtatcgata cggtattcaa ttggttttca gaactaccaa cgaagatgaa gaatgccatt
    25441 attggcggta aaaacgccat tgttgatgcg ttcaaaagta ttttcaacgc agcacttaaa
    25501 gcgataggta aaccagttaa cgcaatcatc catggagctt catgggtact agaaaaattg
    25561 ggcgcagaac ctctacaaga atgggatgta ccacaatacg ctacaggtac accagcaggt
    25621 gggcacccaa tcaatggtcc aatgatggtt aatgatggac gtggagcaga aacagttatc
    25681 acaccagatg gtagagcctt cattcctaaa ggacgcaatg ttgttctaaa cgcaccaaaa
    25741 ggaacgcatg tcttgacagc agaagagacc gcgcaacttc aaggttctaa agctcctaag
    25801 tatcgttaca aaaaaggtac taacttcttt ggtaacatgt gggatagtgt gaaaaaggtt
    25861 gctggtaatg taggtcacac aattaaaaac gtagtaggtg acgtgtggga ctttatttca
    25921 gacccgggag cgttagctag aaaagtactt ggggggttag atgtattagg tggattgaca
    25981 aagtatccat tagaagtagg taaaggcatc ctatctaaag caacaagtgc actgactgaa
    26041 aaaatcactg ggttgttctc atctggtaac ttagatacct ccgtaggaac aaatggcgtc
    26101 tataaatatt tggctgatgt tgctaagtct gtgatgaaga aattcccagg atttgtggca
    26161 actagtgggt atagaccagg tgacccttat tcacatggta aacgtaatgc cattgatatt
    26221 gcattaccag gcgtcacagg aggctcacct agatacacag aagcagcaaa ctacgctttt
    26281 gacaaattcg cttccaaaat tggttacgta atcactaatg ggaaagttcg tgaccgttca
    26341 ggacaatcag gtcaaccagc aactggtgca tgggagccat ggcccgatgg agatcactat
    26401 gatcatgtgc atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc aggagatagc
    26461 gtgggaggca gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt gaaaatgacc
    26521 ggtcaataca gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac agagtcaaat
    26581 ggtaatccta atgcagttaa caattgggat attaacgcca aaaatggaac accatcaaaa
    26641 gggttgctcc aagtgattga cccaacattc agacagtatg caatgccagg attcaacagc
    26701 aatatttttg acccactatc taacatctta gcttcaatca gatacgcact atcaagatat
    26761 ggctcactaa caaatgccta tcgtggagtt ggttacgcaa acggtggaat tgtaaaccaa
    26821 catcaaattg cggaaatcgc agaaggaaac aagccagaaa ttattattcc gttagataag
    26881 gctaaacgat caagagcgat gcaattattg tctatcgcaa tggacaaact aggagtttct
    26941 cctaaaactt atggaaatgc gacaactgtt tccagtgatt acgaaatcct agaagccatt
    27001 gaaaaacagg cggcaacaac gaatcagctg ttatcgttat tgctggcatt tttcaaagga
    27061 aatagccgaa ctgatagaga tttagcttta gatattcaga agatcttggt taggaggatg
    27121 tagatgcgaa ctgtattact gagaaataga ataaacgaag aaattgattt gtccacagaa
    27181 gactattttg ctactgggtt gagtaatatg gggtttgaag taaaaaaaga acatgtggga
    27241 cagtggggga atttcagaga aagcagtgag agcgttgaaa tatctgaatt tcagtcatct
    27301 gtaatcattt ctgtgcatgg gtttcgagaa aaagaactgt acaattcact tgtgcagttc
    27361 ctatcggaag gtccgtttga actggaattt gcttttgacg gcgaaacaat ggtaagaaga
    27421 tgcagtctaa aatctttaag taagactgaa atcgatccga aaacatcgtt actaacagac
    27481 actttagaac tatatttcac atcaaactgg tattccgtaa aacgcgaaaa attaattcag
    27541 cgaccgaatg tagtgaaaac acgtggtaag gtttttccgt ataaaagatc ttatatctac
    27601 actcaaaact tgtgggagaa aaaaggtgta ttcaaattca ataataattc tgtataccta
    27661 acgaatagca aggaacggat gtccccgcta aaaattcgtg tgattgggaa gtgttcaaat
    27721 ccgtattggg aagtaatcca aaactcacaa atcatcgcaa cggatggata cttcatagat
    27781 atgactgaaa cacagactct agaagtatca agcctttttg aagatacaac ggcgatttta
    27841 aaagatattg caggggtaga atcttctgtc taccaacaac aggattatac aaaaactaat
    27901 tttgttcagg ccccaactgg agaattcagc attgtgtttc atgttggggg ggcagacgta
    27961 gagattgagt tatacgagga gcgtgatctg ttttgatttt agcagtcacg ctttttcatc
    28021 gtgatttaat gctttataac gaacatttat tctctaacag ttttgaattt ggtgtggacg
    28081 agatcaacga agaggctagt agctttacga tggataagta cgttcctgta aaaacaggcg
    28141 attttctact agcaaaatat attcctagtg gaaaatttgc atattttggt gtaattacgt
    28201 cgcaggaaga cgaaaaaatc agttgtaaaa gcttacttag tttagctgat agtgagatac
    28261 cgactgcacg tgtgtcagga gataactacg aagagcatat ccggcggttg attgaatact
    28321 atttgctgaa tgatccaacg aaacaactaa aagatatttt agacgtcaaa gcagaaagtg
    28381 cgacctctca ttcgtatcaa gctaccgata cgaataaaca caagttaagt gcgtatattc
    28441 tcaatggttt caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa aacagaaaaa
    28501 tttatacagg catacgtgct gtaaatgaat caatttacat taaagacaat tcttctgaat
    28561 ttagcgattg ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac aagctattaa
    28621 tcgttgataa agcaatgaaa gatatagaga aaccgataat actgtcaaca tggtatttgg
    28681 acgaagaaaa caatttgaca caagatggat cgaaagaaaa tatcacgaaa ccaactgtca
    28741 atttggttaa tatctacgat caaaccgcag aagataaagc atcctacgaa gatgtggcaa
    28801 aatcagagtt gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt gtaagaaatg
    28861 caaaaaattt gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt tcttataaag
    28921 gaaagatata caagtcggtt ttgacagctt ggcgaatatc aagtgataag gagtttgtgg
    28981 aattgacttt cggaaacata agaagtcgtt ttatggatta ttttgaagat aatgggggat
    29041 aaaaaatggt tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta agcgcagaaa
    29101 atgatgctag actttatcac gttttatata atcggaaaaa tcaggttatt gatggttacg
    29161 atcagtctat gaatttatct tcaagcggat taacagtaaa agttgctgca ggagcagcga
    29221 ttattcaagg tcgtatggtc gttgttcgac aagaagaaag tataacagtt ccagcaaact
    29281 caagtggtta tatagcatta acagtggatt tgacacaaga agttatacct ggatctattc
    29341 ttccagaatc ggaagaatat gaatggacta ataatcaagt caagctagaa tttataacaa
    29401 aagttataaa aggtaattta aacaatggtg ataaggtcta taatttacca ctatgctcag
    29461 ttaattctac tggatcaact gtttcaatct caaagatatc ggatagttac gagctgactc
    29521 tctctaaagg agaaattttg tggaggggga ctgcgttaat gcatgatact caaactgtcc
    29581 aaccttcaaa aaaaatttgg cagacagttt caggtttttt gttagtatgg ctcccatacg
    29641 aaaacgggca agcaattgag gatagatatg taactacgcc tttttataag gagcgtgtaa
    29701 cttttactaa tgttttagga gaaattgttt caggatttga tgactatcac aaaaagtggt
    29761 ttagtaaacg aataaactat aattcgaata ctaatatatt tacaggtgca gcaagcaatg
    29821 caagtggaga taacgcaaat atggtgctta ggtatatagt ttctttttag ttaggaggta
    29881 tggaagagtg gcaaatttag aaattaaatt atctgcaaat aagagacagc cttatctacg
    29941 tcaccgtgtt gttggtagag ttggtgatgg ggggctcaca acaatcaatg tacaacttct
    30001 tgaggaagat gaaattacac cttttgtaat taatctaaac ggtactttga aatttgtggg
    30061 cgaagtttca aacggtaact ataccgaggg agaaccagaa ataatcgatt cgactaatgg
    30121 gttaattagt tacacgttca ctaagtcaaa tttcagcacg agtcatcaat tcaaacaagc
    30181 atattttgaa tatgtagatc ctaacggcaa aaaagtaact tttcagaact tcatcataga
    30241 cgtgttagaa cgagtggata ttaattcgga gcaagcgaaa tactatattt cttcattgga
    30301 aaaattacag agtgaaatgc aaaccacttt caatcagttc attagtgata aacaggtcca
    30361 atacgatcaa atctactcga aatataacga attagtaaga ttgataaatg aatcagataa
    30421 gcaagtaaat gatcgtattg atcaaaccaa tcagcaaatc ggcgatctcg gcaagctgaa
    30481 aaagatgtac agtaacagca tcgactttgg gggctatgat tattcgggga atccgaattt
    30541 gttaagtaag ctatcatacg acttaattga aaatcaaaat acttcagctg gaacactttc
    30601 taagggtgaa aactcgttta aatataataa gatatcagct gaagtggaag gtggagtaga
    30661 gttatattat aaacgaagag gtatagctaa ctggttaccc tctaataaaa cgcttgtaat
    30721 gactgttaag cttagagctg gagcggacta tagtccagtt gacggaaaac ttatactgat
    30781 tagatatagg tatgttgaca gtggaactgg caagattgtt ttagacttac ctattaacag
    30841 taattcgata actcaggaat ggaaagagtt tagtattact ggaactactc caacatttag
    30901 cccacaagca taccatcctt ggatacaatt tagggctcaa gatggggtac ttggggaaat
    30961 agaaatgagc tatgacatca aaatcgaaga aggctctaca gctacaccat atcagcctaa
    31021 cttacttgat gactcttact ggctaggtaa aacaccgttg ggtgagaatt tgataacgaa
    31081 tgccaagttc ccaattataa cacgaagcaa tcctatttct ggttttgata tttcagaaga
    31141 attaataata ggtgaaactt atacggtatc tttaaaaggg actaagccag ctactaaaga
    31201 attccactta tactacggag aagcaaatta ccagcagttg cagtatcagg caactttact
    31261 tcctgtggaa ggattaacag atgtgtggag tgccactttt acagccagaa atactagtga
    31321 aacgacaaat ttacttagag tagcgttatg gcaaaaacct aattctgcaa catatgatac
    31381 tgttcaaatt gactggctca agattgaaaa aggcgacaca cgcacaccaa atatcagtca
    31441 gtttaaatac tttggtgaag gcttgaaaga cagtaacaat ccgaacgact acagctggga
    31501 tgtcacacca gagtatactg aaaaaagctt gaataatacg gttagtctga ctgaaccgca
    31561 atccatcgaa ggattgaaaa atttcgaaga tggtattcaa tcaaaaggaa aatctgtatt
    31621 gacatcagac gacaacaaat atgaagtcgt aaccttaaca gttacaaacg ggaataccgg
    31681 atcagcaaag ctttatcgtg aaggaaaaac cgtcactatt tatttttttg cgttgaatgg
    31741 gaaaagcagt ggtggaaatg attcaacgat actaacaatt ccagaaggct atcggccacc
    31801 aattagtttt gagcaactgg ttggctcgat agaccgttct actttgaaca gtgctcagtt
    31861 atctattggt gcagatggag ccattaaatg gcgaagaaac tcaagttatg gatcggatta
    31921 tacctttgca attacttaca cgatttagaa aggcgtgaat cgatatgaag gcagcatata
    31981 gaccaattga accttacgga ttcgagcaaa tcattgtgaa tgatgaagaa catttaccgg
    32041 aagaatgcac agaagtcgaa ccaccgattc caaattggaa accgaaattc aattactggg
    32101 agggaaataa atgaaaaaca tttggaaata tggccgtact ggcggagagt atgcaggaaa
    32161 agtattggac gacatgcttg tatccgttcc ttacacagat cagccaccgc tcgaaggggt
    32221 tcgttcagat ggcgaaccgc taacgattgc tgatcagatg tttgatccta aattgaacca
    32281 atggattatt ttagcgaacg cgttagatca caacgattta aacaatctca aagcgatgta
    32341 cgaggctctt gaacatgaaa acggcaacct aaaacagcta aatgccaaac tcatgctaag
    32401 cgatgtagcg attaaacagg aaaatactgc attgaaagaa aaagctgaca gtttagcaca
    32461 aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc aaagacattt cagaaattaa
    32521 agagcaacta aatccagctt caaagggagg tgagtagtat gtttagtttt agcgatgtga
    32581 aaatgatgta tgattggggc tgttttactg acgatcaagt tcgtctattc gttccactat
    32641 gtatcacaga tgaagaagca gatagaatta ttagcaaaga agagagcgca tcttaagtga
    32701 tgcgtttttt tgttggaaag ttggtggaac atgaaagaag aagcgctcca agacgttgtg
    32761 gagagattag taagaattga aacaaaatta gacaactacg aatcacttag agaaaaagct
    32821 gatagtgcaa aagatttggc agacaaagcc tactcagtag cgctaaacaa cgcagaagat
    32881 atcaaagaaa tgaaagccaa taataaatgg tcgtggggtt acatgatcgg tttaggcatt
    32941 acgatcattg gctatttctt gactaaattg taaaggagtt aagaagaaat gattttaccc
    33001 gataagtatt atcaagtcat taaatggacg gttttaacag tattaccagc tgcatctgtt
    33061 ttagtagcca cgttaggaaa agcgtatgga tggaatggaa cagatatgac agtactcact
    33121 atcaatgcag tagcaacatt tttaggcgtt atcactggtg tgtctgcata taacttaaaa
    33181 aaataggagg aaaaaaatga agaagaaaat cattttatca ttgagcctaa taatggctct
    33241 ttttttattg ccaattaacg ggttcgccta tacgatcaac aatgaattta atttaggtgt
    33301 aaatgaaggt agctcacaag tagcaaataa ccaatacatc ctattgcatg aaacagcaaa
    33361 cgaaactgcg acaggacgaa atgaagcgca gtacatgaaa cgttcttggt acaatgccta
    33421 tacagcgtat attgttggcg atggtgggat cgtttaccaa gttggacaac ctggttatgt
    33481 acaatatggc gctggttcat atgctaatgc aaatagtcct gtgcaaatcg aactgcaaca
    33541 tacgcacgat aaagtaacgt ttgaaaagaa ctacaaggca tatgttgaat tagcgagaga
    33601 ttccgctaat aaatacggta ttccacttac attagacaca ccgtataatc aaccaggaat
    33661 caaatcacat ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata
    33721 cttatcagaa atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac
    33781 agacgaaaac ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc
    33841 agcaaatcca actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat
    33901 cgaaaatgcg aacttacacg tcgctggatg gcacatcgct aactataaat acgagtatat
    33961 tttcattatg gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta
    34021 tagaccggat gtaaatcaag cttataatac ttctggaaac gttggctatc atgtatcttt
    34081 taacatgcgc aatttcccta ataagaaagt atacgtcatg atgcgggcaa cgaatgatcc
    34141 agaaggaaac actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat
    34201 tcctaaacga taaaaatagc tcctcgttga ggagcagtac atagaattga aaattaacgt
    34261 taaatcaaaa aaatatttac aagatgttta tttaagtaga taataaaaga aaaaagcagg
    34321 tgaaattgtg acagcagaaa ttggtattat gaataaaagt ggaatcgtgt tagcttcaga
    34381 ttcagcatct acgattggag atagcaaagt atataatact gcaaaaaaat tatttacttt
    34441 ggattccatg cattctgtag gtatcatgat atatggtaat gctgaattta atggtattcc
    34501 ttgggaaata atcattactc aatataagaa aagcattggt agttctgttt ttaatacttt
    34561 ggaagaatac gcggataatt ttatagaatt tgtcaaaaca gcatctttta ttagaagtga
    34621 acagaccgag caagaacaaa tgattggggt ttttcaaaaa ataatttcag gtttatttga
    34681 gagtatagaa gtagatatta actttttaat aagccaaggt acacaaatag acaaagatgt
    34741 cctagtgaag ttattacaaa caaaaatgaa taccaatttg tctcaacagt cccagacatt
    34801 cattttagat attgaaaagg cgttatttct tagtaactat ggagaaatat taaaagatat
    34861 tcttaatagt atttctacaa tggaaggtgt ttcagaagct atatcagagg aaattcaaag
    34921 ttacgtttat gaaattataa ttagagacga tgtttattct tcccctacag gaatagttat
    34981 agcaggatat ggaagaatgg atattttccc taaactatat tcttataata tgtttggatt
    35041 tgttatgaat attctgaaat actcagaata tgaaagtgct caaataggaa atgacaatgg
    35101 ttcattaaga tctactatat taccatttgc tcagtctgat gtggtaaata ctgtagttca
    35161 aggggtagat ccacaaatta ctaattatct gtcgagtcaa gtcgatagtt ttgacgataa
    35221 cggaaaaaat acctatatta atattataaa gaatatttca gagtttcaac aaaatcaatt
    35281 catatttccg ttactaaaca tgattgcttt actcccagtt gaagaaacag caattatcgc
    35341 agagacttta ttaaatttaa ccagttttaa acgaaaatat actacttctg tagaaacagt
    35401 aggtggccca attgatgttt tagctattac acctaatgat ggccctattt ggattaaaag
    35461 aaaacattat tttgatattg ataataatat tggatacaga ttgcgaaagg agagagcgaa
    35521 tgattacaat aattaaacct caggaaaaat cccctgtttt gaaatttaat gagagcaatc
    35581 tagcccataa taaaatgagt ataactgata cagtatttga tgtaatggtt gagcaacaaa
    35641 aaaaaattaa taataacaat agacaaagaa aagaaattaa ccaatattaa tgttttttct
    35701 gttgcgtttt ttgcagtcat aatttaaaac ctcgctcaat cttgggcgag gtcttttttt
    35761 atttttctga aaagttaaag taaataatag cataaaataa tatattttac aaagaataag
    35821 tacaatctag ttttttgcta ttaaatgtgt aataataaat atgccatcac aacatgaaga
    35881 atgaaatcca ttattatcta gtctagtcca ttctttttgt ttgcagtatt tgtgatggct
    35941 ttc
//
LOCUS       DAZKOU010000019        34963 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000019 DAZKOU010000000
VERSION     DAZKOU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.019"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..1863
                     /gene="efbA"
                     /locus_tag="ACG827_000910"
     CDS             157..1863
                     /gene="efbA"
                     /locus_tag="ACG827_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0483070.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2021..2923)
                     /locus_tag="ACG827_000911"
     CDS             complement(2021..2923)
                     /locus_tag="ACG827_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0483071.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2920..3612)
                     /locus_tag="ACG827_000912"
     CDS             complement(2920..3612)
                     /locus_tag="ACG827_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0483072.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3655..4296)
                     /locus_tag="ACG827_000913"
     CDS             complement(3655..4296)
                     /locus_tag="ACG827_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0483073.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4334..4648)
                     /locus_tag="ACG827_000914"
     CDS             complement(4334..4648)
                     /locus_tag="ACG827_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483074.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4636..5265)
                     /gene="pyrE"
                     /locus_tag="ACG827_000915"
     CDS             complement(4636..5265)
                     /gene="pyrE"
                     /locus_tag="ACG827_000915"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0483075.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5272..5988)
                     /gene="pyrF"
                     /locus_tag="ACG827_000916"
     CDS             complement(5272..5988)
                     /gene="pyrF"
                     /locus_tag="ACG827_000916"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0483076.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5985..6917)
                     /locus_tag="ACG827_000917"
     CDS             complement(5985..6917)
                     /locus_tag="ACG827_000917"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0483077.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6914..7690)
                     /locus_tag="ACG827_000918"
     CDS             complement(6914..7690)
                     /locus_tag="ACG827_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0483078.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7744..10929)
                     /gene="carB"
                     /locus_tag="ACG827_000919"
     CDS             complement(7744..10929)
                     /gene="carB"
                     /locus_tag="ACG827_000919"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0483079.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10934..12010)
                     /locus_tag="ACG827_000920"
     CDS             complement(10934..12010)
                     /locus_tag="ACG827_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0483080.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12015..13295)
                     /locus_tag="ACG827_000921"
     CDS             complement(12015..13295)
                     /locus_tag="ACG827_000921"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0483081.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13314..14240)
                     /locus_tag="ACG827_000922"
     CDS             complement(13314..14240)
                     /locus_tag="ACG827_000922"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0483082.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14253..15536)
                     /locus_tag="ACG827_000923"
     CDS             complement(14253..15536)
                     /locus_tag="ACG827_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0483083.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="ACG827_000924"
     CDS             complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="ACG827_000924"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0483084.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16324..17235)
                     /locus_tag="ACG827_000925"
     CDS             complement(16324..17235)
                     /locus_tag="ACG827_000925"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0483085.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17232..17696)
                     /gene="lspA"
                     /locus_tag="ACG827_000926"
     CDS             complement(17232..17696)
                     /gene="lspA"
                     /locus_tag="ACG827_000926"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0483086.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17701..18399)
                     /locus_tag="ACG827_000927"
     CDS             complement(17701..18399)
                     /locus_tag="ACG827_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0483087.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18386..18772)
                     /locus_tag="ACG827_000928"
     CDS             complement(18386..18772)
                     /locus_tag="ACG827_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483088.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18919..20589)
                     /locus_tag="ACG827_000929"
     CDS             complement(18919..20589)
                     /locus_tag="ACG827_000929"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0483089.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20772..21005
                     /locus_tag="ACG827_000930"
     CDS             20772..21005
                     /locus_tag="ACG827_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0483090.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21104..21535)
                     /locus_tag="ACG827_000931"
     CDS             complement(21104..21535)
                     /locus_tag="ACG827_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0483091.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21614..22027
                     /locus_tag="ACG827_000932"
     CDS             21614..22027
                     /locus_tag="ACG827_000932"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0483092.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22065..23213)
                     /locus_tag="ACG827_000933"
     CDS             complement(22065..23213)
                     /locus_tag="ACG827_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0483093.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23335..23931)
                     /locus_tag="ACG827_000934"
     CDS             complement(23335..23931)
                     /locus_tag="ACG827_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0483094.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="ACG827_000935"
     CDS             complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="ACG827_000935"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0483095.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27161..27592
                     /locus_tag="ACG827_000936"
     CDS             27161..27592
                     /locus_tag="ACG827_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0483096.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27637..28986)
                     /locus_tag="ACG827_000937"
     CDS             complement(27637..28986)
                     /locus_tag="ACG827_000937"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0483097.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29056..30219)
                     /locus_tag="ACG827_000938"
     CDS             complement(29056..30219)
                     /locus_tag="ACG827_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0483098.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30348..30671
                     /locus_tag="ACG827_000939"
     CDS             30348..30671
                     /locus_tag="ACG827_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483099.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30854..32203)
                     /locus_tag="ACG827_000940"
     CDS             complement(30854..32203)
                     /locus_tag="ACG827_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0483100.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32272..33225)
                     /locus_tag="ACG827_000941"
     CDS             complement(32272..33225)
                     /locus_tag="ACG827_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0483101.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33327..34517)
                     /locus_tag="ACG827_000942"
     CDS             complement(33327..34517)
                     /locus_tag="ACG827_000942"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0483102.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34717..>34963)
                     /locus_tag="ACG827_000943"
     CDS             complement(34717..>34963)
                     /locus_tag="ACG827_000943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483103.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9952 a         7642 c         6120 g        11249 t
ORIGIN      
        1 tacctaaaaa tttggatttc aacgatagaa acactgtttt ttatgtaatt agtccaagat
       61 ttttctagac ttttgcttta ctattcggcc aaattggttt ttcttctttt tttcgttata
      121 atgaatatac ttatgaaaag aaatgaggaa tgaaccatgt cctttgatgg tgtatttacc
      181 cacgcgatgg tcaacgaatt gcgtgaaacg ttactatctg gccggatctc gaaaatccat
      241 cagccttatg aaaatgaaat cgtgctggtg attcgttcac gcggcaaaaa ccatcgatta
      301 ttattatctg cgcacccaag ttatgctcgt gtacagatta cccaaatcga ttatcagaat
      361 cctgataatc cgcctaactt cgtgatgatg cttcgaaaat atttggatgg cgctatttta
      421 gaagacatcg aacaaatcga aaatgatcga gtgatccatt tccactttgc taaacgaaac
      481 gagttgggtg atctacaaaa tatcatattg atcgttgaat taatgggacg tcatagtacg
      541 atcgtcttag ttaatcgaga aactggaaaa atactggatg cgatcaaaca cattggaagc
      601 tctcaaaaca cttatcgttc tttgcttcct ggtgtagaat atgttgctcc gccaaaacaa
      661 gaggtcttga acccatttag ctccgagaaa gaaaaaatct ttcagcggct ttcccaaact
      721 gaactagatc caaaagcaat ccaacgtcag tttcagggaa tcggtttcga tatcgctcaa
      781 gagctgacca aaagattact ggaacgtcct aatgaaaaga tggttgtatg ggacgaattt
      841 ttctcagcca tcagccatca gcctattcct actttttacg aaacggaaaa taaagatttt
      901 ttcacaccga ttgcttatca ggtattgtcc gaacaggcat ctgctgtaac agcttatcca
      961 acactcagtc aattattaga tagctattat cacgaaaaag cagagaaaga tcgggcaaaa
     1021 caacaaggcg gagaactgat tcgaaaaatc gaaaatgaac taaaacgaaa taaaaataaa
     1081 ttgaaaaaac gtgaacaaac actcaaagaa tccgaaaacg ctgagaatta ccgtagaaat
     1141 ggcgaactac ttactacttt tctgacacaa gtaccacgcg gcgcaaaaga agttgtttta
     1201 cctaattatt atgaagaaga tcggccaatc aaaatagcac tagatccggc attgacacca
     1261 aatcaaaatg cacagaaata tttccatcga taccaaaagc tgaagaacgc tgttaagctg
     1321 attggcgaac aaatccaaga agccaaagat gaaatccaat atcttgaatc tgtcttatct
     1381 caactggaaa ttgccggccc aatggatatc gaagcaatta aagaagaatt gacttcagaa
     1441 ggatacctga aaaagaagaa cttaaaaaaa caaaaacgta agaaaccatc tcagcctgat
     1501 caatactttt cttctgacgg aacgctcatt cttgttggta aaaacaatct tcaaaatgac
     1561 cagctgacaa tgaagacagc taaaaaaacc gactattggc ttcatgcaaa aaatataccc
     1621 ggttcgcacg tcatcattaa aagtgacaag ccctcagatg aaacaattac agaagctgcc
     1681 gagttagctg cctatttttc aaaataccga tactctgccc aagtgccggt tgatcttgta
     1741 caggtaaagc atatccgaaa accaaatggc gccaagcctg gttatgttat ttatgaaaac
     1801 caaaagacga ttatcgtgac accagaagaa gaaaaaatta cagctatgaa acaaaacagg
     1861 taatcaatga gtgtttctag aatcatattt gcgtactttc ccacaacagc aattagttat
     1921 aaattgtttg ctacaaaaag aggagttgtg acaaaattct gtcacaaccc ctcttttttg
     1981 atcaacggtg ttcaataaac agcgttctca tctgcttttc ttagctctgc tggtccagta
     2041 accgagtaag ctctttgata aatctttgag cgatttcact gagcggacgt ttcgccgaat
     2101 aaatgatata aaattcattt agttcgcctt ctgccaaagg gattaaccgc aagtgctcgc
     2161 gaatccctcc aatcacaata ccagatcctg aagcgtaagc atcagtttca agaacgatat
     2221 tcatcaaagt acctcgatca ctcgtataaa tcactgtttc ttgatcaggg atctcgataa
     2281 ggtcttcata aaaatacggg aaattatttc catcctgtgt aaaacgaact tgaggataag
     2341 ataacaattc ttctttggtg atctctgcgt gttcagctaa tggatgtcct tgccttaaaa
     2401 aaatatgcgt tttgaatttt ccaatgctgc ggccacttag ctctccttgt tgtaaatatc
     2461 ttctgatacc agcttgatta tgctcattca tataaataat gccgatctca ctgtgctgct
     2521 ctttgacttc ttcgatcact ctcgctgttg tcgtctccat gatccgaaat tgtttgatct
     2581 cgtctttgaa ttcctcgagt agctgtgcgg tcaaaggacc taagaaatca taatgctggg
     2641 cagcgatcga aaaattgtct ctcttttcac tcgttagata gtgagtttct aatgtatcaa
     2701 gctggaccaa tattttctcg gcacgattta agaagtcttg cccttcttct gttaaatatg
     2761 cccctcgatt cgttcgagta aacagcgtga tccctaactc ggtttctaat tctttgatac
     2821 cagctgaaag acttggctgg gagacaaaca ttttctttgc cgcttctctg aaactaccat
     2881 tgtttgctac agcgaccacg tatttcatct gctgaatatt cattgctcaa actcctcgaa
     2941 ataataaatt tctcttccat tcaagggctg gattggtcgg ttgttcggtc gagcatactg
     3001 accatcgaag gggagaagaa actcttggtt cagttttcct acatgatcca ttacaaacca
     3061 ttggattggc ccgctggtag gcggagttgt caatgagcca atataatgga aatggctttt
     3121 ttgctccgga aagaacacat ccggatttac ccaatgctca tgtttttcta agttccaact
     3181 ttccccattt tcttgattat acagccaacc ttcctgtacc attagctggt acatgacacc
     3241 cacaactaga ttctcttttt tgctgttcat atgaacaaaa tgaaattcaa tatcttcctg
     3301 ttttccgttg atgatatgtt cagaaggcaa gtggaaatga atatctgtca aataatagcg
     3361 ttctttcttg aactctacat aactgagttg gtcaaaaggt actaaatgaa tcgtgttttt
     3421 gaattccttg tcagtaaacc gttgcttttg ataatgaaag gaaagcgtgt ctccttctat
     3481 tttggtttct tcccccttgg tcaaatgaat aggcgactgc aatggaaatt ccgcacctct
     3541 cgcaaaccaa tcacataaac tcgcccaatt ttccggacct ttgtctccag aatagcccca
     3601 ttcgacatcc atattctttt tcaactatac ttccccctgc atctcatatc atttttattt
     3661 ttcttctttc tttacaatcg ttgagacggt ttctaaaaac gcctcttttc cataaaattc
     3721 ctggttcaat agttccggaa atatctcttt aaagaaatac tgaacagaag gccgatgttc
     3781 aaagctttta atcgtagtca ctgcctcaat gaacatctcc ttataaactt tttccgggtt
     3841 atttttgatg atttcatcaa aagattcata aagaaggtca accttatctg aaatggctaa
     3901 tagttctcct tctacggttt catcttttcc ttcgaacaac cgacggcgat aaacttcttg
     3961 gaactcagct ggaatctctt ggttgatgaa ttcgtccgtc atcttttctt caactgtttg
     4021 cagcatccct cttaactctt tgcttgcgta tttcaccggc gttttgatat caccaataaa
     4081 acgctctgtg tagtcatgat tcaatgattt ttcatagagc agtttccaat cgatcggttg
     4141 tccattcgct tcttcaatgt ctcctagaac ttgcgcaata gatgctacac gatacgaatg
     4201 tgctgcaact gtatgctctg tatatttaaa aaatccaggt gcccttgtga tcgtctctaa
     4261 attgttcaat cctaaaataa attcattcaa ccccataaaa gttacctccc tttgagattt
     4321 ctttttccga atatcatatc attaaataac tgatattcgc aacatttccg ctctttacca
     4381 tttttgaaac agctccctta ccaaaaaata gagaaaatct atatataata gaaaaaagag
     4441 aagagattat tctcttcttt ctgctaacca gtcttagaaa aatgaatttt aaaaaaatca
     4501 aaaactgatc acgatagaag tttcttttca tacctgatga aaagatgctt ctcgcaatca
     4561 gtttttggat ttttgctatt ccaattgatg attctggaga cacaaatcgg atttctctat
     4621 cttcatcaag cgcgcttaaa tctgccaatt ttcaggatct tttttccatt cttttagcag
     4681 agttaattct tgctcatcga tataacgatc ttctaaagct gcttcaatca atacagaata
     4741 attggtcaac gtcataagag ggatttcggc tttttcgaaa tttgcttttc cttttggcaa
     4801 ctcatacgta aagatagccg caacaccaag gatatccgca ccttctcttt ttgctgcttc
     4861 agctgcttcc aagacacttc cgcctgtaga aatcaaatcc tcgatcacaa ccatcttctg
     4921 accttcaaat atacgtcctt cgatttggtt acctttccca tgatctttcg ccttacttcg
     4981 aatatagacc attggcaaat ccagtatttc cgctacccat gcggcatgag ggattcctgc
     5041 tgttgcagtt ccagcaatca cttgtacttc agggtaagct tctttgattt tgcttgctaa
     5101 tccttttgcg atctcttttc ggacttttgg atagctcatc gtgattcggt tatcacagta
     5161 aattgggctt ttgattccac ttgcccatgt aaaaggatta cttggattta aaaatactgc
     5221 ttcaatttct aataagtctt ttgcgatact ttgttcaaca ttcattttag gttagctcca
     5281 ttctgtttga atcgtttgat atgctttata aggattagtt gactgagtga ttggtcgacc
     5341 tataacgata aaagttgagc cgatcttttt tgcctgagaa ggtgtgacta ctcttgtctg
     5401 atctcctgcg actgttcctt ctggtcttat acccggagtt aagcaaacaa attcgtctcc
     5461 tgccatttct ttgatcgctt ctacttccca agcagaagaa accacaccat ctaatcctgc
     5521 ttttttggca cagctagcat aatggatcac acttttttct aaagaaactt cgattagttg
     5581 atcggcatgc atttcttctt cacttgtaga agtaagctga gtgatagcta gaagtagcgg
     5641 acgttttcca ccttctggag tcccctcttc taatcctcgc attgccgcct ccatcatacg
     5701 aataccgcca gcggcatgaa cacaggtgag atcaacacca agctttgcca atccccgcat
     5761 tgctttttcg accgtattcg gaatatcgtg aagtttcaga tctaaaaata cattatgacc
     5821 agcctctttt agataacgta cgatttcagg tccttcttgg tagaacaatt ccattccgat
     5881 tttcacaaac aatttttctt ctttcgggaa aaatcgaaga aagtcttcta cctcttgcct
     5941 agttgaaaaa tctaatgcaa taattggtcg ttggtctagt tgattcatcg ttcacgttcc
     6001 tctctcactt ctttgattaa tctttccagc gattcgatgc ctagttcgct cattcgcaca
     6061 gggagctctt taattagttt aggacagata tacggatcgg tgaaattcgc ggtccctata
     6121 gctacagcac ttgctccagc cataaacatc tcaagaacat catccactgt ctgcacgccg
     6181 cccatcccaa tgattggaag gctggatata cttgctactt ggtgaatcaa acgaatagcg
     6241 acaggcttga ttgctggtcc ggagagtcca cctgtttgat tggctaaaat cggttttctt
     6301 gtcttaaggt caatccgcat accaagaaga gtattgatca tcgtaaaccc atctgcccct
     6361 ccagcttcga ttgcctgagc gattggaacg atgtctgtta catttgggga caatttgaca
     6421 tagacaggaa cagaagaaac tttctttact gcttgtgtta attgaaaagc aacatcagaa
     6481 tctgttccaa aagcaattcc gccatgtttg acatttgggc aagaaatgtt cagctcgatt
     6541 gctttcacat ttggtgcttc gccaattttg ctacaaactt ctacatagtc ttcttcacaa
     6601 gcaccagcaa catttgcgat gatgggtaac tcattgtact cctctaattc tggaagaagt
     6661 ttatgcataa caacgtctag accaggattt tgcaaaccaa ttgcattcaa catgccagat
     6721 ggtgtttccg ccactcttgg cgtgggattg ccaaagcggg catttggagt cgttgccttg
     6781 accatgatcg atccaagctg attcaaatca tagtattttc cgtattcttt gccaaaaccg
     6841 aaacagccgc ttgccggcat gataggattt tttaattcta ggccaggaag tttgaccgct
     6901 aaaggattct cattcatata attacctctc ctattttaaa aatcggtcct tcgtcacaaa
     6961 ctttcacact ttttgttcct gtttcatctt ccggcgtatg acaaacgcaa gcatagcaag
     7021 cccctatccc acatgccatg cgggattcta atgataactg gacattttga tgcgaagaaa
     7081 acagctgttc cactgtcttc aataacccgt ttgaaccaca tgcaaaaaca gctgtaggtt
     7141 ccgtcttcgt tcctaacaat aaattaccga catttccgtg cataccgtat gttccatcgt
     7201 cagtagcgat tcttgtttct cctagacgat taaattcttt ttcataataa acgacttcat
     7261 gtgtggcaaa acctaaaaaa tgcgttggct taattccttt ttcgactaac tgttttgata
     7321 actcatatag aggagggaca ccgattcctc caccgattat aaatacttcg tctcctgtat
     7381 gaagattgga caaatcaaaa ccattgccta aaggacccat gacatctaaa tggtctccgg
     7441 cagaaagagt agagaagact ctagtcccct caccttccac ccgataaatg atacggcaag
     7501 tatttgtttc gtggtcgata tgattcaagc taatcggtcg gcgtaatagc aagtctgcac
     7561 gcggcacgag aatatgcaag aactgtcctg gttgctgcat ttcttttact agatcacctt
     7621 tcaatgtcat ttcaaaaatc tttggtgcca gctgtttttg ctgaacgatt tccatcattt
     7681 cttgtttcat ctgtcccctc ctgcaacggt ttttcgtctt tttgtcttaa catgtgctcg
     7741 ccgttaaata gggttagttg aaaaagcacg ggattccatc actcgaacga tcgcatctgc
     7801 tgtgtctaaa gaagtaaaca atggaatacc atgttcaact gcttctcttc ggatgatgaa
     7861 accgtcttta gaagcatttc cccgatcttt atcaatcgta ttgacaacca cttgtgtttt
     7921 accatctctt attaagtcga ctacattttt ttctgctgct tcctcagaaa ttttcgctac
     7981 tgtttcaacg accagaccat ttttttcgaa ataagcagct gtatgtttcg ttcctatcag
     8041 actatagcca atttctgtaa aacgtttagc taattctagt gcttcttctt ttgtttcgtc
     8101 cgcaatcgta aataatacgg ctccatattc aggcaagtgt aatccagaag cttcgaatgc
     8161 cttgtacaaa gctttgtcta attgtctgtc agatcccatt acttctccag tcgatttcat
     8221 ttcaggccct aagtaagtat caactttctg caatttcgta aaagaaaaga caggtgcttt
     8281 aacatgaact tcttttgttt ctggataaag tccgttttca taaccaagtg tctttagttt
     8341 ctctcccaaa atagctttcg tcgcaatttg tgccattgga attcctgtca ccttacttaa
     8401 gaatggaaca gtccgacttg ctcgaggatt gacttcgatc acataaacct cattttcatg
     8461 gatgacaaac tggatattca tcatcccgac acaattcaaa cctaatgcta agcggatcgt
     8521 gtaatcttcg atcgttttct tgatcgattc agataatgtt tgaggtggat acacagccat
     8581 tgaatcccct gaatggacac cagcacgctc aatatgctcc atgatccctg ggatgagtac
     8641 tgtttcaccg tcacaaatcg catccacttc gcattcacca ccaagcaagt aacggtccac
     8701 tagtactgga tgttctggag atgctttcac agcatgttcc atgtaatctt ctaaatctcg
     8761 ctggttttca acgatttcca tcgcacgtcc gcctaataca tagcttggac gaacaagaac
     8821 tggataacca attctcgaag cgataaccac ggcttcttct ttacttgtag cagtatcccc
     8881 taaaggttga ggaatatcca actctttcag cgcttgctcg aataaatcac gattttccgc
     8941 acgatccaag tcttcgatcg ttgtccctaa gatttttaca ccagcagcag tcaaaggttc
     9001 ggctaagttg attgctgtct gaccaccaaa ttgaacgatt actcctatcg gctgttctag
     9061 ctcgatcaca ttcatcacat cttcgaaggt caatggctca aaatataatt tatccgaaat
     9121 agaaaagtca gtcgaaactg tttcaggatt gctgttcatg atgattgctt cgtatcctgc
     9181 ctgctggatt gctttgacag aatgcacagt cgcatagtca aattcaaccc cttgtccgat
     9241 acggatagga ccagatccca gtactagaat tgagggcttt tctgatcgtg aactttcatt
     9301 ttcatattca taggaactat aaaaataagg tgtttttgat tcgaattccg ctgcacaagt
     9361 gtcaaccatc ttatatacag gaatcaaatc attttctttt ctcatctggc gaacggcttc
     9421 ttcgtttatt tcccacagct ccgccacttt tttgtctgta aaaccgtttt gtttcgctgt
     9481 tttcaatatc ttgtcatcca acggctgctg tttcaactgc tgttcgattt ctacgatatg
     9541 caacagttta tctaaaaaga ataaatcgat ttttgtcaaa tccgctaatt cttctatggt
     9601 gtaccctctt ctaatagctt cagctacata aaacaggcga tcatcttgtg ctcgaaccac
     9661 tttttggatt aggtcatcgt ccgtgatgtc tgccaactca ggaagttcga tatggtgtgt
     9721 accgatttct aaagaacgca cagctttcaa taaagactct tcgatattcc gaccgatagc
     9781 catcacttca ccagttgctt tcatctgcgt gcctaaaagc cgttctccgt tttcaaactt
     9841 atcaaacggc cagcgaggaa ttttagctac aacataatct aatgcaggtt caaattctgc
     9901 aaaagtcgtt cctgtaacag gatttttcat ttcgtctaat gttaatccaa gcgctatttt
     9961 agcagctagc ttagcgatcg gatagccagt cgccttactt gctaacgcag atgaacgaga
    10021 aacacgcgga ttgacttcaa tcacataata attaaaacta tgtggatcaa gagcgagttg
    10081 gacattacag cctccctcga tcttaagtgc tcgaataatc gaaagagaag catcacgaag
    10141 catttgatat tcatggtcag acaatgtttg actaggggca aatacgatag aatcacctgt
    10201 atggataccc accgggtcaa aattttccat attgcagaca acgattgcat tatcagccga
    10261 atctctcatc acttcatatt caatttcttt aaaacctgca atacttcgtt caatcaaaca
    10321 ttgggtagcg ggggaaagac ttaatccatt ttctgatacg atacgtaatt cttcttcatt
    10381 ttcacacata cctccaccag ttccgcctaa agtaaaggct ggacgaacga tgacaggata
    10441 gccgatttta tttgcaaaat ctacagcttc ttctactgta ttgacgattt cagactcggg
    10501 gattggctga ttcaattctt tcatcaactg tttgaataga tcacgatctt ctgcttgatc
    10561 gatcgcagaa agctttgttc ctagaagttc tatgttcaat tcttctaaaa tacctgatgc
    10621 tgccagctcc attgccatat tgaggcctgt ctgtccgcct aaagttggaa ggagagcatc
    10681 tggacgctct tttcgaagaa tccttgatac aaattcaagt gtgatcggtt cgatatatac
    10741 ttgatctgcc acttctttat ccgtcatgat cgtcgcagga ttagaattta ctaaaaccac
    10801 ttcatatcct tcttctttta aggcaaggca ggcttgtgtt cctgcgtagt caaattctgc
    10861 ggcttgacca atgatgatcg gaccagatcc aatcaccata attttcttga tatctgtacg
    10921 tttcggcatc tgtttactgc tcctttcctg cgtccatcat ttccataaat tcatcaaata
    10981 aatgtagtgc atcatgcgga ccaggggcag catctggatg aaactgcaca ctgaaagcgg
    11041 gataatctct gtgtttgact ccttctactg ttccgtcatt tacttctaca tgagttacga
    11101 tgagtttttc tggatcaatc gtggcttcat cgactgcata gccatgattt tgagaagtaa
    11161 aatcaattct tccggtcgct acttctcgta ccgggtgatt taatcctcga tgtccgaatt
    11221 tcattttata ggtatctgca ccgttggcta aagcaaataa ctggtgaccg agacaaatac
    11281 caaaaatcgg tacttttcct tggatttttt tgatcatttc gattgcttct ggcacatctt
    11341 ttggatcacc aggtccattc gtcagcatga ctccgtctgg tgatagcgct aaaatcgtct
    11401 cggcatctgt gttataggga agtactgtga ggttacagtt tcttttactc aattctcgta
    11461 aaatactatg tttcaagccg aagtcaatga caatgacttt ccgacctgtt cctggacttg
    11521 gataaggttt gttagttgag acttgctgca cttgattggt tgctagaacg gttgctttca
    11581 gctgatcaaa tgcatgttcg aaggaatctc cagcatcaac tatactagct ttcatcgttc
    11641 cgacttcacg gattttgcga gtaagggcgc gcgtatcgat acctgatatt ccaggaattc
    11701 ctttttgttt caaaaatgaa tctagcgtca tttggtttcg ccagtttgaa gctacacgtg
    11761 catgttcttt tactactact cctttgcatg ttggagaaat cgattcataa tcatctctat
    11821 ttaccccata attacccacc ataggatagg taaatgtaat gatttgaccg ttaaaacttt
    11881 gatcagtgat agtttcttga taaccagtca tacttgttgt aaaaacgatt tctccgaaga
    11941 cactagcatc tgcaccaaaa gcctttcctt cgaaaactgt tccatcttct aatataagca
    12001 aacgttccat acttttattc tcccttctgc cacacaagtt ttccgttgac aaaagtcatt
    12061 tgtgtttcgc cttttacttt ccagccgata aaaggtgtat tctccccttt tgataaaaag
    12121 tcttctttat cgatcgtgca agtttgtgta atatcaaaaa ctgctacatc agcgggggca
    12181 ccaaccgtta gcgttcctgc attcaaacca aaaatctcag ccggtttaac tgccaaccaa
    12241 ttgatcactt gttccaacgt gaatcgacca gtttctacaa aatgagtata gatcagttga
    12301 aacgctgttt cgcttcctac gataccaaat ggtgatttca tgaaactttg gcttttttct
    12361 tctaatccat ggggggcatg gtctgtagcg atgcaatcga tcgtaccgtc aagcaagcct
    12421 tcgatcaaag cttctctgtc ctcccgtcct cttagcggcg ggttcatttt ccaaaagccg
    12481 aaatcttcgg gaatatcttc gtcgatcaaa ataagatgat ggggtgaaac ttctgctgtt
    12541 acgtggattc ctgctttttt tgcgtctctg attacacgga cactttcttt agtagaaaca
    12601 tggcaaacat ggtaatgaac accagttgct tcagctagca acagatctct agcaatttga
    12661 gaagactcgg ttacgcttaa gatcccggga agaccaagtt cttctgcctt cttgccagcg
    12721 tgcataacgc cgccaaataa taaagattca tcttctgtat gggctactag ggctttattg
    12781 ttttttgcag cttctttcat agccagatac atcgtcccag ctgtttggac acctacaccg
    12841 tcatttgtga aagcaaaagc accttcttca atcagtgctt cttgatcaac taatttctca
    12901 cttcgcagat tttctgtgat tggcgcatac tgcaatacct tcacaactgc atcttttcta
    12961 atgatgtcat atacttgtct cagcttttct gccgtatcag gtacaggatt taaattcggc
    13021 atcgcgcata cagtagtgaa accgccacga gcagctgagc gagtcccagc ttcgatcgtc
    13081 tccttgtatg taaatcctgg ctctctcaaa tgaacatgga cgtccactaa accaggtgtg
    13141 atcaattgtc cctttgcatc aaacacttca tcaaattcgg cttctgagaa tcctgtaccg
    13201 attgctttga ttcgcccatc ttctatccaa atttctgctg gtgtcgtcat attttttcgt
    13261 gtattgatct gaccgttttt aatgagtgtt ttcatttttt catcctccta taattacgct
    13321 tttccattta gcactgcttc taagattgcc attctgacga atactccatt gctcatttgc
    13381 tgaacgatcc gcgactgtaa tccttccact aatgaatcag ctaattctac atcacggtta
    13441 actggtgctg ggtgcataat gattgcatgt ttttgcaact tcttcgcacg ctcaactgtt
    13501 aacccatgtt cctgatggta gccttctttt gagaaacttt cttttccatc atggcgttcg
    13561 tgttgcaccc gaagcatcat catgacatca acttgatcta gtagttcatc caatggcata
    13621 taatgtccgt acacttcgaa ttcatcgtcg taccattctc taggacctga gaagaatact
    13681 tgtgcgccta gacgttttaa catctgcata ttggatttag ctacgcgaga gtgagtgata
    13741 tcacctacga tggcaacttt cagaccttca aaatgaccaa attcttcata aatggtcatc
    13801 aaatcaagca agcattgggt tggatgctgt ccgcttccgt ctcctccatt gataatcgaa
    13861 cactggattg ttttactttg tatcagttcg tcataatagt tctcatcacc atgacggata
    13921 accgctacgt ctacgcctaa tgctgacatc gtcagtactg tatcgtatag tgtttcccct
    13981 ttttggacag agcttgtgct tgcttcaaat tcaatcactt ctagccctag ttttttctcc
    14041 gctacatcga agcttttatg tgtgcgagta ctattttcga aaaagaggtt tgtagcaaag
    14101 tactggcgct tttgcggagt ccaattagca ccttgcttga attcctgccc tctgcggatc
    14161 aagcccataa cttcttgatc agtaagtgcc tcaacggtca ataaatgctt taagctgata
    14221 cgttctgatt tgatgatcat aatgtttcct ccttagtttt cttcgcttcg tgaagtttct
    14281 ggtaagataa gatttaaaat gatgccaagt acagttgcta atgccattgc cgacaatgta
    14341 aatgtgccta cttcaaagac gagtccgcca attcctacta ctaagataac tgaagcaatc
    14401 aacagatttt tctttttatc aaaatcgatt ttgttctcga ttaaaatctt cattccactt
    14461 gcggcaatta ctccaaataa gataaaactg attcctgaga tgactggtcc agggatactt
    14521 aaaatcaaag cgcttaattt tcctacgaat cctaaaatga ctgcaaatac tgcagctcca
    14581 ccgatcacaa acacgctatg gacacgtgtg atagccagaa caccaatgtt ttcaccgtaa
    14641 cttgttacag gaggacctcc gacaaaggct gccacgattt gtgctaatcc atcacctgtc
    14701 aacgtcttat gaagacccgg ttcttcaaag aaattacgtt tagttagttt attcaatacc
    14761 ataagatggc cgatatgctc tgtcatcgtc acaaaagcga tgggtgccat tgttgtgatt
    14821 gctccaagat atagctttgg ttcatattgt acaaagggaa tctcaaagtt aggaacagca
    14881 aaccaggcag cgtctacgat tggcttagta tccacgattc caaacagcaa ggctacgatg
    14941 tatccgctaa taatacctag taaaatcgga atcaatccga gaaaacctct tagccacatg
    15001 ttgaagaaaa ttgttaatcc taatgtgatc agcgctacag caatatactt gaaatcatac
    15061 gtgccctgat tatacatagc atttgttgct gcatttgccg caagtcccaa cccgataacc
    15121 atgatgactg gaccgacgac gattggaggc aagattttat ccagccatgc tgaaccgatc
    15181 tttttgatga tcagcgcaac aatcaaatag actaatccag ttgtgactgc tccttgtgca
    15241 atcgctggat aaccatctgt tttcatcaaa agctgcatcg ctgcaatgaa agcgaagctg
    15301 cttcctaagt acgcaggtat ctttccttta gtagtaaata agtacaccaa tgtacctagt
    15361 cctgaactga ctagcgcaat acctggatcg atacctacta agatggggac taacacggtt
    15421 gctccgaaca tcgtgaacaa gtgttgtaaa cttaatccta cccaatgtcc tggggttggg
    15481 cgatcgtgga tatctaacac cacgtcatca ttatgaaatc cttttttctc cgacatgttt
    15541 ctctctcctc taatcttcct ctttcaaaat catgatgcga tcttgtccat caagctcttg
    15601 catctctact aaaatctctt ccgcccgaga tgttggaatg ttcttcccaa cataatctgc
    15661 acggattggc aattctcgat gcccacgatc gaccaggact gctaatgaaa tttttcttgg
    15721 tctgccaaaa tccattactg cgtccattgc tgctcgaatc gttcgtcctg tatacaaaac
    15781 atcatcaatc aaaataactt cttttccttc taaggaaact gggatgtcag aagaatgaag
    15841 ttccggttct tctggatttt cttttttgtc atctcggtat aaagtgatat ccagttctcc
    15901 tacaggaata tcgatatctt ctagctgctt caggcgttcg gcaattcttg atgcaatgta
    15961 gattcctcgt gttttgatac ctaccaatac gatatcttgg atactatggt ttctctcgat
    16021 aatctcataa gtaattcgag tcaatgctcg cttcatggtt acctgatcta ctacttcttt
    16081 tgcttgcatt ttcattcccc ctactttttt gcataaaaaa actcctgccc taagaggaca
    16141 ggaggaatgt ttagactgag cgtatactga tacactgata gctatactat gtcaatgagc
    16201 aatctactta tggcgcacca tacgtatcct tgctcccacc cttcttagcc tcacgggact
    16261 aaacttaaag gaattctatt aagttttatg ctttcgttgt taattactat accgttatct
    16321 aaattaaaat gcaatgtttt ttcttaattt ttctaaagtt ttttcaaaaa cttctggaag
    16381 aggtgcttca aagatcatct tttcgtttgt agtcgggtgc gtaaaaccta acatttgcgc
    16441 atggagaaac tgaccattgc ctttcaaagt tttcttcggt ccgtatactg gatctccagc
    16501 taacggataa ccgatatact tcatatgcac acggatctgg tgtgttcttc cggtttctag
    16561 ttttaattca accagagtaa atccttcgaa acgttccaat acagtaaaat gtgtgacagc
    16621 cgacttccca tcttctctta tcgcctgcat tttccggttt tccttcgaac ggccgattgg
    16681 cgcattgatg gttccttttt cgtgaggaat ctctccatgg actaaagcaa catatttgcg
    16741 caaagaggtc ttatctttta attgctgagc taatgattcg tgtgcctggt catttttggc
    16801 aaccattagc agtccggaag tatctttgtc gattcggtga acaatacctg gacgaataac
    16861 gccgttgatc gtagaaagat ccttgatatg gtacatcagc gcattgacca ttgtgccatg
    16921 cgtatgacct gctgaaggat gaacgaccat tccttgaggt ttgtttatca ccgctacatc
    16981 ttcatcttcg taaacgatcg tcaacgggat attttctggg accacatcta atgtttctgg
    17041 ttcggggatc aagacagtaa tctcgtctcc ttgttttact ttgtaatttg ctttgactgg
    17101 ctgtccattg acagaaaccg ctccattttt cagccacagc tgaatttgcg aacgactgta
    17161 atcggtgaat aaattagtca atactttatc gatcctacca gattcatggt gaatcgtcgt
    17221 tttaagttct gtcatttttt tctttgatcc ttttcatcta aaataatata aattaacata
    17281 caaatcactc cacatacaag tgtcacatct gctacgttaa aaatcggaaa gctcataaaa
    17341 tctgtctgga acatatctac tacatagcca agacgaatac ggtcgataaa attgccaatc
    17401 gcaccagcta gaatcaaact caatccaatc atgaaccagc gattaccttt ttcacgattt
    17461 ttaatcatca atgtgagaat cacaacgact gcaatgagtg tcactatagt aaagaaccac
    17521 atcttccctt ctagcatact ccatgcggct ccagtgtttc gaatatgatt cagtgacaaa
    17581 atacctggta taagattttt cgtctctcca agcgctagat tatcagtgat ccaccatttg
    17641 accaactgat ccactcctac tagcaaagcg ctgaatatcc aataaataat taacaaaaaa
    17701 tcaatccttt tctatttgtt tataaatatc acgaggtcgt acgatcccga gcagattacc
    17761 atcgggattc ccatctttag tgataagtac ggcttccaaa gtcccttgct cacgaaacat
    17821 cgcttcaacc tcgtaaatat gtgttttagc tggaacaaaa tgataatttg tgtactttcc
    17881 gttttggatg atcaaatcca acgctcgttt atttgttaaa tcaaccggac ctttttggct
    17941 ttcttttgct agccaaaacc cgatattacg taaagtaatc aatgctacaa attttccttt
    18001 attgtataac ggaaattgtg aatacctttt ttctgctact atctttaaca gttcaagtaa
    18061 cggaagatct tgttcaaaac ctgttacacg ttttgcaaat cgaggcagca cggtttcagg
    18121 ctgaatcagt tcttgttcga tcagctggat tcgcttgact gtccaatcat ttggctctgc
    18181 aatgacaaaa tcgactgcga tccgatcatg gacgatcgca ttccttaact gagcaagctg
    18241 taaaagatca tcttcgtatt ttctgatcat taattgtttt ctttgagcta ggcgtctgac
    18301 aagttcggta aatcccatat ttcgtgcatt tcccatctcg tctctcatcc atttttcgat
    18361 tcgattgaaa ctgattaaaa aaagatcaga gtttgccata attttcctcc ttgatcaaat
    18421 atgcccggtt ttttatatag aaaaagaacg caatcaacac aataactgca aaaaaactga
    18481 aaaataaatt ttgtaggaaa acggccccca acccagttgc tagaaataaa aacggataga
    18541 tttgaagaat caagtaattc attgtgatca cataagcagg ctttgcttct ttcactgtat
    18601 aaacggggct ttttagccaa taaaaggtga cctgcatcgt aaagggcact tccagatcaa
    18661 gcgttatctt ttgttcatga ctgagaacac tgcgtttttc attgttgatt cttagctgaa
    18721 tcgagctacc cataccgtaa aaaccggttt ttcttataaa tgtcgttttc atcataccct
    18781 cctattatgc tttagtatag ctcaaaatac aataaagaaa aagaatgatc ctagtgtaaa
    18841 caaaattaat tccctatcgt ctaaaaaaag aagctgtagt caaaatacag ctccttcgat
    18901 ggtagattat aatcaagctt aaaacaatcc caacacttct ccgtcatttg tcacgtccat
    18961 attcaatgca gcaggttttt taggcaaacc aggcatcgtc ataacatctc cagtcaaggc
    19021 tactaaaaat cctgccccta attttgggat gatttcgcgg atcgtgatgg tgaaatcttc
    19081 cggtgcacct agcagtgaag gatcatcaga aaatgaatac tgtgtttttg ccatacaaat
    19141 cggcagtttg tcccatccat tcttcgtaaa ctcttctatt tgtttttgcg cttttttgct
    19201 gaaaacaaca ccatttcctc cgtaaatttt cttgacgatt gtttctgtct tttcttgtat
    19261 cgtttcttct aggcggtata atggcttgta attagcttct tttctatcaa tcaatcgtac
    19321 tacagctttt gctaaatcaa cgccgccttc tgctccatct gcccatacac tcgctctctt
    19381 ggcaagaatc ccctgatttt cacacaagtg ctccaacagt gtgagttcat tgtctgtatc
    19441 tgttacgaat tcattgattg cgacgatcac aggtagctga tattgttcca tattacgtat
    19501 atggcgcttt aaatttgcaa aacctacttt caaagcatca agattctcat ttttaagttc
    19561 atcttttttc attccgccat gcattttcaa tgcacggaca gtggcgacaa tcaccacagc
    19621 atccggcgct tttttcaagt taggtacttt gatatctaaa aatttctctc cgccaagatc
    19681 tgcaccaaat cctgcctctg tgacaacgta atctgctaat ctcaatgctg tcttggtagc
    19741 taaaatacta ttgcagccat gagcgatatt ggcaaacggt cccccgtgga caaaagctgg
    19801 tgttccgtaa attgtttgta caagattcgg cttgattgca tctttcagca gtaatgccag
    19861 agcgccttct gctttcaagt ctccagcagt cacaggctga cggtcaaatg tatagccgac
    19921 aacgatcctt gcaagacgag cctttaaatc ttcaaggtct gccgcaagac acaagatagc
    19981 catgatttcg ctggcaactg tgatatcaaa cccatcttct cttggaacac cttggattgg
    20041 cccacccaag ccaacaatta ctttacgcag ttcacgatca ttgagatcga ctacccgttt
    20101 ccaaatcact cggcgggcat cgatgttcaa ttcatttcct tgatgaatat gattatctaa
    20161 aagagcagat aatgcattat tagctgttgt gatagcatgc atgtcaccag taaaatgcaa
    20221 gttgatatct tccataggca atacctgtgc atatcctccg cctgcagcac cacctttgat
    20281 tcccatcaca ggaccaagtg agggttctcg caatgcaata acagattttt tattgatttt
    20341 attgagcgcg tctcctaacc ctacagtgat cgttgacttt ccttcgccag ctggtgttgg
    20401 attgatggaa gtaactaaga tcaaatgccc ctcagcgtta tcctttgatc gattgattcc
    20461 agaaaaattg attttcgcct tatatttccc atacagttcc aaatcgtctt ccttaagatc
    20521 taatttacta gccacttcaa caatcggttc tagctctgtt tcttgtgaaa tttgcagatc
    20581 attcttcatt tgattacccc ttttcacgaa ctttattcaa caaaatatct tctttgttaa
    20641 gatatgccaa ttataccttt ttttattcag aaaaaaaaca taaaattaca ggtttttaat
    20701 ttatttgacg tacttataaa tactctttat aatgaaggct atgatcagac tttagaaagg
    20761 tcgtgagtgc tttgaccaca ggaattgtaa aatggtttga caacaaaaaa ggatacggct
    20821 ttatcagtta tgatgacaca gaagaaattt ttgtacattt tactgcgatt gaagaagagg
    20881 gattcaaaac tttggaagaa aatcaagtgg tcgaatttga gatcatcgaa gggaatcgag
    20941 gaacacaagc tgcccatgtc aagaaaggca acgttcagac taccgatact agtatgcatg
    21001 aatagataaa caaatagagt gagacgctaa acgggatgaa ccgtacgtct cactcttttc
    21061 ttttcctgta aaaattcgtt tttttaggtt ctgattttta aaattagctt tttctttttt
    21121 tcgtataagg ataaggaatt gattctgatt tgggtccgtt tttttcaaaa tgatcaaaaa
    21181 ggactacttg atcttttttt cgcatcatga aaacagatgg ggctttctca tcttgcaaca
    21241 tcatttgcac cccagtatta gttggcgtca aagatcgaat ctgtccgaca gatatttctc
    21301 ggtctttcca aaaagcagca taacgtataa tcacagagtt tgattttaaa ataaaagaac
    21361 gccgcaaacc tagaaataga aaaacaagaa aaatacctaa aacaaaattg cttttccaat
    21421 aaggacctgt gttttctaaa gaaagaatca gactaaaaaa cagaatgatc aaggtcaatg
    21481 accaatagat gatcgtatgt gccagttcag gctgccatcg aatatttttt ttcattctct
    21541 aacctctcac cgattcagta atgatataat cttaccataa cattagtttg aacaaaaagg
    21601 agaatctctt tttatgttaa aagtatacat tgatgcctct acaaaaggca accctggtcc
    21661 tagtggaggt ggaatcttga ttgtctatca aggtaaacaa gaacagctat ctatcccgct
    21721 ttctataggg acaaaccatc aagcagagtt cgaagtcttt ttaaaaactt tagaatttct
    21781 aaagaaaaac gacttacaga atgaaacgat tttctgtttt tcagacagca aaacactagt
    21841 ttctacggtc gacaaaaacg caacgaagaa cgagctcttc cttccctacc ttcaaagaat
    21901 ccaagaactt ctatccgaat tttctttatt gatcctgcaa tggttgcccg aaaacaaaaa
    21961 taaaggagca gataatcttg cacgtcaggc tctccataaa caattaaaag aaaaaaagac
    22021 tctttaaatt aaaaacttta aggagtcttt ttatagtttc tcatttaata taaaagataa
    22081 ttgtgatcaa acgtaatttc caattggtaa tctccgattt tttcttctat ttgactacgt
    22141 aagattcttt ctagttctgt atcaacaaac tggaatttct cagggaccac tacatcaaat
    22201 tgcagcgttt ttccttgatg aattactcta aaatcatgaa tattaagccc tgtacctgtt
    22261 tcaagaagaa tttgctttaa ctgccgcaag atattatagt atttttcgtt atcaacaggc
    22321 attggatcca agtgacagac aagttccaca tccaaagact ttttaaagtc tttttctatc
    22381 agatcaataa tcttatgagc cacattcaaa ttcattgatt catctacttc gatgtggact
    22441 gatgcaaagc gtttattggg tccatagtta tgcaccaata aatcatgata gccaagtata
    22501 tcatcgcaag catttaaatg ctcttccatt tcttcaatct ctgctggcgt tgggcgactt
    22561 cctaatagtt cgtatacaaa atttcgaagc atcgtcaatc cactataaag gatatataat
    22621 gctaaagcaa aacctatata tccatcaata cgccatccag ttacccattc gattgctgct
    22681 gaaagcaata cagccagtgt agtaaagaca tcattataac tatctgttgc tgtagctctc
    22741 aatgtttcag aatcaatgga ttgcgcaata acctgataca ttttgccttg tgcaaatttc
    22801 aaaaagatgg aaatgattag gacaaaaaag acgatgggtg ttaaagagac attttctgga
    22861 tggatgattt tttcagcaga agactctaaa aattgaaatc ctacataagt aatgatgatc
    22921 gaaacaaata acccactgat atattcgaag cgttcgtgtc cataaggatg ttcctgatct
    22981 gctggctttg cagcaatttt aaatccgatc aaagtcaata tggatgaagc agtatctgat
    23041 agactgttca ctgcatcagc cataatggag acacttcctg aaaaaagccc gatcattagc
    23101 ttagagacaa acaagatcag attcgtaacc aatcctaaga tacccgctag tactcctttt
    23161 tttgtccgct gttgcggtat ttgtttcttt ttttcttctt gtttcttagc caagtttttt
    23221 accccttcta gtttgcttgt tgctcaggta aaaagatgga gaggacgatt gttttcctct
    23281 cctctcttcc tttttctatc tatcgtgcgt cctattacta ttaaaagatg gtcattattc
    23341 tggtttcaac ggtgttgctg caaataacgc ttttgctagt aatggtgtta tgattcccgc
    23401 acctattgct tctggtatcc cattgattgc tgcaacccct gctaaggttt taaataaaag
    23461 agcagagtct acaccatagg catctgccgt tgcacctgta taaaataatc ccatgaatcc
    23521 taacactaag actgtattga ccaatgaacc cagtacccct gcaaaggctg agctgatcgt
    23581 tattgattta tttttttgat aaatccagtg gaaaaccaag cctgctacta agccaacaag
    23641 tactctggga actaccgaga taatcggatt cgtgaacact aatgtatcaa atggcgttgt
    23701 tggcatggca taggcacgaa caattgtagc gattccccaa aacaagccaa tcaccatgcc
    23761 atcttttggg cctaatacta ctgcagcaat aattactgtg atatggataa ttgttaaact
    23821 aatgaatcct aatggaataa accccagcca aggaaccatc gcttgtacga tgatgattgc
    23881 taacaagatc gcacgtaaga ctagtcgaaa ggtcttattt ctactattca ttttttcacc
    23941 cctttttatt tatactaatc gtaaaaattt ctgtcacttt cgtcaactaa agaccatccg
    24001 aatccgacca tttgattaga tgcgccatcc aattgaaaaa agtatgaggg caagacttgt
    24061 ttgtcttcgc ccccataatc atgtttcgtt caatgtttga acttttgttt ttctgtcaaa
    24121 cgtaaacgcc ctattgttgc gcaacccatt ctttcgcagc tttaagtgca tctggaatac
    24181 cagcaggatt tttcccgcca gcttgtgcca tgtctggacg tccaccaccg ccaccgccta
    24241 ctttcggtgc gattgcttta atcaagtcgc cagcttttag tccagcttca tttgattctt
    24301 tcgtcattgc cgctagcaga ctgacttttc cttcttgttc gtttgctaaa acaagaatat
    24361 ctgaaatatt tttttgtttc cattggtcag ctaattgacg cagctgattc atatctttca
    24421 cttttacttg tgctgcaatg attgtttttc cattgatcgt ttctggatct ttgaatacat
    24481 caccagcttg ttgattcgct aacttgctag ctaattgttc attttccttt tgtagctctc
    24541 ttacttgctg ttgcaattgt tccgcttttg aaacaacttc tttcaattga ggtgatttaa
    24601 caattgttgc tacttgtttt agttgttttt cttcttcttc aagtagttcg tacgcttctt
    24661 tacttgttac agcctcgatt cgtcgcacgc ctgcaccaat tcctgattca gaaacgattt
    24721 tgaagattcc gatgttttct gtatttgcta catgtgtgcc tccgcacagt tcgattgacc
    24781 agccgccaat atttactact cgaacgtcat gtccgtattt ttcaccaaac aaagccattg
    24841 cacccatgtt tttggctgta tcgatatctg tttcaattgt ttcaactggg atagcttccc
    24901 agattttttc gttgacgatt ctttccattt cagctaattc atctgaagtg atttgaccaa
    24961 agtgggtaaa gtcgaatcgt aaatgtccag gagcgactaa agaaccagct tgatttgcat
    25021 gctcgccaag tacttctttc aatgccttgt gcagtagatg ggttgctgta tggtttttga
    25081 tgatccgatt gcgcatcgat tgatcgatca atagatgata ggtttgtcct tcactcattg
    25141 gtgcttttac ttctacggtg tgcataaatt gaccattagg cgctttttga acatctgtaa
    25201 caactgctac gatttcgcct acttcattca tgatccatcc ttgatccgct acttgcccac
    25261 ccatttctgc atagaaaggt gtttgatcaa agatcatttg agcttcacct tctgcgactt
    25321 cattcaaaag agactcacct ttaacgatga ccaataattg actatctgat tcttgttctg
    25381 tgtatccaat gaatttactt tctactttga tatctgtcaa taaagcagat tgtaccccca
    25441 ttgatttttc agtactgcgg gcagagcgag cacgttcgcg ttgtgcatcc atttcttttt
    25501 caaaaccagc atgatcaact ttcagtcctt catcttctgc cacctcttcc gttaattcta
    25561 ccgggaagcc atatgtgtcg tatagtttga agatgtcttt tcctgctaga gtcgtcccgt
    25621 tttcgttttt tacttcttgg atcacttgat ttaagatttc caaaccttca ttgattgttt
    25681 cattgaaacg ttcttcttct gtacggataa ctttttcaat aaattctttt tgttgaagta
    25741 cttcaggata ataacttacc atgatttccc caacaacagg gactaaacga tataagaatg
    25801 cttgttcaat acctaatttt ttcccatgca tgacagcacg acgaagcaaa cggcgtaaga
    25861 cataccctct cccttcattc gatggcaatg caccgtcgcc aatagcaaat gaaagggcgc
    25921 gaatatgatc tgcgattact ttgtaagaaa tatctgttac tgggttttca ccatatttta
    25981 cttgtttgct tagcttttct acggcgtgga taataggcat aaacaagtct gtctcaaagt
    26041 tcgttgacgc atcttgaacg attgagacca tgcgttccaa tcccatcccc gtatcaatgt
    26101 ttttatgagg aagtggttca aatgtgttat caggcttatg gttgaattct gaaaatacta
    26161 agttccagat ttctaaataa cgttcatttt ctcctcctgg atagttttca ggatcgtctt
    26221 ctgctaaatc gttataagct tctccacggt catagaaaat ttccgtatcc ggaccacatg
    26281 ggcctgcacc gatatcccag aagttgtctt ctacatcaat aatgtgatct agtggcaggc
    26341 caacttcttc atgccagatg cgttttgctt ctgtatcttt tggataaact gttacataaa
    26401 gtttttctgg atcgaatgcc atccattttg gatcagtcaa aaattcccat gcccagtgga
    26461 tggcttcatt tttgaaatag tcaccaatcg aaaaattacc cagcatctca aacatcgtat
    26521 ggtgccgtgc tgttttaccg acattttcga tatcatttgt tcgaatagat ttttgggcat
    26581 ttgtgattct tggattttct ggcacaactg aaccatcaaa atattttttc aatgttgcaa
    26641 caccagagtt gatccacaat aaggttggat cgttcacggg gatcaaagac gcactaggtt
    26701 caatcgtatg tcctttagat tggaagaaat caagaaacat ttgtcgaact tgtgcactac
    26761 ttaattgttt catgtcatta cctgctttct ataaattaaa tacaaaaaaa caccatcaca
    26821 gccaaggacg aaaactcgcg gtaccacctt gattgcaatg atgtgtaatc attacctctt
    26881 aaactcgata acgctgagct gcgtcggtat ttctaccaaa atagaaaaag ggagcaattt
    26941 gattagtgac tattcttttt tcagccaaga aagaattttc tgtgagtctt aaaaaacaaa
    27001 tcatatcctt ttcgtactta attagtatac gcattctatt gatcactgtc aatatctcaa
    27061 tttttatttt catttccttt tggtcatact ccacgatttt tggtacgata agaataaagg
    27121 aatcaataca tctctgctat tcatgacaag gaggaatatg atgaaaaaaa tagttggagc
    27181 ggatgaatgg atacgcgcag ctagctgctt tttgagatat caagtttttg tattagaaca
    27241 agggattcct gcagaactag aattcgacgg aaaagatatc ccgcaacaaa cctattatct
    27301 atttcttgat caaaaaagac cagtagctac tttgcgttat gaaaagaaaa cacctacttg
    27361 cttcaagccg gaacgatttt gcgtagcaaa agaataccga ggaaaagggt taggcggtca
    27421 gttgcttgaa gctgctgaaa aacgggcggt ttcagaaggt tgtcagacta gttatgtagt
    27481 agctgaaatg acggctgtag atttttatga aaaacaaggg tatcatactt gcacagaacc
    27541 ttttattgaa gatgaagtac tttgtatcgg aatgaaaaaa agtctagctt aaaaacaaga
    27601 gattgtgaca cgccttttgt cacaacctct tgttttttat ttatgtgaat ttttcttatt
    27661 tttacgagca gtacggcttt gctgtctgcg ttcgattttg cgttcttttt tatttttttc
    27721 tgcaattgcc caatcgattt ttttcttata accaggcttg atctttttct ttttcttctt
    27781 cacaagacca atcagcgtgg gatctaatac atcttttgat ttttctcgtt tggcacgtct
    27841 attacgatcg tatgttttga cgatttcccc gtttttgatt tcttttggtt caaatgagac
    27901 gcccaatttt tctatcgcat caatggcttg ctcatcattt ggcgaatata acgtaatggc
    27961 cgttcctttt aagccattac gtccagtacg tccgacacga tggataaaga agtctagatc
    28021 ttctggaatt tctgcattaa taacatgaga gacaccttcg atatcgatcc ctctagcagc
    28081 taaatcagtc gctacaacat attgatactc cagattctgt acttgtcgca tcactcgttt
    28141 tctctctctt ggggtgatgt ctccatgaat ctttgctact ttcaagcctt ggtttttcaa
    28201 gtaatcggca atttcatcca catgctgttt tgtattggca aatacgattg ctaaataagg
    28261 atgaccaatt gtcagcagtt gataaatcat ttgattctca tctttacctt ttgttgaaat
    28321 cagccaattt tcaatgtctt cagaaataac tgcttttggt ttgatttctt caatgattgg
    28381 attttccatg tattttcgta agaatggacg aagtttttct ggaatagtgg cagaaaaaac
    28441 taaaaactgc agcttttctg gtaatcgact tgcaatttgg tctacttcat ttaaaaaacc
    28501 catatccaat gtcatatccg cttcatcgac aacaaatgca aatgcggtgt gaatcttcag
    28561 cgcttgttca ttcatcatgt ctaatattct accaggcgtg cctactacaa cctgtggctg
    28621 ttggtgatgc agacgattta attgacgctg tttgtctgta ccgccgacaa aattcgagac
    28681 acggatctcc ggtgtagaaa atttagcgat ttgtgtagct gcttcataga tctgtgcagc
    28741 tagttcccta cttggagctg taataacaac ttgtacttcc tctttttctg cattgatttt
    28801 ttcaaacaaa ggcaacaaaa acgtatgtgt ttttcctgta ccagtttgag attgcccaat
    28861 gatatttttt ccttttgcaa ttaaaggaat caaacgttcc tggacctctg tgggctgttt
    28921 gaagcccttt gcggttaacg cttcattgat aaacggtttg aagcggaata tatcaaaatt
    28981 attcattcgt gcacctcttc tctttaaatt atcacgactt tggcattata acacagacaa
    29041 agcgtttttg ctcagctaat ttcgagaatc atcatccttt gtttggccaa tcgtgctaac
    29101 agtatatgtg tgactagctt tttcataaaa cagaatccgt ttttttctca aaaatgatag
    29161 caagatattt aaaataaaaa taccttgtag taacaagaca ccgagagaaa tgaatactgc
    29221 actcatctgc aacaccgtat tcgttgtcga attcatgatt tgctgaagca actgccccaa
    29281 ccagcttaaa ccataaaaaa gaaagtacag atacccgtag cggatcatca atgcttttaa
    29341 agaaattcgt tcgcggtctt cttccaccaa acggatacgc acaatttttt tgccaagtgt
    29401 ccatccattg gttataaacg gtacaatcat aaagtatacc cctacttcta ataaaagcca
    29461 ccagatatga gaagtaactg cccctgaatc gaacgcaatt ttaaagaaaa tcgccgctac
    29521 caaattaata agagataaaa atgtccagtc gatcaaataa gcgaccaagc gtcgagtcaa
    29581 tgagacagtc tttccttttt cataagaaag ttcatccatt cgtcttcttg taggaagcat
    29641 aaatgtaaat aacggcgtaa cgacaaaacc aatgattcca cccaatgtat ttgtcactaa
    29701 atcattcaca tcaaataagc ggtatggtct tgggtagata aaataaagtc cacttagctg
    29761 cgtcaattca aaaaatagag aaaggcagaa actagcaatc atcgttttca taaaagaaag
    29821 ccggaaataa tatcttaaat aaatgccaaa cggtatgagt aataaaacat taaaagctgg
    29881 ttccaaaaat acactctgct tcattgccgg caggtaggta ctaggttcag acaagaccaa
    29941 taccgtgttg tctctgaaat gttgccatga agcaaataaa tgcagctcca tctttggccc
    30001 tgtcatttgg gcaaccgtct cctttgccgg aagtggtaag atcaccaaaa aatacgcaca
    30061 caataaataa aaaacgaaag aatacaaaat aactgccctt gttactagaa atgttccata
    30121 ctttcgatat tcataaataa aaaaaacaga tgagatcgct aaggctaaaa atggaaaaac
    30181 aatcagcgca aaacgaatcg gttcgatata acttgccata tgactcctcc ttgattcttt
    30241 gtatctctct tatcttagca gtcctttgct gtttcaagca agtaacgagg ctattatttc
    30301 tcatttcttt caaagtaaag taagataaac acaaaggggg tgtcgagatg ctgaatatgt
    30361 actttgtttt tggcgtacct atttttttac tgttccttta tgctacgatc gcttatgtaa
    30421 gaaaaagaac tacaattcat tatttaggat ttattctcct gattatttcg ggtttcatgc
    30481 tagtcttcaa tcttcagact tggcagcaag cacttcttga aatggataaa atgactccac
    30541 atgctttgtc taaagtgctt ggctaccctg tctatctgat ttggcttccc attttcattt
    30601 ctggttgctt ggttttatta aatatttatc gcggcgtacg ccgaatagtt cagttacgca
    30661 aatcaaagta aaagacagaa atcggttgag cccgatttct gtcttttact ttgctatttc
    30721 atcgtagaca aaacgacatt tacgtgatca aaattaacga gtacttttga catttcttcc
    30781 acgtactcct catttaaata aaatattttg tttggcagag aagtgcagtt ctaataaaat
    30841 tcgaatgttt tagctatcta cgttttaaat agagataaat cgggatacca gcaagtgtca
    30901 atactatccc catcgaagct aaaaaggtct gagtgataag cgtgcttgat acgataaaag
    30961 tccctccaat gattgctact agtggaataa acggatacat tggaactttg tatggtcgaa
    31021 acaaatcagg ttctttttta cgaagaagaa tgacaccaac aaagaccatc gtataaaaaa
    31081 tccaaatgac aaagatcagc atatccgtta gtgtatcaaa accaccgatc atcatcatgc
    31141 caatagcgat gactagctct aagatacctg cggctacagg aactttagtt ttatcatgaa
    31201 gtttagcaaa gagcttgcta aaaggcaggt tgttttcttt tgccataaca taagggagac
    31261 gcattccagt aagggtatag ccattgatgg ttccataaac agaaatcaag ataccaactg
    31321 tgaccagtct tccaccgaag ccgccaaaaa tcatttttgc aacgtcactt gccgcattgc
    31381 tgtttcctgc gactccgtcg atagatgcgg ttcttaagaa caccgcattt actaataaat
    31441 aaacgatcat gattccgatg attcctaaag aaattgcttt tggcaaatct ttcgccggct
    31501 ttttcaattc tcccgaaata tttcctacgt gtatccaacc gtcgtatgcg aacatcgttg
    31561 ctagcaagcc cgctcccaag gctgagaaaa atgaaagatt ttctcctgct tggatcggaa
    31621 acagttggaa gtctactcct tcttgtcgga acaaaccgaa aatgacgatc acaaacaaag
    31681 gaataagctt gcagacaagc gtaatcgact gaaaagcccc tccagcttta gaacctaaaa
    31741 aattgatcaa caggatcgat acagcagctg taacagctac gggaacaatc attgactggg
    31801 aaagaccaaa tagattgacg aactgtgtac cgaaaatgat cgacaaggct gctacatttg
    31861 ctggaaaata aatcacgact tgcgcccagc ccaataaaaa agctgctgta tttccgtaaa
    31921 tccgttcgat atatttaatc atccctcctg tttctggaat agcagctgcc agttcagctc
    31981 ctgttagtcc tgcacagaca gaaatcatac caccgagaaa ccaagaaaac atatgcaaac
    32041 tggcggatcc tgtaacttct gctacgcttg cggctttaaa aaatacccct gcaccgatca
    32101 ctgtgcccat aacagtcgat aaagcagtaa aaaatcccat cgtacgtttt aattcatttt
    32161 ctatttttac ttcttttact ggcgtggctt gatattcttc catcccttgt cccctttcat
    32221 accatagcca atcccaaaag tataaaaaac tttttggatg tggttcttgt tttataccgt
    32281 ctctaccaat ttttctttca gtttttccaa catatccttt gtcatcacat ctaagtcgta
    32341 tttcggcgaa aagccccatt cctcttgcgc acaagaaaca tctagtgaat ttggccaaga
    32401 ttctgctatt gactgacgta caggatccac atcataatcc atttcaaaat ctggtatgat
    32461 tttttgaata gatgctttga tctcttccgg gtcaaaagac atcgccgata tattgaaggc
    32521 atttcgatga accaatcgat caggatctgc attcatgagt tttataatgg catcgatcgc
    32581 atctggcata tacatcatat ccattgatgt accttgatca atgaaactag tgtactttcc
    32641 ttctttgatt gctgaataat aaatatcgac tgcgtagtct gtcgtaccgc ctcctggcaa
    32701 ggttgcataa gaaatcaatc cagggaaacg gacaccacga gtatctacgc catatttcac
    32761 ataataataa tcacatagta gttctccagc taccttcgtt accccataca tggttgtagg
    32821 acgctgaaga gtatcttgtg gggtatgatc ttttggagta tttggaccaa acgcgccgat
    32881 tgaacttggc gtaaaaaatt tcagctggaa ttctctagcg acttccaatg cattgaccag
    32941 cccagtcacg ttcaaattcc aggctaattg tggttttgct tctgcaacag ctgacaaaag
    33001 agcagccaaa tgaatcaatg tgtccacttg atattgttct gctatgctgc gcattttttc
    33061 gtaatccatt acatctagga tctcaaagat atcgtttttc tcaatgactt caggcatgcg
    33121 tatatctgtc gcaatgacat tttcactgcc tagttcttga cgtagccgtg tgatcaactc
    33181 tgaaccaatt tgtcctaaac atcctgtcac caatactttt ttcatgatga tcctccctga
    33241 tcttttttac tactatcttc agatgattaa caatatatgc gtgaacatgt tctctaattt
    33301 taaatagacg aaaaaactaa atttagttaa ataatttcca tttcttttcc tattttttga
    33361 taaactgcta ctgcttcatc aagcatttcc ctcgtatgtt ccgcagtcgg catatttctg
    33421 atcctgcctg tcccaagagg cacagttgga taaacgattg gtttagcata aatcccatgt
    33481 tcgatcagct tcttagagaa agtctgagtc actttttcgt ctcccaaaat gactggagta
    33541 ataggtgttt cagacatacc gatgttatag cctacttttt tcaattcttc tttaaaataa
    33601 ttagcatttt cccaaacttt ttcgacaagt tccggatgtt cttgcatcaa agtaatcgaa
    33661 gccaatgcag ctgcagctgc acctggagtt agcgaagtag aaaataggaa tggccgactt
    33721 ctagctttca accagtcgat tagtgttttc gatccagcta cataaccccc aacaacacct
    33781 atagctttcg ataacgtgcc catttgcata tcgattttgt cgcttaatcc aaaatgcttg
    33841 acggttcctg caccttttcc agttacgcct gagccgtgcg catcatcgac gtaggtgatc
    33901 agtccatatt tttcaacaat cggaataatt tccggcaatc gtgcaatgtc tccatccatg
    33961 gagaaaacac catctgtgat atacatgatt tttttatatt tcttactttc tatcgcttcc
    34021 tttgcttttt tctcaaggtc tttcatatct tgatgtttaa ttcggatgat ttttgctccg
    34081 gataagcggc aaccgtcaat aatagatgca tggtttaatt catccgataa gatagcatct
    34141 tcttttgtca ttactgcaga gatagcgccc atattgcagt tgaatccaga ttgaaaggcg
    34201 attgctgctt ctgtcccttt gaattctgcg attttttctt ctaattgctg atggattttc
    34261 agcgaaccat tgatcgttcg gactgctcct gctcctacac cataagtttc ggttgcttca
    34321 atacatgctt ttttcaattc ttcgcgattg gcaaatccaa gataattgtt ggatgctaga
    34381 ttgatcatct tttttccgtc tacgatgata caagcaccat tttcactttc tagtatgtcg
    34441 atcgtattgt ataacccctt ttctttcagt tgatccaatt ctttctctaa atacattgtt
    34501 agttctttgg aagacattgt ctccacctcc atattcatct gtcttgcaga acataccaaa
    34561 catatatcgt gtcattcatc tacaaagcaa gtatgctata tttttgataa cgcttacact
    34621 gattatgaga attatttaac cacatttttt atttacagta tctttttact ttcaaatgac
    34681 ttatatagta tgaacaatta ccttttagat aatgatctat tttactctac ctgggcagat
    34741 aaatacaaca caaccattga tagaagccct ttcgttaaaa tcaattgtat tctgaatatt
    34801 taatttattt attgcataga ccaataaaaa tacataaaat ttccatcttc gtttatcagc
    34861 aatttttaat tttactctct gaatatttct taaaagtttt acttgttgaa ttcgaaaaaa
    34921 ggactgtgac aaaagtcttg tcacagtcct attcttccga ata
//
LOCUS       DAZKOU010000020        34448 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000020 DAZKOU010000000
VERSION     DAZKOU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.020"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG827_000944"
     CDS             352..1041
                     /locus_tag="ACG827_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0483104.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG827_000945"
     CDS             1028..2236
                     /locus_tag="ACG827_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0483105.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG827_000946"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG827_000946"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0483106.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG827_000947"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG827_000947"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0483107.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG827_000948"
     CDS             complement(6339..7034)
                     /locus_tag="ACG827_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0483108.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG827_000949"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG827_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0483109.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG827_000950"
     CDS             complement(8233..8424)
                     /locus_tag="ACG827_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0483110.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG827_000951"
     CDS             complement(8445..9335)
                     /locus_tag="ACG827_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0483111.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG827_000952"
     CDS             complement(9322..10089)
                     /locus_tag="ACG827_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0483112.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG827_000953"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG827_000953"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0483113.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG827_000954"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG827_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0483114.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG827_000955"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG827_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0483115.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG827_000956"
     CDS             14660..15043
                     /locus_tag="ACG827_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483116.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG827_000957"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG827_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0483117.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG827_000958"
     CDS             complement(15987..16805)
                     /locus_tag="ACG827_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0483118.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG827_000959"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG827_000959"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0483119.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG827_000960"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG827_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0483120.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG827_000961"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG827_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0483121.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG827_000962"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG827_000962"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0483122.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG827_000963"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG827_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0483123.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACG827_000964"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACG827_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0483124.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG827_000965"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG827_000965"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0483125.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG827_000966"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG827_000966"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0483126.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG827_000967"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG827_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0483127.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACG827_000968"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACG827_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0483128.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG827_000969"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG827_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0483129.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="ACG827_000970"
     CDS             28113..30086
                     /locus_tag="ACG827_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0483130.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="ACG827_000971"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="ACG827_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0483131.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG827_000972"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG827_000972"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0483132.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="ACG827_000973"
     CDS             32965..34257
                     /locus_tag="ACG827_000973"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0483133.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10510 a         6702 c         6821 g        10415 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25201 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25261 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26641 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34381 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc acagtccctt
    34441 tttccgaa
//
LOCUS       DAZKOU010000021        33479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000021 DAZKOU010000000
VERSION     DAZKOU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.021"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..291)
                     /locus_tag="ACG827_000974"
     CDS             complement(16..291)
                     /locus_tag="ACG827_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483134.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            585..1400
                     /gene="thiD"
                     /locus_tag="ACG827_000975"
     CDS             585..1400
                     /gene="thiD"
                     /locus_tag="ACG827_000975"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0483135.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1729..3180)
                     /gene="cls"
                     /locus_tag="ACG827_000976"
     CDS             complement(1729..3180)
                     /gene="cls"
                     /locus_tag="ACG827_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0483136.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3521..6187
                     /locus_tag="ACG827_000977"
     CDS             3521..6187
                     /locus_tag="ACG827_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0483137.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6551..7303
                     /locus_tag="ACG827_000978"
     CDS             6551..7303
                     /locus_tag="ACG827_000978"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0483138.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            7756..9144
                     /gene="ltrA"
                     /locus_tag="ACG827_000979"
     CDS             7756..9144
                     /gene="ltrA"
                     /locus_tag="ACG827_000979"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0483139.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(9266..10561)
                     /gene="purB"
                     /locus_tag="ACG827_000980"
     CDS             complement(9266..10561)
                     /gene="purB"
                     /locus_tag="ACG827_000980"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0483140.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(10574..11698)
                     /gene="purK"
                     /locus_tag="ACG827_000981"
     CDS             complement(10574..11698)
                     /gene="purK"
                     /locus_tag="ACG827_000981"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0483141.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(11691..12179)
                     /gene="purE"
                     /locus_tag="ACG827_000982"
     CDS             complement(11691..12179)
                     /gene="purE"
                     /locus_tag="ACG827_000982"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0483142.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(12595..13902)
                     /locus_tag="ACG827_000983"
     CDS             complement(12595..13902)
                     /locus_tag="ACG827_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0483143.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(13905..14486)
                     /locus_tag="ACG827_000984"
     CDS             complement(13905..14486)
                     /locus_tag="ACG827_000984"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0483144.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(14602..14699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(15058..16350)
                     /locus_tag="ACG827_000985"
     CDS             complement(15058..16350)
                     /locus_tag="ACG827_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0483145.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(16347..16970)
                     /locus_tag="ACG827_000986"
     CDS             complement(16347..16970)
                     /locus_tag="ACG827_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483146.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(16973..17809)
                     /locus_tag="ACG827_000987"
     CDS             complement(16973..17809)
                     /locus_tag="ACG827_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0483147.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(17826..18713)
                     /locus_tag="ACG827_000988"
     CDS             complement(17826..18713)
                     /locus_tag="ACG827_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0483148.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(18729..20012)
                     /locus_tag="ACG827_000989"
     CDS             complement(18729..20012)
                     /locus_tag="ACG827_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0483149.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(20695..21378)
                     /locus_tag="ACG827_000990"
     CDS             complement(20695..21378)
                     /locus_tag="ACG827_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0483150.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(21396..22013)
                     /locus_tag="ACG827_000991"
     CDS             complement(21396..22013)
                     /locus_tag="ACG827_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0483151.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(22023..22847)
                     /locus_tag="ACG827_000992"
     CDS             complement(22023..22847)
                     /locus_tag="ACG827_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0483152.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(22840..23400)
                     /locus_tag="ACG827_000993"
     CDS             complement(22840..23400)
                     /locus_tag="ACG827_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483153.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            23574..24530
                     /locus_tag="ACG827_000994"
     CDS             23574..24530
                     /locus_tag="ACG827_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0483154.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(24835..25791)
                     /locus_tag="ACG827_000995"
     CDS             complement(24835..25791)
                     /locus_tag="ACG827_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0483155.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            25895..26497
                     /locus_tag="ACG827_000996"
     CDS             25895..26497
                     /locus_tag="ACG827_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0483156.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(26585..28084)
                     /locus_tag="ACG827_000997"
     CDS             complement(26585..28084)
                     /locus_tag="ACG827_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0483157.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(28100..28624)
                     /locus_tag="ACG827_000998"
     CDS             complement(28100..28624)
                     /locus_tag="ACG827_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0483158.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            28922..29671
                     /locus_tag="ACG827_000999"
     CDS             28922..29671
                     /locus_tag="ACG827_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0483159.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            29662..30447
                     /locus_tag="ACG827_001000"
     CDS             29662..30447
                     /locus_tag="ACG827_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0483160.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            30416..31132
                     /locus_tag="ACG827_001001"
     CDS             30416..31132
                     /locus_tag="ACG827_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0483161.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(31234..32253)
                     /locus_tag="ACG827_001002"
     CDS             complement(31234..32253)
                     /locus_tag="ACG827_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0483162.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(32272..33273)
                     /locus_tag="ACG827_001003"
     CDS             complement(32272..33273)
                     /locus_tag="ACG827_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0483163.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT        10246 a         6828 c         6208 g        10197 t
ORIGIN      
        1 aagaacagtt agaagttaaa attgctgatt cacatagagt ttatgacttc ttggaaattc
       61 tttttcaatc agttttttca tctgtttatg aaattctttt ttttcagtaa acataaaggt
      121 ttgggtatcg taaccttctt catgtaggaa atagattatt cctttttctg tgttttgcgt
      181 ataagctttt atactcctgt cttttttgaa agaacagatc gtatagccat ctatttggtc
      241 tggtagtcgt ttgattttct tcaaaatgac ttttttatcc tcaagtttca tttttttcac
      301 ctgactttat ttttttcata tggaaatcat agcgcctttg gttatatgaa aacaagaagg
      361 atactgaaat atatataaat caaatagtgg ttatttatta ttagaatagc gaatacaaat
      421 aaacgaccac tattttctga tttattgcaa atgttttttc aaataaattt gattaccaag
      481 aaaaggtagt ggtatattta ctgagggata ctttgaaaaa catatgatag aaacagtaac
      541 agtattatga aaaatctttt tataaaaatg gaggaatcga tttgatggaa gatcagtatg
      601 tacaagcaat gacgatcgca ggatcagata gcgatggaag cgcagggatg caagcagata
      661 tgcatacatt ctttacaaga aatgtttacg gtgtttctgt catgaccgca tgtgttgcag
      721 gtaattctta cggaatcgga gcaagtgtga cattgccaac agattttatc gacaaagaat
      781 ttgaactgat tgcgaaggac tttcaaatac gtgcagctaa aacaggtatg ctagcagatt
      841 caaagttgat tgagacagtt gtaaaaaatt accaaaaata tgattttgga ccattagtcg
      901 tggatcccgt aatcgtaacg aaacatggaa atcttttact agaagaatct gctttgcaaa
      961 gcttgaaaga aaagcttgtc cctttggccg aagtactgac acctaatttt tatgaagcag
     1021 aaaagctgac tggacggaca ttagcagctg aagaagattt tgtgcaagca gcaaaagaac
     1081 tgcaagatat gggtgcaaaa aacgttatga tcaaaggaca tcatgctaaa aatgcgcagc
     1141 aagaaacagt caatgactac gtattgttcg aatcaggaaa atcattttgg ttaaatgcac
     1201 cattttatcc taccgaccgg atcaacggaa ctggcgattc gctatctgca tgtattgcag
     1261 ctgagttagc gaaaggaaaa acagttgaac aagcgatccg attggcaaaa gactatgtaa
     1321 accgtgcaat tcgtgaagaa ataaatgtag ggcacaaata tggccccatc aatcattggg
     1381 cagctggtga attagagtaa aagaaagaag ttgtgaaaaa gttgccctgc atcaagtaat
     1441 aagaacagcc aagtaaaaac agcttcttat gtttttaact tggttggact tattaccgca
     1501 gatgcaagct tttgaacacc gtttaagaaa gaggtcgtga gcctgccgtc cagctccgag
     1561 tcacaaggaa caagttcaaa aaacagcttc tcatgtttgt tgaatattgt gacttgtgac
     1621 cgaggagttg gcaggagaac accgtttgtt cggaagaagg gactgtgaca agatttttgt
     1681 cacagtccct ttaatcttag tatatttttc tctacttgaa taaaaaaatt ataggattgg
     1741 agaaaataat cgagaaattt gttgcttgaa taatagccag ttagacattt ccttgactgt
     1801 ttcagttgtc atggatgaac aatgtgtctc atcttctttg aactgagtag caagttcttc
     1861 taaaaactca ggatcgtaaa tgacagcact tgtttcaaaa ttcaatcggt agctacgaat
     1921 atcttgattt gctgaaccta ccatgcagat ttcatcatcc attatcaatg tttttgcatg
     1981 gaggaagccg ttctcataga caaggatttg tacattttcc ttgatcagct gccgagcata
     2041 atattgtgtt gctcgataaa taaaaggatg atccggtttg ttgggaatca tgattttcac
     2101 atctacacca gaggcagttg cgatttttaa agcagcaatg acactttcat caggaacaag
     2161 gtaaggtgtc tgtatccaaa cacgtttttt agcagaagta atcaatttga taaatgaaag
     2221 cttgatttgt tccctttcgt tattcggtcc gcttgaaaca agctggatgg atgtatcagc
     2281 aagcttagat tcatctcgat ctgctttttt aaagaaataa ttcaaatgat agccgacctt
     2341 tttttcttca ggtaccgaga cgttccagtc tgttaagaag cgaagctgga gcaaagaaga
     2401 agcggcaccg aaaatccgta tatgcgtatc gcgccaatag ccgaattttt ttgttgtatt
     2461 tacatattga ttggcaatat tgaaaccacc ggtataacta atctttccat cgatcacaac
     2521 gatttttcgg tgatcatggt aattcaatcg gaaacgaagt aatgcccttt gtgaggtaac
     2581 aaatgtatgg acatgtccac catttttgat tagacgattg aaatcttttg cttttgtgcc
     2641 atgagagcca aatgcatcat ataatattcg aacttccacg ccttctgcgg ctttttcttc
     2701 taaaacatgt aagacttgct ggccgatatt atctgtcaca aatgcgtaat attcgatatg
     2761 gatcgaatgt ttggcttttt ttagatcttg aagcagtgaa tccaatttct cttgtccgtc
     2821 tgtgagaaga gtgacagaat tcattcttgt cagcggcata cgatttaatg aagagaagaa
     2881 gtcaacaaat tgctgtttgt ctttatcgcc catgtcagga tcgtaatgtt caatggtatc
     2941 tcctctaaaa gactgaaagt tttctaattc tttcaagtca ctttgtcgga gatagaattt
     3001 tttcttatcc gttaatccac gtccgaaaaa taaatataat acaaagccca ccccaggaag
     3061 ggcaaacaat actaagagcc atgcccaaat ggctgctaca tctcggggtt tgatcaaaat
     3121 agccaccaaa gcaataagtg catttaatag ataaagggcg gttataatac tagataccac
     3181 aaaaatgttc ctccttacat acaataacat tagtatagca tgatagaaga aagaagccta
     3241 ccaaggtgaa tatacttttc actcctttca tgaaaatgta ggtaaatcgg gctaaagtag
     3301 ttgctttttg taggttaata aggtaggata ttagtacagt tgaatatctc cttaggggag
     3361 taaccggcaa cgtttgttgc gataatatca acagtaagtt aaccaaatgg ttttctggta
     3421 ttgtatgaaa tggtgagacc taagacaaat gcgtatttgt cttaggtctc ttttttttca
     3481 gacaggacta gggccaagat caaaaaatgg gggatgaaat ttggaaaaca aggaacaagc
     3541 acaacaagcg gtctataaag aaacgatcga ctcgttgatg cagcaaacgc agagctcaac
     3601 cgatggattg agcaccacgc aagcgaagga aaaattagaa agagatggct tgaaccagct
     3661 aaaagaaaag ccgaagaagc caacttggaa attgttctta gaaactttca aagatgcaat
     3721 ggttatcgta ttgctgattg tcgcactgat tcagatggtc atgggtgact acatcgaatt
     3781 cttagttatt tttgcagtat tgatgttgaa ttctataatc agtgttgtcc aaacaaaaaa
     3841 agcagaaggt tctttagaag cgttgaaaaa cctttcagcg cccgatgctc gagtgattcg
     3901 tgatggtaag gaaaaaacga ttccggcaaa cgaacttgtg gtaggagata tcgtgttgtt
     3961 agacgcaggc gattatgtac ctgctgatgg acgtttacta gatgcgggat cattaaaagt
     4021 agatgaaggt atgctgacag gagagtcgac ttctgcagaa aaagaagtaa cggatatcac
     4081 aagtacggta ccaatcggag atcgtacaaa tatggtattc agtggaacga tcgtaactta
     4141 cggacgcggt aaattcttag tgacttcaac tgggaataat acggagatcg gacaagttgc
     4201 tggccttttg gaatcaacaa ctgagcaagt cactccgtta caacgaggtc tggataaatt
     4261 cagtaaaaaa ctaagtttgg ctattttggc attgtcttta ttgattttag gtatccaact
     4321 gtttcgtatt tatcttggag aaggtacagg agatatgact gcagatatcg tcagcgcagt
     4381 gatgtttgcc gtagccgtag ccgtagctgc tattccagaa gcattacagt ctatcgtaac
     4441 aatcgtgtta tctttgggaa cgaataaaat ggcaaaacgt catgccatta ttcggaagct
     4501 tccagcagtt gaaacacttg gttcgacaag tatcatctgt acagataaga ctggtacatt
     4561 gacacaaaac aaaatgacag ttgttgacta ctttttaggt aatggcgata ctggggattt
     4621 cacaaatgac ccttcaaaat ggacagctga cgaacgtcgc ttgatcgaaa tcagtgtttt
     4681 agcgaatgat gcatcgatca gtgaagacgg tactaaattg ggcgacccta cggaagtagc
     4741 gtttatcgac tttagtgaaa aattaaacca gccttatcag gaaatccgaa acaactaccc
     4801 aagacaagca gaacttcctt ttgactcaga tcgaaaatta atgtctactg gacacacaat
     4861 tgataacaaa aattacttgt ttgttaaagg cggaccagat atcgtcttcc aacgcagtaa
     4921 aaaagtattg ctaaatggtg aagtcgttcc gatgacggat gaattgatga aaaaattcca
     4981 agagcaaaat gaagcttttt caacacgcgc attacgtgta ctagcttttg cttaccgtcc
     5041 acttgataat tttgatttat catttgaaga tgaagacgat ttgatccttg ttggtttgct
     5101 tgctatgatc gatcctccac gtgctgaagt gactcaggcg gtaacagaag caagaagtgc
     5161 aggaatcaaa acaatcatga ttactggtga ccacaaaaca acagctgttg ctatcgcaaa
     5221 acaaatcggg atttttgaag aaggaaacct ggctttgacc gggacggaac tagatgaatt
     5281 gacagaagat caactaaacg aagttttaga aaaagtcact gtttatgcac gtgtttcacc
     5341 tgagaataaa attcgtatcg ttcgtgcttg gcaaaataaa ggtcatatct cagctatgac
     5401 aggtgatggt gtaaatgacg caccagcatt gaaacaagca gacatcggaa ttgctatggg
     5461 aacgggtaca gatgtggcaa aagatgcagc agcgatggta ttgacggatg ataactttgc
     5521 ttctattgtc agcgcagtag aagtaggacg taatgtgttt gataatatca agaaagctat
     5581 ttcttatcta tttgcaggaa atttaggtgc gatcattgcg atcatctttg ccttagttgc
     5641 tgactggtcc aatccattta cagcattaca actgttgttc atcaatttag tcaatgattc
     5701 actgccagca attgcattag gtacagaaaa agctgaacct gctacgatga accgcccgcc
     5761 aagagatcca aattcaggaa tctttacgga caaaacattg atttctgtaa cgtatcgtgg
     5821 tgtgctgatt gggattgctg ttatctttgc tcaatggctt ggcatgcagc attcacctga
     5881 attcggtgta gccatgtcat tttcaacatt gatttggtgt cggacattgc aaacattccc
     5941 agcacgttca aacacacaaa cagctatcca agcagggttc ttctctaaca gaagtgtctt
     6001 actagctgta gttgtatgta gcgcgcttta ctgcttaaca atgatcccag gactgaaaga
     6061 agtcttctct atcccatcag catttggatt gacagaagtc tttacatgta tgggaatcgc
     6121 acttgtgaca attgttttga tggaaatcac taaattgatc attgtacgag tacaaggaaa
     6181 taaataaaac gaatcaacga ggttgtgagc ctgccgttta attctgagtc acaaggtgca
     6241 atttcaacaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aagagttggc
     6301 aggagaacac cgtttaagga agaagttgta aaatcagcat tttgagctaa tactgagggt
     6361 catctgattg gacactgttt attcggaaag agggactttg acacgacttt ggtcacagtc
     6421 ccttttttta tttatgatag acggaaagcc aatcatttta ttattcatca aaagagatag
     6481 aatagataaa agaggagaat gatttattcc gaaaagtgaa aacatataac aaaaaattag
     6541 aggaaatcaa atgaatgaaa aagaatggga tgaatttgct gaagaatatt atgaaatcca
     6601 gcaagaatcg caagtaacta ttgctgaaga tgtaaaaaat tatctgaaaa cggagaaagt
     6661 cctgcctgct gaaacagcag cagacgttgc tggaggatct ggcagatttc tttccttagc
     6721 aaaagaaatt aatcgctatg atttgatcga tttttcttca ggaatgctag aaaatgcaaa
     6781 aaaagaagca gaaaaaaatg agttgccaaa tgtttccttt atcaaacagt cgttagaaga
     6841 atttatccaa tcaaatgaga catatgaaat ggtttttaca gcagcaaatc cagcattggt
     6901 acatcttatt caattagaaa aattgatgaa aaagtcaagc aaatggtgcg tgataatcag
     6961 agtagtggaa tcaatcgatg atgtttttta tcctatcgaa aagcaactag gaatgataga
     7021 agaagatcct aatacatcgc cagttgtaat ggatcaattt gaaacatact taattgatga
     7081 aggattcaaa gtgaagaaaa aagattttac ttattttaca caggaatcaa ttactgaaga
     7141 attgatgaag ctatactatg acgcgtacgc aaaagatcct gttgtgacca ggtacattcg
     7201 caaactattc agtgaaaaac gagaaatcct tagtacgact aagctgacct atagactgct
     7261 cctttggtcc gcagataaaa aaagaacctt tcacgactcg tgaagggttc tttttatgat
     7321 gtgcgccctg catgggtgaa aacttggtgg tgaaagtcca ctacaggctt ggcagtagga
     7381 actgttagcg aaagacaagg cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa
     7441 actcctgaac tgacgaacag aaactagata caaggctgaa ttaggtcgga taagtcttca
     7501 tcacaagaca aagtccaata ctgcccgaaa cctatacagt aaatctagca gttacatggg
     7561 aggaaagttt tcactcttac cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa
     7621 aacaaaccag cacaacgatc atggcgacat gatggtgaat catgagaagt cagccgaggt
     7681 catagtagta acgacactga ccgttacgaa ggactgaaca ataagaatct tgaataattt
     7741 aggaggtgtg agaagatgcg acgaccgcag aaaacaagaa aacttggcta tcatcaaaga
     7801 gctagtgtgg aacacgaagg gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg
     7861 aaacaagatg gtgtagaaaa tctgtttggg agaattctag atcggaataa tctcaatcaa
     7921 gcgtatcttc aagtagtcag aaataaaggg gcggctggtg cagatgggat gaccgtcgat
     7981 caactgcttc cttacttgaa agagcaccgc gaggaattac tatccaattt acgccaaggg
     8041 aactataaac ctcagcctgt tttgcgagta gaaataccga aaccaaacgg cggtgtaaga
     8101 aaactaggaa ttcccacggt tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta
     8161 caaccgctat ttgaaccaga attctcagat aatagttatg ggtttcgccc aaatcgcagc
     8221 gcacatgacg cagtgaagaa agcaaaatct tattatgaac aaggttaccg atatgtggtg
     8281 gacattgata tgaaagccta ctttgacacc gtaaatcacg ataagctgat gtatttcatt
     8341 gagcggaaag taaaggacaa acaagtccta aaactgatcc gtcaatactt aatgagtggt
     8401 gtaatggaaa atggactcat cacaacaact gaagaaggaa cgccccaagg aggcaatctc
     8461 tctcccctgt taagtaacat ctacctgaac gaatttgatc aagtgctaga aaatctagga
     8521 cataaatttg ttcgatacgc cgatgactgc aatatctatg ttagaagcaa acgagcagga
     8581 cagcgtgtga tgaagaaatc cattcaattt ctcgaagaac aactaaagtt gacggtaaat
     8641 cgagaaaaga gtgcggtagg aagtccattg aaacgaaagt ttcttggatt ctgcattctg
     8701 ccaacaaaga aaggtattaa aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc
     8761 aagctcaaac aactcactaa aagaaaccgt ggaagaagta tccaacttat tttaaaagaa
     8821 atcaagcaac taatgactgg ttggatcaac tactatggaa ttggtgagat gaaagaattc
     8881 atgagggagt taaacggttg gttgaagcga agaatcagac aatatatttg gaaacaatgg
     8941 aagaatccaa gaaccaaaag aaaaaatctg attcgtttag ggatcgataa agcgaaagcg
     9001 tatgaatggt caaatacaag aaaaggtatt tggtcagtct ccaaaagtca tattcttcat
     9061 cgatcattaa cagacaaaga actggcccgc caaggatatg aggacatctc cttgaagtac
     9121 cagttcgtac actcaactta ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga
     9181 gaggggcgga aggtcagttg gctttcccct ctactcgatt atttttgcta ttctatttta
     9241 attgaatttt gcaactcact cttctttatt ccaaacctac tcgtttgaat atcacgtcaa
     9301 cgtttttcaa atgatgatga taatcaaagg catcgtctat ttcttcttta gataataccg
     9361 atgtgatggt tggatcttct tctaacagcg ggcggaactg cacttggttg tcccaagcgt
     9421 acgcagtttt tggctgaaca agatcataag cttcttctct tgtcattccc ttatcgatca
     9481 atttcagcat aacgcgctgg ctataaatca aaccaaatgt tgcagacata ttccgcttca
     9541 tgttttcagg aaagaccgtt agatttttaa cgatattgcc aaaacgattt aacatgtaat
     9601 tcaataaaat cgtggtatca ggcaagatga ttcgttctgc tgaagaatga gaaatatctc
     9661 tttcatgcca taatgtgaca ttttcatagg cagtgaccat gtgtccgcgc accactctgg
     9721 ctagaccagc catattttct gaaccgatcg gattcctttt gtgcggcata gcagaagaac
     9781 ctttttgacc tttcgcaaaa aattcttcta cttcacgtgt ttcggatttt tgcaatccgc
     9841 gtatctctgt tgcaaaacgt tcaatacttg tagcaataag cgccatcgct gagaaatatt
     9901 cagcatgtag atcccgaggg agaacttgag tagagatttc ttgtggacgg atccccaatt
     9961 gtttacatac atactcttca ataaatggcg ggatatttgc gaacgttccc actgctcctg
    10021 agatttttcc tgcttctact ccttttgccg catgttcaaa tcgttcgatg ttccgtttca
    10081 tttctgaata ccatgtggca agtttcaatc caaaagtagt tggttcagca tggacaccgt
    10141 gtgtccgacc catcatcact gtatatttgt gttcttttgc tttttcgcca atgatttccg
    10201 taaaacgttt caagtcttcc cgtaaaatat catttgcttg cttgatgaga tagccataag
    10261 cagtatctac gacatccgta cttgttagac cataatggac ccattttctt tcttctccca
    10321 atgtttcaga aaccgctcgt gtaaacgcaa cgacatcatg gcgtgttaat ttttcgattt
    10381 ccaagatccg atcgatatca aatgatgcat gctcacgaat ttttttcaca tcttcttgcg
    10441 ggatttcacc aagttctgcc caagcttcat ctgctaaaat ctcgacttcg agccacgcac
    10501 ggtatttgtt ctcttccgtc cagatattcc ccatttcagg gcgtgtataa cgttctaaca
    10561 tgttttttct cctctaatcc caaatattcg tttgtttgat ttcttccagc gtttgctgga
    10621 tgtcttctgt caagatagtg atatgtccca tcttgcgacc tttttttgct tgtttttttc
    10681 catagaaatg gaattgccag tctggcttat tctcaatcaa gcgatagctt tccagcagtt
    10741 gttctcccaa aacattgacc attactgcat tgcttatcaa acgtactttt tctcctaatg
    10801 gccaaccaca aatacctcga atatgggcat cgaactgact catagaacaa gcttcaatag
    10861 aataatgtcc agaattgtga ggacgaggag ctaattcatt cacatatagc ccgcctgttt
    10921 ttgttaaaaa catttctacc cctaaaacac ctgaaaggct cactgcttct gcaatcacac
    10981 gggcaatacg ctcagcctcc tcgatcacat cctgatcaat cgcggctgga gcaatcgttt
    11041 catgaagaat attgttgtgg tggatgtttt ccacgaccgg aaaagtcgtg aaatctcctt
    11101 gaccatttcc tgccaccatg atcgaaatct ctttttcaaa cgggatccat gcttctaaaa
    11161 cacaggttcc ttctcttaaa aggtccatgg caggagctag atccgcccga cttttcaaaa
    11221 catactgccc cttgccatca tagccgcctc gagtggtttt caaaacacaa ggatagccga
    11281 tcccgtcaat cgcatcttgg atatccgtcg gactgacgat cgttgcataa ggagcaatca
    11341 cgatgttatt cgtttccaaa aaagattttt ctaacaggcg gtcttgcgta atcgcaagca
    11401 agtctgtccc ttgaggaata aatgacatgg gtaaaatcgc atttaacgcc tctacactga
    11461 cgttttcgaa ttcataggta atgacatctg ttcttctggc aagctctttc agcgcaaagg
    11521 tgtcatcata gacactttca atgtgccaat ctgcaacttg tgcggctgga caatcgctca
    11581 caggatccag cacacctact ttaaagccca tttcttttgc actaatcgcc atcatacgac
    11641 ctaattgacc gccaccgacg atcccgatcg ttgaaccagg caacaaaggt ttaaccaagt
    11701 tgatcactac tttctataac agttttagcc aacattgaac gtctttcagc caatttagcc
    11761 tccaattcta aatcatacat ggaaagcatc tgtatcgcaa gtagtccagc attaattgct
    11821 cccgcttttc cgattgctgt agtagctaca ggtactcctc caggcatttg gacaatcgaa
    11881 agaagcgaat ccaaaccatt taatgttcgt gattggaccg gtacaccgat aaccggcaaa
    11941 gttgtttttg atgcgaccat acccggcaga tgcgcggctc cccctgctcc tgcaataatg
    12001 attttgattc ctttttttcg agcgttttgt gcatattcaa acatcaaatc tggggttcga
    12061 tgagcagaaa cgaccttttt ttcataagac acaccgaatt catccaatat ttgacatgct
    12121 tctttcattg tttcccaatc tgaagtactc cccataatca cagagatttt tgctgtcatt
    12181 gtttttcctc ccagtttttc tacattcata ttttaacaat cacttttttt gatgtcaaag
    12241 aaatttcgaa tgttaacaaa gaaaaacatt ttattgttcg tgtttattaa agataaaaaa
    12301 aaggaatgtg acaaaagtta ggtcacattc ctttttatgg ataaacggtg ttctcctgcc
    12361 aactcctcgg tcacaaggaa caatattcaa caaacatgag aagctttttt ttgaaattgt
    12421 tccttgtgac tcggagatga acggcaggct cacgacctct tccttcaacg gtgttcaatc
    12481 agctgaccct cggtattagc tcaaacaaac ggcaaaatac gaggaactat ttttccgttt
    12541 gtttctctaa caatcaggtc aaaacgctga tttcacaacc tttcttttgc tctattactt
    12601 cttgaaggct tcttccaata tttcttcatc ttttttcaat ccattaaaga tcaaattcaa
    12661 tatgatagcc gtaatgctac tcattacgat gccatttcca gtgaacattt gcaatgtttg
    12721 cggcatttga ctgaataatg tcggcgtaac attgaaaccc agcccaaatc caacagaaac
    12781 agcaacaatc aatagatttt tatcattgct gaaatcaact tccaacagca tacggattcc
    12841 ttggactgcc accatcccga acatgactaa cattcctcca ccaagtaccg gctcagggat
    12901 gatttgtgct aacgcaccga ttttagggaa taatcctaaa acaatcaaga agaatgcaga
    12961 gaaatagatg ggttttcttg ttttgattcc cgaaagttga accaatccga cattttgaga
    13021 aaagccagta taagggaatg tattgaaaat accgcctaag ataacggcaa gtccttctgc
    13081 acgatatccc tttttcaatt cttcttctcc aacatttttc ccagtaatgt caccaagtgc
    13141 aaaatataca cctgttgatt ctaccatgct gaccatggaa atgatgatca ttagtaaaat
    13201 cgaccatacg tcgaaagttg gtttaccaaa ataaaagggt tgtggaacat ggaacaacgg
    13261 cgcatctcca atcgcgctca gatcaatcat tcccatgaaa gcagctaaga tgcttcctcc
    13321 gactaaacca atcaataccg cgattgaacg aatgaaccct tttgccagca tttgcacgcc
    13381 tacaattagg agaatcgtca caaatgcaag tagcagattt gttttgtcac caaaatcagc
    13441 tgctgttgca ttacctccac ccattttctc gattgctaca ggaatcaaag tcaaaccaat
    13501 aacggtgata accgttcctg ttactatagg agggaatagt tttttaattt tagaaaagac
    13561 accagaaact aacacaacaa agatcccaga agcgataata gatccgtaga tagctcctac
    13621 tccttcattg gttccgatca agatcagtgg agctactgct tggatcgcac atcctaaaac
    13681 gacaggcaag ccaattccga aaaatcggtt aactgtcaat tgcaataatg tcgctaatcc
    13741 acacataaag atatcaattg aaatcaaata agtcatctgt tcttgattaa agcctagtcc
    13801 tgtaccgatc aaaagaggca ccgctactgc tcctgcatac atcgctagca gatgctgcag
    13861 ccctaaaacc gcagcttttc cattttgtgt ttctttttcc acgattatgc gtcctcctca
    13921 agaaactcaa cttgtccatt cgtcaaggaa gcaatacgcg caagagaaac gactctcaat
    13981 cccatttctt caagtaactg acgtccatct tggaaagact tttcgatgac gatcccgatt
    14041 ccttctactt gcgctccagc ttgctggcat aattcgatca atccttttgc cgcttgtccg
    14101 tttgccaaga aatcgtcgat aatcaatact ttgtcttctg aagataaaaa tttacgagaa
    14161 atagaaatcg tacttgtcac ttgtttcgta aaagaataaa cagaagctgt caataattct
    14221 tcatccatcg tcaaactttt tgcttttcta gcgaagatca ttggaacatc taattctctt
    14281 gcagcaaata aagctggtgc gatcccagaa gcttcaatgg tcacaacttt cgtgattcct
    14341 tgttctttaa atacttctgc aaaccgtttt cccatttgtt ccatcaatac agggtctact
    14401 tggtgagtga cgaagctgtc gacttttaat acgccctcgc ctaatatacg tccgtctttt
    14461 tgaatacgtt caactaattc tttcacttta tttgctcctt tatcatttat taataccgaa
    14521 ataaccgctt ttgtgcaaaa acctactatg tttctgttaa agaaaaacac cctccacttt
    14581 atcataagaa aagaagggtg aaatagccaa tcttttactt atagtccagc atttacggta
    14641 ctggctagaa actgtcatcc atattatgac gatatataag taggtatgta aaactaataa
    14701 tagtgtaaat tcattttctt gcaactgttt tgcacgtgaa ggcagtttgc catatttgaa
    14761 taaaaaatgt tgtttcaaat attgaaaaca gtctaacact actttctgaa actgacaacg
    14821 tggtttactt atttttttgt aaaatctaat gatttttaat gcattattcc tttttgttcg
    14881 ggtttaaata gttattttta ttttctccta tctaaaaaag cagcccacat aaaaagaagg
    14941 catgacaaaa agcatgtcat agcccctttt tccgaataaa tagtgttcaa aagacagcct
    15001 atcggcaata aggcaagatt ttataaacta cgggaagtaa tttccgtaaa tctattctta
    15061 ttcttttaag ctgacaagct cgtcaatcac tactgctttt cctgaagcaa tagaatgatt
    15121 tgctgcgatt cctgtcaaaa tcgacaaaat accatctacg tgagttgctg cccgattcca
    15181 cggatcttct tctttttctt caccaaatag atcatctaat aaacgcggat ctcctccgcc
    15241 atgtccgccc gcttcttgtt cgacgattac ctcatatggc tcaccgaaca tcggatagac
    15301 atggatggac tgaagaacgg ctgctccttc atccttttta tctccacctg cattgatgta
    15361 agacttttcg atcactttca tttcgatccg tcctttgctg ccattgaaag aaacattgaa
    15421 tccttcccac ggcatatacg cgtttagcga atacgtcaaa atcgcaccag tttcatattt
    15481 gaccgttacg cctagtgtat cttcaataga gatatcatcg gaaaatacac tgcggtcacg
    15541 aatatatcca ctgtcttttt ctgcttctaa atacatttct cgcaattcct cattggctga
    15601 caggtctata gcaaatggat cagctttagc tagttcgctt ccatagcatc gactgtaaaa
    15661 tatttctact ccgcgtttct ccgcattatg catcccataa aagtttaatc caccgaatgc
    15721 aaataccttt tggggacggc tgttcaacca aaaattcact agatcaaaat gatgggtaga
    15781 tttatgaaca agaagcccgc cgctattgtg cttatttcga tgccatcttc tgaaataatc
    15841 tgccccgtgt tgcgtattta acagccattc gaagtggata gaaaacacat caccaatcac
    15901 atcactttct atcagttcct ttatcttcgt gtgatacggt gcataacgat aattaaaagt
    15961 gactcgcagg cttcttcccg tctgtttttg gaccgttatt atttcttgag ctttttcctc
    16021 atcgatagtc agaggttttt cgcaaataac atcatatcct tttttcattg cacgaatgat
    16081 ataatcatga tgcgttcgat ctactgacgt aataatgact tcagccggcc gtacgtcatc
    16141 cagtagttgc tcaaattgtg atggcataca tgtctttact tttttctgtc catacttgtt
    16201 cacaagtatc cgatttgcat aatccatcct tgtttgagat acatcacaaa aaccgactag
    16261 ttcacaagtt tgatgatacg tagaggcaat tgcctcataa aaaaaacgcg ctctcccacc
    16321 aagtccgact tgtacatatt ttttcttcat ttaaagtcat tctccttttc ttttaagcaa
    16381 aatactgtga caaatggcag ccctggatac agtaatgcag tgagtaaaaa atcatgctgt
    16441 atacaccaga acagtccata caaaaggcat aaataccaca gaaacccctt gacgtttttt
    16501 gacaacagcc agatattcct tttgatcaaa taaacaagac tatctgcttt atcttctttg
    16561 cagatatcaa ttagcgtact aaaaaaacta tgggatacaa aaagcaacag acacaataga
    16621 aaaggaatca gataccaagc tccaatcttt cccactaata aataccaatc aactgcaaaa
    16681 ataatgacaa acagctgaaa caacgtaatg atgaaacaca tccgtttgat ttttcctttc
    16741 ctccaacata cctttttttc tgccaagtaa tggacagata gataataaga aggatacatc
    16801 atcaaaaaat aaatcagata gacaacacga gcagacagca ctaatcctaa agtgaacaaa
    16861 catcctagaa ctaaagcgaa aagagcatta ctgataagac tggttaaaaa aatccgacag
    16921 acatagtagt tgagcttgta aagaaacatc tcttctattg gctttttcat attcagccct
    16981 tcagaccgct tgtactgatc ccctggacaa gatatttatt gaagaataag aaaatcagaa
    17041 aaaccggtag cagcgacaac gtagacatcg caaacatact cgcgtaagtc gtactagatc
    17101 cagcgtctgc aaataatttg atggcaatcg ataccgtgta agatctcggc ttgtttaaat
    17161 aaatcaatgg tcccatgaag tcgtcccatt tccaataaaa ctggatgatg atcgtcgtga
    17221 tgatcgaagg tttcagcaaa ggaaaaataa ttttggtaaa gacactatac ttgctgcatc
    17281 cgtcaataat cgctgattca tctaactctt tggggatgct gaccatgaat tgcatgatct
    17341 ggtaaatgaa aaatgcttgt ccaaaaaaat gtggtaaaat cagcggcaca tacgtcccaa
    17401 ccaaattcag ccgattgtaa ataataaact gcggaatcaa aatcacttgt gcaggaatca
    17461 tcatcgttac taacatgacg acgaaccaga atctttttcc tttgaaatcg attctcgata
    17521 atgcgtaagc aatacaagtc gagctcagaa ctgtccctat cgtcgaaact gcagagatga
    17581 caaacgaatt cctaaagaaa gtaaaaaatg aaattccagc aaaccccgcc catccttttg
    17641 cgtaattttc ccattgaaaa gtctctggaa tcaaattcag cgaaccactc ataatctctt
    17701 gatccgtttt aaaagaacct gcgatcatcc ataagaccgg ataaatcatg actagaccta
    17761 atagcaaaat aaaaccgtat tgaaaccatt tcgttactgt attattcatt tcttcctcct
    17821 ctcattcaca caacgaccca tttttttgat gatttgaaaa tcagaaaggt gacagctccc
    17881 ataatcaaca gcatgatcca cgaaatagca ctagcatacc ccatagaatt aaaagaaaaa
    17941 gcttggttat acacataaag agcataaaag ttcgtggcat cattcggacc accttgggta
    18001 atgataaatg cctgcgtaaa agtcataaac gccgcgattg tctgcataac caagttatac
    18061 aaaatgatcg gcgaaagaag aggtaacgtg atcttgtaaa acgtctgcca tttgttcgct
    18121 ccatcgatct ctgccgcttc ataatacgag cctgggatct ctttgatacc tgcagcaaag
    18181 atgatcattg ccgaaccgaa ttgccaagca gacatcaaaa caagagggat catgaccagt
    18241 ttttgatcgc taaaccagga aagcgcaggt gcgcctaatc gttccaggaa aatattcact
    18301 aacccgtttt tagaaaacaa ctcccgccaa actagagcga tagaaatact gccaccgatc
    18361 aaactaggca cataatacaa agaacgaaag aaagtcgttc ctttgatatt cctcgttaaa
    18421 agataagcaa tcaatagacc aaagatcagt ttcacaggta cagatgcaaa tacatagaga
    18481 aaagtgactc ggatggagtt caaaaaacgg gcatcactca gcatctttat ataattatcc
    18541 aagccgatcc atgagactgg atttcccaaa cgataatcgg tgaacgaata ataaagtgac
    18601 aagatcatcg gtatcactgt tatcaataga aatcctagga tgaatggaga agcaaatacg
    18661 taaccgacca cattgtcttg atccaagaac cgttttagtt tttctttttt catgccttcc
    18721 tcctgctgct atcctagaat gtttgacgca aactcataaa gactctctgc tgcttcttct
    18781 gctgttactt cttcataaat gactttttca actaaaagat tgtattggtc ttgaatttcc
    18841 ggattcaaag cactatcaaa gacattcact tcgccgttgt ccatatcgga aatcatttct
    18901 aaataatcaa atgtcgcttg caaagttgca tcatctgcac gagcgagctg ttctcgaata
    18961 ttcgacatga ttggtacacc acgttctcct tttaaaattt cattggcttc ttcactgttt
    19021 tgaaagaaat caataaactt agcagcttct tctttatggg ctgagctttt tgccatactc
    19081 agcatttgcg aagaacgtaa agggatacca gattctccat ctgcttttct gcgaggtgga
    19141 ttaatcaact gaatctccat atcttcagga gcccctgaca atatttctgt caactggtta
    19201 cttgatagaa acaccattgc cgcttctcta tccctaaccg cctgaaaaga cagatccgtc
    19261 gtacttgcag ctccaccacc tggttctgga tgggcccctt tgtctaccag atttttacgc
    19321 agctggaaaa aatctgttag atagtctgga ttttcaaatc ccaagctgtt agatagatct
    19381 tcccgattat aaaaattcaa tcctttctca acttgtggta tttgaataat acagccagct
    19441 ataaaatctt cgaatcttga aatagcatag attcctaatt cttcagtgat ttgatagctg
    19501 atttgttcaa attcatccca tgtccaattt gaagctggtt cagggatatt taattgttca
    19561 aatatatttt tattgtaagc aatcgcaaac gaattcaccc cgttagacag tccaactaat
    19621 tgcccgtcgt aagtcgtcgt tgctaaatag gattcatctg cattagaaat atcaattatc
    19681 ccttgctcca catattcatc taatggttca atctgattga tgtattgagg aaattgattg
    19741 ttcagttgga aaatgtccca gatatcatct gatgctgcta gtgtacctaa ttttgtgtaa
    19801 tatccttcat aatcataaaa ttcataatcg atcgtcacat ggggattttt ttcttcataa
    19861 agctcgatta cttttactgt tgcatcgtgt cggacttgcg aaccccacca agccatgttc
    19921 agcgtgattt ttttgtcatc ttcttgccca caagccgtca agaagaataa cataacactt
    19981 aaagctaaga tgattctcat tcttttcttc attctctgca cctctacttc cattgttatt
    20041 tttcaatata ctgtattttt atacagaaca tatttattaa acgatctttt tcaaggggaa
    20101 taaaaaccaa gacttgcctt atcgttctgc ctaggatgaa caactcaata ctttcaagaa
    20161 aaattagata gtgctgattc ttctttcatc gctaaaaaca cttcttgctt caatgatggg
    20221 aggataatta cgcaaagaaa aaaggatatc agacaactgc ccaatatcct tgacatagct
    20281 aaataagcat gcttcttcgt gtgcgctctt ccctaaaaaa cagaaaaata actagtaatt
    20341 ctgtatgaaa ttaggtacaa tatacacgtc gatgcacaag cattgactat ggcaacttga
    20401 ttggtcagtt cccgctgact tttcaagccc cgacaattta gctgctacta aattgtcggg
    20461 tgcctttttt agtttttatt cgtttttcat tctatagaaa gcgcttcaat aaatcaagac
    20521 atttttttgt tagaatgcta ataataacaa aaggttttaa ctcttcttat gtacattaca
    20581 ggtaaaaaaa caaaaaaatt tctctttttt ttgaattctg aacaaatagt actggagtac
    20641 aaaagacgag gaagagccac tatctgctct tcctcgtctt ttgattctca cctgttattc
    20701 atgattcttt ttctcagttt cccgatattt ttccatttta cgactaaaaa gctctccgct
    20761 acttggtggc gtatatgtga tggcatttgc tcctgcttga atggtctcag caatactttc
    20821 ttccgttgga ccacctgtcg caatgatagg aagttcagga tattttttac gaaaatggcg
    20881 tactgcttct gcagtttctt tacctgcact gatattgata atcgaaatac cagctgctaa
    20941 cttttcatct atttctgtat atttcgacgt taccgtactg ataactggaa tatcaactac
    21001 tcgacaaact tcttttactg tctctaaagg cgtaggaccg ttcaaaacga cccctaacga
    21061 accttgcgct tctgcgaaca tgctcatgta ggaagaacga gcaccttgtg tcaaaccacc
    21121 acctacaccg gagaaaactg gaatgtcagc tgcctcaata atgcttttag taatcgctgg
    21181 atgaggtgta aaaggataca cagcaattac tgcattcgca tttgtattac gtatgatcgc
    21241 aatatccgta gtaaagatga tcgaacggat caattttcca aatatcttga tcccgcttgc
    21301 ttctaaaatg atttccggta ctcttacgat atcttttctc aattccgtca tgatttctgg
    21361 cgtaaattgt tctgccatca gccacttctc ctttgctatc tccggtcata ctgattcaat
    21421 tgatacgtct cgatgacctg gatgattttt tgcgcataac cggggtctgt cgcataacct
    21481 gcatcttgca atgcttgtgc ggcttcctga tagttggtgg ccgtaatcac tttttcatac
    21541 agcgcaggat tccaatctac tccttggacg aacagttgtg tatggtcatc catggactgt
    21601 tcccatgaat catacacttt gaagtctcct tggatagtta tccattcttc gttaacaaat
    21661 tcctttgttt ccaggcttac tttcttctga tccccataag ccttgatccc gaaaagatta
    21721 ttgtatttgc tagccaaggt actttttccc caatttgatt ctaatatagc ttgtcctaaa
    21781 atgatactag gaagcactcc ataaccatct tgtagttctt gggcatgcgg aacaagccgt
    21841 tcaataaatt gctgatggga ttgcgcttca ttttcttgct gattatcagt aaaaggagaa
    21901 gacagattct tcaaggaaaa aacaaaggct aagagtatca ctgctacacc ggcaaccact
    21961 gctggccaac ggatattctt gcgcttttgc tttttccact tttttttcgc catgatcaat
    22021 tcctactttt catttaattc attcagatat tcttctagcg tcaattggtg tttggtgata
    22081 tactcagcgt tttctttacc gacataacgt aagtgccaag gttcatatgt gatcttagtt
    22141 atatcttcgt gtccttttgg ataacgaacg ataaatccat actgcgcagc attatctgcg
    22201 atccatttag ctccaggctg atctccgtaa cttgcatcca atacagtaga aggataactg
    22261 ttgtaccagt tttgatcgac gacatcgact gccagacctg tgtggtgttc gctgtatcct
    22321 ggctccgtaa tcgtttcttt agtaatcttt gtcgcctcct cttctgatac accttgacga
    22381 ctcatcacat cttggacatt ttgttcgaaa acttgttgtt gggatgatac agaccggtaa
    22441 gctgagacca tcactaatgg aaatccagct gcttcagcag ccttggcaaa tgcttcatat
    22501 tctggagcaa tgcgtttgtc gatcaagtat ccgttactta atgatgccaa ttgagtagac
    22561 tcatcaattt ctttttctaa tttgtgatct ggaccaacca aaactagctt ccaatcgtct
    22621 gttttaacat ctggcaattg tttggacgta cgatcttttg tttctttgtc ctttaccttg
    22681 ctgctggttg tttgactgtt cttttcttta ctagcagttg ttttggtgct tgttcgttcg
    22741 actgcttgat tgccggcttg aacatgcgaa tcatcatcgc taaaaataac atagcccatt
    22801 gctgcgatca taatcacaac tgctccaaat cctaaaaatt tattcacttg aaaaatcctt
    22861 tcgttgttct tcacgttccc cattcgcctc atgaacgtaa gtttcttcca gattttcatt
    22921 cacccaatcg agaagttcga ttttttctcc ttcgatctgt ttttgaacat ctgctggcag
    22981 acgaaaattc aaaatatcat catagcccca ttcatcatat tgaatactta ctttcaagtc
    23041 aaggtatcta ccagcctgct gattattttt caaagggatc agctcgactt ttgctgcttt
    23101 gctgctaagc catttttgcg cgatgcgtgt tttgatttgc ttgtactctg caacggcaac
    23161 gtttcgtttt tcgggaaaaa taatcgtttg acgcaaaatc ttttgcgtta gcatccattc
    23221 ataatcagtg aaaagatttt ttactttctg catcgcttca gatggcagat ttttctcgcc
    23281 atctttccat ttttgccatt gttctggact gatcagcaag tattcatcta aaaaattttt
    23341 ttcagtttca aactgttcta ataatacact cataataaga tcaatatata cttgatgcac
    23401 gtttatcccc tccttttaac tattctactt aagttttgat caaaaagcac ctcttaaatc
    23461 gctgctttca ttttttttaa tgtttcaagt cctctcctct ttattcttcg tgatttttgt
    23521 tataataata gacagacaag aaactcaatc caactatgaa ggagcgtttt cagatgaaaa
    23581 aatatcaatg gggaatcgtt ggtcttggta ccatcgcaca tgagtttgcc gaaagcttca
    23641 atcaagagac gagcgaacta acagcagttg cttccagaac ttcagaaaaa gcggaaaact
    23701 tcgctcaccg ttacaatatt ccaaaagctt acggaagcta tcaagaaatg ttagacgatg
    23761 ctgaaatcga catcgtgtac attgctgtac caaatagaca gcatatcgac catatcttgg
    23821 cagcattgga agcaggcaaa cacgttctat gtgaaaaagc catcacgatg aataaaaaag
    23881 aactggctga cgctatgcgc ttagcagaag aaaagaattt gatcttagca gaagcgatga
    23941 caatcttcaa catgccactt tatcagcagt tacgctcaat aatggataca ggaaaactgg
    24001 gggctttaaa aatgatccaa gcacctttcg gcagttacaa agaaccagac cctaaaaacc
    24061 ggttcttcaa tccagaatta gctggaggag ctctgttgga tatcggtacg tatgctgtat
    24121 ctttcgctcg tttctttttg agctctcaac ctgaagtagt tgcctctaca atggttcctt
    24181 ttgaaacagg cgtggacgaa caatccgtca ctatcttgcg taacaaagaa aatgaacttg
    24241 ctgctgtcag cttgactttt caggcaaaaa tgccaaaagt cggtatcgtt gcctttgaga
    24301 acggttatgt gacgattgct gattacccgc gtgctgatcg tgcggagatc ttatttacag
    24361 atggtacaaa ggaattcatc gaaagcggga atacatctga agcaatgaat tacgaaataa
    24421 agaatatggt acacatgatt gaaggtgact tgcccaatcg ctctctcttc ttgacaaaag
    24481 atgtcatcga aatcttggat caaatgcaag ttctatggaa aaatatgtaa ttttaaaagg
    24541 ttgtgaaaaa gctgttctgc atcaagtaat aagaacagcc aaacaaaaat agctcctcct
    24601 atttttcgtt tggctggact tattaccgca gaggaaagct tttgaacacc gtttaagaaa
    24661 gaggttgtga gcctaccgtt cagctccgag tcacaaggag caatttcaaa aaacagcttc
    24721 tcatgtttgt tgaatattgt gacttgtgac cgaggagttg acaggagaac accgtttaaa
    24781 gaagaggttg tgaaatcagc tttttgacct gaatattagc tgaattcaca acctctattt
    24841 ttggattttt tgttgggaga attctgcctt gatttcaata aaacttttaa aagactgcaa
    24901 aaactggaac aattctgctc gatcctcgaa ttcagggcga ctagtcggca gaggcatttg
    24961 ccgatacaat acataaactt cttcaatacg tgttaaaatt tgtctgccat catttgactc
    25021 tgaaaatgtt tctgcagtat aaatcaataa accatagata tgttttccat aataagctgg
    25081 ttgctggatt ctctctagat tttccagcat gtctttaatc acattggcct gtgctcttct
    25141 catagagaag taagtttcgt agtatagcgg ctgtctgagc cagtagtttt cttgatgttc
    25201 tctggcatct tcttggcttt cgcgaatgaa tgtcagtaaa tgttcgcatt tgatctgcac
    25261 atcttcttta ttttctgata aaagaaattc tgccatttgt ctcaaaatat ttcgaaattc
    25321 tttttcgatc tgtatttggt tttccttcag ctgcttcgta cgatctggca tgtaaacatt
    25381 tgcaagcaaa gcaaatacta atccaatcgc cattaatccc gcttcattgc cgatcagctg
    25441 ccaagacatg ttttcctcca ctaaataatg agtgaccaaa accgaattta ctactatccc
    25501 ttcggttaat ttgaagcgag aggcaagcgg gatgaacagg aataaaaaaa gactgaagcc
    25561 aaaaatagta tacccaacag caaaaaaact gaacagtgct gttgccgtag ctaatataaa
    25621 ggctgcgatt cgattcatgc ccgtacgcaa tgtagagcgc ttggtatttc ctacacttaa
    25681 taatgcaatg atccctgcag ctggccaata caacagcgat aagctacccg ctgtgaccat
    25741 cgaaaagaag acactgatca ctgttttgac tgttcgcatt cctatacgca tttacttccc
    25801 ccttttttat tttaattata cttgaataat gaaaaactag ctatcagaca aaagtttagg
    25861 tatgatagta aaaagaccaa aaaggagaat ctctatgcaa tttggccgtt ttcgactagg
    25921 tatgcgtacg atcaaaagtg ccttagctgt ttttttatgt atcttgtttt ttcatttgac
    25981 tcaacgagga atcccgatga ttgctgcatt atctgctgtt ttttctttgc gtcaggactt
    26041 gactacaacc gtttcttttg gaaagtctag gatcatcggc aatacattag gtggtacatt
    26101 agctatcatt tatttttttg tcaaaagcta ttttcacaat gattttttgg tagaattgat
    26161 cttgcttcct attctagtta ttattgtgat cgtcgtgtca gatggtatta acaataattc
    26221 cggtatcatt tccgcaatag ctacgttgtt gctgattgcg ttgagcatcc ctcaaggcga
    26281 atccgctttg tatgctatcc aacgtgtact agatactttt atagggacat tcattgcaat
    26341 cggtatcaat ttctttcttc gtcctcctga aacagaaaag aaggaagaac tatcagaaga
    26401 tttggtcgaa ctccaaaaga aagaacgatt gctaagaaca aatttacaag agatagaagc
    26461 taagatccaa aaacaaaaga aaaatagcga ggactaattt tcttttgcaa taaaataaaa
    26521 aaatggagag aagaacttta aaaagtcttc ctccattttt gattgaacgg tgaagtgtac
    26581 tactttattc ttccttcatc gagttttgtt ctgcctcttt tggcgcttca tacatattta
    26641 cttggctagt agtcccacgt gtcgacaaat tctctacgat ttccttcaag tcaagtcctg
    26701 ttgtcgcttt tagtgtttct tgagtagaag aaagcaaatt tgtggcataa tttgtcactc
    26761 ggttcgctcc actggtttct cctgaactgc ttcctgtatc gacaacagag attttttcga
    26821 tatttcctag cggctgtgct gcttcgcgca tcaattgcgg tagcatatcg ataaccatac
    26881 tcaagacagc tgcttcgcca tattccttga aggcattggc gatcttttgt tttgcttcgg
    26941 cttctgcttt tccttttgct agtgctgctt cggcttctgc ttgtccagct aaacgtgtct
    27001 tattggcttc cgcttctgcc agtgcttcca cacggaatcg ttctgcttcg gcttcagtca
    27061 cttctttgac tttttgagct tgtgcttctt gttctttagc gtaccgatcg gcatctgctt
    27121 ttttcttcac ttccgaatca tattgctttt cacgacgtgt gatttctttt tcttccagtt
    27181 cgatttgttt ttgacgttct acgattttaa tctgcatttc ctgttcgatt acttgttgtt
    27241 gtgctcgggc actttctaaa ttgtaggctt ggtctgcttt tgctttagca acatcttgtt
    27301 cttgtttgta ggttgctaat ttcagttctt tttcttttaa tgattctgcg atctctgttt
    27361 gtcgctgtaa ttcagctgct tgtgattctt tttctgcttg agcttttttg atacgcgttt
    27421 cttttagagc ttccgcttct gctatatctg catctctttt gacttgggcg attcttggtt
    27481 tccctaaaga gtcaagataa ccgtttttgt ctctgacctc tttgatagtg aaagaaacga
    27541 tgatcaaccc catttttgca aggtctacag aagcgacttc ttgtacgctt tgactgaact
    27601 tatcgcgatt ttgatagatt tcttctacag tcatagaacc aagaatcgag cgcaagtgtc
    27661 cttctaatac ttctcttgct tcattttcca gttcttctct tgtcttgcct aaaaactgtt
    27721 ctgctgctgt agcaatctct tctacagatg aaccgatttt gatgatcgaa gtaccatcac
    27781 acatcaccgg tacgccttgt tccgtataca cttcaggggt ggagacgtcc aatttactcg
    27841 ataatagact gatccgattc gaacgttgaa aaactggcaa gacgaatgcg ccacctccac
    27901 gcacaatttt gattttgtta ttgctttcat caatgtgcac atttttgctt cctaaataac
    27961 ttccactgat gatcaacgct tcatccggtt ttgctgtctg gtacttcgct acaaagacga
    28021 tcagcaacat cagtaaaatc aaagctgcga ttgcgatcgc gataaaaaca actggtattt
    28081 ccatttaaca cacgctcctt taagctttta tcatgctttc ataaggcatg acataacaga
    28141 ttctttcctt gatctcaatg atcaatactt tcgttccccg ctcagctgtc tgttcttggg
    28201 ggctgtaaaa acaagctgga cgactaatgg aaccggtaac acttttcaac tgaatttctc
    28261 ccatacctcg tacagggatc ggtgtgatca caacagctac ttgtccttcc agagtttttt
    28321 cagaagacga catcgttgat tcggcatgtt tcatcggcat caatatatag aaattaagca
    28381 aaaataccaa cactatcgat atgcctccac tagcaataaa taaaaataaa gatgattgat
    28441 ctgttaaacg ctcacctaga aaaccaaata agcttgtgaa agttaaccaa gggataataa
    28501 gcatcggatc aagcgggcca tcaaaatcga atacatctcc aaaaataatc aataggactg
    28561 ccaatcctgc acaaatcagc aatgtatacc aataaatcgt ctcaagtgcg attcccccta
    28621 tcatatacac gcctccttcc atttctacgt ctatcatacc aatgtttgaa aattctgtat
    28681 agcttctttt accgactcag tggtgttttt tataggaagg ttgtgtttat cgttgacaat
    28741 ttgttaaatt tttgttttca tgttaaaatt ttcacaataa attctttcga ggtagcctat
    28801 gtttcctttt tttaagaaaa aaccattaaa gctccagaac acgatcatcc ttgtcgtttg
    28861 tttctccaca ttcgtttctt tgattgtttc tgccatattg atcaggaatt ttattctttc
    28921 gatgaaccga tctagtgtgt cagataaaat ctctagcatt gctaaaatcg ttgccaatga
    28981 taaactcatc caaaaaaagc tgcagactgg tgacccgaca catgcgattc aaggaataac
    29041 aaatgaaatc atgattgcat cgaatttaga ttttatcgtt gtcatggacc ctgaaggcat
    29101 tcgatattct cacgcagacc caagttacat cggtcttcct ttctcaagtc atgaagatgt
    29161 gaaaaaagtg attggaggac aagaattttt ctctgaaaaa gaaggaatgt tagggttcgg
    29221 cttccgctat ttcgtcccta tttattatga tcaagagatt attggtgcag tttgtgttgg
    29281 cttaacaaac aacacgattg acaatcaagt caaagatgga caaaaaatga ttgttttttc
    29341 attgattatt ggacttagcg taggcatcat aggcgccgtt tttcttgccc aaaaaatgaa
    29401 aaaagtactg cttggacttg aaccacaaga aatagcagaa cagttaagtg aaaaaacgat
    29461 tatcgaaaat gaagtagatg aaggaatctt agccatctct tctgataaaa ctatcctctt
    29521 gatcaataca cctgcaaaaa acattttcta ccaaacagag cagctattgg atttcgaagt
    29581 aggaaaaaaa tcagtgataa tctatatgac gttttgttta aagagacgat tgagcatcaa
    29641 acgaccgttc ggactcaatc catgtattta aatgggatag aaactatttc tagcgttaca
    29701 cctcttttta gcaatgaccg ttttttagga gcagtcgcaa ctttacgaaa tcgatcagaa
    29761 gtaatgaaat taatggagga gcttgggggg acgaaacaat atataaactc catgagagct
    29821 caaacccatg aattcatgaa taaacttcat gtcatttctg gtttgattga tttgaagaag
    29881 tatgaagaag tcacccattt tatccagcag ctaaatcaaa attataaaaa agagattggc
    29941 caagtctcaa gtctgatcaa agtacctgca attgccggtt ttttattagg caaaataaac
    30001 gaagccaaag aacaatctgt tgaaatgctg ttagaaagcg attcttttat ccctaaaatg
    30061 gagatggatg actccatcca ttcacttttg caagtgttag ggaacttact tgataatgca
    30121 aaagaagccg tgctacaaaa acatgaaaga aacggacagg tccggattca tttaaactat
    30181 gacacagaag ctgaagtatt catcgtgacc gtctgcgatg acggtgtcgg gatatcagaa
    30241 gaaatctcag aaaaaatatt tacgctaggt ttttcgacga agggaatcaa tcgtgggttt
    30301 ggattgaatc tcatccattc aatcgtcaaa cgccacggtg gatttttaga attttccaaa
    30361 caaacaaatg gcggtacaat cgtctatcta gaattaccaa aaaaggaaga tcaaaatgat
    30421 gacaaatgtc ctgataatcg aagatgatcc aatggttgca gaattgaatc gtacttttgt
    30481 ggaaagcatc cctaatatga acgtaatcga aagtttcaga aatgtagaag aaggaaaaaa
    30541 atatttcaga caaaaaaacg cagatctgct gctgcttgat gtatacatgc ctggcgaaag
    30601 tggtatccat tttttatctt ggcttagaca gcaaggaaat gatatcccag tgattttgat
    30661 cactgctgca gatgatatcg aaaccataaa gaatgtgatc cgctacggtg ccttagatta
    30721 tttgatcaaa ccgttcactt tgcaacgttt tcagctggcg atcgaaaagt atcttgcttt
    30781 ctctcagaca atccaaacga ctgaaaaagc cgatcagaaa ttgatcgatc aagtattcaa
    30841 ccacaccact cccattcacg aaataccatc attcatcgat ttgcctaaag gtctgtcaaa
    30901 actgacttta aaaaaaataa gtgcatttat tctaaaagaa gatactattt tttcaacaaa
    30961 agaactggct caaaaaagct cactgtcacg tatatccgtc aagaaatatt tgaatttttt
    31021 agtagaacaa gatatattga acgaaagaat caaatatttg gaaatcggcc ggccgctaac
    31081 ctgttatgaa ttgacaaaga atgggcggaa aaagctcaca gattactgtt gagtcatcga
    31141 attatcccat acaaaatagt ccaagtatac agaagtaaca actgtttact tggactattt
    31201 ttaaacattt tattgacgaa tatccatgtt ttcttatctt aattgacggg ctttaggaag
    31261 agatacctca tttttcgttt ttccagtttc tattatttca cgaaagttct cgaaacttgt
    31321 cgaaatcata tttgataatg cctcatctgt atttgaacct acatgcgggg ttacaagaac
    31381 tctagggtac atttctacta attgctggat agcagggtct ggaatcgtct cccatggctt
    31441 gaaggatcgg aaaaaaacct ctgtttcatt tgcaaaaaca tcggttgcaa aaccagctaa
    31501 atgattgctt ttcaaagctc ttaaaatagc ttcattatct tgaagctctc cacgggctgt
    31561 attaatcaag atgctacctt ttttcatttg agctaaaaat ccttcattga tcatttgatc
    31621 gttttcacct gggatatgag gtacatgaat actgacaata tcgcttttgg ctaataactc
    31681 ttcaagcgag tggaacgtca atactttttt agcagcttct gtctgatgaa tatcgtagcc
    31741 gatcactttg gctcctagtc ccttaaacaa aacagcttcc gtcaacccta tcctgcctgt
    31801 tcctatgatc cctactgtgc aatttctgat ttcacgacta aacatttgct catccactcg
    31861 aaaatctttg aaagatgttc ggatcgtcgt atacgcagta tttctcaaaa gcatcatggc
    31921 aaaagtcaaa gataactctg ctatcgcatt tggcgaatac gatggtacac gggctactgt
    31981 cataccaaaa tcagctgccg cttgtagatc gatatgattg actcccacag tccgtgtaaa
    32041 aacataacga atcccatatt cctgcatttt tgcaagattg gtctcatctg ctatacaatt
    32101 tcccctcaac aatacagcat catggtcctt tgctgacacg atattgtcat gtgtcaacag
    32161 ttcttcataa agactgagat cataatggta aatattcaat ttttcaaaaa aatctatctc
    32221 gttcggacga acaccgtaac atgcgattct catactttct tcctccattt cttatgaaaa
    32281 taaacctatg ccatgaatca tttgtaatac aacgatccat acaggtgtca gtaccacagc
    32341 aaaaatagtt gacagtaagg atgcattgga ggctaaaagt gcctccctat caaaactgat
    32401 agcataggct gctgccactg ttgcagctgg tgtagccatc atgataacga ttgttgccag
    32461 tgcttcaaaa ttgactggta gaatattcgt tgccgtcaaa actgctagaa tcacgatatt
    32521 caatgcaggt acaaatacca ctttgactag actgtaatac catgaagttt tttcagatgc
    32581 agcttttttg aagcttactt cacctaatgt tgcgccgatt gctagccacg ccaatggtga
    32641 acttaaacca gctaaataat ccatcgactg aaatagccac ggtgcagtct ggtcaattcg
    32701 caaaaacgct acttggctta tctctccttc tgcacctgta actgacactt gtggcagata
    32761 cccttggaac agccaaatga aaaggccggc aaaagtagcg ataatgattg gattgacgaa
    32821 catcgctttg atatttttag cttccatctt caagccactc atttttatgt atccataaga
    32881 atacaaaaag attcggtagc caatattgaa aattgaggaa tacatcacgc cgacaggacc
    32941 ataaatcgca ctgacgatag gcgttccgaa aaaggtcgta gaaccaaaga tcgtcaggac
    33001 acgcaacgtt gtttgcttat cccctttata gcttaagaaa aaaggttttg aaactagaat
    33061 aagaataata taaataacta atccccaaat caagacattg attccttgca tcagtaattc
    33121 tgtattgata tcttgcatga aagctcgaaa tgccagtgct ggcaaagcca ctgttaagac
    33181 gactttcgat agaatttttc caacagattc agaaaaaatc cccttttttc tacaaacaaa
    33241 ccctaataat ataataaaaa ctgttgaagt gattgcactg acaatcgaca tatctgtcaa
    33301 agtcgatcga aaaatgtcaa ataagttcat ctttctcctc ctaattatgt tcatttattc
    33361 acaataaaaa aagcttttat tttgctaata cgtattaaca tttttaattt tatctttaca
    33421 ttgttactta ttgaacaata ggaaattaaa gaaacttttg aaacttatgt aaagtgatt
//
LOCUS       DAZKOU010000022        33079 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000022 DAZKOU010000000
VERSION     DAZKOU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.022"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..485)
                     /gene="yajC"
                     /locus_tag="ACG827_001004"
     CDS             complement(105..485)
                     /gene="yajC"
                     /locus_tag="ACG827_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0483164.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(582..1727)
                     /gene="tgt"
                     /locus_tag="ACG827_001005"
     CDS             complement(582..1727)
                     /gene="tgt"
                     /locus_tag="ACG827_001005"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0483165.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1945..2643
                     /locus_tag="ACG827_001006"
     CDS             1945..2643
                     /locus_tag="ACG827_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0483166.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2811..5450)
                     /locus_tag="ACG827_001007"
     CDS             complement(2811..5450)
                     /locus_tag="ACG827_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0483167.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6069..7100)
                     /gene="queA"
                     /locus_tag="ACG827_001008"
     CDS             complement(6069..7100)
                     /gene="queA"
                     /locus_tag="ACG827_001008"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0483168.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7293..8467
                     /locus_tag="ACG827_001009"
                     /pseudo
     CDS             7293..8467
                     /locus_tag="ACG827_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            8471..9112
                     /locus_tag="ACG827_001010"
     CDS             8471..9112
                     /locus_tag="ACG827_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0483169.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9109..10026
                     /locus_tag="ACG827_001011"
     CDS             9109..10026
                     /locus_tag="ACG827_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0483170.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10023..10694
                     /locus_tag="ACG827_001012"
     CDS             10023..10694
                     /locus_tag="ACG827_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0483171.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11235..11723)
                     /locus_tag="ACG827_001013"
     CDS             complement(11235..11723)
                     /locus_tag="ACG827_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0483172.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11924..12190)
                     /locus_tag="ACG827_001014"
     CDS             complement(11924..12190)
                     /locus_tag="ACG827_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0483173.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12190..12453)
                     /locus_tag="ACG827_001015"
     CDS             complement(12190..12453)
                     /locus_tag="ACG827_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0483174.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12974..13114
                     /locus_tag="ACG827_001016"
                     /pseudo
     CDS             12974..13114
                     /locus_tag="ACG827_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13256..15079)
                     /locus_tag="ACG827_001017"
     CDS             complement(13256..15079)
                     /locus_tag="ACG827_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0483175.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15677..18382)
                     /locus_tag="ACG827_001018"
     CDS             complement(15677..18382)
                     /locus_tag="ACG827_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0483176.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18688..19635)
                     /locus_tag="ACG827_001019"
     CDS             complement(18688..19635)
                     /locus_tag="ACG827_001019"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0483177.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19825..20094
                     /gene="rpsN"
                     /locus_tag="ACG827_001020"
     CDS             19825..20094
                     /gene="rpsN"
                     /locus_tag="ACG827_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0483178.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20270..20632)
                     /locus_tag="ACG827_001021"
     CDS             complement(20270..20632)
                     /locus_tag="ACG827_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0483179.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20643..21764)
                     /gene="alr"
                     /locus_tag="ACG827_001022"
     CDS             complement(20643..21764)
                     /gene="alr"
                     /locus_tag="ACG827_001022"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0483180.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21780..22130)
                     /gene="acpS"
                     /locus_tag="ACG827_001023"
     CDS             complement(21780..22130)
                     /gene="acpS"
                     /locus_tag="ACG827_001023"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0483181.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22260..23771)
                     /locus_tag="ACG827_001024"
     CDS             complement(22260..23771)
                     /locus_tag="ACG827_001024"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0483182.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24093..25445)
                     /gene="murF"
                     /locus_tag="ACG827_001025"
     CDS             complement(24093..25445)
                     /gene="murF"
                     /locus_tag="ACG827_001025"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0483183.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25598..26674)
                     /locus_tag="ACG827_001026"
     CDS             complement(25598..26674)
                     /locus_tag="ACG827_001026"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0483184.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26870..28615
                     /locus_tag="ACG827_001027"
     CDS             26870..28615
                     /locus_tag="ACG827_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0483185.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28895..29332)
                     /locus_tag="ACG827_001028"
     CDS             complement(28895..29332)
                     /locus_tag="ACG827_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0483186.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29316..30689)
                     /locus_tag="ACG827_001029"
     CDS             complement(29316..30689)
                     /locus_tag="ACG827_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483187.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31011..31499)
                     /locus_tag="ACG827_001030"
     CDS             complement(31011..31499)
                     /locus_tag="ACG827_001030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0483188.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31521..32123)
                     /locus_tag="ACG827_001031"
     CDS             complement(31521..32123)
                     /locus_tag="ACG827_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0483189.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(32192..>33079)
                     /locus_tag="ACG827_001032"
     CDS             complement(32192..>33079)
                     /locus_tag="ACG827_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0483190.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT         9448 a         7026 c         6060 g        10545 t
ORIGIN      
        1 aatattgagc aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta
       61 ggacatcttt ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc
      121 tcttctgtct cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt
      181 tcttctgttg tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc
      241 gttttgattg ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc
      301 cgtttgtcat tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa
      361 tcacctggtt tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa
      421 cgtgacataa aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt
      481 cccatttttt tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct
      541 gataacacta gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt
      601 tctttattga acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc
      661 atgattgctt gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa
      721 gtcaaacgaa tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta
      781 tagtttttac atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat
      841 ttcgcatttt tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt
      901 cgagtaggca agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa
      961 tcagcagcac cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg
     1021 aattctaata cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag
     1081 taaccaggga aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac
     1141 cctgctcctt ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat
     1201 cctcgttctg cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca
     1261 tcgaatggtg gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg
     1321 ttgattgctt tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc
     1381 acgccttctt cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa
     1441 tctgttaaaa tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact
     1501 aagtcttcac cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc
     1561 atttctttca actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg
     1621 aacattggtg tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta
     1681 tgtttttctt tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact
     1741 ttctatataa aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa
     1801 gttccctcgg cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat
     1861 aacaaaaaaa agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt
     1921 attaaagaaa aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa
     1981 gagagtctaa aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt
     2041 ggtattgcta ctattcttct tattatgatt cctattatct tgtttgccac caccatatat
     2101 gatggatcag cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt
     2161 ggcggaatta tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat
     2221 cttgatattt tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga
     2281 ttcaaaggtt tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca
     2341 acattatggg gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa
     2401 agctatttta tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat
     2461 acgatcactg caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat
     2521 ctttcattta tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg
     2581 ctcagcccat acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt
     2641 taaaagagag tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc
     2701 agtaaaagaa acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc
     2761 tcctttttgt atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct
     2821 tcttctgctt ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata
     2881 gataaaatcc ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct
     2941 tgcgtaaatg gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct
     3001 accaaagctt tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg
     3061 aacacatgca gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc
     3121 tgacttgcgt ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac
     3181 aaggttacga tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca
     3241 aagatccctt catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct
     3301 cttcctaaag caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca
     3361 gctgtaaatg gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa
     3421 atattacagc ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct
     3481 tgactgacag cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca
     3541 ctttgcgcca catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac
     3601 gctggtgcat cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa
     3661 gatttgacga tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg
     3721 acacgcgcat ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct
     3781 gacggatcat tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct
     3841 ccagtgatca tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt
     3901 tctggacgtg gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt
     3961 gcttcagaag aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact
     4021 ctcagtgccc gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa
     4081 ctaaagcgcg gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc
     4141 atttggtgaa ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt
     4201 ggatattttt cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg
     4261 gctacttctg ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca
     4321 tctgtacata gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg
     4381 atattcgtta cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc
     4441 agcgttcctg ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc
     4501 aactgtcgga tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac
     4561 gtaacaataa cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg
     4621 aacatctcaa ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc
     4681 gctaaagcga tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt
     4741 ttttgcatag tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca
     4801 atggcagtga cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata
     4861 ttaagttggt cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact
     4921 gcttcacttt ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa
     4981 cgggcatcgg cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt
     5041 tgctctgact caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc
     5101 atatcttgca aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc
     5161 gagttgatca caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta
     5221 gcaaatgcgg tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg
     5281 gacaagcttc gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc
     5341 aatccctgct tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct
     5401 gcttctgtta ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc
     5461 ctttaaaatc ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta
     5521 cttcaaataa agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata
     5581 tttgcaaacg ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa
     5641 tagacagaag aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt
     5701 ttcacccaga catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg
     5761 gatcacgaag tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg
     5821 tctgaaaatt gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa
     5881 tttttgttta acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa
     5941 tcatttctgt ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg
     6001 gctttttctt tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt
     6061 tcataacctt atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca
     6121 atcgcatgct ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg
     6181 actaaagtcg attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca
     6241 tagccaggcg taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat
     6301 ttcgttccaa tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg
     6361 ccatttttac gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca
     6421 ctatgcatat ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct
     6481 acatgtagtg tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct
     6541 ttcgtaaaat gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca
     6601 gtttgatagc gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct
     6661 cccaatgatt caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg
     6721 ccgtgttcca attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt
     6781 gtcccaactt tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta
     6841 ttcgttagaa gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga
     6901 gcaggcagaa ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg
     6961 atttcatgga aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga
     7021 gaatgatcgc ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca
     7081 aaatcttctg ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc
     7141 atgtttttcg ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa
     7201 ataaaaaaac taccctattt gtattttttc attgttttag gcatactagt aatagatagt
     7261 tcttcattga aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa
     7321 tttataaagg tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt
     7381 tcatttgttt tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc
     7441 gaatgactga tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg
     7501 atccagtgga attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc
     7561 acatgacgat ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag
     7621 aacggaataa aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag
     7681 atcgctaccc acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc
     7741 ttgcagcgaa tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca
     7801 ctcgtgattc cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg
     7861 tatttgtaac ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga
     7921 gtgaaggccg tgtgatcaat ttgatacacc agaaaatatt ttacgtcatc ctgcaaacga
     7981 tttcgtagaa gagttgattg gagaagatcg cctactacaa gccaagccaa atgcgacaag
     8041 agtcggcgaa gttatgctaa atacagcaat cacgattaca ccggaaaaat cattacaaga
     8101 agcaatcaaa ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg ataattcgaa
     8161 tgtattaaaa ggatttatcg atgtagaaac gttgaataaa aaacgaggaa aagtatccag
     8221 tgtcggcgac attttgaata aacatgtttt ttacgtaaaa gaatccgcct atcttcgtga
     8281 taccttgcaa cgcattttaa aacgcgggct gaaatacgtg ccggtcgttg atgaacagaa
     8341 caaagtcgta ggtatcctga ctcgtgcatc attagtggat atcgtctacg atgtgatttg
     8401 gggagaagat cccgcaacta ctgatgttgc agagtcagct actcctgaag aatctaagga
     8461 ggtctgacct atgcagcagt tttttcatca gcacggttca gaaattctct caaaaggttt
     8521 tgaacatctt tatatttcag gacttgcttt gatcattggt atcatcattg ctgtaccgct
     8581 tggtgtctta ctgacacgtt ttccaaaagc tgccacgatt gtgattggct taaccagtgc
     8641 cttgcagaca atcccttctc ttgctttatt ggcattgatg atcccgctat ttggcgtagg
     8701 taaattacct gcgattatcg cactctttat ttattcatta ttgccgattt tacgtaatac
     8761 gtacattggg atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag gcatggggat
     8821 gactgagttc caatccattt tttccgttga attgcccatt gctatgccaa cgatcatggc
     8881 tggtattcgt ttagctgccg tttacgtgat tgcatgggct acacttgcct cttatatcgg
     8941 cgctggtggt ttaggcgatt tgatttttag tggactgaat aattaccaac cagatctcat
     9001 tttggcaggt acgatccctg taacaatttt agcactattg gctgattggc ttctaggtct
     9061 cttggaacac cggctgacac cgatcgcact tagagaggag aatgaagaat gaaacgtata
     9121 ctaaaaatga ccatggttct tttctctctt cttatacttg cgagttgttc cttccctggt
     9181 cttgccagca atacagatga agacaccatt tcgatcacag gtggaatcac ttcagaagca
     9241 cagatcttag caagtctagt tgccggaatg gttgaacatt atacagataa aaataccgca
     9301 atcatcaaca atctggctac tacaactatc aaccatcaag cgatgatgaa cggagatgct
     9361 tcaatttcag cggctcgcta cacaggaaca gatttaacga ctacattgaa ccttcctcct
     9421 gaaaaagatc cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa aagatacggt
     9481 caaacgtggt tcccgtccta tagttttgaa aatacgtatg tctttcttgt tcgaaaagac
     9541 accgcacaaa aataccatct atctaaagtc agcgatctga aaaatgtagc ggatgaatta
     9601 gtagctggtg tagatacttc ttggatcaat cgtaaaggag acggttatga cggcttccaa
     9661 gaaacttatg gcttctcctt caattcgata ttacctatgc agattggact tgtttatgac
     9721 gcagtcgaag caggaaaaat ggatattgtt cttggctact ctacggatgg acggatcgcc
     9781 agttatgatc tagtcatgtt ggaagatgat cgtcaattct tcccaccata tgatgctgct
     9841 ccagttgtag acaacaagat attagctgat acgcctcatt taaaagaagc cttaaataag
     9901 ttaggtcata ccatcagtac ggaaaaaatg caggaattga actacgaagc agataataat
     9961 ctagtggaac catcagtagt agcacaacgt ttcttacaag aaaaccacta ttttgaaggg
    10021 aagtgaagca attggataaa atgaatgtct ttcaacaatt tttctattat ttccaggaaa
    10081 atggctccta tatctttgca caatttatcc gtcacttcct gatttctatc tatggtgtcc
    10141 tctttgcagc agttgtcggg attccggttg ggatcatgat ttctaggaga cgtaaattag
    10201 caaactgggt gattcgtttg gcgaatatca tccagacgat cccttctcta gccatgatct
    10261 ccattttaat cattggatta ggcctgggag tcaatgtcgt gattgttact gtatttttgt
    10321 attccctctt accgatcatc aagaatacgt ataccgggat gatccaagta gacaaaaaca
    10381 tccttgatgt cggcaaaggc atggggatga ctgctagaca acgactattc atggtagagt
    10441 taccactctc tgtttctgtt atcatggcag gcataagaaa cgcgttagtt gttgccatcg
    10501 ggataaccgc gatcggtgca ttcgtcggtg ctggtggtct tggagatatc attatccgag
    10561 gaaccaatgc aacagagggc acatcgatta ttttagcagg tgcattgcca actgcattaa
    10621 tggcgatcat cacggactgg cttttaggta ttttggaaag acggctagat ccagccagcc
    10681 gtacatcacg ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt gttggtcgca
    10741 aaggcttttt tagttctgtc atttttaagg caataatcca ctctgattct tccctcctta
    10801 tgggcaaaga gttgcttttc ttagctattt atccattccg tatactccaa cttcatactg
    10861 ccaacgggtt catatctgca tccaccgact aagttttttt cttatccgct aaggcatttt
    10921 gccaatccat tttcccatca ctcgtcatgg aattcatacc acatgatctc taaatactcg
    10981 tctgcttgtt ctttgaattg ggcatcaatt cttacatcct ttggcgtggc tttcatttaa
    11041 gccttttcca tcagtcctcg attgttcgat gccttcagct gcatcatcaa aactggtgac
    11101 tgccgtatcg gtaacttcga tcatctttta tacaatcggg aacttgccat cctcatcctt
    11161 ggctaaaaac atatctagcc attccagctt ctcgccatgg gaggtttgcg gttcctaaaa
    11221 ccggtacacg attcttatgg actgcatccg tcgcaactag acttggcgat acgatgatcc
    11281 ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat attgccaata agaaaagaca
    11341 ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt tgatactgct attcatggcc
    11401 gaaagcgcca cgactttaac ttcttccgtt tgagtgacat tcatgttcgg caatgtcgct
    11461 ttggataccc gctctgctaa aggaacgaca ctggtattgt atttcgtatc tgtatctttt
    11521 ccaaaagacc atgccaacga atcctctggg aaccaataag acgactccgg ttgattgtcc
    11581 attgcttttt tcaacatact tttggctgtt tctcgataaa cgacatttcc aattgtcttc
    11641 gcctcacttt ggcgataatc gatggtcgtt gccactgcca acactaacaa cgatgctcca
    11701 attttactga ttttccttgt cacacttctt cctccttgtt ttacataaac ccgtcaacat
    11761 tccattccta aactcaaaca atgacccttc aaaggtggag atataggaac aaattaaaga
    11821 aatcatgcaa taaatccaag aaggttggtt ttagaacaaa aaaagagtct cctttttggg
    11881 gagactcttt gttaactatc taaaaaggtt ggctcttctc acttcactca taatgtgtcc
    11941 aggcagaata aatttctaca actttatttt cctcatcaac tttgtaaacc actcgatgct
    12001 gtctattcag cctgcgtgag tatctgcctt catgtgtagg tctcagcttt tcaaaagact
    12061 gcgtgggcat atatggatct tccttcaatg tctgaatcac ttcttcaaat tgtttcttta
    12121 aattcgtttg cttgattttt cttaaatcca ctttcgcaga gtttttgatt gcgactgtat
    12181 agttactcat caaagagctt cccaatcaat atcatcgata ttcgtaaagc tactgtcgtc
    12241 tttttcgcgg tctcgtactt tatctaaagt acctgtttgc tcaagaaaaa gggtttcttg
    12301 aatcgctctc cagtcttcca gcccaatcaa tacagcattg tttccactac gatcactaat
    12361 aatctcgatt tcagtatgat tttcatttac ctctttcaat aactgataaa aatttttacg
    12421 agcagtgcta ggattcatcg ttgtattagc cattgtctct cctccttttt ctgatataat
    12481 tatagcgtac ttgattaagt acgtcaaaaa aatacaataa agcacttaaa ttcagcaaac
    12541 ttttcctctt ttttacatag agaaaaagac ccctttttag ggaggatctt tgtccacgct
    12601 cacttcttac tcctttacga ctagcggtag ccaaacgtaa agtaattggt aacaacgtat
    12661 catctttagc aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac caatacacga
    12721 aggttcctcg ttgcagtgtg acttgattga tatcttgaat aacctgagga tacgcacgaa
    12781 agatttgtaa cggttattca aaactgattt tggccgtcaa gtcttctact gcctcaaaaa
    12841 tgattgcctc tttcgtctcc tgtcattcat gttcactatc gatttgatac ttgcttagac
    12901 cgttgggttt gtgaatcacc aattaaaaat ggcactgagt tatggtaaca aaaaatggtc
    12961 acttgacgag gctgagattc ctacttatga agacacatgg cacttaagag tcgaggtaaa
    13021 ggaagaagct gaaaatatag gaggctgtac attatttggc aaaggattcg gaaattatga
    13081 tcaagacatc aaaatcaaaa tgttttacag ctaaatagga agaataataa catttcatac
    13141 gctgttccca tctttaatgg cttatagagg gcacctttag ctgagaataa gacaaaacag
    13201 accctctagc ttcaattaag agctagaggg tctgtttttg attttaaaac ttggtttatt
    13261 tcttcaaatg gtagctatag gtgctgacaa cgatattttc acgccaagac aatcgcaatc
    13321 ttaaccgcaa acttgtttgg ttatggagaa tattctgcca gcgtctgttt ggtacaaatt
    13381 gaggaatcaa cacagtagtt gtatagtttt gtttcgctgc atttttactt acgatatcta
    13441 cataacggat aattggattt tcaatcgagc gataagaaga gtggacgatt gaaaaacgga
    13501 catctgggaa atgcttttta aattctgctt cgatttcttt ttctttttcg acatcttcgt
    13561 ctagcgaaac atgcatcgca acgacataat caccgatcga acgtgcgtaa ttcaacgcac
    13621 cgatatttac ccgtgtcaca ttcccgacta atacaagaac ggtattcccg tcataatcat
    13681 gtagttttgc atcattttct aaacgaagct gttctgctac ttttttataa tgatcatgga
    13741 tcttataaaa aatgaacata acgattggca taatgatgaa gaaaggccaa atgtccccta
    13801 accgatagac aaacaaaatc gcaatgattg catatgagat gaacgcaccc gtaatattgg
    13861 caatcgattt gcttaaccat ctttttcctt cttttttcca tttgacgacc atccctgttt
    13921 gagataaagc aaatggaatg aacacgccga tagagtataa cggaatcaag cgttctgttg
    13981 agccatggaa aataaacagc aatacgatcg aacctagcgc tagtgtgata atcccattgg
    14041 aatagcctag tcgatcccca cgatcttgat acatgtgagg catgaattta tcttttgcca
    14101 gattgtatgc aagaactgga aaagcagaaa aaccggtgtt agcagcaact gctaaaatca
    14161 gcgctgtagc aaattgaagg acgtaataca aaataccatg tccaaagaca gctttaccaa
    14221 tttgagacaa aacagtgact tctttttcag gaacgatccc ataccaatag ttgataaatg
    14281 tgatccccac gaagaagaaa cctaagatca atgccatcat cgtcaatgta gcagcggcat
    14341 tttttgctct tggcttctta aagaatggta cagcattact aatcgcctcc accccagtta
    14401 aagaggaaga accagaagaa aatgctcgta aaatcaaagc gatagaaaca cccggcacag
    14461 ccgctcctgg aagtgcagta gcttgcaatg gcacagcacc tgtcacgata ttatataaac
    14521 caacaacgat cagtaacgag atcacaagga taaatgtgta caccggaaat aataggaacg
    14581 atgcagattc cctcagccca cgaagattca gcatcatcag tagcaaaacg attgtcacag
    14641 aaatagcgac ttgatgaccg tagagtgctg gcaccgctga tgtgatcgct tcagcacctg
    14701 ctgagacaga tactgctact gtcaacatgt agtcgatcaa taaagagcca ccggaaatca
    14761 agccagcatt ctttccaaga ttctcactgc tgacgacata tgctcccccg ccatgaggat
    14821 aagcatggat gatttgacga tatgatagcg ttaatgaaat caataaaata ataacaaaag
    14881 cggcaatggg tagcgaatac cagatggctg cagcagacaa agcgactaaa acgacaacga
    14941 tttgttccgt accatatgca atagatgata acgcatcaga cgacaataat gccaaagcag
    15001 caaatcgggt taatttgtgc tcatcatttt cagcagattt taaaggctta cctactaaca
    15061 acctttttaa gtaatccaat ttctaaactc ctttacacac aattttactt caagttttat
    15121 agtcgaaaat ttatagtaaa caagatgtta tagtacgctc actgacctta aaagtcaata
    15181 aactttaaga aaataattgg aaaaaaacag aaaaatatca agcgcttttt caaacgcttt
    15241 aatacagcaa aaatctaaca tttttttatg ggctttttct ttgaattcta taataaaagg
    15301 atagaacttg acttagaaaa tcaagttcta tcctttttga tatcttaact tttggaagag
    15361 agatgtaagg ctgctttcta cccatcattc ttactactcg aagcatagcg cagttgtcac
    15421 aacctcttga taaacggtgt ttaaaagctt gcatctgcgg taataagccc aaccaaatta
    15481 aaaacataag aagctgtttt tatttggtcg ttcttattac ttgatgcaga gcagcttttt
    15541 tacaacctct tggtacacgg tgttcaatca gctgaccctc ggtattagct caaaaaaacg
    15601 gcaaaatatg aggaactatt tttccatttt tttctctaat actcaggtca aaacactgat
    15661 ttcacaacct ctttttttaa tgaaaaatat ggctctcatg cgccgcttct ttgaatttcg
    15721 tcattttttc ttcgatgaac ggatagattg ccgttcctac gattccaaag acaacaaaca
    15781 aaccgatcat gatccagatt gctttcgtag catttggcca ataaattcct cctgcagatt
    15841 cccgtaacaa gtttaccgca tgagtaaatg gcaagaacgg attgatcatt tggaagaatt
    15901 tccctgagac ttggatcgga tagttcccgc ctccgccaga gatcgacagt actaagatga
    15961 taatagcgat ccctttaccg acattatcga ataatgcagc caagacatag acgatcatca
    16021 tgaacgctaa ggcgatcaag acagcaaatg acacgctgta gaccggattt tggacatcta
    16081 ctcctagtaa gaaaatattc cctaatgtaa cgatcaagct ttgcgcaatc gccattgtca
    16141 aaaatgtcag catacgagca acgaattgtt ctcgtttcgt aaaattgttt cggtctttct
    16201 ttcctaaata gtattctgtc gtagcaacac ttgaaagaag aacagcgccg acccacagac
    16261 atagtgcagt ataaaacggg gtgctagctg aaccgttatt tgcgattgga taaagcgcat
    16321 tggtttgtag ctcaacaggt tgtgtaaaga agtcactttc ttttgttgcg tctagtttca
    16381 ataatttgat gacttcgcct agatcgactt cttgttcgcc ttttcggatc gcgtctgctg
    16441 ctttttggat accagctttg atatttggcc agtcattgtt gatcaagtcc gcagcagcgt
    16501 tcacagctgt ttcgacttct ggcatttttt catttactgt tgtcaatgta ccggtgattt
    16561 cattttttag tgctggccaa tcattttgga taaaatcagc ggccaatcct aatttttgct
    16621 gaatgatcgg taattcattg ttgtataggt ctgccccttc attgatgcca ttgacgatgg
    16681 tatccatatt tccattcagc attgtattgg catcatggat ttcttgtcca attgcaggca
    16741 tttctttttg atatttttct aaaattgcca ctgcattcgt tacagttgct ttcgttgagc
    16801 tgagaagcga atcaaagtcg atctggtccg ctttgctcag tgcgccttgt gcagtcccaa
    16861 tcgtatcgat gacttggtct agtagagaag tgacagtgga actgatttga cctgtattga
    16921 taccagcaat gatgccattg atattattaa caacggcatc aatttgatct agcaattcat
    16981 caatagcctt cgtgtcttca ttttgaacag ctgtgttcaa agcagatagg cggttttgca
    17041 gattgcttag caagggtttt aattgattta gctgatcaat aattggctgt aatttcgtat
    17101 ttccagcact tgtttgcaat ttagtcagga actcgaccag tttatcgatt gcattttgtt
    17161 gttgagatat tccgtcaata aagccgttca atacattgct gatgtttttc ttttcatcat
    17221 cagtcagctt accatcttca atagctgttc ttacagtatc tattaacgaa cctgtcgttg
    17281 ttgccacttg tccaattgac ttcaatgtaa catctacact gtttgtgatg cttggtatcg
    17341 catctttcag ctgagaggca cctgattttg tttggtttgc tagatcactt gcctgatttt
    17401 ctaaattttt cacatctggc aagatatctt gtacttgttg gatgatcgtc aagccttgtt
    17461 ttgcttcatc gattccttgg ttcattgtct cttggatcga agcaaaatcg ccgtcgatct
    17521 ctgctagttg cttaccggca ttttggatct ctgggatttt ttcttgcaaa tcaaggatga
    17581 tttttgcttg atctttcaac tcaggcattt tgtcattcaa tgcaacgact ttttcaccca
    17641 tctcatctac tttagggatg taatccacga attcatttgc tttattcagt ttttccttga
    17701 tttcaggtag ttgactttgt aattcgagga cttgttttgt gtagccgtcg atcgtgtcca
    17761 aattttcatc tgttgataaa atcatatctt taactttatt gatacttact aggttcgtat
    17821 cgatgtcgta acctatctca ttgaatactt tcaataaagt cgaactggct gttttgataa
    17881 attcatttgt gatttgcgat tggatcgaac ttgctccttt atcggtgatt ttcggtgcaa
    17941 tcgcattgat tttttcattt accgtatatt cgatttttgg tttcttgata tcaccactag
    18001 taaaactcag cagatcttct gagaaatcct ttggcaagta aatacctgcg taatactttc
    18061 cggaacggac tccgtcttct aattgttctt ttgaatcaac gaattgccat cctaattgtt
    18121 tatttttatg aagcgattcg atgacttgct ccccaatggc cacttcttta ccttgaaact
    18181 ctgctggctt gtctgcactg taaacagcaa ttggcagctc tccagtattt ccatatggat
    18241 cccataatgc tttgatgtta aaccacgcat atagcgaagg gatgatcatg atcgcgatga
    18301 tcagaagtgt tgcaatcggg tttttgaaaa tccgtttcca gtcaagcaaa tataatcgaa
    18361 gcgtattttt gattgactgc atagttctcc cccttcttct tttttcagct gaattacttt
    18421 accatgtatt tcatactttt ttcaatcggc ttttttcaaa aaagtcgaat ttttggacat
    18481 atcacggcaa ttgtctcatt gtaatctctt acatattgag aaataattaa ggattacagc
    18541 ctttacttta tcatgaaata aaagggaaag ataattttat gccaaagttt ttatctatta
    18601 tacgtatacg caaaaaagct aaaacatttt ttgtctttgc ccctacaaaa atgtttcagc
    18661 ttttttcgtt ttttattcct gtatttctta tttttcgaat gacaggatga ttttcccagc
    18721 aggaggatgt ccttcaacta gctgatgtgc ttggatgaca tgcttaagtg aagcagacag
    18781 attcattgaa ataaaaacat gaaaggctcc tttttggtag gctttgatta tgccagcaaa
    18841 ggcttctgca tctagatatt cctttcttgg aacaaacgat tcataaaagc cttccttttt
    18901 ttgccgcaaa tctagatctg ggaggtcatt taatgcaaca tacctgccgc ctggtttcat
    18961 gatctggatg ccggtttctc ctttgatact tcctttcgtc gcatcgatta caaggtctgc
    19021 ctgatcagca aattgcagac ctggatttgt tttatcataa gcagcaaatg ctgaagctcc
    19081 taattttttt accttttcct catttttact ggatgcactg gttaagattc ggattccctt
    19141 ttcatggaga agctggatca gacttgaccc gaccgcacct gatgctccta gtatcattac
    19201 agtatctgtc ggctggattt ccgtcaaatg gttgattaga ttataagccg taattcctgg
    19261 cgtgaccatt ccggctgctt cttcccaact cattttatct ggaatttttg ctacttttgc
    19321 tgatggcaac acgacttctt ctccgtaagt cccgccgact gcatggacag ctactcgatc
    19381 acctacatga acagttgtga catctgaacc aatctctgtc acaataccag cgccatcatt
    19441 acctggtaca taagggaatt tcaatgttcg tatctctttc atttctccta aacgcaaggc
    19501 aacatcataa ggattcacac tgaatgcttt tatttctaca cgaacatgtc cagaaccgac
    19561 ttctctggga tgagcatcga tttcttcaaa aacatcttgt gcttctccgt aaccgttaat
    19621 tgcaaatctt cgcatggtca ccatctccct ttttctttaa gtttacgttt atgcctttat
    19681 tttgacaaaa aaaatgtctt ttcgttttgg cgcatgattc aaaacaaaat agttgtcttt
    19741 tttcgcgtaa cgtgttaaat tatttatcta aaagcgtaaa gattacgatt tacgctaata
    19801 aaaaataatt ggaggaattt gttgatggct aaaaaatcaa agattgccaa agcaaaaaaa
    19861 cagcaagaaa cgattgaacg ctatgctgct ttacgccagg aattaaaagc gcaaaaagat
    19921 tacgaaggct tgcgtaaact tcctttagat gctcgtccag aacgattgaa aaatcgtgat
    19981 ttaatcgatg gacgccctcg cggatacatg cgtaaattcg ggatgtcccg tataaaattc
    20041 cgtgaattag cacataaagg tttgattcca ggagtaaaaa aagccagctg gtaatccaag
    20101 tctggctgta atgtaaagga ggagggcgca tggaccactc cttggatcat caaaaaagat
    20161 acgttcgctt cttctagcag aaatgctggt actagaagca gacgtatctt tttttacgca
    20221 cagctggtcc ttttttatat gtaaaggctg catggttact tcgctatatt tatttagtaa
    20281 caggagtcag tgcttcgata cccacactaa ttttcaatgc tcggtcaact tctgccataa
    20341 tgggtcgatc caaatggcag accttttctt tcaatcttga tttatcgatc gttctgatct
    20401 gctccaacag gataacggag tctctttcta ttcctgttcc agtagctttg atcccaatgt
    20461 gagtcggaag cttaggttta gccatttttg ccgttatggc cgcaacaatg atcgtcgggc
    20521 taaaatggtt acctagatta ttttgaataa ccaaaactgg gcgtgttccg ccttgctccg
    20581 atcccacgac tggagaaaga tcagcaaaat aaatatctcc tctttttacc ataggatcct
    20641 ccttattctt gatattctct tggtatccgc tgtcctaatc cgcaagccac ttcatagtgg
    20701 atcgtaccta attgatctgc tatatcctgc atagtgattt ctttgtctgc atttttgccg
    20761 ataagcgtca cttttgttcc gacaggaaat tcttgaggca atcggatcat cagttgatcc
    20821 atacatacac gcccaatcgt ttcacagtag ttcccttcta ccaatagaga aaaaccctgc
    20881 atctttcgtg gccagccatc tgcataaccg atcggtatcg ttccgatcca ctctgcttca
    20941 ggagtgatat atgtttctcc atagccgatt ccttcacctg ctgggagttt tttgacttga
    21001 atcaattcag aaaccagctc aagtgctggc tgcaatggat aaacttccgg tagtgcgtgc
    21061 ccagagggat taagtccgta cattgctact ccataacgaa tcatattgcc gattgtttca
    21121 tcatgccata aagccgttgc actattgctg acatgtacat atcgaggaag ttcgggaagt
    21181 tttttcagca cttctataaa gcgttctttt tgcagattcc aatatgtatc atctgcctga
    21241 tctgctgtag aaaaatgggt aaataaacct tcccattcta atacacgact ttctttgatg
    21301 aatgcagctg cctcttttac ttcttcaggt gtacaaaatc cgatacgacc catcccagtg
    21361 tctgctttta gatgaatcga aagaggcgtc tctagcttac ttttctctaa aacagcagct
    21421 gctgcttgca accattcccg tgtacctgca gttactgata aatcatgagc aacagctaca
    21481 gtcagtgcgt cgtaaggaac catattcaag ataaggatgg gctgagtaaa tccagcttct
    21541 ctcaactcga ctccttcatc taaatttgaa acacaaaagc ccgaagctcc acctgcaact
    21601 gctgcctcag ctgtttcaat agctccatga ccgtatccgt tcgcttttac tactgcaaac
    21661 aattcttttc cttgcggaag tcttgcaact tcatttgcta cattttcagt tatagctttt
    21721 ttatgtatca ctgctttcgt tgggcggtgc caagcgacaa ccatcgtttt tccttctttc
    21781 tagtcttcca aaataatttg tgcaaaagct gtttcatttg tatgagtaat actgacccat
    21841 acattgcctt catgaggtga acgcgtcaca acaggctgac cattttcatt ttttaaaatc
    21901 tcgacatctt gaaaggtaac ttttccgatc ccagttcccc aagctttaga gaaagcctct
    21961 ttgcaagcgt aacgcccgcc taaaaattct acttggcgat gaaaaggcaa ggactggaat
    22021 agtttcattt catcagaggt caagattcgt gcaatgaatt ttggtttttc ttcgatcagc
    22081 cgagtgatcc taggaagttc gactgcatcg atcccaattc cttttatcat atgcttttct
    22141 cttttcaata aatttagaac actctcattc taccaaatta taaccatttt gccacaaata
    22201 agtccacaaa taacaaaaat gaaagtttct cttaaaaaag agaaactttc attttatgtt
    22261 taatcgttat ttgaacggat tacaaatccg cgttttttat cattgtgacg tttaccgcca
    22321 tctttttggc tgttattatt tttgctataa ccgccttttg atttgtttcc acgataatta
    22381 ccgccgtcac gacgattacg gttgtttccg ccaccacgtt tgccattttt attgtatcct
    22441 tttttctgca ttggcaatgg gcgttctggt gtgatcttca ctggcactgc atcagctgga
    22501 tcttttgctg tcgtttttag caaaagtgca actaaatctt gtgcagaata ttcttctaat
    22561 aatttatctg ctgtttgcag gtatttatct agaccatttt ctgcaagttt tgtttcgatt
    22621 tgttcaactg cagcacctaa ttgacctttg aatgcttctt tttcggttgg tggacgtaaa
    22681 gtcgtcatcc gttttttcgt caagttttca atcacatgca agtagtccat ttcatttggt
    22741 gtaacgaaag tcacagacat accgcctttt ccggcacggc ctgtacgtcc gatacggtga
    22801 acgtagcttt ctggatcttg agggatatca tagttgtaca catgagtgac acctgaaata
    22861 tctaatccac gtgcagcaac gtctgtcgct acaaggatat ctaaatgacc gctcttaaat
    22921 gaacgcaata cgctcattcg tttttgttga gacaaatcgc catggattcc ttctgcacgg
    22981 taaccgcgtg cttctaatcc gcgtgccaat tcgtctacac ggcgttttgt acgtccgaag
    23041 acgattgtta attcaggtgt ttgaacatca aacaagcgag tcatgatatc gaatttttcg
    23101 tattcttttg cgcggacata gtattgatcg atcaagtcag ctgtcatttc tttcgctttg
    23161 atcttcacat gatgtggatt tttcatgaat ttaacgccaa tattcttgat agctggcggc
    23221 atcgttgctg agaaaagtaa tgtttgtctt tgatcaggaa cttgtgaaat gattttttcg
    23281 atatcttcca agaagcccat attcaacatt tcatctgctt catccaaaac taatgtttga
    23341 acggttccaa gtttcaatgt gtggcggtta atgtggtcaa gcatacgtcc tggtgtaccc
    23401 acaacgatat gtggacgatc tttcaagcca cggatctgac ggccaatatc tgctccgccg
    23461 taaacggctt gtacacgtac ttttttgtcg cgccctaaac gatataattc ttcttgtgtt
    23521 tggatagcta attcacgtgt tggtgcgata actaaacctt gtaattcgtg acgatctgga
    23581 tcgatttttt ctaacattgg taatccaaat gcagctgttt tccctgtacc tgtctgtgct
    23641 tgtccaataa cgtcttttcc agctaatgct agtggaatcg tttctgcttg gataggcgtt
    23701 gcttcttcaa aaccagcgcg ttcgactgat ttcaacaatt ctggtgataa ttctagttct
    23761 ttaaatttca aatgaatcct cctatatggt tgttaactga ggatgtagct tttttgctac
    23821 cccgtcgatt tgttcggtca aagtgtattg tttctttttg acagtggcca cgcacgtttt
    23881 tatccattct tagcccctgt ggcaccaccg aaaaatcact ttgcgaagtt taggcattag
    23941 aaaagccaaa cgggtcgcgt tcagctttat cattattatg acatctggct tcaacaagtt
    24001 gtttctatgg caaaaccagt ccaatttctc ttcatctttc ggcaataggt tattctaaca
    24061 tagtttttcg ttttctgcaa gtttacagat ttttatttca tttgctgaag ttgttctacc
    24121 acttcaaata agcccatgct gttgctgcct tttagcataa tactatctgt tgcttcaaga
    24181 ttttgtttga tagcatcaat caatttattc ttttcttttt caaaatagaa cacaggtagt
    24241 tgggggtatt tttggttcaa ttcttctttt agtgcatgca tttgttcccc ataaaggaaa
    24301 atcatggcga atttctcagg atcgatatgt tcactcattt gcgcatgcat atttaaagac
    24361 tctttcccta attccagcat atctgctaac acagcgattc tgcgtccttt tgttggcaga
    24421 tttttaaaag tatctaatac cagtcccatg gctgttggat ttgcattgta gacatcgctc
    24481 aaaatagctg caccatttcc tgccgttagc cattcggttc tgttttgagt gatagaaaca
    24541 tgggcgagcc cttggcgtat ctcaggtatg gacagtccaa agaagcgccc gatcgtatac
    24601 gcaatcaaag cattcgttac attataactt ccaggcagcg ggatcgtaaa tgtttctcct
    24661 tctactacaa aatcggtttg tgcttttcct tttgctgtca cttccgcatg aacatcgcca
    24721 tttccaatac cgaatgttgt gactgtttgt actaatgttg ccgttaaagg tgtcagcaac
    24781 ggttcatctg cgggcacaac gagtaatccg tctgatacaa gtccgtcagt gatttccatt
    24841 ttggctttag cgattccttc tcgcgaccca agattttcta tgtgtgcttc accgatcatt
    24901 gtgattgctg cagcttttgg ttgagccata agtgaaagtt ccgtgatttc attggcgtga
    24961 tccatgccca tttccaaaac gatcttttct gtatcttggg gcatgtgcaa aatcgtgtaa
    25021 ggaagaccaa tattattgtt gtaatttcct tgcgtcttat atgttttgta tttttcagac
    25081 aagacagacg cggttagatc tttcgtggtg gttttaccat tgcttcctgt aattgcaacg
    25141 acttctgcgc ctactttttc caagtaatag gttgccaatt gctgcatggc tttcaaagga
    25201 tctgctacag gaataactgg aaaatttttt ggagcttgtt caacaggcaa gctccaaaat
    25261 gaagcagatg ctccgtttgt gatcgcttgc tggataaatt catgtccatc tcgcgtacca
    25321 gcaagaggaa cgaataaact tccttcggtt atcttccggc tgtcaaattc gacattggta
    25381 atctttgtca gtgcagagca ttcgctgccc acggcttgtg caatttcttg tattgttaga
    25441 ttcattgaga tctctcctaa aaaaggttgt gaaaaaagcg tttggcctaa acggcgggtt
    25501 cgcatgactc tcacttccaa gtaaatttct ctattcgtat ttagaagcta gaatgaacgc
    25561 ggttacgcaa tcactccagc ctcttcttta tttctcttta ttcatttttt tcaaaaaaag
    25621 attgacgctg atggtatcga ttcattccta actggatcag ttcttcaatc aaatcaccgt
    25681 atttcaagcc catattttcc cataagagtg ggtacatact gaactccgta aatcctggca
    25741 tagagtttaa ttcattcagg aataattcat ttttatttgt caaaaagaaa tcgcaccggc
    25801 tcaatccgct tccacctaac atcgtgtaag ctaacttcgc gtactcttgc gctttttgat
    25861 aaacttcttc tggcacttcg gctggaatct gcatttcgat tttattattg atatattttg
    25921 cttcataatc atagaatgct acgtctttta cgacttcacc aggcaaagtc gtccgaacgt
    25981 cttcatttcc taatacagca acttcgattt cgcgcgcttc aattccttgt tcaacgattg
    26041 ctcgagaatc atactgatag gctgttgcta aagcattttg cagctcttct cggttttctg
    26101 cttttgtaat gccgacacta gaacccatat tcgccggttt gacaaacatc ggataaagca
    26161 aagaaccttc acattgatca aatacttttt taggattttc tttccattga ttcttaagta
    26221 ctggtacata aggaacttgc ggcacaccag cagcttgtaa aatatacttg gtcatgattt
    26281 tatccatggc acatgcactg gtcaatacgc ctgcgccgac ataaggcata ttcaatgtct
    26341 ctaagaagcc ttggatcgtt ccatcttccc cgtttggccc atgtaaaact ggaaaaacga
    26401 tggctccttc ttctttgatt tcgcccggat tgatcacttt tcctgtaaag ccttcctctg
    26461 tctgtccact tgggtcccat gaaagatgca agacatcttt gctggcaggt ttttctgtta
    26521 gtaatggacc tttgacccat tgtccttctt ttgtaataaa tacgagttga acttggtaat
    26581 aattataata aatggcattt aaaactgaaa atgcggaaag aatggacact tcatgctctg
    26641 cgctgcgtcc gccatatagt aaagtaatct tcaaaagatt gtcctcctgt attctaaatc
    26701 gttcagtgat ttactctcat attttagcac aaaacaaaaa taaacgaata acttatttat
    26761 aaaaaaacga gccagcttct aaaagaacga gcgtcattta ttttttgtca gctagattat
    26821 ttgtcccgtt ttttataaaa agttatgata aagaaaagga gggatgatga tggcgcaaac
    26881 aaatccacgc agccgcttgg cagaaatcat tcgagtgttc atcaattaca atgtagtgcc
    26941 aaactttgta cagcaaaaaa atcccgaaca ggtaaaaaaa gcctttgaag aactaggccc
    27001 caccttcatc aagataggac agatgctttc tgttcgagaa gatttgcttt cttctgcctt
    27061 cactcaaaca ttcaaaacat tacaagatag tgtacctagc gacacatttt caacggtcaa
    27121 aaagacgatc gaaacagaac tttctctttc attatcagat atattcgatg atttttcaaa
    27181 atcgcctttt gcctctgctt ctatggggca ggctcatcgt gccaagctga agaacggtga
    27241 cgcggtagtc gttaagatcc aacaccctaa catcgccgaa gagatccgac ttgatcttca
    27301 actatttgaa cgagcgatcc ctttgatcaa atacattcct gaaactagcg tagttgatct
    27361 aaaaggggta ctccaagaag taaaacgttc cttgatcaat gaaatggact tcttaaaaga
    27421 aagtcaaaat ggcgaacaat tctatcaaaa gaacaatggc tggaaagaga tccgcagccc
    27481 caagatttat gacgcttttt gttcgaaaaa agtgatcgtg atggaagaaa tgtctggtaa
    27541 aaacttgaat catttaatga acgccgaaaa caaaactgaa acttttatca ctggaattca
    27601 aaacaagcag ctgaaacaag aagttgcaaa gcttttagtt gagaacttta tgaaacaggt
    27661 gtttgatgat ggttttttcc atgctgatcc tcatccaggg aatctgctat tccatgtact
    27721 aacaaaagaa gaacagacac aagctagccg aaaaactgaa acagtccatg aaaaagagtt
    27781 cggttccttt gcttttcgag cctccacttc tgcagaagac cctgttgcgc catataccat
    27841 caactacatc gattttggta tgatggggca tctttctgca ggacttaggc aaaaactcac
    27901 acaagccgtt ttagcgctgt atacgaaaga tgcttatcga atcgaaaaag cggtccttcg
    27961 actttgtcag caagaaggct cgtttgatga aagtcggttc caccaagaac tgacaagttt
    28021 tcttgaacag tattatgatt ctcctattga tgaaatcaac ttgcaagaag tattcacaca
    28081 tgtcgtgacg atttgtcatc aaaataatct gcagttcgat cgtgatatca cgcttttgct
    28141 gaaagcattt gggacattag aaggggtgat ccgcgtcctc gatcctgaag tttccctcat
    28201 ggaagtcgct tcgccttttg ctcaacatta ttttctcact catctggatg tagaagatac
    28261 attgaaacaa agcggattag acttattgga agggatgaag gctgcgccta agatccctca
    28321 acagctccac catctattag aaatgtggac ttcgggacaa ggaaaagtca atttggaatt
    28381 gaaaaagcaa gacaaattat tgagcaggat cgaaagtatg atcaatcggc ttgttttcgg
    28441 tatgatttta gctgcattga tcgtagggtc ctccctgctt gtccaagcag cacctgtaga
    28501 aaacgcagag gtcgtttcct tattagggat atttacgtat gctattgccg cattcgtcat
    28561 tatttttttg gcaatcgatg cattgatcca aatgtataag aagaggaaaa agtagaaaaa
    28621 ggttgtgaca gaaagaatca gcttcaagaa ataagaaggg gttttacata aattgtttct
    28681 caaatttttg tgaaaattcg gcttattttt cgaagaggct acttctgaaa caccgagaat
    28741 aaaaaatgtg aaaaagctgt tctgcaccca gtaataagaa caatcaaaaa gggaacatgg
    28801 aacacattta tcctgtttcc ctgttccctt tttgatgtat tcgagcataa ataaaatacc
    28861 ttcgttacct actatcttct gtacttggta aaccttagtc aattgccttt tttaacgctt
    28921 gacgaaaaga catatgatct ttctccgtaa agggttttgg accctttgtc cttatgccag
    28981 cttttcttaa ctttccacta tgataacgtt gttctagaag cgtgccgata ttcgcttcgt
    29041 caaattcttt tccagagtga tggaacactc gcacaccctt cggcaataca agtgaagcta
    29101 aaccataatc ttgcgtgatc aaaaagtcat tcttcttaac cataccgacg attttataat
    29161 ccgcactatc agctccgcgg tctacgtaga cgatttggac atgattagcg tattctttgg
    29221 tcgtataatg gtccacactg gtcacgataa ccacgtcaag accataggat tctcctaatt
    29281 ttatgacatc ttcttttact ggagaaccat ctccatcaat gaatagcctc attctttttc
    29341 gccttcttcc ataaatgata aacagttact aatccgctca gtaaaagacc aacattgatc
    29401 cattttgcca agataagcag ttgtacactt tctatcggca ctgattcatg gttcagcgtg
    29461 atcaataata gatggattgg ctctcccatt tcttttgcta aaacaaacaa aagcgtcatc
    29521 aataaaaact ggtggatcac actcccttta ttttgagtag tatacttgac aacgatccgg
    29581 cgatccattg gaatagctgt atcagtttga taatattcct tatttagcca gctgcctgtt
    29641 actacgtgcc agaaactaag cagaaagaca cctatcgcaa aaccatttgg aatcaagagg
    29701 accaataaag aagaactcag tccaagcaat tgcatagcca atggagacca tgttaaacgt
    29761 ttcaaaatcc atggtccgac tatttttgct gcaatcaagc ctgctgcata aggcaaatac
    29821 ataaaaatcg aaagtttttc taatccgtac agtatgccaa cataaaaact gctaaataaa
    29881 aaaaggaaat tccctagcat tccatttaga aaagtaaaca gatttagcca gcttggcaac
    29941 ttccattcct tcttctggtg tcctaaaaca aacacgaaca agatgaacag cagcaagaaa
    30001 ccataaatag caagatcaaa aagcctttca tcgaataaca atcgggcatt tctcaaaaga
    30061 aacaataagc caaagaatat caaaaataaa acaagctctc gcgtagctac cagatttctt
    30121 ttgatgtctt tgaaatccag ttcgtaacgc ggataatgac taaccgtatg gtaggccatg
    30181 acgtaaaaaa gtgtataaat agtaaagctt gctgcaatct gagtcggtgc aggcaactca
    30241 atcgcttgat acaggagaaa tagccagata gctgcaaata agtacttttt cccatccata
    30301 ttgataaaac cttgcgtctt ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat
    30361 gctgcactca gccctagcaa aacacttgaa aaataaaata aaggaaaata aaatacgcct
    30421 aatattccaa gcaatgaacc agcaccacca atcagcagag cgatcatcag caacgtgtaa
    30481 ctatctagac caaatcgtag actgttgact aaaaatagtc cagtcatccg aaaggcataa
    30541 aacagcgtaa atggcaaaat cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa
    30601 agataaggaa taaaaataac cgtgttactt aagataaagg gcgttgtttg tgtaaaatta
    30661 cgcccaaatc gtttgagttt ttcgaacatg acaatggctc cttaaacagt cttataacta
    30721 atctgctctt cgattttagc attaaagggg aaataaacca attttacaga atatattctt
    30781 gtaaaaaaaa atacagaaag aagcataagc ttctttctgc atttttacgg tacttgatta
    30841 ctcaagcaga caagcccttc tccacaaaag tcttgcccga cacccctgca tccggcaggt
    30901 gataaagata gaaatcaatt atcctgtgcc tccaaaatca attaggagct ctgtaaacca
    30961 gagtccttct cgacccgtcg cataaattta ctttaacaca gttcctatgt ttatgcgaga
    31021 gcttttttca ttttataatc gaccatttga aatccttgac cgatatctga aataagttct
    31081 tctgtaatct caaatccttc atgcttataa acctcgattg cttgatgatt cccctgattc
    31141 actcgcaaaa agatctctct ttcctttgtt tgcgcagcaa gttcttcata ccagcgaaaa
    31201 actgaagaag tcaatccttg accacgtact ttatttagca aatataattt acttaaataa
    31261 atttttccat ttatctcttc ataagcagtg taaccgacag tcttttcttc gctttttaat
    31321 aaaaagtagg acacaccttc tgtcagttct ttttgaatct gtttttttga ctgataagtt
    31381 ttcagcatgt aatccacttg tttttgacca atgatcggca caaaaacttc cggccatact
    31441 tcttgtatga ctgccatcag ctcatccagt tcattttctg ttacttttat tttttccata
    31501 cacccacgtc cttttttaca ttataaatga ccttgcgctt gatggaataa aaagagcatg
    31561 atcatgctat acacacccac cgaccacggt ttcaagaaaa tcacaatctg cataaattcc
    31621 ctaaaagata atttcgtcaa accagctact agacagatca agtcgtctgg cgcaaaggga
    31681 agcagcaagg taacaactag tactttttta atatctttcg tttttcttgt cagcaattca
    31741 tcaaatttct gatacttttt tggcgataaa ataagctgga caaaggctac gccatagtaa
    31801 cggacaagca aaaatccgat tgcttcccca atcactaggc caatacaact atataaaagc
    31861 ccaaatccat tgccaaacat cagcatgccc gctactgagg agatccctcc cggcaaaata
    31921 ggaatgatca cttggatcac ttgcaacaga acaaatatca tcactgcata cttgccaaat
    31981 tgctggataa acgcttgcaa agacgtgctc gatcgaaaaa tcccatgatg ataagcataa
    32041 agaatcaaca caaagaaaaa aaacagacca ataatgggca tgatctgaag acatttttgt
    32101 aaaactgatc cttttttaga catctatatt ccccacatct tttttcattt attaaaactg
    32161 cctccttatg aactgaaccc caaaagttga actatttaat ggactgtttc cgatattcta
    32221 ctggagatag tccatttaac tttagtttta tccttttgtt attataccac ctaatatact
    32281 catttaattt actttgaaat atctcaattg atcggaattt ttctcgataa aaaaactctg
    32341 atttaagcac accaaaaaaa ttttctatta cagaattatc taagcaattt ccttttctag
    32401 acatgctttg aataatgtta ttctctttta attttttttg atattgtggc atctgatact
    32461 gccatccttg atctgaatgt agaatcagtg aaccctcagt tccctttttt ttaattgctt
    32521 gttgaagcat ttcttcaatc agtttatatg ttggactagt tgatatacta taactaatta
    32581 tttctccgtt aaataaatca agtataggag atagatagat ttttcttcct tttatcttga
    32641 attcagtgac gtctgtcacc cattttttat tgggtgtatc tactgaaaaa tttcgtttta
    32701 acacattctt ggcaattttt cctactgttc ctttataaga tttatatcgc tttattcgga
    32761 tttgacaggt aagtcccatt tgggacatta attttctaac tgttttatga ttgattgtat
    32821 atcctttcat ctttaacgct aaagtgactc tacgataacc ataagagttt cttgattctt
    32881 ttacaatcgc tgtaatttct tgctttatct tgctatattt atctggctta tctaattttt
    32941 ttacccagta ataataagtt gacttcgcta attgtgcaat tgaaagtaat agattcaatt
    33001 taaattcttt tttgagctga aggattgttt taaccttgat ttcttcttgc tcaaatcttg
    33061 ttcttgaatc aaggtttct
//
LOCUS       DAZKOU010000023        32322 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000023 DAZKOU010000000
VERSION     DAZKOU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.023"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..801
                     /gene="truA"
                     /locus_tag="ACG827_001033"
     CDS             55..801
                     /gene="truA"
                     /locus_tag="ACG827_001033"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0483191.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(1256..3925)
                     /locus_tag="ACG827_001034"
     CDS             complement(1256..3925)
                     /locus_tag="ACG827_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0483192.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(4163..4351)
                     /gene="rpmB"
                     /locus_tag="ACG827_001035"
     CDS             complement(4163..4351)
                     /gene="rpmB"
                     /locus_tag="ACG827_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0483193.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            4621..4983
                     /locus_tag="ACG827_001036"
     CDS             4621..4983
                     /locus_tag="ACG827_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0483194.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5035..6717
                     /locus_tag="ACG827_001037"
     CDS             5035..6717
                     /locus_tag="ACG827_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0483195.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            6834..8870
                     /gene="recG"
                     /locus_tag="ACG827_001038"
     CDS             6834..8870
                     /gene="recG"
                     /locus_tag="ACG827_001038"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0483196.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            8919..9920
                     /gene="plsX"
                     /locus_tag="ACG827_001039"
     CDS             8919..9920
                     /gene="plsX"
                     /locus_tag="ACG827_001039"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0483197.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            10042..10284
                     /gene="acpP"
                     /locus_tag="ACG827_001040"
     CDS             10042..10284
                     /gene="acpP"
                     /locus_tag="ACG827_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0483198.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            10552..11556
                     /locus_tag="ACG827_001041"
     CDS             10552..11556
                     /locus_tag="ACG827_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0483199.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            11557..12501
                     /locus_tag="ACG827_001042"
     CDS             11557..12501
                     /locus_tag="ACG827_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0483200.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            12501..13463
                     /gene="opp4B"
                     /locus_tag="ACG827_001043"
     CDS             12501..13463
                     /gene="opp4B"
                     /locus_tag="ACG827_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0483201.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            13490..14404
                     /locus_tag="ACG827_001044"
     CDS             13490..14404
                     /locus_tag="ACG827_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0483202.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            14430..16211
                     /locus_tag="ACG827_001045"
     CDS             14430..16211
                     /locus_tag="ACG827_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0483203.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            16559..17245
                     /gene="rnc"
                     /locus_tag="ACG827_001046"
     CDS             16559..17245
                     /gene="rnc"
                     /locus_tag="ACG827_001046"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0483204.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            17265..20846
                     /gene="smc"
                     /locus_tag="ACG827_001047"
     CDS             17265..20846
                     /gene="smc"
                     /locus_tag="ACG827_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0483205.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            20855..21664
                     /locus_tag="ACG827_001048"
     CDS             20855..21664
                     /locus_tag="ACG827_001048"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0483206.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            21675..22673
                     /gene="ftsY"
                     /locus_tag="ACG827_001049"
     CDS             21675..22673
                     /gene="ftsY"
                     /locus_tag="ACG827_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0483207.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            22860..25127
                     /gene="gshAB"
                     /locus_tag="ACG827_001050"
     CDS             22860..25127
                     /gene="gshAB"
                     /locus_tag="ACG827_001050"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0483208.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(25216..25668)
                     /locus_tag="ACG827_001051"
     CDS             complement(25216..25668)
                     /locus_tag="ACG827_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0483209.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            25818..26768
                     /locus_tag="ACG827_001052"
     CDS             25818..26768
                     /locus_tag="ACG827_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0483210.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            26761..27720
                     /locus_tag="ACG827_001053"
     CDS             26761..27720
                     /locus_tag="ACG827_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0483211.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            27717..28472
                     /locus_tag="ACG827_001054"
     CDS             27717..28472
                     /locus_tag="ACG827_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0483212.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            28495..29448
                     /locus_tag="ACG827_001055"
     CDS             28495..29448
                     /locus_tag="ACG827_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0483213.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(30092..30388)
                     /locus_tag="ACG827_001056"
     CDS             complement(30092..30388)
                     /locus_tag="ACG827_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0483214.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            30743..31099
                     /locus_tag="ACG827_001057"
     CDS             30743..31099
                     /locus_tag="ACG827_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0483215.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            31109..32008
                     /locus_tag="ACG827_001058"
     CDS             31109..32008
                     /locus_tag="ACG827_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0483216.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        10708 a         5732 c         6906 g         8976 t
ORIGIN      
        1 ttatagaaaa atcattattc cagataacct tttaggaaca aaggagagaa aaacatggta
       61 cgttacaaag caatcattgc gtacgacgga acaaatttta atggattcca aaaacagcca
      121 aatgggcgta ctgttcaaga agaagtagaa aaaacattac aaaaaatggc aaatggaaaa
      181 gaaatcacgg tctttggttc gggaaggaca gatgccggag tacatgcgat cgggcaagta
      241 atccattttg attatccaga ggagcgtccg ttagaacgaa tgcgtttcgc gctagatacc
      301 cagtcaccag aggatattgc tgtaagacaa gtagaaattg tttcagaaga cttccacgca
      361 agatatttgg tcaaagaaaa gacgtatcaa tttcgagtgg atatagggaa accacgcagt
      421 ccttttcgac gccattatgc aagttatttt ccttacccac ttgatttgga aaaaattcag
      481 cgggcgcttc ctgatttatt agggactcac gatttcacct ctttttgtgc atcaggaagc
      541 tcgatagagg ataaaatacg tacgatctac gaggctaaga tggaggtaaa tgaaacaaaa
      601 gacgaattgt tgtttacatt taggggaaat ggcttcttgt ataagatgat acggatctta
      661 gtaggaacgc ttttgaaaat tggcaatggc cgattgcctg aagatagtat cccagatatt
      721 attgcgaaaa aagatcgtaa tgcagcagga cctacagcac accctgaagg actttatttg
      781 tatgaagtga aatatgaata agaggttatg atagaagtgg tcagcttcaa gaaataagaa
      841 ggtatttacg aaaattactt ctcaaaattt tgcaaaattt cggcttattt tcgaagaagc
      901 tacttctgga acaccgttta aggaagaggt cgtgaaatca gcgttttgac ctgagtatta
      961 gagaaacaaa cgggaaaata gctcctcata ttttgccgtt tgttgagcta ataccgaagg
     1021 tcagctgatt gaacaccgtt taagaaagag gtcgtgagcc tgtcatttaa ctctgagtca
     1081 caaagagcaa tttcaaaaaa cagcttctca tctttgatga atatcgtgac ttgtggacga
     1141 ggcgttgata agtgaatcat ctttttgtta cctatttttt ctgtaaccaa aaaacgatgg
     1201 aacgttgttt tgattgccct tggtgcggac agtctgaaca ggttccatcg tttctttatt
     1261 ttctacgtct tcttctgtgt gtgtttcgtg gttgttcttc tattgctgga tcaatcgtgc
     1321 cataaaaggg gcgattgcgg tcaaggtacc agacataaag aataaacaat gcaatacata
     1381 ggacaaaaag gattgcacct atcgtaaatc cttcaggcaa gtaactgaaa gtgacagtat
     1441 gtttacctgc tggaactttt atcatcaaga atgcgtcttt gaaattgctg accttgactg
     1501 gtttcccatc aattttagct ttccatcctt tgtcataagg aatagttgtc accaacatgg
     1561 aatcttcagc ggtatttaca gtcccagaag cttttctgcc agtggcttcc atttcgacac
     1621 ctttttcttt gatctgattc attgcggctt gataggctac aacatctaga ccaacgactt
     1681 taggctccat gaaacttatt tctttcgtcc cataaaaact tgcagtaaat gtcactgttg
     1741 tctcttgagg ataatagcca agatcatagt attgaccaga aatgttgatt tgcgtttttc
     1801 gcgatgtacc attgacagtg atcgtcgcac tagaactttc taattgcgca aaattggttg
     1861 ggaacaaact taagtaagct tgtgtatttg caggcacatg gacttcccaa gtgagctctt
     1921 ttggctggtt atctgctgtt tctgagtaag tgacgccagt cgttgtcttt gtgatcgtcg
     1981 tattcaactg attcttcaaa gtaggtgtat agaatgtaaa atactgttca tttagaccag
     2041 acaatgtatt gatcaagttt gtctgactgg tcaaattatc tgtcactggc tgctcaacat
     2101 cattaacgga caacggtgca gtaaatgcta gtggcaatgc attgacattc tgatacagtg
     2161 tatagtcgcc gctttgacca gcttggcgga agccatattt actgaacgtg ctttcgtttt
     2221 tagaaatgtt atatttgatt cctgtaaacc catccatcag caaagtatta ttgttataac
     2281 ggatattcaa atttgtccct cttgaacgga aacctaattt atccaggtaa gaggaagaat
     2341 tacgatttct tatagaagaa aacaaactga taccactgta accatagttg atcgaatcgt
     2401 ttgcagatac tggatccaga ttttccaagc ggtaaaacga gtcggaatct ttttttgttt
     2461 catttacaag agctttcaag tcaggataag gatcagtata taaactgcgg gaagcataat
     2521 tccaatcatc aagaatgcca tggatcatac tgttcgtatt gaccagtgct tcagcagaca
     2581 tgatcaataa taacaatata ctcagatgat tgaatgaaag ttttttatgc tgataaaatc
     2641 ccacagcaaa agcataaatc cctaagaaaa ccactgtcaa tacaaaggaa gtcatcgtca
     2701 cataggtgta gcttccttca ggtttgatgc cccatgccac cgcaaagaga gcagctacga
     2761 ccaaaatgat tcctaacaac agcccacgat cgtcttgttc tatcttttcc catccttttc
     2821 ctgcaagcag gagcactaag aaagagaaca gataggcata acggaataaa aacatattcg
     2881 gtgcatgcat accatgccag aacaaattca atggaacaag ataaaaactt gcgataagta
     2941 tggcgaaaag actgccgtac aaaaatttat ttttcaacgg tactttccga ctggcaaaat
     3001 aaaacagaca gaggactaat ggtagcagac ctgcgtaaac gaacggaatc gatccgtatt
     3061 ttgtcgtgtc ataaacacca atcatatttt tcatgattag atccagataa gaagtcgctt
     3121 ctgttttcaa cgtagtgatc tgactcagtg cctcgccatt tgaccgaaga tccagcactg
     3181 ctggcaatac aatgatcatt gaagcgccgc ctgccagtaa agaagtgata ccataaggaa
     3241 caatcgtttt tttatattct gaccagtttg tcaatagacg ggcgataaaa tataagaaag
     3301 aaaatacgcc aatcataaaa cccatataaa aactggataa aaacaagagt aaataactga
     3361 taaataaaag tttcggccgt ttaagatcca taacacgatg aatccccaaa atcactaatg
     3421 gaagataaaa aaatgcatcc agccacatga tgatctccga atgtgcagtg ataaatgaga
     3481 tcaatgcgta actgacgcta agtgcaacat gcccccagcg aggaatcttg aacgtacgtg
     3541 caagaaacca aaaagccaat ccggcacagc cgaccttaag caaagtcaga acatacagtg
     3601 catctggcat catctggttt ggaaaaaaga acaccagtgg cgtgaagatc ccgcctagat
     3661 agtaagaaat taaagataaa taattcaatc ctagagaagc attccatgta taaaaaatac
     3721 tttgctttcc aagaagaaca tttcggaagc tggcatgaaa attagaaaac tgtgaaaagg
     3781 catcacttgc caaaatactt ctgctgcttc caggatagat tccgatactt agataaacaa
     3841 gtgctaaaat caaaaacgga atcaggaaac tagctgctgc gtagcgtccg ttttctctaa
     3901 agaatgactt taaatttttt ttcatattta ttttccacca aatttctatg aagttgtatt
     3961 cagtttatca tatccttgtt tttctctgct taaaaagagt ctagttttca caaaaaaata
     4021 aaatttagaa taaataactt cataatttgt tcgtataaca aaaataaacg caaaaaaagg
     4081 gtaggaatca aaagtcccac ccttaaaaat ttttattaaa taaaaaaccg ttcactatct
     4141 gaacggtcca agtatcattc aattaaacgc gttcaacttt tcctgatttc aaagcacgag
     4201 ttgacaccca aactttttta ggttttccgt cgaccataac gcgaactttt tgtaagttag
     4261 gtttaacagt acgttttgtt gagttcatag cgtgtgaacg gttgtttcca ctttttgtct
     4321 tgcgacctgt aaagtaacaa acttttgcca tggtaatttc ctcctttgcc ttgatttgga
     4381 gcaatagaat aattcagctc ataccagact aacatatcat atagaatggg aatttgcaag
     4441 tacattttca tagatacaat tgacaattga gaagaataat tgtaaattaa acaaggttat
     4501 acctgaaatt ttgcaaggtt tttgagtact ttgggtaact gccttttatt tcaagttgcg
     4561 ctatgataaa atatgataga agaaaaatgg gagtttttta cccgttaagg aggctcattc
     4621 atggctgtaa aaattcaaac gtcagctgga accattgaaa ttaccaatga tgtaattgca
     4681 acagttgttg gtggcgccgc gacagatgtg ttcggtgttg taggaatggc cagcaaaaat
     4741 caaattaaag ataatattac tgagattttg cgtaaagaaa attactcaag aggagtagtt
     4801 gtccgccaag aagataatgg tattgctgta gatgtttata cgattatcag ctatggtacg
     4861 aaaatctctg aagtttcccg caacgtccaa gaaaaagtga aatacaatct agaggcatta
     4921 ttaggcgtaa ctgccaattc agttaatgtc tttgtacaag gtgttcgtgt actgccagac
     4981 taggcaggag actggctggt aactcagcag tcgatctgaa ggaggattat ctaggtgagt
     5041 gtaacagaaa taagtgcaag ccagttccag gaaatggtcc aggcaggtgc gaatcgtctg
     5101 caagtaaatg cagaatacgt caattcatta aatgtattcc ctgtgccaga tggcgatacg
     5161 ggaacaaata tgaatctatc tatgactagt ggagctaagg cagtagtaga ttcaacctca
     5221 gaaaaagtag gagaattagc agcaatgctt tcaaaaggat tgctgatggg tgcgcgcgga
     5281 aattccggcg ttattttgtc tcaattgttt cgtggatttt ctaagcaaat tccagatgtt
     5341 gtctcattaa atgcttctga tcttgctgca gccttcactc atggagttga aactgcgtat
     5401 aaagcagtaa tgaaaccagt agaaggaacg attttgacag tagctagaga agcagcgaaa
     5461 gctggagaaa agaaagcaaa aagtactgat gatgtcgttg aagtaatgac agctgttgta
     5521 aaaggagcaa agcgtgcttt agctaaaact ccagacttgc ttccagtcct aaaagaagtc
     5581 ggtgtcgtag acagtggagg gcaaggactg ctgtttgttt atgagggctt tttaagtgca
     5641 ttgaatggag aattccaagc ggatgaaaca tatgaacctt ctccagcaga aatggatgat
     5701 atggtcaatg cagaacatca tcgcagtgtc caaggtcaaa tggcgacaga agatatccat
     5761 tttggttact gtacagaaat catggtaaaa atcggagaag gacctacagt cgatagtaca
     5821 ttcgattacg aagaattccg taactattta gatggtatag gagattcttt acttgtggtc
     5881 aatgatgacg agatcatcaa agtccacgtg catacagaaa acccaggaga agtcttgaat
     5941 tatggacaaa aattcggttc attagtcaaa atcaaagtcg acaacatgcg cttgcagcat
     6001 gagacgattt tggaacacga tgaagaagtg aacgagtttg cgaaaaaaga aataccacgt
     6061 atcccgtatg gtatcgtagc gatcgctgct ggtaaaggcg tccaagagct gtttgaaagt
     6121 ttaggcgcta attatgttat cagtggtgga caaacaatga atccaagtac ggaagacatt
     6181 gttaaagcga ttcaagaagt aaacgcagat aaagtgattg tcttgccaaa caataagaac
     6241 atcttcatgg cagctgatca agcagcagaa gtagcagata tcccagtagc agttgttcct
     6301 tcaagaacga tttcccaagg attgacagct atgctttcat tcaatgatca agcttcttta
     6361 gaagaaaaca aagaaacgat gacagaaatg ttgtctagcg ttgtgagcgg tcaagtcact
     6421 cacgcgatca gagatacctc aattgacggt gtagagatca aagaaggaga ctttttgggc
     6481 atgatcgatg gaaaaatcct gatctctaac ccagatatca cagcaacagc gttaagtaca
     6541 ctagaacaaa tgatcaatga ggacacagag atcgtaacga tcttggcagg tgatgaagga
     6601 acagttgaac aagcaaatga atttgctgag aaactaacag aaaaacatcc tgatttggaa
     6661 atcgagatcc accaaggaga tcagcctgtc tatccttatc tattttctgc tgaataattt
     6721 ctgtgtcaga atatctttct aacacagtat tcattcataa aaggccttcg gttagtgatc
     6781 aggtatgatc gctgaccaag tccttttttt gttaaaggag ggaggaaaaa tttatgcgtt
     6841 ctttagatga tccaatcact atgctggcag gagtcggaga aaaaagagca gctagtcttg
     6901 ccagcttggg catccaaacg attgaagact tactgactta ttatcctttt cgctacgaag
     6961 atattcagga aagaaatcta aacgagatcc aggatcaaga aaaggttact ttgaaaggaa
     7021 tcgtagtctc gcctcctgtg atgaatcgat ttggttataa aaaaagccga ttgcaatttc
     7081 gtatgatgca agaacatgat gtgttcaacg tttctttttt taatcaacct tatctaaaag
     7141 aaaaagtcat tttatcggag gaaatcgccg tatacggtaa atgggatgca aagcgtaaag
     7201 ccttgaacgg gatgaagatt ttaggttctc aaacaatgga tgacttttcg ccgatatatc
     7261 acgtcaacaa atcaattcgt caaacgacat tagttgatct aatcaaaaaa gggtttcaag
     7321 aatacggcac attgattgaa gaaaatctgc caaacaacct tgtggagaaa tatcgactgc
     7381 ttgaccgagc ttctgctgta agaagtatgc attttccaaa agaccacgaa gaaaaccatc
     7441 aagccaaacg acgtgtcgtt tttgaagagt tctttttatt tcaaatgcgt cttcaaggat
     7501 tgaaaaaagc agaaaaagca gaaacgaatg gtttggagat cctttatgat gtccaaaaat
     7561 tgaaacaatt tactcaaaag ctaccttttg aactgacaaa tgcacaaaaa cgtgtaacga
     7621 atgaaatctg tcgggatctt cgcagtccgc agcatatgca gcgacttttg cagggagatg
     7681 taggtagcgg aaaaacagtg gttgccgcga ttgcgttata cgctaccgtt acagcaggtt
     7741 tccaaggtgc gttgatggtg cctacagaaa tcttggcaca acaacatatg gaaagcttgc
     7801 aacagctgtt tgatcccaat gaagtaacca tcgcattgat tacggggtca acgaaagcaa
     7861 aagaacgacg tgagctcctt gaaaaactag agcaagggga aattaacgtc gtgatcggca
     7921 cacatgcatt gatccaagat ggagtcgaat ttcagaattt aggtctagtt attacggatg
     7981 agcagcaccg ttttggcgtg aaccagcgga aagtacttcg tgaaaaaggc tggcgaccag
     8041 atgtcttatt tatgacagcg actccgattc ctagaacatt agctattaca gcatatggtg
     8101 agatggatgt atctatcatt gatgaacttc cagctggacg tatcccgatc gaaacacgct
     8161 gggtaagaac acctcaattg gacagtgtac tggattggac gaaaaaagaa ctggcaagag
     8221 gacatcaaat gtatgtcatc tgtccgctga ttgaagaatc agaagcatta gatgtaaaaa
     8281 atgcagtgga aatctatgaa aaacttcgtg aactatttgc accaaaatat gaagttggct
     8341 tgttacatgg taaaatgaag aatcaggaaa aagaagtaat catggaagcc ttcaaagaca
     8401 accagatgca gatattagtt tcgactactg taattgaagt aggggtaaac gtgccgaacg
     8461 cgaccgtcat gctgatcatg gatgcggatc gatttggatt ggcacagctg caccaacttc
     8521 gtggacgtgt tggacgcgga agtgatgctt cctactgtat cttagttgct aatcctaaaa
     8581 atgaactagg tgtggaacgg atgaaaatca tgacagagac caccaatggt tttgtactaa
     8641 gcgaaaaaga tctggaatta cgtggtcctg gtgaagtatt tggcttccgt caatctggtc
     8701 tgcctcaatt tgcgattgct gacttggtga cggatggaaa tgttttggag gtagccagag
     8761 aagaggctgc agccctttgg caaataaagg attggcagat gcttcctgag tatcagcctt
     8821 tagcgcaaag cctccaaata gaaaatcagg ataatcgtta tttcgattag cagactgtgc
     8881 tatactttat actgatgacg gaaaaaggag tggaaacaat gaaaattgcc gtagatgcaa
     8941 tgggcggaga tcacgcacct caagcaattg tagaaggcgt ggctttagcg cagcaagatt
     9001 ttccagaagt agaattctta ctttacggaa aagaagcaga aatcaagaaa tacttaacaa
     9061 atgaaaaaaa catcacgatt atccatacag acgaaaaaat caatagtgat gatgagccag
     9121 taaaagcaat tcgacgaaaa aagactgcat caatggtcct tgctgcacaa gcagtgaaaa
     9181 acggtgaagc agatgctgta ttttcagcag ggaatacagg cgcgctacta gcagcagggt
     9241 tgttcatcgt aggacgtatc aaaaatatcg aacgccctgg cttgatgtct accttgccag
     9301 tagtagggaa agaaggtgcc ggtttcgata tgctggatct tggcgctaac gcagaaaaca
     9361 aaccagaaca tttgttgcaa tacggtattt taggttcttt ttatgcacgt aaagtccgag
     9421 gtatcggacg tcctcgtgtc gcactcttaa acaacggaac agaagcaaca aaaggtagtg
     9481 aagtgacgaa aaaagcctat gaactccttc agaatgaaac aagcttgaat tttattggaa
     9541 acgtagaagc tcgtgagcta ctcaatggtg tggctgatgt agtggtcaca gatggattta
     9601 caggcaacgc tgttttgaaa tctattgaag gaacagcaat gaatatgatg aatctattaa
     9661 aatctgctat tctaaatgaa gggatcaaag gaaaaatggg cgctatgctt ttaaaagacg
     9721 gcttacgttc tttgaaagca gaaatggatt attccaaaca tggcggtgcc gttttgtttg
     9781 gcttgaaagc accagtcatt aaaactcatg gagcaacagg ccccgatgct gttcgctata
     9841 cgatccgcca aatccacaca atgcttgaga cagatgtagt tggacaatta gtcgaacaat
     9901 ttgaaaaaag cgaagaatag tatttggata tctgaagcga tccgttccat tgaaaaaaag
     9961 gtggacattc ctgaaaaatg ccagtaaaat tagccatggt gtaggtaatt gacaagtgta
    10021 tgattcttgg aggtgaaaaa gttgactcgc gaagaagtat ttaacaaagt agcgaaaatt
    10081 atttcaaatc attttgagat agataccgat aaagtgacgg atgaattgaa catcaaagat
    10141 gatttaaaag ctgattcaat tagtattatg gaattcgttt tagagcttga agatgaattc
    10201 ggtacggaaa tttcagatga agacgctgaa caaattgaaa cagtcggtgg cgcagtagat
    10261 tatatcagca gccatcttaa ataatttcaa ataaaagcaa agaaggataa aaagctgcat
    10321 gcagcttttt atccttcttt ttttaatctt tttgacacgt aaaagtcatt tttctgataa
    10381 ttctggcgat aaacagaaat tatgttgcaa agtaaagcgg ttttctatat aattattaat
    10441 aattcattac acaaagataa gaataacctt tgttataaaa cgatttattc tgtgtaatgt
    10501 ccttttttat taagaaaaac ttccatttag gaagcagaga ggggtattcg attgtccgat
    10561 aaccaattat tagaagttca gaacctacat acaggattcc gactcaaaga tgactattac
    10621 gatgcagtag atgacgtttc atttacttta gacaaaaacg agattttagc aatcgttggg
    10681 gaatctgggt gcgggaaaag taccttggct actacgatta tgggattact tgatcctaat
    10741 aacacaaaaa ttacaggtga gattttatat aacgatctga atttaatcga cctgaacgaa
    10801 acgctctata ataaaatccg cggaaatgat atcggaatga ttttccaaga tccgttgtct
    10861 gcattaaatc ctctcatgag aatcgaagac cagatcaaag aaggactttc ctatcacaca
    10921 aagatgaatg cagaacagcg tcaagctcgt gcactagaat tgctagaaca agtagggatt
    10981 cctaatcctg cgagagttgg acgccaatat ccccatgaac tatcaggcgg tatgagacaa
    11041 cgtgtcatca tcgcaatcgc aatcgcctgc aaacctccaa tcgtcattgc agatgaaccg
    11101 acgacagccc tagatgtaac catccaagca cagattcttg atttactgaa agaccttcag
    11161 gaagaaacaa aaacagggat catcttgatc actcatgact taggagtcgt agcagaaatg
    11221 gctgatcgag tggcagtgat gtatggcgga cagttcgttg aagtagcagg tgtaaaagaa
    11281 ctgtttgatc atcctaagca tccttataca cgctctttac tgaactctat accacaagaa
    11341 ggaaatcatg atgcagaact ccatgtgatc gaaggtgtcg ttccttctct gaaaaacatg
    11401 cctcgtaaag gttgtcggtt tgcaccacgt attccatgga taccagcaag cgatcatgaa
    11461 gaagagccta tccttcatga agtcgcaccg aaccacttgg tacgttgctc ctgctataag
    11521 catttccact ttagagacga aaaaggagag gtgtagatga cagaattaat cacaatcaaa
    11581 gacctgaaag ttcattaccc tattcgcagt ggattcttca atcgagtaac ggatcatgta
    11641 tatgctgtag atggtgtgga ttttattatc gaaaaaggaa agacatatgg tttagtggga
    11701 gagtctggct ctggaaaatc tactacggga aaagccgttg tgggattaga aaaggtaaca
    11761 gccggacaaa tcatgtatga aggaaaagat gtaacgaagc gaagcaaccg caaaaaaatg
    11821 ggttataaca aggatgtgca gatgatcttc caagattcaa tgtccagttt gaatcctaaa
    11881 aagcgagtgt tggatatcat tgctgaaccc atccgaaatt ttgaacggtt aagcgatcaa
    11941 gaagaaaaga aaaaggtaaa aggactgcta gatatcgttg ggatgccgga agatgcgcta
    12001 tataaatatc ctcatgaatt ttcaggcgga caacgtcaac gtctaggagt agcccgtgct
    12061 gttgctacca atccaaaact gatcgtagcc gatgaacctg tatctgctct ggatctatct
    12121 gttcaagcgc aagtattgaa tttcatgaaa cgaatccagc aagaatacgg cctcagctat
    12181 ttattcattt cacatgattt aggtgtagta aaacacatgt gcgacaatat cgcaattatg
    12241 tacaaaggtc gatttgtaga aatcggtaca agagaagaca tttataatga cccgcgacat
    12301 atctatacga aacgtctgct ttcagctatt ccgcaaatcg atgtagaaaa tagggaaagc
    12361 cacaaagaaa atcgacgaag agtagaacaa gagtatatcc aaaaccaaaa agaatactat
    12421 gatgcgactg gtcgagtcta cgatttgcgt acgatcacaa ctacacacaa agtggcgttg
    12481 aaagatggag gtgcaagcta atgtggaaaa cgattctgcg acgtgtttta ttgatgatcc
    12541 cgcaagttat tattttaagt gttctgatct tcttattagc ccaagcgatg ccgggggatc
    12601 catttaccgg tttgatcaat ccaaatcagg atccggcagt gatcgaacag atgcgacaag
    12661 cagctggctt gaatgaccca tggtatgtcc agtacttccg ttggatcgga aatgctttgc
    12721 aaggcgattt tggtcaaagt ttcttataca aacgctctgt tgctgacttg atcggcgaac
    12781 ggatcatcaa cactttatat ctatcgatct tgacagttgt ccttacttat ttgattgctg
    12841 tgcctctggg gatgcttgct ggccggtatc aaaattcaat cttggataag attgtcgtaa
    12901 tctacaactt ctttagtttt gctgttcctt tatttatctt cggcttgatc atgctatttg
    12961 tttttggtta tcgtttaggc tggttcccaa caagtggatc acaaacacta ggtttgcaag
    13021 gtggatttat cactcaatgg atagaccgtt tgaaatatat tctgttacca tcaattaccc
    13081 aagcattttt ggcgactgcc gtcacaatcc aatatctgcg aagtgaagta atcgattcga
    13141 aatcgctcga ttttgtcaga acagcccgtt ccaaaggggt accgacgaat aaagtattca
    13201 gtcgacatat tttccgaaat gcagcgttgc cgatggcttc tcaaatgggg tatgaaatca
    13261 cttcgttgat tgctggttct gtagttatcg aaaagatttt tggttatccg ggagtcggaa
    13321 aactatttat tgattccatc ggccaacggg actataccgt gatcacatcc ttagtattga
    13381 ttttaggaat cgcgacgcta gtggggacat tactttcaga tatcatcatg agtatcgttg
    13441 acccgcgtat tcggattcaa taaaaccaat cagaaaggag aatggatcga tgagtgaaaa
    13501 aaacaataca gcagttcaag aaagtattcc tcctatgggg atacgaatga ttgctcgaga
    13561 atttaaaaaa gataaaattg ctattttttc attaggtttg ctagtggttt tattattagt
    13621 gatctttatc ggtgcgatgc tgaccgatca agacaaagtg atgtatgtta gtatcttcga
    13681 caaatacgct gcaccaggaa cagtttcgac acaaggacaa aagttcattc tgggagcaga
    13741 tgaaggcgga cgtgatgtat taggacaatt gatcataggt gctagaaact ctgttacgat
    13801 cggatttgcg atcacgatcc ttacttcgat catcggggtt ggattaggga tcatctctgg
    13861 ctactacgga gggatcattg acaacatctt gatgcggatc gttgatttta tcatgattct
    13921 accgatcatg ctgatcatta tcgtcttcgt atcgattatt tccagataca atgtctggtc
    13981 ctttatattc atcatgagtg cattttattg ggtagctaaa gcccgattat tccgaagcaa
    14041 gacattatct gaagcaagaa gagattatgt cagtgcctca aaaacaatgg gaaccaatga
    14101 ttttaaaatc atgttccgcg aaatcatgcc gaatctaagt tcattaatca ttactaattt
    14161 gacgatgaac tttgcggcaa acatcggtat cgagacaact ttgactttct taggtttcgg
    14221 cttgccggca aatgtaccta gtttaggtac attgatcggt tatgcaagta acggagaagt
    14281 cttagtgaat cggcaatgga tctggttacc cgcatcaatc cttattttag ttttgatgtt
    14341 gagtatcaat tatgttggac aagcattcaa gcgttctgca gatgcgcgcc aacggttagg
    14401 ctagtataga agcgaagggg gaaataggaa tgaagaaaac gatttttgga tttgcagcgc
    14461 tgatgtctgc tgtcgttctt gcagcatgcg ggggcggagg tacagatacc tctggaaaca
    14521 actctggagg caacggcaat aacaaagagg aattaacttt tccaattgca acaaaaaata
    14581 atgatgatgc aatcgaagac ggcgaattag aagctgcggt cgcaacggac agtcagttta
    14641 aaggattgtt ccagtgggag ttttaccaag attcatatga tgcagctttt atggctcctt
    14701 ctcatgaacc gctatttgca aacgatgacg atttcagaat cacagacgac ggagctgcaa
    14761 cgcttgatct agatgaagat acgaaaaaag cgacaatcaa agtaaaagac aacgtcaaat
    14821 ggtcagatgg tgaagatgta acggcggacg atgtgatttt cccttacgaa atcattggga
    14881 ataaagatta cactggtatc cgttatgacg acactttccg taacatcgta ggtatggaag
    14941 aatatcatga tggaagtgcc gacacaatct ccggaatcaa aaaaatagac gacaaaacag
    15001 ttgaaatcga atataaagaa gtaaacccaa gcatgcttca atcgggtggt ggtatctggt
    15061 catatgcagc accaaaacat acgttagaag gtgtagcaat caaagatatg gaatcaagcg
    15121 atcaagtacg taaaaatcca gtgacttttg gcccttatta catgagtaat atcgttgcag
    15181 gagaatcagt agaatacttg ccaaacgagc actactggaa tggtacgcct aaattgaaaa
    15241 agatcacgat gaaatctgta ccgatggcaa gtgcaactga agcattgaac tctaaattat
    15301 atgatatgat tttccaaatg ccaacagata cttacgatac ctataaagaa attgctggct
    15361 atacaaactt aggccgtgaa caaacttctt atacttatct aggattcaag ttaggtaaat
    15421 ggaatgcaga tacaaagagt gtggaataca atccagattc aaaaatggct gataaatcat
    15481 tacgtcaagc aatgggctat gcattagata ataatgcagt aggtgaacgt ttctatgcag
    15541 gtttgagaag caatgcgact tcattgattc ctccagtatt ccagtcatac catgatgatg
    15601 agatgaaagg attcacacaa gatctagaca aagcgaaaaa actattagat gatgctggct
    15661 acaaagatgt ggatgacgat ggtttaagag aagacaaaga tggaaataaa ctaacaatca
    15721 actttgcttc gatgtccggt ggggaaacag cgcaaccatt ggcagattac tatatccaac
    15781 aatggaaaga aattggtttg gatgtccaat acacaacagg tcgtctgatc gaattcaatt
    15841 ctttctatga taaattagaa aacgatgatc ctgaaattga tgtttaccaa gctgcttgga
    15901 atacaggaac tgatccaaat ccaagtggac tatggggacc aaatgctgcg ttcaactaca
    15961 ctcgttatga atcagaagaa aatacaaaat tgatcaacga tatcagttca agcaaagcat
    16021 ttgatgaaga gtaccaaaaa gaagcatatc aaaaatggca acaatatgca tttgaagaag
    16081 catttgcgat tccaacgttg ttccgtaatg aagtattgcc agtcaacgac cgtgtcaaag
    16141 actgggattg gtcttatgat gtaccaaacc catgggcaaa tgtttctgtt acagcagatt
    16201 cacgttcata atcactaaaa atgaccgaaa aagggcgatg tcctttttcg gttttttgat
    16261 atttttctgt ttatttctct aatattcagg tcaaggcgct gatttcatga cttctccaat
    16321 aaacggtgtt caaagtccag tttctcggta ttaagtcaaa tttatgcaaa aatatggaaa
    16381 gccattttca caaaattttt tcttcatact cgaaactaaa cgaatttttc acaacttctc
    16441 gtcaatcact aggattatta aagctttact taccttttga ccgaaccatt tcaaatttga
    16501 caaaaaacca gtataataag gaagtatcga aaaaatatcc ataaaaggaa tgtggaaaat
    16561 ggacaaccag ctgacaaaag aactaaaaga aaactataac atcgtttttc acgatctaaa
    16621 tttgcttgaa caagctttta cccattcatc ctatgtgaat gagcatcgca atctgcaatt
    16681 atcggataat gagcgacttg aatttttagg agatgccgtt ttagaactga tggtttctca
    16741 gtatttattc cgtttatatc ctcatttgcc agaaggaaaa ttgacaaaaa cacgagctgc
    16801 gatcgttcgc gaagacagct tgtcgaaatt tgccaaagaa tgtcactttg atcaatatgt
    16861 tttgttgggc aagggagaag agaattcagg aggacgtacg cgaccagcat tgctttgcga
    16921 cctattcgaa gcttttttag gcgcgttata tcttgaccag ggattcgaca cgacccatgc
    16981 attcatcgaa aaagtgattt ttcctaaagt caaagcaggt gctttttcac atgagatgga
    17041 tcataaaaca aaactacaag aagtcttgca aaaatcaggt gatgtatcaa tcgaataccg
    17101 attgatcaat gaagaaggtc cagctcatga acgagtattt tggatcgaag tttatgtaga
    17161 tgatcaattg attggcactg gacaagggaa atcgaaaaaa ttagcagaac aagctgcggc
    17221 ggaaaatgca ttggcagcac tgtaaaagta agtgagggaa agctttgtat ttgaaacgaa
    17281 ttgaaatagc cggttttaaa tcatttgcag acaagacagt cattgatttt gaaaatagcg
    17341 tcacagcagt ggttggacca aatggcagcg gaaaaagcaa tattacagag gctatccgct
    17401 gggtattagg agaacaatct gctaaaagtc tgcgaggcgg aaaaatgccg gatatcatct
    17461 ttgccggttc cgatacccgt aagcagctga atattgcaga agtcacagtg attttagaca
    17521 atagtgatca ttatctgcca ttggagtaca atgagatcag tgtaacccgc cgttatcgac
    17581 ggactgggga aagcgagttt ttcatcaata aacaaagctg cagattaaaa gatatccaag
    17641 aattgttctt agattctgga ttaggaaaag aatcgttctc aatcatttct caaggaaaag
    17701 tagaagcgat tttcagtagt aaaccggaag accgtcgtgg gatctttgaa gaagcagctg
    17761 gtgtcttgaa atacaagcag cggaaaaaga aagcagaaca gaagcttttt gaaacagaag
    17821 acaacctcag ccgcgtccaa gacatcatcc acgaattgga agaacagtta actcctttgg
    17881 ccgcccagag tgaggcagca aaggagtttt tacggctaaa agaaacactg actcaaaccg
    17941 atgtctcttt gatggttgct gaaatcaaga cagcaaaaaa agattgggac aacaaacaag
    18001 cacagcttgc gaaattcaat ctagaattag gcaaactatc tgagtcgatc caagaacaag
    18061 aatccatttt ggcgaagcag cgtaaagaaa atgcgcaagc cgatcgtctg atcgaaaaaa
    18121 atcagcaagt attattagat ctttcggaaa aactgaagca gacagaagga caaaaagatg
    18181 ttttgcagga gcggacaaag catacacaaa aaagctcgca agaatatcaa acttcattag
    18241 cagaagcaca aaaaaaggtt aaacattttg aaaagcttca agaaagcttg atgaaagcag
    18301 cagcagaaaa agaaacagag atccaaaaag ctgaagcaaa tctcataaaa acacagcaag
    18361 aattagaaaa ataccaaaaa tcaacgaaag aattattggc agaattaaga gatcaatatg
    18421 ttgatttgat gcaagaacaa gcagctgtag gaaacgaact gaagtatcta gaacgtcaat
    18481 atatacaaga aacagcaaaa agcaagcaga cgttagccaa gcaatcggaa gtagaagcat
    18541 cagtagaccg attgatctta caaaaagaag aattgacaca aaagcaggcg cagctaaaat
    18601 catctttaac tgaaacaaaa gagaagttag aaatgatcca acaaaacggg aaaaagttcc
    18661 aagaagcatt agcaaaagaa cagcctaaaa tgtatcaatt aatgaatcaa gtccagcagc
    18721 tacgtgctcg taagaaaagc cttcaagaaa tccaagaaaa ctattttggt ttttaccaag
    18781 gggttcgatt ggtcttgcag cataaacagc agctttcggg tattgtcgga gcagtggctg
    18841 aactgatcga tgttcctgca gatttcacgt tagctatcga agcagcttta ggtggggcag
    18901 cccagcatgt catcgttgaa aatgaaaaag atgctcggca agccatcacg tatttgaaac
    18961 agcaaagagg aggaagagca acattccttc cactgacgac aatcaaacca agacaattac
    19021 cggctcatat attgacacaa gcagcagctg tagaagggtt tatagggatt gccagtgaac
    19081 aagtcagtta tcccgatcaa atccaaactg tggttcaaaa cctgctagga acgatccttt
    19141 tagccaaaga cttaactagt gccaatgcaa tcgcgcaaac gatccgctat caatatcgag
    19201 tggtttcctt agaaggtgat gtgatgaatg ccggaggatc catgacaggt ggagcgaata
    19261 aacgaggaaa ccaaggcagt ttgtttgtac aaaatcaaga attgaaacag ttgactagtg
    19321 aatttgaaga agcagataaa caactccaag cacaagaaaa aaaagttcag gagctgcaac
    19381 aagaaacagc tcgtttagcc gaagaacaag aagttttgcg tacacgaggc gaacaattac
    19441 gttttgaaga acaagaagcg acaaatcagc tacaaaacat cataaatgaa cttgaacgtt
    19501 ttgaaaaaga aaagcaaatc tcaacttttg agacacgtga actgcagcag tttatcgaag
    19561 attacgaaaa gcaaacgaac gaattgaaag acaaacaaac agatcttgaa tcgcaacgac
    19621 aaaaaatcga cgaagaaatc aaatcgttaa gtcaagaaag cgaccagatg gaagccagaa
    19681 gagcacaagt acaaagtcaa aaagcacaag aacaagcaga tcttgctgtc ttgaaagagc
    19741 agtttaatca tctgcagatc cagctccgcg gtgcccgagt ccaaaaagca gaggcaaccg
    19801 aacgtcaaga agcgatagaa aagcagttgg cgacattgac agcagacttt tctgatcatg
    19861 aagtcacaga agaaagtttg gaaaaacaaa ttaacgaatt atctgctcag cgagaaacat
    19921 taaaagccga gttggcaaaa gccaaagagc agcgagatag aaggcaaaag gagatcgata
    19981 agcttgaagc agtcttggca gagcgtaatc gtgagcaaaa agcacgactg tcggaacaat
    20041 ccaaattaga agtccaaaaa gaccgagctg aaatgatttt agataatcat ttgacctatc
    20101 tgcagtcaga atatcagctg acatttgaga aagcttccca agactatcaa gaaacaaccg
    20161 atatcgaaga cagtagaaca aaggtttcat ccttaaaaga acagattgaa aaactgggac
    20221 cagtaaactt aaattcaatc gaacaatacg aacaagtcag tgaaagacat acattcttag
    20281 caacacagcg agatgatctg ttagcagcga aaaaccaatt gtttgaaacg atggatgaga
    20341 tggatgatga agtgcggaca agattcaaag aagtatttga agctatccgt caagaattca
    20401 aaattgtctt tccaaatatg tttggcggcg gtaaagctga attagtactg acggaaccaa
    20461 ctgatttgct gaaaacaggt attgagattg aagtacaacc tcctggcaaa aaactgcaaa
    20521 gtctaagttt gctttctgga ggagaacgag cacttacagc catcgcgttg ctcttttcca
    20581 ttatccgtgt ctgtcctgtt ccgttttgta tcttagatga ggtagaagca gcgttagatg
    20641 aagccaacgt gaaacgtttt ggtcgttact tgagcgattt ccaagatgac acacaattta
    20701 tcgtagtcac acaccgcaaa ggaacgatgg tcgcagcaga tgtactgtac ggagtgacga
    20761 tgcaggaatc cggtgtatca aaaatcgttt ctgtacggat ggaagatatc aatgaagagg
    20821 cgaaaatcaa cgaaacgaaa gtgtaggaac gaaaatgatc aaattagtag caatcgattt
    20881 ggacggtaca ttattaaatg aacaaaagct gatttcagat gagaataaac aagcattagc
    20941 acaagcaaaa cagcaagggg tcaagatcgt tctatgtaca ggacgcccct tagctgcgat
    21001 ggcgcattat ctgcaagaat taggactggt agatgaaggt gactacagca tcactttcaa
    21061 cggcggtttg gtacaaaaaa atgatactgg tgaaatcatg gaaaagaaag tgatggaagt
    21121 atcagatatc caacgcttat acaaactggc tcagcaattg gatctgccac tagatgtttt
    21181 gtctgacaat gtggttttac agctgcctag tgccccaagg aagcaatcct tgtacaatgt
    21241 gttgaataag ctgctgcaat tccaaccagc ggatataagc gatattacag atgaatttgt
    21301 gcttaataag gcagtaatcg cttatgacca agaagaatta gatcctaaaa tcaaagaaat
    21361 accagcagaa ttccatgaaa tgtatgagat catcaaaaca agaagtatgc ttttggaatt
    21421 tatgccaaaa ggtgttacaa aagcttatgg tatttcttta ttggcaaaag atcttggttt
    21481 gaaacaagaa gaaatcatgg cgatcggtga tgaggaaaat gatttaccga tgatccaata
    21541 tgctggacta ggcgtagcga tggagaatgc tgtgcctttc gtcaaagaag cagcagattt
    21601 cgtgacagcc agcaatgaag aaaatggcgt agccaaagtg atccaaaaat ttgtattaga
    21661 gtagggggag tcaaatggga ttttttgata aaatcaagcg tgcttttact ggcgaagaga
    21721 aaacagaaga agttgaagta caggagaagt acgacaaggg tttagaaaag acgcgtaaaa
    21781 catttggtga acggatgaat gagttgtttg ccaattttcg aacagtcgat gaagactttt
    21841 ttgaagaatt agaagaaaca cttatcggtg cagatgtagg ttttgaaaca gcgatacgaa
    21901 tcaccgaagc attacgtcaa gaagtgaaac tcagaaatgc gaaaaaatcg gcagaagtcc
    21961 aaaatgcgat catcgagaaa cttgtggatc tctatgaagc ggaaggaatc aatgaagtaa
    22021 atgaactaaa tatccaagaa gacggactaa cagttatttt gtttgtcggt gttaatggtg
    22081 tagggaaaac aacaagtatt ggtaaacttg cccatcagtt caaaaacgaa ggtaaaaaag
    22141 tattgatggc agcagcagat accttccgcg ctggagcaat cgaccagtta gttgtctggg
    22201 gcgaacgcgc tggtgttgaa gttgttcgtg gcaatgcagg aggcgatcca gcagctgtcg
    22261 tattcgatgc tttggaaaga gcaaagacag agcaggcaga tgttttacta gttgatactg
    22321 caggccgact gcaaaataaa gtgaacttga tgaacgaatt agaaaaaata aaacgagtaa
    22381 tccagcggga attaccagaa gcaccccaag aaacacttct tgtattagat gcaaccactg
    22441 gtcaaaacgc gatggttcaa gcaaaacaat tcaaagaaac aacagatgtg accggtcttg
    22501 ttttgacaaa attagatggt acagcaaagg gcggtatcgt acttgctatc cgtaatgaat
    22561 tgcatttacc agtgaaattg gtcggtcttg gtgaaggaat cgatgacttg gagccgtttg
    22621 atccaaatga ttttgtcgtt ggattgttca aaggattact aaaagaagaa taacccatgt
    22681 ctaaaaagca ggagctgtga caaaagttgc agctccttgt ttgaatgatt gtttttaaca
    22741 tagctgtgtt tttgatgcct tcatttttat acttatttgt aagcggttct caaacaattt
    22801 cgttcttttt cttagacttt ttgattataa ttagattcat atgaattgga ggtcacttaa
    22861 tgatgaattt taagcaatta ttattgcatg tcaatgcgcg cccttttatc gaccaagctc
    22921 gatttggtat agaacgggaa ggacagcggg ttgatcttgc aggaaatcta gcaaaaaccg
    22981 atcatccagc aatctttggc gatcgatcct atcaccccta tatccaaaca gattttagcg
    23041 aaacacaaac agagatgatc acccctgtta ccgattctat tcccgaatta tttcagtatc
    23101 tcgctgctgt ttatgatgtg actgctcgtt ctataccgaa agaagagatg atctggccat
    23161 taagtatgcc acctgcctta ccggaaaaag acgaagagat cattattgca aaattaaaaa
    23221 atttcgaaga tgtcttgtat cgacgttatt tagcaaaaga atacgggaag cgaaaacaga
    23281 tggtaagcgg tattcatttc aattttgaat ttggcgatga gttgctaaga acattgttca
    23341 gccatcagga agaatttcaa gatttttccg aattcaaaac agaactttat ttgaaaacag
    23401 cgagaaactt tatgcgctat cgctggatga tcacttattt attcggtgct tcaccaatga
    23461 gtgagaagaa ctacttttta gatgaatcac atccgcaaga acctgttcgc agtattcgca
    23521 acagtgcgct gggctatacg aatcatccaa atgtgaaagt ttcctatgct tctatgaaac
    23581 agtatttagc agatatcgag cgaatgatcg aagaagggaa actttcggag gagaaagaat
    23641 tttatacacc gcttcgtttc cgaggcggaa agaaagtcgc agatttagca acaacaggtg
    23701 ttcgttatat tgaattgcgg aatatcgatt tgaatcctta tgcaagattg ggtatcaatc
    23761 cagagcaagt tcggttctta caattattcc tgatgtacat gttatggaca gaagaaaaag
    23821 aagactgtga ccaatgggta gcagaaggta cgactcgaaa taacaaagta gcactagaac
    23881 aaccatcaga tcaaacagaa tttcatcaag aaggtagaga aattcttgaa ggaatgaagc
    23941 aaatgctggt tgaattggat tggttggatt ctctttattt agtagaggaa gcattgactc
    24001 aaatggatca tcctgaacaa acgttagcag cgaaactcta tcaagaagca cagctatcga
    24061 gtcagcaaga agttgctgtg gcattgggac atcaatatta taaagaaagc catgaacgtc
    24121 cctatcagtt ggccggtttt cgcgaaatgg aactttctac tcagatattt atgttcgatg
    24181 ccatccaaaa aggtgtccaa gtcaaagtat tagatgagtc ggatcaattt ttgcgtctgc
    24241 aattccaaga ccatgtagaa tatgtgaaaa atgcaaacat gacaagtaag gacagctata
    24301 tcgtcccact tatcatggaa aacaaaacag taacgaaaaa agtcttaaaa gaagctgggt
    24361 tccgggtacc aggaggcgca gaattttcat ccatggaaga agcggtaaaa gcttatccga
    24421 gatttgccga gcaggcattt gtgatcaaac ctaaatcaac caattacggg ttaggtatca
    24481 cgattttcaa ggaaggagct agtctggaag attatcaagc aggtttagcc attgcttttc
    24541 gtgaagacag ttcggtttta gtagaagaat ttatgccggg aacggaatat cgtttctttg
    24601 tgatcgacgg agaagtccaa gccatcatgt tgcgagtccc tgctaatgtt attggtgaca
    24661 gtatccgaac agtgaaagaa cttgttgaag aaaaaaatag tgaccctttg cgtggaacga
    24721 atcatcgtgc acctttagaa ttgatccaat taggtgaatt agaacaattg atgttgaaag
    24781 aacaagggtt gacgatcgaa tcagttcccc aagccaatca aattgtttat ctaagggaga
    24841 actcaaatat cagtacaggt ggtgattcga tcgatatgac tgatgaattt tctgaagctt
    24901 acaaaaaaat cgccgtttct gcggtagaag cactaggagc taagattagt ggtatcgatt
    24961 tgattatacc agataaagaa atcgatccta caactgataa aaaagcatat gggatcatcg
    25021 aagcaaattt caatccagcc atgcatatgc atgtttatcc ttttgcagga aaaggcagaa
    25081 gattgacgat gaatgtattg aaattgttat atcctgaagt attttaaaag aaaaagcttt
    25141 tgacagattg tcctatttga tgtcaatctg atcaaaagct ttttttgcat gtaaaaattt
    25201 atgtgttttt tcgttttaaa ataaacgatc ccagaagctt tttttctttt ttggtttttc
    25261 tttttcagct ggcgaagagt tttcttctgg gtattttttt tcgatatcaa tcacaggttc
    25321 atttactgct tcttttgctg ttaagagcaa tccgaattcc tcaggatttg cagtgacgta
    25381 atcgttgact acagtgaaac ggctattgtt tttcgttgct agctggatat attggttttg
    25441 gacagaaagg ggtagagccc cattcaagag tatggaagca tctggatgtt ttttgagttc
    25501 ttttaaaaag ttttctttgt tttttggttg gatcatttca gcaacagtca tactaagata
    25561 gcagcgttcc ctgaatgtcc ctaaatattt atgctgttct tcagggttca ctaaaggtgt
    25621 accataaagt cccttatcca aatggctttt tacatcatct tgtgccataa aaaataccct
    25681 ccttcttctc tttgtctttc ttacgattat agcatattcc caataatacc taaaacatat
    25741 gaaaaagcgg ttgcttttgt tcaacgattc acttaaaata agaatgaaaa tcattctcaa
    25801 ttagaaaggt ggattggatg cgctacggac gactagtagc attattagtt attttaagta
    25861 gtatttcatt gttcatcgga gtatcgaatg tacaattgtc agaactgctc cagttagatg
    25921 ggcaaagctt acaaatcttg ctagtgactc gtttgccacg aacgatttgt ttgatccttg
    25981 tgggagcgac cagtagtatt tgtgggttga tcatgcaaca tttgacccaa aataaatttg
    26041 tttcaccaac gactgcgggc acgatggata gtgcgcgttt aggtatttta gtggctatga
    26101 ttttcctacc tggtgcatcc ttgattgtcc gatccttgac agcattttgc tttgcttttg
    26161 caggaacatt gcttttttta tcccttacgc gattgttcct ccagaaaaat caattgatgc
    26221 ttccgttgat tggagtcatg ttagggaata tcattggttc agttgctact ttttttgctt
    26281 atcaattcca gttggtacaa aatatgactt catggcttca agggaatttt gcgactgtga
    26341 tacgaggaaa ttatgagtta ttgtatgctg ttgttccatt gttgctcttg gtttggctat
    26401 ttgcttaccg atttacgatc attggtctgg gcgaatcgtt tgcagtaaat ctgggaatag
    26461 attaccagaa gatgcagttt ttaggcattg gcttagtatc attggctagt gcagtcatgt
    26521 tgatcatggt aggaagcatt ccttttatcg gagtgatcat tccgaatctt gtctctagaa
    26581 tatatggaga tcaagtgcac aaaacaattg gcgtaactgc tttagcaggt agtttgttct
    26641 tagtattttg cgatcttgtc gctcgcgtgt tgatttttcc ttatgaaata ccagtcagtg
    26701 tgatcgttgg aatcatcggt ggggctatct tcctttatct tctgataagg ggacgacgat
    26761 atgcttaaaa aaaagccatc tatttttatt ggtatgcttg ttcttgtttt atttgttaca
    26821 ggaatgtact tgacgatcca tacatatgga aactggcatt ttgcatgtgc attgaggagt
    26881 aagaaaattt ttgcatttat ccttgtctcg ctagctgtca gcagtgccac aatcagcttt
    26941 cagacaatta cgcataacca gtttttgact ccttcagtat tgggacttga gtcgttatat
    27001 gtccttgtcc aaacggtcct tttcttcttt atcggaggag tgacgatgct tcagcagaca
    27061 ggtacagttt ttttcttatt gaacgttggg ctgatggtcg ggctgagtct attgttattt
    27121 ttctttcttt tgaaaaaaat gagagcgaat ctttttttat tactgatgtc agggatgatt
    27181 ttaggtacgc tttttggtag tgtcagtacc tttttacaag tcatcatgga tccgaatgaa
    27241 tatgatttgc ttcaaggcag attatttgca agctttggca atatccagag tcagtatctt
    27301 ttgcctgctt ctttgatcat tatcggctgc tggtacctgt tatttcgata tcgacatgtg
    27361 ctagacgttt tccatctcgg aaatgaacaa gcagtaaacc tcgggataga cacagatcgt
    27421 tttgaatttt tgattttatg ttgtgtcagt cttttgacag ggacatcaac ggctcttgtg
    27481 ggtccagtta cttttttagg gtttattgtc gcaaatatca gttatcgtgt cttttctact
    27541 tatcgtcatg gtcagctatt tatcggaagc tttctggtag ctatgctgtt tctgacagga
    27601 ggacagctcg cagtagagca gattttcggc tggaatacaa cagtgagtgt cgtcgttgaa
    27661 tttgtcggag gaatttattt tgtcacaaaa ttgattcgcg aaaggaagag tatcctatga
    27721 ttgatttaca aaaggtcaat aaactgtacc atggaaaatc agtggttaaa gatgtacagt
    27781 taacgattcc tgaaaagaag ttgactgctt ttatcggtcc aaatggtgct ggaaagtcaa
    27841 cggtgttgtc gatgatcagt cgtttgatac caaaagatac aggtgaaatc tatttagacc
    27901 ataacgaagt aaaggcttgg aaatccaatg aattagccaa aaaattatcg atcctacgac
    27961 aatcgaatga aataaatgtc caaatcaccg tgaaagaatt agtagaattt ggacgatttc
    28021 cttatacgaa aggaaaattg acgacaaaag acgaggaaat cgttcaggaa tcactggaac
    28081 acattgggtt agaagaaata gcggatcaat cgattgacac tctttcaggc ggtcaactcc
    28141 aacgtgcgta tattgcaatg gtgctagcgc aagacacgga ttatatttta ctggatgaac
    28201 ctttgaacaa tctagatatg aattttgcgg ttcaaatcat gcagatttta aaaaatttag
    28261 tacaagaaat cgggaaaaca attgttattg ttcttcatga tatcaacttt gctgcaagtt
    28321 atgcagacga aattgttgca atgaaagatg gacagttatt tacgcaagga acgacccaag
    28381 aaatgataaa aaaagatgta ttggatgaat tatacgaaat ggacatacgg atttgtgaga
    28441 ttgaaggccg cagattctgt ctttatttct aaaactaaca ttaggagaga agagatgaaa
    28501 aaattagtag taggtatctt gattctagca tcatttggtt tagcagcatg tggaaatagc
    28561 aacaacacca gtcaggcaga cacaaaaaaa agcagcacac aaactgaaac tacattgacg
    28621 ataacagata gtaatggaga ccaaatcgag gtgccaaata atccagaaaa agtcgtcgta
    28681 ttcgacaatg gttctttaga tacgatggat gctcttggtg ttggagataa ggtagttgga
    28741 gcagctacaa gcagccttcc tgagtatctt tcatcctata aaaaagtaga atcagcaggt
    28801 ggaataaaag aaccagactt agaaaaaatt aatcagcttc agccagatct aatcatcatt
    28861 tctggccgac aaagagattt tcaaaaagat ctaagtcaaa ttgctccgac cattttcctt
    28921 tctttagatg cgaaaaatcc atgggaatct ttccagcaaa atgtcactgc tttaggggag
    28981 atctttggta agcaagaaga agctaaaact caattagagg aattatctag tgcaatcgat
    29041 cagacgaaaa agaaagcaga agctactgac aaaaaagcac tagtcacact tgtgaatgaa
    29101 gggcaattat cagcctacgg atctggttct agattcggtt tcatccatga tttatttggc
    29161 tttgaacaag cagatgatca aatcgaagca tccactcacg gtcaaagtgt ttcttatgaa
    29221 tatgtgctgg aaaaaaatcc ggatatctta tttgtagtag acagaacaaa agctattggt
    29281 ggagacgaca gcaaagatga tatttctgcc aatgaactgg tagcacaaac gaatgctggt
    29341 aaaaatcagc agatcatctc ccttgaacca gatgtctggt atttaagcgg gggcggttta
    29401 gaatcaatga aactgatgat cgaagatgtg aatcaagcat ttaaatagca taaataacag
    29461 aggattaaac tctcatacta tgtaacaata atgtttctta cattcttaaa tagcaagagc
    29521 ctatcctcaa agaagttatc aaaaaccatc cgtaaaatcg tattgatgat tttacggatg
    29581 gtttttattt atttgagaaa tcttttttca acgtgaattc attatctaca tcatacgggt
    29641 tgattctaga atggggagtg gcaaaaaata gtgtgatcca ttttttcagc atcaagatcg
    29701 cctcctttga tttgaatcaa aggtacaaag cgaaacttag ataaaactta aatttagata
    29761 tttttatttt gtaccgatct cttctttaaa cgatgttcca tctgctgctt ctcggcaata
    29821 agtccactag actagaaaat atgagaagct attttctgtc cagcgttctt attactcaaa
    29881 acaaatcgca gttgtcacaa tctcttctaa acgatgttcc atctgctgct tctcggcaat
    29941 aagcccacta gactagaaaa tatgagaagc tattttctgt ccagcgttct tattactcaa
    30001 agcaaatcgc agttgtcaca atctcttcta aacaatgttc aatcagctga ccttcggtat
    30061 tagctcaaag cactgatttc acaacctcta tttaggcatg tgtctcagca agcggttgat
    30121 agttgatgat ctgcattttt cgataaaaat cggcgggaag cttactccac cgttttagta
    30181 attgatagaa ataactctct tctgctttga ttttatactc gcttaatatc aatccaaaac
    30241 tattcgtttt ttttgaagct aattttttat aaatatccaa tgctttggtt ttatcttcat
    30301 tgacaaagag tgtttttact tcaatataag aaccctcgat attttgtgtt tcttcgataa
    30361 aatagatgtt ttttatatat tccttcatag tgattcctcc aaagaagtct tttatttgat
    30421 aagtttactc taccgaagag ttgttaataa tccgtgaaca aaaagtaatt ctttttccaa
    30481 tagttgttaa aggtttttta ttttttatag tattttttgt tatttaagaa aggaataagg
    30541 ttaaacaaat aacaggttgc tttttgggtc tttttccgtt caagaaactg aacgaagaaa
    30601 agggaaagtt gtctcttttg tgataagaga aatcataata aacgaatgac ttactttcca
    30661 ataaattgag aacagtcaga accagacaaa ttagttgcat ctgatgaata aagtgattat
    30721 tctatgacat gaggtgatac agatgaaatt agaagtgaca aaagaagcac aagaagtatt
    30781 gaagaataaa ttagaagcag acgatcaact tttattagat atcgaaaacg gtgacggtcc
    30841 atttgcagaa agccaagtca gttgccaatt ggatacggca tttcgattga tcattgtcaa
    30901 aaaagataca caaacagatc taagtcttta ttctgtagtg gctgatacac cagttggacc
    30961 aatcaaaatc aaagacagtg caatacttta catggatgat ccatccacac ttgctttaga
    31021 accaacttat aatagtttac aattaaaagg accaagcggc ttgagaaaag gcaacttgca
    31081 agttgttcga aaagactaga gggataaaat gggaaaattg gcgatcatca gcgacctaca
    31141 tgtggacatc aataaattcg gcgaatcaga gctgatgcag ctttggcaag ttttgcaaaa
    31201 gcacaagatc actcggcttc atctagccgg agatacggct aataaagtag accgttgcct
    31261 agctgttgtc gaatttttcg tcaataaagg attatctacg acatttcatt tcggcaatca
    31321 cgagttggca gatgtgccca atgaacagat gatggaccat tttccagacc cacatttttt
    31381 aaatgaacgg tatattgctt taaatgagca gaccgttctt ttagggatga atggctggta
    31441 cgactatcag ttttccgaat tgaaagatta cgaggcaatc gtacgaatga agcgtttgta
    31501 ttggtatgat cggttcatcc agcgtgggaa aacagatctc gaggtcaatg cagaaatgct
    31561 tgcagctgca aaaaatttgc tagaccagct ggaagcaaaa aaacttgagg tgattttatc
    31621 cactcatttc gttcctaaac gggcatttat catgtatcaa tctgctcctt atgagcgttg
    31681 gaacaaatta aatgcatttt taggatcaga atcattcgga gaattacttg accgttattc
    31741 aaatatcaaa caagtagttt ttggccatac gcatcgtcgc tttgaagata aatccatcgg
    31801 tcacacgatt tacagctgca gaccttttgg ttattattat gaatggcagc tgacccgaga
    31861 ttttgtcttg aaagaacagt tgctagacag ctatgatcct atgaaattac gtgtcctctt
    31921 gcgtgcccat cacccagctt ttcaggccta tcaaacacaa catttgcaag aggaatttga
    31981 acaagcgatg acaatcgttc cgtattgaga cagcattagt aatgtgtaaa agaaaatacc
    32041 ttgtaaaaca taaggataag taagagcagt agatccattt atcgtggata tgctgctttt
    32101 tttgcggaaa ataatcagcg aatgtttatt tgatcggatg atttgtaact cgcagagtac
    32161 tagatgcaag cgtgtattct ttttttgaac gataatcaca aaaaaaaata taatatacat
    32221 tttttattta attataatta atattgattg aagtctaggc agtcaagtcg agagtgtaaa
    32281 atattttgtg taaatgaaaa aatccataca aaaaaggaag tc
//
LOCUS       DAZKOU010000024        32246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000024 DAZKOU010000000
VERSION     DAZKOU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.024"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG827_001059"
     CDS             complement(99..1229)
                     /locus_tag="ACG827_001059"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0483217.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG827_001060"
     CDS             complement(1878..3080)
                     /locus_tag="ACG827_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0483218.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG827_001061"
     CDS             complement(3140..3343)
                     /locus_tag="ACG827_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0483219.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4507)
                     /locus_tag="ACG827_001062"
     CDS             complement(3485..4507)
                     /locus_tag="ACG827_001062"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0483220.1"
                     /translation="MIDYLTIHFFSNQHLKLIEEIIEMPIERFEELDYAPKGYIGQYV
                     WNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQFFRKVLDYQGNFTRI
                     DFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNESNGETLYLGSKKSQ
                     CRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKIISKTHDLTKLFFEL
                     LNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSFEKTMSWLIHGVSPS
                     LVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQEEVEYYERKLQSIS
                     NEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG827_001063"
     CDS             complement(4610..4717)
                     /locus_tag="ACG827_001063"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0483221.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG827_001064"
     CDS             4880..5692
                     /locus_tag="ACG827_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0483222.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG827_001065"
     CDS             complement(6066..6257)
                     /locus_tag="ACG827_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483223.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(6502..6732)
                     /locus_tag="ACG827_001066"
     CDS             complement(6502..6732)
                     /locus_tag="ACG827_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483224.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            complement(6716..6913)
                     /locus_tag="ACG827_001067"
     CDS             complement(6716..6913)
                     /locus_tag="ACG827_001067"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0483225.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="ACG827_001068"
     CDS             complement(7059..7937)
                     /locus_tag="ACG827_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0483226.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="ACG827_001069"
     CDS             complement(7976..9094)
                     /locus_tag="ACG827_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0483227.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="ACG827_001070"
     CDS             complement(9091..11190)
                     /locus_tag="ACG827_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0483228.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="ACG827_001071"
     CDS             complement(11241..12062)
                     /locus_tag="ACG827_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0483229.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="ACG827_001072"
     CDS             complement(12059..12868)
                     /locus_tag="ACG827_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0483230.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="ACG827_001073"
     CDS             complement(12889..13383)
                     /locus_tag="ACG827_001073"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0483231.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13895)
                     /locus_tag="ACG827_001074"
     CDS             complement(13395..13895)
                     /locus_tag="ACG827_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0483232.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(13960..16656)
                     /locus_tag="ACG827_001075"
     CDS             complement(13960..16656)
                     /locus_tag="ACG827_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0483233.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="ACG827_001076"
     CDS             complement(17084..18157)
                     /locus_tag="ACG827_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0483234.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="ACG827_001077"
     CDS             complement(18154..18981)
                     /locus_tag="ACG827_001077"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0483235.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="ACG827_001078"
     CDS             complement(18982..19788)
                     /locus_tag="ACG827_001078"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0483236.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="ACG827_001079"
     CDS             complement(19800..20885)
                     /locus_tag="ACG827_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0483237.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="ACG827_001080"
     CDS             complement(20902..21444)
                     /locus_tag="ACG827_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0483238.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="ACG827_001081"
     CDS             21702..22691
                     /locus_tag="ACG827_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0483239.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="ACG827_001082"
     CDS             22688..23425
                     /locus_tag="ACG827_001082"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0483240.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="ACG827_001083"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="ACG827_001083"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0483241.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="ACG827_001084"
     CDS             23989..24621
                     /locus_tag="ACG827_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0483242.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="ACG827_001085"
     CDS             24706..25353
                     /locus_tag="ACG827_001085"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0483243.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="ACG827_001086"
     CDS             25358..26341
                     /locus_tag="ACG827_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0483244.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="ACG827_001087"
     CDS             complement(26744..28258)
                     /locus_tag="ACG827_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0483245.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="ACG827_001088"
     CDS             28396..29202
                     /locus_tag="ACG827_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483246.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="ACG827_001089"
     CDS             complement(29377..29946)
                     /locus_tag="ACG827_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0483247.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32089)
                     /locus_tag="ACG827_001090"
     CDS             complement(30029..32089)
                     /locus_tag="ACG827_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0483248.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
BASE COUNT         9659 a         6753 c         5489 g        10345 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttaccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgagccat ttgttgtttt ttttctgaca
    30481 agctttcttc ttgtcttttt tgttcttttt gatctatctg tttgtttaat ggtaaaacat
    30541 gtaatagtga ctgagcatgg agttcttgga gtgaatacgg gtcatgataa ctatacataa
    30601 tcgtaaaatc acctgtttgt ttggtcaaat aaccatcctc caatggtact tcattgattg
    30661 taaccgatac atcctttaac ggtacgggtt tacctaagga atttgttgct gattggatca
    30721 actcttcttt gtttattttt tcgtttggat gaacaataag atcatgacct tctaatgaac
    30781 gattgtctgg tatataaaca taagtaaagg taatttcccc tcctgattgc ttcctattat
    30841 tcatcagttc tgagtgaagt acttcaagga tctccgacca gtgacttttt ctgtctaatc
    30901 cgtttgcttg catccaatca gaaatagaag tgactttccc attttgttca taagtcgata
    30961 aatctatatt tggcactcga tcagaaaatt cagcaggtag gcgcaaatca ggaatacgat
    31021 ttacactact catatgtgtt tctgcttgaa gttctttatt tgtttgacta tctataaaat
    31081 gagtgatgac agtcgatgaa gcagcagatg cttctacata aactacagct gttgtttctg
    31141 ccacatttga tgttttcgag tctatataag ttaatacaac gggatattct cccggagttg
    31201 tagtatctac tgtcccgttc attttgattt cttctttatt tcctgaagtt ccatcgcttt
    31261 ttgttacata ttcaacaaga tctttagggg cgtaactctc tcctgttctt aagtgaagat
    31321 cttttccttt gatagctgtt tgatcagcac gaacatggat cacagcagat gcagtcattg
    31381 tttttaaagc aattggatca aaataggaca aggaaatagt agatgttcct gtattagtcg
    31441 tatccacgat attcccattg tttgaaatta ctttggacat atctccttgt gttccatctg
    31501 cgttcttcag cctttctacc agatcagata tcgaaacttt tgtccctgcc agaacatcga
    31561 ttttctttct ttcaaactgt gaataatctt tttcatatac ataagaaact tgtgcgcttc
    31621 caccgtcctt tatatctgtc aaagcatgat tcaaaatatc agtgatctgt tttaactgat
    31681 caccttttgt aggatcagtc atatcggaaa agatagaaag cggcacactg cttccggttt
    31741 ccgatgtcca taatgttttg ttaggaatta accagtagtg gtttatcgtt ggaggataac
    31801 tgactttatt ataggaagtc aatgcaggaa gtagctgttt tccatttgca tcagtgaaag
    31861 tatgagtaat agtgataggg gaatcagtgg tctcagaaac agaggtacgt acgaccttga
    31921 tggtttgtgt cgttgcctga acgggtacat gcatatagac acctacagat aaactgatca
    31981 gcatcgtgcg catcataaac ttaacgtagt gtttttcaga acacgaccgt tccttatcta
    32041 aacgacaagc gtgtttattt agttgttttg aacgtctttt tgaacacatt tattcttagt
    32101 cctccctact cttgtctaaa catgaagtag taattttcat aagatgattt cattatataa
    32161 ataaaatcaa aagaagatgg actattagga gagtattttt tttgattttt tattgtttca
    32221 agaaaatcgg aataataact tatgta
//
LOCUS       DAZKOU010000025        32002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000025 DAZKOU010000000
VERSION     DAZKOU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.025"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            364..684
                     /locus_tag="ACG827_001091"
     CDS             364..684
                     /locus_tag="ACG827_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0483249.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            838..1743
                     /locus_tag="ACG827_001092"
     CDS             838..1743
                     /locus_tag="ACG827_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0483250.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1740..2513
                     /locus_tag="ACG827_001093"
     CDS             1740..2513
                     /locus_tag="ACG827_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0483251.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2694..4118)
                     /locus_tag="ACG827_001094"
     CDS             complement(2694..4118)
                     /locus_tag="ACG827_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0483252.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4329..4775
                     /gene="nrdI"
                     /locus_tag="ACG827_001095"
     CDS             4329..4775
                     /gene="nrdI"
                     /locus_tag="ACG827_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0483253.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4840..6822)
                     /locus_tag="ACG827_001096"
     CDS             complement(4840..6822)
                     /locus_tag="ACG827_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0483254.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6836..7732)
                     /gene="murQ"
                     /locus_tag="ACG827_001097"
     CDS             complement(6836..7732)
                     /gene="murQ"
                     /locus_tag="ACG827_001097"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0483255.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7735..8796)
                     /locus_tag="ACG827_001098"
     CDS             complement(7735..8796)
                     /locus_tag="ACG827_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0483256.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(9007..9534)
                     /locus_tag="ACG827_001099"
     CDS             complement(9007..9534)
                     /locus_tag="ACG827_001099"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0483257.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9601..11103)
                     /gene="eat(A)"
                     /locus_tag="ACG827_001100"
     CDS             complement(9601..11103)
                     /gene="eat(A)"
                     /locus_tag="ACG827_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0483258.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11361..12482)
                     /locus_tag="ACG827_001101"
     CDS             complement(11361..12482)
                     /locus_tag="ACG827_001101"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0483259.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12608..13549
                     /locus_tag="ACG827_001102"
     CDS             12608..13549
                     /locus_tag="ACG827_001102"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0483260.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13699..14595)
                     /locus_tag="ACG827_001103"
     CDS             complement(13699..14595)
                     /locus_tag="ACG827_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0483261.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14864..16204
                     /locus_tag="ACG827_001104"
     CDS             14864..16204
                     /locus_tag="ACG827_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0483262.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16422..16865
                     /locus_tag="ACG827_001105"
     CDS             16422..16865
                     /locus_tag="ACG827_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0483263.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17369..18787)
                     /gene="nhaC"
                     /locus_tag="ACG827_001106"
     CDS             complement(17369..18787)
                     /gene="nhaC"
                     /locus_tag="ACG827_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0483264.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19190..20413)
                     /locus_tag="ACG827_001107"
     CDS             complement(19190..20413)
                     /locus_tag="ACG827_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0483265.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20444..21283)
                     /locus_tag="ACG827_001108"
     CDS             complement(20444..21283)
                     /locus_tag="ACG827_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0483266.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21300..21980)
                     /locus_tag="ACG827_001109"
     CDS             complement(21300..21980)
                     /locus_tag="ACG827_001109"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0483267.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21982..23055)
                     /locus_tag="ACG827_001110"
     CDS             complement(21982..23055)
                     /locus_tag="ACG827_001110"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0483268.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23111..23349)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23392..23625)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23702..24373)
                     /locus_tag="ACG827_001111"
     CDS             complement(23702..24373)
                     /locus_tag="ACG827_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0483269.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24460..27108)
                     /gene="mgtA"
                     /locus_tag="ACG827_001112"
     CDS             complement(24460..27108)
                     /gene="mgtA"
                     /locus_tag="ACG827_001112"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0483270.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27450..27624)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27797..28234
                     /gene="ndk"
                     /locus_tag="ACG827_001113"
     CDS             27797..28234
                     /gene="ndk"
                     /locus_tag="ACG827_001113"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0483271.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28256..29122)
                     /locus_tag="ACG827_001114"
     CDS             complement(28256..29122)
                     /locus_tag="ACG827_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0483272.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29282..31375)
                     /locus_tag="ACG827_001115"
     CDS             complement(29282..31375)
                     /locus_tag="ACG827_001115"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0483273.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         9280 a         6681 c         5666 g        10375 t
ORIGIN      
        1 ttttttatca caaaaattat cttacttatt gtaaggtaaa gcttttttta atttattgcc
       61 cgataatata tagaaataag gtctagttac tgtttttttt aaatgattaa taaataacaa
      121 cttctttcta tgaaattgca aagcattcat cttcttttcc gctaacgata tatccgtcag
      181 tgcttggtac actttactaa aaagtatgag attgccgata tctggatagg tgtgctatac
      241 tacagaaaaa gatatacaat ctgccaccgg gtaggataaa gaggatgatc attccattag
      301 gtctttgaag ggatgatttt cctctttttg tgtatttttt tatagttaag gaggaatgta
      361 agtatggcat ggatggaatt aatcatagca ggaattttgg aagtattctg gtcaacgatg
      421 atgaaatgga gtgatggttt ttctaaaatc aattattctt tttacacagt gattggcatg
      481 attgctagtt tttacttttt gtcaaaggca atcaaaagtt tgccgatgag tctctcttat
      541 cctatctgga cagggattgg cgcagttggt tcggtcataa ttggtgtggt cttgtttcat
      601 gataagctga atctttccac ttggttcttt gtggggatgc tactcatcag tatcattggc
      661 atcaagatta ctagcggcca ctagacactt ggaataagta ctgatcagaa aaaataagcc
      721 tttttattta atattcaatt tttttatgaa aaaataagaa ataagtccaa tgacctagcg
      781 acttctccac tcatcaggtg tatgcttgta ctatctatag aaaaggagtg aatatttttg
      841 aaaagtcttt cggtccaaga attatccaag aactttggtc acaaacaagc cctcagcgat
      901 gtcagtttta gcttatcccc tggcaaaatc gttggattag taggtcctaa tggcgctgga
      961 aaaacaacga tcatgaaagc tattttagga ctgatccatt actcagatgg aacaatctgt
     1021 attggtgaaa atgagatttc tccctctagt cataaagggt tggaacaagt aggagcattg
     1081 atcgaatacc cgggtatcta tccattctta acaggctatg atcatctgaa gttattctct
     1141 gaaaccaacg atgtatctgc tatcgacact atcgtgaatc agctcaagat ggaaaagtac
     1201 attcacaaaa aagcgaaatc ctactcgttg ggaatgaaac aaaaattggg aattgctctt
     1261 gccttactga accatccgtc tatcgttatt ttagatgagc cgatgaacgg tttggatcca
     1321 caagcaacaa gagatgtgcg aaaaatcatt accgacttgg ctactcaggg cacctctttc
     1381 ttgatctcca gtcacttact cagcgagctt gaaaaaatgg tcgatgagct gatcctgatc
     1441 aatcaaggaa agatcatcaa acaatgtaca atggctgaat tgaaccaaac agcgcaagat
     1501 ttcatcgtta tctctacaac aaatgatcta gctgctcgaa cgatcttgac agaagctgga
     1561 tatactttgg aagatcctga taaaatcaag cttttaaaag aaaacgaaga attattagga
     1621 accgtgatcc agctattgtc tgcacaaaat atccatgtga cagacgtcca gcatctgacg
     1681 aatgatttgg aatcttctct attggaaatt ctggattcag taaaggggga agaagaaaaa
     1741 tgacattgct tgtaaaacaa gaattattta aattgatcaa gaaaaagtca actgctgttt
     1801 tatctgttct attagttgtt ttgctcatag gaacagcact tctagccaaa aaatacacga
     1861 cgatcattga tccagttgaa atgactgctc agctttttag tgccacatct tggattgtct
     1921 ttatcatgat tgcagctgca agcacgatca tttcgatgga agcacaatat ggtacattga
     1981 aaaatctcct ttatcgaaag tattctcgag gagaaatatt agtaagcaaa tggatcacat
     2041 tagttattta ttctgtttat ttataccttt tagctattat tgtcactgtg ttgatgaaac
     2101 tgattctttt cccttcgatt tccttcacgg agaaggtttc tactggtcaa acactgatcc
     2161 aaagcttgtt cacttatact ttgggtagtt atatcggttt atggctgatt ttaagtttag
     2221 tgttgatgat tgcttgcttt atcaatagtt ccggcgcatc catctcagca ggaatcgtct
     2281 tttattttgc ttcgtcaatc atatctggta tccttattgc actcatccaa aaatgggagt
     2341 ggatcaaatg gaatccgatc agtatgttga atctgcaaaa ccaaattgga aacgaagaga
     2401 tcatgaagca attgactcat ctttctacta atcaaatgct attcggtaat ttagcttaca
     2461 tcgttttgtt cttagcatta ggatatctcg tatttaagcg aaaaaatgta tgatgactca
     2521 tcaaaaaaga ggctgtgaaa gagacgttca gccttattgc cgattggctg tctcttaaac
     2581 accgtttatt ctgaaaaagg agctgtgaca acatttgtgt cacagctcct ttcctagtat
     2641 gtttttgaat aaacgacagg ctcacaacct ctctttatac ttcttttata cttctatacg
     2701 tgaagcatcc cagtggcaaa taaaaccatt gcaatcactc cgaccatcgt cacacctata
     2761 agtaaaatct tatctaagtg gatatgttgt acaggctcgt tttgctcctt acgtgctttg
     2821 aggtaaaaat aaatgcctgg caagaaaaga acaagaacaa gcattacttc attaaaggct
     2881 acgactagca tagcccatac ttgaaaggca cttgctaaaa gccctactaa aaattggacc
     2941 cattctttcc gctgataaga aagtttcatt tggtacaatc ctaagaaaag ccacgtgaag
     3001 aaaatagctg ctgtaccaag tgtgtaagca aagttataag cactgtcagt gaataataat
     3061 gaaagtaaaa agacattcgt acataacgtc gtcaatacga tagcataagt tggtgcccca
     3121 tttttgttga gttttcccca tgctttaggc aaaagtccgt cttcagccat gtttcccatt
     3181 gtttcaatcg gtaagatcgt ccatgagagc caaacaccaa ctaaagaaat gatcaaaccg
     3241 ccgttgatca cgactgctcc ccatttacca accatcattt caaacacata gcccatagca
     3301 ggctgcgcct tgatcgtttg cagctgattc atcgtcattg tcccatatgg aagcatggaa
     3361 accaagacgt aaataataat caagccgaat atccctagaa tcgttgcttt cccaacatct
     3421 gattgttttt ttgctcgtga acctacgaca gaggctcctt caataccaac aaatacccaa
     3481 agcatcacca tgatgcaact caatacttga ctggaaaacg ggatcggctg tccattcgca
     3541 ttcaatataa tgtcgctaga aaagttatca atgaattgtt ggatcgaaaa cgtgctgata
     3601 gctactacaa taaataaaaa taatggcact aatttacaga atgtgacgat agcattgacg
     3661 aaactagcat tttcgactcc ccgattcact agccatgcat atccccataa aaagactgta
     3721 ctcatcacga tcgatgccag attttgccca ccttcaaata ctgggaagaa atatcctaat
     3781 gcgctcatca acaatgttgc aaaagctacg ttgcctaacc agcatgtgag ccagtaaccc
     3841 caaccgctga taaaaccagc gaaacggcca aaaccttctt ctgcgtaact aaagatcccg
     3901 ccttttaaat ctggacgttt tttattgata ttattgatcg acaaggagag catcaacatt
     3961 gctgttccta cgagtaccca actgaaaata gctgggccgc cagccgatcc ggagatgtcc
     4021 gtcataatac caaatacgcc acctccaatc gctgaaccaa ttactaaggc agcaagtgga
     4081 attaaaccaa ttcctttttt ctgttgctct gtatccatgt aagcatcctc caaaataaaa
     4141 ccgcttttat caaaaagtat aacaaacctt ttttattttt acttaattaa taaaaacttt
     4201 aatagaaata taattctacc ggcttttttc tcattataca agcaaagcgc ctagtccctt
     4261 tccaaattac accaccaaaa aacttaaaaa agatgctata ctaaaaggaa agaattggag
     4321 gatgatccat gaacatttta tatatatcga tttcaggaaa tacgcgtgcc tttgccaaac
     4381 atctagcaga atatgctgaa aaaatgcatg cagaagatcc tgtcaatcct gaagttactt
     4441 taaaagaaat ccatgaaaat agtgatttta cgaaagaaac agaaccattt tttactttcg
     4501 ttcctactta tttagacggc ggaaatggtt tagataacgg tgatacagaa atcttgaccg
     4561 aaacgatgcg tgaatatctg gaacatgaag acaatcataa actttgtctt ggtgtagtcg
     4621 gtagcggaaa taaaaacttc aataatcagt attgtctaac agccaaacaa tatgcacaaa
     4681 catttggctt tcctttcttg gcagattatg aattacgggg aacaccatct gatgtgacac
     4741 gaatttatca gattttagca gaaaacaata aataataaaa aagaagaagc tgaaaaatag
     4801 atttcagttt cttctttcgt ttctattgtt tctatcatat taagctttga catccatcac
     4861 aggtgctttt gcttttgctt tacctgtttt ttccaagaca aagtttttta cacgatccat
     4921 attggttacc aagacgatca tggatgtttc tttgcctgct gctttgatcg cttctaagtc
     4981 agcttctgct acaagcgtat caggtgtcac ttgctgccct tctgttactt tcacatcaaa
     5041 tggcgtaccg cctaagtcaa ctgtgttgat tcccatatga agcaagattt ctaaaccatt
     5101 tggcgttgtg atcccaatag cgtgtttcgt tgggaagaca gagctgacta ccccagtaac
     5161 tggagaatag atatgtccgt tttctggttc tacaaagtag ccttcaccca tcatcttttg
     5221 tgcaaaaact ggatcacttg ctttatctaa agaaacaact gttccgtcag caactgatga
     5281 gaggatagtc acactttcag ctgaagtttg aggtactact ttttcaggca cttcatttac
     5341 tgtggctgtt tcagtcgcta attcttcttt ttctaaacgg tctttaggaa taccgaagaa
     5401 gaatgtagca acgaagccac cagcataagc tgccaataat ccaaggatgt agcctaacca
     5461 tttaccgtca gagatcaatg ggatcaaagc ggctcctgaa ggtccaatag cgatagcccc
     5521 gacatttccg atcattccga tcactgcccc gccgatacct ccaccgatac aagcagtaat
     5581 aaatgggcgt cctagaggta atgttactcc gtaaatcaat ggttcgccga ttcctaggat
     5641 cccgactggt aaagctcctt tgatcatttc tgttaattct ttatcttttt tgcagcgaat
     5701 ccataatgcc aatgctgcac ccacttgtcc tgcaccagcc atcgcaagaa ttggaagcaa
     5761 tagcgtacga ccagtttcat caatcatttg cacatggatt ggagtcaaaa tttgatgcag
     5821 tccgaacata accatcggca agaataatgt gcctagaacg aagccggcaa atgctccacc
     5881 aacgccaagt acccagttga tcccaccgac catgccatca gaaatgaaac ctgctaaagg
     5941 catgatgaag aaaatttcaa tcagaccgat ggcaatcaat gaaagagttg gtgtaacgat
     6001 gatatctacc gcatcaggta ctactttatg cagttttttc tcaacgattg acaatagcca
     6061 aacagcgaag atgacaccta ggatcccacc ttgtccagct gcaagagctt caccagtaaa
     6121 taagtttttc agtggtgctt ccggattcat ccctgtcagc atcacaacgg ccccgataac
     6181 tccaccaaga gttggtgttg caccaaaaac ttgtgcagca ttgatacctg tatagataac
     6241 taggtagcta aacatcccgt ttttcaagat gttcatcaca tcgatatatt gttgccatgt
     6301 tgctgcatca agtgttccgg ctgtgactaa gtttgctaaa actgcggcaa tccctgccac
     6361 gatcccagta ccgacaaatg caggaatcat tggcacaaag atattagaaa tgtcttttaa
     6421 taccgatttg attttagatg gtttttgttt cgatttttgt gccgctttca tttcttgtgc
     6481 ccgttcgttt acacggtctt ttccagatgc tgatgaattt gctgtagctg tacttacaga
     6541 agaaagtttt tctcctaatt tcacgcctgc caaatcaacc atttgctgtg ctactttgtt
     6601 cactttccca ggtccgatga tgacttgaag ctgttcatct tctactacac caagcactcc
     6661 tggaatcgct tttaattcag agacatttac aagatcatca ttgatgatct tcatacgcac
     6721 acgagtcatg cagtggacaa tgctttctac attgcccatg ccgcctgccc ctttataaat
     6781 ttgttttgcc agttcttcca aagtcagttc gttttttgcc atggtctttt ccccctcata
     6841 aggtcccttt tacaaatccg tttgcttcag ctaattttgc ttgagcttcc tctttatcac
     6901 tatttgtcaa aatcatgacg atagccagct taacattttg atctgcttct tcaaagtact
     6961 tttcagctgt ttcgtaatca gcttctgtcg cttgcatgat gattcgtttt gagcgttcga
     7021 ttagtttttc atttgttggt ttgacatcga ccatcaagtt attgaatact ttacctatac
     7081 caatcatact gatagtagaa atcatattca agataagttt ttgtgctgtt cctgacttca
     7141 atcgagtaga gcctgtcaaa aattctggac ctgcatcgac ttcgattggg aaatccgcgt
     7201 gtttactgat ttcggcttgt ttattgcaag aaatcgttcc tgtgacagca ccgatcgatt
     7261 ttgcgtaatc taatccaccg atcacatatg gtgtacgacc gctagctgcg ataccaataa
     7321 ctatatcatt ttttgtcaaa ttcagcttga ttagatcttc ttgccctagc tcataagaat
     7381 cttcggcccc ttcgacagct acagtcattg cttcttctcc accagcaatt agacctatga
     7441 ccatttccgg ttctacaccg aatgtcggta cgcattcagc agcatctaac acacctagtc
     7501 ttccacttgt tccggctccc atataaatca gacgtccacc ttttttaaag gactcaatcg
     7561 tgttagcaat cactggttcg atacttgtca gttctttttc aaccgcagcg gccactttct
     7621 gatcttcttt gttcattagt tgaacggctt gagaaatact catctcatct aatccaaaag
     7681 tttcttggtt tctgcgttcc gttgttaggt tctctaaatt gattttgctc atttttatct
     7741 tccttctttt cttataaatt gatagttcat accagctttg atttggttga tcaatggtag
     7801 ctcttctttg ataactttag ctactacatt cactttttca tccgctggca aatcatattt
     7861 ggttaattgg atctcaccca tatagcgcaa atacttttcg ttatctaaag taatagaccc
     7921 tttcatacgc gtagctgtat ttcttgctgg gataggcgga atctctcgaa aacgtgcatc
     7981 tacgcttcgt atgacatctc ttgcatcgtc ttgccgattc gtatgcttcc ctaatatata
     8041 ttcgtaaaat tcttcatcta caggttccgt atggagcaag actgtctgtt ctttttggaa
     8101 agaagcaaat tgctcctgaa cgttttctga cagacctgca tctccgatgt aaacaatatc
     8161 cgtgttactt gctaatagat ctagagcgcc tgcaagagga tggatccctc gatgttcttc
     8221 caaagttggc aatccttgat atagtgggcc tctcaactct gtatctcctg gtacaaaacc
     8281 ttggacaagg agaccttgaa ttttgagcca ttgatttttt ttctgatacc attctttgtc
     8341 cagccctgtt tcgggacgtg gataataatt atgccaagct tcaatctgtg agaaatcagc
     8401 ttctgcttct ttcaattcat cgatatcttt aggcgtgatc gtgctagcat tcaaactgat
     8461 tttcaattga tgtgaccagt cggcaatctg ctgattggaa atatgataat ccattcttaa
     8521 acctgttaca ccaattgctt tcaaaggcgt tagatcttct aatgaaaaac ccgctcgttc
     8581 caatgcctct cctgaaatat cgaccatcaa ttctagttta ttctttttcg cttcgcttcc
     8641 tagttcgatc aatcgctttt gatatgccga aacatcatct tctggtatat gcatggaagt
     8701 gaaaataccg acaaagccat tggatgccat ttggcggata tactgtttct tctcttctga
     8761 caaagaatcg ttcataaata ttgaaaagcc aaacatatta tccctctttt tctgtatccg
     8821 tcttcatttc tatgactaga gtatatatct aaagaaacaa aatttcaata cttttattaa
     8881 aataattaaa tttcatttct aaaggttgag tttatattat caaaagaatt gtcataacct
     8941 aaaaaaggca ctgctaagca aaagcagtgc cttgaaatat tttatttaaa taacacaaaa
     9001 acataattac tcataagaaa ccaattctgt attatctacc atgaatgtaa agaacccctc
     9061 attaggcatc ggatgaaaat aagcattgat cactcttatc aaggcattat ggcaaactaa
     9121 cagatagaca ttttcttcgt cttcgatgca ttctttcaat aaaggaacga tccgtgcata
     9181 cacatctaag actgactccc catttgggaa tcgtacagcg aacgctaaac gtgctttttg
     9241 aaagttctca tctttgcttg gcagtccatc ataatcccca aaatccattt ctaccaatcg
     9301 ttcatcaacg gtcagcggca agctgagacg atcagcaact gcttgtgctg tatctcttgc
     9361 tcgttgaaga ggggaatgga tgattttcgt gattggaaca ggcaaatcta ctaccttttc
     9421 tgctagtttc tgtgcctgca tctgtccttt ttcagtcagc ggcacatctg cccgtccgca
     9481 aactaatcct tgtgcgttcc aagttgtttc tccgtgtctc gttacataaa gcatgtgatt
     9541 ctctccttcg ttggcaaagt tccccttaac cataccttgt tgttcttgag attacaaggg
     9601 ttagattctc tcaagagaaa taatctcagt agatacttta tccaggaagg tttgatcatg
     9661 ttccactagt aacatggcag gtttcacgtt caagatcagt tgttctaatt gttcttgatt
     9721 gaagacatcc aaataattca atggttcatc ccatgtatat agttcagctg gctgtgataa
     9781 agatttagcc aattccactt ttttccgttg gcccatactc atctgctcga tcttgttatg
     9841 aaaaacatct ctttccatcc caagctttcg gaggttgttc aaaaatgctt ggtagtctac
     9901 ttggtttttc tctgcaaatt ccgccaacgt tcctcgattg tcttcataat tttggcttgc
     9961 ataactaatg ctcagatgtt ttggctggta tttttctcct ataaccttgc cgttgaatgc
    10021 ccccagaaga taatggataa tggatgactt tcctgcaccg ttaggacctg aaatcgccac
    10081 ccgctgatga ggctcgattg taaaatgaat tggctcgaat aacagatttt cataccctaa
    10141 ttgaagatct tctacgctta aaagtcgctt atggtgagac gcttggctat tcatcgtcaa
    10201 agaatcgata tattcgatat cttttaatag tttttctttc tcgctgatct gggtctccat
    10261 ccgattcacg attgctttgg atcgtttcat cgtccgtgca gcatcagcac ccactgctcc
    10321 tttattcact cgtctagatt cagtatcgat gaatccgact tgtttctttg ttttatctcc
    10381 ttctcgggaa cgagaccatt cggctttttc agctgctgtt ttctttagcc tactgacttc
    10441 ttttttcaat ttttcatttt gagccatttc aaattcatca cgaagttttt tctgttcttc
    10501 atagatagag aaattccctt gataaagttc cagttgactt ttttcgattg ctaaaacatg
    10561 gtccactact tcatcgataa atccccggtc atggctgacc acgatgaagc cttgtttttt
    10621 ctttttcaaa taagccgcta cttgttttct accagagata tccaaatgat tcgttggttc
    10681 atcgattaac gggaaatgag tgtcatccac aaataaaagt gccagtagga cttttgtctt
    10741 ctctcccccc gatagtgtgc tgaattctct ccataagatt tcaggatctg tttgcatcaa
    10801 ttggagctct ctttcgattt cccatatctc aaaatccgta atatcattta acacgtaata
    10861 ggttaaacgt tctttatctt ttgtctgttg cgggaaatag gcaaattctt gctgatggat
    10921 tacttgccct tgataaggta gtttgttttg tagaatattc agtaaagttg tctttcctcg
    10981 accgtttcgt ccgataagtc ctagtttcca ttgtgtgtca aaatttagat ttgcttgttc
    11041 aaataataat gtgccttggc tgtcgtagcc gaatgtcaga ttttttattt cgattttaga
    11101 cattttccat cacctcgtaa aaaaagacca gtcctatttt cagaactggc taaagaggat
    11161 gcaagggttg gatacacaca aaaagaccca tacatcgaaa aaaccaagcc aatcccgaaa
    11221 atctgcacac tgcttccttg tctccacaag gtacagtctg aatgcagctt tcatcaatcg
    11281 acttaatttt tcaatgcacg gatccctcac tttgtcctat atttgaaatt acgatagcat
    11341 aaagaaaatg attttgtcaa ctacttcggg aggatggtga aaaatctgct aagtgtctgt
    11401 cctaagattt ttgttttttc taatgtttgc ctgtgttcca ttgcttcttc taaacctatt
    11461 ggacccaaat gaatacttaa gacaattggg agaaaatcag aaagtccttc tagctcttgg
    11521 tctctacttc cgcaaatagc gattattggt tttcgatatt tttttgcaag cactgctaca
    11581 ccgtatggaa cttttccgtg gatcgtttgc tgatcgatcc gcccttctcc tgtaatgatg
    11641 acatctgctt gttcgatctt tttttccaat ccagtgattt ggctgactaa atcgaaacca
    11701 gaaaccatct tcgctcccaa caaaaaaagg gctccaccaa taccgcctgc tgctccactt
    11761 cctggaatat catctaaatc cacctcccag aaatctttta tttgctgagc aatctttcct
    11821 gcttgtttat ctaagaaagc tagcgtcgta tctattcctc ctttttgtct tccaaattgc
    11881 caagcagctc cttgttgacc tgtatatgga ttggtaacat ctgcagcgat aatcaatttt
    11941 actttagaaa caagaacgtt tcttcgatca aaatgaagtt ttttagtaga taataaagga
    12001 tttccttctg tgtacccttc taaagaagca cccaaggatt ggagtaatcc tagaccacca
    12061 tcgctacacc cgcttcctcc taaagtgaca ataatttgtt ctacttcttg aagcagtgca
    12121 tctttaataa gttctcctaa ggcataactg gaagcaagtc ggatagtttg atccgttggt
    12181 ttatcaagta gatctaaccc caatgcttgt gcagattcga tgactgcggt gttttttcct
    12241 tgccaagttg tcaataaata gctgattttt cgctgacgga ataagagatc catcccatca
    12301 atggtacgcc atgttcccct gagcgcgtaa tagattgctt ctgcagtatt ttctccgcca
    12361 tctgcaatcg gcacattgat ttttcttcct ttacgatctt cccagccatt tagaactgcc
    12421 tgatttagtt caaatgaaga agcacttcct ttaaatgaat caattgccgc caaaatatcc
    12481 atctgtatcc ctcactttct tcttaagtaa gacacttttt ttcatttttt tcaaacaaaa
    12541 caatgctatt ttcttccatt tttctatata ctaaaaggga actcattgaa taaaggagag
    12601 aaaaaacatg aaaatcgcaa tagctggggc cggagcaatg ggcagccgaa tcggattgat
    12661 gctacatcaa agcggaaatg aggtcctctt gatcgatcgt tggcctgctc acattgaagc
    12721 cattcgtaca aatgggttga ttgctgactt taacggtaaa gaagtggtcg caaaactgcc
    12781 aatctattca ccagaagaaa tcatcgaaag caacgaacac gtggacttga tcgtagcatt
    12841 aacaaaagcc aatcaattag atgatatgtt ttgttcgatc caatccatta ttactgacaa
    12901 tacttatgtt ttgtgtttac taaatggatt aggtcatgaa gatgtactgg aaaaatatgt
    12961 gccaaagaaa aatatcttat ttgggatcac gatgtggaca gctggtctag ctggtcccgg
    13021 aaaagtcaca ttgttaggtg atggagaaat cgaactagag aacttggaac cagaaggcga
    13081 agcatttaca aaaaaagttg tagaagtctt tcaagaagca aatttaaatc cgatctatag
    13141 ccataacgtt cgttattcca tttggcgaaa agcttgtgta aatggaacat tgaatggttt
    13201 atgcacgatt ttggattgta atattgctga actaggcgcg caaaaagcgg cagagagcat
    13261 ggttcgaaca atcgtatctg aatttgcttc tatcgcagca aaagaaggaa tcatactaga
    13321 tcaagaagaa gtgtaccaac atattgcttc tacttatgat cctgacaaca tcgggttgca
    13381 ttacccatct atgtaccaag acctgatcaa aaaccatcgt ttgactgaaa tcgactacat
    13441 caatggtgcc atttggcgaa aaggacaaaa atatgatatt gctacaccat attgtgcctt
    13501 tttgacccaa ttagtccatg caaaagaagg aatccttggc gcagaataaa aggctctctg
    13561 atgagaatca gctgaatcag cagctggttc tcttttttta agcccaataa aagatcaaaa
    13621 agaaaacaca actctcatga tgccataaga attgtgcttg tttgtttcag tacataatct
    13681 cttcaaaatg taggtttttt atttgttttc aacagaggag aggtcatatt cgtattgctc
    13741 ataaatagtg gaatcgttag tgataaatcg ttttgcccaa ttttctgcat cataaaattc
    13801 caatagctct gataggattg gcttaaatgg tgtatgcaga aattccggta attcatctaa
    13861 atcacgttct cgaagcaaaa aaatattttc aggacgataa aaatgataat gagcaatcgc
    13921 ttgattatcc gtcactagtt tcttttgaaa atacatggat tcaaatgtcc gcaaagtcac
    13981 tccttgctga ccctcccgca acagttccag aatcccgcgc gattcccctg tttttttcag
    14041 gtactcttca taagtaagat atccaggttg ttcattccct cgatgtggaa gaatgaaata
    14101 gttggtcgta acatctcttt ttataaattc ctgcttatag ctttctgctc gttcttttct
    14161 cccattattc gttgcaccaa aaaatagatc cgtctttggt tcgctagaag aaaaattcat
    14221 atgtttcgaa taaaatgaac tattatgttt cagcccaagt tcttttgctt ccaaaaaatc
    14281 aaaatgataa atctcatcga ttgccggttc atcttgtgcc tgcttcagca tcgcctcgtg
    14341 ttctgctttg aagtgattcc aaaaaaataa cacaattttt gcattggtaa atgggcgcac
    14401 gcgtcgagcc atctccatat caaatccata atctaaaaaa acgatcagct gataatttgc
    14461 caactgttgt ctagctgatt taatgaattc cgactgtagt aaatcttttc cggaccaaac
    14521 atctaaagag cggatggaag gcaaaaaata aagtgattta ttttttgcaa ctagcagtac
    14581 gtcatttttg ttcatctttt ccgcctcact ttctctgtca ctttttccta tgatagcctg
    14641 tttaaatagt aaaaagccag taaaacgttt cctatatttg tatgaaaatt taacatttgt
    14701 atgaaaatat gatgaaataa atcattcttc tattaaaatt cgcctaaaag ttttctgaat
    14761 agttgtaatt gtctgaaaat ttgttatcat tagtcattgt taaatattgc tatttattct
    14821 taatataaat tttgattgtg aatttaaaag aaagaggtca tttatggaag ataaaaaatt
    14881 gaaaaaacaa cttagctcca gacatatcac catgttagct ttaggtggag caatcggtgc
    14941 aggtctattt aaaggaagtg gcgaagcgat tgggatcgcc ggcccttctg tattgattgc
    15001 ttttttgatt ggtgggctga ttttatatat cgtgatgaaa ggtctgggaa aaatcgtttt
    15061 aagcggtggc gacacgcatc acggattatc tggtttgata cgcccttatc taggtgctca
    15121 ttctgctgat tttactgatt gggtctactg gtctatgtgg attatcaata tcatcgctga
    15181 agctgttgca gctgcttcct ttttgcagtt gtggttcccg cacattccag cctgggtatt
    15241 tgtttttatg ttggctgttt taactacgat catcaatctt tattcggtac ggttattcgc
    15301 agaaacagaa tattggctgg cttttgctaa aatcagtgtc attatcttac tgatcatttt
    15361 tgctacttat cttgtcggtc agcaaatgtt gggcacaggc gttttcccta cccttcagca
    15421 attgactgac catggtgggt ttactcctca tggtatgaag ggaatcatca gttctttgct
    15481 tgtagttatc tattcttacg gcggttctga actgattgct atcacggtaa gcgaagcaga
    15541 tgatcctaaa cgtgcgattc ctaaagcaat caaaggtgtc atgggaagaa tcatttcctt
    15601 ttacatcatt cctttgtttt tgcttttgat tctttttcct tggaatactt tagcaggcac
    15661 gaatgtcagt ccttttgtga tggtatttga aaaaatgaat atcccatttg cagcggatat
    15721 cgtcaatttt gtgattgtcc ttgctttgtt ctcatctatc aattcgggtg tgtatgcatc
    15781 ttcaagaatt ttgttcttcc gtttgaagga tcgaaaaggt tcctctagaa aactagctgt
    15841 tttaaacaag catcaagtac cgcaacgtgc tgttttattt tgtaccagtg ttctttatct
    15901 tggtgtagct ctttcttatt ttgtcggtga caaattattt ggctatctgg caggttccct
    15961 ttcgtataca gtattattga tctggatcat tatcagtgcg gcatcctttg tactggcact
    16021 aagaaaagga acattatggg ataaaggaat cagctttttt gcattagctg tcttaggatt
    16081 gattttcctt ggtatcctgt tcacgaactc cattggggta actgtattga ctggcctgct
    16141 ttacctgttc atttatttca gttatcaaaa aaagaatgat gcgttcgtat tgacgaatga
    16201 atgaaatcga tagaaaattt tattttattt atgtggaata gatggagcta ttgaagaaag
    16261 gagttgtgac aaaagtcttg ttgcaactcc tttttccgaa taaacagtct catgacctct
    16321 tggtaaacgg tgttcaaaaa ccctctgtgc aaaataattg attgttctcc atgctcatga
    16381 catgatagaa gaaaggaatg aaaaaaagga gtttgtttta aatgaaaaag tattggaagt
    16441 ttttgttgat gatcggtgtt tcaacggtag ttatgtttgg tatgatgtac ttgaatgtct
    16501 atgcgctcga tcatatcttc ttcagtcgta cacgagtatt catggcgttg atgatgggtg
    16561 cagtcatggc agtcatcatg ctgttgttca tgtggaaaat gtatgaaaac aaaaagataa
    16621 atgttatgat cttagccgct agtcttcttg tttttgccgg ttccttgttt atggtacgaa
    16681 gccaaactac tgtcaacgac acagcttgga tgaaggccat gattcctcat cattctatcg
    16741 ctatcctggc aagtaaacga gctgatttaa aagatcctga agtaaaaaaa ttagcggaag
    16801 aaatcattca ggcacaagaa gaagagatcg acaaaatgaa aaaaatgatc aaagaaatgg
    16861 aatgaccatt atccgtccaa ataaaaaaag aggatgttcc agaagttatc agcttcaaga
    16921 aataagaaga agctacttct ggaacgccgt gaataagggg ttgtgaaaaa gctgccctgc
    16981 atcaagtaat aagaacagcc aaacaaaaat agcttctcct atttttcgtt tggttaggct
    17041 tattaccgca gatgcaagct tttgaacacc gtttattaag aggttgtaaa atcagcgttc
    17101 tgacctgagt attagagaga caaatggaaa aatagttcct catattttgc cattttttga
    17161 gctaataccg agggtcagct gattgaacac cgtttatcga gaggttgtga aaaagtcgct
    17221 tagtttcgcg tattaagaaa aattttgtga aatggctttc cacatttttg tataaatttg
    17281 gcttaatacc gagaaactgg cttttgaata ccgtttattc gtaaaaggga ttgtgacaaa
    17341 agtcttgtca caatcccttt ttatgttttt attgttctaa ttgcgctgtc ttgcttactg
    17401 cttcttgcgg tttcacaaat ttttttgaga ttccccaacc tgtgaaggca ctgaccatcg
    17461 aaaacacagg agaaagcaaa ctgaaaaata cgaatggcaa gtaagtgagt gtaggaatac
    17521 caaatgtact tgctacgaaa cttccggcaa ttccccaagg cactaagtag ttgattactg
    17581 ttccaccatc ttctagcgtt ctacctaata cagttggatc aaatcctgct ttttcataag
    17641 tttctttaaa tgcattacca ggcagaataa cagataggaa ttgttcacca ataaaaatat
    17701 ttactccgat acttgtcatc aatgttgtaa aaatcaaatt tggtgtgttc gtcatctttc
    17761 ttgatactcg ttccatcacg gttgagatca agccaaattc gatcaacaat ccacctaatg
    17821 acaaagttag aataatcaat gacacagtag gcatcatact ttcgattcct ccgcggctaa
    17881 gcagtgaatc gatttgttgg tttccagtat ttgaaacaaa ccctgattcg atgaccccag
    17941 ccaacttcgt taaggaagtt tgtgggtttt gaataaaaat cattcctact gctacagcga
    18001 tattcaacaa aatcgttaaa attgccggca cctttttcca tgcacacagt aacattaaaa
    18061 ataaaggaat cagtgaccag attgaaatag taaagtttgc ttctagaaca tcagtcactt
    18121 cacgaataga tgaaagactt gcggctttac ttgtgtgtcc taaaatcatg aataaaaaga
    18181 aagaaacaac aaatgccggg acagttgacc acatcatatg cttgatgtgt ttgaacaaat
    18241 cggcatcaac aatagctgct gctagatttg ttgattcgga taatggagac atcttatctc
    18301 caaaaacggc acctgaaaca atagctccaa caaccagcgc tggatttaag cctagtgttg
    18361 tcccaatacc gaaaaatgca atcccaatcg ttgacataac agtaaaggca ctccctacag
    18421 cgctacctac gattgcacac acaacaaata cagaagggac aaaccactgg acactgatca
    18481 atttgaaccc aatgaccatt agagttggaa tgatccctgc ttgtatccat acagcaatca
    18541 atgaaccgac taaaataaaa ataaagattg ggatgattcc tggtttgatg ccattgatga
    18601 ttccttcgtg cagccaatct gtcgggtatc tttttatcac tgcatacaac atgaccattc
    18661 cgatcgctgc taagatcgtg atattaggtg agacctttaa tccgacaacg ccaatcgcaa
    18721 tgcctaaaac aataaccatt aatacaagtg ttgcttctaa aaaacttggt gttccttttt
    18781 ttcccatgat aatctctcct tttttagttg ttcttatgtt ctaaaaaagt taagatatcg
    18841 gattactccg ccgcaattca tccgcgattc ctcctttata aaaataaaaa gacctgtttt
    18901 aaactaagga cgaatgactc cgcggtacca ccttaattaa aaacagatct gtttttcact
    18961 ttcaaacttt tattcaaatg tatggacttt ccatgtaatt cgtcaattag gcttgatcgg
    19021 ttcacagcga ccaccgactt tctgaacaaa acacctaacc gctactgcgt gaaaagttga
    19081 cttgtacgaa gtttaccttc cataagatca tttgtcaatg aaaaagaaaa tattttttca
    19141 tttttctgaa aattattttt ataactttca cacacaagat tattttttac tagaaagtag
    19201 cgccctcttg tttttttctt gctactattt cttgatgttc ttgcttgatt ctttctaata
    19261 gctttggctc tttcaacaga tcaagagctg tattggcgag tagttctgct gcaatcgcaa
    19321 tagaagcaag gcctttgtca cttttagcag cagctttgaa ttcttctgtg tgtccggcaa
    19381 tatatgtctc agagattgct accgtaggct ggattgttgg tatgacttgg ctgacatttc
    19441 ctacatcaga agaaccgaga cttgttcttt gttccgtttc tatttcttca tctggaacac
    19501 ctgcttcttt tgcatgctgg aagaataagt catcaaaaga aggagtaacg atgacatcat
    19561 caactgcatt ttggaacaat ccaaactcaa aagtcgtgcc cgtacttatc gcagcacctc
    19621 gtacgatatt ttccactttt tcatatactt tatctaatgt ttgccgattt cctgcacgta
    19681 aatagaaacg tccagcagcg taatctggaa tgacgttggc agctactcct ccgtctgtaa
    19741 tgatcccatg gatattgaca tcttttggca attgcaagcg tagagcgttg attccattaa
    19801 acacttggat caatgcttcc aaagcattta ctcctttttc aggtgctgct gctgcatggg
    19861 aagcgacacc gtaaaatttg atgtcgactg gatcattcgc taaagattct gtcgttttcc
    19921 cataacgaaa agctggatgg acgcataacg ctacatcgac atcctcaaaa aaaccttctc
    19981 gaacaaagct gcctttagct gatccgttct cgcccccttc ttcccctggg gtgccatata
    20041 cacggatttc tccaccagtt tcatcaatga attgcttcac tatgctggcg gctaatacgg
    20101 agtaagtacc aaacaaatta tgcccgcatg catgaccaat acctggtaaa gcatcgaatt
    20161 ctgccaaaaa ggcaattgtg ggaccgggtt tattggaaat ataacgtgca tcaaaggctg
    20221 ttcggtgtcc agctacttct ttttctaccc aaaacctttc tttttctaat tgtttaatca
    20281 acgctgctga ggaataaaac tcatagttac ttacttcagg atgttcgtgg atatctagag
    20341 caagtgcttg atattctggt aaacgatatt ggattagatc cttcaattgg tttgttgaga
    20401 ttgttgttgt cataaaaaat tcctcctatg ctagtcattt ttcttatttt ctcccgaatg
    20461 tttcccatgc aggcacgctt gatccttttg atgttttctc gattactttg actgtgtctt
    20521 ctgtttgata agcgtctact attttttgat acgttttatt gttttcatct tcttttcttg
    20581 caacgatgat attcacataa ggacgtgcat ggtcatccac tggttctaag aagatggcat
    20641 cagaagcagg attaaatcca gcatctacag ctacaccgct attgatcact gaaatgtcta
    20701 catctgacaa agcacgtgct gtttgggaag catctaattc agaaatgtct agattttttc
    20761 tattctcggt aatatcagaa acagtgggcg cttgtccttt tgccttatcc actttgatca
    20821 atccagcagt ttgcaataac agaagagctc tgccgccatt tgtaacatca ttagggattg
    20881 ctacttttgc tccgtctttg atatcagcta catccttcaa agtgttagaa taaattccca
    20941 gcggtgcatt gacagtattt ccgatcgata ctaaatcagt tccgtgttct ttattgtaat
    21001 tgtctaaaaa gaattgatgt tggaaagaat tcaaatcaat cgatccatct gctaaagcca
    21061 catttggttg attgtaatct gtaaatttca cgtattccaa attaatgttc tcctttttca
    21121 aacggtcttt cacgttgtcc catacatctg tatcttctcc aataatccct agttttacga
    21181 tagtttcttt tttgtcagtt gtatctgctt ttccttggct acaccctgcc aaccaaataa
    21241 ttgctacaaa tgtaatgact acaaaacgaa taattttttt cattttttct cctccacttt
    21301 caatgtgtaa tttttttaat cagttgatta ctgataaatt gacttaaaaa aactaagatt
    21361 agaatcaata atgtagccac aatcgttaca tctgtttgga atcgattata ccctctcgat
    21421 atagcaagat tccctaatcc gcctgcacct actgctccag ccatggtagt cagtccgatc
    21481 acgttgatga tagtcaacgc cgagacccga atgataccag gcaaaccttc taacaaatac
    21541 acacgtaaaa tgataccgat tggactggtt cccatcgctt ctgccgcttc gatcacccca
    21601 ggatcaactt ctagcagtgc gttctcgatt tgacgagcaa aaaacggaac aactccaaaa
    21661 atcaatggca ctaaagctgc tttcgcacca atcgtcgttc caactaagaa acgagtagtc
    21721 aatgctaaaa gagaaagcaa gataatgaaa gggatggaac gaatgatatt gatcagctta
    21781 tcaagtattt caaatagcag acggttagct agtaatccat tagggcgtgt cactactaac
    21841 gtcacaccta agatgattcc aatcatcccg ccaatgaatg ctgtccaaaa agacatatat
    21901 agtgtttgct tcgtcgcttc gacaaattca tccggaatca aataaacatt gggcaaatat
    21961 gttttaaaga aatcagtcat tttatcttcc tccttcgatc actttgatac gactatctaa
    22021 atcgtttttt tgtaaaatat ttatttttac accagatttg atcaaataat ccaatgcttt
    22081 tttccgctgt ttggtttctc cagctaaagt aacgatcaaa ctccctaacg gcgtttcttg
    22141 taataactct acattgccat ataaaatatt cgctgttact tgatatttgc tgtacagatg
    22201 agcgattaga ggttcgttcg tctgatttcc aatataactt aattccacca gccatttatt
    22261 ggcgatctga tcggcaaaag cagaatgggc aatgatcgtt tctaatgctt ggtcaagatg
    22321 agtagctgtt cgaataaaat ctttggtcaa ttcttcttca ggattgctga agatctggat
    22381 gctgctacct ttttcaacga ttcttccatc ttccataacc gctaccttgt tgcagatttc
    22441 tttgacaact tgcatctcat gagtgatcag gacgattgtt aaacctaact ggcgattcaa
    22501 tttttttaac aatgcaagga tctgaagggt cgttttcgga tcaagtgcac tggttgcttc
    22561 atcacataat aataccttag gttcattcgc caaagctcgt gcgattgcta ctcgttgttt
    22621 ttgtcccccc gacagttgtt taggataagc agatgctttt tcttccaaac cgacaagttc
    22681 cagtagttca ttcacttttt gacgacgttc ctgtttcgat tttccagaat actttaaaga
    22741 aaaatcaacg ttgtcaaaaa tagttcggct ttccattaga ttgaaatgct gaaagatcat
    22801 accgatggcc tttctttctt ttcgcaattc tttgggagag agctgactca gattcgtctg
    22861 attgacaatg acttctcctg ctgttggttt ctgcagcaaa ttcatcacac gaactaacgt
    22921 acttttccct gcacctgagt aaccgacgat tccataaaca tcgccttttt caatcgttaa
    22981 atctacagaa tctactgcct gtatctcttt ttctttttgt ttgaaacata cagaaacatc
    23041 tcgcaattca atcattaaaa ttcctcctta tctaaaaaaa gcgtcctcaa gaaaatgaaa
    23101 atgtcatttt cttgaggacg cttttttgcg tgttaccacc tcatgttcaa gctgtgttca
    23161 cacacaactc ctcttcaagt acgatcatac tctagcaaga tatcggtcgc agccgtcatc
    23221 gccttacatc tatgctcggg atgaattgct cgatggccat tttccaatag tcgttccttg
    23281 cttcttctca gctaccgaag ctctctgtca aaggttcttc tatcgtactt accatttcaa
    23341 tgcaaataat attcaaaaaa aaagtccttt tactaacata aactatgtca gtaaaaggac
    23401 gacgtatcgc ggtaccacct tttttcggaa gtttccttcc ctcaagccca acatctcaaa
    23461 aagagagatt ggcgttcgtt ttaacggtcg aacagttccg aatttgccta cttatcgaca
    23521 aattattctc caaggccatt ttcagctttt cttacttgac tcttctcacc agccagagcc
    23581 tctctgcaaa gtttccaaac ttactttcct tttcaacgaa atcgatattt tgttgttggg
    23641 attaatctac atgaggaaaa ctgaaaagtc aagtacgaaa ttcaaattat tttttctata
    23701 tttatttttc tgttttttta tttaacattt caataacagt tcgaagatct tttccttccg
    23761 caagaagttt caatttttcc ttttcttcga cggtataatt tcggacaaaa ctggaatcag
    23821 gtatcaaaac aacgcctaat cctgctcgtg aagcagctgt tgctcctact aaagaatctt
    23881 ccactgcaaa ggctgaacta gcttctaatc ctgttcgttc caaagctgtc agataaggaa
    23941 tcggtgaagg cttgttctcc gacacttcgt ctccgaatgt aaacgtgtcc aagtaatcag
    24001 ctaatttaaa gtgatccaag atatctgttg ctcttttcgt aacagtggtc gtagcaatgc
    24061 ctagtttcat atgacgttct tttaagtctg ccaatatttc ttttgcatat ggttttaagt
    24121 ggatggctcc gcttgtcagc tgttgataaa aatattcttc acgcttcgct cggacttcct
    24181 gtactttttc agggctacct gctgctttca ctaaaatatc aaaactttgt ttccagtttt
    24241 ttccgcccca agcgtcaacg atttcttttg ggatatggat atcaaattgc tgaaatcccc
    24301 aaagccagcc atcacgatac gttttttctg tgtcaactaa caaaccatct aaatcaaata
    24361 ttgcaccttt catctataca ttaatccttt ccaaaacaat acacatagtt tagcacgact
    24421 ggtccaaaaa gaacagaagc aaagaaccag ccttctcgtt tataagagtt cttggaatcg
    24481 acggatatag attttcttca cagctgtcac aagcgccaga taagcgatga ctgtagcaaa
    24541 taaccataac cagtaattgc caggaagtgg catcaagcct aattcagcac caaaacttgt
    24601 aaaaggaagg acagaaccga caataattcc taatgtagtg ataacaaaca tggcaaatgt
    24661 tgcgttgctt tggataaatg gtactttcgg tgtacgcagt gcatggagca ctaaggtttg
    24721 tgtccatagt gattcgacaa accatcctgc atggaataat gcgatgaaaa tcactcgctg
    24781 tgaactatcc aatgtatgga aacttccgcc cactacagca ggacaaataa caaaatacat
    24841 caacaagtaa gtagtaatat caaatatcga acttgttgga ccaaagtaaa ccatgaattc
    24901 cccaatacta gatgcattcc atttttttgg ctcgtccaaa tattctttat ccatccgatc
    24961 ccatggcaag gaaatgcagg aaacatcata gatcagattc aaaaacaaaa gttgtaaagg
    25021 cagcattggt aaaaatggca ggaacgtact tgcaatcagc acggaaaaca tattgccaaa
    25081 attagaactt gctgtagcct tgatatattt catgatattc ccaaaagttt ctcgtccaga
    25141 aagaaggcct ctttccaaaa tcgtcaaatc tttttctaaa aggatcacgt ctgcagattc
    25201 tttcgcaata tccacagctg tatctacaga aataccgata tcagccactt tcattgcagg
    25261 agcgtcattg ataccatctc ctaaaaatcc gacagtatga ccatttttac gtaaagctgt
    25321 cgtcaaacgt gctttttgct gaggattcaa ttttacgaat acttcatggt cttcagctac
    25381 ttgtgctaat tcatgatcac tcatttccga gattttctca ccggtaacca attcattgat
    25441 ctctagacct acttgacggc acactgaacg tgtaactaat tcgttgtccc cagtcagaac
    25501 ttttacagct acaccatgtt ctttcagagc tttcaacgct tgtttcgttg tttcttttgg
    25561 tggatcaagg aaagcaagat aaccaatcaa aaccatgtct gactcatctt tgactgaaaa
    25621 ttctccaacg acgcttggat tggtcttttg tgcgacacca atcactcgta agccatcttc
    25681 attaagtgac tgtacttttg caagtacggc ttcttttcgt tcttttgtta atggtgtaac
    25741 agtaccgcca agatcaataa atttagaaat gttcagcatt tcttcaatcg caccttttgt
    25801 gatcatttgt gttttaccag ctggatcagc aaccacaaca ctcatacgac gacgttcaaa
    25861 atcaaaagga atttcatcca ctttttgata attgatctta tctgtatcta aagtttctgt
    25921 tgcttcatca atgatagcca catctaacaa atttttcagc cctgtttgat agtagctatt
    25981 tagatacgca tggcgcaaaa cacgatcatc ttcatttcct tcacaatcca tatgatattc
    26041 aagaatgatt ttatcttgtg tcaatgtccc cgttttatcc gtacataaca catcgattgc
    26101 accaaagttc tggatggcat tcaaattttt gatcactgtt ccttttttgg ccatcacact
    26161 tgctcctttg accaaattcg tcgtaacgat cattggaagc atttcaggtg ttagaccaac
    26221 tgctacagat agtccaaaaa gaaaagcttc tagccaatcc ccttttgtaa gcccattgat
    26281 gacgataact gctggagcca tcaatgacat aaatttgatc agtagcatgg atgttttatg
    26341 gatgcctaat tcaaagcttg attgaattgg tttttcagat agcgtttctg ctacatgacc
    26401 aaatagtgtt tcatttccaa cagaaacaat gattccttcc gcacttccac tgatgacatt
    26461 acttcccata aagacaagat tttcgtaact tgtttcgctt tgctgatcgt cttcacatac
    26521 tttcgcatgc ttttctactg gatagctttc ccctgtcaat gctgcttggg aaatgaataa
    26581 atcctttgcg gacaataaac gcatatccgc tggtatcata tcccctgctg aaagccgaac
    26641 aagatctcca cagacgattt cttccatagg aatctctgtg tactttccat tacgttttac
    26701 agctgccgtt actttcacaa gtgatttcag tttttcagcc gcttgattgg aacgtacgga
    26761 ctgaatcaac gtcattgtcc cgctgacgat aaccatagtg aagataatgg ctacaccgaa
    26821 aagatcttta tctccaggag ctgctaatac atattccgtg atgaatgaaa taattcctaa
    26881 tgcgatcaac actaatgtaa atggcgtgat gtatgctttc aatatttcta caatgaatgg
    26941 tgtttttttg ccatatgaaa tcgcattttc accgtaattt tctcttgaac gttcgatttg
    27001 ttcttctgtc aatccatttg ctgatgcatg gaatagttca aataattctt gtttcgtttt
    27061 tccagcaaaa gctgcataga ttttttgtgt atctttcttt ttttccatct caatctctcc
    27121 tttgtgtcca aaagacagct gttctcgctg cttttggtct ttagctattc atcggatcga
    27181 tagagaaaaa actgaaaggg gctgagaaca gatagataat agccctggcc tttcctctag
    27241 atccttgttt ttcgaatgac tgtcatccat ttgttctcat ctcctttact aagtaatttt
    27301 aattcatgac ttccaatcat ttttcgaata gttatttttc caattagacc acctcctttt
    27361 tatttttcac cgatggggat ttgcggcaac aaaaaaacct ctcgccaaat actcagcgaa
    27421 aggtttgaaa aagcatatta aaaaaagctt ctcagatctc atcgttgagt tttgacactg
    27481 tacaacgtaa aaagcagata cctgctttta gctaccttat gaaaagcctt atctcgacaa
    27541 ttcctgttct acccattggc gtctctcgac gtttttgggc agtagcctat gtttgtatag
    27601 gagcctcacc taacaaatct tattcggttt tccttcgtta atataacaac taaaaaagag
    27661 gatgtcaaat caattttcca tgtttttcat acttttttta aaatacacac gttatattcc
    27721 tgtctcacgc ggttttattt gtctcatttt ccacctcccg ctataataga aatatagtag
    27781 gaaacgaggt gctgttatgg aaagaacact tgttattatc aagccagacg gcgtgagacg
    27841 ccatttagtc ggaagcatca tccaacgttt tgaagcaaag ggactggcca ttgctgaaat
    27901 gaagtttgaa actatgactc ctgaattagc aaaagaacat tatcagcact tgactgagcg
    27961 cagttttttt gatgaactga tcgattacat gacttccggt cctgtcgttt atcttgtgct
    28021 ggtcggcgag gaagtgatcg atatcgtccg taagatggtt ggagcaacaa aagctgccga
    28081 tgctgttcca ggtacgatcc gaggagacta cgcgctgccg gggactgaaa acatcattca
    28141 cgcttctgat tcccgtgatg ctgcggtaaa agagatcgcc cgctttttcc cgccattcga
    28201 tataaacaaa ccgtctgaaa aagcggcgaa gtgatacaaa ttctaaactg ctggtctagt
    28261 tgctgggttc agcaatggcc agcagttttt tctctaagga cctccgttga ttctttccta
    28321 acatatattc gagcactgtg aagaattctt ggtagtcttt cgtcttttct tcccctaaag
    28381 cagcagaaat caatctttga taataatctg ccatcaatgt ttcatagatc actgtacttc
    28441 tttctttatg tgctaagctc cgtttcatat cttcattcac actacgtgcc tttttcaacc
    28501 gaccattttc tatataaaac gaagtcaggt tcattaaaaa agtaaagaga tactgctcat
    28561 gattgttcaa ctgtgctaat cgtcgcattc tttttttgat tttcaaataa ttgttctcaa
    28621 tgaaccgatc actgaatatc caaggaaaat cagctagaat agatagttca aattgaccat
    28681 agtctggtat ctgcatcaag tattcttcaa tgattcggca ttcataacgt aaaaattcaa
    28741 attctgtctc agatacttct gttttttcga ccatgtgaag attcaaaacc aaataataca
    28801 gatcttctgt tttcttaaaa tgcctacggt tattgctgat ctctttataa ggatcaaagc
    28861 tgtcttcttg tacccgatct agcattcgtc tttttttctt taactttatt ggttcatcca
    28921 gctggtttcg catccattca tattctgcaa gtgtcatgtt cattcgctct aaaaatgatc
    28981 tcaatgttac aaatgacatt tttttcactg aatttgtctc aatcccgact agagaagtct
    29041 taaaacagat tccttccgct aattgggact gtgtcaactt cttgttgact cgaagtttct
    29101 tgatcaattc accatcatac ataatagttc tcctttttaa tttaaatttt tccacattca
    29161 ataaatagat acgttaaaaa ggaaaaatac acgaaaaaaa taaaaaaatg tttttttcat
    29221 aaaaaatacc aaccttcaag cattagaatg ttgtctaact actgaaggtt ggtttgattg
    29281 tttattgatt atttttttgc tgttcaacgt ctgttcttgt tactggatat aagttggttg
    29341 ttgttttatt accatttttt gaaaagacac ttgtcgaatg ttcgcctttt tgcttttcga
    29401 ttgcttcatt acgttccgct tcatttttat agtcatattt tttcggatca atcgtctcaa
    29461 atccgtttgg cacataaaac cgcaagagat tttcctgatt cagcttatct gaaagactta
    29521 acgcagtctt gactttttcc tgatctttgg agatttcctc atcgattcct gctgctttcg
    29581 tatcgatgat ttctcctgtc tgattattgt aagcttttcc tcctaggacg gtgtatttgg
    29641 gtgtcacaaa gttcccattt cgaaaagcaa ctacttgatc atgctgagaa gataataagt
    29701 ctgtaccaaa atgaagataa tctttatcat cgattcccaa tagatgtagt aatgtcggca
    29761 aaacatcgat ttctccaccg tattcatgat tgactgtccc tttagtataa ccaggcatat
    29821 ggatcatcaa tggcactcgt tgcatctgtg cgttgtcaaa ttcatcccat gtatctggat
    29881 ctttaccaag agcgcttgct aaatctttat tatctgtatt cgagatccca aaatgatccc
    29941 cgtaaatcac aaagattgaa ttttggtaaa taccagattt tttcaaatac gtaaaaaatt
    30001 gttccaatga ttgatccaga taatgtgccg ttcgaacata attatctact gtgctattgc
    30061 cagtattcaa agatggaaag tcgaaattct tgtcatccgt ataataaggc gtgtggttgg
    30121 ttacggaaag aaatttcgtg taaaacggct gttgtagatg ctcaagatat tgggcagatt
    30181 ctctgaataa atctttatct aatatcccat aacctaacgt ttcgtcggat tcatcaaaat
    30241 aagatcgatc aaagaaattg tcataaccta agtttttata tacatgatct ctattccaaa
    30301 aactagctac attcccatgg aaaacagcgc ttgtatagcc ttgcttttgt ccaaggatcg
    30361 ctggtgcggc ttgaaagaca ttgtctgacc caagttctgt gaataatgag ccttgaggta
    30421 aaccaaatgt gccagtttcc aacatatttt cagcatcact tgtctttccc tgtcctactt
    30481 catgaaagaa attgtcaaag ctgatcgttg cttgattctg gaaaatacta tttaaaaacg
    30541 gtgtgacttc ttgtccgtct accttcatat taatcaaaaa ctgctggaaa ctttctaaat
    30601 ggagaacgat cacatttttc cctttagcga tcccaaatgt ttcgggattt ggctctgcat
    30661 aatgtttctt tgtataatct aaaacttcat caatcccatt actgcttgca tgcgcgcgaa
    30721 cactgctagt cattcctgtt ttgattccgt cataaatagt atacgcatcg attcctagat
    30781 atttgacaat atatgaacgg tcaaatgttc gctgcagcaa ttgtggacgg ttggcttcgc
    30841 tcagcgctag atttcctgaa aaaataagcc ctgaaagaca ggtaacagcg atagccatcg
    30901 gtttgccgac agggttgctt ttgataggta ttttcttgac cttcaaccaa accagtagcc
    30961 caatgaacac agcgatatcc agccagtaga tgacatcatg gggcttcatc aaagaaaagg
    31021 agcttcctga caatccttgc gacactttcg aaaagcctaa tacagatttg actgtaatga
    31081 aatctgtgaa ttcccgataa aaaataatat tcaagtacaa caaaagcgta tttaaaatat
    31141 caatgatcaa aagaatagct aacgctggct ttggcttttt aatatacaaa gaaaggccaa
    31201 aaaacaataa ggtagtcgca attgggttga tccacaagat aaagtactgg atcgtacctt
    31261 ccacacctaa tgaaaaatct gcatagtagg caatgatcgt cttcagccag aaaaagaaaa
    31321 cagctgtcaa aacgattgct aaacgatttt ggcacaattt tttaagttgt gtcacgtata
    31381 tcacttctct ctattcacat tttttcttat tttgctcctg aaaaatgaaa atttcctaaa
    31441 aaagttgtta tcttaagatt tttttattct ttctaaagat tattccgtcc acttttcaca
    31501 gcataaaacc gctgcgatgt aagcgattaa actaatgaag agataaaagt cttgtggtga
    31561 ccgcttcact ccacggtatt caaaagccag tttctcggta ttcgtacaaa aaaagaggct
    31621 gtgacaaaat tcttgtcgca gctccttatt tcttgatgca ggacagcttt ttcacaactt
    31681 cttccttaaa cggtgttctc ctgccaactc ctcggccaca agtcacaata tttataaaac
    31741 atgagaagct gttttttgaa attgttcctt gtggctcaga gttaaacggc aggctcaaga
    31801 cctcttgata cacggtgttc aatcagctga ccctcggtat tagattatgt caggacgcta
    31861 attttacaac ctcttggtaa acggtgttca aaagcttgca tctgcggtaa taagcccaac
    31921 caaacgaaaa atataaggag ctattttttg aaattgctcc ttgtggctca gagttaaacg
    31981 gcaggctcaa gacctcttga ta
//
LOCUS       DAZKOU010000026        31430 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000026 DAZKOU010000000
VERSION     DAZKOU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.026"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(742..1083)
                     /locus_tag="ACG827_001116"
     CDS             complement(742..1083)
                     /locus_tag="ACG827_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0483274.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1083..1313)
                     /locus_tag="ACG827_001117"
     CDS             complement(1083..1313)
                     /locus_tag="ACG827_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0483275.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1600..1938
                     /locus_tag="ACG827_001118"
     CDS             1600..1938
                     /locus_tag="ACG827_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483276.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2143..2705)
                     /locus_tag="ACG827_001119"
                     /pseudo
     CDS             complement(2143..2705)
                     /locus_tag="ACG827_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3437..4018)
                     /locus_tag="ACG827_001120"
     CDS             complement(3437..4018)
                     /locus_tag="ACG827_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0483277.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4201..4827)
                     /locus_tag="ACG827_001121"
     CDS             complement(4201..4827)
                     /locus_tag="ACG827_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0483278.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4849..6180)
                     /locus_tag="ACG827_001122"
     CDS             complement(4849..6180)
                     /locus_tag="ACG827_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0483279.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6335..6736)
                     /locus_tag="ACG827_001123"
     CDS             complement(6335..6736)
                     /locus_tag="ACG827_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0483280.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6822..7028)
                     /locus_tag="ACG827_001124"
     CDS             complement(6822..7028)
                     /locus_tag="ACG827_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483281.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7123..8625)
                     /locus_tag="ACG827_001125"
     CDS             complement(7123..8625)
                     /locus_tag="ACG827_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0483282.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8643..9098)
                     /gene="argR"
                     /locus_tag="ACG827_001126"
     CDS             complement(8643..9098)
                     /gene="argR"
                     /locus_tag="ACG827_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0483283.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9196..9813)
                     /locus_tag="ACG827_001127"
     CDS             complement(9196..9813)
                     /locus_tag="ACG827_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0483284.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10034..11407
                     /locus_tag="ACG827_001128"
     CDS             10034..11407
                     /locus_tag="ACG827_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0483285.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11482..12594
                     /locus_tag="ACG827_001129"
     CDS             11482..12594
                     /locus_tag="ACG827_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0483286.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12666..14303)
                     /locus_tag="ACG827_001130"
     CDS             complement(12666..14303)
                     /locus_tag="ACG827_001130"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0483287.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14471..15079)
                     /locus_tag="ACG827_001131"
     CDS             complement(14471..15079)
                     /locus_tag="ACG827_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0483288.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15463..16614)
                     /gene="queG"
                     /locus_tag="ACG827_001132"
     CDS             complement(15463..16614)
                     /gene="queG"
                     /locus_tag="ACG827_001132"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0483289.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16633..17385)
                     /locus_tag="ACG827_001133"
     CDS             complement(16633..17385)
                     /locus_tag="ACG827_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483290.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17526..18476)
                     /locus_tag="ACG827_001134"
     CDS             complement(17526..18476)
                     /locus_tag="ACG827_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0483291.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18477..19316)
                     /locus_tag="ACG827_001135"
     CDS             complement(18477..19316)
                     /locus_tag="ACG827_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0483292.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19313..20059)
                     /locus_tag="ACG827_001136"
     CDS             complement(19313..20059)
                     /locus_tag="ACG827_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0483293.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20297..21667
                     /locus_tag="ACG827_001137"
     CDS             20297..21667
                     /locus_tag="ACG827_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0483294.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21722..22618)
                     /locus_tag="ACG827_001138"
     CDS             complement(21722..22618)
                     /locus_tag="ACG827_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0483295.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(22732..22902)
                     /locus_tag="ACG827_001139"
     CDS             complement(22732..22902)
                     /locus_tag="ACG827_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483296.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(22899..23576)
                     /locus_tag="ACG827_001140"
     CDS             complement(22899..23576)
                     /locus_tag="ACG827_001140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0483297.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23667..24572)
                     /locus_tag="ACG827_001141"
     CDS             complement(23667..24572)
                     /locus_tag="ACG827_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0483298.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            24747..26201
                     /locus_tag="ACG827_001142"
     CDS             24747..26201
                     /locus_tag="ACG827_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0483299.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            26217..27248
                     /locus_tag="ACG827_001143"
     CDS             26217..27248
                     /locus_tag="ACG827_001143"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0483300.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27255..28442
                     /locus_tag="ACG827_001144"
     CDS             27255..28442
                     /locus_tag="ACG827_001144"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0483301.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28598..29980
                     /locus_tag="ACG827_001145"
     CDS             28598..29980
                     /locus_tag="ACG827_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0483302.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30017..30337
                     /locus_tag="ACG827_001146"
     CDS             30017..30337
                     /locus_tag="ACG827_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483303.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(30537..31205)
                     /locus_tag="ACG827_001147"
     CDS             complement(30537..31205)
                     /locus_tag="ACG827_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0483304.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8859 a         6379 c         5684 g        10508 t
ORIGIN      
        1 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
       61 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      121 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      181 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      241 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      301 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      361 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      421 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      481 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      541 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      601 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      661 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      721 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      781 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      841 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
      901 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
      961 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1021 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1081 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1141 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1201 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1261 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1321 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1381 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1441 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1501 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1561 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1621 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1681 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1741 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1801 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1861 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     1921 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     1981 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2041 tatatgcagc ttatgagcat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2101 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2161 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2221 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2281 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2341 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2401 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2461 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2521 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2581 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
     2641 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2701 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2761 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2821 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     2881 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     2941 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     3001 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     3061 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     3121 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
     3181 acctctccac taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
     3241 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
     3301 caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac
     3361 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
     3421 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
     3481 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
     3541 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt
     3601 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
     3661 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
     3721 agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
     3781 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
     3841 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
     3901 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
     3961 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
     4021 atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct
     4081 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
     4141 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
     4201 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
     4261 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
     4321 cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc
     4381 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
     4441 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
     4501 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
     4561 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
     4621 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
     4681 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
     4741 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
     4801 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
     4861 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
     4921 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
     4981 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
     5041 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
     5101 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
     5161 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
     5221 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
     5281 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
     5341 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
     5401 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
     5461 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
     5521 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
     5581 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
     5641 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
     5701 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
     5761 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
     5821 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
     5881 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
     5941 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
     6001 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
     6061 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
     6121 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
     6181 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
     6241 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
     6301 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
     6361 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
     6421 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
     6481 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
     6541 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
     6601 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
     6661 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
     6721 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
     6781 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
     6841 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
     6901 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
     6961 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
     7021 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
     7081 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
     7141 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
     7201 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
     7261 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
     7321 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
     7381 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
     7441 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
     7501 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
     7561 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
     7621 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
     7681 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
     7741 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
     7801 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
     7861 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
     7921 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
     7981 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
     8041 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
     8101 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
     8161 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
     8221 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
     8281 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
     8341 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
     8401 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
     8461 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
     8521 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
     8581 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
     8641 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
     8701 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
     8761 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
     8821 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
     8881 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
     8941 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
     9001 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
     9061 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
     9121 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
     9181 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
     9241 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
     9301 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata
     9361 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
     9421 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
     9481 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
     9541 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
     9601 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
     9661 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
     9721 gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt tcatactata
     9781 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
     9841 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
     9901 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
     9961 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
    10021 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
    10081 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
    10141 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
    10201 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
    10261 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
    10321 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
    10381 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
    10441 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
    10501 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgtt
    10561 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
    10621 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
    10681 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
    10741 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
    10801 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
    10861 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
    10921 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
    10981 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
    11041 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
    11101 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
    11161 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
    11221 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
    11281 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
    11341 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
    11401 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
    11461 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
    11521 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
    11581 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
    11641 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
    11701 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
    11761 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
    11821 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
    11881 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
    11941 cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
    12001 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
    12061 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
    12121 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
    12181 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
    12241 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
    12301 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
    12361 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc
    12421 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
    12481 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg tcattggcct
    12541 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
    12601 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
    12661 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag
    12721 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc
    12781 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
    12841 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
    12901 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
    12961 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
    13021 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
    13081 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
    13141 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
    13201 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
    13261 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
    13321 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
    13381 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    13441 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    13501 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    13561 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    13621 cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct
    13681 agcacagccg ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca
    13741 tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt
    13801 tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa
    13861 ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg
    13921 ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt
    13981 aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt
    14041 gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa
    14101 tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt
    14161 gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc gtaaaatgtc
    14221 gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat
    14281 tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct
    14341 ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta
    14401 tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc
    14461 ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac
    14521 cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc
    14581 cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt
    14641 atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg
    14701 ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat
    14761 gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg
    14821 ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga
    14881 tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt
    14941 tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag
    15001 catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa
    15061 ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc
    15121 tattaacttt atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa
    15181 aataacgaat tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat
    15241 aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa
    15301 aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg
    15361 atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca
    15421 acaattactg gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg
    15481 caataatttc tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa
    15541 tatttcatct aatacatcaa gccatttttc cggttctttt gttcctagac gtccaatagc
    15601 ccaagctgct gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg
    15661 caatgccgtt cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt
    15721 cttcccacgc catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat
    15781 cgaaagcatt ggctttaatt ttggatatat ttcgtctact tttggttcca tctcttcatg
    15841 aaaatgaaaa tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata
    15901 aatgacattt cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg
    15961 ataagacaaa catcgctggg cattcattct tccgtcacca agcaatgctt ttgtcggaca
    16021 agcagtcacg cagcgcatac agtcaccgca accaaattct cctggtttat caggctcaaa
    16081 tacaatatta gtaacgatct caccaagata gacaaatgaa ccgaactctt cagtgatcaa
    16141 caatccgttt cgtccaataa atcctaaacc agctcgctgt gctacagcga catcgatcag
    16201 ttctccagta tctacttgcg gagctagacg ccattcctct tctttttgcc atttttctgc
    16261 tcttgtttgg atgaattcga tcaatcggtt caaacggtct ctgagtatat catggtaatc
    16321 gatcccccaa gaagcacgag caaactgccc gcgtttttca tccctaggca cttcttcatg
    16381 gatcctagta ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat caaatgtcat
    16441 ttctggataa agacgctcat cgatatttgg atgttcaaag ccagaagtat ggccagctgc
    16501 tttttgttct tccaacgatt cttttaatga atcaaaaggt tccgccgtgg tgaacccgat
    16561 tttgtcaatt cctaatcgtt tgctttcttg tatgatttct tcttttaatg tcatggtctt
    16621 cctcgcttgc tgttaacggc tgtataaccc ataatatagt ttttcttttt tcagctggac
    16681 aaactggatc tgctgatcgg caatcttctt ttcttccatc catttttttc ttgcagcaaa
    16741 tacttcccaa taatttgttg aaattaattt tcttttgtta tctaatgcat taaaaaaatt
    16801 caatgaaact ggttttgtac ttaacagttc atggagcctt acctcttccc atggcgaaaa
    16861 ctcagacaga tcgcgggaat acaatacttc ttcatagaga tttttttgct gtgtgtacgt
    16921 gaatgtttgc gggattttcc caggcctagc aatttctact agacgattat aaaacatatt
    16981 cattaatttg ctgttggcaa taaattgact tttataatag gccgactgga tctttttgat
    17041 tactccttct gctttgatga ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc
    17101 gttttgttta actaatacgc caggtgtgaa gccgactgga gaaaggaaca caaaataacc
    17161 gataccggta ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag
    17221 ctcttcatct ggttctgttg tctcattttg atttgctatg aataatttag ctaattctgc
    17281 ttcaagaaat tgaaggtctt gatccttgtc ggtattttcc tgctggctga tcaatcgaaa
    17341 gacttcttct ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca caccttcctt
    17401 ccttactcta actataacgg aatttcttca aaaaaggtat ctgtacgacg aaaaacagca
    17461 taattttttt tcatatctct tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc
    17521 acttttcagc tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat attccatttc
    17581 atcatctgat agtaagaatc tccttgttct ccttcttttg ctatggaatc agtgaacagt
    17641 ttatcataga ttttcaaacc cgtttctttt gctacccgtt ccatgcttct tgagtctaca
    17701 ctggtttcca cgaataaaac cggtacttct tttgctctta tctgatcaat gatcgctttc
    17761 atttgatcag gcgtaccttg actttctgta ttgatctccc atatataagc tgctggcaaa
    17821 tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg ttactaataa ttttttgttc
    17881 tcttcgatag aagcaaaact ttccttcgct tgtgtatcta attcttttag tttttccaca
    17941 tatgcttctg catttttttt atagagtgtc gcattttctg catctttttc agagaatata
    18001 cgtgcgattt cctccacata ttttattccg ttagataggt ctagccaagc atggggatct
    18061 gcttttgtat gttcttcacc gctagttaaa taaagaggtt ctacattttt gctaactgca
    18121 aaataatctt ttccttcttc ttttttagcc gtttccatca agttatcgaa ccagctgtta
    18181 cctgtttcaa gattcaaacc gttgtataaa ataacatctg catcgcttgc ctttttgatg
    18241 tcttctggta gtacttcata ttcatgcgga tctgttccga ctggtacgat actgtggata
    18301 tctatttgat ctgttccgac ctcttttgcc atgtccgcca agatcgaatt ggtagccacg
    18361 actgacaact tttctttact tccttcatcc gcttggtcag aagcctgctt tccgcaaccg
    18421 gctaatacca agaaactaac tgccaagatt aaaaagagtg atttttttgt tttcatctat
    18481 ctgcttcctt tctgaaataa aattcctttt gttggagaaa ataaaaaagc aaggataaaa
    18541 aatgcggtac atgctaacac gatcgtagct cctgatggcc aattgtaact ataactgaaa
    18601 aacacaccga caactgcact tagcataccg attcctgcag ctgtaatgat cattttcagt
    18661 aaatggttcg ttagcaagta agccgtggcg gctggtgtga tcaacatggc aatcaccaag
    18721 attgttccta ctgtctgtaa actgactaca gccactaatg tcaaaaagaa catcaaaaga
    18781 taatggatca gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc gaatgatgtg
    18841 atcttcaatt ctttataaaa gaaaaagaca aatatcaaga cgattccact aacgagtgct
    18901 gtcagcaaca aatcgctttc cctaacagct aagacattcc caaataaaat atgatacaaa
    18961 tctgtcgcac tctgggcata tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag
    19021 acaattccaa tcgctgtatc atttttcaat cggctccgct gagtcacgaa accaatcact
    19081 gctgctgtta gcatcccaaa agcagtggct ccaaagatgt aactaaaacc aaacatataa
    19141 gaaatggcta cacctggaag aactgcgtga gagatagcgt ctcccatcaa agacatccct
    19201 cgtaaaataa taaaagaacc gatgactcca gaagcaagtc ctacaagcat ggaagtgacc
    19261 aaagcgtatt gcaggaattg ataattcatt aatccatcaa taaaggattg gatcattttt
    19321 tctctcctcc ttttactaaa tgtctgatga tctcaccata ggccaattga aggttcttcg
    19381 atgtaaagac ttcttgtact ggaccggaag cgatcagctg tttatttaaa agaataacct
    19441 catcgaaata ttcttctact ttatgaagat catgatggac gatcaaaatc gtttttcctg
    19501 aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc acttaacgca tcaatcccta
    19561 cgaatggttc atctaaaaag atccattctg ctccttgggc tagagctctt gcaataaaaa
    19621 ctctctgaag ctgtccaccc gatagttcgc tgatctgtct tttggcatat tcttccatcc
    19681 ctactttttt caaagcttgt cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa
    19741 ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa
    19801 gatccaattc acttcgctgt tccacgtagg ctattttttt acggactgag tttaccgact
    19861 gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa gatttcataa
    19921 atgttgattt tcctgcaccg tttggtccga taataccggt aattttttgt ggatttatcg
    19981 tcaagttgat agttttcaaa acagttttcc cttgataagc ggctgaaagg tcttgaatta
    20041 caatggccgt acgttccaaa tcttacactc ctttttaggt ttacctaact ttttatttaa
    20101 atatagttta actaaaatgg aaagcatttt caagtagaat gatgaaaaag tttttttcga
    20161 attttcctcg cattttatga aaattttctg tagaatcaca tcaagagttc aataaggcta
    20221 acctaataat gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt gactcaacaa
    20281 cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga agtcttagtg cccgtcatat
    20341 ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac
    20401 catacgttgg acagggccct cagtgatggg agcctatgcg attgcaggat tctttttgta
    20461 tttgattatg cgtgctttag gcgaaatgct ttacgttgat ccttcgactg gttcttttgc
    20521 aaattacgcg agcgagtaca tccatcctgt ggctggctac ttgaccgctt ggagtaatat
    20581 ttttcaatat attgtcgtag gaataagtga agtgattgct gtaggatctt atatgaatta
    20641 ttggtggcct gatttgccgg caattattcc cggaatcatt gtcgtcttgt ttcttatgct
    20701 ggcaaatctt atttctgtca aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa
    20761 agtcataacg atcattttga tgatcattgc aggtcttggg gtaatcttat ttggatttgg
    20821 taatcatggt caggcagtag ggatttcaaa tctttggaaa aatggtggat tcttcactgg
    20881 aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt gcttcctatc aaggaatcga
    20941 actgatcgga atgactgctg gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt
    21001 ccagtccact atcggtcgaa tcttgatttt ttatattggt gctattttta tcattgtgag
    21061 tatctatccg tggaatcaat tgagtgagat tggttcacct tttgtacaaa ctttttctaa
    21121 agtcggcatt acatttgctg caggactgat taattttgtc gtgattacag cggcattatc
    21181 aggttgcaat tcagggattt tcagcgcaag tcgtatggtc tatacacttg ccataaatgg
    21241 aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt
    21301 agcaatctca ggtggaatct taattggtct gatcttaaat tatcttctac caatgttcat
    21361 caaccagtca gaaaatattt tcgttttcgt atacagctca agtattttgc ctggtatggt
    21421 tccttgggta gtgattttga tcagcgagat tcgcttcaaa aagaagcacg cagataaaat
    21481 gcatgatcat ccttttaaaa tgccactatc tccttatacg aattatgtga cacttgcttt
    21541 tttagctatc gtattgattt tcatgtttat caatcctgaa acgagaatct cattgattat
    21601 tggactcgtt ttcttgcttt acatgacgat ccattacttt gtacgcgaaa aaagaaccgg
    21661 aaaataaaaa aagaacgaag cttcacctta gtggaagctt cgttcttttt tattgctttt
    21721 atcaattcaa ttttagcgtc tcaccgggag tcaggaaata attctccatg ctcatcccat
    21781 tcagcgacaa gatcgtttct actggtacgc cagttcgttc ggctatttga tttggacctt
    21841 ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag
    21901 aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc agaagcttct accgactctg
    21961 ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg
    22021 cgctagtctg caaataaata tcggaaatct tagcgacatg attcgtataa ttagccgata
    22081 attgattgat caaagtatct aagtctacac cgtctttatt ttctgctgcc tgatacgctg
    22141 cacgtaattt tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag
    22201 caactgctga taaaccagct attccctctt gatttccctg aatttctgtc tggtattctt
    22261 caatcaatgc aggcgtcttt tcttcgatct ttttcgaata atcatttacg attgcttgaa
    22321 gttgctctgg caaaccattt gctgtttttt cttcagaagt ttgtgtctct tttgttttag
    22381 ttgatgactc acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg
    22441 tacttttcgc ttctgacgaa ttttttccat gaaaatatag acccgcaact cctcctgcta
    22501 tgatcaaaaa cacggcaact gctgccccaa tcatgatcat ccgcttacga gctgcctgtt
    22561 tttgctgtct tcttcgggtc atccgaacat tttctcccca ctcattttca tcatacattt
    22621 ttttcaccac caatttttat gtataatagc gttatcaaac gcttcatatt cttattgtag
    22681 caaataattg tcgctgtgca catttttatc tttgttattt ataagaaaat ttcaccagtt
    22741 gagatgattc caagattgtc cgtcgatttc tccttgcagc tgcattgttc gttcgtctat
    22801 ctcagccatc agtttcttta tttcatataa gagcgcacgg tcttgatatt ctgatttttc
    22861 gcttaattca tctaattgtt ggatcagcca atcatttgtc ataatgaagt tcctttcatt
    22921 tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag tgcttctacg
    22981 atatatgctt gtttttcaga ttcttcttcc tgcattttct gattttcctg tactctttga
    23041 ataaaccagt caatccgctg agtcagcgat ggtttgcctt ccaatgagag atactgctga
    23101 taagcaatta gcttctccca acgtttttcc tgggataaat acataaattg tgctaaaacg
    23161 aatggcggaa gataggtcat atggaattta tttgcgattc gctgatacgg cctcaaaaat
    23221 tcggaaatcg tatattcttc ttttcctcca gcttggtatt ctttttccgc cacacctgtt
    23281 gtcaatacga ttcctagttc tttccctttt aatacatgtg ctgctcgttc caagacatta
    23341 tcttgccaaa tttttaaatg agcaggagaa ctataccagt aaaacggaaa ctggaaaatg
    23401 atccgatcat gctgggccaa gagctgtagt tcagtctcta tatctatttt tccgtctgga
    23461 taacttgatt ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat
    23521 tgctgattta aagattcttc gatcgttgga tggctaatga tgataagtgt acgcatccct
    23581 tttcctcctt tttagtcttc gctttacagt gtagcataac cgccacaagt ttcttttgcc
    23641 gatgcttgtc attttaacca gactaatcag gttgtccaag aaactgcggt ttgtttttcc
    23701 aactgttttt tcaaggtatc caaagcaata ttcttaaaat tttcgttttt catacgttta
    23761 cgatagttcg tagccgtttc tccggtactc tttttgaaca ttcggctaaa tacgactaga
    23821 ctattgaacc ctacttgata ggcaatactt tccacggatt catccgtcag agaaagaaga
    23881 aacgcccctc gattgatccg taccccgatc aaatattcct tgatactggt acccatatga
    23941 tttttgaata ctcgcgacaa atggctccga ttgaccgcta aatgctgggc gatttcctca
    24001 acagacaatt tttcagcata atgtgcctcg atgtatttga ctgtttcaac agctaaagag
    24061 gaatgaacct tttgtacacc accgctgacc tttccgccat cttttaaaag gactgataaa
    24121 aaacggtagg ttaatgctgt taactgtaat tcattttcca aggtttcttc cgtatctttc
    24181 atgcattcta aaataatgtc gatatatttt tgtatgtcag aagagaccat cgtgtattgg
    24241 tcttctctga aaagcgactg ccgaatgatt tcttctgcag cactcccacc aaatgaaagc
    24301 cagcaataca tccacggatc ttctccatct gataagtaaa acgtcgagtc tcctggacga
    24361 atcaagaaca gatcaccttt tttcaattgg aattgttgat ttttcacatg ataagatcct
    24421 ttgccttcca tcacgacatg caggatgtaa tcattgcgaa tagcaggacc aaaagaatga
    24481 aacggcatcg ttttagagaa accacaaaaa tcaaaataca attctttttc tacttgttga
    24541 tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc ctccttactc ctttagtata
    24601 atgcaacaaa aagttaaata aaagtcacag aaatatatta aaaaggaagc gatttcttgt
    24661 taagtttaag tcattaaaag atctttgtta ttcgttgtaa ccggtttact tagtattata
    24721 tattgaacag gagaggaata cagattatga aaagcaaaat gatccaacgt gtgtcctacg
    24781 ctttaggcgc ttttggacac gacacattct atgttacatt aagcacttat tttatggtat
    24841 tcgtgacaag ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa atgattgcta
    24901 cagttacaag cttagttgta ggtatccgct tagttgaaat tatttttgat cctttgattg
    24961 gtggcatcat cgataatact cgtacaaaat acggtaaatt caaaccatgg ttagttatcg
    25021 gcggcgttgt tagttccgta ctcttggtag caatctttac caatttcttt ggcttagcta
    25081 ctcataatac gactttattt atcatattat ttgcaatcgt tttcatctta ttggatagtt
    25141 tttattcatt taaggatatc gctttttggt caatgattcc agctttgagt acggaaacaa
    25201 aggaacgtga aaaatttgct acgtttgctc gtttcggctc ttctctaggt gcaaatggta
    25261 ctactctagt cgttgtccct atcgttactt tctttaccta tcttgttact cataaacata
    25321 ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt tcaggcggta
    25381 ctgcgattat cactgccttg ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg
    25441 aaaaagctgg gatcaaagat gtgttcaaag cagttgctca aaatgatcag ttgatgtggc
    25501 ttgcccttgc ttatatcgct tatgcgattg ccaatgcagc aacaactgcc gtattattct
    25561 acttcttcaa atatgtactt ggccaaccaa ctgaattttg gatcgttggt ctagttgctg
    25621 ccttacttgg tttagtagct gttccactat tcccaatgct tacacgcgtc atctctcgtc
    25681 gctacgttta ccttggaggg atcgcaatga tgatttcttc ttactttttc tttacgatcg
    25741 caggttcaag tttgccaatc gttatcatcg ggctagtatt gttctacttc cctcaacaat
    25801 tgattttctt atccgctttg atgacgatca cagactctgt tgaatatggt caatggaaaa
    25861 atggtatccg taacgaagct gttactctat caattcgccc tttactagat aaaatcgctg
    25921 gtgctttgtc taacgggatc gtagggtttg tcgctatcgc ttctgggatg actggtaatg
    25981 ctactgctgc tgatatcact gctcatggcg taacgacttt caaagcttat gcattctatg
    26041 ctcctgctgc tttgatgatt ttctccgcta ttatttttgc ttggaagatt acattgacag
    26101 agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa attggctcca gctgatacaa
    26161 tggaagatga acttcacgat gcagtagatc cttcccatta agaaaggatc ttgaaaatga
    26221 agattacaga aaaagatttt ggccaaggtt atcacttgat aaccttggag aacaaaaaca
    26281 agctagcact ttctatctct gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc
    26341 gagaattagt tttgggattt gacaccgctg aagaatatat cgaaaaagat ccgtatatcg
    26401 gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg ccgcttttct ctcaatggaa
    26461 aaacttacca attagccaca gatccaaaaa ctggacataa tctgcatggt ggtgctcctg
    26521 gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca
    26581 tttttactac tacaagtccc gacggagaac atggttttcc aggtacaatg gatgtggaaa
    26641 tccgctatac tttgacaaaa gataatatct ggcgagtgac ctctcgcggc accagtgacc
    26701 aagatacttt attcaatccg acaaatcatg tttacttcaa cttgacggga gatgcttctc
    26761 agtcaatcga tcaacatgag ctttggctca atagcgaagc ttatgctcct ttgcgaacag
    26821 atagtattcc tataggggta aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac
    26881 ctaaaaaatt agcgtccgtt tttgctagtg atttggatca aaaaaatctt gttgacggaa
    26941 tcgatcatcc atttttctta aaagaaaccg gcttgggtaa agaagccgct cgcttgacta
    27001 gccccgataa aagaatccaa gtagatattg ctacagatgc ttctagtgtg gttattttca
    27061 ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg aaaactggca catcacggtg
    27121 gcatcacatt tgagactcaa acagctccag gtgcagagcg cttctcagca tttggttcga
    27181 ttcatttaaa agccggatca gtatttgaaa cagtaacaga attcaaaatc aaaacaagaa
    27241 aggaataact catcatggaa atcaaaagaa gcttaaacac aaaatttgcc gaaacattcg
    27301 gacctaaaaa aactacacaa tacttctcac caggacgcat caatttgatt ggtgaacaca
    27361 cagattacaa tggaggccat gtcttccccg cttccatcac ttatggaacg caaggtgtag
    27421 cagcacctcg agacgataat aaaatattag tttattccac aaactttgac gatatcggtg
    27481 tcattagttt tactttagat gagctagaat atgataaaaa agcaaactgg gcaaattacg
    27541 taaaaggaat ggtccttaaa ttaaaagaag ctggacactc aatcgatcat gggtttgagt
    27601 tattagtgga aggaacaatc cctaatggtg caggactttc cagtagcgct tcattagaat
    27661 tattagtagg tgttgtatta gaagatctgt ttgatttaaa aacagatcgc ttggaactag
    27721 tccaaacagg taaaaaagta gaaaatgaat tcatcggtgt caattcaggt atcatggatc
    27781 agtttgccat cggcttcggt gagctagatc atgcaatctt attagataca aatacgctga
    27841 aatacgaaat ggttccagtg aaactagatg gttatgcaat cgtcatcatg aatacgaata
    27901 agcggcggga attggcagat tcaaaataca atgaacgccg tagcgaatgc gaagaagcct
    27961 tggcacgttt gcaaaaaact ttgcctatct cttctttagg agacttagat gaagaagaat
    28021 ttgaatcaac tattgaccaa atcggcgatg atacattgat acgcagagct cgccatgccg
    28081 tttctgaaaa tcagcggact ttgaaggcaa aagctgaatt agaagctggg aacttagaag
    28141 catttggtca actgttaaat gattctcacc actctcttcg ctatgattat gaagtaactg
    28201 ggatcgaatt agatacacta gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac
    28261 gaatgacagg agctggattt ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac
    28321 ctgccttcaa aaacaatgtc tatgatgaat acatgaaagt tgtcggctat gctccggaat
    28381 tttatgttgc acacattgga aatgggacaa caaagatcga tccagaagct gttcatgttt
    28441 gataaaaaaa tcaacgatct cagctaaaaa caaatagata aaaataacct ctcagcaatt
    28501 tccaaatgtg agccttttgc tcgtatttga aaattgctgt tttttattga cacggaaaga
    28561 gaacgtgtgt tctaattgtg ttgaagaggt gattgttatg tgggcgaatc aaaatccggt
    28621 atttgtttat gaaaaagaac cttcaagaga tattttatgt attgactgca aatcttttta
    28681 cgcctccgta gagtgcgttg aaaaggatct tgatcccctt acaactaaat tagtagtcat
    28741 gtcctatccg tccgattcga cagaaacacg gggtagcgga ttaatcttgg cttccagtcc
    28801 tgcagcaaaa aaagcttacg gcatcaccaa tatcagccgt gccagagatc ttcccttccc
    28861 ctatccaagt gatttatata ttgttgcccc aaggatggcc tattatatgg aaaagaacag
    28921 agaaatcaat tctatctata aaaaatatgc agacgaagcg aaccatcatg tctattccgt
    28981 cgatgaaagc ttccttgatg taacaaatag catggcactc tttggtaaaa caaatgcgta
    29041 cgacatggct aaactgatcc ataaagatgt gtttgagcag accggaatct acacgactat
    29101 aggaattggc gataacccgc tacttgctaa actagcctta gacaatggct caaaagacgg
    29161 aaagcaaatg tttgctgaat ggcgttatga agatgtacca gaaaagttat ggaaaatctc
    29221 agatatcact gacttttggg gaatcggcaa acgaacagca aaacgtctga aacaaatggg
    29281 tatacattct gtttatgatt tagctcacgc ggattattat cacttgaaaa gcaagctagg
    29341 ggtcatcggt actcagcttt atgcccatgc ttggggcatc gatcgaagtt ttttgggaca
    29401 agaatataca ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata gagattatac
    29461 aagacgagaa gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt
    29521 gagacgatct gcagcaaaaa cacaagtagt cagcttatgg gtaggatact ctttaggcta
    29581 tcttgatcca aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc
    29641 tagcagagat ctagcagatg ctttattgac cattttccac cagcattacc actaccagga
    29701 tattcggcgt gtaggcgtga actgttccga cctcgtatac gcgacaggtt tacagcttaa
    29761 cctttttgaa gatcctgaaa agcagataaa tgaagcgaaa gttgattttg ttgtcgatac
    29821 gattagaaaa aaatacggct ttcaatcaat catccatgcg catagtctct tgcaaggagg
    29881 acgcgccatc gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga gtggattaga
    29941 aggtggaaaa gatgagacgg acagcaaaaa aattcattga ctataacgag tatcgagatc
    30001 ggccatttgg attaaaatgg gggactgctt ttgcgatgga agaactagtc aacggtatca
    30061 aaaaaatgaa gaagaagcac gcaaacagcc tcttcctaaa caacaaatga cattgcaaga
    30121 aatcgatagt atactgacag aagccttttt atctcgtaaa gcagtaatca ttcagctcaa
    30181 tttaaaagat gaatttggaa gactcttgga tgaggtcgaa gggcacttca ttggtgaagc
    30241 ctatgaagat tactttatga ttgaccatca gccgatattg tgggaagacg tacggtatat
    30301 tgagttaaag gaaacaaaaa aatggtttgt gacatgagag gttgtgacag aagtgatcaa
    30361 cttcaaaaaa taagaaagga tttttcgaaa attgcttctt aaatttttgt ggaattttgg
    30421 cttatttttc gaagaagcaa cttctggaac aacgtatatt agatatcaga ggttgtgaaa
    30481 aagtcgctta gttttgagta acaagccaag gcgctgattt cacaacctct gtctatctat
    30541 ccatctattc ggtaagattc atgcatgacg tcttctttat ttagtatgta gagccctttt
    30601 tcttctgacc attcaacata aatcagctgt tttacatctg catagccttc tttcttcaag
    30661 ttctccataa gccatgcttc agtcaattga ttggactgaa gcactttatg ctgaatttgc
    30721 ccttcatcta caagcaggat cgataaagct ttgtcttcat ctgatttttt caatacactc
    30781 atttgaccgg cattctctag gataacatgt ttggcatttt caagaaaaaa gcattcttgc
    30841 tgtcttaaga ttgctcgcaa ctgctccatc tcgatatggt tatttgtcaa ctctgtcatg
    30901 ttgatgacgc cgtctttgat tagaacagat ggctcgcctt tgacccaacg attgaccttt
    30961 tcattttttt ggacgatacg ttcgatacca taaatcatta ctgcccaaag cgcaatggca
    31021 aataggacat gccctacatg gacattgtca tcataggttg attcttctaa gatcccacct
    31081 aaaacaagcg tatagactaa atcaaacggt gtgatttctg acatggattt cttcccaagc
    31141 aaacgtacca ctaataataa accgatcaac ccgaacacca gtttcattgt aattgaaaaa
    31201 atcatttttt ctccccctaa ctatctgtta attaaattat atatgctttc tcacgaacac
    31261 gcatggaata tacaaggaag aaatgtatcc ttttactaaa aaaaggactg tgacactgta
    31321 gtgaccccca aaagttagac ttttttggag tggagaaatc cactcctttt ttttatgctc
    31381 taaaatgata tttttacaca accgttacgg ttgttttacg aaattggacg
//
LOCUS       DAZKOU010000027        31425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000027 DAZKOU010000000
VERSION     DAZKOU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.027"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..560
                     /locus_tag="ACG827_001148"
     CDS             168..560
                     /locus_tag="ACG827_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0483305.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            588..1928
                     /gene="glnA"
                     /locus_tag="ACG827_001149"
     CDS             588..1928
                     /gene="glnA"
                     /locus_tag="ACG827_001149"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0483306.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2654..2899
                     /locus_tag="ACG827_001150"
     CDS             2654..2899
                     /locus_tag="ACG827_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483307.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3121..4731
                     /locus_tag="ACG827_001151"
     CDS             3121..4731
                     /locus_tag="ACG827_001151"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0483308.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4708..4920)
                     /locus_tag="ACG827_001152"
     CDS             complement(4708..4920)
                     /locus_tag="ACG827_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483309.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5023..5892
                     /locus_tag="ACG827_001153"
     CDS             5023..5892
                     /locus_tag="ACG827_001153"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0483310.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6057..7328
                     /locus_tag="ACG827_001154"
     CDS             6057..7328
                     /locus_tag="ACG827_001154"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0483311.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7325..8620
                     /gene="rho"
                     /locus_tag="ACG827_001155"
     CDS             7325..8620
                     /gene="rho"
                     /locus_tag="ACG827_001155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0483312.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8719..8982
                     /locus_tag="ACG827_001156"
     CDS             8719..8982
                     /locus_tag="ACG827_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0483313.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9427..9876
                     /locus_tag="ACG827_001157"
     CDS             9427..9876
                     /locus_tag="ACG827_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0483314.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9933..11351
                     /locus_tag="ACG827_001158"
     CDS             9933..11351
                     /locus_tag="ACG827_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0483315.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11351..12211
                     /locus_tag="ACG827_001159"
     CDS             11351..12211
                     /locus_tag="ACG827_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0483316.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12385..13158
                     /locus_tag="ACG827_001160"
     CDS             12385..13158
                     /locus_tag="ACG827_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0483317.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13305..13670
                     /locus_tag="ACG827_001161"
     CDS             13305..13670
                     /locus_tag="ACG827_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0483318.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13818..15026)
                     /locus_tag="ACG827_001162"
     CDS             complement(13818..15026)
                     /locus_tag="ACG827_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0483319.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15045..15323)
                     /locus_tag="ACG827_001163"
     CDS             complement(15045..15323)
                     /locus_tag="ACG827_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483320.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15516..16172
                     /locus_tag="ACG827_001164"
     CDS             15516..16172
                     /locus_tag="ACG827_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0483321.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16614..17411)
                     /locus_tag="ACG827_001165"
     CDS             complement(16614..17411)
                     /locus_tag="ACG827_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0483322.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17408..18592)
                     /locus_tag="ACG827_001166"
     CDS             complement(17408..18592)
                     /locus_tag="ACG827_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0483323.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18617..19864)
                     /locus_tag="ACG827_001167"
     CDS             complement(18617..19864)
                     /locus_tag="ACG827_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0483324.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20079..21824
                     /gene="dnaX"
                     /locus_tag="ACG827_001168"
     CDS             20079..21824
                     /gene="dnaX"
                     /locus_tag="ACG827_001168"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0483325.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21844..22158
                     /locus_tag="ACG827_001169"
     CDS             21844..22158
                     /locus_tag="ACG827_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0483326.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22183..22779
                     /gene="recR"
                     /locus_tag="ACG827_001170"
     CDS             22183..22779
                     /gene="recR"
                     /locus_tag="ACG827_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0483327.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22843..23487
                     /gene="tmk"
                     /locus_tag="ACG827_001171"
     CDS             22843..23487
                     /gene="tmk"
                     /locus_tag="ACG827_001171"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0483328.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23502..23831
                     /locus_tag="ACG827_001172"
     CDS             23502..23831
                     /locus_tag="ACG827_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0483329.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23845..24783
                     /gene="holB"
                     /locus_tag="ACG827_001173"
     CDS             23845..24783
                     /gene="holB"
                     /locus_tag="ACG827_001173"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0483330.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24819..25643
                     /locus_tag="ACG827_001174"
     CDS             24819..25643
                     /locus_tag="ACG827_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0483331.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25636..25983
                     /locus_tag="ACG827_001175"
     CDS             25636..25983
                     /locus_tag="ACG827_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0483332.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26052..26924
                     /gene="rsmI"
                     /locus_tag="ACG827_001176"
     CDS             26052..26924
                     /gene="rsmI"
                     /locus_tag="ACG827_001176"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0483333.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27033..28154
                     /gene="dinB"
                     /locus_tag="ACG827_001177"
     CDS             27033..28154
                     /gene="dinB"
                     /locus_tag="ACG827_001177"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0483334.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28208..28810)
                     /gene="nrdG"
                     /locus_tag="ACG827_001178"
     CDS             complement(28208..28810)
                     /gene="nrdG"
                     /locus_tag="ACG827_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0483335.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29125..31314)
                     /gene="nrdD"
                     /locus_tag="ACG827_001179"
     CDS             complement(29125..31314)
                     /gene="nrdD"
                     /locus_tag="ACG827_001179"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0483336.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT        10087 a         5633 c         6632 g         9073 t
ORIGIN      
        1 aaggtatctg gtacgaaaat caactagata ccttttttgt gttcttattt ttttgttatt
       61 ttataaaaaa tagagatctc atgttagaaa acctaacata tgcagttgac aatatttttt
      121 ttaattggta cactttagcc aacttataaa acgaaagggg acgtctgatg ggagaaaagg
      181 aattgcgccg atctatgtcc gtatttccaa ttggtacagt tatgaagctg actgatttgt
      241 ctgctcggca aattcgctat tatgaagaac aagatttagt tcatccggaa cgaagcgaag
      301 gaaaccgacg tatgtattcg ctgaatgata ttgatatgtt gcttgagatt aaagactatc
      361 tttcagaggg attgaatatg gctgggatca aacgtgtata cgaaatggca caggcgaaaa
      421 aacaagaagc aaaaaacaag aagccgttga cagatcatga cgttcgacaa atttttcgtg
      481 atgagatttt ggcgcaaggt gggttaagca aaacaggtca ttatcagtcg caaggattgc
      541 aaagacaatt ctttgactga ataaattaaa aaaggaagag ggataccatg gtaaagaaac
      601 agataacagg tgaagaaatc aaacgaatca ttgaggaaga aaacgtacga tttttacgat
      661 tgatgttcac agacatctta ggtacgatca aaaatgtaga agttccagtg agccagatcg
      721 acaaagtatt agaaaataaa atgatgtttg acggttcttc gattgaagga tttgttcgaa
      781 ttgaagaaag cgatatgtat ttgtatccag atctctccac ttggatgatc ttcccgtggg
      841 aaagtgctca tggaaaagtt gctcgtttaa tttgtgatat ctataatccg gatgggacac
      901 catttgccgg cgatcctcgc gggaatttga aacgagcatt gaaagatatg cgcgaccttg
      961 gttttacctc tttcaattta ggacctgaac cagaattctt cttatttaaa ttagacgaag
     1021 acggtgggat cacaacgacc ttgaatgata aaggtggtta ttttgatttt gcaccaacag
     1081 atttaggtga aaattgccgc cgtgacatcg tgttggaatt agaaagtcta ggctttgaag
     1141 tggaagcttc acatcatgaa gtagcgccag gacagcatga aattgacttt aaatatgcag
     1201 atgtggtaga tgcttgtgat aatatccaaa ccttcaagct cgttgtcaaa acgattgcac
     1261 gcaagcatgg acttcacgcg actttcatgc cgaaaccgtt atttgggatc aatggttctg
     1321 gtatgcattg caatatgtct ttgttcaatg atgaaggaaa tgttttctat gataaagacg
     1381 gtgaattgga actaagtgaa acagcttatc atttcttagg tggtttgctg aaacatgctc
     1441 gtgcctacac agccgtttgc aatccaacag tcaattctta taaacgtctc gttccaggtt
     1501 atgaagcgcc tgtttatgtc gcatggagtg gccgtaatcg ttcgccatta gtacgtgtac
     1561 cagaatctcg cggtttatcc acacgtttgg aattgcggtc agttgatcca tctgcaaacc
     1621 catacttggc aatggcagta ttgttgcaag caggactaga cggtatccgt aatcagatca
     1681 caccgccagc agctgttgac cgtaacattt atgtaatgga tgaggaagaa agagaagctg
     1741 cacatatcca ggacttacct tcaactatcc ataatgctat caaagaattg cgtaaggata
     1801 atgtaatgat tgacgcatta ggacaacata tcttctcaaa ttttgttgaa gcaaaacgtc
     1861 tcgaatgggc ggcattcaga caaactgttt ccgagtggga aagagaacaa tatctagaac
     1921 tttattaaaa caggaatacc gtgaattaga ggctgtgaaa tcagcgtttt gacctgagta
     1981 ttagagaaac taacggaaaa atagttcctc atattttgcc gttttttgag ctaataccga
     2041 gggtcagctg attgaacacc gtttagtgga gaggttgtga aaaagctgcc ctgcaacaag
     2101 taataagaat agccaagcaa aaacagcttt tcttgttttt gaattggctg aacttattac
     2161 cgcagatgca agcttttgaa caccgtttac caagaggttt tgaaaaagac gtttagtttc
     2221 gagtattaag aaaaattttg tgaaaatggc tttctacatt tttgcataaa tttggcttaa
     2281 taccgagaaa ctggcttttg aataccgttt atccagaggt tgtgagccta ccatttaact
     2341 ccgaatcaca agaaataatt tcaaaaacag cttctcatgt ttgatggata ttgtgacttg
     2401 tgaccgagga gttggcagga gaacaccgtt tatttagaaa taaggaggca cgacttttgt
     2461 cgcatctcct tattttttac ttatgtgatt cgttaccttc aatgaatccc tacaaggcac
     2521 ttttgttgaa aaaaaggtca tctttgaaaa aattagtgga attgttttgt ttttgatatt
     2581 atttaataga aaagatatgc caaaaatccg agaactgtgg actacgtttt ttacgtaaag
     2641 gaggtaaata agggtgaaag aaaaagattg tttctacgat aagctatata aactgttgct
     2701 gcaagtagga aaaagtagtt ctttgttagg tgggatatta tctatcataa tgtttgtttt
     2761 atttttttat ctattatttc acttaaaatt tttcagtaac gattttctga tttgcttagg
     2821 tttaacgtta gctatgttta tatcaagctt attatgtggg ttaaattttt tttgcactca
     2881 taaaggactt aagaaataat ttctcaaagg atatcgaaaa agaaaaaatt gtaagcggat
     2941 agtattcatt tgagtacttg actttccctt agtcagcttg tattatacac cttgggcacc
     3001 cttttagagg gtcagaagtt tacagaaatt tcggctccct attcttattg aagaataggg
     3061 agctttgttt attgtctgag acttttcctg cacataaatt aaaaaggagt ttttgaactt
     3121 atgacaaaat atatttttgt gaccggcggt gtcgtttcat caatcggaaa agggattgtt
     3181 gcagcttctt taggacgctt attgaaaaac cgcggcttaa aagtaactat ccaaaaattt
     3241 gatccataca tcaacgtgga tccggggacg atgagcccat atcagcacgg agaagtattc
     3301 gtaacagatg atggtgctga aacagatttg gatctgggcc actacgaacg ttttattgac
     3361 atcaatttga acaaatactc caacgtgacg acaggaaaaa tctattctga agttttgcgt
     3421 aaagaacgta agggcgaata cttgggagca actgttcaag ttattcctca tattacaaat
     3481 gagatcaaag aaaaaatcat gcgagcagct aaaatgactg attcagatgt gatcattaca
     3541 gaagtagggg gaacagtagg ggatatagaa tctttgccat tccttgaagc acttcgtcaa
     3601 atgaaagcag atgtcggtac agataatgtt atgtatatcc atacaacatt gattccttac
     3661 ttgaaagctg ctggagaaat gaaaactaaa ccaacgcagc acagtgtaaa agagctacgt
     3721 ggtttaggta tccaaccgaa tattttggtg gtacgtacag agcaacctgt ttctcaaagc
     3781 gtgaaaaaca aattggcaca attttgtgat gtcgaaccag aagcagttat cgagtcgcca
     3841 gatgtagata cactttactc catcccatta gttttacaag cacaaggaat ggatcaaatt
     3901 gtttgcgacc atttaaaact agatacacca gaagcagaca tgacagaatg gaaagaatta
     3961 gaagaacgtg tcttgaactt gaagaaaaaa gtacggattg cgcttgtcgg taaatatgtc
     4021 gagttaccag atgcatatat ctcagtagtt gaagcattga aacattctgg tttcgcctat
     4081 aattcagaca tcgagatcga ttggatcaaa gcacaagaat taacgagaga aaatgtagaa
     4141 gaacgtttga aagacgcaga tggaatcttg gttcctggtg gatttggtga tcgcggagta
     4201 gaagggaaaa tcgaagcgat tcgttatgct cgcgaaaatg acgttccgtt tttaggtatt
     4261 tgtcttggta tgcagatggc ttgtgtcgaa tttgcacgta acgtcgttgg attagaagat
     4321 gcgtcatcag ctgaaacgaa tccagataca gcaaacaata ttattgattt aatggctgat
     4381 caggaaaaca tcgagaattt gggtggaact ttacgccttg gattataccc atgtaaaatc
     4441 aaaaaaggaa cgaagacggc tgaagcatat gatggagccg atgttgtgca agaacgtcac
     4501 cgtcaccgct atgagttcaa taacaaatat cgtcaattgt ttgaagaaca agggctagtc
     4561 ttttcaggtg tctcaccaga taaccgttta gtagaaatcg tagagctgcc agagaaaaaa
     4621 ttctttgtgg gatgccaatt ccacccagaa ttgatttctc gtccaaatcg cccgcaaaaa
     4681 ttaatcaaag catttgtcgg agcatcatta gcagatcacg aaacaaaata atttttgaac
     4741 ttgatagaaa gagaaaagta gcaagagaag gggataagca attttcttgt cacttttctc
     4801 tttttctgat ttgttattat ttgtaggaaa aaagatagaa agcaaaagac ttttttcgat
     4861 taaagcgcta ttggatattt tattttttgc aaaaaacttc ttgaaaattt tcattttcat
     4921 tgattttagt agaaaactac cgaaatgttt ggtaaaatag ggtcgttggt taagaattgt
     4981 cttaacaaat ttagttttac aaaacttagg aggaatttta ttatgccagt agtatcagga
     5041 gctgaatttt taaaagctgc tcgtaaaggc ggttacgctg tcggcggatt caacacaaac
     5101 aacttggaat ggactcaagc aatccttgaa gcggctgaag caaaacaagc accagtactt
     5161 atccaaacat caatgggtgc agctaaatac atgggtggtt acaaaatctg taaagattta
     5221 atcactgatt tagttgaagc aatgaacatc actgttccag ttgctatcca cttagaccat
     5281 ggtgattacg aagctgcttt ggaatgtatc gaattaggct atacttcagt aatgttcgat
     5341 ggttcacatc taccatttga agaaaacttg aaattagcta aagacgttgt tgaaaaagca
     5401 catgcaaaag gtatctcagt tgaatgtgaa gttggttcaa tcggcggaga agaagacgga
     5461 atcatcggta acggcgaatt agctgatatt gaagaatgca aacaaatggt tgctacaggt
     5521 atcgactact tagcttgcgg tatcggtaac atccatggtc aatatccaga aaactggaaa
     5581 ggattagctt ttgaccactt acaagcaatt gctgatgctg taggtccaga catgccttta
     5641 gtattacatg gcggatcagg tattcctcaa gatcaaatcg aaaaagctat ctcaatgggt
     5701 atctcaaaag taaatgttaa cactgaattc caattatcat ttgctgcagc tactcgtaaa
     5761 tatatcgaag ctggtaaaga taaagaaggt aaaggattcg accctcgtaa attattagct
     5821 ccaggtaaag cagcaatcgt tgaagatgct aaatctcata tcgactggtt cggttcagct
     5881 aacaaagcgt aagcatccac ataacagata cgatatatag gaatggagaa cgacttttga
     5941 agtcgctctc cattcttttt tacttaaaac ggcagaccat gctcgtttct tttttttatc
     6001 tatgataaaa tacaagcata tccatgaaaa aagattaata aacaaggtgg aaacagatga
     6061 aaaaaatcgt aatcaaagga aaccgacctt tagctggtga agtaacgatc agcggtgcaa
     6121 aaaatagtgc agtcgcattg attccagctg caatcttagc tgattcccca gtgatcttgg
     6181 aaggtgtgcc ggacatacaa gatgttcatt cattaattga aattttagag atcatgggcg
     6241 caactgtcca tttttccaac aacatactag aaatcgatcc tcgagaagtc gtatcgattc
     6301 cgatgccgca cggaaaaatc aatagtttgc gtgcttctta ttattttatg ggaacactgc
     6361 ttggtaaatt tggcgaagcg gtagtaggcc tgccaggtgg ctgttattta ggccctagac
     6421 cgattgacct acacgtcaaa ggatttgaag ctctaggtgc tgaagtgaca aatgaacatg
     6481 gtgccatgta tttacggaca gaaaataaag gattgcgtgg caatcggatt ttcatggacg
     6541 tggtttcagt cggagcaacg atcaatgtga tgttagctgc tgtaaaagca aaaggacaga
     6601 cagtaattga aaacgctgca cgagaaccgg aaatcataga tgtggcaact ttactgaata
     6661 atatgggggc aaaagttcga ggagcgggta cagatgtgat ccgtatcgaa ggagtcgaaa
     6721 cattacatgg ctgtcggcat ttcatgattc ctgaccgcat cgaagcagga acctatctag
     6781 cattagcagc tgcagtagga aatggtatca aagtgaaaaa tgtgatcttt gaacatttgg
     6841 aaagctttat agcaaaattg caggaaattg gtgtccatat gaaaatcagc gaagatgaaa
     6901 tcgaagttta cccctcgaaa gaattaaaac ccgcaaacat catgacctat ccttatccag
     6961 gatttgccac agatctgcag cagccactta ctgcgttact attgatgact acaggcactt
     7021 cagaaatcat cgatacgatc tatgcgaaac gtgtgaacca tgtgccggaa ctagcacgaa
     7081 tgggcgctga tattaccgtg gaaggaaata tgatcatcgt gaatggtcca aataaacttc
     7141 acggcacaga agtcgtggct tctgatcttc gggcaggtgc ttgtttagtg attgctggac
     7201 tattggcaga agggacaacg acgatttaca atgtagatta tatcttacgc ggttatgatc
     7261 atattatcga gaaactaaca gccttagggg catcaattga aatgatagag gaagtgggag
     7321 ctgaatgagc gattatttga caatggcaga actagagaac aaaacgttaa aagaagttta
     7381 tagttatgcc aaagaattta aaattcctta ttacagtaag atgaataaaa aggaattatc
     7441 tctagctgtg attcgtgcac aagcagagaa acaaggattt tattatatgg aaggaatatt
     7501 ggatatcgtt ggtcaagatg gttacggttt tttacggcca atcaattatg gaccaagtgc
     7561 agaagatatc tatatttctg cctcacaaat cagacgattc agtttgagaa atggggacaa
     7621 agtggcagga aaagcccgtc cacctaaaga atcggaacgt tattatggtt tgatgcacgt
     7681 ggaaagcgtt aatggaaaag atccggaaga agcaaaagaa cgtccgcatt ttccagcctt
     7741 aacacctctt tatccgcaaa gacagcttca actggaaaca acaccgcaaa gactatctac
     7801 ccgaatgatc gatatctttt ctccaatcgg ttttggtcag cgtggattga tcgtcgcacc
     7861 gccaaaagca gggaaaactt ctgtattaaa agaaatcgca aatggtatca ccgaaaacta
     7921 tccggaagta gaattaatcg ttctactgat tgatgaacga ccggaagaag taacggatct
     7981 agaacgaagc gtgaaaggcg acgtggtatc ttctacattt gatctgcagc cgcaaaacca
     8041 tactcgagtt tctgaattag tattggaacg cgcaatgcgt ttagtcgaag ataaacgaga
     8101 tgtcgttatc ttgatggata gtattactag acttgctcgg gcctataact tagtcgttcc
     8161 accaagcgga cgtacattga gtggcgggat tgacccagcc gcattgtata aacctaagaa
     8221 atttttcggg gcagcaagaa acatcgaaga aggtggcagt ttgacgattt tagcgaccgc
     8281 tttagtcgat acaggaagcc gtatggatga cgtgatctat gaagaattca aaggaactgg
     8341 aaatctagaa ttacaacttt cacgtgaatt atctgaacgt cggatcttcc cagccatcga
     8401 tatcaaaaaa tcaggtaccc gaaaagaaga attattgatg ccaagtgaag aattagaaga
     8461 aatttggaaa cttcgaaaaa atatgactgg agattcatta gaatatacgg aacaactgat
     8521 ccgctttttg cgtaaaacaa aaaacaatca gcaatttttc aaagaatttc aagatgtctc
     8581 ctttggtaaa aaaaatccga ccagaaatct gaaaagataa ttgcaacctt gactgaaaca
     8641 tgttaagatg ttaatgttgt aaatcgacaa atcaactctg gttcagcaaa tggagcaggg
     8701 caaaggagcg aaaaaatcat gaaagaaaac atccatccag attaccaccc agtagtattc
     8761 atggattcaa caactggttt caaattcttg tcaggttcaa caaaaacttc acaagaaaca
     8821 gttgaatggg aagacggtaa tacataccca gtcatccgtg tcgaagtaac ttctgactca
     8881 catccattct acactggacg tcaaaaattc acacaagcag acggacgtgt ggaccgtttc
     8941 aacaaaaaat acggtctcaa agacgcaaac gctgcggaat aataaaaacg ctgataactc
     9001 aacgtttagc agactatctg atagggatag tctgtttttt tatgtttttt gccaatttaa
     9061 aacttcgttt gatgctgaaa aagtcgtagg atcataatgc acaaaacaat gaaaaactag
     9121 tatatagttt taattatcca cttgataaaa acaccttata ttaacgaaat gaagaggggt
     9181 tatttcgaag taaaaaacca tttacctaaa attagaagct aagctttcct ttcaaggaag
     9241 gctttttgtt ttgttataga cttgcgattg tttgtgagct agatcgttca ttttaatgac
     9301 ttatacacgc ggatgatggg tctttttcct ctgttcattc ataatattga acggtacagc
     9361 tagcgatgct atactgacct cagttccaag aaaacgttaa cattttttgt gggggagagg
     9421 ttttttatga ggtttgatca attgatcgca gaagaactga ttgttttcga tccatcgata
     9481 gattcaaaag gatcattatt tgagcgaata ggggatgtgt tgaaaaaaac aaataaggta
     9541 agaaatgttc aaaaagtcgt taaggcacta ttcaaacgag aggaggagat ctccacagga
     9601 atagaagatg gatttgggat tccccatgca aaaagcaaat atgtgacaaa accagcactg
     9661 tgtttttttc atacagccaa attaacggac tatagcggga tagatgaaat gccaattgaa
     9721 tatatttttg ccatcgtagt tcctgaaaaa tcagcagata tgcatctaga gattctcagc
     9781 acactctcca gacgattgat gaatgcagat tttagaaagg gaataaaaga agccactacg
     9841 ccagcagata tcatcagaca gattagtcaa gaatgaaaat agtagagtct ttgtccgagt
     9901 atcgatagtg aagaaaaagg aggaaaataa tcatgaaaat cgttggtgta actgcttgcc
     9961 cgactggaat tgctcatact tatatgtcgg cagaaaaatt agaagtaaca gcaagaaatc
    10021 taaaccatca agcaaaattc gaaacacaag gtgtaaaaac agaaaatcag ttatctgaac
    10081 aagaaatcaa agaggcagat gcaatcattc ttgcagtaga taaagaaatc gaattggacc
    10141 gatttgccgg gaaaaaagta aagcgtgtct ctacaagccg agcaatcaaa gaaccgcaag
    10201 tagtaataga cgaagcattg agagatattg gcgtattcgt tgtgtctaat gaaaaagaac
    10261 cagctgcaaa tgagaaaaag ccgacaatct ataatcattt catgaatggt gtgaattaca
    10321 tgcttccatt tgtgatcgca ggcggaatca tcatcgcact gagttttgcc tttgggatta
    10381 cagcggctga tcccaactca gcagactata atgtgttagc agctgcattt tcaaggatcg
    10441 gaggagatac agcgtttgcc atgatggttc ctgcgttagg cgcggggatt gctacatcga
    10501 ttgctggaaa agcaggtttt gccccaggaa ttgttgccgg attactggcc tctacaggtg
    10561 gttcaggttt tcttggcgga atgattggtg gcgtgctagc agggtatatc tgtgattttc
    10621 tggcaaataa aattagcgta aaaaaagaat tttctgctat ttatcagctg attgtcgtcc
    10681 cctttttatc gatcttaatt attgggcttt tgatggtatt tgtgattgaa caaccgatca
    10741 catgggtgtt ggatgctttg acaaattggc tgaatagctt gggaaataca tccgggctat
    10801 tgttcggatt acttatcggg atcatgatgg cagctgatat gggtgggccg atcaataaat
    10861 cgacttcgac tttctcgatc ggtttgatgt cagctggagt aaatgcaccg attgctgctt
    10921 gtatggctgc aggaatgaca cctcccctag gtattgcttt agccacttta ttattcaaac
    10981 ataaatttac caaagaagaa cggacttcag gtcaatcatg ctggatttta ggcgcttcgt
    11041 atattacaga aggtgctata ccgtttgcgg tatctgaccc gatccgcgtg atacctagtt
    11101 tgatgacagg ttcagcagtc gctgcaggga tttccatggc agtaggagtt acttcaatgg
    11161 cacctcatgg agggatttgg gtgatgctaa ttccaaatgt tatgaacaat ttacctatgt
    11221 atgtcttggc aattgcatca ggtacagtca ctacagcctt tatgcttgga ctattgaaaa
    11281 aaacagtgaa agagaaaaaa caagacaagt ggacgaaaaa tgaaaatcta acaaatgagg
    11341 tggaagcgta atgttatata caatgaaaga gttattgaaa gtggctaaag agaatcaatt
    11401 tgcagtacca gccttcaata tttgcagcta tgatatgatg aaagcaatca tcgaagaagc
    11461 agagagatta gaagcaccag taatcttaga aattcatcct gatgaaatcg cctatttaag
    11521 tgatgagttt gttgcggcag taagagccta tgcttataca agtaaactgc cgatcgttat
    11581 ccacttggat catggcggta cgatagaaga tgtcttacgg gcgattcgaa acggctattc
    11641 ttccgtgatg atcgatgcgt ctttgaaatc attcgacgac aatgtacaga ttacgaaaaa
    11701 agtggttgcg ttagctcatg cggtaaatgt ctcagtggag gctgagcttg gaacgattgg
    11761 gaataatggt tctgctgaag gaggtgctga aacaatcatt tatacagatc ctgatcaagc
    11821 gaagattttt gtgaaagaaa caggaatcga tacacttgct gtagcaattg gaacagcgca
    11881 tggcttgtat ccgaaaaaca gaactccaaa attaaatatc gacttactga aagaattgaa
    11941 ccaacaaatc gatcttccat ttgttttaca tggcggttca ggtaatccgg acgcagaagt
    12001 cagcgaagca gtaaaatacg gagttgccaa agtgaatttg agctcagatc ttaaaagcgt
    12061 ctttttcaat gctttgagag aaatattgag tgaaaatcca tcgatgtacg agccgaatgc
    12121 aatttatcca gcagctaata aaaaggtaca agaagtggtc aaacataaaa tggaaatttt
    12181 gaacacagta ggcaaggcta gtttatacta aaaataaaaa tgtgtggtca agagggtatg
    12241 acaatagtct tgtcataccc tcttaaccac acacgatgtt cagaagtagt tttttcgaaa
    12301 aatttcttct tatttctcga agccaatcac ttctgtcaca acctcttttt tcctgttact
    12361 atataaagta aatggggtga ttttatgttg aatgaagtgg agaatagaca gcaggagatt
    12421 gtgaattatc taaaaaacaa ccaatttgca agagtcaccg atttgatcga gttagtaaat
    12481 tatagcgaag ccacgattaa acgtgacctt gtttttctgg aaaaaagtgg attgatccgt
    12541 agagttcgcg gtggcgccat gcttattgac aatcgaaaaa ttgacgtgcc ttacctgatg
    12601 aagatcacca aattagaaga agaaacagaa aaacgtaaga ttgcagatac agcttcttct
    12661 ttgattcgag atgatatggt cttattcttg gattccagta caacctcttt acacttagtc
    12721 agaaacctag caagatttga tggtttgcag atcattacga atggcatcgt cacagctgct
    12781 atgttgagtg aatatacaac ggcaaaagta tctgtattgg gaggggcaat tgttccgaaa
    12841 cgagcaacaa tcaacggttc aaaagcctat aacgatgcat tgtcatatta tgcggattta
    12901 gcgataatga gttgtcgcgg gctagatttc agccaaggag taacagaaac tcatgaagga
    12961 gaggcaatgg tcaaaaaagc ttttagaaaa caatcagacc ggcttgccgt tttagttaca
    13021 cagcaaaaaa ttgcacataa attcatgcat caagccattg cttttcatga aatcgattat
    13081 ctggtgacaa gtaaagaact gacaaaagaa cagtatgcga aagtaaagga acaccatatc
    13141 gaatgtctgt acggataaat tattacgtga atgttctaag tggcttcctc atttttagat
    13201 aggaccttat ctttgctcgc ttctctcagc cgatggtata ataatgaaaa ggttatcttg
    13261 ttagataatc ttactttttt atccaggaga aaggagtgca gaaaatgaaa catgtgaagg
    13321 cgttggtagt gaaggcaatc atgatctggg cgattctgtg ggtcgtgctg acaggtttat
    13381 acggtgttag ctttatggat tcaacgattg ttggggtaat tattgtagtc atgatctatg
    13441 tgctagggga tctgttgatc ttacgtaaag tagggaacat cgcagcgacg atcgcagatg
    13501 ctggttcagc tgcagttatc ttgtggctct atctatattt tatgaatgat acagtcgata
    13561 tttggatgaa agtattgatt cctgctctat tgatcggtgt ggcagaatgg ttcttccaca
    13621 agtggttgct aagtcaaggc atcgtaccag atgaacggaa aatggaataa atcaacagtt
    13681 aaactattct tatcgataaa tgaaagcttt aaaaataaat agatgtttaa ttaagaataa
    13741 tcataaacta tttttactca aaaaaccgct cagattattt tcagatctga acggtttttt
    13801 ttatctattc atcaagatca aatttcattt ttcaatgatt caatcaattc gccctctgct
    13861 tcatgactat gttttcggcc attaacaaga tagagtgctg tacatatcaa tggaatgact
    13921 ccggcaatca catacaatcc tctaaatgaa gtaagagatt ttagcatccc catcaaataa
    13981 ggaccaacgc ctaagcctag gtctaaacca ataaagtagg tagataaagc aatactgatt
    14041 cgatgttcgc ggacgatttt taaacaaaca gcctgtccat ttgacataaa cgtaccataa
    14101 cctaggccga ctaatgctcc agagataagc aatgtcacac tattcgtcgt tacactcagt
    14161 aaaaataatc ctgctgttaa gaataaatag ctaggataca tcacgtattg ttctcctttg
    14221 acatcgaaaa tccttccagt caatggacgt gtaaatgtga tgaccaaagc atatacgacg
    14281 aagaaaaatg acccagcagc aactaagtgg attacttttg tataagaaga taaaaatgat
    14341 aaaacacttg aataagaaag acccatcaaa aaggcaattc ctgtaatgaa aagtgctttc
    14401 ttttcaataa aactattgat attccatgag cgaagagata ttttttgttc ttcagataat
    14461 ggaatatttt ttacaggaaa gacaaggcag gcaatcgtaa caaagaagac cagtacaatt
    14521 gaaaaccaga taatgaaacg aaaatcagtc atattcagca aaatcattcc taaaaatggc
    14581 ccaacagcgg cagctaaact agtacttaat ccataataat tgatgccttc ccctttttta
    14641 gatgcaggga tatatgcagt aacgatagca tttgtagctg tagaaactgt accgtagccg
    14701 aatccattca agaatcgaac taaatacaat gccccgatag ttggcgtata aagatatgct
    14761 aatgttgaaa ggagatagaa gatcgcgccg cctcttaagg taaacttccg accaaatagt
    14821 tctaactgtt tccccatgaa taagcgtgcc agcaatgttc cgatgatata gatcccagaa
    14881 gcaaaacctg cttggctcaa gctggcgtgt aaggtgtctt gcgcaatgac tgccatgatg
    14941 accatcaaca gataataaat caggtagaca ataaaattga tcaatgtgat tccaataaaa
    15001 tatttattaa ataatttctt ttccatgtat gttatacatt ccccttaagc tgaatatgct
    15061 tttaattttt gtatttgaac ttctacatcg tgataattgg atggatagcg atcaacaagt
    15121 tggcgaataa ataaatctct tgtagacata aacagattta aaaattcttc gcattcgttt
    15181 gttggattct catccactgc tttgatcaat tcgatcgagt tttgatacgc atcatttaaa
    15241 aagactaaag aagctttcat atcaaatctt gtttgatatg ctgtttctaa tcgattaatg
    15301 attgtgttca tccagtaatt catgtttttt ccctctattc ttaaaaaatt ttacttctgc
    15361 agtataacga ataaaaaaaa ggaaaaaaat cgcataagtg atggaaaaag gtacttaaca
    15421 aatgttaata tcattagtaa aatacctttg gactttaaaa agttttaaag tactgtatga
    15481 tctttttaga tgaaagtttg gatgggggag catcattgga tagtagagtc ttaaaggaat
    15541 atttggataa aaatgatttt ccgattatac gcaaaggata tcataaatat ttgacattcg
    15601 aagggatgga agattcccat acgtatatct tgaaagaggg aacggtaaaa gcaagtgtta
    15661 tttcaaatga tgggagagaa tttaaccttc gctatatcaa agatcttgaa atcgtctctc
    15721 ttttaaaaga cgaatattca caattcatcg attcgccgta taatattcga gttgaatcag
    15781 aatatgcaga gttttatcga atcaatcgtg tgcgcttttg gcaaaatatc aaccaagacc
    15841 caaagctaca acactatgta aaagagtatt accgctatcg tctgctttat tcgatgaaaa
    15901 aaatgcagca aatgctatta aacggaaagc tgggagctgt ttgtacgcaa ctttacgaat
    15961 tatatgattt attcggtgtg cggacagaag aagggatgct gatcgatttt tcgatcacga
    16021 atgaagaaat cgccaaattt tgtggcatct caactgctag cagtgtaaac agaattttaa
    16081 aacaactgaa aaaagatggt attatcaccg ttaaagacca tcatatcgtt attttgaata
    16141 tggacttgtt agaagacaat attatctttt gatgaacaaa gaggttgtga aatcagcgtt
    16201 ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg ccgttttttg
    16261 agctaatacc gagggtcagc tgattgaaca ccgtttacca agaggttgtg agcctgccgt
    16321 ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg
    16381 tgacttgtga ccgaggagtt ggtaggagaa caccgtttac caagaggttg tgaaaaagct
    16441 gttctgcatc aagtaataag aagagccaag taaaaacagt ctcttatgtt tttaacttgg
    16501 ctggacttat caccgtagat gtaagctttt gaacgccgtt tatttgcaac aaaaggggct
    16561 gtgacacgat ttttgtcaca gccctttttt tgcgaacatt catttagcaa tttttagaaa
    16621 cttctgccgc caccgccaaa agtcccgcca ccgctagaat gggtagtact tccgccaccg
    16681 ccgcttccag gaggtgggct tttggggatt cttcttgtcg tgacaaaaga atttgtcaga
    16741 cgatcggttt tttcagttaa tttgatactg gatttttctc ggaatggata tttgtagatg
    16801 cctgatttca attgataacg cgaaacagtc acaaaataaa agacaagact aaggataaga
    16861 gccaaaacaa cagcaatgat gatttctact gtcgtcaaaa ccttatagcg tgtaattttg
    16921 ccagtttctt cgtctacacg ataatgtcct cctggaactc cgtcttccac atatgttgct
    16981 gatttagaca ggtaagcttt tgcagcattg taataggcag catttgtcat ctgatcataa
    17041 acatcatcta agatcgaatc gattctgcta tcagtcaaat aatcgatcat attaccagaa
    17101 gtcgaaatgt agatttcacg ctgtcccata tctaatagca atactgagcc attttgatca
    17161 tttccgactg cgtctcttaa gtaattatca gcaaagtctc ttggttcttc tgaattagat
    17221 gtagtcgtta cgatcaagac tcgtcctttg attttttcat tcagcttttc tgcctgttgt
    17281 tccaaagatt ggatttgatc tgttgtgaat aacccagcct catcatttac cgtagctgta
    17341 tctgcatgag tagccaccgg ccagccaaca cttactagca aaagaaagaa taaaaaaaat
    17401 tttttcatca tatcaagtaa cctccgatca gaaagaggat cgctagccca gcgaaaatcc
    17461 caagagcggt cagtcccaat tttttctggc tgatcggcag gacaccgctg acctttcctg
    17521 tttgtccatt cattgcgtag tagtagactt ttttacttga gtcattgctt cgataagtaa
    17581 caagccatac cggcagtaat acgtaattat ttacttcgtt tgtgattgaa gcagaagttc
    17641 gcacaccggt caatgttgta tatccatttg cggtatcttt taacagcttc tctgaatagt
    17701 cttttaattc agattgaatc tgtgatttga ttgcctcata ttcgatatct cttttttctg
    17761 cttggaaccc ggctaaatac tggcttttaa aatcaattgc tttatctaac gggaaaggtt
    17821 gcacgccttc gaccatcttt tgttgcgtat tttttgataa ggcatttttt actagttctt
    17881 taaaagaaag cttgcctgct cttgaaacag caaattgctt cgtttctgtg tattcgatgt
    17941 cacctatccg ccatatgcgg ataactgtac cattcgcctg catggcacca tctacttcag
    18001 catctgtagc ccaataaggg aaatacacac ctgtcaactt atcgatttga tccttattga
    18061 aaaaagcttt gggaacaaac cattttttct tggtccaagc gagaaatctg ttgattgctt
    18121 cttctttctc gacagcaaaa ggaagtactt tatttggcaa gaattctccg cttaaacgtc
    18181 ccgatagtac gacgggatta tggcagtagt aacaataagt cgcagcagtt gtcgcatcag
    18241 tcacgatctc tgcaccacag ttgggacaaa gaaaaagatc tatcgctgcg gtattatctg
    18301 cagattcgtc cgctgtatct gaaaaaccgc ctgcctcgtc aaccgctttg cgctcggctt
    18361 cattcatatc ggcaagctgt tcttgggctg taaaagtaaa atcttcggta ggtgaaggtt
    18421 cttctttgct ttcttcgcca gccatacgcg cttctttttg cttttgctca tattggctga
    18481 cttcttcttc tgtatagata ttcaggcagt attcgcaatg aaatttttgg tctttgggat
    18541 caaaagtcaa cggaccgccg caattaggac atttatgtgt aagaacatcc atcagctgca
    18601 cacctttcta aataaattaa ttaacgggga ttcctttaaa cggtttacca cagtttggac
    18661 agaatttagg gatgccgttg gaaagatcca cgatctcatt gcattcactg catcgcattt
    18721 gtagcttagg ctgaacgttt tctacaggtt tggcagctcc gcagttggaa cagaattttc
    18781 cggaattttc tgtcccgcaa actggacatg tccaagtatc cgcatttcct tgtgcagttt
    18841 gttgattttg tttttctgct tgttgtttga tttgttcttg gttattttga gctgcttgag
    18901 aaagataaga accatttgca ttcattccca tacctacacc cataaagccg gtcattgctc
    18961 ctgcttcatt ttttccggct gcttccatac cacgtgcaat ggaaccttgc acgtagcctt
    19021 cacgtacgct aggatcacct agcatcgccc cttcgttacg catattgatc agtttcacag
    19081 aatcatcggt gtaagaaatg ctggctacag ccacagaaac aatctccatc ccacgtaatt
    19141 cacgccaaga atcatccaat gcagtatcca tgtatttgct taattccaaa ctcttagatg
    19201 gtacataaga aatccgttga ccatctgcag acatctgatt gattgctgat tgtagcgctg
    19261 ttaggaattc agctaaatac tgctcattga tatcattgat ttccacttgt gttttgtttt
    19321 tagggattgc gtttgtataa aataaaatcg ggtcaacgac atgaatagaa taagtgccat
    19381 gtgcccgtaa gaataattct gcgttataga aattgtcaaa gtagttcaac ggcgcgcttg
    19441 taccgaattt gattcccttt atttcttgga gattgatata aaaaacttgt tgtttctgcg
    19501 gtgtgacgcc gccaaatttg aatcggtcaa aagtttcagc aattgcttct ttcagtgttc
    19561 cattgaacat ggaaggagct gcgtcatttt taatggtgta ataaccttcc tcagcggtat
    19621 aatcgatgat ttttccacca tcgaccaaaa gcatcatcat gtttggatag acatggacga
    19681 ctgttccgtc tgttaaaaca tcttctgttc ctttacgatt ggagccacgt ttgtcatctt
    19741 tgcggacttt gacacctttt gtcatgaccg ttgtatcact catattgtct ggttcgatga
    19801 cttctagcca ttgatctgcc agaccgccgc caaccatact tgttgctgct ttgataagac
    19861 ccataaaaaa ttcctcctct gattcagcta gtgccaaaca attattgttt atttattata
    19921 ccataggaag atgtgaaact tctcggacta gagacggaat tgaaatggaa aattgcgtat
    19981 tgtttaaata cctgaaaaag ccgagaagga tagtagtttt tttacctaat atcacgtata
    20041 ataagagata gtgaattttt taaaaaaggg ggatccttat ggcatatcaa gcactttatc
    20101 gagtctggcg ttcgcagcgt ttcgaagaca tcgttggaca aaaagctgtg acacagacac
    20161 taaaaaatgc tattgtttcc cacaaaacct cacatgccta cttgtttaca ggacccagag
    20221 gaaccggaaa gacgagtgca gcaaagattt tcgcaaaagc aatcaactgt ccgaacagta
    20281 aagacggtga gccatgcaac gaatgcgaaa tgtgtcgatc aattactgct ggtacgcaag
    20341 aagatgtgat cgaaatcgat gctgccagca ataacggagt cgaagaaatc cgatttatcc
    20401 gtgatcgggc aaattatgcg cctacaaagg cagcgtacaa agtgtacatt attgatgagg
    20461 tacatatgct ttcaacagga gctttcaatg ccttgttgaa aacattggaa gagccaaaag
    20521 aaagtgtcat attcatctta gcaacgaccg aacctcacaa aataccagct acgatcattt
    20581 cacggactca gcgatttgat tttaaacgga tcaatacggc agatattgtc gaacatcttg
    20641 aatttatctt gaaagaatcg actatcagct atgaaccagc tgcattgaat gtgattgccc
    20701 gttctgcaga aggcgggatg cgggatgcat tgagtattct tgatcaggca atctcgttta
    20761 gtgaaggaac aattacttta gatgatgcca tgcaagtgac aggtagctta actgacgaaa
    20821 tgatggacag ttacttgagt gcttgcgtga aaaaagaagt accagaagca ttggaaacat
    20881 tgaaccaaat tctcgcagcc ggcaaagaat cacgtcgctt tctagaggat atgttacaat
    20941 actgccgtga cttgctgatg tatcagcaag cacctaacct agttgaggaa caatcgagta
    21001 cgatcagaag tacatttaca gaattggcac aaactgtttc agcggatcag ctgttttcaa
    21061 tgataaagat tttgagcgat actcaaaatg aaattcgttt taccaatagt gccgctattt
    21121 atttggaagt cgctacagtc aaacttgcac agctaccgtc tgcttctgtt ccttcagcag
    21181 atgtagcatt tgcagatgat cagatagtaa aacagctaca acaagagatt aaggaactaa
    21241 gaaacgaaat caaggaacta cgcagtaata caggaattga gaatacaaaa gtacagccgg
    21301 tacagcaaac aaatcataaa aaacagacaa gtacctatcg tgtaccgacg gaacgagtat
    21361 ataaagttct gcgggaagcg acaagacagc atctgaacca agtaaaagaa gtatgggaag
    21421 acttgctgat gagtctatct gtcactcagc gagcaatgct taaagcaagc gaaccagttg
    21481 cagctggacc tacaggacta gtgattgcgt ttgattatga gattgtttgt caaagagcag
    21541 caaatgacga agaattgcag ctttcggtac acaacaactt aagccgaatg atcaaagatt
    21601 atgcgcctga tcctgtttat attacaagag aaagctggcc agaacttcga agaaattatt
    21661 tgacgcaggc acaagaagag ggaacagaaa actcttttgc ttctgaagac tcagaaggag
    21721 aaatcgagtt gcttcctcaa gaacaagaag taccagaaga aacacttgta gtaaaagcag
    21781 aggaactgtt tggctccgaa gctgttacag tggtagatga ttaataaaaa ggagtagata
    21841 attatgatgc gtggaatggg caatatgcaa ggcatgatga aacaagtaca aaaaatgcaa
    21901 aaagaaatgg cagaagcaca agagctgcta aataaaacag agtttacagg taaagccacc
    21961 aatgaattgg tcaaagcggt ttttacaggc gatcgtcgca tgaaagatat ccaaatcaat
    22021 gaagaagtcg ttgatcctga tgatatcgag atgcttcagg atcttgtcgt aatggcagtc
    22081 aatgatgcga tcgcacaaat tgatgtggaa actgagaaaa aattagggaa atacgcaaaa
    22141 ggtttgccag gattttagtt ttaaactgaa ggagtaatga atatgcaata tccagaacca
    22201 attgcgaaac tgatcgacag ctacatgaaa cttccaggaa tcggtcagaa aacagcgaca
    22261 agactcgcct tttatacgat cgatatgaaa gatgaagtag taaatgaatt tgctaaatct
    22321 ttgttaagtg taaaacgcga tttgcatttt tgtagtatct gcggaaacat cacagaagaa
    22381 gatccctgcg aaatatgtag aaatccttca cgtgatcgaa gcgttatcct agtggtcgaa
    22441 gagccgaaag atgtaatggc aatggaaaaa atgcgagaat atcacggact ttatcatgtt
    22501 ctccatggtg tgttatcacc gatggaaggg acaggtccag aagacatcaa tatttcttct
    22561 ctgttgcagc ggctgcatga tgcagatgta aaagaagtga ttatcgcaac aaatgcaaca
    22621 acagagggag aagcaacagc tatgtatctc tcacgtctga tcaaacccgc aggtatcaaa
    22681 gtgactcgtc tagcacatgg tctgtctgta ggctctgata tcgagtatgc agatgaagtt
    22741 actttattga aagctgtcga aggaagacgt gagttataaa tttctaatta agtgtttttt
    22801 cagatacaat ataaaagcag cacaagagat gggggcaaca atgtgaacgg actatttatt
    22861 acaatcgagg ggcctgatgg tgcaggaaaa accagcatct tgaatgaact ttaccctctt
    22921 ttgaaagaag tagcaaaaac agaaatcatt caaacaagag aaccaggagg cattccgatt
    22981 gctgaagaga tccgagcagt gatccttgat ccaaaaaatg atcggatgga tgaacgtacg
    23041 gaagcactgc tttatgcagc agcaagaaga caacatctag tcgaaaaggt tctgcctgct
    23101 ctggcaaaag ggaaaatcgt tttatgcgat agatttgtcg acagttctct tgcttatcaa
    23161 ggtgcaggcc gacgcatcgg cgttaccgag attgctcggt tgaatgagtt tgctacagaa
    23221 ggtactacgc cggattttac cctttatctt gacgtagatt cagacaccgg attaagaaga
    23281 atcaagaaaa acagacaaaa tcaaatcgac cgtttagatt cggaaggttt ggaattccac
    23341 cagcgtgtac gtcatgctta cttaaagctg gcagaagaga atccggaaag aatccacaaa
    23401 gttgatgcaa gaaaaagctt tgaagaagtg ctgcaaacaa gttatcatac aattattgaa
    23461 cagtatccgc aattttttga aaactaggaa ggtgaacgtt tatgaaaatt attttagcga
    23521 ttatccaaga caaagacagc aaccgtttat caaatgaatt gattgatgca aatatccgtg
    23581 ccacaaaact atcatcgaca ggcggcttcc ttaaggctgg aaacagtacg tttatcgtgg
    23641 gtattgaaga tgaccgtgta gaagaagcat tggaaatcat caaaaaaacg tgtgaatcaa
    23701 gaaagcaatt tgtatctaca cctgttacac tggatatttc gatggatggg ggcgtccctt
    23761 atccagtaga agtagaagta ggcggtgcca cagtcttcgt cttacctgtt gaaggattcc
    23821 atcaatttta ggaggagctg agctatgaat caagaaataa acttggctca aatgcaaccg
    23881 atcgtgtatc agcagcttca acggagtttt gagcatgggc gtatcgctca tgcttatctt
    23941 tttgaagggg agaaaggaac aggaaaacat gaagtaggta tatggcttgc acagcacctg
    24001 ttctgtaccc aaatgaaaga caatctgcct tgtggaaaat gcaacaattg tcagcggatc
    24061 gaaaagaaag aacatccaga tgttttggtt attgaaccgg aaggacaaac catcaaagtc
    24121 gatcagatca gacgtttgca gacggaattc agtcgcagcg gttacgaatc aagaaaaaaa
    24181 gtttttttga tcaaagaagc tgaaaaaatg aatagcagtg cggcaaacag tttgttgaaa
    24241 ttcttggaag agccccctgg agatttttta gcggtgttag aaacagatgc agtaggaaga
    24301 attctcccta ctattcaatc tcgttgtcaa gttctgcatt ttcaagaact ttcgaaagaa
    24361 gcactgatcc atcgattgca agaagagcag attccattgg aaaaagcaaa acttttggct
    24421 ttcttaacaa acagtttagg aaaagcagtt gaaatctctc aagatgaatg gtttaatgat
    24481 gctaaggact tgatttatca atggttcgtt tacttgcaaa agcaagatac tcaagccttt
    24541 atctatgtcc aaaaaaaatt ggtaaaatca tttaaagaaa aaacacaaca gtttacagga
    24601 ttatctgtct tgatgtttta ttatcaggaa gcacagcgtc aagcagtagc aaacggcgaa
    24661 tatggcaaag tgaaacagat aaaccagata attgaacgga tattgatcgc agagcagaaa
    24721 ctacgcagca atgtcagttt tcaggctgtt gcggaacagt tcgttttgca tacgattttt
    24781 tagcttgccg caaaggatga tgatcaagga ggatggaaat ggtagaagta gtaggtgtcc
    24841 gatatagaga tgcaggtcat atctattatt ttgcaccagg agactctgaa tatgtttata
    24901 gtgacaaggt attagtcgaa tctcagcaat ccttacatat tgctactgtt gtccttccta
    24961 aaaaagagat tgatcccaat gaccttcctg aagctatccg tccgatcttg cataaagcgt
    25021 cacaagctga attagaaaaa gacaaaaaaa acaaaaaaga tgcttatgat gcattgaaaa
    25081 tcgcaaaaga aaaagtgcag ttgcgtgagt tggaaatgaa aatggtcaaa gccgaataca
    25141 catttgatcg aaataaattg attttttctt tcacagcgga cgggcgaatt gactttagag
    25201 aattggtaaa agatctggcc gctgtatttc gtactcgtat tgagcttcga caaattggtg
    25261 tccgcgatga agcaaagata ataggcggaa ttggtccatg tgggcgtcca ttatgctgtt
    25321 cttctttttt aggcgatttc gtgcctgtat caatcaaaat ggcaaaagat caaagtttat
    25381 ctttgaatcc aacaaaaatt tccggtttat gcggacgctt gatgtgctgt ctgcaatatg
    25441 agagcgaaga atacgagaca gcgaaaaaag aactccctga ctttggaaaa gtaattgcta
    25501 cacctgatgg taaagggaaa gtcgtagggt tgaatctgct tagccgtatc gttagtattc
    25561 ggctgactgg acgggaaaat cctgtcgaat atgaatggga tgaattgaaa gaatttatcc
    25621 aagtaggtga gatgaatgga taagagatca ttatacgacg gattgaatca attagaatca
    25681 gagttgagaa atacattaac agaacttgtt gaaatgaagc atgctttgca tgagatcgta
    25741 gaaaaaaata caactttgga gatggaaaat caacgtctgc gggaacacct gcgggaattg
    25801 aaccaagttg cacaagctcc agctgaaaat gtgaagcagg aactttctaa gtctcgtatg
    25861 aatcttgaaa aaatttatga agaaggattc catgtctgct acgatttata tggatcaaga
    25921 agggacaatg acgagccttg tgcgttttgt ctagaagtga tctatcgcga aagcggtcga
    25981 tgaccaataa caagaggaga ctggagcgga gaaatactgc tccagtcttt ttattaagga
    26041 gattgtgaca gatgtataat caaaaaagtt tcaagggaaa tccggtatat ggggatttat
    26101 atcttgtacc gacgccaatt ggaaatttag aagatatgag ctacagaagt gttcgtatgc
    26161 tgcaagaatc cgatcttatc gcaagtgagg atacgcgtaa tacacaaaag ttattgaatc
    26221 attttgaaat ccatacaccc caaaaaagtt tacatgaaca taattataaa gaacgtgtcc
    26281 ctcaattgat tgctttgtta aaagaaggaa aaacgattgc tcaagtcagt gacgcaggaa
    26341 tgccttccat cagcgatcct ggacatgagc tagttttagc ctgcataaaa gaagggattt
    26401 ctgtgatctc tatcccagga cctacagctg ggatgacggc tttgattgct tcagggctag
    26461 tgcctcaacc atttttattt tatggcttcc ttccacgtaa gaaaaaagaa cagaaagaaa
    26521 cattgaaaaa gctaaaagaa cagacagtta cgctgatttt ttatgagtca ccttatcgga
    26581 tcactgctac attgacgaat ttcttagaaa tttttggaaa tcgccaagtc gttttgtgtc
    26641 gggaattgac aaaggtccat gaagaatacc ttagaggaag tacggaagag ctactcgctt
    26701 atatcgaaga acatccagta aagggtgaat gttgtctgct tgtagaaggg aatttgttcg
    26761 aaatgccagt gacggaagtt gatgaagagt tgggaacgtt aaaggaacaa gtagaagcaa
    26821 agatcgcttc aggcgaaaaa ccaaatgctg ctatcaaagc agtagcgctg aaaaatgggc
    26881 tgaaaaaaca agaagtctac cgtcagtacc atgaattaga ctgagatagt ataagttgtc
    26941 tataatgaaa gactaatgag gaaatcagct actaactttt caaatctctt gaacgcgaac
    27001 gaatgttctt gtataatgaa taagaggtga ggatgatgga gctgcaattt cctttacata
    27061 atgatacatc aagaaaaatc attcatattg atatggatgc ttttttcgct tcggttgaag
    27121 aacgagatca tcctgagttt cgcggacatc cattagtaat tgccagacac cccagtgaca
    27181 ctggtgggcg tggagtagtg actacagcaa attatgaagc aagaaaattc ggaattcatt
    27241 cagcaatgag tgcacaaaaa gcgtatgagc tttgtccgca agccatcttt aagcctggcg
    27301 accatcataa atacagcgat atttccaaac aagtacggga agtattctat cgttatacag
    27361 atattgtgga accagttagt atcgatgaag cgtatcttga tgtgaccgaa aataaaatca
    27421 attgcaagtc agccatcaaa atcgcccgaa tgatccaagc agatatttgg gagagtgtcc
    27481 agctaacatg ttctgcggga gtaagttaca ataagttttt ggccaaatta gcatcagact
    27541 atcaaaaacc tagaggaatc acggtagttg cccctcaaga cgctgtgcca tttttaaaag
    27601 ctttgccaat tgaaaaattt catggggtag ggaagaaaac tgtacctaga atgcatgagc
    27661 tagggattta tacaggaaaa gacctatatg aatgcacaga aatgatgttg attcgaaatt
    27721 ttggaaaaat gggctattct ctttatcgta aagtcagagg aatccatgat tctccagtaa
    27781 acgttacaag agagcgaaaa tctgtgggaa aagagcatac ttatgggcag ccgttgcaaa
    27841 cggaagaagc agtgctgact cagttgcgcc aactagctga aaaagtagaa gaagcactga
    27901 gaagagtcca aaaacatggg aaaacagtag ttttgaaggt caggtacacg gactattcta
    27961 ctgtgaccaa aagagtgaca ttgccagaat atatctataa aaaagaagca ctcttttatc
    28021 aagcaagcct tatatgggag gaaatcttgg gagtagaaaa aggaatccga ttgttaggga
    28081 ttactttgac gaatctggac ccgatgactt atgaaaacat cgtccttcca ttatgggaaa
    28141 atcaagaaat ataaaaacag ctttgagagt tgaaaccgga aatccgtttt ctctcccaaa
    28201 gctgttttta tatcaagtct tgctttttga tttgttcata ggtttctgtt gcatccgtac
    28261 atttttccca aatcacaact tgattggatt ctaaagattt ttggacgtca atgatccgct
    28321 ggttgctact tccgcgaaat tgtagtttta ggtcacgttt gcttaattca aagcgtccgt
    28381 caacaaggat atcgatttgc gaaaggagtt ctaatttatc ttctgaatct tgtagcaatt
    28441 catcaaaggt atagcctgtc catgaccaga tatcttttgt cgtaccgaac tcttttcgga
    28501 tacgtcgtac gacttgcaga caaacatctg tattcaaaaa aggttcgcca ccaagtaaag
    28561 ttaatccttg cacgtaatcg ttgcgcaagt cagcgatgat ttgttcttct aattcaggtg
    28621 tgaatggttt gccatatcga aaattttgtg cagctttgtt gaagcagcct tcgcaggcaa
    28681 ataagcagcc actaacgtaa agactgttgc ggacaccttc accatcgaca aaattaaacg
    28741 gtttgtagtc agcaatatag ttttggctgt agtcttttgc taaccattct tttggttttg
    28801 gatttctcat aaaaaaactt ctttcattta aggttcaaag atatagctat tgaagataaa
    28861 atctactcga ccacataaat aagaacaaac tgacctaaat ccattgttca agaaagcttg
    28921 ttcttatttt gatagagggc gttcaaagat acaaagccat cgaaagaggg tggtgaaaaa
    28981 agacttgtca tagcctctta ttctgaataa acggtgttca aaagcttgca tctgcagtaa
    29041 taagcccaac taaacgaaaa atatgaggaa ctatttttgt ttagctattc ttattacttg
    29101 atgcaggaca gctttttcac aacctcattt catgtgtttc acacgtgagg aaatttcttt
    29161 atggcggcca tgtaccatcg gacgagcttg cggatttcct aaatagccac aagtacgttt
    29221 gactacatca catgtttttg gatcatgatt tccacattct ggacattcga accctcgttc
    29281 tgtcggtttg aaatcacctt cgaaaccaca ttgatagcaa tgatcgattg gcgtattcgt
    29341 accaagatac ccgattttgt catatgcaaa atcccataca gcttctaaag ctttcggatt
    29401 ttgttggagc atagggtatt cacagtagtg gatgaatcca cctgaacaat attttggata
    29461 atccttctca aattccaatt tttcaaatgg cgttgggttt ttacgcacat catagtggaa
    29521 actgtttgta taatattctt tatctgtgat attttcgatg atgccgaatt tttctgtatc
    29581 caaacggcag aaacggtcag ttagactttc acttggagtt gaatagacac tgaaatgata
    29641 accatattca tccccccatg catctgcatt agcttttaat tctttgacga tatcaagtgt
    29701 gaaagctttt gcttctgggt ttgtttccca ttcgccaccg aagaaagctg aagcaacttc
    29761 atacaatcca atgtagccta acgatacagt agctcgacgg tttttgaata attcattgac
    29821 agaatcggta cgtgaaagac gtttcccgaa agcaccatac atatataaaa ttggcgcatt
    29881 tgctggaatg gcttctttac aacgttctac acgatagaga agcgcatctt tcatgatttc
    29941 taagcggtca gacagtaact gccagaattt attctgatct ccttttgatt cgatcgcgat
    30001 tcttggcaaa ttcaatgtaa caacacctaa attcattcgt ccgacattta cttcttgacc
    30061 attttcgtct ttccaccctt gcaagaatga acgacagccc ataggtactt tgaaacttcc
    30121 agttaattca acgattttat cgtaattcag aatatcagga tacattcttt tcgttgcaca
    30181 ttccaatgct agttgtttga tgtcgtaatt aggatcttca gggtttaagt tcacaccttt
    30241 tttcaaagtg aagatcagtt tagggaagat agctgtccgc ttttcactcc ctaatccatt
    30301 gattcgaatc tgtaaaattg ctttttgaat ttctctttca aaccagtttt ctcctagtcc
    30361 aaatcctaat gaagtgaaag gagtttgtcc atttgaagta aataaggtat tgatttcata
    30421 ttccaaactt tgcatagcat cgaagatatc ttttttggtt tttgcttttg catattcttt
    30481 ttggcgttct ggcgaatcga tccattcttc cgcgtctttt agatgttttt tatagttcaa
    30541 ttctgcaaaa ggagcaagca attcatctgt tcggtcggca gaacagccac catattgact
    30601 tgacgctaca ttggcaataa tctgtgagat ttgtgctgtg gcggtctgga tagattttgg
    30661 cgattcgact tcggcattgc cgattttgaa cccattgttc agcatgcctt tgaaatcaat
    30721 caagcagcaa ttcgtcatcg gtgtataagg atgataatcc aaatcatggt aatggatatc
    30781 gcctttttga tgcgcatttg ctatatgtgg tggcaacatt ttcagtccga ttgattttcc
    30841 aacgatcccg gctgtcagat ctcgctgtgt attgaaaaca ttactgtctt tatttgcgtt
    30901 ttcgttcacg acagtttggt ccttgttgat tagcttttca atcgaaaagt tgatatctgt
    30961 tgctttactg cgagcaaaat ctcgtttggt acgataattg atgtactctt ctgctaattc
    31021 atattcgttt ttttctagta atatatgttc cacgatgttt tgaatctcat agattttgac
    31081 attttcagca aaccggcttt tgatttcttg gacaaccaag tctgcaatca tttgaatttt
    31141 ttcataagta agtggtgata atgagccgtg aattttcttt tctgctttga tcagtgcttc
    31201 attgatcttt tgttcatcga aatcaacatg gcggccatcg cgtttgatga ctttcatctg
    31261 ggatgtctct ggctgctgag taacgacagt tttttcttca taatttactt tcattatctt
    31321 caactcctgc taaattgttc ttgtctatca taaaacaaaa tcggggaagg agcaactaaa
    31381 tatcggctat atatagtatt caatgtgctg tataattgga tttct
//
LOCUS       DAZKOU010000028        30459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000028 DAZKOU010000000
VERSION     DAZKOU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.028"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG827_001180"
     CDS             complement(451..1761)
                     /locus_tag="ACG827_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0483337.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3131)
                     /locus_tag="ACG827_001181"
     CDS             complement(1773..3131)
                     /locus_tag="ACG827_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0483338.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3333..4901)
                     /locus_tag="ACG827_001182"
     CDS             complement(3333..4901)
                     /locus_tag="ACG827_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0483339.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4905..5315)
                     /locus_tag="ACG827_001183"
     CDS             complement(4905..5315)
                     /locus_tag="ACG827_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0483340.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5312..6235)
                     /locus_tag="ACG827_001184"
     CDS             complement(5312..6235)
                     /locus_tag="ACG827_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0483341.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6232..7110)
                     /locus_tag="ACG827_001185"
     CDS             complement(6232..7110)
                     /locus_tag="ACG827_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0483342.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7097..8434)
                     /locus_tag="ACG827_001186"
     CDS             complement(7097..8434)
                     /locus_tag="ACG827_001186"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0483343.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8437..9540)
                     /locus_tag="ACG827_001187"
     CDS             complement(8437..9540)
                     /locus_tag="ACG827_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0483344.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9614..11122)
                     /locus_tag="ACG827_001188"
     CDS             complement(9614..11122)
                     /locus_tag="ACG827_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0483345.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11088..11780)
                     /locus_tag="ACG827_001189"
     CDS             complement(11088..11780)
                     /locus_tag="ACG827_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0483346.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11802..12776)
                     /locus_tag="ACG827_001190"
     CDS             complement(11802..12776)
                     /locus_tag="ACG827_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0483347.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13076..13948
                     /locus_tag="ACG827_001191"
     CDS             13076..13948
                     /locus_tag="ACG827_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0483348.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14406..16604)
                     /locus_tag="ACG827_001192"
     CDS             complement(14406..16604)
                     /locus_tag="ACG827_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0483349.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16836..17828
                     /locus_tag="ACG827_001193"
     CDS             16836..17828
                     /locus_tag="ACG827_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0483350.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17888..19309
                     /locus_tag="ACG827_001194"
     CDS             17888..19309
                     /locus_tag="ACG827_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0483351.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19302..20801
                     /locus_tag="ACG827_001195"
     CDS             19302..20801
                     /locus_tag="ACG827_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0483352.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20840..22234
                     /gene="uxaC"
                     /locus_tag="ACG827_001196"
     CDS             20840..22234
                     /gene="uxaC"
                     /locus_tag="ACG827_001196"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0483353.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22393..23076
                     /locus_tag="ACG827_001197"
     CDS             22393..23076
                     /locus_tag="ACG827_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0483354.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23078..23914
                     /locus_tag="ACG827_001198"
     CDS             23078..23914
                     /locus_tag="ACG827_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0483355.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23925..25679
                     /locus_tag="ACG827_001199"
     CDS             23925..25679
                     /locus_tag="ACG827_001199"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0483356.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25955..26296)
                     /locus_tag="ACG827_001200"
     CDS             complement(25955..26296)
                     /locus_tag="ACG827_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0483357.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26364..28535)
                     /locus_tag="ACG827_001201"
     CDS             complement(26364..28535)
                     /locus_tag="ACG827_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0483358.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28746..29603
                     /locus_tag="ACG827_001202"
     CDS             28746..29603
                     /locus_tag="ACG827_001202"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0483359.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29674..>30459)
                     /locus_tag="ACG827_001203"
     CDS             complement(29674..>30459)
                     /locus_tag="ACG827_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0483360.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9099 a         6274 c         5391 g         9695 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct tgtttttata ttcacaatgt tggcttttta
     3181 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataagatt
     3241 gataactttt tttacataac aatatataca aacaaaaaaa ttcactaact agtgaaaata
     3301 agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc atcatacttg
     3361 aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac tgctatcccg
     3421 ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg acctactttc
     3481 ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt ctttacagtg
     3541 actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat ttgtgctact
     3601 ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca agtaacataa
     3661 caataaatat aattggattc atttaagtac agtaccagtc cagccatctg attaaaatta
     3721 ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg ccgaatagct
     3781 aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg taaaaaacct
     3841 ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc attccagttc
     3901 ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg ttgtacctct
     3961 tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt aagtcttaac
     4021 caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa aatagccgca
     4081 ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg ggtttgtact
     4141 aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc tgtcagcata
     4201 ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca aactgtaact
     4261 gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc gttacgaaaa
     4321 tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt atagatggga
     4381 ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct ggatttattc
     4441 ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc cgtttcaaga
     4501 tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc ggaccaagga
     4561 cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg acggcctgca
     4621 cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat ctgtggcgcc
     4681 catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa aatatccgtt
     4741 gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg aagccattca
     4801 aaagaggaga caataatgta ataccgctct ttcactctta atatattagg atctggattg
     4861 aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc tccttttctc
     4921 attcttactc gatataaact gaatgcccca gctgcgaaat aaaaaaatgc aaaaggaaac
     4981 gcaataaact gagtgccaat caacaatatg aatcctgtta taaaatacaa accagtggca
     5041 gttgttaagt attttccttt agcagtcaaa aacaatgcta ctgctaaaaa agcaagtgaa
     5101 ataaacaaca caacggcaaa cagaccagtc atgtcctgta ataatccaaa tgtattttct
     5161 aaagacaaag ttgaatgatc tacagcaagc gactgataac tttttttaaa ctctgacgca
     5221 gacatattac ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag actgccaccg
     5281 accattccta gcaatagttc ccattttctt ttcatttctg aaccccctgc ttttctaaat
     5341 gtatccattc aggtcttttg atcgatgttt cataattgtt attacgttca gagaatttga
     5401 cagtctgttc atttttcttg tcatcccaat tatcccaacc agttgggtga acatgcccac
     5461 ctatcttaca cgcttcaaat aagacatttg catacggacg ccacggtctt cctaaataga
     5521 cgtttctagt atcaggttct gcagtcaagg tacagctttc aaaacgatac cctgtttttt
     5581 gatttttcgg cgtagatgct gctgtgatgt atccaggatt tttactggct aagctatgga
     5641 tatgacactg atgaaaaacg gcatctgccc caccaaatat gaaatccacc gttccttcaa
     5701 tccagcaata ttcataggtc tgacgacaat actcaggatt ggcagaagcc ggtgtacaaa
     5761 aaggagtacc atctttttgc aaatcaggca acggacccgt gcataaggta tcctgataac
     5821 cagatagtcg acagtttttt aaagatacgc gatctcctga attgaataaa gcaacagcct
     5881 gtcctacctt ttctcctgga cctgctgtat tttcgatgtg aagattttct aatacgacat
     5941 tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc taactggcta ccgtcaagat
     6001 actcctgttt tgcaaaacga ttaccggaga taactactgt tcctgctccc acaagtttaa
     6061 aattggataa tctgctttct atatattcat catatcttcc ttcttttact atcaagacct
     6121 ttggatggct atccggcagg cctgccaaat aatcgattcc ttcttggata gtttcaaaat
     6181 ccgtcggggt atctatagca ttctttccaa ttatcagttt gtctttcatc ctcataaatg
     6241 ctcccttatg aattgtttcc aaatatttct catttcttgt gtttccatat gaccgcctgt
     6301 taaagaaaaa gaagaaaagt tttctggact acccattgat tgatactttt tgtccagccc
     6361 ttttctcagc ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc caactaagct
     6421 aagacgaggg cgcggagcga ttttttcttg gattgctaac gttgtataag ctgtcaatag
     6481 atttggcaca tagtaataaa acccatgatg atctaggcgg cgatgtagca aaagtatttc
     6541 gatgtccacc tgtgccgcta gatcaataca aactttgaca cgagaatcca atgctgtcac
     6601 ccaccagctt aataagcctc ccatagaaag accaatcgtt gcgagccgtt gagtatctgt
     6661 gtcttccctt gtctccaaat aatcgatcaa gctgatcaca tcataaatac gcattcccca
     6721 taacgttttt cctgttaata aaaattgttt gaacaactcg ctttcagaaa tacctccgcg
     6781 ttcttcaaat ccccaagcat caatcgacca aacagcatat cccattcctg tcagtacttc
     6841 tgcaaaagat ggagaagcta agtaaggagc accatggatc aattcacttt tcccgactga
     6901 aaaatctccg ccgtgagaat gcaggtaaag gaccggcgga aacggtcctt ctttttctgg
     6961 ataagcatag agggctggta ctgtttccaa ttcatttaag tacaattgat agtattcaag
     7021 cataaaaccg ttttgttttt ctcggtataa tcgttttcca ctaggggtat gtctgctaga
     7081 tggagcacct aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca accagttcat
     7141 ccatattttc tgattgattt gattggaatt tctcaatact gatttcttta gtattttcta
     7201 atagaaatgc tggtccctcg tgattttcaa tcgacatctg gttaaaggaa accttacccg
     7261 caaagccaat ataaaatcct tgtctgctca tttcctctat tcctgtcatc atagcaggaa
     7321 caccgggaac tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg atttcttgta
     7381 tgaattgttc cgctaatcca taaataaaac ctgcagaagc atggacattc cttgccgtga
     7441 tattcgaaaa atggatcctt ctgaatgctg gtgtccgttc atctatcgga taagcctttt
     7501 tttcccatac atacggttct ttccctcgcg gaccacagaa ataataaaga ttcagtataa
     7561 atggacacat cacattatcc atgactatat tggatactcg aatatcttca acgatcccgc
     7621 ctcgacctcg tctagatttc aatcgtattc ctcgatcagt ttcttggaag atacaatttg
     7681 aaatggtaat attacgaata ctaccactca tttcacttcc taatacgaca ccgccatgtc
     7741 catggaccat cgtgcaattt gtgatcgtga tattttcgca agcgatcctt tcgtacgtat
     7801 cttcagtccc tgccttgatt gcaatacagt catctcccac gtcaatatgg caattactaa
     7861 tacgcacatt tttgcatgat tctggatcaa ttccgtctgt atttggtgaa tccgctgggt
     7921 tcaagatcgt caagttatca aacgttgcat tactgcaaag aatcgggttg atcgtccaac
     7981 ttggtgactg gatcaattta atatctttca cagtaattcg atgacagtta tggaaactca
     8041 tcaatttagg acgaggataa gcgagattat ctggttcatt tctaaaagta tgccaccatt
     8101 tttttccatt tccatctaat gtaccgaaac cagtgacaga gatgttctct acattttgag
     8161 catagataca ggaagcataa acttttcgat ggactccttc ccaacgtgaa tgaacaacag
     8221 gataatcttt tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa tgaagttcga
     8281 cattgctttt taaaaataaa gctcctgtta gaaattcacc tgctggaaca actacggtcc
     8341 cgcctccgtc acttgctgca gcatcaatgg cttgttgtat tgcttcagta ttcaattcat
     8401 caattgatgc gccaaatgtc aaaatatcat acatatttat aatgcctcca cttcaatcaa
     8461 tgcttgcaag aatgcaccaa cgcctttttg atcattacaa ataattggtt cactgatgta
     8521 gtaagcatat gaaccatctc gctgatctgc tccgcctaac cctgctactt gacagttttt
     8581 attcaagttc agccagcctt cttttgtttc cagaacaaat tcatcaagta atccttgatg
     8641 tgatttcttc acttgtggaa gccattcttc tttgttcagc agtcccaaac gtattccttt
     8701 agccattgcg taaacaaaca tactactgcc ggaagcttcc agataatttc ctttccgttt
     8761 tgtttctgtt gttacttgat accatgtccc gctgttttct tcttgatatg gaagtaatgc
     8821 agccagtgtc ttttctagaa tttctttcag caaagaacaa tctgtttctt catgaagtaa
     8881 ttcataggta tctgctgcag ccatcaaata ccagccaatc gatcggctcc aaaaatgatg
     8941 ggatagacct gtttttggat cagcccatgg ttggaccttc ttttcatccc atgcatgaat
     9001 cagcaaattt gtttttggat cgaatagatg tttataactg atttcaaact gtctcaaaac
     9061 atcttggtaa tctttttctt cactaaaatt ctggataaat tcagcataaa aaggagaacc
     9121 catatataag ccatccaacc aaatttgata aggataaatt tctttatgcc aaaaagctcc
     9181 ttcggatgtg cgtggatggt ttgccaactg ctgggaaagg agttctgctg cttttttata
     9241 tttttcttcg cctgtttctt tgtacaataa aaagaaaagc tttcctgtat ttacatggtc
     9301 gatattgtat tcttcaacgc tgtagccacg gatcgttcca tcttcttgca cgaaatagtc
     9361 gatattgtct tttatgaaat caaaataaat tttttcttgg gttcgttccc aaagtaattg
     9421 gaacccttta agtacgacgc catagtcata agaccatttt cctttatacc acttttcttc
     9481 atataagcga ggtgttcttt ccatgatact ttttgctaat ttctttgacc agttttccat
     9541 tatttgctct cctttaaaaa ttaaaatagg atttgggaca aataccaaga agtatttgtc
     9601 ccaatcgtct atattactgt gccgctgctt tgtatgcttc ctcgtactct tgtgtgattc
     9661 gatcgcctcc ttgtgctttc cagtcttcta cagctttttt gaatccatct tcgtcaagct
     9721 gtcccatgat gtattgcacc gtagcatcaa tgacgatttt ttcaatttct gatcctactt
     9781 cattgttcgt cggtgaatcc aatggcacag aaggatccat tacagcaaat tcttcatttt
     9841 ctttgatcaa ttgattagag aattcttttt cagggtctgt atctttgaaa gagaacgtca
     9901 cttcactagg acgagagctt gataacggtt gcacctcttg ttgccaaaga tccatatcga
     9961 taaattcgat agaaccatca tcattcaatt cataatgcgt tccttcgata ccgccagtca
    10021 tcaatttata cacatcttct tctaaaagat catcaacaaa ttgaagtaat ttcttcaatt
    10081 gtgcttcgtc ttttacttga gatttgggaa atgctagcaa gccgccaata ccatttcctt
    10141 ctgaccaaac atgataaccg ccgccttcac catctgagat cttgtttaca ggcactaatt
    10201 ccatatcatc ttgaatacct tgacccatcg tcttcagatt cttgatatcc accattccag
    10261 tgtagattcc agttcttcct tgtgcaaatt gttcttgctg atcagtcttt tgggtaactg
    10321 caaagtcttg tgccaaatag ccattctcaa atagatctct tgaaaaattc aaagctttca
    10381 tatactcttc tgtatcaaat tctggagtga atttgccatt atcgtctaca gcccagccat
    10441 ttggtgctcc aaaataagaa gtgaaaagtt taaagctggt ataacgaata tcactaggcg
    10501 cacgatcgcc aaaaccaact gtgtcgtcca caccatttcc atcaggatcg ttttctgtaa
    10561 attgtttagc tacttcataa agctcatcca tggtagttgg aacatctagt cctagattat
    10621 ccaaccagtc ttttcgaatg actaagccag cacgagcgta gtctttttgt atcggtacgc
    10681 cgtacaaggc accgtcgata ctcgcagctt cgattctatc ttcagaaatt tgacttaaat
    10741 ttttatagtc ttttaaatat tctgatacat cccagaatac gcctgatttt aacgcattgc
    10801 ggacagaaga gtttttcaac atcgcaattt gaagtgtaac gatatctcct agttcatcag
    10861 aagccaatgc agttgttaat ctttcttctt ttgaagcatc aggaatccag ttgaatgtca
    10921 gttccgtatc ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc ggtgaagcat
    10981 gatgcaaaat atttaaccaa gtgattttag cttttccatc ttctgtctct gaagaactgt
    11041 tacttgaact gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta agacctaatt
    11101 ttacgagcgt tgcttttttc ataaattttc tccttcactg ttttaataat atttttgttt
    11161 tcttctgttt tttctgtata tgagttcttc cattccaaat ggcaaaatat agaatttgct
    11221 gtccacgtga ctgcggaaaa gaagagactg ccaccgataa aggttaaaac acctggcatt
    11281 gtttggatga tcatcagata aatccctacc atacttaata tcaacactaa tgagtaatga
    11341 agatgtgaga atccgaataa aaatgccatt ttcattttga aaaaatatcc tttccaatca
    11401 taatgagcca tgattgggaa aatatagagt gctgagagca catatatgaa cgcagcaata
    11461 tacaaagcga tcctaagata ccaattgtgt atgaaagaaa gatccacaag taaaacaatc
    11521 ccgatggcgc catatatcca gctcatcagc aaggtttcac gatagttttc tttaaaatat
    11581 gtgaagtacg ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt tctaatcact
    11641 gcaaccatcg catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa
    11701 ccgccccaca gaaaattcaa ataaaccaac tgtaaaatac gatttagcct tccgtttatt
    11761 ttatctaatg actgcagcat atctttccct ctctttctat cttagtaaac gccgtcttct
    11821 cctagctttt tggcaatctt attagctaaa attaccataa acaatcctac gaccccttta
    11881 aacaatccca ttgctgtact gaaacttaac tgtccattct tcaatccagc tgtataaata
    11941 taggtgtcga aaatttctgc aacgcttcga ttcaatgaat tcaataacaa gtacatatgc
    12001 tcgaacccta agtctaatgt ctgaccgatt tttagaatca gcaaggtaat gattactgga
    12061 cgaattgccg gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc atccatttct
    12121 gcagcttcat acaactgtgt atcgactgca gttatcgctg aaaggtagat gatcgtagac
    12181 cagcccaact ctttccagat cacttgtccg atatatactg tgcgtgtcca ttcaggggat
    12241 gttagaaaac tgattttttc aaatcccatt gttgcaagga attcattcaa tacccctccg
    12301 tcaacattca aaaatacata agtaatcgaa acaatgatta cccaagacat gaagtgaggg
    12361 atgtaaatga tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc atttaatagt
    12421 aaagacaaaa tgattggcat cggaaaaaac acgaagatat ttaatccgaa caagatcaac
    12481 gtatttctta ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa
    12541 ccgacccaag gacttcccat tattcctaaa aatggttgat aatcttgaaa agcaataacg
    12601 agtcccccca taggaagata cctaaaaatc aagaaataaa ggactcccgg cagaatcatc
    12661 acatataaaa atttattcgt tttgattcct gcaattattt tttttcgtct tctgagcttt
    12721 tcttcttttt tcgatagcgt cagttgaaat ggttcttgtt tctttacact ttccataagc
    12781 ccctccttag ttttgtgaat gtttgctgct tatgacttca tgatagataa ctatctgaat
    12841 tacgtatata atcattttta ccgtaagtaa aaacgcttac atagcaatga tttttgtgct
    12901 atacaaaacg ataaagtgta ttttttcaca aataaactac gcatagcaaa ccttctattt
    12961 ttttctatct gacacttagc ataggaaatg attatatacg taatctttca ccgatgataa
    13021 attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa
    13081 aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt
    13141 gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac
    13201 acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta
    13261 tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac
    13321 aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag
    13381 aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag
    13441 cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg
    13501 ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt
    13561 ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg
    13621 tgtatttttc aaaattatgc tgccattagc gttaccgtct attgctacta tctcgctttt
    13681 ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca acgattcatc
    13741 taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc
    13801 agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt
    13861 tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg
    13921 agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc
    13981 agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg
    14041 tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt
    14101 gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac
    14161 ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc
    14221 cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag
    14281 acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa
    14341 atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc
    14401 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
    14461 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
    14521 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
    14581 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
    14641 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
    14701 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
    14761 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
    14821 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
    14881 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
    14941 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
    15001 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
    15061 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
    15121 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
    15181 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
    15241 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
    15301 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
    15361 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
    15421 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
    15481 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
    15541 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
    15601 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
    15661 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
    15721 aatctgtccg aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt taaagacact
    15781 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
    15841 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
    15901 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
    15961 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
    16021 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
    16081 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
    16141 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaatt
    16201 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
    16261 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
    16321 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
    16381 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
    16441 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
    16501 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
    16561 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
    16621 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
    16681 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
    16741 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
    16801 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatgtcgcta
    16861 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
    16921 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
    16981 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
    17041 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
    17101 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
    17161 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
    17221 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
    17281 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
    17341 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
    17401 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
    17461 cagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
    17521 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
    17581 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
    17641 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
    17701 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
    17761 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
    17821 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
    17881 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
    17941 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
    18001 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
    18061 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
    18121 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
    18181 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
    18241 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
    18301 acagagcctc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
    18361 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
    18421 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
    18481 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
    18541 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
    18601 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
    18661 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
    18721 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
    18781 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
    18841 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
    18901 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
    18961 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
    19021 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
    19081 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
    19141 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
    19201 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
    19261 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
    19321 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
    19381 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
    19441 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
    19501 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
    19561 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
    19621 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
    19681 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
    19741 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
    19801 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
    19861 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
    19921 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
    19981 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
    20041 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
    20101 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
    20161 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
    20221 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
    20281 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
    20341 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
    20401 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
    20461 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
    20521 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
    20581 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
    20641 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
    20701 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
    20761 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
    20821 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
    20881 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
    20941 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
    21001 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
    21061 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
    21121 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
    21181 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
    21241 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
    21301 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
    21361 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
    21421 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
    21481 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
    21541 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
    21601 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcgtacaga
    21661 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
    21721 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
    21781 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
    21841 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
    21901 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
    21961 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
    22021 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
    22081 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
    22141 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
    22201 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
    22261 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
    22321 caaatttgct ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa cctttatgaa
    22381 agaagagata acatgcctac tatccatatt gccggtgatt ctactgcggc agaaaaagat
    22441 gaaaacaagc gtcccgagac aggttgggga gaaaaaatct ctgcttattt ttcacccgag
    22501 atccagatca ataaccaagc aaagaatgga cgcagcacca aatcatttat ccaggaagga
    22561 aggttagccc gtctagcttc acagtttcag ccgaatgatt tcttatttgt acaatttgga
    22621 cataacgacc agaaaatcca agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa
    22681 aatctgcaga tattttttga atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca
    22741 tctgtgacgc gacgagatta cttagaaaac ggaacattga atccagatat acttggagac
    22801 tatccaaaag cgatgcgtgc atttgctgag aaacaccata ttcctctttt agatgtgttc
    22861 tcaagatcac aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa attctacctg
    22921 catcttatac ctgatacttg taaaaattac ccagaaggac taaaagacaa tacccacttt
    22981 agtccagaag gcgctgataa agttgcccga ataattgttg aattgataaa agaatcccgt
    23041 ctgcctcttg ctaattattt acaaaaagga gtttgagatg tttccattaa atttaggcat
    23101 ccgtgcccac gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct
    23161 tcacttgtcc catatccaat ttgcaccaca aaaagcattt cctgaacaac ttaaattaac
    23221 tgacatgaca cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa acaatatcga
    23281 actttccatt ttaggatgct atatcaacct atccagcaaa aatcaagaag ttcgtgcaca
    23341 agcattggca actttcaaac agcaaattgc tctgtgctct gcctatcaag ccaagatgat
    23401 tgctacggaa accggtagtg tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc
    23461 ttatcagatc gccagaaatt ctatcattga attagtcgct catgcagaaa attttggcgt
    23521 aactgttgct gttgaagcag ggatcaatca tccaatctat aactatcagt tagctaaacg
    23581 tttaatcgat gaagtagctt caccaaattt aaaaatcatt ttagattgtg cgaatttgat
    23641 gcataaagaa aatcatgctg atcaggaaaa agtgatccga ggtgccttgg acaatttaca
    23701 tggttatatt acagcgcttc atctcaaaga ctatatcatg gaaaatggaa agatccgcat
    23761 ggtacctgtt ggcaagggct gtttagattt ccgcccggta ctgcattata tcaaatatga
    23821 gcgtcctcta atgtacgcca ctcttgaagc tactcctgag atttatgttc ctgaagcaat
    23881 agaacacttg cagtttattt accaagattg ttaaggagaa gattatggag gaacgaatac
    23941 agaaaagtta tcaaaaatat catgcaaatg cttttcagac attgctgcat ctttatcagg
    24001 gaaattattg gcgttttgcc atgtcaggac tcttttttat tatcaaacat cttccatctt
    24061 ggttaatgcc gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc
    24121 agttgcaggc catttatttc aacgcttttt tgatggcttg tctgattggt caaaatatat
    24181 tgacgaatta ttttcatgta aaataccata gcacttcgat ccgtcaagta gaagctggac
    24241 taagagcgac ccttatcaag aaaatccaag aactatctat tccttatcaa aaagaattgc
    24301 aatctggacg aatccagtca aagatcatcc gtgatgtaga agctatccaa acgttgtcct
    24361 ctcaagtatt tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct ttaggtatca
    24421 cgctttataa aagtcctgtt gttttcatgt ttttcttatt gactgttcct ctcgccgcat
    24481 tcgttactct ctttttccgt aaaaggatca gacagtccaa ctcagacttc aggcaagaaa
    24541 tggaagagac ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc
    24601 atgggttaga aaaacacgaa acaactcgct taaataagca ttttactcaa atctatgaaa
    24661 aaggattcca cttagatatc ctgcaatctg ttttcggatc agtcagctgg gctagttttc
    24721 agatatttca gctgatttgt ttgattttca ctgggatatt agctttacaa gggcgcatcc
    24781 aacccggcga tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat tccgtttctg
    24841 gaatgatcac gctgattcct acgatttcaa aaggcatgga atctatcact tctattggcg
    24901 aactgttgat tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc caacatttgg
    24961 atggaaattt tcgttttgag gatgtttctt ttctttatcc ggactcactc aaaccgatta
    25021 tcgatcattt ttctctagag gtgaaagctg gagaaacaat tgcttttgtt gggccatctg
    25081 gctctggaaa atctacactc cttaacttat tgattggatt tatccagccg acttcaggaa
    25141 aaatttttct agatgatcag cctttggatg aactggatat gcgaagtgtg cgaaattatt
    25201 tagccgttgt tcctcaaacc actttgttgt tctccgcttc gatcaaagag aacatcactt
    25261 atggcttgaa aaacgtatca aaagaacgtt tagatgaagt catcgaagct gcgcaacttt
    25321 cttcactgat tgaccagctt cctgatggtc tggatacctt agtaggcgaa catggcaaca
    25381 agctgtctgg tggccaaaag caacgtatct ccattgcacg agcgttgata cgtcagccaa
    25441 aaatcatttt gctagatgaa gcaacctcag ctttagataa ccagtcagag aaaaaaatcc
    25501 agcaagccct gaattacctg acagaaacac ccactacttt tattgtggct catcggcttt
    25561 caacgatcaa agaggcagat aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag
    25621 gtacttacga aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac
    25681 aaaaaatagg agctatgaca agacttttgt cgtagctcct atttctgaat aaacggtgtt
    25741 ctcctgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt
    25801 gaaattgttc cttgtaactc agagttaaac ggtaggctca cgacctcttg gtaaatggtg
    25861 ttcaagagac agcctatcgg caataagaag caattttgtc atcgctcaag gccgaacgtc
    25921 tctttcacac cctctgttgt ttcataaaat agttttattc gctgtaaagg tcgcgaactg
    25981 gtgtcatgat gatacggttc aagtcattga tgattgtact gaagccttgc tctttttgca
    26041 tcaagtcatt gattacttct tctgattgga ttttcacagc catttcttgt gctttcattg
    26101 catcttcgtc gctgaactct tgtccactca tttgtttttc ttgtaattcg atttgcatcg
    26161 cttggaagtc tttgaacagt ttgtgtgctt cttcgtttgc ttgtactttc tcgtacgctt
    26221 ctttcaacgc tttgaattct ggtaattcac ggatctcacg ttcgatttga ttcgcgctat
    26281 cgtagatgtt tgacatgtta tgccctcttt tcttttagta gttactctct attgtaacat
    26341 ggggttattc gttttcaaag gggctactct ccttgatatt tccgccagag gtctttagcc
    26401 gcttcaccaa ttccctgaac gccttctttc accatatcgc cgaagttttt cagcccttct
    26461 tttgcttgtt caccatattg attcaagtta tcttgccaat ttccagactc tccattagaa
    26521 tttcctgtgt ttccaacttg atctgcagct accacttgtc ctcctgtggc ataggcatct
    26581 gctacactaa atcctgtttt tgaagtatac ggaaggatgc cttctgccgc tgatttgaag
    26641 actttcccga caacatttcc acttgtccct tccaaatagt gcagcttgct ggattcttcg
    26701 taacccatcc atgtcgaaat cactacgtct ggcgtgtaac cgatcatcca ttggtcattt
    26761 gcttttgtgg catcgaaatt agtttctgtg gttcctgttt tccctgctat cgtataacct
    26821 gcaggattcg cagcagccgc tgttccattt gaaaatgtac caagaagcat gctggtcatt
    26881 tcatcggctg tttcctttga tatgacctgt tttggttttt tgtttgtgtt atcgacgatc
    26941 accgctccag tcgaatcaac gattttcgtg ataagatgcg gcgtatacat ctgaccatca
    27001 ttggcaaatg cactgtaagc acccgccatg atcaatggag atactccttt ttccagtcca
    27061 ccaagggcta agccataata cttatcagat tttgctaaag gcaggccgaa ttcttctgct
    27121 ttatcatatc ctttttgtaa accgatctca tgcaaaagcc atacagtagg caaattcaaa
    27181 ctttgtgcaa cagcttgata catcggtact tctccttggt atgtcccgtc aaaattatgc
    27241 gcatcgtaat aactttgcgg ttcgtctttc aagatactgg aaggcttgta tccagcttcc
    27301 agtgctggtg tatatacgct taatggtttg atcgttgatc ctggtgaacg acgcatctgt
    27361 gtagcaaagc tgaatcctct aaagacatga tctcctcttc gtcctactag tgcttgtaca
    27421 ccacctgtat tcggatctaa agctactgaa gcagattgga ccatggcacc atctgaagca
    27481 tttgctggga ataatgcact gttttgataa gtagcttcta attgcttttg ataatcctga
    27541 tctaaagctg tatagatttt atagccttta ttcatgacat cttcttcatc aagaccatac
    27601 tcgttaactg cttcatcgat cactgcatca aaataataag gataacgata accagaatct
    27661 gaattatcgt atgtgtcatt aagatacgcg ctgatatctg tctggctttc tgcttgttct
    27721 tgttcttttg tgatttttcc gttttcttcc attacactta gtactgtgtt tttccgatca
    27781 ttcgcatttt ttgggtgatc gatcggatta taaataccag gacctttcag cattcctgcc
    27841 aaagtagcag cctcagacag cgtaacattc gttgcatcta ctccaaaata ctttctggaa
    27901 gcgtcttgga caccccatac cccgttgcca aaataggcat tgttaagata catggtcaag
    27961 atttcttctt tgctgtactt cttttcgatc tcgattgcca aaaataattc cttcgctttt
    28021 cgatcaaacg tttggtctaa tgtcagatac gcatttttgg caagctgctg agtgatcgta
    28081 cttcctccgc cccctgatgt tccgccagac aagaccattc ggactgctgc acgggcaatc
    28141 cctttgatat caaacccgtg atgctcgtaa aaattcctat cttctgtcga gattactgca
    28201 tcttggatat agggagaaat cgcgtttaat tctacatatg ttcctttttg ggcgtacagt
    28261 gtgccagctt cttcgttttt cttatcataa atgactgtcg attggcttaa tcctgatttt
    28321 aacgtttcga catttgcttg ttttgctaaa ataaagaggt agatacttgt cactaagaca
    28381 cagatcaaac caatcagcaa aatcaatttg ttcacatgat attttttcca gatgcgcttt
    28441 cgccatcgat ggaattgtcc gagatatggt ctgatccaac gccaaaaact gatcaaagca
    28501 gcttttatct tttctaaaat gctttgaaaa tccatttgct tcttctcctg tctatttgat
    28561 tcaatcgtct tattttacca tagtgaaaga gtgttttctt cctgcttaag ttgtagttca
    28621 atatgttttt ttacgttagt gcaagtagag taacataaga atgaacagaa aaatgtaagt
    28681 tttttcttaa atatgtttta aaaatggtac aattagtctg cgattgcact gaaaggagta
    28741 cacttgtgaa aatcacgatc actttaccta tgaaccatcc tgaaacaaca attcgcgact
    28801 tgcttgaaaa ggaatggtta gttcctagaa aggttcgaca ctttttgcgt atacgtaaaa
    28861 acgtttggat caatcaacaa cctgctttgt tccatcatga agtaaagggc aatgaccaga
    28921 tcacattgtt attcgaagaa acagattata attatcaaga agttcaatta ggagatgcga
    28981 agcacatccg tgttttatat gaagatgagc atttgattat agtggataag cctgcaggaa
    29041 tgaagactca tccaaatgaa ccgacagaaa acagtacctt actcaatcat ttagctgctt
    29101 atctcgacac taaacagcaa cgaccttacg ttgtccaccg cttggacaaa gaaacaagcg
    29161 gtgccgtttt atttgcaaaa aatccatttg tcttgccgat acttggaaga atgttggaac
    29221 aaaaaatgat ctatcggaga tatcaagcca tcgtatgggg agaaattgcc aaagatatga
    29281 ccatcaccaa taaaattgga cgagatcgcc atgatcggag aaaaagggtc gtagatgata
    29341 aaaatggtca gagcgccttg acacatgtca ctgtcaacaa agtactttct gaacaaacac
    29401 aagtctattg tgttttggaa accggacgta cacaccagat cagagttcat ttagctcata
    29461 agggacatcc gatcgttgga gaccctctct accaaaatag aacagctgaa cgactgatgc
    29521 tccatgcatt agaactgcac atgatccatc catttactca agaaaaaata gtcggtaaag
    29581 ctttacctgg attatgggaa tagaaataaa aaagaacaga gtcttatcag cagataatga
    29641 taagactctg ttctttttgt gtaatttgtc tgcttacttt tgatgttcgt gttctgctaa
    29701 cagcaaacta ccgataatcc cactttcatt cggcagtccc caacgaacga tatattcact
    29761 tacttgaggt gtttccatat aaccacccat caattcaaca aattgctgac ggattttttg
    29821 aaggagatga tcctgattca ttaccccacc gcctaaaata atcttttcag gtgctaacgt
    29881 caacgtatag ttgatcagtg cttgagcaat ataataagcc tgaatagccc aaacaggatg
    29941 atcttgcggc agatcttgcc ctttgatccc tgtgcgtgcc tcaatagacg gtcctgctgc
    30001 taatccttct aaacaatctt tatgataagg acaagtacct tcataatggt catctggatg
    30061 gcgtttcacc caaatatgtc ccatttcagg atgtgcgatc cctgaaaaaa tttcttcatt
    30121 gatcacgaca cctgcaccga ttcctgttcc cacggtaagg taaatacaac tattttttcc
    30181 ttgtgctgct ccttgcttca attccccata tgcagcagca ttgacatccg ttgtccaagc
    30241 agcaggaata ttgtagcgtt ccttgattgt gccaagaaaa tcgaattgtg cccacccttt
    30301 tttgggagtt gccaaaacgt atccgtaatt atcttttgtt ttgtcgatac caatcgggcc
    30361 gaatgagcca atcccgattc ccttcaattc ataacgatca aaaaattcaa atacctgatc
    30421 caatgtttct tctggtgttg tggtagggat acttaattt
//
LOCUS       DAZKOU010000029        28247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000029 DAZKOU010000000
VERSION     DAZKOU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.029"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..923)
                     /locus_tag="ACG827_001204"
     CDS             complement(27..923)
                     /locus_tag="ACG827_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0483361.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(916..1596)
                     /locus_tag="ACG827_001205"
     CDS             complement(916..1596)
                     /locus_tag="ACG827_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0483362.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1597..2379)
                     /locus_tag="ACG827_001206"
     CDS             complement(1597..2379)
                     /locus_tag="ACG827_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0483363.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2372..3289)
                     /locus_tag="ACG827_001207"
     CDS             complement(2372..3289)
                     /locus_tag="ACG827_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0483364.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3453..3707)
                     /locus_tag="ACG827_001208"
     CDS             complement(3453..3707)
                     /locus_tag="ACG827_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483365.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            4564..7791
                     /gene="fss3"
                     /locus_tag="ACG827_001209"
     CDS             4564..7791
                     /gene="fss3"
                     /locus_tag="ACG827_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0483366.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7904..8218
                     /locus_tag="ACG827_001210"
     CDS             7904..8218
                     /locus_tag="ACG827_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0483367.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8231..8605
                     /locus_tag="ACG827_001211"
     CDS             8231..8605
                     /locus_tag="ACG827_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0483368.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8615..8950
                     /locus_tag="ACG827_001212"
     CDS             8615..8950
                     /locus_tag="ACG827_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0483369.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9033..10373
                     /locus_tag="ACG827_001213"
     CDS             9033..10373
                     /locus_tag="ACG827_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0483370.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10451..11125
                     /locus_tag="ACG827_001214"
     CDS             10451..11125
                     /locus_tag="ACG827_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0483371.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11118..11282
                     /locus_tag="ACG827_001215"
     CDS             11118..11282
                     /locus_tag="ACG827_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483372.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11358..12500
                     /gene="dcm"
                     /locus_tag="ACG827_001216"
                     /pseudo
     CDS             11358..12500
                     /gene="dcm"
                     /locus_tag="ACG827_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            12490..12780
                     /locus_tag="ACG827_001217"
     CDS             12490..12780
                     /locus_tag="ACG827_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483373.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13039..14223
                     /gene="mobT"
                     /locus_tag="ACG827_001218"
     CDS             13039..14223
                     /gene="mobT"
                     /locus_tag="ACG827_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0483374.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14220..14357
                     /locus_tag="ACG827_001219"
     CDS             14220..14357
                     /locus_tag="ACG827_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0483375.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            15103..17013
                     /gene="ltrA"
                     /locus_tag="ACG827_001220"
     CDS             15103..17013
                     /gene="ltrA"
                     /locus_tag="ACG827_001220"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0483376.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            17117..17341
                     /locus_tag="ACG827_001221"
     CDS             17117..17341
                     /locus_tag="ACG827_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483377.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            17354..17857
                     /locus_tag="ACG827_001222"
     CDS             17354..17857
                     /locus_tag="ACG827_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0483378.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            17917..18306
                     /locus_tag="ACG827_001223"
     CDS             17917..18306
                     /locus_tag="ACG827_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0483379.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            18293..20740
                     /locus_tag="ACG827_001224"
     CDS             18293..20740
                     /locus_tag="ACG827_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0483380.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            20745..22868
                     /locus_tag="ACG827_001225"
     CDS             20745..22868
                     /locus_tag="ACG827_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0483381.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            22865..23869
                     /locus_tag="ACG827_001226"
     CDS             22865..23869
                     /locus_tag="ACG827_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0483382.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            23888..24799
                     /locus_tag="ACG827_001227"
     CDS             23888..24799
                     /locus_tag="ACG827_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0483383.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            24986..25242
                     /locus_tag="ACG827_001228"
                     /pseudo
     CDS             24986..25242
                     /locus_tag="ACG827_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(25459..25674)
                     /locus_tag="ACG827_001229"
     CDS             complement(25459..25674)
                     /locus_tag="ACG827_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483384.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(25923..26141)
                     /locus_tag="ACG827_001230"
                     /pseudo
     CDS             complement(25923..26141)
                     /locus_tag="ACG827_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(26436..27875)
                     /locus_tag="ACG827_001231"
     CDS             complement(26436..27875)
                     /locus_tag="ACG827_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0483385.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
BASE COUNT         9203 a         5063 c         5915 g         8066 t
ORIGIN      
        1 tactagttct cactaatctt gtataattaa accctccgca acttgacgga gatggagaag
       61 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
      121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
      181 cgctggaaga ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt
      241 aatcgaatga atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
      301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
      361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
      421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
      481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
      541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
      601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
      661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
      721 tcattttcat ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga
      781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
      841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
      901 agaagcaaca gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
      961 agcatgtgcc tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc
     1021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
     1081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
     1141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
     1201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
     1261 taacaaaagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
     1321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
     1381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
     1441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
     1501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc
     1561 gtcttcgata atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa
     1621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
     1681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
     1741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc
     1801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
     1861 tgtcaaagcc actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac
     1921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
     1981 tgtgtaagca agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag
     2041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
     2101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
     2161 cagaagaatc catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac
     2221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
     2281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
     2341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
     2401 gtaagaaata atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg
     2461 cttctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
     2521 cacggctaga ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
     2581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
     2641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg
     2701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
     2761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
     2821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
     2881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
     2941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
     3001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
     3061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
     3121 aacccataat agttctcata aaggtagact tacccgctcc attttcccca atcaatccat
     3181 agatcattcc acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt
     3241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
     3301 tttcttattg atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
     3361 aagaaagtgt aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt
     3421 cctattatca ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc
     3481 aaagtcgttt ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg
     3541 agtaatttta ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt
     3601 agactgtctc gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct
     3661 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt
     3721 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
     3781 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
     3841 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
     3901 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
     3961 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
     4021 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
     4081 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
     4141 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
     4201 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct
     4261 ttacgggctt tcttaagacc tggttttttc ttcgtatcat tgaataagtt tgaaaaacct
     4321 tgatttataa aggttttccc gtaaatatat tttattatat ttgattaaat ttgaaatgtt
     4381 tgtgttctaa aatgtgttct aatttaaagg cagtatattt cgtattttag attaaatttt
     4441 taggctcatt cattctcaaa tccattctat tcagtcaata aaaattttaa aaagacttta
     4501 taaacaaaga tgttcaagcg agcatcttat ttttttgtgc ttaatttgaa aggagaacat
     4561 ttcatgtata aaaaacaaaa aaagaaatta tgtgtattaa ctattttgag tttggctgtt
     4621 caagcaattg ttggagcaat tgctcctacg attacttttg ctgatgaaat tacccatcca
     4681 caaacggtaa ccgtagaact agatttagca catcaatatg ctgttgaagg aacttttagt
     4741 gatggtcgtc caatgtcaga agtcactgtt ccgcattatg cagtgtacaa cggctttaaa
     4801 caagatattt tctgtattga accaggagtc ccgatttaca atgagttcac tcctggctat
     4861 gagaaaaacc cgttaccaga tatgtccgat aaagccaaat tagtttccgt tttatggaaa
     4921 aaagtaggta cagatgcaga tactcatatt gtggcacaga agatgatctg gcaagaagtt
     4981 aatgggtata cccttcactc aataaaacga tcagatggta gtgccgtaaa tatcgcagcc
     5041 attgaggcaa aaataaatca agccattgac gattatcaga agaaaccaac cttccataat
     5101 agtacggcta aaacagtatt agggcaatcg actactgtga cggatacgaa taatctgaat
     5161 ttgtcagagt tcgatgaggt tgtggaaaat gccgcaaaca ttgattatcg tgtgaatggt
     5221 aatcagttga taatcacccc aaatgccaat tcaaaagaaa gtggtgtgct gacgcttaag
     5281 aaatctgctg gtaccggaac acctgtcgcc tataaaaagg ctggccaaca gactttaatg
     5341 gccggggcga ttgataaacc taatacctac acaatcaaaa ttgatgtgga aacggagggt
     5401 tctttaaaga tcaaaaaagt cgataaagaa tcgggtgatg ttgtaccagg aacggttttc
     5461 catttagact ttggaaaaac tttacctgca aaagacgtga ctactgacaa agaaggcatt
     5521 gctacattgg atgagattcc tcatggtaca aaagtaacca ttaccgaaaa atcagtaccg
     5581 gcgccttata cgattgatac cacccccatg actaccacca ttaaagcagg cgaaaccatt
     5641 tacgtgactt caaaaaacgc acgggaaaaa gggcaaatca ttctggataa aagcggagtt
     5701 gaaacaggga gtgatctttg gaatgacaat tattccttag ctggtaatac gtttgccatt
     5761 cgcaaagaca gtccaaccgg tgaaatcgtt caagaaatga ccacggatga aaacggtcat
     5821 gcggaaacac caaaagagat tgctaatgcg ttggaattgg gaacctatta tgtgaccgaa
     5881 actaaagcta gtcatgggtt cgtgaatacg ttcaaacctg taaaagtcga attgaagtat
     5941 gccaatcaaa ccgtagcccc tgttaccagt aacgtaaaag ggcaaaatca agaagtcact
     6001 ggcgaaacca ctttgacgaa agaagacaaa gatactgggg ataaagcaca aggtaaggca
     6061 gtctttgagg gtgctgaata caccttgttt actgctaagg atggaaaagc tgttaaatgg
     6121 agtgaggctt ttaaaccaga gttagcgaag ggaacgaaag gttctgatga aacggtcact
     6181 ttgactttag acgaaaagaa ccaagttgcc gttaaacatt tagctatcaa cgagtattat
     6241 tggcaagaaa caaaagcgcc cgaaggatat agccttgatg aaacaaagta tcctgtatcc
     6301 atcaaaaagg tggatgatag cgaaaaaaat gccgtgatta cccgaaatgt tacggcaaaa
     6361 gaacaagtga ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgcgggcact
     6421 accgaaactg gcttcaatga tctgaccttt aaagtgtcgc cattggaagg gaccaatgaa
     6481 atcacaggtg ccgaagatga agcgaccaca gcttataacg agcaattagg gtttgatggt
     6541 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaagcccca
     6601 gaaggttttc aaaagattac gccattagaa atccgttcta cttttaagga aaacaaagaa
     6661 gactttgtga agagtgagta tgtctttacc attaccgaac aagaccaaaa acaaccaatt
     6721 aaaacggtga ccgttcctta cgagaagctg acgaacaaag cattttctgt tagtttgaac
     6781 cgtttaatgc tctatgattt gcctgaggaa gaagatagtt taacttctct tgcgacttgg
     6841 aaagacggaa acaaagaatt aacttctctg gattctaccg agctagttga taaattgagt
     6901 tacaatctac atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggat
     6961 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtgat tgcagaaaca
     7021 agcgcaacgt tggcgaacaa agagaaaaca ggaacttgga aaattcagca taaattaacc
     7081 gccgaacaag ttttaaataa aactattgtc ttattcaact atgtgtatga aaataaggaa
     7141 gcttttgaag caggcgataa gccagtagcg aaggatgtta gcttgaataa ccaagcccaa
     7201 actgtcagtt gtacagtgga acaccatgtt tctatccaaa caaaggccca cctagaaaac
     7261 ggttctcaga cctttactca tggtgatgtg gtggatatgt ttgatgatgt gtcgattact
     7321 catgatgtac tggatggctc aaaagaagct ttcgaaacaa ttctgtatgc tttattaccg
     7381 gatggtacaa acaaagaaat ttggaaatct ggcaagattg attatgaagt aaatgataaa
     7441 gaattcacca aaactgtgct tgcgaaaaaa gtagataccg gaaaatatcc agaaggaacc
     7501 aagtttactt ttgcggaaat caattacgat aaagatggga atatcaatgg aaaacacaat
     7561 gaagatttga aagaaaaatc tcaaacctta acaccaaaag aagttccaac catactaagt
     7621 acgccgaaac aaccggaaac accaactgtt ccaagtggct ctcaagaatc tagtcccaca
     7681 gtgaagacct tccctcaaac cggggagaaa aattccaatg ttttactgtt cattggtttt
     7741 actttgattt ttgcgacggc gggctattat ttctggaatc gccggaacta aggtgatgag
     7801 atgatacaag taaatcctaa gaaccaacgt gcgcgaccgc ctcctaaaaa agtagtcgcg
     7861 ctatttttgt atccaaaact aacaatgaaa agaggaaaag aagatggaat tgaaatttgt
     7921 cgtgccagat atggctgaaa cctttggaaa gttaagttat gccggtgaag gagaaattat
     7981 aacagaagga tatggacgga atactacggt gattggtcgc agttatcatt tgtattccag
     8041 taagcaacgg gccgatgata ttgaggtggt agtagctgcg gaagccggtg aaaaggattt
     8101 tgatcaagac caaccgctaa aagccgtgaa tccccatttg gttgccaaag gctatgagat
     8161 tgagaatcgt gggtttactg attacgtgtt gtatgttgat gatttagtga aagcatagga
     8221 ggaaaagaag atgagattaa cagaaggtat tgtcgtagat tcaggtttaa catttgggaa
     8281 gttgcgtttt tccgcattac gccgagaagt acggaagcaa aatgaggatg gaacaattgg
     8341 taatgaggta aaagaacgaa cctataattt aaaatcctct gcacaagggc gaatgattca
     8401 agttagtatc ccagctaatg tacccttacg cgagtttgct tatgatgcag aggtagagtt
     8461 ggtcaatcca atcgtggata ccgttgccaa ttatgttttt cgagaaggaa ctaccgttaa
     8521 ttggttcatt aaggcggatg atttagtctt gaagcggcag ccgaatcaag gaaatcctac
     8581 gaaccaaaat gaaagaaaga aataggtgaa gattatggaa attaatgttg aacatatttt
     8641 agattgtctc gatcaatatg gaaaaggcga gctgactgag gaacaactcg ttaaagcatt
     8701 aacctctgat gaaaaaatgt ttctgattat acatcaaggt ttacttggag aaaatagcga
     8761 cagtgaagaa gactctgatg tactagactg gttaggagaa gaagagtctt tctttatagt
     8821 tgtcgaagta aatgaaaact tatgtcggca ggcagaatcc attttggaag aaattggtgt
     8881 ggagatgccg gatgctattg aaggcttttt aaatcaattg gttgaaacga aacaactccc
     8941 ctcctcgtag cagtaaatga ctagcataac tttttgaaat tgcctaatga aatcctattg
     9001 ggctttttcg attggaggtg gaaaccatca aaatgtacaa aggacatcgc atacgcgcag
     9061 gagatcagca cttggtttat cactttgttc ttggctggct actcgctctg ttcatcggtt
     9121 ggatgagcgt cttttatttt caagagttta gacaatttga tatttctaag ctatcacttt
     9181 ctactattga aatcgtttgg tctataaaag atttagtatg tctgcttgga agtctcgctt
     9241 tttcaggagc tatgatattg ctttatattc acttttttct tgatcattgg cgaagcttgt
     9301 ggcatcgaca aaagctagct cggatgattc tagagaacca ttggtatgaa gtgaaacaaa
     9361 ctcaaagtga aggctttttc aaagatttga atagtagccg aaccagagag actatcagtt
     9421 acttccccaa aatctgttat cgaatgaaag atggattact gtctattcgc gttcaaattt
     9481 cacttgggaa ataccaagat cagcttttaa aattggaaaa gaagttagaa agcggactgt
     9541 attgtgagtt agtagaaaag gaactcaaag actcctatgt ggagtatact ttactgtatg
     9601 atatgatcgc caatcgaatt gggatagacg aagtagtggc agaaaatggg actctgcgat
     9661 tgatgaaaaa tcaagtatgg gcttatgatt ctttacccca tatgttaatc gctggtggca
     9721 cgggtggagg gaagacctat ttcctcctca ccattatcga agcactattg aagtcagatg
     9781 cagaattgtt tatcctcgat cccaaaaatg cagatttagc tgatttaggt acggtgatgc
     9841 ctcatgttta ttcacaaaag gaagaaattt ctgcttgtgt ggaagatttc tatgaacgta
     9901 tgatggctcg tagcaaggca atgaaagaaa tgtctaacta caaaacagga gagaattatg
     9961 cctatcttgg acttccaccc aactttttaa tctttgatga atacgtggct tatatggaga
    10021 tgttaacgac gaaagaaagt gcggtgattt tgaataagct caaacaaatc gtgatgttgg
    10081 gtcgtcaatc tggtttcttt ctgattttgg cttgccaaag gccggatgcg aaatatttag
    10141 gagacggaat ccgcgatcaa ttcaactttc gcgtggcttt gggtcgtatg agtgaactcg
    10201 gatattccat gatgtttggg gaggtcgaaa aaaacttctt tatgaagcac atcaaaggtc
    10261 gaggttatgt ggatacaggt ggcagtgtga ttagtgagtt ttatacgcca cttgtaccaa
    10321 aagggtatga tttcttagat tcaattagta aattagaaaa gatgaatgat tgagtgatct
    10381 tcaatactga ctttatactt caagtaacgt ataatacttt tatatagtag gaagaaaggg
    10441 gataattaat atggaaagta atgattctta tttagagtat gcaaaagcaa ataaggataa
    10501 gtttatttct tatattatca aagggaaatc ccccgaagtt ggacgagacg cagtttttat
    10561 ggcagggagt cctggggctg gaaaatcaga ggttgccatt ggattagctg agaattatga
    10621 taaccatgtg attattgatg cggattactt tcgcacccag tttcctgatt ataatggaaa
    10681 aaactctagt ctctttcaaa aagcatcttc ttggttggta gagcaggcat ttagatatgt
    10741 cttagatcat ggctattctt ttattctgga tgcaactttt gcaatattaa gtgctgaaaa
    10801 aaatatctcg cgggcgttga aaaaacattt tcgagtgacg attttctatg tttatcaaga
    10861 ccctaaaatc gcttggaaat ttacacaaga acgtgaaatt gcagagggac gtcatgtgcc
    10921 gaaaaaggca tttattaacg cttttttcaa atcaagagag aacattgcaa aagtaaagag
    10981 taaacatcca gatgtattgc ttcatatcat aataaaagat tatacgaata atatttccga
    11041 agttcattat gctacggata atctagaatt atttttacca ctagggtata gtaggaaaga
    11101 tttggaggtt caattacatg actgagaaag aacaagtaac gaaaattgta aagaaataca
    11161 ataagagcat tgcggattta tcggaaaatg ctactgcaaa agagttcaaa acggtgatta
    11221 aatatgtagc cgatcaggcc aatgaaaaac aacggaaatt agttggatta aataaaaaat
    11281 agtaggacaa attaataatg tatgagccaa gagcaaaatg ttcttggctt ttttctttgc
    11341 aatgaatgag gtaatgtatg agatttttag atttattcgc cggtattggt ggctttcgtt
    11401 taggtatgga acaggcgagc catcattgta tcggtttttg tgaaattgat aaatttgcta
    11461 gacgtagtta taaagcaatt catgatacaa gtaaggaggt ggaaatgcat gacatcacaa
    11521 gtgtatcaga tgagtttatt caatccctcg gaccagtgga catcctttgc ggcggatttc
    11581 cgtgccaagc tttttcaatt gcgggaaagc ggcaaagatt ttccgatact cgaggtactt
    11641 tattctttga aatcgctcgg ttcaccactc ttctccgacc taagctttta ttccttgaga
    11701 acgtccgagg attgctcaat cacgaaggag gggctacgtt cgagacgatc ctccgaacgc
    11761 tggatggatt ggggtatgac gtggagtggt aggtgcttaa ctcaaaggcc tacattcccc
    11821 aaagccggga gcggatattc cttatcggac attctcgaga cgcatgtacc gaacaagtat
    11881 ttcctatcat tggaccgtct tcaacatctg atcaaaacat cagaaatttg gtaaatatca
    11941 atccttccaa tcgaggaatg ggtggacaag tctatggttc agatggcgta gcgccaacct
    12001 taacaggtga tgaaggaatc aaaatcgcac tgccagcaag ggatggaatc tctgtagcag
    12061 gactattacc gggaaagttt gaacaaggta atcgagttta tgaagttact ggaaccgccc
    12121 ccaccttgtc aacgaaacaa ggtggtacaa aaatcatgat tcgaaaaaat gaaacagaat
    12181 atcaagaggt gaagccagaa gacagtgtga atctggcttt tcctaattcc acaagtcgcc
    12241 gaggtcgtct gggaaaacag agtgttcata ccttactaac aggagaccaa caagcgatcg
    12301 tcacggatca gtatcaaatt cggaagctaa cacctagaga gtgttggcga cttcagggct
    12361 ttcccgattg ggcttttgat cgagattctc aagtcaattc agatagtcag ttgtataagc
    12421 aagccggaaa ctctgtaaca gttccggtca tttttgatat tgccagaaga ttaaaagagt
    12481 tggatgtaca tggtttatga tgagttgatt ggagaaattt attgggtgat tggaaagatt
    12541 cagtcagacc cagaattaga agaacaattg cgacggttaa aatttgatat tcgcaagaat
    12601 ggtgtcaaag tcgctggcga tccatattta atgaatgagg agacggatgc tcgaattgaa
    12661 ataaatcaag taattgcaga atttgagcgg attgcggacc aagcaaaaga accagatatt
    12721 cgtcaatact tgttagaaat gaaagcagag ttggaagtaa atggtattac agatgagtaa
    12781 cctaaaccaa cacaactgac tgttaggcgc tgaccgcgga ggattcgcga agcagaaatc
    12841 ctccgtggtg ctgtgcctgt tcatggacgg cgttagccga ccatgatgtc tcacccccgt
    12901 atctaacagg ggggtacaaa aacacaaaaa ataagtaagc aaccattaag aatgcttgtt
    12961 gtatcaaaga tagtaagtaa tttgtatagg tgttaactca tttgaaaaag ttaacacctt
    13021 gaatttggag gtgagaactt ggcacaacgg aatttagatt atcggctatt aaaagaccga
    13081 cgcaacgaat acggcgtttc acaaaataaa ttggctacgg cttgtggact tagtcggccg
    13141 tacttaaatc agatagaaaa tggtggcgta accgcatcta taaaaaccat gaggaaaatt
    13201 tttgaccaac tggaaagctt taatcccgat ttgcctttga cgttactgtt tgactatgta
    13261 aggattcgct ttcccacaac ggatgcacga aaaattattc aagaaattct ccatttgaaa
    13321 tttgagtata tgctccatga agattatgcc ttttactcct atcaagaaca gtatgtcatg
    13381 ggtgatattg tggtgatgtt gtctcatgaa gaagataaag gtgtgctttt agaattaaag
    13441 gggcgaggtt gccgacaatt tgaaactttt ttactcgctc aaaagcgtag ctggtacgac
    13501 tttttcgagg attgtctaaa ggccggtggc gtgatgaaac gcttggactt ggcaatcaat
    13561 gatcgagtgg ggctattgga tatacctgag ctaacgaaaa aatgtcagaa ggaagaatgt
    13621 atctccttgt ttcgtacctt taaaagttat cgttccgggg aacttttaaa ggctgatgaa
    13681 aaggacggca tgggcaatac cttatatatc ggcagtctca agagtgaggt ttatttctgt
    13741 ttgtatgaaa aagactatga acaatatatc aagttaggga ttccattaga ccaaactgag
    13801 acgaaaaatc gttttgagat tcgactgaaa aatgaccgtg cttttcatgc gattcaagat
    13861 ttgttaaaag gtcgtagcat tgagagcacc acgttttcca ttatcaatcg ttacttgcga
    13921 tttgcggata aagtagaagg taaaaggcga accaactggc ccttgaatga acaatggggg
    13981 cgttttattg gacggaatcg gaaagagatt caattaactt ccgagccaaa accgtatacc
    14041 attgaacgaa ccttgaattg gctaggccga caagtcgccc ctacgtggaa aatggctaag
    14101 gagttggatc gattgaatcg gacaacctat atccaggata tggtacagaa tgcacgctta
    14161 tcagaccgac ataaaaagat tttggagcaa caaagtatgg caattgaaaa tttgattata
    14221 tgaaaagagg aatcgtcatg ggaattgtaa aagatatttt tttggtattt ggtggcagta
    14281 tgcttggtgt tactgtgatg tgcttgatga atgcaagtgc agcagcggat cgagcaatgg
    14341 aaaagaaagg aaagtgacaa gatgaatttc ggacaaaatt tatgtgcgac tcgttcctta
    14401 gtgaaaagct aaggcactaa taaaagaacg aaaaagtttg gttattagga gaactgataa
    14461 tctgacaggt ggaatgatga agtaacgctc tgaaacgcct gccctgagcc tccgacaagc
    14521 attgtatgag gaaactcaga atgtttgaag ctcggtaaag acggctgaaa gataccgtaa
    14581 tagttgatga acatatatct tctatcgaaa actatccaga ggtgggtagc gtatccaaca
    14641 ggtcgggggt ctataaaata tctatggtta gaatgttcta taaggaactg acgaaacagc
    14701 gaatgtacgg gtctaaattt gaactttgta gaaatgcaaa ggtacgagaa atcgtagctg
    14761 gtgtggcaaa gtaagaatat aggttatgaa atgccatata acgttacagg cggtatcaag
    14821 ccagcaggct tatagctagt gacctaagga tatatatgta tagataagat tatcggaacg
    14881 aggaaaggta ttgggttctc atcaagagaa taaactgacg aagaacaata agcagttgaa
    14941 ctaatgctga aaagcagagg gcgaacctaa gaaattcttg taatgagaat ggaggaatgc
    15001 cctcaagtcg tgtatatatt agaaaagtat tatgttgttt caataaggat tacgagtaag
    15061 ataaaaaagc tcactcaatt tcaagtaaag gagtgaagcc ttatgcctaa aaataaaaat
    15121 gaaacattgt gtgtagaaga tttaagacac gctgaatatt acgaaatgca aaacaccttt
    15181 gatgatttat atgccaaaag taagaatgga gatattttca ctcatcttat ggatattatc
    15241 ctatcgagag aaaatatatt actggcatat cgaaatatca aagcaaatgc aggcagtaaa
    15301 acagcaggaa cagacggtac aataattaaa gatattggca aattgccagc agaaacagtt
    15361 gtcaaaaaag taagatacat tgttgcaggc agtccacatg ggtatcgacc taagccagtg
    15421 aggcgaaaag aaataccaaa accaaatggt aaaaccagac ctctaggtat tccttgtatg
    15481 tgggacaggc ttatacaaca atgtataaaa caagtattag aaccaatctg cgaagctaaa
    15541 tttagtgaaa acagctatgg ttttagacct aataggtcag ttgaaaatgc aataaaagca
    15601 acctataacc gcctacaaat cagccagcta cattatgtga ttgaatttga tattaagggt
    15661 ttctttgaca acgtgaacca ttccaagctg ataaaacaga tatgggctat gggaatacgg
    15721 gacaagcact tgatattcat tttaaaaagg atattaaaag ctccaatcaa aatgactaac
    15781 ggtacaataa catatccaga gaaaggaact ccgcaagggg gaataatatc cccactacta
    15841 gcaaacattg ttcttaatga gttagaccat tgggtagaaa gccaatggca ggaaaatcct
    15901 gttacaaaaa actatgtggt tcatatcaat aaaagcggta gcccatgtaa aagcaacgct
    15961 tacaaagaaa tgaagaaaac aaagttaaag gaaatgtata tggttcgtta tgcagatgat
    16021 tttagagttt tctgccgcta caaggaaagt gctgaaaaag ccaaaatagc aatcactcaa
    16081 tggattgaac aacgattaaa attggaagta tcacaagaaa aaacaagaat tgtaaatgtt
    16141 agaaaaagat actccgactt tcttggtttc aagattaaaa tgataccgag aagaaagaag
    16201 ttagttgtta aatcacatat aagtgataaa caattcaaaa accaaaaaga caagctggta
    16261 tcacaggcaa agaaaattgc ttctccctca aaagggaaaa cggaattaga agaaacaaga
    16321 ctttataatt caatggtatt aggtatgcaa aattattatc agattgcaac gaatgtaaat
    16381 ctggattgta tgaaactgaa tagagctgta atggctatct tcactaacag attaggtaca
    16441 aacaaaaggg gcagattgag aaaaacaggc agacaactta cgacaaagga ggcaacaaga
    16501 tatggtaaat ctaaaatgct tcggtatgtt gctggcgagg gcgaacctat tttccctgtt
    16561 ggatatatcc aacacagaaa accaatggca aaggtgtatt tagcgaattg ctacactcta
    16621 gagggaagaa aatttatcca caccaatcta agtgttgata aattactgat gtaccagtta
    16681 atgaaattat ctttggaaaa tcgcacaata gaatacgcag acaatcgaat ttcgctattt
    16741 tctgctcaac atggaaaatg tgctataact ctggaagaat ttcaacaagc taatgaaata
    16801 cattgtcatc atattgtacc aactggagct ggtggaaatg acgattataa aaatcttatt
    16861 ttggtaaaag aggcggtaca ccgtctaata cacgctaaaa cagaagaaac aatacaaaaa
    16921 tatatagctc tattgaagct aaataaagtc cagctcataa gactaaataa atatagagta
    16981 ttagctggca atcaagaatt aaacttgata taggtctact acataaatac ttttcaatat
    17041 aaacacgatg gaacgcggag tgcggtgaaa gtcgcacgct ccgtgtgaat ggggggaaaa
    17101 gtttgagata tttataatat caggaactta cctatccata tatcaatggt ttttaaccaa
    17161 tgcccaatcc ttagtcttac tggcaattgt ggtcattggt ttgtttttag gatttaagcg
    17221 ggagttttcc aaactgattg gctttttggt gattgctttg attgcggtcg gattggtctt
    17281 taatgcttct ggggtgaagg atgtcttact cagtttgttt aaccgaattg tcggtgcgta
    17341 agggggaagc acgatggaac agatgcgtgt ttatattgcg aacctcggga aatataacga
    17401 aggtgaattg gtgggggcgt ggtttacccc acctgtcgat tttgatgaag tgaaagaacg
    17461 aattgggtta aatgacgaat acgaggaata tgcaattcat gattatgaac ttccctttga
    17521 gattgatgag tatacgccaa ttgaggaaat taatcggttg tgtggcttag cggaggaact
    17581 tgaaggaacg ccagttggtg aagttgcttc tgagattcaa catgctttct tcaattcttt
    17641 tgaggagatg gtagagcatg tggatgatat tatctattat ccagattgcg atgatatgag
    17701 tgatgtagca tatttcttga ttgtagaagc tggtgatttt ggagaagtac cagaacactt
    17761 gaagacgtat attgattttg atgcgtgtgg tagagacttg gagattggag gcaattattt
    17821 ggtaacgagt cgaggtgttt ttgagtatcc atattaacaa aattattgaa gggtagttga
    17881 tttctaaact atccttcttt ttaaaggaat gatttgatga agaaaataaa aagctacacc
    17941 agtatttggg cagttgaaaa ggtcatttat gcaattaatg actttcaact gccttttcct
    18001 gtgactttta accagatggc ttggtttgtt ttatccttgc tgtttgtcat cgtatttgcc
    18061 catgtgcctc cactatctat gatagagggg gcctttttaa aatacttagg tataccggtg
    18121 gcagttactt ggttcatgtc gcaaaaaacg tttgacggta agaaaccatt gggattttta
    18181 cgatctttta ttagctatca cttacgattt aaggtcactt tcgcaggaaa gaaagtcaaa
    18241 gaacagaaga aacgatggga cgaacccatt actctggtaa ggagtgtgaa ctatgtaccc
    18301 gattaagtat attgaaaata atcttgtctt taatcaagag ggagaatgtt ttgcctacta
    18361 tgagttaata ccttacaatt actccttttt gtcacctgaa cagaaatatc aagtccacga
    18421 taactttcgt caattgattg cgcaaaatcg tgaaggcaag attcatgcct tacaaattgc
    18481 cacagaaagt agtattcgtg ccacgcaaga acgatcaaaa aaggaaatta ccgggcgatt
    18541 aacagaagta gcgaaacaac gaattgattt acagacagag gcattagtat ccatgattgg
    18601 cgatagtcag attgattatc gcttttttat tggcttcaaa ctgattgcga cagatgaaga
    18661 ggtcaatcta aagaatctca agaaatcttt cttctccggc tttcaagaat ttgtctatgg
    18721 agtgaatcat catttgatgg gcgattttgt ttccttatcc aatgaagaaa ttcgccgata
    18781 taccaagctt gaaaaactaa tggaaagtaa attggctcga agatttaaag tcagacgagt
    18841 cacaccgagt gatctgatgt atttgattga acatatctat ggcgaaaagg gtacaccatt
    18901 tgaagaatat gaatttcagc taccaaagaa aaagctgaag tcggaaactt tggtgaaacg
    18961 ttacgactta ttacgtccca gtcgttgttt gattgaagaa aagccccggt acttatgcat
    19021 ggaacatgag aatcatgaat catatgtagc gtacttaaca attaatacaa ttgtggggga
    19081 aatggagttt ccctcctcag aacttttcta ttaccagcaa caacaattta cattcccaat
    19141 tgacactagt atgaatgtgg agattgtgac gaacaaaaag gcacttgcta ccgttcgcaa
    19201 taagaaaaag gaattaaaag acttggacaa ccatgcctat caatctgaca atgaaacaaa
    19261 ttccaatgtc ttagatgcat tggattccgt agatgaattg gaaactacct tggatcagtc
    19321 caaagaatcc atgtacaaac tgagctatgt ggttcgagta agtgccgaat cagtagatga
    19381 actaaagcgc cggtgtgatg aagtgctaga tttttatgat gataccaatg taaaactagt
    19441 ccggcctttt ggcgatatga tggggctaca tgaagaattc ttaccttcta gtaagcgata
    19501 catgaatgat tatatccagt atgttacgtc tgattttctc gccggacttg gttttggcgc
    19561 cactcagatg ttaggggagt tggaaggcat ttactttggt tataacgtag atacggggcg
    19621 caatgtttac ctgaaaccgg cattggcctc acaaggggtc aaggattcag ttaccaatgc
    19681 gctagcagcg gcattcctcg gttcactcgg tggcggaaag tcctttagta acaatctctt
    19741 agtttattac gccgtccttt ttggtggaca agctgtgatt gtcgatccaa aaggggaacg
    19801 gggtggctgg aaagaaacct tgccagaaat tgcggatgaa atcaatattc tcaacttaac
    19861 tagtaagcca aaaaatcaag gattacttga tccttatgtg attatggaga aaaagaagga
    19921 ttctgaaagt ctagcaattg atattctgac ctttttaaca ggaatttcta gtcgagatgg
    19981 ggagaagttt ccagtgttgc gtaaggccat tcggacggtt acccaaagca atcaacgggg
    20041 attattacaa gtaattaatg agcttcgaaa agaaggctca ccagttgcgg aaaatatcgc
    20101 ggatcacatc gagtccttta ccgattatga ttttgcccat ttgcttttct cagatggttc
    20161 gattacccag tcgattagtt tagataggca gttaaatatc attcaagtag cggatttggt
    20221 tttaccagat gcagaaacca cttttgaaga atataccacc atggaattgc ttagtgtttc
    20281 catgctaatc gtcatttcca cttttgcctt ggactttatc catagtaatc gagaaatctt
    20341 caaaattgta gacttagatg aggcgtggag ttttcttcaa gtagcgcaag ggaaaacatt
    20401 atcgaataag ctcgttcgtg ctgggcgggc tatgaatgcc ggggtttact ttgtcacgca
    20461 aaatagtgat gatttactgg atgaacgctt gaagaataat atcgggttga agtttgcgtt
    20521 tcgcagtacc gatattcatg aaatcaagaa aaccttggaa ttctttggct tggatcgaga
    20581 agatgaaagc aatcaaaaac gcctacgaga tttggaaaat ggccaatgct tgatgcagga
    20641 tttgtatggt cgggtaggcg tggttcaggt acatccagtc tttgaagaac tcttccatgc
    20701 ctttgatacc cgtccacctg ttcaagagag aatggagtga ggggatgaaa aagaaacttt
    20761 ttcggattgt gggaatcgtt tttattagca tactcactct gatagttcta ttgagtttag
    20821 ttggtacggt agccgaggcg acagggctag ttgatgatac agttgaagca ggaaatctat
    20881 attccaaata ttcactgaac aactatcaac tagatttctt tgtggatagc tcttgggatt
    20941 ggttgccttg gaattggggt gatggcttag ggaaaagcgt gatgtatggt ctttatgcca
    21001 ttactaattt catttggacg gtgagtttgt atttgtcgaa cgccacgggt tatgtggttc
    21061 aagaggctta caaattagat ttcatttcgg atacggctga aagtattgga aagaatatcc
    21121 aaacattggc tggtattact gaaaatggac tccagacctc cggattttat tttgggtttc
    21181 tattattgat gattttagct ctgggggtgt atgtggcgta taccggatta ttaaaacgag
    21241 aaaccacgaa agcagttcga gccgtgatta attttgtgat gatttttcta ctatcgggtt
    21301 cgttcattgc ttatgcgcct acctatatca caaaaatcaa tgactttagc tcagatgtta
    21361 gtgaagcggc ccttaccctt ggaaccgaga tcgttgtacc caattcagaa agccaaggaa
    21421 aagatagtgt ggatttgatt cgggatagtt tgttttcgat tcaagtccaa caaccttggt
    21481 tactactgca atttgatgat tccaacatag aagaaattgg tgaagaccgg gtcaacaaga
    21541 ttctatctgt gagtccagat gaaaacaaag gcaaagatcg ggaagaagcg gtcaaggcag
    21601 agattgaaga caatgacaat gcgaatctca gtattaccaa gaccatgaac cgtttaggga
    21661 ccgtggtctt tttggtattg ttcaatattg gcatttcctt ctttgttttt cttctaacag
    21721 ggattatgtt gttctcgcaa attctcttca ttatctttgc gatgtttctg ccaattagtt
    21781 tcttattgtc catgttgccg acttatgaaa gtttaggaaa gaaggcgatt attcgtttat
    21841 tcaatacgat tatgatgcga gcgggtgtta ctttggtgat tacgactgca tttagcatct
    21901 ccacgatgtt tttcaatatt tcggcaacct atccattttt catggttgct tttctgcaaa
    21961 ttgtgacctt tgccggtatc tatttcaaat tgggcgacat tatgagtatg ttcaacctcc
    22021 agagtaatga tagccagtca atgggaagac gcgtcatgcg aaaaccgcaa atgttaatga
    22081 atcgaaaatt gcggcaactc aatcgaaatg tgggacgagc tttggctttt ggtggtggtg
    22141 cagcagtcgg aaataagttg gcaaaggaac aatcaaaatc caaatttaag ccatccggtt
    22201 cttctctacg aaagaattca cgattaccga acgatcacga aatactatct gacttaacta
    22261 aggagaatcc agtttcaaat aataagaaac aatcccgaat gaatttggct gggagaaaaa
    22321 tcggcaaagt tttggatacc caagctttgg tgaaagataa gactaaacaa gtaaaggatc
    22381 aggtaaggaa tacgccaacg aatttgaaat acaatctcca taaaggaatc gaaaagtcga
    22441 aaaaagcacc agaagaattc aagcgcggac tgttccaaga aaaagcggat cgagtggatt
    22501 tgcgagaaaa acaacgacaa cgaagaaatg cgaaaatgga cgaaaaacgc aagatactga
    22561 gtgaagcaac aaatcgtcag ggaaaaagaa gggcgaatat tccaatagat aaagcagctc
    22621 aacctcagaa agatatgatg ctgaaaaaag aagcaccaaa gcggattgtg agagcaaatc
    22681 tgaatcccga aatcaaacga aaactaacta gtcaagaaat catttcaaaa gggaaaagcc
    22741 aatcagcaag taagaaaaat tctttccaac aagtgaaaca gaggtccact ctgccaaagc
    22801 agaccaatca aaaagtacaa aaaagaatga accatcgtcc aaaatcaagg tcaggtgaga
    22861 aaaaatgaac ttgaagaaag tgggtgttat ttcattaact tttctgacca tctcttttat
    22921 gggtattttg atgtgtgtgg gtttgttgtt tggtgaagac agtgagggaa atggtagttc
    22981 tggcacttct cctggcggaa ctagtgtctc agaagaagta ttgaaacacc gtgcaatggt
    23041 ggaaaagtat gccaaagaat cggatataac agagtatatt ccaattcttt tggcgattat
    23101 tcaagtggaa tctggtggca caatggaaga cgttatgcaa agttctgagt cagccggatt
    23161 accacctaat tcattaagta ccgaggcatc catcaaacaa ggctgtctct attttgcgtc
    23221 tttggttaag cgttccaaag aactagggtg tgatcaagat agtattattc aggcctataa
    23281 ctatggtggc gggtatctag atcatgtcgg gaaaaacggg aagaagcata gttttgcttt
    23341 agctgaatct ttttcaaagg aaaaatcagg tggtcaaaaa gtcgattacc ccaacccaat
    23401 cgcgattaag gaaaatggtg ggtggcggta taattatggc aatatgtttt actgtttatt
    23461 agtgaagcaa tacctaacta caactcagtt tgatgacaaa atagtccaag aaatttttga
    23521 tgaggcattt aagtatgaag gaacggccta tgtgtttggt ggctcaagtc cagaaacggg
    23581 gtttgattgt agtggcttga cccaatggtg ttatgccaaa gttgacctga aacttcctcg
    23641 ggtggctcag gatcaatatg atgccatgac tcatattgat ctaaaagatg ccaaaccagg
    23701 tgatttagtt ttctttcatt caacctatga tgccggtact tatgtgaccc atgtgggcat
    23761 ttacgcaggg gagaatcgca tgtttcatgc aggagatcca gtggggtgga cgaatctaac
    23821 ggaaaactat tggcggcagc atcttatcgg agctggccga tacaaataag agaaaagagg
    23881 aaaaagaatg aagataaaaa ttgaacggaa tcaaaaggaa aagagcccaa aaaagaaaaa
    23941 agaacgagtt gtatccgtgg gtaagcatcg aaaaatggtg cttgcccttt ggcttttatt
    24001 gtcttgtagt cttgcttttg gcgtttataa aaatttcacc gccattgatc aacatacaac
    24061 tcatgaaaaa gtggttataa aagagaaggt agtgaatact tctggcgttg aaagttttac
    24121 agaggatttt gcgaaagaat atttttcatg gaaaaataac aaagaagcaa ttgaaaagag
    24181 aatgagtaat ctcgaacagt accttacaga aaacggtctc gctttaatcc aagatatggt
    24241 tcgagcagat attcccacga gttccgaagt tcaatctgta aaaattctag atgttgaaaa
    24301 acactcggaa gaatttgtcg tttcattttt agtggagcaa catattacag agggaaaaaa
    24361 gacgcaatca atttcttctg catatcgagt gacaatcttt gaagatgaaa acaggaatca
    24421 tttagtaact agtttgccaa caatgattgg aaagccagac aaggcaaaat atgaagcaaa
    24481 acaagtagaa aacgattcag aaattgatgc caaaacaacg gaagaaatca ccgatttttt
    24541 agaaacattt tttaaacttt atccatcagc ttctgaaaaa gaattggagt attatgttga
    24601 aaatggtgcg atgcgaccaa tcaatattga cttaaagttt tcggagttag agaatccagt
    24661 ttatctacaa aacggaaaaa attttctagt gagtacaaag ataaaatacc ttgataatgt
    24721 atcaaaaacc actagctact ttcagtacaa attagtatta caaaaaaatg gaaactggaa
    24781 aatcgttgat gttaaataat aatgactcaa tctaatttta ttagaagtta taagtagtgt
    24841 aacttatttc aggcagaagt cgcccatcct ctataggtgg gtgatgaatg ccattcggta
    24901 actcgaaaac cgacaattaa gacatagtgc ctttttattt taatccatgt cataatcagt
    24961 ctatatagga aatggttgat ataccatgga attagataca aataatcatt cagtttttct
    25021 tcttggttac ccccttattt tagtagtgaa acattgtaaa catgtcattg atgatgtaat
    25081 gtcagcttat gcaaaaactg cctttgaaag aatttcagaa tcacatcaca ttaccttaga
    25141 tgaatgaaat cgtgataaag aatactttca tattatgctc aaagcacaac ctaaaaccga
    25201 attaacaaaa ttcagcaatg cctaagctca cgtctgataa aatgagactt tctaagggtc
    25261 aaaaatttcc tttgaatacg attattgctt agataaaagt aaaagcctta gaactacggg
    25321 gatagcttgg ttaataagag atatcgttgt tagtaaagac ataggcttgc aagtatgctc
    25381 tattcccaag aatctccccc ttcaagcacc aaggatcgtt aaattcagct aagtggcaag
    25441 taattcacta gacgataatt atctccatat ttcaatctca gcttttccat ttttcatagc
    25501 aaattccgtt atttttatag catctaaaag attcagcttt tccactgatc tttcaccact
    25561 tttcagcttt tttattgtgc ttaaattgat accagtacct tttgatattg aattggaagt
    25621 aaaatcatta agggtttttt ttattaaatt catatccgca attccagcgt tcataagaat
    25681 gaagattctc ctctcataga ctactttgaa aatggtacta cagtggctgt ttatatattt
    25741 cagtaggtta ctgtagtggt catcttatta taattagaaa aggaacctaa atcaaaaatg
    25801 ctaaagaaaa ttttcatagt attaagcctc tcatgactaa agccacggaa ttcttgataa
    25861 aagagtatac ttgcaagcct aaacagcggt atctcttatt aaccaagcta tccccgttgt
    25921 tcctaaggtt cttgatgttt tctaaggcaa tgcttgtatt catagccctt ggtcaaaata
    25981 ttgatactat cgttgacgtt tcgattatga tgtatacggc aaataggaca agtccattct
    26041 cgaattgtga gtgacttctt accatctgtg tgtctacatt ctgaaccaat ttgactagac
    26101 ggaaatcatt tatctatctt aatgattttt tgacagaagt atcgaaaatc tttacataat
    26161 ctgggtgatg tattcttatg cagatgagag cgttggctcc gtattcataa ccagtagcct
    26221 ctaaaacgta gtccatcggt gggataccta gcttgcggtt gccagcggct ttcgcagtct
    26281 tcttgtgatc aaatcaagca tctcattgag cactagtatt ttgaagataa tacttgagtt
    26341 tttcggcttt tagaagttca cttcatcttc catcatccat ggaaatccca ctcacccata
    26401 cgcaggcctc caaaaattct tcaacagtgg ttttttcaga catttttctg aatacctcct
    26461 tgttttttga tacgccagat agtcaggaaa aaggcaacaa aacacaaaac aaagctgcct
    26521 aaaaaggtaa tcttcattcc atataaaaag acatccggcc gatttgcgat atagttagtt
    26581 acttgctggc ccatcttagc agacatcgct tgatataaga ccgtcgtaga aagcgaaatc
    26641 ccgctgacca tccccaaatt tcgggcaaag gaattcatcg agcctgccag tcccaagttt
    26701 tcgatagtga ccgagctcat aaccatcgta ttattggggg actggaacaa ggcattgccg
    26761 gcacccatga tggcagtagc gacaatgtat agccacaaag gagagtccac tgacaaaaac
    26821 atatacatga cctgggttac cgacaataag cccaagccta ctaataccaa aatctccggg
    26881 ccaacccggt cggtcaggta gccgcttaaa ggcgccccga cgaccatcat caaaggaaag
    26941 accatcatca acagcccagc atagctagga ctcaacccgc gagcattttg cagataaaaa
    27001 ggaatcacta cattgacaaa gaagttggaa gaaaaaatca acaaggctgt aatcaagctc
    27061 atggtaaaaa tcttgttctt aaaaataccg aaagaaatca atggagaggc tgctttcttt
    27121 tcaactttta aaaagctgac aaaggcaatc actgaaatta cgaacagtac aagggggact
    27181 ggatggaaaa agccactttc ttgccccaaa aagattgctc caaaaaagct gataatcgta
    27241 gccgcaatgg cagcaaagcc ccctccatcg attgtatggc cgctcatggt gatatctttt
    27301 ggtaagaatt tttcagcaat caacatcgtt acgattccca ccggtacatt gatccaaaaa
    27361 atgtacgccc aagaaaattg ggacaaaatc aagccgccaa gacctggtcc agcaatcgag
    27421 cctaatgaaa caaacgcacc tattgctccc aatgcccgac cacgttcatg tatgggaaag
    27481 acttccgtga tgattccggc atttgtagcc atagtcatac tagcaccaat ggcttgtacc
    27541 acccgtgcga acaataaaat acctagcgaa tgattgaacc cacacaacag cgatcccaag
    27601 acaaagatca gcgtaccaat ccgaaatact ttgattttcc cacggctatc gccaatttta
    27661 ccaaacagca acaaacaaga acagacaatc atcagataaa cggacactac ccattccgct
    27721 tgattcattg gtatgcccat atcatctgag atggtaggta gtgcaatatt gacaatactg
    27781 ccatccaatg tggacatgaa ggtaaacata gatatagcaa tcaaaatcca ccagcgattt
    27841 ttttggactt ttgcatcttc ttgataacta tacataatag gatttccttt ctaatgatcg
    27901 tgtagctaag aacaatataa cctatgtcta ctgcccaaca acttctcgcg actctagcca
    27961 taggaagctt gagtaatttc gatttgatat cctcatgtaa ccatgtaagt tggagatgct
    28021 ttatttgaac ttactctata gtaactagtg ataattcgct ttactttctt ctagcaattt
    28081 ctaaaagtcg acttcattgt tcttcagatt aatagtgtga gctatctcgt gagacgtaag
    28141 caaatttcca ttctactttt tcccaatttt ttcatattta tggtgttgca cctatttgtt
    28201 ttccaatttg cttttcaaat acattgctat gatattcaat tccatag
//
LOCUS       DAZKOU010000030        27474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000030 DAZKOU010000000
VERSION     DAZKOU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.030"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..324)
                     /locus_tag="ACG827_001232"
     CDS             complement(<1..324)
                     /locus_tag="ACG827_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483386.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNV"
     gene            complement(396..1676)
                     /locus_tag="ACG827_001233"
     CDS             complement(396..1676)
                     /locus_tag="ACG827_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFQ0483387.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            complement(1767..2219)
                     /locus_tag="ACG827_001234"
     CDS             complement(1767..2219)
                     /locus_tag="ACG827_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0483388.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(2232..2642)
                     /locus_tag="ACG827_001235"
     CDS             complement(2232..2642)
                     /locus_tag="ACG827_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0483389.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            2917..3129
                     /locus_tag="ACG827_001236"
     CDS             2917..3129
                     /locus_tag="ACG827_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFQ0483390.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            3132..3344
                     /locus_tag="ACG827_001237"
     CDS             3132..3344
                     /locus_tag="ACG827_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483391.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            3484..3639
                     /locus_tag="ACG827_001238"
     CDS             3484..3639
                     /locus_tag="ACG827_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483392.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(3636..3860)
                     /locus_tag="ACG827_001239"
     CDS             complement(3636..3860)
                     /locus_tag="ACG827_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0483393.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3938..4126
                     /locus_tag="ACG827_001240"
     CDS             3938..4126
                     /locus_tag="ACG827_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483394.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            4123..4296
                     /locus_tag="ACG827_001241"
     CDS             4123..4296
                     /locus_tag="ACG827_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483395.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            4265..4393
                     /locus_tag="ACG827_001242"
     CDS             4265..4393
                     /locus_tag="ACG827_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483396.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            4452..4787
                     /locus_tag="ACG827_001243"
     CDS             4452..4787
                     /locus_tag="ACG827_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483397.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            5020..5961
                     /locus_tag="ACG827_001244"
     CDS             5020..5961
                     /locus_tag="ACG827_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFQ0483398.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            5963..6853
                     /locus_tag="ACG827_001245"
     CDS             5963..6853
                     /locus_tag="ACG827_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0483399.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            6896..7696
                     /locus_tag="ACG827_001246"
     CDS             6896..7696
                     /locus_tag="ACG827_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0483400.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7711..8562
                     /locus_tag="ACG827_001247"
     CDS             7711..8562
                     /locus_tag="ACG827_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0483401.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            8628..8762
                     /locus_tag="ACG827_001248"
     CDS             8628..8762
                     /locus_tag="ACG827_001248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483402.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            8763..9224
                     /locus_tag="ACG827_001249"
     CDS             8763..9224
                     /locus_tag="ACG827_001249"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0483403.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            9250..9702
                     /locus_tag="ACG827_001250"
     CDS             9250..9702
                     /locus_tag="ACG827_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483404.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            9808..10002
                     /locus_tag="ACG827_001251"
     CDS             9808..10002
                     /locus_tag="ACG827_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483405.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            10033..10326
                     /locus_tag="ACG827_001252"
     CDS             10033..10326
                     /locus_tag="ACG827_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483406.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            10356..10796
                     /locus_tag="ACG827_001253"
     CDS             10356..10796
                     /locus_tag="ACG827_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0483407.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            10871..11338
                     /locus_tag="ACG827_001254"
     CDS             10871..11338
                     /locus_tag="ACG827_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0483408.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            12085..12315
                     /locus_tag="ACG827_001255"
     CDS             12085..12315
                     /locus_tag="ACG827_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483409.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            12474..13307
                     /gene="terS"
                     /locus_tag="ACG827_001256"
     CDS             12474..13307
                     /gene="terS"
                     /locus_tag="ACG827_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0483410.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            13300..14715
                     /locus_tag="ACG827_001257"
     CDS             13300..14715
                     /locus_tag="ACG827_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0483411.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            14727..16256
                     /locus_tag="ACG827_001258"
     CDS             14727..16256
                     /locus_tag="ACG827_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0483412.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            16174..17226
                     /locus_tag="ACG827_001259"
     CDS             16174..17226
                     /locus_tag="ACG827_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0483413.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            17228..17545
                     /locus_tag="ACG827_001260"
     CDS             17228..17545
                     /locus_tag="ACG827_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483414.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            17672..18295
                     /locus_tag="ACG827_001261"
     CDS             17672..18295
                     /locus_tag="ACG827_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0483415.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            18310..19200
                     /locus_tag="ACG827_001262"
     CDS             18310..19200
                     /locus_tag="ACG827_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFQ0483416.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            19223..19438
                     /locus_tag="ACG827_001263"
     CDS             19223..19438
                     /locus_tag="ACG827_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483417.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            19450..19791
                     /locus_tag="ACG827_001264"
     CDS             19450..19791
                     /locus_tag="ACG827_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483418.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            19791..20162
                     /locus_tag="ACG827_001265"
     CDS             19791..20162
                     /locus_tag="ACG827_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483419.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            20155..20550
                     /locus_tag="ACG827_001266"
     CDS             20155..20550
                     /locus_tag="ACG827_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFQ0483420.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            20552..20929
                     /locus_tag="ACG827_001267"
     CDS             20552..20929
                     /locus_tag="ACG827_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFQ0483421.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            20930..21538
                     /locus_tag="ACG827_001268"
     CDS             20930..21538
                     /locus_tag="ACG827_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0483422.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            21538..21879
                     /locus_tag="ACG827_001269"
     CDS             21538..21879
                     /locus_tag="ACG827_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483423.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            22121..24910
                     /locus_tag="ACG827_001270"
     CDS             22121..24910
                     /locus_tag="ACG827_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0483424.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            24900..25640
                     /locus_tag="ACG827_001271"
     CDS             24900..25640
                     /locus_tag="ACG827_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0483425.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            25637..>27474
                     /locus_tag="ACG827_001272"
     CDS             25637..>27474
                     /locus_tag="ACG827_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0483426.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         9805 a         3955 c         5443 g         8271 t
ORIGIN      
        1 aacgtttttt acaagtacat catcttttat atattttgaa attaatgata gataaacctg
       61 agttgttcga taggaacttt cttttacagt tggcttgtat ttttcatccc atttagtttt
      121 taattctcca aaagttagtg gtaattcttc atttcgtttt attttttctg cgattttttc
      181 attcaaaagt aagttagctt gattccaagc ttggcgtgaa ttgctagata aagtaacgga
      241 cacttttctc tttttttcag ttaagggatc cgtataacgt tcggtgtact taaatttttt
      301 gtcatttatt ttttctaacc acattatttc tattcctttc gaatatacgt tcctattaat
      361 tgttaaaaga aaaagcccga aggcttttta tatatttaat cttttgacaa ttctaaagat
      421 cgtctatatt gttcagcttt agctaatttt gtaaatgtta ctgttccatt ataattttct
      481 aaaacaactt tctctatttc gtctagtgaa actttgaaaa attcttttct ttgattaatt
      541 ttattaactt cttgttttct aaaatgatta tgcaatttag tttccaaaga cggagcgtcc
      601 tctgaaaaaa tcatcgcatg aacgtcaaat tcaaatggaa ccgatgcact actcaattct
      661 tttattctat ccataggttc taatcttcta gtcataccaa ttttatatat gtcttcacca
      721 aaagacccaa tattactaat tacatacaca taaccggctc tagtatttaa ttctctttgt
      781 aatacatttt cacgtataac ttctaattct tttaattttt cttccagttc ttgaatttta
      841 tttgcatata attctttttc cacatctgca ttagcttttt gcatatatgc gattaatttg
      901 gaaatttcag aattgaattg acgttgttct ttatcaatac gtttcttttc acgatcaatt
      961 tctctacgaa ctttttcctc ttctatcatc tgttctttta ttgctttttg ttgttctttt
     1021 tcttcggtaa ttttcactaa ataagagtaa tgaagttcta gttcttgaag tttaaactct
     1081 aaaagaccct tccttaattc agcattgtca ttcttgaata aattattaat tttatcaaat
     1141 atttttatga ttttttccct agtcgagtct atattagaaa tagttagttt attgagtaat
     1201 cctttcactt ctgaatcaaa tggaatgagc atttttttct ttaaattatt tttctctcgt
     1261 ttatttaaaa taagagggac agaaacagca tcgtcaataa tatattcctt ttcttttaat
     1321 tttaacaaag aaagcttatt ttttatttca gaagatgaag tttcttctat atttgaaacg
     1381 ataagtgaag aatagacatt ttttaatgtt tcatcatact tttgatttat ttcatcattt
     1441 aaatcttctt tctctttttt taataaaata atatcatttt ttaacatagt aatttgttta
     1501 tttaaatctg atagtgtttc ttccttactt aatataaatc tttctctatc atgtaattta
     1561 tttagttttt catcattttt ttgttctaat tcataagctt ctttttttat tgtgttagct
     1621 ttcgacagtt tagtgattcc tataattaat aaaataagtc caatcaataa taacattcaa
     1681 aactcctcat ttctttgata taattattgt agagtttact cagaaatgag gaaagagtcc
     1741 atgtgaaagc atgggctttt ttatttttaa tgttgtaata ctaaatgttc aagattatga
     1801 ggaagcccta aatattcaat aatttggaac tgactcatat cagataaatc ttgttcatct
     1861 attgttctag ttaacatttc aattgcaaac atattagctt cagcttctat accataaatc
     1921 atacaactaa ggttgtttgc tctaaaagct gctgtgcaaa tacctttatg gagtctaacg
     1981 tgggctattt catgccataa tacaaatctt ttcatagaag gtgataggtt ttgattaatt
     2041 aatatcacat aacatctttt attgtgtatc gtttgtccta atgttttatt ccctaaatca
     2101 gtttcaatta aatctatatc agaatatttg attattttat atggattaat cgcatcagat
     2161 ccaaactcta caaataattt ttctatttct tctaatatcc agtatttgtt taaatgcata
     2221 ccaaagccca cctattgacg atatttttta ggagtgaatt ttttctttgc tttaactttg
     2281 gttaaggcaa gtgcaagttg aatggcatcg tataattttt ccttatcttc atcgtctaaa
     2341 ggttcaccat aataatttgt ttcagcatct gaattcaagc cttcaagtaa tttttcagct
     2401 tgtaaagcaa tatcttgttt atctttttct gttaaatcat aataatggcg tttatcagta
     2461 cgtcctaaaa gataatcagt tgatacatta aagtaatctg caactttttg tattttatct
     2521 ccagatggag tacttttatt ccatctagac atgcttccat ttccgaaatt taactttctt
     2581 tcaagttctg ccaacgtcat tgaacgttct tttaataaga tttttatacg gtctaatagc
     2641 ataggatatg cattcctttc aaaataccga caaaaaaagt atttttctgc aaattatcta
     2701 tttacaaata gagaatttgc agttatacta gttttgtaag caagatattt acggcataca
     2761 aagactaaaa gcaataaact aatagtctcc ccagacatca gtaagctcgc tttttgtgcg
     2821 ctgtattttg tatgcttaca taatagcata tttgcagttt ttgtcaaact tattagacaa
     2881 aaatattaga aaatattcga taagggggta tgaagcatga ctttaaaaca gcaagtatta
     2941 attgctttag taaagaaagg ttggagtcaa agagagttag ctcgacgaat gggtatatca
     3001 attacttatt tacgagatat cttcatcgaa aaaagaaaac ctaaggaacg tttaaaacaa
     3061 atcgaagaac ttttggatat caagttagaa gtagattcta atgatcagcc atcagaacag
     3121 gaggcgtaaa aatggtacaa cgtttgagca aagcggagtt taaagagaaa tataagtatt
     3181 ctgaaagtac ttatcaaaga agaatgaagg aatttaaaaa atcacgtttt agtgaggggt
     3241 ataacgcagt tacttccaaa gaaataacaa ttgatgtcga tttatatgaa cagtttagaa
     3301 catggcgttc taaaaatcga tttagagtta gagctgtttg ttaatagcta aaaaatctaa
     3361 attcaccact tctgaaaaga ggagttgctg ctctcctttc aagtttgcca aagtccacgt
     3421 tttttcattt tggcaactcc tcttcttgga ggtggtgacg gcgagagacg aaggagggat
     3481 gacatgaagg aagaatcaaa aatagggatt ttgaatgaga acaattacac aagattaatt
     3541 attgaaacag aagaagggaa aaaagtagca gaagttacat tgaccgatgc aactcctgct
     3601 gacggttatc gtattcgatt aactcctaaa tatgattaac cttttggtgg gtgtggatcg
     3661 tttccgtgac tatcacggct ttgaattttt ccatctctgc catgaataaa tagctcagat
     3721 ccttgatttt tagaaatctc tctagcgatt tttactgctt ctgattttgt attagtatgt
     3781 gcagtcgctt tagaatttcc agcacctttg acattccaac caccattgga attaggtaca
     3841 acatgttgat tttttcccat agttttcacc tccatttcta tagaaatatt ttatcaaata
     3901 gaaatggaga aattcaataa acgaaaggag aagtgatatg gcatatacct tgcaacaaga
     3961 acatcaaatt ctcggtttga ttaaacaacg caggaaacaa ttacaagatg accgtgcagc
     4021 gcttagaaaa gccgatgagc tatcagatag gcaagctgaa ctaattgctt ctgaacttga
     4081 ggatttgaga atgctagaaa taaaaaatag ggagattaga ttatgaagaa gacagacaca
     4141 ctttttatag gattcatttt ggggttatta gtgattgtag cgcaccaaag tattatcggg
     4201 ggaagcttgt tcgcagcatt gatggtttta atcaatctgc ttgattcaaa agaaaggagc
     4261 aactatggca cgagaagaag cgctaaaaat cggtaaagtg attgctgata atcggtgggg
     4321 aaatagccgc cctattattt taagcaagca acatatcgaa aagcaaaaag catggcaaca
     4381 aataaaaaag tgactctgcc agccagcaaa gagtcacata caaatttata aaactaggag
     4441 aattatatca catgaataag aaaattgaaa atcttatcga agaattgaaa cgtgaatgcc
     4501 aaaagcaagg tgtttctatt atttgtacag cccaaaaaga aggggaactt aaaagtctcg
     4561 tttatggtga gacaacagaa attttacttt gtcttgctat gcaagaagaa catttagatg
     4621 aaaatctccc attgtcagca catattatgc gtagaattgc tgtggatgct tacgaacaag
     4681 cgaaaaatga agaagaaaat caaccttcaa atcatacttt tgttattaat aacaaggaag
     4741 atttagctga tgtgatgact cgtattctca agggggaatt tcaatgatga atgaaaaaac
     4801 aattgatcta ctcattattt tatttttcac gttagtgatt gtagcgaaaa gttatgtaat
     4861 ctttggcttg ttattcgcaa ttaccatgct tgtgctttgc gtgttagcaa gcagaaaaga
     4921 ggttaaacat gaacgagtta gcaaatcttg acaactattt aactgatcct gattatgcta
     4981 aaccaccata tgaagcacca attgatgagg aggatgaaga tgagtaaatc taccttagaa
     5041 atgagccatc aagaatggct tgaagaccgt aagaaaggca tcggaggttc ggatgttgga
     5101 acaattttag gattgaataa atggaaatct ccttatcaac tatggctaga aaaaacagga
     5161 caagtagtac ttgaagaatc aggaagtgag cccgcttatt ggggtaatgt tttagaagaa
     5221 gtggttgcta aagagtttca ggaacgtaca ggcaaaaaag tacgcagaag aaaccaagta
     5281 tttgaacatc cattacatcc atttttaaga gcaaatattg atcgggaagt agtgggagaa
     5341 aatgccattc tggaatgcaa aacagccaat caatttctcg gtaaagaatg ggaaggcgaa
     5401 gaggtaccgc ttagctatct ctgtcaagtt caacattaca tgaacgttct aaacaaagac
     5461 tattgttaca tcgctgtctt aatcggtggt caaaaattta tctggaagcg gattgaacga
     5521 gatcaagagc tgatcgatac aatcactgaa caattagtag agttttggga aacaaacgtt
     5581 cttggaggta tcgagccaat tattgatggt agtcaagcga cagctgattt cttgaaagag
     5641 aagtatgcag atgttgaaga cgttcaaacg acgttaccaa ttcatttcga tgaactagtt
     5701 gaacagaaaa acgaactcaa acggactaag aaagaaattg aatcagctat ccgtcaagta
     5761 gacaacgaga ttatcagcga gctaggtaaa cgtgaagcca gtatcggtat cactcaaaaa
     5821 aacatcatca gttggaaatt tgtccgtacg agacgtatga actcgaagaa actagcagag
     5881 aaatatccag atgtcgcaaa tgatgaagag atttataacg ttactgaatc aagaaggcta
     5941 accgaaaggg agatcaaata atatggcaac aaatgaatcg ttaaaaaatc aattggcagc
     6001 aaagccacag aaacaagttg caccaggtca attaggactt aaagctttga tgaatacacc
     6061 aacaatgaga aagaagttcg aagaggtgct tcatgacaat gctaatgctt ttatgtcgaa
     6121 tgttatgact cttgtatcta atgacagtta tcttgcagat agtgaaccga tgtctatcat
     6181 gagtggtgcg ttaactgctg caacattaaa tcttgggcta gataagaatt taggttatgc
     6241 atatttagtt ccattcaata gtaaaaacaa gcaaacagga aaatgggaaa agaaagctca
     6301 attcatgctt ggctataaag gatatatcca attagcccaa cgatcaggta aatacaaagc
     6361 attaaatgtg atcgaagttt acgaaggaga actaaaaagc tggaaccgac tgacagaaga
     6421 gtttgagttt gatccaaatg gtagaacgtc tgatgaagtc attggatatg ttggctattt
     6481 cgagttactg aatggattca aaaaaactgt ctattggacc aaacaagaaa tcgaagctca
     6541 tcggattgct aacaataaag atcgcgataa gacaaagtta agtggtgtgt gggcatctga
     6601 ttacaatgca atggcacgaa aaactgtttt gagaaatctt ctttctaaat ggggaatctt
     6661 gtccatcgaa atgcaagaag ccaccacatc ggatgagaga gtccaaaggg ttcaagaaga
     6721 cggcagcatt attgctgaaa cagaagttga agaagatatt cctgaaagaa aagaagcaga
     6781 ggttatatct gaagaaaatg aagatgtaca aactggatta tttgatgcat ctaatccgcc
     6841 gttaaacaaa taatgaggga gctttctccc ttgcttttct agaggagaaa tacgaatgaa
     6901 cacaggatat gtcaaattac atcgaaaagt gatgaattca ttcgtttgga ccaatcctta
     6961 tatgtataaa ttatggaact tgtgtttgat gaaagcgagt catgagaatc gcaagatact
     7021 ttttaatgga aaagagatac aagtgaacag cggagaattc gtcacagggc gcgatgctat
     7081 cacatctgag atgaacgaag gcgtgaaacg tgaacatcaa gtgaacagcg gttctgtatg
     7141 gagatggcta aaacaatttg aaaaaaacgg aatgttgaac atcaaatcaa ctacgaaata
     7201 cagcgttata tcaataaaaa actggtcttt gtaccaaagc agtgaacaac aaatgaacat
     7261 caaacgcaca acaagtgaac aacaagtgca cacaaacaag aatgaaaaga attataagaa
     7321 tgaaaagaat aataaaacat catcgtcgca accacgcaaa aagcgtgttt acgacaccga
     7381 ctcagtttac tacattctcg cggaggagtt attcaaacag atttgtcaga atcaggaaat
     7441 caaaaagccg aatctgcaaa gttgggcaga tgacattcgg aaaatgatcg agatcgacaa
     7501 gcggaccgaa aaccaggtac ggggaatgat tgaatggagt cagcacaatg tgttttgggc
     7561 atcgaatatt ttatctgcta aaaaattgcg agaaaaatac gacacaatgg cagcgcaagc
     7621 aaatcgtgat tataaaacaa aacaaactaa aacgcttgaa tacgagaaat ttagcacaga
     7681 tgagttgcct atttgagagg aggcgtaggc atggaaactg ttggggaaat catggaaaag
     7741 ctgatacaga aagtgcttgt ccaacgtggc gaatgtcctg aatgcggaca gcctttgtat
     7801 ggatggcgta cgaagaaccc tgatggctca gaacgttgta agccaacctg tatgcaatgt
     7861 gggtataaag cattgcgagt tcaggaagac ttacaaacag aacgaattta caacgagagc
     7921 ctgaaagcaa gagcaatcaa ttttttcaaa ggtggttctg ttgtgcctaa tcaagcgttg
     7981 tttgattgca cattgcagaa ttatcaaatt gtcgatcaag aaacaagaca agcggttgaa
     8041 gtaaccaaac gctttgttaa ttcagtcttg ttaggaaatc caagtcacct tgttttaact
     8101 ggaaaacaag gaacaggtaa aagtcaccta gcgatggcag cggcttggga agtcttgaag
     8161 cgatcaaact acgataaaaa aatcttattt atcgggttac aggaaatgct ggatcaaatc
     8221 aaattttcgt acaacaatcc tgaactcaga aaaacgattg agggatcgtt gattgcagat
     8281 atcaaaacag cggatttagt catcattgat gatatcggtt cagaactagg aaaagatgca
     8341 tcagatagtc gagcgtttgg cataaacacg ctaaattcgt tcttggatgc acgacagaac
     8401 ctagcaacga ttatcacaac gaacttgctt ggtgaagaac tgaaaaaagc ttacggtacg
     8461 agaacaatat caagaatgtt tgtcaactct gatggattta cgatggtatt ttctcaaaca
     8521 gcagacaagc gcataaaacc agtcaaagga gcgatcgcat gaatttgact aaacaagaaa
     8581 aaagaatctg tcgtcagata atgttgcaaa ttataggtga tcgtgacatg gacgaattat
     8641 ctcaacaaga gagtcgaatg gttcggaaga taagtaagct aattatttat cgggatacag
     8701 aaagatttct cactgtttta ggtaacgtct acgagaatcc agagttattg gaggtagagt
     8761 aattgccaaa aatattagat gcatgttgcg gtagtagatt gttctggttt gataagaaca
     8821 attctgacgt aacatttatg gattgtcgtc agcagtatga agaattatca acaggtcatg
     8881 tgatcaatgt tgatccagat gtggtcgcag actttcgcga tatgcctttt gaagatgatg
     8941 tgttcgatat ggttgtattt gacccgccac acttaagaca tgtcggcgaa aactcatggc
     9001 ttgccaaaaa atatggaaaa ttagacgaac tttggccaga agacatcagg caaggttttg
     9061 cagagtgtat gcgagtttta cgaccttcag gaacattaat ttttaagtgg aacgaggaac
     9121 agataccact ttctgatgtg ttagaggcta ttggagaaca gccattgttt ggtaataaac
     9181 gaagcaaaac acattggctt gtgtttatga aaaaattcag ctaacggagg aaacaaagga
     9241 ggaaaagtaa tgaagattgt attaaataaa tgttttggtg gctttgggtt gagccatgaa
     9301 gcaaaaatgg aaatttttaa aagaaaaaat attgaagtat tcccatacat caataacttt
     9361 agctatgact cagatgatga atacacgaga tacacaggcc aaaagctagg ttcgatggat
     9421 tttatctact atttcaaaaa agacccgaaa atcgacaaag ttacaggaat ttatagcgaa
     9481 attgaccgac tgtacggaat tgctgatgat tccagttttt caagtgattc aaacagaggt
     9541 gataaggacc tcgttgctgt tgtcgaaaaa ctaggaagtg aggccagtgg accatatgca
     9601 agtcttaaag tcgttgatat acccgatggg gctgagtggg aaataagtga ttatgacggc
     9661 gttgaaactg cacattatgg ttttcaaaca ggtagtattt agtccactaa tgacaactaa
     9721 agaggaggca gaaggatgag tgaagatgaa tactttgaac agtagtctat taacgacaga
     9781 gatttttaac tggaaagggg tgtagtcatg gacgcatcgg aagtagtaaa taaaattaat
     9841 agtctcaaag aggttttcgg agacgtcgaa gtagtgataa acgcacaagg ctatgacaac
     9901 gaatttttta agcagattac cgacattagc attcagcaag gaatggaaga cgaagacgga
     9961 aactttatcg atgaagtagc cattttagtg acttgcgagt aagtaattga cagctaaaga
    10021 ggaggaagcg gaatgaaact aaaagacgga ttttactcca gcggccacgg catcggcggt
    10081 ttaatgctag atatgccgac aaagaatcct aaaacacgta agaaaccaaa attcaaagtc
    10141 ggtgacatgg ttcgctgcga agcagaagga ttcatctatc catttcgtgg atatgtagaa
    10201 cacgtctata atcactcagc aatcattcgc attgaaaaca cgatggaatg tgataagtgg
    10261 ttagcgaaaa gcaaagagaa tttagcagtg gtgagattgg tggacatgga agttataaaa
    10321 tcttaatttt gcttttttac aactatccta caacaatgag cgttattcca caactggaag
    10381 gagaagaggc aattgtgaac attttagaga atatagatat taagcaaaca aggaaaaatg
    10441 ctagacgatt actaaaaaga tacagaaatt tagaacgttt ggtcggacca gtgaaaatag
    10501 acttttccgt gatgactgtt accaaaaatt tgaaattcac aattgacagt caaaacgaag
    10561 aaatcattga agcgataagt actcgagatt cggttatcga agcattaaca cggctaagta
    10621 gaatccattt ccaggtactt tattatagtt attgttttcc taataagatg tcaatgtatc
    10681 aaataggaga aaaattagga tattccgata gaactatcga gagaatgaag gcagtagctt
    10741 tggttgaatt tgctgaggcg tataaatcag gagaactcat ttcacgtaca aaataaaaaa
    10801 agtcggattc ctccgactat gaataatatt tccgacataa gtattatatc ataattgggg
    10861 gaatcagagg atggtacttt tcgatgtaaa gaaatatgag acaccaagcg cgaaagatgt
    10921 tgatatggaa cgcacaaaac ataatgtcgc tgtgtttctt tcagcatatc tatcagctag
    10981 atgtagagta gggcaacatc gagaaccaaa agtgacagca tcttattcct tggttccacc
    11041 ttctacagct gatcatatat ttgaagcaga aagaatgctg atcgataaag aagaagcaca
    11101 ggaagaattt gagtatctgc ataaattgtt tgttcgaggc tattcagcga tacagcatcc
    11161 gcataagcct gatgtgactg aaaggcgcaa gaagatattc tatgatcgtt atatcaatgg
    11221 tctgcccatt tatgtaactg ctcaaaggaa taatactagc gaagaatcgg ttaaagtaga
    11281 atcaaacaga attatcatcc aatttgcttc atcgttagaa ctggttgctt tcaagtagcc
    11341 agtttttaca ctttttatac ccttttatta ccaatttggt ttccatttta tacctttttt
    11401 ataccaatca cttacctatt caacgttgta ttatgatagt gtcgaaaaat taggaaacag
    11461 gatcgacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta agcttcgata
    11521 ggcagcaacg gaaatattaa gaataaggat gtgaatttca actccttcta aattgttctt
    11581 attatctatc atccgttgct gtctattgtt tttttaatta ttcacacgat aactaaaggt
    11641 aagtgaagag aaatgattcc attaataatt tcaatttttg cgctttgtct taatgtatat
    11701 atgattggat ttaaaaatgg gcaaaataaa aaatagtagc agccaagaat aattttatag
    11761 tgtcactgtg gcggaaaggg tagacgctaa gcatgtgtgc taggtcaatg cttcggcaac
    11821 catgcaaggt tcgattcctt gccagtgact ttggcaaccg aggcatcggc ggtttaaaaa
    11881 tataggggtg cgcaatttcg tacgcgtttt gtgcatcgtg taggttgcta ttacataact
    11941 ggttaggtta gattgagatt tgggattcgg tacaaatgaa tcgtcaaatg tctcaagcac
    12001 aggatcggaa acgtccctgc ctgtgcatta catattagat cactctttga gtggtctttt
    12061 tctttacata aaggaggcta cataatgaga aactactggt atgtatcgct aactaatgaa
    12121 tatcctcgaa ccattgatga ttgttcagtg cgtgttgtgc gttctgtaca aatcaaaggg
    12181 aagtactcta ttgtcgaaat ggggagagaa gcagaaccat gtgagattga tgcgtgcaag
    12241 ctcgtttatt gcggtcatgg tttctatgat gaaccaaaca ttcaaaataa tattaacaag
    12301 aatttgaggg attagaatgc aagaaatggc ttaaaaatct ccaatcagga caaatcaagt
    12361 tagacaaagt ttccgattta aagatattga ttgaagctga tttaatgcta aaagatattg
    12421 aaaattagaa aacaaaactc aacctaaaca gaatgtgagg tggtgtgtat tgaatggcaa
    12481 gacaacgtga tccaagacgt gatgaagcca aaagaatttg gttagaatcc aacggagaaa
    12541 agcagttaaa ggaaattgca tctgaattaa atgtttcaga ttctcaggtt agaaaatgga
    12601 aatcgcaaga caaatggagc gctgaattga ttagtaacgt taccaatggc aaaagtaacg
    12661 ttactaatca aggtggcgct cctattggta atcaaaatgc taaaggtaac aaaggaaata
    12721 gccgagcttc tccgccagtg ggtaataaaa acgctttgaa aacaggtgag tatgaaacaa
    12781 tattttttga cacgttaacc gataatgaga aggacatcta ttctagtttg aatgatgatc
    12841 cttcttttgc tttgtccgaa gaaatacgac ttcttaaaat aagacaactt cgaatgatga
    12901 aaagaatcca acaagctgaa gctggactaa atgatgaaga agtcgaacga ttgcagcagc
    12961 taagaaagat taaaactccg attgaaaaaa atggtaaaaa gctagaaatc aagcgtgagg
    13021 ttatgcaaga tgtgcagatt agcagaaaaa aacatcgcaa aattgatgat attctttcaa
    13081 ttgaagattc attgactcgg attagcaacc agttagctaa agccatcaag caaatgaatg
    13141 aactttatat gaatgaatac agaactgatt taattaaagc tcagactgat aagatccaag
    13201 ctgagacaaa tgaaattggc ggaaataatt caggtgaaga aatagaagaa tggaaacagg
    13261 cagttttaaa tgccgcaaac aaacgggcgg tgaaagaaaa tgaataatga atttattcct
    13321 tttgctgata ttggttctgc cattgattat tactatgata aaccagtagc tttttgccaa
    13381 gatattttgc atttgaatcc tgatgaatgg caagaaaatg ttttaaatga tttagctgaa
    13441 ttttcaaagg tttctgttcg ttctggtcaa ggagttggaa aaacagcatt agaagcagga
    13501 gcaatacttt ggttcttaac gtgtcgaccc tacgctaaag taatagcaac agctccgaca
    13561 atgaagcaac tttacgatgt actttgggca gaggtagcta aatggttaaa tgatagcttg
    13621 atcaaaaact tactgaagtg gacaaagacc aaaatttata tggttggtga ttcagagcgt
    13681 tggtttgcta cggctagaac agcgactaaa ccagaaagta tgcaaggttt tcacgaggac
    13741 catatgttga ttgtggtaga tgaagcttct ggtgtgtctg atccaattat ggaagctatt
    13801 cttggtacgc tatcaggttt tgataataag ctgttgatgt gtggaaaccc caataatatt
    13861 gaaggtgtct tttatgattc ccataattca gaccgtgata aatacagagt tcataaagta
    13921 tcaagctatg atagtaaacg tacaaacaaa gacaatatag aaatgattct taaaaaatat
    13981 ggaaaagaaa gtgatgttgc tcgtgtccgt atttttggag aatttccgaa aggtgcgttg
    14041 gattcattta tcagtcttga aacagttgaa ttggctacag aaaaacaaat tagtgattct
    14101 ttagtcaata aaacaaaggt tgctcatatt ggtgtcgacg tagctcgata tggtgatgat
    14161 tctacgattc tctttcctag aattgctacc agggcattgg agtatgagaa gtattcaaaa
    14221 cgtagcacca tggaaacaac aggatatgtc atcaacatgg ccaagaatct aatgagtcaa
    14281 tatccgagta ttgataaagt gatgattaaa gtcgatgaca ctggtgtcgg aggtggtgta
    14341 accgaccgcc tagaagaact tatagaagac aaacattatc cttttgaggt gtttggagtg
    14401 aataacggtt caacatcaga agacgatttt tacgataatt taggtactca actatgggga
    14461 aacatcaagg aaatgttaga agaaaatatg acagcaaatc ttaacggaga acagcctgtt
    14521 attgaattgc cttctgatag ttcgttaatc aaagaattaa gtactcgcaa attcaaaatg
    14581 acaagtagaa gtcgtatacg tttagaaagt aaagatgata tgaaaaagcg aaatattggt
    14641 agtcccgata ttgctgacgc actggcttta gcgttttatg agccaccaag tcactatcaa
    14701 tttattcaat tttaggaggt gagcttttga ttagtacagt attatcttta gagagatata
    14761 agaagctacg tgttaaatat gcaacacaaa ttgaagatgg aatgtttgat ccgaatggtt
    14821 ttatagaaga tatgaaacca ttttttgctg atcgcgaaag aaaatatcta gcttatacta
    14881 gcgaaaagaa tgaaatagat aatagaccaa agcctaacac tgatattgta aaagttaata
    14941 ataaacttca tgctggtatg tattcaattg tcgtagacca agcagtcaat cattttactg
    15001 gtatacctat caaatgggat tacgatgtat cggaacaaaa aagaacactc attcaaagat
    15061 taaaagataa attcttaaaa aatgatatag aacttccgac agttccagaa gcttttaaca
    15121 aattaacaag caatcttgat tccatgagat tcgcaatgct tgattctgaa actgcaactt
    15181 ttcaaggagc ttgtggagta gctttccgat tgctagagcc tgtggaagaa gatgatggtt
    15241 ggaagttaag agcaagtaat attgaacctt ggagagcaga aagatatgga aatgccggaa
    15301 tctatatcaa agaaaagtat gactcttacc agaaaaaatt ttttcaagaa atgaaagtta
    15361 tgacaagaaa taaaattctg acatatgctt gctatggtga tttgaatttt gttacaagcg
    15421 gaacgttcaa aaaaattgat gagaccgata accctttggg gacgattacc ttggcagaat
    15481 ttaaaaataa tacgaatcgt tattgtgatt ttgaagtagc tgaggaaatt ggagatgcaa
    15541 ttgatcgtgc tttgtctgat caacaaaacg aaatcgaaca atttaagctt gcgtacatgc
    15601 ttgttactgg cacaacaatg agcaaaggca ctgctaaaga aatgatgaat cagctaggga
    15661 ttatcaactt aaaagcccct actgcaaaag ctgaatatgt cacaaaaaat ttagcaaaag
    15721 atttcaatga atatcatatg gatctattga aaaaacagtt ttacaccatt tgtaaagcaa
    15781 tcgattttaa cgatgaggtg tttaaatcaa acagttctgg agaagctcgc aagtggcaaa
    15841 tcattagcct agaagcgaaa acaaatacta aagagcaata ttttagagaa ggattgaaag
    15901 aatgcgcaga aacaattgct gcttttctta agtttcatga caaagtagaa attgagcctg
    15961 aaaaaattat ttttactttc tctagatctt tgccaactga tttaagttat ttagctgaag
    16021 ctttacctaa acttgcacca tacgtatcca aacgaactat tcagagccaa attccatttg
    16081 taacagatgt tgattatgaa aatgaaatga tggaattgga aagtggaagt gcttatccag
    16141 atagtgaata caattttggc ggaggtggca atagtgacga tagaaacgaa gtattggacc
    16201 aaacgtcgag aactggagga tcaagcaagg ctcaaacaag aaaatcagac acttaaaaaa
    16261 ttaactagtg tatttcctga agcactgaaa gagatacaag caaaactatt atcacaagct
    16321 gacttacaca atatcactta tccagaaatg atggagtttt acagtacaag taatcagaaa
    16381 aaatatcgtg aatatgtgga aaaaaattat aagtcattaa aaatgtatga tgcaaaatac
    16441 aaagagttta tcgatgaatt ttttccacca tttgactatg caaaagtcaa tcgcttatta
    16501 caaatacgat cagatgtatt taaaattctt gcagaatatg cgatggatgc agatgtgaat
    16561 caatattttt ctgatcgctt agaggaaatt cttcaaagaa catattcttc taatgctaat
    16621 gtttttgttc aacttttaaa cgttgatata ccaaattatt taccagaaaa tgaaattaaa
    16681 cattatttga actatccatg gtcaggaaag acattttcaa gaagactttg gggaaatatt
    16741 tcatcgcttg aacagaaact ctctaatgct attgtaaaaa gtgttgctag tggagaaggc
    16801 gttatacacg cattaaacac catgagatta gactcagaaa tttgtgacat gtttaagtta
    16861 gaagaatcaa agtataacaa agcgatagaa aatctcgttc gaacggagta tgcaaaattt
    16921 gcacaagatg gtattgaaaa atcatattta gaaacaggta ttgaggaata caacgtattg
    16981 actgcaaaag atgagagagt ttgccggatt tgtggaggaa aggcaagtaa gaatccctat
    17041 aaactgaaag atgctgtcat aggtgaaaat cgagcacctt tccatagtcg ttgtagatgt
    17101 acggatgttc ctaatttacc aaaattagga aaggatattg atgaagaata tgaccgttta
    17161 tttggcgatt tattagatga gtttgcacat gattcttttg gaattaattt gaaacggagg
    17221 aagtagaatg aaagattttt ttgaagcagt actaacaatt aatgtaaatg ctgatattgc
    17281 agaagcctac aaaacagcta ttgagtctga gaaccatcct aatggcttga gagaccattg
    17341 gaatggcaat tatgcctacg tggttattgg cgatcaaact gttaattatc aagataatac
    17401 tccagttgat aagaataccg ttaatttaac gattcaatta ttatctcatt cattaccaaa
    17461 tttaaaagaa acagttgatt ggtatgaaaa gatgggatgt attgttgtta gaactgacta
    17521 caaagaagga aagtctagta attaggcttt tttattttgt ccgaaatgac gttaaactag
    17581 cgcaatactg ggcttggttg aatggtgggg cgcaactatt aaaactcaaa gcaatgcggg
    17641 gcgtgaaaac gaatcgtggg gcgaaaggag aatgagcatg aaacacaaat cattaatgcc
    17701 aatgaatttg caatactttg ccgaaggtga cgatcaaaag ttttcttttg atgacttcaa
    17761 atcttttgtg gaatccaatg aagaagctca aaaatttatt cagtcacaat ctcaaagtgt
    17821 tgccgacaaa caattggaag cttggaaaca aaataactta gataaaatta aacaggatac
    17881 catcaaggaa tatgaggaat ctaagaaaaa caagtcacct gagcaaattc aactggaaaa
    17941 actacaagct gaatttgaag cagaaaaagc gttgcgtgtg acaagtgata ataaagcatt
    18001 tgttgcagaa caaattgctg gattagaact agatggagag ttaaaagagt ctatttctca
    18061 atttatgcta aataatcttg ttagttcgga tacagatttc actaagaatg ctgttgaagg
    18121 ttttacaagt gttttgaatg caattaatga gaaacatgca gatgcattaa aagaactaca
    18181 aatgaagtct gcatttggtg gaactcaaca atcgaataac caggttcagc agaacaatga
    18241 aacattaaca aatccagaag aacaattagg acaaattctt caacaattta actaggagag
    18301 tgaaaaatta tgaaaaaaac atctttaaat aatttagagt atttggatat ttcaccagcg
    18361 attaatgcta tgcaagtacc aaatacacct tttttaagct atttacttgg tgctggaaaa
    18421 acagagccag caaactcgac agaaattaaa tggcgtgaat atgatatcaa caacgatgat
    18481 tcttctgaaa aacttgaggg cggagaatat ccagatgctg aatcaggtcg aacttggttt
    18541 aacaactata ctgaaatttt tagaaaatca acctctgtat ctggtacatt agatgctatt
    18601 aatgtgaatg gagtcggaaa tgaattaact aatcaagtag cactacgtgg tatggaaatg
    18661 aaaattgatt tgaaccgaaa attgattact ggtgtaaaag ctgatgaaaa tggttctaaa
    18721 ggtcgtcgaa tgaatgggat tttgaacttg atcaattcag caaataaggc agaaacagcc
    18781 actgcgggtg cagtaacaag aaaagatatc gatactttat ttaaattgat gtatgaaaaa
    18841 ggttatatgg gagaaaaact atgcttgatt tctccagata tgcaggagtt aatgactgat
    18901 gagttagatg gaaaatcaac aaaaattgtt cagttcgggg aaagagtaac ttttggattg
    18961 caaattggaa atatcgtgtc taattacggt acaggtattg ctctactaga accatcattg
    19021 ccaaaaggaa caattgccgc aatcgatact aattatgtga aactacgtcc attacgtgaa
    19081 tggagagcag aagaacttgc aaaaacaacg gattctagac gtatcggtct tgtaggtgaa
    19141 tactctcttg aatacaatgc ttcaaactct ggggcaattt taaatttaaa gtctgaataa
    19201 aagggagtta gtactccctt ttttgatagg aggaattaca gtggtaaaaa aagatgaaac
    19261 taaaaaagat gaagtcgtga aatatagagt aggtaaaact aaaaattttg ttgggtttgt
    19321 tcatcctaaa actcgtagat ttatcacagc agattcaaat aacgaattta tcatttctat
    19381 agatgataaa gaagcaattg caattttgga agatgcaatt gatgttaatg aaatttagga
    19441 agtgatctga tggatgaatc gctaaaaacg gaaatcattg agtctacaaa agaagatttt
    19501 ccagatttga gtgaagaacg cataactaat ttattagaaa taattttgct agaaattgaa
    19561 tcatacaaca cttgtaaaaa tgatatttca tgggaaaagt taaaaagcgt gattagtgaa
    19621 gtgttgtata aaataataaa aaatgaatca gaaaaaacag tatcttcagt tagacgtggc
    19681 gatacaacga ttagttatgc ttcaacaaca aatgatgtta gtgagttact tctaggctat
    19741 ggtgatttga tacgaagagt tattggttgt ggaggattgg agttttttta atgaatgaag
    19801 cagatatttt agaaactact tatgaagata gttgtattat tgaaagactg acggacattg
    19861 aagattctaa tacaaatatt actattcaag attacaaaaa agtatatgat aatcctattt
    19921 cttgcgctct ttcacaaggt caaatcgatg gactagcagt catagaagat ggagagatgg
    19981 taaatgtttc aactgacaca tataaattat ttgttcatcc taagattaaa ctaaaaaaag
    20041 gagatcgaat aacaataact caaaaggcca gtggcttaat tttttctcta tttgctacta
    20101 agccttttta ctatcctagt cattgtgaag tgaatttgat aggaagtgag aaaaatgggt
    20161 aatctcaaat atgaatcaaa tgcagaaaag atcattgaga attttaaaaa tatgactgta
    20221 attgctcaaa aagaaggaat atccttcgtt aacgattcaa tgaataaagt cgttagttta
    20281 gctaaaccat taactcctgt aaaatcaggt aatttaaggc gaggatatcg tgtagtaaaa
    20341 gctagaaaac tatcaagcgg tcgaattgtt ggagaagtta taaacaatga acattatttt
    20401 aaatatgtag aagaaggtca taggactaaa aatggtggct ttgtcaaagg gaaattcatg
    20461 ttaactcgtg caacgaatct tgcaaatatg tcttatattc ctcgaagatt taaacaaatg
    20521 gcaataaaaa tcgttaagaa aggaaagtaa catgaaagat gaaatcattg ctgcaatcag
    20581 tagcaaatta aaagaaatct atcctgatgg gacaatatat cttgattcag ttatgcagtc
    20641 aactaaagat ttttatttcg ttttatcagt aatggaatct ggaactgaaa atgtaggaat
    20701 tgatattcaa aatgtttctt tcttaattga tattgcattg attgataata accctaatag
    20761 aaatttaatt aatgaattag tctcacgttg tgggactttt tttaatacga ttacaatcga
    20821 tgagcaaata ttatttccaa aagcctattt acctgatgaa gcagatggtg ttcaacatat
    20881 tcgttttaca ttagaatttc cacaatatat tgaatggagt gaaagataaa tgggagaaaa
    20941 aagaagtaaa accggaatta tttctgtcga aaaacctact tggtttcctt tggaagatga
    21001 gacagggaat tttccggtat atggtactgc gttgccaatg ggaacagcag taagtatcaa
    21061 accaacagct aattatgaaa caacacaaga ttatggggat tcagtggttc aagatcaatt
    21121 tacagctttt ggtggtgcag aagttgaact ggaagcaaat ggatatactc ataaagtttt
    21181 atctacaatt actggaggga aaattgttaa aggtggtgcc ttgcgttctg gagaagatat
    21241 tgcgcaagat ggtgcgtttg catatagacg taaaaaatcg aatgggaaat accgttatac
    21301 ggtcttttat aaaggacagt ttgctctaga ttcagatgaa acatcaacga ttgaaggaag
    21361 taaagtaagt tttactcatc caacttggaa gggttcattt gtagatgtac caggacttgg
    21421 atatatgtat tcagttgatg aagatgatga aggcgtggat aaagcaatga tcggaaattg
    21481 gtttacaaaa gtagcaattc caattgaaga agcagaactt tcaggaggta cagaataatg
    21541 tcaaaatatc aaacaacgat taaattaacg aaaaaagacg aagaaggtaa atatgagcaa
    21601 gtacaattta aatctgctga atttttacca ggaacagttg tagaagatgc tgcaggagtt
    21661 atggaagaaa tgcaaacagc gactgataag caatcagtta aaaaagcttt aagtcgtgct
    21721 tattcattta ttgcagatac tttatttgag ggacaattta ctggtgaaga ttattgcaaa
    21781 ggaattgatg ctcgtgagat tgcctcattg acaggaaaat tattgaagtc tgttactgca
    21841 ggttttgatg aaacttatac agagacgaaa aaaaagtaag tgaggcgctc aagtcacctt
    21901 catttaagta ttcgattact taccgagaat tagatataaa gacgcaatta ctcgaagcag
    21961 gttggacgtt accagaaatt gaacgtactg acttggatga gttaatgcgt ctttttgctt
    22021 ttagagatgc ggtaaaagag catgaagatg ttgagtacta tgataatttc actcaatttt
    22081 aggaggtgat actttgaata acgatgacct aattctgaag atgatactag atgaatctgg
    22141 ttttactgcc ggtatgaata atgctgtcaa aaagttgaac tcttttgatg aaacaatcga
    22201 aagaaaaagt agaaatagtg gcagttcatt aggtagtatt tggaaaatat ttgctggaag
    22261 ttttctagct agcggagtaa ctagaatcgt aggagctggc tttgatctaa tcaagggttc
    22321 cataagtgga gcggttgatc gagtagatac gatgaataac gctctacgaa atttccaaaa
    22381 tatgggattt agtaattcag aaattatgaa gaatatcgga aagaatgggc ttttatctca
    22441 aggtattcaa ggacttccta ctgctttgaa tgatgcgata agtcatgttc aacttcttgc
    22501 ttcttctaca ggtgatttaa ctcgttcaac tcaaatattt aaagctttaa atgatggaat
    22561 tcttggtttc ggtggttcaa ctgatcaagt taatgaagct gttattcaat tatctcaaag
    22621 tttctcaaac ggaaaagtag acgcacaaac ttggaactca atgatcaacg ctcaacttgg
    22681 acctactctt tctgctattg ctaaaaagat gggaattaca atgggagatc tgaaagaagg
    22741 tttatctcaa ggtaagattt ctgttgaaga attccaaaat caattaatag aaatggatac
    22801 caaaggtggc ggaggactta aatcattaag tcaaatcgct aaagattcta ctaagggaat
    22861 taaaacctct atacaaaatg caaaaacagc tgttacacgt ggtgttggtg aggtaataga
    22921 aggattaaat aaagcattag tagattcaga tttaggtgga tttaagggaa ttattgataa
    22981 agtgggcagt tcaatggaat cgttcttaaa agtaattgct gcaaatattc ctacagcagt
    23041 atcattttta agtaatttat ttaacgaaat tcaaaaattt ggttctgctt tgaaattcat
    23101 gatgcccttt cttgttcctg cagctaccgc ttttggagcg ttcatgtttc aacttaaagg
    23161 tataccagca ataataaaaa gcttcaataa ttttaagaat gccataattg gcgttggaaa
    23221 ttcattaaag attatggggg caatagctgc tgctaatcca tttgttttaa ttattggtgc
    23281 agtcgttggt gcaatagctg ttttcggtta ctttatggca accaatgaag agcttagaaa
    23341 caaggttata tctatttgga atgatgtgaa agattccgta attggtgtat taaaaaatat
    23401 aaaggactgg ggaattgata cttggaattc tgctaaagaa atggcatcaa atgcagttga
    23461 gggtgtcaaa gatgcttggt caggaacaaa ggaatggttt tctaacacct ggaaaggtat
    23521 taaggatggg gccacaggtt tatttgataa aacagtagag acttcaagaa atgcagtcga
    23581 tagtgtaaaa aatgcatggt ctaatatgaa gaaatggttt tctgatactt ggcaaagtat
    23641 aaaagattca gccagagaaa aatggaatgg aatcaaaggt tctattatgt cagttgctgg
    23701 tccattgatt acaggtataa agaatgcatt tttacatgtt actttttatc tagagacctt
    23761 atggaataat ttagttgaga ttggtaaaaa cgtctttgag attttaaaaa atgttatact
    23821 tgcgccggtt ttgtttatta cctccatgat ttctggtgga tgggaagaaa caaaaaataa
    23881 tatgattgct gtttggaata atattaaaga aagtgccata aatatttggg aatctataaa
    23941 gaatatattt gtaagttatt ttacaaatat ttattttgct gcactcaata tttggacagg
    24001 gttcaagctt acattgataa atatttggaa tgaagtggta aatcaagcta aatcgatttg
    24061 gttaaacata aagtattttt ttattaatct ttggattgat ataaaatatt ttgcaattca
    24121 gaaatggatt gaattgaagt tttctattat tcaaacttgg atcgatttaa aatataatgc
    24181 cattactact tggaataata ttaaacagtt tttcaaagat acttggaaaa atattaaaga
    24241 tacggcatac aatacgtgga tttctataaa aaataccatg attaatacat ggaataacat
    24301 taaggactct ttctggaata ttgttactgg aattgttaat tctgctgaaa atgcatggac
    24361 aaatctaaaa aatggtgttt caaatgctat taatcgggta aaagaaatct ttaattcgtt
    24421 aagggaagtc aatttatttg aaattggcaa gaacatcatt gatggactta taaatggtgt
    24481 aaaagaaaag tggaatgctt tgaaagatac tatcaaaggt attgcaggta atatcaaaaa
    24541 ttggattaaa ggagctttag atattcattc tccatcaaga tggatgcgtg atatggttgg
    24601 taaaaatatt gttcaaggta ttatcgttgg tattgataaa gagcaaagta agttagatca
    24661 aaccatgaca aatttagtaa aaacaccgtc tgttcaacct gtagtaaatg gaataagtca
    24721 cctaccaatt acacaaacaa agcaaaatat atctgctagt gaaacaaaag aaattcattt
    24781 acatttaaat gtttatggag atttacctga ttcaatggtt cggaagatta ccacaaaaat
    24841 taaaacagaa ttgacaagac aaatgaaacg agatgctggt gcagtaggag gaacattata
    24901 tgcaacttaa gagaggacaa ttttttatta atcaacatta ttcttctgaa tttaatgtgt
    24961 atattcaaaa caggcctgct tctgtttcag ctagtcgagt aattgaattg agagagcgag
    25021 aaggaaatga ttctatcatt attgataaag gctattacaa aaatgtgact agaaagattg
    25081 agtgttacta taaagcacct tcaattgata tggtgcagga gtgggaagat cgaatcactg
    25141 aatggttaga tatgtcgtct tatagtgatt ttattcttta ctatgatgaa caatatattt
    25201 atcaagctgt agtaatagaa ggacctgaat ttaaaggaac aagaaaaaca ggaaatattg
    25261 tcccttttga atttacagtg agtattcgac catttaagga aaattacaat ggtagattta
    25321 ctattcgaca aacagaaact tttgaaattt ataacccaga gaagtattct tcaaaaccgc
    25381 ttattaaatt gagtggttct ggagatgctt ctttttatat taataaggat aaatatgatt
    25441 tgaaatcgtt ggacagagaa ttatttatag attctaaatt agaagaggct tatcgaaaat
    25501 tagatggtaa tttagaacat caggatcagg tcaccttatt tttagatttt ccatttttat
    25561 atcctggaaa aaatgaaatt aaatggacga aaaatattca ttcatttgaa ataatgccta
    25621 ggtggtggag aaaagtatga aaccaagaat atatagtcct actgaaacag atttctcaac
    25681 gaatggctta ggaattttaa aggattgtac aagatgtgga atatatgaag tagctaatgg
    25741 aaaatatgaa ttagagttgg attatccttt aggaactaga tttgatgaat attttgaaaa
    25801 tgactatcaa ataaaagcaa agccaaacga tcaagaagag tatcatatct tttttattga
    25861 tgataaagat atagatactt ttttaaatac agtaactatt tatgctcaga gtcgtacaaa
    25921 tcgacttgga agacgggtag tcactcttgt ggaagtggac tctaaaactg gtcaagaagc
    25981 gatatcaatt attgaaacta aaatggataa aaaatctgac atacgacttt attctgatat
    26041 tacggctgtt tctagcacaa tctttgaagc gagaaatgtt ttaaattgta ttgctggtga
    26101 acaaggatca ttgcttcaat attggggcgg agaaattaaa cgtgaaccat ttaagctttc
    26161 tttgttaaag cgaagaggtc gcaataatgt tggaacgatt cggtatggaa aagatatgtc
    26221 tggtttgaag gtcaaattag attggacagg tataaaaaca agaattattc catatgctga
    26281 tcctcaaagt gaagtaggta cgactaatcg aatttatggt tcgccagtag atagtgccta
    26341 tattaataac tatcctgatg tctatacaga acatgttcag tttacagaag aacaaggagt
    26401 aaaagacatt aagagcttaa ataaaatagc taaaaattac tttaaaacga tcaatcctgg
    26461 ctgtgataag cctaaagttt ctattacggt tgaatttgat aagttgactg atagtgaaga
    26521 agcgaaagaa tttgcgaaga ttagaaatta tggtctgttt gatacattta aaatatatca
    26581 taaaaaatat aatatttatc ttgaatcaaa ggttagtgga ctacaatacg attctttatc
    26641 tgaaaaaact ttgaaattag aagcaggaga cattcaagtt gctttttatc agcaacaagc
    26701 tgtaactatt caggataaat taaaagatta tgcaacgaat aattatatga gtgattttaa
    26761 tgattatgtt tcttcaatga ttacaggtca aggcagtgca ggaggatatg tagttttatg
    26821 gccaaaagaa aaaccttcca acatctttat tatggataat ccagatttag aaaaggcaag
    26881 agaagtacta agaatgaata aaaatgggat tgctttttct aagaaaggat ggcgggggcc
    26941 ttttaattca gcttggacat tagatagtat atttaatgcc aattttatac aaactggatt
    27001 aatcaaagca gatatctttc aaaattcttt taacaagact ggagatgtat tgaaattagt
    27061 taatggacta ctccaaattt ggaacaacaa aaagaaaata atggagttaa ctaaaaaagg
    27121 aatggaattt tggaattcta agggttcaat tggaacgatt ggaacaactg attctgttgg
    27181 taatcctttt cctggggctt ctactccaac ccctattgaa gataattctt tagttattcg
    27241 tacaaatgga gacggcaaat atattttgat ttctcctaaa gtaggaaaag gattagtttt
    27301 attaggaaat ggtaaagcaa tttattttgg agacttagat gtacaaggaa aacttacagt
    27361 taaaggaaaa gaaataattc ctggacaagg tggtggtcca tcgggaggtg gagaatctac
    27421 tggtgggtat cctagtgaat taaaaacaga tgcagaaaag agagcttgga gaat
//
LOCUS       DAZKOU010000031        27289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000031 DAZKOU010000000
VERSION     DAZKOU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.031"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            296..1465
                     /locus_tag="ACG827_001273"
     CDS             296..1465
                     /locus_tag="ACG827_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0483427.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1938..2492
                     /locus_tag="ACG827_001274"
     CDS             1938..2492
                     /locus_tag="ACG827_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0483428.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2665..3138)
                     /locus_tag="ACG827_001275"
     CDS             complement(2665..3138)
                     /locus_tag="ACG827_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0483429.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3150..4097
                     /locus_tag="ACG827_001276"
                     /pseudo
     CDS             3150..4097
                     /locus_tag="ACG827_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4368..4803
                     /locus_tag="ACG827_001277"
                     /pseudo
     CDS             4368..4803
                     /locus_tag="ACG827_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4819..5652
                     /locus_tag="ACG827_001278"
     CDS             4819..5652
                     /locus_tag="ACG827_001278"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0483430.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5897..6922
                     /locus_tag="ACG827_001279"
     CDS             5897..6922
                     /locus_tag="ACG827_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0483431.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7060..7185
                     /locus_tag="ACG827_001280"
     CDS             7060..7185
                     /locus_tag="ACG827_001280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483432.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7210..8325
                     /locus_tag="ACG827_001281"
                     /pseudo
     CDS             7210..8325
                     /locus_tag="ACG827_001281"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8408..9304
                     /locus_tag="ACG827_001282"
                     /pseudo
     CDS             8408..9304
                     /locus_tag="ACG827_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9320..10144
                     /locus_tag="ACG827_001283"
     CDS             9320..10144
                     /locus_tag="ACG827_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0483433.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10176..11486
                     /locus_tag="ACG827_001284"
     CDS             10176..11486
                     /locus_tag="ACG827_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFQ0483434.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11634..12893
                     /locus_tag="ACG827_001285"
     CDS             11634..12893
                     /locus_tag="ACG827_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0483435.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12903..13775
                     /locus_tag="ACG827_001286"
     CDS             12903..13775
                     /locus_tag="ACG827_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0483436.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13787..14617
                     /locus_tag="ACG827_001287"
     CDS             13787..14617
                     /locus_tag="ACG827_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0483437.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14657..16840
                     /locus_tag="ACG827_001288"
     CDS             14657..16840
                     /locus_tag="ACG827_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0483438.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16901..18121
                     /locus_tag="ACG827_001289"
     CDS             16901..18121
                     /locus_tag="ACG827_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0483439.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18118..19578
                     /gene="gtfA"
                     /locus_tag="ACG827_001290"
     CDS             18118..19578
                     /gene="gtfA"
                     /locus_tag="ACG827_001290"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFQ0483440.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19655..20761
                     /locus_tag="ACG827_001291"
                     /pseudo
     CDS             19655..20761
                     /locus_tag="ACG827_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21083..21292
                     /locus_tag="ACG827_001292"
     CDS             21083..21292
                     /locus_tag="ACG827_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0483441.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21285..21434
                     /locus_tag="ACG827_001293"
     CDS             21285..21434
                     /locus_tag="ACG827_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0483442.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21588..22253
                     /locus_tag="ACG827_001294"
     CDS             21588..22253
                     /locus_tag="ACG827_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0483443.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22537..23622)
                     /gene="xerS"
                     /locus_tag="ACG827_001295"
     CDS             complement(22537..23622)
                     /gene="xerS"
                     /locus_tag="ACG827_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0483444.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23956..24189
                     /locus_tag="ACG827_001296"
     CDS             23956..24189
                     /locus_tag="ACG827_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFQ0483445.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24186..24593
                     /locus_tag="ACG827_001297"
     CDS             24186..24593
                     /locus_tag="ACG827_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0483446.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(25730..26248)
                     /locus_tag="ACG827_001298"
     CDS             complement(25730..26248)
                     /locus_tag="ACG827_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0483447.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            26615..26863
                     /locus_tag="ACG827_001299"
     CDS             26615..26863
                     /locus_tag="ACG827_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483448.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9336 a         4143 c         5037 g         8773 t
ORIGIN      
        1 aaattaataa tatactacca tttgttggta gtataattta aaggtaataa gttattcttt
       61 tattactatg tttgggtttt atagcaaaga aagtttaata agagtttgga cctagcaaac
      121 aatattaatg aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag
      181 acaaaatgat agcgtaaaat attttgtgta aatagatatt tagggtagaa aaaaagaaag
      241 tccattctgt aaaattaaag ttaccacaac cgaaatttta taggaggact ttctcatgcc
      301 caattttact acagaaatta tggaaacact aatcaataaa ggtgatttag atgaattatt
      361 tcgatgtcat ttagaattag ctgttaattc attattgcag gctgaattaa cagcttttct
      421 tgattatgaa aaatatgatc gtgctgggtt caattcaggt aattcccgta atgggaatta
      481 ttcacgttca ttcagaacag agtatggaga attaaatttg gtaattccta gagatagaaa
      541 tggtaagttt tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac
      601 cactattatt cagctatttc aaaaaggaat tactatgtct gaaatctctg aattgattga
      661 aaaaatgtac gggcatcact atacgccaca aaccatttca aatatgacta aaatagtttc
      721 tgaagatatt attgccttta aagaaaggtc cttagaatcc agatactctg tcatttttat
      781 ggatgctact cacattcctt taaaaagaca aactgtttca aaagaagcag tttatattgt
      841 aattggcatt cgattagatg gaaccaaaga ggttcttggg tttagtcttg ctccaactga
      901 atctgcttat gtttggaaag agattcttca agacctaaaa gaccgtggtt taaaagaggt
      961 tttattagtc gtaactgatg gtttaagtgg cattaacgat agtattcata gtgtttatcc
     1021 aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg
     1081 tgttagtgat cgacaagaaa tctgtagcga tttcaaatta gtttatcaag caagttcaaa
     1141 agaagaagca aataatcaaa taaggtttat gatagataaa tggaaaaagc agtatccacg
     1201 agtggttaaa ttgctcatga atcctgctat attaaccttt tataatttcc ctccgtcaat
     1261 cagaagaact atttattcaa ctaatttgat tgaaggtttt aacaaacaat taaagaaata
     1321 taccaaaaga aaagagcaat ttcctaatga agaatctctg gagagatttc tagtatctca
     1381 attcaacaac tataatcaaa agtttctatg ccgagttcat aaaggtttta aagaaataca
     1441 tgatacatta gaatcaatgt tttaacttga tcaacagaat gggcttttcc atttacacaa
     1501 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
     1561 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
     1621 atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt
     1681 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct
     1741 tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt
     1801 tactgaaatg atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt
     1861 atatatataa gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt
     1921 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
     1981 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
     2041 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
     2101 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
     2161 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
     2221 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
     2281 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
     2341 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
     2401 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
     2461 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
     2521 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
     2581 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
     2641 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
     2701 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
     2761 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
     2821 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
     2881 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     2941 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     3001 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     3061 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     3121 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     3181 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     3241 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     3301 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     3361 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     3421 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     3481 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     3541 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     3601 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     3661 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     3721 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     3781 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     3841 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     3901 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     3961 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     4021 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     4081 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     4141 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     4201 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     4261 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     4321 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     4381 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     4441 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     4501 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     4561 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     4621 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     4681 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     4741 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     4801 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     4861 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     4921 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     4981 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     5041 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     5101 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     5161 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     5221 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     5281 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     5341 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     5401 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     5461 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     5521 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     5581 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     5641 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     5701 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     5761 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     5821 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     5881 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     5941 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     6001 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     6061 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     6121 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     6181 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     6241 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     6301 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     6361 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     6421 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     6481 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     6541 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     6601 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     6661 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     6721 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     6781 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     6841 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     6901 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt
     6961 ttactaatat ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt
     7021 taacaaaaag aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata
     7081 gctttaacag ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat
     7141 agtaattcta aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct
     7201 ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa
     7261 atggagacaa cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt
     7321 aatatgccag atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat
     7381 gctttgatgg gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact
     7441 gaaggaattg ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct
     7501 tctgtagtgg ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca
     7561 gatgaaacat ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca
     7621 aaaggcatct atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt
     7681 tatcaaaatg ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca
     7741 gaagcacaag aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca
     7801 acaattacag aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca
     7861 atgatgattg aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa
     7921 aatgttgctt ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt
     7981 ggttgggctg ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt
     8041 ttagctagtg aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta
     8101 aaaggctttg aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga
     8161 aaaatgttgg agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa
     8221 gcagaagttc aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc
     8281 gctcaaattg aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac
     8341 aatatacagg cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg
     8401 ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta
     8461 tatttttatt tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca
     8521 aacagcaatt ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt
     8581 aagaaattac caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt
     8641 aatttatgca gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt
     8701 aatgaataga aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt
     8761 tgctgcacca tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact
     8821 gattaataac atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc
     8881 gattgcgatt tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt
     8941 gttattacta gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga
     9001 tggtgctagt ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt
     9061 attttttgta gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat
     9121 gatgattact cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta
     9181 taaacattca tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt
     9241 agtgattata ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta
     9301 ttaaggaggc tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat
     9361 gcttatttta ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg
     9421 gaaaacgttg gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct
     9481 aagtaactat caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt
     9541 tttaatggtt gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc
     9601 ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat
     9661 gatgattccc ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat
     9721 tttaaataca attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt
     9781 attaagacaa tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg
     9841 tgccaatatt tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt
     9901 tgcacttgga atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt
     9961 caatacttca attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata
    10021 tgccacgaac ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt
    10081 gatattcatc gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt
    10141 ataaaactat taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg
    10201 caggaagtac tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac
    10261 aagatgcaca tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa
    10321 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
    10381 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
    10441 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
    10501 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
    10561 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
    10621 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
    10681 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
    10741 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
    10801 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
    10861 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
    10921 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
    10981 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
    11041 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
    11101 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
    11161 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
    11221 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
    11281 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
    11341 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
    11401 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
    11461 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
    11521 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
    11581 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
    11641 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
    11701 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
    11761 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
    11821 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
    11881 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
    11941 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
    12001 gatatgcaca acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg
    12061 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
    12121 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
    12181 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
    12241 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
    12301 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
    12361 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
    12421 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
    12481 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
    12541 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
    12601 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
    12661 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
    12721 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
    12781 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
    12841 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
    12901 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
    12961 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
    13021 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
    13081 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
    13141 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
    13201 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
    13261 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
    13321 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
    13381 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
    13441 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
    13501 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
    13561 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
    13621 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
    13681 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
    13741 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
    13801 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
    13861 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
    13921 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
    13981 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
    14041 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
    14101 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
    14161 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
    14221 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
    14281 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
    14341 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
    14401 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
    14461 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
    14521 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
    14581 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
    14641 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
    14701 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
    14761 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
    14821 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
    14881 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
    14941 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
    15001 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
    15061 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
    15121 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
    15181 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
    15241 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
    15301 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
    15361 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
    15421 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
    15481 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
    15541 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
    15601 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
    15661 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
    15721 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
    15781 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
    15841 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
    15901 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
    15961 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
    16021 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
    16081 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
    16141 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
    16201 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
    16261 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
    16321 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
    16381 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
    16441 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
    16501 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
    16561 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
    16621 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
    16681 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
    16741 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
    16801 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
    16861 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
    16921 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
    16981 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
    17041 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
    17101 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
    17161 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
    17221 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
    17281 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
    17341 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
    17401 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
    17461 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
    17521 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
    17581 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
    17641 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
    17701 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
    17761 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
    17821 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
    17881 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
    17941 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
    18001 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
    18061 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
    18121 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
    18181 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
    18241 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
    18301 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
    18361 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
    18421 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
    18481 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
    18541 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
    18601 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
    18661 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
    18721 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
    18781 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
    18841 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
    18901 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
    18961 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
    19021 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
    19081 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
    19141 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
    19201 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
    19261 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
    19321 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
    19381 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
    19441 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
    19501 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
    19561 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
    19621 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
    19681 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
    19741 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
    19801 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
    19861 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
    19921 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
    19981 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
    20041 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
    20101 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
    20161 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
    20221 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
    20281 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    20341 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    20401 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    20461 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    20521 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    20581 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    20641 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    20701 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    20761 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    20821 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    20881 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    20941 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    21001 aaatttgaat aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt
    21061 gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg
    21121 ttatgggaag tatcagtctg agagaatcat acgacaggca aaagcaatta tggtttcaaa
    21181 aagctatacc tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga
    21241 agttttaggt atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa
    21301 aagataaaaa tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga
    21361 aaagagtaca aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc
    21421 gaacttgttt ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag
    21481 gagttttttt ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat
    21541 aaaaatcggt tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag
    21601 aaatacgtcc aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt
    21661 cttatgtaag aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg
    21721 gactacttga aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag
    21781 ttgatttttg gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt
    21841 tgtaccaaca ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg
    21901 gagaagccac tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga
    21961 ataaaacact ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt
    22021 ctagtattcg tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg
    22081 agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc
    22141 agaaatatac aatccgtcat attatgagaa gacatgcaac gatcgttggt gttcatataa
    22201 tccgcataca cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg
    22261 cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg
    22321 gtcatttgta tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa
    22381 ctaatttttc taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca
    22441 aaagtaggat cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg
    22501 tgttgtttct tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat
    22561 cgtagccgca ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa
    22621 ttgttgggca gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg
    22681 acgcagtttg tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg
    22741 ttcgattgca ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc
    22801 tttttcgttt tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata
    22861 ttccattgcg aatgctgcta tattgacagc atctctttta tttccttttc tagttactac
    22921 cacattattc ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct
    22981 caatccagat cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata
    23041 atagatcaaa gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata
    23101 attttgtgta tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa
    23161 cgtagtttta tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt
    23221 tcctagagga ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat
    23281 atttctattg atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt
    23341 tgttgtctct ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt
    23401 taggtcatag gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc
    23461 actcagaagc cattcataaa agagcttata attttttaga tattctaaag tggttcgagg
    23521 tgattgatct ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag
    23581 tatgattgta tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat
    23641 aagtagggga gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa
    23701 ttggtatgct ctaaaattta ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt
    23761 taataattta aaataaatgg tatacataaa tagggaagga aatagaaggg agcaatctaa
    23821 attttttgaa ttttcttaat taattatata gaaagaacaa tctttctata gagttagaca
    23881 tgagagggac tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa
    23941 aggagggaga agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta
    24001 gtattaacta tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt
    24061 tttttagatg aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa
    24121 attgatttag caatatctca agcattaact gaatatgata ctacttttag agagcttgtc
    24181 gataaatgag tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa
    24241 taaaaaaata ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt
    24301 gtgtagggca acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa
    24361 aagcagctat cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac
    24421 gtactgcttt cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg
    24481 atcaaaaaga tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc
    24541 aactaaaata tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta
    24601 ctgtatgtgt ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa
    24661 atagctataa ttcaaatcca actatataga aaacgtaaat tttctatata gttggattaa
    24721 gataacgact atttatttta tcatattttt actataccct atacttttac ttggtatatt
    24781 tatgaataat cgcaaattta cccctatact actagtttgt aagttagatt aataaaaata
    24841 ctaaagttat ttgttctttt attaaataca cctacaactt ataaattgat ggtatttaga
    24901 taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt gctactcaat
    24961 cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga aagacctttt
    25021 acatcttgca gaaatcgtta ctgggattaa actaagcaat aaaaactaat gttcccctca
    25081 ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt caccttcagg
    25141 atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa taggataggc
    25201 gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta tcgtatgttg
    25261 gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt gaatcgttct
    25321 taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga cttgccttac
    25381 ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact atcggcggtc
    25441 tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag tccctttgta
    25501 gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc tgttggccaa
    25561 ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag tgcacttgca
    25621 ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt tcttactgtt
    25681 aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt tacaaagcta
    25741 atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact gtatttacat
    25801 gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc cattttcttt
    25861 tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta atatcatcaa
    25921 tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag gtatttgtaa
    25981 accacttata taactctcct aactgattcc atttaaattg atcagaaggc gttttaacag
    26041 ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa ccaacttgat
    26101 atgctaaatt ttcagcaggt gttctatcca cttcctcaac tcttttatcc ttcaattctt
    26161 caggaatagt ttcaaattct aaaatatatt tagtataagt cctatttatt tcttttttta
    26221 attcttcttt atttttatat gttctcatta tattacctcc ataaattcct aattgtctcc
    26281 tttaattcat ctataatttc atccacgata ttttaatccg ttctacaatg catatctgat
    26341 atgcattgac tgtctaactc atatgtgaat gatttataaa atatcccaac gtctcatcta
    26401 aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa
    26461 atcggactgg tttgcatatc attttcttcg taaatatcaa aatcatgtta actactatca
    26521 attactaata aaaactacca ctttaagcgt taagaactgt taagtttagt taagtgagga
    26581 tagttcacaa aatccattgt taaacttcac gaaagtggta aaactcaaaa tgctttatat
    26641 aggattacag catcgctctt tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa
    26701 aattgacgat gacacgttcc ttacggctca tcagatgaaa cagctacaaa aacgaaatga
    26761 tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg
    26821 aactattaaa ggtcaagatt tacctgaaga tcatcctttt taggaaattg aagcttttta
    26881 tgttgcacaa tgcatttata atactacaat gatgttttca ccagatattc ttctagggtg
    26941 cggtgtaatg aaacagatac acttgcaaaa gaaaattcac cgcgaattta ctaaaattat
    27001 gaataattat gttaatcttc cgaaattttt ttccaaaatt aagggatcaa gctggtgtta
    27061 tagattgttt aaggttggca aaaaaataac ttctttttaa acaattatag aaaaccgata
    27121 ctctgttaag ttggttgaca cggtgtcgat ttttatgact ttcttattct gaaacttcac
    27181 taacggacag tacctaaaaa gtctatagta tggacgttta cgagtactat tttatcaaaa
    27241 atgctgctca tttcaactta tatgttttag tgaactaccc ccacttagc
//
LOCUS       DAZKOU010000032        26708 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000032 DAZKOU010000000
VERSION     DAZKOU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.032"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            350..2044
                     /locus_tag="ACG827_001300"
     CDS             350..2044
                     /locus_tag="ACG827_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0483449.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            2732..4609
                     /gene="ltrA"
                     /locus_tag="ACG827_001301"
     CDS             2732..4609
                     /gene="ltrA"
                     /locus_tag="ACG827_001301"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0483450.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            4740..5117
                     /locus_tag="ACG827_001302"
                     /pseudo
     CDS             4740..5117
                     /locus_tag="ACG827_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            5117..6322
                     /locus_tag="ACG827_001303"
     CDS             5117..6322
                     /locus_tag="ACG827_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0483451.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            6338..6982
                     /locus_tag="ACG827_001304"
     CDS             6338..6982
                     /locus_tag="ACG827_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483452.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            6993..7799
                     /locus_tag="ACG827_001305"
     CDS             6993..7799
                     /locus_tag="ACG827_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483453.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            7821..8051
                     /locus_tag="ACG827_001306"
     CDS             7821..8051
                     /locus_tag="ACG827_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483454.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            8597..8872
                     /locus_tag="ACG827_001307"
     CDS             8597..8872
                     /locus_tag="ACG827_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483455.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            8914..9336
                     /locus_tag="ACG827_001308"
     CDS             8914..9336
                     /locus_tag="ACG827_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483456.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            9355..10947
                     /gene="mobP2"
                     /locus_tag="ACG827_001309"
     CDS             9355..10947
                     /gene="mobP2"
                     /locus_tag="ACG827_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0483457.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            10959..11294
                     /locus_tag="ACG827_001310"
     CDS             10959..11294
                     /locus_tag="ACG827_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483458.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            11441..11635
                     /locus_tag="ACG827_001311"
     CDS             11441..11635
                     /locus_tag="ACG827_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483459.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            11667..14039
                     /locus_tag="ACG827_001312"
     CDS             11667..14039
                     /locus_tag="ACG827_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0483460.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            14100..15560
                     /locus_tag="ACG827_001313"
     CDS             14100..15560
                     /locus_tag="ACG827_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0483461.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            15571..17775
                     /gene="topB"
                     /locus_tag="ACG827_001314"
     CDS             15571..17775
                     /gene="topB"
                     /locus_tag="ACG827_001314"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0483462.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            17791..17940
                     /locus_tag="ACG827_001315"
     CDS             17791..17940
                     /locus_tag="ACG827_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483463.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            18014..18607
                     /locus_tag="ACG827_001316"
     CDS             18014..18607
                     /locus_tag="ACG827_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0483464.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            18623..19522
                     /locus_tag="ACG827_001317"
     CDS             18623..19522
                     /locus_tag="ACG827_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0483465.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            19525..20010
                     /gene="ssb"
                     /locus_tag="ACG827_001318"
     CDS             19525..20010
                     /gene="ssb"
                     /locus_tag="ACG827_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0483466.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            20376..20636
                     /locus_tag="ACG827_001319"
     CDS             20376..20636
                     /locus_tag="ACG827_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483467.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            21077..21283
                     /locus_tag="ACG827_001320"
     CDS             21077..21283
                     /locus_tag="ACG827_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0483468.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            21283..21534
                     /locus_tag="ACG827_001321"
     CDS             21283..21534
                     /locus_tag="ACG827_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483469.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            21549..21986
                     /locus_tag="ACG827_001322"
     CDS             21549..21986
                     /locus_tag="ACG827_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483470.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            21979..22686
                     /locus_tag="ACG827_001323"
     CDS             21979..22686
                     /locus_tag="ACG827_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483471.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            22851..23051
                     /locus_tag="ACG827_001324"
     CDS             22851..23051
                     /locus_tag="ACG827_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483472.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            23067..23246
                     /locus_tag="ACG827_001325"
     CDS             23067..23246
                     /locus_tag="ACG827_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483473.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            23587..23850
                     /locus_tag="ACG827_001326"
     CDS             23587..23850
                     /locus_tag="ACG827_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0483474.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            23844..24194
                     /locus_tag="ACG827_001327"
     CDS             23844..24194
                     /locus_tag="ACG827_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0483475.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(24218..24832)
                     /locus_tag="ACG827_001328"
     CDS             complement(24218..24832)
                     /locus_tag="ACG827_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0483476.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            25146..25568
                     /locus_tag="ACG827_001329"
     CDS             25146..25568
                     /locus_tag="ACG827_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483477.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            25578..25781
                     /locus_tag="ACG827_001330"
     CDS             25578..25781
                     /locus_tag="ACG827_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483478.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            25992..26576
                     /locus_tag="ACG827_001331"
     CDS             25992..26576
                     /locus_tag="ACG827_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0483479.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         9923 a         4271 c         5480 g         7034 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaaccactc tacaaaaaag aattgatcaa gattctgtat cgaatgaaca atatgaatga
      181 tgagtaggaa tggagaagag agaatgaaat atccgttgat atggttagaa aaagatgttc
      241 cgaaggaaga gaccactaga aaatttcctc acactttaag cctgggtaag ttgggaaaag
      301 ggcaatcttt taaccaagta aagaatcaaa caagaagagg tgactgtaca tgttagaagc
      361 aattaaaaca gctttttcac cacaaaatga tcaaaaagta cttgatccat taaagcaact
      421 aggaaatcgg gaaatagaaa ttgtaaaatc caaagggtat gatttagatt ttttaaaaca
      481 agtgatgcca caaggtgggt taaattttga tgaagaatat gtgacgtatg gtaatggtta
      541 ttcacgatgt gtgacagttt atcactacac aaaaaatcct acgctttttt ggttggccaa
      601 tctcatgaac aatcctcata cagtggccac actcgatgtt agcacagatg aaaagcaaaa
      661 agtgattgat caaatcaatc gtgccttaga tgaattaaat gatcgatcag ataatgaacg
      721 aaaagcgact gaccgaaata aaagcgcgga agaatacctg gaactagcgg aatatgctag
      781 aaaattgact tctaatgggg aaatttctaa gcaagtcaaa gtgagagtac atgtttacgg
      841 aaacacgatt caagaagtag atgaacgagc gcaggaactg atcgaaaatc tcaaagggat
      901 ggattatcgt gcggtgactt atctgcataa aaccaaacga gattggcaat cgatgtttac
      961 catgttcgat gaacaagctg aatggttcgg aaatatcaaa gggcaatcac taccatcgtt
     1021 aaacattggc ggaggtttcc cgttccacca ccaaacgcta aaagatccag gagggacaca
     1081 atatggggaa accatgacag gcggcccatt tgtctggaat gcgacgtata aaaatagtgt
     1141 acggttgtct actaatacct tgcttctagg aatgatggga gctggtaaat cgaccgttct
     1201 taaaatgatc gcggaagccc atttagctgc aggagacttt atttggggct ttgaaaaggg
     1261 aaaagacttt attcctttct taaaggaata taacggtatt atggtgcgtt tagacggatc
     1321 tgacggaatg atcaatcctt tagagatatt tgccacacgg acgtatgatg aagcatccag
     1381 tttatatgac gatggatcag tcaaagattt gaagatcaat gaagcagcgt cctatcaaac
     1441 acacttagac aaagtggtct atcaggtaca gttagtgagt cctcagctaa aaggaacgat
     1501 gaaagcagaa tttaagacct acttaaatcg tttttatgaa gagtatggaa cagtaccacg
     1561 cggctttact tcctcaaatt cgcgttcaaa tactgatacg caagtcacag ggaaagatcc
     1621 agaagcttat ccgacgttca aagaattttt agacttttta ggacaactgg aattaccagg
     1681 tgcttcccaa gagaaaaaga atcgaaaaga agaaatggaa tccatcgtag aatccttatg
     1741 tgagacgtat ggcatgattt ttgatggtca tagtacgatt cgacaccttg accaacaaca
     1801 gttggtcttt tttgacattg actctattac aggcttagat cgaggggtac aacaatgtca
     1861 aatgtacatg gctatgacgt tgatttggaa tcaggcattg attcaaggaa gaagacaacg
     1921 acgtttaatt aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat
     1981 tgatgagtgc cataatggtg cgacctgttc tgcagtgaaa agctgtgggt tgcaagtagt
     2041 ctaaaaacta ttgtaataac tcttataacg agaagtcaaa tgagtgggta acgccacaaa
     2101 gggcttccac tgatactccg acatgcaaca agtagtttat gcagaccaac gttgtgtgaa
     2161 gctcggtgaa gtcggctgaa ggataccgta aggtaaaaga aatagtgaat accacgaaag
     2221 ttctctagag gtaaagggtg tatccgataa gtcgggtgtc caaaaaaact taatatggtg
     2281 agtatgtaca aacgtactga cgaactagcg aatgtactgt ccgaaaggca tagctcctag
     2341 aaatgggaga gacagtgagc aaaaaatcac tcattgtgct aacttgtggt tgagtaaaag
     2401 tcgttgttat gaaagaccat gctgcgttac aggcgtagga gaagttaggg ggcaaaagct
     2461 agcatctaag ttaaggcatg tggatagata taagggaaca ggggaagcca tcaacatgga
     2521 gagtgcatac tcgatgaagt ggtagtaaga aatatgtgaa tgacaaataa cttactttag
     2581 tgatggtgaa agtagagtca cgactgatga aggttctgta atggaactgg aggaacaggc
     2641 tcgattcgtc tgtaaaagtc atgaaacccg ttcaaccatg ggattctcgt atgactaaaa
     2701 aggtcacgag ccaattggag cgtgataaac catgacaaaa gaaacaagtc caaaagatag
     2761 gaagttgcgg catgcagaat attatggcat gacggaaatc ttcgataaga tatactccaa
     2821 aagtttgaag ggagataatt tcaagaatct aatgcagatt atcatttctg ataacaacat
     2881 tgtcctagca tatcgtaata tcaagagaaa tcacggtagt gtgacaagag gagaagatgg
     2941 agtaacgatt aaagatattg aaaatctcaa ccgtaatgaa ttcgtagaaa ttgttaggaa
     3001 gcgattcgca cattataacc ctcggaaagt aaggcgtgta gaaattccta aatataatgg
     3061 taaattaaga ccgttgggta ttccatcaat atgggataga atcgcgcagc aatgcatctt
     3121 acaagtatta gaaccaatat gtgaagcaaa attcaataat cacagctatg gtttccgccc
     3181 aaatagatca gcagaacacg caatagcaga ttgtatggcc cgaatgaatc aaagtcacat
     3241 ggaatatgtg gtagatgtag atatcaaagg tttcttcgac gaagtcaatc atactaaact
     3301 catgagacaa atctggtcta tgggtatacg agacaaacag cttctcttaa tcattaggaa
     3361 aatgttaaga gcacccgtag tacttccaaa tgggaagttg atatacccaa caaaaggaac
     3421 accacaaggc ggaatactta gcccattact tgcgaacatc aatctcaacg aatttgactg
     3481 gtggatagca aatcagtggg aggaaaaaga tgccaaagag ctaaaacatc attttaggaa
     3541 aaatggagct tgggataaag gtaatatcta tcacaagctc agaaagagta caactctaaa
     3601 agaaatatac ctagtgagat acgcagatga ttttaaaatt ttctgtagaa attataatga
     3661 agcgaaaaaa gttttctatg caactaaaat gtggttacag gagcgtctaa agttaccaat
     3721 atctgaagaa aagtcaaaaa tcacaaatct ttcaaaagaa gaaagtgaat ttttaggctt
     3781 tacgatgaaa ttagtgacca aaggtaacaa aaaggttgtt aatacgcata taggtacaaa
     3841 agcacaaagc atgattaaag tgaaactaaa aaaacaaatc aaacgaattc aaaaaagtcc
     3901 taatagcaaa gataccttta tctatattgg agtctataac agtatggtta ttgggattca
     3961 taattacttc aaaattgcga ctcattgtag tgtagcaatg cgaaaaattg caagagaact
     4021 cagaccaagt tttgaaatac gactacgaga acaaggaatc tcaaaagaag gacaatacgg
     4081 aaagtacgaa ggatacaagc cttacctacg gtcaggacaa atacgatata taagagggtt
     4141 tccaatatta ccagtagggt atattcaaca cagacatccg atgtgtaaaa aaagaaatat
     4201 taataaatac accgaagaag gtagggaagc cattcataag aagcaacaaa ccgtagaaga
     4261 atggaagata tcgtggttgt gtagcaatcc agtgcttagt aagagagcaa cgattgaatt
     4321 tgcggacaat cgaatttcaa aattcatcgc tcaaaaggga agatgtgcag taacaggtga
     4381 ggagcttatc ttatctgaaa tgcattgtca tcatattatc ccttatcatg aaagcaaaag
     4441 tgatagctat gaaaacctag tgattgttac tgaggaggtt catcgggtaa tacacgctac
     4501 acaaagtgaa acgattgagg aacttctaaa atatctaaaa ttaaatccaa aacaaaaaga
     4561 aaaactcaat gagttgagat taaaagttgg taacgaagaa atatcttaat aaatatttgt
     4621 aggacgggaa aacagaatgt cttttataga cgagggaacg ccgtatgcgg tgaaagtcgc
     4681 acgtacggtg tggactgggg gaaaagacgg agataatatc aaactcttac ctatcagtat
     4741 cgattaatcc agattttatt gaaacggtaa attatgtcac aaactttcaa cgagaaatgc
     4801 gtaaagtaga agcaatgact gttttagcta cacaatcccc acaagaaatg gtgccagaaa
     4861 atatgtcttc tgataacttc tcgaaactga agaaagtctt tgaattttct tctacgaaga
     4921 tctttatgcg tatggatgaa tctattttac gtcatataga aggattagtg ggtaaatcaa
     4981 tgacgaactc agaattagaa tccattcctc aacaaaatca aggagacgct gtgatcaatt
     5041 ttggctcaaa agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa ttaaaattgt
     5101 ttgatggagg aaaataatgg aaaaccaaaa tgaaagttta atcaagcagt atgttaagcg
     5161 tcgtgcaaag cggcgcttat ttttgtggtt gtttggtact tctgcaggtt tgattactat
     5221 tttgatcact gtgttcgtga ccctatttct aatacttgcg gcgggctcga ttgataattc
     5281 ggattcagat tcaagcagtg gtggcgaagc ttttacagga gaatattcgg aaggcttacc
     5341 gatttataaa gaaatcaaag gaagaggccc tttttctgat gagattgcgc aatatgccgt
     5401 aggtgcagca gtgaaatata aattattacc aagtgtgatc ctttcgcaat acggttacga
     5461 atcagcgttt ggtacatcgg cgtccgcaag aaatgatctc aactattttg ggattacttg
     5521 gtttgatggt tgtctgtttc ctaaaggaac tgcacgagga attggtggca ttgaaggtgg
     5581 ctggtacatg aagtttccca actcaaaagc tgcattttcc tattacggct ttatggtagc
     5641 cacacaatcc aattttaatg cttgtgtagg aaataaaagc cctggcgcaa gcttattgat
     5701 cttaggacga ggcggttatg ccgctgcagg aattacggaa gatagtccgt attatacagg
     5761 ttgtatgtca attatcactt ctaataaatt aaccgaatat gatgaattcg ccatcaaaca
     5821 ttggggtgaa ggtggcaata acaacggcac gattactggt gaatggacga atccattccc
     5881 tggcagcagt ttagataaaa gttccttcag cggtggtcaa ttatttggaa cgaatccagg
     5941 cggtgaattc cgtccgaacg gcttccatga tggattagat tttggttcag ttgatcatcc
     6001 aggaagtgag attcatgcgg ttcatggcgg aaaagtcgtg tacgttggca atcctggtat
     6061 ttcaggtttg ggtgcgtgtg tgatcgtgat taattacgat ggcttgaata tggtgtatca
     6121 agaatttgca aatagtacag gaaattctcg agtgaaagta ggcgatcaag tgaaagttgg
     6181 tcaagtaatt gggatacgag atacagcaca cttgcattta ggatttactc gaatggattg
     6241 gcgtcaagca caaggacatg cattcattga tgatggtaca tggatcgatc cgttaccatt
     6301 tttgaatagc agtaaaaaat agatcaggag aaaaaatatg gaaaataaaa cgttaaaaat
     6361 cgtcttaaca gctgcaatta gcgttagttt aatcggcaat attgcgttgt tttggcagct
     6421 gaaagaggaa caagcagcaa atacctataa aattgaagaa gtggctaaaa acgcgtctga
     6481 gaagctcaaa aaaaattcct ctgaggcaca ggcgaagatc gacaagttaa atcaaaccat
     6541 caatgagaaa aaccaagaaa tcgatcagct gaagcaacaa gatggcacaa aagcaaaaga
     6601 ggaagtaact gaagcacaaa gaaagacggc agaagagttc ggtcaattgg ccattgataa
     6661 atcattaagc agaaatgaat taacggaaaa actaaaagat gtcgcaaccc aagaagtgat
     6721 cgacaaatta agtcctacag acgatgatca ccaacatgat gattacggaa gttactcctt
     6781 tacattaggt gacgtgcaaa cttacgctca aacaagcagt gtaaaagagg aacaaaattt
     6841 tgtcgtattt gttgattata caatcggcaa tccgcaattc aaagatgtca aaccacaaaa
     6901 aataaaaggt ggattaactg tcactgaaaa acaagtagat ggtcaatgga aagtcataga
     6961 ctttagttat ttcactcgtt aggaggaaga aaatgagtaa gaaaaaagga gaaagacaac
     7021 gtttcaaaaa acgaaaaagt tatcttagat ggctgggaat ctttatgatt ctcggctttt
     7081 tgattcttgc tagtccgatt tatacgaatc agaaggtaga tataccagat gaggtaagca
     7141 cgaacgcctt tttaaacacg aaagagctga caatggtttc aaaagaaaaa ggagcgaacc
     7201 agcaattagt ttttgaattt gcgatcgatg atgaagatca aagcttgtta aaagaactgg
     7261 ctaatcttaa ttatcgcgta gaagtaaaga cagctaaagg ggattatcaa gcaatacgag
     7321 ccaaagtgat taaggtgtct gatgattatt ttgcagtgaa gctaacgcat gtaccagaag
     7381 aatatatcgc catgcgttta accattattc ctgaaaaaat tgatccaaaa gtggatatgc
     7441 aagaaccaca agatctgatt ttttatattc acgaagataa ggtcaaagat cacgtgaaag
     7501 acgaagatta tgaacaacat gcgatcaatt acaaagtgaa aggatacaag aaagaacaaa
     7561 aagcagctca aaaacaaatg gaatctttcc aagcaaccat tgatttgaat gaagaattag
     7621 tgaagaagct aaaagatcaa ttgccttatc aagtcgcaga tgatcaggaa aatactgaaa
     7681 acaagattaa tagctaccag caagaaattg aaacatcgaa aacgcaactg aaagatcaag
     7741 aagagatcat tcaaaaactt caagaaaaaa ttgatcgtat gacaaaagaa aagatttaga
     7801 aggacaggtg gagtgaaaaa atgaagcgaa caaacttggc tgacaaagat actttcgctg
     7861 agcagaaagt attgattaaa gcctatctca aagttgtaaa agctaaagaa atatgtcgag
     7921 aagtgaattt atctaacaca acaatatctg agttgaagaa tggtcgaaag aatttagata
     7981 ccttaaaatt cagatcattt gaagctctat atgtagctgc taaaaaatat tttacagctg
     8041 ataacaaata aaaaacagga gtattttatg tgaaaaaagt atataaaaaa tgcttatgaa
     8101 tcagctatat caccttaacc atttgttaat gagatgggag aaaacattaa ccacgattct
     8161 aacgcaaaca tcaagcacat aaaacattta acactcctcc tttctggctg aagatttcaa
     8221 ccaaacagga agacagcgta tcaattatag catacacttt ttcttaaatt catttttcat
     8281 atgacaaaat atttccttct aatcgacaaa gttagaagga ttttttattg tctaaataca
     8341 caaataacct taatttttaa agagcataag gaaacgtgat atcactttct cacgaaaggt
     8401 gttaggtagg ctgtgcctac cacgtttcta agaaagataa atgatatcaa aattttggat
     8461 ttgtgtgtag atttgtgggt aggatgaaag cgaatgtggg cagtaaaata agcgtttgtg
     8521 tgtagcaaaa tcaaaaaatg tgtagggata tgtgtagcta cacatatccc tacacataat
     8581 aaatttggag gaaaatatgg acttattaat tagaaatatt gaagaaaagt atattaataa
     8641 aattgacaaa cgttgtgaag aacttacggt caagactggc aaaaaatgga gtcgaaatca
     8701 atacctaaaa gttttgatcg aaaatgattt tgatcacgct cttctgaatt ataaaaaaga
     8761 ccaatttgat cggttgttag aaaaatttgt ggatattcaa acttataata cgaaaacttt
     8821 agaagaatat attgcaacaa ataatcaatt aattggtttg ttaattgaat aataaaaata
     8881 gtattgtgaa tttataaaag gaggaaatca attttggcaa aagcaacaag aaaacatgta
     8941 tatctttacg atatgcatat cgaagtactt gatgaaataa tttctactca tgaagatata
     9001 tctaattata gtgaagcagt cagacatttg tgtatgatgt atgatccaaa aatcgaagaa
     9061 aaaataaatc caattcaaat taaattgaat gcaatgggaa aagaaatttc tattttaacc
     9121 gaaatggttt cagagtttgc ggatcttcat ttgaaagata caggaatact aattggtaca
     9181 aattcaactg tttatcaaga agcagaagaa cgtgtggaag gaaaaattaa atccaatcaa
     9241 accaaaaaat attcttctaa gttaaagaaa ccagtgaatg aatctgttca taccagagta
     9301 gatccatttg atccaacgaa gattctaaaa agataaaagt agaaggagaa cgtgatgaat
     9361 aaatcgcctg cagttattct tacttgtcaa tttaccttgc ccaataaaaa atctttttca
     9421 acatacattg attatatgac aagagaaaaa gcactggaag aaatggaacg acgtacgccc
     9481 gaagaagaac aggaactgca acgaatcaag aatgctttag aggattttta tattcctgaa
     9541 ggagaaactt tttcggaaag taagacggat aaagaattca gtgaaacgtc gaatgaagct
     9601 caatcgttga tggaacatgg aatcaatttt gacaagttag aggaacaaga ttttaccaaa
     9661 tatctttctt atatgacccg tcactatgcg ttagaacaaa agaaagaact gacgcaaagg
     9721 gaacaaaaag aataccaacg gttgaaccaa gcaatgaaaa aatatcaaac agagccacat
     9781 aaaaaagctt caggaaatct tcctggagtt ttttctatgg cttcagacga agtgaaagga
     9841 agtgatctga aggagatcaa acaaatcttc caaagaggac aacaaaaagg ctcaatcatt
     9901 tatcaagatg ttatctctca cgacaattcg tatcttgaaa aaatgggctt gtacgatccc
     9961 aaaacagata ccttagatga agaaggtttg aaagcagctg gaagaaaaat gatggagaca
    10021 ttattcgaga aagaacaatt gaatgaaaca ggttactgga tggcaacgat tcaccgtaac
    10081 actaagcata tccatattca ttttgcagtt gttgaaaaag aaaatacgcg taaaccgtat
    10141 actgagattg aaaatggtgt gagttatatc gtaccgaagg ggaagagaag acaagcaaca
    10201 ttagacgcaa tgaaaaccaa ttatgtacat gagttagaac gtttctctta tgaaaaggat
    10261 cgaatgatcg gtatggagaa acgaaattta ttgacacgaa aaagtgattt gcgtaacacc
    10321 ttaacaaaag tagtaaaaga accagcacgt tacgatcgac acgccatgaa cttattaaat
    10381 gaagtatata aaaccctacc tgaaaaacgt tcggaatgga attatggaga tgaaaatcga
    10441 accaagcttt caccatttac aaggggaaaa cttgatgagt taaccaaata tgttttaaca
    10501 aaagaacctg gatatcaaga atatatacag atttcaaaag cattgaaaga agaagccaaa
    10561 agcctttatg gggaaagcaa aagagaaaca aagaatgcag aaaaaaacgc cctctttgat
    10621 ttaaaaaagc gcacaggaaa tgcgatttta acagaactga agagacagga tagtcaaata
    10681 aaaccattaa tagaaagtgt caatcagatg caatctattt acaatgaacg atcctttcga
    10741 acactcaaac agttgccaac aaatgaatac aagtatagtt atcaagaatt cgtaaagcgt
    10801 aggaaacaat tccaacaaag agttgtcacg cagcgaagct tgcgtcaatt aaatcgaaca
    10861 attaatgcac atgaagaaaa ataccacgca agtaaagcat atgaagagca acagcgaaga
    10921 atccaaatgg aacaagaaag acaataggag gaagagaaat gaaacaatat ttagatcaat
    10981 ggaaagtcat agaaggatca ctaaaagaag aacgaatcga acaacttcct gattgtttag
    11041 aaaaagagca tttgtttcag ataagcgaaa tgttgagaaa cgaacagttt gatccaaatc
    11101 aatttttagt tgttgagtac ccagctactg gtgtgtattg ttgtaatcat gtgaagggtg
    11161 aaaaatattt tattattcaa gaatacgaag gtaagttagc gccttattat actacttggg
    11221 aaataaatga agagggaatc aataatttcc catgtgaaag cattgaagag tctatttctc
    11281 tcacggaatg ttaaataaat agagacgtaa aagagtcctg aacgtgattg tccaggactc
    11341 tttttcaatg aattgttttc cgccaattca ctgagtggtg cgattcaatt ttattatatc
    11401 aaaagaaagt gagattccaa agtgaaggga ggaagaagaa atgactcaga caacgattgg
    11461 tgttgtggtt gaaaaaagca gggataatct cgtttttgtg acagaaattc aaacaggtag
    11521 agcttttgta gtcacagaca aagctgcaaa agcgtatcaa aatggcaata ttttaacgtt
    11581 aaatctgact acgaagatgt tcgttgatgc agtagaaaat tatccatttg tttagacaaa
    11641 cgatatttta caaggaggaa acggacatgg cagaagaaaa agaaccacta gataatagtc
    11701 ggttgggaaa gtccaaaaga aaattagtac ggttgcaaaa cgaattaaat aatcaatttc
    11761 aaaagctatt tgatcaccaa aaattgacaa atgggcaacc aatgaatgac aaacgaaatg
    11821 gtcgttcatg gtttaaccag caagaaaaaa tagaaaataa aatacaaagt ttgagagaag
    11881 agatcaagca acaagaaaaa caggtagaaa aacttgaaag acaagaagag ctaaaggaaa
    11941 tgggctataa caagtacggc ggactggata tgacgatcga taatattcca agaatcaaag
    12001 aagaaatcga acgctttgaa aaaggtgaat cgactttttc agccgccacg atccgaaaat
    12061 atcaacggaa gttggaaaca ttagaacagc tgaaagaaaa atcagaaaaa ggaaaagaaa
    12121 atatcctccc tgaagtacaa gcgatcattg acagcggacg agtgacgcaa tggaaaaaaa
    12181 atccaaccat ttattttttg aaaggctatc gaaaagttgc tttagaactg aatgaaaacg
    12241 ggggatttga ggaatcaaaa aaataccaag ccacgtcgga agaggaaaaa gcagctgttc
    12301 gtgaattact tgaagaggct gcaccaactg attctctgaa ggagaaagaa aaagatcatt
    12361 tctatcgagt agaattcaac gaaaatgatc cagaaaaagg attgaaaagt tatggtgggg
    12421 agatcgtaac attagaattg atcgatcaac ttaaaacgtt ggataaccaa tacgctgcag
    12481 aagcaggtta ttataaattc tattttgaag aagtgaagaa cggagaagta gtagatcaga
    12541 tccgaatgga tctaggggat ggcgtcaaca gtaatcaccc catctattta gagttagcaa
    12601 aattgtgcga acaagtacct gagaaggaac aaaaacaatc ggtcaaaaat gaacctgttt
    12661 cgaaagaaca aatgcagtcg aaagaaacaa aaacaaaaca aaagggctat caaaaacgaa
    12721 cggcagaaga gatcaaacaa gaagttaaat ccctttcgca acaggcactg gatgcggtga
    12781 aaaagcatac ccattcttct gaagacgtca aagaactttt gaactttatg tctcgttttc
    12841 cagaacgatc ctttagaaat caattattga ttgaggaaca atttccaggt gcaacggctt
    12901 gtttaggtcg tgccgcgttg aaaaaagaag gaatctatat caaaaaaggg gaacaaggga
    12961 acaagatctt tgtcagaaaa acggtcaaag gattttatga gaaaggacgt ggctttgttc
    13021 gagaaacaga agctacacca gaagataaaa aacgaatcga aagtggtcaa atcgaagtga
    13081 tcaaaaaacc ttactacacg attgaaaaag tgtttgatat cacacaaaca cagttaaaac
    13141 ctgaagacta tccgaaaatc tttccgaacc gtgtctttga ttttcaattg gataaaaaag
    13201 gacagtcaga acttcaagct gggattcaag cggtagccaa caatattgga atacaaattc
    13261 gagatatgac agaaagtgaa gtctatcaaa gagaattagg acaagcacgt ggggcgtatg
    13321 ttcgtaacga atttactggc gaagaagaaa tcgtcatgaa tactcgaaat actcctacgc
    13381 agcatttagc tacaagtatt catgaattag cccatgcaag aatgcacaaa ttttcagaat
    13441 tagataccgc aactaaagaa ttgcaggcag aaatgacttc ttatattgtg tgcaaacatt
    13501 ttggaatgga cacttcggag aaagctattc cttatattgc agcatggacg aagaatggtc
    13561 agacgttgga tgataaggaa gcggcggaac gtgggaaaat catgaatgat gtcagccgtg
    13621 tcgcaaatga attcattcaa accatttcta atgaaatcaa tcaatacaga gaaagagaac
    13681 cagagatcca accagcagaa gtgaagcagg agcataaaaa tactgtagaa acggcagaga
    13741 ataagaatat tgtgcccgtt ggttcacttt ggatcgacaa gaaagacggg aaagtaatgg
    13801 aagaagatac gggtcgaacg ttacatttga aggatgcggc gtttattgaa gatccgcagc
    13861 ataatcaagt gattttaaaa ggaaaaagtt atggaaaaga gattcaaacg gcgttacctt
    13921 cagaagaatt tgcgctagga aatccattcc gattttcact tgaaacagtg tcagaagtgg
    13981 ccaaacgaaa aaatgaaatc tattatgaac aacaattgtc acaaaaagaa cgcgtataaa
    14041 acaagtaaaa aagactatct gaaaaacgga tagtcttttt tcttttggga ggtttgaaaa
    14101 tgaccgaaaa aaaaatgagt cttattgatc gatgtaaaca gatcgatata gtggattttg
    14161 ctcgaaacaa tggaatggct gtagtcaata aaggacgaga ctatcgctta gaagaccatg
    14221 attcatttgt ttttgatcga agaaaacaac gtttttattg gaacagtcaa aatattagtg
    14281 gcgacattat tgaattagct aaactttttt tcatagacaa agagatccaa gactctaagc
    14341 aacaatttaa agcagccact gattttattc taaagaatga agacaaaaca gaacgagtgg
    14401 aaaatcttca ttttgaaaca gaaaagtaca aagatcaccc cgttgattat caaccactca
    14461 cagaaaaagg aaggaattat ctgaaggaag aacgaaaact accagagtgg ctgattgatt
    14521 atgcagaaaa agaagggttg attgccgaac taaagccaaa atatgaacgg caaaatttct
    14581 tagttcgaga cgatcgttta gatcatgcgg tcgccttttt atggaaagat ccacagacaa
    14641 aagagacagt aggggcgagt tatcaaggaa cgttcattga ttatgaacgt tttggtgaga
    14701 gaggcacata taagcatata gataaaaatt cgacagcgaa tcatggcttt aacctaaaga
    14761 tcggtgaccc taagcagtta aaattttttg agagtagtat tgatttactt agctatgctg
    14821 cactgaatcg tgatcagtta aacgatacgt ggttagtctc tatggaaggg ttaaaacacc
    14881 atgtgatcag tcattatttt ggtgaggcag tatctgaatt gagaaagaaa caagcctttc
    14941 ctcaatccat cgagatttgt gtggataatg accgtgcagg acatattttc tacgaaaaag
    15001 aacaattaat gggcgcagtc gatccattta cgaatcagaa agtacgttgt gaacgaggaa
    15061 tcgctaatga ttggcaagtg ccaaaagaat acaaaatcat ttatgaagaa gtcgcaaaag
    15121 aagaaaaggt cacaccagaa gctattatgg ccatacacaa gacagaaaac aatctgcaat
    15181 taacgaacca actggtttcc gctcacaaag taaatgcgtt ttttggccag cagctttctg
    15241 ttaacgattc aatagaagcc attaatttga aagatatttg tagagaagtc gcaaaagaat
    15301 taaaagtttg tgaacgcgtg gatggtacgt acgattttga tcgtttttat caagaaaaag
    15361 gggatattaa tgcgcagatt cttttctctt ataaagcaga acaatactac aaaggctata
    15421 aaaatcatga acatgaattt atacctgaag taaaaaagga ctggaacgat caattgaagc
    15481 atgagatcca gcaacaagaa atcagaaagc aaaaacgtgc catgttgttt caacaaggca
    15541 gacaacaaga aagggagtga aatcgtcatg atgaaaaccg ttattttagc agaaaagccg
    15601 tcacaagcga aagcttatgc cgatagcttt tctaaagcta cccgaaaaga tgggtatttt
    15661 gagatccaag atcgattgtt tacaggagaa actgtgatca cgtatggttt tggtcattta
    15721 gtcgagttag attcgcccga tatgtacgat gaaaaatgga aacaatgggc attagaacat
    15781 ttgcctattt tccccaatca ataccactat catgttccta aagataagaa aaagcagttt
    15841 agtgtcgtca aacgacagct gcagtcagct gatacgattg tgatcgcgac tgatagcgat
    15901 cgtgaagggg agctgattgc ctggtcaatc attcaacaag caggcgccaa tcaaggaaaa
    15961 acctttaaaa gactttggat caactcctta gaaaaagaag ccatttatca gggattccaa
    16021 cagttacgag acgcagaaga gacctatcca aaatttgaag aagtacaagc acgtcagatc
    16081 gccgattggt taattgggat gaacggtagc ccactgtata gtttactctt gcagcaaaaa
    16141 ggcataccag gaagtttttc tttaggtaga gtccaaaccc caacactcta tatgatttat
    16201 caattgcagg aaaagattag aaacttccaa aaagaaccat attttgaagg aaaagcccaa
    16261 gtaatcgctc aaaatggcgc atttgatgcg aagcttgatc caaatgaaac ccaagcgaca
    16321 caagaagcat ttgaagccta tttaaaagaa aaaggtgttc aattaggaaa acagccaggt
    16381 acgatccttc aagtcgaaac agagaaaaaa agtgcggcta gcccacgtct tttttcttta
    16441 tcgagccttc agtctaaaat gaatcaactc atgaaagcta gtgccaaaga tactttggaa
    16501 gccatgcaag ggttgtatga aggaaaatat ctaagctatc cgcgaacgga taccccttat
    16561 attacggaag gggaatatgc gtatctgttg gatcacttag acgaatacaa gcatttttta
    16621 aaagccgaag cgattccgac tcctattcat acaccgaata gtcggtatgt gaataataag
    16681 aaagtccagg aacattacgc gatcattcct acaaaaacgg ttatgacagc agcggctttt
    16741 gaacaactaa gtccactgca gcaggctatt tatgaacaag tgttaaaaac aacggtggcc
    16801 atgtttgcgg aaaagtatac ctatgaagaa acaacgatct tgacgcaagt tcagcagctt
    16861 cagttaaagg caatcgaaaa ggtgccgcta gatctaggtt ggaagaagtt atttggaaaa
    16921 gagagcgacg gaaaagagaa ggaagaagag ccgctacttc ctaaagtgac aaaaggagaa
    16981 atggtgacgg tggatctaca agttcttgaa aaagaaacca agccaccaca gccttatacc
    17041 gaaggtacgt tgatcacggc gatgaaaaca gctgggaaaa ccgttgatag tgaagaagcg
    17101 caatccatat tgaaagaagt ggaaggaatc ggtacagaag cgacacgggc gaatattatt
    17161 gaaaccttga aacaaaagga atatatcaag gtagagaaaa ataagctcgt ggtgacgaat
    17221 aaagggattt tgctttgcca agccgtggaa aaagagccgc tgcttacgag tgccgaaatg
    17281 accgccaaat gggaaagcta cttgttaaag atcggtgaac gaaaaggaac acaaaccact
    17341 tttctagcca atatccaaaa atttgtctct cacttattgg aagtcgtacc agggcaaatc
    17401 caatccactg attttggatc gacgttacaa gaagtgaaag cagccagtga aaagcaagaa
    17461 gccgcgcgtc atttaggaat ttgtccaaaa tgccaggaac aagaagtctt actctatcaa
    17521 aaggtggcag catgtacgtc tgaagcctgt gatttcaaac tatggacaac gattgccaaa
    17581 aagaaactta cagcgacaca gctgaaagag atcattcaaa atagccgaac aagccaacca
    17641 gtgaaagggt taaaaggtca gaagggttcg tttgaagcaa cgattgtttt aaaagaagac
    17701 ttcactacag gctttgaatt ttctgaaaag aaaaaaacga acttcaagaa aagaacacga
    17761 cgcactacaa aataatggag ggttaatctt atgcgtaata caacacacaa acaacaagaa
    17821 atcgaaaatc aaaaagccaa aattcgttca gaacaggaaa aaatgcgtgc cagcaaacga
    17881 agactgcagc aattgaagaa ccagcagcgg attcaaaaag aaatcgaatt agatcgataa
    17941 attcctttct tgttaatata aaactaacat gttatagttg tgttagtttt atattaactt
    18001 taggggaaga gtaatgaaag acaaaatcca atgtattttt gataaacata atggcaacct
    18061 atccttgaag gatgctagaa gtgaaggatt atcgcctatg acgttagatc gattagccga
    18121 aaaggataaa atagaaaagg tagcaccagg tttctatacc ttaaaggatg cgttcgtgga
    18181 tgaattgtat ctcgctcaga gtatctattc cagagggatt ttttctcatg agactgcgct
    18241 cgatcttttt caagtaggaa catatatccc taaaaagatc aatatgatgt ttcctaaagg
    18301 atacaatgta tcaaaggaga aattagatca gtatgccatt caaccgcatt acactaataa
    18361 agaaaatttt gacctgggag tcacagaaac agaaagcttt tacggcaata aaattttagt
    18421 ctatgattta gagcgaacgt tatgtgacat gtggaatcca agatacaaag caagcgtaga
    18481 ggtaaaacaa gaagcattaa aagaatatat gacaagtacc agtagaaata caaataagct
    18541 aagaagatac atggatattt taaaatcacc aaaagaaatg actttgtata tgttgccact
    18601 ttattgagag gagtaaagat gaatgacacc gacacaattt aaagagaaaa caagaaattt
    18661 cgcagcagag aaacatttaa acccacaaat tgtccaaaga tattatatga tggaaaaatt
    18721 attagaatgt attgttgaat cgccttatca tgatgatttt gtattaaaag ggggatttct
    18781 gattggatca aaatacgggt tagaaaaccg ttcaaccgtt gatattgata cgacttatcg
    18841 aaattcgaaa ctaacaaaag aaaagttaga aactgttttt tctgaattaa cagctaatcc
    18901 tacaaaagat ggaattcgtt ttgaattaaa gggattaaca gagacgaggg aagccgatta
    18961 ttatccaggt tttcaagcaa agtttattgc ttatcttgaa aatgcacgtg taccttttaa
    19021 attagatatt acttcaggtg attctattat tccagcagca ttgatgtatg aacacaaatt
    19081 aatgtttgaa gataaaacga ttcatattcc agcttatcct acggaacaaa ttctagctga
    19141 aaaattacat gccacgttta gttttggaaa agataattcg cgcatgaaag atttttatga
    19201 cctgtatatg attccgaaat tagaacgtat cgatgagaaa aatttgtata agagtgttca
    19261 aggaacattc aaggaaagaa ataatcaatc ggtgtttaag catttgtatg atcaggaaat
    19321 gcctcaattg gtgaaagatg agggcttgag agaaaagtgg aaaagatacc aacaagaaaa
    19381 ctattttgcc aaagacattt ctttcgaatc tacactaact tccgttgatt ccctgatgca
    19441 atcaattgta gagcaagaga aggcagaacg aaaacaaaat tttaagagat cacaacaaat
    19501 gaatcaagaa atggagagat aaagatgatc aataatgtaa ctttagtagg gcgattaact
    19561 aaagatccag atttacgtta taccgcaagt ggcacagcag tggcgacttt ttccctagcc
    19621 gttaatcgta atttcacgaa tcaaaacggc aatcgagaag ctgattttat caattgcgtg
    19681 atttggcgca aaccagcaga aacgatggcg acattagcta aaaaaggcag cttgatcggc
    19741 gttgtcggac gaattcaaac acggacttat gacaaccagc aaggacaacg tgtgtatgtg
    19801 acagaagttg tggccgataa cttccaacta ttagaaagta aagcagcgac agaaagccgt
    19861 atgcacgctg atcaatcgtc cagcagtcca agccctacca cctttgaaca acgggataca
    19921 gcgacgccta acaacaatgg attgaacgct tcacagaatc catttggtgg acaatcaatc
    19981 gatattagcg atgatgatct accgttctaa gtagcatttt ctattggttg catttgctac
    20041 ggtttatgtt ataatttaga caacaaaaag aattttcaat ctataaccaa aaaccactag
    20101 ctggcgggct agtggctttc ttttacgttt ctattataag aattaacggg taggcttact
    20161 gtctttcttg gcaaatttac aatcaagcca tttgcagata tagtgcgcgg ctacttctgc
    20221 catgattgca aaaacaaaat caaaaagaat tttcaaacct ataacctcct ccccttgcca
    20281 gattaggccg gggtagcaga cagcaagact attatagcat aattgaaata tccaaatgat
    20341 tggatttttg ctataatttt agcgaggtga ttgtgatgaa caaatatgtt ggtttgcttg
    20401 ataaaatccg agtaataaaa acgcaaccgt tattggttcg ctttacttta caaacgattc
    20461 atgaatcgat taattgtgta gttgcagata ttgaaattat cgataaattg ctaattatgg
    20521 atgatggaaa atacaatatt gccgtcacag gtcacttcaa taaacgaaat cagctagtta
    20581 ttgctagcat gtatgttaga aatcccgatc attttacgcg atcgatgggc atataaataa
    20641 aagaagagtt gtagtcctaa atgaggattg cagctctttt tgttttacat agaaacagga
    20701 aaagaccttt ttaggaagac acaagtgcct tctcaattga aaaacctttc tctcactcca
    20761 accgccaccg ccgttcgaga agaaaggaag tgaacgctgc acgttcacta agatatggtg
    20821 tcttgcaaag caagacaaaa tagacaaaac ttcctctcac caaaaggttc gttcctgttc
    20881 gttatctctt tattgatttg ccagaccggc aacaagttgc cttttatcac caaaaagcga
    20941 caaggacaag ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt cgttgattta
    21001 ttctggccaa caaatcaata aagaaagaac taacgaaagg aagtaaaacc tttcctaaga
    21061 ggaggcagaa tcaaacatgc gaaagaaatt tgattattgg ggtgttccct tcagcgaatt
    21121 attcccaaac tatcatgcac cacatactgt tgaatgcgat tgtggagaaa gagcgaaatg
    21181 tatcaaaagc tatcgtttgt atcaatgtcc aacatgcggg aaaaaatata ccttgtctta
    21241 tggcgattac attttgattg atgagaaagg aaagaaacgc tgatgaaaac acaacacaaa
    21301 ttattgactg taaaagaagt cgttcaaatt ttggtggaca aagaagtcat ttctgaaaaa
    21361 gtagcagaag ctttacttat tccgcggtac tggacgatag aaaccccttt agtagaggat
    21421 gcaccgacta tgctatttga agacctagtg aagattgcag gatatgaact gcattatcaa
    21481 gaaaacatgg aaacttatga aatcgaatat tggttaacca aattagagaa ctaaaaagga
    21541 gaaaaaatat ggctcaaaat tactatgacg aatttgtcaa attacctttg gataaaatgg
    21601 cacaaaaaat ggaggatatg acgttcctat accatgaaac acgtgtaccc aaaaaacatt
    21661 acaaggaaaa attgtcagtc gcagtggaag aaatgattga atcaggtgtc gaaatgaatc
    21721 tcattgcgac gtactatcga acactggaag aactgaaaaa acaaaatgga aaatggtttt
    21781 tccaagcctt actatgcttg gaagtaggtg tcaaaccaag cactatcaaa ccttccgaat
    21841 atcaagcctt agaactgact tatgcaaaat tcgcacagat taagaaagct aaagcaatgg
    21901 ctagcgactg gctagatact tttgaaagta tcgaaaaaaa tggtgcattt tatacgattg
    21961 aatcggagga gaaagataat gaataaggtg gtttatcagc taacaacaga agatgtttcg
    22021 caatttttaa caagcaaaga acacttgaat gaagtaatca gacggattca aaaagacttt
    22081 agttgtatgg aagaaatgaa gtactttatt caaagagtgg aagaacagtt gtatatggaa
    22141 agcgaaacaa gtcaaacgaa ggtcacagtc agccataatc atttagacgt gtataaacgt
    22201 cgtcctgaaa cggtaaaaaa aggaacagag cttctacgac tagaaacaga aaaatacatc
    22261 atgaatgtgt acctagaaag gagtgagctg gtcgaaaact ttgtcagcta cacgatttgt
    22321 aggaaacact attactcaga atcagaatat tattcaattt tcgagagcat ggaattaatg
    22381 gaagaagaaa tggaagagct aaacagcaaa aatgcgacgg tagacgatta ttaccttgta
    22441 tttactaggt ttttgacgtg tgggacggaa tctatcgtgt taatgggaat ggattatgaa
    22501 ctcgctgtac ccgaagcatt tttgagggaa tatgtagaag aagtagataa cccaggcgac
    22561 gactggtaca cgactttaac tgatttttta gacacctaca cttacgacga tacagaagaa
    22621 atccaaaatg aacttgaccg taaacgaatg ccatatcaac gatttgaaag gaaaaaaggt
    22681 aaataaccat ttataccgaa aagtgaggat gtgtgaaata agaacaaaaa accttgaaat
    22741 taagggaatg gttcttattt cgcaccaatc gctccaaccg cccccgccgc ttaggtggtg
    22801 cgaaataagc gggcaaagcc cgataaccta agagaaagaa ggagatccat atgaataaaa
    22861 tcattcctct attgttcgta ctgtttgttg gcgagatact tatgcgtcat gctgcagctg
    22921 tacatttaaa cttcgtagta ttcttaggaa tactggtatt ttgtttgatc ttttggcgat
    22981 ttgtattagc ccttgtgagt ctactattca agatgattgc agccatattt ttcatagggt
    23041 tatttcgatg aaaggagaga gtactaatgc caattgcaat aggaaataaa cggttacccg
    23101 tgacattaga tgaaaagaga caaaaagaat tgcagcaact aaagcagaag tacggaaaaa
    23161 gtgaatctaa gattatgtgt attgcgttag atctattgat cgcgcaagaa aaagcaggat
    23221 ttgatatacc agcactaaaa aagtgacgtc acttttcatt tgaagaaaca aaagtgatag
    23281 cacttaattg ttcctactat ttagaatatt ttctatgtcc tttttattcc attttgtatc
    23341 aataactatg aaatctttaa agtatccaga agagattatt tttagatcaa tttttttctt
    23401 acctgattct aaagattttg ttttcttaaa catattatga tttcctttcg actattttca
    23461 tataaatgtt aacatagttt atggattcat ggaatgttaa tctaatttaa tagaaaagga
    23521 taattaagta tatacgtttt ttcttatgta tgttatacta tgtatataca gaaagaggtg
    23581 tctactatgg aaatgttacc tataaaaaaa tggggaaata gtaatggcct tagattgcca
    23641 aagtatatta tggaatatct tggaattcat acagaagaca aagtgaagat aatacaagaa
    23701 gaagtaaacg gtcagaagcg gctaattatc gaagcggcta atatagaaaa tgaattaaca
    23761 attgaacagt tatttgaaaa ttacactgat gaaagaaatc atgtgacgat tcaaaattta
    23821 ggtgaagcag taggtaatga aaaatggtga gaaaacctag acaaggcgat attctattat
    23881 tgaatacagc acctcgatct ggacacgaac agacaggtaa aagaccttat attgttttaa
    23941 gtcatgatat tattgcggat tatagtaacg ttgttacagt tgctccaatt tctagtacaa
    24001 ctagaaacta tccgctatat gtaaatataa actcagaata tgaaatgaag acaacaggaa
    24061 aagtattatt agatcaacta acgacaattg attatgaagc cagagagtgt atttttttgg
    24121 aaaaagcgca tgatagttta gttgaggagt tattgatgaa agttagaaca gtttttcaaa
    24181 aagtaagcaa ataaaataga aacagcagca tgatcaacta ttcgtgattg accatgctgt
    24241 ctttttttat tctatatacg gtttgtctcg taacattgta gtcttttgca atcttagcga
    24301 tcgcaacacc tttttttaga tcctcaacga tgtttttata aacaagacga cgttggggat
    24361 cttttgcatt ggcactgtat agcttaggac gtcctttata aacaccgttc gcttttgcaa
    24421 tcttaatgcc ttgtgcctga cgtcgtttag attcggtacg ttcttgttca gcgatcatgg
    24481 ccaaaatttg aatgatcaaa tctttaatga atttatccag gagaggattg ccaattgctt
    24541 cggccatgat cggtagactt gtgatgatca gctgcacatt tttcttcttc aagtaattga
    24601 ctgaagcgat gatctcgtcg taatttcttc caagtcgatc aatagcttcc acaataaaaa
    24661 tatcttgatc tcggacgaaa tccagtgctt cttgaaaaac aagtcgatga gtaatcgttg
    24721 cgccagattg tttctcaatg aaaattctct ctgcaccgaa ttttttcatt tcttcaatct
    24781 gccgttgttc attttgatcg atggatgaga cacgtatgta tgcaactctc aaaattttta
    24841 cctactttct gcaactattt aaaaaacacc ctaatcatac acgagaaaac cagatttttc
    24901 aatgtcgcgg tcgtgtctag ttagggtata ccttatatag acaaaaacta atgatggagg
    24961 aaatagtatg tgtcactttt ctttttaaat ggcaatattt tttgtaaata aatgtcaatc
    25021 aagaaaattt aaacgattat gacattaaaa atatatttta tgaattttct agtatataac
    25081 ttgatttatc tgttaagatt tattaagaaa acgttttctt atttttgatt tcgaggtgat
    25141 agacaatgaa aaataagttt attttatctt taggtatttt aactattgga attagtgtaa
    25201 ctggaactac aattcctaca tttgctgata gtacaaatga accaactact gtagtagata
    25261 ataaccaaga attaactcct ttagatagga acggaatagc cattggtaac ccaactaacg
    25321 acttaactgt tactgaaaat tatttaatag tttctccatt acttgctcga gcagcttcta
    25381 aaacgatacg agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc
    25441 ctcaatcacg gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt
    25501 tacaaagtta taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta
    25561 gaaagtaggg attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg
    25621 atattctaag taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt
    25681 tacaggagac acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa
    25741 gaaaaaaaag agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg
    25801 tctagtcgac attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca
    25861 ctttcttttt ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa
    25921 caatatctat gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag
    25981 gagtgatttt tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt
    26041 tacaagaaga cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg
    26101 gggcaaaaag taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata
    26161 cacttgtcgt ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg
    26221 tcaatcgact gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg
    26281 acaaatcaag ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg
    26341 agcgtaattt gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac
    26401 ggtttggtgg tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag
    26461 tggatagcgg tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca
    26521 tttatcgtta tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt
    26581 tgtcatttaa tgctgaaaat ttttgccatt tatctagaaa agtgacaata gtatgagaat
    26641 tttttgtaag tgtctactga taacttattc tcaaatgttg taaggatttt attcatttgt
    26701 taatatta
//
LOCUS       DAZKOU010000033        26704 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000033 DAZKOU010000000
VERSION     DAZKOU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.033"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACG827_001332"
     CDS             complement(104..1336)
                     /locus_tag="ACG827_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0483480.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACG827_001333"
     CDS             1591..2160
                     /locus_tag="ACG827_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0483481.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACG827_001334"
     CDS             complement(2338..2778)
                     /locus_tag="ACG827_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0483482.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACG827_001335"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACG827_001335"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0483483.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACG827_001336"
     CDS             3732..4655
                     /locus_tag="ACG827_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0483484.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="ACG827_001337"
     CDS             4731..5870
                     /locus_tag="ACG827_001337"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0483485.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="ACG827_001338"
     CDS             5863..6663
                     /locus_tag="ACG827_001338"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0483486.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="ACG827_001339"
     CDS             6663..7490
                     /locus_tag="ACG827_001339"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0483487.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7495..8202
                     /locus_tag="ACG827_001340"
     CDS             7495..8202
                     /locus_tag="ACG827_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0483488.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG827_001341"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG827_001341"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0483489.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG827_001342"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG827_001342"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0483490.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG827_001343"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG827_001343"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0483491.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG827_001344"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG827_001344"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0483492.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="ACG827_001345"
     CDS             11787..13820
                     /locus_tag="ACG827_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483493.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="ACG827_001346"
     CDS             13864..15144
                     /locus_tag="ACG827_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0483494.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="ACG827_001347"
     CDS             15354..16160
                     /locus_tag="ACG827_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0483495.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="ACG827_001348"
     CDS             16172..17392
                     /locus_tag="ACG827_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0483496.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="ACG827_001349"
     CDS             17382..18968
                     /locus_tag="ACG827_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0483497.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="ACG827_001350"
     CDS             19007..21145
                     /locus_tag="ACG827_001350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0483498.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="ACG827_001351"
     CDS             complement(21513..22496)
                     /locus_tag="ACG827_001351"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0483499.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="ACG827_001352"
     CDS             22874..24265
                     /locus_tag="ACG827_001352"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0483500.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="ACG827_001353"
     CDS             24281..25321
                     /locus_tag="ACG827_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0483501.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..26425
                     /locus_tag="ACG827_001354"
     CDS             25340..26425
                     /locus_tag="ACG827_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0483502.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26458..>26704
                     /locus_tag="ACG827_001355"
     CDS             26458..>26704
                     /locus_tag="ACG827_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0483503.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRAL"
BASE COUNT         8556 a         4444 c         5428 g         8276 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta ttagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    25981 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    26041 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    26101 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    26161 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    26221 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    26281 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    26341 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    26401 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    26461 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    26521 cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca
    26581 tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt
    26641 aaaatttatt ggggtggttt ttccattgta caagcagaaa tcaatttagt aaagagagca
    26701 ttac
//
LOCUS       DAZKOU010000034        26527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000034 DAZKOU010000000
VERSION     DAZKOU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.034"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG827_001356"
     CDS             <1..1152
                     /locus_tag="ACG827_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFQ0483504.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG827_001357"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG827_001357"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0483505.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG827_001358"
     CDS             2728..3003
                     /locus_tag="ACG827_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0483506.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG827_001359"
     CDS             3009..3776
                     /locus_tag="ACG827_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0483507.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG827_001360"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG827_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0483508.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG827_001361"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG827_001361"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0483509.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG827_001362"
     CDS             7920..8222
                     /locus_tag="ACG827_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0483510.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG827_001363"
     CDS             8225..9388
                     /locus_tag="ACG827_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0483511.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG827_001364"
     CDS             9414..12842
                     /locus_tag="ACG827_001364"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0483512.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG827_001365"
     CDS             12863..13987
                     /locus_tag="ACG827_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0483513.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG827_001366"
     CDS             13968..14414
                     /locus_tag="ACG827_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0483514.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG827_001367"
     CDS             14398..14736
                     /locus_tag="ACG827_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0483515.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG827_001368"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG827_001368"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0483516.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG827_001369"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG827_001369"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0483517.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG827_001370"
     CDS             15893..16936
                     /locus_tag="ACG827_001370"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0483518.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACG827_001371"
     CDS             17095..17823
                     /locus_tag="ACG827_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0483519.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACG827_001372"
     CDS             17939..18184
                     /locus_tag="ACG827_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0483520.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACG827_001373"
     CDS             18181..18399
                     /locus_tag="ACG827_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0483521.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACG827_001374"
     CDS             18535..19074
                     /locus_tag="ACG827_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0483522.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACG827_001375"
     CDS             19067..21340
                     /locus_tag="ACG827_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0483523.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACG827_001376"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACG827_001376"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0483524.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG827_001377"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG827_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0483525.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACG827_001378"
     CDS             complement(23167..23436)
                     /locus_tag="ACG827_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483526.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACG827_001379"
     CDS             complement(23698..23826)
                     /locus_tag="ACG827_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483527.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACG827_001380"
     CDS             complement(23906..24361)
                     /locus_tag="ACG827_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0483528.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ACG827_001381"
     CDS             complement(24499..26427)
                     /locus_tag="ACG827_001381"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0483529.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         8826 a         4605 c         5606 g         7490 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa ataggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgat
//
LOCUS       DAZKOU010000035        26478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000035 DAZKOU010000000
VERSION     DAZKOU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.035"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..516
                     /locus_tag="ACG827_001382"
     CDS             172..516
                     /locus_tag="ACG827_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0483530.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="ACG827_001383"
                     /pseudo
     CDS             517..936
                     /locus_tag="ACG827_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="ACG827_001384"
     CDS             complement(1028..1378)
                     /locus_tag="ACG827_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483531.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="ACG827_001385"
     CDS             complement(1344..2675)
                     /locus_tag="ACG827_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0483532.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="ACG827_001386"
     CDS             2931..3497
                     /locus_tag="ACG827_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0483533.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="ACG827_001387"
     tRNA            3642..3729
                     /locus_tag="ACG827_001387"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="ACG827_001388"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="ACG827_001388"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0483534.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="ACG827_001389"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="ACG827_001389"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0483535.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="ACG827_001390"
     CDS             complement(7452..7898)
                     /locus_tag="ACG827_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0483536.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="ACG827_001391"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="ACG827_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0483537.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="ACG827_001392"
     CDS             8363..8881
                     /locus_tag="ACG827_001392"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0483538.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="ACG827_001393"
     CDS             complement(8958..9914)
                     /locus_tag="ACG827_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0483539.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="ACG827_001394"
     CDS             10080..10886
                     /locus_tag="ACG827_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0483540.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="ACG827_001395"
     CDS             10883..11872
                     /locus_tag="ACG827_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0483541.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="ACG827_001396"
     CDS             11869..12873
                     /locus_tag="ACG827_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0483542.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="ACG827_001397"
     CDS             13005..13547
                     /locus_tag="ACG827_001397"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0483543.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="ACG827_001398"
     CDS             13567..14265
                     /locus_tag="ACG827_001398"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0483544.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="ACG827_001399"
     CDS             14286..14498
                     /locus_tag="ACG827_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0483545.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="ACG827_001400"
     CDS             14498..15460
                     /locus_tag="ACG827_001400"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0483546.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="ACG827_001401"
     CDS             15478..15873
                     /locus_tag="ACG827_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0483547.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="ACG827_001402"
     CDS             16036..16401
                     /locus_tag="ACG827_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0483548.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="ACG827_001403"
     CDS             16398..18209
                     /locus_tag="ACG827_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0483549.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18438)
                     /locus_tag="ACG827_001404"
     CDS             complement(18271..18438)
                     /locus_tag="ACG827_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0483550.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18686..19675
                     /gene="galE"
                     /locus_tag="ACG827_001405"
     CDS             18686..19675
                     /gene="galE"
                     /locus_tag="ACG827_001405"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0483551.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19687..21171
                     /gene="galT"
                     /locus_tag="ACG827_001406"
     CDS             19687..21171
                     /gene="galT"
                     /locus_tag="ACG827_001406"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0483552.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21195..22175
                     /locus_tag="ACG827_001407"
     CDS             21195..22175
                     /locus_tag="ACG827_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0483553.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22366..23088
                     /gene="tsaB"
                     /locus_tag="ACG827_001408"
     CDS             22366..23088
                     /gene="tsaB"
                     /locus_tag="ACG827_001408"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0483554.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            23072..23614
                     /gene="rimI"
                     /locus_tag="ACG827_001409"
     CDS             23072..23614
                     /gene="rimI"
                     /locus_tag="ACG827_001409"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0483555.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23621..24085
                     /gene="rimI"
                     /locus_tag="ACG827_001410"
     CDS             23621..24085
                     /gene="rimI"
                     /locus_tag="ACG827_001410"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0483556.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24082..25098
                     /gene="tsaD"
                     /locus_tag="ACG827_001411"
     CDS             24082..25098
                     /gene="tsaD"
                     /locus_tag="ACG827_001411"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0483557.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(25707..26408)
                     /locus_tag="ACG827_001412"
     CDS             complement(25707..26408)
                     /locus_tag="ACG827_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0483558.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         8558 a         4535 c         5526 g         7859 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaaaga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggaggggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgtat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaatg
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttcattg aatccacctc atttattatt tgttatatct attatggaac
    18481 aaaagagaaa aaaaggctag ttttacgttt gtttgtaaac ctttgcaaaa aatccttgaa
    18541 aatgtttact aaatgtttta ttataaattt accatatgaa tttacgaaat tttactttgt
    18601 ttgttttcac cataaaagta aacttcgttt tttaaattat atagaatcaa caaacacaat
    18661 tttactaatc aaaggagtgt tttttatgac aattttagta ctaggcggag ccggttatat
    18721 cggttcgcac gctgtagacc agctagttca aaaggggtat caagtagctg tggtagataa
    18781 tttgctcaca ggtcacaaac aagcagtaca tccagatgct catttttatg aaggagacat
    18841 ccgggataaa gaatttctac gttccgtatt tgaaaaggaa ccgattgaag gagtaatcca
    18901 ttttgctgca agctctttag taggagaatc tgtcgaaaaa ccattgatgt atttcaacaa
    18961 caacgtatac ggtatgcaaa tcttacttga agtcatgcac gagttcaacg tgaataaaat
    19021 cgtattctct tctactgcag caacttatgg agagccgaaa gagtctccaa tcacagaaga
    19081 tacaccagct aatcctaaaa atccgtatgg tgaaagtaaa ttgatgatgg aaaaaatgat
    19141 gaagtggtgt gaccaagcat atggcatgcg ctatgtcgct ttgcgctact tcaatgtagc
    19201 cggagcaaaa gcagatgcat ctatcggaga agatcataca ccagaaacgc atcttgtccc
    19261 aatcatttta caagtagctc ttggtcaaag aaaagccttg gctgtttatg gagacgatta
    19321 tgatacacca gatggaacat gtatcagaga ttatgttcaa gtagaagatt tgattgctgc
    19381 acatatttta gctttggaat acttgaaaga aggaaatgaa agcaacttct tcaatttagg
    19441 cagcagcaaa ggatactcag tcaaggaaat gctagaagca gctagagaag tgacaggaaa
    19501 agaaattcca gcagaaatag caccaagaag agctggagat cctagccgat tagtcgcttc
    19561 aagtgaaaaa gcgagagaga ttctgggctg gaaacctgaa tacacagata tcaaagcgat
    19621 tatcaaaaca gcatgggatt ggcatgtcag ccatccaaat ggttatgaag aataggggaa
    19681 tgagatatgt caatcagtca aacgatttgt gattttgcca cacttgcgat cgaagctggc
    19741 ggatggatgg aaatggaccg cttatattta cagaatcgtc ttttagggat ggtaggagaa
    19801 gatgctctag aaagcattga agtaagacca gttaaggaag aatctgtaga cttattggat
    19861 cagcttgtcg ctaaggcaag agaaaatggt gttctttcag aattgccagc agaacaagag
    19921 atttttgaag cgcaattaat ggattttctc acaccgccac cttcagtcgt caatgcattc
    19981 tttgcacagc actattcgaa aagcccagag gaagcaactg agtatttctt taatttaagt
    20041 aagaaaaatg actacatcaa aacaagagcc atcgcaaaaa acatcaaatt tccggcagag
    20101 tcggagtatg gtgaactgga aatcacaatc aatttatcta agccagaaaa agatccaaaa
    20161 caaatcgcag cagaacgcga ggcggtaaaa gtagactatc ctacctgcat gctttgcatg
    20221 gaaaacgaag gatatagagg tcgcttgaac tatccagccc gcaccaatca ccggattatt
    20281 cgaatgaatc tagacggtga aagctgggga ttccaatatt ccccttatgc ttattacaat
    20341 gaacattcga tcattttatc agaacaacac cgcccgatga aaatcacaaa ggaaacattt
    20401 gctagactgc ttcggattac tgaagtcttg cctcattact ttgttggttc gaatgctgac
    20461 ttaccaattg ttggaggatc gattctttct catgatcatt atcaggcagg acgtcatgaa
    20521 tttcctatgg caaaagcacc gatagaaaaa cttatcaaac tagaagatta tccgcaagtc
    20581 atcgcaggag tcgtgaaatg gccgatgtct gtgatccgtt tgcaatcaac agagaaaaac
    20641 ttgcttattg aagcagctaa ctcagtattg gaaaaatgga aagcatattc ggatgaaaca
    20701 gtatctatca gagctttttc agcagatggt acaccacacc atacgattac gccaattgca
    20761 cgaagaaggg acaattactt tgagttggac cttgtccttc gagacaacaa tgtttcagaa
    20821 gaacatcctg atgggatttt tcatccacat aaagatgtcc aacatatcaa aaaggagaac
    20881 attgggttga tcgaagtaat ggggcttgct gttttaccac ctcgtttgaa accagaacta
    20941 gtagaagtag agcatttctt attaggagaa gaaaaccaga tggctgaata tcatcgtccg
    21001 tgggcagaac aattaaaaaa agaacatcct gatttgacga aagatacggt caccgatgtc
    21061 gtacaaaaat ctgtaggtca ggtttttgca cgtgtactgg aagacgctgg agtattcaag
    21121 cgagatgaaa aaggacaggc agcgtttgaa cgatttactg cgattttata aacgtaaaaa
    21181 aggaggagag acaaatggca acaattaaag acattgcgca gttggcagga gtttctcctg
    21241 ctactgtttc aagagttctc aactatgatc ctgatctttc cgttggctta gccacaaagc
    21301 aaaaaatttt tgaagcagca gaacgcttaa attatacgaa acatcgtaaa aatgcagcaa
    21361 atactaagca aacgcttcgc ctgatccagt ggtacgacga tacggaagag cttgaagatc
    21421 tttattattt atctattcgc ctaggaatcg aaaaaaaggc agaagaagca aacgttactt
    21481 tattgaaaga aacgtgggat tccattagtg atcttccggc agatggaacg atcgcactag
    21541 gaaaattcga cgaagctcag ttattagaaa tgaaaggatc ccaaaatgcc ttgcttttgg
    21601 tagattctga cggcacttct caaggaatcg actctctagt tgtcgacttt aagtcaagtg
    21661 ttcaaaaagt cattgaacac tttgtctccc ataaagcgca acatatcgct atgcttgctg
    21721 gaacagaata tacgaagaaa aaccatcagc ggctaaaaga tcccagaatt atggcatttc
    21781 aacaagtttt ggcagaaaaa ggctacgacg aaggtccaat tatcgaagca gatttttctg
    21841 tcgagtcagg atatcaagca gtgaagcagt atttaaaaac gaatccgcat gttcctgatg
    21901 cgctttttgc agctaatgat gcattagcga ttggcgcact gaaagcgatt caagaaaaag
    21961 gtttagtggt accagaagat atttctgtga tgggcttcaa tgatgtcagt gtagcgaaat
    22021 atgtaacgcc ctcgctttct actgtaaaag tttataccga atggatggga gagctagcag
    22081 tggagacgat tcttcaatta agtaattcca aagcaccggt ttcaagaaag attattgtgg
    22141 aaacagagtt gattattcga gaatcaacga aataaaagat ggaaaagctg tgggaaaatg
    22201 gagtgatacc gcagcttttt tgtgtttcag gaaggggcaa aatttgcccg ccagcagata
    22261 atcatgtatt attgtactag ttttgccttt ggtattttca tcctcagaat aaaaaagtat
    22321 gctacaatgg aaggcgagaa tgtcatagca gaaaggatca aatatatgat tacattagga
    22381 atagatactg caaaccaaac tttggcgatc ggtgtggtag aagacgaaca aattcttgga
    22441 caaatacaaa caaatatcaa acgcaaccac agtgtaacac tgatgcctgc aatcgaccaa
    22501 ttgtttgcag atcttcaaat aagtccaaaa gacattgacc gaatcgcggt ttctgatggt
    22561 cccggttctt atacagggct tcgtataggt gtaacgacag caaaaacgat tgcttataca
    22621 ttagataaag agctagtagg agtatctagt ctgaaaacca tcgcggctaa ttgcatcggt
    22681 gtggataata ttattgtgcc catgtttgat gctcgaagaa aaaatgtata cgctggggca
    22741 taccgctgga aagaaaatag attagaaact gtacttgaag atgtacatat ttctgcttct
    22801 gatttgttca cacaactaca aaagctgaat gaagctattt atttcgtcgg gagtgattgt
    22861 cacaaattct tctctgaaat aaaggaaata ttgcctagtg cgatgattaa tacagttcca
    22921 gcatgggata ttccaagtgg tgtaactgtg gctcaattag gtgctttagt tgaaccagtt
    22981 gcagagaaac aagcttttct tcctcgctat ttaaaacgag tagaagcaga agaaaaatgg
    23041 ttggagacgc acacacctgg agacgaaagt tatgttgaaa aaatttagag cgtatgccag
    23101 aagctttttt caagataaaa tgatttatac agaaaaaaca gtcgaacaaa tcgatcgtcg
    23161 ttacgtacta agagaattga ctacagaaga tatcaaagag ctgctggatg tcgaacgaga
    23221 agtctataat ggcgaattgc catggacaaa aagtgccttt atatcagaat tgaagtcacc
    23281 gcttcctcat ctatatctag gtatctttga taatggaaga atgattggtt ttatcggttc
    23341 aagagcgttc ggtttagatt gccatatcac gaatattgcc gttcgaccgg catatcagcg
    23401 taaacgaatc ggttcgctgc ttattgacga aatcgagaac tttgctataa tgaatcgctg
    23461 tgagacgatg tcattagaag tgcgtatgag taatcaagat gcacaaagac tttatcggaa
    23521 attggggttt caagcaacaa cgatcaaaaa aagatattac actgaaacaa aagaagatgc
    23581 gttggatatg ataaaacttt tagaaaatga gtgagattag ttgatttgca gaaaaaaaga
    23641 ttttgatgaa gcgtacttgg cagaatgcgt atggatgatt agcgaaaatg cttatccaca
    23701 tggttcacct tggacgaaaa tgcagttttt ggcagacatc caacagcctc actctgatta
    23761 tctgctttta gctgaagata accagatctg tggctttatt ggatactcga aagtaataga
    23821 tgaagtcgag atcacaaata ttgctgttgc agtttcagaa caaggaaaag ggcatgcacg
    23881 gcatttgtta caattactga ttacggaaca aataaaagat tcgctgcatg tttttttaga
    23941 agtacgtcta tctaatgaag cagcaagaaa actttatgaa tcagaaaagt ttcgtatatt
    24001 aggtaaacgg aaatcatatt atcgcaatcc gacggaagat gcgatcatta tgagcagaaa
    24061 gttaaaaacg gagacgatga aatgaatgaa gaattgattc tggcgattga atcaagttgt
    24121 gatgaaacga gtgtagcggt cgttagaaat ggaacagaga tcttatctaa catcgttgct
    24181 tctcaaataa acagtcataa acgttttggt ggcgtagtac ctgaagtagc tagccgccat
    24241 cacgtagaac aaatcacctt atgcttggaa gatgcactgg tagaagctgg tgtgtcggca
    24301 gaagatttat cggcagtcgc tgtcacttat ggaccaggac ttgtcggctc gttgctgatt
    24361 ggtatttctg cagctaaggc ttttgcttgg gcacaccaat tgccgcttat tcctgtaaac
    24421 catatggcag ggcatatcta tgcagcccgt ctggttaaac cattccagtt tccattgatg
    24481 gcacttcttg ttagcggagg acatacagaa ttagtctata tgcaagaaga tggatcatat
    24541 gaaatcattg gggagacaag agatgacgca gcaggagaag cttatgacaa agttggaaga
    24601 gtattaggtc tttcttaccc aagcggaaaa gaaatcgatc agctagctca tcagggaaaa
    24661 gataattacc attttccgcg agcgatgatc catgaagata attatgattt cagttttagc
    24721 ggattaaaaa gtgcatttat caatttggtg cataatgcac aacagcgtgg agaagatcta
    24781 gacaaaaatg atttagcagc tagctttcaa gcaagtgtga tcgatgtatt gattactaaa
    24841 acacttcgag cttgtcaaaa ttatccagtc aagcaactga ttatagctgg aggtgtagcg
    24901 gccaatcaag ggttgcgtga aggattgcag gatgctttgg caactaaact ccctgaggta
    24961 gaactagtga ttccccctct tcgactttgt ggagataatg ctgcaatgat tggagcagca
    25021 gcacatgtgg aaatgcaaaa aagaaatttt gctagctatc agttgaatgc tgatccaagc
    25081 ttggttttat ctgaataaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    25141 gcccaggaaa aacggcttct catgtttttg acctggctgt tcttattacc gcgtatgcaa
    25201 gcttttgaac gccgtttaat caagagttcg taagcctgtc gtttaactct gattcacaag
    25261 gaacaatttc aaaaaacagc tcctcatgtt tatgaacatt atgacttgtg accgaagagt
    25321 tgacaggtac acaccgttta atcgaaatag ggctgtgaca cgtttttgtc aaagccctat
    25381 tttttacata cttttaatca ttattgttat tttgctattt tttacagctt atttatcatt
    25441 atccgttcct ctgattgccc tcactgagta atcgtttatt gtcgaggaat atcgaataat
    25501 ctagatcgga tgaggacaag acgcttcatc tagttaatct aacagtattt tttgcaaaca
    25561 gtgaatatta cataactgaa acacacatag aaaatctttg agtcttgtcc agtgatctaa
    25621 aactgccgga tctttcagaa gttttaggga ggaaaataag cagtgaccct gatatttatc
    25681 agggtcactg cttatttttg ttcgcgttat tctctcatag ctggagtagt agctgtctta
    25741 cagaaagtaa aacgcttttt gtcataatct aacagcccgg aatcacgtag ttgtttgatt
    25801 acttgtccag cagtttcacg tgtcgtgccg ctattcactg ctaattcttt taaggtgatc
    25861 ggataaggaa tggtgatatg accatctttt gttggtacgc ccaattcttc tttcaaaatg
    25921 aataaatttt gttttacccg gtcaaaggcg ctagaggtca atccgatttg gatacgcaat
    25981 tcatgtattt caactacttt agaaattttt tgtatcatat agatcaactg ttcgggattt
    26041 tttcgtgcta atttttcata gtaggaaaca ggaaggtaga atgtttcaat atcagtcatc
    26101 gcttgagctg tataagtgta ggtctcttct ccaaacattc ctgacatagg aaaaaggtta
    26161 tttgtattca catagtcata gtagtaataa gttgcacttt catcataacg ttctaaacga
    26221 acgagtcctt tggtcaagat atacaaccgt tgtctgggat caccatcgtc gaaaaggatc
    26281 tgtccttttt tataagaacg aagaacagta tgttccttga tctgttggaa ctcttcatcc
    26341 gagaaatgtt caaaatcagg ttgatatctg agttttgaaa agtgaaaatc gattttatta
    26401 acacgcatta aaaagtcctc tccttattgt tcactatatt tattgtcact ttttttaaag
    26461 aatttgtaaa gttttaac
//
LOCUS       DAZKOU010000036        26233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000036 DAZKOU010000000
VERSION     DAZKOU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.036"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG827_001413"
     CDS             complement(<1..1835)
                     /locus_tag="ACG827_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0483559.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG827_001414"
     CDS             complement(1832..2536)
                     /locus_tag="ACG827_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0483560.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG827_001415"
     CDS             complement(2548..4857)
                     /locus_tag="ACG827_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0483561.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG827_001416"
     CDS             complement(5052..5516)
                     /locus_tag="ACG827_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483562.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG827_001417"
     CDS             complement(5516..6142)
                     /locus_tag="ACG827_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0483563.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG827_001418"
     CDS             complement(6149..6514)
                     /locus_tag="ACG827_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0483564.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG827_001419"
     CDS             complement(6504..6842)
                     /locus_tag="ACG827_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483565.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG827_001420"
     CDS             complement(6832..7158)
                     /locus_tag="ACG827_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0483566.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACG827_001421"
     CDS             complement(7136..7417)
                     /locus_tag="ACG827_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0483567.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACG827_001422"
     CDS             complement(7419..8783)
                     /locus_tag="ACG827_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0483568.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG827_001423"
     CDS             complement(8796..9365)
                     /locus_tag="ACG827_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0483569.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG827_001424"
     CDS             complement(9337..10524)
                     /locus_tag="ACG827_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0483570.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG827_001425"
     CDS             complement(10588..12315)
                     /locus_tag="ACG827_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0483571.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG827_001426"
     CDS             complement(12312..12764)
                     /locus_tag="ACG827_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0483572.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG827_001427"
     CDS             complement(12876..13256)
                     /locus_tag="ACG827_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0483573.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG827_001428"
     CDS             complement(13253..13639)
                     /locus_tag="ACG827_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0483574.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG827_001429"
     CDS             complement(13641..13919)
                     /locus_tag="ACG827_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483575.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG827_001430"
     CDS             13967..14695
                     /locus_tag="ACG827_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0483576.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACG827_001431"
     CDS             complement(14841..15317)
                     /locus_tag="ACG827_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0483577.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACG827_001432"
     CDS             complement(15277..15633)
                     /locus_tag="ACG827_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483578.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACG827_001433"
     CDS             complement(15611..15796)
                     /locus_tag="ACG827_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483579.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACG827_001434"
     CDS             complement(15797..16069)
                     /locus_tag="ACG827_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0483580.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACG827_001435"
     CDS             complement(16066..16266)
                     /locus_tag="ACG827_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483581.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="ACG827_001436"
     CDS             16394..16606
                     /locus_tag="ACG827_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483582.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="ACG827_001437"
     CDS             complement(16639..16833)
                     /locus_tag="ACG827_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483583.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="ACG827_001438"
     CDS             complement(16846..17028)
                     /locus_tag="ACG827_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483584.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="ACG827_001439"
     CDS             complement(17025..17165)
                     /locus_tag="ACG827_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483585.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="ACG827_001440"
     CDS             complement(17655..18020)
                     /locus_tag="ACG827_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483586.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="ACG827_001441"
     CDS             complement(18060..18362)
                     /locus_tag="ACG827_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0483587.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="ACG827_001442"
     CDS             complement(18362..18661)
                     /locus_tag="ACG827_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483588.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="ACG827_001443"
     CDS             complement(18654..18818)
                     /locus_tag="ACG827_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483589.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="ACG827_001444"
     CDS             complement(18824..19093)
                     /locus_tag="ACG827_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483590.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="ACG827_001445"
     CDS             complement(19105..19854)
                     /locus_tag="ACG827_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0483591.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..20543)
                     /locus_tag="ACG827_001446"
     CDS             complement(19851..20543)
                     /locus_tag="ACG827_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0483592.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20549..21220)
                     /locus_tag="ACG827_001447"
     CDS             complement(20549..21220)
                     /locus_tag="ACG827_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0483593.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21213..21554)
                     /locus_tag="ACG827_001448"
     CDS             complement(21213..21554)
                     /locus_tag="ACG827_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483594.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21732..22430)
                     /locus_tag="ACG827_001449"
     CDS             complement(21732..22430)
                     /locus_tag="ACG827_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0483595.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22517..22987
                     /locus_tag="ACG827_001450"
     CDS             22517..22987
                     /locus_tag="ACG827_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483596.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23030..23209)
                     /locus_tag="ACG827_001451"
     CDS             complement(23030..23209)
                     /locus_tag="ACG827_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483597.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23196..23534)
                     /locus_tag="ACG827_001452"
     CDS             complement(23196..23534)
                     /locus_tag="ACG827_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0483598.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(23550..23753)
                     /locus_tag="ACG827_001453"
     CDS             complement(23550..23753)
                     /locus_tag="ACG827_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0483599.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(23768..24544)
                     /locus_tag="ACG827_001454"
     CDS             complement(23768..24544)
                     /locus_tag="ACG827_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0483600.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            24843..25163
                     /locus_tag="ACG827_001455"
     CDS             24843..25163
                     /locus_tag="ACG827_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0483601.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            25181..25612
                     /locus_tag="ACG827_001456"
     CDS             25181..25612
                     /locus_tag="ACG827_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0483602.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            25742..26203
                     /locus_tag="ACG827_001457"
     CDS             25742..26203
                     /locus_tag="ACG827_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0483603.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT         7455 a         5371 c         4073 g         9334 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtacg gtgaaacgtc taggatcaat aaaacgaatt
    20401 tcaatgactc gatcaccatc gtagccgtca tacatcgtct ttagtcgatc aatgttcact
    20461 tcctcattga tggttgcgct tatgtctttt cctttgaact ttttcagaac cgctgaatat
    20521 gaatcgatta atggtttaaa cactcatatc acttcttatc taattctttt ctcttagctg
    20581 ctattgctcg ctccatcaag gcacattgct catagcttaa ctgttcaata gtttcaacgt
    20641 tatcagctaa gagccctaat ttatctgtct gctcattaac atattcgatt aaggttttgg
    20701 tcatatcttt acccatctgc tcattgaaag cttctagaat cgtctctagc atgtttaatt
    20761 tctttgtatc gattctaggt ggtgttggaa tatcttcccc ttgaaataca tataatccca
    20821 gtccgtgtag agccaatgct ttcacaaagc atcgcttcaa tgagttattg atttgcattg
    20881 catttggttt aacaactggt tggtttcgat aatctaaaac aggaaataat tcggtttccg
    20941 tgtgtccttt aaccgttact gagacagata cataagtccc agtttcatcc ataagaaaag
    21001 gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg tagtgtttgt
    21061 actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc cacgcgtgag
    21121 cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac ttacgactaa
    21181 aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc ctccatttca
    21241 gcaatgtatt tcttacctgg tccgtaataa gagatatcaa tcaagttatc tctgtcgtac
    21301 tcttctagcg catcaatcaa gccatcttcg atgacgtaaa tatattcagg tttattcgaa
    21361 tgcttcgata gatggataag ataaacatga tcccaaatag ttacatggtt gcctaaatca
    21421 tcttgatccc aagctagttc ttcattcgtc aaaagatttc gtctgatttt tcgaccactt
    21481 gtttcctcaa ttttcggctt gccccaatca ggatcaatca aatattgatc tagagtggaa
    21541 agttcttttt ccatatgcta aaatctcctt atgatgtgtt ttctttgtga ctctttgctt
    21601 gccggcggag tcactttttt atttgttgcc atgctttttg cttgtcaata tgttgttggc
    21661 ttaggatgat tggtttatag tatttccacc agcaattagc aattgccgtc cctattctta
    21721 gcgcttcagc tctattcatt gtcatcacca aaaagtctct gttgtctgtt cagttgatca
    21781 atttccatac ggatcgcagt ttctggcaac cacatttcaa taaatgaaac agcatcatcg
    21841 aatctcttac gaggtaactc gccatatctt gggattgaaa aggtacgttt aaattcagac
    21901 caaaattttg agaatacttt tttgctgatt tcttcataag ctcggctttc ttttccccct
    21961 agaactccca taactttcat atttcctttt tgcttaattt caaactcttg ttgtccgcta
    22021 attcgcatag tatctttaag catggaaaca tcttttttaa catctttcat ttcttctaat
    22081 tgatagatca tcatatcttc aattgtttga ggaacagtat tcttgcgaat aacatcttcc
    22141 atttcgttga atgcttcaat gtatttttgt ttgaagtaga tagctttctt tcctgtaaac
    22201 cccatagcca gcaagaaaaa tccatctcta ctaatgaaga aaactcgtcg atttctgcca
    22261 tatgaatctg gttcattacc ttctacaaac atctgtccaa aattggacac atcttttttt
    22321 agtgcatcaa tatctcttaa aacatgttga tgttttttct cgaagctttc tgccacttgt
    22381 aagctcgtag tcacagcttc tttatttttc aaaattacta attcttgcat tatttcttct
    22441 ctcctttttg gtataattta gttaaaaact ggtggtgttt attttgaatt tttttcactt
    22501 aacaattttt caatggatgt caataataag ttttatattg agtacgtttt taacagggat
    22561 aaaaataaaa ggaatgcgcc ctcaactaga agttgaactt aatgcttctt acttcgctgc
    22621 agatatgatt tatacaaaag taatcatttc taactattca acagaaccag ctatgttagt
    22681 taatttagaa ctttcatccg ctaacttaaa ccgcagatgg tcagctactc catttaagaa
    22741 attaattgct aaaggaggat cagtcgatga taatagaata tactctgaag ctgtaccgct
    22801 aaatattcct cctaaaagtg ctatatcttg ttaccttgca tttgaagtag ggaaaattaa
    22861 ttttaataaa ctccttaatc accaaacaaa aatgatattt actctaaata gaacccaaat
    22921 ccacaaaatt gttgatatca aaaatacaaa cttcccaata gagaaattag tgaaagaatt
    22981 aaactgagta atagtaatat acattctagt ttctcttttc tattcatttt tagtcagccc
    23041 cctcggttgg ctttttcgct ctgtactcag cttcagcttc atcaagcccc ataaaaatcc
    23101 aaaccatgta aacaatcgtt cctatcaacg cttgcctgct tccccaaagt cctaaagcgt
    23161 agatgattag cggtgcgctg aacactagcg ctctattaaa ttttcccatc cgcttgcctc
    23221 cttaagattt ccgaaaaatt tgtttctaaa aattcaagtg ttttacttct taaaaataga
    23281 tatgtgtctc ttccttcaac tggataatag acaaatccat ttttgttttt ctcgatatcg
    23341 ataatatttc tatatcttgg gttctttaaa actctagaag taaaccaatc atattttctg
    23401 ttaatccgtt ctagcacttc tggcaacgtc atccatctac cagtatcatc agcttttttt
    23461 aactcctcat aatccacttg ggagataatt acatagcctt ctggaattgg gatttttgct
    23521 tctagatatt gcattagctg ttcctccctt tacaattcgt acatagtgat aatcgaatct
    23581 ataattctgt ttgcttctgc agaagtcttt ttaccgttta aaattaaaga taagtagctt
    23641 ttgctaattc caaatctttc agcaagcatg gtgtaagtta agaactttga actttcgaca
    23701 tattctttga ttttttctct atctcgttga gtgatttctg caatgtcagt catactaaaa
    23761 ctcctttcta accaatttcc tctaaatcca tttgagggta atatccttct ttttttagta
    23821 attcgtagat aaatagacgc cctttctgtg tccatttggt attcattaca attttagtgc
    23881 caccatcggc tttcgggatc tcagttgtat gagattttgt gtatccttgg ttcatgtgtt
    23941 ttttgcacaa taaccattgg ttaccgactt ttttctgaat acctagttta tgaagtaatt
    24001 tattcatctg ttgtggagac atcccataat ctgctgcaat ctgactaatt gttactgaat
    24061 ctgtagaaga taatatgcta tctaaatagg agattttagg ttcgtactct gcaatctttt
    24121 gttctgcgat taatcttcca gttctttctt cttttagttg agttgctaat tgaatgattg
    24181 tatctggatt aagcaaagct tcttctactt tttctggagt tagataacct ccatgttttc
    24241 taattgctgg caacacttca cttgttaccc aacgtttaaa ttttttggca gagggaagtt
    24301 ttgattttaa gattaaactg tacaaaccag attcgttgat gattgtcatc tctcttgatt
    24361 gacctgaggt cgtgattcgc gaccccatct tatcttccaa atctacatgc cgcgacaaag
    24421 catctttagt gtttgaataa cccaaaacac tcgcaacgtc tttgcctaca aaatatggtt
    24481 catcatttac taaaattgtc cgaacttcgt tttgttcgaa attaaaaatt tgcggtgtgt
    24541 tcatatttct cattcctttc ttttatattc gtaaacaaat ttaacaactt ttttctaaat
    24601 tcaattgaca gtatctgagt aatactctat actaagagca taattaaata agacataaaa
    24661 acattgattt taaagctttc ttggcggtct gcatttacat atcaatagtg ttttttgttg
    24721 tctttttagt tgttaaatct gtttacaaga tatagtatag ctcattactc agaattagtc
    24781 aaattatttc tgcgtatttt tctaaacttt ttttgtaaac atttagaaag gttgttttat
    24841 cgatgaatac ctatgaaata ataaaagact tggcgaagag gaaaaagata tctatacggc
    24901 agttagaaat taattttgga tattctaacg gttatctagc aaagtggaaa aataatactc
    24961 ctaattcaga tgaattaagt cggattgctg actatttcga tgtctctgta gactaccttc
    25021 taggtagaga agaaagagaa acccctaaac atgtggattt atcagaagac gatactgttt
    25081 tttcttttga tggaaaagaa atatctaagg agacaatgcg taaagcgatt gcaattgcta
    25141 aagctttaga ggaaaatgaa tagttggagt gatggtttgt atgtatttga aattgaaaga
    25201 aatgttgagc gaatacaatt tgaaagtagt ttatatggag atgaaagagc ctggtttcta
    25261 ctatccaaaa cccagaatca tttttttgaa tgaaaatcta tacggtgaaa ctgcagaagc
    25321 ctttcattta tctcatgaac ttgcacattt cagcgcctct cattttgaat tttcagtttt
    25381 gtacgatact tctacaactt ttcactcaaa atttgaaaca gaagcagata aaatagctat
    25441 tctaatttta ttaaatatct atattgagaa cgaactgact gatgaatctc agtttaatct
    25501 cgaaaaattt atggagtatt attctattca gaataagctc agatacactt gttatgctgt
    25561 ctgccaatgc tattttaaga aaaaatattc ttacgcaagg caatatgtat gaatacatca
    25621 agaattatta aaaaaaccga tttcagcaaa acattaaaaa agcccgtgct gcaacacggg
    25681 ctctttcctc atatatgagc ttctacaaaa aaaatcatat cacagaaatg aggaagaaaa
    25741 aatggaaaaa gagattttaa taaaatctcc aggaaaaacc ttgattaaag taactgatga
    25801 ttctatttca attattagaa aaggatttat aaatttagtt aatcaaggta ttaaaggaga
    25861 aaaaactatc ccctttaaaa atatatcagc cgtacaatta aaaaaaccag gtatgagtaa
    25921 tggttatata caatttacct tgctaggcgg aaacgaaagt cgcggaggta tattagcggc
    25981 cacgaaagat gaaaacaccg ttatgtttac aaaaaaatat tggaatgaaa tggaaaattt
    26041 aaaaaaatat atagagaagc aacaatctat attagataat aattccaaag aaacacaaat
    26101 ttcaagtgca gatgaaatta aaaagtataa agagttgtta gatgaaggga ttattaatca
    26161 agaagaattc gattttaaga aaaaagaatt gcttggttta taatattcaa tatcaaaaga
    26221 gaaagaggat cta
//
LOCUS       DAZKOU010000037        25358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000037 DAZKOU010000000
VERSION     DAZKOU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.037"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACG827_001458"
     CDS             complement(132..509)
                     /locus_tag="ACG827_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483604.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACG827_001459"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACG827_001459"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0483605.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACG827_001460"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACG827_001460"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0483606.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACG827_001461"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACG827_001461"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0483607.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACG827_001462"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACG827_001462"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0483608.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACG827_001463"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACG827_001463"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0483609.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACG827_001464"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACG827_001464"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0483610.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACG827_001465"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACG827_001465"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0483611.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACG827_001466"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACG827_001466"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0483612.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACG827_001467"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACG827_001467"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0483613.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACG827_001468"
     CDS             10661..11575
                     /locus_tag="ACG827_001468"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0483614.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACG827_001469"
     CDS             complement(11708..13273)
                     /locus_tag="ACG827_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0483615.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACG827_001470"
     CDS             complement(13266..14717)
                     /locus_tag="ACG827_001470"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0483616.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACG827_001471"
     CDS             complement(14731..15747)
                     /locus_tag="ACG827_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0483617.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACG827_001472"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACG827_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0483618.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG827_001473"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG827_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0483619.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG827_001474"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG827_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0483620.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACG827_001475"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACG827_001475"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0483621.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACG827_001476"
     CDS             21340..22764
                     /locus_tag="ACG827_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0483622.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACG827_001477"
     CDS             complement(22806..23063)
                     /locus_tag="ACG827_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483623.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG827_001478"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG827_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0483624.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACG827_001479"
     CDS             24337..25314
                     /locus_tag="ACG827_001479"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0483625.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8201 a         4317 c         5696 g         7144 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcgccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAZKOU010000038        25316 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000038 DAZKOU010000000
VERSION     DAZKOU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.038"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1877
                     /locus_tag="ACG827_001480"
     CDS             237..1877
                     /locus_tag="ACG827_001480"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0483626.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="ACG827_001481"
     CDS             2031..3353
                     /locus_tag="ACG827_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0483627.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="ACG827_001482"
     CDS             complement(3415..3846)
                     /locus_tag="ACG827_001482"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0483628.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="ACG827_001483"
     CDS             complement(3910..4746)
                     /locus_tag="ACG827_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0483629.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="ACG827_001484"
     CDS             complement(4936..5238)
                     /locus_tag="ACG827_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0483630.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG827_001485"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG827_001485"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0483631.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="ACG827_001486"
     CDS             complement(5692..5958)
                     /locus_tag="ACG827_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0483632.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="ACG827_001487"
     CDS             complement(6212..6937)
                     /locus_tag="ACG827_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0483633.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="ACG827_001488"
     tRNA            complement(7303..7373)
                     /locus_tag="ACG827_001488"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="ACG827_001489"
     CDS             7490..8242
                     /locus_tag="ACG827_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0483634.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="ACG827_001490"
     CDS             8258..8737
                     /locus_tag="ACG827_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0483635.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="ACG827_001491"
     CDS             complement(8988..10568)
                     /locus_tag="ACG827_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0483636.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="ACG827_001492"
     CDS             complement(10561..11304)
                     /locus_tag="ACG827_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0483637.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="ACG827_001493"
     CDS             complement(11480..12256)
                     /locus_tag="ACG827_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0483638.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="ACG827_001494"
     CDS             complement(12504..13340)
                     /locus_tag="ACG827_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0483639.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="ACG827_001495"
     CDS             complement(13351..14280)
                     /locus_tag="ACG827_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0483640.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="ACG827_001496"
     CDS             14472..14825
                     /locus_tag="ACG827_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0483641.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="ACG827_001497"
     CDS             complement(14899..15555)
                     /locus_tag="ACG827_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0483642.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="ACG827_001498"
     CDS             15781..17715
                     /locus_tag="ACG827_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0483643.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="ACG827_001499"
     CDS             complement(17801..19024)
                     /locus_tag="ACG827_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0483644.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20889)
                     /locus_tag="ACG827_001500"
     CDS             complement(19018..20889)
                     /locus_tag="ACG827_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0483645.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="ACG827_001501"
     CDS             complement(20910..22028)
                     /locus_tag="ACG827_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0483646.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="ACG827_001502"
     CDS             complement(22111..22944)
                     /locus_tag="ACG827_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0483647.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="ACG827_001503"
     CDS             complement(22937..23854)
                     /locus_tag="ACG827_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0483648.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(23877..24056)
                     /locus_tag="ACG827_001504"
     CDS             complement(23877..24056)
                     /locus_tag="ACG827_001504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483649.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(24081..25205)
                     /locus_tag="ACG827_001505"
     CDS             complement(24081..25205)
                     /locus_tag="ACG827_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0483650.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7808 a         5046 c         4306 g         8156 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgtttt
//
LOCUS       DAZKOU010000039        24392 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000039 DAZKOU010000000
VERSION     DAZKOU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.039"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(187..2532)
                     /locus_tag="ACG827_001506"
     CDS             complement(187..2532)
                     /locus_tag="ACG827_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0483651.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2811..3713
                     /locus_tag="ACG827_001507"
     CDS             2811..3713
                     /locus_tag="ACG827_001507"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0483652.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3924..5699
                     /locus_tag="ACG827_001508"
     CDS             3924..5699
                     /locus_tag="ACG827_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0483653.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5765..7351)
                     /locus_tag="ACG827_001509"
     CDS             complement(5765..7351)
                     /locus_tag="ACG827_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0483654.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7602..7826
                     /locus_tag="ACG827_001510"
     CDS             7602..7826
                     /locus_tag="ACG827_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0483655.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7968..9719
                     /locus_tag="ACG827_001511"
     CDS             7968..9719
                     /locus_tag="ACG827_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0483656.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9719..11503
                     /locus_tag="ACG827_001512"
     CDS             9719..11503
                     /locus_tag="ACG827_001512"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0483657.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11838..12690
                     /gene="licT"
                     /locus_tag="ACG827_001513"
                     /pseudo
     CDS             11838..12690
                     /gene="licT"
                     /locus_tag="ACG827_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            12787..14673
                     /locus_tag="ACG827_001514"
     CDS             12787..14673
                     /locus_tag="ACG827_001514"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0483658.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14714..15616)
                     /locus_tag="ACG827_001515"
     CDS             complement(14714..15616)
                     /locus_tag="ACG827_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0483659.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15846..17249
                     /locus_tag="ACG827_001516"
     CDS             15846..17249
                     /locus_tag="ACG827_001516"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0483660.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17249..18682
                     /locus_tag="ACG827_001517"
     CDS             17249..18682
                     /locus_tag="ACG827_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0483661.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18827..19753
                     /gene="trxB"
                     /locus_tag="ACG827_001518"
     CDS             18827..19753
                     /gene="trxB"
                     /locus_tag="ACG827_001518"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0483662.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19966..21693
                     /locus_tag="ACG827_001519"
     CDS             19966..21693
                     /locus_tag="ACG827_001519"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0483663.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21709..22059
                     /locus_tag="ACG827_001520"
     CDS             21709..22059
                     /locus_tag="ACG827_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0483664.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22153..23013)
                     /locus_tag="ACG827_001521"
     CDS             complement(22153..23013)
                     /locus_tag="ACG827_001521"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0483665.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23105..23533
                     /locus_tag="ACG827_001522"
     CDS             23105..23533
                     /locus_tag="ACG827_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0483666.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23695..23871
                     /gene="rpsU"
                     /locus_tag="ACG827_001523"
     CDS             23695..23871
                     /gene="rpsU"
                     /locus_tag="ACG827_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0483667.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23898..24344
                     /locus_tag="ACG827_001524"
     CDS             23898..24344
                     /locus_tag="ACG827_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0483668.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7721 a         4362 c         5191 g         7118 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctctggtttt
       61 tcacattttt acataaattt gtcttaatat cgtagatgta agcttttgaa caccatttat
      121 tcggaaaaag ggagctgtga caagattttt gtcgcagctc cctttaattc tgattaatct
      181 attagatcaa tcatctttat cgtctgattc tttggtctct ggattcgctc tagcataagc
      241 tgaagcggtg ttccagtaat ctgtatagtt ttcatcagta ccgcctacgc cgaatttaaa
      301 ggcgcttttc ttcggaccat tttttgccca aagtgactcc actgtttttc ctggcgtttg
      361 gatggatttt ttattgacag taacttttcc agctgtttgt cctgtgaacc cagatacttt
      421 tttcttcatc acatcggatg ataagtcaaa tttctcatca gtgccgaaaa tctcagggtt
      481 tgcttggtaa gcttgattga ttagattggc aagataatta gccgaacgta ttcttgcttg
      541 gctgtccatt ccagtattgt catcgtgtcc tgcccaacta ctgatagtga tagatggagt
      601 tgagacgatc agccatgaat ctcggtattc attggtagaa ccagtttttc cgacccaatc
      661 tgcatttcct agatttccgt ttaagcttga gatgacatct ttgaatggtg tagtgatttt
      721 ttcattgatc acactacgca tcatgtcatt catgatcgat gccgttgctt cggaataaat
      781 acggacagaa ttactttcat gtttataaat cacattacct gcattatctg tgatggaatc
      841 aatgatatag ccttcttcgt agactccacc atttgctaag gcctggaatc catttgtctg
      901 ttgaagcgtc gttacatcta ctgtccctaa aggagccgat tcaacgcccc aattatcatt
      961 tgctgggtag ttcatcttcg ccaagtagtg ctcgtaagcg tattgtttcg atccaccgga
     1021 attcaacaca tcttgatata agtgataagc tgggatattg ttggaccatt ccagtgactc
     1081 acggacggtt tggaaggtgt tcgacccttc attggtcgca ttgacgattt tctcaccagc
     1141 attttttcct tcttgccaag tcgttgcata atctgataca cgggattctg atccgacaag
     1201 tcccatatcg attgcaggac cgtaaacaag aaccggtttg atggatgaac cagcttgacg
     1261 ttctgtatca aaggcgtggt tattttgatt ctggctgtaa ttccgaccac ccacgaagcc
     1321 atagatacgt ccggttctgt tatccatcaa tacatttcca gtctccactt ggctaccagc
     1381 accatcatct aacatgtagc cataattttc tacgccggtt tgtaatgcag catagatatc
     1441 tttatcgatc gtcgaatgga tcgtatatcc tttgttttgg atcgtttgct gtgctttttg
     1501 ataataagct tcactgaccg atgaatctga catatcagca ttatcttttt ctgccaattg
     1561 ttgtgcaata atccttgttg cttcgttcat cacggtataa tacaagaatt ctctgtcagt
     1621 ctgttccgcg atttgttgtg ggagaaagtc ttttgtaaga tcataggctt tcgcttcttc
     1681 gtattgttct ttcgtaattt gtccttcacg gtacatactg aataaaacaa aatcttttct
     1741 ggcaagaccg gcagaaagat cttcttttag cgcgcctgta ttcgtgtatg gactatacgt
     1801 gatagggctt tgaggcaaac cagcgatata agcagcttgt ggcagagtca catcctttgc
     1861 atctacacca aagattcctc tagcagcttc ctggactcca gcaatatttt gccccttatt
     1921 attccgacca aatggtgaga cattcaaata cgtagtaatg atctcatttt ttgaaaagtg
     1981 tttttctact tgtgcagaaa gaacgatctc gtttgcttta cgtttgaatg ttgtttcatc
     2041 ggttaggatc tgttgtttga ctaattgctg tgtcaaagtg gatcctccag acgagccgat
     2101 gccagtcgct tctgaaacta gtgctcgtaa aacagcttta gggacataac cttgatgttt
     2161 atcgaagtat tcatcttctg tactgataat cgcggttttc aataaaggcg aaatcttatc
     2221 agatgaaata gtcgtccgca tcagatctgt ctggattttt gatatctctg tattatctgc
     2281 ataaacaatc tttgacgttt ccgtaatgtt tcctagttct gtctgcagtt cttttttagt
     2341 cggcaattgt gtgtcttcga caagataggc aaagtagcca gcacctattc cgatacctag
     2401 agctgcgaac aacaaaagaa gaacagtaat aaaaacagtc aatgattgaa acactctaaa
     2461 aataatctta ggaacaaacc atttttcttt ttttcttcta ttctgttttt ttcgagtatt
     2521 tttgcgtggc aaaagggtca cctcaactaa tggttttatt tcgctaatta tagcaaataa
     2581 taggacaaaa aaacagacaa ggctgttttt gacaggaatt tctttttcga atgagataaa
     2641 acgtgaggaa atgatctttt ttatgaaaga gttcttaagc ttttctttag ggaaagtttc
     2701 agttactatg gagaacaggg caaagaaaaa caaaccattt tctttgtaca tcatcttatc
     2761 aatatacagg aattgttcga aacaattgcc tttcaaggag gaatccatcc atgttattag
     2821 gctcacatgt cagcatgagc ggaaagaaaa tgctgttagg cgcggcagaa gaagcagcca
     2881 gttacggatc ttcgaccttt atgatctata caggcgctcc tcaaaatact agaagaaaag
     2941 ctatcgaaga gatgaatatt gatgccggaa aagcatttat ggcagaaaac ggactaacaa
     3001 atatcgtcgt tcatgcgcct tatatcatta atttgggaaa tacgatcaaa ccagaaatgt
     3061 ttccatttgc tgtacaattt ctgagagaag agattgtccg tgcggaagct ttaggtgcta
     3121 ctcaaatcac ccttcaccca ggcgctcatg taggagcagg ggcagaagct gggattgcta
     3181 gaattattga ggggttgaat gaagttttga caaaagaaca gacggcacag atcgctttgg
     3241 agacgatggc agggaaaggt acggaaatcg gtcgaacatt tgaggaatta gcgcaaatca
     3301 tcgatggagt aaaattgaat gaaaaattat ccgttacttt tgatacatgt catacgaatg
     3361 atgccggata taatataaaa gaagattttt caggtgtaat ggaagagttt gataaggtca
     3421 tcggattaga tcggcttaaa gtactgcata tcaatgattc taaaaatcca caaggttctc
     3481 ataaagatcg gcatgccaat atcggttttg gaacaattgg atttgacaca ttgaacaaaa
     3541 tcgtccatga tccaatgttt gacaacattt cgaaaatatt agaaacccca tatgtcggta
     3601 aagataaaaa gaaagcttac gcaccatatg gaaaagaaat ttccatgttc aaagcgcaag
     3661 cattcgatcc cgaagtgttc cctgagttat tagcagaaga agtcacactt taagttttct
     3721 acctgacagc aacaaaaaag gattgggcag tctgccttgt ccttttttgt ttaagaaaca
     3781 aaggagtgct ttttgcattc gttttcgcca tgctcctttt gccccctctt tttaaattca
     3841 gacaaacagg gtatactgat agaaaagtaa aaaacgaaaa gtttcacaaa caagtagcaa
     3901 aacgtgattg aaaggacgaa aagatgctga acattttaaa ccccttgatc gaccaactaa
     3961 aaaagaaaca tacatacttt attcttggca tagccgtatt atttacaatt attcggttgt
     4021 atttatatag taaagcaatc tatggaacag atatgtcggc gacctatgat gatcagctgc
     4081 tgattcacta tgctcaaaat attttggatg gtaaatggct aggggagtat acgactacaa
     4141 cactttccaa aggaatctcc tacagcttat ttttagtact tgcacataaa ctgcatcttt
     4201 cttatgcagt tttgttcggc atgttcagta tttttgccag tatattggtt gcactcgcgt
     4261 ttaaaccgat tgtgaagaac aagtatcttc ttttaagtat ctatttttat tttttgtttt
     4321 ctccgattag ttttacacat gaatattcca ctcgaacata tcgtaatgcc ctcgtgattc
     4381 cggctgttga gatcattatc gcttgtttat tagctattta ttaccgcaaa tacggttcta
     4441 gcagaaaatt gctgccatgg tttttagggc tttctgtcgc ttttcctttc ttttggttca
     4501 ttcgcgaaga ttctatctgg cttcttcctt ttactgtagt agctttgggt atcagtttcg
     4561 tccagatctt gctagggcaa gacttttctt tcaaagaagt aaagaagtta aaaaaacgac
     4621 tctttcttct ctcgaaacgg gaatggctga aactttcatt gtttttactt cctatagttg
     4681 gcttttccat cgtctatcag tggatcgaat ataaaaatga gacaacatac ggtattgcaa
     4741 ccatcaatga ccgttccagt ggagaatttg gacgtttgat gaaacaactg atccgaatag
     4801 atgatgaaac gaacttgaat aaagaaaaca gtgagatatg ggtctcacat gctgcactaa
     4861 ataaagcatt agccgcatct cctacattcc atactgtttc tacccgtatt gacgaattat
     4921 atacgactca cgcgtggact caaggtggaa aagtaaaaga attacccgga gatatcatct
     4981 tttgggcatt gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat gcaaaagaaa
     5041 ccaatgattt ttggaaaaaa gtcaaccaag aattagaaga agcctatcac aagggaacca
     5101 tcaaaaagaa aaaagagttc tatctgatgg gatcaggaga tggaaagacg atgagtgatc
     5161 tgccaatcgt attcgatttc cttcaatcag gatacgctta tgcatggcga tataaagatt
     5221 tccatcaagg aggaaattat agtttcggaa cgcaagaaca agtcaaacag gctcaagaat
     5281 tattaaacct tccgctaatg gagaattgga tcgatagtaa tgaaccgaga acacctaatt
     5341 tccaattaac aaaatcagca aaagtcgcga atgtcatcat aaaaatttac caagttctgg
     5401 ctattcccgt gctgattgct tcaatagcag ggatattact tatgatacta ggaaccgtat
     5461 ttgcaaaagc tgatcgtgcc tattatagtt cctctttgat tgtggtcacc ggattaatac
     5521 tgacacaatt ggtttttctt ttcggtgtct catggttttg ttcttatgcg ccagaaaaaa
     5581 aggactattt tttaagtgtc tacactggtg caggcgtgcc attaacgcaa ttcatcgtag
     5641 taacaagcat gctagttttc agtaaattaa atctgaaaaa agctttatct tctaaataaa
     5701 aagacccttg gtagctagat ttttaaccta actatcaagg gtctttgtgt ggaaaaatca
     5761 gtttttattc gatttcttct accgtttcct gcaaaagctg ttcatatttt ttgtttcctt
     5821 tatataaatc accaatcgtc catgccaaca ggagaaggac aagaacaatg attacataag
     5881 cgatgtgatc agccaatgct agttgagcgg aagtggcttg ctcgccgaag aaactttcga
     5941 tctgtcctgg taatcctcgt aagttcaagt aagttagact aatcacagag aaccagccaa
     6001 gaagttttac gatgaatgaa tttttaaatc ggctacccat ctccgcttgg ctgttggtca
     6061 tcatcagtaa aggaatcatc gaaaatggca acgcaaaagc caagaacacc tgggattcat
     6121 tcattaagtt attcaaagcg gcatgttctt cgatcgttcc ttttgtactt gtaaacaaaa
     6181 cacaaatcaa tacaggaatc acagaaagca atcgtgtgac cagtcttctt agccaaatcg
     6241 ggatacgcat atggatgaat ccttccatga tgacttgacc ggtcaacgta ccagtaatag
     6301 tagagttctg gccagaagca agaagtgcta cagcaaataa agtggacaag ataccagatt
     6361 ttgctacagc aatcaaaata ccattgctca tagctgatgg atcagataaa gcttcataca
     6421 gaccaaaaaa ggaaggatct tggacagaac cggttttgaa gacagcgaca cccatgatca
     6481 atagcagtgc attgactaga aaagccattg acaactggat attagaatcc caagtagaaa
     6541 aacgtacagc ttgtgctaca tcttcttcgt tttggtgatc gatcttccga gtttgtgaaa
     6601 ctgctgaatg aagatataag ttatgcggca tgactgttgc accaataatc cctaaagctc
     6661 cagtcagcgg agtttcgccg ccgatgaccg gtgagtctga gaatgtatga ctgttaggga
     6721 taaagccagc aaataaactg tgccaatctg ggttagacaa gccaacttga tagacaaaaa
     6781 cgaataaaat gacgaaaatt aagcagacaa ccagtgcttc gatcttacga aacccgactt
     6841 ttgtcagtag taagagaacg aaaacatcta atacagtaat aaagacggca atcaccaatg
     6901 gaatatcaaa aagtaagtag agggcgattg ctgcaccaat gacttctgcg atgtctgttg
     6961 ccataatggc taattcagtc aagatccata aaatgacacc caatgtctta cttgtccgtg
     7021 ctcggattgc ttgggccaaa tccatttggg tcacgattcc taattttgct gccatatact
     7081 gtaaaagcat agcaattaga ctagacatca aaataactga catcaataaa tattggaaat
     7141 tttgtccacc agtgatcgaa gtggaccagt tacctggatc catataacca actgctacta
     7201 atgcacctgg tccagagtaa gcaagaagtg ttttaaaaaa actttttccc tttggtacat
     7261 ccactgttcc atttacttca gctaaagata aaccatttgt atgttcaata agtcgatgct
     7321 tatgaaattg tttgttggat tgattattca agaaaaaacc acctttccta tttatctacg
     7381 attacattct acacctcaga aagaaaaaat taaagaaaaa gattcggtat gcctaatttt
     7441 ttttcgtggt gtgtcttgct ggttaatgaa agaaattttg ttcttctcta aaaaaaccac
     7501 tattttttgc atgaaactaa agaatacgcc tttctttttt caataaactt tagtaaaata
     7561 aatcattgag agcaattcgg aaatagaaag gagagattta tatggtatca acaggtattg
     7621 tcgtacttat tgctattatt gctttgctaa tcggcgcagt aggtggcttt ttcctagcgc
     7681 gtaaatatat gaaagactat ttagaaaaaa atcctcccgt taatgaggac atgttgcgat
     7741 caatgatgat gtcaatgggg caaaaaccat ctgagaagaa aattcgccag atgatgcagc
     7801 aaatgaagaa ccaagggaaa aaataggttc tgacccaaag actttttgcg ggctgatttt
     7861 ttcagtccgt tttttatttc gtaatttgtt agactattca gactattcag caaaaaagac
     7921 aagcacagac aatcctatgc tcttgataaa gacaaaggag gagtaaaatg tcgattttta
     7981 gaaaattagg ttggtttttt cgtcaggaaa agaaacatta tttaattggg attttctccc
     8041 tattcgcagt ggccttggta cagttagttc ctccaaaagt gattgggatc atcattgatg
     8101 agatcgccga taagaattta tcaataaaga tcatttcctg gtggatctta ttattgatcc
     8161 ttgcagcttt cttacaatat ctttttcgtt atatctggcg gacgaatatt tggggaagtg
     8221 cagctcggtt ggagagagaa cttcggcagc agctattcaa acattttaca gaaatggaca
     8281 atctctttta ccaaaaatat cggactggag atttgatggc tcatgcaacg aatgatttga
     8341 atgcaatcca gaatgtagca ggagctggaa tcttgacctt tgcagattca gtgattacgg
     8401 gcggaacaac gatcattgcg atgattttgt ttattgattg gcgcttaaca ctgattgcgc
     8461 tgattcctct gcctttgcta gccgttactt cacgagtgct ggggtcaaaa ctccacgatg
     8521 cttttcgtga ttcgcaagaa gctttttcta gtatcaatga taagacacaa gaaagtatca
     8581 ctgggatcaa agtcattaaa acatttggac aagaaaaaga agatctcgcg gattttgaag
     8641 aaaagattga agatgcaatt attaaaaaca aacgagtcaa ttttttagac tccttatttg
     8701 atccattcat taccttgatt atcggtctgt cttatgttgc aacaattatt ttaggtggaa
     8761 agtatgtctt ggaaggcacg atctcaatcg ggcagatggt ctcatttatt agctatatcg
     8821 gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt caacgtattg gaaagaggaa
     8881 atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa gacgcatatc gtggaacaac
     8941 tgggtgcagt gcagaccccg gcaaaaggcg agctgaatgt agctatttcc tcttttaact
     9001 atccgaaaga agaatcacca gcattagaac aaatcaaatt tgcgatccaa gaaggagaaa
     9061 cattaggtat tgttgggaaa actggtgcgg gaaaaacgac aatcatcaaa ttactgatgc
     9121 gtgaatacga tgattatcaa gggaaaatcc aatttggcgg acgttcgatc aaagaatatt
     9181 cattgaatgc tttattagga tcaatcggtt atgtgcctca ggatcacttc ctgttttcga
     9241 tgaccattcg agataatatc cgtttttcta atccaaaact ttcagaagaa gaagtagagc
     9301 atgcggcttc tttagcattt atcaacaatg aaatcaagga attgccaaaa ggttatgata
     9361 ccttagttgg agaacgtgga gtctctttat ctggcggaca gaaacagagg atttcgattg
     9421 ctcgagcatt gatcaaaaat cctgaattgc tgatcttaga tgatgcactt tctgcagtag
     9481 atgccaaaac agaagaagct atcttgcata atttaaaaga aataagaaaa gagaaaacaa
     9541 ccatcattgc agctcaccgt ttaagcagcg tgatgcatgc aaaagaaatc attgttgtcg
     9601 atcaagggaa aatcattgaa cgaggaaccc accacgagct gcttgcatta ggtggctggt
     9661 atgaacgaat gtgggaaaaa cagcaattag aagcgaagat agaaggaggc gaggcctgat
     9721 ggatgaaaat tatcaatcgg aatggacaaa atcgattcca cttaaagaac agaagaagat
     9781 catcaaacga ctgatgaagt tcgccaaacc ttttagaaga acgttcatcg ttgctatctt
     9841 atttgcgttt gccctgtctg tgatcaatgt cctattgcca aggatcatcc aaacttttat
     9901 ggatgatcat ctggctaaac agtcagctac gacacaagtt attttgtttt ttgctggtgt
     9961 gtacttgttt ggagtcatag tcaaaagtat catttggttt ttccaatggt atttgtattc
    10021 catggcttca ttgaagacct atcagtatgt tcgggtaaga ttatttgaaa agctgcatac
    10081 actaggtatg cgttattttg accagacacc ggcaggatcg accgtttctc gagtgacgaa
    10141 tgacacagaa acgctatttg aattttggta tgtctttttg atggtattga ctgggatctt
    10201 tgcagtcgtt tcttctttcg ttgccatgtt tcaaatcaat ggagaaattg ccttgtggac
    10261 tttggtgttc ttgccgattt taggaatcgt catctggtat taccagaaat tcagttctcg
    10321 tatttaccga aatatgcggg aaaaattaag tcagctgaac acgaaattga atgaatcgat
    10381 ttccggcatg cgtattatcc agcagtttcg ccaagaagct cgtttagcta aagaatttga
    10441 acaagtgaac aacgagtatt tagacacgcg ctatgcgatg atcaaaacga attcgattct
    10501 gttaagtcca atcatcaatt ttctctatgc attggcgatt gcgttggcgt tgactttgtt
    10561 tgggatcgat gcgttgcatt ctcccgtaga agttggactg atctatgcat ttacgaccta
    10621 tgtccaggaa ttttttaatc caatgtctca aatgatggac tttttgagca cctttacaga
    10681 tgggatggtt gccggtagtc gtattttgaa aatcatggat acagaagaat atgcaccgca
    10741 acaaaatcct ggagcagatc aagcgattac ttcaggtaag atcgagtttc gcaatgtaag
    10801 cttttcttat gatggagagc acaatgtatt gaacaatatc tcttttgtcg ctaatccggg
    10861 tgagaccgtt gcattagtgg ggcacaccgg aagcggaaaa agctcgatca tcaacgtatt
    10921 gatgcggttt tacgaatttt atgaaggcca aatcttgatt gatgatcatg acattcgaga
    10981 atatccaatg gaagagttac gtaagaaaat gggattggtc ttgcaagatg ccttcttgtt
    11041 ttatggagat attgcaggaa atatccggat gatgaatcca gctatcacag acgaacagat
    11101 tcgccatgca gctgagtttg ttcaagcaaa caagttcatc gatgaattac caaaaggata
    11161 tcactcaaaa gtgatcgaac acggtgccag ctattctagt ggacaacgac agctgatcac
    11221 ttttgcgcga acaatcgtga cagatcctaa aatactagta ttggatgaag caacagcaaa
    11281 tatcgatact gaaaccgaag gattgatcca agaaggattg gcaaaaatgc gacaaggacg
    11341 gacaacgata gcgattgccc atcggctgtc cacgattcgg gatgcagaat tgattttagt
    11401 tttagacaaa ggtcatatag tagagcgtgg gaatcatgaa acacttctag cgcaaaatgg
    11461 tttgtatgca gatatgtaca aactgcaaag cagtggtgtg taaaaagagg ttgtgacagt
    11521 agcatagtag cattttgccc taagcgctag agtgaaaata aaacagagct tcgcagattc
    11581 ttcatctgtg agcgttagtg gcgtagtcac ccgatttaaa aaagacgacg acagctagtc
    11641 aaaagcggct aataaccact aagaaagcgt tgacaaaagc gtttgctttc gataatataa
    11701 agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga tgagtctccc
    11761 atggttccat ggacggcttt ttgggttttt ctttttgttt tggatacagc cctatatatc
    11821 atggaaagga gcaaaacatg ctgattgaga aagtactgaa taacaacgtc gtaataacca
    11881 gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc aaaaagaaag
    11941 tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca aatgacgggc
    12001 tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta gaaatttctt
    12061 gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat tctgtgtata
    12121 ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga atatttgtac
    12181 caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc atcgggaaaa
    12241 aaaacagtga agaaaatctc ggataaatac cacatcaagt tatcagaaga tgaagccgga
    12301 tttatcgcat tgcatttagt caatgcacaa ggcattcaaa gccaacaaga caatacctac
    12361 ctgttcacta taacaataca ggatattcta aatatcgtcc gctattacta taagattaca
    12421 tttgatgaag attcagtcca tttttttcga ttcgtcacac acttacgttt ttttctgaca
    12481 cgcgtcctag atggagaaaa atcagaagtg ggagaagttt ctgaggaatt gtttcaggtt
    12541 attcaaaaaa aatataagga tacgtttggt tgcgttgaga aaatagcaga ctttttagcc
    12601 gagacgttca actaccagat gtccacggac gaaaaaatgt atttaacgat ccatactgcg
    12661 cgtttagtgc atgagaatcg ttgtaaataa ataaaaaaag gaggataaat taacaagaac
    12721 gtatgaaata agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga
    12781 atagaaatgg gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag
    12841 gaaaatatca atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa
    12901 gaaaaagcaa atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg
    12961 ggagggcagt atcaagttgt catcgggaat catgtgccag tcgtgtttga agaggttata
    13021 aaagcaggaa accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat
    13081 atgcttattg atggaatatc gggctgtttc caaccattcc ttgctatcct agctgctgac
    13141 gggatgattc gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct
    13201 ggcacatttg ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt
    13261 atcggtttaa cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc
    13321 gcagcattaa tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca
    13381 ctaacgacca tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc
    13441 attccctgga ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg
    13501 ttagctagcc aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc
    13561 ttcgttccat tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca
    13621 gtattcacat tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg
    13681 ccagttattt atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc
    13741 cattgggcaa tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt
    13801 atgacacctg ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt
    13861 aagcttagaa atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg
    13921 gggattacag agccagctat ttatgggttt acattgccac gtaaaaaaat atttattttt
    13981 acctgtgtcg gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg
    14041 aatcaaggtg gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta
    14101 acagatgtga ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttgcgctg
    14161 acttttttct tttggaagga ggaagcagat gaaacagatg atagacagaa aagatcaggt
    14221 aaagaaattg taaaaacacc gatacaagga caaatcgctc cactgaatgc agcaaaggat
    14281 gcagcttttg cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa
    14341 gtacgtgccc cttttgacgg aacagtcatg tccctgtttc cgacgaaaca tgcaatcggg
    14401 atggtttctg aagcaggtct ggaattgctg attcatgtcg gattggatac tgttcagttg
    14461 gaagggaaat attttgaaag cttggtgcag caaggagaaa gagtgaaaca aggcgatctg
    14521 ttgcttcgct tcgacaaaaa gaaaatcgaa gaggaaggat acagtttaga aacacctgtg
    14581 attgtcatca attacatgga ttatttggat attttggaaa atcaagggga aacggtcggg
    14641 ccagacgatg cacttatcac cgcactgacc tgaacggtta ggtgaaagaa tggactaggc
    14701 ttaactattc tttttagtcc acttctttat tttcaaaatt gaaaggctct ttcatgatgg
    14761 ctgagctgat agggcgttta tcaaattttt gtcctacaga aattagatgg actaaattct
    14821 gttgataatt ttctgcaaaa acacaactat ataacacgtc tagaagataa caaatcgatg
    14881 tattgatcgt aaaattacca attttcgaat acagtcgttc gcgtgtcgta agtcgcaaag
    14941 cggctgtact cattgaagat agcgttgaat caccgatact ggtcaaagct aaaattggca
    15001 catgattttg tttcaatatt tctgcggtac gaaggatcac attgttttca cccgtataag
    15061 aaatcagaat cccacacata tcacttgtgc aattgtacgc ttcatagaac gattcaccaa
    15121 aagtggtgac aatcgtgaca tccattttga tgcgcttcat tttcagcatg aaatcttgag
    15181 aaatcaaagt attcgagtta ctggcgaaaa tcacgatttt ttttgcttga agcattagtt
    15241 gtttggcttt ttgcagttgg tcatgatgaa tgagagaaag tgtatcttga atcgtcatct
    15301 gttctaaaga agccatttta tttgcgatcg tcataatacc gtctgtggtg gaaaaaggaa
    15361 gattcgcatc tacgtcttga aaattccctt caagataggc tgtttcagct aaaaaagcat
    15421 tacgaaaact gacccagcca tcaaaaccca ttttttttgc tacacggatg agtgtggacg
    15481 ggtggacata acatcgctca gcaatctctt ggatggtcat ttcgccaagt gctggagaat
    15541 cgcttaagag aaactgaatc acttcatttt ctgaaggaga aaaggagatt tctttgattt
    15601 tttcacgtaa taacatgtag ataccttctt cttgtttttt tacgtaaatc ataacttttt
    15661 tgaaaatgat ttgtaaacgg atttatgtta tatagcttaa taaagacatt ttttgacaag
    15721 atatttacaa aaatccagaa gaaaacacta acttttgtaa atgttactgg aaagaaacga
    15781 atttctattg tttgttttgt ttacaaaaaa gataataaaa gtgtaaaaag taaaggagtg
    15841 agaacatggc aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg
    15901 agttgatgga aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg
    15961 tagatgtaga agaaggaaaa gaaaagcttg agatcgtcag tcagatgtct cagcgtatgt
    16021 gggatgcttc tccgtatgaa gtcaaaatct atgcaactct ggatcgggaa gaagcattga
    16081 aagatgcgga ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag
    16141 acgagcggat ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga
    16201 tattcaaagc ttttcgtacc gttccagtgg ttttggcaat cgttgaagac atgaagcgtt
    16261 tatgtccaaa tgcttggctg attaatttcg cgaacccaag cgggatggtg acagaagcag
    16321 ttatcagata tggcaaatgg gataaagtga tcggattatg caatgtgcca gtaatggcga
    16381 tgatgacaga accaacaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg
    16441 gtctaaatca cttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag
    16501 aattgattga tcgtttatac gatgaagaca atggattgcc gaaaaatatc tttgacatcc
    16561 cgtttttcaa agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt
    16621 actatcgtga agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa
    16681 gagcgcagca agtgaaagaa acagaagcag agctgtttga attatacaaa gatccagaat
    16741 tggatcataa acctgaacaa ttgcaaaaac gaggtggtgc ttattattca gatgctgcct
    16801 gtgaaacgat tgcttcgatc tatggaaata aagaaacaca gatcgtggta tctacaaaga
    16861 atgatggagc tgttcctgat ctgccggcag attgtgtagt agaagtcact gcctatgttg
    16921 gcgcacaagg cgcacgaaat gtagcgtttg gttcccttcc tgctgctgaa agaggctggt
    16981 tgcaagtaat gaagaatatg gaattgctga cgatcgaagc agccgtgact ggtgattatg
    17041 gaacagcttt gcaagcgttt acgatcaatc cgcttatacg atcaggaaag acagcaaaac
    17101 ggatcatgga tgagttgttt attgcacatc agcagtattt accgcaattc aaggaaacaa
    17161 tcagccggtt aaaagcagaa ggaatcgaag tccaagatga actggcaaga gaattatcaa
    17221 gcaatacaca tgtaatggag gaagtatgat gggattcaga aaagattttt tatggggcgg
    17281 agctacagca gccaatcaat gcgagggcgg ttatgatgaa ggcggacgtg ggttagcgaa
    17341 cgtagatgtt gtgccaatcg gagaagaccg ttttcctgtc attactggaa aaaagaaaat
    17401 gtttgaattt gatgaaaatc atttttatcc agcaaaaatt gcgatcgata tgtatcatca
    17461 ttataaggaa gatattgctt tatttgcaga aatggggttc aagacttatc ggatgtccat
    17521 tgcttggagt cgtatttttc caatgggcga tgaaaaagag ccaaatgaat ctgggttgaa
    17581 attttatgag gatgtcttta aagaatgtca aaagtacggg atcgagccgc ttgtgacgat
    17641 tacacatttt gattgtccga tgcatttgat caaagaatat ggtgcgtggc gttccagaga
    17701 actcgttggt ttttatgaaa atctgtgccg agtcatcttc aatcgctata aaggacttgt
    17761 caaatattgg ttgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc
    17821 agggctgtat tttgaagaag gagaaaatga agaacaaatc aaatatcaag cggcacatca
    17881 tgagttggtt gcaagtgcca tcgcaacgaa gatcgctcat gaagtagatc cggagaacaa
    17941 agtaggctgt atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt
    18001 ttgggcttct agaaagtcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg
    18061 agaatatccc gcttatgcat tgaaagaaat ggaacgaaat ggcattcaga tcaaaatgga
    18121 acctggggat gccgcattgc tgaaagaaca tactgttgat tttatctcct tttcttatta
    18181 ttcttctcga gtttcgacta ctgacaacga actcttggaa cagactgcag gaaatatctt
    18241 tgcttctgtc aaaaatccat atctggaagc aagtgaatgg ggttggcaaa ttgatccatt
    18301 aggcttgaga ataacgatga atgatattta tgatcgttat caaaaaccac tatttattgt
    18361 agaaaacggc ttaggtgcag tcgatacgcc cgatgagaat ggttatgtgg aagatgatta
    18421 tcgaatcgac tatctggcag cacatatcca ggcaatgaaa gatgcagtag aattagatgg
    18481 agtcgatcta ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtacagg
    18541 tgagatgaag aaaaggtacg gattcattta tgtcgatcgt gacaacgaag gaaatggcac
    18601 attagcccgt tcgaagaaga aatcattcaa ctggtataaa aaagtaattg attcaaatgg
    18661 agaaaatctt tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt
    18721 tttcgagtat aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat
    18781 tggctgtaaa gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat
    18841 cgttattggc gcagggcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt
    18901 atccgtattg atgatcgaac aaggcgctcc tggcggccaa atgaacaata cggcagaaat
    18961 cgaaaactat cctggtttcg attcaatcat gggtcctgat ctagcttata agatgtatga
    19021 aggtgtttca agatttggga cagaaaatgc ttatggaatc gtgcaagata tcaaggatca
    19081 cggcagccac aaagaagtta tttgtgaaga taaaacttac gaaggaaaaa cagtcattat
    19141 cgcaacaggc tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg
    19201 tggcgtttct tactgtgcag tttgtgatgg tgcatttttc cgtaacaaac gtctagtagt
    19261 gatcggagga ggagactcag cagtagaaga agccatctat ttgacacaat ttgcttctga
    19321 agtagtcatc gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc
    19381 atttgcgaat gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa
    19441 tgaaatggta gtgacaggtg tgaaagcacg taatgtcaaa actggcaatg agtttgattt
    19501 ggcagcagac ggagtattca tctatgtagg attggatcca ttaactgcac catttagaga
    19561 agctgggttg acgaacgaag ccggctggat tccaacagac aacgaaatga agacgaatat
    19621 accaggcgtt tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactactgc
    19681 tgttggtgat ggtgggattg ctggacaaca agtgtataaa tacttggaat ctttagaaga
    19741 aacaactgca taagtttaaa aagagtgaaa gaacggtttc aattgttctt ttactctttt
    19801 ttttgcgtga aatctactga atccgattta tgcgaatact tgtaaccgtg cttgataaaa
    19861 gaatcttttc ttgatttaag cgttttttgg tcctcatact ttcatatcta ataagtgaat
    19921 gctatactta ttaagaacca tatttcagaa agaggtgtta ttttcatgtc ttgggaacaa
    19981 gtgtatgaac aatgggtaca tgaagaaaat ttagaagaaa acttaaaaaa acaattaaat
    20041 gatctgggtg aagatcctga aaaacttgaa gatgcttttt atgcaccgtt ggaattcggt
    20101 actgcaggca tgcggggaat cttaggacct gggatcaatc gaatgaacat ctatacggta
    20161 agacaagcaa cagaaggact tgcacgtttc atggatacac aaggtgaaga aacaaaacgc
    20221 cgaggtgtcg ctatcgctta tgactcacgc catcaatcac cagaatttgc catggaagca
    20281 gccaaaacat tagcacatca caatatccct tcttatgtat tcgaaagttt acgtccaaca
    20341 ccagagcttt cttttgcagt ccgtcatttg aagacgtttg cgggaatcat ggtaactgca
    20401 tcccacaatc cagcagctta caacggatat aaagtctacg gagaagatgg gggacaaatg
    20461 cctcctgcag acgcagatgc attgacgaaa tatgtccgtg aagtcagcaa tccgttaaaa
    20521 gttgaagtat tatctgacga cgaagctgaa catagcggat tgatcacgat catcggagaa
    20581 gaagtggatg ccgcttatct agaagaaatc aaagcagtaa cgattgatca tgaattggtg
    20641 gaaacaatgg gtaaagattt gaagcttgtg tacacaccac ttcacggaac aggaaaaatg
    20701 ttgggcgaac gtgcgttgaa acaagctgga ttcgaacaat tcgtactagt tccagaacaa
    20761 gctgtcgcag acccggattt cactacagtc aaatcaccga atccagaaga acactctgct
    20821 ttcgagtatg cgatgcgact tggcgagaaa gaaggcgctg acttactgat tgcaacggat
    20881 cctgatgcag accgtttagg cgcagccgtt cgtttgccaa atggctccta tgaagtattg
    20941 acaggaaacc aaatcggagc cctgtttgct caatatattt tggaagcaca ccaatcgcga
    21001 ggcactttac ctgaaaatgc agcggtattg aaatcaatcg tttctagcga attgcctaca
    21061 gcaattgcgg aacactatgg ggctaccatg ttcaatgtat tgacaggatt caaattcatt
    21121 gctgaaaaaa ttcaacaatt tgaagaagat cattcccata cttttatgtt cggattcgaa
    21181 gaaagttacg gttatttagt taaacctttc gttcgcgaca aagacgcgat tcaagcattg
    21241 ctcttattag cagaagtagc agcttactat aaaaaacaag gcaaaacatt gtatgacggc
    21301 ttgcaaacga tttttgaaac atatggctac tacgcagaaa aaacgatttc tgtgacaatg
    21361 agcggacaag aaggctctgc aaaaatctct gctttgatgg aaaaattccg tgaagaagca
    21421 ccgacagaat ttgccggagc aaaagtcatc caaacagaag acttcaaaga gttgacgaga
    21481 acagaccgtg aaggaaacgt agaaaaacta acgacaccgc catctgacgt attgaagtat
    21541 acgctggaag aaaatggctg gatcgctatc cgtccttcag gaactgagcc gaaaatcaaa
    21601 ttttatatcg gtgtaaaagg aaactcttca gaagacgcag ctgaaaaagt agccgcttat
    21661 gaagcgatta tcaaaaaaat gacagaagaa taaaaaaact tgagactgat ggaaaaagtg
    21721 gtaaacttta gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa
    21781 aaaacaaaag tagagaaagt cagccaacta ttcaaaatgt tgagtgaccc cactcgtctc
    21841 aaaattctgc tgtatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagta
    21901 gaaatggaac aatctgctgt atcgcatcaa ctgcgtttat taagagaaaa ccatgtcgtc
    21961 aagtcccatc gtgagggaaa agcgattttg tatagtcttg atgattctca cgtactggat
    22021 attttgaacc agacgctcaa acatgtggaa catcaataac gataggtgca aatagagaca
    22081 aactggactg tatcaacgaa tcttcttcgc tgacacagtc cagttttttt aagaaaaaga
    22141 caagaaaaac tgttagtctg tttcttcggt ggtttcagtt ccataataac tatgatcttc
    22201 taggtctaaa gcattcaaaa taagtgaagc tgtttcttcg atagataaag aagcgacatt
    22261 gattactacg cagcccaatt tttcataaag atcattcgca aaatcgagtt cagctcgtat
    22321 tttttcgata tcggaataag cagtatcagg atttaatccg taagagcgca tccgttcttt
    22381 acggatattg tttaaaatat ctgggttatt cgtcagtccc acgatttttt taggatcgat
    22441 ctcccaaagc tgtttgggaa tatgtgcttg tgggatcaaa ggtagattgg caacctttaa
    22501 atttttgtta gctaaaaaca agctcaaagg cgttttggaa gttctggata cgcctagcaa
    22561 aacgacatct gcttctagaa acccacgagg atctttcccg tcatcgtatt ttacagcaaa
    22621 ttccattgct ttgatccgtt tgaaatagtt ctcattcaat aaatgaaggg cgccaggttg
    22681 ccgaatgggc ttgataccag ttaagcgctc gatttctgat atcggcggag tcatcacatc
    22741 aaaactaaat aattgatgtt cttcacaaaa agaacgcgtc atctcgatca attcttgatt
    22801 gatcaatgtg tgtaggacaa gggctttttc ttttagtgca tcttgcagtg cctgcatcaa
    22861 tgttttctca tctgtcacaa atgttctgcg gaataaattg aactcaacgg taggatattg
    22921 ggccatagtg gcttgcgcta attttgtcgc agtttctcca gcagagtcag agatgacaaa
    22981 aaaagtaatg atttctgtag aatcggaaga catggataaa acctcacttg catttttttt
    23041 atgtttatta taccatactc tagaatgtgt acttcgtaat cattttgatt tggaggaata
    23101 agtcatgaaa aaacagacat tgattgaaca atcactagaa acattgaaaa ataacggttt
    23161 taaatatact aaaaaaagag aagcattgtt aacttattta gtgaagcgca atcgctacgt
    23221 atccgctaaa gaagtttttg actatatgaa tcaggaattc aaaggagtaa gttacgatac
    23281 gatttatcga aatctccatg attttgcaga aatcgattta ctggaagaaa ccgatttaaa
    23341 tggagagatg aagtttcgtt ttcattgctg tcaaggggaa atcggccatc accatcactt
    23401 tatctgtacg gtgtgcggaa caacgaaaga aatccatatg tgtccaatga attatttcga
    23461 agaacagctc ggtggatgta cgatcgaagg gcatagattc gagattttcg gaagatgtga
    23521 aaattgtcaa taaggattta aataaaataa catttgataa ttttaaaaaa aagcaagatt
    23581 tccttgacgg tcaaaacagc attagatata atatttacag gacagtcttg ttttttgaga
    23641 tatatttcaa taacaattac ggttactttt taagctcgga gggagggaat taacatgtca
    23701 aaaacagtcg ttcgcaaaaa cgaatcactt gacgatgctc ttcgtcgctt caaacgttcc
    23761 gtgtcaaaag cgggtacttt acaagaatct cgtaaacgtg aattctacga aaaaccaagt
    23821 gtaagacgca agaaaaaatc tgaagcagct agaaaacgca aaaaattcta gttctgtgat
    23881 gaattggagt tggtagtgtg tcactactaa gtagattaaa cgatgacatg aaaacagcga
    23941 tgaaagcgaa agataaggaa tctttacaag tcatccgaat gatcaagtca tctatacaaa
    24001 acgagcagat taaagaaggt cacgacttga cagaagaaga agaattaacg gtgttgtctc
    24061 gcgaaatgaa acagcgacgt gattctttgc atgagtttga agaagcagga cgtgacgatt
    24121 tagctgaaaa agtaaaaagt gaaattgtga tagtcgaaaa atacatgccg gaacaacttt
    24181 ccgacgaaga aattcgacaa cttgttcaag aagcaatcac acaaactggt gcttcatcaa
    24241 tgaaagaatt tggcaaagtc atgggtgcga tcatgcctaa agtcaaagga aaagcagatg
    24301 gaaaccaagt aaatgccatt gtgaaagaac tattacaatc ttaattttat catttaaagg
    24361 ctcttcgtca atgagtgttg gtgaagaaat tc
//
LOCUS       DAZKOU010000040        24104 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000040 DAZKOU010000000
VERSION     DAZKOU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.040"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACG827_001525"
     CDS             complement(55..789)
                     /locus_tag="ACG827_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0483669.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACG827_001526"
     CDS             1116..1526
                     /locus_tag="ACG827_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0483670.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACG827_001527"
     CDS             1519..2220
                     /locus_tag="ACG827_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0483671.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACG827_001528"
     CDS             2220..3806
                     /locus_tag="ACG827_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0483672.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACG827_001529"
     CDS             3825..4046
                     /locus_tag="ACG827_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0483673.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACG827_001530"
     CDS             4043..4804
                     /locus_tag="ACG827_001530"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0483674.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACG827_001531"
     CDS             5184..5693
                     /locus_tag="ACG827_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0483675.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACG827_001532"
     CDS             complement(5768..6307)
                     /locus_tag="ACG827_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0483676.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG827_001533"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG827_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0483677.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACG827_001534"
     CDS             7320..8171
                     /locus_tag="ACG827_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0483678.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACG827_001535"
     CDS             8199..8834
                     /locus_tag="ACG827_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0483679.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACG827_001536"
     CDS             8854..9627
                     /locus_tag="ACG827_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0483680.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACG827_001537"
     CDS             10035..10832
                     /locus_tag="ACG827_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0483681.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACG827_001538"
     CDS             10810..11223
                     /locus_tag="ACG827_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0483682.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACG827_001539"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACG827_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0483683.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACG827_001540"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACG827_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0483684.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACG827_001541"
     CDS             16000..16560
                     /locus_tag="ACG827_001541"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0483685.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG827_001542"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG827_001542"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0483686.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACG827_001543"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACG827_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0483687.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACG827_001544"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACG827_001544"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0483688.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG827_001545"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG827_001545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0483689.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG827_001546"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG827_001546"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0483690.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACG827_001547"
     CDS             21907..22485
                     /locus_tag="ACG827_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0483691.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACG827_001548"
     CDS             22537..23019
                     /locus_tag="ACG827_001548"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0483692.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACG827_001549"
     CDS             23136..23510
                     /locus_tag="ACG827_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483693.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23885..>24104)
                     /locus_tag="ACG827_001550"
     CDS             complement(23885..>24104)
                     /locus_tag="ACG827_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0483694.1"
                     /translation="PSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIF
                     DTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT         7861 a         4324 c         5168 g         6751 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgggagcgt caataatttt gtgtaaataa attgtcctcc tgcaaaataa
    23881 ttagttactc agtaaacatt gaaactaatg tatcggttac ctgttgaaaa cctttatggc
    23941 ttctgtttag aaatttttga ttgtatgtat caaaaatgct gactagaaag cgttctagtg
    24001 attcttcatt ttgaaactgc tcttttctac ggctgtatct tttaatttgc ttattgaaag
    24061 actcgattag attggttgag taaatggttc tacgaatgct aggt
//
LOCUS       DAZKOU010000041        23932 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000041 DAZKOU010000000
VERSION     DAZKOU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.041"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..3331
                     /locus_tag="ACG827_001551"
     CDS             53..3331
                     /locus_tag="ACG827_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0483695.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3872..5779)
                     /locus_tag="ACG827_001552"
     CDS             complement(3872..5779)
                     /locus_tag="ACG827_001552"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0483696.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5909..6784
                     /locus_tag="ACG827_001553"
     CDS             5909..6784
                     /locus_tag="ACG827_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0483697.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6864..7838
                     /locus_tag="ACG827_001554"
     CDS             6864..7838
                     /locus_tag="ACG827_001554"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0483698.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8018..9163
                     /locus_tag="ACG827_001555"
     CDS             8018..9163
                     /locus_tag="ACG827_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0483699.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9300..9644
                     /locus_tag="ACG827_001556"
     CDS             9300..9644
                     /locus_tag="ACG827_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0483700.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9915..11201
                     /gene="efmA"
                     /locus_tag="ACG827_001557"
     CDS             9915..11201
                     /gene="efmA"
                     /locus_tag="ACG827_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0483701.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11339..11938)
                     /locus_tag="ACG827_001558"
     CDS             complement(11339..11938)
                     /locus_tag="ACG827_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0483702.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12342..13463
                     /gene="mnmA"
                     /locus_tag="ACG827_001559"
     CDS             12342..13463
                     /gene="mnmA"
                     /locus_tag="ACG827_001559"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0483703.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13709..15130
                     /gene="arcD"
                     /locus_tag="ACG827_001560"
     CDS             13709..15130
                     /gene="arcD"
                     /locus_tag="ACG827_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0483704.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15144..16262
                     /locus_tag="ACG827_001561"
     CDS             15144..16262
                     /locus_tag="ACG827_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0483705.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16322..17614)
                     /locus_tag="ACG827_001562"
     CDS             complement(16322..17614)
                     /locus_tag="ACG827_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0483706.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17764..18324
                     /locus_tag="ACG827_001563"
     CDS             17764..18324
                     /locus_tag="ACG827_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0483707.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18453..18830
                     /locus_tag="ACG827_001564"
     CDS             18453..18830
                     /locus_tag="ACG827_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0483708.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18763..20835
                     /locus_tag="ACG827_001565"
     CDS             18763..20835
                     /locus_tag="ACG827_001565"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0483709.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21046..21993)
                     /locus_tag="ACG827_001566"
     CDS             complement(21046..21993)
                     /locus_tag="ACG827_001566"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0483710.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22239..22703
                     /locus_tag="ACG827_001567"
     CDS             22239..22703
                     /locus_tag="ACG827_001567"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0483711.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22765..23808)
                     /locus_tag="ACG827_001568"
     CDS             complement(22765..23808)
                     /locus_tag="ACG827_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0483712.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         7771 a         4393 c         5050 g         6718 t
ORIGIN      
        1 ttaggtaata ctttaagtaa acgctttcta atttgttacg ggaggaatac acttgttaaa
       61 aaaacgtata aaatacagtg cgctaatgat gaatctcctt atgctcagta ccccggtttt
      121 aatttctgtc gttgaagttg ctgctaacga acaacagtct gtgaacgaag aaaatgcaca
      181 cgtgaacatt gaagggatgt caacaatgga ggaaatacca agaactagtg aagatatgga
      241 tacggaagaa ggaaatgatc ctatgatgaa agaatcagaa aatgaaaaga caaaaaagca
      301 atctgaagaa gcaaatgaag agtggaaagg ggttgttttc ggagaaagca caagtgctag
      361 taggtatgcc attgaaccat tagaagacgg cgtgcggctc tcttccacga ataacggcgg
      421 aaaattccag tcaagcggat cagatggaat gacctattat tatcaagcaa tccccaaaga
      481 catcaacttt aggatgcggg cgacgatcga aatagaatct tggacctata cgaatgccca
      541 agaaggattt gctttaatgg taagagacgc agtaccaaaa aatcatttgt ttgggcaggc
      601 attttattca aattcatttg ccatactagg cagtcggatc gaatatgtct gggactcgga
      661 tagacaagaa gtggtcaaca cgagcggcag caaatatacg atgcgacttg gattggggac
      721 acgggcaatc acaggaatat catctgaaga tccgcaagct gcgccagctc ctgggagtgt
      781 ctcagtggaa acaacacctt tagagacttc agcagggttc ttggaaaaag aaacaggttc
      841 ttataatata ttcggaaacg gtcatgggaa tctgtttccg gccacgaact cgattactaa
      901 actggacgta gagatgaaaa aaacgaatac tggtttagag gcatattatt acgatcctga
      961 aacaggagaa gaaatggctt ctgacatcat gtatgactgg gaaaaattat acgcttcaga
     1021 tgaatcggtt atttatgcag gatttgctgc agcaaggaat atgacgatca aggtagaagg
     1081 aatcgaagtt gcttactcag atcctgcgac agatcctcct gggcaagaaa gacctactcg
     1141 cttgattgat ccgatttaca ctgtgacctc tagtaatcat tcaggaaacc aagatcatac
     1201 tatgtccttt agagcaaatg ctgacggatt attgacaatt aaaaataaag caacaggaga
     1261 ggtgcttaag aacgcaaaaa acttggctat cactgcgaat cgggaatttg attatcagac
     1321 aaaactgatg gaaggaacga acgaatttac tttcagtttt acgccagata aaaactatcc
     1381 gccagaagaa tacgtagaga tgactagtta tgaaacaaaa gagattcttc atacagtgga
     1441 ctaccgtaaa cctaaatatt cgacagttta tgtaacacca gaagggtcag atgcaggaaa
     1501 cggaagcaga caaaatccat tatctctgac acaagcattg agatacgcat atgctgggca
     1561 gacaatcgtc atggcgcctg gaacttattc cttcgaaaga ggattgacga ttccaaaagg
     1621 cgttaacggg acaagagaaa acccgattca gttgattgca gagaaaaatc cagaaaacaa
     1681 gcgtcctgta cttgatttta aaggaactgg aaatgggatg acactgttca gtcactactg
     1741 gggtttacga gggtttgaca ttacaaatag tgcagctatg caaaaaggat tacagatttc
     1801 tggaaatcat aatttgatcg aagagattca tgcttatcgg aatggaaata caggaatcca
     1861 aatcagcggt agctcaaatg atcctttcga agcatggccc gcgcataact tagtcaagaa
     1921 ctgtacatct tttgaaaatg ccgatccagg atttgaagat gccgatggat ttgcggccaa
     1981 actgacaata ggagaaggaa atgtatttga cggttgtatc gcgtatcata atgcagatga
     2041 cggttgggac ctttttgcta aaaatgaaac aggttcgatc ggaaaagtaa tcatcaaaaa
     2101 ctcagttgct tatcgaaacg gctgggtacc aggaattgag ggagaaggta acggtaatgg
     2161 attcaaaatg ggcggttctt ctttaacggg ccctcatgaa ttgatcaata gtttgtcttt
     2221 cgaaaatctt gcgaaaggaa tcgattccaa tagcggaaca gatattatcg tcaatagcag
     2281 tacaagtttt aataacggtt catataatgt ggctctttat acctcttcag caggaaacac
     2341 agactaccat gcttctggta tcttatcttt ccgaacagag aatcttgaga tgagagagca
     2401 gatccgccca ttgaatcaaa acgaagatca aatctaccac tcgttgaact actattggaa
     2461 tgaacgagag aaacaatcag aaaatactct tggacatacc atatcaaaag attggatcga
     2521 gtctctaaat acatgttcga aagaaggtca gcagcctgtt acgcgctatg aaaatggaag
     2581 tatccatgtt catggactga tgcagattaa accggggaat cgtcaaacag aaaaggaaag
     2641 agtggatggt cttccattat ctgtaggtgc tatttttgac ggagaaacct caccgtcttc
     2701 ggaatatcca gagtatgcaa tcgtacaaga accaaatcag acaaaaccaa atgaacaaaa
     2761 taatccgatt attatacaag aagcaaatgg tgtggcggag aatgcagaag gaaatatcat
     2821 tgaactgaag ccttttacag tttttgtgtc aactttcaca ccaaatcaag gaacgcctgt
     2881 ctttgaagat atgcgttatt tcccagaaaa gatccaaatc gttgaaacac agtcaagtgc
     2941 tcaattttct aatcataccg ctattttttc agcaccttca aaaggagaat atacgctaga
     3001 agtcacttat cgttcggaaa tatttgatgg ggaaaaatgg tctgctcata cagaatctgt
     3061 tacccagcaa aaagcgatca ctgtgcgaga aactgccgat gtgcccccgg gaaatgacaa
     3121 accagaaaga gaaaatccag gaaatgaatc accaaatgga aaaccaaatg gaaaaccttc
     3181 tcatccggat aggacagcaa aagaaagtga aaaaaatggc actcaaggac tcccaaaaac
     3241 gggagaattt aataacccgt tgctagcatt agctggagga attttgttaa tcggtgtagt
     3301 tgtttatgtc atgaaacagc gaaaaaaata agtggaattt ctccttttat gatgtaaaac
     3361 aattgactga ggaaagtaaa aatagaagga aatgagacca atagatgtct cacttccttc
     3421 tatcttttta tagatgaggt tctccttgct gcttttgatt ggctgttctt attacttgat
     3481 gcaggacagc tttttcacaa tctctccact aaacggtgtt caatcagctg accctcggta
     3541 ttagctcaac aaacggcaaa atatgaggaa ctatttttcc gtttttttct ctaatactca
     3601 ggtcaggacg ctgatttcac aacctctcga taaacggtgt tcaaaagcca gtttctcggt
     3661 agtaagtcaa atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttactact
     3721 tgaaactaag cagctttttc acaacctcta atacatggta ttccagaagt aacttcttcg
     3781 aaaaataagc cgaaatgtca caaaaatttg agaagcgatt ttcccaaatt ccttcttatt
     3841 tcttgaagct gaccacttct ggcacaacct cttaccagat tcgcactctt ttttctggcg
     3901 ctagatacat agcgtcttgt tccgtgatac caaatgtttc atagaattcg tcaaggtttt
     3961 ttggttgcac gtttgctcgg agtttagcag gggcatggac atcgatttgc agtaaaagtt
     4021 gttggtattc tttttttgct tttgtacgcc aaatcgttgc ccaattgaca aagaattcct
     4081 cgatagaaag atctgattcc gtttttgctg cttccaatgc acagcttaga ccgcctgcat
     4141 cagcaatatt ttccgaaacg gttaattttc cgtttacttt tccgtcagca aaaggcagac
     4201 catcgaattc ttcgatcatt gcttgtgctt tgttttgaaa ttgtgtaaga tcttcttctg
     4261 tccaccagtt gttcaagttg ccatattcgt caaataaagc gccgttatta tcaaatgcat
     4321 gggaaatctc atgagcgata acagctccga ttccaccata attagcagag cttgattgtt
     4381 ctagactgta aaatggtgct tgaaggatcg ctgccggaaa gacgatcaca ttccgaaatg
     4441 gatgataata agcattgacc gtgtcagcac tcatttccca ttctgaacga tccactggtt
     4501 tgttccattt gctgaaacga tcttttaatg tgagtttgct gaaaacaaga gtatttgaaa
     4561 gcaaagtacc accctgatct gcaggtactg ttttgaactg gtcgaataat gctgggatct
     4621 tatcggggta gcctacttgg atacctaagt tatttaattt tatgacggct ttttcttttg
     4681 ttgcatcccc aagccagtca ttttcttcta gacgtttttt ataaacggcg atcatttttt
     4741 tcaccatttg ttctacgtct tttttagcag cttcgccaaa atattttttt ccgtagtaat
     4801 cgccaactac ttgatcgaat tgtccagagg caaggtaata cgctgctttt ttctgaggca
     4861 ttgcttcgtc tgttccagac aatacgcgag aatagattcc gctgacttgg cggaattctt
     4921 ctgatagata accactaaga gaacgtactg ttttaatgat catccagctt ttgaataacg
     4981 gaaaatgagc ttctgtcagg atgtcattca aatgatcgaa gtacactgga tcagttacga
     5041 tcactttatc tggagttgtc ccaatcaaac ccgtgacaag ttttttcaag tccagtttat
     5101 ctgtgtattt aataaatgta tcaaaagatt gcggattata cattttgctg taatctgcgt
     5161 tttcttctgc actttttaca tgaggagcaa tcaatttatc aaattgcact gcttgttccg
     5221 tgattgcttc tgcctgactt ttttctttgc cggcaagctc taataactga acagccatat
     5281 caaagaaaac gcctattagt tgtgcaccgt tgggatggtc tgatgcataa tacgtcttat
     5341 ctggaaggaa taaagaaggc ggataagcaa agaaggcgtt cgtttgcgca ttcttcatat
     5401 ctgcatctac atccagatta aatggcaatg gtaatgtatc caacagccat tctggaaatt
     5461 gcttattcaa ttcgtcgaaa gactgcaatg attcaacttt ttcgattata ggttgaagcg
     5521 gttgagcatg caactcattt cttcggtcat aatcgtttgc cagttgataa tatgcaagga
     5581 aatgtttcat tttttctgaa gacacttttt ctggatgggc aagcagatcg tctgtttctc
     5641 ccatcaaaag ttggtcgatc ccgtcaacta aatcttggaa accgcctgta gctggtttat
     5701 ctgcagggat ttttgctgtt ttttgccatt cttcattgac tgcttcatag aaatcttgtt
     5761 tcaatcgttc tttgttcatt catcgatcgc tccttttttg tttcgtgtgc ccattatctt
     5821 acaaaatgaa ggaattaaca aatagaacat aaaaaattga aaattcgaac aatcaaactg
     5881 atttgttttt ttcaatcctt cacttataat gaaaaaaagg aggcgatccc aactgaatca
     5941 agtagtggat aaacctttat atttcaaagt agataaaaaa attaaaaaat tagccagcac
     6001 tcagcagctg cagtcaagaa aaagtaaatt gcttttttta gctctggttt ttgaagatca
     6061 aagttatgtg attatcgatc aatcaggaca tccggtagaa tattctccag caaaatatac
     6121 ttatcaagag ggtctttctt gtaaaaaatg gaagctgatc aatgaagagc caatcgagtt
     6181 atctagatgg atcaacagaa aagaagatat tcccgttttg atcgaagaaa aaaggtcagg
     6241 aaaggaactc gcaaattgct gggtgggatt gccagaagaa cgttttttga ggtataagaa
     6301 atgggctacc ccctcaggct atctttgtgg tacttacgcg gcagcagttc tccttgccta
     6361 ttatcaagac tatcgtaagg ggtggatgct tccaaatgag attcgcaaga aaaatacggc
     6421 tgattcgttg gttttgacga aggcattgcg tagccagatc caaccactag gacttcctac
     6481 gattcctttt caggttagta cagggatttc tagtttttta aagaagaatg gcaatcatga
     6541 gcgtgcacga gcaactcttc taggttcatg gcagcgagct acaaaacgta tacgtgaagg
     6601 caaacctgtg atgattggta ttttgaaagt acttggcagt acatatggca atcattgggt
     6661 gacagcgtac gcttattttg aaactgaaac aggagaacgt tattacaaag tacacgacaa
     6721 ctggggcgat tatcataaag tcattcctgc cagttggagt aacggaacag tttcacttcc
     6781 ttaagaattt gctgaaatta acagaaaatc ttggaaaaat atgttaaact atcgaagaat
     6841 agagaagttt tggggaggac tacatgagta ataagtatca ggatgacacg ttgaagattt
     6901 ttagtttgaa tggcaaccgt ccattagctg aaaaaatcgc gaaagtgttc ggaacagaat
     6961 taggtaaaag cgtcgtgaag caatttagtg atggcgagat ttccatcaat atcgaagaaa
     7021 gtattcgagg ggatcatgtg tatatcgttc aatcaacgaa tgcacctgtc aatgattact
     7081 atatggaact actgatcatg atcgatgcaa tgaagcgagc aagtgcgaaa acgatcaatg
     7141 tcgtattgcc ttactatggg tatgctaggc aagaccgcac ggcgaaacct catgaaccga
     7201 tcactgcgaa attgatcgct aacttgatcg aagaagcagg ggcaactcga gtactgactt
     7261 tagatctgca cacggttcaa gtacaaggat tctttgatat tcctgtagat aatttattta
     7321 cgatgccgtt gtttgctcat tattaccgtc aattaggtct gacgggagat gacatcgtag
     7381 ttgtttcgcc aaaaaacagc ggtgttcagc gtgcacgtag tctttcagaa tatttgaaca
     7441 gcacattagc catcgtagat catgcagatg atgcttcaac tgagggtggt tatgtgatcg
     7501 gtgatgttca aggaaagaca tgtatcatgg tagatgatat cttgaacaca ggagcaacgc
     7561 ttgcacgagc agcaaatgta ttaaaagaaa acggcgcaaa agaagtatat gcctgtgcgt
     7621 cacatggatt attgtccgat catgcgaaaa gtattttaga caatgcacca atcaaagata
     7681 tttgtatcac tgattctgtt ttcacagaag aaaatcgtca tccagaaaac ttaacaatcg
     7741 ttacatgttc agaattaatg ggtgaagcgg tcaaacgcat ccatgaaaat acaccgatga
     7801 gtccgttatt ccgtctggaa gaaaaagaat tcgaataaac aaaaaaaaga gggaaagaag
     7861 ttgtgacaga agtgagcagc ttcaaggagc tgcgacaaga cttttgtcac agctcctttt
     7921 ttctatgccc atgaaacgtc ctctagacaa aaatgagctc ttctttggtt tgttcgctat
     7981 tttatgctag aatgtgccta gcaataattt ggaagggatg aatgaagtga aaactattta
     8041 tctggatcat gcggcaacta cccccatgca tccacaagta attgaagcga tgacggcaat
     8101 catgcaagac acgtttggaa atccttcaag catacatgga tttggtcgcc acgcgcatga
     8161 aaaattggaa acagcaagac aaatcattgc agacagtctg caagcaaaac ctcacgagat
     8221 catctttaac agtgggggaa cagaagggga taatacagct atcttagaaa cagcgttctc
     8281 gcgtcaaaaa gaaggcagac atctaatcac tacagctatc gaacatcctg ctgttcttaa
     8341 taccatgaaa tatttagaga caaaggggtt tgacgttaca tatctgcctg tagatgaaaa
     8401 agggaatctg tcgatcgaag aggtcaaaaa agcgttacga gaagatacga tcttggtctc
     8461 tgtgatgtac gggaacaatg aaatcggaaa tttgatgcca atcaaagaaa tcggtgaact
     8521 tttagcggat catccagctt atttccatac agatgcggta caagcgtacg gaaaccaaac
     8581 gatccatcca gaagaattag gaatcgattt attaagtatc tctgctcata aaatcaatgg
     8641 gccaaaaggc gtcggtttct tatataaacg agacggagta aatatccctg cattgctttt
     8701 aggcggggag caggaagaaa agcggcgtgc aggtacagaa aatttagcag ggatctgggg
     8761 tatggcaaaa gcagttgaac tgctgtcacc tgaagaacgt gaagcacgta acaacaaata
     8821 taaaggattc cagcaattga ttcttgatac attagaagca aacgaaatcc cttatcaagt
     8881 aaatggtgat ttaacgaata aactccctca tgtgttgaat cttcgcttaa ttggcgaagc
     8941 aaatgatttg ctactgatga agcttgatct taatggggtc gctatttcta ctggttcagc
     9001 ttgtacagca ggaaatatcg agccttcaca cgtattagaa gcaatgtatg gagaagatac
     9061 accgatacta aaagaatcca tccgtatcag ctttggtttg ggaaataccg aagacgacat
     9121 ccagtatttc cttgatcaat tagttcgtgc tgtcaaaaaa taactagtga agtcattctt
     9181 atatgaacag atgtctgaga gaacgccttc attttttagg gaacttaact gaaaaaccta
     9241 gtaaaaatca ggataaactt ttataataaa gagaaactaa ttttatcgag gtgaaaatca
     9301 tggcatttga aacaacagca acggtactgg gcgcaccaat tagctatcgt ttgcatcctg
     9361 atgcaaaaag atatacattg cgcgataatg gatttacaga aacaaacgga ggcaattatc
     9421 aattgatccg accattagat gccacgccgc aaagcaaaga aggattcaaa ttaaaaatca
     9481 ctgtatcaaa agatattcaa acattgaaaa tgtcgatcac tacagcaaat ggattgaaag
     9541 cagtcaatat tttcaaagat ccaaaacaga aaatgagcca agataaattt tattttctga
     9601 tggatgggat gattagccgt gggttattcg aaaaagtcga atgatctgtg gatataaaaa
     9661 gacaactagt tttgaaacgg cctattgtta gatgactaac tttgtaagga cgacgcaaat
     9721 tagttgtctt ttttattttt aaatttaaca catgttgtta tttttaatat tttgctgatt
     9781 tattgtttct aagtaaagaa atttaaattg aaacgattgt tatttaggga aaaaggtatt
     9841 ttttgaaaaa agaatcccgt ataatgattg ttgtgcaaac gctatacaaa atgaaaaaga
     9901 aaagagggat gtttatggaa aatgaacagt cggtcgtttt aacgaattgg aagagaaatt
     9961 atttgttctt tttatcggga cagtttttgt caggaatcac tagtatggtc gtccaatatg
    10021 cgatcatctg gtatttgaca agagaaaccg gttcggcgac aattttgagt tttgctactt
    10081 tactaggaat gattcccatg gttttactaa gcccatttgt aggaccgcta gtggatcgct
    10141 gggataaaaa agctcttttg attgtcacgg atattattgt agcgattttc gcgcttattt
    10201 tagcagtagt cggaacgatt tcagaatcct tcccgatttg gctagttttt gtgtctttgt
    10261 ttatgcgttc ggtcgcacaa acattccaaa tgccaacgat ccagtcgatt atgccgacaa
    10321 tcgttccatc ttctcacata acaaggacga atggacagtt aggaatggtc caatcagcga
    10381 atttcatcat tgcgccagct ctaggtgcgg ctttgttttc agtcgttcct gtcaattacc
    10441 ttattttatt agatgtattg ggggcagttt ttggtgtcgg cttattgatt tttgtgaaga
    10501 tcccaaaagt ttctcctgaa atattggaag ttccgcttac tatttttaaa gatgcaaaat
    10561 ttgggctgca gcaattgatg gataataaag ggttatggta cataacgatc aatggtgcat
    10621 ttgtgatgct gttgttcatg ccagcaatca gtctgtatcc attgatgacg ctagattact
    10681 ttggtggttc cgttggacag gcgggagcag tggaagtagt ctacgcagtc ggaatgctct
    10741 taggcggtgc actgatcagt tttataggaa catggaaaga ccgaatgaaa cctattatta
    10801 tagcctacat cattatgggc ctgacgatcg gtgcaagcgg attggttcca aacgatagtc
    10861 aagggttctt gtactttctt atcttgaatg caggagcagg ttgtgcaacg ccatatttca
    10921 atacactgct gatggcaatg atccagcaga gttatgaatc gaatgtttta ggtcgtgtct
    10981 tagggaactt caactctttg atgaacctgg caggtccaat tggattatta tttgcaggac
    11041 cattagctga tcgtttaggg gtagaaaaaa tgttcttgtt ttccggaatc gggattttat
    11101 tatgcggaat cgttttattt ttaacgtcag ctgcccgaaa ttatgataaa gaattacaga
    11161 aaaaactggt aaaagaacac catgagcaaa aagacgaata gtatggaatg gagttgtgac
    11221 agaagtcttg ttacaactcc atttctgatc ctaatgttcc tgtggcaata agcctactca
    11281 aatgaaaaat atgagtaatt atctttattt gtttaatact tgatataggg cagttttttc
    11341 acgacctatc gtacttcctc tttgcttcat agatttcttt gatttgttgc tcagataacg
    11401 gagtgccaaa tggtagtttt tcctttaaga gttctctttc cggtgcgtct acgcctacat
    11461 tgacatcgtt ctcgataggt acgctgtaat gatggatatg tccatgaaga ttgcggatag
    11521 tgtcgttagg cccgagcaac atcggataat gagtcaagta atattgttga tggtcgaatt
    11581 taacgatggc acctacatca taaaaataaa atttgggact tccgtcagta agcctaggat
    11641 catttttttc taaataacgg aaaaaagcgc gactgtcatg gttccctttg atcaagtgga
    11701 ttgttccttg cagttgagtg accagttcca aaatttctgc atttgctttt tcatattgcg
    11761 gatgcatagc aaggtcgcct agatgataaa catggtcggt ttcttttatt actgcattcc
    11821 agctagtgat caactgttgg ttcatttcat ccacatttgc aaagagccgc ggagcaaagt
    11881 cgttttttcc taataagcgt tcatggaaaa agtgcatatc actgataaag taattcatgt
    11941 atcttttgcc tccctttttt cttagagtaa acaaaatcac ttatctgagc aactcataac
    12001 atctaaaaag aaaaggttgt aaatgagacg tgcagcttta agcgataaga agcaattttg
    12061 aaaaatagtt tttcacaatt tataaaatta ttccttattg ccgaaaggct ggcccttttt
    12121 atgagtatgc caatttttta tatcgaatgc tcaaaagatg agtgattttt tggttgacta
    12181 tcatgtttgt gtactgaata ggtccagtag aacagtattt ttacgacttg ataattaagg
    12241 gatgaaattt tcagaaatat gacgtataat gtaaaatact gaaaagttgc gaccttcaac
    12301 tcgtagatgt ttcagaatct aattgaaatg atggtgatat catgacagac aacagcaaaa
    12361 cacgcgttgt cgtagggatg agcggcggtg tcgactcatc tgtaacagca cttttgttaa
    12421 aggaacaagg atacgacgtg atcggaatat tcatgaaaaa ttgggatgac acggacgaaa
    12481 acggtgtatg cacggccacg gaagattata aggatgtagc gaaagtagct gctcaaatcg
    12541 ggattcctta ttactctgtg aattttgaaa aagaatattg ggatcgtgta tttgaatatt
    12601 tcctagccga ataccgcgca ggacgtacac ctaacccaga cgtgatgtgc aacaaagaaa
    12661 tcaaattcaa agcattcttg gattatgcaa tggatcttgg cgccgattat gtagctactg
    12721 gacattatgc gcaagtcact cgcgatgaaa acggcgtggt ccacatgctt cgtggagtag
    12781 acaataataa agatcaaact tacttcctta gccaattatc acaagaacag ctttcaaaaa
    12841 cgatgttccc tctaggcggc atggaaaaat cagaagtacg ggcaattgct gaacgcgcag
    12901 gccttgcgac tgctaagaaa aaagattcaa cgggaatctg ctttattggt gaaaagaact
    12961 tcaaacaatt tttgagcaac tatctgccag caaaaaaagg aaatatggtc actcttgatg
    13021 gtgaagtcaa aggacaacat gctggcttga tgtattatac gattggtcaa cgtcaaggac
    13081 tagggatcgg tggcggagga gattcgcaag aaccctggtt tgttgtagga aaagatctag
    13141 cgacaaacac attatatgtg gggcaagggt tccatcatcc tgcattgtat gccacaagct
    13201 tggatgcaag tgaaatccac tttacgacga atgaaccaat gccaaaagaa ttcaagtgta
    13261 cagcgaaatt ccgttatcgt cagcaagacg ttcctgtgac agttcgctta ttggatgata
    13321 atcgtgcaga agtcgtattc gacgaacctg tacgtgcaat cactcctgga caagcagttg
    13381 tattttatga tggaatggaa tgtctgggtg gtggtttgat cgaccacgca tatcaagaaa
    13441 caaaagtttt acaatacgtt taagcgatat taaatgaatc aaaacagcta tggctaacaa
    13501 aagaaacacc ttttcttttg gatagcatag ctgttttttt gcaataaaat taatttttat
    13561 tcattttata cttgaaatac ttgtgtaaaa agttcataat atccaataat atgaataaag
    13621 ttgctttttg ttaagcaaga gcagttgcaa aatagaaggg aatcttttgc aagtgatttg
    13681 ttcaatttta taagtgaggg tgatggaaat ggaaaagaaa tcgacgggta ttagcaaatg
    13741 gggactggtt gctctggttg tcagttcgtc aatcggaagc ggtgtgtttg ggatcacaag
    13801 tgatctggct agttctgcag ctccagggcc agcgatcctt tcatggataa tcgttgggat
    13861 tggtatttta gctttggtat tatcgctgaa tcatttagga gaaaagcgtc cagatctaga
    13921 tggtggtatt ttcggttacg cggaagcttc atttggtaaa ttaggtggct tcatcagtgg
    13981 ttggggttac tggttgtctg cgtggctggg aaatgttgct tttgcaacga tgctgatgag
    14041 cgccatggga gaatttttcc cgatattcaa aggtggacaa aatgttcctt ctattttatt
    14101 tgcaagtatt tttatttggg gtctgacttt attagtaaac aatggagttg aaggagcgag
    14161 cgtcatcaat acaatcgtga cgatctgtaa attagtgcca ttattcctat ttttaatatt
    14221 tgtatttatc gcatttaaag tgaatatatt cactgctgat ttttggggta acgtatctga
    14281 taatctagtg aatggaacag ggcagcaagg aacgatctgg ttgcagatta aaggttgttt
    14341 gatggttatg atgtgggtct ttgtagggat cgaaggcgca agtgttttag ccaaccgcgc
    14401 gaagaaacgt tcagaagccc agcaagcatc gatcattggt ctgctttgtt tattgtttat
    14461 ctatatcctt gcttcgatct tgccatacgg cgtcttatct caagaagagt tagcaactat
    14521 ttcacaacct gctatggcaa atattttaaa aggaatcgtg ggaaattggg gagcagcgct
    14581 gatcaatatc ggcttgatta tttcgatcat tggttcttgg ttgtcttgga cgatgctccc
    14641 agcagaaaca acgatgttga tggctcgaga cggtctgttg ccgaaaggtt ggggaaaaac
    14701 gaataaaaaa aatgccccaa cttattcgtt agttgttact gcaatcttga ctaatctatt
    14761 cttattgact ttcttagtta ctgattatgc ctatcaattt gcctattctt tatgtacagc
    14821 ggcaattttg atttgttatc tacttgtcgg gatctatcaa gtccagtttt ctttaaaaga
    14881 aggaatcaaa aaacaagtag tgatcggtgg gattgctgta gtttttgaat tgatgggcat
    14941 cattttagca ggattttctt atgtcctttt atgtagtatt gcctatcttc ctggtttttg
    15001 cttctactgg atcgcttgtc gtgaaaccaa acatcaaatt acgggaaaag aaaaagtaat
    15061 gattgggctg atttgtgtgg ccgcactgct ttcaatcggg ttgcttgctg taggctggat
    15121 caagatataa gggggaaaga aacatgaaaa tcgcaaattt cggtgtagaa gaatggctaa
    15181 ataaatggga aaagaaagcg gtatatgata ttgcacaaag ctcgatcgaa gcattgacat
    15241 tagacgaact cgttggctta gatgggacta gcgtaagtaa atttttcgaa aaacgcaaaa
    15301 acgaaccatt ggactatggc tggatcgaag gatcagatgt tttcaaacaa gaagtcgctt
    15361 ctctttatca aaccgtcgat ccggaaaaca tcttacaaac gaatggagcg actggtgcta
    15421 atcttttagc tctatacgcg ttagtggaag caggagacca tgttgtttcg atgcttccta
    15481 cttatcagca attatatgac atccccaaat ctttaggtgc cgctgttgac tttgttcatt
    15541 tgaaggaaga agaagattgg cagtttgatt tagaacagct agaagcctta gtcaaacctg
    15601 aaacaaaaat catctgtttg aatagtgcaa acaatcctac aggcacattg ctagatcgga
    15661 aaactttaga aaaaatcgca gaaattgctc gtacagtaga tgcatatgta ttgatagatg
    15721 aagtatatgc gcctttaacg gataaaggtg aattcttgtc gatcgtagat gtctatgaaa
    15781 aaggtatagc aaccaattcc ctatccaaaa cctactccat tccaggtatt cgtattggct
    15841 ggacagctac tggacctgag ttggcagaag tttttcgaaa gtatcgagac tacacgatga
    15901 tttgcggcgg agtcctgtca gatgatcttg ctgtacatgc attgaagaat aaagaaaaaa
    15961 tattggcgcg aaacaaaaag atcattacag agaaccttgt gattttaaaa caatgggtag
    16021 ccaatgaacc gaaagttgaa cttgtcgctc ccaattacgt ctctacttct ttcattaaat
    16081 tagccatcga agaagatgac cagacatttt gtatcaattt attagaagaa acaggagtac
    16141 tattagtacc aggaagtgcc tttgatttac cgaagcatgc ccgactaggt tactgttgta
    16201 aaaaagaaac cttggaaaag ggattattcc tcttatctga gttcttgaaa aaacaggcct
    16261 aaacaaaaac actgcaagga cagtctgtga caactgttca agcagtgttt ttcatttacg
    16321 cttattgtct atgagaagga gagtgccctg aagctatgga gactcctgaa gctttttctt
    16381 ctgcagtttc ttgctgatgg atctcttgtg tattgtgtcg tacaagcaag aagttgacca
    16441 aaacaagaag agaagtcgaa agaaagacgc cgcgataacc aaatgcactg gaaacactgg
    16501 agccgatcat aggccctact acgttaccag ttgcttggaa agattggtta taactgaaga
    16561 tccgtccagc agcttgatga ggcgaatatt ttgtgatcaa tgcttggaca gcagggagca
    16621 gacaagcatc agagatacca atcaaaaaac gtaaggcagc taattgccaa acattcgtga
    16681 cgaatgccat tgggatataa accagaatcg caaaacccaa accaatcgca agtattcttt
    16741 cactcccaat cttgtcacct aagcgaccaa atcttggagc ggctatcaaa gtggcaatcc
    16801 caggtatcga agcaatgatt ccgctgatca acgtaacatt cccatgtcca tgcagcaatt
    16861 ggcgaatgta cagactgatg attggactaa tggaattgtt ggatgcttgg atgatcatcg
    16921 ttgtgacgaa cataccgatg acgacgtgtg gatatttcaa gtctttgaaa atctgtttgg
    16981 cagaaaccat gttttctttt ttcactggga caaatttttc atggacgaaa aacagactca
    17041 acaagaagac gaaaaataag atgatccctg taatgaaaaa ggtagggcga tagccaaacg
    17101 ctgaagcaga gatcccgcca agaagcggtc caagtaatgt tcctgtaaca gaaccagttg
    17161 ctaaggttcc aagaacttga ccactttttt cttttggcgt cccagttgcg acaagggcag
    17221 tggcattact gatataacca gaaaaaatcc cttgaagcag acgcaatgcg actaattgat
    17281 acacactagt cacgcaaccc atcaaagaaa tgacgattgc cattcctaat gaagcacgca
    17341 acagcatcaa ttttcttcct ttttggtcgg ctaattttcc ccaccaagga gagataagcg
    17401 tagtcaccaa gaaagtagat gaaaaagtca atccgctcca aaagttcaat tgtgacgtac
    17461 tataattacc taacgtatct atgtaaagtg acataaaagg catgactaaa ctaaatccga
    17521 taccagccat aaatgttcca aaccataaga caaacaagtt ttgttcccaa gtttctcttt
    17581 tcctaaataa tttttccttt attcttgcag ccaattagac tccttctttc tgaaaatttt
    17641 catctacagc tctttattat aaactacaat atgtagttta tcaatgaaag tctctttttt
    17701 ctctttcttt ttcgtggtaa actgaaagag tgaaggattt attttatgag aggaaacatg
    17761 gaaatgaaaa aaagaaatct gtctcaagag aaaatcatcg actgctttcg agaactagca
    17821 gaagagatgg atgttcagca aataaccttt cagcatttag ccaaacgctt ggatatcaag
    17881 gctccctcgc tgtataacca tttcaaaaat attcgtgatg tcaaaacagc gctaacagcg
    17941 aaacttctgc aggaacttaa tgaccagctg cgacgggctc ttgtgggaaa aagtggtcag
    18001 gaagcactgc agatctatgc agaaacttat cgatcctttg ctttttcaaa tcaagcagtg
    18061 tatgagttac tgatcagtgt accgcatacc aatgaagctg ttttattaga tggaatccat
    18121 gaaacgaatc agatcatttt gcagatattt gaggcttttc aattaacgaa agaagaaaaa
    18181 gttcatcgaa gcagggaact tcgtagtatg atccacggtt atctttcctt acgtttttta
    18241 ggatacttta caaaagaacc aacaatcgat ccagaagaca gctatttttg gatgatcaat
    18301 aactttatcg ctactttgcc ttaaacaatc agattcattt gtaacaattt taagatgttc
    18361 attaaatgac ccttttataa gagaaaattc ttgcaacttt cttgaaaagt gactaagatg
    18421 agtctagaaa agctgaaaga aggagcacga gtatgtatca agtaattacc atgttcgggg
    18481 acaatgagcc ttggtggttt tttgaagact gggaagaaga tattgaagaa gaagaaacat
    18541 tcgagtcttt cgaagaagca cgacaagcgt acgaaaaaca atggaacgaa atccatcaaa
    18601 attatgaata tatcaatgca aaaagtaact ttctcagcgc cttttggaat gacggagatg
    18661 agcgctggtg tgaagagtgt gacgatgatt tacagcaata caagggactt gcgttgttga
    18721 aaaatcatca gcccatcaca gtagaaagca gaaaggaatt ttatgaaaca actaattcta
    18781 gcggaaaaac caagcgttgc gaaagaccta agcaaggtgc ttggtgctaa tcaaaagcac
    18841 aaaaattatt atgaaggacc caaggtgatc gttacttggg cccttggtca tttattagga
    18901 ttgaaaatgc cggaagatct gaataaggaa tggcagagct ggcaaatgga cacgttgcca
    18961 atgatcccta aaaaactagg aatcaaacca cttcctaaaa caggacatca attaaaagca
    19021 atcaagcagc tggctcagcg aaaagatgtc tcagaagcag tcattgcgac agatgccgga
    19081 agagaagggg aacttgttgc tcgctggatc ttagagtatg tgcatttcaa taaaccagtg
    19141 aagcggttat ggatctcttc tcaaacagat aaagcaatca aaactggatt caagcaacta
    19201 agacctgcga aagaatatga tgcattgtat gaatctgctc ttgcccgggc aaaagcggac
    19261 tggctggtag gattgaatgt gaccagagcc ttaactgtga agtacgcgga taatttatca
    19321 gcaggccgtg tgcagacacc gacactagca ttagtgagag accaagaaaa gaaaatcgag
    19381 cagttccgtc cacaaacgta tttcacgatc acgttgcaag ctggaaaaga acaagccaaa
    19441 atgcttcaaa aaaatcaatt tgctttaaaa acacaagaag aagcacaaaa acttgttcaa
    19501 ttgatcagta caagcaaagg aactgtcaca gaagtccaag aaaaattaaa aacggaatct
    19561 gcgccattgc cttatgattt aacagagatc caaagagaag ccaatcaacg ctatggctat
    19621 tctgcaaaga aaacattaag tcttgtacaa agtttgtatg agacccataa gatcgttact
    19681 tatccgcgta cagacagcaa atatctgaca aatgacatga aaagtacgat gaaagaacga
    19741 ttgcaagcag tcagcgactt tgctcctgag gtcaagcagt atttgaaaaa cggagccatc
    19801 gtccaacaga caaaagtatt ccaagacagt aaagtgaccg atcaccatgc acttttgcca
    19861 actgaaaaca gagcacgtta tgaaaaatta agtaatgaag agcgaaaaat ctatcaaatg
    19921 attgtctctc gttttttagg actgtttgct caaccccata aagtcagcca aacaaaagtt
    19981 acggttgaat ttggtaaaga acgattcatt tttcggcaaa atcgtgtcgt ccaagcagga
    20041 tggaaaggcg aaactgaaac tgagacggaa acagtcaaat gggaaaaagg aatgagaatc
    20101 aatcccgatt tcgccatcaa aaaagaacta tctgcaccgc ctaaaccatt gtcagaagca
    20161 agcttgcttg gtcaaatgga aaaatacagt ctgggaaccc cagctacgcg tgcagagatt
    20221 atcgagaaac taatcaaatc tgaactgatg gaacgtaccc ctcaaggact gcaagtatct
    20281 cctaaaggta agcaattatt agtattggta aatccctctt tagtaacacc agatttgaca
    20341 gaaagctggg aaaaagacct tgaagctatt gctgcaggaa agaaacaagc aggaaccttc
    20401 ttgaatggaa tcgaaaaaga aacaaaacgc ttggtgaatg agatcaaatc aagcaaacaa
    20461 gaatatcaag atttctcgat tacccaaaag aaatgcccag aatgtggggc caatctgcgc
    20521 gagaaaaaca cacgtgacgg caaaatctat gtctgcacca attcagattg caactaccgc
    20581 agaagaaaag atccaaaagt ctctaatcat cgatgcccgc aatgtcaccg gaaaatggaa
    20641 atcatcgaag gtaaaaacgg tgcttatttc cgttgtaaat acgatggaac gacagaaaaa
    20701 ttgcttgata aaaaagagca gaagaagaaa atgaccaaac atgaagaacg acgcttgctg
    20761 aaaaaatatg cacaagcaga agaaccagaa gaaagcccat tagcagcagc actaaaagca
    20821 gcaatgaaga agtaataaga tatgaaagag gttgtgatag aagtggtcag cttcaagaaa
    20881 taagaaggaa tttacgaaaa ttgcttctca aatttttgtg aaatttcggc ttatttccga
    20941 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
    21001 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
    21061 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
    21121 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
    21181 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
    21241 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
    21301 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
    21361 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
    21421 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
    21481 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
    21541 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
    21601 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
    21661 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
    21721 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
    21781 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
    21841 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
    21901 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
    21961 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
    22021 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
    22081 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
    22141 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
    22201 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
    22261 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
    22321 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
    22381 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
    22441 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
    22501 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
    22561 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
    22621 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
    22681 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
    22741 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
    22801 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
    22861 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
    22921 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
    22981 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
    23041 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
    23101 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
    23161 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
    23221 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
    23281 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
    23341 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
    23401 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
    23461 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
    23521 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
    23581 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
    23641 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
    23701 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
    23761 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
    23821 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
    23881 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa ta
//
LOCUS       DAZKOU010000042        23776 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000042 DAZKOU010000000
VERSION     DAZKOU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.042"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG827_001569"
     CDS             complement(164..388)
                     /locus_tag="ACG827_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0483713.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG827_001570"
     CDS             complement(586..2166)
                     /locus_tag="ACG827_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0483714.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG827_001571"
     CDS             complement(2567..3943)
                     /locus_tag="ACG827_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0483715.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG827_001572"
     CDS             complement(4071..5159)
                     /locus_tag="ACG827_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0483716.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG827_001573"
     CDS             5331..5765
                     /locus_tag="ACG827_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0483717.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG827_001574"
     CDS             complement(5843..6376)
                     /locus_tag="ACG827_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0483718.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG827_001575"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG827_001575"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0483719.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG827_001576"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG827_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0483720.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG827_001577"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG827_001577"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0483721.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG827_001578"
     CDS             complement(10101..10679)
                     /locus_tag="ACG827_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0483722.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG827_001579"
     CDS             11001..11471
                     /locus_tag="ACG827_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0483723.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG827_001580"
     CDS             complement(11579..12805)
                     /locus_tag="ACG827_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0483724.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACG827_001581"
     CDS             complement(13559..13759)
                     /locus_tag="ACG827_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0483725.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACG827_001582"
     CDS             complement(13893..14501)
                     /locus_tag="ACG827_001582"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0483726.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACG827_001583"
     CDS             complement(14586..15056)
                     /locus_tag="ACG827_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0483727.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACG827_001584"
     CDS             complement(15104..16018)
                     /locus_tag="ACG827_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0483728.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACG827_001585"
     CDS             16164..16967
                     /locus_tag="ACG827_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0483729.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACG827_001586"
     CDS             17145..18092
                     /locus_tag="ACG827_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0483730.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="ACG827_001587"
     CDS             complement(18160..18453)
                     /locus_tag="ACG827_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0483731.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="ACG827_001588"
     CDS             complement(18481..19146)
                     /locus_tag="ACG827_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0483732.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="ACG827_001589"
     CDS             complement(19286..19918)
                     /locus_tag="ACG827_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0483733.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG827_001590"
     CDS             complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG827_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0483734.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG827_001591"
     CDS             complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG827_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0483735.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG827_001592"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG827_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0483736.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG827_001593"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG827_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0483737.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         7053 a         4830 c         3826 g         8067 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatg
//
LOCUS       DAZKOU010000043        23599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000043 DAZKOU010000000
VERSION     DAZKOU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.043"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG827_001594"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG827_001594"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0483738.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACG827_001595"
     CDS             complement(1174..1869)
                     /locus_tag="ACG827_001595"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0483739.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACG827_001596"
     CDS             complement(1882..2598)
                     /locus_tag="ACG827_001596"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0483740.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACG827_001597"
     CDS             2928..3536
                     /locus_tag="ACG827_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0483741.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACG827_001598"
     CDS             3861..4184
                     /locus_tag="ACG827_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0483742.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACG827_001599"
     CDS             4366..5736
                     /locus_tag="ACG827_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0483743.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACG827_001600"
     CDS             complement(5866..7167)
                     /locus_tag="ACG827_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0483744.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACG827_001601"
     CDS             complement(7521..9512)
                     /locus_tag="ACG827_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0483745.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACG827_001602"
     CDS             complement(9656..9922)
                     /locus_tag="ACG827_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0483746.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACG827_001603"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACG827_001603"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0483747.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACG827_001604"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACG827_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0483748.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACG827_001605"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACG827_001605"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0483749.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACG827_001606"
     CDS             complement(14082..14603)
                     /locus_tag="ACG827_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0483750.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACG827_001607"
     CDS             complement(14606..15448)
                     /locus_tag="ACG827_001607"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0483751.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACG827_001608"
     CDS             complement(15441..16166)
                     /locus_tag="ACG827_001608"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0483752.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACG827_001609"
     CDS             16342..16638
                     /locus_tag="ACG827_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483753.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACG827_001610"
     CDS             17206..18798
                     /locus_tag="ACG827_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0483754.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACG827_001611"
     CDS             18811..19551
                     /locus_tag="ACG827_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0483755.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19719..21068
                     /locus_tag="ACG827_001612"
     CDS             19719..21068
                     /locus_tag="ACG827_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0483756.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21196..21786)
                     /locus_tag="ACG827_001613"
     CDS             complement(21196..21786)
                     /locus_tag="ACG827_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0483757.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21933..23285
                     /gene="brnQ"
                     /locus_tag="ACG827_001614"
     CDS             21933..23285
                     /gene="brnQ"
                     /locus_tag="ACG827_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0483758.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6748 a         4758 c         4498 g         7595 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagtcatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaaata aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtatctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caatcaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacaaactt ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcca tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgaacaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaacagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatta ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaaaattt tggaatctta gttgtgtttt tcagaaaact cttttaatat
    19681 ggaagtaatt aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc
    19741 agcgtggact gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg
    19801 ggttgttttt aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct
    19861 atcttattac tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat
    19921 caaacaccaa taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg
    19981 ctttcatcac tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa
    20041 cagccatcgg aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac
    20101 tgattgcatt aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt
    20161 tagagttttg gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag
    20221 cttatatgat cctcacccac tatacgacag atattggcac agccagtatc acgaatctat
    20281 ggtctcatgg cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg
    20341 taacctttgc ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc
    20401 ctgaaaaagt gttgccggaa gctatcaata atattcctat caggatcatc ttattttact
    20461 taggatctct ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca
    20521 gtccatttgt agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt
    20581 tagtcgtatt aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta
    20641 tgcttcgttc tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac
    20701 atcatgtgcc agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct
    20761 tgaattacgt gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt
    20821 tcttgtttat ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg
    20881 gcaaaaaagc tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc
    20941 tttacatggc ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat
    21001 tactaacacc tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta
    21061 agaagtaaga aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac
    21121 gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tcttggagtt aaaagcttct
    21181 gtcccagcct cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt
    21241 ataggaaaaa atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac
    21301 aaaaaagaca ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc
    21361 ctttgacaaa actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat
    21421 caagatacca aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca
    21481 aaaagcttca gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa
    21541 gagcaaaaag atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat
    21601 acgatgatgg ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa
    21661 tgtatttcgg atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa
    21721 tacaagaact ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt
    21781 tgtcacgatg attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa
    21841 ataaccgtcg ttttatatag gaaattttct gaaaattgtt gcatattttt cgaaattttg
    21901 ttacttttaa aaaaaccgta aaggagttat gtatgacaaa aaaattatca tttaaagact
    21961 atctttttat aggctccatg cttttcggtc tattttttgg tgctggaaat cttatctttc
    22021 ccgttcattt gggtcaagaa gcaggcgctg ctaccttttg ggcgaatcta ggtttcttgg
    22081 ttaccggtat cggtcttccc ttcttagggg tgattgccat tggtgtatcg aaaagctcag
    22141 gtgtatatga attagcaaaa cggataaatc aaacatatgc gatggttttt accatcttgc
    22201 tttacttagt tatcggaccg ttttttgctt tacctcgcct tgcaaccaca tctttcgaga
    22261 ttggtttagc accatttatc gatcctagca gacagacgct atatctagca attttcagta
    22321 ttgtattttt tgtgctggct tggtggtttt caagaaaacc gacaaagata ttagattatg
    22381 ttggaaagtt cctgaatcct gcatttttgg ttttattagg tgctttgctt ctccttgctt
    22441 ttttgaatcc actaggttct gtaaatcaag cgcctattca gccaaattat caagagcatg
    22501 cttttttcac agggtttact caaggatata acacattaga tgcgcttgct gctttagcat
    22561 ttgggatcat cattgtcaca acaatccaaa acatgggtgt aacaaaacct gctgaaatcg
    22621 ctaaagacac gatcaaatct ggtgcgatca gcattatatt gatgggaatc atttatactt
    22681 tgctcgctta tttaggtgct atgagcttag gtagctttgc tctaagtgaa aatggcggga
    22741 tcacgctggc tcagattgct gatcattatt taggtactta tggcagtatc ttattagcat
    22801 ttatcgttat attagcttgc ttgaagaccg gtattggttt gattacagct tttgcagaaa
    22861 cattcacaga tttgtttccg caaagaaatt acgcattctt tgttgcactt gctagtatcc
    22921 ttccttgttt agcggcaaac gtaggcttga cgaatatcat tcaattttcg ttgcctgttt
    22981 tgatgttcat ctatccatta gcaatgacac tgattttact agtgttagtt ggtcctttgt
    23041 ttaagcagcg tacggctgtt tatcgaatga ctacttattt tacattgatt gcttcgattt
    23101 ttgatgggtt gaacgcttgc ccagaaacta tcaaacaaac acctattgtt caaaatcttt
    23161 tatatgttgc ggaaagctac ttgccgtttt tcaaattggg gatggggtgg atcgtaccag
    23221 cagttatcgg ttttgttatc ggactgattt ggagtttcgc taagaaagaa gaagtagcag
    23281 attgataaaa gaggttgtga aagagacgtt cagccttatt gccgataggc tgtctcttga
    23341 acactgttta ttgagaggtc gtgaaaaagc cgctcagttt cgagtattaa gaaatatttt
    23401 ttgaaaatgg cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt
    23461 gaacaccgtt tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa
    23521 gtagctccat atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac
    23581 aacatttctt ttataatat
//
LOCUS       DAZKOU010000044        22719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000044 DAZKOU010000000
VERSION     DAZKOU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.044"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1942)
                     /locus_tag="ACG827_001615"
     CDS             complement(11..1942)
                     /locus_tag="ACG827_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0483759.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG827_001616"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG827_001616"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0483760.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="ACG827_001617"
     CDS             complement(2871..3623)
                     /locus_tag="ACG827_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0483761.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="ACG827_001618"
     CDS             complement(3782..3991)
                     /locus_tag="ACG827_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483762.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="ACG827_001619"
     CDS             complement(4002..5330)
                     /locus_tag="ACG827_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483763.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="ACG827_001620"
     CDS             5466..5621
                     /locus_tag="ACG827_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0483764.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG827_001621"
     CDS             complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG827_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0483765.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG827_001622"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG827_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0483766.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG827_001623"
     CDS             complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG827_001623"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0483767.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="ACG827_001624"
     CDS             complement(9305..10237)
                     /locus_tag="ACG827_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483768.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="ACG827_001625"
     CDS             complement(10394..11053)
                     /locus_tag="ACG827_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0483769.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG827_001626"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG827_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0483770.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="ACG827_001627"
     CDS             complement(12094..12384)
                     /locus_tag="ACG827_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483771.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="ACG827_001628"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="ACG827_001628"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0483772.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG827_001629"
     CDS             complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG827_001629"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0483773.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16049..17218)
                     /locus_tag="ACG827_001630"
     CDS             complement(16049..17218)
                     /locus_tag="ACG827_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0483774.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG827_001631"
     CDS             complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG827_001631"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0483775.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG827_001632"
     CDS             complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG827_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0483776.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22182..22703
                     /locus_tag="ACG827_001633"
     CDS             22182..22703
                     /locus_tag="ACG827_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0483777.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6326 a         4932 c         4041 g         7420 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc tttgactact
    14701 gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc tgccatcact
    14761 cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga tcccatttca
    14821 gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc gtttttatca
    14881 cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc aagctctgat
    14941 aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag catcccatct
    15001 ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc aaagttataa
    15061 tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc
    15121 aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt cgtaacgatc
    15181 gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa catgatttta
    15241 tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc atcgccatcg
    15301 aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg ttctggatgg
    15361 gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat tgtataaaaa
    15421 tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc gtttgctgca
    15481 tcgacacaaa tggtcaaacc ggaaaggtca ccgttaatcg tttgtacaag gaattgagaa
    15541 tatttcaata agccttctgg aaattcttct acagttccca atccttcagc tgatggacga
    15601 ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg gtcatctact
    15661 agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg attatgagaa
    15721 gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc aacacctggt
    15781 gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc tgaaatcagc
    15841 gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac gcgtgggcgt
    15901 tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag cttaaatgca
    15961 agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc aaaatattta
    16021 cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt ctgctcattt
    16081 gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag
    16141 attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca gcagatgaaa
    16201 tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca gagctactgc
    16261 ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg atacttaacc
    16321 cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct gagacattga
    16381 tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg cttacttctg
    16441 cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt gttccttgct
    16501 gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc actgttactt
    16561 gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc tgtattggga
    16621 cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct actactcgtt
    16681 tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga ctaacggaag
    16741 tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt tccacatcga
    16801 aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg atctttgctg
    16861 tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct tcgcccagct
    16921 tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct gcattcaact
    16981 gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca tggatatagt
    17041 agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga ttatacgctt
    17101 catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac agcgcaatac
    17161 tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt gagatcattt
    17221 gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt tcttgttttt
    17281 tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt gctgggataa
    17341 gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt cagagacaac
    17401 gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc gagtcccgaa
    17461 ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta ctgcgatctt
    17521 tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa agatattgat
    17581 cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat actcatctaa
    17641 tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca tatattggat
    17701 ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt ttgattgttt
    17761 gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct gaaaaatcac
    17821 gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca aggtgtgcag
    17881 accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt tcagaagctg
    17941 aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc agacaaccag
    18001 aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa acatttgctg
    18061 ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc tctcttctct
    18121 tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat atgctcgtat
    18181 ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt tatttttctc
    18241 tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg ttatttttcc
    18301 tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc agctcttcta
    18361 gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt
    18421 acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt
    18481 gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa taaagctttt
    18541 gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct catgaaatca
    18601 ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt gtttttttcg
    18661 accattgtag ggttatatcg atataatgac caataaccag aagaaacagc ttcttttgct
    18721 tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg cgtataggca
    18781 ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat cgtttgcatt
    18841 tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat cgccatcatt
    18901 cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat tgccgaagct
    18961 ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc caaaacaagg
    19021 atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa
    19081 gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc ctttctcttg
    19141 tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt
    19201 gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc ttccatcaat
    19261 tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc caaagcctcg
    19321 cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc ttccaataaa
    19381 gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga agcaggagaa
    19441 gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg atcaccgaac
    19501 atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat
    19561 tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt gactggattt
    19621 gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt ctgttcttca
    19681 taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca cagaccacaa
    19741 cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc gcctcgcatt
    19801 tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc taaaaatgga
    19861 cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca gcgatcacta
    19921 atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt tcctaaaggc
    19981 atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg atttccttcc
    20041 tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt attgattttt
    20101 tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag cagttcggtc
    20161 gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg tccatagctt
    20221 ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat gtcagctaat
    20281 ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc
    20341 aatctcatgg cattgatcgt atagaatcta atgttatttt cagccaagta acgttttaat
    20401 tttaagggca gatgcgtttc taattgttcg tcgctccaaa gagtattcaa taaaaaaatg
    20461 ccacctggct tcaatccttt gaccaagtca tagctatgca ggtaagctgg tgtgtgacag
    20521 gcaacaaggt ccgcatgctc gacaagatac gctgaacgta tcggcgtatc tccaaaacga
    20581 agatgagaga cagtcagacc gccagatttt ttcgaatcat aataaaaata gccttgcgca
    20641 tatttatcgg tgtgatctcc aataatcttg atagctgatt tatttgctcc aactgttccg
    20701 tcagatccaa atccccaaaa cttggcctgg aatgtttgcg gttctgtcaa atccagactt
    20761 tcctttggtt ccaaagattg ataagtcaca tcatccacga tccctatggt aaaccgtttt
    20821 ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg ctgagatttg gtcgggagtc
    20881 acatctttag accccaatcc gtagcgacca ccaataacag ccgggcgaag ttcagaatca
    20941 tataacgcac tttgaacgtc taataagaga ggttctccac ctgcccctgg ttctttactt
    21001 cgatcaagaa cagcaattgt ttttactgta tgaggcaact tttgcacgaa tgtttccact
    21061 ggaaaaggac gataaagatg gatattcaaa aaacctgttt tccgtccgtt tgcattcaag
    21121 taatcaatcg tttgttcaat cgtctgtgca actgatccca tagaaacaat gacttcttcc
    21181 gcatcgtctg ctccgtaata agtcaccaag tcataagaag ttcctcgtaa tcggttgatc
    21241 tctttcatat atttttgaac gatttcaggt aatttatcat aataggggtt gatcgtttcc
    21301 cgttgttgaa aatggatgtc tggattctgg ttcgtaccac taacagtcgg atgattggga
    21361 ttcatccctc gtttacgaaa tgaagctaat ttctcctgat tgatcagagg agctaattct
    21421 tcatattcca aaacttcgat tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa
    21481 ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg caacaggtgc taaatccatc
    21541 acttcttgta cactgctttc agctaacata gcaaatcctg tttgtcttgc agccatcacg
    21601 tctccgtgat ctccgaaaat attcaacgca tttgtcgtca ctgcccgact agcaacgtgg
    21661 aaaacacttg gcagtagttc cccagcaatc ttgtacatat tcggtatcat caaaagcagc
    21721 ccttgtgaag cggtatatgt agttgtcagt gcaccagttt tcaatgatcc atggaccaca
    21781 ccagctgctc ctgcttcgga ttgcatttct actactttta ctggttgacc gaatacgttt
    21841 tcttttcctg ctgctgacca ttgatctact aattcagcca tggtcgaact gggtgttatt
    21901 ggatatattg cagcaatctc ggtaaatgca taagaaatat atgcggctgc cgtattgcca
    21961 tccattgttt tcatttttcg catgattcat catccttgtt catattctct cttattatct
    22021 gttccattat aacgtaaaac atgccatttt tttcttttat ttataaaaat attttacatg
    22081 tatctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    22141 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    22201 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    22261 aaatggtcga attcatcaac ttagcttgtc cgtattgccg acaatggttt gaagaatctt
    22321 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    22381 ataaagaaaa agaaagctta cagcgaggga acgtgatgca ccggtatctc acaatcagcg
    22441 atggccaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    22501 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    22561 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    22621 ttccaactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    22681 aagaattgat ccaaacaaaa taaaaaaata aggagcgtg
//
LOCUS       DAZKOU010000045        22247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000045 DAZKOU010000000
VERSION     DAZKOU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.045"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACG827_001634"
     CDS             5..403
                     /locus_tag="ACG827_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0483778.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="ACG827_001635"
     CDS             387..1082
                     /locus_tag="ACG827_001635"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0483779.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1676..2563)
                     /locus_tag="ACG827_001636"
     CDS             complement(1676..2563)
                     /locus_tag="ACG827_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0483780.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2748..3587
                     /locus_tag="ACG827_001637"
     CDS             2748..3587
                     /locus_tag="ACG827_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0483781.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3783..4703
                     /gene="sgrA"
                     /locus_tag="ACG827_001638"
     CDS             3783..4703
                     /gene="sgrA"
                     /locus_tag="ACG827_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0483782.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSMSSESSTGSTSSESSTGKVN
                     NELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSR
                     TLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5001..5357
                     /locus_tag="ACG827_001639"
     CDS             5001..5357
                     /locus_tag="ACG827_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483783.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5402..6517
                     /locus_tag="ACG827_001640"
     CDS             5402..6517
                     /locus_tag="ACG827_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483784.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6543..6983)
                     /locus_tag="ACG827_001641"
     CDS             complement(6543..6983)
                     /locus_tag="ACG827_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0483785.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7143..7649
                     /locus_tag="ACG827_001642"
     CDS             7143..7649
                     /locus_tag="ACG827_001642"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0483786.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7817..9730
                     /locus_tag="ACG827_001643"
     CDS             7817..9730
                     /locus_tag="ACG827_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0483787.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9735..10736
                     /locus_tag="ACG827_001644"
     CDS             9735..10736
                     /locus_tag="ACG827_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0483788.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10938..11144)
                     /locus_tag="ACG827_001645"
     CDS             complement(10938..11144)
                     /locus_tag="ACG827_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0483789.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11790..12638)
                     /locus_tag="ACG827_001646"
     CDS             complement(11790..12638)
                     /locus_tag="ACG827_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0483790.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12802..13704
                     /gene="gnd"
                     /locus_tag="ACG827_001647"
     CDS             12802..13704
                     /gene="gnd"
                     /locus_tag="ACG827_001647"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0483791.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13706..14707
                     /locus_tag="ACG827_001648"
     CDS             13706..14707
                     /locus_tag="ACG827_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0483792.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14704..14988
                     /locus_tag="ACG827_001649"
     CDS             14704..14988
                     /locus_tag="ACG827_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0483793.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15021..15818
                     /locus_tag="ACG827_001650"
     CDS             15021..15818
                     /locus_tag="ACG827_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0483794.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15832..16665
                     /locus_tag="ACG827_001651"
     CDS             15832..16665
                     /locus_tag="ACG827_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0483795.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16683..17540
                     /locus_tag="ACG827_001652"
     CDS             16683..17540
                     /locus_tag="ACG827_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0483796.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17562..18071
                     /locus_tag="ACG827_001653"
     CDS             17562..18071
                     /locus_tag="ACG827_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0483797.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18108..18509
                     /locus_tag="ACG827_001654"
     CDS             18108..18509
                     /locus_tag="ACG827_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483798.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18621..18974
                     /locus_tag="ACG827_001655"
     CDS             18621..18974
                     /locus_tag="ACG827_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483799.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19008..19451
                     /locus_tag="ACG827_001656"
     CDS             19008..19451
                     /locus_tag="ACG827_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0483800.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19468..20553
                     /gene="uxuA"
                     /locus_tag="ACG827_001657"
     CDS             19468..20553
                     /gene="uxuA"
                     /locus_tag="ACG827_001657"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0483801.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20546..21259
                     /locus_tag="ACG827_001658"
     CDS             20546..21259
                     /locus_tag="ACG827_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0483802.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21466..21615)
                     /locus_tag="ACG827_001659"
     CDS             complement(21466..21615)
                     /locus_tag="ACG827_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483803.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22011..22211
                     /locus_tag="ACG827_001660"
     CDS             22011..22211
                     /locus_tag="ACG827_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0483804.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7662 a         3418 c         4601 g         6566 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta
     1801 gctcagattt gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta
     1861 gataatagct ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc
     1921 cgttttttag tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat
     1981 caaaattcat ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa
     2041 gcttggagaa attttctcct tcatttgctt ctttttttgc tttatctgct tcttctttag
     2101 tcgaaaatag aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta
     2161 gatctttatc tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt
     2221 taaagagttt cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt
     2281 ttggaccgag ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct
     2341 cttttccgta tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat
     2401 tatttgattt aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa
     2461 acgttgcaat cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg
     2521 aaaggcacat aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa
     2581 cagtctaaca gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt
     2641 cttagttcgt aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga
     2701 caagaaagaa aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta
     2761 ttcggttgat tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca
     2821 ataatgcact tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta
     2881 cggtcggaca tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa
     2941 aaagaataga agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag
     3001 gtctcgttgt tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg
     3061 cgtatcggtt agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat
     3121 ttattgcagt gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc
     3181 ttagatttgg tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg
     3241 gattgaatcc taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt
     3301 tcagcgcact tatctacagt acaggaagtt tatttacggc tattggtgct catattactt
     3361 ggaattggac acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt
     3421 ttttctccac tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg
     3481 aagcatctat atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg
     3541 catataaaac aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag
     3601 caaggctgat atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca
     3661 aattcaagcg aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca
     3721 tcattttcat tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag
     3781 aaatgaagaa aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa
     3841 tgattggaat cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga
     3901 aaactgatcg gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa
     3961 acaaaagcgt gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg
     4021 gaagttcaga aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta
     4081 ttgagtatac aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata
     4141 aagttgattt gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa
     4201 gcagttggac agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta
     4261 cagaaaacac gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa
     4321 acacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcatga
     4381 gtagtgagag tagcacagga agcacgagca gcgaaagcag cacaggaaaa gtaaataatg
     4441 aacttgattt ggtacctatc ataccaacta aggaacaaaa gagaaatgca gggagcaacc
     4501 taaaaagtgt gctaaaacag cctgattttg tttctcgtgg aattgatcat caagaaaaag
     4561 cgaaagaaac agataataga aaaagcgatt taccagaaac tggtagtaga accttgaaca
     4621 agctaattac atggatgggt gtgttactca ttttgattgt aggagcaagc tattttcgtt
     4681 ctctcttcca tcgagtgaaa taaagaataa aagcaacaga tctaaaattg aataaaacga
     4741 tagatttttg agtgaatatt tcattcgaga gtgctgtatt gcctggcgaa gttgtagctt
     4801 tgccagatct atttttgttt actaacatat ttgagaaaca ggaaataaca ggaacacaaa
     4861 tggaaaataa gagcaagaat aattgacaat gcacactgcg catggtagta tctctgaaaa
     4921 atcagtaacg aatctatttg tacgttattc atgtagctgt cggaataagt ctaaataata
     4981 gataaaaagg agtatgtact ttgtttgaag aatttattga tatcaatgaa aggcaagtat
     5041 atcaattttt gaactattgt tatgaaagag acgagaaatt atatgtggtg aaggatatcg
     5101 ctttagatct aaactacacc ctggctaaga tgaattcggt gattcaacaa gcagaatcgt
     5161 tttgcgaaag gtatccagaa tataaattat cttttctttc agaaaacaag atgataaaag
     5221 tcgagttttc ttctcagttt ttactgagta aagtttattc tatactttta gagggaacga
     5281 ttggttatat tttactggat tcactttaca aaggtactta tcaatcctta gaaaatctta
     5341 gtcaaaaaat tatataagtt tgcgtacgat tcaaagaaaa ttaagagaac ttaatactat
     5401 tttgaaaaac tatcatttat cttgcagctt gaaaaaaggt aatccgatag aaggagaaga
     5461 ataccgtatc cgatactttt ttcacttgat gtattggcag tcattcgacg aaaatcattc
     5521 tgatatatta tcaagtataa aaaataaacg tcaactatta gaaaaatttg cgaaacatgc
     5581 gccatatacg cgtagtatag atagaaagaa atggatcagc ctactaagaa tttcgttgca
     5641 acgaatgaaa caaggccact cgattacgta tgtcccagaa gagatgaaca aatttgtgca
     5701 tccattgata acaaaagaag agtttacgca ttacatacta gagccttttt ttgaagcaaa
     5761 cttctggtcg atgaatcaca tcactccaac agagcaagct tacctttatt ttatgtttgg
     5821 tgtaatgaac acctatttgg aagaagattt gttggatact ccagataaag tacaagaaag
     5881 ttggggctgg attgaatcgt ctattgtaga aaattttgaa aaaaacttca agatttgttt
     5941 tacaacttca gaaaaaaatt atctatatgt caatttggca atgatccatt tgtattcaag
     6001 ggtttttggg acaaaaaaga aggtggatgc ttttggaaaa tcagcagaag acagagattt
     6061 tcagcatata tttccagtga tgtatccaat catgcaacag ttttttagtg gggtagctcg
     6121 cagaattcca gagcttaata cttattatga aaagaacaga cggttgattt ttcaatactg
     6181 tatgttagtc agggatattt ttataaaaca cgaacaacct gtcatattct atattcaatc
     6241 aaagtatggt aaaacacagg aattatgggc aaagaaaagg atattatcta tgactgaacg
     6301 cccaataaaa tatagtgcat ccgctgatca gttacctgat attgtcatta gcgattatcc
     6361 tatcgacaaa aaggcatatg atcaaacgaa tacacagata ttttactgga atggacaacc
     6421 tacaaaaaat gattgggcgc gtctgaaagc tgaaatcata aaaatcagga atgagaaaaa
     6481 tagatccata ttggaaaaat tggatttcgt taattaagaa aagcagattg tttatgccaa
     6541 aattaagaca ttgcaaaaag attattttct ttaaaatcga caagatgacc agaaagcaag
     6601 agttcgttga gctcttgctt tcttttagtt ttagaaagga acatcctgat tttatcttcg
     6661 ctaaatccag tcaaaagttt tgcagattgg tcgtagataa tcggtttttt caataaagaa
     6721 ggggatgtac gaatctcaat atatagttca ctcaaactga gttcatctat tctgtgaaca
     6781 agatttttat aactaataga atgagtagag agcatttcgg tcacgccgtt gtttgtcaat
     6841 tttagtaagt ggataaattc atttttggtt agtggttgac gaattaagtt ctgttcgata
     6901 aacggtatat catggacgat aagccaacgt tttgcgcgtc ttgaagacga gcagccagaa
     6961 cctgtataca atttaatcat catacattcc tccaatagct aagtatttat gaaataataa
     7021 catgaagaaa tagtcgaaaa gtgccattta gaggtgtaaa ataagaaaat gttgtcacgt
     7081 ataatggaac tagctagata agaaatacta ttaaatcaag aaaaaagaaa gaaggcacaa
     7141 gtatgtcaaa cacagccgac gcaatagtag tgatcgattt acaaaaaggg gtatgttttg
     7201 attcttcgcc gatacacgaa ttagaaacag tgacagcatt agtgaataaa agaatcaaaa
     7261 catacgaaac gattgggaaa ccagttattt tcgttcaaca cgaagatgct caattagtgc
     7321 cacaaacaaa accatgggag ctattgtcgg aattatctat tcctgagcac agttattttg
     7381 tgagaaaaac ccatgcgaat tcattttata agactaattt gagtgtttta ttaactgaat
     7441 tggactgtca ttcgatcgaa ttatgtgggg cacaagcaga gtattgtata gatacgactg
     7501 tgaagtttgc tcatggatta ggataccagc ttatgatgtg tagaggagca acgacaactt
     7561 atgataatcc ttttatgaaa gcaaaggaaa caatcgcttt ttacgagaaa atttgggata
     7621 aacgatttct gacatttttg gagcaataag ttgtttcttc gtttaaaaaa agcagaatgg
     7681 atactggttt atccattctg ctttattacg taaaatgaga aaataatcct gttctatttt
     7741 tatgggataa accatttatt ttttgtataa aaaatgtttg cgtttacata taattctcct
     7801 agaaacataa aaaggagtga atagcttgag aacaaaacaa gtattaaagg gagcgttggt
     7861 catcttattt gctgtaagta caagcgtccc gcaatggaca gtatatggag agagcatttc
     7921 ttccgaatcg gaatctcggg aggtagaaac cacagaaact actgatgctg gaaatgaaac
     7981 gaaatcgagt acttcacaaa cagaaacaaa tactgaaaca acggttgaga catctgttac
     8041 tagcacagat caagaaacga ccgctaccac agataaccaa caaacagaag aagcaacagc
     8101 tacaaaaatg tcaaacgacg aatcctcttc tgctaaagta agtgacaaag agaatgggac
     8161 agaaaatgat gatatcggta aaggtgaaaa tttcaatggt aaagaatatt tgaattactt
     8221 cggtaaacag atgacagaaa tacttaatgg accaacagaa gacatcaagt caaaattaat
     8281 agagttatta gtcactgagc ttgctgttaa tgattatggt gtagataaag agattatcaa
     8341 acatctgatt gctacttatc tattgtacga tatcgctttt gcaaaaaaca gtgtgttgat
     8401 gtcgatgctt ggaggtacag atgatgggac aacgcctaca gaaaacacag gcttctatcc
     8461 aaatacaaaa caacttcatc aaactgtact aaacaaacaa gatggcaaaa tgatggatgt
     8521 atatgcatat tatgtcgatc aaggttcaga taaaacagtc atgatccatg gcggatttcg
     8581 agggaattgg aataacggga tcgttacaga agaatacaat gatttttata aggccgggta
     8641 taatttatta tttgttgatt ccagagcgac tggtaatagc ggaggcgatt atgtgaccta
     8701 tggacaatat gagtcagatg acgtccttta ctggataaat caagaagttc gagagcggcc
     8761 atctcaaaag attttacttt atggtggttc gatgggagca gctacgatga tgtctgtatt
     8821 agcgaaagat attcctgtca atgtcaaagg aattattgaa aattgcggat ttgcatcaat
     8881 tgatgaacaa ctacgtttta cgtacagcca gacagtagcc cccgcactac cggatgccat
     8941 aaaaaatcag ttagacatca ttggggatca agagcatgaa gatttattta tgggcctttt
     9001 gaaacaatac tattttgatc aggagatgca tttggataca aaagctgctc tacctacgat
     9061 tggaatgtca gattcattgc ctaaattgat tatccatgga acagcagatg atgtagtacc
     9121 tgtgtctaac gcgcaaaaat tatatgagtt atcaggaggg tataaagatc ttttattagt
     9181 agaaggagca gggcatggaa aggcacagga agttgaccat gctgcctaca cgaaacatgt
     9241 aacggacttt ttgaaagtag tgttccatga tcagataaac gtaaagtatg tggatgaaaa
     9301 caataaaagt ctactcaaag accaggacga aatccagctt tacggagctt atggagaaaa
     9361 ttatatgaca gagcaaaaga cattcgaagg atacgaatta gcaaatgttg aaggaccgac
     9421 tgaagggatt tttaatgaga cgataccaac tattattttc tattacaaaa aaatcccagt
     9481 agctccacca aaaaagcaag atccgacaga gaatgcagac aataagggaa agaatgaacc
     9541 tcgagaggat caatcaagtg atgatacgca accgattcag atgacagtga aagtacaaaa
     9601 aaagacagat gcaaaaaaaa gaatgttgcc gaaaactgga gaaaagaaac attcgcttct
     9661 catttattca ggtggatttt tatctgttag cacgattatc acatggattt ggcgaagaaa
     9721 gagagggtaa atgaatgaaa tggttcaagc gaatagtgat tgcagtacca gttatcatcg
     9781 tagttatttt agtcactgta tttttgatta atcaaatcac accaaagccg attagcttgc
     9841 ttgttagaaa acaatttgac acaagtgaaa aagaacaagt atatgcccga ccagatgatt
     9901 tccagcaaaa aatcgaacaa atcgatataa aaaaagaaat atcctatcct tcaaagtaca
     9961 aaaataataa tatggatatc tacacaccga aagtggaaaa gaaaaaatta ccgattcttt
    10021 tctggatgca tggaggcgca tatgtaggag gggataaaaa tgattgtcgt gattatttgg
    10081 aatatctatg ttcagacacg cagcaaatca tcgtaaatat agattatgaa cgatcaccgg
    10141 aagcaaaaca tcctacacca gttatccaat tgaacgaagc cattcaagca gctaaaaaag
    10201 agataaaaga tcaagcagat tggtcgaata tttcgattgg tggaaattcg gcaggtgcac
    10261 aaattagtgg agaatatttg ttagcacttc aaaatgaaga aattaaaaaa gctgatcatc
    10321 ttgatccgac tttggagaac aagcagatta cgaagtttat ttctttgtct ggtctgttgg
    10381 atccttctgc acttactcaa gtaagtgata aaatctctag ctttctttat gaaaaatgtg
    10441 gatgggctta ctttgataat aaggactttg aacaatcaga gaaggtcaaa agtttagcct
    10501 tggtcagaca tgcggatcgt tggacgcaaa acacattctt aacggatgga aatacaaata
    10561 cgtttacgaa acaaatggaa gaagtaactt ctgtatttga gaaagcgggg gtgaaagtca
    10621 caaaagtaga ttatgcggag aaaaatgctg ttttgaacca tgaatatcag tttgatttca
    10681 gcaataaaca agcgcaagaa acatatcaaa agttagtaat gtttttcaag gagtaaagtc
    10741 ttatgatgca aaagaggttg taatctgtca cttagctcca agtcacaagg agcaatttca
    10801 ataaacaagc ttttcatggt ttatgaatat tgtaacttgt gactgaggag ttagagatgg
    10861 caaggggcac cttttattcg aaatttggga atatcacacg attattgtca cgatctcttt
    10921 tacgtaaatt ttataagtca gatttgttgt tttgatcgtt taaaaaaagc aacaaatccg
    10981 aagcctgcgg aggcaagaag ggacaaaaag ccgagtggta agcaaaagaa tgctggttcc
    11041 actaatattc cgttgttatt gatataaggc tgggaacaaa gatgaatagc aaataaaatc
    11101 aatgcactag cgaaaaaaag caaagaaata tggatagctt tcataagcag cacctccttt
    11161 tttataattg tatgataaat agattaagaa tctataaatg tgacaagttt gttaggatgt
    11221 gaataaaatg atcatctagc gaacttgaaa caaagatggc taatgataag caattaatga
    11281 ttcagcaatc agataggtac aaagtttacc atcatacgtc tagaccaata atagaaaata
    11341 tgggaatata atagaaaaga tttaggatac ccccaaaaga atttccccaa aaattcgatg
    11401 tatcctaaac ttttttattt tacataacaa tcatatcaac tgtgaaataa aattacaaat
    11461 gataacctaa aatgaggcca aaagccagtg ttatcaagga aatacttaat tcaccaaaat
    11521 tttttttaat tttttagaaa ataagaaaca aaaataataa aaaaaagaaa agataaattt
    11581 tttactactg tatactaact ttgttatcgc atctaacaag taactcgaat aataccaata
    11641 caaccttctc caaagatgcg atgacatacg ttctaaccca ccgactattg atgttctgta
    11701 ccacccttgc agaaccaaaa aagccactac caaatatttg gtagtggctt tttttgtttt
    11761 atggattttt cgctttttct gttatggtgc tattctttga tttcagaaat agtatgtcga
    11821 actttagcga tagaacgacg tgattctttt tcgttgtaaa acatcaaaat cacatataga
    11881 gagtccaaaa tgcttagttg agaaatacgg gaagctaaag cttcagatcg gaattctgtt
    11941 tcttcagaaa tagaaataaa aacgacatcg ccaagtttag caagggaaga attagcttgg
    12001 ctagtaataa cgattgtttt tgctccagtt tttcttacgg tttcagcaat atggattgtt
    12061 tcttttgatt gccctgtgtg agaaatcaaa acagcacagt ctttttccgt caataaagaa
    12121 gcttccatca attgaatatg gtaatccgtg ctgtgagaag ttgcgatagg ggaacgcaaa
    12181 aatttatgat aagcgtcagt tgctacaatc tctgaaccgc caacaccgaa aaaatagaca
    12241 cgtttagatt cattgataat agtagccgct ttttttagat ccttttgttg taacaacttt
    12301 tttgtgtcat ttaaagttga aatactagac tcgaacacct tttgcgccat agtgagctca
    12361 gtatctgttt tctgaatatt ttcgtggata gaaatagcag atagatcatt ttcttggatc
    12421 aacatggcca ttttaaaatc tttaaatcca ttataaccaa gtttttttgt aaattgaaaa
    12481 aaagtagagt cagcaatgcc cagttcactt gctaaattgc taataggact atgacttact
    12541 ttgcttggat tttctaaaat ataatcggcg atagcctgtt cttttgggct gaagtcgttg
    12601 tacattgttt tgatttgtag tcttatggag acgctcatga gaattcctca ttttcataat
    12661 aagattatca ttttttccgt tctcattaat gttaaagata caaggagagt aggaaaaaat
    12721 caaacaaaaa atatttttgt aaaatctatt tacaaaaaaa tttttttgta taatgagggt
    12781 ataagaaatg gaggcgctat catggaaatt ggtgtaattg gtttgggaaa aatgggattg
    12841 aatttagctt taaacttaaa agagcaccat cacaaagtac aaggactcga catttcaagt
    12901 acagctgtta gtcaggcaag agaaaatgac attcctgctt atcaagagat ctctgaattt
    12961 ctagctactt ttgagcagaa aaaaatcatc tggttaatgc ttcctgctgg agagataacc
    13021 gaaaacatgt taagaaaact ttttccaaaa ctcggaccag gagacattat tattgaaggc
    13081 ggaaactctt attataaaga ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc
    13141 ggttacttgg attgtgggac ttctggagga atcgaaggag cacgacatca agcctgctta
    13201 atgattggag gagataaaga aacttttcta tccgtggaac aattattcaa agatgtagca
    13261 attgaaagag gatatctgta tgctggtgca tccggaagcg gacacttctt gaaaatgatc
    13321 cataatggaa tcgaatatgg aatgatgcaa gcaatcggtg aaggattcca acttgtacaa
    13381 aaaagtgagt atgcttttga cttggaacaa gtcgcacgtg tttggaatca tggttcggtg
    13441 attcgcggct ggctgatgga aatcatggaa gatcagttaa acgaacatcc caatttaagt
    13501 aattatcggg gaatcgtttc tgcatcaggt gaagcaaaat ggacggttga aacagcgcta
    13561 gaaatggctg ttccagttcc agtaattgca actagtttgt tcatgaggaa tctttcacag
    13621 gaagaagata gtttttcagc aaaagtagtt tcagcactaa gaaatgggtt tggcggacat
    13681 gaaatagttg aaaagggtga gtaaaatgcc agattatcat atagcaatcg ttaattccag
    13741 cagtttcgga agagtctttc cacaccactt ggaaagatta aagtcagttg gagacgttca
    13801 tttctttact gttgatcaag agattccagg aaaagaacta gcggagttac ttaaaggatt
    13861 caatatcatt attgcaagtg ttacgccatt ttttcgacga gatttttttg aaaacaaaga
    13921 tgaactgcta ttgatttccc gacatggtat tggttacaac aatattgatt taaaagcagc
    13981 aaaagaacac cagacaatcg tatctatcat acctgcttta gttgaacgaa atgctgtagc
    14041 agaaaataat ataactaatt tgttgtccat gcttcgacgc acgacacaag cacaagcaag
    14101 agtcaaggac gatcgatggg aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa
    14161 aacagttggt gtgattggtg caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa
    14221 cggttttcgc tgtgatgttc tagcttatga tccttacaaa tcagatttat acatcgaaag
    14281 ctttggtgca aaaaaagtag aacttcaaga attactggaa caatcagata tcatttgttt
    14341 atgtgcgaat ttgacagaag agaactatca catgatttct gataaagaag tcgatcagat
    14401 gaaagaaaat gtttatctat ctaatagtgc aagaggcgct ctagttgatg aagaagcgat
    14461 gattaatggt ctgaaaagcg gaaagatcgc aggatatgcc acagatgttt tagaagaaga
    14521 accgggacgt caagatcatc cgtatttagc gtttgaacaa gtagtgatta cgccacatat
    14581 atcggcctac acaagggaat gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga
    14641 acagatcagt cagcaccttc ttcctgaacg ttctgtccag acaaagagca actatataaa
    14701 aggatgatga agaaaatggt gaacggttta aaagtgaaaa caggccctca attttattta
    14761 tatgaagaag gtggtatttc aaaagtttct gatctcttga aatcatacgg cgcgaaaaga
    14821 gtactggtca cccacggaac ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg
    14881 aatgacgaaa cgattcaatt tttttatcat cgttattctg gagaatgtag ttatgctgaa
    14941 gccagacgaa tagctacaat catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg
    15001 gaggaaaact gaccgatcta gtgggttata gtgccaatct tgcaagaatc gatttcggag
    15061 tcatccctac tttagctagt aattgtgctc catggacacc tcttgctgtc atgtataagg
    15121 agaatggaca agcagaaggg aaaacagaac acttttacag acaagctgct ttttttataa
    15181 cagatcccca attagtgatt gattcccctg ttccatattt cattgccggg ttagcagata
    15241 cactagcaaa atggtatgaa tctgagctca tcttagaaca agattttttg caagatgaat
    15301 catttttgaa gctagcacaa gatactgcga agatctgcaa agaagagatt ttgaatcatt
    15361 ctgctaaagc aatccaagat atggcacaaa gaaaactgac atcggagttc aaacagcttt
    15421 cagagattat ctttgctgtt gctggtttag ttggaggatt aggagataaa tacgcaagaa
    15481 atgtggcagc acatgctatg catgacgcta tcagtaaata tattccggaa agccatcgtt
    15541 ttctacatgg agaaaaagta gcgtatggga ttttctatca actggcacta gaaggtcgct
    15601 gggaaaaaat agacgagtta tatcctttgt atcgggaatt agaactgccg gtttctctag
    15661 aacaaatggg attatttccg aaagataaac agataataga taaaatggct gaatttattg
    15721 attcgaaaga gaaagtgcat ttgattccta tagaggtaac acagaaatca ctgatacagt
    15781 caatgatgga actggaaggt tatatcagaa agatctgaag gaggaggaca catggaaaca
    15841 ttaagtgtag tacaaagttt gttgattgct ttatgggtag ccgcggttat gtcccgctgg
    15901 ttaggtggag gagccacgtt gaccttacgt ttttctcctt tgatgactgg attagtcgtc
    15961 gggttgatca tgggggaagt tgcacaagcg atgattgtta cagccgcttt gcaaatgatt
    16021 tatatggggg ttttttctcc aggaggatca atgccagctg aaccatctat tgcagcagct
    16081 atcgcagtac cagtcgcatt attaggggat ctgtcgccag aagcagcgat tgccgttgcg
    16141 gtgccagtcg gattgctcgg aagttacttg tatcagtttc gttttttcat caatactttt
    16201 ttaggtaaat atacagacaa agctgtagaa gatttgaata caaaagctat ggcccgatca
    16261 attattcttt atccaacggt tgcctcattt ttattattcg ttccgcttgt tttcattgct
    16321 ttatatttag gggcgccatt gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt
    16381 atccacattc ttgaagtagt aggtggaggg ttagcggcaa tcggtatcgc gacaactgta
    16441 tacgttatcg gtcgaaaaga ttatctggtc tttttcttcc ttgcttattt tatgagtgtt
    16501 gttttgaaat ctttagatat tacgatggtt acttatgcca tttttgggat tattatcgct
    16561 ttgatctttg tccagtcgca aaaagggaaa caagtacagc ctgccacaag caataacaat
    16621 tcagcatctt ttgatgacga agatgactat gatgacggat tttaatttag gaggaatggg
    16681 aaatggctga aataatggat aatcaaacag gtgctcctga agaaatcacg aaaaaagatg
    16741 taacaaaagc ctatctgaga tggcattttg caaacgaaat tcctcactct tttgaacgtt
    16801 acttggcgcc atcactacta tacggaatga tgccaatctt gaaaaaactt tataaagatg
    16861 aagactcttt aaaagcagca tataaacggc agttactgtt cttcaacact caattaagct
    16921 ggggtggcgg tgtgattact ggcctaatgt cttctatgga acagcaaaga gcagaagaag
    16981 agtatgaaga aaaagagatc ttgatgcagg atgacttgat gtataacacg aaagcgggat
    17041 tgatgggggc actagcagga atcggagatg caatcgattc aggaaccgtc caatatattt
    17101 ttatcgcaat cgcggtacct tgggcacaac agggaagtcc tttaggcgca ctttttcctt
    17161 ttatctgctt tgggttgtat caggttttac taggggtatt tttcgcaaga caagctttca
    17221 ctatgggccg taatgctaca ggcctaaagc aaaacactgg tgttcagaca gcgattgaaa
    17281 tgctatcagt tttagggttg tttatgatgg gggtgcttgc gggaaactat gtaaaagtag
    17341 aatcttcatt gaaatttgcg atatccggac gtgagtttgt tatccaggaa attcttgatc
    17401 aaatcgtgcc agggatatta cctttagctg tcgtaatggg tgtgtacttg ttctatacga
    17461 aaaaaggatt gaaagtcaca caagcattat tatggctgac tggaatatta attgtgctag
    17521 caggaatcgg tattttataa aaagaaggga acgtgaataa gatgggtgta gtcaatttag
    17581 cgcgtgtgga tgagcgtttg atccatgggc aagtaatggt aacgttatct caaaaaagtg
    17641 gagtgaattc catttttgtt gtagacgaag ttgtagcaaa agataaattt atgagagatc
    17701 tttacaaaaa tgcaggaagc cgtacaggac aaaaaacaat tgtcattaca ccagagaaag
    17761 ctaaattcta ttgggatgaa tatcaattta aagaatacaa ttgtattttg atcgcaaaaa
    17821 cagtccaaac gatttacgac ttagtaaaac acggaatccc catggaagaa ttgaatattg
    17881 gagggattgc tcaaaaaaat cctgatacag atcttttagt gacgaaatct gtttatttaa
    17941 ataaatcaga tgctgaaaaa ttgaaagaaa tgaatgaaca atatggcgta aagaatattt
    18001 attttcaagc cactccatct gcacctaaaa caggattaaa agatgtattg aacaagtttg
    18061 atttgtcata aacaaatgct tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg
    18121 agctgtttcg tgattgggaa tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa
    18181 agagtaaaca gcgttttctt tcagcattag tatcagatat ttacagtatg agaacagatg
    18241 tgacagtcat tgcttacgat acgctagcct accgatcaaa aaatatttat gttggagata
    18301 tcgaaaaagc agagaaagtt atctgtactt attacgatac acccgttcat gcattaggaa
    18361 gttatttcat gtttgattgg aaagaccaaa ggaaaaaaac aatctactct atattattgt
    18421 ccttcatttt gttgttcagt ttaggttggt ggggaatgat gatatataac aaaaatcctc
    18481 atcacgtatt cgatctgcta tccgtataaa caggtatcac tgtactagca tttgggagct
    18541 actttttctt attgggaaaa gcagctcgcg gatggcctag cagacaaact tttattcgaa
    18601 atacttcatc catattaacg atgctagaaa tgatccgtac gattgatgat ccgagtgtag
    18661 cgtatgcgtt tgttgatgaa ggctgttatg gagaaaaggg cttagacagc gtgagatcgg
    18721 gtatgaaaaa agaggctatt cttttttatc tagactctgt tggggcagat acaccgctac
    18781 agttttcggg aaattatttt tcaaataaag aacagtggct gaaacaagtt gataagttga
    18841 aagaaaaaaa cgtcaattat atttttagtg caagaaaaaa gcaagcgcag ttcttctatt
    18901 tgaccaaaac agatttacga gggaaaacat ttaattggca aaatgccaat cagattattg
    18961 ctctttttcg ataagtaaaa agtgcaaaac aaggagtgag aagactaatg ttggaaatca
    19021 tgatcgttac acatggtaag ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg
    19081 ggacatcgag ccatattgaa acaatgtgct tagagtctgg ggaggacatc caaacattcg
    19141 gagagaaggt caaagaaaaa ttaactatga tcaacacagc agaacccatg ctagtatttg
    19201 ttgatctaat tagcgcaagt ccgtacaatc aagcgctctt ggcagtcaat gaactgactg
    19261 aagaaaagaa agcagccact tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag
    19321 caatcaatca tcagatgctt cacacaccga tagaagaagc ggtggaacaa attgtcaaac
    19381 aaggaaaaga taatttaact gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg
    19441 acgattttta gaaggaaggc tgaaaagatg aagtggggat ttagatggta tggtgaaaaa
    19501 ggggattcga tcccattaaa atatgttcgc caaataccgg gaataaatgg gataattgga
    19561 actttgctga ctaaaatgcc tggagatgtt tgggagattt cagaaattga ggagttgaaa
    19621 gcatctgttg agagccaagg actggaactg cttggaattg aaagtgtagc gatccacgat
    19681 gcaatcaagg cagggacaga tgaacgagat tactatattg ataactatat acgaacaatc
    19741 aaaaacttgt ctcgatgtgg ggttcgattg atttgttata gcttcaaacc aatttttgga
    19801 tgggcgaaaa ctgatttgtt taatgaaaac aacgatggaa gctattctct tttatacgat
    19861 cagaaaatcg tcgatcagat ggagccagga gaaatgtatc aattgataca cagccagtca
    19921 aagggattca aattgccggg ttgggaagaa gaacggttga aaaaattcgc tcagctggaa
    19981 aaactgtatg aaggaatgac cgaagaagaa ttatttgaaa atctgactta ttttttagaa
    20041 aaagtgattc cagtgtgcga aaaagcagat gtaaaaatgg ctatccatcc agatgatccg
    20101 ccatgggaac tttttggttt tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt
    20161 ctaaatgcag tagattctcc atataatgga ctaacgctgt gcacaggatc tttaggggca
    20221 aatccggaaa acgatttagt tgaaatcatc catgaggtag gttcgcgcat ccattttgtc
    20281 cacttcagaa acgtggagtt tttaggagaa agaaagttca aagaaacggc acatccctct
    20341 acagaaggct cccttgatat gtatcatatt atgaaagcgt tggtgcaaga agggttcgac
    20401 ggtcctatac gccctgatca tggtagaacc gtgtggggtg aagtggctat gcctggatat
    20461 ggcctgtatg atcgtgcaat gggactcact tacatgcaag gtctatatga ggcgataagt
    20521 aaggaaaaac cactgggaga gcattatgaa taaaatcaag ctcttcatct ttgatatgga
    20581 tggcctttta ttagaaactg gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa
    20641 gtatgattat gaaatgcgta aggaagtcta ttatttacta actggccaaa cagaaatggc
    20701 gataagagaa caaatgggca ttttgtacgg agaagatgtt ccatatatca aatggcgaga
    20761 agcaatcaat caatataaag aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa
    20821 aggagcagaa gaaattttat cttttgcaaa agaaagagga atccatacaa tcgtggcttc
    20881 gtctaataca cgggtaaaag tagaaatgta tttaaaaatg gaaaatctat atgccttgtt
    20941 tgatcaaatc atttcagggg atgatgtaaa aaaaggaaag ccggaaccag agatcttttt
    21001 gaaagcttgt tcaaaaatga atattcctcc aagtgaagca cttgtatttg aagactcgat
    21061 tgcagggatc gaagcagcaa ggcgagcggg cattttgtct tttctcatca aagatcattt
    21121 gatagggttg cccgatcata atgggaagca taagttgaaa gtagatgtaa gtttatatga
    21181 atccaataaa gcagactatg aattttatga cctgcatcaa gcaaaacgat ttttagaaag
    21241 taaagggttg tatctctaat aaagaggact attttctagt cctctttatt tattgtcctg
    21301 cacgactgag ggaacagatg aaaaagaagt aattttttat tttgaagtat ctaccttatt
    21361 tgtagtgaac aatttagaac aaacacaaaa cgcatctact gttatctacc agtagatgcg
    21421 tttaaagaga agaacgagaa tcgtccatat gagggtttcc actccttatg cagatttttt
    21481 taattcttgc actgcttgtt cgatcgtaac acctgtcgct acatgattgg tattttcttt
    21541 gtcaaaaacg acaaatagat tcaaatctgt atccaaggtt actcgatatt gtatatcttt
    21601 gttatagaat gtcatagcca ttctcctttc tttaaatact tgctgaaagg caacgaacgc
    21661 gttgataacg aaattccatt tataagtata ccaccaattg aaaggttttc atagttaaga
    21721 aattttaaag tcaaaagttg cagataaaag caaataaaca agaaaacaaa aagtgaatca
    21781 atcgcggtta ttcaactttt tcttcgattc gaagacaata gaagacaatg aaatttatat
    21841 ttcattgcat tatccttgtg ttattagaaa aagcatgata tattaaatga gtaatctatt
    21901 ttgaaacgta atttgagcga tatatctaca tagataaaac tccttttgcc tatcaaatat
    21961 ttattgcaac gaaaaaacgt acattgatac gtatcaggag gaatttattt atgaataacg
    22021 gtacagtaaa atggttcaac gcagacaaag gttttggctt tatcactggt gaagatggaa
    22081 atgatgtatt tgcacatttt tcagctatcc aaggtgacgg cttcaaaact ttagaagaag
    22141 gccaagcagt gtcttacgat gttgaagatg gtcaacgtgg ccctcaagca acaaacattg
    22201 ttaaagctta ataaaaattg acttttaacc gtcttttcga aagaaaa
//
LOCUS       DAZKOU010000046        22208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000046 DAZKOU010000000
VERSION     DAZKOU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.046"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..2113
                     /gene="tdc"
                     /locus_tag="ACG827_001661"
     CDS             278..2113
                     /gene="tdc"
                     /locus_tag="ACG827_001661"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0483805.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2418..3896
                     /locus_tag="ACG827_001662"
     CDS             2418..3896
                     /locus_tag="ACG827_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0483806.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3862..5193
                     /locus_tag="ACG827_001663"
     CDS             3862..5193
                     /locus_tag="ACG827_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0483807.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5307..7859
                     /locus_tag="ACG827_001664"
     CDS             5307..7859
                     /locus_tag="ACG827_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0483808.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7866..9305)
                     /locus_tag="ACG827_001665"
     CDS             complement(7866..9305)
                     /locus_tag="ACG827_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0483809.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9280..9462
                     /locus_tag="ACG827_001666"
     CDS             9280..9462
                     /locus_tag="ACG827_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483810.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9651..10013
                     /locus_tag="ACG827_001667"
     CDS             9651..10013
                     /locus_tag="ACG827_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0483811.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10200..11057)
                     /locus_tag="ACG827_001668"
     CDS             complement(10200..11057)
                     /locus_tag="ACG827_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0483812.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11177..12088
                     /locus_tag="ACG827_001669"
     CDS             11177..12088
                     /locus_tag="ACG827_001669"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0483813.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12095..13741
                     /gene="mdcA"
                     /locus_tag="ACG827_001670"
     CDS             12095..13741
                     /gene="mdcA"
                     /locus_tag="ACG827_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0483814.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13843..14142
                     /locus_tag="ACG827_001671"
     CDS             13843..14142
                     /locus_tag="ACG827_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0483815.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14132..15808
                     /gene="mdcD"
                     /locus_tag="ACG827_001672"
     CDS             14132..15808
                     /gene="mdcD"
                     /locus_tag="ACG827_001672"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0483816.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15812..16405
                     /locus_tag="ACG827_001673"
     CDS             15812..16405
                     /locus_tag="ACG827_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0483817.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16429..17505
                     /locus_tag="ACG827_001674"
     CDS             16429..17505
                     /locus_tag="ACG827_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0483818.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17530..18399
                     /gene="mdcB"
                     /locus_tag="ACG827_001675"
     CDS             17530..18399
                     /gene="mdcB"
                     /locus_tag="ACG827_001675"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0483819.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18510..19274
                     /locus_tag="ACG827_001676"
     CDS             18510..19274
                     /locus_tag="ACG827_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0483820.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19763..21856
                     /locus_tag="ACG827_001677"
     CDS             19763..21856
                     /locus_tag="ACG827_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0483821.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21881..22183
                     /locus_tag="ACG827_001678"
     CDS             21881..22183
                     /locus_tag="ACG827_001678"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0483822.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7192 a         3789 c         4731 g         6496 t
ORIGIN      
        1 aaaaatatgt ttagtgaatg aaggacagtc attttatttg tttgttcgct ttttttaggt
       61 tatctatttt agaagactga tttattgaaa aattgttcgc ttttttgcgt tgaattatca
      121 gaaaaatccg actttattta tttttgtttg taaaaatcta cgtggatttg atgttataaa
      181 aaaaaaattc tcatttttgg cgctatagtg aagaggtgtt acagataaac acatgtatct
      241 gcaagacatt gaccaacaaa atatggaggg aagaaaaatg aaagatatgg atatcaaggc
      301 cgtctttata ggcgacaaag ctgaaaatgg accagtttat aaaatgttgt tgaacaagat
      361 ggtagatgaa catcttggat ggagagaaaa ttatattcct tcagatatgc ctgcaattag
      421 cgaaggagat aagctcactc cagattatct agccacccgc gaccatatga tagaggtctt
      481 ggatgaagtc agtcagcgat tacgcgcagg atcgatccca tggcattcag caggccgtta
      541 ctggggacaa atgaatgctg aaacattgat gcctgcttta ttagcttaca actatgcgat
      601 gctgtggaac ccaaataatg ttgcattgga atcttctatg gcaacttcgc aaatggaagc
      661 agaagtagga caggattttg cttcattatt caatatgaca gacggatggg gacacattgc
      721 agcagatggt tctattgcta atcttgaagg attatggtac gcccgttgca tcaaatcgat
      781 tcctttagct gtcaaggaag tgctgcctga aaaagtgaaa aaaatgtctg agtgggaact
      841 tttgaattta tctgttgaag aaattttgga aatgaccgaa agctttacgg acgaagaaat
      901 ggatgaggtc aaagcagctt cttcccgtag cggaaaaaat atccaaagat tagggaaatg
      961 gctggtccct caaacaaaac actattcttg gatgaaggcg ttagatattt gcggagtcgg
     1021 cttagatcag atggttgcta tcccagttca agaggattat cgaatggata ttaatgcttt
     1081 ggaaaaaaca attcgtgaat tagctggaca aaaaatccca attctaggtg tggttgctgt
     1141 cgtaggaacg acagaagaag gtcaagtaga cagtgtagat aaaatcgtcc aattaagaga
     1201 aaggttaaaa gatgaaggga tctatttcta tttacatgtt gatgccgcat atggcggcta
     1261 tgcgcgttca ctgtttctaa acgaagcagg agaatttgtg ccgtatgctt ccttagctga
     1321 attctttgaa gaacatcatg ttttccacca ctgcgtaacg attgacaaag aggtatatga
     1381 agggttcaga gctatttcag aagcggattc tgtcacgata gatcctcata agatgggcta
     1441 tgtcccttat gctgctggag gaatcgtaat caaacataaa aatatgagaa atatcatttc
     1501 ttatttcgcg ccatatgtgt ttgaaaaatc agtaaaggct ccagatatgt taggagctta
     1561 tattttggaa ggatcaaaag ctggagcaac cgccgccgca gtctggacag cacatcgtgt
     1621 attgccattg aatgtgaccg gatatggtca attgattggg gcttcaatcg aggcagctca
     1681 aagattcaga gaatttctcg atcatctgac ttttactgtt aaaggaaaaa caatcgaagt
     1741 ctatccattg aatcatcctg attttaatat ggtcaattgg gtattcaaag aacaaggctg
     1801 tacagatttg aacgctatca atgaattaaa tgaaaaaatg ttcgatcggt cttcttatat
     1861 ggatggcgat gtttatggtg aacggtttat tacttctcat actacattta cacaagaaga
     1921 ttacggtgat tctccgattc gttttgttga aagaatgggt ctaacgaaag aagaatggaa
     1981 aaaagaacag aaaatcactc tgctacgtgc ggctatcatg actccttatt tgaatgatga
     2041 tcgaattttc aatttttata caaaaaaaat cgccaaagca atggaaaaga aactaaacga
     2101 aatcatccaa tagaactgat ttttgtgtga aagaataaca agagacatat ggagaacaga
     2161 caattatttg cttcatcaaa acaattgtat ttccccctgt ttttcatata ttcctcgcca
     2221 agatattctt ttttataaaa cgttctataa gttggatcgt tcgtttcatg tatcaaaccc
     2281 agttcactaa gatcgttcaa tggatgtttc ggtgatactg ggttttgtac tgaatcgatg
     2341 aaaccttgtc atttctgttc ttgaacaaaa ataaaagaaa acaaaaacat atgtcgctat
     2401 tgaaagaagg aataaagatg aaagatactg gttcatcaaa gcaaatcacg accatgacct
     2461 ttatcggaat gacctgcgcc cttgtagcaa gtgtacgaaa tattccagat gtagcagcaa
     2521 cgagctggac gatgattttt tacatgctgg ttgcagtatt attgtacgct tttccaatct
     2581 cattgatctc tggtgaattt gctggaatgt ttccccaaaa aggtggtcca gaactttggg
     2641 tatcgaatgc tctaggaaaa aaatgggggt tcgttgtctc atggctgtta tgggtccaaa
     2701 tgtttcctgg gatggtgatg gtcgcttcag cactcgctcc gttgtttggt aatattatcg
     2761 ataatgtgcc gcttggtttg aacagcaaat ttacattagt tgtcatttta gtcgtttatt
     2821 ggatcatcac ttttttgaat ttgaaatttg atatggcaaa aatcggcgga aaagtcggtg
     2881 tttggttagg attgtacgtg cctttgacga tgatgctctt attagggttt gctgcgtgga
     2941 tcaaagttgg gatcgtacca accggaacat taggcagttt ttcatgggac aaattaatac
     3001 cagatactac gactgcatcc tcatttgttt attttgcgcc gatcatgttt atttttactg
     3061 gtatcgaaat gtcctctgtg tatatcactc gattggaaaa tccagttaaa acgtatattc
     3121 gcggagtatt tgccgcgtta atttttatat ttttcgtcaa tattttgaat gctttagttg
     3181 tagcaaatgt cgtaccaaaa ggacaaatgg aattgaataa tatcgcgcaa tctatttcga
     3241 tttattgcca aattcttggt ttgccacatc ttattgtgaa tctgtttagt ttacttgtct
     3301 tcattggtgt agcagtccaa ttatctgctt gggcctcagg accagcaaag acagtgacag
     3361 aaagtgctag aaaaggtgcg tatccaccaa aattcaattt ttggaagacg aatcaatttg
     3421 acgtatccaa atccgttatt ttgactcagt ctgtcattat ctcaatcttt gcactttttt
     3481 atttgctgat tcctggcgtc aatcaagcat ttttgatgct cgtaaattca actactgtta
     3541 tctactgtat cgtctatgtc atcatgggga tcgcagtttt gagattgcgt tatacacatg
     3601 caaagttgaa tcgaccattt agaattggaa aaaaagagaa aaagagcaat cttggagttt
     3661 ggactgtcgt tattgtttta tttgcggcaa tcggattttc tgtcggactg acgatgaaag
     3721 ctggaacatg gatcaatcta attgcagtca cagccatcag tgtcattttg tttgtagtac
     3781 cactctgtat tgaaaaaata aagaaacctt cttgggaaca ggaagtcgcc atgctgcttg
     3841 aaaaagaaaa aggtgaactt gatggaaaaa ccggcaatga aacggactct aactaatcga
     3901 aatatccagt taatcgcatt aggtggcgca attggaacag gattattttt aggttcagca
     3961 aaggcaattg catcagcagg gccagctgtt attctcgttt atttgatatg cggtttggct
     4021 atttatggtt tgatgcgggc catgggtgat ttatttttag caaatagtac atttaactct
     4081 atcagtgatt tcgttgacta ttatttaggc aaaaagtggg cattttttgt caattggact
     4141 tattggcttt gctggcttgg agctggactt gcggaattaa cagcaatagg attgtatatc
     4201 catttctggt ttgtacatgt accagctttg ataagcggag gtagcgcact aatgatctta
     4261 atggcagtca accttgttgc agtcaagtgg tttggtgaat tggaatcttt tttctcaatc
     4321 atcaagatct tggggatttt cctttttatc ttattgggta taggattgag tgtctcagat
     4381 tttcataccc agtctatcgg aatcaaagaa aattttggac agatcgatga attactaccg
     4441 tttggctttg ctggaatggt agtctcattt cctatggttt tattcagttt tgctggaatt
     4501 gaaatgatcg gcttaaccgt tggcgagaca agtgatccgc aaaagaattt gaaaaaagca
     4561 atcaactgtc tgccttggcg tattctcttt ttctatatag gaacgatttt gtcgatttta
     4621 tttgttgttc catggcattg tttagattta aaagaaagtc ctttagtgac tatgctgcaa
     4681 gtgatccatt gccaaggcgg agcagcaatt gtcaatctaa tgattttggt tgcttctttg
     4741 tcctcagcaa atagtgcagt ctacagtact agcaggattt tatatcaaac agcaaaagaa
     4801 aaaagtttgc cccattggtt ttgtgcgctt tctaagaaag gcgtaccttt acacggtatt
     4861 ttaattacag gaatgttatt ttttgccggt ctttgtctac atttttttgt agctgatagt
     4921 cgattgtttt ttcagctttt ggcaggcatg acaacgagtt gttttctatt tgtgtggtct
     4981 tcgatcttgt gtgcacatgt tcaatttatt aaagaaaaga aacggaacac tacacaaaga
     5041 tataaagatc ttgctttgtt ggtgtttttt acttcaattg ctttcagctt gttattcgaa
     5101 agcacgacac gctttattcc ttttttattg gttatctggt ttgtattctt aaattttgta
     5161 tataaaagaa taagcaatag aaatatcagt taggcccaga tacattttgc aaaacgaatt
     5221 atttcagacc cccctgaaat aatcgatacg cctgaaaaaa cttccctgcg tataataaca
     5281 gtatcaatta aacaggaggg gataggatga actggtacag attgacggta gaagatgtat
     5341 tgaaaaacta tcaaacatca acagaaggct tgtcgcaaga agaacgacag aaacgcctga
     5401 ctgatcaagg atataatgaa ataaaagcaa ggcagcagac aaaacgatgg aaaaaaatag
     5461 caaagcattt tactgatctg ctgatgatcg tgttgatcat tgcctctttt ttgaaatttg
     5521 cttctagtga atatattgaa ggaagtatta ttttattcgt agtgatcgtc aatggactcg
     5581 ttagttactg gcaagaaaga aaagcggaag aatcattgaa tggtttgaag cagctgatgg
     5641 gacaagaagc gatagtacta tcaaacggga tgcaagaaaa aatccctgcg aaagaactgg
     5701 taaaaggaga tgtcattgtc ttacgtgccg gagatgtcgt tccagcagat atccgattaa
     5761 tcgaagcaca tgatttatta gtggaagaat cgattttgac gggagaatca gaagcatctg
     5821 aaaaaagtca tgccgttttg acagaagaag aatcgatcgg cgaccagaaa aatatgggtt
     5881 tttcaggaac gcttgtacaa tctggttctg cagtcggtgt cgttgtagaa acaggaaaca
     5941 cgacagaaat cggtaaaatc aatcaagcat tgcaatcggt cgaacaacaa acgacgcctc
     6001 tgattaaaaa aatcaaccga ttaaacaaac aaatttttca aggcattctt tgtttgagcc
     6061 tattcttagt tatttttacg acatttcgat atggtatgga ttggcatatc cttctttcgg
     6121 ctacgattgc tttggtggtg tcaatggtac ccgaaggatt gccagctgtt ttgaccatga
     6181 ttctatccgt tggagtacat gaaatggcaa aagaaaaggc gatcatcaaa ggacttcctt
     6241 cggtagaaac attaggttct atgacagtga tctgttcaga taagacagga acattgacaa
     6301 aaaatgaaat gacagttgtg gatgtagctg caaaagaaga agcctgcgtg ttgtccatta
     6361 tgaaaaactg tcaggaatta aaaacaaaag aagaacagaa aacagaaaat ttaagtggca
     6421 atccgacaga agttgcgctg attcgacaca cagaaacagc caatctacct ttgaaaaaaa
     6481 tagaagcaaa gattccgttc agttctgaat acaaatatat ggcaacgatg catgcggagg
     6541 aaaaaggagc ggtgatttac gtcaaaggtg caccagaagt attatttgct aaatcaactc
     6601 tttcttctgc agaacaagaa gcatggagtc agacagctgc agaatttgcg agaaaaggtc
     6661 agcgtgtttt aggatttgct tataaaaaag tagattcgaa acaggaactc acgcatgaga
     6721 ctttaaccca attaactttt gcagggatcg ccggattgat cgatccgcca aaagaaagtg
     6781 cagtcaaagc tgtaaaggaa tgccaacaag cagggatctc agtgaaaatg attacagggg
     6841 atcacaaaga cacagcaaaa gcgattgcgg aacaaattgg cttaaaacat actgccaaag
     6901 ttttagaagg gatcgatttg gatcttatgt ctgatgaaga gttgattcag caagtgccaa
     6961 tagtcgatgt ctttgctcga acgacaccag aacataaact gcgaattgtt aaagctttgc
     7021 agaaaaatgg agaaatcgtc ggaatgaccg gagacggcgt aaatgatgca cctgctttga
     7081 aaagatccga tgtaggaatt gctatgggaa tcaaaggtag tgaagtaagc aaacaggcag
     7141 ctgatatggt tttaggagat gataatttcc atacgattgc aaaagccgta aaagagggcc
     7201 ggcggatttt ggataatttg caaaaaacca tcaatttctt cttgccaact gcattagctc
     7261 aaggattgat cttgatttgg gcacttatgc tgaatcgccc gctacctttg tccccagtac
     7321 agattttatg ggtcaatatg gtaacgacaa tcactttatc ttatgcacta gggttcgagt
     7381 cgccagatcc agaaatcatg aagcgcccac caagagatcc aaagcaagga atcctatctg
     7441 gttatcattt gtttcggatc atctatgtct ccctactgat catggttcct gcctatttat
     7501 tggctatgca gtttgaaggg caaagcttac agcaaaccat tcttctgcaa aatatcgtct
     7561 tagctcaagc tgtttacatg atcaattgca gagagttgtc caagccgtca ttgaaccaag
     7621 gtcttttgca aaacaaagcg ctttttcttt cattaggtat ccttgcttta ttgcaaggga
     7681 tggttatttt tctaccagtc ggccagcagc tgatcggtac agtaagttta acgatgacac
     7741 aacagttatt gattggagca aatgtgattc ttctattctt agttgtagaa atagaaaaag
     7801 ggatcatgaa caaactgttt cgcaaaaaag aaaaaacagc tagagaatac agtcgctagc
     7861 cgttactata aaaaagtttt gaaagtgagt tcagtcaaac gattgagctc atttttcgtt
     7921 ggaatagaag aaatgtatac aatagggata tctagagaat ctagagggaa attggaaatc
     7981 aagatatctg ttttttcaaa agaaatagtg gaaagatcgg ttagttcgat aggtaagaag
     8041 cagacacgtt ttcctaagta atcttttaat tcctgctgca agaacgcctt ccatgaaggt
     8101 tctccttgga aaataaaata gacattgaac aaattcggtt tgatagaaaa aatcgtttgt
     8161 tggatcagta gagacaagag ttggagaaag taatttggtt cattgatttc tatttctttt
     8221 tttagatcgt tcagtaaatt ctctttctgt tgatacaagt gtgcatattg ctgatcatat
     8281 gtttctaaaa aacgatggta ttgacggtcc aatgcttcaa tcaatatcgg cgattcatga
     8341 tattttaata tgaggagagc caagttatca gtcagagatg ttttttttag ttgatcaaga
     8401 gacagctctg aagctagttc ctctacaaac gtttcgagga cctctaaaaa tggagaatga
     8461 tgcaggcagc gagcggtttc ttgtccatag ttattgttgt aattccaact ttccagaaag
     8521 cagaaagaag caaaagaaag ctcagtgtct ttaagataaa taccttccgt taaatacaat
     8581 tttttcaatg cagagtcata cagggaggag agtgtgctgt tggtatggat agaagaatct
     8641 aatcggcaat tcgcttttat acggatcgta ttgataaaga accaacatgt tccgaaaatc
     8701 tcttctgttt caaccgctag ttcagtttga ctgaggtttc ttaaaaattg aaaataatgg
     8761 gattcatgga tcgaataatc ctcgaaaaaa tagttgttat gagtgaaagg aatcagcaat
     8821 cgatgataaa aagtacgaat cgcacgctct tttccaagaa tacaagctgt atatttcgtc
     8881 aaatcgatct gcaagtcgta acttgctaaa cgctgattga ttttagtcac atgtcttttc
     8941 aatgtcccat aagaaagccc atggtcggct agaaaatcag ggacagcaac tttttttgta
     9001 aaaagcagat ttttcagcag ttcgacactg actgattgtt tgaaaaaaat ctcccaaagg
     9061 tcatttgtta ataaattttg ctggttttgc aaactgattc ctgcttgatc gctgatgatg
     9121 tcccagcctt cgggcagctg agagcgaata gattgaaggt cagaaaaaat cgttcgttcg
     9181 gttgtcccta tgatttcagc taattcattt acggttatct ttgtatgatt cagcaattgt
     9241 tctactaaag aaacatgacg tcgaatatct ttttcggtaa tgattttttt cattagtgaa
     9301 tacatcaatc agactccctt ctattctaag aatacggaga aaataaacgg tggtcaagtt
     9361 tcaactgatt tttcaatcat tgtttatttt tctggagcat ttactgatga taagaatttc
     9421 atcatgaact tgaaaaatga taccactaaa atcaaaaaat aggtgggcaa acaataataa
     9481 acattttaat agtagatggt agcaagacca acacaggcgg attgaacatt taagagttga
     9541 taaaaaagaa aaatatcgaa gtatttgatt agcaactact tgtattcatt tttctattct
     9601 tacgtggtat ctttgaataa agcgaatcaa taagaaagag atggaagcag atgctaacga
     9661 aaaaagaact aatcgatcac atcaatgaaa catacaattt gtctccagaa tatattttta
     9721 aaaaatttcc tgattattgc gtattcagac atcaaaaaag caaaaaatgg tttggggtcc
     9781 tgatgtctgt tccagaagaa aaaatctatg atgatgcaag tgaccatagg atcatcaata
     9841 ttcttgattt gaaggtcgat cctgaacttg gcggaatttt gcgaggaaaa gaggggtatt
     9901 ttccagccta tcacatgaat aaagaacatt ggatctctat tgatctacgt aaaatagaaa
     9961 agctagatga actagcagat ttgattgatg gaagttttaa atcaacgcaa tgaatgtcta
    10021 ggacaataga gaagaggttg tgacagaaat gtttagcttc aagaaataag aaggaattta
    10081 cgaaaattgc ttctcaaatt tttgggaaat ttcggcttat tttctaagaa attacttctg
    10141 gaacaccgtt tgttcgaaaa aagagctgcg acaagaattt tgtcccagct cttttttttt
    10201 tattttccta aataaaattc ccgcatcgtt tttgcaatca ttttgatcaa aggatgatct
    10261 tctgtgaagc tagaagataa ataaattgtt cgtgtcaagg gcaaatcttt gatttcccgc
    10321 aagacgaaag gatcattttt atcgtaagac caagaaactt ttgggaaaaa gctaacagct
    10381 aatccttgct tgacaataga acgtaatgtc gcaggatcat ctgtcgtcga actgatcgtc
    10441 aattcgatac ttttcgcttc aagtgctgca tccagtgtat gacggaaggc gttgctcttt
    10501 gacagcatca agatgggggt gcgttggata tcttctaatt gcagatgttc ttttttagca
    10561 agaggatgtt tcttaggaat ggcgacataa acaggttctt tcatgatagg aatattggtc
    10621 atgccaggct tcatctccga agtgatgtac agtacaggat cggtaatatc atctggtaaa
    10681 tgatgttgca tgatcgttaa ttggatatca ggatgctttt ccataaaaat tcggaccaag
    10741 ttcggaatag aaacagaggc tacctctaaa tacaatatga cggaagtaac gatcttctca
    10801 ttgaccgaag acaataattt gattccttct tctaatgtgt ctaatgtctt gactgcatat
    10861 ggataaaatt cttctgcata aacggtcgtc tcaatgtttc tgccgctatg gtaaaacaaa
    10921 gggacaccta attcgttttc caaatgtttg atcgcacggc tcaaggcagg ttgagaaata
    10981 aataattctt tggctgcttg ggtaacattt tttgtttcga atactttttt aaaatactgc
    11041 agatgaagca atttcataat tatccatccc atccatacct ttttgttatg gttttttctt
    11101 tttttggcat tttttattat agcagaaatt gactaagata gcagtggaaa aagtaagtgc
    11161 tgaggaggcg aggctcatga gtacaggttt cttatttcct ggacaaggca gtcagaagtt
    11221 agggatgtta gcggatatcc ctgaaatgta catggaacgt ttagaaaatg tgacggggta
    11281 tcggttagtt gataaagaag aaaactatca agatacggtt tttatccagc tagcattact
    11341 gacaaaagca gtattttatt tagatgaaat ggaaaaaaac ggtattcagc ctgatattgc
    11401 agcaggacat tcgattggtg ctttttgtgc agctgttgct tgtcgatctt tgagatttga
    11461 gaatgcagcg gaactagtct atcaccgcgc agtcttaatg aaagaagctt atccaagcgg
    11521 ttatgcaatg ggcgtagtag taggtgtgac gagaagcgaa gctgaggaaa ttgtagcaaa
    11581 aacatttgat cctgaccatc cagtttatct gtcaaatgaa aattgtccga tgcaacatac
    11641 gttatctggg agtatcgaag gtttatccgt tacgttggca gaagccaaac ggcaccaagc
    11701 gcaaacagcg aagctgctga aagtccctgt gccatctcac tgtctcttga tgaatgatac
    11761 ggtcaagaaa ttcaagccgt atatgcagtc tgtccatcta tctgatgcta gatgtttgta
    11821 tataaaaaat acggatggaa gagccactca ggacgcagaa gagattcgta aagatttgtt
    11881 ggaaaaccta gctttccctg tgcagtggaa ccaaatgatg gacttggcaa aagaattagg
    11941 aatggatctg acgattgaat ttccccccgg caatactttg acaaagctga ttcatacaaa
    12001 atttggtgta gataaaggga tccgtacgat caatctagac caacatggta ttgatgacgc
    12061 cacgtttcta tatcaaaaat ggagatgact aattatggaa aaaccaaacc gcaactgggc
    12121 ttctaaacgc gaaaaaaaag cgcagcgatt ggaaaaaatc agcaacttgc tcaatggcaa
    12181 gtatgtagaa actgaaaaaa tcgtagaagt aatggaaaaa ctgattgctc ccggcgacaa
    12241 agttgtatta gaaggcgaca atcaaaaaca agcaagtttt ctttcagaaa gtcttgaaaa
    12301 agtagatgca gaaaaagtga atgatctgca tttgatcatg tctagtatct cacgaccaga
    12361 acatttgaat atttttgaaa aaggaatcgc aagcaagatc gatttctctt atgctggtgc
    12421 acaaagcttg cgtgtcgccc aaatgattga agacggcaca atgaaattag gtgatatcca
    12481 tacttatttg gaactttatg gacgcttatt catcgatttg attccaaatg ttgttttagt
    12541 agctgcagat aaagcagata aagacggcaa cttgtacaca ggtttcaata cggaagaaac
    12601 accaacaatc atcgaagctg cagctttcaa agatggtatc gtgatcgttc aagttaacga
    12661 agttgtcgat tctttaccac gtgtagatat ccctggtgac tgggtagacg ttgtcgtgaa
    12721 agcagataaa ccttatcaat tagaagcttt attcacacga gatcctcaaa acatcacaga
    12781 attgcaaatt ctaatggcaa tgatggcaat caaaggaatc tacgcagaac acggcgtaca
    12841 atctttgaat cacggtattg gattcaatac agcagctatc gaattgttac tgccaactta
    12901 tggtgaatca ctagggttaa aagggaaaat cgcgaagaac tgggtattga acccacatcc
    12961 aacgatgatt ccagccatcg aatcaggttg ggttgaaagc atccatagtt ttggtggcga
    13021 agttggtatg gaaaaatata ttgctgctcg cccagatgtc ttttttgtcg gtaaagacgg
    13081 cacgatgcgc agtaaccgaa cattatcaca agctgcagga caatatgcca tcgatatgtt
    13141 tatcggttca accttgcaat tagattacga aggaaactct tctaccgtaa caaaaggacg
    13201 tttatctggt tttggcggtg cgcctaacat gggacataat cctggcggtc gtcgtcattc
    13261 tacaccagca tggcaatcac ttcgcgacaa tgatgatcca ttagggaaag gccaaaaatt
    13321 agttgttcaa atggttgaaa catatggttc aaataaaaaa ccagtctttg tcgaagaatt
    13381 agatgcacta gctgttcaaa aacaagcagg cttagccaat gcaccagtga tgatttatag
    13441 cgaagatact acacatatcg tgacagaaga aggaattgct tatttgtaca aagcaaaaac
    13501 aaaagaagaa cgacaagcag caattgcagc aattggcggt gttacacctc ttggaatgac
    13561 tagcacgaaa gaatcactag atagcttgcg tgcagccggc atcgtgaaat taccagaaga
    13621 tctaggcatc aaacgtagtg aagcgaaacg ttcattatta gctgcacaaa ccatcgatga
    13681 tttgatcgaa tggtcagatg gcctgtatga accaccaatg aaattcagaa cttggtcata
    13741 aaattggcga gaaaaacctt ttgtcgccat ttacccagat ggaagaagaa agacgaataa
    13801 gcagaacgtg tatgctctgt tttacaaaaa ggggagagat ccatggaaca attagcttat
    13861 aaatatccaa cgaaacagcc gatcaataag aaaatccatg taggagtggt cggttcagga
    13921 gatttggaaa tcttgatgtt tccaacagaa aaaacagaat ctactgtaaa aatttgtaca
    13981 ggaagcgatg gcttcggcga agtatggaac aatgtattaa cgagattttt tgatcgttat
    14041 ccaatttcag cagatatcgt gatcaatgac ttcggtgcta caccaggagt tgtgtaccta
    14101 cgattgacgc aagcaatgga ggaactttca gatgaaaaat agttttgtag aattaaacgc
    14161 cagagaacga gtgaacgctc tacttgacaa tggacgtgaa ttgattgatc cattcgatca
    14221 aatcatcgcg ccaagtttac cagcacaagg aatcgtccct gaaagtgatg acgggatcgt
    14281 tgtcagccgt ggcacgatca atggcaaaca agcagtagta atcgctatgg aaggaaaatt
    14341 ccaaggtggc ggtatcggtg aagtttccgg tgcaaaaatc gttgctgcac ttgaacatgt
    14401 gttagaagaa aataaaaaag gaaatgagat ctttccagtc attgtactag atacaggtgg
    14461 tgttcgtttg caagaagcaa actacggact gttatctatt tcagaaatcg gaaatgtaat
    14521 cattgctcta aaacaatatg taccagtcat cggtttgatt ccaggacgtg taggatcatt
    14581 tggcggtatg tcgattactt cagcattaat gagttattta atcgcaacaa aaaaagcgag
    14641 agtcggtttg aatggaccag aagttattga acaagaagca ggcgttcgag aatttgattc
    14701 tagtgacaaa gacttgattt ggaatacatt aggatctcgt caacgggtag aatcaaaaat
    14761 cattgatgaa ttagtgactg atagtgtaga agcaatcaaa gctgctgtca tcgcagcaat
    14821 ggacgaacga aaagacacgc atcgcagtga aagctcagca ttttatttat cattacttga
    14881 ttctttagat ctttcaaaac caatggccat tgacgattac aatgaagcaa ttgctaacca
    14941 tcaagcaaaa tcaattgact taccagaagt agcagaagga aatgaaccag ctgcagcaag
    15001 tgttggttac gattggtttg cggcactaac tggcatgaaa aatccaactt cttctattag
    15061 tactgtgtat tatggtaaat caagtaaatt catagaagaa gccatcgtga catcaatcgt
    15121 acctgatcct aaaaacccga tgtaccgtgt acgaaacggt gaagttggtt tagttgaagg
    15181 tttccgcatg ggacaaattt tggatcacgt ttacgaagaa gataaagaca aaaaagtgaa
    15241 acggccaatc atcgttgtga tcgatgtacc aagccaagca tacggctata acgaagaatt
    15301 gatcggtatc catgtggctt tggctaatag tgctgctgct tatgcaaaac tccgtcaagc
    15361 tggacatcca gttattggat tgattgcagg aaatgctatt tcaggtgcat ttttagcaca
    15421 tggattacaa tcaagccgct tgattgcttt aaacaataaa gaaattaccg tccaagcgat
    15481 gtctaaagaa agtgctgcac gtgtgacaaa acgttcggtt gctgaaatcg aagcagctgc
    15541 tgaaaaagtt ccagctatcg cttacgatat caataattat acaaaactag gagcgatcta
    15601 tcatttccta gaagatatca ccgaccaagc tgcttcagaa caaaatgtca gcaaagtcat
    15661 cgaagcaatc aatgaagcca ttcaagatgt ccgcaataca aatgacacga tgctgacaaa
    15721 tcgatacaca aacgaaacag ccaaaacatt cggacgtgtc gaaacaaata aagtccgtgc
    15781 caaaatggac gaagaatggg atgcataaaa aatgattcaa gcacatgata ttgtcctttt
    15841 tgataaacat gaaatagaag ggttcacctt gcctgaatgg gtcgttctaa ctgaaaagat
    15901 gttcggaaca gttcgtcgtg cgtcgacgga tcacgcagaa atactaccga ttggtttacg
    15961 tggacctgcc cgcaatcaga gatggtcaac agaaatctca aaacatgccg tcaaacaagt
    16021 cattcatccg tgggacatca tcgaacaaga atcgtataga caagagaaaa tcatggatta
    16081 tcctgtttat cagcaatttc atgctgctag aaacatatta tcaggatgta aatggggaat
    16141 cggcggcagc ttaggatttg aattatcgac tgggattccg gcagtaaaag aaaccagtga
    16201 ttttgatttg ctgctgtacg caaattcacc gattgaattg ccaattcagg taattcaatc
    16261 gcatcctgct ttttttgagc aattcgatac ccaagtagtg actcggcaag gcggtttttc
    16321 actaaaagag tatttgagag agccagagaa aaaaattcta ttgaaaacag tcgaaggacc
    16381 taaattaacc aaagaacttt ggtaataaag aaaaagagag agaaagagat gtttgaaaac
    16441 aaaaaaatca aaatcaacgt aagtgacttc cagaaagtat cagctgtaac ggctgttatg
    16501 atgcaaacga tattgagctt tgctttggca aacagccaca gcgcaacaaa tacagctttt
    16561 cttggtacct tttacgtcat cgttaaatac acagcaccaa tgtttatttt tgcaattgtt
    16621 tataatatgg taaaaacaag tcagcatttg tcttattggg aatttttgaa agaaaagttt
    16681 tttgaactag tcgttccgta tcttctatgg acgacggctt atctttggct atttccagca
    16741 gtccaacaac aaactccgta tacggatact gcaagctttt tactgaaata tgtcacaggt
    16801 gatggagcgc ctcatttatg gtataccgtt atgatgctgc agatccaatt gttcatgccg
    16861 ttttttgttt ggctcggtta caaagtattt gctaataaaa aatatgtatg gccagtattg
    16921 attggttcaa cgattttata tgtcgcatgg tatctgttct atcaaacaca agtattgact
    16981 gggccttatc atgacagctg gtacttatta gatcggtttg ttgctagttt catgatttat
    17041 ggggtagccg catttgttta tcacgaaaaa gtctatcaat atttggacag agtacgttat
    17101 cttttcttac ctgttgcctt agttatcgcg ttcttttctg tccgttcatt gctcgcacat
    17161 ccaggagact tgagctttgc gaatgcacct tacttgaata cgatccaaag tttatacagt
    17221 ttagtcatca tctttgctgt cttcataggc gcttccaaaa tgattgtaaa cgattcgcca
    17281 aaattgccat tgttcaaatg gttgtctgtt tatgcttatc gtacgtattt agccaatgta
    17341 tttgttttcc aagtactgtt attattgttt aaagatagtt ggctgcagtt accttcagga
    17401 gtcatgattc ttgtagctta tctaatgacg gcaagttgtg catttgcttt aagctggtta
    17461 cttcatatca tttgggtagc tatcaaaaaa ggtttcagca agtaatagta ggaagaagga
    17521 gtgagtgtca tgataaatga cgaacgaaaa aaagcagtga ctcagcaact cgctgcgttg
    17581 gtagaggaag cattgatcgc agaagtaaca ttaagtccaa aacctggatt agtggatgca
    17641 ctggatgcgg gtgcacattc cgatatggac tatgctttat ttcttaagtc tgctaagacg
    17701 ctaactcctt tttttgaaga aatggctcaa atagcatggc atcatgctat tgatcaggag
    17761 ttaagagaaa gaattgctga gattgggcga aaagcggaaa aggcaatgct gattgctaca
    17821 aatggcatca atacccataa aggagcgatt tgggcaatgg ggctattgat cagtgtcatt
    17881 gcacaaaaaa tcagtatgga acagcctttt tctttatcga aaatacttga tgatgtcagc
    17941 aagcttgctg cttttcctga tacaaaatat cgtcataaaa aaaccactca tggtcatagg
    18001 gtgaagcaaa aatatggcgt aaatggtgca tatgaagaag cgatattagg gtatccgcat
    18061 atcaggcttg caattcaagc agctaaggag ctagataatg attcatatgt acagaaacaa
    18121 atgcatatgt tgttagtttt gatgagttcg ctagatgaca catgcgtatt gcatcgcagt
    18181 gatcagaaaa cgttagtgga aatgcaaaaa cttgctcatg ctgctagtca aagtgcttta
    18241 ccaaatcgtc catttacagc actgttgcac ttttgtcagg aaaaccaaat ctctcctgga
    18301 ggaagcgcag atctcttgtc tgcaagcttg ttcatgctga agatagagga attatggact
    18361 gccgacacat acatttgtcc gatgttactc aatgactaaa taagacaaaa ttgctaaaaa
    18421 aacagtttat gcatctggtt attgactctt ccttttagca aggctatcat aaaaatagtt
    18481 ttttgtaata aaagataacg aggaagacaa tgaaaaaaat caattattta gcaatcattt
    18541 tgttagtggg cctatgtttg tttgctctgc cgacccaagg attcgctgtc gcgggtggtc
    18601 atggtggagg tacatctagc ggtcgaagtt cgggcagagg ttcatttggt ggtcgttcat
    18661 atgggggcag ttattttggc agtcgttctt acggcagaga ttcttatggg tattatggtc
    18721 caggtacagg tccttctagt tatgagcaaa cgggatttct tgcattagct gttttgtcag
    18781 taggaggggt ttctgctttt tctatcgtca agaataaaag acaaaagaaa aggctgaaag
    18841 cggagatatt gtcccgcatg tcaggaacgg ctaaagaaaa gaaagcacag tttctggaaa
    18901 tccaaaagat attcatgaag atccaatccg cttgggaaga agaaactcct gaaaaagcca
    18961 gaagctgtta tacagacaag ctgtttgccg aacatcagaa agttctgcaa aaaaacaaag
    19021 cagacggtgt aagaaatcat actaaaaaaa taactattca ggaagtagga aattatcgac
    19081 agatccacga aaatagtttt gccatccgaa ttgatttcag atgctgtgat tattcagtcg
    19141 atcgaaaaga tgggcgattg atctcaggaa gtaagacaca acgacagtat ttttctcaga
    19201 tatggtattt tgatttttct gagaaagaaa aaagatggca agccgatttt attcagccga
    19261 ttatgctgga ttgacaggaa atatttatga acggagttgt gacaaaagcc ttgtcgcagc
    19321 tcctttttct gaataaacgg tgtgccagag atagcctatc ggcaataagg cacgattttg
    19381 taaaatatgg gaagcgattt ttcaaaatcg cttcttatcg ctcaaggctg aacatctctg
    19441 tcacaacctc tccactaaat ggtgtttaaa agcttgcatc tgcgataata agaacaacca
    19501 aacgaaaaat aggaagcact attttagttt agttgttctt attacttgat gcaggacagc
    19561 tttttcacaa cctctttttt gtatagatgg gcaaaatttt tgtaataata ggcgtatgtc
    19621 tttttcgttg aggagatgaa aaagcatatg gcttattttt tgtttgcttt gcttactttc
    19681 tttccacttc aagtggcaga aatatctact ttttattatg taagcggaac catatacttg
    19741 aaatcagaag gagatcattt ctatgaaaac aagaacaatt gaaatacttc acagaatcat
    19801 gacagcccaa tatccagtaa aagcaaaaca tctagcaagt gagttcaatg tcagtcagcg
    19861 gacgatccgt caggaagttc ttgaagcgaa tatctggttg agcagtaaaa agcttccgga
    19921 gatccgtaca attcgcaaca aaggtttttt gttgaagctg gaaaaggaaa agcaagctcg
    19981 attagaaact gaaataaaag atgtgaaaga cggctttttg aatcggcgcg aacgtatttt
    20041 tgatctagtt ttagccgttg cttacagccg aatcccgatc catctaaaca aaaaagaaga
    20101 agaatttcaa atctctaaaa gtacattaga tgaagatatg agacggttac gagcaacact
    20161 ggtcagttat gggatagaga tcgtcagtta tggaaagcaa ggactggttt ataaaggttc
    20221 agaacgatct atccgtacga tgatctatga ttttatcaat cagaatctgg gaatgaccga
    20281 cttctttatg aatcaagatg cacaattaac accggctcaa aaaatctttc tccattattt
    20341 tcctttggtg gaaatcaaaa aaatacagga gatctattcc aataagttgt tgaaacaaga
    20401 agatgatatt tataaaaaac aaatcctgct ttttacattg atctggatcc agcgaatcag
    20461 gaaacatgaa tttatctctg ccattaattg gaaaaataca gaaataaaag acagtagctt
    20521 ttctgcattt attgaccaca tgatcgagga atttcaattg gacaatgtcc cgcaagtaga
    20581 aagaaattat cttcgtttca ctattgagac gttcaatgct agagatatct ccaattcaat
    20641 cgagtgggta caagctcaat tactaacgat ccaattgatc caatttgtag agaacgagac
    20701 acacgtacct ttccatctaa aggaagaagc tttattcgaa aatctctata agcacatggc
    20761 tgcactgatt gtaagaatca agaatcatat ccaagtcatg aatccattga aagaaaacat
    20821 ccgaacaaat tatttcccaa tctaccgcgc agtcactcat tttgttccta cgatcgaaga
    20881 agtaattggt ggcaagatac tggaggacga gatagctttt cttgtcatcc acttttcgac
    20941 gatcgccagt gtaatcaagc gagacttgaa ttatgtttat aaatcagtcg ttgtctgtaa
    21001 ccatgggatg gctactggaa atctgttagc agagaatctg aaagaaaagt ttcctcagat
    21061 cgagattact gcagttttaa gttcaaaaga aatcgaatta gtagataaat tggatgtcaa
    21121 tctggttttc agcacctttc aattgaatta tcgaaataag ccgcttttag ttgtcgatcc
    21181 gattttaact gagacgaata tcttggttat agaagacttt ttgaaaagaa atgaagaact
    21241 acagcggtta gagcctaaca gtcatgaatc gacggagtta ttcactcaaa taatagatgt
    21301 catgaaagaa agtggtggcc gagtagatcg aatcatctat ggcaagttag aaaaattatt
    21361 cgaaacaaac aatttagaaa tcaacaaaag ggagatccag cctatgttaa aagatatttt
    21421 gacggacagc aatatcatga tcaaagaaaa agccactact tgggaggaat cgattgaact
    21481 agctgctaga ccattggtta aagaaaacat tgtcgaagag cgctatgtcg aagcgatgat
    21541 tcgtgcagtg aatgattacg gaccgtatat cgtgattggc aaacacatgg cactagccca
    21601 tgcccgacca gaagatgggg tcaataaatt aggagtcagc gtagctacga ttgagcaacc
    21661 aatcgatttc ggtaatcctg aaatggatcc agtaaagatt attttttgtt tggcagcagt
    21721 agactcctac tcgcatctga acatcatgaa agaactaatt gaattgatca acaacgaaga
    21781 gaagttggat cgattgattg aatgcacaaa tatcgaagat ttcaaacaac tgcttttttc
    21841 agtagttgag aaataataaa tgatacgggg gagaacaaag atgaacaaat tgaacatttt
    21901 attcgtatgc ggtgcaggtt taggaagtag ttttgcagca cagatgtctg ctgaggatgt
    21961 tttgaacaaa ttaggagtag aagccaaact ggaccattgt gatatttctt ccgcagtttc
    22021 catgaaacca gatgtgatca tcacagcaga aaattttcgt tcgcagttcg aaaagttcgc
    22081 agttgatcct aaaacggcga tgatctattt gaaaaatatt gtttcaaaaa atgaaattga
    22141 agaaaaaatc accccagttc tgcaagataa gggagttatc taaatacaaa aagaaaagag
    22201 ggtaaaaa
//
LOCUS       DAZKOU010000047        22170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000047 DAZKOU010000000
VERSION     DAZKOU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.047"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..329)
                     /locus_tag="ACG827_001679"
     CDS             complement(<1..329)
                     /locus_tag="ACG827_001679"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483823.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(343..600)
                     /locus_tag="ACG827_001680"
     CDS             complement(343..600)
                     /locus_tag="ACG827_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483824.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(795..1349)
                     /locus_tag="ACG827_001681"
     CDS             complement(795..1349)
                     /locus_tag="ACG827_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0483825.1"
                     /translation="MTRNVKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIE
                     AVKETSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQA
                     VRDYMTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVL
                     LQLKVDQEAQKIDAIKIYYVRSAY"
     gene            complement(1358..2449)
                     /locus_tag="ACG827_001682"
     CDS             complement(1358..2449)
                     /locus_tag="ACG827_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0483826.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2457..4667)
                     /locus_tag="ACG827_001683"
     CDS             complement(2457..4667)
                     /locus_tag="ACG827_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0483827.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4683..7211)
                     /locus_tag="ACG827_001684"
     CDS             complement(4683..7211)
                     /locus_tag="ACG827_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0483828.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7263..7673)
                     /locus_tag="ACG827_001685"
     CDS             complement(7263..7673)
                     /locus_tag="ACG827_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0483829.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7686..7904)
                     /locus_tag="ACG827_001686"
     CDS             complement(7686..7904)
                     /locus_tag="ACG827_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0483830.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7901..8743)
                     /locus_tag="ACG827_001687"
     CDS             complement(7901..8743)
                     /locus_tag="ACG827_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0483831.1"
                     /translation="MKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVAL
                     YMNTFDNHEQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTAS
                     YKVTYDIRSMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMI
                     TYDDTLYETVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEM
                     KVLRYKETKKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKG
                     EERK"
     gene            complement(8972..9883)
                     /locus_tag="ACG827_001688"
     CDS             complement(8972..9883)
                     /locus_tag="ACG827_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0483832.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9902..10246)
                     /locus_tag="ACG827_001689"
     CDS             complement(9902..10246)
                     /locus_tag="ACG827_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483833.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10266..10544)
                     /locus_tag="ACG827_001690"
     CDS             complement(10266..10544)
                     /locus_tag="ACG827_001690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483834.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(10903..11403)
                     /locus_tag="ACG827_001691"
     CDS             complement(10903..11403)
                     /locus_tag="ACG827_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483835.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11404..11949)
                     /locus_tag="ACG827_001692"
     CDS             complement(11404..11949)
                     /locus_tag="ACG827_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483836.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11946..13202)
                     /locus_tag="ACG827_001693"
     CDS             complement(11946..13202)
                     /locus_tag="ACG827_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0483837.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13563..13874)
                     /locus_tag="ACG827_001694"
     CDS             complement(13563..13874)
                     /locus_tag="ACG827_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483838.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13897..15372)
                     /locus_tag="ACG827_001695"
     CDS             complement(13897..15372)
                     /locus_tag="ACG827_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0483839.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15821..16273)
                     /locus_tag="ACG827_001696"
     CDS             complement(15821..16273)
                     /locus_tag="ACG827_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483840.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16275..16640)
                     /locus_tag="ACG827_001697"
     CDS             complement(16275..16640)
                     /locus_tag="ACG827_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483841.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16739..21022)
                     /locus_tag="ACG827_001698"
     CDS             complement(16739..21022)
                     /locus_tag="ACG827_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0483842.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21169..21408)
                     /locus_tag="ACG827_001699"
     CDS             complement(21169..21408)
                     /locus_tag="ACG827_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483843.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21498..21740)
                     /locus_tag="ACG827_001700"
                     /pseudo
     CDS             complement(21498..21740)
                     /locus_tag="ACG827_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21853..22104)
                     /locus_tag="ACG827_001701"
     CDS             complement(21853..22104)
                     /locus_tag="ACG827_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483844.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6478 a         4804 c         3308 g         7580 t
ORIGIN      
        1 gaattcattc cccaaaggcc ttttaagatt gatacagcag tccgttcact tcttttagac
       61 ttgtcataca agtcttttaa atgatcccat atcagactag tttgtctatc tagcttcaat
      121 ccattaaacg ctaatgaacg gttcattttt tctattactt tagataaagc tacagaaatt
      181 tttatacctt cacaacgcgc atcttggatt tcaataatag tatcatcaat tagtttttga
      241 atttcttgcc tatcaacata ttcaatctta gaaagagaat cttttaaagc tactaatgat
      301 tcaacgattt cttttttctc ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc
      361 atgtagagca gagtccattg aaccagtaat tacactatct acaacgccat tccaatttac
      421 tctacatttt ccatttttca gatttgtcgg tccatacgga tagccttttt gacattcaat
      481 gttcggatta taccagatag gattatacca ttcagccgca gaagcagatt cagtcgatac
      541 aaacaatccc acaccaacaa atccaataca catcacacta tagccaatta gttttttcat
      601 ctcgtatcca tctcctaaaa aatagaatag ttcttatagt tattcataag acatattttt
      661 tccgtttttg ttttatcctt tctgtcctat aacaactttt ttcaagtaaa gaatgacata
      721 gtttttctta aaaaaacagt ctgttttttt ctatttcttc ggtgtataat aaatatgagt
      781 gttcattttt tattttaata cgcactccgt acataataaa tcttgatcgc atcaatcttt
      841 tgagcttctt gatctacttt caattgaagt aatacaaatt gagtaacctt attcattgtt
      901 tcttctccaa ataacaaata ttgatccgga ttctctatat cttgtgtcac ctggtaaagt
      961 ttcccagttg ttttcatttg tacatgcggg tctacatcaa tactattgtc tttaatacaa
     1021 cgatctgtca tatagtccct cacagcttga ttgcgtttat aaacatctgt gaaattactc
     1081 cattttctag caaaatcttt taagaaattt tttgtcattt catctatttg catttgttgc
     1141 gctgtctcgt cttctaatga accaatttga ctgttacttt cagtactttg aagagttcca
     1201 cttgtttctt ttactgcttc gattgtttct tctgtcgttc tcaattgtgt tttttgtttt
     1261 tcagaagaaa accaatgaca accagtaaga cctatcaaac ataaccaact aaatactaaa
     1321 attatccctt ttttcacgtt tctagtcact gcctttctta tttcacctta cgaaccagac
     1381 tacgaatttt tgtttgattg tacgtgcgtt gataccgagc cataatccgt ccttgctcgg
     1441 cattctgttc cacactagag aaatgttgtc cgccatatga aacatctgtt ataattccgg
     1501 tgtgaccata gattccagga gacaatgcac cacctgcttg ccaattcacc acatctcctg
     1561 cttgtaaatc acttggtttt gggttcatga ttacttgcca accataggtt gtccagttat
     1621 aatcttcccc tattaacatc gcaaacatgt gtccagaacc cattaactgt gggccaccta
     1681 attgagatac ataccatgcc gtaccaccat aacattcccc attatttacg gatcgtccaa
     1741 tgatccgatc caaaaccttc gcattgcctt tactttttcc tgcaataagc gaagtttttt
     1801 ttgttggtag gctattccaa tgcttgatcg caaaagtatc atattccgtt aagttatagg
     1861 acgttatgat actcattgct cctcgataat acggactccc ttcggtaata cctgctgccg
     1921 catagcctcc acgacctaga atcaataaac aacttccagg atctttattg cccacacatt
     1981 gattaaagtt gctttggctc gccaccataa agccataata gtcataacaa gcttttaagt
     2041 tcggaaactt catatagaaa ccaccttcag aaccaccaat tccacgtgca gaaccttgtg
     2101 ggtagggaca accaggaaac caggtaatcc caaaaaagtt cgtatcgttt cttccagatt
     2161 gtgactgtcc ataagctgat tcatagccta attgagaaag aatcacactc ggtaataact
     2221 gatactttac agctgcacca actgctgctt gcgcatgttc atctgtgatt ggaccacgtc
     2281 ctttaatctc tgaaaaaatc ggtaatcctt cggtatattc cccattaaaa gcgacagcta
     2341 gacctgtagc ttctcctcca tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa
     2401 aaaagggaag aataaagaaa agaagacaac ctagacaccc tttcttcatt tcttcctcat
     2461 cctttcttcg ataatggttt ttggactgtt cgtccttttg gtaaagtaaa atcagttcct
     2521 ggttttgcgt ccaaaatcgg cagtgtttta ggatcctttt ctattttttc cgattgctct
     2581 gttggcttat ctaaagaaga aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt
     2641 ttactttcag acgaacgttg ccccggttgt tgcacagaac gatttcctga tagagtggct
     2701 accgacgaat taggaacaga gtattggttc aataactgct gcattgctgg ataactccca
     2761 tttttcgcct gatttttcac ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat
     2821 tgtggcacgg gtacttttcc agaaggcaac ggaatcgatt gaccaacaaa agcgatacct
     2881 ttatttaaca atggttttct ttgattattc ccttgttgct tactcaattg tgccaatgat
     2941 tgtacctgtt gttttggtct tactgaattc atggacgaat ttggaatcaa accatttgaa
     3001 agaccagaat tccactctgt tttcgatttg gtagaagaca tttcaggctc taccccatga
     3061 taacgaacat gacgaccact ataaggcaag gcttgatttg aacgcccacc tagttcaaag
     3121 ggattaacca cttgcctccc ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt
     3181 tgaaatccat gtatcgctgc agaagcgagt ggattcgttg ctcgatacac attccctgct
     3241 aatcctgcac ctttgattgc caaattttgt ccagcagttg atagttgacc aggcagttgt
     3301 ttcatttgag acaaagaatg acttgctatg tttttcgtgt tgtttatagt ttgatctgca
     3361 gctcctaacc ctagtttctt taataaatct ttccgtttta caatgagtaa aattgatagg
     3421 agtgctttga caaacatatt tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg
     3481 tctactatgt tatagatcaa aaatgccacc aaaataatca cactgtaaaa agatttttga
     3541 aacatcaagc ccaaaaagga ctttaaggta ttcatgagca tataatcaaa tactggaaca
     3601 aaagaagcga gtaatgaaaa aggaatcaca agtaataaac acaacaccaa catttgaaag
     3661 ataaaacgaa tcatgccaat cactacaagt ggtaatccaa tacttagatt aagcaatggc
     3721 accatagaac caacgactgc tttataaaca gctttagttg gttgaatata gacacgataa
     3781 gccttctttt tccctctatc ttctcctgca gcagtggtca cttttgtctc aatatcagat
     3841 aattcttcac ttgacgcttc cttggctaga aattcttgtg gttcaatctc tgcttgatac
     3901 aattcttcta aatttgttgt cccataattt aacaatatat acggatcaac aacagccgct
     3961 ttataataaa gttggcgcac tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca
     4021 ccattgacat cttttgtcgc caattcatct agactatttg tagcagaaaa aataaagcct
     4081 tctacctcca cggacaaatc atttaaccct ttggtaaaag cttctccttt actaggtacg
     4141 ttactaccat agccaaacca aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac
     4201 accatttttg cgtaatgttt ccctctaaat atacgaagag taaatagata taacacatac
     4261 aagacgacaa tccccgcacc aataggatcg tatatttttg aataaatagt atccgaaaaa
     4321 gtgaaaaact ggtgaattaa ctcgttcatt gcagctcctt catagagctt ctcaatgaca
     4381 aaatcaaggc cttgccaaat caatttactt aaactaaaga acatattcgt catcattttt
     4441 agaaaatctg tgccatttgc cccaaataac attttgccta atgaaaattc actatcagat
     4501 aaataagaag aaaaggtatc aatatgaaat ttcccaatct catagatcga ttgatccacc
     4561 tgcttttctg ccgcatacac caatccacca acaaaaagca gtccgaatcc aatcgttaaa
     4621 aaaaacaaaa cgcaccattt cttttcctta tgctttatat aagccattgt ttcacctcat
     4681 ttctattgca tgattaacgt atcctgttct tctacttgag agtgacttgt tttttccgtt
     4741 gtttgtaatg cagcggtcat ttcttcaaac aaactgtgta cattgagttt tcctacacgt
     4801 ccatagggat ctaacatcca acactgtcct ttaatcatgt ggctcatttc agctaagttc
     4861 gcttcattta cttccaaacg taaatgattc aaaatctttt ctcgctcact gtcttcatca
     4921 aaagcaaaga cgacaccaaa attccctttt tctttatctg aacctaagtc accaataaat
     4981 tgtgtatcta acactaattg attcatctgg gaacgcccca cacgtgccat tgctgcaata
     5041 atactacgtc ctgcgttcga attattaaat acccatgctt ctgtaaaata aatcgctgtt
     5101 ttcttactac tatctcgttt tccaaacatt tcacaaaaac gacctaaact aatcatcaaa
     5161 cacaaagact ttcgatccgc atctgtatat agttctggtc ggacattgtc atttggtaat
     5221 ttcaaatctt ggatctctaa aatcgttgtt ttggcattaa agtctaatcc cgggttttct
     5281 ccataagaaa atcctaaacg taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca
     5341 tattctcgaa cctctttttg ttccatttgt tctaaatagt cgaccacatg catcaagcca
     5401 acttgttgtc cggcttcttt ttgaactaaa acttcttgga ttccctttaa taccgcccct
     5461 tttaaaaggt gattggtacc taacggactt aactcatcaa tcatatcctg ggctacttgt
     5521 ttcgcatcta ctcgggataa acaaacaatt ggatctaata caccataatt acggggattt
     5581 gtagcatcta acgtgacata actcattttt tgcaaatgtt ctacaaacaa agggtaatgt
     5641 ttctgataat acgggtcttg aatgagtttc gtaaaccatt tgcgcttttc tcctttggga
     5701 tccacaaata aactttgtac attcataaac gaactataca aaaagagcaa gccaactaaa
     5761 aaggtcttcc ctttaccggt ttttccagtt actgctacat gaggcgcatc aaacaacgca
     5821 cctttgattc cttccgcaat ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa
     5881 attaattctt ctaatggtgt tttttctcct cgagaaccac catcccctaa tgtatcttgc
     5941 cgaccaatat accagccgga ttccatacct aattctgata ctgctgaaaa caaccattcg
     6001 ccaactgctt ctacagttgt tcggtgcttc caatgtaact gccgtcccaa tggttgccct
     6061 aataaacatt tatagaacaa ttctcgttga tcattttttg cacggtggat atggataatc
     6121 gtatgaagtt gttgaataac cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg
     6181 tatacaataa aacatgcgat ccaatcgacg acaggttgcc cattatctac tcgttgcttt
     6241 aaatgtttta ctaatcctcg aacagttaat aagcgttcac taggttcttc atcattttga
     6301 accaattcct tatgctctgt tttgaaccgt tccgatagcc aattcagttt taaaccaata
     6361 cctccctgtc ctttcaagcg tggatagtga attttcattc gcaattctgc tgggaaagaa
     6421 agatcctgag caaattgaaa tagatcacaa tttctcatat tttcaggtgt ttccccaata
     6481 ggcaacaatg ctaaataact actccctaac tcatcttcta attgaagaag gcctttatgt
     6541 tccttcggta tacgtacata cgttccttga ttgtctttca gtacttccca attattttgt
     6601 tcttctactt tgtgagccat tcctcgaata tattgcagac gattgagata caacagctct
     6661 ttttttacta agggacgaat agaaaagcta taaagtaatt gtgtcacttc ttttacataa
     6721 ggatcacttt cagaaaacaa agtttccaaa tcatctactt ctaatccaaa taagcgtgct
     6781 aaacttcctg ctaaatctaa ccagattaga tttattacat catgaaaact ctccaccgtt
     6841 gctttttctt tgactttgat tcctaaaata aatgaaggac gatagacttg accttctcgt
     6901 tccaacaatg tcactgtttc ttttgcataa tacgcaccaa ttgcttgact atcgggagaa
     6961 aatccctttc ctaatgtgtc aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc
     7021 aactccacat cttcataatc tgctaaaagc aacagtaaac gagccatcat ttcttttatt
     7081 tcctgtgtct tttttggtgt gttttttgaa attgttcgtg gaaaaacatg atagtaggcc
     7141 cagatacaat cctctccatt taacaaacaa tttttcttga atgcttttac tggttctttt
     7201 acaagcacca atgaatttca cctcccaata aaaaaggaac aaccagttga ttgtcccttt
     7261 ttctaatcct ctaaataaac atacttattc acaaaaagta attcttccaa tgcattgttt
     7321 gataccttgt cccagttata atacacacaa tcaccattca tcatacgaat catataacaa
     7381 aaccaatccc ataaataaaa atgcactttt tttccatcag gttttagttt tgccagtgct
     7441 aacacacata aataggggag tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa
     7501 atgacccaac caataaatga acgtaaaaat aaatattcaa agattacgac caacaaaaaa
     7561 gttgctgcat tgactaatgc aactggaaat ggaagacgcc aattgcttgt aatctgttgt
     7621 acgcgataag gttccgcata aacaatcgaa taatcataag gttcccaatc cacttcacta
     7681 tcctcctaat ccataaataa tttttcaatg attttaccaa acgcatcaaa tacacgtgtt
     7741 ggatcattta agaagaaata aactgcggca cctgctgcga tagatattaa tgcatttcct
     7801 acttttgatt tcataaaatt tcgcaatccc agataaacaa ccaccattat taaaaaatag
     7861 ccaccttgcg ttaatataaa atctacaatt cccttcagtg tcatttccgt tcttctcctt
     7921 tcctatccaa ccaattatgt gttaattttt caacataata ctgatttgtt tttttcgtaa
     7981 tgacaatcgt catttgttct ttttgtaaaa gtgacgtatc accatctcga aagacgaccg
     8041 ttgcttttac aataaaaggt tttttcgttt ctttataacg caatactttc atctctactt
     8101 gatcaacttt agctgtttga ttgagtcctt caggttcttt catcatgtat gacatatctt
     8161 ttgcattgtt tttcgcatac ttatctaaga aatcttccac aaatccttca acatctttcg
     8221 cttccgacgc atcaaccgtt tcgtataatg tgtcatcata agtaatcatt cgttgttttt
     8281 ctgtattctt tggtagtgcc gtaaaataag gataagcaat aatacgacag cccttgtttg
     8341 aaatagaaaa gggaagattg agtacttgtg tgattgtcac acttgtttct ttcttactct
     8401 ctggattcat cgagcgaata tcataagtta ctttataact agcagttgga atggtttcaa
     8461 tttcttttaa tccaataaac gaagcagcag ttaattttcg aacacctgaa acacttcctg
     8521 gttcttgaat aaagttttgt gcataaaact ctttttttaa tgtttcttga cgttgttgtt
     8581 cttgttcatg attatcgaat gtattcatat acaaagcaac aaaacgattc ataaaggatt
     8641 caatttcagg tgtgtacgtt aattctgacc gactttgtac ttctttcttt tgtaattgtt
     8701 ggatttcatc tttcaaaaac gacacttgaa ccttcgtttt catggattga aaaaatgcca
     8761 agccccctga aaaacaaaga aataaaacca aacaccaaat ccatttcact gctttttttg
     8821 aatggatttt cttttgcttc ggtactttct ctgtataaac tttttctttc cgccgtagtt
     8881 gtcgtttttt cataagctac tccttctagt atgtttttac tagtacgtac gattgacaac
     8941 gataagcttg tttttctgaa aaatagtcca ttcaattacc ctcattttct attccttttc
     9001 tctttctttt atatccaaca aatccagcaa tacttgtcaa cactcctcct agaacagaaa
     9061 gtgcaacatg tatttcttca ccagtctttg gtaagacttt tgtttttcca tctgctgttt
     9121 tgatttcaat attgccactt ggaagtttct ttacaggaat cgacaaatcc tttaccaaat
     9181 tcccttcact atcttggatt aatggaatac catcttttac accaacaatt gtttgatcac
     9241 ctgctacttt taatggggtg gttggtgttg gaatatgggt caattcctct acattactag
     9301 tgacaatcgg tacactcatt ccttgagaag tattcgtcgt aatttcaccg cttggggtta
     9361 ccgatactgt gacttctttt gctggaacaa taggtttatt ttcttttgga attgcttctg
     9421 atttttcaac aggttgtttc ggttcttctt tttgttcttc actggtagac gacatcgttg
     9481 gctgttccgt acttgacgaa tcagtattct tttcagaaga actggttgtt cctgattgtt
     9541 ccggagttgt gactatactt gaatccgttg ttgttgaact atgcacacct tcttcttcga
     9601 tcgaatcagt cggtaaagtt gattcaggaa actctggttc tggtgtcact ggctcttttt
     9661 caggtttctc tgactgcaat tgttcaggtt ctgctggttt ttccgttgtt cctccattta
     9721 gctctgtctc aacatctggt aatccttccc ctggcaatgt ttctacaatt tctgacttag
     9781 gtgtttctac agtttcatct gcataaacca atgaactccc taattgaaac gaaaataatg
     9841 aaattcctag tgttgttacg actgctaatg ttgttttttt catgattatt cctccaattt
     9901 tttatgcaat tcgtgttttg ttcatagacg ccgcactaat attatctgaa cgataaaaga
     9961 actcccgtcc gttttcttct aacccaacat atggattatt tttgaataat tgaatcaata
    10021 gttcactggt gttgtatttt ggatagtttg caaaaaaata atactgtgct tgtcgcccat
    10081 ccgaaaattc aacaagtaat tcataacgat accgcttcaa tccttttcgt tgttttaaag
    10141 gatctctata ccaacttgtc catcgttcca ttcgtggata gagatagaca cgatacacat
    10201 agaacaatgc cataccacat accaccaaaa ataataatcg ccacataaaa attccccctt
    10261 ttcttctacc tttcttgaat catcgaacat tcataccctc tatagagatt tgaaaaatcc
    10321 atcatcaatt gttcatataa acgttgttgc aaatagcgat tgtttcgaat acataattct
    10381 tcatagccat caatcacttg atcttctttt tcatagcatc ttgtagctct atgacgctca
    10441 gcttgcatga cataaccaaa ctctgcacca tatcgttcta ctgttaataa ctgttgacta
    10501 cgcaataatg caatataaca atcacaaaaa tgttcaaccg acatttgatt tttctcacgt
    10561 ttgtgaaata ggtattcttt agaaagcaca ttattcgtta aacaatctga taaatagcga
    10621 taatctggcg gtcggttatt ttcatctgtg aatttttccc ataataaact gatttttatt
    10681 cgttcagcgg ctagatagtc ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt
    10741 ttatcacctg aataaacaaa tccatataat gcgaaacgaa ctcttccttt tcaattggct
    10801 ctcctaaaaa ttccagtgac ataccatctt ctccttccta aaaaaaataa aaaagcagaa
    10861 accgatttgg cttctacttt tctttccttt tatagctctt ttttacggaa gaatccgcaa
    10921 atgacatttt ttcaaaaaat caatcggcac aagttcacga aaacttggag caattttgga
    10981 tagaatcact tccccatctt gtaaaataca atcataaaaa tcatcagtta cttgatactt
    11041 acgcttctta caataattga taaaatagta gcgatcattt tctatttcca ttcgttgttc
    11101 tactaagcgc atacgaacat catcatagaa ataagccaca aatcgccctt caagttcatg
    11161 tttttcaatg ggatactctt ctggtaacaa tctatttttt cgtcgtttat acattgctgg
    11221 ataccttaga agaagttcat ctaaacgtct gtcagtatat ggtaactgac gaatcaacga
    11281 ttctaatcgt tctttataca gatagaataa ttggcgcaag caatagtgta ccgcctctgg
    11341 attttctaat actgttggat cacgtgacca atacgcacca acataaatca taattttttt
    11401 catttagaca atcacaccag caatactaat gaccgatcca caataggtag aacaagtggc
    11461 atagcgacca atttcctcta attctatctc aatctcttct aaagaaagat atgtctgttc
    11521 ttctgttggc tggcgactca tttcccaagt gacagtcacc aatgaaattt cttccataag
    11581 aggtttttcg ctgctaagtg ctgccacaca gttttcatat atcttcattt tatcgcagcc
    11641 ttttttaacc gctaaaatcg caggtattgg caatgaaaat cgataaaagc gaatgaattc
    11701 tagccttcct ttccttcggc taaattccaa ctcttcgagt tcttgttcgt atcccttacg
    11761 aacttctaat aagcgtgcaa tttcttgcca gttctctttt tcctgataac gtccagcttt
    11821 taccgcttgt tcaatataag gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc
    11881 atttaatgct tcgattgcag cctttaaatc caacaggcgc agccattctg ttgtcgtata
    11941 tgcattcatg caattttccc tcctgacgtt cccatccatt ctagatagtt ttgttgctcg
    12001 tcatttagtt cggcattatc aatcccttct tgtacaaagt ctcttcctaa aatacgatct
    12061 gcttcttgta acaactttaa tgtcggagct acttgtttca aaaaccagcg ttctgaacga
    12121 tctaatgaga atttttctgg ctttactgta aaatccaatt cttccatcga acgaaataat
    12181 tttgcccatc gtttatcaac aatccgtgca tcactttcca gtctatccca aacaataagt
    12241 tttgcattaa ttaaaccaac gccaatctct cctaaacgtt tcggtgaatc acctaaaata
    12301 aatgcttcta ctgcttgtgt tgctttctca ttggctaacc gtaactcata tcgattataa
    12361 ataccatatt tcgttaatgc ttctaattct gaaatttttt ctcggttcgc aatttcaaaa
    12421 cgtttctcat aaaaattaaa aaagagagga ctctttcgcg atccaaaata caaagataat
    12481 ccttcacttc gaaaatagcc atcatctaaa aaacaaccgc caatattctg gaatctctta
    12541 aagtccattt ctaataaacc tgctttttgt ttttctagta attcaaagag atcgaaatgt
    12601 tcttccgttt ccaatcccca aagttcatct aacgcaatat cgaatcggct acattgcaca
    12661 cgtgtataac cagcttccaa ctttccttga tttttccaaa tggaatatag ccatgttcgc
    12721 caatctgttt gttgttcacg catgacttcc tcatattccc ggcaaccttg tcctgaaaat
    12781 tgcacaagta cattttctgt tacagttcgt tttgaatgcc agaacaccca gatattccca
    12841 cgaacagaaa gacagtcaaa aagatacaag cccgttggac gtgtttcaaa ttctgtcaga
    12901 tccattcgta atacacgttg aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc
    12961 aaataatcaa tcattgctcg aagatctatt ccgcttttca tatacaatcg aaagcgttcc
    13021 ttgattcgtt gttcaaaatc ctctgggatt tttcttttcc cttgttccat ctttctaatg
    13081 taatcttttg agcagcccat caattgcgca aatcttgctt gtgatagcgg ctttttatct
    13141 ccacttatat tatgaattgc acttaacttc tttctgtagc tttttagctc aatcggtgtc
    13201 attttctcgc tccttttata aaataacaaa atcgattttt tttaaccctt tagaaacacc
    13261 gatatatcaa ctagtccccc tcgttttttg ggaaaagtag tacaactgct gaccttagat
    13321 aatcacttga tacaaaaggg tttataaaac ttttttctat gtgttatgtt atttctgccc
    13381 cccatattat atatcggggg gctttacaca aagaggccgt gcgcaagcgc acgacctctt
    13441 tgtgagctgc acacgctgct agcgagtgcg cattataggc ccatgcttcg cagccttaat
    13501 gcgcaccgcc aggcagagtg cggctaccag actacgtcta gagccctccc gatagataat
    13561 aactacaaaa ttaaatgatc taattttgtt atttcactat atttttcagg agaaaaaacg
    13621 cgataaattg acaaagttaa atcctcttct aaatctactc gataatgata caatcgagtc
    13681 aatcgttcta tatctttttt cgttagcaat acttgattaa taacttttcc tgtaaaaaca
    13741 cgttctttgc gcaaaaagcg gtaatcagtt acttgttcaa tacgattcaa ccaatcataa
    13801 aacgttctgg aagaaagaga aggattaatc attcgtattg ctgtagctac tctataataa
    13861 caactctctg ccatttaact tcacctcctt agttgattac aaaatatgcg catcagaaaa
    13921 acttgttcct tttggatctt ttgctaaata catttggcgc tctagttgtt cttgtaacaa
    13981 ctgattatca tctcccatcg tttgatcttg actatcctct tttactgtaa aatcatagac
    14041 aatcatttca gtaaatttct caaattcttt gataaagtga taatctttgt aaacaaatgg
    14101 cgcaaaaaac tctacaggta catcaaatcc tggaaaagca tatccacgac caggttgatc
    14161 tttgttgtaa aatggcttat ttttaaaatc ttctccaaaa gtcattaaat aaccaatggg
    14221 agacaatcga ccaacagaaa tacggaatgt taaattatcc cgaacaccac ttggaaaata
    14281 ttctgcatct ggtcgttgca ttgctaaaat aatgtacatc cctgcttgac gaccctttaa
    14341 tactaatggc tgaatcaatt tatcgactct atttctcgct tccatatcca acgaaccata
    14401 aaaagctgcc cattcatcaa aaaagataaa atgcggtggt aacccataat aagcataatt
    14461 tttccctggt tcatatttag gatctttcat ggttttcata taggcatacc gttgattcat
    14521 ttcttttaat gcatttcgta aacaaataat catttcttca tttattttgt aaaacacatg
    14581 tcctttgaat acatcgacgt cacctaatgc tgctaaatct gccgcttttc catcgcaaat
    14641 atctactgtc ccaacactta aacaagcttg aattaaagaa tacaataaat aggttttccc
    14701 accaccgatt gcaccagcaa tcaacatatt tggtgattga tcatataccc aatccaaagt
    14761 tttcattaaa tgaatacgtc cattttctgc tatacattga tcaataggaa tccgaccaga
    14821 atctgaaagg aatacataat tgattcgggc tttttctcgt tcttcacgaa ttaaatcacg
    14881 caaaaatact tcagataaca aatgacccat ttttaaaaat cgttcgtgaa aacgcccacc
    14941 atctaaagga aaagaaacca ctaattcttg tttcttttga taaaaataaa cttttggtaa
    15001 tctcattttt tcccttgttt gtttctcact aacttgtttc tttgtataaa caaaccgatt
    15061 gtcaattaac attcgaacaa gtaactgacg ccaataaaca gaggcaaaat atccttcttt
    15121 tagtcgcgac caatgcatca accctaaaat taacccacta accagcaaca aatacaacac
    15181 aatacttatt ccaggaataa gatacatttg ccaattcgaa aacgtactta aacgtggata
    15241 aaatactttc caactaaacc aacctaacag aatcaaaaat ggaaacaaaa agaaaccata
    15301 atagcaaaac cataaataac gattgaaata gcgaattcgc cgaccacgat acttgaaaaa
    15361 atgagctttc aattgtcaca ttcctccttt ctgtttttac ttttcctgtg ctaaaaactt
    15421 ttgacaaaaa gcaactaata caggaatatc acatttagta aaaaaacata atcgaaaaag
    15481 atttaatcga tttttataag ggtgtgcaca catattacat attcgtacta tacgaatcca
    15541 aatcacttcc tgatgatctt gtttccactg gtacggtaat gaaaaacgaa caagatcacg
    15601 taaaaacata cactgcccaa taaccgcaca aatatatgca tttgtttgta caaaaatcgt
    15661 tggattgtct ccaaaatact caatcaattg ttggatattt tgataacaag cacaatctct
    15721 caaagtattt gattggtttt atctgaattc aaaatctccc cttccttcta tcttagataa
    15781 aaagaatagg aagactcctt cctattcttt caaaaaaacc ttattttttc tctttttcct
    15841 tagaatcaac acttggttgt ttttgatcta aacctttttc ttgtgattgt ttcatattag
    15901 gattatttgt tggagcttct actttctcta atcctttcgc ataaatcact aaatctgaat
    15961 attcacgatt gccatcttgt gctggaatat ataccacatg atattctgga tcaattaatt
    16021 tcacaggatc acgtcgttta aaaccagtca aatctacatc tgctggaaat ttaactcgtc
    16081 gtgcaatctt tggttcatct ggatttccaa aaactaattg tgctcgctgt gatcgaattt
    16141 ctcccgtata atcgcctgtt tcattatcaa atacacgatc agactcttct aatgcgccaa
    16201 agcgtacggc tcctaaacaa gtttcatcta atgcttctgt aatgtcataa ttatttggaa
    16261 attttaatcc catcttacaa aacctcccca atttttacac cagttaaaaa gacaccaaca
    16321 tcttgatagc cagttgttcg acgctctgtt tgtcccatat catttgtttc acgactattc
    16381 actcgttctt tacgaaaaac aaaataagct tcttcaaatg aaatacgcac ctctttttct
    16441 ggtaatcgta atcttttatc ctcttcagct gtgagataaa caaaaaaagc aggctgttct
    16501 tctcgcgaac actctgcttg taattgaaca tatcgcccag acacaccatt aatttccttt
    16561 ggtggattag gttcccgtga acccaataaa aatacttgac ctaaaaactc tttcttcata
    16621 cgcaatgctt tttctgccat aaagtttttc ctcctcttaa aaaccaaaat aaattctaaa
    16681 tatttctaca ttacaatcaa acaagcggtt aaaaatccta accgcttgtt cctcatgact
    16741 aatctttggc tttacgtact ttataaagtg ttatacctaa agcaatgact aatactacta
    16801 atccaagaac cataagtata ctacttgttt gttcacctgt ttttggcaac attggtacaa
    16861 cagtagctgg tttaacccgt tgttctggtt tcactggttg cggtggtttt ggcgcttcca
    16921 caatttgaac cgtttgatct ttgtttgtta gatctacatt gtgtttcgcc acttcttcaa
    16981 acgtttcttt ctcatcttcc gttttcgctt ggagccattc cgcaaaactc aagccttttc
    17041 ctgcaagatt gcgaccgtcc actaagaatt caaataagaa ttccatcatt tctttatcgg
    17101 ccgcaaagac tttttccgca tgagtgcctt caaatggtgt gccgtctggt gttaagtagc
    17161 cttgaacaat taagcgatag atttgaccaa tcgcaagacc gtctactttg acccattcaa
    17221 gcacagtttg atttggcaag gccaagatct cttttccgcc atttttattc gtcgctttcg
    17281 tcgtaatggt tggattactg attttgatgg tttggtcatt gtcggtaata tctgcgtgag
    17341 aagccaataa tacgccttct ctaaacagat cttcaaagac cacgacttct ttgcctcgca
    17401 acgcacgtgc gtctagaata aagtctagat tgactgtacc atccgttgtt tctgccacaa
    17461 acgttaaggt acttgtgact tcttttccat ctactagcac tggtttttcc gttactttgt
    17521 ccattagaac accgttgact gtgtatgttt ttccaacgac caaatcgtgg tatttcacag
    17581 cgtctgtaat ggtgactttc gttaatggat taacctcttt caagccagtg ggtttatgtg
    17641 tagccgttgt acgtacactt gggttagtta ctcgtaccgt ttgccctttg tcgttgatgt
    17701 ctttatggtt tgctagttct tcatcatttc taaaaagact ttcaaacacg acgatttcac
    17761 gaccatttat acctgtcaaa tcaaaggtaa atggtacgat caccgtttca ttttctgctt
    17821 tggcaataaa gccaacactc gtttctaccg tatttccatg cacaagcaag ggttcaccgg
    17881 ttgctttatc cattaagatc cctttgacca agtatgcttt tcctggaaga atctcggtaa
    17941 tttcaacggt atcgtttaag gtaaacaatc ctgttgggtc tgcgactgtc aatccgtctt
    18001 caaagacagc cgtggtatga acggttggat tattcacttt cacactttgg gcttcatcgt
    18061 tgatatccgc atgtgctgca acttcaatgc catcacggaa aagtttttca aaaacaacga
    18121 tcgtttgacc tttcagaaca ctagcattaa ccacaaattc aaggtctttt gtgccattga
    18181 ctttgtctgc aataaaggcc aaggtacttg tgatttcttt gccatcttgt aaaattggcg
    18241 cattcgttgc cttgttcatc aatactcctg aaatggtgta ttcttttccg acaaacaagt
    18301 tggtataaga aacactgtct ttaatgacga tcgtttctaa tggatcaacc actttttcac
    18361 catcggcttt atttgacgct tgtgtttcta gttttggtgg aatcaagtaa ttacgtacag
    18421 aaaccactaa cgtttcccca tcatctggcg cattttgttt gaatgcaacc gtgaatgctt
    18481 gtttatctgt atttggttgg aaactttcta atggggcatt ttccaagaag taatagtcat
    18541 cggcaactag acctttcact aaaatttccc cgttttgatc agtggtatat tcaccaattg
    18601 cttgattttt accgttatac aaggtaaagt tcacacctgc gagcggcgtt ttcttcttgt
    18661 tccattcttc atacttgaat aattttacac ttttccgtgc ttgacggttc gcaaaatgga
    18721 tcactttgat cggttctgta tcattggtaa tcgagaacac taatgggttt tgattcaaaa
    18781 cgtaatttac gggggctttg atttcttcca aacgatagcg atcttttcca taagtaagtg
    18841 taccgttagt taggaaaaag cctttttcat tggttttaaa gatatccgtt gtttctgtat
    18901 catttggaat ctccattgtg acataacgat tggctaaagt atcaaaaatc ttgaattctg
    18961 tattagcaag tggaatcact tttcctgttt cttgatccac tttcaccact ttaagacgtt
    19021 gttcaattac tttatcttct aaaacataat ggaaagtttg cccattttca gaaattgtga
    19081 cttcaagtgg ttcaaatagt ttataccctt cttttccttt ggtttcagaa agaatgtacg
    19141 tatcaaagac aacggtattt ttaccaaaag tagcataacc atcattgtct gtcacaacgg
    19201 ttttcaccac tttgccagtt gtttttgacg tcaaagtgat ttcaatgcca gctaattttg
    19261 gtttgttatc tggattcgtt gaatcgacta aatctttatt agcaactttc actaaatcaa
    19321 tggttccttt gatcacttga tcttttgcgg tcgcattgac agtttttcca ggttcaacaa
    19381 caatttcaat tgcttcatca gaaagcacat aaccttttgg cgctttgatt tcttttaaaa
    19441 catatttgcc aggatcaaga tctttaattt ctgcctgtac tttgccgtta atttttttca
    19501 aggtaatttc tttaatcttt ttcccatctg cttgatacaa accatagacc gcattttcta
    19561 aagaagctgc accttgtgct ttcacaccag tttccgcatc ttctttaaac acacgaatgt
    19621 ttccttgttt tagaatttgt aaattaataa cagacgcatt aggttctgtt ggatgtaaca
    19681 caccaattgt ttgagctcct ggacgacgat aggctaatgg cgcaccttga actttaattt
    19741 tattcaattg aatttttgct ttatccggtg cctcttttgt gactgtaatc catacttcat
    19801 tgccgtgaat agatacattg acaccttttg gtttttgaat gtctttcaca tatttcaaaa
    19861 aatcattttt attctttact ttcaacgttt cgccaatttt taccgtatgt gtttgattat
    19921 taaaagttgc tttttcacca aagttattaa tcaaacgttg aatttctgct tttcgacgat
    19981 cataatcaac acggatattt ttgattggga tttttgtgcc caaagcttcc caaatcataa
    20041 attgtgccgc aatgtattgt tcatcggatt tgtctttatt tgtgacatat cctaaataag
    20101 agatcaagct tgtttttttc cgtacatttg aatttttcaa atagtttttc aaagcagtag
    20161 ctgtataaac ttgaccttct gcggctgaga caccaggctc aatacaaaac gccacttgtc
    20221 cattaatctc aaaacgaacc atataattta aagtgaagtt tagattgttc ggactagaag
    20281 taacaccaat attttcgccc caatcaattt tccgaccacc taaaacagct gcattggttg
    20341 cacggcgaac acgaaccgaa cgattggttg gtactgttac taacagtgta ggtgtacctg
    20401 tttttggaat gttctgctct tttttctttt gattttcaat actagccgta gattctttgt
    20461 ttggtttcgt tggttgattt tgactagctg cggttgaaga gattgttttt tcaacgtttt
    20521 cctcattttt tccttctttg tcagcagtag ccgctggttg ttccactgtt tcttctattc
    20581 gtttcttttc ttccgcttgt ttctcagaag actctggctg cacagaaggc gttgcttcgg
    20641 cagctttttc tccttctaga gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt
    20701 cttcaactgg ttgtgaagta ccaggactat tccctgggtg ttgtgtttcc ccaatcgtct
    20761 gatcgagagt cgttttggaa ttgaccgttg gcgcttctgt cacaggggcc tgttcaacag
    20821 tggcttcacc agctgggacc ccggcttctg ctggttggac ttctgtcact tcagaagcga
    20881 cttgggcaat ggcttccatc gtaggttgta tagccgtcgt ttcttctgca tgtgcttgag
    20941 cagttcccac caagaaacag aattgagaaa aagccaccaa tgcgagtgct ttcaaccaat
    21001 tcattttcgt ttttgcatac acaagaattc ctccttttcc tgagaatttt atataataaa
    21061 acaaaacgcc gttaccaaaa gtgataacga cgtgaactgt ctcttctatt tttatctggc
    21121 gctcttatct gaacacattt catcaatgat tttaccctcc cactataatt acagaacaaa
    21181 aaaagaacct gcttcgtgga aatcaactgt ccatttccca attggaatac cagctttttt
    21241 ggcaacttca ccatcaccaa atctcatgcc ataccaatta ctaccttcat ccttaatttc
    21301 tttccaacca tatgacatag cttcatctaa tgaatcaaaa atcatgacac tattgccaat
    21361 tgttacagga tctggcttaa gatcttctgg aagagaaata tcaatcatac cataatgatc
    21421 cgattcctca gtattattgt cattactagc tactggtttg gttgttctac tggcttattt
    21481 tgatacaaca agtactctta ttcttctttt acttctccgt tttcaatttc ccattcaaac
    21541 cactccgcaa agtcttgctt aaaaccatct actcgatacc tattttgtaa ataggcatct
    21601 tctgacgtcg taggatctaa ataggtaggt ttattctctg gacgagcata gacacctacc
    21661 catgtttcgt aatctccttc aaaataacta tctaccaaat agcctttttt tctaagaatc
    21721 tcccgaactt gatttttaat tattgatact tgttcaaaac catcactcat tctatcacct
    21781 caaataactc tagattctca taaatattcc cgacaatttc aatcctatca atgatctcta
    21841 acaaatcgct acttatgctt ccccattcgt ataagaattt cccttccgca aaataaattt
    21901 tccataaact tcttcatgat cgtcccaacc aatatcactc tcgaaaaatt ccacgttgtt
    21961 cttatctttt agctcagtaa atgccataag ttcaacatgt tttccaacct cgaaacaatc
    22021 acaccaccca tcaaccgtta cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt
    22081 attaaacact aaaactttcc gcattttttg ctcattaatt aatcacgcgc gaaatctagg
    22141 aatcacaact tattcgcttc ttctttataa
//
LOCUS       DAZKOU010000048        22003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000048 DAZKOU010000000
VERSION     DAZKOU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.048"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1432)
                     /locus_tag="ACG827_001702"
     CDS             complement(41..1432)
                     /locus_tag="ACG827_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0483845.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            1689..5078
                     /locus_tag="ACG827_001703"
     CDS             1689..5078
                     /locus_tag="ACG827_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0483846.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            5081..6502
                     /locus_tag="ACG827_001704"
     CDS             5081..6502
                     /locus_tag="ACG827_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0483847.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            6499..8376
                     /locus_tag="ACG827_001705"
     CDS             6499..8376
                     /locus_tag="ACG827_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0483848.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            8468..9310
                     /locus_tag="ACG827_001706"
     CDS             8468..9310
                     /locus_tag="ACG827_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0483849.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(9462..10337)
                     /locus_tag="ACG827_001707"
     CDS             complement(9462..10337)
                     /locus_tag="ACG827_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0483850.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            10544..11574
                     /locus_tag="ACG827_001708"
                     /pseudo
     CDS             10544..11574
                     /locus_tag="ACG827_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11663..11968
                     /locus_tag="ACG827_001709"
     CDS             11663..11968
                     /locus_tag="ACG827_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0483851.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            12147..14162
                     /locus_tag="ACG827_001710"
     CDS             12147..14162
                     /locus_tag="ACG827_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0483852.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            14164..14448
                     /locus_tag="ACG827_001711"
     CDS             14164..14448
                     /locus_tag="ACG827_001711"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0483853.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            14474..15847
                     /locus_tag="ACG827_001712"
     CDS             14474..15847
                     /locus_tag="ACG827_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0483854.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15930..17945)
                     /locus_tag="ACG827_001713"
     CDS             complement(15930..17945)
                     /locus_tag="ACG827_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0483855.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17967..19181)
                     /locus_tag="ACG827_001714"
     CDS             complement(17967..19181)
                     /locus_tag="ACG827_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0483856.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            19401..20345
                     /locus_tag="ACG827_001715"
     CDS             19401..20345
                     /locus_tag="ACG827_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0483857.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            20641..21585
                     /locus_tag="ACG827_001716"
     CDS             20641..21585
                     /locus_tag="ACG827_001716"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0483858.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7490 a         4011 c         4144 g         6358 t
ORIGIN      
        1 ttttatcagg gtggtttata catagctgtt ttttgattat tcataactcg aataagcgga
       61 tgagttcttc ttcatttgcg aatgacacac tatcatcaat caatatatga ggaacatggg
      121 cattcaaaat gggttgattc gtaacgatca gatcatactc gagaagattg tatttcgaca
      181 gctgatcttc agtaatagca gttgtctcag agtgaatttt atgcacagca agaatatcac
      241 tgatatattc ttcaatatat tgactatgtc gaagacttaa gtcgctgata atcagtattt
      301 ttttatcaga agtataatgg tgaatttcag gattaccgat aagcatccaa aagatatagc
      361 tagataaata ataaatcaga ttctgattca gtagagtgga ataaaattga agttctgcac
      421 gaaatccagc atatagacgt atctggcggt gttgcatctg ctgtgcaaag taagttgcac
      481 ggatcgtcaa atgagtcgta tcgtatggga aaatacggga gaaaaaataa atttgcgtta
      541 ataattgaac aacatattct tcgttttttt ctttgcaatc agcggatgaa aacgaacagt
      601 aggacatatt ttttcgtatc tgctcactga tttcattttt ttctttctcc gaaaattctg
      661 aaaaaatcgt attgacaaaa tagttcacta cttttatata gctgctgtac ttcaattttc
      721 ctaagagatt tttcaagatt aagaagtctt ttttatcgat atttatcaac tgtacataag
      781 agtctccagc tttccattca aatggagttc cttgggattc tctcatgaga tgcacaacaa
      841 aatacataat aaagaaagat tgctcatatg ggctttcttc ctgaaacaaa ttcatctgac
      901 aattattctt gaccataatt tctttgatct tctttttttc gattggaaat ggcaacgtgc
      961 gtcttccgta gtaacttaaa aagaaaaagg taacaaagat acgcaactgg agctcacttt
     1021 taaggctagt taactgaaaa ccttggtcaa cgatcacttt taataaatag ctttctaaaa
     1081 tttgattgca ttgtttcaac ttacgtgtaa aagaagaccg gctgatggaa attttttcac
     1141 aataatactc aatcgtttta cccggattga caataatctc atttaataaa cgactgtttg
     1201 tagaactttg cgacatttcc atcagaacat ttaaataagc tggtacattc aaatgttcaa
     1261 gacgataccc caatttttgt gaagattgaa tcgttacatc cgttccccag cggattttga
     1321 tctcttcgat ataccttaca aatgtagatt ctgaactacc aattcgtttg gcaagttctt
     1381 ttttagtcat ccacttgtca gaaatggaca gaattcgaat gattttttcc atctttctat
     1441 agtttaattc caacaacctg atcatatgtt tttctccctc aacccttttc tttcaatgaa
     1501 acgctcatta atcgaaggaa tcaacttcat aaatgaaacc aattggtatg aaaattggtg
     1561 tgattccctt tagcgccctt cgtataataa ttatcatatt aaaataaata aaattatgta
     1621 acgcctgttt attttaataa ttttcactat tcacgtcaaa acagaaaaaa agaaaaagga
     1681 ggggaaaaat gacgaccaca gggaagaaac tgaaagttat tttcatgctg ataatattga
     1741 gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac tacagatgat ccaacagttt
     1801 tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg aaaaaaagca ctgaacatca
     1861 agctaaatgc gaataacacc agtgctgaaa agatagaaaa agaaattggt ctagtcgaaa
     1921 attacttaag tgatgtggaa agaaaagaag gagatggcta tgcttatcag gtaaatagcg
     1981 ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac tatcgatctg agttttccaa
     2041 tcgatccagc actttaccac agccaggcaa acaagctgat cgtcgataat aaagaatatg
     2101 acattattga tgagacagaa aataagaaag atacagatgt gtcagtacca aagccagacg
     2161 aaatagaaga agaatcatca aaagaaaacg aaaattctgt cagcccattt acattgccta
     2221 cattatcctt gccagctgtg agtgtgccat ctaatcaaac gattcctaca gaatatacaa
     2281 cagatgatca gggcacttat cctaaagcca gttggcaacc tacaggaaat acaaatgttc
     2341 ttgatcatca aggcaataaa aacggaacaa atcaatggga tggtataaat tcttggaatg
     2401 gagatcctaa tgatcggacc cattcgtata tcgaatatgg aggaaccggt aatcaagcag
     2461 actatgcgat acgaaagtat gcaaaggaaa caagtacacc cggattgttt gatgtttatt
     2521 tgaatgctcg tggaaatgta caaaaagata tcacgcctct tgatctcgta ttggtcgtag
     2581 actggtcagg aagtatgaac gacaataatc ggatcggtga agtaaagatt ggtgtcgatc
     2641 gttttgtcga tactttagca gatagcggta tcacagacaa aatcaatatg ggatatgtcg
     2701 gctactcaag cgaaggatat agctacagta acggtgcagt acagatgggt tcatttgatt
     2761 cagtgaaaaa tcaagtaaaa tccattacac cttcacggac aaatggtggt acttttacac
     2821 aaaaagcact aagagatgca ggaagcatgc tatccgttcc aaatggacat aaaaaagtga
     2881 tcgttttgct gacggatggt gtaccaacat tttcctataa agtacagcgg gtacacgcac
     2941 aatcaagcag caattattac ggaactcagt tttctaatac gcaagatcgg ccgggaaata
     3001 cttctctaat ctcaagaatc tatgatgcac ctgaccaaaa caatctatcc agaagaatcg
     3061 acagtacgtt tatcgcaacc atcggagaag cgatggcact caaagaacga ggaatcgaaa
     3121 tacatggtct tggcatccaa cttcaaagcg atccggcagc tggtctctca aaagcagaag
     3181 tagagtctcg tatgcgacaa atggtttcat cagatgaaaa aggcgatctt tactatgaat
     3241 cagctgatca tgcaacagat atctctgaat acctagccaa aaaagctgta cagatctcag
     3301 caactgtaag caatggacaa ataaatgatc caatcgcaga accattcatt tatcagcctg
     3361 gtacactttc agtcaagagt gtggggacaa gtcctacaac ggtcactcca tctatttcca
     3421 tagaaggaaa taccatcaag agcaatcaga tctatttagg aaaagaccaa gaaatccaaa
     3481 tccattacca agtgagaatc caaacagaaa atgaggactt ccatccaaat ttctggtatc
     3541 aaatgaacgg caggacaact ttccagccaa acattgatac caatgaatta gctgaattcg
     3601 gtataccatc tgctaaagct cccggagtca gtcttcacat caaaaagtta tgggaagaat
     3661 ttgacaacaa tctagctgat cgtccagatc aagttacttt tgagattcaa cgggaacata
     3721 cgacaaatgc tgcagcttgg aaaaacggat atattcgaat cattaaacca gctaaagata
     3781 caacaaatac gtgggaacgt gcagacattg acaaattatc tgcaaatagc ggagaaagtt
     3841 atcaagagat attatcacta cctcaataca ataatcaagg tcaagcattc agttaccaaa
     3901 caatcaaaga attacctgta ccaggatacg attctcaaca aatagatgca atgacatgga
     3961 aaaatactaa acaattcaca ccgttaaact tgaaaataac gaaaaattcc tctacaggtg
     4021 aaaaggatct tattggcgct gttttcaaat taacaggaga ttctattgat actttactaa
     4081 cagatcatgg cgacggaacc tattctcttc cagaaaatgt caaattgcaa aaagaaatga
     4141 cctatacgct gacagaaaca aaagctccag aagggcatgg attaagcaaa aagactactt
     4201 gggaaatcaa gatcgcttct gatggtacgg taaccattga tggaaaaaca gtcactactt
     4261 ccgatgatac gatccagttg actattgaaa atccttttgt tgaagttcct gtagcagtac
     4321 gtaagtatgc gatgcaaggg acggacaaag agataaatct taaaggagca gcattttccc
     4381 tacagaaaaa agaagcaaat ggtacttatc agccaattga cagccaaaca acgaatgaaa
     4441 aaggtcttgc cagttttgat tcactcacac ctggtaaata tcgagtcgtt gaaacagctg
     4501 gtcctgccgg atatgatact tcgccgggaa attatgaatt ccaaatcgat aaatatggaa
     4561 aaatcattta cacgggaaaa aataccgaga tgacaaataa tgtatggacg ctcactcatc
     4621 aaaatcgact aaaagcgttt gatctaacgg tacacaaaaa agaagacaac ggacagacat
     4681 taaaaggagc aaaattcaga ctgcagggac cagaaatgga cttagaatcg ccaaaagatg
     4741 gacaagaaac agataccttt ctattcgaaa atttaaaacc tggaacttat acgctgaccg
     4801 aaacttttac accagaagga taccaaggtc taaaagagcc agttactata gttatacacg
     4861 aagatgggtc aattcaagtg gatggacaag atcatgaatc tgttctgtca ccaggagcca
     4921 aaaacaacca gatttcttta gacatcacga atcaggcaaa agtaccatta cctgaaacgg
     4981 gaggaattgg ccgtttagga atctatctag tagggatgat tggttgtgcg ttttctattt
     5041 ggtatctttt tttgaaaaaa gaaagagggg gcagctaaat atgaaaaaac ttggttggct
     5101 tagtatgtgt ctcttcttgt tactatttaa accagctttt actcaggtag caacagaaac
     5161 agaaacagaa atggttcaga ttactttaca caaattgctt ttcccaaacg ggcaactgcc
     5221 gaaaaatcat ccaaatgacg gacaagaaaa agctttatta caaacgtatc gaggattaaa
     5281 tggtgtcaca ttccaagttt atgatgtcac agattctttt taccatctac gggaaaaggg
     5341 caaaacggta gaagaagcac aagcagagat cgcaaaaaac ggtgcgtctt ccggtatgtt
     5401 taccgcagaa gcaacaacta caactcttaa caacgaagat ggtatcgctt ctttttctct
     5461 ggccgctaaa gatcaagaaa aaagagataa agcgtatctt ttcattgaat ccaaagtacc
     5521 agaagtcgtc aaagaaaagg cagagaatat ggtagttgtt cttcctgtac atggacaaaa
     5581 caatcaaaaa ctttcaacta tccatttgta tcctaaaaat gaagaaaacg actaccctga
     5641 tccacctttt gagaaggtat tagaagagcc tagaaatgat tttacgattg gtgaaaaaat
     5701 cacttattcc ttgcatacga caattcctgt aaatatcctt gactatcaaa agttcgaatt
     5761 gtcagatagt gcggatgaag cattaacgtt tttacctaat agtttaacga tttcatcgaa
     5821 tggagaaaag ctgacagaag gctttgtcat acacaagaaa cctcacggat ttgatgtttt
     5881 attttcgatc ccttcgttgg aaaaatatgc tggaaaaaaa ctgaccattt cttatcagat
     5941 gcagctaagc agtacagcac aggcgaacaa ggaaatcaac aacaacggaa cactggattt
     6001 tggttttggt gtcagtacaa agaaagtctc tgtatataca gggagtaagc aatttgtcaa
     6061 aatcgagaca aataaaccag ataaacgatt agctggcgca gtattcctta ttaaaaacaa
     6121 agcaggaaat tacctccagc aaacagccaa cggatacaag tggacaaaga acgaatcaga
     6181 tgcgcttcac ctgatttccg ataaaaatgg cgctttttca atttccgggt tgaaaacagg
     6241 aagttatcga ttaaaagaga tcgaagcacc ttctggttat attttaagtg aaacagaaat
     6301 tccgtttacc atttcaactt ttctttctga ggataaagag gcggacagta tattgaaagt
     6361 agtcaataaa aaagaaaata gccgtccatt tcttccaaaa acaaacgaaa cgaaaaatac
     6421 acttttaggc gttgttggta tggtattcgc aagctttgca atctggttgt ttatcaaaaa
     6481 aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa acgttatgtt aggagtcctt
     6541 ttccttattt taccattact cacaaacagc ttcggcgcaa aaaaagtgtt tgcagaggag
     6601 acagcagctc aagtcatcct tcataaaaag aaaatgactg atttacccga tcctttaatc
     6661 caaaacagcg ggaaagaaat gagcgaattc gatcaatacc aaggattagc cgatatttca
     6721 ttttcagttt ataacgtcac tcaagaattt tatgcgcaac gagataaagg agcgtccgtg
     6781 gatgcagcaa aacaagcagt ccagtctttg actcctggta caccagttgc ttcaggaacg
     6841 acagatgctg atggaaatgt cactttatct ttacctaaaa aacaaaatgg gaaagatgca
     6901 gtctacacga tcaaagaaga accaaaagac ggagtgtcag ctgccgcaaa catggtttta
     6961 gctttccctg tatatgagat gatcaaacaa gcagatggct cttataaata cgggacagaa
     7021 gaactagata ctatccatct ctaccctaaa aatacagtcg gtaatgatgg aacgttgaaa
     7081 gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg gagcagaatt tattatttct
     7141 aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa gtgtcacaga tggattgtac
     7201 acttggacaa ctgatcaaac caaagccaaa catttcatta ctggtcattc ttatgacatc
     7261 ggcaacaatg actttgccga ggcatctatt gaaaaaggcc agttgatcgt taatcattta
     7321 gaagttggaa aatataattt agaagaagta aaagctcctg ataatgcgga aatgattgaa
     7381 aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc aaacaccagt agaaaagacc
     7441 atcaaaaatg atacgtctaa agttgataaa acaacacctc aattgaatgg aaaagatgtc
     7501 gcaatcggtg aaaaaattca atatgagatt tctgtcaata tcccattagg tatcgctgat
     7561 aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta tcgatactca tgacgctgct
     7621 ttaacatttg ataatgattc ttcaggaacg tatgcttatg ccttatatga tggaaataaa
     7681 gaaatcgacc cagtaaatta ttctgtcact gagcaaacag acggattcac ggtttcagtt
     7741 gatccgaatt atattccttc attaactcct ggcggtacat tgaaattcgt ttactatatg
     7801 catttgaacg aaaaagcaga tccaaccaaa ggattttcta accaagcaaa tgtcgataac
     7861 gggcatacaa atgatcaaac accaccgtca gtcgatgtcg ttactggggg caaacgattt
     7921 gttaaagtag atggtgacgt tacatcagac caaacacttg ctggagcaga attcgtcgtt
     7981 cgtgatcaag atagtgacac agcgaaatat ttatcgatcg acccatccac aaaagccgtc
     8041 agctgggtat cggcgaaaga atcagcaacg gtttttacaa ccacaagtaa cggtttaatc
     8101 gatgtgacag gtctaaaata tggcacgtac tatctggaag aaacgaaagc gccagaaaaa
     8161 tatgttccat taacaaaccg tgtagcattt actatcgatg aacaatctta tgtaacagca
     8221 ggacagttga tttctcctga aaaaatacca aataaacaca aaggtacact tccttcaaca
     8281 ggcggtaagg gaatctatgt gtatatcggt gcaggagtag tccttctact gattgctgga
     8341 ctgtactttg ctagacgcaa gcacagtcag atttagtctt tttgcaagaa ttggaaaaag
     8401 gagcagcgga ttacgcaaat gctccttttt cttttattat caaacagtca tcacaaggag
     8461 aacacaaatg aaaccaaaga aaaggaaact tctagatctg ctgatgcttt tcttgcttct
     8521 ctcaggaata gggatactag cttatccatt tgttagcgac gcgctgaata attatctgga
     8581 tcagcagatc atctctcatt atcagcaaca agcggtcaaa gaaaatgaag aagtcatggc
     8641 taaaatccaa gaaaacatga caaagaaaaa ccagcaatta gcaaaagaag gtggaaatcc
     8701 aggtgcagat ccctttacta agaaaaaaga acctgcgaaa aaggatagaa cctattttga
     8761 gaaacatacg atcggtattt tgacgatccc tgagatcaat gtccatttgc caatttttga
     8821 tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta ttggaaggaa cgtcttatcc
     8881 tacgggggga gaaaatacac atgccgttat ttccagccat cgaggactgc ctcaagcaaa
     8941 gttgttcaca gacttaccga aactgacttt aggtgattct ttctatatcg aaatcaatgg
     9001 acggacactc gcttatcaag tcgatcagat caagacggta gaaccgactg atacaaaaga
     9061 tctgagaatc gaaaaagggc aggatttcgt gacactccta acttgtacac cttatatggt
     9121 caacagtcat cgcttacttg tcagagggca ccgggttcct tatcatgcaa aagaagttca
     9181 aaaagaagta cagaaagtat ccgagaacaa acgatttttc ctgtatggat tgttagctgc
     9241 atgtatactg attttcctcc ttcttatata tctttttcgt caatcgttca aaataagaaa
     9301 cagaagttag ctattcctca tttctataat atatctattc gaatcgttcc gtaaacaaac
     9361 aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat gatgatatca aagagagcct
     9421 atcaaacact atctttctta ttactaaatg aggatctttt cttaagtttt ccgatattct
     9481 ttaagaaggc ctttttgcat caattcttgt ttgtttttca agttgcggat attctcttca
     9541 aaattttgag aatatatcac tgagaataac gtatttaata cgtaatcgaa ggcaatttga
     9601 gaggagaaag tcgctacttt agcaaaatca tattcctcct gcgatataac aagacacata
     9661 tcagcctgtt tggccatttc tgaatgctga tttccggtaa tcagcaaggt aggggcctct
     9721 acatgcttca aataggtcat tattcgttca taatgatgga tgcgaccgct gtagctaata
     9781 aaaatggcgc aatcctcgct agtcaaattg gctgcattcc aagaagaatc actgtattct
     9841 tcagccatga tcaaaaactt attcaacttg accattttat tctggaattt ccttgccgtg
     9901 atttgagaat caccttttgc aaaaagaaag attcgctgtg ccttgttcaa tactttaccg
     9961 atttgatcta aatcttcatg ttcaatctgc aattgtgctt tttttattga ctggatcgat
    10021 aaatccgcca ttttctttgc aatttgctga gtgctatctt cacttgaaaa aggaaaatta
    10081 ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa cttcccgccc cagctcgaaa
    10141 cggaattctt taaatccgct gaatcccatt tttttgcata aacgaatgat agctgaatgg
    10201 gaagtatacg tagcttttgc taattcttca atcgtcatgt agactgcagc ttcgatattc
    10261 ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa acaactcttg ttttgttagt
    10321 ttttcttcaa taagcatgat ctgacctcca aattctatct tactgataaa cacttggttc
    10381 cgctactatc tttttgattt ttatccttac tttgaacatt aatttcacca aaccgaatga
    10441 taacaagtgt ttttctgaac ttttttacca gaatagctat tataaatact ttctgcgctt
    10501 ttctaggata tactgaaaac atcaataaaa ggaggcacca ctcatgctta caatcgctta
    10561 tcttggcaat ggaaagagta caaatcgcta tcatttgcct ttttcaagaa aacttacgaa
    10621 caaaatcaat atcaaaaaaa ttttttcccc atctgaacca gtgtggaaaa cattcgaaga
    10681 catcgaatat actgatcagc tgtctgatct ttgggatgat cctgagatcc aattagttgt
    10741 catcactaca cctagtcaat ttcattatga atatggaaaa atggcactcg aacatggtaa
    10801 gaatgtacta gtagagaaac cttttgcaga aacgtcaaag gaagcagaag agttatttgc
    10861 attagcaaaa gaaaaaggtc tatttatcca atgttaccaa aacagacggt tcgattctga
    10921 cttcttgaca gtccaaaaag taatcgaaag cggaaaatta ggggatattt tagaagtaga
    10981 aatgcattat gattatttca gaccggaagt accagaaaat acgcatgaat tttctactgc
    11041 gaacagctat ttatatggac atgcttgtca tacagtagat caagttcttt cttatttcgg
    11101 cgaaccagat gatatcagct atgatgtacg tcaactttta gggaaaggac gaatgaatga
    11161 ttacttcgat ctggattttt tctatggaaa tatgaaaatt tcgataaaat ccagttattt
    11221 ccgcatcaaa gaacgtccaa gtttcgtcgt ttacggaaaa aagggatgtt cgtgaaacaa
    11281 acaaaagatc gacaagaaga acatctgaaa atgttttaca tgcctgatca tgaagacttt
    11341 ggtatcgatt tgcccgaaca ttatggtgta ttgacctata tagatgaaca agggagttat
    11401 catgaagaaa aagtaatttc cgaggttggt gattacagcc gtgtctacga agggatttac
    11461 gagacattag ttaatggcgc agaaaaagtc atcaaagatg aagaaacaat cttacaaatg
    11521 aaaattttgg aagaaggaat caaaagcatt caagaatcag aaggaaaaat atgacatatt
    11581 tgtaagaatc gggtttgttt tttgacgcat aaactgtctt attgtacgat gaaaaagtat
    11641 agaactacta ggaggaacca acatgacaaa ttatgacggt gaaaaacaca aacgattaag
    11701 aggttcaatg gcccctaaaa cagaagaaga agtggcttta gcaggaacta attctggtga
    11761 aacagaagac aaagaagaat cattagctgg cacaaactct ggtgaaacag aaaataaaga
    11821 aacagcttta tctggttcaa attctgtaga agaggaaaca aagcctaatg agcccaatca
    11881 ctttgaaaaa gatatcgaaa aagcggttga agatgtcaaa gaaaaatttg aagaactgac
    11941 ctcaaaaaat aaacatgatc atcattgatc ggacgaaatc aattcataaa agcaggagac
    12001 atttggtcac ctgctttttt tctgtatcca ttcgatagaa atcgttcggt acagtcgtaa
    12061 aaaaaatcca actgtaagtt gtaccaaaca cccctcgatt taaaagcgct accatactag
    12121 aatataagta ggaggtgaac agcgctgtga actacaaaat tagttatctc ctgaaaacac
    12181 caagagaaat cagtctagaa aaagcagcag agatcatcga agtaaataca agcgaggcga
    12241 tcaaacaatt aactcctttt gccgaatatg tggatgatac ccgtatccgt ataaagaaat
    12301 tccgagcaat cgactgggca gaaaaatttt tggatattac tcgatttgaa gctgtgtata
    12361 ccgaacaaga gcgaagagat cttctttatt tgatggttta ttcgactgca acgtctgttt
    12421 ctgtttttca ttttcaagat tttcttcagg tttctaaagg gacagtcctg tcagatatca
    12481 aaaagataag aaatgaatta gaagtggaag attgtcagct gtcgtatact aggaaaactg
    12541 gttttaggct gctagggtca gaagcaatat taagaagaga agcaaaaaat cgcactgctc
    12601 gtttgatgca gtcacttgct ggaagatttg ggctcacgta tttaaccgca accctttcca
    12661 ttgattatta cgccaaagtt agagatttgc tagataaaac gatttcacta tcagatcttc
    12721 gattcgcacc aaatcgaatg gatgaattag cttattattt gagtttgaca accattcgaa
    12781 ttggaaaaac atccatggaa ctggtaaaag atgctgaact gttggcttct cttgcagtca
    12841 ctgacattag tcgacaagta ttgactggcc ttggctattc cgaacatcaa aattcggaga
    12901 ttatattttt ctctctttgc ttagcatcga tcgttgaagg agatgtccag gagcctgcac
    12961 ttgatttttt gctggaatgt tcttcagaag tcatccatcg catggaattc ctgatggccg
    13021 tagaatttgc ttcgtttcgt gaattattga tgaatgtctt ttatcacttg gtccctgcct
    13081 attttcgtat ttcctattct ttttatttac ctaatgtgat gatcgatcaa attaagcatc
    13141 aatatgcttc catctacgaa atgacaagaa aagcattact tccgttagaa agacgaatag
    13201 ggaagagcat ccctgaagaa gagattggtt ttttcactat actatttggc ggagagatca
    13261 gaaaagcgga cgcagaagaa agaaatagaa aaataagagc cgtgatcgtc tgtccaagtg
    13321 gcattagctc ttcattgatc ttaaaaagtg aacttcagca attattcccg atgatctttt
    13381 ttacagagac taattcagct gaccgcttac acgaggttga cgaaacttct tacgatatta
    13441 ttttttctac tgtacctctc acacttactg ataagaaaaa gtctctctat gttctgcatc
    13501 caatcatgac tgacttggaa aaaaatcagc tgatcaatca agtacaaaga gattggctga
    13561 taccaggatt ttcaatgcct tctgcccaag aattgttgtc ggcgctactg ccttatatcg
    13621 aattaaaaga aggcgtagac gaagaaaagc tctatcaagt attaaatcgt aaaatgaaca
    13681 aacttttaga aaagaaggag gatcaacgtc ctatgctatc tgaattatta acagaagaaa
    13741 tgatccaatt atcggatcaa ccaaaaaact ggcaagaagc aatcacattt gccgcacagc
    13801 cgctattatc agaaaataaa atagaagaaa gttatatcaa agctatgatc gagcgcgtgg
    13861 agcagtatgg tgcgtttatc catattggcg atcacatcgc attacctcat gctcgtccag
    13921 aagatggcgt aaacgaagta gggatgtctt tgctgaaact tcaacaacct gtggatctgc
    13981 ttgataatcc agcccatccc attagtttgt tcatctgttt agctgctatc gataatgagg
    14041 cacatcttcg ggctttggca aatttaacaa agctactttc taataaacaa aatcttcaag
    14101 atttattaca cgcatcaaca aaagaagaaa tattagcgat cataagaaaa ggagacgaat
    14161 gatatgaaaa tttttgcagt atgtcaaagt ggattaggaa caagttttat ggtagagatg
    14221 aacatcaaac aagcacttgc agatcttgga gcagatgtgg acgagtttga agtaagtcat
    14281 acggatgttg gaagtgtatc tagcgatatg gctgattatt tcttcattga gcattctttg
    14341 aaagatacgc taacttctat acctgatgaa aagctcgtac ctttgcaatc gatcattgac
    14401 agcgaagaag taaaagaaaa agtaacgaag atcttgtcaa aagaataaaa atgattcaag
    14461 ggaggaacta tcaatggata aattgttgaa tttgtttgtg tcaatcgtca gtcagccctc
    14521 catcctcgtt tctttgattg cactgcttgg tctcgttctt cagaagaaga aattttcaga
    14581 agtgatccaa ggaactatca aaacatttgt cggattccta gtactgatcg gcggtgctgg
    14641 tttaatcagt aattcactaa ccccttttgc ttcgatgttc caaacagctc tgcatactca
    14701 aggagtagtt cctagcaatg aagcagtagt ggcgattgct ttgcaacaat acggtacacc
    14761 tactgctttg atcatgctag tagggatgat cgtgaatatt ttacttgcta gattcacaag
    14821 atataaattc atttttctta caggtcaagc aatgctttat gtttcttgtc taacagcagt
    14881 cattttagta agcgcggggt ttactgcaaa tttgtggatg atcttattag gcggggtctt
    14941 cgaaggaaca ctattaacta ttacgcctgc tctttgtcaa ccgtttatga agaacatcac
    15001 tggaaacaat caagttgcgt tagggcatac cggaaatatc ggttatgctt ctagtggatg
    15061 gatcggaaag ttttttggaa acaaagaccg ttctacagag gacataaaga ttcctaaaag
    15121 tttcagcttt ttgcgagatt ctactgtatc gatcatgctc ttgatgtcga tcgtatatgt
    15181 cgcacttgct ttgttagctg gtcctggatt tgttgaaaga gaactaagca atggagacaa
    15241 cgctgtaatc tttgcactga tccaagctgg aacattcaca gctgggtttg ttatcgtcct
    15301 tcaaggggtt cgaatggtgt tggcagaaat cgttccagca ttccaaggga ttgctaaaaa
    15361 actcgtaccc gattctaaac cagcattaga tgttcctatc gtttttccat atgcacctaa
    15421 tgctgtatta attggctttt ttgttagctt ctttgtagga acactatcca tgtttgctat
    15481 gataggcttg caaactacag ttatcattcc tggggtagta ggacatttct tctgcggagc
    15541 tgcttctggt gtcttcggca actcgactgg cggtagaaga ggggcggtat taggttctgc
    15601 tttcaatagc ttattgatta gttggctgcc attatttatc cttccagtat taggagatct
    15661 taaaatcgct tcttctacat ttgccgatac tgattatcta gtaccaggac ttgtacttgg
    15721 gaaactagga gaatacggtc aaatagcctt gacagttgga atcatcgctt tctttttact
    15781 tgtcgtatta tctagctttc tttttgccgg aaaaagaaca aacattgttt ctaaagaaca
    15841 gaattaagca aacttcaaaa taaaaattgc ttggacaata tcagaagaag atttaaagtc
    15901 ttctgatatg tcgcaagcag ttttttgttt tatgcattta tttttgaata gcttgttaag
    15961 acaagtggct gttttgctag aagaggataa cttttaccat tcattttcac tgttccgcct
    16021 gttctatcag aagtaatcac gatcttatct tttgtttttt caagcgaaat ctttccatct
    16081 ttgataggta gcgtacaatt cagtgtttca aatgaatcta gattaggttg tatcataaat
    16141 tcttcataac ctggctttgt tggatacaaa ccgataaaat agcgtcctaa aagatagatt
    16201 gggcttgctc cccatgcatg gcataagctt tttccataag gatctccata catactgtac
    16261 ttttcatttc ctgtttgagt agggtcgtat tcttcccaga aagttaccgc atcatgagat
    16321 aacatccctc cccaataatc aagaatcgtt tggtagacga tattcgtctg tccgagtttg
    16381 cataaagcat cttgttcata gaatttgaaa tatggagtag taatctgtgt gatagtgtca
    16441 ttcaataaga cattctttaa gatcgattgc tgttggtctt tgttagcaaa atcgaataaa
    16501 acagcgaaaa tattcgcatg acgcgtcaca tttcttctcc cagattcata gcaatcgata
    16561 aaagcgccaa gttcttcatc ccaaaagtag tcaaataggt tttgtttcaa tagttgatat
    16621 ttttcttgat agagatgagt atgataattt tcgttctctc catatgtttc agctaagata
    16681 tctccgcaag tgatcatgga atgataggct ttcagtaata agacttgttc tgcagcaagc
    16741 gttccttctt tatccatttc agaccaatca ataaatatcc aatcttttgc tctcccataa
    16801 ataaacccta attcattcgt ttgctccatc aagtatctca ttaatgattg catcttaggg
    16861 tagacgatgc gtaaaaattc atagtctttc gtcatcatat agtggttttc gataccaatc
    16921 aaccacaaga tcgaatagtc aacgatagta ttcatatgct gcttgatttc attttgtcct
    16981 cttaatgcta atatcgttcg tttattgatt tcctcgtcaa aaaaaagata ttgattgatc
    17041 atgaatgatt gatacgcatc tccagaccaa atccagcgat ctcgtttgat accgtcgata
    17101 aaaaacaaat cactacataa cttgaaggct tcttctgata ctgaccagat ctgattcagt
    17161 aaagaagaat cgcttgaaaa agcagcttgt ttcgaaagag ataaaaattc atgaaaaact
    17221 tgaagttgaa ccattccagt agaaacctct ggtataaaca aatagcgaaa agcgcgttta
    17281 cgagctttca tttttcttgt cattcctatt tgcttatagt agcaataatc gatatccata
    17341 gcttctacat cggactctcc ataacataga gtgatagatt cgatatggtc atccagagat
    17401 attagttcta cacagccatt cactgctcgc ccaaaatcga ataagattcc tccgccaatc
    17461 tccgtttcag tgactggatg gacgcattct ttttgataat agatttgatt tggatctttt
    17521 gcgatatcga cataaagcgg actccagcca gctggatatt cttcaataaa attacttgct
    17581 gtccagccaa tatctgattt tacttcgtct ccctcgataa acatcgctgg tagccctgat
    17641 gtatgtccta caaagatacg aatttttgct ttaccagctg gacaagtcag ccattttcca
    17701 catggatgtt tttgtccatt aatttctaca tacccaactc cttggatcgt cactttaaac
    17761 atggaaggtt gatcaagaaa gtaatatctc ttaaacttga cgttccgatg gcaatcatcc
    17821 atgtaccagt acgctggcca gtcaaaacct cgttcttccc gttggaaatt ttgtagcagt
    17881 ccatggcgga tctcaaagtc tccaggatac caaagccatc tatctttatg tttttcattt
    17941 gtcattttac acgacctcca tcaaatttat tttgttgtca attgttcttt tatttcttca
    18001 tctactctta tcactgtctt tctattcgtc ctgtgtacta ctagaaggct aataagcgcg
    18061 ctaatcaaca gcaagatact gccattgacg aaaacagcaa gtgttttact cccaagagaa
    18121 gcaaaagaga gaaagagcaa accatatggt gcaatgaaag tacctaaatt agcgccaacc
    18181 aataagattg aagtattgaa actctccgcg tacttgggcg cttggatact tacttggttg
    18241 aataagtaag gaatgaataa cgaaaaagaa attcctgtta ggatcgcacc aaacccaatc
    18301 acatacaggt ttatcacagt ggcaatcaat acaaagccga ttcccatcag tagtaaagaa
    18361 agtgtaagca tatagcgatg tgcttttgtc tgaagccagc caaaacaaac tccggcagcc
    18421 atacttgcca attgcatgat tgttaagata ttcgctgcat tagtttcgtt tcctagagaa
    18481 taagtcgtca ggactgtggc taatttaatc gatacagcat tatataccac gatgaccagc
    18541 aagagaaaaa taccataact aatcatttta ggattcgttt tgattacttg tcttgttggg
    18601 gctgtgccat gcgattgaag ttcagttgtt tcaggtgtag gaacaaacaa aacaaacaag
    18661 acaagaatag gtaagctgat ggcgtagact aaaaaggtat attgccaacc gaactgtact
    18721 agctgtcctg caacaaatgt cattaaagct gcaccaaatc cttgaaatgc actttgaaaa
    18781 ccaatcatag aggctcgttc atctccgtga taaaaggcag caatcatgct gactgccaaa
    18841 gaattgatca atccaaagcc aatacctagt ccagctcttg aagctaagac aatactgtaa
    18901 ctttgtgaga agagggggag gataccacaa atcgatgcaa tcactaatcc gatgatcaca
    18961 gtttgttttt gtccgatttt cctagcaata aaactactaa tcaaaacaaa aatagtgacc
    19021 ataaaagagg gagcagtcgt taataattca acagcagata aagaacgcgt ggcataagta
    19081 gctgacatac tgggaattgc agacgtgact gcaccagcag aagtcaaaat aagagagact
    19141 gacagcagag aaaatttcat catcttttta ttcataagca tgttcctttc atttttgtaa
    19201 gcgcttatca aaaatatagt atgaagtggt tattctctca atagcggaat cacaggaagt
    19261 tcatgtccca accatagaaa gtctttatga aaccaaaaat agctttgcat ataatagcag
    19321 tacagcgtga actaataagc gaaagaaacc gcttacaaaa aggagggagt caattggaac
    19381 tagctgaatt agacattttt ttgcgtaacg aagaatcaat cgaaactctg cagcgtgaac
    19441 aaggaatgaa tatcaatgat ttaaacttag cttaccatct tcagacaccg ccaaaaatcc
    19501 accgagggga tctatttcaa cagggaaata ttcatatcag taaacaccgt cgatatgcgg
    19561 aagttcccga gcatacacat gattttgttg aaatcaatta tatgtatagc ggtacatgtt
    19621 tacaaattat taatggagaa aggatagaga tggaagaaag tgacgtggtg atcattgata
    19681 aagaagctac tcaaagaatc gaatacacag ggcaagagga tattttaatc aacatcttat
    19741 taaaagaaga aaccttatct acagagatat tgaatcattt ggcaagcaca aataatctac
    19801 tttcttcttt tttgataaat gcatcaaaag aaaaagggga acatacacaa ttcattcatt
    19861 ttcgcggcgg aaaaacaagt cgtttgacgc atttgattga aaatatgctg ttgatttatt
    19921 ttgatacata cagtcataag atccgttcgt taaatctcta tacatcacta ttactgatcg
    19981 aattgactca tttattagaa caacaaagct tttcatcagc agatggatgc gcagaaaatg
    20041 agatgcttag agtattagat tatatcgata agcattttac ttcttgtacg ttaaaagaaa
    20101 cagcagctca ttttggctac aatccggtct atcttagtaa taaattgaaa atgtattttg
    20161 atgcttcctt tcaagaactg gttctaaaga aaaaattcga ctttgcgttg gaactgattc
    20221 aagaaacgag tttttcgttg gaagaaatcg ctaatcgtat cggatataaa caaacgactt
    20281 ctttattcaa attattcaag aagtattcaa catgtactcc caatgaatgg agaaataaac
    20341 attaagaaga aaatctttca ctaaagcata tgaaggaaaa ttgttttagg tgcgattgct
    20401 gtcttattat atcgttgcag cgaatttttt tggatttatt ttcagttcta aacttttggt
    20461 tgtattatca ggggatatag gtgtaaacat ttgtcattta tctgttatgc gtattcagga
    20521 attcgtttcc atcaaaaatt ataataacaa ctattataat taacatgttg ataaaatgaa
    20581 taaacaagac tatacttttc atgaaagtaa caaatagtta tgaaaaggag ctgtttattc
    20641 atgaaaaaaa caagtcgtaa agtagtaatc gtcggtacag gttttgttgg aacaagtatc
    20701 gcttatgcaa tgatcaacca aggaatctct aatgaattag tattgatcga tgtgaatcaa
    20761 gaaaaagcag aaggggaagc attggacctt ttagatggta tggcttgggg cgatgaaaat
    20821 gtggctgtat ggtcaggtgg ctatgaagaa tgtaaagatg ctgatatcgt tgttatcaca
    20881 gctggtatca accaaaaacc tggtcaatct cgtttagatt tagttaaaac aaatgcgtca
    20941 atcatgcgcc aaatcgtaaa agaaatcatg ggatcaggat tcgacggtat tatcgttgtt
    21001 gcttcaaacc cagtagatat tttgacatat atcgcttgga acgaatctgg tctgccaaca
    21061 tcacgtgtta tcggtactgg aacaacattg gatacaactc gtttccgtaa agaaatcgca
    21121 ttgaaattaa aagttgaccc acgcagcgtc cacggctata tcttaggtga acatggtgat
    21181 tcagaagttg ctgcatggtc acatacaact gtcggtggta aaccagtgtt tgaaattgta
    21241 gaaaaagacc atcgtattgc ccaagatgaa ttagatgtga ttgctgataa agtccgtaat
    21301 gctgcttatg agatcattga tcgtaaaaaa gcaacttact atggtattgg tatgagtact
    21361 gcacgtatcg taaaagctat cttgaataac gaacaagctg tattgcctgt atctgcttat
    21421 ttaactggtg aatacgacga aaaagatatc tttacaggtg tgccatcaat cgttgacgaa
    21481 aatggtgttc gtgaagttgt tgaattatca atcaacgaag aagaaaaagc aatgttcagc
    21541 aaatcaacaa gtgctttaag agaagtattg aacactgtat tataagaaac aatgaatatg
    21601 aactaaaaat cctaaaactg cttaacagct gaaatagctg ttaagcagtt ttttattgat
    21661 atctaatagg taagcaaata aatagaatgg ttaaaattaa tcggtccgaa aatgtatccg
    21721 tacatatgaa catatgaaat ttaatctgtt gattacaata agcgaatcgt tatcaatgtt
    21781 tatctgtggt taattaaaaa atccttccgc aacggcaaat acaatgattc atatctaatt
    21841 cgttcatatt tgattaactg aataaagaaa ctggttttta tcattcaatt aaacgacaaa
    21901 aaaagtaaga aaaactgcaa aattttttta taattttgca taagcagatt ttgatggaaa
    21961 aatgacgatt tttgataaaa aaccgttcga gagaggttta tca
//
LOCUS       DAZKOU010000049        21924 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000049 DAZKOU010000000
VERSION     DAZKOU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.049"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..522
                     /locus_tag="ACG827_001717"
     CDS             283..522
                     /locus_tag="ACG827_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483859.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="ACG827_001718"
     CDS             complement(884..1144)
                     /locus_tag="ACG827_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483860.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="ACG827_001719"
     CDS             complement(1329..1826)
                     /locus_tag="ACG827_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0483861.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ACG827_001720"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ACG827_001720"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0483862.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ACG827_001721"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ACG827_001721"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0483863.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5210)
                     /locus_tag="ACG827_001722"
     CDS             complement(4548..5210)
                     /locus_tag="ACG827_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0483864.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5220..6035)
                     /locus_tag="ACG827_001723"
     CDS             complement(5220..6035)
                     /locus_tag="ACG827_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0483865.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6297..6740)
                     /locus_tag="ACG827_001724"
     CDS             complement(6297..6740)
                     /locus_tag="ACG827_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0483866.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6874..7212)
                     /locus_tag="ACG827_001725"
     CDS             complement(6874..7212)
                     /locus_tag="ACG827_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0483867.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7200..7577)
                     /locus_tag="ACG827_001726"
     CDS             complement(7200..7577)
                     /locus_tag="ACG827_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0483868.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7801..8994
                     /locus_tag="ACG827_001727"
     CDS             7801..8994
                     /locus_tag="ACG827_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0483869.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9160..9582)
                     /locus_tag="ACG827_001728"
     CDS             complement(9160..9582)
                     /locus_tag="ACG827_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0483870.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9977..10171
                     /locus_tag="ACG827_001729"
     CDS             9977..10171
                     /locus_tag="ACG827_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483871.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10168..10662
                     /locus_tag="ACG827_001730"
     CDS             10168..10662
                     /locus_tag="ACG827_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483872.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10824..11996)
                     /locus_tag="ACG827_001731"
     CDS             complement(10824..11996)
                     /locus_tag="ACG827_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0483873.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12202..13017)
                     /locus_tag="ACG827_001732"
     CDS             complement(12202..13017)
                     /locus_tag="ACG827_001732"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0483874.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13167..13613)
                     /locus_tag="ACG827_001733"
     CDS             complement(13167..13613)
                     /locus_tag="ACG827_001733"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0483875.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13610..14125)
                     /locus_tag="ACG827_001734"
     CDS             complement(13610..14125)
                     /locus_tag="ACG827_001734"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0483876.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(14198..15112)
                     /locus_tag="ACG827_001735"
                     /pseudo
     CDS             complement(14198..15112)
                     /locus_tag="ACG827_001735"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(15109..17436)
                     /locus_tag="ACG827_001736"
     CDS             complement(15109..17436)
                     /locus_tag="ACG827_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0483877.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            18045..18191
                     /locus_tag="ACG827_001737"
                     /pseudo
     CDS             18045..18191
                     /locus_tag="ACG827_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(18279..18572)
                     /locus_tag="ACG827_001738"
     CDS             complement(18279..18572)
                     /locus_tag="ACG827_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0483878.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            18609..18794
                     /locus_tag="ACG827_001739"
     CDS             18609..18794
                     /locus_tag="ACG827_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483879.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(18975..20153)
                     /locus_tag="ACG827_001740"
     CDS             complement(18975..20153)
                     /locus_tag="ACG827_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0483880.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(20257..20571)
                     /locus_tag="ACG827_001741"
     CDS             complement(20257..20571)
                     /locus_tag="ACG827_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0483881.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            20678..20893
                     /locus_tag="ACG827_001742"
     CDS             20678..20893
                     /locus_tag="ACG827_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483882.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(21193..21435)
                     /locus_tag="ACG827_001743"
     CDS             complement(21193..21435)
                     /locus_tag="ACG827_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0483883.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(21422..21850)
                     /locus_tag="ACG827_001744"
     CDS             complement(21422..21850)
                     /locus_tag="ACG827_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0483884.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         6455 a         4314 c         3705 g         7450 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     5041 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5101 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5161 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5221 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5281 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5341 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5401 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5461 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     5521 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta gtattgcttc
     5581 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat
     5641 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5701 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5761 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5821 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5881 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5941 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     6001 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6061 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     6121 ccagaacaac ggttattata gctgattcat ttccgttctt cttatgtttg taagcgctta
     6181 tttaacaagt tatgtaaatc tctttatttt ttttgattta aaaatgaaaa aatacaaaag
     6241 caagactgtc atacgtctcg cttttgtatt gaaatttctt caattagaga tacttattac
     6301 tgttcaaaag cttctttgaa acggtgaatc aattcattga ctacaggtga taaaatccga
     6361 ttctttttcc agactagcat gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc
     6421 actcgctttg aaagatgact tacagcacct tctatgacta aagcagcacc tacctgattc
     6481 tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc
     6541 ttttttatgc ctaaccagtc agcaaacatt cgctgtattt ctggacgctt tgagatcaaa
     6601 agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa aacagaattt
     6661 tctaaaatga gcaatcccca tttttccttt cttggtaaca aaatcgtttc gtatttctct
     6721 gtatctactg gatctagcaa aatcgctaaa tctaacaggc ttttttctag tcactccgtg
     6781 atatcatcac ttgttccgct gtggatatga aattgaacgc tgggataatc acggatcaac
     6841 ttttctagta atagtgccaa tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat
     6901 cggccgctga ataattgttt ttttctgttc tcgaattctt gagtggtttg attcgttaaa
     6961 tctaaaattt cttctgctcg gcttttttag aaaataacct gcttctgtca agatcatttt
     7021 gcggttttcg cgtacaaaca gttctgttcc tagttcctct tctaactcct tcagttgtcg
     7081 gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct tgagagattg ttccttcttc
     7141 tgcaattgtc caaaaatatt ttagtaagcg taactccata gcgatgctcc ttgatttttt
     7201 tacttgatac attccatttt atttctagat aacacacgat catatttctt gatttttcct
     7261 tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt tcgtcaaaag ttcactttct
     7321 tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc cttcgctaga taggcgaata
     7381 tattcaatca aagactcgat cgataatccg gcatttcgca tacagatcgt aaagtcgacc
     7441 cattttaggt cttcttctgt ataatcacgg attccgcctt ttgtccgatt aactggtgga
     7501 atcaacccga ttcgttcata atagcgcaat gtatcggccg aaatgccctt ttcttcactg
     7561 acttgtttga tattcatctg atcactcttt ctcttaattt cttgccactt tacttatgta
     7621 ttttatttat tctacaactt taagtgtact ccaagtcaat actcatttct attaaaacta
     7681 agctggatta tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt
     7741 tattcttttt tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa
     7801 atgacaacgt atcagcgaaa ggtattatta tcgacatcgg cgggaattgc tttagagaat
     7861 atggatatta tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt
     7921 ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt
     7981 ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt
     8041 ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc
     8101 tttcgcttta tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat gtccttgatt
     8161 tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg
     8221 caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg
     8281 aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc
     8341 ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc
     8401 agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact
     8461 gtccaaattg ccggctattt tgggttaatg aattggctgc cttcaattat gcaaaatcga
     8521 ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct
     8581 ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct
     8641 ttgtttttaa tttgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg
     8701 agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat
     8761 ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcatt
     8821 atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat
     8881 cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg
     8941 gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg
     9001 agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta ataagaccag
     9061 ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc
     9121 agctttttca actgctaatt tccttctatc tggcggcgac tattctttga cgtccgtcat
     9181 ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg
     9241 agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg
     9301 ataattatat gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg
     9361 cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattt
     9421 ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa
     9481 ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat
     9541 tttaattttc ttttgagaaa cttttgttaa aaaagtctgc atattttctc ctcctgtatc
     9601 tactttacta tactacatac aagaaaaaaa acggatacaa atatcatatt gtcacaccgt
     9661 caatagtgca taaacctgtt attatttctc tttttttcct aagtattcta ttgatatgaa
     9721 tcatttaatc gtttattata taaatgtcaa taatctaata ataaaataac gtacatattt
     9781 tattattgca tacttaataa cagaactata gcaaaaaatc ccgtgtatca acacgggatt
     9841 agattaagtt tcctcaatca ctattcataa cacaattatt ataacaagta agtaaacaat
     9901 tgaaaaggac atttattgaa aacggttgtt ttttacttaa ggaggaaatt tagcgaaaaa
     9961 attagttgca ttgtttttgg tcgcaggaag tttgttgagt gggtgtggtg ctactacgtc
    10021 tactactcaa agcacaagtt cagatcaggc ttccagcagc agtaccacga tctccgaaag
    10081 tacggcagat tcatcgaaag aacaagaaca aattgaaaag aaaaaagaag agaaacaaaa
    10141 agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg aatctgcttt gtcacaagct
    10201 gaagcaaatc ctacaaaaga aaactatgca actgcttcag cagctatcca agcgctacct
    10261 gaaaacaaac aagagttgac aagccgtctt gctgcaattg attccacaat caaagcaaaa
    10321 gaagcagaag aagaacggaa aaagcaagaa gaaattgcac gacaacaagc agaacaagcg
    10381 caacaagaag caatcgcaca gcaacaagca gaacaggcga ggcaagaagc aatcgcacag
    10441 caacaagcag aacaggcgcg acagcaatca gcagctgaac aggcctctca gcaggcacaa
    10501 gccgcagctc ctgagcaaaa taatgaacaa actgtctatg tcacaccgac cggttcaaaa
    10561 taccatacac ataaatgtgg aaatggtacg tatacccctg ccactttaag cgaagctcaa
    10621 gctcgtggtt taaccccttg ctccaaatgt tatggaaact aattcaaaag ttatactata
    10681 ataataaaat tcttctgaaa cataaaaaaa tagaagatgg cgttccagat agtaaaccaa
    10741 aaataacagt ttacacgtcc atttcgtcag cttctctctt ttttcattta gtctgttccc
    10801 tctgcttttt tggacatctt cattcattct ttctcaatca aatagtacac ctctttttct
    10861 tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa actgtttcga atgtttatgt
    10921 tttacatgga ttttaagcag gagctcctca tgttgcaagc gatagttcct gccttttaaa
    10981 tcagaaaatc tcactagtcc gtattcatca gaagctttca caaatggaac tttttctcta
    11041 gtaatatagt gtttgccgtt gagcgttttt acagaaaaga ccattccttc gattggtcgt
    11101 ccgtttgtgt tctttacata aaaagataaa ggatagtttc gcttagcgat ggccaaagaa
    11161 tctaaataag cgatgagtag ccaaactaag ccgattgatc caagtagtat accgataaac
    11221 caaataaaaa atttccattt tttccattga tcaattcctt ggatcgcttt ttttgcctct
    11281 tttgcttgat aaggaatacg atgccccgtc acaagcagtc gatgtgtatt gaccatataa
    11341 ggagtacagg taagcaaagt cacgagatct ctgccttttt ggatacccaa agaatctact
    11401 tcatcaggta agaccacttg gattttttct acttgatacg ccaatgtctt tccattgatc
    11461 tggatgaaaa actggtctcc ttttttcaac ttaggcaaat ctgtaaataa ttttgcttct
    11521 gggagtccac gatgcccaga aagaacggca tgggtgtttt tcccgccagt cggataagaa
    11581 ctttcttcca ataatgacgt tcctttttgc aaaaatattt ccgttgtctg atcgaatata
    11641 ggtaagctga cattgatttt tgggatttcg atgactccaa gcatatgttc cataaaaaag
    11701 gcattactag gtaaatcttt taatgctttt gcatcaactg cttggttgaa actgcctatc
    11761 cctggtgata cgttttgctc tgccaactgt tggtttttct ttgtcatttt ttcctgctgt
    11821 ttttttattt cctctgaatt cttattcgat gcttgctttt gataatggat gatcatttgc
    11881 tgggcaagaa aatcattcag tgaatcttct acaaaaggat aagaaaagat cccgatccca
    11941 acgactaaaa tgattgccat gaagatgtcc agtattctta ttctgctttt attcatcatg
    12001 tccacctatt ttctaatatg tatatgaaga ggttgtgaaa tcagcgcctt gacctgagta
    12061 ttagcgaagc aaacggcaaa atagttcctc ctattttgcc gtttgttgag cgaataccaa
    12121 gagaccgacc acttgtcaca acctttctcc ccatttgaat cactatcgtt tatttatcgt
    12181 atgatttttg tttccaatag tttaagcttc tgtttcttga tggcgttttt tgaaatagat
    12241 cactgctcct ccaactaaag cagtacctac caagatgaat acgatgatcc ccataccacc
    12301 agttgaaggc aagattcctt taggttggtt atcgatcgtt gtttcatcag aagttgttgg
    12361 atcatcatct actgtaaatt cagtgtacgc agcttcattc ttaggtaatg cgtagccttc
    12421 tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc agcccttcga ttgaaaattt
    12481 cccttctgaa tcagaaatca atttcacttc ttcccagtca gaacctgtga cttcatctga
    12541 ccaagcatat gggtattctc ctgaggcaag gtttgctatt tttttcatta atttaacagt
    12601 tggatctttt gctcttagaa tagaaaattc tgcatcggct aatttttctt gcgtgttcac
    12661 atcttgtttg atgaagtgga aatcaccgta gttttggaca ttttctacag taatcgtgtt
    12721 atccgtagtt gtattttccc gattgactac accgtttgga ttcccaagat tggcttcatc
    12781 tgcgccatgg atgataccgg ttgtttgttg caattttccg ttttttacta taaaagtagt
    12841 ttctttttgt ggattggtta attgataacc atctggtgct tttgtttcaa ctaaagtata
    12901 attgccgtct attaatccat tgacactgat ggatccttca gtatcagttg tgaatgtcaa
    12961 cgctgactca gcaggatcat agagagcgac accagtgttt tcatcaaaac cggtcacgaa
    13021 ttgattctct gaatttttga tctggaattg cgcccctgct aacttatcta cccctgaaaa
    13081 gttcccagct ttctggattt tgatattgct ttctttgatg acatttttag catataggtg
    13141 aatcgtgtct aaaggtttgt cagggtctag ggtaagctta acacgatatt tggcgacgtt
    13201 tcgacaccgg gctttggtgt ttctacaaag acgtatacag catctttccc atcagattta
    13261 cctggaagag tcaggttagc taatccttca ccagaagtcg ttacacttgc gaagggtgta
    13321 gcgtctgctg gaactgctgt gtctttactt ggatagtacg tagcagtgat gtcatatacg
    13381 ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg tcaattccgt tccgttgttt
    13441 atttgatttt ctggtgctga agtgaatttc aatttatgca aattaaccgt aacattttct
    13501 ggtgtaggtg atacggctgc agaaacctct gcagttccta ggccgagaac gaaaggaagc
    13561 agcatgatag cagttcctaa aaacgtccat atttttgttt tgatgttttt cattttattt
    13621 ccctcctatt gtttcctttt tgttgttttt tataaaacca agctgtccct gttgaaagga
    13681 ctagcaataa ccctaaaatt gagaaacctc caagcatctc ttcgcttgtt tttggaaagc
    13741 ttcctccatt tttttcattt gatggaccat ttgtctgcat ctgaactggc gtttttccat
    13801 tattcgtcgg atttgctttt ggttcgttga tgatttccaa tggtatagga tgaagcggat
    13861 cataactgcc tttttcaact tcaaagagaa tggttgattg actcaatata tatcctttag
    13921 gcggtttaat ctccactagt tcgtaacttc caaaagacaa tccatggaca gaaaatgttc
    13981 catctttact ggaagtaaaa atcgttgctc ttgaatcatc tttggctacc caatgattta
    14041 tcccgttttc ttgcgctaag tattggtcat tttttttgac tagaaattga gcatctgcta
    14101 gtttttgttt cgtttgccga tccactttga caaattgttt gcctcctgtt tggatggcaa
    14161 gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac atcgttatca aatgtcgttt
    14221 tttcactgtt ttctttcaat gtcatttggt aacggatcgt taattttttt ccagcaaacg
    14281 aactaagatc ttttccagaa tctgataaga caaaaccatg ttttattttt tgtgtgtgaa
    14341 atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt ttctggttct agccataact
    14401 gaggatcagc tgtatctttg atcgtatagg ttcctatctc gttgatgttc tcaggaattg
    14461 ttacggtgat gagataaggg actttatccc catctgcaaa atctgtctgc ttattctcta
    14521 ctgtttttgt taatgtgagt gcagcaggta ttttttgttc gctctttgga tacaagtgga
    14581 tggtcgtcaa tttatgatta gatgaatctg tcacaggaag cacaacgaca aatggtgctg
    14641 atttttcttt tacttaatct gaagtcttta tttccgtaaa tcggtaaaca gcgtcacgac
    14701 cttgctcatc tttgtccgat gcactaaaag aagcaatgcc ctcttcctcg tctactgttt
    14761 ttgtcacatt ttctgccagt attttttcac tatcactttt ttgtgctagt tttctttgtg
    14821 cgtcttccac actacttcct tctgaccgca gcttgtaaaa ttcatccgag acatcatata
    14881 tagcgaaagt aacgccgttc aaccctttta ctccttgaaa gttatccggg atatttattc
    14941 cgtcgttttg actgatactt ggcatcttat cgctttcgaa cattcgatta tgcaaagtaa
    15001 atgtaatagg tgattctcta gctgacacag tcggagaacc tataaaaaag accagtagga
    15061 taaaaaagag aacagcaaat atatccacac gtacattttt tgtttttttc atcgctgttc
    15121 acctgctttt ttcttacgat ggataaaata aaagaagcca gaaaaaataa agatcagtcc
    15181 taaaaagaca aagggcagta gacctatccc acctgtcgaa ggaagcaaga gagtcggatt
    15241 gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa ttattgttta cacttccttc
    15301 gcctaaacta atcgttacta tttgtgatcc aatatctgtc cggctgttaa ctgttagggt
    15361 ataatccaat gattcgccta agacaacacc ggtaacgtgg agatttccaa ccgaaatcga
    15421 gtgactttgg gcatcataat taaaggcagc gtcatcgaca gtaatcactg cgttagcttt
    15481 atagatggta taagttcctg atgtgttggc tatatcagta aatgacaact tgggatttgc
    15541 tgctttgact ttgtctattc cgctattcgt tagtttaatt gtatagctgc cagcattaac
    15601 catatttttt gctgattgtg catctgctgt gtaatccata ctgctaagtg ccacttgtac
    15661 tggagtaagc gaactagtga caccctctgc tagcttgaca tggattgacg ggacacctgc
    15721 ttttccatca tatgtcttac tgccactgag ctctaccgca ccgctgattt gcttaatcgt
    15781 atagacatca gatgtctgca cgatagtaaa taaatagttt gttctggcag ctagtttagc
    15841 tagtccgctt tttgtcaatt gccaagacgt ataggttcca atttcagtag gcgcagttac
    15901 ttcaactccc ttttcattca tcgctttgaa atcagttgga ttccatgtga tttgatcaat
    15961 actgagatca ttgtttaaaa gggatgttga ttctgtttta tcattaaagg catatttaac
    16021 gatcggtaca acagaaagac catcatagta cttactgcca tttgcattga ttgatactgt
    16081 tgcatttgtc ttataatata ggatgacttc agaaatctgt tttccatttg cgtcctttac
    16141 gattacattt ccatttgtat ctaaactagc taaaacactc attgctgatt ggttctttgt
    16201 ttctatcgct gaaacagctg taaaggtata tccgttgata gtaggcgtat tagtcgtcag
    16261 gtcttgtgca gcacttccgt aagtaagatt cattatttta gaaggagcaa tcatctgtcc
    16321 aaaatagtct tgatacttaa tagtgattgg tgtttgtttg cctgtatagg tcaccgtatc
    16381 gacgttactt tctagggctg tgaaagaacc gtttgtttgt gcaaaagaat agcccgttac
    16441 tgctgttgga gtgataacca ctttgtcacc cacatggatt ccagataatg tagtcccgta
    16501 cgttccgatt tttgctgctc caccattgac tgtataagaa aaagttcctg tccgattcgc
    16561 attgacctga accgttgctg cttgataggt tggaaaattt gaaacatttc ccttaccatc
    16621 aacgatctgc ccatttttga tcgagtaccc cgtgaatgga atatcatttg ctaactgtat
    16681 accgattgaa aaagtactca tacctcctcc ggcaggaagc cctccccatg ttgagggtgt
    16741 aaaagaataa cttttatccg cattgggtgt aactgaatcg atcgtatttc cagcggtagc
    16801 cacccccata tagatacttt ttgggaatgt gatagtagta ccatttactg tggagccaat
    16861 aaacagatta tggatatctg gacggttatc aataaaagcg attgctttgg ttacgccttg
    16921 taacgcagtt ttatccgcac tcatcgatcc gttaaccgca acaatgactc catctttggc
    16981 atatatcact gtatatccat ctggtatggt atgtccgttg aaattcgcag tggccgcaac
    17041 agtactaaat gtctggcttc tacctgtttc tgaataagct ttctcccctg ctgcttttgc
    17101 tgctgtgatt tctttttctg tcggatcaat cagttgagtc gaggatgtgg ttgaggttgt
    17161 ctcgttgttg ctagactgat tggtgctttc tatggactgt atttcagaat agggtggact
    17221 atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt tctgtataaa cgtttacata
    17281 aatacttgat aagctcccta agaaagtaaa aatcaagatg atcgaaatca taatgggatg
    17341 taagtatttt cttttagata cagctttgag tctagaaaca aaatgttttt gacatatacg
    17401 ttggatttta ttttgagttt tcttaatcat tttcatccct cctctcaata aaaaatcatc
    17461 aaataatcct ttacacgtta ttctaaattt aatttttatt attttattga tgtatagaaa
    17521 gtttggggct ttttttctct ttttattcta aggttatttt agtcttgtcc tttaatacaa
    17581 agattttatt tttaaataaa aaatactctt tttttacgat tattgaaaaa aaaagataaa
    17641 acttgattaa ttgaagtatt gcttaaattt ggaagctatg agggaagaat aaaagagtta
    17701 ggacgggatt atctactatt tttctttgtt ttaaatattt gttatctttg aacataaaag
    17761 agaaatgtgt cgactgcagt gttgtgtccc tcatcacgaa taaacggtgt tcaactagct
    17821 gatcttgtat tcacttaaaa aatggtaaag tacgagttgc tattccccat tttcaatttt
    17881 ctaacgctca ggacaaaata ctgttttcac aacctcattg gaataaaagc atatatattg
    17941 tttcaagaac gaaatcttgt tatcttttta aagggcatct gtccaaaatc taattttagg
    18001 aggtacgttt tggtatttat tcgtacaaat aaaagagaat tatctttatt catgtaacgc
    18061 atccaattga ttttttcatg tacgctcctg gaacgatttt actgacgatt gcttatttaa
    18121 ctgcgaatcg ctcattagct tttatcatag ctatccatat tttgaataac gttcttggct
    18181 ttgtattata gatacagatg acaaaaaaag ttcaagtatg cggtgtagaa ccaatatcgc
    18241 atacttgaac ttttttcaat gagtataaat taaaacttct aattttcaat tcccatcatc
    18301 tgatatgccc gaccgtctga acttcttttg aaaaagccaa atgtgattag ataacgtctt
    18361 tgttcgacat aatcgccact agaaaatacg ttcagcagta tcgaattaac ctctgtttct
    18421 gtataagtca ctccatggtc aaagcgtttc aagacttctt tcaataggat gtatttcttt
    18481 tttgttgaac ttggtacttg ttttactttt ccatcattca tgaatgtgtc taaaactttc
    18541 atcttttctt gctccgtata agtataaacc atcttctttc tccttttgat tctaagaata
    18601 ctccatttat gttagaatat tctggatttt ttattaaagc ggttactctt aagaaagata
    18661 ttatcatagt gctctttttt ttactactac ttataggtag atttattcta atccattatt
    18721 ctcccctacc aataattaaa gaaaaaactg gatcagggtg ttcaaattac atgctaagaa
    18781 aaaagaaccc ttgaatacca agggttcttt ctcagtctga taacaatgat taacgacgga
    18841 tttctttgat acttgtgaaa agtcgttaaa gtattgatat aaaaggattt ttattacctc
    18901 tgactacata ttgactaatt tgttaaaaaa tcgtttttca agaatcctgt aataccagtg
    18961 ctttcaccgt ttttttaact ctctaaagaa ctaaaagcaa tcagaggttt taccttttca
    19021 tttgacgtta agtggcgata aattttcgaa gttgtttggt cagtatggcc taagcgatct
    19081 ttcaagactt cccagctaca gtgttccgct actaagcttg ccattgtatg acgagctaaa
    19141 tgagtatgaa atggcttatt aattcctgag cgattcacgc accgtttgac gtgatcattt
    19201 acatactctc gacgataagg aacaccacgt ttactagaat caagatacac aaacaaatat
    19261 gtttcttcta taataaacct gtaatgttgc ctcatctcac ccaaagcctt aatcaactgt
    19321 tgtagctttt ccataactag aggagttact agaactcgtc tagcagaact aactgtctta
    19381 gtagtttcaa ttcgaaaatc cttactctta cctttaactg ccgtttgctt atagaacaga
    19441 atttcatttc ttttgaaatc tatatctcca ggctgtagag caagcgcctc gctgactcgg
    19501 cagccagtgt aaaatagaac atggaaaagt acagacatcg gtaattcctc atgaacaatg
    19561 ccataattca aaaaagttct caattcatcg acagtaagaa acttgtcctc aatattattt
    19621 atggcttctt ttaaatcagt taccgttaac tgatattttg gcattctcac agacttcatt
    19681 ggattttcac ttatcaaatt ctttctttga caatatttaa aaatcatatt tagaacttga
    19741 ataagtgatt gtaaactatt tttggcatag ccaacatttg ttccattaac gccacattcc
    19801 tttaaggtaa aaagcaattc ttgaaggcac tctggtgtca aatcagacaa ctttttatca
    19861 ccaatccatc gaacaataat ttttaattgt tcctctcgct tatcataggt tatttccttt
    19921 tgttcattta acgaacggta atattggaac cattgctcac ctacaacact aaacttgtca
    19981 ttgcttgcaa caattttttt cttaagcctt tgttgtttct ccatttccag ataagcgagt
    20041 tgcgcctcac gttttgaatt aaagccacgt cttgttgttc gatatgcttg aaagtaccat
    20101 gtgatcctac cagtagaact gatttgcttc ttgaatcgat ctgttttttc catttattga
    20161 ttcctcctat tttgaaggaa cccaacaagc agaaaccctt tggaaatctt tctatctatg
    20221 tcatcattgt accgcatctt agaaagaaaa tgtaacttat aaagatcgaa gtagtcgcaa
    20281 tttttctcga tagtcattcg aatcactctt tctcatccat tctaaaacat catcacgaaa
    20341 atagtgtcct cgaatcccgt accaacaggg aggaaactca ccaagtttta ctaaacggtt
    20401 cagtgtacta tcacttatct gaaatagtcg ctttaaatca gtatttgtta ataccagagg
    20461 taaatgactg actgcaagca atttttccgt gtaatcttcc agtttttttt gtaaaattga
    20521 atagaactct atttccagag aattaacatt tgttttaact tccaactcca tcacccttct
    20581 ctctcaaaat caaaacgacc accacggtac cccttaaaat tgtaaaaaaa cactccagta
    20641 ctccatcaaa acggactaga aaagaggaat ttttaatatg aaacaaggaa ttgcagacat
    20701 aaaaataatt aaagaaattc ttgaaaaatc tacggctaat gcaatagcat tcggaacagg
    20761 aatcaacttg agtacagtaa aaaaacttaa aagtggagag agagctgaag aaaaattaaa
    20821 tcttgccgat gctattaaaa tcacagaatt tggcatgaaa aatatgccaa ccaaaatcga
    20881 aatatggaaa tagtagtttt tctgatctat cagctgtttt gccaaagcac ataggaatct
    20941 cacctccccc cagtaatggc gggccgattt ggttacggaa gtatcattat cgtagtcgcg
    21001 attctcatat taaaatttgt cgcttaaaag ctaaccttgt catggcgtaa ctagagaatc
    21061 aaaatctaca tgtactgata gatttgctat tcatttgtca aagagcgcta ggctattttc
    21121 agcctatgga aatcctttat tttgacccga taaaaaactt ccatagaatg aaaaggaaag
    21181 ggaaaaaata ctttaacgag ttgtatcaaa ttcaagtatc ttctcaatca attttgtttc
    21241 cagttgtcta cgcatatatt catctacata cataaccatt cctttttctt cttcataaac
    21301 tggtttcatg gctaaccgtg aaatataatt ttgatatttt gacagaacga tggacatggc
    21361 aattgaatca ccagaagctg cggctttgat tgtcgttagt ggtatgaatg gtatatatcg
    21421 tttattttgc ttcatatttg acacccaaat actttcctag ctcttccaaa gccatttgtc
    21481 gttggtacca aacactgccg agacttttcc caataatatc gccaatttca cgatcattaa
    21541 gaccgatgaa gaaggacata aaaatgattt cttgtctttg actaggtaat gctgcaatcg
    21601 catttgccaa ctcctcattg gaaataaaga ttttcatacc aaggacaata aaagtaccac
    21661 tactattcaa atttggttcc aaataataga actctgtacc taaccctttt tgttccaata
    21721 tttccattga tatttgccgt tggcgaaatc tagcgtactt cttttgaata tttaacgcct
    21781 cattgcagac tactttctta caaaaactgt caaacgtatg ttgaatgtgg gtttcctctg
    21841 agtcgcccat aatattctcc ccctcaaaaa attcattcaa ctcgttttac ttctatccct
    21901 ttccattatt actacaatct agga
//
LOCUS       DAZKOU010000050        21834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000050 DAZKOU010000000
VERSION     DAZKOU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.050"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACG827_001745"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACG827_001745"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0483885.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACG827_001746"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACG827_001746"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0483886.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACG827_001747"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACG827_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0483887.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACG827_001748"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACG827_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0483888.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACG827_001749"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACG827_001749"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0483889.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACG827_001750"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACG827_001750"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0483890.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ACG827_001751"
     CDS             complement(8054..8524)
                     /locus_tag="ACG827_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0483891.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ACG827_001752"
     CDS             complement(8570..8995)
                     /locus_tag="ACG827_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0483892.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ACG827_001753"
     CDS             complement(9062..9331)
                     /locus_tag="ACG827_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0483893.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ACG827_001754"
     CDS             complement(9342..10937)
                     /locus_tag="ACG827_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0483894.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACG827_001755"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACG827_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0483895.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACG827_001756"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACG827_001756"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0483896.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="ACG827_001757"
     CDS             15403..16377
                     /locus_tag="ACG827_001757"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0483897.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="ACG827_001758"
     CDS             16547..17947
                     /locus_tag="ACG827_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0483898.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="ACG827_001759"
     CDS             complement(18025..18747)
                     /locus_tag="ACG827_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0483899.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="ACG827_001760"
     CDS             complement(18826..19332)
                     /locus_tag="ACG827_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0483900.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="ACG827_001761"
     CDS             19455..21254
                     /locus_tag="ACG827_001761"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0483901.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..>21834)
                     /locus_tag="ACG827_001762"
     CDS             complement(21322..>21834)
                     /locus_tag="ACG827_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0483902.1"
                     /translation="TTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPV
                     TPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGW
                     TVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQ
                     WFAPDFAVSM"
BASE COUNT         6049 a         4791 c         3964 g         7030 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agctttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt cccctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggaa
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttacac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggttgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgt
//
LOCUS       DAZKOU010000051        21621 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000051 DAZKOU010000000
VERSION     DAZKOU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.051"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..457
                     /locus_tag="ACG827_001763"
     CDS             <1..457
                     /locus_tag="ACG827_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0483903.1"
                     /translation="KFEYRYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEV
                     PVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDT
                     LMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
     gene            472..1224
                     /locus_tag="ACG827_001764"
     CDS             472..1224
                     /locus_tag="ACG827_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0483904.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1927..2142)
                     /locus_tag="ACG827_001765"
     CDS             complement(1927..2142)
                     /locus_tag="ACG827_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFQ0483905.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2161..2397)
                     /locus_tag="ACG827_001766"
     CDS             complement(2161..2397)
                     /locus_tag="ACG827_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0483906.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2655..4808
                     /locus_tag="ACG827_001767"
     CDS             2655..4808
                     /locus_tag="ACG827_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFQ0483907.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4777..6192
                     /locus_tag="ACG827_001768"
     CDS             4777..6192
                     /locus_tag="ACG827_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0483908.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6225..7259
                     /gene="sppA"
                     /locus_tag="ACG827_001769"
     CDS             6225..7259
                     /gene="sppA"
                     /locus_tag="ACG827_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0483909.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7261..7782
                     /locus_tag="ACG827_001770"
     CDS             7261..7782
                     /locus_tag="ACG827_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0483910.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7779..8165
                     /locus_tag="ACG827_001771"
     CDS             7779..8165
                     /locus_tag="ACG827_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0483911.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(8661..8733)
                     /locus_tag="ACG827_001772"
     tRNA            complement(8661..8733)
                     /locus_tag="ACG827_001772"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8698..8700),aa:Lys,seq:ctt)
     gene            9114..9683
                     /locus_tag="ACG827_001773"
     CDS             9114..9683
                     /locus_tag="ACG827_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0483912.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            9670..10530
                     /locus_tag="ACG827_001774"
     CDS             9670..10530
                     /locus_tag="ACG827_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0483913.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            10720..11424
                     /gene="yycF"
                     /locus_tag="ACG827_001775"
     CDS             10720..11424
                     /gene="yycF"
                     /locus_tag="ACG827_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0483914.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            11429..13264
                     /gene="walK"
                     /locus_tag="ACG827_001776"
     CDS             11429..13264
                     /gene="walK"
                     /locus_tag="ACG827_001776"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0483915.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            13261..14577
                     /gene="yycH"
                     /locus_tag="ACG827_001777"
     CDS             13261..14577
                     /gene="yycH"
                     /locus_tag="ACG827_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0483916.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            14578..15447
                     /locus_tag="ACG827_001778"
     CDS             14578..15447
                     /locus_tag="ACG827_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0483917.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            15502..16311
                     /locus_tag="ACG827_001779"
     CDS             15502..16311
                     /locus_tag="ACG827_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0483918.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(16414..17043)
                     /locus_tag="ACG827_001780"
     CDS             complement(16414..17043)
                     /locus_tag="ACG827_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0483919.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            17213..18505
                     /locus_tag="ACG827_001781"
     CDS             17213..18505
                     /locus_tag="ACG827_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0483920.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(18581..19243)
                     /locus_tag="ACG827_001782"
     CDS             complement(18581..19243)
                     /locus_tag="ACG827_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0483921.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            19468..19752
                     /gene="groES"
                     /locus_tag="ACG827_001783"
     CDS             19468..19752
                     /gene="groES"
                     /locus_tag="ACG827_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0483922.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            19803..21428
                     /gene="groL"
                     /locus_tag="ACG827_001784"
     CDS             19803..21428
                     /gene="groL"
                     /locus_tag="ACG827_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0483923.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7390 a         3485 c         4352 g         6394 t
ORIGIN      
        1 taagtttgaa tatcgttatt taaaaaatat aaaaaatgaa tatttgaaaa gtttgttgat
       61 agcgaagcta taccaagcaa ataacaataa gatttgttgt aaattcgaat gtccaagtac
      121 tattattgaa gttcctgttc caatatttga ctgtattagg gtgcttggaa ttattctcga
      181 taatgcaatt gaagcatcta tagagagtca agatagagaa ctgtctctag ctatttaccg
      241 agatgataaa cagatagaaa tttcgatatc gaatagttgt agagagatac tactctctat
      301 agatactcta atgaaaaaag gaataacaac aaaaaaggga catcaagggt taggtttatc
      361 tagtatagag gaaatcaata aaaagaatcc caatatgttt gtgaactatc aaaacgatcc
      421 gtatcaattt actactcaag ttattttgtt gttttgaggg aaggaggatt tatgagttat
      481 ccaataatat tatgtgaaga tcaaattata caattaaatc agttagaacg aattattgat
      541 aatttcattc tttttcacga taaagtattt aagatagtat tgaaaacaca aagtccgtta
      601 gaagttaaaa aatacctcaa acaattccga ccaaagcaag gaatatattt cttagatatt
      661 gatttaaatc atgaagttaa cggtatagaa ttagcagaag taatcagaaa atatgatgtt
      721 caagcaaaaa tcatttttac aactactcat gatgagatgt tacccgtaac aataaaaaga
      781 agagttgaaa cgttaggatt tgtaacaaaa gatcaaacac tagatgagta tcgaaacgag
      841 attgttgagt tattgttatt agcgcaagaa aggatagatg caacaaaaca ggttacgaat
      901 caagcatttg ttttttccat aggttctcaa acatttactt ttgatataaa tgacatttat
      961 tttgtagaag cttcaaaatt accacataga ttatcattgt gtactaaaga tgggcagtac
     1021 gaattttatg gaagaattag tgagcttgaa aagaaatatc ctatgttaac aagaattagt
     1081 cgagcgtgtt tagtaaatat ctttaacgtt aaagaaatag attttaaaaa acgttcacta
     1141 tattttgatt cagagttggc cagaaacttt actttaggga aagcacaaaa aattaaggag
     1201 aagttgaaag aaagcactgt ttagtggttt tttctttagg gagattgcat ttttctaata
     1261 aaatagattc cttcactagt gcaaattttt ctgaatccta ttaaagttgt accaattttt
     1321 atagctgtta ttagttacgt gcataacagc aatattagtt actggaggaa tatttctatg
     1381 gtctggaacc aaaaaagggc caaatgaata gttcttttct tgtttttatt aagtaccata
     1441 aaggggaatt aaaattaaat gtatggtgga agatgcgaaa gatttggcaa attctactaa
     1501 tttttagaaa gtgtttttca ttttttagtt aaaaaatttg tcctgtcaag tgcaatccta
     1561 tgattgtact tgataggatt tgagataatt actttcctgt ttttataaat ttaacttgca
     1621 ggaacaggct ttaatgcaca tccgacaagt acattaccaa tacctcctgt aacggccgaa
     1681 ataggtcctc ctgcagcacc aactacagcg ctgcaagtcc ttttgtaaaa atatctctac
     1741 cattacattt ccaaagaatt tttatctgac tttaataaaa gataaaaatt taatataatt
     1801 ataaaaacta gcacaagaag ctttagtaaa aagttctgtc cattccaaac ttttgaaaag
     1861 ataaagaaca tacctatgtt cgtaatcgac acaaaccaat cttgaggttt gatatttttt
     1921 aattgtttac gcaccggtcc acatgctgaa tccagcacct aataaagctc cacctacacc
     1981 acaagcagct attgcataag gtcctccggc tgaacaaagt tttactcctg ctgttgcccc
     2041 agaaaaagta gtgcctaaaa ctcccacggc actcccacct tgaatatttt tcaactcttc
     2101 tgaagataat ataaatgttg tttttgtgtt tttaatatgc atactactta ttctcctttt
     2161 ttattttttt cgagcatccc agccagctgc agcacctcca gcgacagctc catataaccc
     2221 tccaacaaca ccgcaagctg gtccgcaaat tgctgttcct aaacctgcac cacctactgc
     2281 ccctgtggta agtccaccta caacttgccc caattttccc caagcattat tgcctccacc
     2341 aacagtttgt gcatttccac aatacttagt tcctttacat tttgcatgtt taaacacgct
     2401 tctttaaata atatttttta cggtgctact gtaacatacg tatttgatta acagtaaaaa
     2461 tcttcctgaa acttatcaag tttattcttg aatagtaaaa tttccaaaat aaatttgatt
     2521 caggaatgaa aaagttagtg ttcaagaact gttttcaaag tttttgtgga attatgagac
     2581 aatattttca ctgtattaaa gctatattga gaatttttct ataaaaattt tttagaagga
     2641 gttgtgttag ataagtgata aaatcatttc cttttgtgca gcaacaagat gaaaaagatt
     2701 gtggtgttgc ttgcctttcg atgattttaa aatattttaa aactgatatc ccgattcata
     2761 aattacggga cctatctggg actgatttag agggtacttc agcatttgga ttgaaaaaga
     2821 catttgaaaa attgtctttt gattgtatgg ctattcaagc agatagtgag atctggaaag
     2881 aaagagatct tatcttacca ttaattgcac atatattggt tgataacagc tatatgcact
     2941 acgttgtaat ttataaaacg aagggaaacg tactatggat tgcagatccc gctaatggaa
     3001 aaaatagaaa aacgattgaa gcgtttcaaa aagaatggac aggtattctt ttattaccta
     3061 tgcctagaga aacttatatt cctattaaag aaaaagtagc tggattaagt tcttttttgc
     3121 ctataatttg gaaacaaaaa ggattagtat tccacatcgt tttagcatcg attttcatta
     3181 cgttttttgg cataggaagc tcatattatt ttcaaggtat tcttgatttt ttcatcccta
     3241 atcaagcacg ttcaacttta aatataattt ccattggctt gatcgctgtc tatttatttc
     3301 gggtattgtt tgaatatagt cgtagttatc ttcttattat tttaggacag cgaatgagta
     3361 tggcaattat gcttcagtat ttcaaacatg tgttaacttt accaatgaat ttttttgcta
     3421 cgagaaaatc aggggaaatt atttctcgtt ttcttgatgc cagtaagatt attgatgctt
     3481 tagcaagtgc tacgttatca atatttttag atattggtat ggtgttgctt gttggggtta
     3541 cgttagctat tcagaatagt acgttgtttt taatcactct atgctctttg cctttttatg
     3601 tagttgcaat attcgctttc gtgaaaagct atgaaaaatc gaacagggaa gaaatggcag
     3661 ccggtgcagc tgtaaattct agtatcatag aaagtcttaa aggaatagaa acaattaaag
     3721 catatagcgg tgaagagaaa gtatataacc gtatagacca tgaatttgtt aaattaatga
     3781 aaaaatcatt tcgtacggtt acgcttgata atattcaaca aggaattaaa catttgattc
     3841 aattaattag cagtgcatta attttatggc taggttctta ttttgttatg gataatagaa
     3901 ttagtctagg tcaacttatc acatacaatg ctttactagt atttttgaca gatcctttac
     3961 aaaacattat taatttacaa gtgaaaatgc aaacggctca agttgcaaat aatcgtttaa
     4021 atgaaatttt cgcgattgat tcagaaaaat cgaaccagtc ttttgaatat atgctttctg
     4081 aacaattatt tcaacaagat atcactgtaa aaaacgtttc attctcgtat agcatgaagg
     4141 ccccaacgtt gaacgaaatt tcatgtacaa ttccttcgca cagtaaaacg gctatagttg
     4201 gagttagtgg ttctggaaaa tcaactcttg caaagttatt tgttcgtttt tatgaagcat
     4261 cagaaggatc gatccattat ggaaacatta atcacttaga cattcctttt cagatattac
     4321 gcgatcgagt gacatatttg cctcaagagt catttttctt tagtggaaca atcattgaga
     4381 atcttatatt cggtttagat agcccaccaa gtttcgaaca aataatagaa gtttgtgaga
     4441 tagtccaact aaatgaattt attaatcaac agccattacg ttacgagaca ttgttggaag
     4501 aaggtggaaa taatctttca ggagggcaac gacaacgatt agccttagca cgggctttat
     4561 taaaaaatcc ggacatatta attttagatg aagcaactag tggattagat accttgttag
     4621 aacatgcaat tatgaattat ctcctcaatt taaaagaaaa aacgattatt tttattgcgc
     4681 atcatttacc aatagcaaaa gcatgtgatc aaattttagt tctccatgaa gggcatttgg
     4741 tagaaaaagg aacgcacgat gaacttcgtt ttgccgatgg tatctatcaa aaattatggg
     4801 aaatctagga gagggaaaca atttatgcag aataataatt ggttcgaaca ttctagtatc
     4861 tatactaaac aacatcatag cttctaccgt tgggtgttat accctgtaat tcttttttta
     4921 tgtattattg gattgttttt aacctttgct aaaaaagagg ttgttattcg gacaacggct
     4981 caattgaacc ccgaaaaaat agagaagtta caagtgccat tagaagctaa aataatggaa
     5041 aacttcctaa aagaaaatca atttgttcat aaggacgatg tgttggttag attggattgt
     5101 tctttaatag agaatgaaaa ggctcagata gaacaagaaa accaacgtat tactcaacaa
     5161 ataaagatgg ctcagctatt tattgaaagt ataagtaaag gaaaaaattt gttttcaacg
     5221 gatgacagtt ttggctacag taatcaatta aagagcatgt tgtcagaaaa agaatcactc
     5281 cgctacgctt tgaagcaaag tgaattaaat gatcaaaagc aattagaagt atacgaaaag
     5341 acaaaaagac aactagaaaa acaaattgag agttcagata gtaaattaca agaatggcaa
     5401 caagtacagg tagcttggag taataatcaa tcattaaaag atttttcaaa agaaatgatg
     5461 gcaaactatg agaattggca agaacaacta aataatgttt ctgatgatca aaaaaatcaa
     5521 gtgaaactga caatttcagc aagcataaat gaacaaattg agcaactaaa aaaagaagta
     5581 gaacagtatc agtcagaaaa agctaaatta gttaaaccaa ctacttctga gaatgacaga
     5641 attagtcaaa cggaaaaagg aaagcaagag ctagaacaag caataacaac tgtaaaacaa
     5701 acagttacag agctaaatga aaaacaagag aagaaccagt cagttatcaa aagtttggac
     5761 gatcaacttt caaaaggaat actaaaagct cctgtatctg gaacggttca tttaaatgaa
     5821 gaaacaaagg gacaagcaga actagccaaa ggaacagtgc ttgcagaaat ttatccgatc
     5881 cacgaaaaat caaaaatgaa atttacagca cttttacctg cgaatgaaag tacctatatt
     5941 aaagaaggga tgaaggttca ttttaaattg gatcaaaaag gtggggctcc gattacgatc
     6001 gacggaactt tagatgaaat ttcagaaaat tctactgcca ctgagaaagg tgttttttat
     6061 gcagtgaaag gaacgttaaa aacaactcaa tcgtttccgt ttcgttatgg attaactgga
     6121 gagttgtctt taattgtagg agaaaaaaca tattggcaac aaattgtcga tgtgtttcta
     6181 aataaaaaat aattattttt actaagaata taggaggagt ttatatgaat aaaagaagat
     6241 ggattgctgt gggagtagcg tgcggattat tgttactatc cattatcgtc gcgctaattc
     6301 caggaaaaga taaagaggaa gcatcaaata caaccctaac aggaataaat aagatttttt
     6361 acggatctaa cgaaatcaca gaagaaacgt tagaagaagg cgcatcaaat aaaaagattg
     6421 tcaaactatc cgtcaatgga gttattgcag atacaggtga aagcaatctt tttagtaggg
     6481 aacaatataa ccatcagaat tttttaacac agctaaagaa aattcaagaa gacaaagcgg
     6541 ttaagggagt gttattagaa gtaaattcac ctgggggagg aatatatgaa agtgctgaga
     6601 ttgctaaaga aatggcaaaa attaaaaagc tagatattcc tatctatact gcatttaaaa
     6661 atacagcggc aagtggaggg tactacattt ctgctggttc agataaaata tttgctacgg
     6721 aagaaacaac aactggatca attggtgtca ttatttctgg tttaaattat tctggcttat
     6781 tagaaaaact tggcgtaact gatgcgacgt ataaaagtgg tgcattaaaa gatatgatgt
     6841 cacctcaaca caaaccttca gaggaagaga ataaagttat acaggaattt gtgatgagtg
     6901 cttatgatcg ttttgtcaat gttgtagcaa aaggaagaaa tatggatact aatgcagtga
     6961 aagaacttgc ggatggaaga atttatgatg gtaaccaagc agtagaaaat gggttggtgg
     7021 atcaaattgg ttactctgaa gatgcattag attccctaaa aaaagagaag aaattaactg
     7081 atgcgacggt tatagaatat aaaaatgaca caactggctt tgcttcttct tggttaggta
     7141 ataagattgc tgaatggcaa gggctaaagg caactacatc agatcgtgta atttcagttt
     7201 ttgaaaaact tggattttct gatactccaa aaccaatgta ttactatgga gggctataaa
     7261 atgaataaga agagtggcat tgaaactttg cctaattatg tatttgcggg attttggtta
     7321 cgattttttg cttttcttat tgatttaatt tgtattagca tcatcaccca tgctactata
     7381 ggtctctttt acaaaatcat gggactggaa tctgacaaat ccattattac tttttatggt
     7441 atagcgtcat tagctattta tttaacctat tttgtacttt tgactaagct gaattcagga
     7501 caaacaattg ggaaaatgat ttttgggata agagtagtga gctttgagga aaaagagcta
     7561 agttggcaaa ctatctttat tcgtgagtgt atatgtaggt ttatattaaa aaattttgta
     7621 tttcaaattg ggtatttggt ggctgctttc acgcccaaga aacaacatat tggggattat
     7681 ttttctaaca cgtcagtggt tgttattcgt tcattagaag ctattaacta tggtcaaaaa
     7741 atttctaatt ctaaaacact taatctggag agaattaaat gaataagaga aaaacagagt
     7801 ttattttgat tttaattagt ggaataacag ggattttatg tagtctttta gcattgatgg
     7861 gtggtatgtt tctatcttct attgattcaa ctactttaaa tactacagaa ctaaccagta
     7921 aggatataga tatgttagtc aatctaagcg ggaatgttgt tttatttgga ggaattaccc
     7981 tagttttttc gataatgatt ttcataatag gttttttagt aagaaaaaat caaaaaacag
     8041 tggtctatgg tattagttta ctcattttat ctattattcc attttttctt ttaacattat
     8101 tgtgggtaat tcctggagtt ttaggtatta ttgcgggatt gatgcttcta ataagaaaat
     8161 cttaattagg caatgtgtag acaacgtgaa acgattaatc aattaaaagc taaatacgtt
     8221 ttgagaaatg ataacttgta gtcgatggaa gacatgtaga taaaaaaact acgtgtcttt
     8281 tatttattct aattttttcg aaaaaaagac actctctgat tgttgtatta atggaaaagg
     8341 ggaaagcagc aagtaaattt agtttttcta ttaaatattc ttcacttttt aaccttttat
     8401 agtacttaga tttaatccta tatgtttaga gagttcattg gcagcatgat tttgcaaagc
     8461 ttctcgaatt attgttatat tagaaacttt actgttcata attatgcttc ttttttaact
     8521 caaagtccaa agggctacag tggactttga gttaaaaata aaaagcccta gttattttga
     8581 aaaataaaaa aagaacagcg taaatgtttt cgaaatgctt tcgcaccaac atttacctgt
     8641 tccgtgggtt tggcacccaa tgggccgtga ggggctcgaa cccgcgaccc gctgattaag
     8701 agtcagctgc tctaccaact gagctaacgg cccgtacaaa aagtacttaa taatagtagc
     8761 actaagaatt ttaaaatgca agactacatt taaagaaaat cagaaaaatc tttacttcct
     8821 tataggaaat cattcataga ttattcacat ttgctcgcta ttatataagc ataccaacaa
     8881 acaaagttct tgcaagagaa caaaccctaa cacttttttc attttttact cctttcccgc
     8941 cgtctccccg gcgggtctcc tgttcttaac gataaaagac aagaaggctt agccttcttg
     9001 ttttttgttt tcatggaatc ccgctaatca aatcacctag ttaaagcaac tagattgaat
     9061 gtttatcata agtctgatat catgtttatg aaatgaaaaa aggagcggtt atgatggaaa
     9121 aaatgcgtca gcaattaatg gagtggggga aggaactaga aacattccgt ctgcctcact
     9181 gggaagaact ccctgactta gaactttata tggaccaagt cagaacgtta gttgatcgtt
     9241 atctttcacc tgtgatacaa ggagaaaagc atccattgct tacctcttct atggtaaaca
     9301 attacgttaa actaggtttg ataccagcac cagttaaaaa aagatataac aaagaacacg
     9361 tagcattttt acttgcaatc actacgttga aacaagtatt gactattcct gaaatcaaag
     9421 aagggatatt gttccaagga aaaacagtag gaattcgtga agcctataat ctattttgcg
     9481 atgaacaaga agcagctgtg tggatggtca gccaattagc acaaggtaaa tctcatccgc
     9541 aaaaattcga ccaggcaacc cctgtagaat atattgctgt aagagcggca actttatcgt
     9601 ttgcaatgaa acttcttgcg gaaaaaacga ttgttctaga aaccgaatat ttaaaagagg
     9661 agaaaacaaa tgaaaaacaa taaaatcgca attttagtag attcaggtac ggatgttcca
     9721 gaagaattga taaaaaaata cgagatgtat atgatcccat tgaaaattat ttataaagat
     9781 caggtatata cagataaaat tgacattacc cctgaagaga tctatcaacg tttgccagag
     9841 gaaatccctg gtacatcttt gccggatggt gagtcgatca cagaaatctt tgagcaaatc
     9901 aaaaaagacg gttatgaaaa ggtacttgcg gtaacaattt ccagtggatt gagcgggaca
     9961 tataatgtgg ttcgtttaat tgcacaacag caagaagaat tggaagcata tgtactagat
    10021 acgaaaaata ttggtattgg tgcaggtttt tctgcgatcc aagcagcaaa atggctggaa
    10081 gaaggagtag aatgggatca actgatcagt aatctgaatg agttagtcga aagaacaaaa
    10141 gttttcttta atgttgcgac attggaatat cttcaaaaag gtggacggat cggtttagtt
    10201 gcttctattg taggaaccgc tttaaagctt aacccaatca tctcatgtaa tgaagaaggg
    10261 atttattaca cagtaggaaa agctagggga cgtaaaaaaa gtttggataa aacggttgct
    10321 tacgtcaaag aaaaaatcgg taatgcaaag gtcttcaact tagctgttgc ccatggggat
    10381 gcgaaagaag aagcaattga aatggaagcg agacttaaaa gagagttccc acaagcaaac
    10441 caaatctact ttggccagat ttctccggca ttagtagtcc acactggtcc gggattattg
    10501 ggtgtcggtg tacaagtttt agaaggataa atcaaaaaaa ggaaacactg aggtgaaaat
    10561 cagtgttttc cttttttatt gaagttagaa caaaaaataa taaagtattt tgcactttca
    10621 ttttaatttt ctcttttgtt ttataggcaa agatgaaaca aaagcagaaa tacggtataa
    10681 tgagaaggta tgacaataat atataagagg tggctcttta tgaaaaaagt tttagttgtc
    10741 gatgatgaga agccgatttc ggatatcgtc aaattcaatt tagccaaaga aggctatgac
    10801 gtttatactg catatgatgg ggaagaagcc ttggaaaaag ttgctgaggt agaacctgat
    10861 ttgattttac ttgatttgat gctacctaaa atggacggct tagaagttgc tcgtgaggta
    10921 cgcaaaactt atgatatgcc gatcattatg gtaacagcca aagattctga aattgataaa
    10981 gtcctaggct tagagttagg cgccgatgat tacgtgacca agccattttc caaccgagaa
    11041 ttagttgcac gtgtcaaagc aaatcttcgt cgcggtgcca cggctgcgaa ggaaccagaa
    11101 gaggcagcac ctgcggaatt gacgattggt gatttgacga tccatccaga agcttacatg
    11161 gtgacgaaac gaggagaaac aatcgagtta acacatcgtg aattcgaact actcttttac
    11221 ttggcgaagc accttggaca agtgatgaca agagaacatt tattacaaac ggtttggggt
    11281 tatgattatt ttggagatgt acgaacagtg gatgtaactg tacgtcgttt gcgtgaaaag
    11341 atcgaagata atccaagcca tccaaattat ttagtgacac gtcgtggtgt cggttactat
    11401 ttgcgtaacc cagaacagga gtaattggat gaaaagaaaa gtccgctttt ttcgatcagt
    11461 caattttaag atcgctatta cctttattct gattttattg atctcgatcg aaattattgg
    11521 tgcctacttt atacgtggtt tagaacgttc cacgatcaat acatttatta aagatatgaa
    11581 tcaaacggtg gaatcattag caacaacaat cagcccggaa ttgaatcgaa aagataatgc
    11641 agatgatgaa gaggtaaatg cgaatatcaa aagatttatt gaaaacagtg cgactagcga
    11701 tatcatcgaa atacgggtcg ttgacgaaaa agggatcatc cgaggaacaa ccgatgtcaa
    11761 tgaacaaagc gctgtgggaa agaaaaatga ttatgttgat atcaatgatt tcacgactaa
    11821 acgttatgtt gctttagata acgacgataa acgggtgaat atcaatgttc aaccgatcct
    11881 gtccccgacc ggcgatgccg ttatcggggc tctttacgtc aaaagtaata tcgaacaaaa
    11941 atattcggaa atcaataaca cagcagtcat tttctttact gcctcactaa ttgcggcatt
    12001 tatttcaatg atcgtatctg ttttagttgc tcgttcgatt acacagccga tcggtgaaat
    12061 gagagaacaa gccattcgaa tcgctagagg agactacagt cggaaagtca aagtgcatgg
    12121 acaagatgaa cttggacagc tggctgatac cttcaatcag ttagctgaac gtattgaaga
    12181 agctcaagat acaatggaag ctgaacgcaa tcgtttggat agcgtgctat cccatatgac
    12241 agacggcgtc attgcgacag atcgcagggg taaagtcatt acgatcaatg atatggctgt
    12301 ttccttgcta gatatcaaaa acgaggaagc aatcggacaa tcgattttga cacttttaga
    12361 tatcgaagaa gaatatacgt tgcggaaatt attagaagat ccaaacgaaa tggtacttga
    12421 ccgttcaacc agtacattgg aaagtgatca gatgatcttg cgagtagact tttctatgat
    12481 ccgtcgcgaa tcaggtttta tcagcggatt agtagctgtc ttgcatgatg tgacagaaca
    12541 agaaaaaaca gaacgggaac gtcgtgaatt cgtctctaat gtttcccatg aacttcgtac
    12601 accgttgaca agtatgcgta gttatatcga agcattgagc gaaggtgcgt ggaaagacga
    12661 ggaagtagca ccaaattttt tgaaggtaac gctagatgaa acagaccgaa tgattcgaat
    12721 gatcaatgac ctcttaaatc tatctcgcat ggatacaggt aatacccagc ttcaattaga
    12781 atatgtcaat ttcaacgagt tagtgaattt tgttcttgac cggtttgata tgatggtggg
    12841 aaatcaggaa aaaaattata agatccgtcg tgaatttacg caaagagatc tttgggtcga
    12901 agtagataca gataaaatca tccaagtagt cgataatatc atgaataacg cgatcaagta
    12961 ttcaccagat gggggaacga ttacttgtcg cttattagaa acgcataata acgtgatttt
    13021 aagtatcacc gatcaaggat tggggattcc taaaaaagat ctgaaccgag tgttcgagcg
    13081 gttctatcga gtagataaag cacgtgcaag agcacaaggt gggacaggac ttggattagc
    13141 tatttctaga gaagtgatta aggcgcatcg aggcgctatt tgggcagaga gcaaggaagg
    13201 aaaagggtcg actttttata tctcgctgcc atatgaacct tatgaggagg aatggtggga
    13261 atgagaatct ctgaaaaagt cgttcgtata ggactcattt tgatggttgc gttaagcata
    13321 tacttctctt atgcaatctg gctgagccca gcaggaaaaa gttcaatcaa cgtagatgag
    13381 acaaacactc aaatgatcga gtctcaaagc aatcgaaaaa cttccgaagt ctttcttccg
    13441 cttcatgtta catggcacca ttcgggaatg atccaagaaa ccaacagtga aaatctcgtg
    13501 taccgattgc aattgatcat cgataaagca agatttggaa agctttctga agtagtttca
    13561 ggagacaaat cacagtttga acaggtgaaa aatctgaact ctggttttga gttggcctat
    13621 aatgcgcctt ttttgctaag agaatacaag gaagcatttg atttagattt agatgtgtcg
    13681 aacttggcaa aagaaagcga aaatatgtat tttactagaa tacaattcga tcaaggaaat
    13741 aaaaaaatcc gatttttgaa ttattcgaac catctcgtat atgaagcaaa tatttcgttg
    13801 aattggacag atatcaataa agaattaaca gcaaaagaca caaaatggac agaaatgaca
    13861 acagaaccat cgacgatcga cacgcaatat gctacgaaac attcgatcaa actgaagaag
    13921 tatagttata tcctgtcaca gcaaccctat acacttttta gaaatgcttt tttccagaaa
    13981 ccagatagcg tgaaaaataa tgatgaaagc aatgaattga ttttttacgg tgggaatgaa
    14041 acaatgtcta tccattcgga aacgcaagta gttgattttc gtggagagct gccagagaaa
    14101 aaagaaaatg ctagtgtatt ttctgaaagt ttcccctata ttagcaaatt aggcagttct
    14161 ttagggaacc tcagatattt tgaccgcagt gacaatacga tcgattatcg gatattcgtg
    14221 gaaggcttcc cggtttttgg gacagacagc agaggacaga ttggtgtgga agttggcggt
    14281 gaaacgaatg gttctgtgca agtgaatatc caaaccagtt tgaatacgat tcaagtgccg
    14341 ataccttcag acgaagaagt agaacttgca agtacgaatg aaatcatcca gcagttaaaa
    14401 gctaagggag cagatagcaa caagattggt tcagtgatcg ttggctatac atggcaaaat
    14461 gtcaaagaaa cgaatcgtgt cgttgatctc ttacctgaat ggtatgtgaa atataaagat
    14521 aactggtatt cagctaacga ccttctggaa aaattacctg gattggaggc gaattaattg
    14581 gatttcaaaa aaatcgaatg gatatttttc gtcgcttttt tagggttgaa tgtgttttta
    14641 ttcagcagtt atcatgacgc ggtataccaa gaaaacaatg tcattcgctc taatcagact
    14701 gaatcaatcc aacaacgtct ggctagcgac gatatcactt acacggggaa gatttcgtct
    14761 gagaacaagc aaggctatta tcttagcgcg gagcagacca atcttagcac ttcattagcg
    14821 aattatcgca aggctggtaa aagcggcttg ctggcaagtg gtgtgttttt agacggtgat
    14881 gttttgacga aatctttatc cagttcagat gctgaaaaga acgtgatcga tccagataaa
    14941 attagtagtt ccttggaaga ttttttatct aacaaagaca atattttatt cggtaatgat
    15001 tatgtgtaca cgaaaaactt ttctcattta gaagaagaac cactagaaat ccaagcaagc
    15061 caagagtatg aaggcatccc attcaatgat gatacagcaa aaatcgttct ttctttagaa
    15121 aaaaacgata atgattatag catcacgaaa tacatgcaga cgcatttatc tgatatcgag
    15181 cagctgcgcg aaaagacgga actgtatacg gaagaggaag caatcaatac attgtacatc
    15241 aataacaaaa taccaagagg atcaaaaatt ttatggcgtc agctagctta ttcctgtatt
    15301 ctgaaagtcc gggaaaaaaa tgtgtatgtt cctgtttggc atgtagcgat cgaaacaagc
    15361 gataaagtgg tccaaattga gagcgtcaac gctttcagca atacgatcgt gacaaacaat
    15421 actgtaccaa aagtggaaga tcaataaaaa agcgagtata atgaaaaaag tttacaaagt
    15481 aagaagggaa gtaggagtca aatggctgaa gaaaaaccag cttttaaaat cagtatccta
    15541 gcaagtggaa gttcgggtaa ttcactttat atcgaaagtg aaaaaaaacg gcttttggtc
    15601 gatgcaggac ttagcggtaa aaaaatcact tccttgatgg ctcaaattga tcggacaccg
    15661 gaccaattag atggaatact tgtgacgcat gaacatcgag atcatataca tggcgtgggt
    15721 gtcttggctc gtaagtatca tttggatgtt tttgccaatc agcagacttg ggaagcgatg
    15781 gaaccactga ttggaaatgt accagtggaa caaaaacatc tatttgagat ggggaaagtc
    15841 agaacattcg gcgatatcga tattgagagt ttcggcgtct cccatgacgc agcggctccg
    15901 caattttatc gtttccataa agaaaatcgg tcattcgtca tgttgacgga caccggatat
    15961 tgcagtgatt atatgcgagg tgtcattgaa aatgccgatg gttatttgat ggaaagtaat
    16021 catgatcttg agatgttacg catgggacct tatccgtgga atttaaaaca gcgtatttta
    16081 ggagatagag gacatctttc caacgaagac ggtgcacttg tcatgactga tgtgatcggt
    16141 aatcggacca aacgaatcta tcttggccat cttagcaagg aaaacaatat gaaagaactt
    16201 gctcatttaa caatggaaaa tgttttgaaa gaaaaagatt ttggcgttgg acatgatttt
    16261 gaaatctatg atacagatcc agatgaagca actgagctat tttcgattta aacagttcaa
    16321 tcaataaaaa tataaaaagc agaagataaa atcaatcagg gttggtttta tcttctattt
    16381 ttattttttt agcatattaa aaaaataacc gtattatcga ctgactacac tgatgtgctg
    16441 cttttcatgt ttctcatcca gtatctcatc tactaatgca atcgcgtaat ctgccatgct
    16501 gatcgtactg tcgctagcag cgtttctttg taaataatca tcacttaaca aataagtacc
    16561 tgtacgtttg ccatgtggat caaataaaga agctggactg atataagtcc aatgtagatt
    16621 tttagattgt ttcaatttta acaatccttc gtacatattg aaagctgttg gatagaaggg
    16681 tgcattttta ggtacttggt caatcatacg agtcgtcttt tctgtatcta agaataacga
    16741 agaggcaccc ccaacgacaa ttaagcgtgt atcggttcct tcaagcatct caatcaaatg
    16801 ggacaaagaa gtttgatgca tttcttcttt tcctttggga gcgttgaaag catcaacgac
    16861 aacatcaaaa gatgcaagat cagtagttgt caactcaaat aaatcctttt gtataaacgg
    16921 aacatctgat ggcaattttt ctggatgacg cacgattgcc gtcacgtcta aatctctgga
    16981 caatgcttcg tctaaaataa atgaacctgc atggccagta gctccaatga gtgcaatttt
    17041 cataaaaaac ctcctttttt attcctgtcc attgtaagca gttgctgaat attcgtcaaa
    17101 gaatccgatt tttgaaaagc tcagtgcgca ttcctttcat ggttcgtgat cattgggtat
    17161 agttaaatta agaaaactga ttgataaaaa tagaagttga aggagataag aaatgaaaga
    17221 gtggttagat cgtgcctttt taccaatgat aacatctgtt gtgaatctta gacccttaac
    17281 tgctttgcgt aatggaatga cttacacgat cccaataatt attgttggct cgctgttttt
    17341 cttatttgct cattttcctg tggattacat cgctcagtgg atagacaaaa caggattttc
    17401 tatgtatttt acacaagtat accaggctac atttgatgtg atagcctttt ggatcgtttt
    17461 tgccattgca tacgcttatg caaaagaaga agacatcgat cgcttaccag tcggcataac
    17521 agccttgtct gcttatctac tgcttattca gccactaaat actcagatag atactgtctg
    17581 gacaggtgct gaagggatac taggagcagt tacaactggg ctcatcatcg gatggggcta
    17641 tgcacgttta gtaagacttg cgaaagggtg gaagcaaaga gagaagatac ctgaaaatgt
    17701 ttggcagtct tttacttcat tgattccaat cattgttata gtcactttta cgcttatcgg
    17761 ttcagcggtt tttcaacatt tgttcagaat gactgttgtt caaggagtat ggacatatgt
    17821 ccagcttccc attcaatcgt tcatagacac gttgtttggt gtgcttttat taggtttttt
    17881 tgttccgttt ttatggttat ttggtcttca tggatctgcg atggtcaatg ggttggttag
    17941 tcccgtcttg caagcgaatt cattagcaaa tgcagaaatc ttagcctcag gacaagagtt
    18001 gacggtggca aatggcggac atatcgttac tcagcaattt ctcgatcagt tcatgacggt
    18061 tacgggtgct ggattgacgt taggggctgt attttttatg acggtttttg cgaaatcgaa
    18121 gaaatacaga gaattaggaa atcttgctct tttaccagcc tttttcaaca tcaatgaatc
    18181 aattattttt tccacaccga tcgtgatgaa cccgatgatg gctgttcctt ttattttcgc
    18241 accggtcgtt tcaggattga ttacctattc cgctttatat tttggattcg ttcctttatt
    18301 tacagcagtc caagtccctt ggacaacacc gccgatcctc tcgggtttct tagtaggcgg
    18361 agtgcctgca gcgattttac aaggtatcgt gttgtcaatt agttttttca tttatttccc
    18421 atttttgaaa aaaatagatt ctgcaaactg caaaaaggaa agacagacaa aaaatgatgg
    18481 agcagcagaa aagatgaacg actaaaaaac aaaaatactc ctgtcgcttt agattgggaa
    18541 aatcaccaat ctaggatgac aggagtattt atagtcatgt ttatactagc cctttttgga
    18601 cagctaattg aacaagaaca actaatgtgt tcattcctac gtgtgtgatc atcgatgtcc
    18661 agatccgacc agttgctttg tactctaggc tgaagaagaa acctaggaaa aagtagatta
    18721 gcagatgacc atcattatga gcaaacgcaa acagcaaaga gctgatcact gctccgaccc
    18781 aaaaatttga atatcgactg atgatcccca gtatactgcg gcggaatacg aactcttcca
    18841 tgatgggtcc agcaacagtt gtcgccaaaa taaaagcggg tgcttccatg atcatctgga
    18901 tgatattttg tgtattctcg gaaggggttg ctttgccaaa caatacagat tctaacatga
    18961 cagcgatgct ttgcagaatc aatgcgacaa agattccaac caatccataa ataacaatct
    19021 ttggagcaga agcttttttt accgctgatt cgatcgctaa gggttcacgt tgcttaaaat
    19081 aaaaaatagc taggacaacg gcgcctagta tatacatgac tgcagttgct gtgatcaatt
    19141 cagacgtaga ttttacaagt cctatcgaag ctaggatcaa gggtgagata aaaattagac
    19201 catagcaaaa aatgctgagg aagctatatt tttttaaaga catgtaattc ctccttttaa
    19261 gggataacaa gcatgcaagg caagcttata tcgattgatt tcaattatat cataatttca
    19321 ataagcagct gtcagaataa aatttctttt ctttgattta aaagcttgca aaaaagaggg
    19381 agaatgagta ttattaaaaa tgtaggttag cactcgaagg ttaagagtgc taacagatta
    19441 aaaaaagagt tatcggaggg atttatcgtg ttaaaaccat taggtgatcg cgtcattatc
    19501 gaagtcgcaa aagaagaaga aaaaactgtc ggaggaatcg ttttagcttc tgctgctaaa
    19561 gagaaacccc aaactgggac agttgttgca gtcggtgaag gccgtttgtt agaaaacggt
    19621 gaaaaagttc ctgccgctgt aaaagcaggc gaccaagtta tgttcgaaaa atacgccggt
    19681 acagaagtaa aatacgaagg aaaagaatat ctgatcgtcg caggaaaaga tatcatggcg
    19741 attgttgagt aaatttggaa gataaaaaaa taatcaaaaa taatcaatga ggtgaatcaa
    19801 tcatggcaaa agaattgaaa tttgcagaag acgcacgcgc agctatgtta cgcggggtag
    19861 ataaattagc agatacagtg aaagtaacat taggacctaa aggccgaaat gtcgtactag
    19921 aaaaatctta cggctctcca ttgatcacaa atgatggtgt gacgatcgca aaagaaatcg
    19981 aattagaaga tcattttgaa aacatgggtg caaaacttgt atcagaagta gcatcaaaaa
    20041 caaatgatat tgcaggagac ggtacaacga cagcaactgt tctgacacaa gcaatcgttc
    20101 gcgaaggttt aaaaaacgta actgcaggtg ctaatcctct aggcattcgt cgcgggatcg
    20161 aattggcaac aaaagcagca gtcgaagaat tgcataatat ctcaactgtt gttgattcaa
    20221 aagaagccat tgcacaagta gcagctgttt cttcgggttc tgataaagtt ggacatttga
    20281 ttgctgatgc aatggaaaaa gttggaaacg acggtgtcat cacaatcgaa gaatcaaaag
    20341 ggatcgaaac agaactagat gttgtagaag gtatgcagtt tgatcgtggt tacttatcac
    20401 aatatatggt aacagataac gacaaaatgg aagctgttct agaaaaccca tatatcttga
    20461 tcacagacaa aaaaatctct aacatccaag atattctgcc attgttagaa caaatcttgc
    20521 aacaatcacg tccattgttg attattgctg acgatgtaga tggtgaagca ttgccaactc
    20581 ttgtattgaa caaaatccgt ggaacattca acgtagtagc tgtaaaagct cctggtttcg
    20641 gcgatcgtcg taaagcaatg cttgaagata tcgctatttt gacaggtggt acagtaatta
    20701 ctgacgatct aggtttagaa ttgaaagatg caacaatcga aaacttagga aatgcttcta
    20761 aagttgttgt agataaagac aatacaacga tcgtagaagg ttctggagaa aaagaagcaa
    20821 tcgaagctcg tgtgcaattg atcaaaaacc aaattgctga aacaacttct gattttgacc
    20881 gcgaaaaatt acaagaacgc ttagctaaat tagcaggtgg tgtagctgtt gttaaagttg
    20941 gtgcagctac tgaaacagaa ttgaaagaat taaaattacg cattgaagat gcattgaatg
    21001 ctacacgtgc cgcagtagaa gaaggaatgg tctcaggtgg tggtacagct ctagtgaacg
    21061 ttatcagcaa agtttctgct gtagaagcag aaggagacgt ggcaacaggt atcaagatcg
    21121 ttgttcgtgc tttagaagaa ccaattcgtc aaatcgctga aaacgctggt tatgaaggat
    21181 cagtgattgt tgataaattg aagaacgtgg aactaggcac tggtttcaat gctgctactg
    21241 gagaatgggt aaacatggtt gaagcaggta tcgtagaccc aactaaagta acacgttctg
    21301 ccttgcaaaa tgccgcttcc gtttctgcct tgttattaac gacagaagca gtcgttgcag
    21361 ataaaccaga accagcagcg ccagcagctc ctgcaatgga tccatcaatg atgggcggta
    21421 tgatgtaaga aaaagcaact tgcttatcta caactgatca ttagtgaaag acagataaaa
    21481 agaggctgga acagaagcgt ttaactccaa gaaataagaa gaaattcacg gaaattgctt
    21541 ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt ccgctgttta
    21601 tacgtgttta gagcgtgaga c
//
LOCUS       DAZKOU010000052        20968 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000052 DAZKOU010000000
VERSION     DAZKOU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.053"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACG827_001785"
     CDS             complement(<1..563)
                     /locus_tag="ACG827_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0483924.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACG827_001786"
     CDS             complement(750..1400)
                     /locus_tag="ACG827_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483925.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG827_001787"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG827_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0483926.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG827_001788"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG827_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0483927.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG827_001789"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG827_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0483928.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACG827_001790"
     CDS             complement(4926..5657)
                     /locus_tag="ACG827_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0483929.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACG827_001791"
     CDS             complement(5661..6926)
                     /locus_tag="ACG827_001791"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0483930.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG827_001792"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG827_001792"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0483931.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG827_001793"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG827_001793"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0483932.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACG827_001794"
     CDS             12268..13770
                     /locus_tag="ACG827_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0483933.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACG827_001795"
     CDS             13772..14509
                     /locus_tag="ACG827_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0483934.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="ACG827_001796"
     CDS             complement(14680..15873)
                     /locus_tag="ACG827_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0483935.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="ACG827_001797"
     CDS             complement(15952..16158)
                     /locus_tag="ACG827_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0483936.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="ACG827_001798"
     CDS             complement(16151..17293)
                     /locus_tag="ACG827_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0483937.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="ACG827_001799"
     CDS             17805..18440
                     /locus_tag="ACG827_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0483938.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="ACG827_001800"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="ACG827_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18985..19032
                     /locus_tag="ACG827_001801"
                     /pseudo
     CDS             18985..19032
                     /locus_tag="ACG827_001801"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG827_001802"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG827_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0483939.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         6172 a         4299 c         3722 g         6775 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagt
//
LOCUS       DAZKOU010000053        20407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000053 DAZKOU010000000
VERSION     DAZKOU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.054"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..282
                     /locus_tag="ACG827_001803"
     CDS             <1..282
                     /locus_tag="ACG827_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0483940.1"
                     /translation="ILLLLNIWIFDRSRNASIGFRTKRSLSSKKNWVYSQTIFYGGIV
                     LISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(486..851)
                     /locus_tag="ACG827_001804"
     CDS             complement(486..851)
                     /locus_tag="ACG827_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483941.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1059..1541
                     /locus_tag="ACG827_001805"
     CDS             1059..1541
                     /locus_tag="ACG827_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483942.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1738..2628
                     /locus_tag="ACG827_001806"
     CDS             1738..2628
                     /locus_tag="ACG827_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0483943.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2953..5424
                     /locus_tag="ACG827_001807"
     CDS             2953..5424
                     /locus_tag="ACG827_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0483944.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5527..7263)
                     /locus_tag="ACG827_001808"
     CDS             complement(5527..7263)
                     /locus_tag="ACG827_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0483945.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7260..7964)
                     /locus_tag="ACG827_001809"
     CDS             complement(7260..7964)
                     /locus_tag="ACG827_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0483946.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8095..8598
                     /locus_tag="ACG827_001810"
     CDS             8095..8598
                     /locus_tag="ACG827_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0483947.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8558..8896
                     /locus_tag="ACG827_001811"
     CDS             8558..8896
                     /locus_tag="ACG827_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0483948.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8917..10335
                     /gene="ffh"
                     /locus_tag="ACG827_001812"
     CDS             8917..10335
                     /gene="ffh"
                     /locus_tag="ACG827_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0483949.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10447..11025
                     /locus_tag="ACG827_001813"
     CDS             10447..11025
                     /locus_tag="ACG827_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0483950.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11029..11550
                     /locus_tag="ACG827_001814"
     CDS             11029..11550
                     /locus_tag="ACG827_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0483951.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11629..12183)
                     /locus_tag="ACG827_001815"
     CDS             complement(11629..12183)
                     /locus_tag="ACG827_001815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0483952.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12436..12711
                     /gene="rpsP"
                     /locus_tag="ACG827_001816"
     CDS             12436..12711
                     /gene="rpsP"
                     /locus_tag="ACG827_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0483953.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12723..12974
                     /locus_tag="ACG827_001817"
     CDS             12723..12974
                     /locus_tag="ACG827_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0483954.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13099..13890
                     /locus_tag="ACG827_001818"
     CDS             13099..13890
                     /locus_tag="ACG827_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483955.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14060..14581
                     /gene="rimM"
                     /locus_tag="ACG827_001819"
     CDS             14060..14581
                     /gene="rimM"
                     /locus_tag="ACG827_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0483956.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14571..15332
                     /gene="trmD"
                     /locus_tag="ACG827_001820"
     CDS             14571..15332
                     /gene="trmD"
                     /locus_tag="ACG827_001820"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0483957.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15468..15815
                     /gene="rplS"
                     /locus_tag="ACG827_001821"
     CDS             15468..15815
                     /gene="rplS"
                     /locus_tag="ACG827_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0483958.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(15947..17083)
                     /locus_tag="ACG827_001822"
     CDS             complement(15947..17083)
                     /locus_tag="ACG827_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0483959.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(17199..17765)
                     /locus_tag="ACG827_001823"
     CDS             complement(17199..17765)
                     /locus_tag="ACG827_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFQ0483960.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(17901..18554)
                     /locus_tag="ACG827_001824"
     CDS             complement(17901..18554)
                     /locus_tag="ACG827_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483961.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(18604..19032)
                     /locus_tag="ACG827_001825"
     CDS             complement(18604..19032)
                     /locus_tag="ACG827_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0483962.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(19037..19411)
                     /locus_tag="ACG827_001826"
     CDS             complement(19037..19411)
                     /locus_tag="ACG827_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0483963.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            19715..19900
                     /locus_tag="ACG827_001827"
     CDS             19715..19900
                     /locus_tag="ACG827_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0483964.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            19913..20161
                     /locus_tag="ACG827_001828"
     CDS             19913..20161
                     /locus_tag="ACG827_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483965.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            20192..20362
                     /locus_tag="ACG827_001829"
     CDS             20192..20362
                     /locus_tag="ACG827_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483966.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
BASE COUNT         6623 a         3620 c         4012 g         6152 t
ORIGIN      
        1 atattattac ttctgaatat atggattttt gataggagta gaaatgcaag tataggtttt
       61 agaactaaac gaagtctgtc ttctaaaaaa aattgggtat actcacaaac tattttttat
      121 ggaggaattg ttttaattag tttactttct tcaacacttt attctttgaa tattatagat
      181 gtttccacct caaattcaat atctattatt ggaatcatta ttgctgcaat tattacacaa
      241 ctgtttttag tgtttggaga gaagaaaaga agtaaaaaat agaaaaacta gtgatagttt
      301 tggtgatagt gatattaaaa atcagaaaag atagaaaaag caggagatag ataaaatgct
      361 gatatttcag cattttttaa gagtataaaa gagaaaaaaa gtaataaatg tatacatgat
      421 ataagaaaaa catatatgta taaaaagaag aaagtagctt ctctttgtac atatatggtt
      481 tgtttttatg gatgccgagc ttttttataa gataaggcta ccgcccataa cgacccaagg
      541 atttgtggaa ctaagtaaat acctgcaaga agcaagatca gctgggtcgg ttttaaagga
      601 tgatagaagt aacgccattc agtaaaattg gcgcaaaata aataggaagt gacagtagat
      661 ataatattgg caatgaataa agatataata cgcctgttcc tttgaaaata atatccagca
      721 aaaagagata aaaatatcaa tgtgatgaac ccgcttacgg aatgataacg aaaatcaagc
      781 agtaaggcga aatgcacata aggaacaaaa gataaaagca acggaaggtt tttccagcaa
      841 gtttttatca tctttcatca ccacctaaca agtgttatta ttttgttagt tttattgtaa
      901 attgaatcct gcaataatca attacttttt cattatttct agattaaatg aataaactct
      961 catgccatta tctctaaaaa aaaagttttt ccccctaatt tttttagata gaaaattgta
     1021 tagtatgatg agagaaaaag tagtttgggg gaacgttaat gagaagaaaa gcagtaatcg
     1081 atcggcttta tttatttttt tggttttttc ctatcgtttt gctacctttt acatgggtaa
     1141 atgctgttgt acaaggtgcg gagatattag gcctgatcca aggaagcgga tttgtggttt
     1201 ctaggaattt tccagctttg actgttttct atttagtgat aagtgtaggt ctcttattac
     1261 caaaatctct gatcaatcac tacaattggc agctgcttta cttttcttgc ttattagtat
     1321 ttacatctat gttttctttg ctgcctcgct tgattatcgg ccctaatggt ttgagcgaga
     1381 gtggattgaa ggattcagct tattcctcca tgtatttctt gacgatcaaa ccatggttct
     1441 atttagtaat tgcctcattt attcttgcaa cattttttta cttctggaca gaaatcagga
     1501 taaaaagtga acgatctgta tctataaaaa ataactattg atggtggatc atcagtaaaa
     1561 aagcatatga gctgcgtaaa atgcatgttt ctcatatgct ttttttatta ttatgtaaag
     1621 aatgcgaaaa acgtttacat gatttttaca taacttaact atatttttac agcaaccata
     1681 cgagggattt acagtcgact gataaacttt agttgtaaat aagaaaggag caataaaatg
     1741 aaaaaagtat tgtttactct aggtatgatg ggaattcttt tagccggatg cggctcagga
     1801 tcaggaaata cagatggctc tgccacacaa aataattcaa gtgactcaaa tgaacaagta
     1861 aaaatcgttg cggtaggttc gacagcatta cagccgttag tcgatgcagc acaagaaagc
     1921 tttgtacaag aaaatccaaa ttatcaaatc tctgtacaag gaggaggaag cggaactggt
     1981 ttgagccaag tcgaagctgg agcagttact atcggaaact ctgatgtatt tgcagaagaa
     2041 agagacggag ttgatgcttc aaaattagtg gatcataaag tagcagtggt cgggatggcg
     2101 ccaatcgtca ataaagatac agatgtcaaa gatatcacca aacaggaatt gattgatatc
     2161 tttacaggaa aaatcactaa ctggaaggaa gttggcggga aagatcaaaa aatcaacgtc
     2221 gtgaaccgtg caaacggcag cggtacccgc gcgacattcg aaaaatgggg actggatggt
     2281 gctacccctg tgcagtccca agaacaagat tcatcaggaa cagttcggca gctggtaagt
     2341 caaacaccag gagcaatcag ctatttagct ttctcatatc tcgatgattc cacacaagca
     2401 ctaagtatcg acggtgtaga accaaaagaa gagaatgtag cagataacag ctggggaatc
     2461 tggtcatatg aacatatgta cacgaacgga aaaccttccc ctgaagtcca aaaattctta
     2521 gactacatga tgacagaaga aatccaagaa ggacctgtca aagaattggg ctatttacca
     2581 atcacaatga tggaagtaga acgtgatcac gaaggaaata tcaaataact agcagataaa
     2641 catgccacgg tagtcgttac tattaaggaa aaatcaacta tgtttcccac atatacattt
     2701 ttcctttaat tgtgaaaaat agttcctcat attttgccgt tttttgagct aataccgagg
     2761 gtcagctgat taaacaccgt ttattcgaaa aaaagagcta cgacatagaa ctgtcgtagc
     2821 tctttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt
     2881 tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa cgaaatcaat
     2941 cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa agtaacacaa
     3001 agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt aacggcagat
     3061 caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa
     3121 acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat ctttcttatc
     3181 ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt accagttatc
     3241 atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat cttggacgaa
     3301 ctaaatgttt taaatacagc aaaagtcaat gtcatacggg acgggataga acaaacaata
     3361 gatatagaag accttgtaat agatgacatt gttattttag agactggaca tcaaattcca
     3421 gcagatgcag aggtcgtgga aggaaaactg caagttaatg aggctttatt gacaggcgaa
     3481 gcagatgaaa taaccaaaga aataggcgat ccattaatgt ccggcagttt cattgtttcc
     3541 ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc ccagctgaca
     3601 gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc tcttaatcaa
     3661 ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt ttctcaaagt
     3721 tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc agcattgatc
     3781 ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt aagtgcgact
     3841 aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac attggcaagg
     3901 gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat gtctgttcaa
     3961 aaagtgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt ggaagcatgc
     4021 attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc tgttaaagca
     4081 tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc ttctgtccaa
     4141 aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt
     4201 ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga aaaaggctat
     4261 cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt gacagaagca
     4321 gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc aagagccaca
     4381 tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga taatccgctc
     4441 accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta cgttgatgca
     4501 tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt ttttggacgc
     4561 gtcaagccag aacaaaagaa aatcttcgtt caattgataa agcgtaacaa tacagtagct
     4621 atgacaggtg atggcgtgaa tgatattcta gccatgaaag aagcagactg cagtatagcg
     4681 atggcttccg gaaatgaagc cgccatgcag gcctcacaag tggtcttact tgattctgac
     4741 ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga
     4801 tcagccagct tgtttttagt gaagaatatt ttctcctttt tgctctcact gttttcagtg
     4861 atctttgctc tgacctatcc tttagaacca tcacaaatca cattgattag tttgtttact
     4921 atcggacttc catctttctt gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa
     4981 ttcatcatga atgtgatgga aaaagctgtg ccaggtggac taacagatat gatcgtagtc
     5041 ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata aaacagatgt ttctacagca
     5101 tctaccatgc ttttgattgc agtagggttc ctcgttctct ataaaatctg ttcaccgctg
     5161 aataaattca gaagtcagat cattcttttc tgtgctagcg gtaacttttt ttcggtcatc
     5221 tttttgcata agctgttttc aatcaccggt atctcagctg tatcactttt gctgttgagc
     5281 attctatttt tctcggcaga ttcgattttt aggcatttga ccacattggt agaatatttg
     5341 tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta
     5401 tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac
     5461 cactcgtgta tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc
     5521 tcttttttac attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc
     5581 tacagtaatc gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc
     5641 ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg
     5701 ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc
     5761 tgttgcctga tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc
     5821 aatcagattt ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt
     5881 tccaatcaat cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat
     5941 ttgctggaaa taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt
     6001 tgacagctgg atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat
     6061 ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt
     6121 aaaaccaatc agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc
     6181 cttttgtagc ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg
     6241 tccggtacct tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc
     6301 tgtaagggtg atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg
     6361 aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac
     6421 gtttaaatgt agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat
     6481 caggaatacg ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt
     6541 acacgcccga tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc
     6601 ttcccattga tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt
     6661 ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat
     6721 cattcctgca agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact
     6781 ctcagttctc ggtaaaaaac cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg
     6841 gccacttttt ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt
     6901 gcgcaaagcg ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt
     6961 tcgctgttca ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat
     7021 taaagtaatc cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc
     7081 tgcttggagg ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta
     7141 gctttcttgc tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc
     7201 aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt
     7261 catcgttttg gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttgga
     7321 tgttttggat ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg
     7381 ctttgtcctg cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca
     7441 cggtcttttc gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc
     7501 agatggtcgt ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat
     7561 agatattcag gctcagcttc atcttttgat tgcttacgag gttcaatccg tctgaatatt
     7621 gctttcatac gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca
     7681 cctatttcta ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc
     7741 ggtgtttcat ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga
     7801 agcatcacat ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg
     7861 cctccatctg tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc
     7921 gttacaatag aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa
     7981 taggtttcat tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag
     8041 aaataaatgg tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt
     8101 ggaatcagtg catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta
     8161 ccagaattga tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc
     8221 ggcggattac caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg
     8281 tggaaagatc aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa
     8341 aatggcgcga tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg
     8401 aaggaaaaaa gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata
     8461 aaaaaaaatg gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc
     8521 tctgatgaag aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg
     8581 aatgaacgcg ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat
     8641 ggaattatat tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag
     8701 ccgacaagca gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa
     8761 aaaactccat cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc
     8821 atatattaca gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca
     8881 agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa
     8941 cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta
     9001 aaagaaatga tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta
     9061 gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt
     9121 ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt
     9181 tctgaaacag cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt
     9241 cttcaagggg caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat
     9301 gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa
     9361 ttgaaagtat taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc
     9421 ccggtagaga tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta
     9481 ttgatcgata cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa
     9541 atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa
     9601 gatgccgtca acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc
     9661 actaaacttg atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc
     9721 gcaccaatca aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca
     9781 gatcgtatgg ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg
     9841 cagcaagact acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca
     9901 ttcgatttca atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa
     9961 gatctaatca aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta
    10021 gatccaaaag atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga
    10081 gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac
    10141 agtgttgttg aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag
    10201 caaatgtcca aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc
    10261 aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag
    10321 aaaaaacgga aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg
    10381 gagcttttct atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg
    10441 gcacagatga gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact
    10501 gtctggggtg ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt
    10561 aatcaagccg gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca
    10621 tatgatcgat tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta
    10681 atggaaaaca aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc
    10741 aaagcagtct tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt
    10801 tctaatggaa aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt
    10861 cctctttcag atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc
    10921 acaacaatct atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt
    10981 ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc
    11041 atattttatg gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa
    11101 tggctttttg acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc
    11161 gatacgacca tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa
    11221 gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca
    11281 gatgctgagg tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa
    11341 ggaaaagata tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa
    11401 atgattgatg aatggtatat ccagattacc cctgtattat taggggacgg gattccttta
    11461 ttccaaaaag gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa
    11521 tttatcgaat tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat
    11581 gcagaaatgt cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa
    11641 tttgttagaa aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc
    11701 aacgatatta ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg
    11761 ctgcaaggtt ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa
    11821 gccgccgatc ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact
    11881 gtgtccataa ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg
    11941 gttgtattca ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg
    12001 agtccattca gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc
    12061 atcgattttt acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc
    12121 tacttttttg ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt
    12181 catacgattt ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga
    12241 gcaaataaaa acacttactg acaaaagcta catagaagat agattactta tttttaacaa
    12301 aaaagaagtc taaaatcttc tattttctaa aaaaaatcga atttaatcaa gctgtaaaga
    12361 aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg
    12421 aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt
    12481 ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa
    12541 actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta
    12601 gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca
    12661 aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg
    12721 atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg
    12781 atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg
    12841 ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca
    12901 caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg
    12961 ataaggaaaa gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt
    13021 tttattaatt gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga
    13081 ggagggagtg aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta
    13141 agcgcttgta gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct
    13201 gaaactgtta ccagtccaga aaaaagtagt accaaaacgg cttccaccac tgaacaaaca
    13261 atggctacct caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca
    13321 aatgttccca tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca
    13381 catacaaagc aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat
    13441 gctgacgaat tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct
    13501 tctagtcaag aaacaatcca agttttgcag ccatttgaga tcgacacaaa tggtcagcaa
    13561 gtggacttag gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt
    13621 gaatggcaag aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat
    13681 cccttactat tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct
    13741 gaacaatttg gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta
    13801 agctggcaag aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg
    13861 aagatggcag tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt
    13921 aactccaagt cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct
    13981 taatacctta aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa
    14041 aaagaaatgg ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca
    14101 aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa
    14161 aaaaggaacg gtgctaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa
    14221 aagtcatcgc aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa
    14281 tgatgtggaa aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct
    14341 agctgaaaat gaattctatt accatgagat catcggtctg catgtattag aagatggaac
    14401 tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca
    14461 acgtccgaaa aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga
    14521 tttagaagct ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg
    14581 atgtattgac tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta
    14641 aggctgtaga taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata
    14701 acaaacatca gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag
    14761 tccagcccat ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa
    14821 gagtaattct tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt
    14881 cacaagaaga acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc
    14941 gcacattagt gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag
    15001 gtgcgatggt catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga
    15061 cttcagctca aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc
    15121 cggcagaata taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga
    15181 ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt
    15241 tggaaacact tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag
    15301 aacaagaaaa aacagcagat gaagccaagt gactttttct aaaatcttct ttacagtagg
    15361 ttgataatat gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg
    15421 ctgtgagaaa agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa
    15481 tcgaagaatt aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca
    15541 ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag
    15601 gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt
    15661 ctaacggagt gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg
    15721 aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg
    15781 gaaaagctgc tcgtatcaaa gaaatccgtc gttaatcatc gttatcagac agagaaagaa
    15841 cccttggtat tcaagggttc tttttttatt atctgttttt ttatattaac gatttctatt
    15901 tggtacattt ttgattaata aaaaggggca aaaaaggggc aacaagttac agtttttcta
    15961 tatcttcttt caatttttct tttactcctt ttgtcacatg caaataaatt ttttcagtga
    16021 tatcactatt ttcgtgacca acgcgatcct gaatggcata cagaggagtt cctagttccg
    16081 ctaattttga aatatgggta tgtctaaaaa tatgtgaact aagtttttta tcaatcttca
    16141 tgtcagcttt atgacttctc aaataggtgt taattgctgt tagttggaaa ggagttcctt
    16201 tcgttgtttg aaatagaaat tgaccatttg gatttaattc tagaagctca ttgtaaatag
    16261 ctatcgcttt ttttggtaaa tcaatttctc gcattccggc agctgttttt gtagaatcag
    16321 attttttcat atcagctatt gaacgttctc tatacatcat cgtcccattt ataactactg
    16381 atgcattatt attggtaatg tgtacatcat ctttacttaa tgcgattgct tcaccagacc
    16441 tcatcccagt caaatatagc cactggaaaa ggagagaata tcttttattg tgtgaagttg
    16501 tgaaatctac tagtctatta tattcatcat cttctaaaaa tttatcttta atttttatcg
    16561 tttttgactc tcttttataa tcaataacta cttcatcgat aggatttttt tctacataac
    16621 cttttttcat agcatatgaa aaaagaagat tcaatttgga tttaattaca ctgacgtact
    16681 tatttgacaa gtcatcttta tatatcatgt cttcaaaaat attattcaaa tctattgtat
    16741 taataccaga aacgatgtaa gtctcaggta tcttcttctt tatagtgttt aatatattat
    16801 tcgtaggata atatgtagat tccttaactt gtcttttgta tataactaac cattcttcaa
    16861 caagctcatg aaatgttaga tcaggcttat gaagtgtttt ttcgtttaat tttatatcaa
    16921 tttttttatt taactctagc aatgccactt tctgagtttc tcgagattta tttttataag
    16981 taatgctcac tttttttctt tttctagttt ttggatcaat atatcgctca ttatatttat
    17041 atactttttt tccgttttta tcagttttag tttcgatcca cattgttttc atctcctatt
    17101 tgttgctata ataggaatag ataagtaagc cgattatagc aggtttattt ttcatcacgt
    17161 ccacaaactt tggcgaggga ggggcgtgtt tttgttagtt attgtggtag gttatctaaa
    17221 gcgtattgtg cttgctcatt tgtaaaacct tcaaatataa gttgatcgta caattgcgaa
    17281 tctgacattg atgcccaatc atcgtaactt tccgctttag ccaaagcttg ttcgttgtaa
    17341 tcgacatcaa cattagccag cgcataatcg attgcatcac ttggatattc ttcaaactct
    17401 agttgttcac gcaaaccttg ttcagacata ccagcccaac ctagatagct ttcagctttg
    17461 ctcaatgcat ttctgtattc acgaggaaca ctttcttctt gagtaacttc ttctatagta
    17521 gtttcagtag tttcagtttc ttcttcaacg actgtagatt ctgctaccga ttctgcagaa
    17581 gacacttctg tcacgctttc tttagttgtt tcagcgacgg tatttttagc atcatcactg
    17641 cctccttgcg aacctatagc aataaacacc acaacaaccg ctaacaacca aaaccatacc
    17701 cgtttataaa acggtttctt aactttatac attttcccat cttgacccat aacttttttt
    17761 gccatttaaa tatccctcgc ttcttgttat aatatatttg tgatctcaga aatgaggtat
    17821 gagtccgtgt tgcagcacgg gcttttttct ttataacttt tttagagatt ataggcaaaa
    17881 tagtagggca taaaaatgta ttattgaatt ccgtatttag aaaatcctaa ttgaacttca
    17941 ccggaagtct tttgctgtgt tgtacgcaat gcttcttctt cagacattcc attctgtact
    18001 ttccatgcaa caggcgacat cccgtatttg ttaacaaaat cagtaagtga taaagtgtca
    18061 gcgtcttgct gagcgcttgt ttgttggtct tctggattct gttgagatgc tgcttgttgt
    18121 tctttctgat cttgactgat aatattgcca gcatcatctg tagtcaatcc attttcataa
    18181 agggccacgc cgaaagcttc ccactctttg ttggaccaat ttgcacgatc agctggagtt
    18241 gactgtaaag tgcgttgttt catctgttca tatgtttctt cttgaggtgc ggtttggatt
    18301 gtactctgac tggagcttat aactgttggg ctaggttccg ccgtagcttg gttggagctt
    18361 gtaactgttg aactagaatc tgttttagat gtagatttgc tagtagatga actggtttca
    18421 gttgtttctt ttgttttact tatttttgtt tcttggttag aagtggtatc tgttgattca
    18481 gcttttttat tatttgaaca agctgaaagt agcagagcag tacttaacaa caacataacg
    18541 ctaacttttt tcattttata attcctcttt ctcgttgtaa tatgtgtgct aacacgggct
    18601 tttttatata agaaaacgat aagcgctttc tggaagcccg taaagattct ttaattcctc
    18661 gattttttta ggatattgat cattatcttc tttataaaga gaaacaatga gattagcagc
    18721 aaagcaatta gcttcgcttt cagatttgct tctagatgtt cttgttgata cataatagct
    18781 ggataagcca cgatgaaaaa tagcgtgacc taattcgtga gcgcaaatgt agaatctttc
    18841 ctcagagtct cgcagttcat catttaaaaa gattatcgca cgatctctaa tttcttgaaa
    18901 ctgccccttg ggattctcga taaaaggaac gtattgaatt ttaatgccca tcttttcaca
    18961 aatataaaaa ggattagcgg actggtattt ccgcttcaac tcctcgacta aattaatcgt
    19021 atccatctcc ataagctcac atctttttgc ctttttcttt gtcttctttc acaatatccc
    19081 agaaagtcgc tatcaggata tcttttacgc gctgtatttg ttcgggtgtc aatgtttccc
    19141 caccataaga catattaaca tttgagtcta gtagtttatc aagttcaacc acttcctctt
    19201 ttgtagccca cttgggaaca ttattatttc ccaataaata atcagttgtg acaccaaaat
    19261 aatcagcaac tttctttaag ttctcagatt ttggcgaggc tttatcccat cttcttattt
    19321 gtccattaga gatgcccacc tgtctttcta cttctgctat agtcacatgc ttttcgtctg
    19381 ctaattcttt aatcttagta actaaactca ttattatcaa cctttcaaag ctgaaaagaa
    19441 aataaatagc ttaaaagtta tatttttggt tgacaattag cttttaagct agtatattta
    19501 attcgtaagc taaattgtta gctaaataag agcaacaaaa aactctacta atttaaaaca
    19561 ttctctcggt cgccaaactt agaaatgtta ttttagaggc tttttataag tcttatttaa
    19621 ctatgtattc ataatagctt aaaagctaat aggtgtcaac gatttagcta attttttagc
    19681 ttacaaatta tttgtttaga aaggagctat ttttatgtct gagaatttag acttaaaaat
    19741 tcgagcggag atgagaaaaa gaagaatgac tttcaaagaa ctagctgcgc ttatcggtat
    19801 ttcaggagct tatttatcag atattctaaa cggcaatcgt gatggaaaga aagcacaaca
    19861 gcatatcgaa acagtgaaaa aaatattgga catccgatag ggggggtagg cgatgacaaa
    19921 actaaaaaaa caagattttg taaaaaaata taattattct ccatctactt atcaacgtcg
    19981 aatgtcggaa ctaaaaaata cagcaatttt ctcagcggcg tatgaacggg tcacaggaca
    20041 agaagtttgg atcaatacag aattatacga taaatttttg tctttcaaat cctataacag
    20101 gttacgcaca agaaaggtaa cacctaaaga atttatcgag aagcatttag ttgatttata
    20161 aaaaacagaa cattttgaga ggtgaaggtt aatgggtaaa ttcaacagag cattagtatt
    20221 cagcgcaccg ctaatcgtct atgctttagg gctttgggga agtagacaag cgttgatagg
    20281 aacgattgtg tacatggttt ggatttttat agggctggat gaagctgagt acaaaacaaa
    20341 aaagccagtc gggagggact gactaatgag taaggacgat attgtatttc tgttaattat
    20401 gttttgt
//
LOCUS       DAZKOU010000054        20360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000054 DAZKOU010000000
VERSION     DAZKOU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.055"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACG827_001830"
     CDS             291..1430
                     /locus_tag="ACG827_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0483967.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACG827_001831"
     CDS             complement(1497..2072)
                     /locus_tag="ACG827_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0483968.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACG827_001832"
     CDS             complement(2077..3051)
                     /locus_tag="ACG827_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0483969.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACG827_001833"
     CDS             3334..4302
                     /locus_tag="ACG827_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0483970.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACG827_001834"
     CDS             4415..4693
                     /locus_tag="ACG827_001834"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0483971.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACG827_001835"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACG827_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0483972.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACG827_001836"
     CDS             5150..5905
                     /locus_tag="ACG827_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0483973.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACG827_001837"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACG827_001837"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0483974.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACG827_001838"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACG827_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0483975.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACG827_001839"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACG827_001839"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0483976.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACG827_001840"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACG827_001840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0483977.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACG827_001841"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACG827_001841"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0483978.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACG827_001842"
     CDS             10642..11184
                     /locus_tag="ACG827_001842"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0483979.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACG827_001843"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACG827_001843"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0483980.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACG827_001844"
     CDS             12777..13679
                     /locus_tag="ACG827_001844"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0483981.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACG827_001845"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACG827_001845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0483982.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACG827_001846"
     CDS             15122..15544
                     /locus_tag="ACG827_001846"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0483983.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACG827_001847"
     CDS             16013..16243
                     /locus_tag="ACG827_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0483984.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACG827_001848"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACG827_001848"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0483985.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACG827_001849"
     CDS             17654..17830
                     /locus_tag="ACG827_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0483986.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACG827_001850"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACG827_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0483987.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACG827_001851"
     CDS             18325..18708
                     /locus_tag="ACG827_001851"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0483988.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACG827_001852"
     CDS             18877..19761
                     /locus_tag="ACG827_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0483989.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACG827_001853"
     CDS             19875..20111
                     /locus_tag="ACG827_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0483990.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="ACG827_001854"
     tRNA            20199..20279
                     /locus_tag="ACG827_001854"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="ACG827_001855"
     tRNA            20286..20357
                     /locus_tag="ACG827_001855"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DAZKOU010000055        19694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000055 DAZKOU010000000
VERSION     DAZKOU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.056"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACG827_001856"
     CDS             complement(<1..210)
                     /locus_tag="ACG827_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0483991.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="ACG827_001857"
     CDS             complement(216..446)
                     /locus_tag="ACG827_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483992.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="ACG827_001858"
     CDS             1063..1725
                     /locus_tag="ACG827_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0483993.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="ACG827_001859"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="ACG827_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0483994.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="ACG827_001860"
     CDS             5145..5459
                     /locus_tag="ACG827_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0483995.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="ACG827_001861"
     CDS             5472..5846
                     /locus_tag="ACG827_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0483996.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="ACG827_001862"
     CDS             5856..6200
                     /locus_tag="ACG827_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0483997.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="ACG827_001863"
     CDS             6271..7623
                     /locus_tag="ACG827_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0483998.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="ACG827_001864"
     CDS             7683..8825
                     /locus_tag="ACG827_001864"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0483999.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="ACG827_001865"
     CDS             8850..9104
                     /locus_tag="ACG827_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484000.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /gene="mobT"
                     /locus_tag="ACG827_001866"
     CDS             9360..10544
                     /gene="mobT"
                     /locus_tag="ACG827_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0484001.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="ACG827_001867"
     CDS             10541..10678
                     /locus_tag="ACG827_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0484002.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="ACG827_001868"
     CDS             10683..10904
                     /locus_tag="ACG827_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484003.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="ACG827_001869"
     CDS             10917..11417
                     /locus_tag="ACG827_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0484004.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="ACG827_001870"
     CDS             11514..13361
                     /locus_tag="ACG827_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0484005.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="ACG827_001871"
     CDS             13364..14260
                     /locus_tag="ACG827_001871"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0484006.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="ACG827_001872"
     CDS             14310..14699
                     /locus_tag="ACG827_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0484007.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16185
                     /locus_tag="ACG827_001873"
                     /pseudo
     CDS             14686..16185
                     /locus_tag="ACG827_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG827_001874"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG827_001874"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0484008.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18872..19694
                     /locus_tag="ACG827_001875"
                     /pseudo
     CDS             18872..19694
                     /locus_tag="ACG827_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         6655 a         3219 c         4308 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DAZKOU010000056        19614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000056 DAZKOU010000000
VERSION     DAZKOU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.057"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG827_001876"
     CDS             complement(<1..132)
                     /locus_tag="ACG827_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0484009.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..781
                     /locus_tag="ACG827_001877"
     CDS             542..781
                     /locus_tag="ACG827_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484010.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            848..1057
                     /locus_tag="ACG827_001878"
     CDS             848..1057
                     /locus_tag="ACG827_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484011.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            1117..1788
                     /locus_tag="ACG827_001879"
     CDS             1117..1788
                     /locus_tag="ACG827_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0484012.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1841..3817
                     /locus_tag="ACG827_001880"
     CDS             1841..3817
                     /locus_tag="ACG827_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0484013.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3830..4585
                     /locus_tag="ACG827_001881"
     CDS             3830..4585
                     /locus_tag="ACG827_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0484014.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4582..5406
                     /locus_tag="ACG827_001882"
     CDS             4582..5406
                     /locus_tag="ACG827_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0484015.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5416..5664
                     /locus_tag="ACG827_001883"
     CDS             5416..5664
                     /locus_tag="ACG827_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484016.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5707..7806
                     /locus_tag="ACG827_001884"
     CDS             5707..7806
                     /locus_tag="ACG827_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0484017.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7844..9901)
                     /locus_tag="ACG827_001885"
     CDS             complement(7844..9901)
                     /locus_tag="ACG827_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFQ0484018.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(10015..10227)
                     /locus_tag="ACG827_001886"
     CDS             complement(10015..10227)
                     /locus_tag="ACG827_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484019.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            10414..10881
                     /locus_tag="ACG827_001887"
     CDS             10414..10881
                     /locus_tag="ACG827_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484020.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10878..11120
                     /locus_tag="ACG827_001888"
     CDS             10878..11120
                     /locus_tag="ACG827_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0484021.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            11138..11440
                     /locus_tag="ACG827_001889"
     CDS             11138..11440
                     /locus_tag="ACG827_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484022.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            11512..13566
                     /locus_tag="ACG827_001890"
     CDS             11512..13566
                     /locus_tag="ACG827_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484023.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13566..16178
                     /locus_tag="ACG827_001891"
     CDS             13566..16178
                     /locus_tag="ACG827_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0484024.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            16175..18706
                     /locus_tag="ACG827_001892"
     CDS             16175..18706
                     /locus_tag="ACG827_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0484025.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18758..19090
                     /locus_tag="ACG827_001893"
     CDS             18758..19090
                     /locus_tag="ACG827_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484026.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            19091..>19614
                     /locus_tag="ACG827_001894"
     CDS             19091..>19614
                     /locus_tag="ACG827_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484027.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILG"
BASE COUNT         7048 a         3061 c         3901 g         5604 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaatagta
      541 gatggcgcga ccagttttag aggcgatgga tatggatact ttgacaaaag gaaggtatgt
      601 acagctgtta gctcgtggtt attcacctaa agaagtattc aaagccttat ctaaaggaac
      661 agatatggaa aaagaaagat tgcgaaaaga attcgattat tggagaaccc ataatggaat
      721 caaggatttg aaaccagcac ggccagtaat taaacgaaag aaaaagataa cccaagactg
      781 aagtgtagag cgtatgtgat cataggcttt tttatttttg caaaaaacaa ggaaggtagg
      841 aaaactcatg aattatgaag aaatcgaaaa cagaaaaaaa gtgtcgaaag aaatggaaga
      901 aaagttacta aaaacgatga agcaaaaaca ccttaaaaga ttatctgtcg cgcaatatat
      961 caatgatatg cagttaacag gaaaagagaa agcttgtttg ttaggttcaa tgaaaaactt
     1021 tgaacagttg agacgaactt atagaattca tttttaaaac tttttctatg gaaatgagaa
     1081 aaagtttttt tgttttgtct gaaaggaggc agatggatga aaaaatggtt atttggtttt
     1141 ttaggagtca ctttaatcgt cttttgcagt gtgtttggct atgttagcta ccaaaaacat
     1201 gaaggagagg tgtttaagca aaatatagaa aaaaagatgc ccgtagatca aatcaatgca
     1261 catgcaaaga gttataagga ggatgcaacg aacgtcaata atgatatgtc tttggggcaa
     1321 atgttgtcca tacaaaaaga agcaatcgaa atgggcgtca ataaacaagt atttgcacaa
     1381 attcagattc ctgcacttgg tttagcattg ccaattttta aaggggcaaa tcaatacact
     1441 ttgagtcttg gagcggctac ttatttttat gaagatgccg aaatgggcaa aggaaactat
     1501 gtcctagcag gtcataacat ggaaatgcca ggtgttttat tttctgatat tcaaaagttg
     1561 tcattagggg aggtgatgga cttagttagt aacgatggcg tctatcgtta caaggtaacg
     1621 cgtaaattta tcgtacctgg gtactttaaa ttaatcgatg gcgttcctga agagaactct
     1681 ttcttgtctt tgccaaagaa aggagagaaa cccttattaa cgttgtttac ttgtgtttat
     1741 acgtcacaag gaaaagaaag atacgtggta cagggagagc tacaataaca aggttttaat
     1801 caacgcgagt cgttgaaata tgaaatgagg agagtaaaaa atggcaataa atcggtatgt
     1861 aaaaaaagct ggaatgcttg ttggaatgtt aggaatcatt gcaggctgct ttcaggggta
     1921 tcaagtacat gcagaagatg tcacgcaaaa aacaccacca gagaaggtca atatcacggt
     1981 tcataaatta atgtacgatc aaggaacgca attgaatgtt gatattgacg ggatcaaaaa
     2041 cgatggttat acgcacgatc aatatcctac aggtgtaacg aaatacaaca aggcagatta
     2101 tggtgatgtt gagtttacgt taggaaatat tacggatcaa gtattaccga gagaagattc
     2161 tgatctaacc aacgcgaaag tggacgagat tgtacaagat gtagaagaca agggatctaa
     2221 cagtgagtac gtcaagaatg caaccaacaa aattacttca gctgtagatg aaaatggtga
     2281 gatcactttt actgatcaac cagcctatgt caattccaaa gggaatgtat atgtggttta
     2341 tgaaagtaaa tccgctgctg gattagtcac acaaaaagca aaaccaatgg ttgtgattgc
     2401 gccaatgaca gataaaacag gatcaggatt tttaaaaaat attcatttgt atcctaagaa
     2461 cattgtttct aagttgagtt ttgaattaac gaaatttggt gacgatggcg cagaccaaag
     2521 caaacaaacc cttttaaaag gggcaaaatt tgagttatac aagggcgaac ctggaaaagg
     2581 aacaaaattg ggtgatcttg tatcggatga tcaaggaaag ctgactgcga ctgatttgac
     2641 gttaggaaaa tactattttg tggaagttcc ttcggaagtt gtagtgggat cagatcaaga
     2701 accaacagca gatcaatatt tattaggcgc agacgctcgc aacgatgcgc acaataaatt
     2761 aacctttgaa attacgaatg atggcgtgac aagtgactta aaagcttcat acgtaaacta
     2821 taaagcacca gtgatcgata aaacagtaac aaatggaaca ggacaagaac attctttcca
     2881 aattggtgac gcagtaaatt atcaaggaac gattcatatt ccaacagaca ttgcaggtgg
     2941 cgcagacggt atcacagtaa acggtgtgaa aagcgaaact tctccttaca gtgtatttaa
     3001 atggggagat acagcaggta aaggactttc ttatgttgct gaaaaagcag atattaaagt
     3061 aacaaataat gatggttcag tcgtattaaa agaaaatact gactataaaa tcgaaaattc
     3121 tgatagtggt tttgtgatcg attttatcgt aaataatgga caagtaagtg atacagttgc
     3181 gaatttacat ggtcaagatc taaaaatgac ttataacatg tttgtgaatg atagtgctga
     3241 aatcgcaaat ccattaacaa atagcgtgga tttcgtttat aacaataatc catttaatca
     3301 ggaagaacac cacgaaaaaa cgaaagctga cgttgtgact tatggcgcaa aattcttaaa
     3361 agtcgatagc ggcctatttg gaacaggtat caaagcaaca ccgcttgaag gcgcagaatt
     3421 tgcagctaag aatgcagaag gtaagtacta cggtggactt actgatacag ataaagatgg
     3481 tgtcaaagaa gcagtttggg tagacgatgt agcaaatgca gcaatcctaa aatcagataa
     3541 agagggtcat tttgaaatca ctggtttaac tgaaggagat tactcactgg aagaaacaaa
     3601 agctcctgaa aactatcaaa aattaacaaa agagatctct ttcaaagtag acaaagattc
     3661 atacaaagaa gaaaaccgaa tcacgatcaa aaataatcaa aaagcttccg taccaatgac
     3721 tggtagcaac ggtttccaat cttacgtatt gatctcttgc ttgttgctag gagcaggggg
     3781 actttctgca gttgtttact ttaagaagaa agcctagaag gatcagctaa tgattattcg
     3841 taagccccca aaaagaagag tcatgcacaa actgatgata tttttttggt tgctgtcggc
     3901 agtagcagtc tatttaattc cagttgtgga aacaaatgta gcgaaatttc aacaacagca
     3961 aagaatttta gccattgaaa atcttgaaaa aacagattct tctttggatg ttgcgaaaat
     4021 agagttaggc gatcctgttg gtgttcttac aattccaagt atttctttaa aactaccaat
     4081 ttatgacgga acttccgaca aaattttaga gaatggtgta gggattacgg aaggaactgg
     4141 cgatattaca ggaggaaacg gtaaaaatcc tttaatcgcg gggcacagtg gactttataa
     4201 agataatctt ttcgatgatt tgccttcagt aaaaaaaggg caaaaattct ttattaaggt
     4261 aaatgaagaa caacatgcct atcaaattta tcgaattgaa gaagttaaag cagaagaatt
     4321 acaaaaaaat tatttagatt atcttatgcc tgaaaaagac caagatagga tcacgttaat
     4381 gacatgcaca ccaaaaggtg tcaactcaca ccgcttttta gtgtatggtg aacgagtttc
     4441 cttcagtaaa gaagatttaa aactacagaa aaaagcaaaa aataggaaat caatttcatt
     4501 ggattggcga ttagcactgc tattcgcttt tttttgcgtt cctattattt ttatttataa
     4561 aaaaataagg aggagatcgg aatgaataag agaaaatggc tgtttgtttg tgtagcagct
     4621 ctcttttcta ctgtagctta ttcgcaactg tctttcgctg acagcagtga tagtagtacc
     4681 actcaaacaa tagataattc aattactgca ccaagcgaaa cggacagttc ggagaatact
     4741 acagaagaaa cggcagattc aaatataagc tttactgaat caacgaatcc aacgtcagaa
     4801 agttcttcag agacttcaga aaagccgcaa gacgtagaag atacgcaagt gatcaagcaa
     4861 gccattcata ttcaaaaaat tatttcttcg aatcaaatca acaatcaagg cagtcaagta
     4921 gcccagcaag aaggtatcaa tggcgcgaag tttattgtct acgatatcac gaatatctta
     4981 gaagaaatgt cacaagatga taatgagaaa aatcaatctg tagaaataat tgagtcaaaa
     5041 ttgaaagatc gagcaaaaaa actttcatac gaccagttga aaaaagtggc tgaaggagaa
     5101 accaaaacga ttgatgatca ggaaggcgtg attgaatttt ccattgaagt acctgcaaac
     5161 aaaaaacaag catactatat cgttaatgaa tcaagccctg aaaacatttc aaacagtgaa
     5221 gatattgttt tattgacacc tgtttcaaat gaaaaaggtg aatttttgac ggatgtttgg
     5281 ttatacccta aatcagagaa atcagagccg aaagaagaaa ttaaaaaagt tgtgtcaact
     5341 ggtgtaaaga agaacttttt tgaaaattgt tgggaatttt ttgctcaatt attttttgat
     5401 aattaggatg aaatgatgaa aataataaga atattagttt ttaatctatc ctttttagcc
     5461 gcaacaatat tgtgttgcgg tttaattttt tctcaattat caatttatcc aagatatcaa
     5521 cgattgttca ttcattttct aaacattcaa tgtgcttcca tgccattaga ggtgattaaa
     5581 aagctcttga tcaatccaat ttttatggtt agtttggttc ttcttacagg attggtaacg
     5641 tgggtattag gaaaaaaaga atgaaaagct tacgatcgtt taaaaaaata ttagatggag
     5701 ggcattatgg aaaataaaat tcattgcact acgggaagag taattacttt agtgttgttt
     5761 ttgttccttc ttctttttgc gaggggaact gcagtccagg ccttagaaaa agttgatccg
     5821 agtcaaatca aagaaaataa taatctggtg ctttatcatg atagtgattc attgagaaat
     5881 atcgaagtaa ctgggatcaa tggatttaag atactagatg agtattcaac taaaaaacaa
     5941 aaagtacttg cttggccaaa agtcaaagat ttgaaaagta cgtcaaaaat cgttgcccat
     6001 tatcaaaaag taggtactta tctaggaaaa gaaattgatt gtgacgtgac tttttctaat
     6061 tttgtagcgc acgctggggt taaccaagac ggattagcta aaacaattaa tggcaaaaag
     6121 tattataatt atttgctctt tgataaaaat atttatcgag gatttttcat gcagtcaatc
     6181 aatgtcatga atgcaaaact agaatttaag tatgcaggaa caaaaacaaa agtagctttg
     6241 agtgaaccac agccagaaat tcaatcaaaa atgagatcga tgagagcagc tggtattgat
     6301 ttaaacgata catttctatc catttacagt ttaaatggac agtatcagca acgtgtgtct
     6361 tggtataatg acaataatga atctgtcggt gaacgtaata ttgatatgag agaatttgtt
     6421 gggtatgaaa aaatgggtag cttaccttat tttgtcacaa cagatacagc atgtgcagaa
     6481 tacaaagctc ctgcgttatc aacaaacaat ttaacttcaa aagtagtggg aggacgtgca
     6541 gaaaaggctt atagctcgaa tgatcatttc accgatgttg taggagctga tacttatcac
     6601 agaagtggtg taacgtatac gcttcaaggc gcttccccaa cattcatgat tggcgcaaat
     6661 acgaatagta tgatgtttag ctttgatact gcattgctat ggacaccaca accatcgaag
     6721 cctacaaaag aagtgtttaa caaagctaat actgaagagg cagcacacaa tattgacaaa
     6781 aaagtgattc cacaaggatc agatgtttac tatcatattc accaaaagtt tgatgcatta
     6841 acagtcaaca caatgaacaa atacaaatca tttaaaatca ctgatacctt tgacagcaaa
     6901 aattttgata tggtatcgga tgggaaaaac tatgatggcg cattgtggta tgttgaagcg
     6961 aatggtactt ggaaaaaagc tgatacagga ggaaagttaa ctgtatcagg taatacagtc
     7021 acttatactg cttctgacag ctttttagct aacgctgcaa attacggtag ggattatatt
     7081 ttgcagctcc atatgagagc aaaaaatgat gcaaaaggaa ttgcaccaaa tgtggctact
     7141 gtaacgttta acaatgatcc gaacaaagga acacaaaaaa caaatacagt agaaaactat
     7201 ttctctctta aaccaaataa aaaagtggaa caagatggga aagatgttga tggtcgaaat
     7261 gatggtaaaa aaggaacgcc tacagctcct ttaaatgcag gatctgaagt acaatatcta
     7321 gtcacacaaa aatggcatac aaaaggtgta gacattgcct cagatcatta caagcaattt
     7381 agtattcaag atccagtgga agatcgtttg acgtataaag aaggttctgc gcgagtgata
     7441 gatatgagta caggaaaaga tattacttca gaaggtacac taacctatga tagcaattca
     7501 agaacgctga agtgggaagc ttccgctgat ttcttaagta aaaatctttt agatggacga
     7561 gaaattcagc tgatatttac agctaaaact cctttgaatg aagaattgga tatcgacaac
     7621 caagcaaaag tttcagtaga aagtattgag aaagcaacta atgtagtaac gattggggta
     7681 aaacctaact tacctcagat cattgtgcca aaaactggat cgaatcattt agtgctaact
     7741 gttattggaa gtatattatt tcttacttta gcatcattgg gctatgtagt tttaaaactc
     7801 aaataaatat aaaaacaagg ctgagaatca gccttgtttt cttttatttg aagattggat
     7861 tcgcactaat aaatacatta tctacgccaa ttctaccagc agagtcaact acaaaattat
     7921 gacctaatcc gagaatactt tggttattat aaagtaaatt tgcattatgg gcagggctat
     7981 ttttccatcc taccatggca tcgatagcac catcgtctga taaaaggtca tcaatataca
     8041 gcccagtttg acttatgttt tctcccttag caccgtagca gcctaagctt tccaataaat
     8101 cttggaaagt accaccacca gggcgatagt gactgaagct gacttgtaat tctttagctc
     8161 gaatatctgc agcctgttga acaatatcat tgaattgcat tggggcaaca ccattttgtt
     8221 ggcgataatc atttaaccaa gcaagttctt tttgacgcat aaagttacgt aattcgtcta
     8281 atgaaatctt tttatatttg aatgtgattg ttttattttg atcgacgtta ttaatggatc
     8341 gttggccgtt tttatccacg gtaacggtag gatcgtcatt tctgcacaag aaataatttt
     8401 gatcagtcat gtttttccaa ggagtagcaa agctatcccc ttcgtatact gtttttgatt
     8461 cactagagag aacagcgttg gtatcagcat ttacatgatt aacagtcaca ttaaatttac
     8521 gagtgtagtt gaaaatataa ttttgacttt cagtcacatt gtttaaggtt actgaatttg
     8581 ttggttctac ccgatcgtct aaaacatatc cttgaataga aggagcagtt aaagtgacac
     8641 tgtcaccgtg agtgacattg actatctgtt ctttcaaaat attaactcgt ccttcgtttt
     8701 tgtagttgtt attgcacaac aaattgattt gatgcacttg attataattg aatgtgattg
     8761 ttgtattacc tgtgattgtt ttgctttgag tatcattacc aatcactttg tagctaaaat
     8821 tatctttagc gccttcgcta tatttaaatg cttttgcctt agcagtgtaa gtagaaccag
     8881 aagttacttt tgcttgatct gtagcaatca cttgacctgt ttgattgtca atatggttaa
     8941 tagtaaccgt atattctttt ggtgtttcag gtttgtgttg caacccttta aatgcagcat
     9001 caagactatt tttagcattg tttacttgtg tttgagaaac cttagaatcg tctagtactt
     9061 ttttagcatt ggataaagca gtctgaaaat tattccaaga ttcttctgta aaatctcctt
     9121 tagcagttga ttttactcgg ttgtataaag cttgtaattc agctttattc acagtaggaa
     9181 ctggtttttt ctttaatcct ttgtaagcag aatctaattt atttttggca ctatccactt
     9241 gcttttgagt cgcttttgag tcatttaaca ctttcttagc attgtttaaa gcggtttgga
     9301 atgcgttcca ggtattgtcg gtatagtcac cttttttcgt tcccttaacg ctcgtataaa
     9361 gagcttctag agccgattta ttcactttgg gtttagaggt gtcagcgcca taccaaccga
     9421 taccttcgta tttccagcca tagtctttgg ttaaccattg tacttctttc caacttgcag
     9481 tgtagtgatg tgagccagat tttacaccag gaaggaaaag gcggtagatc ggtttattat
     9541 ttttttcggc agaacgccag gcaggccctt cataagtcca gccgtaattt ttagtcaacc
     9601 atttcacttc atcccaacta gctgtgtaat ggtgatcacg aagaccttta ttgtacaaac
     9661 gatagacaag atggccaccg ctagtcggtg cttcccaggc ggggccttca taatttcccc
     9721 attttatctt tactagatgg tcacgttctt ttttgctaga ggtgtaaaaa tgttcattat
     9781 tatttggatt gtaaaggcga tacatgacgt tagttgaagc agcgtcaaca gatccagcaa
     9841 gactaagtga ggcagcaaat gttccaatag ctaaaacagt ttttggatta aataatttca
     9901 ttttttcaac tcctaaattt aattaaaaat gtttacatga ttattatata tcggctaacc
     9961 gatatagtca ataaaaaata agaaaagcct tatataattt tggcttttct tattttattt
    10021 gttagttgaa gtattaatat caacgatttc ttcagccaat ttatttaatt tcatagcatt
    10081 tatgaatctc atgttgtcta tactagagcg tttgttggca aggtcactta ctgtagttaa
    10141 gggtacgcca gattctatgg atatagaacg aactgattta ccactatcaa acagcaacca
    10201 ttcgattttc tttttatcag cgtacatttc aatgctcctt cttgtttttt ctttattata
    10261 gctctttttt gtaatgaatc acaatatcaa gagaaaaatt ttctatagaa aggtggattt
    10321 ttatgagtat tcgttctccg tgtatccaaa tgaggtttgt tgctactttc gttatttgat
    10381 atactaaaaa taaacaacga aagaaggaat aaaatgaaga aaaagcaact atggtttgtc
    10441 tctatgtttg caatattttt tattatttta agtggatgtt caagtaataa gaaatcggaa
    10501 gctaaagaag ataatagttt attgcaacca ggaactgttt ggaaagaaga agtaggtggt
    10561 cttattcata atttaaaaat tatagatgat actacctggg aatattctga aagtgtatgg
    10621 catccagatc ctgtacaaat aacagttaag aggcaaaaag actatgaagg tttagaaaga
    10681 tacaaaatag tagattctgc aggcattagg gaatttataa acaaacctga ttcattattt
    10741 attgttgttc cctatgagaa ggatggcgta aaaaaaataa tttttcttgg atctagcaag
    10801 gatgagaagc gaacgaaaga gaaattaatt catgatggat cagagttaaa taaatacaaa
    10861 ttacaaaaga ctagtgagtg attgctagtc tttttattta tcctgaagga ggaatcacag
    10921 tggcaaaagt taaaacacca ttcgaaaaag ccgcgtctaa aatcttagaa cgccgtggtg
    10981 tttctgaaat acagtggaaa aaagaagttc ttgaaaaagc acaatttgac ctattacgag
    11041 gaaaagataa ggacttagaa gaatatatct atcaacgtga acgtgaaaaa cttgtattcg
    11101 atgaaattat taaacattaa aaggagcgaa taatcttatg tttgaatcaa ttacaatgaa
    11161 ttttttaact caaccgaaag tagtagctgc agttgatatt tttagagcat tacaaactgc
    11221 cgcaactagt ttgaaaaata atctgatgat ttttgcggct gtagctgccg ttgcctccat
    11281 tgctcttgga ggtattttct tcttctttgg gcgaagagga gctgaaaacg ggaagacaca
    11341 cgtaggttat gtattgttgg gggtaggagt tattttagct gccgcttcta ttgtagtttc
    11401 aatgttctca atgtttggac aaaccccaca aggactataa aaaaaacaag taggaggagt
    11461 aaaaagtacg tggctacttt ttactccttt ttttatgaaa ggagaatatt catgtttttt
    11521 acgatttcag gaaatgcaaa attggataaa gagttaataa gaaaagcaac gaagaaagtg
    11581 ggaaagcgta cttccttttt accgaaaaca attaaatatt ctgtcattga aacggaagaa
    11641 cagctacatg tgttaggaca agtgttagaa caagagaatc aatcttctgt taaattgact
    11701 ctcacgttac atgataaaga aggaaaaata gaaaaggctg gcacagaata cgtttttggc
    11761 ccactggatt atcaaatttc agaaggtttt tatcctacat tgatagattt aatccgcctt
    11821 gatgtgttta aagatacgga tgaaactatg agtgtggaag agtcttatcg aaacgaaaaa
    11881 ctgctacaag ttttagaaga agagttagag agcgaccaaa gaaaagtgaa tcaagtgagt
    11941 gaagtcgaag taaatgaaga agtaccatgg gaacgaccaa tctttccaaa gaaggagtca
    12001 aaagaaaaag agacagtcta tgttgacgaa caagctgata ttccgactca gaaatcgata
    12061 tttgaggaag agaacgatgt gatgggtcaa ttggaggtag atccctttga agaaacaatg
    12121 gaggatgaag agacggacga agtagaagtt gatccagttg aagctaatga agagtcaaca
    12181 tggactgaag gggattttca ggaatatatt gaaccactta tcagtgaaga agtagaaaaa
    12241 aacgaaacag agatacaaaa ggcaactgta tttgattata ttttggatca atatccgcca
    12301 gagcatgaat ggatgaaaga aaaaaacaaa ttatttttaa atgagcttta ttcgaaatat
    12361 caattacccc aaacctatca agcattttta gagagtaaag aagaattaat ctcacagggt
    12421 aaaggcacac ttttagaaca gttagagtct gtccaatcaa caaattggca tgagcttgca
    12481 caagaagatt tattagccac ctttgaagaa cgtcaacaag aaacagatca agaaattaat
    12541 gagtatgcga cggaacagtc gtctaattgg gagaaagtaa aagaggaact aaataaagaa
    12601 gaacagcgag tgattgaaga agaagtacaa aaagtcaaag agcggtacga aaagaaacgc
    12661 gacaatgctt attccttgtg tgtagaaaag atcaacaatt ttactaagac gaatcaagaa
    12721 gcgttatcga aagaaaaaga aaacttgtta aaagaacgta ttaaagaacg aaaagaagag
    12781 ttctaccgcg gtttacgtac agataagcta aagattagca aagaattaaa tgaacaactg
    12841 aataagctat atcaaacaac gactgaagtt cttatggaaa aacacgaaca gatccagcaa
    12901 gaattacaag tacaaattcc aaaatggaaa aaagagtatg aaattcaaca acaagaattg
    12961 aaagaacaag aagaaaaaca acggcaaaaa gaaaaagagc gtgaacaatt agaaataaga
    13021 aaacgggaac aggatcttgc ggaacaaaaa atggcgatag aaaaacaacg gttagagcaa
    13081 gaaaaggaaa aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcgt
    13141 caagcacagt tgaattatct agcacaacct caaccagtag ctgctgttca aacgccagtg
    13201 aacaaccaag caaatttaac tccttcaccc cagcgttcct ttaacggatg gatggtggga
    13261 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
    13321 aatcaatctt cacaggctga ttctgtagcc acaacggcgt caatcaagga agaagcccag
    13381 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
    13441 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg atcctagtaa ggctaaagaa
    13501 ggatcaagcg attcaactgt acactcaaac acttccgaaa gttcagaatc aaatgaggtg
    13561 aaataatgcg tattagagaa acaacgagac gaaaagacaa acgattcaat ttagaaaaga
    13621 ataggtatct ttctcatttc tatcgctata ctccttcagg agaagagcta ccagaaaaac
    13681 atgacaaacg tatttctcaa aagatttttt atagtggctt agcttatctt cctttaaact
    13741 atgttgttac aagaatacca gaagcatttc atcgatttga gattattcca ggaagtttac
    13801 aaggagtgaa tatttttcag ttacaagaaa tgccgcagtt attccatttt gattcaactg
    13861 gtttttggaa tcctttgacc ccaacaacaa gtttaggcac acgtttagtt gtttctacga
    13921 tttgtgttgt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtccgctag
    13981 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
    14041 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
    14101 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
    14161 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
    14221 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
    14281 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
    14341 tgagtgataa tccattggca ctcatcgtga aatactggaa acgaggagac atagatactg
    14401 caacacagtt aaccaatact ttcacaaata cgatttatta tgatgccaac gcttcagata
    14461 acaaatactt taacgaaaac gcgcagaaag ctgtcaatgc gcttattttt gcgttgttag
    14521 aacaagctga tcaaacagga gattactcca aattgacacc gaataatctc gttgagcttc
    14581 tttctgagat tggcggtttt aattattatc cagatccaga caatgaattc aaacagttga
    14641 atgccttaga tgaatttatg aatcagttgc cacctggaaa cattgcaaaa aaacaatatg
    14701 gggcaacgaa aattggatct gataaagcga agggaaatat tttatcaacg gcaatcacgg
    14761 gattgaatcc atttactcta acgaaaattg caaagatgac ttctcaatca acgattgatt
    14821 ataaatcggt tgggtttcca aaatacttag atttgaaatt acatgaaagt cttttaaatc
    14881 aacgagtcgt agtagaattt tatcataagg gaaagaaatt tcatgaagaa cgtgtcaaag
    14941 taggctataa aggatttagt gaaatcaatt ttgattgtca tttagttgca ggtgatcaag
    15001 tttgtttaaa atacgatcat caaggaaaag aatatgtggc ttggtatgaa ttttctcaaa
    15061 gagtattgaa agacgaagag ggtaatatcg tttatcggaa aaaacgagga gaaacccatt
    15121 tgccagaatt agaaaaagaa gccgttctaa cattagatga ggaaaaaagt aacttattag
    15181 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg cttacacctg
    15241 attatgatcc atctaaccat atcattgtta gtatttttct tagtcagtta tataaagaat
    15301 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgcaggatt cattttattt
    15361 tagatgaatt tgggaatatg ccaccaatgg ataatatgga aggaatcatg acggtcacag
    15421 ctggccgaaa catgttgtgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
    15481 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
    15541 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
    15601 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
    15661 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggagaa acgattgtac
    15721 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
    15781 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
    15841 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
    15901 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
    15961 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatcttttg
    16021 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca gcagatcgac
    16081 ttcaagaata tgttgaaaag ttgatcaaaa ataaagaaat ttctgaaatg attgggaaac
    16141 ggttgttatt ggaatctgaa aaggtggtga aagaatgatc attggaggaa ttttttctgg
    16201 catagccgac tggtggtcag gcttattcgg agaaggggat gcaaacccca aaaccgtaac
    16261 gaaagtctta caagattatg ggaattatct gcagtatcaa gatgtattta gttggatttg
    16321 gaacactttt ctgtggggat tgattaaagg attatataat ctcaatactt tactagaaaa
    16381 aaccatttat caaagctttg atttaaaaga cttattgaac gccgctgggg taaatgcact
    16441 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
    16501 aggcttgaaa ttttttatga gtaaacaccc accaaaagtc aaaaatatct tggttaattt
    16561 atttatggcc atgatcttga tttttggtgg tagttcttta atcgatgaag ggttaaatat
    16621 cagtaaaagc ttttacggag acgtcactgc agcaaataaa gcagataaaa cgggatcacc
    16681 tgcttttcaa attattcaaa ataatgtgat cgatgtcagt acgttactag caactgaccc
    16741 gaataaagta gaatccctac caacagataa aagaaatgct ttaacagcga aaaattttaa
    16801 aacagcgaat atcaatggga ttattactcc tgaacaagct aaaggtatgg caggtaatga
    16861 taaaagtggg ttatcagata tacaaaagga tcggttaaag gcattacaat accgattgga
    16921 actagatgat aatgggaaag aaattcctgt tcaaattagt gacgaaggct tagcaaaata
    16981 tgtctatact tcagggtatc gtcgatacag tagtaaacca ggtattattt tagctggtga
    17041 atcttctttg gcagtagcct atttatttat cctatttacg atcgttactt gcattattga
    17101 gctggtattt aaaaaattct atttagtgat tgccgcgtca acggactttg agacaggtca
    17161 acggatgaaa agtgcgatag aagatatttc tcaatccttc ttgttattgg cgtttacgat
    17221 tttagaatta cgaatctata ccttaatgct ttcaggtatc ggtgatttgc atgcggcagg
    17281 aaagatcaat ggattcttgt atgtcgttgc tttgattgta ctaaccattg cgctattcaa
    17341 aggctctcaa tcagttacga aaattttcgg cgttgataca agcctgaaaa acggcagtaa
    17401 ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa
    17461 aaaccttgca agtgctggta agagtggttt aagccattta aatgatattc gtaaaaatgg
    17521 atttggccat cgaaaagatg ttacccctga taacggggaa gaacaatcag acgataaagc
    17581 tacaactgta cctaatagta agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg
    17641 tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc
    17701 tgaagatctt acagaaaaag ccaaagacac cttagaagat aaattagacg gcacgagagc
    17761 taaagaattg gctgaagctg tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa
    17821 agcttcagaa gtcaaagaag gactaaaaga tgcgtatggt gaaggacaag tcaatgcagc
    17881 agtgaaagct aatacaacaa ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt
    17941 caaagatcac caagaagcgt tgcactctaa tgaaaattcg attgaagaga aaaattccat
    18001 agaagaaaga aattcagaag aaggaaaaaa tggacagtta ccaaaaactg atccagaaca
    18061 atatcgctat aaacctaaca gtgaccttga aaatgagaag cgaatttcag atttatcaaa
    18121 agtaaatcaa gaagacaaag cagaaaatga gggacagtta ccaaaaactg atccagaaca
    18181 atatcggtat agatctgtta actccgaaaa tgaaaaacgc atttcagatt taccaaaacg
    18241 aaatcaagaa gtacaaacgg atgaggattc catcgttctg ccaaatgata atggaatcga
    18301 agttggaact gaaaaatcaa cgaaaattgg tttacctaag gcacaaaatt taccaatgaa
    18361 agatagtcgt ttgaatcaat tcaaggataa tgatcaaagt tttgttcatc aatcacctgc
    18421 aggtactgtt caaatgaaac caagtcgttt gcctgaaaat ggaaatctga caactgtttc
    18481 ttacaattta tctgaaagtg gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag
    18541 agaagctgca ccaaaaccta ttcaaaacgt tcatacaggt tacaagccag caaatgtggc
    18601 atttactgca ccaacaaaaa taaatgtgac aagagaaaca aaaaacagga acaatgcatt
    18661 gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa ccataaaagg cctacaagtt
    18721 aggccttttt tctatgtatg aaaaggagaa atcatttatg gataaatata gtaacatgaa
    18781 aaaccctcca agatttagtg gagaatggaa gtttttcgct tttttctttt tagatctatt
    18841 aacgcttggt ggctgctttt tttgggcgaa aatggtcaat aatcttgtcc acttaccact
    18901 cttttttgca ctcttgaatt atggcttatg gattgcgata gcgttagtga ttgtttggcg
    18961 accctataat aacccaggtc aacgacaaat ttttgcgatt atcgagtcta ttttttttgc
    19021 ggacaagaac cattatcatg cgttagatcc taaccgaaat tatcatggta cgtataagga
    19081 ggatgaataa atgcgagtca aaggacaaaa ttcagttgaa aggaaatcca ttttgcagtt
    19141 tggaaaacca gaagagcgct ctatcttgga cttatttcca tataaatcct gtacagaaga
    19201 gggagtctta attactagag aagatcattt tcaacgttat tatcgtgtag tttctacaga
    19261 cgtggaagga ttaaatgaac aagagaaact agaacgaatg aatcagttga ccaccgtcat
    19321 gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg atctaacgga
    19381 acaaatcgtg caaaagcggc aattattaga taaaaatcgt atggaacagt ccttaggaag
    19441 aaatctacaa cgattaaaaa aatatgaaaa gaaattagtc gaagaaatcc aggaattgaa
    19501 acgagcagaa caagaacgtc ctgacttaag cttctttttc gtcattgaag gaaagacaat
    19561 caaggattta caaaccagaa accgtcagtt aatgcgatct agtggcattt tagg
//
LOCUS       DAZKOU010000057        18735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000057 DAZKOU010000000
VERSION     DAZKOU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.058"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..1032)
                     /locus_tag="ACG827_001895"
     CDS             complement(121..1032)
                     /locus_tag="ACG827_001895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0484028.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1409..2047)
                     /locus_tag="ACG827_001896"
     CDS             complement(1409..2047)
                     /locus_tag="ACG827_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484029.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2051..4033)
                     /locus_tag="ACG827_001897"
                     /pseudo
     CDS             complement(2051..4033)
                     /locus_tag="ACG827_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4213..4878
                     /locus_tag="ACG827_001898"
     CDS             4213..4878
                     /locus_tag="ACG827_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484030.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5275..7308)
                     /gene="brxL"
                     /locus_tag="ACG827_001899"
     CDS             complement(5275..7308)
                     /gene="brxL"
                     /locus_tag="ACG827_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0484031.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7426..9948)
                     /gene="pglZ"
                     /locus_tag="ACG827_001900"
     CDS             complement(7426..9948)
                     /gene="pglZ"
                     /locus_tag="ACG827_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0484032.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(10064..13696)
                     /gene="pglX"
                     /locus_tag="ACG827_001901"
     CDS             complement(10064..13696)
                     /gene="pglX"
                     /locus_tag="ACG827_001901"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0484033.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13760..17431)
                     /gene="brxC"
                     /locus_tag="ACG827_001902"
     CDS             complement(13760..17431)
                     /gene="brxC"
                     /locus_tag="ACG827_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0484034.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17456..18031)
                     /locus_tag="ACG827_001903"
     CDS             complement(17456..18031)
                     /locus_tag="ACG827_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0484035.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(18031..18630)
                     /locus_tag="ACG827_001904"
     CDS             complement(18031..18630)
                     /locus_tag="ACG827_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0484036.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5786 a         3568 c         2620 g         6761 t
ORIGIN      
        1 tttaaaataa aaagaactaa atcaagtaat aattaaacta ctgttgattt agttctaata
       61 aaaagattta gatgaacagt tttattgatt taaatagttc atatttttta tttataccgc
      121 ttatttacta cttgctgtac tagaatctga agtgaatgta ctcaaaacat tttcaaagtc
      181 tttgtctttg atttttacat tagcgtcttt caatacttcg ccaattactt tattctggaa
      241 ggtactgtca cttaattttg tgtctgttgc aatttctttc aattcttttt tgtatttatc
      301 catgtcgttg cctttgtttt ggtttttcac cattttaacc acataatatt ccgtagtata
      361 agttgaagca ttggtagaag tgatcacatc agaaacttga ccatctttca acttaaaggc
      421 tgcttctttg acttctgccg gaaccgttgt agtagtggaa tcaaatttca cttttccgcc
      481 atcttctttt gtcgtagtat cagttgactt gtctttggct aatttagaga aatcgtctcc
      541 cttatcagca gctttcttca catcttttgc atcatcttca cttgctactt ggataatttg
      601 agcttccact tctggatgga aagattccca agccgttttt aaatcttcat cggtcaattt
      661 gatatgtttc tttagacctt cttgaaaggc tagactttgt ttgatactgt ctttgaatgt
      721 ttgttctgtg taacctgcag atttcaattg tgaatcaaat gaatcaccca attgtttctt
      781 tgtttgatca tattgggaat cgatttcttt ggttgatacg tcatctccat atttatcttc
      841 aaatacttga taaacgatca actgttgcac agcggaatct gctccttgca tttttttcaa
      901 gctatccatt acttcttgtt cgctgattgt accgccttta taactaacgg ctgttttttc
      961 accggcattc gtacagcctg acaataagcc agtgcctagt acgatggcaa gtgctaaaaa
     1021 tctttttttc atttatcaat tctcctactt ttcgtttttt gcgaattgct aaaaacaaag
     1081 gtgttcaaga tgaaaatcat caagaacacc tttactaatc tgctcgatac gtcctgaata
     1141 gatataaggc tacacaatcc agaaagttcg ctcagcagaa taaatgtcaa gtagcacatc
     1201 acaccttaat atactacttc ttttgttacc gtttttcaag tctatcttgc gttccgtatt
     1261 taacgtaact aattcttaga acaaatgtta tcgattttgt ttttcttctt ccgctgttgg
     1321 gatattcaaa tcagacactt ttatgttcga ttttccagac atttttttgt tgtttttatt
     1381 agaaaaagtt tgttttttca tttgttttct aaagcatgat cttcttgttt cctattttgg
     1441 tcagatatgg atcatgcgtg accacgataa ctaacgtacc ttgggaagcc tgttgcgata
     1501 agatttcaac gacttgctcc ttatttcgtt tatctaatga tccagttggt tcatcggcta
     1561 aaatgatttt gctattcttc aataagactc tcgcacaggc gattctttgc ttctcaccac
     1621 cagataatgt gtagataggc gtacttagct tcttgtttat atttactttt ttcaatgcct
     1681 catactttgc tgttcgctta tctgaaacat ttaaattcga aaacttcaat ccaatatcta
     1741 aattaaaatc gattgtttca tattcaatca acccaaaatc ttgaaaaaga aaagagaggg
     1801 catttctttt tatcaagcgc ttctctcttc cagttaaatg ttttgtttct tgattaaata
     1861 aataaaattg ccctgaactg aattgatcat tcaatcctaa tatattcaat aaagtacttt
     1921 tccctgctcc gctctctcca actacagtca caaaatcccc agcatccgca gtgaaacgat
     1981 ccaaatgaag aatcgttttg tttcgaatga tttttttcgc accttttatc actatcaacg
     2041 gctccaattg ttaacctcca ttcatcagca ataactgact tttcttttct gtagacaaaa
     2101 taaaaacaac tgaaagaata aaatcaatga agctaaagaa aaaaatatta aaaagatatt
     2161 taggctctct ttctataaga aaaaggaaga ttacacttgt cgttccccat ttgatgaggt
     2221 tcatcatgaa gaaatcttta tacgtttcaa agagcgaata accacttgaa cgaaagatag
     2281 cgattttccg acaattgtag acaaaatata tgcataacat gacatagctc aacaagaaga
     2341 aagaaaagaa agtgagtaaa aatatctgta tcaagtaatg aaatgatctg cttaacactt
     2401 tgacagctat ctcttcagtc tgagtatacg gaaatgataa ctgcaagtta tcacgtaact
     2461 gactttcttc aatcagctca aataaccttt ctttcgacaa agagcctatc ttgatcttta
     2521 acggtggata gatttctccg ctgagaatat ttctttccca atacgtactg tttttcaatg
     2581 tcagtatttc aacaaccgga taatgttcga tccaggggat agaaggaata aatgtataaa
     2641 tgggttgatt gttttttata aatagaaatt tcggttcttt ttcactgatt tcttgataat
     2701 attctgttat ttgcgccagg tcatttttta aatctgtatg tctatatcgt tcagggatca
     2761 atataacagg gtgcgtttct ttttcggtta tttggatcaa ccttccagag acatcaatca
     2821 aaggaaattt cttaagataa ttcagattta tttttatccc tctgccaaac tgttcatttt
     2881 cttcattcaa gtaatctgat acattgacta atatacttcc gtttttattc aaagtttcat
     2941 ataattcctg atcggctgct gatgcaaaac gattggaata aataaaatcc acgtggtttt
     3001 tcccgatggt tactggataa aatacatgat agtcctcacc agcgatttct tttggtgttt
     3061 catgggaaac agctaactgt gaagaagaaa aaacgatagt tgctaattcg acattggcaa
     3121 cggtcaaaag aagagaaaac aacaatccaa atgtaaaaag aatgtattgg atatagttga
     3181 acgcttctct taacgaagcg attttttcaa ctaataaaag agaaatcaaa gagataccct
     3241 atattccgct gaacaagaaa acgtacatct ggaacaaata aagaaattgc tccttataat
     3301 acataaaaat aaaaccacca atgattcctg caattatgaa tgtaataatg attttctttt
     3361 tccccaataa acaccacaat acttgatgta atgaaaagcc attattcaac aataaactga
     3421 tttctttatt catcaataag aaaaaggatg ataacaaaag gaataaaaga atcaatccaa
     3481 tagttaaata cactcgataa ttatatacgt ctgtacttga aataaccgaa tacgaatagc
     3541 tattttcaaa gtcttcaaaa atattagtgg cataatcagt gtgaaatgct tgattgaatc
     3601 gttccacaaa tagtaaaatg aaccgttcat aggcttcttt ttctctcgtc atcaagaaaa
     3661 attcaccttc aaaatctttt attttgatcg attcttcaat ggacgttacg cgaaaggagg
     3721 tattagaata gggtaccttc tcgtgagaag atttgacatc cccttgataa tacaatgtca
     3781 tttcaagctt tggctgtatc ttgaagttcc aagatgtatc tacttcattg gattcattta
     3841 ctgttttttt catataagaa agctcagttt ctgatgagac attttcaaat accatgatta
     3901 gctgctcata ttcttcttgc ccaataaaat gttgtggcaa tcgaatcgga gtataagtat
     3961 ctgtttgatg gaaaaattca tttgtccgat agagtacctg tttatttata ataaccaagg
     4021 caaaaccact catgataccg gtcactatga ttgtgagtaa aactgtgaac ttttttttga
     4081 ttatcaccac tctcccttac ttaaatgatt ctttgataaa atcataccgt ttttcatcaa
     4141 aaaaacgtat ctatcaaatg tataggtcat gtagaataaa tctatatact gtagaatcga
     4201 aaggagaaga agatgaacat cggagaaaaa atacgaactt accgcagaaa ttgtaagctt
     4261 acgcaaaaag aattaggcga aaaaatcggt gtttccgata aaacaatctc tagttgggaa
     4321 aattctcgta caatgccaga ccttgaaatg ctgtcacttt tacatcagac tttaggtctg
     4381 ccgattgatt ttccttctat tgaatcagct accgaatcaa tagaaccaac attgccggat
     4441 gaacctcttg ttatcaagaa atcttattca tcaaaacaac ccttctcact caaaaagaca
     4501 ctatctttct ttctgctcgt cttttgtttg gtctatcttt ttcttatcaa ttatcaatat
     4561 aaaaatgaaa cggttgatcg attcaatcct tttttgaaac cacaaatcgg ctataccaca
     4621 ctccctgtag gatacgtgga aaattccatc gaacattggg tatgtaatga tatctttgtt
     4681 catggtacat ggctgtcttt tcacgctgga gaggtagaaa aaggtaagaa tcatgctgta
     4741 atcaaacaca aaggaaaaca cgtcatgaac gttcgcctgc tcacttggga agccattccc
     4801 tcagattacc aagaaatcct catccaagac tatgacgatg ccttaaaaag aagccctcag
     4861 gagccagata ggcattaagt aggagatgaa cgtagagata gaaaaaatca atgcacatga
     4921 tcttatcaaa aaaaaacagg ggtacaaatt aattgaaaaa gctttgagat gaaagttcct
     4981 aaataaaaaa gttcagaatg ataggtaaaa aaatgactaa ctattctagt attaggctat
     5041 ttgtattatt cttctgttat ttttatatca aaaaatccag aaataactaa atataggtat
     5101 ttctggctta tagtataata tttacgccac agcccccaat gattcttttt ggaatcatga
     5161 tcaaggacgg acatacacaa aaatattttg cccattcgat ccgaatagat taacgactaa
     5221 tctagtaagt aatatagatt ggtacgtagt caaaccagca aatggaaaag ttgtctattc
     5281 caccccaagg gctttaaaga cagcatcttc cggcgtttga taaaaaatca aattaaaggt
     5341 acccattaat tctgggggta cagttccaat atcagcagcg cttatcatag gtacaagtac
     5401 ttttttcgct ccggcatctt ttgctacttg taaacagtta gctaaattat ctaccttaat
     5461 catagaacca ccaattgtaa tatcccctaa aataactaaa cttggtaata caggtttatt
     5521 taaggaaact gagcataaag ctattatagt tgataacgat aatgtttttg tcataccaat
     5581 gccattcata tcttgaacat gaacgacaaa atcttttgtc gtcaaactaa tagactgact
     5641 aacacttcca taattagctc ttaaatatcg gtatgccgaa tccatcgctt ctcttgctcc
     5701 agcatctgaa ttgatcccag tacgttcaaa ctttccattt cccgaagtca tttgcgtttc
     5761 caatttatag gtacctaatt gcccagtttt gccataagat accgtgtaca aatgccctgg
     5821 cttccctaac ccttctggaa tcaaagttcc tccaccttgt tcaggaacag aaacatactc
     5881 ttctcgctct tcttctaaat caatgtatga aaaattgaca tcataaaatt ccattccacc
     5941 aattttcttt aattgttctt ttacacgacg acgcatttca agagcaaatc ttagtatttc
     6001 ttcgacatca cttttttcaa atctaccatc aggatacaac agtttcatca tcccagatac
     6061 aatccgacga actgcaatcg tatcccgctg atttaattga gaaccaagtt tgaaatattt
     6121 atcaaaacta tcgccaaaac taacttttcg aagctcacgc atgacttcag aaaaataatc
     6181 tgtaatgaag ccatagtcat ttgtaaaaaa atctggtcga tatttaggaa tttcccaacc
     6241 tggtaaataa caatgcatcc gatccaaaaa ggctgaatcc gtccccataa cctccggaaa
     6301 tggctcaaat aaattcgaag ttttcaatat tacatcaacg ctttgattaa tatttcctac
     6361 aaaagccatt gatgctgaag cattcttttc ttctttgcct ctagcaaaag atcctgaagc
     6421 catgtaatct ttcatgattt gaacgccatc attatcctta aatttaatcc cagcaacttc
     6481 gtcaaaagca acacagtccc acatacctac aagtcccatt gtacgactag ccatattata
     6541 gaaaaggttt gctactgttg tttgtccacc tgaaacaagg attgagtttg gtgaaatttc
     6601 tttatataca tgcgatttac ctgtaccacg tggtcctagt tcacagagat taaaattatt
     6661 ttcaattaat ggaataagtc ttgctaaatg aagccatttt acccttttat taaattgtgt
     6721 aggttccata ccaatggacc gtagaagtaa gtcaatccat tcatttgtag taaactcctt
     6781 gcgagccatc ttgtaagcct caatgtctaa accaggcatt tggattggat caaccttttt
     6841 tgcaataaaa gggctattcc aacgatcatt ctcatcatac caatattcca tcgaaacaat
     6901 acaccagatc cccccaccta ataatcgatc aaattttttc ggatactctt cactaatttg
     6961 aacatttttt aatcccaggt tagagaattc tgcttcataa caatctttct tttcatttaa
     7021 atgtacagtt actttatcaa taactgtata accacccaat tcacgaattt ttgcttttac
     7081 tttttctgct tcatcaggac gcacatagtt tcgagcaaga atatttttaa cattttccac
     7141 acctttttca accaatgttt catccaatga cgaagcatac ataccaagta aatattccaa
     7201 tacataaaca ggtacattcg caccttcttt aattttcttt gttaaatctt ttctgacaat
     7261 ctttccggga aagaattcag ataactttaa aatttcctgt tgttccacta gaattcctcc
     7321 atgtgttcag tcattataat agcaattaaa gtatatcata cttaaccagt aggcttgata
     7381 atacaaaaga aaaagtatcc acaaaaaatt gtggatacct cgtttttata tatcaaaatc
     7441 aaatgattct gcaaaaggat tatctataat gaattttatt cgctcggtca aatcattttt
     7501 ttgatttttt atcactaaat aataagtatc ttcaactgta tatcgacgat ttacaaattc
     7561 aaaaacaaat ttagtaaacc gctgatttgc atcttctgtt tttgaatccg caacatagat
     7621 ttcttcattt gagatacgtt caccatattc atcttcaaaa taaagccaga aagatgcggg
     7681 gtcataaaca tcactaattg catttttttg atagaattct aacgtggttg ataatcctgt
     7741 aattctgcgg ttgctagtca ttaattcaat tgctactggt tcctttgcat tgctaccttt
     7801 ggctaccgta atttctagaa cagggactag catttcttga atagatgacc ctccatgaac
     7861 gtagctttgt cccccgccag ctttcttaaa aatacttgaa gtaatcggga cacaaatgtt
     7921 tctagtgtca tcatttccta aagaatctcc caaagagtaa tttttcgtac caattatttc
     7981 gtattcttct tcactaagaa taaaacgacg ttctacacga tctttttcat ttctagatgg
     8041 attttcaatc ttatctactt cttcaacgtg gcttctttta taaataaatc catgatcagc
     8101 cgtaactata aaacgataga tactgccatc attatgcaag cgattaataa gtcgggaaat
     8161 ttcgcttatc gctaaatcac aggcctcaaa aactcgattt tcattcgagt gatcacctgt
     8221 tctatccact tgatcgtgat aaatataaat gactgatttg ccattaaata cttctcgtac
     8281 ctcagcctta ttcatcttaa ccacatcatc ataatttaaa gctacagcat ttgcatctcg
     8341 ttgctggaga atcgcatttc gattggataa accattcgtt tttttcccat caatcagtac
     8401 ttctgttcct tcattgtaag ttagttgatt gtttggtaat aaggcggctt taccgaattc
     8461 agtaactgaa ggtaatacag aaagaaagct agccatgttt gttgaatact tcttttctct
     8521 aactaactta cgttctaaat tcatccctaa ctcatatcga aaagcatctg aaataataac
     8581 tacagtttta acagagcgtt tcattacagc ttgattataa aattttcgaa tggatggacg
     8641 attcttaaat tcaaaagttt gattccaaat ttgtcctgat tgatcaagaa acttcgaata
     8701 atttgcttca actaatcgat acaattggcc atatttttgc ttttcttcta tatgatcact
     8761 attccaaata aatttccgat aatgcgtatc aacccaatac atttccttct catattgagt
     8821 tagcaaatta tccatgttca tcggcaagca caatctgttt ttatccacaa gataataagc
     8881 atgtttcaat aattgatact cataaatata atccgatgcg aaatgcattt tttcgcgctg
     8941 ctttaaacta tcaaaaattg tttgtcctgc aattttcgtt gtagtgtcag aagcctttaa
     9001 tcgttctgca taaaacatta atatttttgc atgaatctca gaaaatacgt ctgcttcaat
     9061 tagatcatca atttctatac tgtctaataa tccatcacca tcaatagcct gatatacttt
     9121 tttagatagc tgttcaaagc tttccatgta acgtgtatca ttcataaact gatccatgaa
     9181 agtaataatg tttgctttca aatgtagtat ttcaaatcct ttgaacttct tcggcatctc
     9241 ttgatcaatt tgataataaa aatagttcaa ataaagacac atcgtcagct tttccaatgt
     9301 tggctggcta tccgtaaagc caaatttcat ttgaataaat tcccaaaatt tagctgttaa
     9361 ctgatactta tcaagttccc ttaaaggatt ctccctactt tctgtaaagc tttttagaac
     9421 tacacgcagt aaatcattta atcgaatact tttcgatttc aacaaaacag ccattatcat
     9481 taattcaatc gtttctgatt tattaattgt catatgcgta ctaaattcac caaaacttct
     9541 catgcgatcc tttgcattaa agaaattacg atactgttta actgtctgat gaaatctaag
     9601 tggaatagcc aattcattca tgataatcgc taagcggtct gcactaaatt cctctgaata
     9661 attcaataca ctcaacaaat gattatcttc atctttatca tcccaacgag gaaatggcgc
     9721 atagattaaa tagttagttt cgggatcttc atattccaat aaacgcttag ttttaaattg
     9781 tgcgtccttt tctaatcgat agatttttgc attttgaatg accaatgagt caatttcttc
     9841 aagaaaatcc tgatttgaat caaaccaaaa aatgattttc ctacatccat aattaggaaa
     9901 acgttgattt aatttttctt caatttgttg cgtatttata tctgtcatca atctcaccta
     9961 atcgttctct ttctataccc cttatattga cataatttgg ataatttcgc acgcaattta
    10021 agaataaaaa agaaagttgt tagaaaacaa ctttcttaga taactatttt tttggttctt
    10081 tgaccccttt tgcaaaaatt tggaatttct cgccggtttt aggatcacgc tgcaacttat
    10141 cataattgac aattacacca tcatctaagt ctaattcaat tcgttttaat gctaagtgac
    10201 ccaacttctc gtcatactca cgaacttctt ttaattgctt ctgaatcttt acaatttctt
    10261 ttgtcattgc atttctttct tttgggttat cacttgttga aatttgttgc tgacgcaatt
    10321 cgattcgatt gtcatacgct ttttgtagtt cgtgtaggta ttcagtacgt acttttccta
    10381 attgatctgg tgtatagcga tgcaagtaca ttaacgcttt aaagccattt tgttttcctg
    10441 agtccaattg ccaataaatt ggtcgattgg agtatgtttt taaatggtct ttgtaaaaat
    10501 cttgtacgaa gtaacgacga ataatttctc gacttgtccc tttgccacct aaagtgtcag
    10561 cgataaactg gagattttct tcaagggttt ctttaccaaa agtaatctca acgaaatcaa
    10621 tgaaacgatt gacaatgtca atttggctat ccttgaaata ctcatcatcg gtgatgagta
    10681 aaacattatc agtatcaggt ttaaattttt gatagcggga aggatcccat tcgccgccag
    10741 cgaagatgag accatcttcg tctagggagt aacgaccgaa gatacaacct attgaataag
    10801 aaataaaatc cttaatttcc ctcgccttat caggtaacct tgcagagata tattcatctt
    10861 gaacctcttt tgttaattct ttttcaagag aataatgatt aatgaatatg tcgttcagtt
    10921 cctcttcatt cgattttaga gtattaaatt gattaaccaa ctgcctactt agacagttaa
    10981 atgcctctga gagatctttt ttaaacatat aacttaacaa aatgtttctt ctaaactcgt
    11041 atgacgtttc aagagcatta tagtcttttc tagctatatc gatattttca tccacttttt
    11101 ctcttatttt ttttaatttt ctctcagatg taactttaag aaaaggtagt tttcccattg
    11161 gtccttcatg gtaatccaag gtgggagaaa taaaagttaa aaagtgatta actaatttag
    11221 agtttacata gccaaatatt tcttcaaagt cagttatatc ctttgagaac agaacagatc
    11281 ctttagtttc aaaaattgcc tgattctgca atctaaaaga tacttgtcca gatgaaagtg
    11341 tcgaccaagt aattcccttt ttaaaatagt aattaagatt ttgtggtcta gattttaact
    11401 tcccattttc attaacataa ttttttattt catctccatc attctcccag ttaaccacgt
    11461 attcattgtt tccataccat tttctaaaac gaccaccttt agttgtagga aaccattttt
    11521 ttctagactt tttagcgtcc tctaatgttt tagcatcaaa ctttattttg ttaaattcta
    11581 cttcaaacca ataccgcaaa aatttattgt tatttccggt tgccaaccct tgttttgcat
    11641 ctgcaacatc gttagttaat tttccttgag caaaatcagt gattacgtta tcgcttgccc
    11701 agtaagcaat cggcattcct gggatttttt tgaagttgtc ttgattagta ctataaaaat
    11761 aatcgcattc accatgctga tgaattgctt ctaacgtttt aattttttga acttccattc
    11821 cacctttaaa atctgataat tttatatagc ttccatcttg ttcatttgaa ttttttatcg
    11881 taaacgtaca aattggaacg gttgcttctt caaaagctga atactccatt tgaatcaaac
    11941 tagaaatgtt tctttcatta attaaaaatg agcgtaactt ttcatagcta gaaatgaaca
    12001 tccaaacaaa tggcgtcata aaggaagcat atccattatc aatcagcatt tggctattca
    12061 atttaataaa cacagaaaat agatcttttt tgacatcaga atagttatca tttacatatt
    12121 tttttaattt cttatccatt ttattaagat aaggcggatt agtgactacc gctacatatt
    12181 tatcaactag catttcagcc acgtcaagaa tgtgcaagta tttttcttgt atttccttca
    12241 tcccataaag aatatccata ttcgtaaatt cgttgaattt tgatatatac tctcttactt
    12301 ttcctaagta ttctttagtt attgcctgat tatttttatc gactaactta atgattgatc
    12361 caagttcttt cccattttca aattgatcaa aaattaactc tttgtattca ttccagcgtt
    12421 cactatactt atcattgttc aattcactaa tacgctcttg atactcgtct gtcactttag
    12481 gagaatcaat aaactctctt aaattcggcg atagactttt tttcaaaaaa agacgatcgt
    12541 attgacgccc tttcatcata attgcaaaat aagacaattg atacgctctt ttatcgattt
    12601 caagaccata taaattgtat tcaacgattg cacgtgcagc atcacgctca gaatatccta
    12661 acgtaagata aatttccatc aatacatcga aggcgtaaac taaaatatgc ccagacccca
    12721 ttgcgttatc gataattttt agttcttctg gttttaaatt acttacagat gttgcttgtt
    12781 caaaagcata tttatctgga attaagtatt tcaacttatt tcttaattca ctattgggat
    12841 aatgatctaa ccaatacttt cctaaactat tgtcaaccat ataacgaacg acccaatctg
    12901 tggtaaatag ttgagttgct gccggaatat cttgtttttt gacaatccct ttataaatat
    12961 tcaccacttg attatgtggc tcactattat agtactgata caaccaccca ataatctcta
    13021 cctgtccacc agcttgaaca tagaaatcat cctctggaat acttaaaaga tcagcgatta
    13081 caccatcttt tttaatataa gaagctgtaa acaaaagttc tgtataatct tctacttctt
    13141 caaatagctc aggtaagtaa tcattcaatt gattacagat ttttataaaa atcaattgat
    13201 acagcttgtc cattgcttct gctgaattat ctgttttcca agttctgact tgttgctgtt
    13261 cattactaga taattcttca aagatatcgg catcataaat atctgtaata atatctggct
    13321 caattttccc acttatttca ctagataata cacgatacgt gtcaggtaaa taatcattta
    13381 cctccataaa ccgaatggca atcaatcggt taaaccacgt ataagcaacc tcttcgatta
    13441 actcttgaaa agcttctgaa taagaagtga cagaagcacg ttgttttagt tctgcaatca
    13501 atttgtttct ttgcgctatt tcttttcctt gaattcttat tcctgatttt tcgatttctt
    13561 gtaaattgct atcaattttc ttcacatctt caatttttga ttcatcaatt cctaaacgtt
    13621 ttgctttagt cattacttca tcaattaatt ttctacgggc attgaccgcg aaattcttta
    13681 tcgctttctt atccattttt ctttcccttc tatatgtaac ctttgaaaag acaggcacct
    13741 ttttatgcct gtcttttcac tagaattgaa tcgtgatttc tgtattttct tgaagttgtt
    13801 ccttcagttt ttgattaatt tcagctgtta actcatcaat atctgctgct gacttaatag
    13861 ttttaacatg aacgttaagt gttctcattg gtactgtttt ctgttgaata gtaggtttta
    13921 ctatcgctat ttcttcagct tttgctgcag agtctatttc atctttttca gtttcacgaa
    13981 tcaagtttgc tgcttcaatt tgtttacgtg cttgttcttc ttgacgtctg cggttttctt
    14041 cattatcgaa acgttgatta aagatcaaat actgttctgc tacgtttaat gataagaaaa
    14101 caagatcatc tatagtattc gcttgttcaa tttttgcttt cagttcttga aattcttggt
    14161 caactattgg tacaaattga tccttaaaat cttttccttc taataattta aatgttgctt
    14221 gtttttcttc cgttaattta tttaatattt cctgactttc caactctaac agttgattat
    14281 attgttcaat aaattcttga atcaactcat ttagtttcat catatctgaa taaggatcag
    14341 cctttttcaa aatggttttc atctcatgaa tcaattgatc aatttcttgg ctagaagttt
    14401 gacgccgact tttttcgatt aatctaattt tatcttgtgc attctcccag atttttttct
    14461 gagtactatt ttgcttgtgg aaatttttaa ttttatcata tgtttcagcc cagtcgaata
    14521 attcatcgcg atattttttg attgtataaa aaaagtcttc aacatctgac gtttgaacaa
    14581 ttacttcaag atattttttt atttgctcat attcataagc actagggtag gtataagtgt
    14641 tttgtaaatc aaaataagag cgtaaattgt ctaattgacc ttttaaatat ttcttgaaat
    14701 tttccattgt atgttcattg ttattttcat caacaatatt acgactttga aacagttctc
    14761 taccgatttc gtcaaccaat ttttttgctt ttggcgaaat caccgcgctt ggttcaattt
    14821 ttatcttttc actttctcgt cgtttttgaa taatcattac aatactattt ggatcatcca
    14881 tccgtgaaat ttgtacattg ttataaagta aatcaatttt accagacatg aaaagtttag
    14941 cgatgcacca ttccacatca agttctgtga atccatacgg ttgttttgtt tcaaaacgat
    15001 cacgaattgt tttcatcgta atcgttaggt gatttctctt ttgatcattg ataaaattga
    15061 aaatttcctg aactgcttgc tcatttgatg gatcttcaat aatttcattt ttgctttctt
    15121 tcactaaata taagatatct tctttttctt taggcgcatc aatttggttt aatttatggt
    15181 atacttcttc agccattttc tgaagtgctg attggaattt tgctttaaag tcggagcctg
    15241 tcagttgaat ttctgcaccg ttatagaaaa attttgcaga tttcaacagt tcatttaatt
    15301 gatcgttgat acttgcactt agttcctgta attcaccaga tttttccgaa cgaatcgcgt
    15361 caaagttctt tactacactc gaataagtcg ttgttaaata aagattcatt ctagcataca
    15421 ttgctatatc atctttaaat tctttatctg atggcaattc tacgaataat aagttacggg
    15481 aactcttaag cgcaagttct gcctcttgac ctgctaaatc agagtatggt gaaataaaat
    15541 taactgtcat ttcagcattt ccagcttgct tgtatggacg ttgatctaac acttgtgcat
    15601 aagtaaacgt ataccggttt ttaaatttat ttccacctga catgcctaat ttttcatagg
    15661 caaaactatt tcggtctaga acgccgtcaa aaattcgttt agatagttct acgatcaaat
    15721 cactatctgt aatattttga tttttaatct cacgagtaat ttcctgttct tcatcagttt
    15781 ggaaaatata actatctaag ttcttccgca caaagttttc cttaattaaa aggtccaatg
    15841 cttcttctac tcgtttttta gtttccaggc ggtcttcgtt gatatttctt atcatcaagc
    15901 tcgtaatatt ttcaacattt ggtttgatgg cttgatcaac atatttaatc atgaataaaa
    15961 ttcgtaagac atttacgtta aagttatcta tatcagttgg tctttgaata tgagaattat
    16021 ttgtagcttg tgtaatcact cggctaattg ctgtatcaat ccaacgttct aacgagtaat
    16081 aaaactgatc taaagaaacc aaactggttg tatcattttc cttatttcgt tcagcggctt
    16141 ctttaaacat tgctagtaat gaacgttcac cagacgataa gtttgatcct tgatagctgt
    16201 gattacgaat ctcggttaga atttgcccca ataaattgaa ttgataggga ataaatggat
    16261 aaacttctgc gtaattttca gcatcttgga agagctttaa ttcaggacta tcaacaaaga
    16321 gtaatttatt tttcaattct gtttgaacag tttcaaattg taaggctaaa gaatcattgg
    16381 caaactcttt tttcttcaaa atacgttctt taattacttc atcggcattt gctgaagata
    16441 aactcacagt tgtggcaaaa cgatccttga tcttagaaaa atcattatca gaattgttat
    16501 caaagtttgt tacgtgatca atcgcttcct gacttgtcac taatacccaa gccttacctt
    16561 cacaaattcg acccaaatct tccacaacgg tttgtaactg caaagtcaac tcacgattac
    16621 cgccgatata ttgtccgacc tcatcgacta gaaaggcaat atgatggtct gcttctttgg
    16681 aatcaagata gcttttaact agatttgcaa agtcatcaat gcttattaaa taatttcctg
    16741 atgctttttc taaaaaatta tcaatagcgg cttcactcat aaaatcgatt ttaagtgccg
    16801 cttctttgat atcatcttgg atccaatcaa actcatggcg ctcttcttgc cattttgccc
    16861 cattaatctc ttcaaaagca gctttgaatg catcaaactt ttcttcttta attaaccgac
    16921 gttcaaaatc tgctagatgt ggaatcgtgc ttaatccttg catttcgtta aataccttta
    16981 aaaaaacatt cacgattccg tttttgtctt ttttaccacc tgcatcactt tttgaatcaa
    17041 tattaaacag aataacatct gtatttattt gtgagcaccg ttccatcgtt gccaaagtca
    17101 gtggatcttt gattttccca tcttctttga agtaatcaat ggctttcttg tcatttattt
    17161 ctcgatttgc gagcaagtaa gaaataattt ttaagaaatg agatttacct gaaccaaaga
    17221 acccctgaat ccaaacaccc attttatctg ttggtgtatt gatactccgt tggtaggcat
    17281 caaaaattgt tttaaagtgt ttttgtagtt ctttcgtaac cacgtattct tctaattctt
    17341 gttgaacatt ttcatccttt gcttgaccga ccttaataac tccttgaata tttcggtcaa
    17401 ttggttttgc aaaaatatct ttaattagca tttaatctcc taccttccat cccaactaaa
    17461 ttctaaatgc tcgataataa ttatcatctt tattggtatt aagaattgtc aaacttaatt
    17521 catcatattc ccctggatac atcatcacaa ctggtacgcg atcaaatacc tgatgcattt
    17581 tatttaaaat agagtgcgct ctaattaatg gatataattc gccaattcct gtcaggaaca
    17641 caactgattt ttctggtgta tgggcatcca catattcaaa aaatacattt tcttcctcag
    17701 gtaaatccat tttcaataaa cgaccaatgc tttccactaa tttttgtaaa ccacgtcgtt
    17761 cttccatctt aatcgctttt tcagtgaaac cgcgtttttc tagatactct aacataatca
    17821 catagagatt ataaactttc acttcataat tatcaccaac agatgttcca gatatatcat
    17881 taatataatc tcttacaatt aactcgtctt tcggatcgta gtcaaagata taatagccta
    17941 cttcattacc taaaccacga ttcaataaaa aatcttcttt ttgaattact tcattcaact
    18001 tttccaaatg ctcttgaatc gataatgaca ttataaaatt cctcccagtg ctagtacaat
    18061 atctttacga ttcttgtcaa ctaaccattg aatcaatcgt ttatctaata atatcggagt
    18121 cattcgtcca tattcttttt cccctctgac taatcctgtt tcaattagat tcgtttgata
    18181 agcggcaata agccgtttaa tcgtataatc tttccatttt gaaatatctt ctgaacttct
    18241 ccgcttctct gtaaaaaaag aacggtaatc aactggtgtt aattgcaaat ttcctttacg
    18301 tagattttca gcatatacct cagtcataaa ttcattaaaa agatcatcaa tcataaggat
    18361 ggaaatcaaa ttcactaact tttgattatc tatatttaac tgattgaaaa gttgcaatcc
    18421 agcttcatcc agttgactaa ctctcctttt cacgttctga tagatttctt tttgacggtt
    18481 actgctcact gcaaaaaaaa tattttcatg ttcatttttc tctttaattt ctatatctgt
    18541 atatccttct agtcgtaatt gaatatataa cttgaactct gaaaaccaaa agcgtttaga
    18601 aactagacca gcagaatatt ttttattcaa tagattgcct cctaatcatc tataattcaa
    18661 taagtaaaca agtgtttttt gctcatcgtt agcattcatt ttttagtatc tactatacaa
    18721 tacatattta ttata
//
LOCUS       DAZKOU010000058        18504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000058 DAZKOU010000000
VERSION     DAZKOU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.059"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG827_001905"
     CDS             99..746
                     /locus_tag="ACG827_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0484037.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG827_001906"
     CDS             1139..3784
                     /locus_tag="ACG827_001906"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0484038.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG827_001907"
     CDS             4094..5407
                     /locus_tag="ACG827_001907"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0484039.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG827_001908"
     CDS             5397..6050
                     /locus_tag="ACG827_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0484040.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG827_001909"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG827_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0484041.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG827_001910"
     CDS             6947..7147
                     /locus_tag="ACG827_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0484042.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG827_001911"
     CDS             complement(7401..9080)
                     /locus_tag="ACG827_001911"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0484043.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG827_001912"
     CDS             complement(9082..9294)
                     /locus_tag="ACG827_001912"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0484044.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG827_001913"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG827_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0484045.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG827_001914"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG827_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0484046.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACG827_001915"
     CDS             13269..13466
                     /locus_tag="ACG827_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484047.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACG827_001916"
     CDS             13619..14104
                     /locus_tag="ACG827_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0484048.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG827_001917"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG827_001917"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0484049.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACG827_001918"
     CDS             14981..15508
                     /locus_tag="ACG827_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484050.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACG827_001919"
     CDS             15599..16336
                     /locus_tag="ACG827_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0484051.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACG827_001920"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACG827_001920"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0484052.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACG827_001921"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACG827_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0484053.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3199 c         4022 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgt
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAZKOU010000059        17374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000059 DAZKOU010000000
VERSION     DAZKOU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.060"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..615)
                     /locus_tag="ACG827_001922"
     CDS             complement(70..615)
                     /locus_tag="ACG827_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0484054.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(627..1319)
                     /locus_tag="ACG827_001923"
     CDS             complement(627..1319)
                     /locus_tag="ACG827_001923"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0484055.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1338..1622)
                     /gene="macP"
                     /locus_tag="ACG827_001924"
     CDS             complement(1338..1622)
                     /gene="macP"
                     /locus_tag="ACG827_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0484056.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1635..2198)
                     /locus_tag="ACG827_001925"
     CDS             complement(1635..2198)
                     /locus_tag="ACG827_001925"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0484057.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2426..3088
                     /locus_tag="ACG827_001926"
     CDS             2426..3088
                     /locus_tag="ACG827_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0484058.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3212..4408
                     /locus_tag="ACG827_001927"
     CDS             3212..4408
                     /locus_tag="ACG827_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0484059.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4509..5885)
                     /locus_tag="ACG827_001928"
     CDS             complement(4509..5885)
                     /locus_tag="ACG827_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0484060.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5922..6788)
                     /locus_tag="ACG827_001929"
     CDS             complement(5922..6788)
                     /locus_tag="ACG827_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0484061.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6877..7902)
                     /locus_tag="ACG827_001930"
     CDS             complement(6877..7902)
                     /locus_tag="ACG827_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0484062.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7920..8594)
                     /locus_tag="ACG827_001931"
     CDS             complement(7920..8594)
                     /locus_tag="ACG827_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0484063.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8778..9671)
                     /locus_tag="ACG827_001932"
     CDS             complement(8778..9671)
                     /locus_tag="ACG827_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0484064.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9745..11442)
                     /locus_tag="ACG827_001933"
     CDS             complement(9745..11442)
                     /locus_tag="ACG827_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0484065.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11435..12748)
                     /locus_tag="ACG827_001934"
     CDS             complement(11435..12748)
                     /locus_tag="ACG827_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0484066.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12748..16689)
                     /locus_tag="ACG827_001935"
     CDS             complement(12748..16689)
                     /locus_tag="ACG827_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0484067.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16798..17256)
                     /locus_tag="ACG827_001936"
     CDS             complement(16798..17256)
                     /locus_tag="ACG827_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0484068.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4864 a         3900 c         3018 g         5592 t
ORIGIN      
        1 aaagtcgttt gttttatgct cttcttttat tattttgtgc tttttatttt atcaaatcac
       61 tttgtttttt tataaaattt ctgcgcctag tgtggactca aaatgtctca atgcccattc
      121 atgtccaacg ggatcgaaac ttgccacgga atctttgaaa atatggagtt tgtatcctag
      181 attatacgca tcgactgctg tatgcaacac acagatatct gtacatacgc cggtcaaata
      241 aatatctgtg atctggcgtt ctcgcaaacg gatgtctaga tctgttccac taaaagcgga
      301 ataatgccgt ttgtctatcc aataaacatt ttcctcttgc tcatagtctt ggtataaact
      361 ttttaaagag ccgtacaatt cccgtccacc cgttcctatc acattatgcg gaggaaataa
      421 gcggttctcc ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa
      481 atccttttga tcaataaact tctttgtatg agcagtcagc tttttttcaa ttacttgtcc
      541 tgctgtccca gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga
      601 gatcaatgcg ttcatcttag tcctccttat acgagatgtt tgacaaattc gatcaccatt
      661 tctgctgatc gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt cgccgagtga
      721 tctgctgtgt cgctcattgc acgtacgatc aaaaatggga tattgaactg acgagctgtt
      781 tgtccaacag cggctccttc catttcgcaa gctaatgctt cagggaaatt cgttaagatt
      841 tctttgattt tatctggcga gtcgacaaat gtatcacctg taacgatcaa tccttctttt
      901 gcgttcaaat tcgttgcttt tgctgcttta accatttcac tgcgcaaata tgtgcttgct
      961 tcataatata gaggcattcc tggtaattgt cctgggttgt agccaaatcc tgttgcatct
     1021 gcatcaaaat aagctacttt gtctgatata acgatgtcac ctacttgtaa gccttcaccg
     1081 attccgccag ctgaacctgt attgatcacc atgttcacgc cgtattgttg gatcaacaga
     1141 cttgtggtaa tagatgctaa gacttttcct ataccggaac gtacgacgat cacttcgtgg
     1201 ttacctaatg aacctgaaat aaataaagcg cctgcgcgtt cccatgataa tggttcgctt
     1261 aggttctctc tcaaaatttt tacttcttct tccattgcgc cgataatacc gattttcatc
     1321 tttttaattc cttctttcta tataaatgcg attgctaaaa aaaccacaca taacattagg
     1381 ataacaataa taagagattt catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg
     1441 ctgatccttg ttttcgtgat tggtttattc tttttgcttt ctttacgata aaagctggca
     1501 atcttttttt cttcttgctg ataagctgtt tctgcttttc gctgtttttt taatgactcg
     1561 cttgcttgtg cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc
     1621 ataagccgat ccctctactt tcctttggtc aatagttccc agtattgaat cgcataaatc
     1681 gtctttgcat cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc aagagttaga
     1741 tgatacaact ccagtacttc gtcttcatct tgtgctaaag gattctcgac cttctctacc
     1801 ccttgtgcat gataaatatg aaggacttca tttgcaaaac ctggtgaaag atacatcgaa
     1861 gtaagatggc tcagcgactt cgcacgatag ccagtttctt cttctaattc tctagctgct
     1921 gtcatttcgg gattttgtcc ttctcctgga tcgatttttc cagctggaat ctccaaaatc
     1981 actttttcta aaggttttcg aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa
     2041 gcaatgatcc cgacaccgcc aggatgaaat accagttctc tcttcgaagt tcctccatct
     2101 ggcaaacgaa cttcgtctac agcaacatca ataatttttc cgctatagat ttctttgcga
     2161 ctgattgttt tttcttcaaa ttgctcattg ttcagcatat cttctcctgc tttctttgta
     2221 atcataactg cttttttttc atttttcaat ttttctcaaa aactccttat tagtttacca
     2281 tagataaacc ttagattttc tctaaatata gaaaatcctt ctttagtccg atactctttc
     2341 tagtagtccg tatgctaaaa taagtatgta atcgaataac gaaaagaatt caatactcaa
     2401 cgataaatga catggagggt ttattatgat aaaacgaaat tggccgtggc ttctagcatt
     2461 ggtcttgcta tttctctttg tccgaaacaa tagtttttct tttgttctag gacttttatt
     2521 gataggtgta gttttagttt tgtggggact ctttggttcg cgcaggaaaa agggaaaaca
     2581 agaagaaatt cctgcactga ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac
     2641 agcgaatgaa atttcatttt ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca
     2701 attgcaaatg aatatgcagc agacagccaa acttaaagca atcgatctcc gccatgatcc
     2761 agtcaaagca gcgaaagctt tattcaaaga attggtgaaa gaaccaaacc gactgcatga
     2821 agcaagtcag tttctttata cgcatctgcc gaatctagtt gatttgacaa ataagtataa
     2881 tgagatcaat gaccatgaga tcaaaaacaa acagacctat gaaaaactag aagaaagcgc
     2941 gcagatcatc gaccagctgt cagctttaat cgcacaagat tatcaaaaat tcgttgctga
     3001 ggatctagat gatatcgacg tagagatatc tgttgcgaaa cagagcttaa agcgtgataa
     3061 taaatatgaa ccagaacaaa aggattaact cttttgttca taaaagaaga ctcggaacat
     3121 tcagatgatg tctggctgtt tcgagtactt tttctaaagt agagacattc atgtaagaat
     3181 cgagaacaga ttcaacaagg aggaattttt tatggatcat aaaccgactg aaacaaaaga
     3241 tgttacacca gttaacgata cacttgaaga tttattgaac aacccgtttt caactccaac
     3301 agatacgctg accacttcac aacaagcgga gattgatcag ctccaagagc aacagacagc
     3361 tgctcgttta attgacaaac taccggcgga acgccaagaa caagctaaac agctagcagc
     3421 aaaaatcgat gctaatgatg cacagtctgt catcagctac ggatcggctg cccaagcaaa
     3481 attaagtgaa ttctcacaat cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt
     3541 aggagattcc ttaactgaac tgatgtatcg tctccaagaa gccaatcctg acgaactgcg
     3601 tgccggagaa gggaacatct tccagcgtgt cttcgggaag gtcaaacaat cgatctatga
     3661 agtgacagca aaatatcaaa agattggtgc acaaattgac aagatttcag tgaaattaga
     3721 taaagaaaaa gacggtctgc taaaagataa tctgatgtta gaacagcttt accagaaaaa
     3781 caaagactac tttgatgcac tgaatattta tatcgctgct ggagaattga aaatggaaga
     3841 acttcaaaca acgatcattc cagaagcgat gaaaagagca gaagaaactg gcgatcagat
     3901 ggatgttcag attgccaatg attacacgca attcctagat cgtctggata aacgtacgca
     3961 tgatcttcgc ttagctagac agatcacgat ccagcaagca ccacaaatcc gtttgatcca
     4021 aaacacaaat caggccttgg ctgaaaagat ccaagcatct atcgctacag ccattccgct
     4081 gtggaaaaac caagtcgtga tcgcattgac cttgcttcgt caaaaagatg cagtaactgc
     4141 tcaacgccaa gtctctgaaa caacaaacga cttattgaag aaaaattcag aaatgctgaa
     4201 aatctcagcc atcgaaacag cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct
     4261 tcaaaccact caaaatgatt tgatcgaaac aatccaagag acattgcgta tccaaaaaga
     4321 aggaaaagaa aaacgtcgtc atgcagaagt agagcttgga cacatggaag aagatctgaa
     4381 acaaaaactg ttggatttaa cccaatagat cacctttcgc ttgttactaa aacaaacgcc
     4441 caacaacagt ttattacaac tgttgatggg cgtttttctt atctttcttc taacttctcc
     4501 attggagtct atttctcatt catcttgctg tgtccatagc catagccaaa ataaatgatc
     4561 agtccaataa ccattacgat accgaacacg atccatgtca cagtttgcag acgaagcatc
     4621 aatacaatac aagtcaagat ggagacgatt ggcaatagcg gaacgaatgg cactttgaat
     4681 tctccagctt ttggtttgcc aaacattttt ctcaacttca gtaagcctag actaacaagc
     4741 atcaaatagg cgagcgccat gatattcgtt agttccgcta atacattcaa tgggaaaaat
     4801 ccggcaaaga atacagctgc cacaccagcg gcataagtcg cttttttcgg tgtacgatgc
     4861 ttttgatcga tttctgtcat aaacttagga agtaagccat ccttgctgac agcaaacagt
     4921 aaacgagcaa gtgaatacat catcgagatc gttaccgtca gtagcgttaa aattgcccct
     4981 acagcgatca ccgcgcccac tgttccatgt cctacatacc tcattgcaaa agccactggg
     5041 tcattgactc ctagttcagt atacggaacg attcctgtca agatcagcgt cacgatgatg
     5101 taaaggacag tagcaatcag tatcgaacca atgattcctc ttggcacatc tttttgcggg
     5161 tttttgactt cctcggctgc cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca
     5221 agagctgcac catctagcac accggaaaaa ccaaaaggca tgaatggctg ccagttatca
     5281 ggtttaacga aaaatattcc tacaatgata aacaacgcga tgataccaaa tttgacatat
     5341 accatggcat tattcaaacg taatgctttc ttggcttcaa gcgacacgat gtaagtgacg
     5401 aatatcacta cgagcaccgc aatcaagtca atgtatgtac cattttccgg attgtacccg
     5461 ccagttaatg cttgtggaag ttcggcccct aaacttttca aaaaaccttg catatagcca
     5521 gaccatccgg aagcaacaga ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct
     5581 gtcagccagg cagcaaattc accaaataca acatacagat aggcatatgg cccaccaatc
     5641 actggcactc gcgaagcaaa ttctgcgaaa cttagtccag acaaaatgac aacgattgca
     5701 gctaagacaa atgaaagtga cagtgctgga ccagcatttt gattggccgc aacccctgtc
     5761 accacaaata ttcccgtacc gatgatcgca ccaattccaa gcatgatcaa atcagacgtt
     5821 tttagttctt ttttcatctc actcgtatga ttgagattga tctctttctt acgaaacaaa
     5881 tccattgaag ttcctcccca ttttaggttg gaaaacagcg attaaactgt tttcatatac
     5941 cctaaaagga taccacctaa aatgactaaa aggcaaccaa caataacata aatgagctct
     6001 tttttcgttt ttctttcgcc aaggatgaaa atcccaccta aagtagagat aatgatcccc
     6061 atttgtgaaa gtgagaagcc aacagctaaa ccaagtgatt tgactgttag aagcatgcag
     6121 atgtttccta agccccataa taatccacag atcatgtttt tccacacaaa ctgatcgact
     6181 ttaactttac ggaaagcaaa gaagcttgcg cctaagatca ttccgatact ttgaggtaag
     6241 ataattgaca tcgcatccaa attcgcccag ttgacgatga tggtatatac accataacca
     6301 atcgtagaga agatcaatgc tctaaaaccc ttaccaaaat caagcatttt attatctgta
     6361 tctatttttc cttcgtcatc ttgtcgggca gtcaaatacg caccgcttac aagaagagct
     6421 aaagcaataa taccgtatac ataatctttc gtttgtgtcc attcatggaa gaaaacggct
     6481 cccgcaatcg tattcaaaat taattgaaaa ccagtagaca atggtagccc tacagaaaca
     6541 cccatgtact tcattccatg gaactgtcca ttttgtccaa cactccaagc taagccggat
     6601 aaaaatccga tcaagaaaat ccaaggggtg attacaggac taacaacgaa aaagacaact
     6661 aaagaaaaca gaaatgcacc gatcgtcatt cctagtgttt gttgattggc atcgccacca
     6721 attttaccac ttactaaacc aatactaccc catgccaaca tagggactaa tgctattaaa
     6781 atttccataa ccaatctcct tttatttcac atgataagaa acttatcagc gaaaacgtta
     6841 acaacacaag cacttttttt cgtttctaac gattttctaa cgtttttcat tataaaaatt
     6901 taagattggg aaaaataaag tcattgttgt cccttttcct tcagttgatt cagccgtcaa
     6961 atggatacct aatttttcgg ccagattttt tgctaaataa agtcctaatc ctgttgaatg
     7021 tgttttgcta agtcgaccat ttctcccagt aaatccctta tcaaagatcc gtttcaagtc
     7081 ttccgctggg ataccgatcc ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt
     7141 ttctatgctc acaatgatgt ttccgtgatc cggcgtatat ttgatggcat tactaagcaa
     7201 ttggttgaag ataaaagcga tccatttagc atcggtcaaa acagtatgat cctcaccaac
     7261 gatctcgtag cgaatatttt tttggataaa ataatttgcc tgactgcgta tcacaggctg
     7321 aatgattcct tttagtccat attcttggat gaggtagtcc cgcgagaaac tgtcgagtct
     7381 tgcataatac aaaacttgtt ctacgtattc atcgattttt gataattcat tttctagtaa
     7441 tgtatacttt tgatcgtcaa tatcgaactc gatcgaacga actagcaatt tagaagcgga
     7501 aagtggcact ttgatttcat ggacccatga gtcaatataa tctttttggt cttgttgatt
     7561 actgattgct gattgcatta cttcttggtg ttcacggatc agctgattga tatattcttc
     7621 taccagtttt tcttcttctg tcctcccacc actcaaataa ttttgcaaag gggaagtttt
     7681 ttgtatgtcg gctaattttc cccaccatct tctcttgctc aagtaaccac ccagcagata
     7741 aaaaatcaga aagacgccct caatcaaagc taaataagca attgtgctca aatcaacttt
     7801 gatgtttggc gccagccaca tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa
     7861 gatcaatgtc cactgatctt tcaaatattt aaaaaaattc atcttaaccg ctcatccttt
     7921 cagggaacga tatatccctg tcctactttt gtttcgatat atccttggat acctgcctgt
     7981 tcgatttttc ggcgtaaccg attgatgttg actgtcaatg tattgtcatc tacaaatcgt
     8041 tcgtcatccc acagtgctcg cagcaatttt tcccgactta agatctttcc attgtgcttc
     8101 atcaaaataa ataatagacg atattcattt ttgctcaaat caatgatctc tccgttgatt
     8161 tccatacttc cgttgtcaac attcaacgta atgccattgt ggctcatcat ttcactagat
     8221 aattctgtgt agttgtaaga acggcgaagt aatgcattga tttttgcaat cagtacatcg
     8281 atttggaaat gttttgtcac aaaatcatct gcccccatgt tcatagccat gatcatatcc
     8341 atattcgtat tcctgctgga gataaaaatg atcggtactt tcgatacttc acgtattttt
     8401 tgacaccaat aatagccatc caaaacggga agattaatat ctagtaatac aagctgtggt
     8461 tcttctcttt caaagtctga gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat
     8521 tgccatttct caagttcctc catgatcaat tgtcgaatga tctcttcgtc ttcgactacc
     8581 atgatttttg ccataaaaaa tccctccatc tttcttcttg ctactacaat atcaaagaag
     8641 cccttgtgaa acaagttggc ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac
     8701 tggaaatatg cgatttaatc gcgtttgtct cttccaagca gcttaaattt attatgttat
     8761 tcttaataat tggtctatta tctgttattt ttctttcgtt gtttttgtct tttaaatttt
     8821 cgataacacc aataaccgaa aagcgttagc actagcaata tacctagaag gagtataagt
     8881 aatcgataaa gatggtattt ttttgtctgt tctatctttg aagagacttc ttctgtaaag
     8941 ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg
     9001 ataagtgtga ctagatcctg cttttctcta attgctaatg tatttgtttt tgttggaagg
     9061 acaacaattt tctcaacgac ttcataagcg agcatccgac catacacttc aagatagaat
     9121 cggtcatctt gctccatttc ttcaagatcc gtaaagagtt ttttctcagc aataccgcta
     9181 tgggcggtaa tcacggaatg tgtactctct cctccagttg gaaaagaggt gccttgcagg
     9241 actgtcgctc ctctatctaa caaaagatca ttcgtttcat caaataaagg caagctaaca
     9301 ttgattttcg gaatatagat ggctccgatc atatgttgtt cgtaatattc ttttcctgga
     9361 ttttggacat ttctaaccgc ttgctcaaaa ggatctttga cttgtcccat tcctgggatt
     9421 actgatgtat gagcaagcgt ctgatttttc ttttcatgca ctgctaattg ttgctcatgt
     9481 ttttcttctt tatcgcgttc aagctgcttt tgataattct cgatccttct ttgatccaaa
     9541 taattgctga gagcatctgc taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac
     9601 atcactgcca taaataattg taataaaaga ctgatttggg tttgaggtct attacctatt
     9661 ttctttttca ttttcttcac cttcttattt aaaaaaactg cagcaacctt tataaaaccg
     9721 attgctgcag cttatttttg ttgcttatac ttcagacttc attttagatt tacggtacca
     9781 gctgtacgct ccaatcatca aacttattcc gattagtaag aatgccaaga gcccgttccc
     9841 accagtagca ggaagtgacc cgcgtttcat attcggtact ctttctggcc ctggattttc
     9901 tactcctacg atctgaattt cttcattata gcttccataa gagatgataa attcagtaaa
     9961 ggctgcctca cctgttggaa gtacataacc ttctggcgcc atggtctctt tcatttgata
    10021 ggttccataa tctaaccctt gaacctcaaa gtatccattt ccatctgcga caaattctgt
    10081 tgcttgtccg cttgttgccc actcaattct atctgctcct gattcataaa cagctaaagg
    10141 cgactcccct ttataaaata ttgcgtattc agaacggtct tcattccaca attcgaatct
    10201 tgcatcttta agcaattcac tgcttgatgc atcatatttt tcaaactttc ttccacctgt
    10261 tgtgactttc tctggcggct ctactccttc ttcataatca aattcatcat ttcgtccgat
    10321 ttcaataacc gctgtgttgt tatggaaatc atccgcttct agttctgtgg aaaccttcaa
    10381 attatacgtg atcgttaaat ctcctccacg tgcatctgct aatctaccta aagtatctac
    10441 tttggctact ccattttctt ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc
    10501 tactatataa tcttgtcctt ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg
    10561 atcataataa gttagaccaa ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc
    10621 gcttccgttt tcaaggacag aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga
    10681 agccgttttt cctctttcta gatcaatata agaaatcgtt tcttgttctc cagcttcatt
    10741 aataatcgtt acttgtctaa ggtcgctttc ctctacattc atctgtttat ctgcttctct
    10801 gatttcgttt ttcggataca aatgcacatc tgtattgtat gtgtctttcc atgtagaacc
    10861 atcccacacg ccatccacca tatcaggcat catcactggc atcataacaa ccattggaga
    10921 cgcgattctg gaaatatgag ctggcgaata agtttctaag aaaagataga ctttatctct
    10981 agaagaaggt gcttcttccg tcacttggac ggtagagaat accgcttctc cagcttcatt
    11041 cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg
    11101 tttttgaatc cattcaatcg cttcggcaga attaagaccg tcttctcgtt ccggtgttaa
    11161 aggattatct cgaatcaaat cgtaatatac ttccgttacg tcaaacaaat caaaatcgac
    11221 tcctggaata ttttcttcac caaaatccgt agaaaccaat ccgctattcg gtcgttcttc
    11281 cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc
    11341 gacttcttcg gctttgacta gttttccgtt cccgaaaatc cctattcccg cagtaacgag
    11401 taacagtaca ctcattcccg tgacaatcat ttttttattc atttttttct ccccgttctt
    11461 tttttattaa taaaacagca cctaacaata aaagaattcc tagtaaagaa atcaacgttc
    11521 ttgcttcatt cgtttgcggg aatacggacc attttcctga ttgacctgga ttgcttggac
    11581 cactcggctc tttcggtttg tccgttgttt cttttacggt ctcgttataa acacgtggtg
    11641 ttgcggattc ataggcagag actggaactt tcccggattc taattcagcc atctcctgtc
    11701 cgttaactac tacatattgt ggttggcctt ttgcatccac ccaaaattca gggatgatga
    11761 cctcgattct cttacttgct tcatccatct cgtaaccttc cacacctttt aattcttcaa
    11821 aataataggt gccagaaggc aagaaacgtt gtcctgtcgt aacaaggccc tctttatccg
    11881 aaatgaattt gcttacattt ttgtcattca aaggatcatt gtctgctggt ttgatccatt
    11941 gatttttcaa gtcattagca ggagccatat ctaaataata tttcccccct tctatcatct
    12001 gatatagagc aaaaacagca ccttctaatg gttttccttt ttcagttgtt cctctttgac
    12061 gtccatactt gaagaagtat ggatctctta gataaccgac atttttagga tagatatgaa
    12121 tctcttgcag ttcctcggtt gattctgtcg ggtgataaat tggcaaaata gcagcaattg
    12181 ggatagctgt ctgatcgata tcaacatcca atccaacttc tggatcaatc gattcttcga
    12241 aaatcaaata aactgcatcc cgatcttggt ttttcttagg tgctttgata cgtgcaatcc
    12301 cgtctcctaa tccgccaacc gtatctttcc cagttgttac cgaagtccct atttggacaa
    12361 gcctttcttc tgtgattagc tgacggatat tgtttctgtt cgtttcagcg atttgctgga
    12421 cgaattcttt aggagtcatg ttttcagcag cttttttttc atgataataa tcggtcatgt
    12481 cgtaaatggc aaatgtcgct ccatttagtg gcaccgtctg tgaaatgata tctgctgttt
    12541 cccctgcttc ctcagagata gccaagccat cattttcatg gtattcaaat tgatcgttat
    12601 aatcgatatc tcgaatcatc cgtttatgca acacgaattc cacgacatct tcccctgatt
    12661 cttctgccga catcgcatgt ggaagaagga ctgctgcaag cggtagtaaa acaagtaatt
    12721 gaagcatcaa acacaacagc tttttcatta ccttgctccc tcctttttag attgtctgac
    12781 aaacaaacca taggctgctc ctgcaagcgc catgaagaat gcagcaagca ggatatagaa
    12841 taatcgtccg gatcctcctg tagcaggcaa cggattttca ggatcgttcc cgacactaag
    12901 ctgtaatctg attcgatttg tctccaaaag ttcatattct aactcttgct gtagatcagg
    12961 cgtctctgct tcttcgtttg gataatacga aatctcaact tttagtgtac ccgcttcttt
    13021 tcctggaact acccgatcat tttctatgtc aggttcttct cgataactaa tttctaaaat
    13081 aaagtaacct ggaagttttc tatagctgtc aggtgacttc acttccttga tccgatagac
    13141 cccttccaac aagtctgaag cattgaatag ggaagaatcg tttggatctg gtgccaagcc
    13201 tccctcgatg atttcatact ctcctgcact attttgtttc tcaattgaaa attctgcgcc
    13261 ttcaagacgt cggctattca tactgtcaat tttcaaaagt tccaaatcga ttggcgctag
    13321 ttcatcttct aaggttactt gtagtacacc atcgatgatt tcatagcctt ccggcaaggg
    13381 agcagtttcg tcttcgatga ccgtatcttc aatgataaac ttaccttctt cattcagtgc
    13441 aaactggaac acttgatcag atagccgata cccatttggt gcgacagatt ccgagacctc
    13501 atattcacca atagacagat gcagaggttg gtcgtaattt aaatcgacat acttttctac
    13561 tcctacggca cgagaataga agtatccgat cccaggcaaa cgtttcgatt cttctggttc
    13621 taacggatta ccagtgtatg ctcgatacag cgggaaggaa gaatccccca ctttcctgat
    13681 atcgaaacct gcaccatcta atgtatggcc ttcatggtct aattttctaa ctcggatctc
    13741 ccgataatca aacgtattgt aagcagtgaa tgctacccat aaacgatcct cttcatctgt
    13801 tgtatacgtg agatattgcc aattttcttc acctgtttgc ttgagttgga tagtccctat
    13861 agcattatcg taaagtcgca agatccatgt gtcattttca tcaggttctt tgaaaccttc
    13921 tgggttctgg ctctcaatga ttttgtattc tgcatagttg gtagtttctt cactatcctt
    13981 ataaacaaat gaatctggct ctccgattgg atgaataact ttctcaccat ccacgattac
    14041 atatttcccg tcttcatcta ctgaatagaa cgtgagtctc cctttctctt cctctacttc
    14101 gttcacacca gaaagccctt tcgctactgg atatccgatt tccagtccgt taccatctgt
    14161 ttcataaagt tcaaactctg cgccattgat tggcatatta ctgccttcaa ttagtttatt
    14221 gatattcatg tggattggct tgaattcatt tcgcatttcc agtttgacat acaactcatc
    14281 atactctgtg ccttctatgt taggctcttg tacaacagaa agcttgtgat attctccttc
    14341 ataataggaa ttcggatctt taccttccgg tccgccgact ctcgaaccag atgtatattc
    14401 atctacaagc tcgtagtgac ctaacatctc atcttcgttg gctgcatatt ttttgatttg
    14461 gaattgatac gtctcatcta ctaactgata acgtggtggt ccagcgatct ctctgatcca
    14521 aaatatgtcg ctgactgctc cagtttcatc tcctaaaatc ggcatcttcg tttcaaagat
    14581 cgctattccg tccgtggtag ttttacttgt ttcgattggt tcggcttccc cactctctgg
    14641 attttggtca tataatgcaa attcgactcc agccaacggc tcgccatttt gacccttctt
    14701 gctgacttgg aaattacggc ctttatgttc gtaacgaatg gaagaagatg ggaaatacat
    14761 ccgtccagta gtgatatcat tctcataatc ctccaggttc tgaatatagg ccatattgtt
    14821 cactgtgcaa aaatttccgt cttgagctcc cgcatcaagt tttacttggt acttgatcct
    14881 gaccatttcc ccttcaccta aagaaatcgg agaaaattta taagcttggt tttcccgtcc
    14941 gacatacgtt gtcggagtcg ccactacttc tggtggggct tgtccatgac gttcttcatc
    15001 ccatatttct ggctcttggt acttcatgcc gttttctcct ggatctagat ggtaaaactg
    15061 aatatctgcc gatctgccag ctccaaatgg tgagacatta aaaccagttt catcataaag
    15121 tgtggcttgc tggatcgttc tcttgaaggt agaagagata tcctccaagg ctgtaacaat
    15181 gtcattcttg ttgttcgcat tgtaatatcg cggtgttacg ccatcttctt ctaaatcagc
    15241 aatatttttc aatacatttc gtccaatagc atctagtcgt tgtctcccgc taacgtttcc
    15301 atcgattcct aaaccaatgg agaagatact tggcgggttc tcttgatact tgtttttcag
    15361 ccaatgatga taagcaactg tgttgatcgc agatgacttt tcttgcgaag taggactgcc
    15421 attcgtattg ttattagaag aaccatcacc aacccagtga gtcgctttat tatctccttc
    15481 ttttacctct ccataacgat agtgtacatt tacttcatct tctggacggt taaagtcaga
    15541 tgcataggta actggatgag taaaccctgc attagaagta ttcccagaaa actcctcaaa
    15601 atttctgaac aaaacgtcat tatcttcagc aatcttatct tccatcacag tccagttgct
    15661 ccaagctcca ttagtgctcg tacgataact tgactgaatc ggtgcataac tgaaggtcgg
    15721 tgtcccatct cccacaacga ttaggatttt ttctggattt ctattttctc cgccattgtc
    15781 tttatacaat gtttcatagc catttttaag ccctagtgta agcggagttc ctccgattgg
    15841 tgtacgattt aatgctgtat tctcacttcc cgtcaaacga ttgatatcat ttgtcaatgg
    15901 aaagatgtcc gaactgattt gctcatggtt attgatagct gtactattat ggtaaaaatt
    15961 caccataccg attcgaatat ccagactcgg gtcagagagc agatctttac tcatttcgtt
    16021 tactgcctca atcaacgcag catttttagt ttgactattg gtcccagcgg ttaattcact
    16081 catactcgct gacttatcta gaacaaaaac gatatcgatt ggactgattt cctttttctc
    16141 gcccccgatg acatctagat agacattgta aagggttgtt gcatcttcag gatgtagatc
    16201 agatgctgtg ctgcttggca gaacaggttc tacccatttt ttgatgtacg ccccgttata
    16261 atccaagtaa ctgttttcaa aattccgttc atttccccaa agctgaacgg tttgagctgg
    16321 aacgtttcta tcttcttcgc ctgcagttga tccattatta gtagcatagt cgtaatttcg
    16381 ccagttcgct cccccgtttg tataatcact atcatcgatc aagttctctc cgtcatatgg
    16441 cgagcctttg tctccttgat agcgttcatt gctttgtgtc caatttccta acacttttgt
    16501 atagtcattc gttgtaaact tcgggtagcg atgctgttcg ttttcaactt ctccgctagg
    16561 atagatatat tcataggggt cttgcgtagc tgttggccca agacgctcca aaataccgtt
    16621 gaataactgg cgttgaatgc gctggagtgc ttgaccgctg ggtacgattg gttcgtccag
    16681 cttttccatc tcattttctt cgctacgttg aatttcagcg gaatcttctg tatcagtttc
    16741 attttcttgt aattcttggt tatctattct ttcatcaacc accacttgaa gaggtagtta
    16801 cccttcgttc acatcgggga tcttttctgt ttcaaaaatc aatgtgccgg tttctgcttc
    16861 ttttgaataa acttttccga cataccattc ttgaccatcg atcgtctcga cttttaggtc
    16921 ttctccatct tcatcatagg aggatagacc tgttccacga acgtttttta cggtaccaat
    16981 cccggcggct gccgcatcga ttgctaaacg tattctacct tcattggcac tttccttttt
    17041 ttcaaaagcc agatgccatt caagggtttc ttctttttca atatacttcc cttcaatttt
    17101 tgcatagtcc tcatctaaga atacctcatc ctcctttgtt tctgttgact cagcagctac
    17161 atccagggtc gtcatatgat agactgactg gaaatatggt ccaaatagca aactgacagt
    17221 tactaactgc aaaagcttca attttaaccc tttcacatcc ttatactcct ttcaaaaata
    17281 tttgtcttct ttcacaacaa tcaacaaaac aatgcccatc taattataat ttaatttaat
    17341 tttaatttta aatcaaatga tatccttttt agtt
//
LOCUS       DAZKOU010000060        16350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000060 DAZKOU010000000
VERSION     DAZKOU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.061"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..381
                     /locus_tag="ACG827_001937"
     CDS             <1..381
                     /locus_tag="ACG827_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0484069.1"
                     /translation="TSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSD
                     STDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWI
                     TLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(536..1522)
                     /locus_tag="ACG827_001938"
     CDS             complement(536..1522)
                     /locus_tag="ACG827_001938"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0484070.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1545..2111)
                     /locus_tag="ACG827_001939"
     CDS             complement(1545..2111)
                     /locus_tag="ACG827_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0484071.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2125..2541)
                     /locus_tag="ACG827_001940"
     CDS             complement(2125..2541)
                     /locus_tag="ACG827_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0484072.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2998..4932)
                     /locus_tag="ACG827_001941"
     CDS             complement(2998..4932)
                     /locus_tag="ACG827_001941"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0484073.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5234..6169
                     /gene="pplA"
                     /locus_tag="ACG827_001942"
     CDS             5234..6169
                     /gene="pplA"
                     /locus_tag="ACG827_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0484074.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6259..7362
                     /locus_tag="ACG827_001943"
     CDS             6259..7362
                     /locus_tag="ACG827_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0484075.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7375..8328
                     /gene="menA"
                     /locus_tag="ACG827_001944"
     CDS             7375..8328
                     /gene="menA"
                     /locus_tag="ACG827_001944"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0484076.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9142..10488)
                     /gene="gor"
                     /locus_tag="ACG827_001945"
     CDS             complement(9142..10488)
                     /gene="gor"
                     /locus_tag="ACG827_001945"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0484077.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10622..11875)
                     /locus_tag="ACG827_001946"
     CDS             complement(10622..11875)
                     /locus_tag="ACG827_001946"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0484078.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11881..12804)
                     /locus_tag="ACG827_001947"
     CDS             complement(11881..12804)
                     /locus_tag="ACG827_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0484079.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13025..15517)
                     /locus_tag="ACG827_001948"
     CDS             complement(13025..15517)
                     /locus_tag="ACG827_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0484080.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15524..15991)
                     /locus_tag="ACG827_001949"
     CDS             complement(15524..15991)
                     /locus_tag="ACG827_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0484081.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16127..16200)
                     /locus_tag="ACG827_001950"
     tRNA            complement(16127..16200)
                     /locus_tag="ACG827_001950"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16164..16166),aa:Arg,seq:acg)
     gene            complement(16258..16331)
                     /locus_tag="ACG827_001951"
     tRNA            complement(16258..16331)
                     /locus_tag="ACG827_001951"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16295..16297),aa:Pro,seq:tgg)
BASE COUNT         4818 a         3421 c         2905 g         5206 t
ORIGIN      
        1 acctctacca caagtgatag ctcagattca tctaccacta caagtgatag cacggacacg
       61 tcagcttcaa gtgacagtac agatacatcc tctaccacaa gtgatagctc agattcatca
      121 acttcaagcg atagtacaga cacgtcgtct accacaagcg atacacgtca tgcaagtgtc
      181 tccttctcaa aaacaagttc ggataacagg acgaacttta acttaactaa taaaacaagc
      241 aatgacaatg gaaacaaatc aaaaagagca cttcctaaaa ctggaagtca atcgaataat
      301 tggataacac tcgcaggcgt tatattactt gtaatcggtt taagaatcac ttttatgagt
      361 tataagaaat cgaaaaggta aaaaaatatt caagataaaa aagagcaacg tcgctaacgg
      421 acgttgctct ttatttttgc tttttatcat aaatacaaga actacaattc aatttgtcaa
      481 aaaacacgta gatttccttc tgagaaattt atttacgtat cgaacctttc cctattcagt
      541 ctttgcgatc caaaatcagt tgggtaaggg aaaagagatt ctcttttgtt cgaagagagg
      601 tggcaggtaa tttctttatt tccttcaaag ccttttctgt gtaagaggcc gctaacttcc
      661 tagcttcttc cacgcctcct gtatgttcaa cgattttgcg tacggcgtcg acatcttctt
      721 ctgtcattgc cgctttcttc tctaaataag gtagtagctt tggtttgttt ttttgcaaag
      781 agtagatcaa cggtaaagaa tagacccctt gacgcatgtc ttctagtaca ggtttgccga
      841 tttcctggct ctcttttgta taatctaaaa tatcatcgat aatctgaaag gccatcccga
      901 tagaaagtcc gatatctcct gctttttttg cgaagcgctc gcttgttcca ctttcaaaag
      961 cgccaacaga acaacttaat tggaacagtt cagctgtctt acctgaaata ttttccaaat
     1021 attcttttac agaaagttca taattatagc gatcatccat ctgccctaat tcgccattca
     1081 atactttttc catactgcga gaattcaatt ggagactttt caaggaagtg gagtaatcag
     1141 acaatagttt aaaacagcag acaaataaat agtctcctgc atagacagaa acgacattac
     1201 caaactgact acggatatta ggctgaccac gtcgcagatc cgcttcatca acgatatcat
     1261 catggatcag tgtggccgta tggagcattt cgatagaagc tgccaaagcg atggctttgt
     1321 ctcgatcttg ctctggtcca aattgtgaga acaatagctg ataggcagga cgcaacagct
     1381 ttccgccggc atggatcgtt tctttgattg cttgttcgac aggtttgttt ttcaggcgga
     1441 cagtttcttc catccgtttt agtgtttctg tcaattcttt acttaaagca ggatagtttt
     1501 cccacattgg atgtagcttc ataaaaaaac tccttgtgtg acgattattt tttggtaaac
     1561 attgggctcc atttttgttt ggttccctgc tcataatctt tttggaatcg ttgaagtgtc
     1621 tgcacatgtc gtaaaagata attcgctgcg atcccgattg caatccctga taaaatccct
     1681 aaaactgaaa ggactggcaa gtacaacatg actgtccatg attgtgcaat gaaagaagcc
     1741 atcaaaagct gtccgacatt atgcatgaaa ccgccggccg cactaattcc gatgatactg
     1801 acacgttttg gtcctaattg cttgaccaat aacattccaa aataactaag caaagcacca
     1861 ctcatactat aaaggaatgt ggaaatcgtt ccgcctagca aagtagtcag gaatagtcgt
     1921 agccaaagta gtaagaagct gtctcgtttt ttcatggtaa aaatggcaac gatcgtgatt
     1981 agattggcta agcctagttt tgcaccaggc gcaaaagaaa aaggataagg gatcatattt
     2041 tccaataaac caatgatcac tccttgtgca acaagtagcg aaatataaat cattttttgt
     2101 aatttactca tggtgtgtga aggtctaata aatcagactg tcttcgtcac ctccatctgt
     2161 tgaacggact tcgatcacta atttatgtgg caaacagacg atggtttcgc catttttcgt
     2221 tgcccagcct cgacgtacgc agatctggtc atcgcaatct gcttctttta tccgtactcg
     2281 atcgccatct atctcgatca agttataatc tccgtgttca tctttgtaag tatatgtgtt
     2341 gctctcttct ccagccatca aagggaatgt tttgatttct gtgccatcca cacgcagtac
     2401 cgcttctttt tctgctgttg cggtgttttg ctgaagacca aaaatcatga ctggtaaaaa
     2461 agaaagaaca gtcaaaagca aaataataaa aacatcccaa ggtttgagca gacttttttc
     2521 agcaaattct ttaaggctca tttttgttct cctttttcca gattaactca cccaatttta
     2581 gcataaaaac aagaggttgt gacacctgtg tcttgctttg agtaataaga acgaagaata
     2641 gaaaacagct ttccatgttt tctaattctt tgggcttatt accgagaagc agcaggtgga
     2701 acaccgttta taacaagagg ttgtgacaga agtgggcaac ttcaagaaat aagaaggaat
     2761 ttacgaaaat tacttttcaa gtttttgtga aatttcggct tatttttcga agaagctact
     2821 tctggaacac cgtttataac aagaggttgt aacagaggtt gtgacacctg tgtcttgctc
     2881 atggaggctt atctcgggca tcttattcct ttaattttac ataaaaagga ggctgtgacg
     2941 gtttgtctca gcccctttct attgaaatct tgcattcaaa taacgttctt tatctattta
     3001 tttcttgccg tactgggaac ttggtctacc gtaccaccaa ttacctagtg tacgttgtag
     3061 ccatggaatt acccagcgat ccaacccaat cgcacgtccg gaaccgttca tcaatgcgaa
     3121 agcaacaaaa atgaaccaaa tgtttaccca gtaaaacatg cctgataagc agaacgtgat
     3181 caccaataca atcgttgtcg cactgcatag ccatgtgaat aatccagcaa tcaatgctaa
     3241 tgccaaggca atttcaaaga ataccatgaa tttttggaag aacattgcta catcattgtt
     3301 tggcatgatc acttgcatga tctttgtgaa ccagtctggt gacttctcaa tcacagccat
     3361 tggttcttca ccgtatgcat aactcaaacc aaaatgtgca gcttgagtcg ctgcttctcc
     3421 ggcacttgcc cctgctccgc tggcagctgt ggttgcatca ccagcagcag aagcaccact
     3481 ggcaacatct gttacattgt attcccatgt ccaagggaac acactgtttc cagtgaacca
     3541 tgatccttca ccaaaccaag tacttggttt gaacacttca ccattcccaa tgattttttt
     3601 ggaagcttca acgaaccaaa ctaaaccata gaaaatacga agtggcacac tccataagac
     3661 attgccgtaa cgagaagaat gtcctcgagt cacgttgcga tcatctttga tatggaaaaa
     3721 ctcatgcatt aaatattgga acatataata accagaacga atatcaaaga agtatttcaa
     3781 attcacgata tgtttcatta cgatagctaa aaatccgctt aaatggatct tatcaaatag
     3841 gttagctacc ccccatttgg caccaataga aaccataaat ccttgatagt ttcctttaaa
     3901 tttatgcttt tctccgcctt tgatcgaagc cacgatattc gctgcggctg tgtgtcctgt
     3961 ttgttcagca gcttgaacga tctgcggtgt cggtgttcct gggaattctt cataaaagac
     4021 taaatcacca atgatataga tgtttttatc ttcgtagcct tttgcctgca tatattcatt
     4081 ggcgatcaac cgctgtccgc gtgctgattc taatccaaag tcagcagcgt cagaagttcc
     4141 tttgacacca gctgtccaaa tcagtgtgtg agtcggaatg ttgcgtccgt ctttcaattt
     4201 gatatgatca gcagccactt ctacgattgg tgcattcaat aataattcca cgttgtgctt
     4261 acgcaaatag cgttcagctt ttgcagcatc atttcgatcc aacatattca agatcgtcgg
     4321 cattgcctct acgaccatca gtgtgatttc gtttggatct aatttataat ctttggctag
     4381 gcgatttttc cagtcgatca gttccccaat catttcaata ccggtaaatc cagaaccaca
     4441 gacaacgaaa gtcagcatgg ctttacgcag ttcatcatct ggttccaatg cagcttttgc
     4501 tactgtcttc tcaatatgtt cccgaatcct tacagaatct tcaaatgacc aaagggtgaa
     4561 tccgtgtgct ttgacaccag gtgtaccaaa gtcattcggc tctccgccca ttccaaggat
     4621 cagctgatca aaaggatagg aacctaattt ggtcttgacg atttttcgtt ctttatcgat
     4681 accgattaca gtgtctgtga caagttgtac attcttttga cgagcaaaca aatgctgcag
     4741 atcgtattgg attgcttctg gttctacacg tccgccagct acttcatgca actcggtcat
     4801 catagtatgg tacgaatgac gatcgatcag agtaattgct acatcttctt ttttcagttt
     4861 ctttgccaag tattttgtag ctgaaacacc ggcatatcct gctcctacaa cgacaatatt
     4921 ttgctttgcc attgatcatc cgctccttat agactatcta tataatgaat atatgataat
     4981 tcttttagtt tgatacttat tacacaatct ccatatatta tatagatgtt ctgttctttt
     5041 gtctaactcc ttttggattt ttttcaaatt ttaaaataaa gcgttttttc acgaaatttg
     5101 ctgaaagaaa acgtttgaaa tattacatgg gttatttatc aaaaatagtt gacttatagg
     5161 ctaaaaagat tagaatgtat aatgaatcac aaagaaaaat tgtggactag aaatggaatg
     5221 gtggggtatt aaaatgaaga aaaaacgttt tgtaactgga tttgccgtat tagcattttc
     5281 agctctagtg ttaggcgctt gcggcgcaga tgacaacgga agctcaaact cttcttctga
     5341 atcatctaca gcacaatctt cgactgcaaa aagcacaaca gaatcatctg caaaagttgt
     5401 agctggcgga gatttgcaag acggcacata taaattagaa gaaaaaaatt attcaaatgg
     5461 ttaccgtgca gtctttgaaa tggttgtaaa agacggcaaa atcactgaat caaaatatga
     5521 taacgtcaat gaaaacggcg aatcaaaaac aaaagatgca gaatacaaca aaaacatgga
     5581 agcaaaatct ggcacaaacc cagaaaaata cattcctgag ttgaatgacc aattcttaaa
     5641 agcacaaagc gcaagcggtg tggaagtcgt aactggagct actcactcat ctgaaagctt
     5701 ccaaaactac gcacaacaat tgatccaagc tgctcaagct ggtaacacag atacaatcga
     5761 aatcgacaat ggtgctgatt tgaaagacgg cacttacaag cttgaagaaa agaactactc
     5821 taacggctac cgtgtgcaat tcgaaatgac agttgctggc ggcaaagtaa ctgaatcaaa
     5881 ctttgattat atcgacaaag acggtaaatc aaaacaagat gatacagaat ataatgaaaa
     5941 tatgaaagca aaaagcggta cagaaccaaa aacttacatc cctactctga acgacgaatt
     6001 cgtcaaagca atgggcgaag aagatggctc acctgctaat gtagaagttg ttacaggtgc
     6061 tacacacagc tcacattcat tcatcatgta cgcacaacaa ttagtgaatg cagctgaaaa
     6121 aggcgacact caaacaatcg aagtagataa tatcgtaaca gaaaaataat cacataatca
     6181 tactaataaa agttggaggg tgggacgata gccagattag gcagtcgtct cacctttcaa
     6241 aatgaagaga ggaaaaagat gcggaaaaaa ctactatttt ccttaatcac actgggcatc
     6301 tcttttatgg tgctaactgg ctgcagcaac tcagcaaata aaacaaccac tgaaagttca
     6361 gcagctacca agatattgaa agatccatat tctgatgaac agttcttgtt agggacttac
     6421 gttcgtatcc gtgtgtatga tgaaggcaaa gaatcagcat tgaaacctgc cttcgatcga
     6481 gtaaaagaac ttggtgataa aatcacgatc aatcaaaaag gttctgagat cgatgaagtg
     6541 aatgagcaag ctgggatcaa accagtaaaa gtttctgatg acgtctacac gttagtcaag
     6601 cgggcctacg aatatagcca agattctcaa ggcggattcg atatggcgat tggtgcaatc
     6661 acacaattat ggcgaatcgg ttttgatgac gccagaaaac cttcccaaga agaaatcgat
     6721 caggcattga aattagtgga ttatcataag atcgaactaa atgacaaaga aaaaactgtt
     6781 tatctaaaag aaaaaggaat gatcatcgat ttaggtgcaa tcgccaaagg ttacattacg
     6841 gacgaagtag tgaaagtctt gaaaaaacaa ggagtgacca ctgccatcgt tgacttgggc
     6901 ggtaatgtct atgtcttagg ccacagccca agaggcgaaa accaagactg gacagtcggc
     6961 atccaagatc cgaacatggc tcgcggatct gttttaggtt ctattaaaga acggaacaaa
     7021 acattagtta cctctggaat ttacgagcgt tatttagaag tagatggcaa aaaataccat
     7081 catttgtttg atccaaaaac gggttatccg ttcgacaatg atatcgcaag tgtcacgatc
     7141 ataacagata aatcaattga tggtgacggg ttgtctacag cagtcttttc aatgggtgtg
     7201 aaaaaaggac tggaatacgt cgaaagcgaa ttgaataacg ggacggaagc gatcttcgtg
     7261 acaaaagacg ataaagtata tgtgaccgat ccaatcaaag acaccttcaa attatcggag
     7321 gactcccatt acacgatggg tgaccgttct gaactaaaat aaggagtacg aaccatgaca
     7381 ctaaaaatct ttttagaagt agttgaaata cgaacaaagg tggctagtgt atttcctttt
     7441 gcggtagggg tattattctc tatcgctttt tttcatgaga tacattggct aaacactttt
     7501 ttatttttta ttggaatgct agtttttgat ttagcgacta ccgctatcaa taattacatg
     7561 gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat tggccgcaga
     7621 aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt tgtttttgtt
     7681 atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg atttatctgt
     7741 tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat gcctttaggg
     7801 gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt ttatgtcaat
     7861 acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca atttgctttg
     7921 gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt ggtgtttacg
     7981 attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat tgaaaatcat
     8041 cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt ccagctattg
     8101 atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta tgaatggccg
     8161 atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca attcaagcaa
     8221 acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt attcaatgcc
     8281 agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca gcatgaagag
     8341 gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa tagctcctta
     8401 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttatcag
     8461 gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga aaatggcttt
     8521 ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttatc
     8581 agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc
     8641 ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt
     8701 cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg
     8761 attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt
     8821 aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg
     8881 ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg
     8941 cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt
     9001 ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa
     9061 aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct
     9121 gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg
     9181 gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa
     9241 accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc
     9301 ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta
     9361 cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc
     9421 ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac
     9481 aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct
     9541 accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa
     9601 aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa
     9661 ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa
     9721 aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt
     9781 gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta
     9841 catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg
     9901 atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat
     9961 gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt
    10021 tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc
    10081 gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat
    10141 aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc
    10201 accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt
    10261 ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt
    10321 acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa
    10381 ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc
    10441 aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct
    10501 tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt
    10561 aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc
    10621 cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta
    10681 aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt
    10741 caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag
    10801 aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt
    10861 caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag
    10921 ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt
    10981 cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg
    11041 ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct
    11101 tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca
    11161 ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt
    11221 gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa catccgccac
    11281 ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac
    11341 acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca
    11401 cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga
    11461 attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg
    11521 gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac
    11581 cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga
    11641 ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag
    11701 ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa
    11761 tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg
    11821 gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc
    11881 ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg
    11941 atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc
    12001 taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg
    12061 agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc
    12121 attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat
    12181 agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt
    12241 ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg
    12301 gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc
    12361 cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc
    12421 ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat
    12481 acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac
    12541 atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg
    12601 atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac
    12661 caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc
    12721 accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc
    12781 tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata
    12841 gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac
    12901 aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa
    12961 atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa
    13021 gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac
    13081 cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct
    13141 cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt
    13201 caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa
    13261 tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga
    13321 tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag
    13381 gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag
    13441 taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat
    13501 ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta
    13561 aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca
    13621 cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac
    13681 cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt
    13741 ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg
    13801 aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac
    13861 caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg
    13921 ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca
    13981 agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa
    14041 ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa
    14101 ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag
    14161 cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt
    14221 ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg
    14281 ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa
    14341 cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt
    14401 catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta
    14461 cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt
    14521 catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga
    14581 tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat
    14641 caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat
    14701 cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca
    14761 ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt
    14821 cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc
    14881 gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca
    14941 gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc
    15001 cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc
    15061 cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc
    15121 tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca
    15181 tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta
    15241 aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac
    15301 ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat
    15361 ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa
    15421 catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta
    15481 atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat
    15541 aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat
    15601 tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa
    15661 ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat
    15721 catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg
    15781 caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg
    15841 taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca
    15901 tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt
    15961 caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac
    16021 cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc
    16081 tttagaatca acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga
    16141 gtcgaacccc taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc
    16201 atattcaatt taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg
    16261 ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga
    16321 gctacttccc gtttaaaaaa taaaaaagac
//
LOCUS       DAZKOU010000061        16224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000061 DAZKOU010000000
VERSION     DAZKOU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.062"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1583)
                     /locus_tag="ACG827_001952"
     CDS             complement(<1..1583)
                     /locus_tag="ACG827_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0484082.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVT"
     gene            complement(1570..3258)
                     /locus_tag="ACG827_001953"
     CDS             complement(1570..3258)
                     /locus_tag="ACG827_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484083.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            3404..5041
                     /locus_tag="ACG827_001954"
     CDS             3404..5041
                     /locus_tag="ACG827_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484084.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(5186..5926)
                     /gene="truA"
                     /locus_tag="ACG827_001955"
     CDS             complement(5186..5926)
                     /gene="truA"
                     /locus_tag="ACG827_001955"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0484085.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6117..6752)
                     /locus_tag="ACG827_001956"
     CDS             complement(6117..6752)
                     /locus_tag="ACG827_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0484086.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(6899..8119)
                     /gene="sstT"
                     /locus_tag="ACG827_001957"
     CDS             complement(6899..8119)
                     /gene="sstT"
                     /locus_tag="ACG827_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0484087.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            8338..9336
                     /locus_tag="ACG827_001958"
     CDS             8338..9336
                     /locus_tag="ACG827_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484088.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            9532..11367
                     /locus_tag="ACG827_001959"
     CDS             9532..11367
                     /locus_tag="ACG827_001959"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0484089.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(11427..12143)
                     /locus_tag="ACG827_001960"
     CDS             complement(11427..12143)
                     /locus_tag="ACG827_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0484090.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(12370..12912)
                     /locus_tag="ACG827_001961"
     CDS             complement(12370..12912)
                     /locus_tag="ACG827_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0484091.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(12917..13144)
                     /locus_tag="ACG827_001962"
     CDS             complement(12917..13144)
                     /locus_tag="ACG827_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0484092.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(13223..15103)
                     /locus_tag="ACG827_001963"
     CDS             complement(13223..15103)
                     /locus_tag="ACG827_001963"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0484093.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(15292..15906)
                     /locus_tag="ACG827_001964"
     CDS             complement(15292..15906)
                     /locus_tag="ACG827_001964"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0484094.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            16048..>16224
                     /locus_tag="ACG827_001965"
     CDS             16048..>16224
                     /locus_tag="ACG827_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0484095.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         5170 a         3066 c         2531 g         5457 t
ORIGIN      
        1 gtaacaagat aagaaataat agtcgttact gtataacttg tgcttacttt aatatcagca
       61 actcctttga ctccgcttat ttgtaccaat ccattagcat tcacctgctc ctttacaggt
      121 tgatgattta tttctacatt cgttcctaaa taagcatata caggaagtgt gattacttgg
      181 ctcttctcat tagttactct atatatgata gaactaccag ttgttttata aggaaggttt
      241 acccattgac tgccaatctt tgcttcgtgt tttattatat gctccttatt ctccaatgaa
      301 gcgataggag aataatcata taaaaaataa tccattcctt tagtggtata cgcctctact
      361 gtttctttgt ttacaacaat atgtcccggc tgagaaatta ttttttgatt gaaatttttt
      421 ttactagaaa cagttaaagt gattaataac aaaacagtca taaataaata aaatagttct
      481 cttgtcttat tgtatcttct atttttgaaa aatatcatcg ttaatatcag agaagagaat
      541 attatttgca agcttaagat ccgccaagga aattgtaaca atttggctgg cgtattttgc
      601 agaagatacc atggcaccaa actagttgta gctagccaag tcaaacacgt accataataa
      661 atcttttttt ctatgtttgt taatttttta aataaaatca ggggaataca taaagataac
      721 agaccaacta gtcccattga gtaagtagtt aagtcgttgt tcatagcttc agtcagacta
      781 tcgaacacat tagatgcatg agcatataaa tttgtcacgc taggtttgtt gattttttgg
      841 tagagcattt gttcaaacat cggataccaa aaagcactcg tcagaaccaa tgtcattcct
      901 gccgcactaa ataatttttt aaatatagca ataaaatctg cattcttgaa acctttagaa
      961 accaaacgaa taaaaataaa aatgataatt aatgctgtac tcattaatgt tgatagtaca
     1021 tggctgtaca ctagcaaact catactcaat gctagcttta cagacggata cttttcttct
     1081 tttccagaga tgatttgata agcataaaat aatacaggga taacaagtgt tattgcgata
     1141 gcttcggcaa ttgcggaacg atagtagatg tctactgtcc gataagcaga gaaaatatag
     1201 agacaactaa ataaaaatgc cgcttttttt tgttttaaaa accgctcgcc ataatgataa
     1261 gctacgagtg ttgtacacaa gttcaataac caaacgtaaa taatatagct tacaatcaaa
     1321 ttttttgtca tccaatataa cattacagct ggaaaaaaag tcagaaaggg ataaaaataa
     1381 tttacaccat atccagtata attgaatgtc tcataattaa tcggactggt aaaaatagtc
     1441 gataacccct tgattcgaga taagtgaaaa gaaagatctc cagtggtata aatactctgt
     1501 gtatttttga aaaagatgaa agaatatata ctactaacta taattaatcc actcaatatt
     1561 actacttttt tatttatttt cattcttctt gtttatctcc tttttagtca catatcgaga
     1621 atctttcaac ttattaggtg caaatcgttt aaaacttgaa tacaccacta aacaataagt
     1681 cagaatactg atccataaag ccatgagagc agttggagta tattttgttg taatttctat
     1741 cttattttct ccagcattta catccacatt cactaagcca tgttttttat aagagtcatt
     1801 tacgacttta ccgtttactt ttacctgtgt acctaaataa gcatatactg gtactgtcac
     1861 tttttgcttt ttgttgcttg ccactgtgta agtaatgtta ttatctgcag ctttatactt
     1921 ccctttttcc cacgattctc ctacttttac ttcatgtttt tctaagtgag gcctttcttt
     1981 tatcgcttct gttggggcgt aatcgtataa tcctcccata tctgatgttg tataacgtgg
     2041 gagtgtttct tcattgataa caatatgacc gtgttttgat tggataactt ttgcgtaatt
     2101 ttcttttgct gctatagacg tgacgagtag aaacaccaca gtagcgccaa tgattaacca
     2161 tgaatatttt ttattcatag tctttttcat aaaaacaagc gttatgatca ttgaagaaaa
     2221 aaagatttgg atacctaaga tacgccaagg aaactggagt aagcttatcg gtgttttttg
     2281 aaacaaaaac catggaaata aattagttac gcatacccat gtagaaacta cgccaacata
     2341 gatgattttt tcttttttct ccatttcttt cagtatgaat aaaggaaaaa tcaacgcaac
     2401 caatcctaat aaccccatag tgtatgttgt aatatcgtta tttaacgctt gaatgatact
     2461 atctgaaata gaaagtgctt ctagctgcaa atctgttttg aagggactgt gtatttcttg
     2521 ataattcatc tgctgcaaca taggatacca aaaatagcta gttaatgcac aagtcaaaaa
     2581 aacagccttt actaaattca cgaccacaaa ttttatatcg ttttttattt gcttgcttaa
     2641 aaacaaacga actataatca tacaagcaat caatgcacta cacaatagtg tagttaagac
     2701 atgagtatac acaagtagac ttatactgac agataaagcg atgacttttt tttctctatt
     2761 gaatatcaat ccataagcat aatataaaac agctgggata atcgtaagag cgattgcttc
     2821 tgcaattgcg gatcgataat agatatcaac tgttcgataa gttgcaaaag tatataaaca
     2881 actaaataaa aaagcagcat ctatcttttt aaaaaacttt tctccataat aaaaactaat
     2941 cagaatcgta caaatattta ataaccaaat atatagaaca taacttaata ttaagttatt
     3001 tgaaattcca taaaaaacga ctgcaggaat tattgtaaga aaaggataaa aatagttcag
     3061 tccatctcca taattgttaa atgttgtata gttgattggc ccttcaaaaa tagaagataa
     3121 ccctttgatt cgtgaaagat gaaaagataa atcacctgtt gtataaattt cttgagcatt
     3181 tctcataaaa tttactgaat ataaagtact aagtaagata agaactacta aaagaaatgc
     3241 ttttttactt attttcatag atttgttgtt ctcctttttt aattctattt tcagtatact
     3301 agaattgtct ttttttaata agttttgcgt taaatttttc atagcactag tatgatatac
     3361 tagaaaagaa tcaattttta ttacttttaa ataggagaaa aaaatgaaaa aaaacaaaaa
     3421 ccaaataata gatatcattt ttatgttatt tctaagtttt gcttacgtca tccctctatt
     3481 catgtctaag ggaatctatc atagtgtcaa tcaggatact tactttcatt tatctcgaat
     3541 cataggacta gataatgtat ggagttctcc agtaaacttc aataacttcg atcatcatgg
     3601 aacaatgatg aatatttttt atccatggct tactctttat ccagcttttt tattttataa
     3661 aatgatggga aatcttgttt taagttataa tatctactat ttttttatca cttttttaac
     3721 aatggtcgtt tcttattttt ctatgaaaca aataaaaaac aatcgttata tttcgctgtt
     3781 attttcgatt atttatacat tttccgccta tcgtgccatt gacatattcc atcgtgcttc
     3841 attgggagaa gctgtcgctt taacattttt gcctttgata ctaatggggt gttacgaaat
     3901 ttatattaga gattaccaaa aatggtactg gttgtctatt ggcatgacgc tagtagtgta
     3961 tactcattta ctttctgtcg ctatggtttc tgtatttatt ggaggaacgt tgtttctttc
     4021 tttttatttt tgggaccaaa aaatcgctag actcttaagt cttttaaaag ctaccgtcct
     4081 cacctttttt ttaagtgcgg gattcttaat tccttttatt caacaatcta gagcacaaga
     4141 attgaaagtc ccactgggaa aagaattaag tggtatggct cctagtgata tgctgacaca
     4201 tattttaaat aataactata acaactatac tattggatta ttcttatttc tcggattgat
     4261 tggtgctttt atttttataa aaaaattaac tgctgatgat ttatttattt tctttcttgg
     4321 tgtatttgtt cttttttgtt caaccaattt atttccttgg caacttttta accatacacc
     4381 agttaagagt ctacaatttg tctggcggtt aaatggtttt tcttctttat ttatagccta
     4441 tacgatgagt atagtgatct attatatttt ttctactaaa aataattcat ggaaaataat
     4501 ggtctttacg tttcttgcac taatattgca tctctcagga gtaagcaatt ctatccatgc
     4561 taacaaagat tcattaacac ttatagcacc tgaagacgct gttgctatag ctgaaaatta
     4621 caatcatact gattatgcaa acaaagagtc aatctatcat cctgatatga ttaataatga
     4681 tcaatattta ctaggtaacc aggaaataaa tccaacaact cattttatga gtaacaaact
     4741 tacaattgaa ttagataatt cgaataataa aaatactgtc ttgacgacac ctatttatcg
     4801 ttataaaggg caagtggctt ctattaatgg caaactagta caaacaaaat tatctaagtt
     4861 tggcacaact gaattaacta ttcctccagg tataaataaa gtcgttatta cttatcagta
     4921 cacaaaacta gcaattgctt caagatattt atctatcgtt actttgattt tatttttact
     4981 ttatcgattt actttttcta aacaaaaaca accgagaaga gaagtacaac attctcacta
     5041 gatttttatt taagaaagac tttttagctg tacacataaa caggctaaaa agtctttttt
     5101 tatatttctt taatttgctt catacattat ttaacatcga tatttatact gtgaaacatg
     5161 cgtttcatcc attatcaaag tatttttatc gataataaac ttcatccaaa aacagacctt
     5221 gtgcagggac ggtttctcct gcatgttgcc gcactttgct ttcaaatatt tcatcgattg
     5281 tttctatcgg catggcacct gtgccgattt ctagtaatgt ccccatgata atccgcacca
     5341 ttttatataa gaatccgtct ccgacaaaag taaaatgaag catttctccc tttcgttcga
     5401 tagataattc atcaatcgta cgcactgtgg atttttttga ttttttcaat gcagaaaaac
     5461 caatgaaatc atgtgtaccc actaattttt cgcaagctgc attcagcttg tccatatcca
     5521 actcttgagg gaaataaaag ctgtggtttc gctcaaatgc tgaaggaatc acgttgttcc
     5581 aaacataata gctgtatttt ttgcccaccg cattatatct tgaatggaag cgctcaggca
     5641 tttcttcgat tcttttgatg acaatatccc gcggaaggta acgattcata aagtcgatca
     5701 tttctgccag tccaatttct gtgtttgttt taaaatttgc tacttgtcct cgcgcatggg
     5761 tgccagcatc tgttcttcct gatccaataa tttctatctc ttcatttgtc atctgatgca
     5821 atactttttc gatttttcct tgaatggttt tatcgtcatc tccaagtctt tgccatcctg
     5881 aatatctttt tccgtcatat tcgattgtca acttgatgtt tctcatattt tagtggctcc
     5941 tctgtcttcc atttggtctt ctctctacct ttttaagatt gtagcacaaa atatagctcc
     6001 ttgcttctct aagacagaag ctatacaaga aaactgtgga aatatatatt tcagaaaaac
     6061 aaaaaacagc caccggatga agtatccgct gactgttcaa tttgacggct caagttttac
     6121 tgtttgagat gcaagatact caaccaggca atgggcacat aattaaagaa aaaactgatt
     6181 ctttggcaaa aacctatttt attcagtaat ggccaagaat ttatagaagg aattcttgcc
     6241 agtccataaa tcgccgcaaa aagcaaactg atcacagaca gtccaaggta taagtggctt
     6301 acgtccattt gcccattttt tcgatagatc aagaaaagaa gaactggaaa caataaaaaa
     6361 ccactgtaac ctaaacctga tccgatatta tggacccatg tcgagacatt ccacgaagat
     6421 tcatgggtat tgatactgaa caaacctgta aagatacaat cacctatacc atagaacgcg
     6481 atggctgctg taactagggt agctaacaca cgagatgtac taacaaatgc agcatacaat
     6541 gcaggcaatg acaggatgaa aaagatccct gaaaccacag accaaataag aaacgctttt
     6601 cttacaggac tggaaacctc tccaaataca ctgatcacgc tcttcatttg atctaattca
     6661 ggataataca atcctaatac gtacggagtc aaaaaatcac tcagcacgcc taataataaa
     6721 aagaaaaaac caaatttacg taaaaaatcc atgatacgcc tccaataaca attacctatg
     6781 cttttaattg ttattataaa cgactttatc ttgatttaac agaaacctct atttacacga
     6841 acatctatat aactcattaa aaactcgaat cattttaaaa atactaaata tctaacgttc
     6901 aacgttcact taatccatct aaacaatctt tcaatttgat tttttcccca cttcttttac
     6961 gcgcgcctag ctctcctatt gcagtaaaca acagatcact tgacgaattt aaggcagttt
     7021 ccattgaatc ctggataaca ccgataataa atccaatacc gacgatttgc atcgcgacat
     7081 cattagagat accaaacaaa ctacatgcta atggaatcaa taacagtgaa ccgcccgcaa
     7141 cacctgaagc tccacaagca gctaaagcag acaaaataca taaaaggact ttcaaaacaa
     7201 aaggtgcttc aatccctaaa gaatgtacag cagtcaacgt catcaaggaa atggtaatcg
     7261 cggcaccgcc catattgatc gtagcaccta acgggatcga tatggaataa gactcttttg
     7321 tcaatttcaa tctttcagcc aacatcatgt ttatcgggat atttgcggca gaacttcggg
     7381 taaagaaagc gggaagtgcg ctttctttca aacaaaagaa aatcaatgga tagggatttt
     7441 ctttaataaa caaaaacgtg aggaatgggt acacaactaa tgcgacaaac agcatcgtac
     7501 cgatcaaaac aagtaaaagc tgcagatact ctgccaatcc agcgattcca gtcgtagcaa
     7561 ttgattgata aaccaatcct aaaatcccta tcggagcaaa tgcaataatc acttgtacga
     7621 cttgtgacaa cgctatagat atctgatcca ccaactcttt tgtcgattct gaacgtgctc
     7681 gaaaagctag tcccaacaat acagcccaga ataagacgcc aaggtaattc gcatcaataa
     7741 gagattgaac aggattttgt gtgacattcg tcaacatagt agataaaaca ctttccaaac
     7801 tttgcggtgc tttttcagat actgtttctt gtaaagtaat aggaacagta aaaattgaac
     7861 ttacaataac tgctacaagt gcagaaagca atgttcccac taaatataag atcaaaatag
     7921 gcttcacgaa tgtcttattc ccaattttat gcttggcaat tgacgccatc gtcaagaaaa
     7981 aaaccagaat aggtgcgatt gccttcaagc aactgacaaa caacgttccc aatattgaaa
     8041 taaatgtcca tgtggggaaa aagataccaa acaatattcc gactaaaata ccaacaacaa
     8101 tacgttgaac taacgacata tccaaaaact tctctaacaa atgaccaccc tttcacaatt
     8161 ttaacaatca ttgtatcatt ttatgcaaag aaactaaagg tataattttc taaaaattct
     8221 aacaatacaa acaaccattt cttgctaatt ttgtactttt tctgtctaca cgttgttttt
     8281 tgagatattc taagttagga tgttattaat gggcaactta tcaggaaacg ttttcttatg
     8341 tttttgtcca atttttttgt gaggtgttta ttcttgaaaa aaataaagaa ggagagttat
     8401 ggcttcctga caatctttgt cgcttgtata atgatggttt tacagcgtat attgatttca
     8461 gcagtcacta ttcctacata tgcaacaaac cctggtcgat tctacatcta tacattttta
     8521 caaacttccg ttgttattgg tgcaaatttg atcccacttt acatgggtta ccaatatcaa
     8581 aaaatcgccc gtttaaaagt tttacattat ttaagtcgtt ttgcctttat attcctaata
     8641 gccacactag tttgcaatat ctttttttac gtacaagctg gcagcttgaa tattcgtgat
     8701 tattggctta tcctaacacc tatcagtcag aattacttta cttatgctgt ttcttgtact
     8761 ttgctttttt gtagtattcc ttatacagtt ggatttttaa ataaattatc gaaaacaaca
     8821 attaaatatc tactattatt tttaacaatt ggattagttg gctgctctac tctatttaat
     8881 aaagacccgt ttgcattaca gaatggacag agtattttat ggatttttta tttattttta
     8941 atgggttatg gtttgaaaga atggaactgg aaagaaaagg tccgctataa gcttcctcaa
     9001 gtatttatca gcttgattgt tttattcggt ttgattattt tgatgacaca aatttcactc
     9061 atcatcagag gaaatacaga tacagcgctg aggttttcta aaccatattc tctcttcgca
     9121 atgtattata ctttctcttc gtttttattt ttagaaatgc tatctgataa attaggtata
     9181 aaaggaagag gaaattttct tgctgtattt ttgattgtca ctcaagttat atgtaatttc
     9241 cctttagttt cttatcgcgt atctacattt atgaaacgtg aatttccaaa ctcagggaag
     9301 gcatggctga taaatatcgg gcagtttttt atttgatatt tattagcagt gtccctccta
     9361 gttatctttt tattttatat acaaaaaatc cgtttcattc atcgtttcat cgaatacttc
     9421 gcttttgatt cgattgaaca attaactcag cgttggaaaa aaagaattca ttggcttttt
     9481 gttcataaaa gttatttttt agttgcagca tttttttact gctttacctt cgtgcagatt
     9541 ttcgttttag aaccaaaaga aggttggaaa gaaactatcc aggttgcctt aaaaatattt
     9601 actcagcgac aagcacccat gatcctgaca atcatcatta ttatgggatt tttcttctta
     9661 ctattgttga tttccaatcg tttttggtac gccttgactg ctaccttgat cattaactta
     9721 ctgctcacta tttcgacagt aatcaaaatg gaaatgcggg aagagcctgt ctttccttca
     9781 gatttaaaaa tgctgaccgg attgtcagaa ctattatcga tggttagccc agtcttatta
     9841 atcgtgggcg gattgatcct tctcctatta ttgattacta gtattattgt acaacgtaga
     9901 ctacaaaaac agtattcctt gaagattcat tggaaacgtc gatttatcag tatcgctgta
     9961 ttattaggat gtttttcggg tgtctttttc attaatcaca aaaattcgcc ttctttcttg
    10021 ctgtttaatt tatttaaagt aaacaagact ttctttaacc agcaaggcgc cgtaaaagat
    10081 aacggaccag tcattcaatt cctaaacaat atcgacatta aaatcatgga agaacctgct
    10141 ggctattcga aagaaaaaat taatggaata atgaaaaagt acaacacaaa ggcagatgaa
    10201 atcaaccaaa caagaagtga ctggttaaac aatcagacga tcgttctcaa tttaagtgaa
    10261 agcttcagtg atcctgcacg tgtccctaat ctgacagtac ctacagaccc tattccttac
    10321 atcaaaagca ttataacaga aggaacagga ggattgatgt tatccgtcgg ttatggtggc
    10381 ggaacagcta acatggaatg ggaaggactg acaggcttga gtatcagtaa cctgtctgct
    10441 tcgttagtta caccttatac ccagctagtg gagcgtcaga aaataagtcc aaatattacc
    10501 gatctattcg atgaaaaaat agccatccat ccttttacag ctgctcttta tcgcagaaaa
    10561 caagtgtttg ataaatttgg ttttgacagg ttctattatt tagacagtcc ggataaatta
    10621 acttatacgg ataaaataga acaaagccca cgcatatcgg atgattcagc atacaaagaa
    10681 accttgaaag ctttacaaag caatacggaa acgtcacaat tcatccaatt atccactatg
    10741 caaaaccata tgccttatga tgattactat agtgaattga actacactgc tgaaggaact
    10801 gctgtcattc ctaatagaag aaaagaatta caaacttata tgcaaggcct tcactatacc
    10861 gatacagcag taaaaaaatt catccaagaa atcgacaaaa tcgataaacc gattaccttt
    10921 gttttttatg gtgaccattt acccgcaatc tatagtggga atgattcgaa aaaatatgga
    10981 ttagaacagc accagacaga ttatttcatt tataacaaca agtatagtcg tgaacaagca
    11041 gctacgttaa ataaagaagt tgttgctcct aataactttg gtgctatggc tcttgagcat
    11101 gcgaatatca aagtaactcc ttactatgcg ttgttgacag aagtatctaa tcatctacct
    11161 gcattaacaa ttgatcctac agaaagcttg tctaatcgtt ttaatggaaa acaggtcttt
    11221 gttacagagg atagtcaaat tatccaagaa aaaaatttaa ctgagaaaca aaaagaaatt
    11281 ctagaagatt atcgtctgat acaatatgat cttgttgcag gtaaccagta cagtgcaaca
    11341 tgggcgacac aaaaaataaa agactaacaa ataaactgag caaggggttg tgataaaaga
    11401 catgtcacaa ccccttgttt atctcattaa tttgcttgtc cataaacgat ttgattaaag
    11461 ccatttttca attcattatt cgctgtctcg ttgaagtaaa gataattatc gggattcccc
    11521 tgattttgtt gtgtcaccaa tacttttggc tgattttgct gaaacccgaa tagatagacg
    11581 tgctttttca gatatggttc tgtctctgca tcggaataga cggctgtgat ttgatagtct
    11641 gcttgacctg tcccactttc tgaccattct gcagaaacag gcactccacc aatagccatc
    11701 ttccactccc cgttcaaaat agcttgaggg actttaagac catataagtc cacactcatt
    11761 tgattcgtat aacttttgta ttgttgtccc attgtttggc cccatttggt cataaatgcc
    11821 tctaattgtg cggatttgtc tgcattccac agtgtttgtt caacttgttc gctgctttct
    11881 gaagaggaag atgaagcctc tacagaagaa cttgaagtag atgtttgagt tgtttcagtc
    11941 gttttggaag actctgacga agtagaggta tctgctgttt ctgtacttgc tgcagttgac
    12001 ttgctctctg tgctagttgt tgtagtagtt gcaactgttg ctttcgctgt ttcttttgtt
    12061 gattcttttt tcgattcatt cccgttggta caaccagcta acaaaaaagc tagaccaatt
    12121 gttactaaaa tccttttttt caattaaaaa actcctttcg attactccca cttctttatt
    12181 ctatcaaata agcaatgtga ttttattgat ttttcgtatt ttctcatcaa tctttcaaat
    12241 cttctaaaaa ctgagaagca cccgctgatg ttgtctgtaa tacatcagcg ggtgcttctt
    12301 ttattttaat cttcgtgatt ttccaattta cctcacgctt tatatttgta tttgaagaaa
    12361 catagacttt tagtcatcat cgtcttcctc atccaaatct tgtcggacag tttttccttg
    12421 gagtctttgt acttggtaga tgatttcttt attgaaactt aaaagttcaa caagcaattg
    12481 ttgcaagcca tatttttctt ctttttcagc taatttgatt gcccgtgtga taaagacatt
    12541 ttggaaatca aaatcttttg caaagttttc caacattgtt gaggctggta aatatttgtt
    12601 ttgaccatgt tcgtaaattt ttccataacg catgacttct tctaaagtag acggaacgat
    12661 ttcattttca atcactaact catgagagat tttattgaac catctcgctt cttgatcagc
    12721 atattctgaa aaaagggtac ttaattcttg cgaagatgta cccaaaacat acaattgtgc
    12781 ttggatcaat ttcatttctt ctaatttaaa atttgctaat acatgcccca ccatggcgcc
    12841 agcagttggt ttatgatggt cgatatctgc ttgttttgtt tcttgaacaa atttttcttc
    12901 cactatattc attgatttac gcctctttta cttttgctga ttggacttca taacctaatt
    12961 tttccacaac tgcagctaat tgttcagggt ggacttcatc tggatcaaag tttgctttga
    13021 ttttgctcgc attaaacaaa actttcgtat tgcttacccc aggctgttga gaaaccgcat
    13081 gttcgatttt ttgcatacag cttggacaag ctagtgtatc taattgcata ataatttttt
    13141 tcatcgtagt tcttccttcc tttttcttga tagtcttatt ctagattaca gcttccataa
    13201 aaaaattgat tcttatcaag ttttacgatt tttcttcttt gctttcgtgt ccaaaataca
    13261 ataaacgcat cgcgttaaag ataacgatca agatactagc ttcgtgaacg aacatcccac
    13321 tagccatgta aataaacccg gcaaataagc cgttgagtaa aaataagacg acaccaatgg
    13381 caatcactac attttcacgg gtatttaaaa cagttttctt agccaagcgg taggcatgaa
    13441 ccaacgattc aaaattagat tgcatcaaga caacgtcact cgtttcgata gcaacatccg
    13501 ttcctgatcc catagcaatc ccgatatcag ccgcagccaa agagggagaa tcattgattc
    13561 catctccaat aaaagcaacg cgcaatcctt gttcttggta ttttttcaca aattgaacct
    13621 tttcgtctgg taataattca gcatggactt catcaatgcc taacatatca gctacataat
    13681 cagcagtcat ctgattatca cctgtcagca tcactaactg ttttgctcct gcttgtttca
    13741 acttagctaa ttgttcagcg acttctggtc gaatcacatc tgacacacct atgatttgag
    13801 tgattttttt gtcgattgct acaataatga ccgagctgcc tgtttgttgt aattgatgta
    13861 aatcttgtac ttgctttggt gtgagagaaa gtttgtgagc ggcaatcaat ttggtgttgc
    13921 ccgcacaaac tgtatgacca tcaatctcag ctgtgattcc ttgtcccttg attgtctgat
    13981 tatctttgac tgctaattgt tgaaaatcaa tgtctttctc ctgagcataa tggatcacgg
    14041 cgcgccccag aggatgatcg gataacgttt ctaatcgagc cactaaagct aagctgtccc
    14101 cttcatcctc tgtatagttc ttgatagtgg atacagcagt agttcctttg gtcaatgtac
    14161 cagttttatc aaagacaaaa gtatcgattt tggaaaaagt atccattgct tcaccgcctt
    14221 tgactaagat gccccgtttc gctccattac cgattcctgc tacattcgaa acaggcgcac
    14281 caatgactaa agcgccagga caaccaagaa ccaaaacagt gattgctaaa cgaaaatcac
    14341 gactaatcag accaacaacc aacgcaataa gtaaaacggt tggagtataa tactgagaaa
    14401 atcgatcaat gaatttttcc gcatgggatt tggtatcttg tgcttcttct actaattcga
    14461 taattttggc aaaggtcgta tcatctccga ccttagttgc ttcaatcgtc acgtagccat
    14521 ctgctaaaat cgttccagag aagacttcat ctccactgat tttgacgact tgtcgtgatt
    14581 caccagtcac tgcggcttca tccgcatacc ctttcccgtc aatgactcgt ccgtccacag
    14641 gaatagctcc accagttttt actaagacga tatcgccttc ttccacgtca tcgatttcca
    14701 cttcttcagt cgttccatct tcttggacaa cactagcagt agatggggcc atctcggtca
    14761 aagccttgat tgactcccgc gttttggtca atgttttttg ttccagatag ctaccaaaca
    14821 aaaacaagaa tgtaacgata gctgactcct tgtattctcc gatcataaag gcaccaagca
    14881 cagcaatagt aacgagtaaa tcaattgaga taactttgac tttcactgcc tgatacgcat
    14941 ggatcataat aggtatgacc gcaataatag aagctatggc tagagcaatg ttatagaaca
    15001 agacaaaacc agtaaatttt ccgataaaac caaaaataat caacacagct gataaggcgg
    15061 tgatatgatt tcgatgctct tggatccact tttgtaattt catctttctc cctcttttcc
    15121 tttcattctt acgagactaa gtatagatac ttatccatca taaaaaattg accactatca
    15181 agtttagtca aaaaggcggt tttcttttgg tagaatacac tacaaaaaat agttagcaag
    15241 aagactcact aaaagtgatc ttgccaacta ttgtcgtttc tatcgaaatt ttcattgaat
    15301 gattttcctg tacagcatac atcggagatc tatttcttta tctaagattt ttatcttgct
    15361 aattttttcc tttagtcttt caaatccacg tcgttcatag aattgatacg tacagccact
    15421 atctgtaaaa aggaatattt cttttccttt ctctcttttt tcaaattctg ttagcaactt
    15481 ggtaccgatg ccttttgttt tcaaatcagg atttgcagct aaaaaaatga tctctccatc
    15541 tggcgaatgc ttctttaaat atgctgcaag catttgttca tttgctgtct cgtaagctcc
    15601 tttactttct ttagcaaata tattttgtaa aaaatcgaat atcttaacat atatttgctg
    15661 tccaaaagaa cgatgtttct ttttttctcc ttttatctcc gccagcaaaa cacctgccaa
    15721 ctcgtcctct gcatacaaag caagaatttg agttgctcga ttcaactcca tatacaaaaa
    15781 atatcttccg taaagattca gcagaagttc attttccata tatctctcaa agttcattcc
    15841 ttcaacagca aaatagatag cttttttgta atctttcttt tctaagggtt taaaagttag
    15901 tttcatcatt tcgcctcctc aacttcaatg attgtgttat acttacatta taatagacac
    15961 agtgtctact atctataatc aatcatctat aagtttgtcg attaagcgac agctttgctt
    16021 tgatatgtcg gataaggaga taggtaaatg aaaaagacag atagacgcgt caaaaaaaca
    16081 gaaaaagctt tggctgaaac actatccaca ttattagtaa ataaaaagat tcaagcaatc
    16141 actatacgcg aactaacaga aacggcagat gtacatcgtt cgacttttta tacacattat
    16201 aaggatatct atgatctata tgat
//
LOCUS       DAZKOU010000062        16061 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000062 DAZKOU010000000
VERSION     DAZKOU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.063"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..684)
                     /locus_tag="ACG827_001966"
     CDS             complement(37..684)
                     /locus_tag="ACG827_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0484096.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(692..1378)
                     /locus_tag="ACG827_001967"
     CDS             complement(692..1378)
                     /locus_tag="ACG827_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484097.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1384..2457)
                     /locus_tag="ACG827_001968"
     CDS             complement(1384..2457)
                     /locus_tag="ACG827_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0484098.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            2679..3353
                     /locus_tag="ACG827_001969"
     CDS             2679..3353
                     /locus_tag="ACG827_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0484099.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3358..4698
                     /locus_tag="ACG827_001970"
     CDS             3358..4698
                     /locus_tag="ACG827_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0484100.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(4847..5833)
                     /locus_tag="ACG827_001971"
     CDS             complement(4847..5833)
                     /locus_tag="ACG827_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0484101.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            6557..7588
                     /locus_tag="ACG827_001972"
     CDS             6557..7588
                     /locus_tag="ACG827_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0484102.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            7697..9229
                     /locus_tag="ACG827_001973"
     CDS             7697..9229
                     /locus_tag="ACG827_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0484103.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            9368..10180
                     /locus_tag="ACG827_001974"
     CDS             9368..10180
                     /locus_tag="ACG827_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0484104.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            10170..10655
                     /locus_tag="ACG827_001975"
     CDS             10170..10655
                     /locus_tag="ACG827_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0484105.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            10678..11223
                     /locus_tag="ACG827_001976"
     CDS             10678..11223
                     /locus_tag="ACG827_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0484106.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(11296..12939)
                     /locus_tag="ACG827_001977"
     CDS             complement(11296..12939)
                     /locus_tag="ACG827_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0484107.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(13068..15296)
                     /locus_tag="ACG827_001978"
     CDS             complement(13068..15296)
                     /locus_tag="ACG827_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0484108.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(15329..15808)
                     /locus_tag="ACG827_001979"
     CDS             complement(15329..15808)
                     /locus_tag="ACG827_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0484109.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT         4740 a         3062 c         2907 g         5352 t
ORIGIN      
        1 aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca
       61 acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag
      121 atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc
      181 cgacgattga atggagaaat gattgattct taataattcc agccattttt cgttcattgg
      241 agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa
      301 gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt
      361 ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg
      421 gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata
      481 tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg
      541 gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc
      601 gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat
      661 acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca
      721 agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag
      781 taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt
      841 catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct
      901 cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag
      961 gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg
     1021 aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa
     1081 aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc
     1141 gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg
     1201 gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa
     1261 tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct
     1321 ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt
     1381 ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa
     1441 tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa
     1501 aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc
     1561 aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa
     1621 gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat
     1681 cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc
     1741 atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt
     1801 taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac
     1861 gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc
     1921 agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa
     1981 gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt
     2041 atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg
     2101 ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt
     2161 ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc
     2221 ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc
     2281 ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga
     2341 aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc
     2401 gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg
     2461 tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc
     2521 taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc
     2581 tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc
     2641 atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag
     2701 aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa
     2761 cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg
     2821 caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga
     2881 aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga
     2941 ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac
     3001 tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc
     3061 tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag
     3121 ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa
     3181 tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga
     3241 ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg
     3301 ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg
     3361 aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt
     3421 gcttttatct tttttctttt gggattcatt actttacaga tattaaacca atctgcatac
     3481 agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag
     3541 atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga
     3601 acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc
     3661 ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa
     3721 atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat
     3781 gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat
     3841 caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta
     3901 gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt
     3961 atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa
     4021 aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat
     4081 agtttggaac atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc
     4141 gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc
     4201 gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat
     4261 atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt
     4321 ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg
     4381 gttattttgc tggataatgc attaaaatat acgaatccag aagataagat cattttggat
     4441 tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt
     4501 gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa
     4561 gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat
     4621 ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg
     4681 ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca
     4741 gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
     4801 atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat
     4861 tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat
     4921 cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa
     4981 taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat
     5041 cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga
     5101 cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt
     5161 ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta
     5221 gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac
     5281 ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg
     5341 gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc
     5401 cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg
     5461 tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag
     5521 aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat
     5581 aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat
     5641 ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag
     5701 atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca
     5761 accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat
     5821 ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat attgttactt
     5881 ataaatttat tctataggag acgattgttt tctacaaata tttagattgg aatttgatta
     5941 aaacaattta attgaaattt gaaaacattt atgttaatga aagtcatatt ctaataaaat
     6001 gtcatttgtt tctatatgaa tttttatgaa aaatttcata taatttccac tcgttgtgat
     6061 ggtttaattt atccaatcca tcaataagca aacaacttat ataaaaaagg attaattctc
     6121 aatataattg ctcctaccat agaataaatc ttcatgtaaa gtaatgaagt accaactatt
     6181 tctaatactt ctttaagaat tatcccaaca atatcgttag acaaaccatt attgaataaa
     6241 tggagagcca atcgattctg aaaaaatgtt ttctaatttt tcggaaataa aagaaagagc
     6301 ctcctgccat tagaacgaac caaatccatt tcattgctca tgaagtaacc acatagctaa
     6361 aaagaggata aatcaattaa aagatcaaaa gaatgactgc actaataaaa taactaaaaa
     6421 tgtatcttct tacttcatat cttttagcta gtacttagtt aaaaatttgc ttcctaagat
     6481 caagacaaac tgtctatctt catttgatga aatgcggtaa aatatagaaa atgaacacta
     6541 ttggagggaa tttgtaatgg acagcaaaac tagaaaacaa ctgcaagaaa agttaaagca
     6601 acgtacacac ttgctccctt ccgtctttca acagttttta gaagaaaatc gttcttccct
     6661 ttcattaggc agtcgctatg aatacgcaaa agattttcat ctgtttactg attatttgag
     6721 aaaagtattt tctgaagaag tggatgatca gctgattatc catttagaaa aacaagatta
     6781 tcagaatttc ttagctaaaa tctatcgaca tactcgttcg ttccaaacta cagcgaacca
     6841 agaaacggag caattgtttg aaaatagcta ttacgggatt tcaaggaagc aatattcatt
     6901 aaatcatttt ttacgatttt tgtatcaaaa aggactgatt gaagaagaga tttctctttt
     6961 gggtacaagt ttaccggaaa caaccaaagc agctccgcga tatatcgatg cagaactttt
     7021 caaagatttt gagcatttgt ttatcccgat tgaaggcttt caaacaagca gagaagctag
     7081 ctttgaagaa aagaatagac cgaggaacct tgcaattact gccattcttc tgtattgcgg
     7141 attaaagatc catgaagtag tagaacttaa acgtaaagat gtatctttga ccaagcagat
     7201 tcttaatctg aatagaaaag aaaacagact gaataaagta ccaataccaa aagaagcgat
     7261 gccatttctg gagaactatt ttaagttaac agcacaaaat acggacgaaa attcttttgt
     7321 ttttcgttcc tcacatgatc agcaaatttc tcctcggact attcgccaaa ttttgagcaa
     7381 aattacagta tataaaaacg tatcgccaga actatgcaga aaaacatatc gttattatgc
     7441 agaactatat ttagatgatg ctgacgcggt aaattattta tgtggtaatc gatacctctc
     7501 atcccattct tttcaagata tctgggaaaa gatggttgat ttttcttatc acgaattagc
     7561 ggcattcgtt cgggtttctt ccatataaaa caaatgtatg atcttttttt cacaataaac
     7621 tatgttatta tataaaggat tatgttacga ttatcttgaa tttgtatatt acttaacaaa
     7681 agataatgga gtgtgaatga tggagaccat aacgtattcc cctttgaact tatttacgaa
     7741 ttttcaagaa gctgcagcta acttttccga gaccccgatt atctttgatg agccactttc
     7801 agctttccca gaattaggac ttgaaacaac ttacaaaaaa tgctgcgaaa gtgtgttgaa
     7861 acgggcttat caattggctc atcttggagt gaaatccgga gacaaagtta ttatttataa
     7921 gagttccgct tttgacacat accttctagc tgtttctgtg tcttaccttg gtgcagtgcc
     7981 tgtcatgact tcttatcatc taccaacaac aacaatggaa gtatttatcg atcgtttaga
     8041 agaccctttc attttatttg atgatgagac aaaaaagcga gtgagagaaa tttcaaatgg
     8101 aacaaaatca aaacagattc ctattcttta tttaatggaa caacctgctt tgtctgtttc
     8161 tcaatcattt ttagacaaaa atgaaatttc ttatatgacc catacttctg gtacaactgg
     8221 tatccctaaa cttatttgtc attctgctca ttctatggga tggcggacga aatggcaaaa
     8281 aacagttttc acaaaaattt cggaaaagaa attaatcgct tttcacatct ctccagtgca
     8341 ttccagattc aatattggta tctcttcttt gatgtccatg ggatttccta tgatgccttt
     8401 agcaaacgca caaagttcaa aagttgttca tatgttggaa gctcaccggc caattgcact
     8461 agagacccat ccgaataact ttgttcagtg gcgttttact gctaaggagc atccagaagc
     8521 tttttcgggt atccgatact atcactccac ttttgatgct ataaacaacc agacaatgaa
     8581 gacatttttg cgaacgtcga tggcacagga tgcggtattt cttcaagtat atggccaaag
     8641 tgaatgcgga ccaatgattc ttaaggctca tacactagag agtttgaaaa caagtgatgc
     8701 aagagatatg ggcgttggac tggaagacat gacaagcgct cggattacag attcagttgg
     8761 gaatgtccta cctgaaaaca cagatggtca tatacaatta ctgtctaaag gacgggcttt
     8821 gacttattac aaggaagatg cacggttcca ggaaaatgtt tatggcgatt ggtgggatag
     8881 cggagattac ggttttatgg ataagcatgg ccacctattt ttgaaagatc ggcaagtgga
     8941 tttgatcgaa acgatcaata gtacattagc tattgaagac tttttattag attcgttaga
     9001 ttttttggca gaagtagtga ttgtaaggga taaaaatcat tctccacaac cgattattgc
     9061 tttagctcct gataaagaaa tggattggaa tcgatggtgg gagcaagtcc atgaacttcc
     9121 tcgcttgaac gtccctatca tccgtgattt tgatgcaatc ccgcgaacag ctacaatgaa
     9181 agtgcaacgg cttcaaatcg agaaagaatt aaaatcacaa aagtcctaac tatttaagga
     9241 aagaaagatc cattttggtc tttcttttct ttttgttttt ttaaattgga tgagataaaa
     9301 atatgccaat tggctgtttt tcttttttga aaatttcgtt atactcttat tcatggagga
     9361 aaatgttatg aaaagaatct tgacaattgg cggttctgat ccatttgctg gtggaggaat
     9421 ccaaacagat ttaaaaactt ttgagaattt tggtctattc ggactaagcg ccttgacttc
     9481 tataggggca ctagatatta atgagcgatt tatgctggaa tctatatctt tggaattgct
     9541 taaacgacag ttagtttcga ttgataaaat gacttcatta gatggtatca aaattgggtt
     9601 attgaattca agtgaagcaa tcatggttgt tcgagacttc ataaaaacaa aacaaaatat
     9661 cccaatcatt ttagaccctg ttttggcatt caaagaaact gaagctgtga tcaatcaatt
     9721 gtatatttcg cagctcatcg aagaactttt tccacttgtt acgatgatta cgcctaattt
     9781 ggcagaagct gctttacttt ctggtcaagc tcttccctct tcgttaacag aaatgatcca
     9841 tctcgctaaa gaactcactg cttcaggaac aaaaaacatt gtgatcaaag gcggcacaag
     9901 gatggctgga gatgaagctg tggatcttct ctattcaatt gatcattatg aggtattttc
     9961 tcataaaaaa atcgacacta caacagtaaa cggggccggt tgtgcctttt cttctgcaat
    10021 aacagcgaat cttgtgttag gggcagattt taccaaagca gtagcagaaa gtaaaaaatt
    10081 tgttgcccat tgtatttcta atggcgtgct gatgaacgat cataccggaa gtgtctggtc
    10141 tggcggtatt caaaaaggag gaatgagaaa tgaaaactaa gcaattaacg atctatgcta
    10201 tgctgattgc gttgacagtt gccctttcat taacaatttt gatccctgta ccggctacga
    10261 atggatttgt gacattatgc gaggccggga tctataccgc tgcttttctt tatggtccaa
    10321 cgggtggatt agttgttggt gcttccagtg gtctattgat cgatttgatt tcaggatatc
    10381 cgcagtgggc agttttttca ttagtgatcc acgggttaca aggatatgtt gctggttctt
    10441 tcagcaaatc ttctactcgt atgtggggat tagggctttt attaggaagc ttagttatga
    10501 tcatcggcta tggtatcgcc ggttggattt tatatgattg gccggctgga gtggcttcta
    10561 taccaggaaa catcttccaa aatctgattg gaatgggact tgcctttcct ataactgcaa
    10621 gtcttcaacg tattcaagca cggaagcaat attaattgaa aaagaaggtt ttagaaaatg
    10681 aagttgtcag aaaaagaatt aaaagaccaa ttgactgaaa tagttaatga catattggct
    10741 gaggcacact tgaaaaaagg tgatcttttt gttttaggat gcagcacaag cgaagtcgtt
    10801 ggaggacata ttggaaaaaa ttccagtgcg gaagttggtc aatggatcat ccgtacgtta
    10861 aaagagttgt tggatcctaa agaaattgcg ctagctgtcc aaggttgtga acatttgaac
    10921 cgagcattag tagtagaacg aacagtggca gaagcaaaaa atttcgaaat tgtctctgtt
    10981 gttcctgcac tacatgctgg aggtgcttgt tctgttgctg cttttgacca gttcaatgat
    11041 cctgtcgaag tggaacacgt agttggacaa gccggtatcg atatcggtga cacatcaatc
    11101 ggtatgcata tcaagcatgt ccaagtacct gtcagacctc gtttgaaaac ccttggacaa
    11161 gctcacgtga cagcccttcg ctctcgtcct aaatatatcg gtggtcctag agcgaattat
    11221 tgaaatgagt aattgacaga gcaatctgaa aaacttcctc gcaaagtcca actaatgaac
    11281 tttaacgagg aagttttact tgtttgtttt atataaccga gcttcataag gtgctagtat
    11341 gcctttagct tcaaattctc cgctagagga atttgacaag agtaatgtcc aggactgatc
    11401 tgcttctttt ggaagttcat agtcagcaag tgattctgtt aagtttacca taataagata
    11461 ttgttcatct tctaaatatc tttcatagac ataaagttgt ggatgttcat gaagatattc
    11521 tttataagaa ccgtatatca gacctttatt ggtttgtcta atgcggatca tgttcttata
    11581 gaattgcagg accgactctg gttcatattc ttgttgttct acattgatcg ttttggtatt
    11641 tggattagtg atcagccaag gttcatgtgt agaaaaacca gcatattgtt ccgaagtcca
    11701 ttgcatgggt gtcctgctgt tgtctctcgt tgtttcacga atgatatcca atgcttcttc
    11761 tcttgtctta ccttccgcaa gcagcctttt ataaagtctt ttcgaatcta ctgcatcaac
    11821 ttgatcgatc gattcaaagg gcatattcgt cattccgatt tcctgtcctt gataaataaa
    11881 tggcgtacct tgcaaaaaga aatacatcat agcgattgct tttgcagaca tagcccaaaa
    11941 atcgggacga gtatctccaa acactgaaac ggcacgagga acatcatggt tttccatata
    12001 taaagcgttc catccgatac catccaatga agtttgccaa gcgcttaatg catgtttcaa
    12061 tttaaggaca tctaattgcc cttctttttc ttgctgccag atatggatat gatcaaattc
    12121 gaatatcata tcgaaataac cgtctttccc tacccattgt ggtccttctt ctgcagtgac
    12181 accacttgct tctccgacag tcatgatatc gtactcttca aaaacatgtt tcaaatcggt
    12241 caaatgttct tcgattcctg agacattttg aaagggtgag aaagggttct ctgtggcttt
    12301 tactgaatat tcatcttttt ttacatgaga aatcgcatct aaacgaaatc catctatccc
    12361 tttatccaac caccatcgga tcatattgaa tagttcttct ttcaatttct cgctttccca
    12421 atttaagtct ggttgttctt ttgcaaaaac atgcagataa tactgtccag tttcatgaga
    12481 atactcccaa gcagaaccgc cgaaaatcga tgtccaatta ttcggttctt tgccatctac
    12541 cccgtcgacc caaatgtaat aatcgcgata cgggctttct ttagattttt tcgattcttc
    12601 aaaccattga tgctgatccg aactatggtt gatgacaaga tccataatca ctttgatccc
    12661 taattcatgt gcttctttta gcaataaatc aaaatcttcc atcgttccga atctctcttc
    12721 tatatcttga tagtcactta cgtcataacc attatcgaca aatggagatt gatagatcgg
    12781 attgatccag ataaagccga taccaagatc tttcaaataa ttaagttttt cacggatccc
    12841 attgatgtct ccttgaccat cgccattgct atctttgaaa ctagcaggat agatttgata
    12901 gcctactgct tctttccacc atttttgttc ttgtatcata tcgtttcact cctttaacgg
    12961 tccctcttga ttataaccga ttctatttta gaagtatcct tgttaccggt aactagaaac
    13021 gaaaagctca acgaatctgc taagtgcgtt cgttgagctt tattttatca tagatagcgg
    13081 gcaatcagtt gaatcatttg cttgggaaat tcattcaagt cagtaatatc tacaaaacga
    13141 ctttcaccgt agatcgactt gatttcttct ttgtcttggc caatagctgc ggagacaaat
    13201 aggattcctt gacgttcata ttctttcaaa acagactgga tatcttcttt tgcttttttg
    13261 cctgtataat ccggcaacgc ttttggctga ccgtcactaa tattaagcaa cagtttggtt
    13321 gttgcggatt gtctgtttaa tttttctgct gctaaacgaa gtgcagcacc atcacgattg
    13381 ttttgccttg gtttcatagt gactaatttt tcatttaacc attgaaatcc gtcttcaaat
    13441 tctttataag aaaacaaaga agtcttttct cttgacgaac ggtctgctgt atctccgtaa
    13501 atcaacaatg ggatatcgac tttcttagaa aattcggcaa tggcaatcgc tgcttttttt
    13561 gccgcttcga tccgatcatc ccgtatcatt gacccagatt catcgatccg tacagctacc
    13621 gctaaagaag gttgctcatg tggcgggttc tttttgtcaa aatttctcaa atcaccatat
    13681 gctactttgc ttgcattgaa ccgctgacct tcgtattttc ctttttggta cgtctctgtt
    13741 tgttcattat ccagtaactc taaaatttgc cggctaagtg attctactat tggcattact
    13801 tgcatgtgta gctcacgtgc ttccggcgtt tttccttgga aagctggtcg gtgaacgatg
    13861 agaccttctt tttcatgaat cgtttccttc atgatttgtc gcgcttcttt attcagttgc
    13921 ttcttgattt ccttatcatg tgcttccatg tcagaagcag acatttcttg agaatcatct
    13981 cctgtttgtt gttctttttg attttttcct tcgatatcgc tactgcgttc tttttgcatt
    14041 tgaggaggaa tctcgttttt ttctattgtt tcttcttgtt tctcttctcc ctcatttggt
    14101 tcttcagcag acaacgattt cttttcatct aagcgcagaa tttcttcagc attttcagtg
    14161 atgtttcgtt catttgcttc ttcggcgcta atggttcttt cttttgtatg ctcagattga
    14221 ggatgacccc atttttcttt tctcaatatg tcctgaagtt ccagtagcaa tccactgtca
    14281 gacaaagctt gttcaagaat tgccaattca tctgaatcag tggataattt atacaaaact
    14341 tttggataaa tcgactggag taaatcttgt ccttgcttat aggtatttgc ccaataaaaa
    14401 taagaacgca ttcctgctac acctttgatg gcgttagctt ttgcagtttc atccaacaaa
    14461 cggataatct ctgccatctt taagagaagt tcttgttttt ggatcatagt tttcgccatc
    14521 gcacgttctg ccatgacttc taaggcagga agatccattt tttccgcatg ttgcatccga
    14581 tctcttaaag attcatttaa tgggcgattt ccttggtaat ttcgatttgt agtgatcaca
    14641 atgacgcaat ctggatggcg acgaatgatt cctgtcggta aattcaacat cccatttggt
    14701 tcgagcgctg agttaagtgc cacaagaaca gaggcatctc ggatgacagt cggctcttga
    14761 atctctaata aatagccttt ttcaatcgct cgaacgattt cagaagggta atagctgtaa
    14821 tggattgcat ctggataatc agcttcgatc ctttgaacta gctctgctaa tttttcttta
    14881 gcagaaaaag gatcaatgcc gtaatggttc tggatcagtg tcagtacagc ttcaagtgta
    14941 tctgtttggt aaatagcttc tagtaattct tggtcttgtt ccttattaga atctactaca
    15001 ggtaataagg ctccgaaaac atctgatttg tccatatctg caaaacaggt cacttttgta
    15061 tagggaagtt gcagatcagc agatagtgct ttggctaatt gtgtcttccc cgacccggca
    15121 tctccttcca aaagtacgtt actgattttc atttcaggat cttgccaatt ccctttgatt
    15181 tcggaggcga tccgccattc ttctgcactt gtccgatggc ttttcggttt cttccagatc
    15241 atttgctttt ctagccgtga aaaggttcgg ttttctgtta gtgagaaatt ctccatgctt
    15301 ctcttcccct tttgatcatc tctgtctatt aatatcctag ctcatctaaa agacgtttta
    15361 aaatacggaa tcgttcccca atcagttctc cgtctttttg tagtgtatcg taatacaaaa
    15421 tgatgtcttc atcgatcttt ggatttttgg tatctacttc taccgtcatt cggttccctt
    15481 gtaatccaat gatgtctacg actggatttt ctggatcata aaatttttca taacgataag
    15541 catcttggaa gtataaactt ccttgcgcag ctaagatcgc taaatctaaa atacgtgtga
    15601 ttggtaattc ttccgactgt cgtgaccatt tttctccagt atgtcgccat accttgcctg
    15661 agatatcgac ttttccacga tcattccact gagcaaggcc cagtgaaagc ccttttgcat
    15721 ccatatttaa tgcatttctt ccatcaactt tatcatagtc ttctacaacg attactggtt
    15781 tatgttttaa atttgttggt atttccatta ttttaactcc tcatcctttt atttttagta
    15841 aataagtaaa ttagtagttt actcatttac taaactatat gcgacattat agaggattga
    15901 agtgaggatt gcaaagaaaa agagcttcat tgatagaaat aaaaacacca tctcgttctt
    15961 ttacacgctc attagtaaca tgtagaaagt ccaagatgat gttggaatat tttggctcta
    16021 tactttttga tgttggtgaa cttcaccaac atcttttttg t
//
LOCUS       DAZKOU010000063        15938 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000063 DAZKOU010000000
VERSION     DAZKOU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.064"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..1490
                     /locus_tag="ACG827_001980"
     CDS             66..1490
                     /locus_tag="ACG827_001980"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0484110.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1789..2496
                     /locus_tag="ACG827_001981"
     CDS             1789..2496
                     /locus_tag="ACG827_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0484111.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2483..2806
                     /locus_tag="ACG827_001982"
     CDS             2483..2806
                     /locus_tag="ACG827_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0484112.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2895..3932)
                     /locus_tag="ACG827_001983"
     CDS             complement(2895..3932)
                     /locus_tag="ACG827_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0484113.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4253..5236)
                     /locus_tag="ACG827_001984"
     CDS             complement(4253..5236)
                     /locus_tag="ACG827_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0484114.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5651..6421)
                     /locus_tag="ACG827_001985"
     CDS             complement(5651..6421)
                     /locus_tag="ACG827_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0484115.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6414..7349)
                     /locus_tag="ACG827_001986"
     CDS             complement(6414..7349)
                     /locus_tag="ACG827_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484116.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7665..9314)
                     /locus_tag="ACG827_001987"
     CDS             complement(7665..9314)
                     /locus_tag="ACG827_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0484117.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(9431..12034)
                     /locus_tag="ACG827_001988"
     CDS             complement(9431..12034)
                     /locus_tag="ACG827_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0484118.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12046..13095)
                     /locus_tag="ACG827_001989"
     CDS             complement(12046..13095)
                     /locus_tag="ACG827_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0484119.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13121..13435)
                     /locus_tag="ACG827_001990"
     CDS             complement(13121..13435)
                     /locus_tag="ACG827_001990"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0484120.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(13451..13897)
                     /locus_tag="ACG827_001991"
     CDS             complement(13451..13897)
                     /locus_tag="ACG827_001991"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0484121.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(13898..15820)
                     /locus_tag="ACG827_001992"
     CDS             complement(13898..15820)
                     /locus_tag="ACG827_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0484122.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         4706 a         3240 c         2654 g         5338 t
ORIGIN      
        1 caaaactatt tactttaagt tgtaagcggt gttatcatta agtggaaaca ggaggtaaaa
       61 aaattatgac aactatgaaa aagctaactt ttccagatga tttttggtgg ggttccgctt
      121 ggtcagctga gcaagcagaa ggaaaaggag aaacaggaaa agcagagact atctgggatc
      181 gctggtttaa agaacaacct taccggtttt atcaacgaat cgattcctct attacaaccg
      241 atcacatcca tcaatataaa gaagatatag aactaatgaa aaaaactggg cataactcct
      301 ttcgcgtttc tatttcttgg gcacgaatgt ttccaaatgg cggtaccgga aaagtaaatc
      361 ccaaagccat ttctttctat cgaaaattat tttccgaaat gaataataac ggaatcaaag
      421 tatttgccaa cttatatcat tttgacatgc ctgctactct tcaagataaa ggaggatggg
      481 aatcacgtga agtagttgat gcttatgttc attacgctga aacttgtttt aaagaatttg
      541 gtgacttagt ttgtcattgg tttaccttca atgaaccact tgggcctatt ttaggtactt
      601 atcttgaaga ttttcattat ccaaacctca ttgactttaa acgcggagcg caagcagcat
      661 tcttcactat acttgcacac gcaaaagcaa tacaagtatt taaagaacta gctcttccta
      721 gtaaaatagg cgttattcta aatttgagtc ctacttttcc tcgtagtcaa aatcctgctg
      781 atgtgaaagc agcagaaatc gctgatttat tttatacgcg gagcttttta gatcctatgg
      841 ttaaagggaa tttttctaaa aagcttatcc tgttattaca agaatataat cagcttcctg
      901 aagattatac gaaagaagat cttgtatgta tctacgaaaa taccgctcaa attcttggac
      961 taaactatta cgagccacgt cgcgtcaaat ctcgttcttc tatggtggat acatctagtc
     1021 catttttacc agagtggttt ttcgaaccac atatcatgcc tggacgacga atgaacgaat
     1081 accggggatg ggaaatttac gaaaaaggaa tttatgattt gtgcatggat atcaagaacc
     1141 attatggaaa catcgaagca tttatctcgg agaacggaat gggcgttgcg gatgaagaac
     1201 gtttcatgga tgatacaggg caaataattg atgattatcg gattgagtat atcaaagatc
     1261 atcttgctta cttatggaag gcaataaatg aaggatgtaa cattaagggg tatcatcttt
     1321 ggacatttat tgattgctgg tcatggatca acgcctataa aaaccgatat gggctggttt
     1381 cactcgacct tactactcaa aaaagaacaa ttaaaaaaag tggcgaattt tacaagcaat
     1441 taagtgatac aaatggattt agatacgatg tagacttgtt agttgaataa tattctaaaa
     1501 ttccacttga agtgaacaca ttagaaaaca agaataatga gaaataccaa taacaaatac
     1561 tttttcttca tacttcattt atacttgtca gtataagaga ctgtgacaaa tgtagtgatc
     1621 ctcagaagtt aggtctggaa atctaacttg agaagatttt tttaaactac gtccagtctt
     1681 ttttatttgt ttaaaattga agttgacaag tcttcattaa aaatttagaa tattcacaag
     1741 tttgacgaca attatgaaaa tttatgtcgt gctaaaggag ttttgttatt ggatgaaaag
     1801 ttagatataa aaactgcgat caaagacacg ttacctaccg tttttggtta tatcggtatt
     1861 ggacttgcat ttggtatcgt tgggaaagct gccgggtttc atccgctggt cgtcacgttg
     1921 atgtccctgc tgatctatgc tggttctgcc caatttatca cggtcagcat gcttgctagc
     1981 catagtccac tgctctccat cgttctctcg acctttctag tcaattcccg aatgattctg
     2041 atgagtatga cgattgctcc ttatttcaag aaaaatcggc tgcttcaaaa tctgctgatt
     2101 ggcacattgc tgacagatga aagtttcgca ctgggaatga ataaattgaa ctatactgga
     2161 caaaagttaa actttcgttg gatgaataca gctaattgga tttcttattt gacgtgggtg
     2221 ggttcttctc ttgttggcgc acttttaggg aattttatta ctgatccgaa aaagtttggt
     2281 ttggatttcg ccatcgtcgc tatgtttatc gggttacttt atcttcaagt catttctgat
     2341 agaaacacct ctaaacgatt acagttgatc ctgattggtc tcactttgat tctggtatat
     2401 gtcggactga tttttatccc aagtaactta gtgattttag ttgtaacact gattgggtgc
     2461 ggattggggg tgtggatcaa acatgccttc ttttgagttt acattgctga ctattttagg
     2521 ttgtacttta gcaacttggc tttctaggat cctaccattt gtcttactga aaaaattcga
     2581 tttgccgcaa ccgttgcttg agtatttaag ttttgtcccg atcgtcatta tgtctgcttt
     2641 atggttcagc agtctattta cccaaaatat cggacatctg ccgcagatca atctcgaaaa
     2701 tgctttggct tcagttccta cacttttagc tgctattctt tcaaaaagct tattggtgat
     2761 cgtcttggta ggaattcttt ccctttcgtt gattcgctta ttataaataa aaaaacaaga
     2821 gcttatggca aattactgtc atgcgctctt gtttttatca aagtcaaata agaaaaaaat
     2881 cggcgtattc ttgcttatcc gttgaacgga ccactaacaa cgacttttcc taatatatgt
     2941 cctgtttcta cgtctttggt tgcttgtttc aagttcttcg cattcatccc atctgaatag
     3001 accttgctca atgtactgcg gataatccct tcatttgcta aagcagcaat gtgcgctaaa
     3061 gcttctcctt gcgtttcgat ttcatagtta tagtctgttt tagcgaacat atactcccag
     3121 tcgaagctga tagattggtt tttccaaaca cctatatcta acggctcttt gatccctacg
     3181 atcgttccca catgtccaaa tggtcggaca aggtgtttga tttgatcaaa gtagcgggta
     3241 atatcgaata atacagcgac atactctact tgatcgattc ctaattcacg aaaactttta
     3301 cttaagtcgt gatgataatc gattggatag tctacacctg tttttttcag ccattcaaaa
     3361 ttcttcggac ttgcagtcgc gtagacttct aggccagccc aatgcgctag ttgattcaaa
     3421 atcgaaccga cgcccccagc tccgttgatc actaagattt ttttcccgcg gttggcattt
     3481 tcttctggag tcagaccaaa tttttcaaat aacaattcat aagcggtcaa tgaggtcaac
     3541 ggcaaggctg cggcttcttc atctgaaaga ttttttggtg caagagcgac aatacgttcg
     3601 tcaaccagtt ggtattcttg attgcttcct gctcttgtcg ttgtacctgc gtaaaaaacg
     3661 cgatcgccga cagaaaattt ttgcacttca tcacctacag caacaacttt cccgactcca
     3721 tcaaacccta agatacgtaa agcatttctt atcccgttat cttggcgaag tttcgtatcg
     3781 acaggattaa cagatactgc gttgactttt accagaagat cccgtgaact gggtacaggt
     3841 atctgttctt tttcatcaat aaagcttttt ggatcatcga tcgacaaccc ttcataaaaa
     3901 ccgactacct tcattaattc cttttctgac atacgaattc ctccttagct tcatcaagtt
     3961 ggagcatcca tacagttttc atttcaatat atatagtatg ctatttgttc cacttttatt
     4021 catcataatt attctcttac ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg
     4081 ttgcgacaag acttttgtca caacctcttt taaacggtgt tcaatcaact gaccctcggt
     4141 attcgctcaa aaaacgacaa aatatgagga gctatttttg tttggctgtt cttattactt
     4201 gatgcaggac agctttttca cgacctctaa ttcacggtgt tcaagcaaga aatcattgtt
     4261 cactgaaaag cgttggatgt tctttttcaa aggtgtctaa gatctctcgt acttcatcag
     4321 tagatttcgt attcatcaac tgaactcgca agtcatttgc tcctggaaat cctttgatat
     4381 agattttaaa gaaacgatgt agtcctgtga tggaacgcgg gatgatttct gaataatgat
     4441 cttgcagatc tagttgcaga cgaagtagtc cgatcagttc tttttctgta tgttccctcg
     4501 gttctttttc aaatgcataa ggattcttga aaatcccccg gccaatcatg atgccatcta
     4561 cgccatattt ttcggccaat tcttctcctg tctggcgatc tgggatatct ccgttgatcg
     4621 tgatcagtgt ttgcggagcg atttggtcac gtaaagcaat gattttaggg atgacttccc
     4681 aatgtgcatc tactttactc atctctttac gcgtacgcaa atggacagaa agattggcta
     4741 tatcttgatg aagtaggtga ctgatccatt cttccatctc agacatttcc gtatagccga
     4801 tacgcgtctt gacgctgaca ggcaaaccgc cagctttagc cgcttcaatc aattgtgccg
     4861 ctacttctgg gcgaaggatc aagccactgc cttttccacg ttctgctaca ttagggactg
     4921 ggcagcccat gttgatatcg attccttgga agcccatttc tgccatagca atgctcattt
     4981 ctctaaaaaa ttctggcttg tctccccaaa tatgggcaac catcggctgt tcatcttctg
     5041 taaaggtcaa tcttcctcgt acactgtctt ttccatctgg atgacagaaa ctgtctgagt
     5101 ttgtaaactc agtaaagaag acatccggtg ctgctgcatg ctttacgaca tggcgaaata
     5161 caacatctgt tacatcttcc attggtgcta aaacaaaaaa aggctttggc agctctttcc
     5221 aaaaattagt agtcatttat ttgttactct ccattctatc aagcagtgaa gtcgcaatct
     5281 ttctcaaaaa cattgtcatt atactaggaa acaaaaaaag aagcaaagtg aacagcttaa
     5341 aagatgtttc tgattgagca aaaaaagact gtgacaaaaa tcttatcaca attccttttc
     5401 cggaataaat ggtgttcaaa agcttgcatc tgcggtaata agtccaacca aacgaaaaat
     5461 aggaggagct atttttgttt ggctgttctt attacttgat gcagaacagc tttttcacaa
     5521 cctctccact aaacggtgtt caatcagctg accctcggta ttagctcaac aaacgacaaa
     5581 atatgaggaa ctattttccc gtttgtttct ctaatactca ggtcaaaacg ctgatttcac
     5641 aacctctttt ctaacttctc aatcctcttc ctttattgat caaataccaa gtaacggcgt
     5701 acaaaacaac gatgaatagc acaagtacga tcatcgatgt tgctactggc acatcgattg
     5761 ttccaaggaa tccgtaacgg aaaccggaga tcatataaac gatcggattg actttcgaaa
     5821 cagcttgcca aaatggcggc aacatagaga tggcatagaa aacgccacct agataagtta
     5881 acggctgcaa gacaaatgta ggcacgatcg atacatcgtc ataagactga gcaaaaatcc
     5941 cgttgagcag tcctgctaac gaaaacaaga ttgccgtcat caataatgta atgatgacca
     6001 tgaaccatga atacacatga agtggtacaa agaacaagga aatgatcgtt acgagagagc
     6061 cgacgagaat actgcgaccc actccgccaa tcacaaagcc ccaaatgatc acatgcgtag
     6121 gaacaggcgc taccaagatt tcttcgatgt ttttctggaa tttttgagag aagaaggacg
     6181 aagaaacgtt ggcatacgaa ctagtgatag cagacatcat aatcaaacca ggcacgatga
     6241 attccatata tgaaaaacca cccatgtcac cgatccgacc accgatcatt tttccgaaaa
     6301 taacaaagta caaggatgtc gtgatcacag gcggaaccag tgtttggacc cagatacgca
     6361 aatagcggtt cgtttccttg atagctaaac tttttaatgc agtaaaataa agattaaaca
     6421 tttttctcct ccgtatgatt attttcttct gtgattttca aaaacagttc ttctaaacga
     6481 ttcgacttat tcctcataga aagaactttg attccttgtc ttgtcagctg atcgaagatt
     6541 ccgttcaccc cttgatttcg ttcaacttcg acagcgattg tctgttcatc ttcaaatgta
     6601 caagcgtatc cttcaatcac aggtttggtc tcatatggcg ctaaatcaaa gataaatgtt
     6661 tcaaattgca gtttggatag aagctctttc atactagtat tctcgatcag ttcacctgat
     6721 tggatgatcc cgatgttgcg acaaagcatt tcagcttctt ctaaataatg cgtcgtcaaa
     6781 atgatcgttg taccattttc gttcaattct cggagaaact cccacatctc gcgtctcaat
     6841 tcaatatcga ctcctgctgt tggttcatcc aagatcagca aacgtggttc atgcatcaat
     6901 gccctagcaa tcatcagtct tcgcttcatt cccccggaaa gcatacgtgc acggacattt
     6961 ctcttttccc aaagattgga ctgcttcaaa tatttttcgc tgcgtttcat cgcttctttg
     7021 cgagagacac cgtagtagcc tgcttgattc acgacgattt gctggaccgt ttcaaaggga
     7081 ttgaaattga actcttgcgg gactaatcca atctgttgct ttgctcggac tagatccgta
     7141 tccagatcat aaccgaagac attcactttt ccagatgttt tattgactaa agaagtaatg
     7201 attccgatcg ttgtagactt cccagctcca tttggtccta atagcgcata gaaatctcct
     7261 tctttcacgg aaagatcgat tcctcgcaat gcttcgaccc cacttccata aacctttttc
     7321 aaattattta tttcaagcgc atttgccatt tatttcctat ctccttatag acatgattct
     7381 ttattgtatc atggaataca gcagatgaaa aaacaacttg gttccagcga aaaatcaggt
     7441 tgctgggctc aggaaaatct cctaatgaaa gacatcaatc ggacgaagtc agacaacctt
     7501 ttcagaaaaa acaaatcgaa gataaaggat tactattcat atgtgcaaca aaaggagcgt
     7561 gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
     7621 aaagccaaga tttttgatcc aataaatact ttcgatcact tcgcctatcc caatacatgc
     7681 cactgccgct tttcatcaaa taacgcggct tccgtttttg cggtttctac taaagtctct
     7741 gggtaatcca aaactttcag caaggcaatc gcatttcggg tccttgaagg tccttctttt
     7801 aatttgaaat caaaactgat tccttttcct tctgttactt gttcttcaaa gtggacattc
     7861 tcacaataat tctttaaaat ctctgtcaat tcgatatcat gtgtggcgac aaatgctaaa
     7921 gatggatact ccgcaagcca ccgaacgact gaagcggaag aagcaatccg ttcgatcgta
     7981 tttgtccctt ttaaaatctc atctacgaag cagtagcagc gttctttgga agccacttgg
     8041 tccaacaaac gtttgatcga tttgatttct gaaacaaaat aactgtcgcc ttcaaataaa
     8101 tcatcttcga ttgccatgga agtcaacaca tgtccaggtt gcagggtaaa ctgttctgca
     8161 atcgctgttt ggatcgtctg tgctagaata cagctgatgg ctatactttt gacatacgta
     8221 gattttcctg atgcattgga acccgtgact aatgtattct tctgccagtt gaccggattc
     8281 actaccgctt cttttaacaa tggatgatag atatctttcg ctgtcacacc gccctcagta
     8341 aactctggtc ggcaagtgat gggcatataa gtacggtaat tcaatattgc agcggctact
     8401 tctaattctc ccagcaattc ccacagcttg atggcttcag ttgaatgctt ttctaattta
     8461 gtgagaacga aatgatagga gataaatggc agcatgaaca tgatgttgac atattcaaaa
     8521 atcaactctg cttcactccc actcttcata cgaaatgaaa aacctaattt agggatgtct
     8581 cttaatggtt tagtttgctg tttcagctct tcttgaagtg gtgtcggcag ttttgctagc
     8641 tgattggcgg ctgaaatcgt tgaaaccaaa taactcatgc tattcagctc tgtttctagt
     8701 gtttgtttct tcactagata atagaccgta ttgaatacta acccaccgat tgccaaatag
     8761 attcctagta cttgtcctaa aagaagcaaa cccaaaccca ccagcggaag caagccaaga
     8821 aaaataaata aacctacgcg tccaatcttt ttactttttc catccgctag atattgtttt
     8881 gcaaaattat gatctttttt tcctaaacga gcgaattgat attggatttt ttctctcagt
     8941 tgaggatttt cctgataaaa agcaatcagt ttttctaact gctgatcttc accaaaatca
     9001 aagctgcgta aacgttgata caacgcttct gaacctacac tagaataggt actgttgatt
     9061 ccttcgaata cttcgaacat atccaggtca taccaagtaa gatcatcgat ttcgctatcc
     9121 catgaacgga acttcttctc cgtgagccat gcttccttca agctttcttc tttatcaaat
     9181 ctcggttgat aaggaatttt ttcccatttg ctacgaactg tttcctttaa tttcaaacgt
     9241 gaaaaaatgt ctaaagcaac taataaaata acagctgcaa taataattaa aacgataaat
     9301 tctctttgac ccatatccct ctccgttcct ttatttgtta gctttagtat agcactgttg
     9361 cgctatttga aaagagggta aaaaaacacc tttcaaaata aaaggtgttt ttttcttgta
     9421 tattacatat ttatctctca tttatcaaaa ttgacaaata atcataggga ggaattgttg
     9481 ttaattcttg tttattctcc agtgcattga caactgtccc aagggaaagg atagcctcag
     9541 gtactatttt ttcttgttct gttggattag ctaagcgaag aacaaatcct tgttcatgaa
     9601 aataagaagg ataaatcgat gatacaatta agtcttcagg taattcaatc aatgaaaatt
     9661 tacgaggaac atcaagttca ttttgtaatg gccaaatctt gttatctaac ctgtgaataa
     9721 atacattcag tttctgcttt tgataagaaa tagattgttc taaccgtttt tctgcctgtt
     9781 ttgccacttt gaactcatta aatcttcctt cttctacatt aaatccaaaa gtgactttat
     9841 actcacctat ctcttgagct agcggtgttt ccatcatgac atgcccttca tttgtcgtat
     9901 ctcctgaagc tcgcccaggt cgccaagcaa gattaggttt tccaagttcc cctgttgttg
     9961 aaaataaggt tatgattaat tgattttcta tacgttcata ctcttttaaa ccatctgcat
    10021 aaaacgtaaa ataatgatca ttttctgcca cagagacact cttatcgaat atttctaaat
    10081 taacaggttt ctctaccata gtttctgacc aattcggagg aatgacatca ggggtatttt
    10141 caataaatcc gttttgaatt tgtgcaattg atttttcaga tttatctaaa acattaattg
    10201 ctaagcgcat acgatgcgag taaacagtat tatgaatttt gagtgtgcct tcaacgattt
    10261 gactttctcc cagactgaac gatatttcat aagatatttc ttccatttcg ggtttttcag
    10321 ataaacgatc ttttaaatct ttaggaagtt gagcttttcc gtaaacaact aatgtttctt
    10381 gtaaagaatc tttatatact ttagctgttt ctaattttag agatagttct gtatctcctt
    10441 ctaatggtga ataatcatac gtatcgccat catttgctga atcgatcaaa tgaacgaaat
    10501 caagatattg tctatctcca actatcaaat tcaattgccc atctttaaaa gaaagtttat
    10561 atttattgtt acttatctca gttttgtcta tctcgattgc tgtttctaat ttttcattat
    10621 ttttctcaaa ttcaacaaca gtgtatccta gtgctggcac atttacattt atttttatgt
    10681 caagttcata ataagctggt tcatcaaaat attcgaaacc tgtagcaatc atccgataag
    10741 catttttccg ttctggaaaa taaacttctt tttcaatgat attataggga ctacctttga
    10801 agtgtatttt ctttgaatca gctactacat gcacgatttt cgatccacta taattctcag
    10861 tcgttgtgtt gaaaacaagg acttcattcg attttagctt taatgcatca gccatccgtt
    10921 ttacaataag gttttcgatt ccgtcagcta tctcattagc ttctttgatt cgatggaaaa
    10981 tatcttccgc aacattatct gaaacacagc caccaatact atcatgagcc tggttttgca
    11041 aaatcttttt ccataaccga cgaagtaggc ctttactgat atggatacca tttctttctg
    11101 caatcagcat gagcggctca actcgtttta gaattttttg ttccaattta aaattttcta
    11161 ttttcatttt tgttcttact gagccaatag aacgatgaac acgagagtaa accggctgac
    11221 gaagttcacc tttatattca ggtaattctt ttttgtttct gataacttct acataagaag
    11281 gataatctga aataatattg tggaatttac tgtatttatt caacttctct acgacttgat
    11341 caaaatttaa aatcattgac ttttgatcta cacccgaagg tactaaaata tctttgtttt
    11401 ttccttgcat atgaagcatt tcaactgctt catccaactt ttcttttgca aattcaggaa
    11461 tatctttaat tgcgtctaag gtcatcattc cagtcatata accaaatgga aaattcatgg
    11521 cataaacaaa tttatctccc aatcctttcc agataaaata aggagatgga accatcgttt
    11581 caaagtttaa tcctcgccaa cacatgaaat tatcgatttc tacttgatgc aaaagtgtag
    11641 gcaaattcgc attgaaccca aacgtgtcag gcaaataccc taccatcatt ggatctccat
    11701 atttaaggat cgtatcactg attccaatca ttaagtttct taaaattgat tcagcatcca
    11761 ctaataaggc atctgtttgt gcataccacg gtcccacgaa tagttgtcct gttttcatca
    11821 gtcgttggat aaccttaagc ttttgcggat tgatctccaa atactcatcg acgattgaag
    11881 tttgtccatc taaacaaaag tttactttag ggttcctttc caattcatct agaacttcag
    11941 taaagacctg atcagacaat acgatggcat cttcatttgt aaaataccat tctctgtccc
    12001 agtgcgtgtg ttgaattata tgtgcattta ccatatcaga ttctcctact ttccttcttc
    12061 tactgaattt acttgttgtt cttttttata cttgatggat tttagtgcat tgaccattaa
    12121 cgctgtaacc actcccccaa taacaattga tactaaatat aaaggtatat tcgtaacaaa
    12181 aggaattgcc ataaaggttg aaagcattgt ttcttgagta acaccaaaaa atccatttag
    12241 accacatgct actgcagtgc ccaccacgat gcttggtatt actcgtaaag gatctttcac
    12301 ggcaaacggg atcgtccctt cagtaatccc cacaaggccc ataatcatag ctgtctttgc
    12361 attatctcgt tccgattcat cataaagttt tttgtttaaa attgttgcta aaaacattcc
    12421 aaatggcgga atagcaattc ctaataacat ggctgtacta ggtccaaagc ttccgcttga
    12481 aaaagaagca accccaaaag catatgcgat tttattgata ggtcctccca tatcaaacgc
    12541 catcattcct cctaaaatag ccgacatcaa agcagcattt gctcctgaca tgccttctag
    12601 ccaattagtc agtcctgttg tcattgcttg aataggcgta gctagtacat accataatat
    12661 gattccaaca actaatgtag taacaaaagg aatcagcata attgatttta gactaataac
    12721 ttgcatcggt acttttactt ttttcaatgc attagctaaa tagccacaag ctaatccagc
    12781 aaccattcct ccaataaatc ccataccaag attttgacac atcatcccag tgataaaagc
    12841 tggtgctaga ccgggacggt cagcaattga atatgcgata taggctgaaa tcatcggtac
    12901 gataaactgt aatcccgtct gaccaatcat tcgcaggact ccccagaatg tatccgtctg
    12961 gtcccaaaca cctgtgccac caaaaatgac tgcaatagaa gttagtaacc ctgctgctac
    13021 gacaagcggg atcatatagg aaaccccact ttggaaatga tttagaattt ctcttccttg
    13081 tttcttgaat tccattagta actcccccac gatatatttt ttatttgtta attaatgcaa
    13141 ttgcttcatc aatcgtctct tcaccatctt ttactacctc tgaggtccct actgttaaaa
    13201 tcggtttacc gtcaaagcga cctcgttctg aaacatttgt atctactgca aatatgacaa
    13261 catctgcctc ggaaacatcc gaaattgaaa gtttgttttc tatccccata gccccctgtg
    13321 tttctacttt aatatctaac cctttcttct tcgcaaactt ttctaaatta gcttttgcca
    13381 tataagtatg ggcaatccct gcgatacatg ctgttactgc tactattttt gtcatatgaa
    13441 attcctcctt ttaaaatgat agagctttta ccaattcttt tttatttctc gaatctttta
    13501 ttcttttaac aaactcgtca tccatcaatc ttccagataa ttctgctaaa atacgtaaat
    13561 gattagtacc tttatcagtg tcttttattg ctaacaaaaa gacgagatca actggttgat
    13621 catctaatgc gccccattca acagaccgct ttaacttggc tacagccact gttgattcaa
    13681 ttactgctag gcttttgcca tgaggaatgg cgatattttt cccaattcct gtggtcatat
    13741 ggttttctct gtcaaatacg tctttttgaa attgtttgtg gtcagacaca aacccattag
    13801 tctctaacaa tttcgataat ttttcgacta cacttttttt atcagatgca tttaaatcta
    13861 tcactatatt atttatattg attttttcac tgatcattta taagccttct ttcaatatat
    13921 aaactaattt ttcaaacgac gaactcgttt ttaaatttgc taataactca tgattcgaag
    13981 cgatttcatt gaatgtttca taaattttat tcaattcagg ttttactttt tcattaagtg
    14041 caaaaaagaa aacagaatga acaagtgtcc catcccaatc aattccttct ggattaacgt
    14101 agacacagat tactggcttt tttataaaat ctacttcgat atgcggcata gcaaaatcat
    14161 tcatcgctgt tgaagatagt ttttctcttt tgattgctcc ctctagtatc ccttctttcg
    14221 cgtaattttc ttttaccaat tttttggtaa ttatatttaa aacgttcact tgattatcct
    14281 ttatttggtg ggtaaaaaag caaaatcgtt tatctaataa atttagaaaa gcatcttctg
    14341 tttgttcatt taactgtaga tactgtttta cttttctgac atctgcccct aacatcaaag
    14401 gactaacaac aataacagga aaatttgtat tttcaatcgg aatcgtgctt ataacaagtt
    14461 catcagtgac ttttctatct tgtagttcat tgatgcttag tacatcttct atctcaacca
    14521 tatctgcaaa accgttattt attctttgct ctaataattt tgatgttcca tatccgctac
    14581 tacaaactaa tatcactttc gttttgtcag gtttgtaacg atccaaaagt gattgaatat
    14641 ggagtgttac gtaagcgatt tcatcttcat taaaatgtat acagaatctc tcttcgattt
    14701 cctctaataa atctacactt atcataaatg accttttaaa agaatatttg attttgtccg
    14761 tataagggtt atagatattt actcccaatt tgagtctttt aactgctgca ttgagatgta
    14821 atactagatt attgatcaat tcttcatcag gataaaattc atttaattta attcttacta
    14881 actcttgtag ctcaacaccc atatctaaat aggaaagctt cgtttgatct aagtcgtgat
    14941 tcatagcatt tttttcaatc gaagtaatgt gaatgtcaac ataagcttgt tcatctattg
    15001 gcacaataat tgaaaatgct ttttcaatca ttttgattaa aataagtgta ttctttgaga
    15061 tttcttcttc agaattatta ctacgtgata agaaattgct tgtttcaatc ctttctatcg
    15121 caatagcaag gtggatagct aaagtttgaa attcataatc tgtaaatttc aattgtgtct
    15181 tattcatgta tcgattaagc aattcggcta cttttttcaa tatgtcctta tttaagaaat
    15241 tattcgcttc accaatgaaa ttgatattca agtctaagag atctgtttta ctgttagatg
    15301 ctgtaaagcc accccaataa tagttcacaa cttctgacat caaccttctt ttactttttt
    15361 catcagcttt gatgcgcacg ccatttcgat ctgaaactaa ggacatccca tgcttgttta
    15421 gtctttttct aacatctttc attgtgcttt ctaccgtcga cctgctcacg tataattcgt
    15481 cagcaagatt ttgaatcttt agagaatcat tagtattcaa taatttagca aacacataag
    15541 ttagacggtc ctccggagag tctgcgatat tgattttatg actggataat aaatttcgaa
    15601 gtgtttcttt ttttccaata agcgaaacgc cttttttagg ttgaaccaac atttgtacat
    15661 ctggcatttt ttggattatt tctttcaaaa ttaaaatatc tttgctaatt gttttcgtag
    15721 atacatataa ttgttctgat aattctttgt aagttgtgtg ctcattttca atcaaaaagt
    15781 atagtatttt catttgccgt tcgctaaatt cgatgcccat tatcttcacc tcctgaatac
    15841 agcttaagtg tacaattatt atctctattt tcatacagaa gaaagttctt gattaatgta
    15901 gaagtttcta atattcttga ttatttcaaa tcaataaa
//
LOCUS       DAZKOU010000064        15794 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000064 DAZKOU010000000
VERSION     DAZKOU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.065"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1574
                     /locus_tag="ACG827_001993"
     CDS             147..1574
                     /locus_tag="ACG827_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484123.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1666..2031)
                     /locus_tag="ACG827_001994"
     CDS             complement(1666..2031)
                     /locus_tag="ACG827_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0484124.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2028..2393)
                     /locus_tag="ACG827_001995"
     CDS             complement(2028..2393)
                     /locus_tag="ACG827_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0484125.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2585..2833)
                     /locus_tag="ACG827_001996"
     CDS             complement(2585..2833)
                     /locus_tag="ACG827_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484126.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2832..2960
                     /locus_tag="ACG827_001997"
     CDS             2832..2960
                     /locus_tag="ACG827_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0484127.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3017..4711
                     /locus_tag="ACG827_001998"
     CDS             3017..4711
                     /locus_tag="ACG827_001998"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0484128.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4778..4984
                     /locus_tag="ACG827_001999"
     CDS             4778..4984
                     /locus_tag="ACG827_001999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484129.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5362..6495
                     /locus_tag="ACG827_002000"
     CDS             5362..6495
                     /locus_tag="ACG827_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0484130.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6526..6846
                     /locus_tag="ACG827_002001"
     CDS             6526..6846
                     /locus_tag="ACG827_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0484131.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6875..7339)
                     /locus_tag="ACG827_002002"
     CDS             complement(6875..7339)
                     /locus_tag="ACG827_002002"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0484132.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7471..8724)
                     /locus_tag="ACG827_002003"
     CDS             complement(7471..8724)
                     /locus_tag="ACG827_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0484133.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8753..9070)
                     /locus_tag="ACG827_002004"
     CDS             complement(8753..9070)
                     /locus_tag="ACG827_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0484134.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9096..9416)
                     /locus_tag="ACG827_002005"
     CDS             complement(9096..9416)
                     /locus_tag="ACG827_002005"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0484135.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9880..11295)
                     /locus_tag="ACG827_002006"
     CDS             complement(9880..11295)
                     /locus_tag="ACG827_002006"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0484136.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11312..12628)
                     /locus_tag="ACG827_002007"
     CDS             complement(11312..12628)
                     /locus_tag="ACG827_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0484137.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12778..13476)
                     /locus_tag="ACG827_002008"
     CDS             complement(12778..13476)
                     /locus_tag="ACG827_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0484138.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13765..14754)
                     /locus_tag="ACG827_002009"
     CDS             complement(13765..14754)
                     /locus_tag="ACG827_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0484139.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14834..15667)
                     /gene="msrA"
                     /locus_tag="ACG827_002010"
     CDS             complement(14834..15667)
                     /gene="msrA"
                     /locus_tag="ACG827_002010"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0484140.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
BASE COUNT         4916 a         3198 c         2698 g         4982 t
ORIGIN      
        1 tatctgtttt ccaaatttta taacataaaa aacctttttt aacggcattt atatgctttt
       61 tcagaaatta actgatgatt tcaaaaagaa aacatgtcat aatatttccc atattgtgca
      121 aaaaaataca aggcggaggc tctactatga ttgaagaata tattgagaaa aatattttaa
      181 gacagctgtt tttatgtggg caattctatg taaataaaga agtcaatcta gaaaagctct
      241 ccaatctcct gcatgtttgc aaaaccactt tactcaatga tatcaataac ataaaaaaag
      301 aatttgaaga acagatcgct tatacacatc gggaaaagga ccgttacact ttatattttt
      361 cagaacatat acctcgatgc aagatcatgc agcaattgtc tcaaaattcg ttgtttttaa
      421 aaacttgtct gctttatttg gaagaagatg aacctgatta tcttcaatta actgaatgtg
      481 aattcatctc tgtcagcaaa gcttattcat taaaaaaaca agtattagct tattttaatg
      541 actgcgggat cgaaatcgac cgctactccc ctcgattcac tgaaatggaa agacgtctgc
      601 tgcttcttaa tgtgtcgtat cgtttaggcg gttttaattc ttgggagcta cctgaatcgt
      661 tttttgaacg tgccgatcgt ttcattgaat ccgtaacgga aaatagtggc agattttatg
      721 ataaagaaaa taaagaaatc ttatcgattg gttttgctat cagtttccta cgccagcaag
      781 tgtgtgctgt gacaatagat tcaaaattta tcgaagaaat caaaaaacgt cctatttaca
      841 attatgtcga atcagcatgg gaaaacactg atttccaaac ctattataaa aaagaagaat
      901 tcgcctttat tttgacacta ttcaatcttt gtaattatgg tttccattct tatcagttga
      961 ttgcggaaga ctttcaacag ctacaccaag tgtttattga caatactccc gaaataaaag
     1021 aactagttgc tacctttgaa tctcacttca atcaagaatt attcggaaac caaccgttcg
     1081 aacgtgcgct gattcatctg atgcgttcgg cttgggataa ctatcaatta ttcatgccag
     1141 aaaaatttta tcttttaaat gaggaacaga caaatttata taaagaagtc cagacaattt
     1201 tttccagctg gtccagccag ttaccttatg atctacggtt gaatcctaat tgcatgcggg
     1261 cttttgtgat cgaattgagc ggaattctcc gtctgacaaa agaacatctg actatctata
     1321 tcgtcacaaa ttcggatgtt cattatctga tttatcggga agccttggaa gctgtaacta
     1381 ctttcgattt tcaagttgca ccaacaattt attcaagtat atctgatata aagaagtatg
     1441 cgcagcagtc ctcaaaccgg gtattatgtg aacgtacatt atatacacca gatgcggtcc
     1501 aatatgaaaa tattattcct atttcgatca atacgatcga tcgagctatc atttcagctg
     1561 tgcaaaacaa ataaggggct gtgacaaaaa tcatgtcaca gcctcttatt tgttgaagat
     1621 gcccatttct gtaacaatct cttgatacac gtcaggctca caacctcact tattttggac
     1681 atctacgggt ccgatctctt tatttaaaaa ataagaaatg atggttcgga taactaccaa
     1741 cgccgctagt tggaaaatat cctgcaaggt aggtttgata atcgattcga tgatcccagc
     1801 tgaaacaaga atttccaaac ttagaagtat atagcctccc aacatctttt taatagcatt
     1861 gttctcgctg actcggtcgc tttgcacttt tgtatggaca aataaatatt tcaaaaaatc
     1921 cttgattgat agacaaacac cccaaaccaa taaacagata gcaaagatat caagtcccag
     1981 tacaagtttt tcgaaatatg gaagaagaaa ctgcatcaac tctgtcgtca tcctacctct
     2041 cctcttcttt gtttttcttt gtcttctatt tcctgattca agaaataaga aatgactgtt
     2101 cgaatgataa taataagtgc aagcttcaaa atatctgtcc aagtcggctt gataactgac
     2161 tcgatgatat cagcaacgac tagtacttcc aagcttaaca aaatataact tgctagcagt
     2221 tttcttgtat ctgcattacg agtattacgt tctgcatagt ttttcttgac atgagaccaa
     2281 tcaaaaagtt tgaatagaac aagaacgatc ccgacaatta agataccgat agaacaaata
     2341 tttaaaatag caatgatcca ttctagaaat tcaattaaat ttgagcgaaa catatacgct
     2401 acctactttc ttttaatttg ttcctattct tttattgtaa aagaaagtta ccttgttcgt
     2461 ctatttaaaa gttgttctaa tgataacggt tcgcttaaat ttttcttttt cactctccca
     2521 tttttctccc tccatccata aatcgtggaa gtatccaaaa caacgtagca agttaaaata
     2581 aggcttagtc cgtcatcagc ttttcaaaga agcactgtct cggtgctttt tttgtaccca
     2641 aatttctaaa aatagaacca tacacctcct caaatctttt cttcttacct accctatttt
     2701 tatttacagg aaaatcagac acttcttctt ttttgtcaaa atttcctttt tcttctcgtc
     2761 ctatcatttg gcattctaat aaaaacgaac tatccttgga tatagaaaga gaaaacaagg
     2821 aggcgttttt catgagtaaa aaattaatca aagtcggcat cggtttaggt ttgcttgccc
     2881 ttggcgctgc atatctagga aaaaagacag gactattcga agatgacagt catttatatg
     2941 atgaattcga atcaatctag catcctattt agttgaatca ctacggacat acttggaaga
     3001 cattggagga atcacaatga gaaaaagtaa agcaataatg attggtgccg gtattgccaa
     3061 tatggcagcg gctgtttatt tgattcaaga aggcaaatgg aaaggggatc agatcacctt
     3121 ttattcattg gatgatcacg gttcaaatga tggtgcaccg accgacactg tagtggatga
     3181 atattggaat aaaaaccatc cattagaaaa ccaaaaaggc tatattgctc gaggcggtcg
     3241 catgcttaat taccgtacct atgtagattt gatggatctg ttaagtcgaa taccttctgt
     3301 tactgagcca gggatgactg ctgaagaaga tacgagagat tttgatgcaa aacaccgaac
     3361 ctttgacaaa gcacgtttga tggaaggtgg caaaggaatc attgatggcg gaaaactggg
     3421 attcaataat aaagaccgta ttcttctgac taaactgatt gctatgcctg attcagaaga
     3481 agaaaaacta gacaatatca ctatcgaaga atatttcaaa gatgatccac acttttttga
     3541 aacaaacttc tggtttatgt gggaaacaac atttgctttc agaacacgta gttctgctca
     3601 agaattgcgt cgctatatgc atcaaatgat ttatgaattc actcaaatcg aacatctagt
     3661 gggcgtcaat cgaacacgtt acaatcaata cgaaagtatt atgcttcctt tgatcgagta
     3721 cttgaaagaa caaggatgca aaatcatttt gaaccgccgt gtcactgact gggaattcaa
     3781 agaaacaccg atgcaagatg aaatcactgt gactggtttg aaaattacca atacactgac
     3841 tcaagaagaa gaatacgttg tggtcgatga agatacagca gttattttca ctaatgggtc
     3901 cattacagat tcagctactt taggagatta cgaaacacca gctgtagaaa atatggatta
     3961 tggtgcagct tctagtctct ggaaaaaagc cagtgaacgt ttctacaact tgggaaatcc
     4021 tgacaaattc tttgctgatc gagatgcaag cgaatgggtc agctttaccc tgacgacgaa
     4081 aaaccatttg ttgttgaatg aaatcacccg tatcacgact caagaaccag gaaatgcctt
     4141 gaattcattt atttcaacca caccgatcac gccgttaggc caaaaagatg tcaatatgtc
     4201 tattgtcgtg catcatcaac cgcattttac gtctcagaaa ccaaatgaaa cagttatttg
     4261 gggttatttc ctttatccaa gacgacgtgg cgaatttgtg gataaacagt atattaaaat
     4321 gaatggaaaa gaaatgctag aagaactgat tggacaactt tcaaaagtag atcctggacc
     4381 agtgaatatt cgtgaaaaag aaacagaaat tttcgacagc gtaataaata atatccctgt
     4441 ctatatgcct tatgcttccg cgttgttcaa taatcgtgcc aaaagcgatc gaccaaaagt
     4501 cattcctaaa cattcgacca atctggcctt tacaggagaa tttgtcgagc agccttatca
     4561 gatgatcttt actgaacaaa gtgctgtacg ttctggagaa atcgctgctt tccattttgc
     4621 gggtattcct atgtcccgtc ttgtcaaaac accgcgttac gacaaagata ttcctactct
     4681 agctcgtgcc gcaaaaaaaa tgtttgaata atcaagaggt tgtgaaaaag ctgccctgca
     4741 tcaagtaata agaacaacca ggctaaaaca gcttcttatg tttttgattt ggttggactt
     4801 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aagctgccct
     4861 gcatcaagta ataagagcag ccaagctaaa acagcttctt atgtttttga cttggttaga
     4921 cttattaccg cagatgcaag cttttgaaca ccgtttacca agaggttatg aaaaaggcgt
     4981 ttagtttcga gtatgaagaa aaattttgtg aaaatggttc ctcacgtttt tgcataaatt
     5041 tgacttaata ccgagaaact gatcccttct gtcataacct ctttttattt atgtctgttt
     5101 ctttttgaaa acaactattt tttcaacgtt tactctcttg ccaatccaac atcatctaac
     5161 ggatacaact atttcaatga ttaccattac actaaaaaat cggaaacaca cctctccgaa
     5221 cacaaataca taagactata gaatgtttca tttaagaaaa aaagcgtttc attatcaaaa
     5281 aggtaagatc tcctttaaaa tgaaattatc tgtagtacta ataatgcaga caattttatg
     5341 aatctgaaag gagcatctcc aatgaaagca gtcacttggc aaggaaaaca gaaaatggaa
     5401 atcagaaacg tagatgatcc aaaaatatta gaaccaacag atgcaatcat ccgtatcaca
     5461 gcaacagcca tttgcggctc cgatctgcat ctttaccatc atggtgaagc agttttggaa
     5521 aaagggtatg ttgtaggaca cgaaccaatg ggaatcgttg aagaagtggg atctcaagtt
     5581 caaacattga aaaaagggca acgtgtggtt atccctttta atattagttg tggtcattgt
     5641 catttctgcc tgaatcacat ggaaagccaa tgtgatgaat caaatccaga tcagttatat
     5701 ggcgggcttt tcggatttgg caagttgaac ggtaatcatt ggggtggtca agcggaatat
     5761 ctaagagttc cttatgcaga ctctacttca tttgttgtac cagataatga tcttcctgat
     5821 gaaaaagttt tatttctatc agatattttg cccacagctt attggagcgt tgaaaacgca
     5881 ggagtaaaaa aaggcgatac agttgtgatt ctaggttctg gtccagtcgg tctattcgct
     5941 caaaaattcg ctgttatggc tggagcagag cgcgtaatcg cagtagatcc agtacagcac
     6001 cgtctagata aagcggctaa ttacaatggg gttgaaactt atttcttaga agacacggca
     6061 caagctggaa cagaattgta tgaactggct aaaggtggcg cagatgtcat tatcgattgt
     6121 gtaggaatgg acggattaga gcctgtcaaa gaaaaagcga aaaatttagt aagcttacaa
     6181 agcggcacaa tctctccatt acagatggca tcgcaagctg tcaaaaaatt tggaacagtc
     6241 caagtaactg gtgtttatat gactccagct tcttcttacc cgctgcaaga attttttatg
     6301 cgggatatcg ctgtcaagca tggacaggca ccaattatcc atctaatgcc aaaaatttac
     6361 gcaatgattg cagaaggact attcgatcct acacaaatca ttactcattc gctgccacta
     6421 tcagaagcag cacaagcata tgagatcttt gacaaaaaag aagataaaaa tataaaagtt
     6481 gttttgaaac cttgaattca gtaaaaaata caggtgattt tctttatgcc caaaatcaaa
     6541 cgaaataccc atgagatcga aataggaaaa gaagaagata tcgaaatacg aggacgtcgc
     6601 ttccgcttct atttccaaaa tcgttacaca ttgatctcac tggctgtcga tttattaacc
     6661 gggattttct atatttttgg aagccttgct tccttaacta aaatccccga tcgatatggc
     6721 acctattttt atttggctgg tgctattttc ttgacgattc gtcctattct cagaatcgta
     6781 aggaacattt ttatttacga cgaaaaaaaa gcaatgaaga gaacaagcaa aaaaaaagag
     6841 gcatgagaat ttttcaaatc tcatgcctct tttttcaccc tatcgtatca ctttgataca
     6901 gacgagcggt cgtcccactt tcttccttga ccattgtcaa aaatgtccac tcgtatcgtt
     6961 cataaaacgt ggtatgatct gtaattaagt aaagtttttc gtacccaagt tgatgcattt
     7021 cttttctgat ttccgctaat atccgcttgg ctatcccctg cttacgcttt tccggttcaa
     7081 caaacagggc acaaagattt ggtgtcagat cagggcggtt atggaagtcg ttttcaatga
     7141 cgcccgctcc tccgacgatg gtttcttttt catctaagac cacataccat tgaggaatac
     7201 tttccttatt agcgattcca gtacgaatac tttcctcata cacaacagca ggaactcccc
     7261 attttgatga aaaccaattc gcgctttgtt ttactaattc tgggtgttct cttaatttga
     7321 tccagttata tgtaaccaat gtttaaacct ccgactaaaa taaaacgttt acaaaaatta
     7381 tacaagtctt tatgaaaatt agcaatgaca acaatagtga tgtaaaaaaa aaaacggaaa
     7441 acaatcactt gttctccgtt ttttgattaa tcaagcatct aaagcttctt ttcttctttc
     7501 ttggatcgta gctaccttga cgaacggtag atagatcaaa atagaaagaa ccaaattgac
     7561 agcggctagt acaccacctg caatactttt tgttgccaat acaccgccaa taattggtgg
     7621 agttacccat ggaggcataa cggtagctgc tggcactaaa cctgtagaag tcgcaaagta
     7681 agcaacacta actaagacca ttggtgtcaa aataaatgga atgaacatga tcgggttcaa
     7741 cacgataggt aacccaaaca tcgttggttc attaatattg aagacacctg gcgcaatcga
     7801 caagtttgtt acaaccatat atggtttgtt tttacgtcct actaaataaa tagctaacag
     7861 taaacctaaa gttgcacctg ttcctcctaa gttgacaaag ctgtcaaaga aaggtttgtt
     7921 cacgataaac ggtgcgctat gtccagctgc gattgcatta acgtttgctt caatggctgg
     7981 cgcattgatt gtttgcatca aaggatcgac catattcgct ccgtgtaaac caaagaacca
     8041 taagaatggt gtgataaatg caagcagtaa ggcagatgga taagagtttg caagtcccat
     8101 aaatggttct tggaccactg tatagaaaga acctacgata tcatcaacac cgaatgcagc
     8161 aaaaattgca gcaactaaag caaacgcaga gatagtgatc attgctggta gtaatgaagc
     8221 aaatgctttt gcaactgccg gtggcacacc atcaggcatt ttgatgatca atttttcatt
     8281 tccgcttaaa cgtacaaaga tttctgttga aataatccca acaataatag caataaacaa
     8341 accgtttgag ctcataccta atgctccgcc taaagcgaag aaagagccta aagaaatcac
     8401 acctgcggaa acaccgtctt ttcggtagcc gttagctaaa tgataagcaa gcaagaaagc
     8461 aatcaagaca gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca
     8521 tgattcacca ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt
     8581 gatcattact gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg
     8641 aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc
     8701 cataaatgca acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt
     8761 tcaatcgacg atacagatca atgatctctt ttgctaaatc tgtaaaagct atagatgtca
     8821 tcagatgatc ttgactgtgg accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt
     8881 cttgtgtcag catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt
     8941 tgattttttc atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg
     9001 catcgctttt tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt
     9061 gatcttccat ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct
     9121 tggtcgagta ctttttcacc attcatcatg ccatagtcag ccatattgat tacatctaaa
     9181 ggaatgcctt taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt
     9241 cctaaaagta aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat
     9301 acagcaaaga tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact
     9361 aggagacttg tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta
     9421 aacacgttcc tttctatact tacacttgta tataagcaag tagcgtgcca actttaaaag
     9481 cgctttaaaa agttgatatc atcacattta aggtctatac caagttgcat acacactaaa
     9541 aaagaaagcg cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc
     9601 acaaaaaaag ttgtgacaca aaacttgtca caactttttt caaataaacg gtattcaaaa
     9661 gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca taagatgttg tttttacttt
     9721 gctattctta ttacttgatg caggacagct ttttcacaac ctctcaataa acggtgttca
     9781 atcagctgac cctgggtatt agctcaaaac gctggattca caacctcttt tatttcaaat
     9841 aaccagtaaa tacaatgaac atctacaaaa aacttctatt tatttataga tttcgtcagg
     9901 caaatccaat tctctcttat caatcaaagt ctgataccac gaagcagatt ttttcaatgt
     9961 tctgcttcgt tcatcgttca attcgattcg aactaatcca tatcgattct taaatgcatt
    10021 cattggtgac acacagtcag tgaatgccca taacatatag ccttcgcaat ttgcgccttc
    10081 ttctgttgct ttgatcaagt aatgcaaatg ctgtccgata aattcgatac ggtaatcatc
    10141 ttgcacgatt cctttctcat ccatatactg ttcttcatat tctcttccca taccgttttc
    10201 tgttatcagc caagggatat tatgataatt ttcttttaga tacatggcta tatcatacat
    10261 gattttcgga tagatttccc atcccctgga attattcatt ttcttcccag gtaaatcaaa
    10321 gacttcataa tatcgttcag gatggaaagg tgtctcttca ttccattgat aacgaggggc
    10381 gcgaacgcgt ttaggataat attgattgat tccaaggaaa tctactgtat gatgctgaat
    10441 cgtttctaaa tcctctgttg caggatcgaa ataaatcgat tgtttttgac acaactccag
    10501 gaactcttct gtgtagtatc cttttaccat cggatcaaaa aatactcgat tataaaacaa
    10561 atcatacata taagcagctt tttggtcttc tggagaattg gatcgagcat agaccatttc
    10621 aggatttaaa atacagccaa tccttccacc ataatttccg ctgcggtaaa tctctactgc
    10681 tttagccgtt gctaaaactt tatgataatt ccacatcatc cacttttttg tgttttgttc
    10741 atgcggccag cgaacagcat ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa
    10801 tgtaaaccag ttttttaccc tatcaccgta acgatcaaag gcaatttgag catacttagc
    10861 ataaaaatca actacttttc ttgaagacca gccatcatat ttttccaaaa gaaaagctgg
    10921 gacttcataa tgttccaaac aaatcatcgg ttctacgtct gcttcgatca aagagtcgat
    10981 catttcactt atatgctgag catattcttc atctactata cagttctcat aatcagtaaa
    11041 aaaacgagcc caattgatag aagtccgata atgtgtcaga tggatggatt tcatcagatc
    11101 cacatcctgc tggtattgtt ccataaaatt agttgctacg gctgggccat acccattatg
    11161 ccaaacaaaa tgttcatttt tataccagct atctataaac gaatcctgtc cttctttttt
    11221 tcctttccaa ccttccgttt gccaagcaga agatgcagca ccaagaataa aattttttgg
    11281 aatagtaatt ttcatgattt cctcctgttt tttagatccc atcattttcc acacgatttg
    11341 ctacttttac aaatggcaga tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa
    11401 taactgcacc gatatttcct gctgttgcca gataagcatt gacgatcggc ggcattggcc
    11461 aagggaccat tacaactgct ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg
    11521 ttccagtgat aagaggcgtt aagataaacg gaatagctaa aattggattc agcatgatcg
    11581 gcataccaaa aataactggt tcattgatgt tgaagatccc aggagcaagg gataacttgg
    11641 tgatttgttt catatcttcc cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg
    11701 tgactccaga cccgcccata ctcatataca tatcccaaaa tggcatagta ataatgttcg
    11761 gtacttcttt tccagcttca aaagcttccg tatttacagc aattgccgct aaaagcaacg
    11821 gttcccgtat cggcttcacc atttgatttc catggatacc aatcacccaa aagacttgcg
    11881 caataaacat caatagcaaa atacccggta acccttgtac gacaccttct aatgggcgtt
    11941 gtacgacatt gtaaatgata tcataaaggt acattcctgt aagttctttg atgataaatc
    12001 ccgcagccgc gatacccgat acagtcagaa tcgtcgggaa taaagcagaa aaactagcag
    12061 aaacattcgt tggcacggta tcaggcattc tcagctgtag tttcttctgt ttcctcaacc
    12121 agcaataaag ttcaatagaa aggatggaga ccaacattcc caaaaataac cctttcgtat
    12181 ctgtatattg tttagccaat acgttttcta ctataacagt ctgattttca ttcactaatt
    12241 cataaacagt tggattaaca gataaataag acatcactgc gatgatacca gggaatacac
    12301 catccacttt attcagcttg gctaattcga tccctatcag aaaaacagca tagatggtca
    12361 agaaacttag tgtgacatag ctgatggcag atgcgatagg ctttaattct tctaaaaaac
    12421 gcaagaaact tatctgtgct aagccatttt ccgagtcaaa gaccattgct gaaaacaatg
    12481 tgccaaaagc acccgtaata attaccggaa tcaatgcagc aaatgcgttt tttatcacca
    12541 tgatatacct taaattattg attctcgttg ccacgttccc taaaacattg gctaatttat
    12601 ccataaaact cattccagat cccctcattc ctgcttcttc tccttgacgt catctctttt
    12661 tgtctattag tcagcgctta catttcgatg aacagaaact atttactcct cttttttacc
    12721 aaatataaaa caaaaattca tctgatgtat ttatcataaa atacatcaga tgaactgtca
    12781 attagtttct ttttctccta ttattttctg taattctctc cgatttattg ccatatggac
    12841 agtcattgca tcaaacgcag caagtgcatt ccgcctttcg atggcctgaa cgatctctgt
    12901 atgaatatcg atcgtctcga ttttcagatg ctggatatca taatttttat taaacaaagc
    12961 aattgattca ttaatgattg ggataatatg cagcaaggct acgtttccgc tagcttctgc
    13021 aatcatcgta tgaaaatcga tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga
    13081 agtttcaatc ttttgcgtta attttttcat ttggacgatt tgattcttcg ttgcatattc
    13141 cgctgccaaa cctgccatct taggctccaa taaatatctc agttcaaata agtctcgtat
    13201 catccgatcc tgatcttcaa tcaaagaaaa accgagtggg tcattggcaa tccctcgctt
    13261 atcactgaca aatgtccccg ctccttgccg aacttcaagg atattcctgg aagctagagc
    13321 ccgcacagct tctcgcaccg tgcttcgccc cacctctaaa taatcggaca attcatattc
    13381 attcggcagt ttatctccta cttgcaactg catttccttt aaatatctaa ggatcttgtc
    13441 agtagttact tctactaggg ttttattttc ttccaaacca catacctcct tttcatctga
    13501 tgtatttttt cgtaattatt ataacacgtt tgaatcatct gatgattttt tgtatacaaa
    13561 attgattaat ccgttcatag acatacaaac gaagaagata tttaccataa aaaagactag
    13621 gataaaattc actcctagcc ttcgatcatt tttattttga acgacaaaaa atatgatgtt
    13681 caataacaga cgatcttcat tcgattcttg atgtcatctt ttgttttttt taatatatgg
    13741 ataaaaagtt tgattacatc gtttctattc tagaatttgg tcgattctca attcttcttc
    13801 tggtgagaaa tctaaattat ccatcattcg aacactttct tgtaactgac ttaaccgact
    13861 tgctccgacc agtacacttg taacagattt ttgtcgcaaa ttccatgcaa gggccatttg
    13921 cgctaatgat tgtcctcgtg atacggcgat tgtttgcagt gctttgattt tttccagagt
    13981 tgatccaact tgttcttctg aaaggaatgg tatttcctta cgatgtgctc tagaatcttc
    14041 aggaatacca tgtagataac gatttgtcag caacccttga gcaagcggac tgaacgtgat
    14101 tgcccctagc ccttcttctt ctaatactgg agatagatcg tcttctagta gtgcacgaga
    14161 aaacatattg tagcgcatct gatgaataat gaacggtgct tcctttcttt ttaaaatttc
    14221 agtcattttt tttgtatctt ctccattata gttggatata ccaatatata atgcttttcc
    14281 ttgtcttaca agttgcatca aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg
    14341 gcgatgatga tagaagatgt caacataatc tagccccatt ctttggagac tttggtcaca
    14401 acttgaaatg atggatttac gagagcccca ttccccatat ggcccttccc acatatgata
    14461 acctgctttg ctagaaataa tcagttcatc acggtaggct tgaaaatctt ctctaaaaat
    14521 acgcccaaag tttttctctg cagctccagc agggcctcca taattattcg caaggtcgaa
    14581 atgggtaatt cccatgtcga atgcgcctct taaaatctct ctttgattat ggataggatc
    14641 ataatcacca aaattttgcc ataatccgag agataaaatc ggtaatttca aacctgaatg
    14701 accggattta cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc
    14761 tcctcctcat caaatctttt taaaacgctt tcttttttct atttttagtt tactacattt
    14821 taaagaaaag gagttaatca aaatgatttt ttcgtatatt tttttgccat ttgtttttta
    14881 ctcgttgata gatcagaaat tgattacgtg cttgctggat ttttttataa cgtttcggtt
    14941 gttttttgta aaattgctga tgatagtttt ctgctggcca aaacgtaccg gctggttcga
    15001 tttttgtgac gattggttgg tggaaagttc ctgaactgtc tagtttctgt tttgattgtt
    15061 ccgctaattc tttttgtcgt tcgacttgat aaaaaatcac tggacgatat tgcttccctc
    15121 tatcttgaaa ttgtccaaat gtatctgtgg gatcagtaat ttgccaatag attgtcagca
    15181 actcgctata acttatttct cttgtgtcat aaatgatctc cacagcttcc acatgacctg
    15241 tgtaaccacc acttacttga tcataatttg gtttttcaac atgtccgccc gtgtatcctg
    15301 acaaaacaga taggatccct tttttcgatt caaacggctc gaccatacac cagaaacatc
    15361 cccctgcaaa tactgcacgt tcttgatagt cagcatcttg catggcatgc ttggaaaagt
    15421 catatttttg ctccttatca aatccttccg taatcaataa ataaaagtcc attacatcag
    15481 gcgtcagatt cattcggata gctaatggac gtagtgttgc ttctagtttt gataactgtt
    15541 ctttaacgga tatattttct cttatgtcat tctttgtctg aatcaatact tttcgttccc
    15601 aatctcgtgt attcggattc aaaatcagat tataaagtgt ttgaatgaat atttgttcct
    15661 catccataga ttctcctcct ttccgaagaa gacaagctag tgactttagc tgtaatttat
    15721 cataaagtga ggaaataagc gattacttca tccccctaca gatttttcgt ataaaaaaga
    15781 gagaatcctt atcg
//
LOCUS       DAZKOU010000065        15743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000065 DAZKOU010000000
VERSION     DAZKOU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.066"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG827_002011"
     CDS             complement(24..722)
                     /locus_tag="ACG827_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0484141.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG827_002012"
     CDS             complement(715..2067)
                     /locus_tag="ACG827_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0484142.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG827_002013"
     CDS             complement(2290..3690)
                     /locus_tag="ACG827_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0484143.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG827_002014"
     CDS             3851..4588
                     /locus_tag="ACG827_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0484144.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG827_002015"
     CDS             4713..5192
                     /locus_tag="ACG827_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0484145.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG827_002016"
     CDS             5189..5956
                     /locus_tag="ACG827_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0484146.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG827_002017"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG827_002017"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0484147.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG827_002018"
     CDS             complement(6859..7461)
                     /locus_tag="ACG827_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0484148.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG827_002019"
     CDS             7567..7797
                     /locus_tag="ACG827_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484149.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG827_002020"
     CDS             7788..8036
                     /locus_tag="ACG827_002020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0484150.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG827_002021"
     CDS             8142..8726
                     /locus_tag="ACG827_002021"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0484151.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG827_002022"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG827_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0484152.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG827_002023"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG827_002023"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0484153.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG827_002024"
     CDS             10627..10806
                     /locus_tag="ACG827_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484154.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG827_002025"
     CDS             10828..11853
                     /locus_tag="ACG827_002025"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0484155.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACG827_002026"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACG827_002026"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0484156.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACG827_002027"
     CDS             13425..14372
                     /locus_tag="ACG827_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0484157.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACG827_002028"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACG827_002028"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0484158.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACG827_002029"
     CDS             complement(15274..15516)
                     /locus_tag="ACG827_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484159.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACG827_002030"
     tRNA            15637..15709
                     /locus_tag="ACG827_002030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5350 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tta
//
LOCUS       DAZKOU010000066        15662 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000066 DAZKOU010000000
VERSION     DAZKOU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.067"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG827_002031"
     CDS             <1..1087
                     /locus_tag="ACG827_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0484160.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACG827_002032"
     CDS             complement(1305..2447)
                     /locus_tag="ACG827_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0484161.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACG827_002033"
     CDS             complement(2452..2637)
                     /locus_tag="ACG827_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0484162.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="ACG827_002034"
     CDS             complement(2991..3236)
                     /locus_tag="ACG827_002034"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484163.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="ACG827_002035"
     CDS             complement(3230..3631)
                     /locus_tag="ACG827_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0484164.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="ACG827_002036"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="ACG827_002036"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0484165.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="ACG827_002037"
     CDS             complement(5649..7415)
                     /locus_tag="ACG827_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0484166.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="ACG827_002038"
     CDS             complement(7428..8222)
                     /locus_tag="ACG827_002038"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0484167.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="ACG827_002039"
     CDS             complement(8232..9608)
                     /locus_tag="ACG827_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0484168.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9728..10375)
                     /locus_tag="ACG827_002040"
     CDS             complement(9728..10375)
                     /locus_tag="ACG827_002040"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0484169.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="ACG827_002041"
     CDS             complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="ACG827_002041"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0484170.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="ACG827_002042"
     CDS             complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="ACG827_002042"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0484171.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13042..14673)
                     /locus_tag="ACG827_002043"
     CDS             complement(13042..14673)
                     /locus_tag="ACG827_002043"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0484172.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14977..15648
                     /locus_tag="ACG827_002044"
     CDS             14977..15648
                     /locus_tag="ACG827_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0484173.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         5075 a         3060 c         2112 g         5415 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
    10441 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
    10501 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
    10561 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
    10621 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
    10681 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
    10741 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
    10801 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
    10861 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
    10921 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
    10981 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
    11041 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
    11101 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
    11161 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
    11221 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
    11281 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
    11341 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
    11401 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
    11461 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
    11521 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
    11581 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
    11641 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
    11701 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
    11761 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
    11821 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
    11881 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
    11941 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
    12001 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
    12061 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
    12121 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
    12181 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
    12241 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
    12301 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
    12361 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
    12421 ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
    12481 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
    12541 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
    12601 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
    12661 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
    12721 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
    12781 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
    12841 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
    12901 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
    12961 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    13021 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    13081 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
    13141 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
    13201 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
    13261 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
    13321 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    13381 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    13441 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
    13501 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
    13561 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
    13621 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
    13681 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
    13741 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
    13801 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
    13861 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
    13921 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    13981 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
    14041 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
    14101 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
    14161 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
    14221 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
    14281 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
    14341 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
    14401 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
    14461 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
    14521 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
    14581 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
    14641 gatagaattt tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
    14701 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
    14761 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
    14821 ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt
    14881 tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa
    14941 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
    15001 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
    15061 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
    15121 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
    15181 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
    15241 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
    15301 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
    15361 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
    15421 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
    15481 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
    15541 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
    15601 caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact tttaactctt
    15661 tt
//
LOCUS       DAZKOU010000067        15255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000067 DAZKOU010000000
VERSION     DAZKOU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.068"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1021
                     /locus_tag="ACG827_002045"
     CDS             2..1021
                     /locus_tag="ACG827_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0484174.1"
                     /translation="MSIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLIT
                     GKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFF
                     TSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMI
                     ISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSV
                     FLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNIL
                     LFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTV
                     RRLKR"
     gene            1018..1785
                     /locus_tag="ACG827_002046"
     CDS             1018..1785
                     /locus_tag="ACG827_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0484175.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="ACG827_002047"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="ACG827_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0484176.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="ACG827_002048"
     CDS             3750..4070
                     /locus_tag="ACG827_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0484177.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="ACG827_002049"
     CDS             4070..4420
                     /locus_tag="ACG827_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0484178.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /gene="hprK"
                     /locus_tag="ACG827_002050"
     CDS             4587..5525
                     /gene="hprK"
                     /locus_tag="ACG827_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0484179.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /gene="lgt"
                     /locus_tag="ACG827_002051"
     CDS             5540..6370
                     /gene="lgt"
                     /locus_tag="ACG827_002051"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0484180.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="ACG827_002052"
     CDS             6414..7439
                     /locus_tag="ACG827_002052"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0484181.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="ACG827_002053"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="ACG827_002053"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0484182.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="ACG827_002054"
     CDS             8517..8957
                     /locus_tag="ACG827_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0484183.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="ACG827_002055"
     CDS             8960..9562
                     /locus_tag="ACG827_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0484184.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="ACG827_002056"
     CDS             complement(9625..10728)
                     /locus_tag="ACG827_002056"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0484185.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG827_002057"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG827_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0484186.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="ACG827_002058"
     CDS             12294..12689
                     /locus_tag="ACG827_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484187.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="ACG827_002059"
     CDS             12689..12940
                     /locus_tag="ACG827_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484188.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="ACG827_002060"
     CDS             12983..13378
                     /locus_tag="ACG827_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484189.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="ACG827_002061"
     CDS             13375..14631
                     /locus_tag="ACG827_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0484190.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="ACG827_002062"
     CDS             14643..14966
                     /locus_tag="ACG827_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484191.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="ACG827_002063"
     CDS             14959..15225
                     /locus_tag="ACG827_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484192.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DAZKOU010000068        15102 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000068 DAZKOU010000000
VERSION     DAZKOU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.069"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..666
                     /locus_tag="ACG827_002064"
     CDS             <1..666
                     /locus_tag="ACG827_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0484193.1"
                     /translation="ILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISY
                     FVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASV
                     MGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPR
                     NSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDS
                     ALF"
     gene            678..1598
                     /locus_tag="ACG827_002065"
     CDS             678..1598
                     /locus_tag="ACG827_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0484194.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1639..3096
                     /locus_tag="ACG827_002066"
     CDS             1639..3096
                     /locus_tag="ACG827_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0484195.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3441..4055
                     /locus_tag="ACG827_002067"
     CDS             3441..4055
                     /locus_tag="ACG827_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0484196.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4048..5772
                     /locus_tag="ACG827_002068"
     CDS             4048..5772
                     /locus_tag="ACG827_002068"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0484197.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5769..7220
                     /locus_tag="ACG827_002069"
     CDS             5769..7220
                     /locus_tag="ACG827_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0484198.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7693..9846)
                     /locus_tag="ACG827_002070"
     CDS             complement(7693..9846)
                     /locus_tag="ACG827_002070"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0484199.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9848..10894)
                     /locus_tag="ACG827_002071"
     CDS             complement(9848..10894)
                     /locus_tag="ACG827_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0484200.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11044..12333
                     /locus_tag="ACG827_002072"
     CDS             11044..12333
                     /locus_tag="ACG827_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0484201.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12346..15048
                     /locus_tag="ACG827_002073"
     CDS             12346..15048
                     /locus_tag="ACG827_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0484202.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         5087 a         2638 c         3047 g         4330 t
ORIGIN      
        1 atccttttga atctgctgat ctcagtcgta tttgcaatca ttatgagcga gttgagaaat
       61 aaacgactcg ttaaagttta tcaaacgatg tccttactac cttattttct atcttgggtc
      121 attatcagtt actttgttta cgctttttta agtcctgaca aaggaatatt caatcagtgg
      181 atcactggac atggaggaga agctgtcaat tggtataatg aaccgaaata ttggccgttt
      241 atcttggtgt tcattggtac atggaaaggg attggttata acagcattat ttactttgca
      301 tctgttatgg gaatcgatcc gacttattat gaagctgcta tggttgatgg agctagcaaa
      361 tggcagcaga tcaaacatgt gacgatacca caacttattc cactaatgac gattttaacg
      421 attttagccg taggaaacat tttccgagct gattttggcc tgttttataa cattcctagg
      481 aattccggtg cattatatga tgtgactcaa gttttggata cgtatatcta caatggcctg
      541 acatccacag gagattttgg tatgacggct gctgcaggac tttaccaatc agttgtcggc
      601 tttgtcttgt tgatgatcac aaatacgatc gcccgacgtt ttgatagcga ttctgcatta
      661 ttctaaggag gaaaaaaatg aaaaaaaaga aagtggagag agtccaagtc cgctctttca
      721 ataagacggc taattttttc tctaatataa tgattgcact ctttgctatt tcctgtattt
      781 tgccatttat atttgttatc gccatctctt ttaccgatga gtccatattg tcgactcacg
      841 ggtacagttt ttggccagaa aagttcagta tatttggtta tacgtacttg ttcgaccaga
      901 tgcaggataa aattttccaa gcgcttttcg tcactgtact tgttactgtt ttaggtacac
      961 tgatcaatag cacggctact tctttatatg cttatgcgat ttcacggtca aactttccat
     1021 tccgtcgttt ttttaccgtt ttttgtttga ttacgatgtt gttctcacca gggatggtgg
     1081 ccaattatct tgttatgaca aatcttcttc agttaaaaga tacgatttgg gcattgatct
     1141 tgccaatggc tgtcagtccc ttcaatatta ttgtgatgcg cacattcttt aaacgttcgg
     1201 tctctgattc gattattgaa tcggcacgga tcgatggagc tagcgaactg cgaatatttg
     1261 ttcagatcgt tttaccgtta gctgttccag gtattgcgac aatcagttta tttgccgcat
     1321 tgggttactg gaatgactgg ttcaatgctt tactatacat ccaagatgat aagctagttc
     1381 cactacagta tcttttgatg aaaatccaaa gtaatattca atatctgaca caaaattcag
     1441 gagcaggaag ccaattgaca ggcggattag cctcagttcc aggagaatct gccagaatgg
     1501 cgatcgtagt catctcgacg ttgcctattg cgatcagcta tccatttttc cagaagcatt
     1561 ttgtcaaagg tctaacaatc ggcggagtaa aagaataaag aaacaaaaat ataaatcata
     1621 aaattaggag gaagaaaaat gaagttatgg aagaaactag cctttacagg tgtttcagcg
     1681 ttgttgttgg gaacattagc agcatgcgga ggttctggtt ctaaagacca ggcagaagct
     1741 agcaattcgg acactttgca gatgtatcaa atcggagata aaccagataa tttcgatcaa
     1801 ttgatggaag tagcaaacaa acgaatcgaa aaagagatcg gtgtaaaagt caatatcaat
     1861 tacattggct ggggcgatta cgaaaagaaa atgaacgtca tcatttcttc cggggaaaat
     1921 tatgatatcg cctttgcgaa caactatgtt tccaatgccc aaaaaggagc ctttgctgat
     1981 ttgactgaat tagcgcctaa gtacgctgaa aaagcttata atgatttaga tgaagcatat
     2041 attaaaggaa atctggtcaa tggaaaatta tatgctttcc cagtcaatgg aaatgtcttt
     2101 gctcaacaag tacttacttt taacaaagct ttactagata aatacgatct ttcaattgat
     2161 ggaatccaat cttacgctga tgcagaaaaa gtcttgaagg aattccatga aaaagaacca
     2221 aacacggcag cttttgccat tggacaaggc ttcaaagtcc aagggaactt tgattatcct
     2281 ttagggaata cgttaccatt tgctattgat ctggatggag atgattcgaa aatcatcaac
     2341 caatacgata atgaaacgta tatcggactg ttacgtacaa tgcatagctg gtacaaacaa
     2401 ggactcattc caagtgacgc tgcaacgagc aataaggatt atccactaga aggaaatact
     2461 tggctaatgc gtggagaaac acaaggaccg tatgattatg gcgatacaat tttgacaaat
     2521 gcagcaaaac aagaacttgt ctcaaaagca attactgttc cactaaaatc tacagctcaa
     2581 gcacaaatgg caaattttgt cgtatcaaat acttcaaaaa ataaagaaaa atcagtagaa
     2641 cttctagggc tgcttaacag tgatccagaa ttattgaatg ggcttgtttg ggggatcgag
     2701 ggagaagcat gggaaaaagt cgatggttca gatgataaaa tcaaattgct agatggttat
     2761 cagccaaata cacatatgtc tgcttggaat acaggaaaca acaagattct atatacacaa
     2821 gagtctatta cggatgacat gattgccaaa cgtgatcaat cgattgctga tgcagaaact
     2881 tcaccgattt taggattcag cttcaacact gacagtgtca aaacagaatt aagtaatatt
     2941 tctaatgtca tgaatcaata tttagatgga ttgaatacag ggactgttga tccagatgaa
     3001 acattaccga aattgaaaga cgctttggac aaggctggct atgataaggt attgaaagaa
     3061 atgcaaaaac aatatgatga gttccgacaa gaataataat gaataaagac aaaggggttg
     3121 tgacaaaaga cgtgtcacaa cctcttgttc cgaataaacg gtgttcaaaa gccagtttct
     3181 cggtattaag ctaaatatat gtaaaaatgt ggaaagccat attcacaaaa tttttcttac
     3241 tactcgaaac taagcggcta tttcacaaac tcttggtaaa cggtgttcaa tcagctaacc
     3301 ctcggtattc gctcacaaaa cgacaaaata tgaggaacta tttttccgtt tgtttctcta
     3361 ataatcaggt caaggtgctg attgcccaac ctttttttgt ataatgagaa agtataaact
     3421 taatgtttgg aggagaagat atgttaggga aggttttgga agagttattt atccggatct
     3481 gggtcattat aaaactaacc ttgtatttct ggatatatac atttgccggc gggatcatct
     3541 ttggattagg tgcggcatgg aaaacagtca atgaactatt ttatttgtac ggattcgagt
     3601 ataaagaaat cacaataaaa agaggctgga atatatataa aagaaatttt ttaagaggca
     3661 atctgctatt tagtttattt ttatctggta ctgctttatt gagttacaat ttatatttat
     3721 ctgttcaaat caaagggctc atttttttag ctattgattt tattttactt tttgccttgc
     3781 tctgcctatt tgctacttat caatacagta tgattttaga tagtgaatat acgatctctg
     3841 ttcctaatct gctaaagcta agttttattt cggttttttc cagcttttcg tcttttctaa
     3901 aaataattat tggtagcggt ataattttag gcgtgacttg gcaattcaaa ggattgatcc
     3961 tgtttggtgt cattggtctt ttgacggttt ggaatggtac gatgacaaca cattggcgtg
     4021 aagaattaga taaacagttg gaaagttatg aataagtttc ggattctatt ttcaaaagga
     4081 accttactaa atcgtctgtt gcggaaatac gcggttatca tgatcggtgt aacgatgact
     4141 gctaccgtta tttttagtat ccatacttgg gaacaaaatc aaaaacaggc agaaaacatg
     4201 acaagtgatg ccgttcaaag cacaagcaga atgctgaatg ataaaacaac cttgagtagg
     4261 atcatcaaaa accagcttgt agggaattcc gaaaagatcg aaaatgtgac gacctactta
     4321 acaaaaccca tagatcagta tctgatgtat gtatacgaac agcaaaacag taccgatgaa
     4381 ttagtttctt tcccaaatca aatcaaagat ctctacgcaa attatgaaga actaagtgcg
     4441 atatatatcg tgttgaatca actaccggag tattatgaat cttctagaga aaacaaaggt
     4501 ggggaaataa aaaaggggac accaaaatta ctagacgcct tttatatcaa gttgcctatc
     4561 acacaaggag gagctgagtc aggaagtatt tttattggtt ttcaaaagaa agaactagat
     4621 atgattttag aaaatctaac gtcattcaat ggactctctt tatacatgat ttctggaacg
     4681 acaaatcgac tatacacatt ccatgataat aaaatagcta aagaaacgtt tgaaagacaa
     4741 gaaatgatca tcacagagag tctgaagaaa aattcacaat tgccgattga cgatctggaa
     4801 aaaaacaatt ttgttcaaca ccaagaactg tctgatgatt atcgtatatt agctgtacta
     4861 gataaaaaaa gtgtaaaaat cgaaacggga aaaaatcttg ccccgctgat cattggtatc
     4921 attattttag acaatatact gcttatcttt ctttataaaa cattcaaacg atattcacag
     4981 caagtatcta tcgtcatgca ggccatggat caaacagcag cagggaattt agagacgcgg
     5041 atcgacataa caaacacgga atatgaactg aaggaacttt caatcggtat caacgaaatg
     5101 ttggatagca tcaatcagtt tgtagaagac atctacaaat tagaaatcaa acagcaagac
     5161 gcacatatgc gggcgcttca agcacagatc aatccacact ttttatataa tacattagaa
     5221 tatatccgaa tgtatgcaat cagcgaagga agtgaagaat tagcagatgt tgtgtatgcg
     5281 ttttccgctt tgcttcgaaa caatacgaat caagagaaaa cgatcacttt aaaagaagaa
     5341 ctggactttt gcgaaaaata tgtctatttg tatcaaatgc gttatcctaa tcgagtagcc
     5401 tatcatttta tgatcgatcc tgatttggaa aagatcgaag tacctaaatt tgtgatccag
     5461 ccattagttg aaaattattt taaacacggt attgatttta ctcgattcga taatgcgcta
     5521 agtgtcaaag tcttgcagga aggcaaacgg gtaaggatca tcatcaaaga taatggaaaa
     5581 gggatgactg aaaaacggct aaaacagatt gaagaaaaac tgtctcatcc taaagtggaa
     5641 cttcacggtt ctatcggtct gcaaaacgtg aatgaacgtt tacgtgccag cttcggcagc
     5701 agttattata tgtcgcttga gaataatgag acaggtggac tgaccgtttc tattacattc
     5761 aaggagggat gagcgtgtat caagtgttat tggtagatga tgaatacatg attttgaatg
     5821 ggttaaaaaa gataattgat tggcaaagtt taggctttca aatagtagca acagcagaga
     5881 atgccaaaga aggactagct gtacttgaac agcgacagat tgatttagta gtaacggatg
     5941 tgacaatgcc agaaataaat ggactggaat ttattgaagc ggctcaaaaa gaacgtcata
     6001 attttgaatt tatgatttta tcaggatacc aagaattcga ttatttgaaa ggaggtatgc
     6061 aattaggcgc cgtgaattat ttgatgaaac ccgtgaataa gttcgaattg gttgaaagcc
     6121 tcaagaaaat caaaacacgg ttagatcagc aaaatgaaca aaaaaatcaa caagaaatct
     6181 atcaagaact gttattcagt caatggttga atgaagaatt ggatgaaata agtgaagaag
     6241 aaataatagg acaaataagt gagaagcagc ataggatagt attgattcaa ctggcaagaa
     6301 ctcatgatac tcttatcaat agatggttgc aagaacggca acaaatcttt tactatcaac
     6361 gaaattacgg agaccgtgta ttgatcacgt tgttgttgga ggcagatgaa gttgatggct
     6421 tctgttgttt tgttcaagaa aactttcctg atcaggaatg gctgatcagt atcggggaag
     6481 agacagattc tttggacaaa atccctgaaa cattccaaca agcgaaagac aatctacagt
     6541 tgcatcaatt ttatggagat aaagacgagc atgtttttta cgcagtacaa gcagaaacaa
     6601 aagatcaaac catcgatttt tcaagtttca gacgcgtgct gcaaaataaa caattagaag
     6661 tcgctcaaaa aatgatcact gatttttttg aacagtttca gttagcagcc atgatgccgg
     6721 aagatatacg atattccagt tttttactgt ttatggagat tcaacgggaa ttgattgatc
     6781 tggaagatga ggaatattta caaggaatcg aaaaaatcca tcaggcaaaa acggtgcaag
     6841 aacttcagca gctacttctg tcattcatca aaaagcatca gagacagaaa aagtacagtg
     6901 acaatgtgga aaaagtaatc gtaatcctgc accagcatta tcaagaacca cttacactca
     6961 aagaagtgtc agaaaacctg catttaaatg tcatgtatct aggacagctg ttcaaaaaag
     7021 aaacgaaaaa aagtttttca gcttatttga accatttacg tatggaaaaa gcgaaacaac
     7081 tgcttttgca cagtaatcaa aacatcaatg agatagcaag tgagattggc tacaacaaca
     7141 ccacgtattt ctctaaatta ttcaagaaaa tcgtcggcag atcccctaaa gagtaccgag
     7201 aaaacagtgg agaatcataa aagaaaaagc caatgtaatg aaataactta taaaaaattg
     7261 tctagaaatc taattttggg ctgttcttat tacttgaggc aaggcagctt tttcatgacc
     7321 tctcgataaa cggagttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat
     7381 gtggaaagcc attttcacaa aatttttctt aatactcgaa actaaacagc tttttcacga
     7441 cctctccact aaacggtgtt caatcagctg accctcggta ttagctcaaa aaatggcaaa
     7501 atatgaggaa ctattttgcc atttgtctct ctaatactca ggtcagaacg ctgatttcac
     7561 aacctctcaa taaacggtgt tcaaaagctt gcatctgcgg taataagacc aaccaaacga
     7621 aaaatatgag gatctatttt tgcttggctg ttcttattac ttgatgcagg acagcttttt
     7681 cacaacctct tgttaattat agtttgataa ggaaaaaggc aaatcttttg cttgatattt
     7741 cttagggtga gggatgatcc ctaattggta agagatcgtg ccgcctttca gcaaatcttg
     7801 atgggtaaaa tataatttcg tatattcttt gtccatccgt ttgattttat ggataaattg
     7861 ttgctgcggc cgattaggcg ttgcttcaat cgtcaattcc tttccatttg acaaaagcaa
     7921 aacagccttg tcaattaatg gcataccgat aacatattca ccggttccag gcgtgacagg
     7981 ataaaatccc agactattga agatatacca agcggccata cttccattgt cctcatctcc
     8041 agggtaaccc attggagaat cgtcgaaggt ctgagtcatc aactgtttaa gcagtggctg
     8101 tgccatttca ggttgtccta tataactgaa caagtaagga tagtgaaaac ttggctggtt
     8161 tgaaatagct aattgtccaa attctatcgc ggccatctca ctcatttcgt ggatctcaaa
     8221 accatatcct cctgggtcaa acgttggggg ctgattgcac agctcaatga gtttttcttt
     8281 atatctctca ctgccaccat attttccgat cagtcctgaa aaatcgtgat aaacagcaaa
     8341 gctgctttgc caagcacttc cttctgcata atcttgtccc caacgattag ctgaaaatgg
     8401 ttctctgaac ttgccatctt tatcttttga acgcataaat cctgactcat agtcaaagat
     8461 attttgatag ttcaatgatt gctgctgata tttcttcgtc atttcctctt cttctagtac
     8521 cttggcaact tggctaatac aaaaatcgct ataagcatag tctaatgtgt gattgactga
     8581 ttcgtggtag tgattaggaa catagccata tgtcaagtaa tcttctgttc cttggcgtcc
     8641 ataatttgga tgtttgcttt gaaccgtagc acctttttcc attgccgtta gaaattcagg
     8701 catcaagtct cgtcggatat cttttacagc agcatctgca atgaccgcat cgatcaatgt
     8761 cccaggcata agtccacgtt catcaggaga cagccatttt ggcaaatagc cactttctct
     8821 atagctgttc aaaaaacctt ccagcatttc ttcgtatttt tctacagcaa tcaaagaata
     8881 aagaggataa accgttttat acgtatccca aaacccatta tttgtataga gaacaccagg
     8941 ttttatagat cgactagtcg tatcatagtg gattttttgc ccactaggat caatttcata
     9001 aaatgtttgt ggaaataaaa acacccgata taaattatga taaaacgtcc gcaattgttg
     9061 ttgatcatgg tgtgtgagct gtatacgatc aaaataggac tgccattgtt cttgactgtg
     9121 agcaaggtaa gtctctttgc tttggttttg ttctagttcc aggttataag ctgcttgttc
     9181 cgagctgata aaagatgtcc caaatcggat agtttgtttt tttgtctgtt caaaatacaa
     9241 cgggatcgag tcgctatttt tgttggaaac agctaactca tcaattgtta aaggatattc
     9301 aaatctcaat ataaaataaa aagaaaaatc aggatcttct gaaccagcaa aatgtgtgat
     9361 ttttccttcc actgtgtaag gatctataac aaagagctga tggcgtcccg gaaaggataa
     9421 aagtaaaccg ctgtcatttt tgctgtaatc aatagataag attccgccat acatgctagg
     9481 tatcaatgta gaacagatgc cataacgtaa ttgacagatc gacagatgag tcgggcaaaa
     9541 tatactttct tctgggcggt aagaactttg agcatggaaa agggtgttct cctgtagttt
     9601 tccattgatc ggcgtaaaca gcatatgact gaaatcaccc atccaaggac ttggttgatg
     9661 ggtaagacgg tagccttgaa aaacgcggtc atcaggatga aaccaccagc tgccgttttg
     9721 atccgtagtt tgcggtgcaa aataattcat cccaaaagga acgccagtat aaggctggca
     9781 gttcccatta gagaaatttg gctgattcgc tgttgcatgt ctagtatcta tttgaccgat
     9841 attcatttta tttctccttt gttgattgtc ttttgatgaa tgaaactgga aatttactat
     9901 cggatggtcg atcttttgtc tctaaccaag tgaccaaagc atctcccgca agcttaccta
     9961 actgttcaaa atcttgtgcg atggttgtca gtggcgggtc aacaagagct gctgcttgga
    10021 tatcatcaaa tccgataatt gaaaaatctt cagggatatg ataaccatgc tgtctcaaga
    10081 gattcatggt ctggatagcg acaacatcgt tttcacaaac aaaagcagtt acttcatact
    10141 tagtgatata agaaagcaat tcatctaatg tcgcgctttt gtcatccagt gaagtgtgga
    10201 aagtcaaacc agctttgtcc aatgcttcaa ggtaaccaag gtaacgctgt cgaacggatt
    10261 gtggatgagt ctgcgtacca aacaaataag cgatttttga atgtccctgt tcgatcaaat
    10321 aagaggcagc aagcgcaccg ccttggaaat tatcggaaag aacggtagga aaaggtaact
    10381 catagatttt tttatctaat atgacgacag ggaattcttt caatgaaagc tcaaaaagta
    10441 agtcaagatg ttgttctgta tcaagagcat aataaatcaa tccgtcaaat tcattgatta
    10501 gctcttttgc tgttttattt tgtagaaagt caagcgtcgt catcatgaca tcaatctgtt
    10561 tatcttgcat cacagggttc aatcccttgc taaaatcacc aacagacaaa tcagcgacat
    10621 atggaagaag aaacagaatc cgtcgtgttt ttgtaggaga agcaagaatc tgttccggag
    10681 aagcggattt aacgaaactg cctttcccgc gaacgcgata aataagtcct aattgctcta
    10741 attcagtcaa tgcacgtttg gaagtgattc tgcttacttt ataggtacgt gaaagttctt
    10801 tttcagttgg tagttggctg tctacaggta attcaccagt taaaatagcc gtttgcaaat
    10861 cattaaatat tgtctggtac aatggttgat tcatataaac acctcttaat gatatatcat
    10921 attcaaataa taccataatg gatgaaaaag taaataaaaa gatatttttt gaaagcgtag
    10981 acataattgg ctgtatgaag tataatcttt aaagatatat caaatagaaa gaaggtttta
    11041 ttagtggctt ataaagatat accaaaatcg gtgcagaaat ttatggaaga tatcacacaa
    11101 aaatgtgggg atatccatgc ggactgggca aagaatttta cagcagcatt tgccaatact
    11161 ctgttgacaa cagtcaaaaa gcaaaatgac ggaaccacct ttttattgac gggagacatt
    11221 ccagcgatgt ggttaagaga ctctactgca caagttcgtc cttatttagt gattgcaaaa
    11281 gaagatgagg aattagctga catgatcagc gggttagtca aaaagcaatt tttttatatc
    11341 aatatcgatc catatgcgaa tgccttcaac gaagaagcta acggtgctgg ccaccaaaca
    11401 gatcatacaa aaatgaacga ttggatctgg gaaagaaaat atgaaattga ttctttatgc
    11461 tacccaatac agttggccta tcttctttac aaaaatacag gaaaaactga tcaattcaat
    11521 caatctttta ttgaaggtat caaaaaaatc ttggacgtgt tcaaaacaga gcaagatcat
    11581 gaacactctc cttatacatt tgtacgagac acgacaagaa aagaagatac gctgatcaat
    11641 gacgggcgag gtgcaaaaac agcttttaca ggaatgacat ggtctggttt ccgtcctagc
    11701 gatgatactt gccgttatgg atatctggta ccatccaata tgtttgcggt ggtcgtgtta
    11761 ggttatgtgg aagaaatttt tcaaacgata ctcgatgata aagaaattgt acagcaaaca
    11821 cagaaattga agaaagagat ccaagagggg attcagaatc acggacgaac ggttaatcaa
    11881 aaagggcaaa cgatttatgc atatgaagta gatggaatgg gaaacgccac agtcatggat
    11941 gacagtaatg tgcccaatct gatcgccgca ccttacttgg gatattgttc tgaacaagag
    12001 gaagattatc tgcaaacaag aagaacattg ttgagtaaag aaaatccata tttctatgaa
    12061 ggaaagtacg caaaaggaat tggatcctcc catacgccag aaaaatacgt ttggccgatt
    12121 gcattagcta tggaaggaat gacaacgtca gacaaatctg aaaaagagcg gatattgaat
    12181 ttacttgtag caactgacgc aggaacacac cttatgcacg aaggattcga tgtgaacaat
    12241 cctgaaaatt atacgagaga atggttttct tgggcaaata tgatgttttg cgaattagtc
    12301 atggattact ttgatattag agtagaaaag taggaggtat cgtaaatgaa aaagaaagta
    12361 tatatcattt ctcatagcca ctgggatcgt gaatggtaca tggcttacga acagcatcac
    12421 atgcgactga ttgaattgat agacgatcta ctagagctat ttgaagttga tccgtccttc
    12481 aacagttttc atttggatgg gcaaacgatt attttagatg actatcttca agtaaggcca
    12541 gaaaaaagac aggcaatcca acaagcaatc aatgaaggaa aattgagaat tggaccattt
    12601 tatatcttgc aagatgattt tcttatcagc tcggaatcga acgtgagaaa tatgctgatt
    12661 ggcatggaag aaagccgtaa atggggaacc cctgtcatgt taggctattt tcccgataca
    12721 tttggaaata tgggacagac gcctcaatta atgaaacaag ctggtatttc agcagcagcg
    12781 tttggccgtg gtgtaaaacc aattggcttt gataatcaag ttttggaagc agaaaactat
    12841 tcttcccaat actcagagat gtggtggaaa ggacctgatc aaacagcaat ctttggtctg
    12901 ctatttgcca attggtatag caacgggaat gagattccag tagaaaaaga agcagctctt
    12961 gccttttgga agcaaaaatt agcggatgca gaacaatatg cctctacgaa ccacttattg
    13021 atgatgaatg gtgttgatca tcaaccggta caaaaagata tatcaaaagc aatccatctc
    13081 gcaaatgaac tgtttccaga ttacgagttt attcattcta attttactga ctatctggaa
    13141 gcagttcaaa aagatgtgcc agaagactta ggctcagtag aaggagaatt gacaagtcaa
    13201 gaaacggatg gctggtatac tttagccaat acagcatctg ctcgggttta cttaaaacaa
    13261 tggaatacca aggtccagcg acagctagaa aatattacag aaccacttgc gacgatggct
    13321 tatgaagtta gtggaaacta tccccatgat caattagatt atgcttggaa gacattaatg
    13381 caaaatcatc ctcacgacag tatttgcggt tgttcagtcg attctgtcca tcgggaaatg
    13441 atacctcgtt ttgaaaaagc agatgaagtt ggaaagtatc ttgcacaaga ttccttagaa
    13501 cagctgaccg cagcaatcga tacgaccgga ttcccaaaag acagctttcc atttgtcatt
    13561 gtaaatactg caggaatgga taaaactggt gaagcagaaa taaccatcga attagaaaga
    13621 aaaagattcg ctgaaggaat acctgaacaa ctatatcaag agttagaaaa tttgcctaaa
    13681 cgtaagtatc atgtagaaac aaaaagtggg gccactatac cagctatttt atctgaagaa
    13741 actgtccaat ttggttatga tttgccgaaa gaccgtttcc gtgtgccata tatggcaaga
    13801 atgatcaaag tgactttacc attagaaaac atgccagcat tttcttggga aacattcgcg
    13861 cttgtagaag gggaagcgaa agcagaagaa aaggaaacaa tgattcatca gagtggacga
    13921 atcatcgaaa acggccctct acatttgacg attgaaaaaa atggtaccat tacaatggaa
    13981 gatcgcaaaa acaaacgaaa gttgaataat ctgcatattt ttgaagatat cggcgatata
    14041 ggaaatgagt atatctttaa acaacctttc tgtgatcagc caatcctatc cagtaacaaa
    14101 gaaaactctg aagtaaaagt attagtagat acaccggaaa tagcaaaaat aagtattcta
    14161 caggaaatgg aaatccctgt ttctgcagac gagcgtttag aaaaagaaca gcagatggtt
    14221 gtggaatttc gttatcgaaa agcagaacga tccaaagaaa aacgaatcct tcaaataaaa
    14281 acaatcatga caatcagaaa agatagtaaa aaaatcgact ttgaaacgac tctcgacaat
    14341 cagatgaaag atcatcgctt acgtgtcttg tttcctacaa agttacatgt ggaacatcac
    14401 gaagcagata gtatttttga agtggtgaag cgcccaaacc atgtgtctaa aagttgggaa
    14461 aaccccacga atccgcaaca tcagcaagca tttgtcaata ttcatgatga agagtacggt
    14521 gtaacagtcg gaaactttgg tttgaatgaa tatgaagtaa cagaagacgg acaaatcgca
    14581 gtgacgctac ttcgcagtgt gggagaacta ggcgattggg gatattttcc aacaccagaa
    14641 gcacagtgtc taggtgagca tcggttcaat tacagcattg aactacatgg accagaagaa
    14701 aaattctcta cttatttgca tgcatacgca gcacagattc ctttttccac tcaacaaatc
    14761 aaacatcatg aaggaacatt gatttcaaaa caacaatatt taacgataaa aagtgaaacg
    14821 tttgcaatta cagctttgaa acgcagcaag tttagtgata aagtagtagt aagaggtttc
    14881 aatatgtcta gtcatttaga aaaattggaa ataactaaag acaatggaaa aactgtcata
    14941 ttgaatctgc ttgaagaacc aacgaagcaa gcggttgtcc caataatcca accttatgag
    15001 attcggacaa tcggattcga ggaggaaaac gaatgtatgg cggaatagaa gcaggcggaa
    15061 caaaatttgt gtgtgcggtt tcagatggtg gagaaatttt ag
//
LOCUS       DAZKOU010000069        15072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000069 DAZKOU010000000
VERSION     DAZKOU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.070"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACG827_002074"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACG827_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACG827_002075"
     CDS             706..1734
                     /locus_tag="ACG827_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0484203.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACG827_002076"
     CDS             1731..2489
                     /locus_tag="ACG827_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0484204.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACG827_002077"
     CDS             complement(2575..3315)
                     /locus_tag="ACG827_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0484205.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACG827_002078"
     CDS             3376..4629
                     /locus_tag="ACG827_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0484206.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACG827_002079"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACG827_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACG827_002080"
     CDS             5300..5626
                     /locus_tag="ACG827_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0484207.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACG827_002081"
     CDS             5694..6407
                     /locus_tag="ACG827_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0484208.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACG827_002082"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACG827_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACG827_002083"
     CDS             7690..9555
                     /locus_tag="ACG827_002083"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0484209.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACG827_002084"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACG827_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACG827_002085"
     CDS             11120..11821
                     /locus_tag="ACG827_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0484210.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACG827_002086"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACG827_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12857..13066)
                     /locus_tag="ACG827_002087"
     CDS             complement(12857..13066)
                     /locus_tag="ACG827_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFQ0484211.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13307..13882
                     /locus_tag="ACG827_002088"
     CDS             13307..13882
                     /locus_tag="ACG827_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0484212.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13882..14322
                     /locus_tag="ACG827_002089"
     CDS             13882..14322
                     /locus_tag="ACG827_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0484213.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14338..15039
                     /locus_tag="ACG827_002090"
     CDS             14338..15039
                     /locus_tag="ACG827_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0484214.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5083 a         2501 c         3179 g         4309 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    13201 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    13261 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    13321 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    13381 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    13441 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    13501 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    13561 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    13621 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    13681 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    13741 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    13801 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    13861 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    13921 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    13981 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    14041 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    14101 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    14161 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    14221 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    14281 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    14341 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    14401 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    14461 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    14521 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    14581 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    14641 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    14701 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    14761 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    14821 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    14881 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    14941 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    15001 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    15061 gaaagaaaaa tt
//
LOCUS       DAZKOU010000070        14805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000070 DAZKOU010000000
VERSION     DAZKOU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.071"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACG827_002091"
     CDS             <1..135
                     /locus_tag="ACG827_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0484215.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG827_002092"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG827_002092"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0484216.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="ACG827_002093"
     CDS             complement(2412..2663)
                     /locus_tag="ACG827_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0484217.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="ACG827_002094"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="ACG827_002094"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0484218.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="ACG827_002095"
     CDS             3613..3831
                     /locus_tag="ACG827_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484219.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="ACG827_002096"
     CDS             complement(4120..4632)
                     /locus_tag="ACG827_002096"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0484220.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="ACG827_002097"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="ACG827_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0484221.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="ACG827_002098"
     CDS             complement(7085..7528)
                     /locus_tag="ACG827_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0484222.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="ACG827_002099"
     CDS             complement(7529..8317)
                     /locus_tag="ACG827_002099"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0484223.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /gene="rnz"
                     /locus_tag="ACG827_002100"
     CDS             complement(8314..9255)
                     /gene="rnz"
                     /locus_tag="ACG827_002100"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0484224.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="ACG827_002101"
     CDS             complement(9432..10019)
                     /locus_tag="ACG827_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0484225.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="ACG827_002102"
     CDS             complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="ACG827_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0484226.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="ACG827_002103"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="ACG827_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0484227.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="ACG827_002104"
     CDS             complement(12936..13871)
                     /locus_tag="ACG827_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0484228.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14057..>14805
                     /locus_tag="ACG827_002105"
     CDS             14057..>14805
                     /locus_tag="ACG827_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0484229.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT         4169 a         3277 c         2607 g         4752 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataacc cgcgaattgt aaaattaagt
    13981 tagaaaaata aaaaggcatt tatggtacac tcaaattgta tttccgacga aagaaaacaa
    14041 aggagtgtaa ccataaatga cctacaaaca tcttaccata gacgaactga caatgataga
    14101 atcatattat cttcaacata ataagccggt tgaaatcgct aaccgaatgg gacgtgctat
    14161 acaaactatt tataatgtag tcaataagtt caagcaaggc aagactgctc ttgattattg
    14221 gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa gtcattcaat tacctgctca
    14281 tgaagtagat tacattaaag agaaagtcac tcttggttgg acgcctgacg tcattatcgg
    14341 gcgaaaagaa aggtctgttt catgcggtat gagaacactt tatcgtttat tttctaaagg
    14401 aatatttgat attgacacac taccgatgaa aggtaaaaga aaacccaatg gccatcagga
    14461 aaaacgggga aaacaacagt atcagcgctc aatccatgat cgacctgata attatcctga
    14521 tttcaattct gagtttggtc accttgaagg tgatacgatc gttggcattc atcataaaag
    14581 tgccgtcatt actttagttg aaagattatc taaagtcatt atcacgatta aacccaacgg
    14641 ccgtaaggca ttagatattg aaactgccct taatcaatgg ttttctcgct tccctaaaaa
    14701 cttctttaaa tctattacgt ttgactgtgg aaaagaattt tctaactgga aagccattag
    14761 taaccaacat gatattgata tatattttgc ggaccctgga acacc
//
LOCUS       DAZKOU010000071        14625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000071 DAZKOU010000000
VERSION     DAZKOU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.072"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG827_002106"
     CDS             4..816
                     /locus_tag="ACG827_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0484230.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG827_002107"
     CDS             813..1613
                     /locus_tag="ACG827_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0484231.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG827_002108"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG827_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0484232.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG827_002109"
     CDS             3110..4819
                     /locus_tag="ACG827_002109"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0484233.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ACG827_002110"
     CDS             5031..9389
                     /locus_tag="ACG827_002110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0484234.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ACG827_002111"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ACG827_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0484235.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ACG827_002112"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ACG827_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0484236.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ACG827_002113"
     CDS             11227..11520
                     /locus_tag="ACG827_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0484237.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ACG827_002114"
     CDS             11517..11828
                     /locus_tag="ACG827_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0484238.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ACG827_002115"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ACG827_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0484239.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLQEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG827_002116"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG827_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0484240.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4982 a         2599 c         3255 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgcaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DAZKOU010000072        14310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000072 DAZKOU010000000
VERSION     DAZKOU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.073"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..195)
                     /locus_tag="ACG827_002117"
     CDS             complement(<1..195)
                     /locus_tag="ACG827_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0484241.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNG"
     gene            473..793
                     /locus_tag="ACG827_002118"
     CDS             473..793
                     /locus_tag="ACG827_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484242.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            1245..1445
                     /locus_tag="ACG827_002119"
     CDS             1245..1445
                     /locus_tag="ACG827_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0484243.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1592..4381
                     /locus_tag="ACG827_002120"
     CDS             1592..4381
                     /locus_tag="ACG827_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0484244.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4428..4955
                     /locus_tag="ACG827_002121"
     CDS             4428..4955
                     /locus_tag="ACG827_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0484245.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4976..6166
                     /locus_tag="ACG827_002122"
     CDS             4976..6166
                     /locus_tag="ACG827_002122"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0484246.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            6206..7504
                     /gene="asnS"
                     /locus_tag="ACG827_002123"
     CDS             6206..7504
                     /gene="asnS"
                     /locus_tag="ACG827_002123"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0484247.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8299..8973
                     /locus_tag="ACG827_002124"
     CDS             8299..8973
                     /locus_tag="ACG827_002124"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0484248.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8970..9311
                     /locus_tag="ACG827_002125"
     CDS             8970..9311
                     /locus_tag="ACG827_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484249.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9341..9700
                     /locus_tag="ACG827_002126"
     CDS             9341..9700
                     /locus_tag="ACG827_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0484250.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9960..10082
                     /locus_tag="ACG827_002127"
     CDS             9960..10082
                     /locus_tag="ACG827_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484251.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            10224..12308
                     /locus_tag="ACG827_002128"
     CDS             10224..12308
                     /locus_tag="ACG827_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0484252.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12619..14085
                     /locus_tag="ACG827_002129"
     CDS             12619..14085
                     /locus_tag="ACG827_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0484253.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4737 a         2498 c         3024 g         4051 t
ORIGIN      
        1 cccatttttc actacaatta ctttcccgtt tccatctacc acagtagaac cacaatttct
       61 acaggtatgg ggagcaggtg agaaaacagc atcgacgatc aacgtctttt tcttctgaaa
      121 ggtctcgtaa gagacctctg taatcatcaa atctttctct attaatcgca gcattttttt
      181 gatagaatca ttcatatagc atatcgtcct ctcagttgtt tattttgtcg tgatttaatc
      241 atactagaga acgatatgtt ttttaatacc taaaataaaa atggggctga agaatcaatt
      301 ctgattcatc agtcccataa attatagagc ctaaaaaaga ggttgtgacg tggtcacgac
      361 ctctttttga cgattatgat acaaaaaaca tcatttatga acaaaatgtg aataacgggc
      421 gttcttgaga tatgatttta attgggaaaa acttatcgat aaggaggtct ttatgaaaaa
      481 aagactgtta tggtgtttgc tgtttctatc cctaagttta acaagtcagc cagtttttgc
      541 tgaagaaaca cagcggtctc ctattacagg cacacgtctg tacaaaaatg tttatgtaca
      601 gacaacagat cttgattctg cgattcctga aacagaagaa cggtctttgc agccaagatt
      661 tggttacttt tggtactgtc cgacctgtga ttatgaaagt tgctggcatt tcttacattc
      721 tacagctgca gcttttgctt ttactcatac tcagaaatat gatggacatc atcctgttat
      781 ttatgaggta tagatatgag caaaaaagaa aaactaatct cgttgatcgt attggtttgc
      841 cttgttcttc tggtaactgt cataggctat cagaaatacc atgagaacat cagacctact
      901 aaagaatctt ccccagcctc gaccgttgaa acagaaccga taagtagtca gaaagaataa
      961 tttttttagt ttaaagaaga acatcagagc aaaaaaacac caactgaaca atatgtcgtt
     1021 tattttaaaa aaagttcggt ttttcacttt atgtaatcgc tgagaaaact ttttttaata
     1081 aaaaaagtcg aaaaactcta gcttttttgt aaacggtatg atatactata ggagtagttt
     1141 ttgttcggta atctgtggta gcttgtttca aataaacatc ttccagatat acaccattca
     1201 agtaattgca aaatatgtgt tgatacacaa ggaggatttt tattatggaa acaggtacag
     1261 taaaatggtt taactcagaa aaaggatttg gattcatcac tgcagaaaac ggaaacgatg
     1321 tattcgttca tttctcagct atccaaggcg acggattcaa aactttagaa gaaggccaag
     1381 cagtaacttt cgatgttgaa gaaggccaac gcggacctca agcaacaaac gttactaaag
     1441 cttaattaac ctatactaaa aaataaacgc ccagacattt gcgtgtctgg gcgtttttgt
     1501 ctattttctt taaaaagcga tgagagtatt tttctgcact ttgtagtata atttcaaaag
     1561 aacattagag gcaggagaag aggaagatat tttgaaacca agaacttatg cagtcgttga
     1621 tatcgaaacg actgggacga atccaaaaga agatcggatc atccaatttg gctgcgtcat
     1681 gattgaatca ggtcggatca cttcacgttt ttcgatcgat attcatccag ggcggaagat
     1741 ttcaaaacaa atccagcatt taacagggat cacgaatcaa cgcgtccaaa aagcgcctta
     1801 tttcgaagat gtcgcccaaa cgatttacaa tcttttggca gacaccatat ttgtcgcgca
     1861 taatatttat tttgattata attttttgaa ccatgaactc atgcgatgcg gacttcctag
     1921 tttgaagata ccaggcatcg atacagtaga acttgcacaa attttcttgc cgacggaacc
     1981 aagctttcgc ttagctgatt tgtcagaaag ccttggattt attcatgaga atcctcatca
     2041 ggcagatagt gatgccgaag tgacgggtca acttcttttg ctgatcgaag aacggatgag
     2101 aaaattgcca atcatcacgt tagagcagat tgcgcgatta agtagacaca caggtatgga
     2161 tacgagtcgt ttcatttatc atgtgattga agaaatgaaa gaaaaaccag agccacttga
     2221 tccctcatta gaaatcgtcg atgggctagc tttgcagaaa aaagaagtgg aactattcac
     2281 atccgtccat tatggcgagc ggacatatcc aagaaaaaaa caagcaaaag aaaaaatgtt
     2341 tggtaaaaca ctgatgtatc gaaaagaaca gaacagactg atgaatgcgg tctacgacca
     2401 tttcacaaaa gatgaatcaa aagatttgat gatcgaagca gctacaggaa tgggtaaaac
     2461 aatcggttat ctgctgccat tgagttattt ggctacacct gaaaaacccg ctgtcatcag
     2521 taccgtttcg ctagttttgc agcagcagat catagaaaaa gacattccat tgctgaatca
     2581 attgctagat cagcccatac aacctgtaat tatcaaaagc tatcgccact atattgattt
     2641 acagcgtttt aaaggcacac ttgtggaacc gccagaacaa aaacaatatg ctttgtatca
     2701 aatggcggtc ttagtttggc tgacgcaaac gaagaccggt gatttggatg aattacattt
     2761 aacaaatttg aaccattcat tctttgcaga tatcgcgcac agaggtattg gatttttagc
     2821 aagaaatcag tcattttacg aacaagactt tgttcgtttc ttgcaaaaga aaatcaggca
     2881 aagtaatttt ttgattgtca atcatgcgtt tttaatacaa gaaacacagc gaaaagaacc
     2941 attgcttcca gctagtccat ttttgttgat cgatgaagta catcatttgc cggaaatcgc
     3001 tgaaaaagta aatgacaaac gattaagtat taactatttc cataaacaaa tccagcaatt
     3061 agaagaacca aatcagctgt ttgaccgaat acaaagctat ttaccgaaag aacatgaagc
     3121 cttacgctgg ttatcgattt atcgagaaga actttctgcg ctatgcgaag cacagatttg
     3181 tatggcagaa ggattaatga gtttagtgag cggcaagcaa cctaacggac agtatccaga
     3241 agaagtcatg atgacaaaag agatgttgga tcagctgcca ctggaatctg ggacagccat
     3301 tgatcacctg cagctttttt atcaggagat ccagcagcta cagtcacaaa tgcgtctgac
     3361 ttttgagatt atccaagaaa gctggacaaa ccagatgcgg atcgatttag caagtttata
     3421 tagcttattt gatcaattag acaagcagta cttgctgatg gatcaatggc tggaagactg
     3481 gcgagatcat ttgatccatt ggttcgttcc tgggaacaca gcgcagcaga tgatttttca
     3541 tctccatgat tttcaagcgg ctatgttgcc gtcgactgtt tggtatcctc gatatgatcg
     3601 tattctttat attggaggaa cgctgaagat cggccctaat cgacactatc tctctaagcg
     3661 gcttggcatt gaagcagcac ctcttaaagt gattgcctct ccttatgatt atgagaaaca
     3721 ggcaaggatc tatgtgccaa aagaggcacc tgccgtccat caagtcagca atcaagcata
     3781 tatcgattat ttagagacag cacttacagg tctgatcgaa caagaagatc ggccaatact
     3841 tgtgttattc acttctcatg atgttttgca gaaggtctat cagaaaatgc atctgtctat
     3901 gctagaaaaa ggacgagaaa tactagctca agggtttggc ggaagccggg agaagctttt
     3961 gaaacgcttc attttgtcag aaaaaagcgt attgtttggc gctgacagct tttgggaagg
     4021 tgtagatttg cctggggatg ctttgcagat cgttgtagtc acacgtttac ccttcgataa
     4081 tccgcttcgc ccgatggtca aagctaggaa cgcttattta gaatcagagg ggatcaatcc
     4141 tttttaccag gaatccgtac ctaaagcagc tttgaagtta cgccaagcat taggtcgctt
     4201 gataaggacg gagacagatc gtggcgtatt gcttattttg gatcgaagac tagtcactgc
     4261 aaaatatggt gcacggatca tccatgcatt accgaaaaaa gtaccgatca aagagctagc
     4321 tatggaggaa atctatcagg acatccatga attcttaaag aaaaatgaag aaggaagtta
     4381 gaggagctgt cttttttcat aattaaaatc tgctataatg aaaaggaatg aagataaagg
     4441 agcgacgacc cttgaagaaa aaacagacaa aggaaaaaac gattaatcgc gtgttacttg
     4501 gcttgatcgt atttttatta gcggtcatcg tgatcagctc gattttttac ttgcgttcca
     4561 gtcgtccggc ggtgcaggcg aagaaagaag cagtagaaat cgcaaagaaa tatgcaaatc
     4621 ttgaggaagc cgatcatttt tattggttta ctcgaaagaa gacatacttt tcactgacag
     4681 gtacaaatga taaaggaaaa gaaatcgccg ttatcattcc taaatctggt gaaaaagtaa
     4741 aagtattgga tcaaacaaag ggactgactg aaaacgaagc aaaacagaca attgctgcgg
     4801 cccatcctga aatccaaatc gaaaaagctg ctttagggat gtatgatgat cagcctgtgt
     4861 gggaagtagt aggaaaagaa acaaatggga aactgaatta ttacctgatt ggctttgaat
     4921 ctggagatga agtcaagaca atcaagggaa tttaaaaggt aaaggaatga atgggatgga
     4981 gttatcaaaa agaacaaaac gattagaacc gtctgtcact ttggcagctg cggcaaaggc
     5041 caaggcatta aaagcaaaag gcagagatgt gctgagtttg actgtgggag aaccggattt
     5101 tgcgacacca gaaaacatcc aagaagcagc aatcgaagca atccgcaatg ggaaagctag
     5161 ctattataca ccaacagcag gaatcccaga gcttcgacag gcaatcgttg attatctgaa
     5221 gaaaaactat ggacttgtct atgaaccttc acaaacagtc gtaacagacg gagcgaaatt
     5281 tgctttgtat acccttttcc aaacgatcct tgacccgcaa gatgaagtca tcattcctgt
     5341 gccttactgg gtcagttacg gtgagcaagt aaaactggca gaaggacttc ctgtctttgt
     5401 gaaaggggaa gaatccaata gctggaaagt tactgttgaa cagctagaac aagcacgttc
     5461 agaaaaaaca aaagccgtaa tcatcaattc tccatcgaat ccgactggaa tgatttatag
     5521 caagaatgaa cttcaggcaa tcggtgaatg ggcagtaaaa aatgatattt tgatagttgc
     5581 tgatgatatt tatggtcgtt tagtttataa tggcaatgag tttactccaa ttgcaacgat
     5641 ttcagaagca atcaaaaatc agaccatcat catcaatggc gtgtctaaaa catatgcgat
     5701 gacaggctgg cgcattggtt atgcggtggg gaattctgag atcatcaatg gaatgattgc
     5761 tgttgcttcc cagtcaacca gtaatccgac agcagtcagt caatatgcag ctgttgaagc
     5821 attgaaaggt gagcaagata cagtagaaga aatgcggaac gcctttgaag aacgcctgaa
     5881 tacgttatat ccgcttgtgg cagaactgcc aggtgtttct cttgaaaagc cgcaaggagc
     5941 tttctatctt tttcctaatg taaaagaaac gctacgcatg tgtaaatatg aaaatgtcac
     6001 acaatgggta gaagatcttt tagaagaaac aggggtagcg cttgtgactg gcgaaggctt
     6061 tggggcacca gaaaatgtcc gcatgagtta tgctactgat cgcgctacgt tagaagaagc
     6121 ggtacgcaga atcaagcaat ttattgagag taaaagtcaa aattaatcct gaagaaattc
     6181 aaggaatata aaaggagagt catacgtgga acaaattcaa atcatcgatt cgaaaaacca
     6241 tgtgggagaa acagtaacaa tcggcgcttg ggtcgcaaat aagcgatcaa gcggaaaaat
     6301 cacattttta caattaagag acggaacagc ctttttccaa ggtgtagttg taaaaagcga
     6361 agtgtcggaa gaagttttcc aaacagctaa aaatctacac caagaaacat ccgtgttagt
     6421 aactggagaa atccgtgaag acagtcgttc aaaatttggt tatgaaatcg gaattacaaa
     6481 tgtggaagtg gtaagcgaga gccacgatta tccgatcaca ccaaaagaac acggaacaga
     6541 cttcttgatg gatcatcgtc atttatggct gcgttcttca agacaacatg cgattatgca
     6601 gatcagaaat gaaatcattc gtgcaacgta tgaattcttt aatcaacatc attttgtcaa
     6661 aattgatccg ccgatcttga ctggttctgc accagaaggc actacggatc tattcgaaac
     6721 aaattacttt gatcaaaaag cttatttatc tcaaagtggc caattataca tggaagctgc
     6781 tgctatggct ttcggaaaag tcttttcatt cggtccgact ttccgtgctg aaaaatcaaa
     6841 aacacgacgt catttgatcg aattctggat gatcgaacca gaaatggctt tcactcatca
     6901 agaagaaagt ttagaagtac aggaacaata tgtagcattc ttagtacaaa atgttttaga
     6961 tcattgcgaa tacgcattag atgtactagg acgagacaaa gaagtcttaa aaacttatac
     7021 aaaattgcca ttccctcgta ttacttacga tgatgcaatc gaattactga agaaaaatgg
     7081 atttgatgac atcgaatggg gcgaagactt tggttcacct catgaaacgt tcattgcgaa
     7141 ttcattcgaa cagcctgtct ttatcctaaa ctatccaaaa gccatcaagc cattctacat
     7201 gaaaccacat ccaacaagag aagacgtggt aatttgtgca gatatgatcg caccagaagg
     7261 ttacggtgaa attattggcg gaagcgaacg ggcaacggac tatgatttct tgctagaaca
     7321 aatccgtaat cacggtttgg atgaaaaaga atattcatgg tatcttgatc tacgtaaata
     7381 cggttctgtt ccgcactctg gttttggtct aggtcttgaa cgaacagtca cttggattgc
     7441 tggtatcgac catgtacgtg aagctattcc attcccacgt ttgttgaacc gaatttatcc
     7501 ataagaggtt gtgcgcctgc cgttcagctc cgagtcacaa ggagtaattt caaaaaacag
     7561 tttttcatgt ttgatgacta ttgtgacttg tggccggaga gcaacgttta tttaaaaaaa
     7621 agaactgcga actctcgcag ttcttttttt taaatacggg ataatggaag tttccagcag
     7681 acaagagaag aaagcaaaag aaattgctat ttgattcaca aggttctata acgaaatcgc
     7741 aatcaatcaa acatttacaa agaaggtaat tcaaatggaa atctgatttg atgatgcttc
     7801 tgctgttgaa ataaagcagc atctagcacc tgaaaacaaa ctaatagtaa cattcgaaga
     7861 tggagtaggt ccgtattttc agcatgcaat aatccatatg taagttcaat tcacccttga
     7921 aatcatcgga ccagataaag ttatgaccaa tctatcccaa gcaatctaga agagatatta
     7981 gtaaagggac atttactaga agtcttagat gagcctataa atatatgttt cgatggcgtc
     8041 atcagtggtc tgattgacgg aagcttgggt gtgatcgatc tctctggacc aaacaacgaa
     8101 ggaatcaagg ggaatccggc acgttaagaa aaaaatagca taaaaaacta gacacttgat
     8161 gagcgttcat tcaattgttt gaaggaacta ggctggtgtc attttttata catatatttt
     8221 ctctttcgtc ttgatagggc ttatgggata atataaggaa gaaaatcatc gttttattca
     8281 aaaaggagtt tttgacaaat gaaaaaacgt atattattta tcggtgatag cattacagac
     8341 gcaggaagat accgagcaga agcaactgat ctaggtaaag gttatccact tttgattgcc
     8401 ggaaaactaa tctcccaata tccaacgatg gaatttgaat gcatgaaccg tgggatagga
     8461 ggagacaaaa ttgcagatct gcaagtacgc tggcaagaag attgtttagc tttacaacct
     8521 gactatgtat cgattttgat cggaatcaat gatacctggc ataatactag cgatcaggct
     8581 atattcggaa ctgagaaatc tgctcagcaa tttgaatccg tttatcgaaa attactaaat
     8641 gaacttcaaa cagcggaaat ctgcaacatc atcttgatgg agccgtttgt acttcctata
     8701 accgacgacc gtcatggctg gcgtaaagac ttagacccta aaatccaaat tgttcgtaaa
     8761 cttgctaagg aatatggcgc attgctggtt cctttagatg gtatattcaa tgctttaggg
     8821 attgcttatg gtttttcgaa atatacaaga gaagatgggg tccacccaac gattggcgga
     8881 catgagatca ttgcacaaag ctggatagat gcaattgtcg cagtcgattt ttttagtgga
     8941 gataaaaatg agcgaaagaa aagtgaaaaa tgatggaaat aaggtgggca gtaaaagaag
     9001 atttacctca aatcaatcag ctgattactg atcatccaga atcattcgta aaagcaaaaa
     9061 cggcagctga cctaaatgat aagaataaac tattgctcgt tgctgtaaca aaagaaaaac
     9121 ggattatcgg atttgcgcaa gcggacgtgc aacagtcaga agaacgattg gatcagcgac
     9181 catcaggaaa actaatccaa cacacattag taacaaaaga aaatacggaa gaaatccaaa
     9241 aaaaatttgc agaatacttg atgctggaag ctaaaaaaag aagaataaaa tcaatttttg
     9301 cccagtttta atcaaaaaca gtaatagaag gagaaataca atgagttgtt taggaaatgt
     9361 catctggttt atattcggcg gactaatcgg tggtcttagc tggttagcag cggggatact
     9421 ttggtgtatc acgatcatag gtatcccaat cggtatgcaa tgttttaaga tcgcctccct
     9481 ttcttttgcg ccttttggca aagaagtaat ttatcaccag tctggcatgt cattgatcgt
     9541 taatatcatc tggctgatcg tttcaggcct accaattgct ctcgcacatg tcgtcagcgg
     9601 catccttcta tgtatcacga tcatagggat accttttgcc aagcaatctt tcaaacttgc
     9661 tcgattggcg ttgatgccat tcggtgcaga aattgtataa aagagaataa taaaggttag
     9721 ataaagcata gagccaggtg ggatacatgt atttcacctg actctatgcg ttttcctgta
     9781 taagttcttc tatgtgactt ttacgtcttt tatttatatg gggttactgt ttatggaaag
     9841 ttgaaataat tttcattaaa gaactctttc tatatttttg aaagcgtagt ggagcttcgt
     9901 atatctggga aggacatttt gcagggattg aaaaaagata ttgtcctatc gaaaaattaa
     9961 tgaatttaga caaattgcca gcaacaggct tcaaacttag ttgctatcct gtcaagatca
    10021 agaaggcaag tgcgggttgg ataagagcag tggcaatgat cgaagagaaa aagaaagaat
    10081 aagccaaaac atctgtatgg aagaaatttt ccgatgcaga tgtttttcat tttttctttc
    10141 ggataccttg acatatgaat aaccattcat ttataattag ttatgaagag atattcatat
    10201 gttgaggcga ggaggtcatt aaaatgaaaa gttacagatt agaaggttta gattgtgcga
    10261 actgtgcaat gaaaatcgaa aaaggagttc agaaaatcaa tggagtcaag gaagcaacgg
    10321 taaacttcac ttctggaaaa ctaacgattg acgcagagga agatcatttg gctacgattg
    10381 aacaagaaac aaaaaaagta gtgaaggaat tagaaccaga tgtcaaagta acagaaatag
    10441 ataaggaaaa agtatctgaa catggaaatg aaaaaaagag aaatacgctg ttccggattt
    10501 tattttccct tgcaggtatc gctttattgc tattatttga ttttaatgaa ccaattcgct
    10561 tgatagggta tttattgatt tatctgctga taggatatga tgtagtgaag aaggcagtga
    10621 tgaatatcgt caaaggaaaa atctttgatg agaattttct tatgtctgtt gctacgattg
    10681 gcgctatgtt gatcggtgaa tatccagaag ccgtagcagt gatgctattt tatcaaatcg
    10741 gcgagtattt ccaaggattg gcagtcagtc attcaagaaa atcgattcgc gaattgatgg
    10801 cgatccgtcc agaaacagct cacgtgcaga cggcggaagg attgatgacc gtgaaccctg
    10861 aagacgtgct gatcggtcag tttgtacttg tgaagcctgg tgaacgtgta ccgttagatg
    10921 gaacgatcat cgaaggagaa tcattagtcg atacgtcagc acttacagga gaatcagtcc
    10981 ctaaatctgt atatgttggt gaaacagtat taagcggatt tatcaataaa aataaaccat
    11041 ttttagttca agtagaaaaa tcttatgaaa attctactat cagcaaatta ttagaattag
    11101 tagaaaatgc cagcagtaaa aaagcaccag ctgaaaattt cattactaaa ttcgcgcgct
    11161 attacacacc agtcgttgta ggattggccg ttttattagc tgttttacct ccacttgttg
    11221 tttctagagc ggcgttttct gaatggatct atcgagcatt gacattttta gtgatctcat
    11281 gtccatgtgc attagtgatt tccgtgccac ttagtttctt tggcggtatc ggaggagcaa
    11341 gtaaaatcgg tgtattagta aaaggttcaa attacttgga gttgcttgca caaactgaga
    11401 cagtggtatt cgataaaact ggcacattaa cgaaaggtga cttttcgatc cagacgatcg
    11461 atccaacagg aattgatcca aagaaattta tgcaatatgt tgcaagtgca gaacagtttt
    11521 ctacacatcc gattgctcaa tctgttttag aaggctacgc aggtccgctt ttaccaacag
    11581 ctaatatcca ggaatttgca ggtgaaggga tcttagctga agttgatggc aaacaagtat
    11641 tagtaggtaa tcacaaattg atggaaagat ttgaaatctc ttttccttca agtcaagaaa
    11701 tcggtacatt gctttatcta gcgatcgatc aaagttacag tggttatctt gttattgcag
    11761 ataccttgaa agaagatgcc gtagacgcat tggttcaatt aaaacaggct ggtgtgaaaa
    11821 acacagtcat gttgacgggc gactcgaaaa aaattgctga tcatatcggg aagcaagtag
    11881 gcgtggacaa gatatatagc gaattgcttc cagaagataa agtgcagcgg ttagaggaaa
    11941 tcttacaaag taacaataaa aagaccgcat ttgtcggaga tggaatcaac gatgcaccgg
    12001 tgctagcaag agcagatgta ggaatcgcaa tgggtggatt gggaagcgat gctgcaattg
    12061 aagcagcaga tgtagtcatc atgaatgatc aaccatcgaa gattgcagaa gcaatccatt
    12121 tagcaaagaa aacattgaaa atcgtcaaac agaacattgt ctttgctatt gggataaaaa
    12181 ttttggtact ttccttaggt gcttttggtt ttgcatcaat gggagatgca gtatttgcag
    12241 atgttggggt gacagtatta gctgttttga atgccatgcg aagcttgcat gtaaaaaata
    12301 aacgttgatc aatgaagagg acgaatgaac gtcctctttt ttttatatcc ttttcttaat
    12361 gaaaaatgta aagaaaatag ataggattat aaaataacta atgtttttaa ttttgcagaa
    12421 aattcaaaat agcttgaaat tcaataaggt caatgaaata aaagaaaata acttattttt
    12481 ttgatggaaa aaatatgaaa aaacgatgtg tattaaataa aagaatacac acataaaata
    12541 acactgtctt gtaagtttgt taaaaattga atatgattac atctggataa caaattttta
    12601 tcataaagga gagaacgaat gaacaacaaa caattacgct ggtttactgt tgcgttaatt
    12661 gcatttaaca tggtttgggg catggggaat gtagttaata actatgctca gcaaggagta
    12721 tcagttgtca cctcttggct attgattttg gccatttatt ttattccata cgcattgatc
    12781 gttggacaat taggatcagc cttcaaggat agtaaaggag gcgtcagttc atgggtcgaa
    12841 aatacgacat ctaataaacg actggcatac tatgccgctt ggacttactg ggtcgtccat
    12901 attccttatt tggcacaaaa gcctcaagca attctgattg ctggtggttg ggcagtccaa
    12961 ggaaatggaa atatcgtaaa tacgatgtca gtacaaatgg tagcagttat ttcacttatt
    13021 atatttcttg cctttttata tttatcaaca aaaggacttt ctactttgaa agtgattggc
    13081 ggactggccg gaacagcgat gttcgtcatg tcgatcttat ttattctttt agctgtaacg
    13141 gcaccgttca tcaatccaac gatggaattt gccacaccac atatgaatca agtgaaaaca
    13201 tatattccaa catttgattt cagttatttc acaaccgttt cgatgctagt atttgcagtt
    13261 ggaggggcgg aaaaaatatc cccttatgtc aactctacaa gaaatcctgc aaaggaattt
    13321 cctaaaggga tgatcttctt agcagcaatg gtagggattt gtgctgtatt aggctcagtc
    13381 gcaatgggta tgctgtttgc tagcgataat atccctaaag acttgatggc aaatggggca
    13441 tacagtgctt ttcaaatttt aggtaattac tatggtatcg gtaactcatt gatgatcatt
    13501 tacgcattga caaacatgat tggacaaatc tccgcattag ccttttcaat tgatgcacca
    13561 ctaaaaatct tgttagcaga tgcggatcca gaatttgtac caaactggct acgtaaacgt
    13621 aatcgtaaag gtacattggt gaatggttat cttctgacag gtatactagt aggaatcatt
    13681 attttgttgc cgatctttgg tatcaaagat atgaatgaat tagtaaaatg gttgacgaac
    13741 ttgaactcag ttgtcatgcc aatgcgttac ctatgggtgt tcttggcata tatgatgttg
    13801 aataaagctt ataagaaatt caattctgaa tataaatttg ttaaaaatcc aaaaatcggt
    13861 tttatctttg gtgcttggtg ctttgccttc actgcgtttg catgtatcct aggaatggtg
    13921 ccaaaagtag attatactgc tgatcctaaa gcatggtggt tccaattatt cacgaatatc
    13981 atcacaccaa ttgtcttgat cttattaggt atgatcttgc cagcaattgc tcgtcgagac
    14041 aaaaacaaag aaattactga accagaaaca gtggttcagc cttaaaatag aaatacttgt
    14101 tacaagaggt tgtgacagaa gctacttctg gaacaccgtg aatcagaggt tgtgaaaaag
    14161 ctgtcctgca tcaagtaata agaacagcca aacaaaaata gctcctcata ttttgccgtt
    14221 ttttgagcta atgctgaagg tcagctgatt gaacaccatt tattcgaaac aagggacttt
    14281 gacacgactt ttgtcacagt cccttgtttt
//
LOCUS       DAZKOU010000073        13980 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000073 DAZKOU010000000
VERSION     DAZKOU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.074"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..1005
                     /locus_tag="ACG827_002130"
     CDS             142..1005
                     /locus_tag="ACG827_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0484254.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1134..2756)
                     /locus_tag="ACG827_002131"
     CDS             complement(1134..2756)
                     /locus_tag="ACG827_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0484255.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3005..3526
                     /locus_tag="ACG827_002132"
     CDS             3005..3526
                     /locus_tag="ACG827_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0484256.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3712..5442)
                     /gene="spxB"
                     /locus_tag="ACG827_002133"
     CDS             complement(3712..5442)
                     /gene="spxB"
                     /locus_tag="ACG827_002133"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0484257.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5607..6425
                     /locus_tag="ACG827_002134"
     CDS             5607..6425
                     /locus_tag="ACG827_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0484258.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6528..6725)
                     /locus_tag="ACG827_002135"
     CDS             complement(6528..6725)
                     /locus_tag="ACG827_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484259.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6894..7472
                     /locus_tag="ACG827_002136"
     CDS             6894..7472
                     /locus_tag="ACG827_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0484260.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7567..7704
                     /locus_tag="ACG827_002137"
     CDS             7567..7704
                     /locus_tag="ACG827_002137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484261.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8517..9020)
                     /locus_tag="ACG827_002138"
     CDS             complement(8517..9020)
                     /locus_tag="ACG827_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0484262.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9379..10404
                     /locus_tag="ACG827_002139"
     CDS             9379..10404
                     /locus_tag="ACG827_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0484263.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10727..11605
                     /locus_tag="ACG827_002140"
     CDS             10727..11605
                     /locus_tag="ACG827_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0484264.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12081..12197
                     /locus_tag="ACG827_002141"
     CDS             12081..12197
                     /locus_tag="ACG827_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0484265.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13125..13463
                     /locus_tag="ACG827_002142"
     CDS             13125..13463
                     /locus_tag="ACG827_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484266.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13557..13787
                     /locus_tag="ACG827_002143"
     CDS             13557..13787
                     /locus_tag="ACG827_002143"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0484267.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            13801..>13980
                     /locus_tag="ACG827_002144"
     CDS             13801..>13980
                     /locus_tag="ACG827_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484268.1"
                     /translation="MKNFDTYYVIVFSISLLLILLTLMQYKEYPEYFILTFVYLVSSY
                     TLLYKFRSSSRVSKVC"
BASE COUNT         4443 a         2310 c         2676 g         4551 t
ORIGIN      
        1 ttcttgaagc tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa
       61 accggttaaa gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt
      121 aacgagaagg ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca
      181 taccatgtat tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat
      241 caagagttag tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg
      301 aaaaatggtg cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc
      361 cgttcttatg cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc
      421 ggctcttaca gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct
      481 gagatctatg tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa
      541 gctgtaagat tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt
      601 aattatttga aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc
      661 ggtaggattt ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt
      721 acggtggata aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg
      781 atccagacaa tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt
      841 tatgtggctt acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag
      901 cagataaaga tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca
      961 ggctatggct gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt
     1021 ctgtaaaaaa gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag
     1081 catcctatct cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc
     1141 cataactcgt tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta
     1201 tcagctcgat cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg
     1261 ataaattgat ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca
     1321 ttcaatgctg tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg
     1381 ttagctttcg aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa
     1441 acattcactg gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt
     1501 aagaaggtgt tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt
     1561 agatcagtat cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca
     1621 ccccaacgta ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt
     1681 gttgttgtta tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa
     1741 gatatatcat ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat
     1801 aagtcgtttc caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa
     1861 ggttggatat cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt
     1921 gattttgatg cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct
     1981 tcttttttgg catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa
     2041 aaatcataat ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta
     2101 catactttgt tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg
     2161 atcaaaaatt cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct
     2221 agcaaaagaa catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt
     2281 ccaccagtta attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc
     2341 agtaaaacag ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct
     2401 aattcagctg ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct
     2461 ttttctttca gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta
     2521 tagtcttcat agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata
     2581 gtaacggaac ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta
     2641 cctgcaccat ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca
     2701 tcatcaaata gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt
     2761 tttcctcact tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg
     2821 taaaagattc aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat
     2881 aaaaataaaa gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat
     2941 ttttgacgtt atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt
     3001 gaaaatggat gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt
     3061 acttgtctca ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt
     3121 ctttgcgatt tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa
     3181 agagctaaca ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt
     3241 tggcgtcttt ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc
     3301 agtatggttt attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt
     3361 aagtgaagga tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat
     3421 tttcttattg tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta
     3481 ttttatgtta tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa
     3541 ataaaaaagc cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat
     3601 catggacaaa tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg
     3661 agaagctata tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct
     3721 cttttcatat aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc
     3781 ttggatttct tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg
     3841 gcgcttgtta ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc
     3901 aaaggcaaat cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc
     3961 tccaacttta ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc
     4021 ttcttgttct gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg
     4081 aagctgatat ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc
     4141 acttaaggta aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac
     4201 accattaccc atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt
     4261 caataaacga atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc
     4321 ttcagcaata cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg
     4381 atcttcaaaa gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta
     4441 ccagctatca gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc
     4501 atcaccgagg atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc
     4561 gatttggata aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc
     4621 gacaaataag atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg
     4681 tccagcaaaa ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga
     4741 agcgacaaca ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg
     4801 cgttccttga ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc
     4861 tagcaaatct ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa
     4921 aggttgttcg tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc
     4981 tttatgctca taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt
     5041 catgactgta cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg
     5101 gaaagagtta tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac
     5161 atggtccatc tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga
     5221 gccgaagaca gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc
     5281 gacttcttca tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc
     5341 cataattgaa ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc
     5401 aagaactttt accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag
     5461 caatgatgat acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa
     5521 ctaattcgtc ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat
     5581 agatagattc aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc
     5641 agcaagcact tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg
     5701 tttttttctt taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg
     5761 gtgtcatgat tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag
     5821 aaaatggaga tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt
     5881 tatatatggc agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag
     5941 gattgcctac ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca
     6001 ttcctgaagg tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga
     6061 cagccgctat attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag
     6121 aaggagcagc tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg
     6181 tttacggaca agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag
     6241 taacgaaagt ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt
     6301 atgtcgtggt ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt
     6361 ttgcagtttt tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag
     6421 gataaataaa aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc
     6481 tattttttta gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt
     6541 tttctatgtg tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt
     6601 agaactttga ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg
     6661 aagctagctg caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga
     6721 cgcatacaaa accctcctct tgttcattct ttactataga aaaagtataa cagggattgt
     6781 ttaaaaacac ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat
     6841 tgctttttct ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta
     6901 aaaatcgtct tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt
     6961 tagaacttca tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat
     7021 tttttatcta tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt
     7081 tatttcaagt tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt
     7141 ttgcgctttc gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg
     7201 ctccagaaat gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg
     7261 caaaagaact tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata
     7321 attacaagaa atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt
     7381 tagtagcgcc agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc
     7441 caagtcgtac catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa
     7501 aaatatagaa aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg
     7561 atgcagatgg atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt
     7621 atttatttgg atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca
     7681 ttattcacag caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag
     7741 aaaagcatgt ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt
     7801 aatttttaga aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg
     7861 taagagataa aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga
     7921 tatacaacag aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt
     7981 ttggaataaa aaaataacat atctcatttt acacttttgt tattttatac ttaaaaaata
     8041 acgacacaga aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt
     8101 tcctttgaaa tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga
     8161 agggagccta aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag
     8221 ctttttatag aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta
     8281 gattgagata ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc
     8341 ctttttgtta aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc
     8401 aataatatag tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag
     8461 accgtcatag atacacattg gaatgtgtct attacggtct ttttttacga acgattttat
     8521 tgaagacgga cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg
     8581 tatcccaaaa taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt
     8641 tgataggccc ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc
     8701 gctacgccaa taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata
     8761 atccctaatt ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa
     8821 ggaaaaaaga caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa
     8881 aaagtagaaa aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga
     8941 ttaaatgata gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa
     9001 aattttgata cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg
     9061 ctgaaaaatc ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta
     9121 gaagcttttt tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg
     9181 gaaaagttgc tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt
     9241 tctccaagtt gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata
     9301 ctaggtaatg ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta
     9361 ctaggaagga gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat
     9421 ccttttcgta tcatgaatct gatgctactt atctttattg tattatggtt aggttacata
     9481 aatcatgatt ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat
     9541 caaaacatac cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg
     9601 gttgctttcg ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta
     9661 gctttggttg ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt
     9721 ggcttgtttt ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa
     9781 ttaccgatcg tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg
     9841 attacaaaat tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa
     9901 agatttcacg aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt
     9961 ctttccgttt atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca
    10021 tgtgcatcga ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat
    10081 ctgattggat cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg
    10141 cagttgcaga ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct
    10201 agaaattatg cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt
    10261 attcgtggag catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga
    10321 agtttcatcg gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg
    10381 gttgttcgtc aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa
    10441 gatttttgtc acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca
    10501 aattcacaca aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa
    10561 aattttgttt atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa
    10621 agtcttacag atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa
    10681 agtatgttta taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga
    10741 tatgaaaagg ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac
    10801 tttaactctt cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga
    10861 tgaggtgttt gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc
    10921 aaagaaaata gacaaagtct atgaagagga accttttgca gcattagcta tttatctata
    10981 tgcagcttct caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta
    11041 tttggcaaca atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag
    11101 ctatcgcttt gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat
    11161 tggtgaattg gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga
    11221 aggaaagcag tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat
    11281 aagagatagt tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta
    11341 ccaagtgatg tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa
    11401 agagtcaaaa atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga
    11461 cgatagtgga gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga
    11521 aagtacgagt ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc
    11581 tggcttaggt attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag
    11641 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    11701 gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt
    11761 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga
    11821 acacacagta cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca
    11881 agagggcctt ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag
    11941 tcaatcagct agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac
    12001 catttaaaac acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa
    12061 gaagaaagtg ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga
    12121 aaagcccaaa tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga
    12181 atagtgggaa aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt
    12241 cggctatttt caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc
    12301 catttttaac atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa
    12361 ttccagttat atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct
    12421 gtagttattt ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta
    12481 ccttttaaca cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac
    12541 ataatcaaag gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg
    12601 ttgcaaataa ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt
    12661 cagctcactc catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc
    12721 gaaaaagtta actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt
    12781 actattgcta gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa
    12841 gttgtgtatt ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat
    12901 cactattaca agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta
    12961 aatgaatagt tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt
    13021 tcaatttgtg attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga
    13081 ttgtagtgtt tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt
    13141 caagtgagga acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa
    13201 atattgttcc ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa
    13261 taggatggta tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata
    13321 cagtaggctt agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata
    13381 ctgatgaaag tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata
    13441 aaaagtatat attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga
    13501 aaatttacta gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc
    13561 tggtagaaaa gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg
    13621 aaaatgagtg gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag
    13681 tttacagaga aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt
    13741 tagctgaagt agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt
    13801 atgaaaaatt ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta
    13861 ctaactttaa tgcagtataa agagtatcca gagtatttta ttttaacttt tgtatattta
    13921 gtaagtagct acacacttct atataaattc agaagttctt ctagagtttc taaagtttgt
//
LOCUS       DAZKOU010000074        13944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000074 DAZKOU010000000
VERSION     DAZKOU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.075"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..219)
                     /locus_tag="ACG827_002145"
     CDS             complement(<1..219)
                     /locus_tag="ACG827_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0484269.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKF"
     gene            complement(698..1417)
                     /locus_tag="ACG827_002146"
     CDS             complement(698..1417)
                     /locus_tag="ACG827_002146"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0484270.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1466..3112)
                     /locus_tag="ACG827_002147"
     CDS             complement(1466..3112)
                     /locus_tag="ACG827_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0484271.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3291..3881
                     /locus_tag="ACG827_002148"
     CDS             3291..3881
                     /locus_tag="ACG827_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0484272.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3973..5478
                     /locus_tag="ACG827_002149"
     CDS             3973..5478
                     /locus_tag="ACG827_002149"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0484273.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5562..6914)
                     /gene="celB"
                     /locus_tag="ACG827_002150"
     CDS             complement(5562..6914)
                     /gene="celB"
                     /locus_tag="ACG827_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0484274.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6914..7432)
                     /locus_tag="ACG827_002151"
     CDS             complement(6914..7432)
                     /locus_tag="ACG827_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484275.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7448..7774)
                     /locus_tag="ACG827_002152"
     CDS             complement(7448..7774)
                     /locus_tag="ACG827_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0484276.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7787..9766)
                     /locus_tag="ACG827_002153"
     CDS             complement(7787..9766)
                     /locus_tag="ACG827_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0484277.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9787..10101)
                     /locus_tag="ACG827_002154"
     CDS             complement(9787..10101)
                     /locus_tag="ACG827_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0484278.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10269..10562
                     /locus_tag="ACG827_002155"
     CDS             10269..10562
                     /locus_tag="ACG827_002155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0484279.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10640..11752)
                     /locus_tag="ACG827_002156"
     CDS             complement(10640..11752)
                     /locus_tag="ACG827_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0484280.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11905..12753
                     /locus_tag="ACG827_002157"
                     /pseudo
     CDS             11905..12753
                     /locus_tag="ACG827_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13106..>13944)
                     /locus_tag="ACG827_002158"
     CDS             complement(13106..>13944)
                     /locus_tag="ACG827_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0484281.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT         4350 a         2852 c         2386 g         4356 t
ORIGIN      
        1 aaatttgaaa ttttcccaac ccacccaagg actttccctc aagctagcca agaacccatc
       61 tgctgaaata tgaaaatttt tgaatgctac gacattcgcc attacaggta tgtaaaagaa
      121 aaaaatcatc caaagcatac ccggtacagc catcaggaca agtgctttat aacgccatag
      181 attcataaaa aaatgattga acccctttcg ttttttcatc taaaaacccc tttcgattca
      241 ttcgcaacgt aattatagcg ctttcaataa tgagaataaa taaacaaagt atagggaaaa
      301 tgaaacaaag tataggagag gttgtgaaat cagcgttttg gcctgagtat tagagaaaaa
      361 aacgggaaaa tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga
      421 ttgaacaccg tttatcagag gttgtgaatt cagcgttttg gcctgagtat tagggagatc
      481 gtgtccttac tatctaactc tgagtcacaa ggaacaatgg ggactgtaac aaaagtcttg
      541 tcacagtccc ttattccgaa caaacggtgt tccagaagta gcttctacgg aaataagccg
      601 aaatttcaca aaaaagccat ccgaaagtca gacggacaag tctgcttttc agatggcgct
      661 acaactactc tgcttattct tttagctatc cattcaatta tttttctctc acttgttcta
      721 gctcttcttt ggtcatgggt cgatattgcc ctttattcaa atctttaggc agccaaaatt
      781 ttcccatacg gattcttttt aaatataaga cttcttttcc tactgctttg accattcgct
      841 taacttggtg aaactttcct tcatgcaaaa tcagacggat aattgaagtt tctgtctctt
      901 gatctacttg atcaatttgg agttttgccg gcaatgtctg ctcatcgcca tcaataatca
      961 gtccttctgc aaataaacgg atatcttctt ctgtcattat accgctaacc tcagctagat
     1021 attctttctc cacatgtttt ttaggtgata gtaactgatg ggcaaacacc ccgtcattcg
     1081 ttaaaatcaa gaggccctct gtgtctttat ctaatcggcc gactggaaaa agatcttccc
     1141 gataatcctg gtcttccaat aaatcgatta cggtttgatc atgattatcc tgagtagcag
     1201 aaatgactcc tgcaggttta tgcaaaatgt agtaaaaatc tttttgatac gtaatgattt
     1261 ctcctaaata agtaaccgca tcttcgaatt catttacttg cgctttatcg cttttcacca
     1321 cttgtccatt gacttcgatc gttccttttt tgattaatcc tttgacttct ttgcggctgc
     1381 caagtcccat atctgctaaa aacttatcta aacgcatgcg tttcccccat ttcaaaagag
     1441 catcaatcta gctcttctga ttcacttatt ttatttttaa tttatgtcgt aaacgcgcta
     1501 ttcctggtcc tagtagtctt tctgccaagc ggattttcaa agctaaataa ccatacacta
     1561 aagcccctat acctgctact acaagaatca acaatagaga ctggaatttg ctatcttcgt
     1621 ttaaaaaagc accaaaaatc atctttgtga tccacgaagc caaaagcata atcaaggtaa
     1681 gaataaagat cagcaatgtc ctgcgccaga caaaacttgg tttaaagcgg gctacttcat
     1741 ggattctctt taagatcaag gtacaagaca cagcaaatcc gatcattgtc gccaataaag
     1801 ggccgtatac ttcaaataaa cgaatggctg ggtattgaag aaggagtttc actagaaacc
     1861 caataaccaa ataattaatt gccgccttgt tttcaaacat tccttgcagc atgttggaaa
     1921 ctagcatata tagtcctagg aagagtccta caaaacttgc ttggatcagt acattacttc
     1981 ctagagaatc cggtgtataa aataatgtat ttagcggata ggcaagtaac ataacaccaa
     2041 atgtcgccgg aaacataaaa aatgaaaaca actgtaaatt tccgcttgtc aattttgcca
     2101 gacctactcg atctttcaac gtaacagctt ctgtaatcaa cggtaaccca gtagacgcga
     2161 tcgatgttgc caatgcaata actaccatcg tcaacttatc tggattggca ctaaaaatcg
     2221 aaaaaagatc cagtaactgt gcattggaat actcggtgga tgcagacatg atcttcataa
     2281 aagtgaactg atcgactaat ttgaagatcg ttacccctga acccacaata ataaacggaa
     2341 tcgcttcttt aacggtatct aacaacaatt ccttcgttgc aatatttatt ttgttttcgc
     2401 tgtagtgaat aaacgccgat gtatacgctt gatgtttctt caaaaaatac aaaaggacaa
     2461 gaatacttgc tagcataccg ataaatgccg cgaaagttga ttgcgtgacc gccgtcacat
     2521 agtcaccatc catgactttc atgatgatga atgtagccaa tagcatataa aaaatacgtg
     2581 ccacctgttc tacaatctgc gacagcgcat aaggcatcat ttcttgattg ccttgcaaat
     2641 accctcgtat cacactcata caaggaaaaa ccaatacagc gatactcaaa gaacgcatcg
     2701 ttggaatcag ttcttctccg ccaccagaag catgagctag ccaaggagag gcgatgtaca
     2761 tgaacaaagc aaacaacagg cctaacccgc ccatcatctg taatgcacgg ataaataatc
     2821 gtcgtgaagt gccatattca ttcaatgaat tatagcgtgc agtttgtttt gcaatcgcag
     2881 caggtattcc tgctgtagag atcagtagaa acaatgcata tatattgtat cccatattga
     2941 ataatccatt ggcagcctta gcattttcgc ccatccaagc ataccaggga atgatataga
     3001 cagcgcctag caatcttgaa atgatattac tggcggtcat ccaagcagag cctcgtgcca
     3061 ttttttcttg cggtgttaat tgatctaatg ctggttttct ttgattatcc atcggtcaga
     3121 ttccaacttt ctacaaaaca ttactatcat tttaattggt aaaacagaaa ctggcaatcg
     3181 attttatata acttttataa gaacaattgt ttttttgaat tcggtttcgt ttttttggta
     3241 aagtaaagaa atatacagcc ttttatggct aacatttata ggagatatca atgacacaag
     3301 aaataaaaaa actacccgtt atatttttag gtcttttctt tatcacagtt ggattgaatt
     3361 ggttcttgct ccctcacgat atagcttccg ccggagtggg cgccatcgga catttagttg
     3421 aaacggcctt ttctatccaa cgctcagtta ctgtttggag catcaatctt atgatgctgc
     3481 ttctagccgc tgtcctatta ggaaaatcag tattttttaa gactgtagcg ggaagtctgc
     3541 tttttcctgt tgttttagag attgttccaa aagtggaaat gatctcctca catttctttt
     3601 cactgatttc aggaagtctt ttgttcagtc ttggggttta cacattgtac acagtcgggg
     3661 cctcaaatgg cggtataacg ataccgccga ttattttcca caaatttttt cggcttccaa
     3721 tgccgcaagg acttttactt accaatagtc ttatcgtctt tttgaattat tatgtttttg
     3781 gattgttgga gacactattc gttctcctat ctattacagt cagttcattg tttatgaaaa
     3841 tactcatccg cttgtcccct gtgtcaagaa aatttgaata aagctggatt ttgtcatagc
     3901 ggaaacaaac gatatattaa aaagagtgtg ctataataaa ccaagaaatt gaataaagcg
     3961 agggatcaat caatgagctt ctctttagaa gacatccgtc atttactatt aaaagaaaat
     4021 ctattaaaag aatttgtttc aacaagaggc tggcatcttg atgtaccaga aaaagctgaa
     4081 tttcttcagt taagttatga ttcaagaaaa gctgatgctt ctactttgtt tttctgtaaa
     4141 ggaatgaatt tcaaagaaga atatttggat tcagcaatcg aacagggtat tcaatattat
     4201 gtatcagaac aaccatacga aaattcagca gtaggaatca tcgtaacgga tatccgtaaa
     4261 gcaatggcgc ttcttgctat ggctttttac gactatccgc aaaataaatt gaaagtgatc
     4321 ggattcactg gaacaaaagg taaaacaact gctgcgtact ttacaaaagc aatcttggat
     4381 cacacaacaa ataaaaaaac agcattgctt tctactatga atacaacgtt agatggcaaa
     4441 acatatttca aatcccattt gacaacgcct gagtcattag atttatatcg tatgatgtca
     4501 gaagcagttg aaaatggtat gactcatcta gtgatggaag tttcttcaca agcttacaaa
     4561 acacaacgcg tatacggctt gactttagat gtcggaatct tcttgaatat ctcgcctgac
     4621 catatcagtc cgatcgaaca tccgacattt gatgactatt tctattgcaa gcgacaattg
     4681 atcttgaatt caaaaacagt tgttttaaat catgaaagtg actattttga tttacttagg
     4741 gaaacagccg atctattcca gattccaacg atcacttatg gtcgttcgga agaaagcaat
     4801 tatcaagtga tccgttcaga taaaggtact cacggtttta ctttatcttc tcatgaagat
     4861 cgcttagcta tctgcgatac tgcttatgat attttgctgg ctggaggatt caaccaagaa
     4921 aatgcagcca gtgcgatcat tgccgcagca ctaacaggtg cctccagtca agatgcacaa
     4981 gacggattaa aagaagctcg tgtaccaggc cgaatggatc aattgatcca atccaatgga
     5041 gcgcatgtct atgtggacta tgcacataat tatctaagct tgaaaacatt gctgaaattt
     5101 gccaaaaatg aacatccaga tggccgtgta attgttgtat taggcagccc aggaaacaaa
     5161 gcaatctccc gtcgacatga ttttggaaaa gtgctatcag aaacagctga catcgccttt
     5221 ttgacggcag atgacccagc atttgaagat ccgaaaaaaa tcgcggaaga aatcaatgaa
     5281 gcaattacta atccagattt gatcgttcat tatgaaatgg atcgtcctga agcgattcga
     5341 cgcgctttag cagaaagcgc tccgcaagat tcagtcgtta ttgcgggaaa aggcgtggat
     5401 ccctatcaaa aaatcaatgg tgtagatgaa ccttatgaag gggattatgc aatcgtgaag
     5461 cggttgattg aagaataatg gtatttatat actagtggtt tcccatttct aagaaaaaaa
     5521 gggactgtga ttttttgtca cagttccttt tttcttagaa atcattttaa cttttcacta
     5581 gctaattctg ctgccttttc tctttcttca tttaataatt tattatcata gaatttgata
     5641 aatgggaacc agataatgaa tgcgatgatt gcacaaatga ctgctaacac agcagctttc
     5701 caatcaccac cactaccaac aaatgctccg atcccgactg gtgaaggcca cggcatctgt
     5761 gcaagcatcg gtttgacgat ttccagccga atggcaaaat aagcgagtgt agcagaagcc
     5821 atcggagcta agaaaaacgg aaccgctaaa tagggattgt agacgattgg catcccgaaa
     5881 atgattggtt cattgatatt gaataatcct ggtacaagtg aggcttttcc taacactttc
     5941 agctgatcag atttcgcgag gaatgcaata aagatgatca atcctaaagt cgcacctgat
     6001 ccaccaacgg ttacataaga attgttgaat tctcctgcca acggataatt tgcgccttgt
     6061 gcgttagctg ccatattagc taatacgatc ggggtcaaga aagaagtgat gatattcgct
     6121 ccatggatcc ctacgatcca caatgcatgg atcaagaaat aaatcaccat gacaccaagc
     6181 catgtattcg tcaactgcgt aacaaaccca aatgggatag caatcatttt gaagatatcc
     6241 gttcctagtg caactaataa tccattgatg atcatcacca caaacgcaat gacaaatgtt
     6301 gggatcagag cagtgaaaga acgagaaact cctggcggaa caacatctgg catttttatg
     6361 acccaatttc tcttgacaca catacaatac aattcgaccg caataatcga cataataata
     6421 gctgtaaaga ttcctgaagt tcctaaacgt tcgacaaaag agcccatcct aataccattt
     6481 actacagttt ctgtctcatt gatagaagca atcaagctga ttttaccatc agtcatgatc
     6541 aattcaggga tacacataaa gaatgcaaaa acagatagta atgcccccgt gagcggatta
     6601 acatttaatt tttgttcatc tcgttcaatt gacgttaatt cataagcaaa cacaatggca
     6661 aagtaaatag ataaaatccc catcgtcatt gtattgacga tcatgtacaa gtcactaact
     6721 ttgaaaaaag ttgcattaaa aaatccttct aaaaatggga acggtaatgg caaaatattt
     6781 aaaaccaaaa acatcgagcc tacaatcgtg aaaggaatag aagccatccc cgctgccatg
     6841 acagcccgaa cgattttaag ttgcgcaatt ttccccatag gtcccatcag gtatttctgc
     6901 aaaaaatcaa acattagtta ttctcctttc ctaaatataa cgctttttca tattgtttaa
     6961 tacggatata ctgttggtct tggttgagtg aaattttctt taaacgatac tgtataaatg
     7021 cactggcaaa aataccgctt acaacaagaa tgaaaacaaa taactgtcct ccattgttat
     7081 ttgccataaa aggatacaag aatgtaaatc ctgaggtaaa caacactgga atcactaata
     7141 aattaaagac actctggaac aaaaaatacc actttgtcca tggaacgttc gtttggtgtt
     7201 ttcgataaag ctttacttgt tctcctactg caggcaaaat agctaaaatc agaatcatcg
     7261 gtaaactact tcctaaactt ttagctgata gaagcagaat catccattgc atgttcatga
     7321 aaaagaaaaa ggcagtcagg taacgtacga ttagataacg attgaaatac atgtttttca
     7381 aactaagttg ctttttttct aattcgattt tcctttttac tgtatcctgc acaatgatcc
     7441 ttcctttcta tttatttttt tgatacaaat aattcatttc ctgtgcaact tccaataatg
     7501 tcatcgtcgt catcagatga tcttgtgcat ggaccatgat aatttccatt tctatttttg
     7561 tcccgctggc atattctttc agaagttgag tttgtgactg atgcgcatga agaatctctt
     7621 catttgcttg tcgcaacttc tcttctactt cttcaaacat ctctttacgc atcgagcgaa
     7681 atgcttcatg tatcagtgtc cgtgcatttc cgctatggaa aatgatatca aaagaagtca
     7741 cttgtagtgt ttcactgttt attttttcag ccatctgttt tcctccttat tcgatcagtt
     7801 tgataaattt actgctgaaa ttctcaaaaa taggttcagc taagatttcc tgctgtactt
     7861 ttagctggtc aactaaccta acgattgctt tagtcacgac cgtaattcct tcattttcga
     7921 cataagaagg cgaaactaaa aaaacaaaat gaatttcttg atattgatcc cactgcatcc
     7981 cagaaggaat caatgcaacg gctacctttg tttcgattcc aactggaaca gcgggatgtg
     8041 ggactgccac tgtatcactg aatatgattt gtcccatatt ttcacgttgc tggatctgct
     8101 ttcgcatttc tcttatatat ccttcttctt catttatgct cagcttttgc agcaaatcat
     8161 gaagaatttc ttcctttttc ggttcgtacg tataagtctt gaaaaatttt tcagacaact
     8221 gttcggtgta aatctgcttt ttttgctgta gagaaaatgc tggtatcggt ctatctgagg
     8281 aatgataggg tatagattct ccaactacct ttcgtatttt acgaacatca tcttcattca
     8341 aaaaaatact gacatgtaaa actggaattt tgaaaaacat tgtcgaaaga tcaatagaag
     8401 aaataatcag gtcgattcct tttaaagttg tttcattgat ctcataatag ccttttacac
     8461 ttaccactgt aatattttta ccgaattcac tgactacgcg attctttaat agttgggcac
     8521 tgccgtaacc cgttgcacag ataatcaatg catgtagttt atgcgcagct ctttcttttt
     8581 ccaatgccgc catgagatgc aaagccaaat aggcccattc atcttcattg atggtatagt
     8641 ttttcaaagc cggcatgttg ctgaaatgcc tttttactgc gtcaaagact ttgggatttt
     8701 ctttctttat ctcctcagtc aatgggttct caatgcgaat acctcggctc aatcgaatca
     8761 tcatcggtga caaatgatcg agtagtccat tgaaaagctg gagatcctct actaatgaaa
     8821 aatgaaattc ctgagacaat cttttcagaa ctttcgctaa ttcctttgat agttcctgat
     8881 cattttgatt ttctttgtga ttcgacttag ccatgagatg taatgtcaaa taagcaattt
     8941 cttcttcggg aaacgataca cctgcgatta cctcaatacg ttgaacgatc cgctgcgcca
     9001 cttgatactc tactcgctta gaaacttctt gttcgatgcc taattccttg atttcaaacc
     9061 cttctcggag ccgtttgatc cccaaagcca agtgtaaagt cagattctgt atgataaaat
     9121 ccgaaacttt taacttagct tctcttgttt catcgagaat gataatgact agctcttcaa
     9181 aactgatgtc tgggaaaaaa cggctttgcc ctaaatattc tttcaatgaa tttccataat
     9241 ggtttccaaa aaaagtatcc atgataaaat gccgtcgatt tctttcttcc ccttcgatcc
     9301 atgtcccttg accatgctta gaaacgattt ttagtgaata tggaatcaat cgctccttga
     9361 tttcttgcat gtcctttgtc aaactggaac gactgatata aagctcttct gccaaatcat
     9421 ccataaaaag aatggcatct tccaaaagca attggttcaa tatatacttt tgtcgatctt
     9481 cggactcata aaatccttgc ggttctctag catatccagg tactatttct tgccgactca
     9541 aaaacaagtc aaatgtcagt ctttgtaaaa tatgcagttg gtagccataa cctggtttag
     9601 caatgatctt acctccgttt ttttctacta actctttgat ccgctgcaag taattacgga
     9661 ttgtccgatc agataataac aattcagaag ctaaacgttg actggtgaca tactccttct
     9721 tgtgatcaat caaatatctc aacagcaatt tttccttctg tttcattatc atccactcct
     9781 attccatcat atgttctctt cgatcacctc tgctaacttc tggatgcctg tagggattgg
     9841 gacatacgct tggaaaggga tactaacaac aggctttcca acagattctc ctaatttttt
     9901 gaatttatcc aaaaacatcg tcgtttgcgg gctgatcaga aataaatcaa aatcactatt
     9961 cttaatcata ttatcgcctt ctgtagctga aactgcatct acgagtacct cttctctttt
    10021 ttgcttaaag aaatccgttg tctttgaggc catcatcgag gatgacattc ctgccgcaca
    10081 aataatcaat gcttttttca taaatcaatc actcctttta tttttataaa ccaagcatat
    10141 cttgtaagcg ttttctattc aaaatatata tttccgtagc attgcggaaa aaatgtgtat
    10201 catatcttga ataataattt ctggcaccgt gatatactag tgatagaatt ttttcaggag
    10261 gggatttagt gagtatcaca gtgaatattt tgtatacaag tacaaatgga caagccagaa
    10321 aatttgcaga agaaatggtc gaaagaaaca ttgtcaaagc gattcgtgca gaagaaggaa
    10381 acgagaaata tgactattac tttccactag aagatccaga atctctttta ctgatcgacc
    10441 gttggaaaga tgaagaagca atcgaaaagc atcacaaatc cgaaatgatg gctcaaataa
    10501 acgaactccg taaaaagtat aaattaaaaa tgaaagtcga acgtttcact gatttaccgt
    10561 aaaaagatac acatggaatg atagcaattt atcatccatg tgtatcttca ctttttcctt
    10621 attttttatt cgatctcatc taatgattca aaccgtgttt ttctcttatt tttcggataa
    10681 gtatttcagt caccccaata tacatgattc ctaaacatac cccaaggata ttctcaaaaa
    10741 tgcggatcgt tactgctcct ggaaccccga ataaactggc agtgattgcc aaagcaccaa
    10801 aagaattaaa aactgtttta tatctatagg tagaacaaac acctaaagct agaccaccta
    10861 atataccgac ccagttaaaa ggaataaatt ggctgatacc aataaataaa acacatccaa
    10921 tcaatgagcc aatgattcgg tctactgctc gttctttgaa accaatcgac atcaatccat
    10981 agctagatac aatagaagaa aatgcaaacc ctgcccacat caatcgctga aaagggacat
    11041 actctcctgc aacgatcaaa agactaatgc caaatgcata gatgagcatc caaatttttt
    11101 cctgcgaata catgcctttc aaaaaaatct ctttaaacaa gcttttgtct ttgttctttt
    11161 ctcgatgttt tctgtaaagg agaactgaaa accaacaaaa aaacaaaaat aataagctac
    11221 ttgtttgtgt aaagaaattc ttgttcaacg aatctttagg taacgaataa acaataaaga
    11281 gatatgaaaa gccgtacaaa gaagcattcc ccattttggg atcactagcg gttgccatta
    11341 atatgctgct tagtaaaata aaatgaatca acatgcccat aactgaccat ttgatcagct
    11401 ggacatacgg tgctactaat agtgagaaca tgacgattgc taaacctatg atcgaatggg
    11461 aaatcttata accaaagtca acaaaacgaa tactcatcaa catacagaat aaaaccacag
    11521 cgtatggttt atgcttcgat tcaaataatg ttgtaattat aatgataaaa caaatggcaa
    11581 acaatacgat gaataatgat cgtaaaaaca atgaacaaat atacatgcac ttttcttttt
    11641 tagaagctgc ttctcgtatt ttttgtttta aaataaacgg atctagttgt agagactgat
    11701 aaaaatccac ctgttgtcgt cctttctttt tacttttatt ttttacagtc atctatgaca
    11761 ggttgtaaat actatggaac atcatatggc gttgtcattt tcttgtcaac tatttttcct
    11821 attcatgttt ttataaaaaa acatatcgtt ttcttgtatg attaaatcac cacaaaataa
    11881 acaactgaga gaacgatatg ctttatgaat ggttctatca aaaaaattct gagatcaata
    11941 gacaaaaatt tgatgattac agaggtctct tacgaaacct tacagaagaa aaaaacattg
    12001 atcgttgctg cagttctctt ccctgctcca tgtgtctgta gaaattgtgg ttctgctgtg
    12061 gtagatggaa ataggaaatt aatggtggtg aaaaacaaga agaaggaaac cattgtccac
    12121 tttgaataat ataatcatat gccaatggtt atacgcctaa aaaagcagcg ctatacctgt
    12181 aaaaactgtc gaagccattg gaacgctcaa agttatttta tccgtccaag gcactcgatt
    12241 tcaaatcatg tcagacataa aatcacttct ttactgactg aaaaagtgtc gttatttttt
    12301 atttcgaaaa gttgttaggt atctttgaca accgttattc gcacattgaa agagttgaga
    12361 agctatttac caaagtcttc caaaagaatt ctgtcttgag tgttgagggt tgtgaatttc
    12421 gttctcacgt ttccgttgag gataaactga gttttatttc cgcggatggc gagacagaaa
    12481 aattaatcga gaaaatttac ctacacaaaa aattcctcga ttaacaaact atttccttag
    12541 ctgcactaat cctaaagaaa tagaattctt agttacagac atgaacgcca cctactttca
    12601 gctcaccaaa catgttctac caaatgcgaa attggtgatt gatcgattac ttggtttttc
    12661 cacgcctttc aaggaggcgt aacctttatt cacgagttgg ttgaagcttt ccgagacaaa
    12721 agacccgact tgtttttctc cttattgaca aagagccatt aaaactagag aaaaaattac
    12781 aactgatcgc tgattatttg tagaaagaaa atcaaagtat tacccaataa acttgagtca
    12841 ttaggcaaaa aacactgcga aactcaagaa tgattttcgc agtgtttttg tatatttttt
    12901 attaactagt attactacaa aggtttattt ttcacttctc aacgcagcac aaaattgtct
    12961 ttacttattt actggttggg ttcaaccttc tctactccga tactaatatt tgtttgtata
    13021 ccccttctaa aatcggtagt ttccattcaa ttgatgggta ctagttgatt ttaaaaacta
    13081 tgcttattca attttttatt ttagctcata tatttcgttt ctttctaaat tttatactga
    13141 tgaaaacaag tagcacaccc aatattgaca atataaactg gctaatgatg gtttctccag
    13201 tttttggaag atgcttattt ccactgcttg ttggattgtt tcctcttata tctgagctat
    13261 cagcaacttt tctattctct tttgttaaaa agatcgccac ttcttgttct ttttcgatct
    13321 tgaaagttac tggtgtcgca tccaattcat accccgttgg cgcttgagtt tctaccagct
    13381 gatatgctcc tggtgctaag ccatctatcg ccagttttcc atctgctcct gtggttagac
    13441 ctgactgcag aacggttcca ttggcatctt gtaactcaaa aacagctcct tgcaggactt
    13501 ctccgctttg gtcatcgatc tttgtcaaca cgacaccgcc tggtgtaagt cgattttctt
    13561 tcgtcaattc taccaccgct gtctgacttc tttcgatctc aaactcgatt ggtgtcgcat
    13621 ccaattcata ccccgttggc gcttgggttt ctaccagctg atacactcct ggtgctaagc
    13681 cgtcaatcgc cagttttcca tctgctcctg tggttagacc tgactgcaga acggttccat
    13741 tggcatcttg taactcaaaa acagctcctt gcaggacttc cccgctttgg tcatcgatct
    13801 ttgtcaacac gacaccgcct ggtgtaagtc ggtttgcttt cgtcaattct accaccgctg
    13861 tctgacttct ttcgatctca aactcgatcg gtgtcgcatc taattcatac cccgttggcg
    13921 cttgagtttc taccagctga tatg
//
LOCUS       DAZKOU010000075        13778 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000075 DAZKOU010000000
VERSION     DAZKOU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.076"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..303
                     /locus_tag="ACG827_002159"
     CDS             109..303
                     /locus_tag="ACG827_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484282.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            572..871
                     /locus_tag="ACG827_002160"
     CDS             572..871
                     /locus_tag="ACG827_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484283.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(968..1402)
                     /locus_tag="ACG827_002161"
     CDS             complement(968..1402)
                     /locus_tag="ACG827_002161"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0484284.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1634..2344)
                     /locus_tag="ACG827_002162"
     CDS             complement(1634..2344)
                     /locus_tag="ACG827_002162"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0484285.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2334..3194)
                     /locus_tag="ACG827_002163"
     CDS             complement(2334..3194)
                     /locus_tag="ACG827_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0484286.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3199..3837)
                     /locus_tag="ACG827_002164"
     CDS             complement(3199..3837)
                     /locus_tag="ACG827_002164"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0484287.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3852..4151)
                     /locus_tag="ACG827_002165"
     CDS             complement(3852..4151)
                     /locus_tag="ACG827_002165"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0484288.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4194..5678)
                     /locus_tag="ACG827_002166"
     CDS             complement(4194..5678)
                     /locus_tag="ACG827_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0484289.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5698..6159)
                     /locus_tag="ACG827_002167"
     CDS             complement(5698..6159)
                     /locus_tag="ACG827_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0484290.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6177..7244)
                     /gene="ulaG"
                     /locus_tag="ACG827_002168"
     CDS             complement(6177..7244)
                     /gene="ulaG"
                     /locus_tag="ACG827_002168"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0484291.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7462..8232
                     /locus_tag="ACG827_002169"
     CDS             7462..8232
                     /locus_tag="ACG827_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0484292.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8505..9332)
                     /locus_tag="ACG827_002170"
     CDS             complement(8505..9332)
                     /locus_tag="ACG827_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484293.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9900..11135
                     /locus_tag="ACG827_002171"
     CDS             9900..11135
                     /locus_tag="ACG827_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0484294.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12134..12394)
                     /locus_tag="ACG827_002172"
     CDS             complement(12134..12394)
                     /locus_tag="ACG827_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0484295.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12421..13722)
                     /locus_tag="ACG827_002173"
     CDS             complement(12421..13722)
                     /locus_tag="ACG827_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0484296.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4426 a         2827 c         2172 g         4353 t
ORIGIN      
        1 actttcaaat tgattctttt tatttatcat aaagatatag atacataatc attttcactt
       61 acatatattt gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta
      121 gaagtaaaaa caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat
      181 cctttacttg gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata
      241 aagaacgagc tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag
      301 taaatccaat gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat
      361 tatagaatta gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata
      421 ccatatgatt atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat
      481 gaatcaaata tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt
      541 ataatgtatc agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga
      601 actacttaag caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa
      661 agtgacagag tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc
      721 aaaaggaata tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc
      781 tttaaaaaaa aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt
      841 tgataaagag gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata
      901 ctaagtctat cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta
      961 ttatatgtta tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag
     1021 aaataccacc catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt
     1081 tctcagtatc atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca
     1141 aagcttcatt caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga
     1201 tatcttgaat aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct
     1261 tcccttcccc attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg
     1321 cagtgtcttt tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga
     1381 tcataaggga atcaatattc atttttttct tcctttctta tacaattctc cctctaataa
     1441 tacagttctt tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt
     1501 ccttaaaatt cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat
     1561 aaattttctt cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct
     1621 taccctagca tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt
     1681 tatctagtaa atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg
     1741 ccatcagaca aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc
     1801 cccatgtgaa cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt
     1861 ttctttcctt aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta
     1921 cttcttcttt cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag
     1981 tcgtgccata agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat
     2041 gagtatgagt aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga
     2101 gatctgaaga tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca
     2161 ccatttgatc tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc
     2221 ctagctcacg gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg
     2281 gcaatgccag atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag
     2341 acatcatagc cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct
     2401 cttcgaaagt cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct
     2461 agacgtttca aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca
     2521 tcacgaaact ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca
     2581 gtgatataat cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac
     2641 aaattgccta ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct
     2701 aaaaattttg aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg
     2761 ccatatttag aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa
     2821 ttcattgatt tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt
     2881 agattatgcg ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct
     2941 ggatcagcag aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt
     3001 acaccggagc tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat
     3061 cgtgctaacc gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct
     3121 tttacaaggg aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc
     3181 ccaatttgag ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc
     3241 ggccttcatc ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc
     3301 gatcccttca aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa
     3361 gcctagttca atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga
     3421 taacagcgca tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc
     3481 catggcttgt tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt
     3541 ttgtgctgcc tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc
     3601 tgcttttgca acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac
     3661 tagtttttta tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa
     3721 gcaaagaatt gtgcccactt caataatatc tactacatca ccaactttca taacatctcc
     3781 taatgcatgt tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt
     3841 agttccttcc tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt
     3901 tctttgactg acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga
     3961 gtaaacatat tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt
     4021 tttccctctg aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt
     4081 gctttttcca cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact
     4141 aaaattctca tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact
     4201 ctctgctttt ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt
     4261 ttccgatatt gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca
     4321 taacctagga ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc
     4381 ccgatataac caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct
     4441 acttgtatga ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta
     4501 ttggcataaa ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg
     4561 atcgttggtg atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca
     4621 aacaagaccc caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca
     4681 attcctggaa cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt
     4741 tcatcgacga atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa
     4801 gaagtcgtca taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc
     4861 aaataatctt tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa
     4921 gtagcaacca tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt
     4981 tttttgtctt gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct
     5041 aattttgcaa atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct
     5101 tctgtcaatt cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct
     5161 aaaatcagtc ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt
     5221 aaaatccaaa aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa
     5281 cgcagtttcg tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt
     5341 aataaaatca tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca
     5401 gcattttgtc caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt
     5461 ccaaccaaga ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag
     5521 taaccgaccg ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa
     5581 atatagccta ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt
     5641 gcaaaatacg tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta
     5701 attatattct gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc
     5761 aaccaatcca tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc
     5821 tggatctttt gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc
     5881 actttcaaaa cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc
     5941 atcacttttt tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc
     6001 gtatttttct acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc
     6061 gatcaataca agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg
     6121 gtaatgaatc aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat
     6181 aaaaacgacg gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa
     6241 aaattaaact caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag
     6301 atataaggtt taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc
     6361 tcttttggat cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt
     6421 gcattcaatg actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct
     6481 cgtgggtttt caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg
     6541 gcaaataaat tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg
     6601 aataaatagt ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct
     6661 tctggatctt cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg
     6721 atgtctttga ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt
     6781 ccccattttt gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa
     6841 ttttgatgaa ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg
     6901 attaaagcgt ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa
     6961 ttaggctgca tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg
     7021 ccattcccat gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct
     7081 tcatgacttt tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga
     7141 tccactactt ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct
     7201 actaaccact tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca
     7261 tatcgttgtt ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt
     7321 tatatgaccg tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata
     7381 ataaagacag atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat
     7441 gtccctcagg aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa
     7501 gaaattttgg aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc
     7561 cttgatgtat ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag
     7621 atcatacgca attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa
     7681 tctggtcctg aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat
     7741 tatgatacgc tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa
     7801 tctaaccatt tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat
     7861 aattgtagtt atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc
     7921 gacatcgcat taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc
     7981 ggagtcgatg tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa
     8041 atgatggtca acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga
     8101 ctgacatcaa agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa
     8161 ttttgttctg aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta
     8221 gattcatttt aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac
     8281 aaatttatcg ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt
     8341 atccatccaa tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct
     8401 aaataggaat caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt
     8461 cgaataaacg gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt
     8521 tcaatcacct ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata
     8581 tatctttcta aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc
     8641 aagtattcca atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat
     8701 aattggattg ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat
     8761 acaatgcaga ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc
     8821 acataaactt tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca
     8881 gctgcttgta agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa
     8941 aataatgctg catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa
     9001 atttgattta aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta
     9061 taagattctt ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg
     9121 attttgtctt cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa
     9181 agcgcactta agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt
     9241 gcttgttcac aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat
     9301 tcttttagta atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat
     9361 ttctccttta tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa
     9421 taaaaatttt aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat
     9481 ttctttttgt gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc
     9541 atggcgagaa agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa
     9601 tgacgacagc acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta
     9661 tttttaatga cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt
     9721 gttgcgtgtt tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt
     9781 tgtgaaaaca gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt
     9841 gttttttagt atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca
     9901 tgaagacaat tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg
     9961 aggttgccaa agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt
    10021 ttgctccttt gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga
    10081 aaaaacaaga agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta
    10141 ccaaagaaat ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc
    10201 ctcgactgat tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga
    10261 ttcctttagt atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg
    10321 cgctgatgcc ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga
    10381 caaaaaagcg tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa
    10441 aagaaatgct tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca
    10501 tcacaacgcc taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg
    10561 gtccaatttt tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc
    10621 gaaagcaaca agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc
    10681 gtatgttacg caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac
    10741 ctattgacta tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat
    10801 atctgtctag tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag
    10861 ggaccgtcca aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg
    10921 agcaaagctg taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac
    10981 cagtgactga aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc
    11041 agcaggcacg cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag
    11101 aaatcgaaac cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa
    11161 tagagactca tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta
    11221 aatagtggat tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat
    11281 ccttaaaata aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc
    11341 ttagtcaaat tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa
    11401 agaccataat aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat
    11461 cgctttgtag agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt
    11521 taccacttta tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc
    11581 gatgcatact cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa
    11641 tgttctatgt cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt
    11701 cccagcacaa ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata
    11761 ttaccattca tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata
    11821 tttgacttga tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt
    11881 ccattttttc ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa
    11941 aagggctgtg acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc
    12001 ttgcatctgc ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt
    12061 aagaagcaat tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc
    12121 acttttagaa catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat
    12181 tgatagcaaa aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg
    12241 gtacaatgat ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat
    12301 tgatttgaat atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa
    12361 agataaaaac aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac
    12421 ttagacagtc tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc
    12481 ataagctttt acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa
    12541 gataacaaca cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt
    12601 ccatgttggc aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat
    12661 aatagtcaaa actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat
    12721 aggagctcca aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg
    12781 accaacctct ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat
    12841 cgttgctcct gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata
    12901 gggtaattgg gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat
    12961 cagccacatt ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca
    13021 caacaaatgg atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt
    13081 ggttaaaggc tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg
    13141 aatcagccaa gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc
    13201 aacagcaggt ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg
    13261 atatacttcc actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa
    13321 agaactcata ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg
    13381 aacttgtaac ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc
    13441 atatgattta gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat
    13501 cgtcatccta aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc
    13561 aggaaacggt aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt
    13621 tgaaacaacc ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg
    13681 tgggacaagt ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca
    13741 ataaaatatg aaccattcat tgcatgacaa cgcaaaga
//
LOCUS       DAZKOU010000076        13368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000076 DAZKOU010000000
VERSION     DAZKOU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.077"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            192..959
                     /locus_tag="ACG827_002174"
     CDS             192..959
                     /locus_tag="ACG827_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484297.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            949..2991
                     /locus_tag="ACG827_002175"
     CDS             949..2991
                     /locus_tag="ACG827_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0484298.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            3272..3421
                     /locus_tag="ACG827_002176"
     CDS             3272..3421
                     /locus_tag="ACG827_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0484299.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            3432..4946
                     /gene="dltA"
                     /locus_tag="ACG827_002177"
     CDS             3432..4946
                     /gene="dltA"
                     /locus_tag="ACG827_002177"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0484300.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            4943..6148
                     /gene="dltB"
                     /locus_tag="ACG827_002178"
     CDS             4943..6148
                     /gene="dltB"
                     /locus_tag="ACG827_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0484301.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            6193..6426
                     /gene="dltC"
                     /locus_tag="ACG827_002179"
     CDS             6193..6426
                     /gene="dltC"
                     /locus_tag="ACG827_002179"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0484302.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            6429..7697
                     /gene="dltD"
                     /locus_tag="ACG827_002180"
     CDS             6429..7697
                     /gene="dltD"
                     /locus_tag="ACG827_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0484303.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            7838..8920
                     /locus_tag="ACG827_002181"
     CDS             7838..8920
                     /locus_tag="ACG827_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0484304.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            8920..9723
                     /locus_tag="ACG827_002182"
     CDS             8920..9723
                     /locus_tag="ACG827_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0484305.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            9793..10533
                     /locus_tag="ACG827_002183"
     CDS             9793..10533
                     /locus_tag="ACG827_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0484306.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            10544..11548
                     /locus_tag="ACG827_002184"
     CDS             10544..11548
                     /locus_tag="ACG827_002184"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0484307.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(11593..>13368)
                     /locus_tag="ACG827_002185"
     CDS             complement(11593..>13368)
                     /locus_tag="ACG827_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484308.1"
                     /translation="IILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDF
                     KFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDY
                     QEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQS
                     VPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGI
                     LFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFT
                     PAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQT
                     ALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQ
                     LRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQV
                     TNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPL
                     YRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTG
                     TLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         4299 a         2190 c         2702 g         4177 t
ORIGIN      
        1 attgtgaaaa aaggtgaaaa cttgtagctg gtggatttgt tttttgcata tttaattttg
       61 ctgaataaaa taattgaata aaatttttta ttcagtccaa gtaaaagtga caaaaacata
      121 agagcaaatt attcgtagat atgctactat gcaaataaat agaaatgaaa aatgaaagag
      181 gaggagaaga attggaaaag atgatcgaag tgaaacatct aagtaaaagc tacggcagca
      241 aactttctcc cgtcaaagcc ttagataata tctcattcac agtagaagaa ggcgagtttg
      301 ttgggattat gggcccaagt ggtgcaggaa agacaacgct gatgaatatc ttgtccacga
      361 tcaatttgcc tacgatgggc agtgtctcga tccaaggaaa agaaatcaca aaaatgaaaa
      421 acagtgagct cagtgacttt cgtcgaaaaa aactaggatt catttttcaa gagttcaatt
      481 tgatcgatac cctaaatgca aaagacaata tcctattgcc cctagcagtt gaacggatga
      541 ctaaagagga aatggaaaag agagtaagac atgtagctca attgttgaat atagaagaac
      601 tgctcaaacg gtatcccgat gaactttctg tcggacagag acaacgaatt gcagctgcta
      661 gagcacttgt tgttcagcca caagtgattt ttgcagatga accaactggc tctttggact
      721 cgaagtcggc tacagaattg ttgaactact tgaagaccat gaatcaaaaa gaaaaagcaa
      781 cgatactgtt agtcacccat gatccatata cagcaagcta ctgcgataga attttattta
      841 ttaaagatgg tgtgattttt tctgaagttg tcagaagagg gacaagaaga gagttctttg
      901 agaaagtaat agacatgcag gcgacgattg gcggaggagg aaaaatgaat gctgtataaa
      961 ctagccgtta aaagtttttt tcggcagtca cgaggctatc ttgtttattt tttcagtttg
     1021 actttatccg taatgatcta ttattccttc agcgcaatga cctacgatca atctttagtc
     1081 agaagagcaa gtcaagatac ttcgatagat ggtggattga gttttggtgg atttatcatt
     1141 actgttgttt tgcttttttt cgtattcagt gccaaccgat ttttcttgaa tagaaggcaa
     1201 aaggaaatag gcatctatca gctttttggt atgaataaac tgcagatatc aggtatatat
     1261 gtgttagaaa tcatgataat tggattgttc gcctgtattt ctgggatatt actcggtatt
     1321 attttttcca aactcttttc aatgattttg gttcgaatga tggatatgga tcttactagt
     1381 ccatttttta tctccattcc atcagtagta gatactttat ttgctttttt catcatcctt
     1441 ttagcagtat ctgtatattc aatatggaaa atatggcgct atccaatgat tcgaagtttt
     1501 ggagaaaaag aacaagcaga tagttcaaca atgcgcttca ggacaaggca ccgattactc
     1561 gcagtcatcg ggttactact tttaacgagc ggatattttg gagctagtca ttttagggaa
     1621 atcacttcat ggatgatcag ttatcattat cttgatacat tagttttctt ttttcctttt
     1681 ttaatttttt tcgtttgtgt ggtcggtact tatttgtttt tctgttatac atttcggtta
     1741 tttttgatca tattgagtaa atggaagatt tattactatg gtatcaattt tttgataatg
     1801 ggtaacacgc aagttcattt gctcaaaagt tggcgtacaa attctctgat tacagtcatt
     1861 ctcggaattt ctttaatgac aattggcggc acggctagta tctctactat tttatcctac
     1921 caggagaaag tttcctcccc gatggactat cagtttgatg ttgagacaga gaaaaaaatc
     1981 aagccgattc tagcagagga agggcagaag ataactaaag aaatcacctt gcattataaa
     2041 gtcattggga gtatctatag tcaaaatctc gaagaaccta tgtttcaaac tgctaatctg
     2101 atcacggaaa cacaatatca agccttcaga aaagttaatc cagatttgcc gaacatttct
     2161 ttgaaatcac ctggtcatac tgttttgctt gatcaaatgc aaactctttt tcggaatatt
     2221 tcattatatg gatctagtat tggactgcca gataatcaag aattgaagat tcaacaaatg
     2281 cttccaagtg tattaggtga cgaaacaatg gtatatctaa gtcctgtact gattgtagat
     2341 gataccgttt ttgaaaaagc aaaaggtgtg gattatcaag tagtcaatgt agatgtatca
     2401 ggtggaaata atgaaaaaat agatgaacga ctgagcaatg acctgtcgat aaagtggctc
     2461 aatgctgttt attattctta tgatatcgta aatggaagtc tacaaggaga aattagcaca
     2521 aagaaacttc cggatgacca aatgaaggaa gcacaattta gccaagaaag ctctcgtttg
     2581 aatttctctt ccagctattc gaaattgcgt gcagttaatc ggcgaatcgg tatcaatatt
     2641 tttgtcacac tatttgtcgg gatgatcgtg attattgcta ctggtagtat tttgacagtc
     2701 cggcaattag cagaagcaga ggaagaaaaa gaaaattatc acctattgac taagctgggg
     2761 attccacaaa gaaggatcag acggatgatc ttcgagcaaa acgcacttac cttctttcca
     2821 cccatgatat tagggatcac tcacgcagta tttgcaatca atgtcttcac tcagtatata
     2881 aaaactgccg attactggtt ggcttatctt gtgagtggct tattgatcct tatctatctt
     2941 ttgctttatg tcattacgag tcagttatat tgtcggatta tcgaggaatg atatacggaa
     3001 taagacaaga taataaatag aactaaattt tgaataccat ttatttggaa taagaggttg
     3061 tgacacgatt tttgtcacaa cctctttatt ttttatattt tttaaaagta cgagaaatta
     3121 taaagaaaaa agcagtgatt tgatgtgcta tttttataaa agctatgtga caaaactgtt
     3181 agtaaaaaaa cggtttctgt aagctagaca catgtttctt tttgtcaaaa ttagttacac
     3241 tttgtgtgag gttatatgag gagggaatcc aatgaacaaa tggcgttcta atccaaaatt
     3301 gaattattgg ggaaaattca tcattcgaac aattttttat agtgcagtac tgctgttcct
     3361 gatttatctg tatcactata aaaatattca aggaggaact tttatctata acgaatttta
     3421 ggaggaagag tatggaaatc aaaacgatta ttgaagcaat cgatgattgg ggaataaatg
     3481 aacctgatcg agttgcttat caggcggatg agacgcatac ttttggtgaa ttgaaacaag
     3541 catcagacgc acttgcttat tatttagaaa aaaaggtaga aggcgatgga ccaattgtcg
     3601 tttttggcaa tttggagttt gaaatgattg tctcatttct aggggttgtc aaatctggcc
     3661 atgcctattt gccgattgaa gagcatacac ccaaggagcg tatcctatcg atctttcggg
     3721 tggctaaacc atctatggtc atcagtatcg gggactggat agaagatatt ccttcggttc
     3781 ctgtcataac aaaaaaagaa tttcaagaaa tcaagaagat accagtagga ttttatccca
     3841 gaaatagcgt aaaaggtaca gaaaattttt atattatttt tacttctggt actactggag
     3901 aaccaaaagg cgtacaaatc agtcatgata atctagtcag ctttgttcaa tggacgatgg
     3961 aagactttgg gatcaagcct ggtatgcatt tcttagccca agcgccattt tcttttgatt
     4021 tatcggtatt ttctatttat cctgcattag tttctggcgg aatgctgaag ccgttaaata
     4081 aagcagttgt tcaagacttt cgtcaattat tcgcgacatt gccagccttg aagttgaatg
     4141 tgtgggtttc tactccatca tttatggata tttgcttgat ggaacctaca ttcaatggag
     4201 aaaatgtgcc ggaattggac atgtttttat tttgcggaga agaattgacg aaaaaaactg
     4261 cagaaagtct acttgatcga ttcccaaatg cacggatata taatacttac ggtccaacag
     4321 aagcaactgt agctatttca agtattcaga ttgaccagca tgtactagat agttatgacc
     4381 gactaccgat tggctacgta aaaaaagaca cgaaagtttc tattgtccaa gaaggtaaac
     4441 ctgtttcaaa aggagaaacg ggtgaaatca ttattgcagg accaagcgta tcaaaaggtt
     4501 acctgaacaa tcccgaaaag acagctgctg cgttcttttg tcaaaatgac ttagcaagtt
     4561 accatacagg ggatgcaggt agactagatg aatatgggct tttattttat gaaggacgta
     4621 tggatcagca ggtcaaatta catggatacc ggattgaact cggggatatc gaacattatc
     4681 tgctccaaga caaccgaatc aaacaagctg tcgtcgtacc aaaataccaa ggaacaaaag
     4741 tacagcagct ggttgctttt gtcgtattag aaaacaaaac gaaacagccg gattttcagt
     4801 tgactaaatc tatcaaagaa caattactag aagttgtaat ggattatatg attcctcaga
     4861 agttccatta tgtcgatctg ctgcctcaga cagttaatgg aaaaatcgat cgaaaaaaat
     4921 taacagctga ggtgaatcct gaatgatgtt acctcatatg atcccttatg cagagccgtt
     4981 ttattttttt cttttagcca ttgcgcttgt accgatcgtt ctatctttat tgatatggga
     5041 aaaaagatta ccagtttatc aatcagcagt gacacttttc tttttatttg tcagctttgg
     5101 aggtaattat tggcaacagg gagcggcgct gattgggtat gttttgtggc agacgatact
     5161 tgtatggggc tattttgttt atcggcaaag gaaaaatcag acaggatggt tttttgccgc
     5221 cgttatttta gcgataattc cactgttttt aacgaaagtg actccttttt ttgaacatgg
     5281 aaaagcatcg ttacttggtt ttttaggaat ctcttattta acctttaaat ctgtacaaat
     5341 gatcatggaa ataagagatg gcatgatcaa aacatataag ccatttcgtt atatccagtt
     5401 tctgctgttc tttccaacaa tctcttctgg accaatcgat cgctatagga gatttgaaaa
     5461 agatcagctc catccgcctg ttcgtgataa atatgttgat cttttgggaa aaggcgtcca
     5521 aaatattttt gtcggttttt tatataaatt cattatcggt tattattttg gtcaagtact
     5581 gcttcctgta gtgggacgca ttgccttaca gcatggtggt atttcatggg cattagtagg
     5641 ttatatgtac gtgtacagta tgtatttgtt ttttgatttt gctggctata gcttatttgc
     5701 agtcggtaca agctacatga tgggatatca gacaccaatc aatttcaata aacctttttt
     5761 atcatggaat atcaaagaat tctggaatcg ttggcatatg acgctgtcct tttggttccg
     5821 tgattatgta tatatgcgat tgatgttctt catgatgaag aaaaaatttt ttaaaagcaa
     5881 gatcgtaatg tcaaatatcg gttattttgc tttgtttctt ctaatgggta tatggcatgg
     5941 attgacgtgg tactacatcg tatatggact gtatcatgct tgtctgatct gtctgacaga
     6001 tgcatggatc cgatttaaga agaagcataa agagcagttg ccttctaaca aatttacgca
     6061 tggcttagcc gtatttttga catttcaagc agtatgtttt agcttcctga ttttttcagg
     6121 atttttagat aaactttttt ttagctaacg aatagtgaca tattaattta tataacaaag
     6181 gagaaaagga aaatggaaga acaaatttta tcaatcttag aagaaataac aggaacagat
     6241 gaagtaagaa aaaatcgtga tattgatcta tttgatgaag ggttgcttga ttctttagca
     6301 tctgttcaat tattagtcga gctagacgga gagctggata tccaagtccc tgtatctgaa
     6361 ttcgagcgag aggattggtg cacaccgaat aaaatcattc aacaagcaac tgcattgaag
     6421 ggttgagtat gaaaaagaaa ctttttgcta ttttcggccc ggttgtttta gctgttggct
     6481 tactgtttgt gttttttacc tcctcctatc aagtagatat ctctaatcca gaaacgatta
     6541 gacgtgcctc ttcttccatg tcagtgaacg tgttgaaagg agatgcgata aaaaattatg
     6601 cattatctga aaaacaatat attccttttt ttggctcaag tgaattaagc agaatcagtc
     6661 catttcatcc gtctgttctg gcagaaaaat accagcgaaa ctatcgtcca tttttgctgg
     6721 gagcacccgg gacacaatcg ttaagccaat atatgatgat gcgttctgca ggagatgcta
     6781 tgaaaaacaa gaaagtcgtc tttattattt ctcctcagtg gttcgttaaa aatggtgtaa
     6841 aaactgatta ctttaatacg tattattctg aacttcaaac atacgactgg ttgttttcaa
     6901 tgaagaaagt aacgcctgct gatcgctatt tagctagaag attattaact ttctcaaaag
     6961 taaaagagaa tgatacgttg acggcgattc tgcaaacgat caaaaaaggg aagctacctt
     7021 tacctgaatc tttgaatcag ctgcgcagcc aatggaacat gctgaagaga gaggatgaag
     7081 tattcagcaa tattgggttg aaagatcgac agcaaaaaat cgatcatgag tcaaaacgat
     7141 taccaaaaca ataccaagaa acagaattat ccattttagc aaatcaaatt ggagaacgag
     7201 aaacgacgaa taatccattc gggttgaaaa acgattttta tacgcatcgt atccgagccc
     7261 acgagccaga gctcaaacag tcacaaaaaa attgggatta tcgtttttcg ccagaatttt
     7321 ctgattttca gctggtcttg gatcaattag caaaaaacca taacgaagta ttgttcatca
     7381 tcccaccggt taacgagaaa tggagcgatt ataccggact atctcaagaa atgttacaag
     7441 gatttgccaa aaagatcaaa tttcaattaa atagccaagg attcaatcga atagctgatt
     7501 ttgtcaatca agcgggtacc aactatttta tggaagacac gatccatcta ggctggaaag
     7561 gctggttggc tgcagatcaa cagatcagac cgtttttaga agaaaatcat attactgcta
     7621 gtaagtatca tcttgacgat gcttttttta gcaaatcttg gcaacatcaa ataccagata
     7681 aactacaatt aaaataaaga aaacaggcga gtgttgctcg cttgttttct ttatttttga
     7741 atattctgaa taaaagataa aaataccaag tttgcctgaa caaaaatcat aagcaatggt
     7801 ataatcacaa tatgaatata gaaatggggg aaaacatttg gatacaaaag aagaacagct
     7861 acttattgat ttaacttctg caaaaggagt acctggaaat gaagaagagg taagagaagt
     7921 attcagagaa tatgccaaac cattcgctga tgatattttt tatgacggtt taggcagtgt
     7981 gattgccaaa catggtgctg gcggaggccc caaggtcttt atttctggac atatggacga
     8041 agttggtttt atggtaacaa aaataaccga aaaaggattc ttggaattcc aaactcttgg
     8101 aggttggtgg gggcaagtca tgcttgctca gcaagttgaa atcaaaacac gtgaagggaa
     8161 aatcgtccac ggtgtgattg gttccaaacc accacatgtt ttaacacctc aagtaagaaa
     8221 taaaccttat gaaataaagg atatgttcat tgatattggc gcatcaagcc aagaagaagc
     8281 caaagagtgg ggcatacgtc ctggagatat ggtcactcca tatattgaat acaaacgaat
     8341 gaatggcagt aaatatttac tggcaaaagc atgggataat cgtatcggga cagctgtttc
     8401 tctgcgagta ttagagaatc tatctaaaga agcacaccca aatgttttat tcgcaggaag
     8461 cgatgtgcaa gaagaagtag gcttgcgagg tgctcgaaca agtacacacc ttgtaaatcc
     8521 cgatattgct tttgctttag atactggtac agcaggagac actccaggaa tgacaccaaa
     8581 agaagcagat tcgattttag gaaaaggccc acagattttg atttttgatg catctatgat
     8641 cccgcataaa aaattattga attttgttat tgatgtagca gaggaaatgg atattccatt
     8701 ccaatataca gtgatttctg gcggaggaac tgatgcagga caaatgcatt tgacaagaga
     8761 tggtgttccg tctgtagcca ttaccgttcc tgttcgttat ctgcattccc atacatcagt
     8821 gattcatgaa gatgattatt tgaatacagt gaaactagtc acagaattag tgaaacggct
     8881 tgatagtgaa acagttgaaa aaattcggag ttatgaataa tgaataatag aatacctatt
     8941 tctgaatatc ggaaaaaagc acgagtttct ttggaaaatc aatggggaat caatacttgg
     9001 cttgttttct tatctataat atttacgagc gtcatccaga acttttttga cggtttcagt
     9061 ccttttccgg aagaaagcat gcagcagcac ttattgagtt ttctagtcga taatttactt
     9121 ttattcgcat tcaactatgc attgttttac attgcgctag tagttgtacg gggtggcaga
     9181 ccaaaaagta accttttatt ttcagttttc caaagaaagt attttggttc gatacttggc
     9241 atcaatttga tcaattcagt catcaactgg ctactcaaca gtttaatctt tctgcccatg
     9301 tttttaatca gcggagtgag tacttatatg cagctgcttt tcagttcaaa tgcggtacct
     9361 acgggtgtca tgccagaaga ttttcttgat atcagctttg ttttatcgat gatagggatg
     9421 atttttgttt cagcactcat catgagtatt gttggcggtt tattccaatt tgctgcatgg
     9481 acaaagttcg actatcctga tttgtcggtt attcaatgtt tgaaatatgg ctggttttta
     9541 ttgaaagata gaattggtac gtatattttg ttgcagctgt cttttatcgg ctggtatttg
     9601 ctaggagcaa tcgctttgtt catcggtctt ttgtgggtca ttgtttatgt gaatgtgtca
     9661 gttgcagaat tttatgaaca agcacggatt gaaaaaggag gtccagacgc ctattttact
     9721 tagcatgtaa gcagtttatt catcaaaaaa ataacttttg tggtaggata aatctttgag
     9781 ggggaatgta cgatgaaatt aactgtttta ggatgcttag gagcgtatcc ttataaaaat
     9841 caagggacaa caggttattt gcttcaatct gacgggttca atttgttgat cgatgcaggt
     9901 agtgctacgc tgatcaaatt acaagaacac cttgatccac ttgatctaga tgctgtgatc
     9961 cttagccatt atcatcatga tcatattgct gaccttggtg tattacagta ttactggcaa
    10021 cttcatccta agagaaatcc ggagacattg ctttcaattt atggacacac aaaagattcc
    10081 attcactttg aagaattgac gttggaaaat gtctcaaaag gacatcctta ttttgaagct
    10141 gaagaattaa aaattggtcc ttttctgatt acttttatgg aaacaatcca tccagtagta
    10201 tgttacgcca tgcgtatcgt agaagaaaca acggggaaag tattcgtatt tacaggtgat
    10261 tcaggttatt tggaaagttt cgctgatttt gcgaaagatg ctgatttgtt tctagcagat
    10321 acctatcttt ttgaaggaaa tgaacatcat catgcccatt tcacttcaaa agaagctgga
    10381 gaacttgcga aatcagctaa cgtaaagaaa cttgtactta cacacctacc tcagtttggt
    10441 gatttagaac aattgaaaaa tgaagcgcaa aaggcagcag gcaaagggat tgatatagaa
    10501 cttgctgcag ttgacaaagt gtttaacatt taggaggaag aacatgattt ttgtaccaaa
    10561 tgaaaaccac gacccaagaa tcaatttagc aattgaacaa tatttgctgc aagaaatgcc
    10621 tttagatgaa cctattttgc tcttttatat caatgaacct tcaatcatca tcggcagaaa
    10681 ccaaaatacg cttgaagaaa tcaatcgaga ttatgtggaa gaaaatggca ttcatgttgt
    10741 ccgtcgctta agtggaggcg gagctgtcta ccatgatttt ggaaacctga actttagttt
    10801 tatcatgcct gatgatgggg attcattccg tgactttgcg aaagtaacac agccaatcat
    10861 ccaagcactt catgaattgg gtgttgctgg ggcggaattg aaaggccgaa atgatctagt
    10921 cattgatggc atgaaatttt ctggcaacgc gatgtatgct accaacggtc ggatgtttgc
    10981 tcatggaaca attatgttcg actctgatat caatgaagta gtcaatgcat taaaagtaaa
    11041 aaaagacaaa atcgaatcaa aaggaatcaa atcgatccgt tctcgtgtaa cgaatatcaa
    11101 accatttctt ccagaagaaa agcaaaaaat gacaacagaa gaatttcgtc aagaaatctt
    11161 gttgaaaatt ttcggcgtag agtcagtaga agaagtaaaa acgtacgaat tgactgatga
    11221 agattggaaa aaaatcaatc gaatctctga ggaatattat cgaaattggg attggaatta
    11281 cgggaagtct ccagatttca atttctctcg ccaaaaacgt ttttctatcg gttcgattga
    11341 agtccatttg aatgtctcag aaggaatgat caaagaagca aaaatctttg gcgatttctt
    11401 cggactagga gatatctctg acgtggaaaa aaaattggtt ggacagaaat atgataaagc
    11461 atctctatca aaaatagtca gccaaattga tgtgaagaaa tattttggtg cgatagaacc
    11521 agaagaattg cttaccttac tttattgaaa ataaaaaata gcggaatcca catatatgga
    11581 ttccgctatt ttttatacag gattcgggta atttcgagag agttgaacag tgatccgatc
    11641 aatgaaagta tctttttcct gcgttagtgt tcctgtgtaa cgatccgcta cgtctttcat
    11701 catcattaag tcaaaacgtt cctgatcatc taattcttct gtgatgcctc gactaaatag
    11761 gtttgtatcg taatgtgcac tatgttcgca acaaatgacg aaatgttcct cttgttcgtg
    11821 tatcgacaaa taaatttttc gatacagtgg attttcgatg gcatgagttt ctctaaagga
    11881 atgctctaaa gcgtggatca aaagttgagc cagatcgtca tcgttgatag gaagttcttc
    11941 gggaagatct gcttggaagg ttatctgaat attccgtttg gcagctaact tctggtaacg
    12001 cgctaagata agatttgtca cctggtggtt gcaaaaagaa cacagcattt cgaaattttt
    12061 tgcttcttct aatatctttt ccacatagtt tttagctttt gtttggtccg tatgcattaa
    12121 atcttgcaga ttctccatct cttggacaaa ttccaggcga agtgtattta cttcttggat
    12181 atgctgtcga agctgctcat aatgttgctc caacaaatca tttttgattt ttaagaaatc
    12241 tacttgtctc cgatctttgt cggtatttaa gatgtgatcg attagattgt agctgataat
    12301 aacgattaga atgataatat ataaaatcgt agaccaattg atcgttgttt gatagatacc
    12361 taatgccgtt tggatgtaaa tcaagcaatg gataatggct accaagacga tccaaggtgt
    12421 acaagctaca taaaaacggt tattcttata tgcttcgcca atgcatgcaa gtgaagtgac
    12481 taaagtgctt ataacactag caatattagc aatcggcatt aattcaggaa gttctgcagg
    12541 agtaaaagtt gcataaagca gaacgccgat aaaaatcaaa agttggaacg tacaaaaaat
    12601 tccgtaccat tttttatagt aaaacattcg gctccaatag tagcagatga tgaaagctga
    12661 cgttaagata ataaataaat gagataaaaa agaatgaacg aatggttcaa aaagaatacc
    12721 acttaaaata tcttctgata cagcttcaaa tcctaatgcc aaagcaaagc aacttaagag
    12781 aataagtgag tattcaagac gtttttgttt atacatcaag tagatcgtat agaaaaaggt
    12841 ccctatagcg ataaaaacag cgattatcat cgtcaatact tgcggcactg attgtgaata
    12901 aataaaacta atcaatggtc cagttgttcc tagataaact ttcggtggca gtccagcata
    12961 gttctgataa ggagatgaga ctgtaagggt caattttttt ccgatataat ttgctggtaa
    13021 atctatcaca gccaatgaga gtccgggatt gctttcttcc tgataatctt tccgttggta
    13081 taaaatcttt tcgcctagat atacagtaat ccattgatgg tttcctttga ttagtaaatt
    13141 tgcttcgtcc aacggttttt caagtgtcgt ttccataatg aatgtttcgt ctttatccac
    13201 ggtaggaatc gtacgagtgt acttgctgtc aaacttaaaa tcaggatcat cttttgtata
    13261 gaaaacccac gaatctttta tttctttcat ttgattgctg tccgcaggag aaaaaataaa
    13321 ataaaagaaa taagcaattc ctgttaaaaa taacaatgat aatataat
//
LOCUS       DAZKOU010000077        13248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000077 DAZKOU010000000
VERSION     DAZKOU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.078"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..631)
                     /locus_tag="ACG827_002186"
     CDS             complement(107..631)
                     /locus_tag="ACG827_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0484309.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(624..1097)
                     /gene="tsaE"
                     /locus_tag="ACG827_002187"
     CDS             complement(624..1097)
                     /gene="tsaE"
                     /locus_tag="ACG827_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0484310.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1220..2200)
                     /gene="pta"
                     /locus_tag="ACG827_002188"
     CDS             complement(1220..2200)
                     /gene="pta"
                     /locus_tag="ACG827_002188"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0484311.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2353..3033)
                     /locus_tag="ACG827_002189"
     CDS             complement(2353..3033)
                     /locus_tag="ACG827_002189"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0484312.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3159..4028
                     /locus_tag="ACG827_002190"
     CDS             3159..4028
                     /locus_tag="ACG827_002190"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0484313.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4054..4569
                     /locus_tag="ACG827_002191"
     CDS             4054..4569
                     /locus_tag="ACG827_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0484314.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4616..5179)
                     /locus_tag="ACG827_002192"
     CDS             complement(4616..5179)
                     /locus_tag="ACG827_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0484315.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5234..5413)
                     /locus_tag="ACG827_002193"
     CDS             complement(5234..5413)
                     /locus_tag="ACG827_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484316.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5490..5999)
                     /locus_tag="ACG827_002194"
     CDS             complement(5490..5999)
                     /locus_tag="ACG827_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484317.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6095..6475)
                     /locus_tag="ACG827_002195"
     CDS             complement(6095..6475)
                     /locus_tag="ACG827_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0484318.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6805..7554
                     /gene="pnuC"
                     /locus_tag="ACG827_002196"
     CDS             6805..7554
                     /gene="pnuC"
                     /locus_tag="ACG827_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0484319.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7875..9092)
                     /locus_tag="ACG827_002197"
     CDS             complement(7875..9092)
                     /locus_tag="ACG827_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0484320.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(9350..10627)
                     /locus_tag="ACG827_002198"
     CDS             complement(9350..10627)
                     /locus_tag="ACG827_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484321.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10706..11350)
                     /gene="pcp"
                     /locus_tag="ACG827_002199"
     CDS             complement(10706..11350)
                     /gene="pcp"
                     /locus_tag="ACG827_002199"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0484322.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11549..12493)
                     /locus_tag="ACG827_002200"
     CDS             complement(11549..12493)
                     /locus_tag="ACG827_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0484323.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12495..13181)
                     /locus_tag="ACG827_002201"
     CDS             complement(12495..13181)
                     /locus_tag="ACG827_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0484324.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3763 a         2768 c         2394 g         4323 t
ORIGIN      
        1 tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg tgtgtttttc
       61 tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat tcaatcaaat
      121 aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct ctaggcatga
      181 ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca ttacgaactt
      241 gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg atgatttctt
      301 ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg ataccgactt
      361 ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt ccaataatcg
      421 tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata taatccaact
      481 cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact aaaaattctg
      541 tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct ggcactgctt
      601 cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc tagaattcgc
      661 tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg cttttctaaa
      721 tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc ttcttctaat
      781 agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc taaccctaaa
      841 tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat ccgaccttga
      901 ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat ccctaatcct
      961 tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt caatatcaga
     1021 ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt cattgttaat
     1081 cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc cagtatatcg
     1141 cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct gctgtttttg
     1201 gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata atcgataatt
     1261 tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg ttcaagcctt
     1321 gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta tagccgatat
     1381 ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact tctgaatctg
     1441 gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat tcgccgtcga
     1501 tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact ttgtctactt
     1561 ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt ggttcgatat
     1621 cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct tgcgcagttg
     1681 gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt ccacgtacca
     1741 tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc tgaagtgctg
     1801 ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct gcaatcccca
     1861 tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct gcttgttctt
     1921 tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc cacttatcgt
     1981 aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca tgcgctgcat
     2041 ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct gctttcaacc
     2101 gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt ttgatcccac
     2161 ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct ccagacgttt
     2221 ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta tctgtatcag
     2281 tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact ataacaggaa
     2341 agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt ctcctaacgc
     2401 aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac gacttgcaga
     2461 taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga tcattttgat
     2521 tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc gttcatttaa
     2581 tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag aatacgcttg
     2641 cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag cccatgacac
     2701 tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt tatagatatt
     2761 tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc catgagcttg
     2821 attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt cataaggagt
     2881 caattccaat gcttcataga tatgatacat atctggatgg atcttttggg acgcatattc
     2941 tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg aggacaaaac
     3001 ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat aaaaagaata
     3061 actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga ttaatatggt
     3121 aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta attgcatcag
     3181 atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat gccgaggcga
     3241 tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga aactatcaag
     3301 aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact ttgaacgggg
     3361 cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca aataatcaag
     3421 caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta gcaaccaacg
     3481 atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc atggtggcta
     3541 cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct aagctggagt
     3601 tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag aatctggaag
     3661 ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca gttggaaagc
     3721 agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat atcgaagtga
     3781 accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag cgtgggattt
     3841 ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg ctgcaagtag
     3901 caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat gccaaatata
     3961 caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg attgcagaag
     4021 acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat tcaggaaaag
     4081 cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc cctgtttttg
     4141 atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat gaatggcgat
     4201 ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga gctgtataaa
     4261 gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga cttttattat
     4321 attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata tcaaatccat
     4381 catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg agattactat
     4441 gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc tgcggtttta
     4501 gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta ccgacttaac
     4561 ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca tctttttaga
     4621 tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg acaactttga
     4681 agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca cttagaccga
     4741 tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat cgcgaaccga
     4801 tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat tcgttgggaa
     4861 acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc tctaagatag
     4921 gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa gcaactcctg
     4981 caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga taatttggat
     5041 cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc ttcaaaaatg
     5101 gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga gcatcttctt
     5161 ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg cagaaaaaga
     5221 aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa taaccatgag
     5281 taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt tctttatcat
     5341 tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa ttagggcatt
     5401 tcattttttc catgttatct caacttcctt tcatctttac tctcttagct tactcttata
     5461 ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg acggtcctgt
     5521 tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag aagtgtccac
     5581 tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc tgcttgcggt
     5641 tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt ttccgtgtca
     5701 tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc ttcacaaaaa
     5761 aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac tgcaaaatga
     5821 atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc ttttttcaat
     5881 gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt gagcagtccc
     5941 gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat ttttttcatt
     6001 cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat gaaaaatcga
     6061 atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct aatggcaatc
     6121 cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct ctaatcggtt
     6181 ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg tctactttga
     6241 aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc gcattagcaa
     6301 ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag tcatctcgtt
     6361 ctttgcggta attttctctg attacttcaa atcctaattt attaacataa aaatcacgtg
     6421 atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg ttcatatgag
     6481 cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat tgtaaatgtg
     6541 tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa aaaaacagaa
     6601 agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt aagaaattat
     6661 attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat tgaaagacta
     6721 gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac acactacatt
     6781 tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc tcatcagcta
     6841 aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg ccaggtcatg
     6901 actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg taccacgctt
     6961 ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat tttaggtatt
     7021 ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt aagtatcggt
     7081 gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa agaatggaat
     7141 cataatatgg cttcaaaaat ccgaaaattc actggaaaaa gctggtttat cgcaatcatc
     7201 agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac agatgatcct
     7261 cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat catcagtttc
     7321 ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca aatggtcctt
     7381 tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa cagctctgtt
     7441 tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca aaaagaacgt
     7501 gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc ataaaaaaac
     7561 tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
     7621 ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt
     7681 ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct ctatttccta
     7741 cgattagtca agtaaaaggt gcaagaaaaa tagctcgtaa acgaaagcga caaaatcaaa
     7801 gcacaccaaa taaagccccg ttcgtaaaaa attttcgatc tgggcaaaga agtggtatac
     7861 tgtaagtggt ttagttaatg gtccactgag accgcgtacg catagtcgta ctctgtatgt
     7921 gccctaacca tagcgtgcat acatttcaga agcttgttca tacaagcaac tgtggcaacc
     7981 ttatctttct tagggatagg ttgctttttt aatttgtagt aataatcgac aatatgatta
     8041 ggagctgcgc gttgctggcg aatcatattt cgaatggtga agaaaataat tttccgtgct
     8101 ttgggattac cacgcttatt gatgcggtct tggccagtat acttacctga atgataccgt
     8161 cggatgtcga ttccgacaaa ggcattcaat tgtttatggg ttttaaatcg tgtgatatct
     8221 cctaattcac cgagcaataa agcggctgtg agtgcgccaa tcccagggaa cgtttggtac
     8281 aacaaaaagc aaggagagcc ttcggctttt ttgatcattt gtgtcgccag tgcttttttt
     8341 tgttcgagta aatcttggag aagttcggcg taatagatcg ttttttgaca gtggatactg
     8401 tctttttcaa cagctggata ggcacactgt gcatagtgga tgatttgatc tgccttttgc
     8461 tcggcacgat tcgcggagat ttttttccgt gtttcgttga tcagtttatt ttttattttt
     8521 gtccgagtag atgccaatac aaaatctgga tggggaaaaa gacggatcaa tgtcaatgcg
     8581 taaggggtca gcctgtttga aaagaactgt tcaagctctg gaaaagtcaa ttgaaggcag
     8641 ttgtgtaaat ccatgcgcag acgagtgatt ttcttctcaa tttcttgata aaaacgggct
     8701 aaatcgcgca tctctaagta aaaattgctt tgtacttctt ttggctgacg ttgattttta
     8761 agatgagtct gtgcgagccg gtgggcatcc gatttatctg ttttccaact gcgtaaggtg
     8821 ctttcttcca tctgtttttt cgcctctaac gggtttaaca aacagtaaga aaagtgatga
     8881 tcttgaaaga acttttcgac tggtctggaa taaatccccg ttgcttcaaa aacaatcaat
     8941 ggacgctcag gcaagtgctg gatctcttcg agtaatgcat gaaatcctct ttgagtgtgt
     9001 tcaatctcgt cttcaaataa gcaggtgtcg tctttgtata ggacactgta gctgtgtccc
     9061 attgaaacat ctaatgcaag aatgtaaggc atttgttttc ctccttttta agttttgaaa
     9121 gaactttact gcttcatttc gattccaatt tcctatacac gggctcatgg ccccaacata
     9181 cgtaaacctg attctaaaat gacagtaaag aagttcggtt tttgataggg actcgtagtc
     9241 ctcaaggctt tgttcgaact ttctttcact cttcactata atctaaaaat agaaaataga
     9301 ggagaacatc attcgtcaat gatgttcccc tctaatctta gtatgttttt tatagtaatt
     9361 ccttcagata ctttcttgcc tcatcattga aacgcagtga ttcttctaac ttctgataat
     9421 attccaacgc ttgtttctga gcttcagtaa aacgataatg ttttcctttc catttgtcga
     9481 ttgtctcttg cacatttcca gattggatat ctttcactac tagttgccat ttttcttttg
     9541 agactgtacg gtctttctta tcagcagatt gtatagcttc tcgttcttta cgcaatactt
     9601 ttaaaaaacc atttctgact atccgaggaa tgctgtcgaa gaacccttct gtaaaaccat
     9661 agcgacaata ttgcttccaa ataaatagat agatttttgg ttggatgaat cgttggtttt
     9721 tcgcaaaata ttccatctga gcagtgactt ctggtgttcg ttgacgtttt gtctttctca
     9781 acacggaaat aaatgtttca aaataagcat taggatgtac tggcacttct tcatatacga
     9841 tggattgcca atctttacgt aaaaatgcgc gatagttgat caagaattct tctacatcct
     9901 ttactaattc ttctgcaaag tcgtcaaata gttctttttt tatctgtcgc ttttttgact
     9961 gcccattgat gtgttcactt attttcttga tttcattttc caatgcttga taatccgcta
    10021 tgtaaatcgg catttctact tggcgaaatt ctgcgagccg ctgagctaat tttatatccg
    10081 gtaaatatgg ctgtttcatc cgacgatttt ggtacaagac aaaacagtat ttttgccata
    10141 gtccttcagg aaaatcgata ttcttctgct taagccataa taattcgtct aaggccagat
    10201 caacttttgt cattccctga tggatcgaag ctgccactgt cggagagaca tgcaggtgat
    10261 cggcaaagtg gttgatgcct agttttagga tctttccggt ttttggcgat ttaacgatgt
    10321 attgcacttt taattctcta ccacactcac aatacaattt ttgctggctt cgttttgttc
    10381 gaaaaaacgg attgatcttg aagtcagaaa acacccaatc tgttgcagct aaataatgtt
    10441 cttccataaa acgagagctg attagatatc ggcggtgctt atcgactagt tcccgctgtt
    10501 ttttcgttaa ttttgcatag atctcccaac gttgactgct tgtgtattgt ttagaaattt
    10561 tgatgggata gcttttgctg atttcttttg gaaaccattc ttcaaagacg gattgccatt
    10621 cttccacggc tgtttctttc ctttctaatt acaaaaagct ggctactaac ataacgttag
    10681 caaccagcca gtgtttcttg ctaaattagt gagttgttcc ggcatttccg gaaatatctt
    10741 ctttattttc tactgctgct tcaactgcat aagtcaaaga agaagcaatg tcttctaaag
    10801 acatagaagg cgtacccact ggacgatcga tcacttgatc gggtgagaat ggcacgtgga
    10861 tgaacccacc tcgaattgcc gggaattgtg tttcgatcaa atgaagcaaa cgatacatga
    10921 tatcattgca gacaaatgtt cctgctgtat aagagatgta cgctggtaga ccatgatctt
    10981 gtacattttt tgtcattgct ttgattggca atgaggtaaa gtaagcggct ggaccgtttt
    11041 cttctaaagc cgtatcgaat ggctgatttc cttcattatc agggattctt gcttctgcta
    11101 gattgattgc gacacgttcg aacgagactg ctgaccgccc acctgcttgt ccgacacaga
    11161 tcacgacatc tggttggtgc tgttggatgg cttctttgac tacttgactg cttttttcga
    11221 atacggtcgg tacttccact ttgatgattt cagcaccgct gatttcatct ggcatttttt
    11281 ttactgcttc ataggctgga ttcactttgt ccccaccaaa tggatcaaaa cctgttacta
    11341 gtactttcat ctgttgttcc tccgtccgat tgatttaaca tatggttatc ttttatcata
    11401 ccattttcct tgaacagcgt aaataaagga aacaatagtt taaataacat aacagatcaa
    11461 acttaatata ttcttataaa aaaatagtgt tccactacaa tgttagaaga ggttgcgaca
    11521 attattctgt cacaacccct ctcttctatt atttaaatag aatcatataa gcaatttgga
    11581 aaactagcat aaataacgca ataaacaact ggtttttgat cactccgtat ttgtctttca
    11641 tctccagcat cgctacaggc acgatattga agttagcagc catcggtgtc agcagggtcc
    11701 cacaaaaacc gcaagtcaat gcaaccattc ctattaacgc tggattcgct ccatgactaa
    11761 atacgaatgg tgcaccgatc ccaaccgtca tcacggtgat ggccgcaaat gcattcccca
    11821 tgattaccgt aaatatcacc atgccaagag cgtaaatgat gatgccgatg ttcacatttc
    11881 cttctggaac gatcgtacct actgcattgg aaatcactgt cccaactcca gcgcttgtaa
    11941 ataccgcacc tagagaagct aaaagcattg gcaacatgct taacggccct actgtgccca
    12001 gcatatctgc tgcatcatct aaaaatgtac ttggtcgatt atctcgagaa aagaacatca
    12061 agattagcat ggctgcaaaa acaccgacgc ccacgccgac taaagcacct aaattggtaa
    12121 atagagcaaa caagatcgca aagacaccga tactcaaagc tggaataaaa attttcattc
    12181 ctagtttgtc tgacattttt tccatatatt cttttgatgg aagccggctt tctcctttgc
    12241 tgactttctt agcaattgct ggtatggtca tcgcaaagat caagccaccg ttcacgattg
    12301 ccggaagcca tcttcctcct gcaataacta cccctaagac cgtccagaaa aaggctgacc
    12361 cataacgatg aggattggtt ttatctgtta aatttcttac tccagtgtaa atcaatacga
    12421 gtcccatcac gatataaagg atctccagta atttatcgcc taataaaata tcaggatttg
    12481 taaaaaagtc catcttattg cccctttcct ggataatatt tcttcgctaa tttcttttct
    12541 ttaagataga agtagacact tgccacaact aaagccgtga tagccactgg aacttccact
    12601 aaagcaagct gtcccaatgt tacgtcatat cctagatctt tcagtgttga ttggactagc
    12661 aacgcccctg ctcctccgat gaatagtact tggccaaaga accagcagac attttccata
    12721 gctgaagcca taccttttag ttcttcttca tgcttaggat ggacttctaa cccttttgat
    12781 tcgatggtcc ccaatgccat cggtaaaatg attggacgaa caaatccagc aactccacca
    12841 aaactcacgt taaaagccgc aaagatcatt cgaaagatcc cataaatatc tacaatcata
    12901 ccagcagata ctttcttgaa tcgcttgatc aaggaagccg ctgattcttt cagaccattt
    12961 ctttcaagcg ttcctgttgc caacataatg atgataaaaa tcgccatccc gcgattgttt
    13021 acaaaacttg tacctaaaag ttctaacatt ccttgaatgc caagaccgcc agtcaaagct
    13081 gtaacaacta aagcaaccat tacgatcaat atcgagtcta atttaaatgc aaacccgaga
    13141 ataacaatca agacgcctaa aaggctaaga taattttcca tatattttcc tccataaaat
    13201 gacaatattt ttttaattat aaagcaaaac acataaaaaa tatagttg
//
LOCUS       DAZKOU010000078        13081 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000078 DAZKOU010000000
VERSION     DAZKOU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.079"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..1069
                     /locus_tag="ACG827_002202"
     CDS             89..1069
                     /locus_tag="ACG827_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0484325.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            1191..2609
                     /locus_tag="ACG827_002203"
     CDS             1191..2609
                     /locus_tag="ACG827_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0484326.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            2680..4149
                     /locus_tag="ACG827_002204"
     CDS             2680..4149
                     /locus_tag="ACG827_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0484327.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            4159..4626
                     /locus_tag="ACG827_002205"
     CDS             4159..4626
                     /locus_tag="ACG827_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0484328.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            4662..5534
                     /gene="scrK"
                     /locus_tag="ACG827_002206"
     CDS             4662..5534
                     /gene="scrK"
                     /locus_tag="ACG827_002206"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0484329.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            5616..8150
                     /locus_tag="ACG827_002207"
     CDS             5616..8150
                     /locus_tag="ACG827_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0484330.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            8306..9079
                     /locus_tag="ACG827_002208"
     CDS             8306..9079
                     /locus_tag="ACG827_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0484331.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            9072..9863
                     /locus_tag="ACG827_002209"
     CDS             9072..9863
                     /locus_tag="ACG827_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0484332.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            9873..10364
                     /locus_tag="ACG827_002210"
     CDS             9873..10364
                     /locus_tag="ACG827_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0484333.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            10382..10792
                     /locus_tag="ACG827_002211"
     CDS             10382..10792
                     /locus_tag="ACG827_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0484334.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            10796..12196
                     /locus_tag="ACG827_002212"
     CDS             10796..12196
                     /locus_tag="ACG827_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFQ0484335.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            12219..12593
                     /locus_tag="ACG827_002213"
     CDS             12219..12593
                     /locus_tag="ACG827_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0484336.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         4530 a         1770 c         2638 g         4143 t
ORIGIN      
        1 ccgaaaaggg gaatgagaga gtcaaggttc ttggaatgtc gaatagtcga gtaggtagat
       61 tttctatcta caaccttgtt gtttaaaaat gaaaaaaatt ggaattagag aagttgcgaa
      121 agaggcaaat gtttctccta cgactgtgtc gagggtattg aatgatcgtg gatatttgag
      181 tgaggaaaca aaaagaaaag tttttgatgc catgaaaaaa ttagaatact atcctaatga
      241 actggcgaga gcacttttta aaaacgaaac atataccgta ggtttgattt ttcctacaat
      301 tactaatcca tttcacgctg agttaattca agatatcgag ttcgagcttt ccagcagggg
      361 atataaagtg ttgctgtgca atagcttaaa caatccaaca aaagaaaaag agtacttgac
      421 gatgttgaga aagaatcagg ttgatggaat tatagtagga acgcacaata aagatattgc
      481 ggattatgat attccagggc tacctattgt agcgattgat agaagtcttg gtgaaaatac
      541 agttacagta tcttgtgata attacagggg tggggaatta gctactcaac tacttgtaaa
      601 tcatggctgt cagaatatac tgtgtattcg gggagatagt aagataaagc tacccgcgaa
      661 tagtaggact ttagcatata aagatgtttt gaataaattc atgctggaac cgttagtcct
      721 agaagtacca tttgtaaaag aaatcaatga aaaaaatgaa ataattaaga catatctgga
      781 aactcatcca aatattgacg gggtttttgc aggagatgat ttattggctt ctttggcgat
      841 taacaactta ggaagttcgg gcaaaagagt tccaaaagag gttaaagtga ttggatttga
      901 tggagctcaa caaacattga tttataatcc agaattatca actattcaac agcctataga
      961 tcaaatatct aaagtagcag taaaaaaatt attgaatatg attaagggcg aaaaggagac
     1021 ggatgaactt tcacttccag taaagctcat aaaaaaaaat acagcataag ttaaatcggc
     1081 gaaaaattcc ttcgtcgatt taaaaatatt tttatgaaac gggttgacat atgaaacggg
     1141 ttgacatata ttagttgtga agataaaaac cttttcactg gaggaagaaa atggagaaat
     1201 tattaattaa aagcaataat catcaacttt actaccaacc taaggatgtt tgggtcggag
     1261 atgttatgcc ttacgggaaa gaaggaaaat tctttattta tcatcaacgt gatactagaa
     1321 atccagttcc ttttggagaa ccgtttggct gggctttagc aactacgaaa gattttgttc
     1381 attttgaaga ctttggggaa tctcttttaa gaggggatga caatgagaga gaccagttta
     1441 tctacgctgg ttctgtattt gaggcaaatg gaaaggttca cgcattttat acagggtata
     1501 atcgtgaatt cttagcgcag ggaaagacat ctcaagtgtt gcttcatgct gagagtgatg
     1561 attttgttca ttgggaaaaa tcagaaacag cattggagtt agaaccacaa gaagggtatg
     1621 atattcgaaa ctggagagat ccatttgtcg tttgggatga tgaaaaagaa gaatatttgc
     1681 taattttagg ggcgcgaaag ggtcaggata aacatctaca aaccggacgt ttagtggaat
     1741 ttacatcaaa agatttaact aattgggaat tcaaaggtga tttttgggct ccgggattgt
     1801 ataccatgtt tgaaatgcca gaacttttca agattggtga ctggtggtat cttgtctatt
     1861 cagaatactc agatggaaat aaaatactct atcgaatgag tcaaagtttg gaaggtcctt
     1921 ggattaagcc taaagatgaa gcttttgatg gacgggctta ttatgcaggt agaacagctt
     1981 atgatggaaa acgtcgagtc ctatttggat gggtaccaac aaaagatcaa gaaaatgata
     2041 aaaacaatta tttatgggga ggtactttgg ttcctcatga agtctatcaa aaagaggatt
     2101 tttcacttgg ggtaaaaccc atagacgaag tgtggaatgc attcataaat ttacatgata
     2161 ttgccgatgt agagatagaa acagcagaca caagagaaga agtaacgctt gtcaaaaata
     2221 gtggaacttt gtttaattta gaaacaacaa tttcatttga tgagaatctt agtgactttt
     2281 cactgagatt atataaaaac cttgagacag atgaatctta cgaatatcgc ttcaatttag
     2341 atgaggggcg tcttacattt gataaaaacc cttgttaccc ttggtttcaa tatatggata
     2401 aaggacttat tagacctata tctttaaagc ctaaccaatc ttataaacta aaagtgattg
     2461 ttgatgacaa tataatgaca ctttatattg atggaatcgc tcttaacgct cgtgtacatg
     2521 gttgttttgg tcaagcaatt actattacag caacaaatgg atcaatgact gcaataaata
     2581 ccaagttttc aaatgaaata aagaaataat aaacgtcaat catgtggcag gtctgaaaat
     2641 caaacatgac ctgtcacatt taaaagggag gataaatgaa tggactacaa aaaaattgcc
     2701 gaagatgtat tggaaaacgt tggaggaaaa gagaatatct caagcgctac acactgcatt
     2761 actcgtttac gtttaattct taaagacaaa agcaaagcga atatggaaat gcttgaggaa
     2821 atagaaggtg caaaaggtgt tcttttcaat agtggacagt tacaagtaat tttcggaaca
     2881 ggtataattg aaaaggttta caaagagttc gttgaattga caggagctaa ggaagtatcc
     2941 gtttctgaaa tgaaagacga aggagtcaat aattcaggaa atagacttca acgagctttt
     3001 aaagttttct cggatatctt tattccgatc attccagctt ttattggggc agcaatggtt
     3061 ttaggaattc gttcgttgat taccactcct ggaatgtttg gaatggatgg ctcattcgct
     3121 gatcacaatg tattcctaaa tgatttatcc aaatttctgg gtattattgc aaccacattt
     3181 gattatttac cagttttagt tatgtattct gcaacaaaac gttttggcgg aaaccctatt
     3241 ttaggattac ttgtaggatt tgttatgatt catccaggac tagccaatcg gaatgatttc
     3301 gtcatgggaa ctataaagcc tgattattgg catttatttg gattaagcat tccagcagtt
     3361 gcgtttcaag ggggtgtttt tccagcaatt ttgacttctt ggtttttagc aaaagttgag
     3421 aaaattacaa ataaatattc tcctcaagta cttgcttaca ttttagtccc aactgtgaca
     3481 attgttcttg ccaactttgc actattttta gtgtttggtc cattggggaa ttttatcggt
     3541 aatgttttag gtggaatcat tgatttccta tatatgaaga tgggtgcctt tggtgctttt
     3601 gtttttgcag gcgcgttaca accattagtt gtaacaggaa cacaacatgc tattcaaggt
     3661 attgaggcaa acttagtcgc aacaactgga ttcaattata tccaacctat ttggtcagtt
     3721 tcaattattg cccaaggtgg cggttgtatt ggtatgtatt tactctttaa gaaaaaatct
     3781 aaagatagag atattgcgat ttcaagcttt attccaacac tggtaggtat tactgaaccg
     3841 gctatctttg ctgttaattt gaaatattca atcgttcctt ttgtttgctc gtttattggt
     3901 gctggttttg gtggagcatt tatgaaaatt tttgatgtaa aagccattgg acaaggattg
     3961 actgtattgc caggtctgac tattgctaat ccagttcttc cttatatatt tggtaatctc
     4021 atagcattca ttttaccaat catttttatt ctcgcttatg ataaatataa agtgtttatc
     4081 cctgaagcta aacaccgtaa atttgcagaa aaaactgact caaaaaaatt gccagttcaa
     4141 gtaaagtgag atgagagcat gttaaatttt tttagaaaaa atgaaataag cgttgtagca
     4201 cctgcaaccg gtgtttttga aaaactggag aaattaagtg atccagtctt ttcaaaagga
     4261 atgatgggac aaggatttgc aattgatgca aaaaacgaaa cgattgtatc tccaatcgat
     4321 ggttttgttt caagtatttt tcctacaaaa catgccttag gaataaaaac aaaatcagga
     4381 attgagatac ttgttcatgt aggaatagat acagttgagc taaatggtga gggatttgat
     4441 attaaagttc aagaaggaca aaaagttaag gctggggata ttcttatgac cattaatttt
     4501 gtagtttttg aaaagaatca caagactaaa gatgtcattg ttgtttttcc agactaccaa
     4561 gacgaaattt tcattccgaa acttgaagaa gtaaaacaag gacaagaatt gtttgttctt
     4621 ggctaatgat aagtaattaa taatagaatc gaggaaaatg tatggttttt ggggctattg
     4681 aagctggtgg aacaaaattt gtttgtgcca ttggagataa agatttgaac atatttgaaa
     4741 aagtcagctt tcccacgact acacccaaag aaacaatgaa tttagtttta gattttttta
     4801 atcatcataa agatgattta gaaggtatcg gagtgggatc gttcggtcct attgatattc
     4861 attgtgaatc taaaacgtat ggcttcatta catcaacgcc aaaacttgct tggcaggatt
     4921 ttgattttat tggaagttta aaaaagcaac ttaatattcc aactgcttgg acgactgatg
     4981 taaatgcctc ttgctatggt gaatatgttt ctggaaatgg cagaggcctt tcaagtgttg
     5041 tttactatac cgtaggaacg ggcataggag gtggcgctat ccaaaacgga aacttcattg
     5101 aaggttttag tcatccagaa atgggacata tgcttgtaaa acgacacaat aaagacaact
     5161 tttctgggac atgtccattc cacggagact gtttagaggg cttagctgct ggtccggcaa
     5221 tcgaaagaag atcagggaaa aagggacaag aagtgactga aaatgacgtt acttgggaaa
     5281 tagaagctta ttacattgct cagtgtgcat ataatacaac attgatgtta tcacctgatg
     5341 tcattatatt tggtggcgga gtaatgaaac aaaaacactt aaaaaagaaa gtacaagata
     5401 agtttattga gttgatcaac ggatatgtcg ctattcccga tgttgacaat tatattatga
     5461 caccaaaatt gttagataat tctggtattg taggttgttt agctttagca agagacgtta
     5521 aagaaaaatc ttaatttgga aaaaagatcg tctcgactat ttgtttggtt gatgcgatct
     5581 tttttttcaa ttagtaattg aagggacgcc taaaagtgaa aaaaaaaatg aaagatgaag
     5641 tattagagta tatcaaaaat aatttaaagt attacgattt ttctgctcaa gatattgcaa
     5701 tgaaattttg tattaaaaga aatgtcgctt cccactattt gaatcagtta ttttcagatg
     5761 gaaaattact aaagaatgat tctgttagac cagtaatgtt caagtataat caacaaaagc
     5821 caaaagactg cttttctaag tttattggag cagacatatc tttgaaatcc actatagata
     5881 aatgcaaagc tactgtaatg tatcccccta atggcctccc attaataata aaaggaaaca
     5941 gtggcgttgg aaaaagtttt ttagcaagtt tgatctatca gtatgctctt gatagaaaag
     6001 taattcataa tgacgcaaaa tttgttgttg tgaattgtgc agattatgca aataatccag
     6061 agctattgtc agcagtttta tttggatata aaaaaggagc ctttactggg gctgaaaaag
     6121 atactgcagg gatgctatct tctgctaatg gaggatacct ttttttagat gaggttcaca
     6181 atctaagtgc agaaaaccaa gagaaattgt ttcttcttat ggattctcaa aagtatcgta
     6241 aacttggtga aagtgtgcaa tgggagtatg caaatgttcg ccttatctta gccacaacag
     6301 aagataaaaa tacctctttg ttagcaactt ttagaagacg catcccagct gaaatcactc
     6361 tgccagatta caataataga agtacatctg aaaaaattca attattattt gaatttctta
     6421 aagatgaagc aattacaatt gattcaaaaa tattttgttc gattgaactt tttactgatc
     6481 ttttatcgaa aacatttgaa ggaaatgttg gagaattgag gaatgaaatc aagcttcttt
     6541 gtgcagaagg ttatttagga aataagagaa agtcttcaat ttatttaggg gacaaactag
     6601 acagcggttt ttggatagat ccagaaatta gtatagattt aaaaaaaatg tttcttaaat
     6661 cactttctga aattgatttg attgaacttt ttcaaaatca catttcaatt gaagaaagca
     6721 tgagtcaatt aagagataga atacttaaag agtgtgcagg ttcttctgag tataattatt
     6781 tagaaagtga cgaatttata gcattaagaa actatgttgt taataaaata tcaccaatta
     6841 ttgattcgac aggtctatgt ttattagatg aattcttggc ggatatcagt ctatttataa
     6901 tgtttattga tgtaattgaa gaaacatcaa gaaatttttt gctgtctaag ttcagaaaat
     6961 ttcgtatgaa aaatgataaa caaaaattat tggcaaatga gatttggagt agtttagaag
     7021 tcgaagaaag taaacaagaa cttttgttga aatggatact ttttcttgta aagaagttct
     7081 atgtgaaaat accagaaacc cattgtctga tagttatgca cggtaaaata acagcttctg
     7141 cgatcgcaag ggaaacaaat aagttgctga atacatatgt ttatgaggct ttcgatatgc
     7201 caattgaagg tgagactagt gacttaatta acctaatcaa caatttttgt aaaagtatag
     7261 atacgacgaa tggactcatt ctgctagttg atatgggatc attggaacaa atgtatgaaa
     7321 aaattgaatc caatgtaata ggtgatttgg taattttaaa caatgtttct acagctttag
     7381 cgattgaatg tgggattcaa gtttgccaaa aaaagccaat tagtcatttt taccagatgg
     7441 attttgattc gtttcaagtt aaagttcagt attataaagg attatcacaa aagaaaaatg
     7501 taatagtatc ttgtctctct ggtgagggga tttctgaaaa agttaaggat attttaaaac
     7561 ggtatctcga taatcaagtg gagattttga tttttgattt taacgcatta aagaaaatag
     7621 caaaggaaaa agatactatg atttttaaga atacaatttg tgttctttca accactgaat
     7681 tttacattca aggaatcgat tgtttaaatt tagaaaatct aattaatggg aatcaaacct
     7741 tagaaaaatt aaataagtat atggactcgg atagctgtga gttctgctta aatgaattag
     7801 ttaaactctt tactattgaa ggcgctagta ctaagcttcg ctttttagat tccaaaaaag
     7861 tattcaatga gatagaaaaa gtactatatt tgtatgaaaa atattacgat gtcaaaatcc
     7921 caagttttct gagaattaat ctatttctac atctttctgg aatgattgaa cgaattatga
     7981 ttggtgatgg aataacaaat gaacatttga aagagggagc gcaactattt gatacttttt
     8041 taaatgtatc aaaagatttt ttttcagaaa tcaaagatat ttactgtatt gaaataccca
     8101 aaggagagta tgagttaatc tatacaattt ttgagcagac tattttctaa atagcatact
     8161 agcgtctaaa aaaatgaaag ggtgtccaaa gtatatttag acgctcattt aattaatatt
     8221 tactgtattt tcccgcttta ttggataatg ttagttggca cagtaattgc ttatataaaa
     8281 gtataagaag aaaggagaga acaaaatggc gttttggcaa acatttttag ttgtgttgta
     8341 tggatttttc attaactggg ataaggaagg gcccaaaatc ggaacctccc aacctgtcgt
     8401 tgcaggtttc ttgaccggac ttattatggg agatattcaa actggacttt atatcggtgg
     8461 gactttgcaa ttaatgacac taggtattac tagttttggt ggtgcatcag taccggatta
     8521 tcaaacagca gcattgatcg gtacttattt agcaatctca actaaccagg atgcttctat
     8581 aggaataact ttagcaatac cggtagcaat gttaattgta caactagatg ttctaaagtg
     8641 gtcaacaaac attttttttc aacaaaaggc agaaaaattc gcacaaaaag aagattatca
     8701 aaaattagag tggatgcaat atttggcatc aattaataca agctttgtat ctggaattcc
     8761 tgttttgtta actgtgatat taggcccaac gttagtggga agtgcaatca attatattcc
     8821 tgaatgggtt accggtggtt taaaggtagc agggggacta ttacctgctg ttgggatagg
     8881 aatgctactt aaatatttac ctacaaaaga ttactttgcc tatttgttag ttggtttcgt
     8941 tttggcagtt tatttccaaa taccaatttt agggatttcg ttacttggcg ctgcaatcgc
     9001 actaatcgta tataaaaata gatcaaataa tactcaagta gcaccagctg caacaggagg
     9061 ttttgacgaa gatgaataag aaactgacaa aaatcgacaa acaagtaatt ttccggtgga
     9121 ttctcaatgg tgcaacttca ttaaattatg aaaaaatgga agggttgggt tatgcgtatt
     9181 ctatgcttcc ttttatcaag gacacatata aagatgatcc tgaaggaatg aaacaatccg
     9241 ttttagctca cctacaattt tttaacacta cgccgtatac agcgccttac attattggta
     9301 tgaatgttgg aattgaaaaa gaagaaaaaa ataagtcttt ggaagcagta agtgctctga
     9361 aaacaggtct aatgggtcct ttagcgggaa ttggagatag tgtatttgta gttattccat
     9421 ggactatttt tggagcaatt gcagctaata tggcattgga aggtagtttt gttggaatta
     9481 ttttgtggat actggcatca attttattaa ggattactgc ttatccttta taccttgctg
     9541 ggaaagaatc gggtgcgagc ctactaactc aaattgaaac aaaattaaag tctattactg
     9601 atgcagtatc aattttaggg ttaatggtgg tcggtgcact cattgccaca gttgtaaaag
     9661 tgaatgttgg ctttgaattt aaacaaggcg acttaacaat gaccggaaat gaaatctttg
     9721 atcaagttat gcctggttta ttacctgcat gtgtagtagc aattgtgtat tatttgttag
     9781 gacgaaaggt taaaccagtt tatgtaattc tattaatctt agtagtttct attgcgttaa
     9841 gagcgttaaa tattttgaag taggtgttga atatggatga aaaaatgaaa ggtattatac
     9901 atgttcgtat tgatgatcga atgattcatg gacaagtagc aacacaatgg acaaatcaat
     9961 tgagtgctaa cagaattatg gttattaata atgaagtagc caatgatgac gtaaaaaaag
    10021 ctgtagttcg cctcgctgca ccacctaatg ttagaacttc aataattaca agggaagtag
    10081 cagttaaaaa tattctttct ggtaaatatg aaggacaaaa agttcttatt gttgcagttt
    10141 ctccaatcga tgttaaattt ttgattgata acggtttacc aataacaagt gtaaacgtag
    10201 gaaatttatc aagaaggaat ggaacagaac gcatccgtcc gacgataaat attactgaag
    10261 aagagagaga ggcttttaaa gaactaatta gtgatggagt tgaagtgaca gtgatacaaa
    10321 ctcctagaga ttcaaaagaa tttttaaagg attttattta ttaagaacag aggatgagtg
    10381 gatggagttt ataattacat ctcatggaaa tattgccatt ggatttgtag atacattaaa
    10441 aatgtttttt ggcgataatt taaattgtca tgtggtaaca cttggagatg caggcattga
    10501 agaattccgt gaaaacattg aaaaaatagt ttctcaagta aaaaatcaag aagttatggt
    10561 atttgctgac ttgattggtg gaacaccttt caacgaattt gctaaaagaa gtgaagtatt
    10621 tcaaaatggg tttcaacttc ttggtggcat gaatttagga attttgattg aggctgtcaa
    10681 tttgagatta caaggaaaaa aaagcgatga tatcatcgaa aatctaagaa atatgaatac
    10741 aattgcgtgt cttgcagata tgcaaagaca tactaacgaa gaggatgagt gataaatgca
    10801 aaaaattttt tacaagccta aagatttttg gtttggggat tgcatgcctt tttataaaga
    10861 tggaactttt tatctatttc atcaacgaga tacgagaaat cctaaacctt taactgatcc
    10921 ttttgggtgg tcactagtta caaccactga ttttgtgaaa tttgaagaat acggggaagt
    10981 aattcatcgt ggtggcaacg atgacattga tcaatacatt tacgctggaa gtattgtcca
    11041 aggaatcaat gagtatttag ctttgtatac tggacataat cgcaaagcta agttggatgg
    11101 aaaaacttca gaagtgttaa tgattgcaag ttctaaagat ttaattcatt ggaaaaagga
    11161 tggcgaagca gcaaaacttg ttcctcaaga aggatatgat aagaatgatt ggcgcgatcc
    11221 ttttgcaatt tttgatcaag atcatggaaa atacgttttg atactaggag ctagaaaacc
    11281 agcaagtaag aaaaagccga cggggagatt ggtctatttt gaatccgaag atttaaaaga
    11341 ttggaatttc aaaggagact actgggctaa taatgaattt aacatgattg aaatgcctga
    11401 tatatttgaa attgatggaa cgtggtattt acttttttca gagtattgtg aagataaaaa
    11461 gactaaatat cgagtcggag caggattatt ttcagattgg aaatcaccag tagacgaagc
    11521 tttcgacgga aaagcctatt atgcagcaag aacagtcgga actaatgaaa ctggccgttt
    11581 tctaactggc tgggtggcaa caaaagaagg ggagaacgat caaaataact gggattgggg
    11641 cggtgcactt gttccacatc aaattattca aaagacagac aaaactcttg gtgttaaact
    11701 gcctgaaaaa ataattgaga attttaaaga aatcactcca ttttcaagtt ttaagttaga
    11761 gaaagaattt ggaaaaaaag aaagagtaat tgttcctaaa atcgaagatt attttctatt
    11821 tgaaacaact ttgactatta catcgttaac aaaggagttt gctttaaaat tatttgtaga
    11881 tagtgatact caagaaggat acgaatttcg tatttcattg attaataatc agcttacttt
    11941 cggaaaaact ccgaactatc cttggcctca aatgtttgat aaggggctgc aacgaccact
    12001 atttttggaa ataggcaaag aatatgaact aaagttaatt gttgatgata cgatagctgt
    12061 tttatatatt gataatgttg ctttaagtac tagaatgtat gataaagtcg gaacagcatt
    12121 aagtatgact gttacggagg gagcaatcaa ggtagataaa ctagactatt caataaatta
    12181 tgaatgtgga ttttaaaaca attgaaggga tgatgtaagt gaaattaaat ggtttacaac
    12241 atattggaat tccaacaaaa gattttggag cttcaaaaat cttttatgaa tccttagggt
    12301 ttagtctgat taatgaagaa atgaatgtgg gaagtaaagt cggctttttc gagttgaatg
    12361 gaacgataat agaaatatgg gaagataaaa ctactgagtg tagaggagcc attgatcatc
    12421 tagcattaga tacggatgac atagatgagg tattcaattc aattcaagat ttaggatatg
    12481 aattattaga taaaaatatt atggaattac cgttctggga aaatggaatc cgttacttta
    12541 atttctacgg accaaatcat gagattatag agataagcca aatgaagaaa taaatagata
    12601 acgaagtgct ctaagttggt atgaacgaaa agtacttcat tttttggtat cagcatttca
    12661 atagtttaat gagaatttac tatttcaaat taattcaaag tgacagtatt ttttacttat
    12721 ttggtttcaa attttgtaat ccttcctatt tattggaata cactccacat tttgtataca
    12781 agtcatatca tgttttagca aagctcttac caagcaaagt tataatgatg ttttccaaag
    12841 tttgatcttt cgatggaggc taacacatat tttttgcagg acagccaaaa ttagatgtat
    12901 agaatagagt ctgtgaggcg tatagacagt ttattgtctg tatgcctttt tttattttga
    12961 aaaattttta gtatttttcg aaaaatgctc actcctagag taagcgcccc atagaacagt
    13021 accttactaa caaatgcacc tcaagatata cttgagctat caagaagtct ggaggttttc
    13081 t
//
LOCUS       DAZKOU010000079        12916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000079 DAZKOU010000000
VERSION     DAZKOU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.080"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG827_002214"
     CDS             133..3660
                     /locus_tag="ACG827_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0484337.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG827_002215"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG827_002215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0484338.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG827_002216"
     CDS             7448..7789
                     /locus_tag="ACG827_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0484339.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG827_002217"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG827_002217"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0484340.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG827_002218"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG827_002218"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0484341.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG827_002219"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG827_002219"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0484342.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4387 a         2244 c         2739 g         3546 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttaca
//
LOCUS       DAZKOU010000080        12747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000080 DAZKOU010000000
VERSION     DAZKOU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.081"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG827_002220"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG827_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0484343.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG827_002221"
     CDS             complement(323..892)
                     /locus_tag="ACG827_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0484344.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG827_002222"
     CDS             1078..1980
                     /locus_tag="ACG827_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0484345.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG827_002223"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG827_002223"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0484346.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG827_002224"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG827_002224"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0484347.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG827_002225"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG827_002225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0484348.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACG827_002226"
     CDS             8554..8739
                     /locus_tag="ACG827_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0484349.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACG827_002227"
     CDS             complement(8801..9997)
                     /locus_tag="ACG827_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0484350.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACG827_002228"
     CDS             complement(10167..11603)
                     /locus_tag="ACG827_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0484351.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACG827_002229"
     CDS             11896..12705
                     /locus_tag="ACG827_002229"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0484352.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DAZKOU010000081        12635 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000081 DAZKOU010000000
VERSION     DAZKOU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.082"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1111)
                     /locus_tag="ACG827_002230"
     CDS             complement(146..1111)
                     /locus_tag="ACG827_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0484353.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="ACG827_002231"
     CDS             1345..2190
                     /locus_tag="ACG827_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0484354.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="ACG827_002232"
     CDS             2381..3568
                     /locus_tag="ACG827_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0484355.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="ACG827_002233"
     CDS             3574..3930
                     /locus_tag="ACG827_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0484356.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="ACG827_002234"
     CDS             3932..4270
                     /locus_tag="ACG827_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0484357.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="ACG827_002235"
     CDS             4665..5699
                     /locus_tag="ACG827_002235"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484358.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="ACG827_002236"
     CDS             5692..6378
                     /locus_tag="ACG827_002236"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0484359.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="ACG827_002237"
     CDS             6402..7250
                     /locus_tag="ACG827_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0484360.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="ACG827_002238"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="ACG827_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0484361.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="ACG827_002239"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="ACG827_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0484362.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="ACG827_002240"
     CDS             9516..10751
                     /locus_tag="ACG827_002240"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0484363.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /gene="sufU"
                     /locus_tag="ACG827_002241"
     CDS             10738..11208
                     /gene="sufU"
                     /locus_tag="ACG827_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0484364.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="ACG827_002242"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="ACG827_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0484365.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT         4179 a         2366 c         2798 g         3292 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atata
//
LOCUS       DAZKOU010000082        12514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000082 DAZKOU010000000
VERSION     DAZKOU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.083"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..788
                     /locus_tag="ACG827_002243"
     CDS             51..788
                     /locus_tag="ACG827_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484366.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            802..1617
                     /locus_tag="ACG827_002244"
     CDS             802..1617
                     /locus_tag="ACG827_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0484367.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1630..2277
                     /locus_tag="ACG827_002245"
     CDS             1630..2277
                     /locus_tag="ACG827_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0484368.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2293..2949
                     /locus_tag="ACG827_002246"
     CDS             2293..2949
                     /locus_tag="ACG827_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0484369.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3111..3932
                     /gene="racE"
                     /locus_tag="ACG827_002247"
     CDS             3111..3932
                     /gene="racE"
                     /locus_tag="ACG827_002247"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0484370.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3935..5290
                     /gene="rph"
                     /locus_tag="ACG827_002248"
     CDS             3935..5290
                     /gene="rph"
                     /locus_tag="ACG827_002248"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0484371.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5291..5809
                     /locus_tag="ACG827_002249"
     CDS             5291..5809
                     /locus_tag="ACG827_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0484372.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5916..6416
                     /gene="cbpB"
                     /locus_tag="ACG827_002250"
     CDS             5916..6416
                     /gene="cbpB"
                     /locus_tag="ACG827_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0484373.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6696..7616)
                     /locus_tag="ACG827_002251"
     CDS             complement(6696..7616)
                     /locus_tag="ACG827_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0484374.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7742..8794
                     /locus_tag="ACG827_002252"
     CDS             7742..8794
                     /locus_tag="ACG827_002252"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0484375.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(8904..9659)
                     /gene="fabI"
                     /locus_tag="ACG827_002253"
     CDS             complement(8904..9659)
                     /gene="fabI"
                     /locus_tag="ACG827_002253"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0484376.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9879..10319
                     /gene="fabZ"
                     /locus_tag="ACG827_002254"
     CDS             9879..10319
                     /gene="fabZ"
                     /locus_tag="ACG827_002254"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0484377.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10434..11195)
                     /locus_tag="ACG827_002255"
     CDS             complement(10434..11195)
                     /locus_tag="ACG827_002255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0484378.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11384..12478
                     /locus_tag="ACG827_002256"
     CDS             11384..12478
                     /locus_tag="ACG827_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0484379.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         4042 a         2173 c         2637 g         3662 t
ORIGIN      
        1 tatacatctt taaaagaaat cttactaaat aataatggga gtgtaattct atggcaatga
       61 ttaaatttga tcacgtggat aaatattatg gcaagttcca cgcattaaaa aatatcaatc
      121 tcgaattcga gaaaggagaa gttgtcgttg tcattggtcc ttctggttca ggaaagagca
      181 cgatgcttcg ctgtatcaat ggattagaga cgatctcttc aggtaagctg ctgattaatg
      241 atactgactt gcatgataaa aagacaaaac tgacagaagt ccgaaaaaac atcggcatgg
      301 ttttccaaca tttcaatttg tacccaaata aaactgttct ggaaaacatc aaattagcac
      361 caatcaaagt gttgaagcaa gaccaggcaa ctgcagtcaa aaacgcggag aaatttttga
      421 aaactgtcaa tatgttagat aagaaagatt cttatccttc catgttatcc gggggacaac
      481 agcaacgggt ggcaatcgcc agaggtttag cgatgaatcc tgagatgctg ctgtttgatg
      541 aacctacttc tgctttggat ccggaaatga ttggtgatgt attagacgtt atgaaaaaat
      601 tggctcatga cggtatgtca atgattgtcg tgacacatga aatgggcttt gccaaagaag
      661 tggcggatcg tgttatcttc atggctgatg gagaagtatt ggaagacagc agaaatgtga
      721 gagacttttt cgaaaatcca aatgaagtcc gagcacagca atttatcagt aaagttatca
      781 atcattgata ggaggaaaaa gatgcgcagg ttaaaaaaaa tacgcctgat tttcctttta
      841 ggcattgctt ttcttgcact agccggatgt aaaggaaatg ggttggcaaa tgaagatatt
      901 ttagagcgaa gcaaatctac caatgaaatc atctggggtg tcaagtatga tactcgatta
      961 tttggaatga tggatattga gagcagaact gtacaaggat ttgatgtaga tattgcaaaa
     1021 gcaatcacta aaaagatcct tggtgacaat ggaaaaacag aatttgttga agtcacttct
     1081 aaaactcgga ttccattatt gaaaaacgga aatatcgatg caattattgc aacgatgacg
     1141 atcacagatg aacgaaaaaa acaggttgac ttttcagatg tctatttcga tgctggccaa
     1201 gctttgttgg taaagaaagg aagccaaatc aaaagtgtag atgatttgaa tgcttctaca
     1261 acggttctag cagtcaaagg ttctacatcg gctgcgaata tccgacagca tgcaccagat
     1321 gcaaaaattt tagaattaga aaactatgca gaagcattta ctgctttaca atctggtcag
     1381 ggagacgcga tgactacgga taatgcgatt ttgttaggga ttgcagatga aaatccagag
     1441 tatgagttag ttggtggaac gtttactaat gagccttacg ggattgcgat caacaaaggg
     1501 caagaaaact tcttgaaagc tgttaaccaa gcgctagagg aaatgcatgc agatggcaca
     1561 tacgataaaa tctatcaaaa atggtttcca aatgaaactg aaggaaaagt agagtaaggg
     1621 ggagaggcaa tgagcgaatt gatacaaact tatggaccaa tctttgtcga cgggttcaag
     1681 atcacattgt tttcgagtgt cttagcactt gtgtttagtt tgattatcgg aacgttaatg
     1741 gcaatcttcc aattatcaaa gaataaagta gtgagcgcct tagcaaaggc atatgtagaa
     1801 ttttttagaa acattccatt attaatcatc gtgatgtttt tttatgttgt cgtaccgatt
     1861 tattggttta atctagatgg attccaagca ggtacgattg gattaacgat ctatacatct
     1921 gcttttattg ctgaaacagt acgttcaggg attcaaaccg taccaaaagg tcaaacagaa
     1981 gcaggactat cttctggttt tacctattca gaaatcatgc gttatattgt tttaccgcaa
     2041 gcattcaaaa tcgttatccc gccattaggc aaccaattta tcaacctcgt aaaaaactca
     2101 tctattttag caatcgtagc tggtttagat ttgatgtatc aaggagactt gatcgctagt
     2161 gaaacattca ataccttcga tacgtatatc atcgttgggt tattttactt agttatcacg
     2221 ctaccgttat cttacttaat ggtttatctt gagaaaaaat gggcggttcg ttcatagaag
     2281 gaggggaaat acatggattt tagcggcgct ttttcatgga tgaatatccg ttttttattg
     2341 gaaggattaa aggttactgt tgaagtttca ttgttttcga tcatttttag tttcttgatc
     2401 ggtggtctga caggtgtgat tcgttttgcg aatatccctt tcttttccaa aatccttggt
     2461 gtagtgattg atattatacg gaatcttcca ttattattga tcattttctt cacttacttt
     2521 gccttacctc aaatcggcat tcagatgaac atcttctggt ccgctgtcgc agcattaacg
     2581 atttttgagt cagctatgtt gtcggaaatt ttccgagctg gactcaatgc tgttccaaaa
     2641 ggacagatgg aagcaggatt atcaacggga ctgacttatg tagaatcgat gcgaacgatc
     2701 gtgttaccgc aagcatttaa atcaatgatc ccggcaattg tgagccaatt gatttcactg
     2761 atcaaagata cttcattagc agtaatcatt tctttacctg aactttctca tcaggcacgt
     2821 attatttatg gacagaacac caattatgtg cttccgatgt tcatcatcat gacacttatg
     2881 tacttcgtgg tatgttacgc gctttctctg ctttccggtt acttggaaaa gagacggtac
     2941 agttattaat agatgattct tttagcggtt gtggcaaact aaagtcacaa ccgcttttcc
     3001 tctttccatt cgaaaaaaag agatttcgat tgttttaagc aagtaagagc acttgaaaat
     3061 tcacgctaaa aagtacacaa tagggaggat agaatttatt gaaaaaaagg atgatacgat
     3121 tgacagataa tcgccctatc ggatttattg attcaggtgt cggcggcttg actgtagtaa
     3181 aagaagccct gaaacaatta ccgaatgaaa atattttatt tgtaggagac acagcacgct
     3241 gcccatatgg ccctagaccc gcggaacagg taatccagta tacttgggaa atgacggatt
     3301 atctggtgga gcaaggaatc aagatgctgg tgatcgcctg caataccgca actgcggtgg
     3361 ctttagaaga aatcaaagct gctctttcta ttccagtcat cggtgtgatc cttcccggta
     3421 ctagagcggc agtaaaaaaa acacaaaata aacaagttgg cattatcggt acgattggta
     3481 cggtaaaaag tcaagcttat gaaaaagcac tgaaagagaa agtaccagaa ttgactgtga
     3541 caagtcttgc ttgtcccaaa tttgtttcag ttgtcgaaag taatgaatac cattcatcgg
     3601 tggcgaaaaa aattgtggca gaaacattag ctcctttaac cactaaaaaa atcgatacat
     3661 tgattttggg atgcacccat tatccattat tacgccccat cattcaaaat gtaatgggag
     3721 aaaatgttca actgatcgat tctggagcag aaacagtagg tgaagtatct atgctgttag
     3781 attatttcaa tttgagcaat tcaccgcaaa atggtcggac attatgccag ttttatacaa
     3841 ctggctctgc caaacttttc gaggaaatag ctgaagactg gcttggaatc ggacacttaa
     3901 atgtagaaca tatcgaattg ggaggaaaat aaggatgcgt catgacggga gaaaagccca
     3961 agagcttcgc aaactggagc ttcaaacaaa tgttttcaaa tatccagaag gatcggtagt
     4021 gatccgtttt ggtgatacaa cagttatttg ttctgcaaca atagaaaatt ctgttccccc
     4081 ttttttgaga gaaacaggaa caggatgggt gacagcagaa tatagtatgc tgcctcgagc
     4141 aacgagtacg agaaatcgta gagaaagcag caaagggaaa ctttctggta gaacaatgga
     4201 aatccagcgt cttattggtc gttccttgcg tgcagtagta gacttggaaa aattaggaga
     4261 acgtagtatc atcgtagact gcgatgtgat ccaagcggat ggcggtacta gaacagcaag
     4321 tatcactggg gcatttgtgg cattgaaatt agccattgaa aaacttttaa gagaaaaaga
     4381 attgtcagaa gatccaataa aagaacatct tgctgcagtt agcgtaggaa tcttaccaga
     4441 tgggacttgc gtcacagatt tggattacca agaagattcc gcagcacttg tagacatgaa
     4501 tcttgtaatg acagaatctg gaaaattcgt ggagattcaa ggaactggag aagaagcaac
     4561 gtttgatggt gaacagttga atgaaatgct cttttttgga aaaaacgcga ttgaagattt
     4621 gatcaaagag caaaaacatg ctttgctaac agagtttgct caaaatgatg agcggataga
     4681 agaaacaaaa acaatcatca ttgcgacaag aaatccagga aaagcagaag agtttcgaaa
     4741 tatgttcaaa gaagcgggat atcatgtaaa aacattactt gactatcctg aattgccaga
     4801 tgtggaagaa actggcagca catttgaaga aaatgcccga ttaaaagcag aaacgattgc
     4861 acaattgctt gatcagccag tcttagcaga tgattcaggt ttgaaagtgg atgctttagg
     4921 cggtatgcca ggaatctatt ctgcacgttt tgcaggggaa caaaaaagtg atgcggggaa
     4981 taacgccaaa ttattatatg aattgacaga tgtgccggat gaaaaaagaa cagcacaatt
     5041 ccattgtacg ttagtattcg cggcacccaa aaaagacagc cttgtagtag aagcggaatg
     5101 gcccggtcga gtagcccgta taccatctgg cgaaaatggc tttgggtatg atcctttatt
     5161 tatcccagaa ggtaagaaac aaacagcagc agaattatcg agcgaagaaa aaaacaagat
     5221 cagtcatcgg gctcaagcaa tgaaaaaatt gagtgccgag tggaaacaat ggctggaagg
     5281 agagagatag atgcggtatt tagtagtaag tgataatcat ggagacagag atatcctcgt
     5341 tgacttagta gatcgttatc gttctgaagt ggatatcttt ttccattgtg gtgattcgga
     5401 attagaagct tctgacccat tatgggatac attccaagtg gttcaaggta attgtgatta
     5461 tggaccagga ttcgaacaga aaaaagtgat ccaaactggt caggatacag tattcatgac
     5521 tcacggtcat ttatcgaatg tccgatttgg tttgacacag ttagcaatcg aagctcgagc
     5581 tgcaactgcc gatatggtgt tttttggtca tacccatcaa ataggctgtg agatggatca
     5641 gaaaatcctg tttttgaatc ctggtagcat ttctcagcct agaggcccga tccaaatccc
     5701 atcatatgct cttatcgaaa gtttagatga ccaaatttct gttcaatact ataaccgtgc
     5761 ccatcaggca gttgaagagt tggccttttc tttttcgaga gagcgctgaa taaaaaaact
     5821 ctaattttta ataaaaataa gctttatttt atgaattttg catccggttt cggtaaaata
     5881 gtagtgttga atgaaataga aatggaggcc atcagatgat tggacctata gtaagagaaa
     5941 tgctgctgga aaatcaggaa acgtttttga ttcctgcaga aaatgtggca aatgttatgt
     6001 atcatcatcc attgtcccat ggtttacttg tgttatcgaa ggtcggttat tccaaaattc
     6061 ctgtattagg aaaagatgat cactttgtag gactagtaag tttatcagat gtggtcaata
     6121 aaatgattga cttgcaaaca atcagcatgg acccgttaga aggactaacg gttgctgatg
     6181 tgacagaaac agatgttccg acaattgatg aaaattggga actagaagaa gtactccatt
     6241 tgttagtcga ttcgcctttt ttaccggttg tcacagaaga tcggattttc aaaggaatca
     6301 ttacaagaaa agagctgctt aaagccgtaa acttcatggt gcatgaatta gaacgtcgta
     6361 atatattgtc tccgaaaatc gaagaattaa aagaacaaat cgatatcgtg agctaaagaa
     6421 cgagaaacta aaagataatc aaaaaataag tagaaaaaac agcgtaccag ttttgacttt
     6481 atcttgaaaa agaggctggg acaaaagcgt ttaactccaa gaaataagaa ggaattcacg
     6541 aaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt
     6601 ccgccgttta tacgtgttta gagcgtggga caaaagtgtt ctttactttt gtcccacgct
     6661 ctttttagtg agattcgttg catcgtttgt ccaaattatt ttccgacagc aattggactg
     6721 ttcggaatcg tgaaaccaga agctgtcaat tctttgacat aggagctcaa gaactcttct
     6781 tttaaggcat attgttttcc gttcaatgcg tagcagaccg tacgaatcgc aaaattgctg
     6841 tttccaagat ccactaatcc aaagatagaa ggttcagttt ggagctcttc ttggtatttt
     6901 tcagctaatg tacgatttac tcgatcaatg atctcataga ttttgtcata tccttcttca
     6961 ggaacaatcc gaatatctaa aagtacttgc atatttgcac gagagagatt gctgatcgtg
     7021 gtgatgtttc gattcgggat gaaatggaca gtaccatccg ttgctttcag ctgaagggta
     7081 cggataccaa cagatacgac ggttccttca atcgttaagt ttgctagctt gatgtagtcc
     7141 ccaacatcca tctgttgttc catgataata aaaaatccgg taataacatc actcatgaac
     7201 ccttgggcac caagaccgat tgccacacct gcaattccgg caccagccaa aagtgatccg
     7261 atcggcacac cgatagcaga taaaattgca tagataaaaa agaaaccaag ggtataatga
     7321 aaaatagtat ttaaaagact atgtaaagtc tttaagcggc tctcgtttag aatggctttt
     7381 ttagaatact gattaaaaga cttatcaatc agatattttc ccacacggtg aagaatccaa
     7441 aaaaggacga tcaaaaaaat cagatataaa cttttttgga taaaggcagc caaaatggcg
     7501 tcccaatcaa tcccgtccca aaaccgctga aacgcattta ctttgctgac agcttgatct
     7561 gtcaatgtct cgccagaaga gtctgtcgta ccggcagttg ctaataatga aaacataaaa
     7621 tcctcttttc taaaatttga ttctttctgt attgtaccaa gaagttttca aaaaaggaaa
     7681 tagcaaaaat aactataatg aaggaaaaaa gctgaaaata aaatgacaaa taatatggat
     7741 aatgataaaa tgtggagaaa ataacgaagg tggtaagtat atgcaaatcg attggaacaa
     7801 tctaggtttt tcttatatca aaactccttg gcgttttatt gccaaatgga aagacggaca
     7861 atgggaagaa ggagaattga cagaagataa ttacctgacg attcatgaag gctctccagc
     7921 gcttcattat ggtcagcaat gttttgaagg tctaaaagct tatcgtcgaa aagacggcaa
     7981 ggtcaatctc tttcgacctg aacaaaatag tcgacggatg aatcaaagtg ctagacgatt
     8041 gctgatgcca atggtccctg aaaagatgtt cgttgatgct gtcaagcaag tggtaaaagc
     8101 aaatgaagat tttgtgccgc cctatggaac aggcgctaca ttatacttgc gccctttact
     8161 gattggggtc ggtgaaaaca ttggtgtcca tccagctcca gaatatttat ttgtcgtctt
     8221 ctgtacgcca gtaggagcgt atttcaaagg tggactaaaa ccaacgaatt ttattgtatc
     8281 tgattacgat cgtgctgctc ctaaggggac tggtgcagca aaagtcggcg gaaattacgc
     8341 agcgagttta cttccaggaa cagaagcaaa agaactggat tactcagatt gtgtttatct
     8401 tgatccagcc acacatacga aaatcgaaga agtaggtgca gcaaatttct ttggaattac
     8461 aaaagacgga aaattcatca ctccgcaatc gccctctatc ttaccaagta tcacgaaata
     8521 ttccttgttg cagttagctg aagaacgact aggactgcaa gcaattgaag gagatatttt
     8581 cattgatgat ctagatcaat ttgcagaagc tggtgcatgt ggtacagcgg ctgttatttc
     8641 tccaatcggt gggatttatc acaataacaa tcttcatgta ttttatagtg aaacggaagt
     8701 aggaccagta acaaaacggt tgtatgatga attgacaggt attcaatttg gagacataga
     8761 agcaccagaa ggctggattg aagtcgtgga ataatttcaa agtaatgagt tgaaaaaaac
     8821 gtaaaaagaa aaaatcagga agagacttta aaaagttttt tcctgatttt tatatgtttt
     8881 actttaaaga atgcaacaaa agtttatgaa agatggacac ctttgtcaac gtagataata
     8941 tcaccgatga ttccgctgga aagtgggctg attaagaaag cacaagcatt accgacttct
     9001 tcgatcgtca caccttgctg atcaggcgta cgagattcag ataactggat cagtttttga
     9061 tagtctttaa cacctgtgac agctaatgtt ttgattgccc cagctgagat tccattgacc
     9121 cgaattcctt taggcgagaa ctcagctgcc aaataacgga tagcagcttc aagagaagct
     9181 ttcgcaatac ccatcatgtt ataattcggg attgcgcgtt ctgaacctag ataagtcatg
     9241 ctaacgatcc cgctgtttgg tgcaagaaac ggttgtgcgt attttgatac agcaattaaa
     9301 gagtagctgc tgatatcttg agctaattga tagccgtctc tggaaatatc agacacattt
     9361 ccggacaatt cttctttgtt ggcgtaagca actgcatgga caaggccgtg gatctcccca
     9421 gcatattcac cgatggcatt cattgctttt tcgatgcttt cgtcgctaga aacatcacat
     9481 tctacagtga atgcttcttc gccgaccaat ttgactaatg attttttcat tcgttcattt
     9541 tggtaagtat agataatttc tgccccttgt ttcaacattg cttcagcaca accccaagca
     9601 atgctttttt tgttagcgac gcccataatg acgatttttt ttccttctag aaatgacata
     9661 atgactctcc tttgtttctg ataaaaattt gatagtcgta aaaaaataga gaataatagc
     9721 taaaataact tcttgatttc ttaattatta gtcaaaatgt tttgattgtc aaactatttt
     9781 ttcttaaatt aaaaaaagat tgctaaatat ttaggaatat gatactttga tagtcaaagt
     9841 aatgaaaatg gcaattgcct gaaagaagga gtttaattat gtcttacgta ttatctgcta
     9901 ctgaagtaat ggaattgatt cctaatcgtt atcctatttg ttatattgat tatgtagata
     9961 ctttagaacc aggaaaaaag atcgttgcga caaaaaatgt aacaatcaat gaagattttt
    10021 tccaaggtca ctttcctgac aatccagtga tgccgggtgc gttgattcta gaaacactgg
    10081 cacaagcagg ttcgatactt atcctaaaat cagaagagtt tctaggaaaa agagcttata
    10141 tcggtgggat caataaggcc aaattccgtc aaaaagttgt tccaggagat gtgctgaaat
    10201 gtcattttga aatcaccaaa atgaagggat cagtcggtac agcgttatct gcagcatatg
    10261 tagatgataa aaaagtctgt gagtgcgagt tcacgttcat cgtcaaccaa aatgcttgac
    10321 tataaaacct gatatagcaa aacaaaagga agcttacttg ctttttatgt aaaaagaaga
    10381 cgcctgaatc ctagcatacg ttgtcatcta tgcaggattc agacgttatt tttttatttt
    10441 tgttttgctt cttgcagtat attgatttta tcgattttta cttcttcttt tggtttgtct
    10501 gaactatctg tttcgatatt tgcaatagca tccacgacat ccattccttc aatcacttgg
    10561 ccgaagacag tataatggcc atctagtgaa ggataaccgc cgtttttata agcgtcgatg
    10621 attgcttgag gatagtcgtc ttttaaaaga ccatcatgca tatcatctgt attttgaacg
    10681 ataaagaact ggctgccgtt actgttagga tcttgtgagc gagccataga taacgcacct
    10741 cttatattat aaaggtggtt gttgatctct gtttcaaatc cttctcccca aatgctttcc
    10801 cctccagtgc catcgccttt aggatcgcca ccttggatca tgaaatcttt gatgacgcga
    10861 tggaaagtca gtccgttgta gtaaccgtct tttgcatgtg tcataaagtt ttcaaccgct
    10921 tttggtgcgt gttcgggaaa taatttgatt ttgattgtgc ctttattcgt caccatttcg
    10981 accagatctt cattttcggc gacatctttc gtcaattgtg gtaattccaa tgcgttcaaa
    11041 tcgacgctac tggcagtcgt gctttccgca gtagatgtac tttttgaact ttctgtagac
    11101 gaactagaag aagtcgtgtt gtttgcagaa caaccagcag caaacagcaa tgctgctgct
    11161 aaactaatac ttaaaataac tttatttttc ttcataactg ttcctttcca atttactatt
    11221 ttgttatctt taatgataac aaaatagagg gaaaatacct acttaaatta ttggaattta
    11281 tcatttattt taaaagaaaa agaaaaggtg tagaccattt actttgtgtt tgctataatg
    11341 taagtggata caaaaacgaa aagatgcagg aggaaatggt aatatgggaa aattaggaat
    11401 ctcgatttat ccagaacggt caacatttga aaaggacaaa gcgtacttgg atttagctca
    11461 taagtatggg tataaacggg tttttaccag tttgctgcag atcaatgatg ataaagaaaa
    11521 agttctttca gaatttaaag aagtagttga ttacgcgaat agcctaggca tggaagtaat
    11581 ggttgatatc aatccagcgt tatttgaaca gttggagatt tcttacgatg acttatcttt
    11641 tttccataag atgggtgcct acggggtccg cttagatatc ggattcacag gagcagaaga
    11701 agcaaaaatg acgcgcaatc cttttggtat caaaatcgag atcaatatga gttcgggcac
    11761 gaattacgtt gacaacatca tgagttattc gccaaacacg gataatttat taggttctca
    11821 taatttttat cctcatcgat actctggttt aggatacgaa cactttgttt tttgttcaga
    11881 aaaattcaga aaatataatt tgaatacgat ggcattcgtc aattcgcaat ctgctgagtt
    11941 tggcccttgg cctacgcaag atggcttgtg cacgctggaa gaccaccgag atcttgaaat
    12001 tgcgacacaa gtgaagcatt taatccttac aggattgatt gatgatattt ctattggaaa
    12061 tgcgtacgca agtgaagaag aattgaaaga aatggcagaa gcatttaacg cggattatcc
    12121 aacattgaaa gtagatacgg aagaaggtat tacagaaaac gaacgaatct gcttgtttga
    12181 taaccttcat agttatcgag gggatcgctc agaatatatc cttcgttcaa cgatgactcg
    12241 tgtatactac aaagataaag atttcccgcc tcataatacg cgagatatgc atcatgggga
    12301 tgtactgatt gacaatgaag ggtacggtca atacaaagga gaaacacaaa tcgccttgaa
    12361 ggatatgaaa aacgacggac gtgtcaatgt tgtaggaaga atctccgatg acgaattatt
    12421 cctccttgac tttttgaaac catggtcaag ctttaaatta attgaaaata aaaaatagat
    12481 aattagttat tgcagaaata atccgctaga aata
//
LOCUS       DAZKOU010000083        12493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000083 DAZKOU010000000
VERSION     DAZKOU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.084"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG827_002257"
     CDS             <1..375
                     /locus_tag="ACG827_002257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484380.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG827_002258"
     CDS             complement(324..893)
                     /locus_tag="ACG827_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0484381.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2257)
                     /locus_tag="ACG827_002259"
                     /pseudo
     CDS             complement(967..2257)
                     /locus_tag="ACG827_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2515..3459
                     /locus_tag="ACG827_002260"
     CDS             2515..3459
                     /locus_tag="ACG827_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0484382.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3473..4546
                     /locus_tag="ACG827_002261"
     CDS             3473..4546
                     /locus_tag="ACG827_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0484383.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4536..6707
                     /locus_tag="ACG827_002262"
     CDS             4536..6707
                     /locus_tag="ACG827_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFQ0484384.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6819..8990)
                     /gene="copB"
                     /locus_tag="ACG827_002263"
     CDS             complement(6819..8990)
                     /gene="copB"
                     /locus_tag="ACG827_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0484385.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9010..11196)
                     /locus_tag="ACG827_002264"
     CDS             complement(9010..11196)
                     /locus_tag="ACG827_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0484386.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11196..11405)
                     /gene="copZ"
                     /locus_tag="ACG827_002265"
     CDS             complement(11196..11405)
                     /gene="copZ"
                     /locus_tag="ACG827_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0484387.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11418..11858)
                     /locus_tag="ACG827_002266"
     CDS             complement(11418..11858)
                     /locus_tag="ACG827_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0484388.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11932..12430)
                     /locus_tag="ACG827_002267"
                     /pseudo
     CDS             complement(11932..12430)
                     /locus_tag="ACG827_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3520 a         2630 c         2217 g         4126 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttt gatcttcgtt atggtctagt ggaaaaaaat
     1081 gatcggtgat cactagatct gcataccgag gcggcttatt tcgagtacat acatgtatag
     1141 agatccgttt tcgatgtctt agcgttttgt tcagtacccc agcaagtctt cttgcttctt
     1201 ggtgttcata gcgaaaaaaa aagacgattt tcacttcagg agggtggaca tctaaatacc
     1261 ctcgttcctg gagtgttttc agtaaaagca tcccccactc tactgcgggg tgtgcaaatg
     1321 cttgttggat cgctagccga aattgatata gtttatcttt ggaattggga aatttttcta
     1381 atgtttcctg ataataacga acttcttcta tcagccggtc atggatacca attacgtaac
     1441 gaagccattc ttggtaaagt acttttgtca gctttgattt gttttcgctc tcaaattcgc
     1501 tattcagcca aaacagcgaa tcaagctttt cagtcacaat cgtcaatttc cgatagtctt
     1561 gtgtctcttc tatccgtttc caccgctttt cagtaatctg gttacagctc aagcgcaaaa
     1621 ttgctagata atttttttct tttattggca aaatctgctc ttctctaaat agcaattcag
     1681 gaaacaattc aaagaatagt gttcgactgt ctcctaaaat ctcgatttct gccggatcct
     1741 ttctttgctt caataattgg atgaacatct cagaaaagga ctgctctcca tcatctgcaa
     1801 aaaaatgcgg aaaatctttc aaaaagcaaa acagcagata tcttactttt ctttctgaac
     1861 caaagatctg tgctttttta taagaaaaag aaaggtggta ctcgccaaac cactttctca
     1921 atcgctcttg cgtagaacga tcgtacttct gctcgtaagg ttcattcaat agcagatttt
     1981 tcagtaaaca aaacgggggt gattggatca gaaaaacaaa caagagataa ggttcgctat
     2041 atccttgatc ataaactaac tgaacggttt tcttatgaag aatcagctgg atgggacagt
     2101 cgctttccgc caaacgtttc agatgccgat ccactgtttt aacatcgatt ttcagttctt
     2161 cagctaaatc agcttttcgc cagatcaaat ctttcattag attgattagt gaaaataaat
     2221 tctgtgcatt ttttgataat agtttttgcc atctcatcga tcatttctca cctgatttct
     2281 aaacatattg tttgatcaaa atgggttcac tcctaaaaag ataaaaaact ccctttctat
     2341 ttatttaaat acgcaaattt tcctcattat ttttaatttt actcaattta atcgtattct
     2401 catttcaaaa cgaatgtact ttaaaaaaac ataataaaat cactttgctt gtaggatgta
     2461 ttcccttcca ttacaatgaa ataaatagca attttatgag aaggtgatgt gaaaatggaa
     2521 gaaaaaatcg aagaatccaa acaaaaaatc gatcagttga tacaagaatt agaaaaagcg
     2581 gtcatcggca aacgttcaac tatccaattg atgatcgtgg ccctattagc aggtggtcat
     2641 gtcttattcg aagatattcc cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa
     2701 gctgtacagg gaaccttcag cagagtccaa tgtactcctg atctgctgcc tagtgatatt
     2761 cttggttttt ccgtatacaa ttcacaatca aaagagtttg aatttcgtcc gggaccgatc
     2821 tttaccacga tcttgttggc agatgagatc aatcgcacga ctcctcgtac tcagtcagca
     2881 cttttagaag caatggccga gaatcatgca acgatcgaca acaatacata ccctctagac
     2941 aatcattttt ttgtcttagc tacgcaaaat ccaatcgagt atgaaggaac atatcctttg
     3001 ccggaagcac agctcgatcg ttttttattc cgactacaaa tcggttatcc ttcttttgat
     3061 gacgaacttt tgctactttt agataaattc gaaaaagtgt tggataactt aaatgaagta
     3121 ttgaacagtt atgaaataga agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc
     3181 gaagtggcat cttatgcctt gcagttagtg tcagcaacaa gaagccatgc tgccatccag
     3241 cttggcatca gtccacgagg aagcctttcc tttattcagg cagcaaaagc ttatgcttta
     3301 atccatggga gaaattacgt cacgcccaaa gatttgcagg atttagttcc ttacgtgttt
     3361 tcccatcgcc tgatttttta cgatcgttca ttgaaggaag acgaaaaaaa acaaatcatt
     3421 catactcttg ttgcacaaat tcctgttcct gtgaggtaga ttgaggtaga taatggaaaa
     3481 aatcaaagat tatttcatta attttgtgtt atttctggta tatttcatca ttgtgctttt
     3541 tgctcttgta ttcaacaatt ctttaggctg gttcttgttg gcctttttta ctctttatgt
     3601 atcatggagc ttgatttcat taagcagctt tctaaagaaa ctttcggtta cttgtacatt
     3661 gcagccactc gtttatcgca aaaaaagtat gcaggttatt ttttccatag caaaaaagag
     3721 aactttttct ttacctctcc ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa
     3781 aaaacaagag attttgattc ttacaaataa accgcaagaa gtccaattgc tttggcagcc
     3841 aaaagaaaga ggatgccatg aaacattaac tgtcattttt acagcctatg atagtctagg
     3901 acttttcaga aaaagtgcaa aaagagagat ctcttttcct gtgactgttc tgcctgcttt
     3961 ttgtaagatt caagcagaaa aattacagag agttctggaa aaaaaacagc agctgaacgc
     4021 gtaccaaaga ggtcttgatt ttcgggaaca tcgcgcttat cgtccaggtg attctttcaa
     4081 ccgaatcgat tggaaattgt ctgctcatac tcaagagtgg ttataccgtg aatacgatta
     4141 tcaagaagaa gaatgttctc ctcttttttg cttttggggt tcgcacagtc caaaatttga
     4201 acctctgcta gacttgtatt tttctttatg gcatttgaga aaagaaaagc agccggaact
     4261 tctgatatta ggcaaccgcg cctttcatgg caaagacccc ggtcatacct attttgcttc
     4321 tttggaagct ggcgatgaga aagctttttt cgcacaccta aaaaaatatg caaaaaaaca
     4381 aatcgtcctc tttgttcctg agaattctcc tgaagtctca gaagctattg aaacattgtc
     4441 aaaagatcga acggtttatc ttgtttattt cgtagaaaaa caactcatgg tccaagaaaa
     4501 agacaagata tgttcattgc caggaggtga atggattgac gattaaatgg gaaaaatggc
     4561 tgtgtggcgt ttactccttt gtgatactaa gtgtaggtgc tccgatattt gtagtagcta
     4621 atcaattgca aaacagctac cttcttatcc taactattgg tgtcctatgc cttattgtct
     4681 gctgtattcc taaaaaatgg gtacgtttgc tgctttcttt gccagtaatg ataggttgtt
     4741 tgtttttgta ttttccttcg aagacatttt ccttattatg gatcttacag tttttttcgt
     4801 cagtcacaga ggaattcttc catctatccc aaggtaaact ttattatttg ccagaaaaga
     4861 cagctttttt tctcataatg ttgctgatct atttatttat tacattgacc gtggattacg
     4921 actatttgta cgcacccttt ctttctttga tggcttactt catgatgctt accgtttttc
     4981 gggaaaaaga tttactgtac gaaatgattg cggtagtaac tgttttgttc gtgtatctgg
     5041 cagcaaggca atttttctcc atgcggttgt tgtcgggaaa cactcgattc cagtcattga
     5101 ctacagttct gcttatttgt ttcttaacta ttagcgctat cttgccttta tatcttccga
     5161 aagtacacca atctctcgag gaaacgagtg aaccgatccg agaatatctt aatgatgaag
     5221 ggctttatga tacactccat gagtatcaat taaccggaag tgcaagaaca ggcttcagtg
     5281 agaatgatga acagcttggt ggacctttat atgataatgg cgaagttgtt tttacagcga
     5341 accaaaaagg aaatggctat tggaagatcg aaaataaaaa tcattacacc ggttctggat
     5401 ggatagaaga tcaggacgca cccatccaac caatccaaga attccctgtt gacatcacga
     5461 gctaccaaac cgttatggaa gacaaagaaa acattacatt gaccctaaaa aaacaagctt
     5521 tcttacctta tccttatgga aatactacct tgactgattt ttcattagct agtggacaaa
     5581 cgctttacta tttgcctgaa agccagcgat tcattcctgt cccgaatgaa tcgacacgtt
     5641 ctgcaacttt acaaacagaa aggttagttt ttacgagaga agaattaaaa agcagtcagt
     5701 taaaagctga tgatttagaa aatcagcaga caaatcttca attgccaaaa agtttgccta
     5761 aacgtattga ggacttggcg gtcgaattga ccaaaaatga agcaacttta attcgtaaag
     5821 tcgaagctgt cgaacagtat ttaaaaacaa gcggaaattt ccgctattcc aaaacggata
     5881 ccggacatac tccgaaagaa cacgattatg tggattattt cctctttgat tcaaaagtcg
     5941 gctattgtga caacttttca acttctatgg tcgtccttct tcgaacactt ggtatcccag
     6001 ctcgctggac aaaaggtttt acaaatgggc tcagaacagg cacagataat caagggaatg
     6061 aggaatacag cattctcaat agtcatgctc attcttggcc agaggtatac tttcctggat
     6121 ttggctggct tccatttgaa ccaacccctt catttgccaa tccaatgggt tcacaaccag
     6181 ttgctgaaac aaccgaaacc gtccagcctg ctatcgaaca accaaatgaa agcgaaaata
     6241 tggcagaaac tatttccagt gagagatctg aggtcattcc atcagaagaa acaacactca
     6301 caccagctta taaaatcgtc ttattcagcc ttggggtgtg tttatacgtc ttgttgttct
     6361 ttctattgtt attcagaaaa tctctgcgct ggacaagtta tcttatctat cttcattttt
     6421 ttgcttctac taatacgatt gcatatcaaa aacttcttaa ccatttggaa acattggtac
     6481 cgcgcaaacc cgcagaatct ttgcaggaat atagcctgcg aatagaacaa gaattcaagc
     6541 tagatctgcg tttcattcga ttaactactc aatatgaagc agaaatatat ggacgacaag
     6601 cagatccggt aaatttcgat aaaaaaacct atttagaaat tacgcatata ataagaaaaa
     6661 cggtatacaa aaaagttctg acaaaaggga gccgcttcaa aaaataagat aaaatttctg
     6721 gagaatctac ttctggaatg ccgagaacca gctgcttgag tatcgattat tcgaaaaaaa
     6781 ggttgtgaca cgttttttgt cacaaccttt tttcattatt atttgatttt caatgtcaaa
     6841 gcatttagag ctacaactac cgtacttaac gacatcaaga cagcaccaac tgctggactt
     6901 aatatgatac caattggcgc aagaattccg gctgctagcg gaattgcaag aatattgtaa
     6961 cctgcccccc accagaggtt ctggatcatt ttctttcgcg tttgcttggc tagatcaaga
     7021 aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga catccgcaga atcgattgcg
     7081 atatctgtcc ctgcaccgat tgccatcccg attgaagcac gggctaaact tggcgcatca
     7141 ttgatcccat cacccaccat gattactttt ttcccttggt caaggtattt ctggacaaca
     7201 gcttctttat catctggtaa taaaccgcca taatattctt cgatccctaa ataatctgct
     7261 actgcttggg cagcgttttt attgtcacca gtaagcatga caggagtgat ttttctttct
     7321 ttcaatgttc ggataaattc ttttgcttct gtttttacta cgtcacctaa agcaacgatt
     7381 gcagctagat gattttctaa aatcaggaag ctgatggtat ttccttgttc ttggtaaggt
     7441 ttcaataatg cttgttctac gtttaatccg agacgctcaa cttctttttc attaactatt
     7501 ttcacttgtt ggtttttgac tgttgcctct agcccaactc ctgaaagatt ttttagattt
     7561 tcggcttgat aaggcttgat ttcatgttct ttcaaataat tcataatacc aacagccaat
     7621 ggatgattgg cattcgcttc caatgctcct aaatattgaa ggatttcatt tttattgaac
     7681 gcatcatcaa gtacttcgac acccgttaca gtaaattttc cttctgttag tgttcctgtt
     7741 ttgtccagca tgatcacatc tagatcattg gcttgctcca gtgcattgcg gtttttcaaa
     7801 agtaatccgt ttttagctgc tattgatgta gagcgagcaa caactaatgg gatagctaaa
     7861 cctagagcat gaggacaggc gatgataaag actgttacca tacgttctaa tgcattagga
     7921 agatctgtca agaacaacca ggcgatgaat gccaggatac ccacaattaa tgcgacataa
     7981 aacagccatt ttgccacttt gtcagataat gactctagct tggatttttc tccttgtgct
     8041 tttcgcacca tttccataac cttcgctaga taaccatttt caccagttcc agttacttcg
     8101 atttcgatcg ttccatcccc attgatagaa ccgccaatca ctttgtcatt ggcttgtttt
     8161 ttgattcctc ttgattcgcc agtcacagct gattcgtcaa caattgtttc acctttcaag
     8221 attacaccat ctgttggcat tttatcacca gaccggacga tcaaacgatc accttcgttt
     8281 acttctttca atgaaactgt ctcttctttt ccatctttcg tcaagcgttt tactgattct
     8341 ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat tcgacacagc attcatttca
     8401 atccagtgac ctagtagcat aataacgatc aaagtagcca attcccagaa gaaatccatg
     8461 acgtgtgtat gtggattgag cagatttgcg ataaacgaat agacactata aatatatgca
     8521 actgtaatcc ccatcgcgat cagtgtcatc atagcaggac ttttttgttt caattccatc
     8581 ttagctccac ttaaaaatgg ctgaccgcca tagataaata gtatagttgc taagatcaat
     8641 acgagccaat cagaaccagg aaacgttact tgaaatggaa attccattcc catcattggt
     8701 gaaaaaagaa tgattgggat agctaggata agcgatagcc aaaatttttg tttgaaattt
     8761 cccatgtgca tggaatgatc catgccgcca tgatcgtgat ccatgtgcat cgaatggtcc
     8821 atttgtttgc tgtttgctat atctccatgg ttatgtccca tgtgcatgga atggtccatc
     8881 tggctatggt catgtttcat ctccatctgt ttttcatgag attctgtact ttctttgttt
     8941 actacttctt ttttatgttc atgttttttc tccatatcgt tcccattcat ttttactcca
     9001 tcccttctct tactttttca tgtgccgatt taagctcagg gagttcaaca atacactgac
     9061 ggaactaaac gccatcgctc ctcctgcaat gattggattc aaaaagccta gtgcggcaaa
     9121 aggaatacca atcgtattat agacaaaagc ccagaaaaga ttttgtttga tttttctcaa
     9181 tgtcacccta gacaactcga ttgtttgtgc aatggatgcc aaactgctac tcatcagcgt
     9241 cacatcggct gtttccatcg cgatatccgt tccacttccc attgccatac caacatcagc
     9301 taatgctaaa gccggtgcat cattgattcc atcgcctgcc ataccgactt ttttgccatc
     9361 tttctgaagt ttttctacgt aggctgcttt atcctctgga agtacttcag caaagatatg
     9421 ctttggatca ataccaactt gtttaccaat cgtttcggct gccagtttgt tgtctcctgt
     9481 cagcataaaa acatctaatc ctttattctg gagctgcttg attgcttgtt tggcttctag
     9541 tttgatttga tcagccacgg caatcaaccc gatcacttgc ttttcatcag ctaaaaacat
     9601 gactgttttt ccctgctgtt ctaattctaa cgcgtgttct tggtattcat caaatgatag
     9661 gttcaattca attaatcgtt tacgtgtacc cgcaaaatag cgtactccgt taatcgtacc
     9721 gctgattcca gcacctgggt gagctgtaaa atcaatcata tcgtatgttg ctgcttcttg
     9781 cagttttccg tattccacga ttgctttacc tagtggatgt tcagaagctt gttctaaagt
     9841 ataaaaaata gataggactt ctttagaacc gactagatct gtaactttgg gctttccttc
     9901 tgtaatcgtc ccagttttat ccaaaacaat gctgtctaag tgagcagctg cttctaatgc
     9961 ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca cccgttccta ccataatagc
    10021 tgttggagtc gctaaaccta atgcacatgg acacgcaatg actaatacag agacactatg
    10081 gatcaatgcg agttgccaat caccagtgat caaacctgta gctatgagcg tgataaacgc
    10141 gatgactaat acgatcggaa caaaaatccc tgagatccga tctgcgattt tttggattgg
    10201 ggctttgctc ccttgagcat cttccaccat ttgaatgatt tgcgctaata ctgtctgctt
    10261 gccgacttgt gtgacttcca tgtgtagcag accattcgta ttgattgtcc cgccaaaaag
    10321 cgtgtcatcc ggattctttt ctacaggaag attttcacca gtaagcatac tttcatctat
    10381 cgcactgctt cctgaaataa ttcgaccatc tgctggaatc tgctcaccag gacggatgac
    10441 tagttgatca ccaacgacta cttcttcaat cgctaacgtt tcttctttcc cgtttcttat
    10501 cacttgggcg gtttttgttt gaagagacat caattgtttg attgcattac ctgttttcgt
    10561 ttttgctgta tgttccaaat atttgccgag caaaatcagc gtgatgatca tactgctgct
    10621 ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc tcaatgcaaa
    10681 ggcagccgat gttccaattg ctaccaatac atccatattt ggtgctttcg acttgattgc
    10741 atggtacgca cctttataaa aacgagcacc gacataaaat tggacaggag cagacagcac
    10801 taactggacg attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc ctaacatcat
    10861 agcaatcatt gacaacatca acggcaaagt caaaaccgta ctcaatataa gatcgaatag
    10921 catttttttc aaatacgctt gcttttcttc tgcaattttt tgtttgtggg cttcatcgta
    10981 caaaattgca ccatagccga tgttttctac gctttggatt aatttttcag tggtcgtccc
    11041 ttcatatttg acacttgctt tttcagtggc caagttgact gtggcattga ccacaccagg
    11101 ttgtccgttc agctcttttt cgatcctagc agaacaattt gcacaagtca tacctgtaat
    11161 gacgaacgta tccattttgg tttctgctgt tgccatcaaa tcacctctgc tttataaccc
    11221 aatccattga ttgcttgaca aatcttttcg gattgaactt ttgtttcatc aaacttgacc
    11281 gtaccgtttg cttttttcaa atttactttc actttttgca cgccgtcaag tgcgcttact
    11341 gtttcttcta ctcttgctgc acaatgattg cagctcatac cttcgattga aaattgttgt
    11401 ttcatttttt cttcctccta ttgatgttgt ttacattcac attgacctgg gatacagtta
    11461 cattcgatcg tatctactgg ttgcttttgt tccagttgtt tcaaaatctc attgatatct
    11521 tcttgagtca aagtggcttc ttctactaga tccgcaatcg tctctccaat cctttttgca
    11581 cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc cgagacagct
    11641 ggatgataaa tgaatttttt tccttcttgt tctgtccaga tcacttcttt ttttaccaaa
    11701 cgacccagca aagttttgat cgttgctggt ttccaatcca tcgaatcagc taagatctgc
    11761 gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat ctcccactct
    11821 gaatcactaa tctttactgg cgttttgact ttttccattt atttcacacc tttcgtttac
    11881 aaatgtaatc attaacttat gaatacagat tacacttgta aacaaaaact gtcaattatt
    11941 tttgctctct attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg ttttaaggat
    12001 gatcagttta tttcttgcat tagctacttg gatcagctgc tgtatttttg gttcattgtt
    12061 tccttcaaaa tgccagataa aagataaaaa ttctaagtat ttcctgttta gtttctccga
    12121 aaatgattca ggcatatctg gccgagattt tttgtttatc tttcttttta gagaacgcca
    12181 aactactcgg tagatggcga gataacgcgg cactttaagc caaatgatct tatctgcttc
    12241 tttaatcctt tcctctaacg tagaagtata atttccttca acgatccatg acggattaga
    12301 taccataaac gattgctgtt cttgcaaaaa ccactttttt gcctgaatgc ggaaagccgg
    12361 tgatttcttt gatcttcctt gcgagggacg attttcccga cccactttgt ccaatcacga
    12421 tgatcttcat tcttctccct cacttctctt tggaatttgg ttcgatcata ccacttataa
    12481 cgccgcaaaa aaa
//
LOCUS       DAZKOU010000084        12205 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000084 DAZKOU010000000
VERSION     DAZKOU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.085"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..331)
                     /locus_tag="ACG827_002268"
     CDS             complement(167..331)
                     /locus_tag="ACG827_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484389.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            626..1954
                     /locus_tag="ACG827_002269"
     CDS             626..1954
                     /locus_tag="ACG827_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0484390.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTVVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2156..2989
                     /locus_tag="ACG827_002270"
     CDS             2156..2989
                     /locus_tag="ACG827_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0484391.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3005..3742
                     /locus_tag="ACG827_002271"
     CDS             3005..3742
                     /locus_tag="ACG827_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0484392.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3897..4865)
                     /locus_tag="ACG827_002272"
     CDS             complement(3897..4865)
                     /locus_tag="ACG827_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0484393.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5097..5930
                     /locus_tag="ACG827_002273"
     CDS             5097..5930
                     /locus_tag="ACG827_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0484394.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5964..6803
                     /locus_tag="ACG827_002274"
     CDS             5964..6803
                     /locus_tag="ACG827_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0484395.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6829..7389
                     /locus_tag="ACG827_002275"
     CDS             6829..7389
                     /locus_tag="ACG827_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0484396.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7588..9309
                     /locus_tag="ACG827_002276"
     CDS             7588..9309
                     /locus_tag="ACG827_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0484397.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9474..10616
                     /locus_tag="ACG827_002277"
     CDS             9474..10616
                     /locus_tag="ACG827_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0484398.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10632..11843
                     /gene="thiI"
                     /locus_tag="ACG827_002278"
     CDS             10632..11843
                     /gene="thiI"
                     /locus_tag="ACG827_002278"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0484399.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4155 a         2049 c         2536 g         3465 t
ORIGIN      
        1 tgtcgtaggt tcgagtccta ctgccggagt aaataaagct tatgcaaaga aaaagaaatc
       61 caatcacttg ggtttctttt tcttttatta ttatgatgaa tcaagctttc tgtttttgtc
      121 acgtatgttg taatctttac gaaagagtag agttggctac tcataatcaa ccatacttcc
      181 catcatcgct aaatacgaaa taaatcagca ttcctacacc aatcaaagca caagctgcac
      241 caagaaaagg gatgacattc ccaaagttat ctgtattcat aagtatgcca ccacccacaa
      301 acaaaaagcc gccagcaatc aggtttttca ttttattcat ctcctttctt acgttatagt
      361 atttccttct ttcaatgagt attttgaaag ctatcttatt gtatgatgtt cttctaggcc
      421 tcttctttat tatacaggcc attttcgtta tctcatagaa atattccttt ttatctgaaa
      481 actattgtat accgtatgac atttttcttc atgaagttgt aagtatattc tcattcaaat
      541 gaaaatttca tcatagaaga gattgtattt ctaaattctt aagagtaaaa tacgaagtat
      601 aacttggatt tggaaggatg attggatgaa agctattacg cctgatatga caacaaaatt
      661 taacaaacag tttcaagcaa atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa
      721 cggaattact gcttctgctg aaaatgtttc agcacatgtg gagaatacac cggtattttc
      781 tatagatttg gctacgggga aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt
      841 tgctgcactg aacacattcc gtcataagat gctgaatcaa ttccatttaa aagagtttga
      901 attatcacaa aattacactt ttttctggga taaatatgaa aaggcaaatt atttctatga
      961 aaacatcatt gcaacaggaa aagaggcaat cgatagccgg aaagtagctt tcttactcgc
     1021 tactcctcaa caagatggcg gccaatggga tatgatcgtt tcattattcc aaaaatatgg
     1081 cgttgtgcct aaatcggtga tgccggaaag tgcgaatagt tcaaattctc gagatttgaa
     1141 taactacttg aacaaattac tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca
     1201 aggagaaaca ttagacgcta ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa
     1261 cttcttagct atctcattag gaacacctcc aaaagaattc gattttgagt accgcgatga
     1321 agaaaagaac tatcatcttg accgaggact gacaccacaa attttttatg ataaatacat
     1381 cggtgtaaaa ttagatgatt atgttagcgt gatcaatgca ccaacaaaag acaaaccatt
     1441 caatcgttct tatacagtag agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata
     1501 tctgaatgtt gatatggaaa ctttcaaaaa attagcgatt gctcagcttg aagaaggaga
     1561 atctgtttgg tttggttgtg atgtaggtca atcttctact agaactagtg gcatcatggc
     1621 cttagatgct tattcaatgg atgatctatt tgatacggat tttacaatga ctaaagctga
     1681 acgtttagat tttggagaaa gtctgatgac ccatgcgatg gtattgaccg ttgttgactt
     1741 agtagacggg caatcaacaa aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa
     1801 gaatggcttc tttgttatga gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt
     1861 aagaaaagat ttattgacga aagagcaatt aaatgcgttc aatgaagaac caattgttct
     1921 atctccgtgg gatccaatgg gtgctttagc atagacgagt atttcaatga agagtatgta
     1981 gtaaagataa aaagagctgg cttaagccag ctctttttat ctttaaagtc cattccctgt
     2041 tttttatggt ccctgtgcta taattctaaa taaaggataa ggattggaaa taatcctttt
     2101 atcattaaaa atatgatgac gaagtagaat aaaattggga gtagggggga aagtcatgga
     2161 tatccaaact gttttatctg atctgaaatt aaatggtaaa gtgattccag ttgttggtgg
     2221 cgatgttaat cagacctatc gtataaaaac cgaacatcgt gcttattttt taaaaatcca
     2281 tccgaatgta aaaaaaggat tttttgaagc agaagtagac ggattaaaag aactgtcagc
     2341 attcgtacgt gtaccggata cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt
     2401 aatggaatgg atcgagccgg gaaaagggga tcaacgagat cttgctgcag ctttagctaa
     2461 tctccatcag caaacagcgc cacaatttgg ttttcgtaaa gataactatt tgggtacact
     2521 tgttcagaaa aattcttttg aagaagattg gtggactttt ttctttaaag atcggcttga
     2581 atcccagatt tctttggcag aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta
     2641 tctgcgattt aaagaacgtg tattgaaatc ggttgaacca aaaaagatca ctccacgcct
     2701 cttgcacgga gacctgtgga gtggtaatgt gttctttgac cagcaagggc atccagtttt
     2761 tgtcgaccca gctgtttctt atgggaatcg ggaacaagat attgcaatga gccagttatt
     2821 cggtggtttc cgaccggaat ttttagatgc ttatcaaact atatttccat tagaaaaggg
     2881 atggaaagac cgactgccga tttaccagtt atattatctt ttggcacatt tgaatatgtt
     2941 tggagaaagt tacggttccc aagtagatca attactagaa aatttttaag aggagggcgt
     3001 gtttttggca aaaaaatata ccaaaaagca tgaagtttca tattatgagt gtgacatcaa
     3061 ccaaacgatg acatttcctt cgatgttagg agtagttatc aaaacgtctg aagatcaaag
     3121 cgaccagttg ggaagaggca gtgaattcgt caatagcttt ggacttactt gggtcatcac
     3181 gaattattcc atggagatta atcgtctccc tagagtcggt gaagtaatct cagtaacgac
     3241 tcaagccatg gagtacaata agtttttctg ttatcggaac ttctggatcc atgatgaaga
     3301 tgggaaagaa ttagtcaaaa tagaatctgt tttcgtattg atggattttg tgaatcgaaa
     3361 aatgagcagt gttaatgaag agatcattgc tccttttgag agtgagaaga tcaagaaaat
     3421 caagcgtcag gaaaaaattg aaaagattga ttcgggcttc atgttgccat atcgtgttcg
     3481 tttttatgat atcgatagta atcagcatgt taataatgcg atgtatttta attggatcat
     3541 tgacgtatta ggatatgact ttttgactac gcatgttcca gaaaaagtaa ttattcgatt
     3601 tgataaagaa gtagagtacg ggaatgagat cgaaagccat tttgaacaaa tccctatcga
     3661 aaatggtgtc aaaacaaaac atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa
     3721 tattatatgg aaaaataaat aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat
     3781 aagaagaaat ttacgaaaat tgtttctcaa atttttgtaa attttcgact tagttttcga
     3841 agaagctatt tttgtcacag cctctctttt taagtttagc tattagataa tgatgtttag
     3901 ttttggataa acgaaatcaa ttcttcaccc gacaaaccat agtttacagc aatcaatagt
     3961 tttaaacgtg ctttttgact attaatactg ttacagaaaa taatccccat tttttctagt
     4021 tcttttcccc cgccttcata atcataaaca ggctctgcga ttccattgaa acaacgagaa
     4081 acaagtacta cagggatttt ttggacaaga agcttttcca aagcagctaa ggcttgtgga
     4141 gggagattcc ccgcacctaa agcttcgatt accaaaccgt ctactttcgt atgggcgatg
     4201 gcttccaata agtccccttg catcccagca tatgctttaa cgatcgggat agtcccatct
     4261 acagcggaaa tgtccagtcg ctttgtttct aaaagttcct gcaagaataa aattcgattc
     4321 ttagttacta agccgatggg acccaatgta ggtgttcgga aagtagcaac atttgtggta
     4381 tgggtctttg tcacgtagcg tgcagaatgg atctcatcgt tcatgaccac caaaaccccc
     4441 ttatctagcg cttcttcaca acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg
     4501 tcacttccaa gttcattact tgaacgcatt gccccagtca aaacaattgg taatttgttg
     4561 ccaatcgttg tatcaagaaa aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc
     4621 accactccgg atatacctga tgcataagcg gacaagatac gttcttttaa taccaacatg
     4681 tgagaaggca tgatgtgtgg tgagggaaga ttgaagatat cttcaactaa taattctaca
     4741 tgcttgggga ttgtgatttc tgcatctaat aatgggtttg ccacatcagg actaacaccg
     4801 cccgtgagca tgttttcttt catggcgatc gttccgccgg tgtgtagtac aagtattttt
     4861 ttcattttac agacttcctt acgaataatt ttgcatagcc gttaaatctt attgtatact
     4921 atataaaaga gttttgctat cagtgaagaa taacgaatag cctaacttat cataaaaaag
     4981 ctgaccttat taaaatggaa aagactcaac cttgacctcc taatacaatg gagtatctgg
     5041 ttgagtttct tttacaagaa agcagccaag gaaatcaatc acgaaggtgg ccggaaatga
     5101 tcagaatcgg attaacatcg tttaaagaac atgacaaatt aactggtaaa aagaataaca
     5161 ctctttatga atatgcaggg tttctaccct tagtggaatt agatactgcc tattatggaa
     5221 ttccccgaaa gtcctcagtt gagaattgga taagtgaagt acctgaatca tttcgctttg
     5281 tagtcaaagt ctatcaagga atcagcggtc aaggagactg gaagccttat tatcagaccg
     5341 aagaagagat gattgcgcat tttttagatg ttatgtcgcc aatggtagat gctggaaaga
     5401 tgttttgctt tttagtccaa ttcccctcat cattcaaatg tacgaaagaa aatgtccagt
     5461 acttgcgcaa acttagaaag tggtttgagg gttatcctgt ttctatagaa ttgcgggatt
     5521 attcttggta cagcgatgaa tttcttgaac gtacaagaaa ctttatgtct agagccgctt
     5581 tttctttagc catcatcgat gagccacagt tgctgaatac aacgattcct tttgatccat
     5641 ttgtcaccaa tccgaacttt gctctttttc gattccatgg taggaatgcg caaggatggc
     5701 aagcaaatga taaagagtgg cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac
     5761 tgcaagaact agcacatgaa gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga
     5821 tttttaataa taattctggt ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat
     5881 taaatatcaa gccagatgga ctcaatccaa cccaaatgga tctgttttaa gggcttattt
     5941 tattcaagaa aggggcgaag tctatgatca aagctatttt ttttgatatt gacggaacat
     6001 tggtcaccaa tcgttcaaaa gcgttggaat caacaaaaca agcaattgaa tatgctcgga
     6061 aaaacggcat tttatgcggt gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca
     6121 ttgatgaatt ggaattagat atgtatgtcg tgtataatgg tcaactggtt tttactgcag
     6181 atcgcacgat tatcgaccac ccttttgaac aaaaagtatt ggaacatatt gttgaatttg
     6241 cagatgagaa ccatcgacaa atcgtttttg gtgcgagaaa tcggttagat ggatcaacga
     6301 cgatgctatt aggacaatcg atttttatca agcgactagt cagcttttta ccgcgtaaat
     6361 tcccggtacg tctgatgaaa aaaatcctcc aggtttttag ccctcaccga caaaaggatc
     6421 gttatgaaac gttagagata ttaaaagaac caatttatca atgtatattg ctcagtccgg
     6481 agtcagaaca gaaaaagtta gaacatcgct ttcccgagtg tacgtttcag cgttcaaata
     6541 cgtatactgt agatatcatc ccaaaaggtg gttcaaaatt actaggcatc caagctttcg
     6601 caaatgctgt cgaaattgaa atggaagaaa ttatggcatt cggcgatcat tacaatgata
     6661 tcgagatgct gaaaggtgta ggaattggag tggccatggg gaatgctcag atcgaagtaa
     6721 aacatgctgc agactttgtg actcaatcaa atgaagaaga tgggatatac gaaggactga
     6781 aacacttcgg tctgatttat tagtcaatga aagaaggaaa aaatcagtat ggaagcagag
     6841 acaccttttc aaaaagtaga aacattccac aaaacttttg atccaagaag accagagata
     6901 cccacggctt ttacagaaaa tgaagctcat acacgtgctg cgttcaaaat agaagaactt
     6961 gttgaatttg ttgcagctac ttcacctaat aaagaacaac tgcatgaagc tgttatgctg
     7021 ctgcacgaag cattagataa agcagaagaa aaaatacttg gtaaaacaca gtggggaagt
     7081 gatccgctgg ttgaacaagt agatgcactg acagatatcc tttactttac ttatggttct
     7141 ttttcattac taggaatagt gcctgatcga atattcgaaa tcgtccatca ggcaaatatg
     7201 gggaaattat ttccagatgg aaaacctcat tatgaccctg taacaaataa agtgttgaaa
     7261 cctgaaaact ggcagatgga atatgctcca gaacctaaaa tcgcggcaga aatccaacgt
     7321 cagattcaag aagcgattgc taagaagaaa tccgctgata taggcatgtg cacaaatgaa
     7381 aaagagtgat cagagtttca ctatttttgt tatctttata cgatggaaaa acaaaagatt
     7441 ttcgtttaag ttagtgaatg catgaaaata gtatgctata atctagaata gatgtctttt
     7501 gcacgagaga cgtattattt ggatttatgc caagcagaac gccatctgtt gcataaaaga
     7561 tgcatactga aaacggagga accataaatg ggaaataatg ttatcatcgg aatcgtcatt
     7621 gcaatcatta tagtcgccat tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac
     7681 caagatcgat tggatgcctt agaaaaacgc aaggaagaat tatttgactt gccagtgatt
     7741 gaagaagtcg atgatgttaa aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt
     7801 gaatggagtc aaaaatggaa tgacatatct acttcctcgt tcgctgaact agaaagtcaa
     7861 attttcgaag tggaaaacct caatgaaagt tttcggttct taaaaggaaa aaaagcagtt
     7921 gttgaagcag aagctacaat ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt
     7981 ctaaaggaat tgcgggaaac ggaagaacgc aactcactag ctgttcagca ggcacttgac
     8041 gtctatgaag aactgaaaga aaaagtcaga gaagacgagc atgaatacgg accagctctt
     8101 tttgaattgc aaaaacagat caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta
     8161 aacacttctg gagaccctgt tgaagcccgc gaggttttgg ataaagcaga aaaacatacg
     8221 tatgaagtgg aagctctgat gaaaaaaatt ccaccgctgt atgaggagct ccataccact
     8281 ttccctgaac aacttgaaga aatcagtgat acttacgaga aattgatgga agaacaatat
     8341 gtattcccag aagaagatct tgctgaagat atcgcaaaag taagtcgaag aattgaaaat
     8401 tcattagcca atcttgaaaa aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca
     8461 gctgatttga ttgattcgtt atatgatatc ttagaacggg aaatggaagc tcagagatat
     8521 gtaaaaacta accaatcaac gattgcagag tatatcaaac atacaacaaa gaataaccgt
     8581 caattattga ttgagctgga tcacacatct caaagctata cattgaacca caatgaaatt
     8641 ggccgtgtac gaggtttcca aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg
     8701 attccacaga tccgtcaaca cgaaattcct tattcagaag tgcgcacgtt ctataaaaca
     8761 gtcttcaaag tcttagaaga tatcgaaaag caacaagtag agatcgacga ctcattacat
     8821 gaattgagaa aaggcgaaaa agaagcgcaa gaaaagatcg atacattcga attcaaattg
     8881 cgcagtttga aacgttttgt agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta
     8941 gaattctttt ttgttgctac tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt
     9001 cgagtaaaca tggaagaggt caatcgttta gttgctctat gtgaagaaga tctagaatta
     9061 ttagataaga agacacatga tttagtagat gcagcagcat taacggagca aatgctccaa
     9121 tatgcaaatc gttatcgtca tacacacgaa gaagtgagag aagcaattga taaaagctac
     9181 tatttattca ataaagaata tcactatcaa gaagcattag acgaaatcgg tacagcatta
     9241 gagcgagtag aacctggtgc tttcaaacgc atcgaagatt tttattttaa tcatccagac
     9301 ttagtataaa gcaaaaaagt tagtaaacca gcaagagcaa gacggattat tgtcttgttc
     9361 ttgtcttttt ttgtatattt gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag
     9421 caccttggac aaccgttata atagaaaaga atttcaaaaa agaggggttt cttatgatat
     9481 attttgataa cagtgcaaca accccaatct ttccgcaaag cttagatgca tatgtaaaaa
     9541 ccagtcagcg gattattggg aatccatcaa gcttgcatga tttaggcaat caagctaacc
     9601 ggttgttgca gcaagcaaga aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa
     9661 tctattttac ttcaggcgga acagaaggag ataactgggt attaaaagga acgatgatcg
     9721 aaaaaagaga atatgggaat catatgatca tttcaggagt cgaacatccg gccgtcagcg
     9781 agacagcaga acaattaaaa gaattaggaa tcgaagtaag tatcgcccca gtggataaac
     9841 gcggatttgt acgtgtcgac gaattgaaag aattaataag aaaagagaca gtattagtct
     9901 ctgttatggc tgtgaataat gaagtaggat cgattcagcc catccaggaa atcagtgatc
     9961 ttttggatga ttttcctaaa attcatttcc atgtagatgc tgttcaggcg attggaaaag
    10021 taccattctc gcaatggttg acagaaagag ttgattttgc aacattttca gctcataaat
    10081 tccatggccc acggggaact ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat
    10141 tactgacagg tggagggcaa gaaagaaatc agcgaagtgg gacagaaaat gttccaggta
    10201 ttgtggctat ggttaaagct ttacgtttat cattagagat aaaggaacaa aaacctgagc
    10261 agacacaacg cttaaaagta gctctaatca atgcgttgaa gacgtatgag aaagtgacga
    10321 ttttttcaga aggaactgac tatgcaccgc atattttatg ttttgctcta aaaggaattc
    10381 gcggcgaagt tctcgtccat gccttagaag aaaaacaaat atttgtttct actactagtg
    10441 cctgctcctc acgtaaacat atggcaagca gtacattgca cgcaatgcat gtaccaaatg
    10501 atctggctac atctgctgtt cgaatcagct tagatgaaaa caattcgctt gcagaagtcg
    10561 agcaatttat gattattttc aagcagcttt ataagaaatt ttctgcaatc aattaaaaga
    10621 ggagtgttct tgtgaaatac agtgaaatta tggtccgtta tggggaactt tcgacaaaag
    10681 gaaagaatcg aaaaagcttt atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag
    10741 actttccaga aatcaaaatc catgctgatc gggaccggat gcatctttta ttgaatggcg
    10801 cagacgggca gctagttatt ccaaaactag caaaaatatt tgggatccaa aatttctctc
    10861 caagtattcg tgtagaaaaa gatgtcgaag ttttgaaaca agctgttcaa gacatcatga
    10921 aagaaattta tactgggaaa gaaacattta aaattatcgc taaaagaagc gatcaccaat
    10981 ttgaattgac aagtaatgaa ttgaatcaaa cattaggtaa tgctgttttt gatattttcc
    11041 cgcatatcca agttcaaatg cgtcaaccag atattccatt acgtgtagag attcggcgtg
    11101 atggcgctta tctatcgtat gaaacgatca agggagctgg aggattgccc gtaggaacaa
    11161 gcggaaaagg aatgcttatg ctttctgggg ggattgattc tcctgtagca gggtactttg
    11221 ccatgaagcg gggagtggaa attgaagctg ttcattttgc tagtccgccg tatacaagtg
    11281 aacaagcact gcaaaaagca aaagatctaa cagccaaact agcaccttat gtgggaacga
    11341 tccattttat cgaagtgcca tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac
    11401 aaggatattg gatgacaatt actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc
    11461 gtattcgtca tggtttagtc attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa
    11521 ctttgcaaag tatggtggct atcaatgaag taacgaatac gccaatcatt cggcctgtag
    11581 cgacaatgga taaacttgaa atcattgaag tagcagaaca gatcgataca tttaatcttt
    11641 ctatccagcc ttttgaagat tgctgtacga tttttgctcc tccacagcca aaaacacgcc
    11701 ctcaactgga aaaggtccat caatatgaag agcgtttagc aatagatgaa atgatccaac
    11761 gagcactagc tggcttgaag atggaagaga tacaagcaga atcgtctaat gaaaaaactg
    11821 aagaattttc agaattgcta taagaggtta tgaaaatagt gttttgacca gagtattaga
    11881 gaaacaaaac gaaaaaatag ctccatgtat tttaccgttt ttgaactaat gtcgaagatt
    11941 agctgattga ataccattta tcaagaagtt atggaaaagc cggttctttt ttactaactg
    12001 aaaaataaaa ttttatttag agatcaagct acagttgata aatgatataa aaaagagaat
    12061 ttcccatgaa atagcgaggt tgtgacagaa gtgggcagct tcaagaaata agaaggaatt
    12121 tacgaaaatt gcttttcaaa tttttgtgaa atttcggctt atttttcgaa gaagttactt
    12181 ctggaacacc gtgtatcaga ggttg
//
LOCUS       DAZKOU010000085        12167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000085 DAZKOU010000000
VERSION     DAZKOU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.086"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..310)
                     /locus_tag="ACG827_002279"
     CDS             complement(32..310)
                     /locus_tag="ACG827_002279"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484400.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(425..1042)
                     /locus_tag="ACG827_002280"
     CDS             complement(425..1042)
                     /locus_tag="ACG827_002280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484401.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1437..2261)
                     /locus_tag="ACG827_002281"
     CDS             complement(1437..2261)
                     /locus_tag="ACG827_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0484402.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2604..3020
                     /locus_tag="ACG827_002282"
     CDS             2604..3020
                     /locus_tag="ACG827_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0484403.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="ACG827_002283"
     CDS             3017..3487
                     /locus_tag="ACG827_002283"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0484404.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="ACG827_002284"
     CDS             3501..4274
                     /locus_tag="ACG827_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0484405.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="ACG827_002285"
     CDS             4288..5100
                     /locus_tag="ACG827_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0484406.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="ACG827_002286"
     CDS             5154..6119
                     /locus_tag="ACG827_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0484407.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6255..8978
                     /locus_tag="ACG827_002287"
     CDS             6255..8978
                     /locus_tag="ACG827_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0484408.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8991..9623
                     /locus_tag="ACG827_002288"
     CDS             8991..9623
                     /locus_tag="ACG827_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0484409.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9831..10055
                     /locus_tag="ACG827_002289"
     CDS             9831..10055
                     /locus_tag="ACG827_002289"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0484410.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10157..10390
                     /gene="relB"
                     /locus_tag="ACG827_002290"
     CDS             10157..10390
                     /gene="relB"
                     /locus_tag="ACG827_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0484411.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10380..10649
                     /locus_tag="ACG827_002291"
     CDS             10380..10649
                     /locus_tag="ACG827_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0484412.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(10828..12129)
                     /locus_tag="ACG827_002292"
     CDS             complement(10828..12129)
                     /locus_tag="ACG827_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0484413.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         3973 a         1973 c         2387 g         3834 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcagatgcag cgattaaatg actatcttta tcaactaaca ttgcttttga
     6301 aatctacgcc ttcagataat tattcagctc gcagtatttc gcaagctcta cagttgaata
     6361 gaagtacaat cagtagctac ttaaatgaag gtgttcgtga agggcttttc ataaaagtaa
     6421 aaagttaccc ggttttattt ttacatagaa cagctcttga agaactacat atcacactga
     6481 ataactccga aatagaatca attgaaagct tgttaacaat ttctcaaaaa cccgcattag
     6541 atcaagtaat tggctctaaa ggaagtttga aagaagcaat tgatcaaatt aaaacggcag
     6601 tcttatatcc tggaaaaggg ttgcctttat tattgatcgg cgctagtgga tcagggaaaa
     6661 cctttctagc gaataaaata tatgaatatg cagtagaaga aaaggtcatt cacacatcgg
     6721 cacctttcat agattataat tgtgcacaat atgtgagcaa tccagagtta ctatcctctg
     6781 ctttatttgg ctacacgaaa ggagcattta ccggagcaag ccaggagcat actggtttgc
     6841 tagaaaaagc cgatggagga gtattgtttt tagatgaagt gcatcgctta tctgaagaag
     6901 ggcaagagaa attatttaca tttatggata cgggtgaatt ctctcctatg ggggataata
     6961 gtattcggaa aaaagccgat gttcgattag tctttgcaac aacagaaaat atatatacga
     7021 ctttcttacc aacttttctt aggagattgc cagttattgt taatctacct agatttcaac
     7081 aacgtccgtc gtttgaacgt ctgtcattga ttgatgaatt ttttgtttct gagagccaaa
     7141 ttttagctaa ggaactatct gtttctgatg cattgattca tttcttaatg aatagtgatt
     7201 ttgaaggtaa cgtggggaat attaaaaata ttatcaagta tgcttgcgga aacgcatata
     7261 tccaccagaa gagtcagcaa acaattcaag ttaaattatt ggatatgcct ttagagtaca
     7321 gttataaatt caaggagcaa ctaaataaac ccagaaagaa aagagcagat cgaatctact
     7381 atccagaaga aacacgacaa gtgcaactag aaagaaacaa tcagcttttt gttcaatttt
     7441 ttgaccaatt acttactgga ttctctgaag tgattgaaaa taacaaggat attaaactat
     7501 ttttagaaga aatggttacc aaggtaacac aagtaatgga tacgcttatt tttcaagagg
     7561 attacgaaaa agagcagtct ttatacgcca ttctaaacta tcatattcgt caaagtattg
     7621 attttatgcg agaaagctac gggtttgaac aagatggtaa ccgggtagtc gctttggcca
     7681 actatctata tgtcaaggat aatcaagtga tttttgaaaa tagtcttcaa gaaaagaaaa
     7741 ccaaagatca gcttaacgaa ttttttgcta atgttcttga aaccccttat tggtatgcaa
     7801 aaaaaatctt aagcctttta tctgagtgtt tagatcaacc tgtgtatgaa gaggacattt
     7861 tctttattac gctatatttt tatagtttga atgtcacgaa tgttaaaaca ggtattaaga
     7921 gtattgtgct agctcatggt tattctacag ctagtagtat ggcgaatgtt gccaaccgta
     7981 tgctgaataa gaacgttttt caagcttatg atatgccttt agatattaca ttagagaaaa
     8041 ttgaagcaaa attgattcaa ttccttggag attatcgaac agattcaggc ttgattttat
     8101 tggtagatat gggttcacta aaccaactag gcggtttatt agtgaatcat ctaaatggac
     8161 cactgttaat cattgatcat atcaatacac cgttattatt ggaagtcggc caacaagtat
     8221 taaacaataa aagtatcacc gaaataggag cagaaattga aacaagtctt actttcaaaa
     8281 agcagttact acttcccaag aaaaagaaaa agaaagccat cattacttgt tgttatacag
     8341 ggattggtag tgcgacccag attcaggaga ttttaacgaa atgcttgaaa gatgcagtta
     8401 gagaattagt aattattccg tatgattaca aaaagctgtt agaaaacaag aattttgaga
     8461 ctccttttca attatacgat gtcatcatga ttgtaggtac agaagatccc aaaattaatc
     8521 agattccata tattggatta gatcgcctga ttaatggggg aagcattggc gaatttgttc
     8581 attacttgga aaaagagctg actattgaag tgaacgattt acaaaaagaa ttaattttca
     8641 atttttcaat tagtaaaatt gtcgaaaatt taaccatttt ggatccagat aaggtgctat
     8701 cttttgtaca aaaagcaatt aaagaattat cgaagttatt aaattttgat ttttctaata
     8761 atcagctgtt tttgttgtac cttcactgta gttgtatgat tgaacgaatt gtacgtaaag
     8821 aaagtgtgga tgagcaagag gacattgaag aatatatgca agcaaatgga gaaataatgg
     8881 ataaaattca tagcgctttt tttgagattg aaagggaata cacaattgaa ataccagatt
     8941 tagagttgcg attattgagc gatattgttc aaggatagga gtgaattcga atgacaatgg
     9001 gagtaatttt tgatatggat ggggtactca tagatagcga gacattttat tttgagagaa
     9061 gaatgaactt ctttcaagaa ctggagatag atccaggatc aaaccagatt gatgattatg
     9121 tggggaaaac agaagctgca atatgggaaa cattagttcc tgaggacaaa tcattaagag
     9181 aggaacttta tccgaaatat gtagattatc gaaaaacgca tccaattgat ttcgctaaag
     9241 ctttacgaaa agaagtgaaa ccgttacttg acttcctcaa gaagcgggat ataccattgg
     9301 cactggcatc ttcttctcca gctgtcgaga tagagagtat gctcattcaa tgtgaactta
     9361 ctgaatattt ttcctttgtt ataagtggtg agtcattaaa acaaagcaaa ccacatcccg
     9421 aaatatattt aaaagcgaaa cagcaattag actgtgactt ctttattgca gtagaggatt
     9481 caacgattgg tattaaatca gcaaaggcag caaatatcta tacggcggca ctaaaacaac
     9541 caatgcaaat cgatcagagt gaagcagaca tacaaattga aagtctacaa gaactggaac
     9601 aaattgtttt agaaaaaggt tagtgaatta atggaaataa ggctatatat ctgtgctagc
     9661 tattttgact aaccaaaaaa acttgctata attcaggtgt agtaaaaggt gctggtaaca
     9721 ccttttacga aaatgcttcg gcagtgataa tgggattggc tttttaagtt ttttattttg
     9781 ttcgcaatcc ctaaggtcat gtgaatgatc ctagggattg ctagaatatt ttgcaacaga
     9841 accaaaatac gaatagttat caagcagcca tattgactgc ggtcaattta ggaggggata
     9901 ctgatactac tgctgccgta actggtgctc ttgcgggtat tctttacggg ggtgaagtga
     9961 tccctgaaaa ttggagaaaa tcgcttcaaa atgaccagct tcttaaaacg atcagtcaga
    10021 gattttgcga taccgtgcta tctcaaagaa tttgaataat ggatatgatg caaatcttgt
    10081 ttgtactaac gttgtattga caaaaaggat tttatttagt acaatgttag tacaaatgat
    10141 gaacggaggt agaatcatgt ctcaatcaac cattacattt aggattcccg acgatgaaaa
    10201 agaactggta ttagaatatg caaaagcaca caattcttca ttgaccgaac tttatcgaaa
    10261 tgctgtactg gataaaatcg aagatgaaat cgatctacgg actttacgga gtgcaattga
    10321 agtttccaaa gagaaaaaag agagtggtat ttctcaagag gaaatggaga aactgctaaa
    10381 tgaattataa agtcatctac atgccgcaag cccagaagca attaaaaaaa atggacagaa
    10441 accaagctaa aataattgtc gcctggatca gaaagaattt agaaaacacg actgacccgc
    10501 gtcagcacgg aaaaggtttg actggaaata agagtgggga atggcgctat cgaattggaa
    10561 actatcgaat attggctaat atttgtgatt ctgaaattga aatccagata ttctcaattg
    10621 gacatagaag tacaatatat aaaagataac cgtttttgga acgaagtaga gtttcgaaag
    10681 tcaatgtata atcttttgaa ttttgcataa taccacaggt agaaactacc cgatactatc
    10741 ggttggtttc tgctttttta tggctctttg tcaaatagga ctgatagagt gagataagaa
    10801 catctaaggt agaattcctt ttctacttta gatgtttttt gattcatagg taatcagatt
    10861 atttagtaag aaaattctgg tcttcatttt ccgaaagctt cgaaagccat aagcgactcg
    10921 tttcaatgct ttgatgtggg tatgcagatt ttctattttg gcatttgaat aaggcaattc
    10981 gagggctaaa cgaatgccgg attcatagtt tataaaagct ttttttatag aatgcagttc
    11041 ttttggaacg gaattgggca tcgttcttac aagtttaaag aaagtgtccg cctctttgtc
    11101 gtgaaacgct tgaagaattt cttgatagta aaaataggcg attcgtaatt cgtcagagat
    11161 cgaaagcaga cgatccacca cttcactttc ggtgagatac gcccaaccaa aattgcgcca
    11221 tttggtccat ttgtcataaa caagcttgtc ctgtcgtttg atcagaagcc gagacaacgc
    11281 ctttaattgt cgataatatt tggcttgtgt tggatcgttt cgatcaaagc ttttcatgat
    11341 tcgtacgcga atatcctcaa atttacgaat gagatgtttg acaatgtgaa aacgatccac
    11401 aacgagtttt gcctttggaa aacattcctt tatcagagag gcataagaaa cattcatgtc
    11461 cgtcacgatg acctttacct tcaatcgtgc gcgtcgttca aattgcataa aataagaaac
    11521 gagagccttt ttcttccgtg aaggaagaat atccagtaat tttccagtat cgccatccat
    11581 acaagaaaag gagtagggac cgacttgatt tttcagtgaa cgaaattcat ccattaataa
    11641 acaacgcggt aaccaatttc tttttacttt tgtcatgggg atcagagaag cgagtgtccg
    11701 agaaacggtc gaccaagaaa cgccgcaatg cttagcaatt gtcgtcattg cttgtttttc
    11761 agttagctct tctaaaattt tatagcgcac ttgtttagcg ataaagcagt ttctatcgac
    11821 aatgtaggta ttcgccgtaa aataggtgtg gcagccacga cagtggtagc gttgtttcgc
    11881 taaagcaaga taagtcagtt ggttcgcaca tttattaagt tgaatgagcg attttttctt
    11941 tccgttctta acgatcatgc cttcatgatt catctgttta cacaaaggac agcgacgaat
    12001 ttctttactt aacgtcgcag aaacaacaag actcttgata ttcttaatgc tcttcgattc
    12061 cacatttgtg atgaaaatgt ttttatcttt gattccaagt agtagtttga tatgatgaag
    12121 atcaggcata cagaacgctc ctttgattgt gttgtggtga ttcaatt
//
LOCUS       DAZKOU010000086        12154 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000086 DAZKOU010000000
VERSION     DAZKOU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.087"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..593)
                     /gene="frr"
                     /locus_tag="ACG827_002293"
     CDS             complement(36..593)
                     /gene="frr"
                     /locus_tag="ACG827_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0484414.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(596..1318)
                     /gene="pyrH"
                     /locus_tag="ACG827_002294"
     CDS             complement(596..1318)
                     /gene="pyrH"
                     /locus_tag="ACG827_002294"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0484415.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1454..2335)
                     /gene="tsf"
                     /locus_tag="ACG827_002295"
     CDS             complement(1454..2335)
                     /gene="tsf"
                     /locus_tag="ACG827_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0484416.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2434..3216)
                     /gene="rpsB"
                     /locus_tag="ACG827_002296"
     CDS             complement(2434..3216)
                     /gene="rpsB"
                     /locus_tag="ACG827_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0484417.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3575..4054
                     /locus_tag="ACG827_002297"
     CDS             3575..4054
                     /locus_tag="ACG827_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0484418.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4271..5362
                     /gene="phnW"
                     /locus_tag="ACG827_002298"
     CDS             4271..5362
                     /gene="phnW"
                     /locus_tag="ACG827_002298"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0484419.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5355..5483
                     /locus_tag="ACG827_002299"
     CDS             5355..5483
                     /locus_tag="ACG827_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0484420.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5487..6032
                     /locus_tag="ACG827_002300"
     CDS             5487..6032
                     /locus_tag="ACG827_002300"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0484421.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6486..8177)
                     /gene="argS"
                     /locus_tag="ACG827_002301"
     CDS             complement(6486..8177)
                     /gene="argS"
                     /locus_tag="ACG827_002301"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0484422.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8597..9544)
                     /gene="arcC"
                     /locus_tag="ACG827_002302"
     CDS             complement(8597..9544)
                     /gene="arcC"
                     /locus_tag="ACG827_002302"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0484423.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9659..10678)
                     /gene="argF"
                     /locus_tag="ACG827_002303"
     CDS             complement(9659..10678)
                     /gene="argF"
                     /locus_tag="ACG827_002303"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0484424.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10769..11998)
                     /gene="arcA"
                     /locus_tag="ACG827_002304"
     CDS             complement(10769..11998)
                     /gene="arcA"
                     /locus_tag="ACG827_002304"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0484425.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3317 a         2528 c         2199 g         4110 t
ORIGIN      
        1 gcggaatgat ccgcccctct gtttttatga ttttattaaa cttccaataa ttctttttct
       61 ttatcagcag cgatcttatc tacttctttg atgctgttat ctgtcaatgt ttgtacgtct
      121 ttttctaatc cgcgcaaatc atcttcggta atatcaccat ttttttgttg ttttttatat
      181 tcatcgatcg catcacgacg gatgttacga atcgcgattt ttgcattttc tgcttctttt
      241 ttcacatctt tagctaattc ttttctgcgt tcttctgtca gttgcgggat aactaagcga
      301 atgacatttc catcattggt tggactgatt ccgatatcac ttgccataat agctttttca
      361 acatcttgaa gaatgctctt gtcaaaaggt gtgatcatta aaacacgtgc ttctggaatt
      421 tggattgatg ccatttggtt caatggtgtt ggcgcaccgt agtagttcac agtaatacgg
      481 tccaaaaggc tagcgttcgc acggcctgcg cggatttgcc caagttcacg ttgaagattt
      541 tgtgctgctt ttttcatttt ttcttttgct tctgtcataa ttgcatctgc catttttatt
      601 tcccccttac agttgttccg atattttcgc ctaagattgc acggcgaata tttccgtgtt
      661 catttaaatt gaagacaagc agtggaatat cattatccat gcttagtgaa cttgcagttg
      721 aatccatgac ttgtaatcct ttggcaataa cttccagatg agtcagttct tcaaatttta
      781 ctgcatttgc atctactttc ggatcagcag aataaacacc gtctacattg tttttagcca
      841 tcaggataac atctgcgccg atttctgccg cgcgtaatgc tgctgttgtg tctgtcgaga
      901 agtacgggtt gccggtaccg cctgcaaaga taacgatccg tcctttttct aaatgacgtt
      961 ctgctttacg gcgaatatat ggctctgcga tttgacgcat ttcaatcgaa gtttgcacgc
     1021 gtgtcggtac acccacgttt tccaacgtat cctgtaaagc taaggcattc ataactgtag
     1081 ctaacatacc catgtaatca gcttgagcac gttccatgcc catttgtgct ccgatttgtc
     1141 cgcgccagat atttccgccg cccacaacga ttgccatttc aacgcccagc tcgtgaactt
     1201 ctttgatttc ttggatgatt tctttgatta ctggtggttt gattccaaat cctgcgtccc
     1261 cagctaaagc ttctccactc aattttaaga ctacacgctg atatttcggt gtgaccatgt
     1321 tcattcctcc aatattttct attgccattt taccatactt agatgatttc gacactctaa
     1381 gactggtttt tcttgttttg cttaaaaaaa gggagcgcac aaaccgtgcg ttcccgtctg
     1441 ttattcaaca gaattatttt ttcacttggc tcataacttc ttctacaaag ttatcttcgc
     1501 gtttttcgat accttcgcca acttcgaaac gtacgaatgt tttaacagtt gcgccttttg
     1561 atgcaacgta tttttcaaca gtcatatcag gatctttaac gaatggttgg tccaccaaag
     1621 cgatttctgc tttgaatttc ttcaagcgtc cttctaccat tttttcaacg atattcgctg
     1681 gtttgccttc gttcaatgct tgttctgtaa gaacagtttt ttcatgttct aattcagctt
     1741 ctggaatttg tgattcgttt acataacgag gattgatagc tgctacgtgc atagctacgt
     1801 ctcttgcaac tgtttcgtca gttgttccat ctagaacagt caatacggca atacgtccgc
     1861 ccatatgcaa gtatccacca aatgcagcat tgtcgtcttt ttctacaact tcaaagcgac
     1921 ggaagctgat tttttctccg ataacttgag ttgcttcgat caagtcagat tcaattgtac
     1981 ctttatctgt tttgattttc attgcagctt ccatgtctgc tggtttgttt tcagcaacaa
     2041 gttctgcaat ttcttttact aaatcttgga acatttcgtt tttagaaaca aagtctgttt
     2101 ctgagtttac ttcaacgatt gctgcagtgt tacctttcac agcaactgaa gccaatcctt
     2161 cagctgcaat gcggtcattt ttcttcgcag cctttgccat tcctttttca cgtaaaagat
     2221 caactgcttt ttccatgtcg ccttctactt caactaatgc ttttttcgca tccatcattc
     2281 ctacgccagt catttcgcgt agttctttta ccattttagc tgtaacgtct gccatgttta
     2341 actcctcctg tgtgtttttc tttttaaaaa aagctgcttc aaaggagagt gaggcttgtc
     2401 gcccagcctt tgaaacagct ccatcattca aaattattct gctgaagaat tgtcgccttc
     2461 aacaacatct acgatttctt cgattgatgt tgctgtgttt tcttctgtga aatcttcttc
     2521 aactacttga tcttcacctt ggtttccttc gatgaaagca tcagccattt ttgaagtaat
     2581 caatttaacg gcacgaattg catcgtcgtt tgaagggatt actacgtcga tttcatctgg
     2641 atcgcagttt gtatcaacca tagctacgat tgggatattt aatttgtgtg cttcttgaac
     2701 agcaatacgt tctttacgtg ggtcaacgat gtacattaca tctgggattc ttggcatatc
     2761 agcgataccg cctaagaatt tttcaagacg ttcacgttct ttgttcaaac cagcaacttc
     2821 ttttttaggt agaacttcaa aagttccatc ttcttccatt gcattgattt gtttcaaacg
     2881 gctgatacgt ttttggatag tatcccagtt tgtcaatgtt ccgcctaacc aacgatggtt
     2941 aacgaagtat tgtcctgcac gagttgcttc ttctttgatt gcttcttgtg cttgtttttt
     3001 tgtacctacg aataatgcta cgccgccttc ttcagcaacg ctcttcatgt aatcataagc
     3061 tgcatctact aatttaactg ttttttgcaa gtcaatgatg tagattccgt ttctttctgt
     3121 gaagatatat ttcttcattt ttggattcca gcgacgagtt tggtgaccaa agtgtacgcc
     3181 ggcttctagt aattgtttca tagaaattac tgccatgttt gtttcctccg atttggtttt
     3241 atttttcctc ttcttatctt ctaccttgcc ggcaaacttt ttcaagcacc taccgacaat
     3301 ccaataagaa tgtggatttg ctgatgcata aagcaccctc agctattata cagacaaaag
     3361 atccgtatcg caagagcaaa tatacttttc tgctcaacat tcaatagttt acctgaaaga
     3421 taaaaagaat tcaagaaaaa cagtagattt ctctttgttt ctttattatc ctttttccat
     3481 aaaataattt taggtataaa agacgaatat tattgggcaa tccttctttt ttttgttatg
     3541 atacatgtga aatctttcgt aaaggagaaa gaaaatgcgc aaagctcaaa gacatttgct
     3601 gattaaacaa gtcattgaag aattcccgat ccgtacgcaa gaagaactat taaacaaact
     3661 tgggcattat ggcgttaccg ccacgcaggc aacgatttct cgagatatcc gtgatctaaa
     3721 aatcgtgaag gcacctgatg aaaatggcgt ttcccgcttc gtattgtttc gcgaaaaagg
     3781 aactcctgag actaggactg aggaagagga tcggctggtt caaatgattg aagacattgt
     3841 attgaaagtc gaacgagtcc actttttgac gatcgtcaat accttgccag acaatgctca
     3901 tttatttgca tctgttttag atgagatcaa acctccctct attgtcagta caattgctgg
     3961 attcgatacg atcatggtca tctcggaaaa tgaggaggac gcaaaacgag tagaagaatt
     4021 tcttcaccat ccaactgaag tatcactttt ttaatcagag cacgtattcc taatatggaa
     4081 tgcgtgtttt tttattttca atattttcct tattgaataa acctttacac tctttacaaa
     4141 aatacatcat tttttattca aatttttcaa caccttgatt gaatcacaat aatcatcatt
     4201 tatattggat gttaacaaga gcagcttctt gctctttgtg tatttcaaaa aagaaatgaa
     4261 aggaaatcat atgtataaat tattaacttc tggaccattg acgacttctg aaacagtgaa
     4321 aaatgctatg ctgatcgatc attgtacttg ggatcaagaa tacaaagaaa ttacacaatg
     4381 gataagaaaa aaattattgg atcttgctca agtatcggaa ggaatataca caacggtctt
     4441 aatgcaaggt agcagctcct tcggggtaga atcggtatta agttctgtga tcaggccaga
     4501 agatactgta ctgatttgtt caaacggcgc atatagacaa cgaatgatca acatgtgcga
     4561 atgtctgaaa ctgaactata ccgtttatga ggtatcggaa catcaaatcc tccaagctca
     4621 tgtgattaca cagctgattg aagagaatcc gtctatctcc gcactagcaa tggtccatag
     4681 cgagactact tccggtattt tgaataattt ggaagagatt gctgctgtca cacaagaaag
     4741 agatcttttg tttatagtgg acgcgatgtc aagctttggt gggataccga tttgtgtagc
     4801 cgaactaggg attgattttt tgatttccag ctcaaacaaa tgtattcaag gtgttccagg
     4861 cttttctttt gtacttgcta ataaagcgga actggaaaaa cgaaaaaatg ttagccgaag
     4921 tttgtcactt gatctatatg accagtaccg cgtgatggaa aacgagaatg gcaaatggcg
     4981 tttcacttcc cctacccata cggttttggc ttttcaacaa gcgctgaagg aactagaggc
     5041 agaaggagga atcgaaactc gatattcacg ttatctcgcc aataatcatg tgctacgaca
     5101 aaaaatgaca gagatggggt tccagccttt cattcaagaa tatcaggggc cattcatcac
     5161 ctcttttctt taccctaagg actggcgatt tgattttaat catttttatc agtatttaaa
     5221 gcaaaatgga tttgtgatct atcccgggaa aatcagcgat catgaatgtt ttcgtatcgg
     5281 aaatattggc gagatctatc cggaagatat tttggaactg acagcaatta tcaaagacta
     5341 taaggagaac gttaatgatt aaaactgtaa tttttgattg ggcaggaaca actgtggact
     5401 ttggctgtat ggctcctgtc cacgctttta gaaatgcttt tctagaaaaa ggaacccaac
     5461 tgactgacaa agaaatccgc taaccgatgg gaaaattaaa atgggatcat atccaacagc
     5521 ttctttccct cccttctgtc aaaaaacaat ggatagctat ttatggacaa ctacctcagg
     5581 aaaaagaggt ggaggaattg tatcaacaat ttgaacatta tctgtttaaa gatttggtca
     5641 agcattctac cttgaaaccc gacacattgg aaacgatcga acggttgaga aacgctggga
     5701 tcaatattgg ttccacgaat ggatttaatg ctcctatgat gacaatcgta gcagaaaccg
     5761 caaaaaaaga aaggtattca ccagacttca tggcaacttt tgaagacgtc gaagggtatg
     5821 gtcgaccgtt tccttatatg atccataaaa acatgcagta cttcaaaaac aagtcggttg
     5881 atgaagtaat caaagtcgga gatacggtct cagatatcca ggaagggaaa aatgccggag
     5941 tgttgacagt aggtgtaatt gaaggtagtt cgctaatggg gatttcttat gatgagtacc
     6001 aaaaattaga gatggggaaa aagatcgttt aaaacagtct gttgctctta gattccaaca
     6061 agccggcgct gatatttgtg taaacaattt aaaagagtta acagaataca ttttgtacgc
     6121 tgacaagaat ccagaatgac ttttatacaa aataagaggt tgtgaaaaag ctgtcctgta
     6181 tcaagtaata aaaacagcca agcaaaaata gctcctcata tttttcgttt ggttaggctt
     6241 attaccgcag atgcaagctt ttgaacaccg tttattcgaa aaaaaaaagg gagctgtgac
     6301 acgacttttg tcacagtccc cttatttgca aacaaacagt gtttaagaga caacctctcc
     6361 actaaacggt gttcattcag ctgactctcg gtatttgctc aaaaaacgat aaaatttgaa
     6421 aagctatttt ctcttttgtt tctctaatac tcggatcaga acgctgattt cacattctct
     6481 ttttcttaca tcttatctgg tgcatgaagt cctagcaaac gaaggtcttc tttcaataat
     6541 acagtcactg cataaactag agctaagcgt gcttcttttt gttcatcatc agctaaaatc
     6601 ttcgtatgag catagtattt gttgaatgct tgtgctaatt tgattgcatg tttcgcaatc
     6661 actgacggtt cgtatttttc cccagcagac aagaccgttt cagggtattt ttgaactaat
     6721 ttaaccactt cccagctggt atcgtcattc aatgcatagt ctgctgtttc tgaagggaca
     6781 aatcctgctt tttccaacag gctgactgca cgagcatgtg tatattgcac ataaggtcca
     6841 gtttctcctt caaatcgaac aacttcttcc aggttaaagt caaatgtatt cagtcgatcg
     6901 tttttcaaat cgtggaaaat gactgcccca accccaactt gtttagctac agcatctttg
     6961 ttttccaaat cagggttttt ctcgctgatt tgttctttag cagattggat tgcttcattt
     7021 aatacttctt ctaataagac gatctttcct ttacgagttg ataatttttt accaccttgc
     7081 gtgatcaaac caaatggaat atggtgcatg tcgtcagacc agtcaaagcc catttccttc
     7141 aagacagctt tcaactgttt aaaatgatag ctttgttcat tccctactac atatagagat
     7201 tgtttgaagt cgtaagtacg tttacggtac aatgcagcag ctaagtcacg tgtaatgtat
     7261 agtgtcgcac cgtctgactt cttaatcaac gctggattca ggtcataagc agataggtca
     7321 acgatctctg cacctttatc ctcgttcagc aaatgttttt cttctaaaag tttgacgatt
     7381 tcatccatct tgtcattgta gaaagcttca ccgttcaacg aatcgaaacg tacttccagc
     7441 agatcataga ttttattgaa ttctttcatc gattcatcac ggaaccattg ccataattga
     7501 atagcctctt tatcgccttc ttccagacgt ttgaaccaag cacgagcttc ttcatttaat
     7561 tctggttctg tttcagctac ttcatggaat tggacgtata aacgaagcaa ttcattgatt
     7621 ggttctgctt taacagcttc ttctgtaccc cattttttgt aagcgacgat cagttttcca
     7681 aactgtgttc cccagtcacc taaatggttg atgcggattg gctgataacc gattttttcc
     7741 ataatgaaac cgatcgaatt accgataact gtcgaacgca aatgtcccat tgagattggt
     7801 ttggcaatat taggagaaga catgtcgatt ggtactgttc cttgattccc gatgttgcta
     7861 tccccataat gttctttttc tttcactact gtttgcaaaa cttttttgct gataagttct
     7921 ttattcatga aaaagttcaa gtaaggtccg acaacttcga ttttttcaaa gttggcagaa
     7981 tcgatttttt ctgctaaatc tgcagcgatt tgctgtggcg cttttcggta aacttttgct
     8041 aatgaaaagg ctggaaatgc aacatctccg tgttcagctg attttggatt ttctagtaat
     8101 tgctcgactt gttccagtgt taaatcatct ttgacagcgt catagacagc ttttgcgaca
     8161 agatctttat tattcatctt tattcctcct aaagtgttcc cttgattgtt aaacaaaaaa
     8221 aatctctagc gacggcatac ttagtatgcc atcactagag acgagatttc ccgcggtacc
     8281 actccaattg tcctaaaaaa ggaccagctc catctgatat cggtagacga ccgtctgttt
     8341 aaaacagaat tctccaagtg cgcttcgttg tatcttcttc attcggcttc caccacttcc
     8401 gaattcgcta gccaagaatg atacaagtac tctcttgttc aacaaatctt agtcttatta
     8461 aactttcttg aattatatca gaaaaaaaaa cgaaaggcta gtcgtttcat aaaaaaaaaa
     8521 tccagatggc ctaaaggaga agaaatacca cctggatagc tataaatcat ttcgtaaata
     8581 cgaaagactt aacaaattaa tcagctacga taatcgttcc ttcttcagaa gctaaaagat
     8641 tctcgatatt ttctaaagaa gtgatgattg ccttagcgtt tggttttgct tcaacaaatg
     8701 caatagctgc ttctactttc ggaagcatgc ttcctggagc gaattgcttt tcatcaatgt
     8761 attgtttcat ttctgaaaca gtaacagttt ctaatttctt ttgatctggt ttttggtaat
     8821 tgacatacac attgtctact cctgtcaata cgatcagcaa atccgcatcg atgatttcgg
     8881 ctaatttttc tgaagcaaaa tctttatcga ttacggcttc tcgtccttct aatccagtag
     8941 ctgtttctac aacaggaatc ccaccgccac caacagagac tgtgataact ccttgatcga
     9001 ccagcgtttc gatgacacgt gcttctttaa tcgagatggg tttaggggaa gcgactactt
     9061 ttctccatcc acgtccagca tcttctttga atgtcacagt ggagtcagca ttcatttgtt
     9121 ctttggcttc ttcttccgtg tagaatggac cgactggttt acttggattt ttgaatgaag
     9181 gatcattttc atccacaata acttgagtga ctaaagaaac cacatcttta tcgatccctt
     9241 tttccttcag tacttcgccc atggcgtttt gtaaccagta accgatagat ccttctgtca
     9301 ttgcaacaca tgtatctaaa ggcattgccg gtgttttctc agaatcagca gcttgctgct
     9361 gaatcaatag gttaccgact tgcggaccat ttccgtggga gatgatcaat tcatctcctt
     9421 gttcgataaa tttcactaaa tactttgctg tttccattaa cgcttcttgt tgggcttttg
     9481 cacttgcatc cgtagacaag atggcattgc cccctaaagc gacaactact tttcgtttgg
     9541 ccatttcatt cgtcctttct tcagttctac cgaattttct agataacaaa acgagccagc
     9601 gttttgtgat tttccctcaa tgctgactcg ttttttcgtg agatgaatgt ggttatgctt
     9661 aaacgcgagg gataaacaag ttacctaatg ttgctgccat gatcgctttg attgagtgca
     9721 tacgattttc tgcttggtca aattgacgag catatttgct gcggaagact tcatctgtta
     9781 cttccatttc agtgatgcca aagcgttctt tcatttgttc accgtaaaca gtgtttgtat
     9841 catggaaagc tggcaagcag tgcaagaaga tcaaacgatc tgtgttgtgt gttttttcta
     9901 ccatttccat attgatttgg taaggtttca acaatttgat acgttcttcg aatttatctt
     9961 cttcacccat tgatacccat acatcagaat aaaggacgtc tgctccgtct acacctttat
    10021 caacatcgtc ggtaatcatc aattgtgcac cggatttttc agcaaagcct tctgccattt
    10081 tgacgatttc ttcttcaggt tgtaattctt ttggtgcaac gatacgcata ttcgctccta
    10141 aaattgcacc tgtgactaat aaagagttag ccatattgtt tcgaccatct ccgcagtaag
    10201 caacagtgat accttctact gttccaaagt tttcttggat agttaaaaag tcagcgatca
    10261 tttgtgttgg atgccattca tcagtcaagc cattccaaac tggaacacca gagaattctg
    10321 ctagttcctc taccattttt tgactgaatc cacggaattc gattccgtca aacatacgtc
    10381 ctagtacttt agctgtatct tctgtggatt cttttttacc taattggata tcatttgcac
    10441 ctagatattc aggatgtgca cctagatcga tcgctgcagt agtaaatgca gaacgagtac
    10501 gtgtagatgt tttttcaaat aaaagcgcaa tattttttcc ttctagatag tgatgtggga
    10561 taccacgttt tttcaagtct ttcaagtgtt ctgaaaaatc gatcaaatat tgtaattctt
    10621 cttttgtaaa gtctttttct gctaataagc ttcttccttg gaatactgat tctttcatat
    10681 taaacattct cctttagatt gtttttattt aagattaaag aggaaatccc gacggcagct
    10741 gacagtaaga ctgccgtcag gaaacaaatt acttcaagtc ttcacggaca agtggttggc
    10801 tcatgcagcg tggaccacca cgacctcgtg acaattcact tgaattgatt tctaacactt
    10861 tgatgccata gctacgaagc aattcgttcg atacataatt tcggttataa gtgactacaa
    10921 cacctggagc gattgccaat gtgtttgatc catcattcca ttgttctcga ggtgcaacga
    10981 tctcatctcc attaccagtt gggatcagta caagatcatc taatccaaga gcagctttca
    11041 tcgtttcgtg taagttatct gaatgagtaa tcttgatatc atcaccatct ggttcgattg
    11101 taaagacatc catcttaccg tctttacttt gaattgctgg atggatagtg aatttatcgt
    11161 gatctaccat tgtaaatacg gtatccaaat gcatcattgc acggttgttc ggaattttga
    11221 ttgccaacac tttttcaaat cctgaatttt ttgcgaatag gtggcgtgct aatttttcta
    11281 aagctttcgc attagttcgt tgagaaatcc caacagctac tactttatcg cttaagatca
    11341 attcgtctcc gccttcgata tgatctggat tttcacgatc caaccatact tcgattcctt
    11401 tatttgcaaa tcttggatga tgtttcaaaa tatattctgt aaacatcgat tcacgacgac
    11461 gtgcttcaaa agtcatctta ttgactgtca tcccatttcc cattgatgcg gaagggtcgc
    11521 gtgtaaagta caagtttggc attgggtcca tgtaaaatgg atagtcatcg tctgctgaca
    11581 gatcatatag actgctagaa cgtacttcga tatcttttgt acgaacccca gccatgattt
    11641 tatctaccat ttcttgcgtt tccattgata gaaggaactc atgaagaccc tctcttacag
    11701 cattcgatgc aatatgagat tcatccaaca ttttattcaa gaattgttct ttgacattgc
    11761 ctgcgtcaat cgcttctgcc gctaattttt ccaagtaaag agtctcgacg ccttcatttt
    11821 gcaatgtctt cgcaaagttg tcatgttctt cttgagcaat cggaagatat ggaatatcat
    11881 cgaacaacag acgatccata atatctggtg tcaaattttc cacttcttgt ccaggccgtt
    11941 tcagtaaaac tgtctttaat ttccctattt cagagaaaac gtgaataggt ttatccatgt
    12001 tgtgttgcct ccttcttttt gattacatgt ataggatatc ctccttttgt aatcactttc
    12061 ataattttgt ctcattcaaa atgagacatt cctcacatta atattcgata attaaataat
    12121 taaacgtttt tctattttta agcgtaattt attc
//
LOCUS       DAZKOU010000087        12127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000087 DAZKOU010000000
VERSION     DAZKOU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.088"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(249..2324)
                     /gene="glyS"
                     /locus_tag="ACG827_002305"
     CDS             complement(249..2324)
                     /gene="glyS"
                     /locus_tag="ACG827_002305"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0484426.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2326..3243)
                     /gene="glyQ"
                     /locus_tag="ACG827_002306"
     CDS             complement(2326..3243)
                     /gene="glyQ"
                     /locus_tag="ACG827_002306"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0484427.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3629..4447)
                     /gene="recO"
                     /locus_tag="ACG827_002307"
     CDS             complement(3629..4447)
                     /gene="recO"
                     /locus_tag="ACG827_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0484428.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4590..5489)
                     /gene="era"
                     /locus_tag="ACG827_002308"
     CDS             complement(4590..5489)
                     /gene="era"
                     /locus_tag="ACG827_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0484429.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5504..5899)
                     /locus_tag="ACG827_002309"
     CDS             complement(5504..5899)
                     /locus_tag="ACG827_002309"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0484430.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5883..6359)
                     /gene="ybeY"
                     /locus_tag="ACG827_002310"
     CDS             complement(5883..6359)
                     /gene="ybeY"
                     /locus_tag="ACG827_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0484431.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6388..8580)
                     /locus_tag="ACG827_002311"
     CDS             complement(6388..8580)
                     /locus_tag="ACG827_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0484432.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8604..9575)
                     /locus_tag="ACG827_002312"
     CDS             complement(8604..9575)
                     /locus_tag="ACG827_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0484433.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10280..11659
                     /gene="brnQ"
                     /locus_tag="ACG827_002313"
     CDS             10280..11659
                     /gene="brnQ"
                     /locus_tag="ACG827_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0484434.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11941..>12127
                     /locus_tag="ACG827_002314"
     CDS             11941..>12127
                     /locus_tag="ACG827_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0484435.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIK"
BASE COUNT         3327 a         2573 c         2205 g         4022 t
ORIGIN      
        1 atataatcat accacataca gtttttttga ctagggaaat tcctaattta aaagtaaata
       61 aaacagtaac aagattataa ttaatcgttt tcatttttat ttcctcactt ttcatgagcg
      121 ttttgaaaat tcgaacaaag aataaaataa caaaaattat taaaatagaa ttaataacag
      181 ctatttataa caaaaaaaac agagacacag cacgtttcgt gcatgctctc tgtttttcaa
      241 acaaaagact attttgtgat gatttgatcc aagcttgcta atgacaaagc catggcagct
      301 atttgactta gttgtgttag tcggttctta cgtactgctg gttcatcagc catgaccatc
      361 gtttgatcaa aataattttc gatcagtgga cgaagagcga caagctggtc gtagttttcg
      421 tggatcgttt ggtcagcaaa acttgctttc attgcttcta ctgctttgtg taattctttc
      481 tctgcgtcgt tttcgaacag ctcagggtct acttctgatt gtttttcaag ctgcgctttt
      541 ttcgctaagt tcacgacacg tgtcaaagct tccattgaag gtttgaagtc ttcatctgca
      601 agatgttgtt tgaacaattg tgctgctgca aatacttttg tcagatcttt ttgttccgca
      661 ttcaaaacag catcgatgac atcatgacga atattcttcg ttgtcaataa ttggcgcaga
      721 cggcctttga taaagtcgat cacttgttgt tgcccttctg ataaaccaat gccgaaacga
      781 tccgtatctt tactgataac atcgctaatc gtttcttgca gaacagacaa aggaaaggcc
      841 cattttttcg attcgatgat ccgaacgaca ccgtatgctt ggcgacgtaa ggcataagga
      901 tcgtttgacc cgcttggaat catcccaact gcaaagaaag aaaagatgct atctaattta
      961 tctgcaatcg ctaaaacaga acctactgta gactgaggta gctctccttc actggatata
     1021 ggcatgtaat gctcacgaat cgctgtcgct acagcaggtt tctcgccttt cagaagagca
     1081 tatttttcgc ccatgatccc ttgtagttca ggaaattccc ctaccatgtt ggttactaga
     1141 tcaaatttat agatttcagc agctcgttgc aaatccatca attcttcctc agatagtcct
     1201 acttgttcgc caatgatacg ggcaatgact tgtacccgtt gcattttctc atagatacta
     1261 ccgatttttt catggaaagt aacagttttt agtttttcaa cgtattggtc gattgaagct
     1321 ttcttgtctt cattataaaa gaattccgca tcttctaaac gagccgtcaa tactttttga
     1381 ttgcctcgaa ccacatcttc caattttacc cgatcaccat tacgagcaga aataaaatga
     1441 ggaagcagca taccttgatc attccgtacc tcgaaataac gttgatgttc tttcatagaa
     1501 gtgactagta cttcttcagg aacagaaaga tatttttctt cgaagtcgcc agaaaagaca
     1561 gttggatatt cgactaaatt gttgacttct tctagtaaat cttcgtctag atcaataacc
     1621 cagtgatttt tttctgctaa ttcgtttgct tgtgtaacga tcatttcttg tcttttttgc
     1681 ggagacgcaa tgacgaattg ttcattcaat ttttcttcat attcttttgc atgcgcaaat
     1741 gtcacatctt cgcctaagaa acgatgacct cgagaggctc tgcctgtttt gacatccaag
     1801 atttcgaatg ggattacttc gtcatctaac aatgccacga tccagtgaat agggcggata
     1861 tattcaaaat cgtattctgc ccaatgcatc gttacaggga aagtcaaact tttgatcacc
     1921 cctgctaagc ctgtcaatac ttcttttgct tccttaccat gagtgtattt cgttacatag
     1981 acatattcca caccttttaa ttctctaaaa gtgatagcgt ctgtcgtcag accttgtccg
     2041 cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc aatttttttg
     2101 gctggtcctt tgacttcttc ttcactgtcc gcttgtcgtt ctggaatatt tgttactttg
     2161 attgctaaac ggcgcggtgt ggaaaatgtt tcgatcgtat cataagtcaa ttggttttca
     2221 tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac aacatgggca
     2281 ggcatttctt ccagcccgat ttcaagtaat aagtcttttg ccatcttatt cagcctcctt
     2341 cacggtttct ggatttgctg tgtttttatt caacaaaggg aatcctaatt tttcgcgttc
     2401 tgccacaaag attttggcta cagcacgagc catgttgcgg atgcgagaaa gataaccggc
     2461 acgctctgtt acggatactg caccgcgggc gtctaataag ttgaatgtat ggctacattt
     2521 caagatataa tcatacgccg gatgaacaag attttcatcg atacagcgtt tagcttcttt
     2581 ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa aagaatattt
     2641 ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat cagcccattc
     2701 taaatcatag acactttcaa cttcttggat ataagaagca agacgttcta atccataagt
     2761 gatctcagaa gttactggtt tacaaggcag tccaccaact tgctggaagt acgtgaattg
     2821 tgtgatttcc attccgtcaa gccatacttc ccaacccaca ccggcacagc ccatggatgg
     2881 gttctcccag ttgtcttcaa caaaacggat gtcgtgttct aatggatcga ttcctaaaag
     2941 gcgcaagctt tctaaataaa gctcttgaat attctcaggt gaaggtttca tcaccacttg
     3001 gaattgatga tgctgataca aacggtttgg gttttcacca taacgtccat cagctggtcg
     3061 gcgagatggc tctacgtaag cagcattcca tggttctggt ccgattgccc gtaaaaaggt
     3121 atatgggctc atcgtacctg ctcctttttc tgtgtcataa gcttgcatca gcatgcagcc
     3181 gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca attttttact
     3241 catctgtttt gcccctttca attttaaagc gttgctgtca aataagaata caggaaagaa
     3301 aacgacaaaa aatccctatg tctgattaca gacataggga cgaataattc gcggttccac
     3361 cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg cctcttcaaa
     3421 aatcgtttgg gtccggctcc cactctcccg aactcgctaa accacgtgtt ttttactgat
     3481 cttggtcaat gtacgtattc attttgcagt tttcttgttt ctataataga tcctatcact
     3541 gaacgatcta tttaatctat ggttaataat agacggaact ccctgtgttt gtcaaatggt
     3601 tttgattttt tgttgtatgt tttatttact attcttcttt ttcaggtgtc tctttttctt
     3661 tttcagacat gtctttctct gacgtcttta atacgtgtgc ccaagacttc atttgattga
     3721 tgaacttttt gcttttcaaa tggataccga catattcatc atacagctgg tcgatgagtt
     3781 cacggattgc ttgtttggtt tcttctttca gatggattcc actaatttta tcataagaaa
     3841 tagctgaaaa catccgaacg aaataaacag ctcgaggatc tgcatgataa cggtgatcgt
     3901 ccatcgacca atgtctttcg catatcacac caccatattt ggaagaatag tcaaattttc
     3961 cttttgtttc cccgcatacg acgcagtgtg tccagattgg tgcaattccg aagcgttgca
     4021 gtatttggac ttcaaaaata ttcgtgatga tttctgcatc gtctccttgg tccatccgtt
     4081 gcagtgcttg ttgcgtaaac tggaacaaat gcgggtcgta ctgatgatct tcgattgccg
     4141 catctactag attcaataaa tatgtgccat agccgctcaa aaagatgtct tcttggatgt
     4201 gtcgaaacgc atggacatct ttgacactgc ttaaaaatga caaaccttca cttcgaaaat
     4261 tcccgatata aactgcttca gtaaaaggga gcaaagccgg agctaagggc tgattttgcc
     4321 gatggatctc tttgatataa aacatcattt tgccagaaga ttctgtaaag attttgacta
     4381 atttatcttt ttctttgtaa ttacggctag ataaaatcaa gccttttgat tctgcaagct
     4441 ggctcatatt ctccctccta aaaaataaat ctggaaaagc agattctgcc gatccagatc
     4501 atttatgttg cgatcttccg cagcttttct caacacgcgg atttttattc agtttatttc
     4561 tatacctaat ttacaagaag ctcagaatat cagtattcgt cttttcggta gccgaagtct
     4621 tgcagataga cttttttgtc tcgccagtct ttttgaactt tgacccaaag ttctagaaag
     4681 actttgtctc ctaaaagatt ttcgatatct tttcttgctt tcgttcctac ttgcttcagc
     4741 atttttccgc ctttgccgat aatgatccct ttttggctat ctctttcgac aataatcgta
     4801 gcctggatat ggattttatc attctcgttt cgcttcatcg aatcaacaac gactgcgaca
     4861 gaatgaggca cttcgtctcg tgttaaaaac aagactttct cacggatcaa ttcagaaacg
     4921 atgaagtatt caggatgatc agtcacttga tcatctggaa aatattgtgg gccctctggc
     4981 atttcactga ctaatacatc catcaaacgt tcgacgttgt ttccttctgt agcagagatt
     5041 ggaatcactt gtgcaaaagg catctgcgtt gaataatctt cgatgattcc caacaactcg
     5101 tctggatgga ttttgtcgat tttattgata atcagataaa caggagaatg cacgtttttt
     5161 agacgttcaa taataaaatc gtctccccga ccgcgttttt gatcagcact aatcataaat
     5221 aagaccgcgt ccacttcttt caacgcactg taagctgctt ctaccataaa atctcctaaa
     5281 cgatgctttg gtttatggat tcctggtgta tcaataaaaa tcaactgagc ttctggtgta
     5341 gtgtatactc cttggatttt atttctagtt gtctgtgcct tgtcactcat gatggcaatt
     5401 ttttgaccca cgattcgatt caataatgtg gatttcccta catttgggcg gccgacgatt
     5461 gcgacaaacc ctgatttatg ttctggcata tatgttctcc tttttaatta aacaacaaat
     5521 gatatatttt agggacaaat aaaatcaacc caacgatgac ggtaaaacaa gcggtcacta
     5581 aaactgctcc agctgccata tctttgattt ttttcccaat cggatgaaaa tgaaaatccg
     5641 taaacatgtc cacgacattt tcaaaaacag tattgatgat ctctactatc catacgagaa
     5701 aaacagctaa aagcagccat aaccattcta tccgatcaag ttggaaaatc acgcccataa
     5761 acaaagcgag gattccgaac gcaacatgtt tcctcatgtt gcgttcttcc tcaaaaacag
     5821 ttttgactcc ttggacggca aattccacgg acatcaggaa atgtttgttt ttctctacgt
     5881 ttttatcttt taagtccata ggcatctaag atctctttct gtaaaccaaa catttctttc
     5941 tcatcctctg gtgtcatatg gtcatacccg ttgatatgca agaatccatg aaccgctaaa
     6001 aagccaagtt cccgatcata cgaatggccg taagcttctg cttgctctgc agcacgttca
     6061 gtagagatca tgatgtctcc cagtacacga ggcaatggat tctctccatc ttcaaaaatc
     6121 actgggattt cgtcttcgcc ctcttcttcc aaggcaaaac tgatgacgtc tgtcggtgca
     6181 tcttttccac ggtaatcacg attgattact tggattgctg cattgtccat gaaagtaaca
     6241 gacatttctg tttcttctgg caactctaaa tatgatgctg caaattgcag caagtcgtcg
     6301 atttctttca ttttctcttc tgagacttgg tttgtttcat ccatgaaggt aatatccatt
     6361 atacatcgct tctttctatt cttcttgcta ttcttcttgt tcttctttta tcttctcaac
     6421 ttccgatttc attttaggat atttgatccg agaatggaag gtactgctta aaccgctgat
     6481 caatgatttt tctactttac ggatttcttt taacgtcaat ccactgtcgt ccaattgtcc
     6541 atcggaaata cgctcttcaa taagattatg gacaaaactt tcgatttttt cgctcgttgg
     6601 atggtccatt gcccgtactg ccgcttcaca actgtcagct atattgacga ttcctgcttc
     6661 acggctttga ggttttggtc caggataacg gaattgctct tccgtaactt ccgggttgcg
     6721 ttcctttgcc ttaacgtaga aataacgcat cagcgtggtt ccatgatgct gtctacaaat
     6781 atcgatcacc atttgcggca tattataatc ttccaatatt ttggcaccat ctgtcacatg
     6841 tccaaagata atatgtttac tgtcttctgg caataagaaa ttgtgcggat tttccgctcc
     6901 tgaaggcaag ttctcaacga aaaagctggc gtgtttgatc ttgccgatat catgataata
     6961 gcaagctact cgagttagta aagaacgtcc accgatttct gctacagcat tagcacttag
     7021 attggctacc atcatgctat ggtgatacgt acctggtgct tcttccagca actgtttcaa
     7081 taaaggatga ttgggattgc tgagttcatt taatacgatc acactatcat cattgaccat
     7141 caattcaata taggggtgca gacctatcgt cagcaagaat gaaaacagtg cacctgtgaa
     7201 tccgcagact agcgttaacc aagtcgttgc atccccaaag ctcattcctt ggtagataat
     7261 caaaatgaca tccatcatca caggaaaaat aatgacccac atagcagcgg ataacccttg
     7321 ttctgagatc cgtttgcgtc tgacgatcgt tgccatgaga ccagaaaata cataggacat
     7381 caaaataact gtcagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac
     7441 tgcttgaaaa ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag
     7501 tggagcaaaa gccgcggggt aaaacagcgg aatataagca gcttgttctg tttggaagag
     7561 ttggaaaaat ttcattagta cgacgcttac tgacattgcc gtcacgtaga aaagtacgta
     7621 ttttgttctt tctcctgcat tttggaattg aagtgaatta taaatcagta caccgatttg
     7681 aagcaaaaca gctaaaacca tggcaatgag tgggaaaata gaagtcgttt gattcgtcaa
     7741 tcctagtaat tccaattttt tcatcgctga agcatcaatt tgatttcctt cgcgaacaat
     7801 aatttctcct tggaagatca tcactggttg tacagcttct cgtgcagatt gttttaattc
     7861 ttctgtcttt ttctcattca atacatcatt aactacgatg ccttgatcca ctaagtagcg
     7921 aatcatttgt tgctgctctg ttgtgacatt caaatattga atctgatctt ctgcttcttg
     7981 tttaaaagct tccaattcgc tttcacgaac ttgtttactc atctgttcgt caatgagctt
     8041 caaactttca tccctaactg tgtgaatttc cgtttcagtc atttcaaata tcgtcgaata
     8101 aaaattgttc ggcagttttt ggtaaaacgc aacgttttct gcattcacgt tttcaaaatt
     8161 tttcttcagt gtagcaatcc gctcttctgt agtaggtttc gggatatttt catcttcctt
     8221 cgccttatct accttgccat tataagattt gttgatggta tcattcgtct tatcgatcaa
     8281 ttcgaataat tgcttgatcc gattgttctg gtcatctgcc agatcttttt gataagtata
     8341 ttctggagtc actgcttctg ctgccagttt tcttctttgt tcggtttctt ccgtgttctc
     8401 aatcgtttta ttcgcacgga tactctcttc tgctacttgg ccttctctga aatcaatggc
     8461 cttttgtttg acactactaa atgtaacgcc gcagacaatg ataaaaaaca agaacagaag
     8521 gaaaggcaat gctgcctttc cgagtttttc ttgtagtttc tgcagtgttt tattcaacat
     8581 gtgcttatcc tcctaagcaa agcttattct tgatgaagat ccgccttttc ataagctttg
     8641 atgatttcgg caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg
     8701 atttttttga tttgctttaa tgttcgttct gcatgaacaa gaccacttgt cgtcccacga
     8761 ggcaagtcta tctgacttgt atccccattg acaatcattt ttgattgata accgagacgg
     8821 gtcaagaaca ttttcatctg agcgactgtc gtgttttgtg cttcgtccaa gataacgaat
     8881 gcatcatcca atgtccgccc ccgcatgtaa gctaaaggtg cgatttcgat cacgcctcgt
     8941 tccatcaatc ggttcgtatg atccaatcca aaaatttggt ataaagcatc ataaacaggc
     9001 cgcagataag gatccacttt ttctttcaga tccccgggta gaaagcctag gttttcccct
     9061 gcctccacag cgggccgcgt caaaatgatt ttttgcactt cgcctttttt tagggcagca
     9121 attgctaata ctacggcaag aaacgttttc cctgttccgg ctggccctac accaaatacg
     9181 atgtcatgtt tggctaccga ttcaacgtat ttcttttgac cgctgttttt tacccgtata
     9241 ggttttccat tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat
     9301 gtaccttttt gccccatttt caatgccgtc acaacatctg gcgtacttac gtggattcct
     9361 cgtttgatca attcctgtaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgc
     9421 tcaccaatga tctgaatgat ttctccacgt gtatggatgg ttgtttccgt tgtttcctcg
     9481 atcagtttta tatgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagctgtc
     9541 aatctaatct ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat
     9601 tcattctttc ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttgt
     9661 aaaagagtcg cttaaccata cgtaccaaga aacgttaaaa aatagccgtt tccattcgct
     9721 aaaggtctcg ttttgataaa aagaagctga gaatgtaagt tatatgaaag tggagaacaa
     9781 aatatttcgt ttcattcaac aagtattctc taggtatttt tcctgtttca aaaaaagaaa
     9841 acaaaatagt taaaatattc ttgcatttca aaaaaaacag gtgtattatt acgtcaacaa
     9901 ataaacatac agcaaagcgt cgaggaaaac agtaggctat tcaacgattt attcccagtg
     9961 agtcatgtaa gtgagaaatg acaataaatc gaatagacga acatcatttc cgagtcaatg
    10021 tcgaaaacgt agatcattgt ggctgcctcc attatcaggc tagcggataa ctttatcaaa
    10081 aagcgtccgt gaatgaagaa gcttttttaa gcgaaaaaag gtggtaccgt tctagaatct
    10141 agagcccttt tcccaagtgg aaaagggctt ttttttattt taaggtggtg gttttcatgg
    10201 aatctgatat tagtggcatt actaattgct gcaacgttta tatcgtcaat caaaatgata
    10261 attattggag gaaaaattta tggaagaaac aaaaacttat tcatggaaac aactattgat
    10321 ggtcagttca cttatcttcg ggatgttctt cgggtcaggt aatctaatct ttcctgtcca
    10381 cttaggtcaa atggctggcg caaactgggt cagtgctggc gtaggatttg ctatctcagg
    10441 tgctcttttc ccattactag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta
    10501 tgacttagct aaacctgtcg gaaaatggta tgctgccttg ttcttaatcc ttgttcattt
    10561 gacgatcgga ccatttttcg gtacaccacg gacagctgct actgcttttg aaatggctgc
    10621 taaaccattt ttgcctgaaa aatataattc attaggtatg tttttattct ctgctctttt
    10681 ctttttactt gcttatttcc ttactgtcca tccaacacgg ttggtcaaat acgtcggaaa
    10741 gtatctaaac tccttgtttc ttgcattatt agcaatcatt tttgtcgttt ctttattcca
    10801 tccaatggga aatctaaatc aatctgctgc agatacttat caaacaaacg ctttaacaaa
    10861 tggtgtccta gaaggttata acacggttga tgcagtagct ttgcttgcct taagtgtcac
    10921 ttttgtccat gcagtccgtg accttggttt caaagataaa aaagtcacag aattaacggc
    10981 aaaagctggt agcctgagcg ttgcgctaga agtcatgatc tattttggac ttgttcttct
    11041 cggtgcgatg agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca
    11101 gatcactaat ttttatttag gtaacttcgg cgctagcttc ttaggagtga tggtcacatt
    11161 aggtgtcttc actaccgcta tgggactcgt tgtttctttt gcgcaagatt tccataaact
    11221 gttccctaaa gtcagttata tgacatggct gcggttaacg actttcgtct cttttgtcgt
    11281 tgcaaatgct ggtttagaca atatcatcca atggtcttta ccagtattga tgcttttgta
    11341 tccattgtcg cttgcgttga tcttgctttc cttaacagca aaatttttcc aaaagacacc
    11401 atttgtctat caagtaacga tgttgtttgc tgctgttcct gccgtattgg atatgctggc
    11461 aaattcgcct gctttagtga gtcaacaaag agttgtagca agcatgctcg aattttatca
    11521 tcatcacgtt ccttttgctg cattaggact tggctggatg gtcccaacgt tacttggtta
    11581 cgctggcagc ctactgtttt attacgccta tcgcctatca gggtataaac aagaagcaaa
    11641 tgaactacca gaagaataac caaatcaaac acaatttaac ataagaaata ttaaacagga
    11701 aaaaaccagc tagatgaaag aatcctattc tttcgtctag ctatttttta gttctctttt
    11761 ttacgcgcac aaaaaaactg cggacaatga ttgtccgcag tttaaatggg ctctataata
    11821 tctagtgttg gtgacgcctt ctgttgccaa tactttttct tttttctaat aaacgcaaac
    11881 agacaccttg aaatccttta gaataaagtc gacgaaaacc attcaaagga gtgtttcaag
    11941 atgtcctatt cagagtttac caaagagtta ctagatattc tagacctaaa tcttatcttt
    12001 catgaagatg cctttcgcaa agagcgaatc aatgacgaaa catgttttgt ttttgatggc
    12061 acattgacct atcaaccaga ggaatgcttc cattgtcact atcaaaataa gcaaacgatc
    12121 attaaat
//
LOCUS       DAZKOU010000088        11695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000088 DAZKOU010000000
VERSION     DAZKOU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.089"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..2055
                     /locus_tag="ACG827_002315"
     CDS             37..2055
                     /locus_tag="ACG827_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0484436.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2125..2331
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2482..4896
                     /gene="leuS"
                     /locus_tag="ACG827_002316"
     CDS             2482..4896
                     /gene="leuS"
                     /locus_tag="ACG827_002316"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0484437.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5244..6797
                     /locus_tag="ACG827_002317"
     CDS             5244..6797
                     /locus_tag="ACG827_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0484438.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7178..8962)
                     /locus_tag="ACG827_002318"
     CDS             complement(7178..8962)
                     /locus_tag="ACG827_002318"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484439.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8977..10698)
                     /locus_tag="ACG827_002319"
     CDS             complement(8977..10698)
                     /locus_tag="ACG827_002319"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484440.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10798..11559
                     /locus_tag="ACG827_002320"
     CDS             10798..11559
                     /locus_tag="ACG827_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0484441.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3815 a         2139 c         2377 g         3364 t
ORIGIN      
        1 attaataata gataaattga agttggagga tagaagatgg aaaacattgc aagaaaagaa
       61 cggagacgtt tagaacagac caaacgtttt cgaaaagcaa aacgaggggc agcaatcgtg
      121 ggaactgcga tggtaggctg ttctgtcgct gccccattag tacagccggt tcctgttcaa
      181 gcggatgaaa cacctgttca gttcggcgca agaatcaata cagcggcatt tatcacagaa
      241 attgctactt atgctcaacc aatcgctcaa gccaacgatc tatatgcatc agtgatgatc
      301 gcacaagcag ttgtagaaag cggttggggc agtagtgcgt tatctcaagc accttattat
      361 aatctttttg ggataaaagg cagctatcaa ggacaaaccg tctacatgga tacgcttgaa
      421 tttttaaata atcaatgggt aaataaaaag gagcctttcc gacaatatcc ttcttttgct
      481 gaatctttca atgacaatgc ctatgtactt agaaatactt catttggcaa cggttattac
      541 tatgccggta cttggaagag caacacgaaa tcctatacag atgcgacagc ttgtttgaca
      601 gggcgttatg cgactgatcc tggttacgca ggcaaattaa ataatatcat cgcaacttac
      661 ggattgaccc aatatgatac accagctagc ggaaacgcag gcggtggagc gactacaggt
      721 ggcaataata ctggaagtgc aggtagtact ggcggttcaa gcaattctgg taatacaaac
      781 tcaggttcag caacttcgac cacttatact gtccaatctg gtgattctgt ttggggaatc
      841 tcaaataaat tcggtatcac aatggatcaa ttgatccaat ggaacaacat ccaaaacaat
      901 ttcatttatc caggacaaaa actgacaatc aaaggtagcc aggaaaatgg ctcttcgacg
      961 aataattccg gtaacaatac caattcttca ggaaatgctg gtacatcaaa cggaggacaa
     1021 acaactggtg cgaaatacac tgttcaatct ggtgattctg tttggaagat ctcaaatgat
     1081 cacggaatta cgatggctca attgattgaa tggaacaata tcaagaacaa tttcgtttat
     1141 ccaggacaac agctaatcgt atcaaatggc ggttcttcta atgctggagc aaatactggc
     1201 tcaaatactg ggaatacaag tggctctagt tcgtccagct caagttcttc taatacagct
     1261 tcaggaacga aatatacagt taaagccgga gactctgttt ggagcgtctc aaataaatac
     1321 ggaattacga tggctcagct aatccaatgg aacaatatcc aaaataactt tatctaccca
     1381 ggacaacaac tgactgtttc aaacagtgga tcaacaaaca gcacgtcagg taatacaggt
     1441 tcgtcaacca atacaaataa cagcagttca gttaatactg gcagcagttc gtctcaaggc
     1501 acttataccg tgaaagcagg tgaatctgtt tggagcgtgg caaataaaaa cggcatcacg
     1561 atggatcagt taatcgaatg gaataatatc aaaaacaact tcatttatcc aggacagcaa
     1621 ttgatcgtta aaggtggaac ttcggcaaat acaaatacag gttcaactac ttctgcagcg
     1681 aaaccaaata caccgaatac gtctgctacg acaagtacat catccactgg taatacgatg
     1741 tataccgtga aagcgggcga gtcagtttgg agcgtagcga acaaacatca tatcacgatg
     1801 gatcagttaa tcgaatggaa taatatcaaa aataatttca tctatccagg acagcaagtg
     1861 attgtgaaga aaggttcatc tcaatcaaca aaccaacaat cagttactgg ttcgaaaacc
     1921 tatactgtga aatctggaga atctgtttgg agtgttgctg acagtcatgg aattacaatg
     1981 gctcaactaa tcgaatggaa caatattaag aacaacttta tttatccagg tcaaacgttg
     2041 attgtagcta aataaaatag ctgttcgaag aaataacgat tgaattccac aaaaatattt
     2101 gttactataa ggctacttaa atatgacaac acttgaaaag gaagtagtaa aaagactgaa
     2161 gtttcagaga gcgcatggct ggtgagaatg tgcacggatg cttttgaact cgcctttgag
     2221 ttgcccttgc tgaatacccg agtaagcaag cggcggtgac ggccgttaca acgttcgagg
     2281 ccttttcact aaggtaaaat taggtggtac cgcgtagatt acgtcctata aaaagagcaa
     2341 tctttttata ggattttttt atttcttttg tgaagtttga gaaaacaaag caaaaggaaa
     2401 taaaaataaa cagattctgc cggttttttc gttatattta taatagaaac aagtgttttc
     2461 aatagaacag gaggacagca aatgagttac aatcataaag aaattgagaa aaaatggcaa
     2521 aaatactggg caaaaacgaa tactttcaac acccatgatg atcctgaaaa gccaaaattt
     2581 tacgcactgg atatgtttcc ctatccatcc ggtcaagggc tacatgtagg gcatcctgaa
     2641 ggttatacgg caacggatat cctttcacgt gtgaaaagaa gtcaaggata caacgtgctg
     2701 catccaatgg ggtgggatgc attcggtctg cctgctgaac aatatgcgct agatacagga
     2761 aacgatccag ctgagtttac ccaaaaaaat atcgaaacat tccgacgcca aatcaattct
     2821 ttaggattta gttatgactg gaatcgcgaa gtgaatacaa ctgatccgga gtattataaa
     2881 tggacacaat ggatctttac gaagctttat gaaaaaggtc tagcttatga agcagaagta
     2941 gcggtaaact gggtacctga attaggaaca gttatttcaa atgaagaagt gatcgatggg
     3001 aaaagtgaac gtggtggcta tgatgtagtc agaaaaccaa tgcgccaatg gatgctgaaa
     3061 atcactgctt atgctgatcg cttattggat gatctagaat tagttgattg gccagaaagc
     3121 attaaagaaa tgcaaagaaa ttggatcggt cgttcagtag gagcaaatat tgaatttaaa
     3181 gtggctggta cggataaatc ctataccgta tttacgacac gaccagatac tctattcggt
     3241 gcaacttact cggttcttgc acctgaacta gatcttgtta gagaaattac gacccctgaa
     3301 caaaaagctg ctgtagaagc ttatatcgaa gagacagcta aaaaatcaga tctaaagaga
     3361 acggatcttg caaaagaaaa aacaggagta tttactggtg cttatgcaat caatccagtt
     3421 aatggcaagg aaatcccaat ctggatcgcc gattatgtat tatcttcata cggcacagga
     3481 gccatcatgg ctgttccagc acatgatgaa cgtgactttg aattcgctca aactttcgga
     3541 ctagaaattc ttccagttat cgaaggtgga gatgttgaaa aagctgctta cacagaagat
     3601 ggtacgcaca tcaactctga atttctagac ggcatgaaca aacaagaagc aatcgataaa
     3661 atgaatgaat ggctagaaga aaatggcgta gggaaaaaag aagtcagcta tcgcttacgt
     3721 gactggctat tctcccgtca acgttactgg ggtgaaccaa tcccaatcat ccattgggag
     3781 gacggaacag tcacagctgt cccagaagaa gagctaccat tacggttgcc taagacaaaa
     3841 aacatcaagc caagcggtac tggagaatct ccattagcaa atatcgagga gtgggtcaat
     3901 gtcgttgatc ctgtaacagg taaaaaaggc cgtcgcgaaa cgaatacaat gccacaatgg
     3961 gcaggaagct catggtatta cctgcgttat atcgaccctc ataacaaaaa agaactagca
     4021 gattatgaaa agttgaagcg ttggctacca gttgacatct acattggtgg agcagaacat
     4081 gcggtacttc atttactata tgcacgattc tggcataaat tcttatacga tatcggtgtt
     4141 gttcctacaa aagaaccatt ccaaaaacta tacaatcaag gaatgatctt aggcgaaaac
     4201 aacgaaaaaa tgtcaaaatc tcgtggtaat gtagtcaatc ctgatgatgt ggtcgaaact
     4261 tacggtgcag atacactgcg catgtacgaa atgttcatgg ggccattaga tgcttcgatt
     4321 gcttggagcg aaaatggctt agaaggaagc cgtaaattct tggatcgtgt atggcgtttg
     4381 atcgttgatg agaacaataa aatgcgtgac cgtatcacta cattgaatga tggcaagctt
     4441 gataaagtgt atcatcaaac agtgaaaaaa gtgacagaag actatgaaaa tcttcatttc
     4501 aatacagcaa tctcccaatt gatggtcttt atcaatgaag cctataaagt ggacgctctg
     4561 ccttatgaat atatcgaagg atttgttcaa ctactcgcac cgattgctcc tcacatgggt
     4621 gaagaactat gggcaatcct tggaaatgat ggtggtattt cttacgcacc atggccaaca
     4681 tacgatgaag cagcacttgt tgaagacgaa gtagaagttg ttttccaagt aaacggtaaa
     4741 gttcgtgcga aaagcaatgt tccccgagac ttaggaaaag atgagctaga aaaagcagca
     4801 ttagccaatg aaatagtgca agaatatatt gaaggcaaaa cagtcagaaa agttattgcg
     4861 gtaccaaaca agttagtcaa tatcgttgct aattaattgg ctctttgtca ataaggactg
     4921 atgagttgtg caaaatcaaa tctgggccag aatgaacttc attctgtctc agattttttg
     4981 ttatttcaaa ttgattaatt gattgatcaa aaaggttttg attttcatgt tctcaaacac
     5041 gttcaatgtc tttttcttct gaatggtctt gtaagagatc tctgtaatca tcaaattttt
     5101 gtctattaat cttagtattt tttgatgtat aaactacctg aaattgagag tacataccaa
     5161 attcataaat tttttattag tgggactagt gattgtatgg attttgacgc ataatgaatg
     5221 aagtatgaga aaaggagaga caaatggatc aagaactatt tattatcgaa gtattatcag
     5281 ctaaacaaaa aattatcgag aaagcgaaaa aaagagcgaa aagaataatg ttcaagcttt
     5341 taagcttagt atttttctct ggtttgcttt ttttcggagc tgtatcgttc ctatatcgcc
     5401 atatgagtgc agtagtccgt tcagctgaag ggatgcaaaa attaaataat ttgtatcact
     5461 tttcgtgggg agttggcgga ataacaattg ttgtgcttat tgtttttctt ctacttattt
     5521 ggctccgtta ccatcaattg tcgcgtcatt tcgatcaatg gcttagtcaa agagcacaac
     5581 agtttttaca taataaacca acttctcaag atgatgacta tctttatttt gagaagactt
     5641 cgacgagtca agcttttcgg atacaaaaaa gtgattgcca gttattgctg agatcacttg
     5701 atgatttacc tatctactta ggcgaaagca aatcaaaatt cggaatgaac acattccata
     5761 tttttttact agaaaacgaa cccagatggg aaaaaagcca gtcttcgcct ggtggatacc
     5821 tgacaactaa gatgagtaca ttagtcactg gttttttcct cattctttta gctggaacaa
     5881 tctattatgg gagatacact cagccaaaaa tgtcttctca tataaatgct gcttctcaca
     5941 tgacaatgaa ctcaactaac gatacgcata tacaaaagac aaatgaaggg ttgttagctg
     6001 atcctaagca aaaggtaaat ttgacaacac aaaagataaa tgatctcaaa cttgatacag
     6061 agacgcactc tctctatatg acaacagata gtggtgctaa ttgggtattt gtgccgattg
     6121 attcgcaatg gttgcgtttt ggcgaataca ccctgacaac ggggctgatt cctgaaggtt
     6181 attggatgga caacacatat gatatttccg ccgatttctc atggtttatc tattcttctg
     6241 gggatgatgt acgtctgctg acttcaagag ataatggtaa aacatggcaa acgtctatta
     6301 tcacccaatc agttcagccg atacgttatc gaaaagcggt attttatggc aacgatggtc
     6361 tatttatctg tacatcagcc agtgggatgt ctgcagaaac tttgtacatt tacactacta
     6421 tggatggcgg aatcacttgg ataaaaagtg gaagtacaat gatcaatcag ccagtacaaa
     6481 atgtcagctt cgttacaact tctttaggtt ttgtttcgac acgtgaaagt ttgttttata
     6541 caaacaacgg aggacgctca ttcaaagaat cagttctgtc gattccggaa aactatcaga
     6601 caggggggat agatcttttt caatcaccaa atgaagttac tcaaataagc gcgaatacgt
     6661 tagagacaaa attttatttg ttgaaaaaag gaccaatcga tgcagggaaa atgtttgcct
     6721 gcctcttccg ttcaactgat aacggggaaa catggcaatt cgttcagcaa ctatcgcaag
     6781 tagaacaagg agattaaaga agagagattg tgagcctgac gttcagcacc gagtcacaag
     6841 aaacaaattc aaaaaacagc ttctcatatt tgttgaatag tgtaactcgt gattcggagt
     6901 tgacaagagc aacaagaagc gattttgaag tatagcttct cgtattttac aagatcgtgt
     6961 cttattgccg ataggttgtc tcttgaacac cgtttattcg taaaaaggga ttgtgacaag
     7021 tcttttgtca ctacttaccg aacgaaactg aaggagctat ccaaaaagta tcattagtat
     7081 tattctttgt gtttctagtg gcaccactag tacatttaaa aaagctatcg atcatggact
     7141 aatcatacat gatcgatagc tttttagata atgtttgtta cgctatctga aattgactat
     7201 tataaagact ggcataaagc gttggtgatt ggagtagttc agaatgtgtt cctttttcga
     7261 cgatatcgcc attcttcatt acaaggatca tgtctgcatt ttcaatcgtt gacaaacggt
     7321 gtgcaatcac aaaacttgtc ctatcttttg tcacagcttc cattgcttgc tgaatcaatg
     7381 cttctgttcg tgtatctacg ctagaagttg cttcatctaa aataacgaca ggaggattcg
     7441 ctagaatgac tcgagcaatt gtcaataatt gttgctgtcc ttgagataac gtgccgtttt
     7501 cgctagaaat gatcgtatca tatccgtctg gcagtgttcg aataaagtga tcacattgtg
     7561 cgatttcagc cgcttcgata atttgttctc ttgtggcatc cattttcccg taggcgatat
     7621 tatcagctac cgttccttca aacagccagg tattttgtaa aaccatccca aaagaacgac
     7681 gtagttctcc acgtgaaagc ttggtaatat caagaccgtc gaattcgatc tttcctttat
     7741 tcagttcata aaaacgcatc agcagattga ctaaagtcgt ttttcctgca ccagttggac
     7801 caacgatcgc taccgtttgt tttggcttta ctgaaaaatc gacttgattc atcaatattt
     7861 tatctgatgt gtaaccaaac tgtacatttt tgaaagctat ttttcctttc ggttgttcta
     7921 aaagtcttgc atctggaagg tctggaattt catctgtttc atctaatatc tcaaatatcc
     7981 tatcgattgc tgccattgcg gattggatcg agttgatcac ataagaagcg gtagaaattg
     8041 gttcagaaat ctgattgatg tactgaagat aagcttgtaa gaagccgatc gtcacaccac
     8101 ctgatagaac aagcatagca ccgaataccg cacttatgat gaaggcgagt tgattaatga
     8161 aacgaatggc cggatagata gcacagttca taaattgagc acgtttaaaa gcgttgtatt
     8221 gctttttatt tacttcgtgt atagcagctt ctgcatgagc ttgctgattg aatgttttga
     8281 ccactagatt accagctaag atttcttcca ttcgattatt taaaacaccc agttcatact
     8341 gattctgttc agaaatctgt ttgtttttgt tcgcaatctt agttgtaaca attgaactgc
     8401 caacgatcag caataagacg agcaaagtaa gcttgctttc gatataaaac agcatcagac
     8461 cagcagcagc tattgttacg acagaagtaa aaaattgatt gatacctgtc aataacactt
     8521 gagaaagttg attgagaccg gtagttgtcc gactaatgat gtcacctact tgatgattgt
     8581 caaaaaacga catcggtaat gttttgaatt tttttgtgat ttcttttcgc accctcaatg
     8641 tgatcttttc gcttaacgct gccattgtcc gttcctgaat aaatgaagtg atgctgctta
     8701 agactgaaca gccgatcagc aataagacag ggaacaacaa tgcttgtttg atatcactga
     8761 aagaaagctg tccaatacct gtttttctta tttctgataa gagatcatcg atcccgattc
     8821 ccatgatcaa gggcatggct acgactaata ggttcccgaa taaactgcat agaaacaatc
     8881 cgtaaaaata tttatgttca ggtttgatca ttttccaaaa acgtatcaaa gcacgcgtgt
     8941 tcaacgtttt tttcttttcc atcgtatccc ttctttctat attccttgtg attttgcaaa
     9001 ctcctgataa gtttggcaat tagacagtag ttcttcatga gtaccttgtc caacgatttt
     9061 tccttcatct aaaacaatga tgttatctgc ttgttggatt gtactgaggc gttgagcaac
     9121 aatcacgaga gttttctccg acatgtgttt atgaagggct gctcttaatg ctgcatctgt
     9181 tttatagtct aaagcggaga aactatcatc gaatacatag atctctgctg gtttgatcaa
     9241 cgctcgagca atacatatcc gctgtttctg gccccctgaa taattagaac cgccttgagc
     9301 aacaaagctg tccaatccgt caggaagggt gtccactagt tcttctaatt gagcgatctt
     9361 gatcgcttta gacaactctt ctgttgaggc atattcattt cccatctgca agttgcttaa
     9421 aattgtcccg ctgaataaga aagccttctg agggacataa ctgatgtggg agcggatctg
     9481 ttcttgagtc atttgagtaa gaggaatacc accaagggtt acttttccta tcgtagggtc
     9541 actaagtctc aagagaagtt tcgccaaact actttttcca gatccggtcc cgccaacgat
     9601 cgctgttgtt tttcctttag gtattgtgaa atcaatatct tctagaactg gatcagagat
     9661 ttcagtatat ttaaatgtga catgttcaaa agcagctgca tttgtctgat caaaggttgg
     9721 ttcagctact gttttgatcg tctcttcctc aatttcgatc tctgcttgca gtatttcttc
     9781 tatccgtcgt aatgaggcaa acgatttagg gataatgacc aataccatag ctgcgatcat
     9841 caagtaagca agtgtcagca tagagtattc gactacagca gtcacgacac cgatttgtaa
     9901 tgctccttct gctacaagat ttccgccaaa ccacaaaata gaagcataaa cgattcccat
     9961 gagcaaaaaa gcaattggag tgacccaagc aaatattcgg ttcactttga tcatttgatt
    10021 agcatagtca gcaaagctct tattcgttcg cttttcttca tatgattgat tatcaaaggc
    10081 acggatcata ttaatcccag taaagaattc tctcaatgtg atggtgatcc gatccatttt
    10141 tggctggata ctcaaagata aaggtgcccc tcgtttcatc agtcgataaa caatccatac
    10201 ataaaaaaca acagagatca atgtaaccca tgttagtaat agagaataac gaaacatcat
    10261 gagaatggtg aaaacgcaaa taaccggtgc tggtaagatc atttggcaaa ataaaacaat
    10321 catttgctgg acattatcga catcattcgt cattcttgtc aaaagagatc cagaactaaa
    10381 acgactgata tccttcattg acagctgttg gaatttacgg aaatatttct cccgcgtttg
    10441 gaatccaaac ctagcggaag ccaatgctga aaagtaacta gaggcaactg cagcgattgt
    10501 tcctagaaac gctacaagca gcatttcaac tccaattttt tttataactg aggcttcgcc
    10561 acttgctata cctgtgtcga tcagctttgc tactaatttt ggtacaccta atgtaccggc
    10621 gatttctaaa cagatcatca caaaggtaag gagagcgagt cctttgtttt tctgtaagaa
    10681 tgtttttaag atagacatat tattcctcct tttttcctga tacttttcga ttctatacct
    10741 tcaagtaact tgaaggtcaa tagaaaaaag aataaactga aaaagaggtg agaaaaaatg
    10801 aaaaaaatgt atacgattgg ccaagtatcg gaactatttg atttgccaaa atcaacgatc
    10861 cgctattggg atgaacaagg attaatccgt tcaagtagac aagaagagaa tgattatcga
    10921 ttgtttgata tcgatgatat ttttatgctt tatgatatcg ttttttatag aaaattagat
    10981 ataccgatca aacagatgaa gaatttatat ggaaaaacat tgacggagtt atatgagaca
    11041 cttgatgaaa cagaaagaag aatccataaa gaactggtgg tgatgaaaca aaaacaaaaa
    11101 gagataaggg agcaaaaaaa acagttgaaa ttgatgatcg atacaaacga ggaagaattt
    11161 cctgtagaag agatcccttt tgactgtatg atctcaacgg agtttgaaga tattgtggaa
    11221 atcaaaaaat ttctacctaa ctattcaagc tttgggatga tgagtatgcc agcttccagt
    11281 tcacagacga tgtatggatt ttttatcgac cctagtgaag tacatttatt tactcaagac
    11341 gtgatttggg agaaaaaaga tacggctgtt tatcgtcgat tcttattgaa atccgagatg
    11401 aaccatgcgg aacgtaataa tattttagaa atcagagaaa gaatgtatga aaaaggatgg
    11461 aaaacaggag aagtgatcgg ccagtatcta ttgacgaata cggatgaaaa caatatcaga
    11521 actgaatatt atcatgcatg gatagaaatg aaaaaatagg aaatatgtgt ttaagataac
    11581 tcgaaaatga tggttgtttt agttataaag aaaaattttc tggttcgatt acgggtttaa
    11641 tgtgggggat tattagtcgt ggatagatag aattttagtt ttttagagga aatat
//
LOCUS       DAZKOU010000089        11504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000089 DAZKOU010000000
VERSION     DAZKOU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.090"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..260
                     /locus_tag="ACG827_002321"
     CDS             93..260
                     /locus_tag="ACG827_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0484442.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(320..673)
                     /locus_tag="ACG827_002322"
     CDS             complement(320..673)
                     /locus_tag="ACG827_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484443.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1177..1596
                     /locus_tag="ACG827_002323"
     CDS             1177..1596
                     /locus_tag="ACG827_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0484444.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            1622..1828
                     /locus_tag="ACG827_002324"
     CDS             1622..1828
                     /locus_tag="ACG827_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484445.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(2056..2229)
                     /locus_tag="ACG827_002325"
     CDS             complement(2056..2229)
                     /locus_tag="ACG827_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484446.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            2241..2870
                     /locus_tag="ACG827_002326"
     CDS             2241..2870
                     /locus_tag="ACG827_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484447.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(2952..4118)
                     /gene="dnaJ"
                     /locus_tag="ACG827_002327"
     CDS             complement(2952..4118)
                     /gene="dnaJ"
                     /locus_tag="ACG827_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0484448.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(4269..6098)
                     /gene="dnaK"
                     /locus_tag="ACG827_002328"
     CDS             complement(4269..6098)
                     /gene="dnaK"
                     /locus_tag="ACG827_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0484449.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(6149..6712)
                     /gene="grpE"
                     /locus_tag="ACG827_002329"
     CDS             complement(6149..6712)
                     /gene="grpE"
                     /locus_tag="ACG827_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0484450.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(6806..7849)
                     /gene="hrcA"
                     /locus_tag="ACG827_002330"
     CDS             complement(6806..7849)
                     /gene="hrcA"
                     /locus_tag="ACG827_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0484451.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(8083..9248)
                     /gene="hemW"
                     /locus_tag="ACG827_002331"
                     /pseudo
     CDS             complement(8083..9248)
                     /gene="hemW"
                     /locus_tag="ACG827_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(9264..9491)
                     /locus_tag="ACG827_002332"
     CDS             complement(9264..9491)
                     /locus_tag="ACG827_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0484452.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            9975..10582
                     /locus_tag="ACG827_002333"
                     /pseudo
     CDS             9975..10582
                     /locus_tag="ACG827_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            10755..11468
                     /locus_tag="ACG827_002334"
     CDS             10755..11468
                     /locus_tag="ACG827_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484453.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         3340 a         2319 c         2011 g         3834 t
ORIGIN      
        1 ggtttcttgt taaataagat gaaatatttt ttaataaaga tttgaattaa agtgtaaagg
       61 aggagatagt tattataaac tacaagtgga tattgtgtcc tgtatgtgga aataaaacac
      121 gattaaagat aagggaagat actgaattaa aaaaattccc cctctattgt ccgaaatgca
      181 gacaagaaaa tttaattgaa ataaagcagt tcaaagtaac tgtgattaca gagccagacg
      241 caaagacgca gagccgataa aatgagatta atacaatctc attttatcgg ctctttccgt
      301 tatgtatgga ttcttttaat tagtcttcga tgtttcttgc ttcgttgata ccgctggcta
      361 aagattccat taaggatagt tctttgtctg taaagctatc catgtatttc tctatctgta
      421 atcgtcgggt gctttttacc aagttattag caggtaagaa aaattcatca acggaaacat
      481 gaagtaacga tacaaggtca taaagaactt gtatgctggg gtgttgccct ttattttcaa
      541 tattagttaa gtaccgtggg tcaatttcaa tcaatgctcc cacttgttca cgagttaaac
      601 ctcgtttcaa tcgagcttct ttaatggcta aaccaaaggc tctaaaatca tatttatctt
      661 cttttttacg catagtagac cacctctata cattttattg ttcctactga attaaaaaca
      721 ggtatagaaa aacgtgttat atggtttata ggtttatatt taataaaaag cactactaaa
      781 cgccaataaa aaaaaccgtt atatggtagt gctatttacg ctgttaaaat attgtatatt
      841 acttccaaat ggcggtttgt tggaggtcaa cgtcgccatg aagtacatca tatacaataa
      901 atttccttac attgggttct tgtcaaaaaa agtcgtctat ctgcaataga taagtacgtc
      961 caccaatgtg gttttataaa tcatatagat agaataacag aagcatgtaa acagagaaat
     1021 aaatctgttt atatgctttt ttggctattc agaacttttt tacaaagttt atttatcagt
     1081 aatgcaacaa atcccccttt cacattggga ctaagagtga aaggagataa acgagcaagg
     1141 ctcacttcct ttcctagaca gaaagggggt gagattatga ggacatcttc tttcgagcat
     1201 atcgtcagat tacagttcaa ttctttaatg ctgattgtca ttaagaacaa agtgaaaagt
     1261 cgaaacagac agcttgcaag acattctaaa cgtgaagtat tgttttgtga aatagccgaa
     1321 acgaaacaag ttgaacgtgg aacgattgat acctgttttt atgattgtgt atcatttaaa
     1381 gtcttgcact ttacaattta tgtatctgat gaaacacttg gtacagtttt acatagacta
     1441 tcagaaaaac agcgtagtgc gattttatta cgttattttc aaggaatgaa cgacagagaa
     1501 atttcagaac tgtaccatgt atcacgttct gctatttgca gtagaagaac tagaggacta
     1561 aggaagttaa aaatgctttt gagcgaaagg aagtaaagat tatggctatc cctcttttga
     1621 attgatttgc aaagcgtcaa gcggagatga aatggcagtt aaagagattt tgaagtttta
     1681 tgacggttat atttctaaat tatgcttgcg tcctttttac cactctgaaa gtggtaaaat
     1741 cattatgcag gttgatgaag aactaaaagg agaaattcat acagacatga tgaaagcaat
     1801 attgaaattt gaaatcggag tgaagtaatc aactgtataa aaaaatcggg agatacacag
     1861 tttcgtgtta tctcccttag tagttttacg gtcatatcaa ggctcattca atcgtggtaa
     1921 attcgtggta agatgagtgt ttttctatac ttcaaaatgc ttgaatgtat agtgtttatg
     1981 cggataattg aaaattagat ataaaattta ataaaaacca aaaatgcacg gtcgccatga
     2041 agggaccgtg tatttttatc ttcttatttt ctcacctaat cgtatcagcc gcatcgcttg
     2101 ttttcctgca caaacaagca atcgctctac ctgttccatc gcataagaaa gtaccatcgg
     2161 ttcattgatg atatcaagta catagtcgat accaccctca cttaattgag cttgattttt
     2221 cttctccata ttatgtagtt gtgggacaac tttctgacaa tcagcaattg tgtcaagaac
     2281 ggatcaatcc tttattggag ttgctcgaaa gggtcttctc ttattacgga caggctttgc
     2341 tcactgtaca tagacaacaa atcattatcc tagtcaatcg gatttccaga gcctcattgc
     2401 tatcattgct tgataaaatc cagacaaagt ttaataaaat gtatcagtta cagctaaact
     2461 ttggaattgg gtcgctttgt tatacagagc aagagacacc gcaatctttc cttcatgcca
     2521 aacaagtttg cgaatggata gctttccatc aatctgtgaa cgagatccgc ttttttgaag
     2581 atttagacct cgggatcgtt ttaccagcaa tcccgtcaga tcaacgaaca ttatatgtaa
     2641 aacgtatttt gaaaagcctg acagaagaag aagtacattt gttcaagaag actttggcat
     2701 gcttcagtaa aaataacgga agcatcaaaa attgctcaga agaattattt attcataaaa
     2761 acaccttgca atatcgattg aacaagttcc attctctcac tggttatacc cctagaaact
     2821 atgacgatta tcatattcta aagctggctt ttttgttggt acaaacatga caactacaaa
     2881 aaaaccaggc gaacatatga ttgttcgtct ggtttttaat tattaatgac ttagcgtttt
     2941 tactagaaaa actacttccg ctttttcccg ccaaaagcat ctttaaactt atcgaagaaa
     3001 ccgtcttctt gctgttcttc aactttgatg ccgccagctt cagcgaatgc acgtaatgct
     3061 tctctttgtt gttcatttag atttttcggt gtgatcagtt ttactttgac atgttggtcg
     3121 cctgttgcac caccacgtaa cttcggagcg ccttttccac gtaaacggaa gttcgtacct
     3181 gtctgtgttc cagcagggat cttcaatttc acatctccat ggacagttgg tacttggact
     3241 tcatccccca aagctgcttg tacaaaactt aaaggcaatt catagtaaat ttcagaacct
     3301 tcacgatcaa agatatcact ttcttctaca cggaagacca catacaaatc accaaatggt
     3361 ccgccgtttt ctccagcttc tccttgacca gctaaacgca tttgttgtcc ttcttctaca
     3421 ccagctggta cattcacttt aactgaatgt gctttcttct catgtcctga gccatgacag
     3481 gttggacaag gttctttgat ttctttccct gtcccatgac aaacatcaca agtttgacgg
     3541 ctcatcactc gtccaagtgg tgtttggcgt tctacattaa tcgtacctgc accatgacat
     3601 ttatgacaag tagttggttg tgtgcctggt tttgcaccgt tcccaccaca agtatgacag
     3661 atttcttccc gattatactt gatttctttt tctacaccaa aaatcgcttc ttcaaaagac
     3721 aagtcgaccg tatattgcaa atcagcacct tgacgaggcg catttggatc gactgaccga
     3781 ccgcctcctc caaagaaaga gtcgaagata tcttcaaaac caccgaagcc gccaccagag
     3841 aagccgccga atccaccaaa tccgccacca gctccaccag caccgtaatt tggatctgtg
     3901 cctgcatgcc cgtattggtc atatgcagcg cgtttttgcg ggtcacttaa aatctcatat
     3961 gcttctgata cttctttgaa tttttcttcc gcatcaggtt ctttattgat gtcaggatgg
     4021 tattttttcg aaagcttacg ataagctttc tttatttcat cttctgatgc tccttttgag
     4081 agccctaaga cttcataata atcacgtttt gttgccatag gctttcctcc tattggttct
     4141 catcattttc acgtcgcttg ttatcatatc atattttgtt tgaaacaaaa accgatactt
     4201 caaactattt ttcaaaagaa aaagccaaag tagcaacttt ggccttctct cgaaattcag
     4261 tgaactgatt atttatcgtc gccatcgact tcttcaaaat cagcatcaac gacatcatct
     4321 gctccgcctt gagctgcttc tgggttttct tgagcttgtt gttgtgccgc ttgttcgtat
     4381 agtttaacag tcaagttttg aacgatttcg ttcaatgcat cgcgtttagt tttcatttct
     4441 tcgatgttgt ttgcttcaac agcggctttc aattcatcgc gagctgtttc tgcttttttc
     4501 acttcttctt cgtccacttt gccttctaat tctttcaatg ttttgtccac agagaatagc
     4561 aatgcatcta catcattacg taggtcaact tcctctttac gagctttatc tgcttctgca
     4621 tttgcttctg catcttttac catacgttcg atttcttcgt ctgttagacc tgaagaagat
     4681 ttgatcgtga ttttttgttc tttttgtgta cctagatctt tcgcagatac atttacaatc
     4741 ccgtttttat caatatcgaa tgttacttcg atttgtggaa taccacgagg agctgctgga
     4801 atatctgtca attggaatct tcctagagtt ttgttgtcag cagccattgg gcgttcacct
     4861 tgtaatacat ggatgtctac tgctggttga ttatctgccg cagttgagaa tacttgtgat
     4921 ttacttgttg gaatcgttgt attgcggtca atcaatttag tgaacactcc acccattgtt
     4981 tcgataccta atgacaatgg tgtaacgtca agtaaaacaa catctttcac atcaccagtg
     5041 atcacgccac cttggatagc agcacccata gcaactactt catcagggtt aactgatttg
     5101 tttggttctt ttcctgtttc tttacgtact gcttctacaa ctgcagggat acgagtagat
     5161 ccaccgacta aaataacttc gtcgatttct gattgtgata aacctgcatc ttttagcgct
     5221 tgacgtactg gtacttttgt acgttctact aagtcagctg ttaattcatc aaattttgca
     5281 cgtgtcaatg tcatttccaa atgcaatggt ccagattctc ctgctgtgat aaatggcaag
     5341 ctgatttgtg tgcttgatac gccagataaa tcttttttcg ctttttcagc agcgtctttc
     5401 aagcgttgta aagccatttt gtcttgagac aagtcaatgc tgttttcttt tttgaattct
     5461 gctaccatgt agtcgatgat tttgttatca aagtcgtccc cacctagatg gttgtcgcca
     5521 gctgttgaca atacatcaaa gacaccgtct cctagttcaa ggatagatac gtcaaaagta
     5581 ccgccaccaa ggtcgaatac taagattttt tcgtcgcgat ctgttttatc taaaccatag
     5641 gcaagagcag ctgcagttgg ttcattgacg atacgttcta cttctagacc agcgatctta
     5701 ccagcatctt ttgttgcttg acgttgtgca tcattaaagt aagctggcac agtgataact
     5761 gctttttcga ctttttcacc taaatagtct tctgcgaatc ctttgatata ttgaaggatc
     5821 attgctgaga tttcttgtgg tgtatatgat ttaccttcta catctacttt gtaacctgct
     5881 tcaccgatat ggcgtttgat tgatgaaatc gtatttgggt ttgtcacagc ttgacgtttt
     5941 gcaacttccc ctacttggat ttctccattt ttgaatgata caacagaagg tgttgtacgg
     6001 tttccttctg ggttagcgat gatttttgct tcgtttcctt ccaatactgc tactgcagag
     6061 ttagtcgtac ctaagtcaat accgataatt ttactcataa ttaacgtctc cttctttaat
     6121 tgaaagtgta agttttatat ccgcacaatt attgtgcaac aatgaccatg gttggtctta
     6181 acacgcgatc atgtaatttg tagccttctt gtagtacttc tacaatcgta tcggctggtg
     6241 tatcttcagt cgctggaaca gtttgtactg cttgatgaag attaggatca aaggcttctc
     6301 ctttagcagg gattttttca attccttctt cttccagtgc gttacgcaaa ctttccaaga
     6361 ccatttccac gccttttttc aaacctgccc cttgatcatc tgacacttct gtggctaatg
     6421 cacgttccag gttgtcaatt gatggaagta attttttggc taaatcttgt gaacggtatt
     6481 tttgtaattg ctcacgctca ttttttcctc gatttgccat atttgcgatt tcagcacgag
     6541 cccgcaagta tttatcttcc atttcttcta actcagcttt taatttcgct gtttcgaatt
     6601 cttccgcttc agcttctgaa atacccgaat cttctgcagc ttcgacatcg atttccggtt
     6661 ctggctgagc atctgtcatt tctttatcta gttcttcttg attttctttc acgatacagt
     6721 cttcctttca ttattcagtc agctgtttga ttgctcttac taagaaagag agaacgatca
     6781 gctgcaaata gcttcgttat tcacattacg agtttgacaa gtcaagcgaa cggtagtaat
     6841 cagcaagttt agctgctagt tctcttcgat aaacatcgac tagaccgaac atccgagaat
     6901 aaggcatact agtaggccct aacaaggcta ttgtacctct tccatgacca tctatctcgt
     6961 aacttgcttg aatcatactc atattctgca ataaatcatt gcctaattca gcaccaatct
     7021 tgatatgaat attgctatcc atcggagtga tcatttgtgt aagtccgtct gagtctttca
     7081 taaaagaaaa catcgatttg aactgatcaa gatcttgacg gggctcataa tccaagagat
     7141 tcatttgtcc actgacataa accttatctt caaaaacatg accaagaacg gtgtcaaata
     7201 actgagacat cccttctgtc gtttggaaat atttatgaag aatcatcgga atctccgtac
     7261 gaagtttatg gtacaccgtc atcagaggat ctccgactag acggtcgttt acaatctgta
     7321 ccattttttc tagttcctga cttcccatat attcgggaag agtgaacact tggctttcta
     7381 cattccccat atctgttacg acgatagcaa taatctgtcg attattcaag ggtacgatac
     7441 gaaagccagt cagtcggcgg tcttttattt caggtcccaa tgaaaaagtc gtatagcttg
     7501 tcagctcaga taatatctca gcagattgct taataatctc attgatttca tgaaattctc
     7561 tgcctagaga ctgacgaatc agcagcaaat catcttctgc tacgcgagca ggttttaaca
     7621 aatgatccac gtaataacga tagcctaaag cagatggaat ccgcccagaa gaagagtgag
     7681 tcttcaacag caatccttca tcttctaatg ctttcatatc attgcgaatc gttgctgagc
     7741 tggccttgac tccctcagac atcaatgttt tggaaccaac gggcacgcca ctactcgtat
     7801 agtgttggat gatcagacgt aaaatgtcgt tttgtcgctt tgttaacatc ttcctcacct
     7861 ctcattagca ctcatatatt ctaagtgcta atccacttat aaatatagca agaagccctt
     7921 ctcctgtcaa gagaaagact ccatttttta gcactctaaa cacaagagtg ctaattctaa
     7981 ataattgatg taaatccagt tatatcaacg tttcaatagt cctttattgt atttttgaac
     8041 tttttttatt taaattcaga taaaaaacaa atattcttta aactattttt caaacaaaaa
     8101 gcgctcaaac acattgttcc ccaaaaaggt ccctcttggt gtcaaataaa cacggtcttc
     8161 ttcattaaca agtaaccctt cttgctctaa ttcccttagc acatcaccgt acaccgattc
     8221 aattgttttt tgatagcgat catagaaatg ttttttagta atccctgttt ttttcttagt
     8281 cctaagaaca tttcttcttc tatttgattt tttcgtgtca atttttcttc ttccaatacc
     8341 gggcgttcat ttgctcgtaa tgggcgtaaa tagtgttgga ttggtccttt gttgcggtaa
     8401 cgaatatctc caagataacc gctagcacca gctccaaacc caaaatagtg gtcattgttc
     8461 cagtacatga ggttatgttg agattcatgt ccggagagcc caaagttact tatttcatat
     8521 tggtgacgtc catgcttttc cattgcttca atcgtctctt caaacattct ggtttccgtt
     8581 tcctgatcag gcagttcgag tcttccttgt cgtacccagt tcatgaacat tgttttgttt
     8641 tcgaggatca atgagtacaa agaatagtga ggcagatcca aagccaaagc tttttccaaa
     8701 gtatccagat atccttctag tgtttgtccc ggtaatgcat aaatcaaatc gatgcttaca
     8761 ttggtaaaat tttctgcttc taaaaattgc atagtttgat acacatcttc tgcggtgtgt
     8821 ttacgtccaa tttttttcaa taagcgatta tcaaatgtct gcactcccat ggataaacga
     8881 ttgacgccat agtttttcat tacttgaagt ttttcacggg tcaaatcacc tggattcgct
     8941 tctacagtaa actcattacc attttgaaaa ggcaggatct ctctagcacc tgataacagt
     9001 cgatcaagtt gtttagcaga taaagaagtt ggtgtcccgc cccctacgta aagtgtttct
     9061 aaattcgctg tcggatgact ttctagcatt attttcattt cattcagcag catttccaca
     9121 tactcatcta ctggctgccc ttctaaaaaa actttattaa aatcacagta atagcagata
     9181 tgctcgcaaa atgggatatg aatgtacgca gaaaccttgt tttctgcggg atttattgtt
     9241 tctatcattg aaattctttc tccttacgtc gaaaagtcgt gcctgttttc gaccttctta
     9301 aaatcgcgtt caagcaccaa ctcattgaaa taagccgcat atttagaatc aaggaaacct
     9361 agctctaaaa atgcttgctt attgtcatcg gagctacacg gtgcttttac agcctctcga
     9421 taaacggtaa tcgcaaagct gaccttcggc attaagtcaa aatctgcaaa aatatgaaaa
     9481 gctattttca cagattcctc cttaagtgct cagaccacaa cgctttgctt acaacctctc
     9541 atatacgcat actaaacgta tttggcgaac aaatccacta aaaaatccaa gaatcgactt
     9601 cttggatgat tttttctttc attaaactgg ttacctagtt atatatctct agggttgttt
     9661 tcatgtctga gtgcccgata tgatttattt ttcgtttcta taattacttt ttgccttcca
     9721 gtttctttta atattgtact caagcaagtg ggaattggtt cttttgtcat ccatgtatgc
     9781 aaaatgttat aatgctggta aacaaaaatg attacctgtc atttttattt agttcgtatt
     9841 taattaagtt gactatctgt atactctgtc taaaacttaa gaattgttgt tttcgctgtc
     9901 gctacccaat tttaatgaat gtctgtaggt taaaactgtt gtccttgtta tagatagtaa
     9961 aggagagctt gaaaatgaaa actgcgatta cgtttctatt agaggaatat gccgattggg
    10021 aaggtgctta tattactagc acactaaacc aaaatgagaa atggcaaaac aaaactgctt
    10081 ctatccaatc agaggtcact tccattggag gattaaaaac aaaaatcgat tacttaatcg
    10141 aacaaatacc tgaaaaagtt gaccttttta ttatgatcgg tggcaattca tgggacataa
    10201 aaaatcatca tttatatcaa ataatcgata aattgttaaa aacagacgta ccagtagctg
    10261 ctatttgtgg ggcagttgat tatttagctc agagcggatt attgcaaggt tacaaacaca
    10321 ccggaaatgc gcaatttcct tggttagaat cggaaaatta tcagaataaa gaagatttta
    10381 tagaacaaca agctgtgaaa gatcgtaatt tgattactgc gaatggaaca gctcctatag
    10441 aattcacaga cttgattctt caagcagtcc attttgaaac agaagaaata agagaaaaaa
    10501 cgattcgtct atacaaactt ggattctatg attactataa aaagtacgga gatcctttct
    10561 cctaatcatt ctcttcaatt aaatactcct tatcaatagt gtgacaagta attttactga
    10621 tgattatttg tcacactttt tctgacattt atcaaagaag atacactcta tttatattca
    10681 tagacaaggt tttacggatt caaaaattca ggattatgct atactggtat taaaaaaata
    10741 atgaggtatt ttatatgaaa aagttaggtt ttattttatt gttggtctct ttattccttg
    10801 taggatgtac gtctcctagt aaagaaaata gtgatccatc tgcgaccaca gacaaaagtt
    10861 caatcagaca aaccagttca gctgaaaaat caagtactca gaaatcagct tctactgaaa
    10921 cgaatacatc ttcaacaagc agtaaagtct cagagcccaa tacttcttct acatctagta
    10981 gtacagttga gacaaatatt tcttcttcaa caatagacac aacctcctca cagtcttctc
    11041 agtcaataga tccgttagca ggatattctg atctacaaat tgagtacgca cgtgtttggc
    11101 tagctattag aggtaatact tttagagata catcgggtga tttcgaatta catgttattc
    11161 attatcctgc aggaacacct atcgcccctt atgatgcagg gagtgcagtc tatccgaaag
    11221 atgtggttat gctcactggg aaatacagtt accagggagt tttagtgtat tcaagtaacc
    11281 atgacgggac aatcactagt tatccagttc cttcacattg gcaaatgcct gcagatcaaa
    11341 catcttatcc agcgttcatt agagaaacaa ctcaagaaat cattgataat gcatctgtag
    11401 tagctattcc tacaggaaat cccaatgacg tgaaacagtt aatcgatgtg acagttatag
    11461 ataattagac tttctaatat gttatacaaa aagaagctga cagc
//
LOCUS       DAZKOU010000090        11337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000090 DAZKOU010000000
VERSION     DAZKOU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.091"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(364..1482)
                     /locus_tag="ACG827_002335"
     CDS             complement(364..1482)
                     /locus_tag="ACG827_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484454.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1715..1837
                     /locus_tag="ACG827_002336"
     CDS             1715..1837
                     /locus_tag="ACG827_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484455.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1953..3260
                     /locus_tag="ACG827_002337"
     CDS             1953..3260
                     /locus_tag="ACG827_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0484456.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="ACG827_002338"
     CDS             3379..4578
                     /locus_tag="ACG827_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0484457.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="ACG827_002339"
     CDS             complement(4605..5873)
                     /locus_tag="ACG827_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0484458.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5928..5994)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6036..6682)
                     /locus_tag="ACG827_002340"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="ACG827_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="ACG827_002341"
     CDS             complement(7090..7593)
                     /locus_tag="ACG827_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0484459.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="ACG827_002342"
     CDS             complement(7716..8480)
                     /locus_tag="ACG827_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0484460.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="ACG827_002343"
     CDS             8588..8863
                     /locus_tag="ACG827_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0484461.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="ACG827_002344"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="ACG827_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0484462.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10430..10609)
                     /locus_tag="ACG827_002345"
                     /pseudo
     CDS             complement(10430..10609)
                     /locus_tag="ACG827_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3401 a         2387 c         2002 g         3547 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg
    10381 atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa
    10441 cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc
    10501 cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc
    10561 ccttcgccca tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga
    10621 taccacccat ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca
    10681 tccctttttc tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct
    10741 tcttataaac cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg
    10801 ctcctttccg caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa
    10861 atttcttcaa atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg
    10921 gtatttaaaa atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta
    10981 aagacactct tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac
    11041 ttatcttatt aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac
    11101 cggacaacta tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga
    11161 caatgaaaaa agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac
    11221 tgctcttcga accattctca gtgcaatccc taatttatct attaaatctt cttttagttc
    11281 ctcatctttc atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaata
//
LOCUS       DAZKOU010000091        11231 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000091 DAZKOU010000000
VERSION     DAZKOU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.092"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..329
                     /locus_tag="ACG827_002346"
                     /pseudo
     CDS             1..329
                     /locus_tag="ACG827_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(327..1046)
                     /locus_tag="ACG827_002347"
                     /pseudo
     CDS             complement(327..1046)
                     /locus_tag="ACG827_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(997..1206)
                     /locus_tag="ACG827_002348"
     CDS             complement(997..1206)
                     /locus_tag="ACG827_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484463.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(1188..1256)
                     /locus_tag="ACG827_002349"
                     /pseudo
     CDS             complement(1188..1256)
                     /locus_tag="ACG827_002349"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            1483..2756
                     /locus_tag="ACG827_002350"
                     /pseudo
     CDS             1483..2756
                     /locus_tag="ACG827_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2863..3894)
                     /locus_tag="ACG827_002351"
     CDS             complement(2863..3894)
                     /locus_tag="ACG827_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484464.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3901..4731)
                     /locus_tag="ACG827_002352"
     CDS             complement(3901..4731)
                     /locus_tag="ACG827_002352"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0484465.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4728..5543)
                     /locus_tag="ACG827_002353"
     CDS             complement(4728..5543)
                     /locus_tag="ACG827_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0484466.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5540..6364)
                     /locus_tag="ACG827_002354"
     CDS             complement(5540..6364)
                     /locus_tag="ACG827_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0484467.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6354..7454)
                     /locus_tag="ACG827_002355"
     CDS             complement(6354..7454)
                     /locus_tag="ACG827_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0484468.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7632..8417
                     /locus_tag="ACG827_002356"
     CDS             7632..8417
                     /locus_tag="ACG827_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0484469.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8450..8833
                     /locus_tag="ACG827_002357"
     CDS             8450..8833
                     /locus_tag="ACG827_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0484470.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8909..9040
                     /locus_tag="ACG827_002358"
     CDS             8909..9040
                     /locus_tag="ACG827_002358"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0484471.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9009..9245
                     /locus_tag="ACG827_002359"
     CDS             9009..9245
                     /locus_tag="ACG827_002359"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0484472.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            9257..9343
                     /locus_tag="ACG827_002360"
                     /pseudo
     CDS             9257..9343
                     /locus_tag="ACG827_002360"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(9323..10294)
                     /locus_tag="ACG827_002361"
     CDS             complement(9323..10294)
                     /locus_tag="ACG827_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0484473.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            10946..>11231
                     /locus_tag="ACG827_002362"
     CDS             10946..>11231
                     /locus_tag="ACG827_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0484474.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         3338 a         2593 c         2145 g         3155 t
ORIGIN      
        1 actcagatgt tgaaagtcaa agaaaagttg actattttag aattaaaacg tacccgtttt
       61 ctatgctatg attgtgggca gaccttttct gcgaaaacgg acttagttga tgaacatcat
      121 caactaacaa aggaactgaa gcaggcgatt ctcatagaac tgtacgaaaa tcaatcacgt
      181 aatctgaaaa tcagaagaag taccgtcggc tcaagcgata ttggaaactc ttactcaaag
      241 attccacgac attggagccg ctcaaacggc attatcatcg actgtttaaa cgaccaatta
      301 gtcaaacaga aatcgtcgat gaaccgttgt gcacaatggt gtcctgaacg tacagcgaca
      361 ccttccatat ccagagcagt cgccacatca cggggatgga tgccatccaa attaaaagca
      421 accacgccag tatgatgttc aggatcttgc ggtccgtaaa tggtgatacc gccgatcgct
      481 tgtaatttag gtaaaacgta ggcaaccagt tcttgctcat aacgatgaat attctccata
      541 ccaatctcat tcaagtaatc gattgctcct gcaatgttcg gagtaccagc ttcaaatttc
      601 catggcagct ctttccatga actatcgtaa agattgacaa aatctatcat ttcaccgccg
      661 aattcgactg gctccatttg ttctaacaat tgacgtttgc catataatac accgattcct
      721 gtcggcccgc acattttatg cccgctaaag gcgaaaaaat ctgcatccaa cgcttgtaca
      781 tcaactgtca tatgcggtgc tgcttgggca ccgactggac tatcctcaac tacaacagcc
      841 tgttcaaccc tcagccgttt caagcacaac aaataacctt ctggatctgg tttgccgacc
      901 tgaagatcat cgatcgtcac aaaggaatca attttttcct taatcatcgg atgtgcttca
      961 aaaaaatagt ctagctcccg cagttcagaa ttgagactac tccgattttc cagtgctgct
     1021 gatccgctgc tcaaaatgtt cagcatccac tggtctccca accgcttgac ttatcccttc
     1081 ataaaaggac agttctgctg ccgtcccaac accttgttga tagtctgcaa aaggaatttt
     1141 atagccgaac tgctccagta aataggcttc agtaaatagt gatgaatact ctcgtaaaac
     1201 atccattcac tgtcaatcat cgtcccatca aaatcaaaaa taagtgcttt ggtcatcttc
     1261 tttccccctt acccaaaaca agcaaaagct gtgtcctgct ctctctacag tatcagcata
     1321 aaaactgccg tctgaaaagt caaaggtgaa agaaatgtgg cgctataatt tctaggactg
     1381 atagtaaatc attctatcag tccttttgag cataaaaaaa caaaacatac agaagctccg
     1441 ttagaattga atcaccacaa cacaatcaaa ggagcgttct gtatgcctga tcttcatctt
     1501 atcaaactat tactaggaat caaagatgga aacattcaaa tcactcatgt cgagaagaaa
     1561 aaaatacatt caatcaacag tatcgttgtt tctgccacgc tcattaaaac gattcagccc
     1621 tgtcctcagt gtaaacaggt gaatcaacat gggctgatcg ttaagaacgg aaagaaaaaa
     1681 tcgctggtcc ttctcaataa gtgtgcgaat caacggactt atcttgcttt ggcaaagcaa
     1741 cggtacctct gtcgtggctg tcacacctat tttacggcga atacctacat tgttgaaaaa
     1801 aactgtttta tcgccaaaca agtacattat aaaatattgg aggaattgac tgaaaaacaa
     1861 gcaatgacga cgatttctaa acattgcggc gtttcttggt caaccgtttc gcgaacactc
     1921 gcttctctgc tccccatgac aaacgtgaaa ataaattggt tgcctcgctg ttcattggta
     1981 gatgaatttc gttcattgaa aaatcaagtc ggtccctatt ctttttcttg tatggatggc
     2041 gatactggga aattactgga tattcttcct tcacgtaaga agaaagatct catttcttat
     2101 tttatgcaat ttgaacgacg tgcacgttta aacgtaaaaa tactcgtaac agatatgaac
     2161 gcctcttatg ctttgctgat aaaagagtgc tttcctcatg ccaaactcgt tgtggatcgc
     2221 tttcacatcg tcaagcacct cattcgcagt tttaaggata tttgactatg agtcatgaaa
     2281 agcttcgacc gaaacgatcc aatacaagcc aaacactatc gacaagtgaa ggctttatct
     2341 cgacttctga tcaaacgaca ggacacgctt gtttatgata aatggacaaa atggcgtaat
     2401 tttggttggg cctatttaac cgaaagtgaa gtcgtagaac gtctactttc tacttctgat
     2461 gaactacgaa tagcttatgc ttactatcaa gaaattcttc aagcttttta tgacaaagaa
     2521 gcggacatct tctttcagct tgtaaaaaca atgcccaagt cggttccaag agaactgcat
     2581 catataaaaa aagcgtttat aaactatgaa tccggcattc gtttagcctt agagttgcct
     2641 tactcaaatg gcaaaataga aaatctgcat acccacatca aagcattgaa acgaatagct
     2701 tacgggttag gatgagaacc agaattttct taatcaatca tctgatcact tattaaacat
     2761 aaaaaaacat ctaaagcaga aaatcaattc taccttagat gttcttatat cactctatca
     2821 gtcctatttg acaaagagcc agaaatgttt gccgactata cactagcttt gttgtgcaaa
     2881 atgctgagta tcaatttcca cagggattgc tagaatatct ttcagctgaa tgtataaggc
     2941 atcgctaatt ttctcttccc gatcgttcaa ccgatccact aaaatttctt tgccctccag
     3001 cgtttgaaaa acataccgtt gaatgttgcc taaaataaat gattccttcc atagaaccgg
     3061 aatcttaacc ccctgaggaa ccggttcaaa ttgaatcagc tccggtctta aataataaat
     3121 gcagcgattg cccttagcgg aaaactcgtg ggtatactga ttcagctcat accattcgaa
     3181 acgattatag ttgccgataa attccgcaac aaatcggctc tgaggattct gatacaattc
     3241 cttcgaggtg gaaacctgag ccacccgccc agcttccatc acaaaaatcc gatcactgat
     3301 tcgcatcgcc tcttcttggt catgagtcac aaaaatcatc gtcatcccta gcgattgctg
     3361 gatagcgcgc aaatcccgct gcagctgttt tctaattcgg gcatccaaag cacttaatgg
     3421 ctcatccagc agcaggactt tcggttcagt gaccaatgag cgagccagtg ccacccgttg
     3481 tcgttgaccg ccagaaagaa actggacatt tttatctgcc tgctccgtca tatccaccaa
     3541 tgatagcatc tcaaaaacct tttcctgaat cacctcagga ctcagcttct ttaccttcaa
     3601 accaaaggct acattttcaa aaacagtcat agttggaaac aatgcatact gttgaaaaac
     3661 aaagccgata ttgcgatcct tcgtaggctt atcggcaata ttctcattgt ccagcacaat
     3721 ctcaccgctc tttggttgtt ccaatcccgt gatacagcgc agcaacgtag actttccgca
     3781 cccgctggga cctagtaaag taaccagctc acccttttca atctgaattt catcaatagc
     3841 taaaatctgt ttttcatcaa aaaacagatt gagatcatgt atttctaaaa acattttccg
     3901 tcacaactcc tttaagggct tctttttcga ctgcttctct tccctcgtct gcttaaggga
     3961 cagctgttgc ttttgtcgat aagtcagcat aaaggtagtt aaagcaattc ccaccataaa
     4021 cagcaaatac aaaaccatca cagcgctagc caaatggccg ttttcgttca tccgccgcag
     4081 catatagatg cgtaccgtct gaaatcgacc gccaatcaac aggttcgtca gcatgtactc
     4141 cccaaaaacc gaagaaaaaa tcatcagaga aactagggtc acaccgatac ggatgtttgg
     4201 aaacagcacc tggataataa tcgtagacat cggggcgccc aaggtttctg ctgcatccac
     4261 taattccttc agattaatta agcgcatctg gttgttaaga ctaagataga caatcggcaa
     4321 cgccgtaata aacaatgcac ctaccaacac tacaaacatt gggataccag tttcagcata
     4381 cgttcgaagc aaagccgtta ccaaaatcac tcctggtaaa gcatacggca gcaggagcag
     4441 cttttccagc caacgattca aacgagggaa atacaaatga atccaaataa tggtgggaat
     4501 catcaacaac agaatcgtca ccaagaccac acctgctaat aaaagtgagc gacccaccgc
     4561 agcaataaat tccctgtccg tcagcagctg ttggaaccat tgcaaggtaa aatcactagg
     4621 taaaagcgat tttgaccatt tagtggagct ggcatacaaa atcgttccaa taatcggcag
     4681 caagtaatac aggacaaagc agaacaaaat cggttttatt aactttttca cgtctgcacc
     4741 accttgcctt tcttttgaat aatcaacatt gccgttacca gcaggacacc taaagcgact
     4801 gccaacgcac tgcctaaatt aggcttggcg aacacatctc ccgacactaa agccgcaatt
     4861 cgaatcgtta aaagatttac attactgctg gtcagggcat acgctgtttc ataggtcccc
     4921 atgccattag caaataagat gacaaaggtg ctggcaatcg atggtcgcaa aaaaggcaaa
     4981 gccaccttga accaagcata cgctgatgaa gcgcccagca attgtgcgga ctccagccat
     5041 tcctttttca cttccttcat gctgggatac aaaaacaaga cccctaacgg aatttggaaa
     5101 taggtatacg tgatggtcaa tccccaccaa gaatacaaat tgaagtccgc tagaaatggc
     5161 aaaatcattt tcagcacacc tacattcccc aacaaaataa taaaagagaa tgccagcgga
     5221 atcccagcaa aattagctgc caaattggaa acaaaggtga ttttctcctg caatttagtc
     5281 gatagattca ttaagcataa acataaaata aacgtcccga ttaacccaat cacactggag
     5341 ctgacagcaa tcagtacgct gttaacaaag gcctgtgtgt aatatttgtt gctgaaaata
     5401 tcactgtaat tggccaatgt ccactggtta gttaaatcac tgtgcaagct gcacagaacc
     5461 ataaaaagca gcggcagcag taaaaatgca cagaagataa aggaggcagg aaccagaacc
     5521 aagcgttttt tcatctcttt catcccctca tcaattcttg aatgtcctgc attttctctg
     5581 acggtgcaat tcctaataac cagcaaaaca atggagccgc cagcagctgt ggtacagttt
     5641 gttccttcag cttttggcgt ttatttgaaa tcaaataaaa aggaacttca cgatgtgcag
     5701 ccaagctgcc gccatgcagt ccgtgcgcat ccatcccgtg atccgcagtc accacgactt
     5761 gataaccttt ttgcagccat tcaggaaggt aaatagccaa taatctgtcc gcctgattaa
     5821 ccgctgctgc atattcatga gaatcggcgg taaatttgtg accgacatca tcaatattca
     5881 tcgaatgtac gaccaaaaag tctggctgat actgattcac taaataatgg gcatccgcaa
     5941 ataaatgcga atcagggtaa gtgtcctcac tataaaagat gccatgctga atcagtcgct
     6001 cctcctgatg ctggatgcga tcaaccaaac gattataggg ggcttgattg tacaactcgc
     6061 tgtaccagtg gtaagcggcg gctcctgttt ttccgccagc ttccttaact aattcaaaca
     6121 gcgaccactc tttggatcgc tgagcatggt aattactata gatgccattt tcataggctg
     6181 gaacgccggt catcagtact tcgtataacg gacgtgaatt gctgggcagc tcacttttta
     6241 ccttcaataa gcttgcttgc tggtgctcaa ctaaatgatt catgaaccca agctgggcga
     6301 cggctgtgtc gtagcgacag ccgtctagta taactacaat cagtttgtca agtctacttt
     6361 gcataggtca gtacctccgc ctgccaaagc tctggaagct ctacagaggt cttgttccaa
     6421 gcttcattgt cttcaactgg caccgccgct tcataagcat cctccggcag taatttttca
     6481 gctacttctt taggcagctc taccttgcga ataggtcgcg catagccctt tgccaaattg
     6541 atttgccctt catcagataa aatatattcc cgtgctagct ttgccgcatt gggatgaggg
     6601 gcatttttat tgattaaggt tgtatacccg ctaatgatgg agccgtctgt tggcacagtt
     6661 acctcaaaac gctcggagtc aatttggtca cgataattta gtccattgaa gtcccaaaca
     6721 actgccacat cgatttcacc tttttcaaga tttgctacgc tggcatcaac ggtggacaaa
     6781 cggccttttt ctgccatttt tttgtagtaa ttcagacctg gctgaatatc cttttcgtca
     6841 cctccgttgg caatcgccgc agccaataca gcaaattgtg cttggttggc aatcgtcaca
     6901 tcccccacgc taacttgata gtcaccctct tgcagctcct tccagctagt cggtgcttgc
     6961 ttcacattct ctttatcggt gataaaggaa atggttcccg tatagcttaa caaccaatcc
     7021 ccatcatcat cctttgccca atccggaatt tcctcccaat agttggtctt ataaggtaag
     7081 gttaactctt tgctttcagc caacggaccg aagctgatac cgacatcacc aatatcggcg
     7141 gttccatttt ttccttctga ttcaaactta gccaattctt ctgcactgga catatccgta
     7201 tccacgtgac ttaagccgta ttctttttca aggtcgttcc aagtgcctac ccaatttgcc
     7261 caagtatccg gcattcccaa gctggcaatc tctccttctt ccttggcttt ttctataatc
     7321 gtatctaaag agtcgtctgc cgcagccgat tgcttcgttg cttcacctga gccgcagcct
     7381 gtcagcagga tacttactgt tgctgcacaa aataaaccga tagtcatgtt acgaattttt
     7441 tcattcattt tcattcattc ttctcccctt tcgataattc cattctaaca aagctttgta
     7501 atgagagaat cagtttcttg aaaaggtttc atgaacaatg taaaggttgt atatggtttg
     7561 taaatttcga cgaattcccg ttaaccttct aaagcttttg cggtatgata gtagaaaaaa
     7621 ggaggctccc tatgcagtat tatgatcagc atctccacac gtatttttcc tttgattcag
     7681 aggaaaaatt tgaaaactat ttagcctatc agccggagtt tttcgtttct acggatcact
     7741 ttgatttaaa caatccatct accggaaaag atgacattcc ggattatcca gcctatataa
     7801 acaaactgga ctctttaaaa gaaacctatt ccaccaaatt tctgcgaggt atcgagattg
     7861 gcgttgttcc aggacaagaa gagcggatcg tggattacct cgctcagcat ccttatgatt
     7921 taaagctggt aagtattcat caaaatggcc gctttgatta tatggacgat gtggttttaa
     7981 ccaaagatcc cattcaaact accaaggaat attttgatca gatgttgcag gtcttgacgg
     8041 attttccaga aggagatatt ttgactcact ttgattatgg attacgccgc ttcaccttca
     8101 cagcggaaga attcgcggag cattttgagg ctcagctgct aacgattttc aaaaaggttg
     8161 ttgaacgaga attggcgatg gagttaaatg ccaagagctt cttgaaatac ggcaacgaag
     8221 acctttaccg ttatgccgtt cctttgtatc aaagtgtcgg cggaaagctc tttaccttag
     8281 gctcggatgc tcatgttgcc gaagattatc agctgggctt taaaaaaatg agtcagctgt
     8341 tggcagactg tcaggtagaa aagttgctag tcattcaagg aaaagaacgc cgcctgacgc
     8401 cgctgccgat tttgtgattc ctatataata gaagaaagtg gtgaaaaaaa tgagcaacct
     8461 gtgggactac aatcaagagg cacccatcca ttatttaatt gcgcgacatt gggatgcgtt
     8521 gaaaattgag gcggtttgcc gatccttgtt agcagccgtt ccaaaacaac aactggaaaa
     8581 ttttttggtg gctgacagct tgcagcggga aaaagttcag gcttattttg cagcctttaa
     8641 agatcagcct ttggaatatt tgcatgcgca gtttcatctt ttttatcaag tagccgcccc
     8701 agatgactac aacgatttac gaggacagct gcaattgacc tttcaagcgg atgaaaccgc
     8761 ctataccgtt ttattgggaa tggctcgcct tggtgatcag gcgaaggtcg agtggcggat
     8821 tttcgatatt tgatggcagg atcaaggcgt ttcagttgca aaccttcctc tctttcaatt
     8881 agaaaaaccg cagcaggaag aagtttgtgt gctggtcacc gatgctggtg aaattcgtct
     8941 ccgcttattt cctgaccaag ctcccctggc tgtagccaac tggcgctccc tagctgagca
     9001 aggcgtttat gacaacacac ctttcggctc gtgtcattaa ggaatttgtt attcaaggtg
     9061 gcgctttgga tggttctggt gatgaagctg tttccagcta tgagggcttt ttcgctgatg
     9121 aagtgcatcg cggactttat cattttaatg gcgccttggc cttaggggac cacggccccc
     9181 acaccaacgg caaccagttt tttattgtgc aaaacacaaa ggcgcaggct gacctgctaa
     9241 tgtaaagcaa gcatacttgg agcttggagg actaccggag ctggatggtc gttatactgt
     9301 ttttggacag gtataccaag ggttagaaat cgtagcagcg atcgcagctc agccgacaaa
     9361 cagtgaagat gtaccgattg agccagtgtt cattcgacaa attaacttcg aaaaaacatc
     9421 gtaaaaagac actcactgac ttcagcaaaa gctctattct tgagctggct gcagctagtg
     9481 agtgtctttc atttatccat aagataaagc ttggattcaa taaataaata aatgttttgg
     9541 ttaaagctgt gaccagcaaa tgaggaaccg tgcgagccag ttgatctgcc aatgcaaaca
     9601 tttctttagg tgaaggcgct ggcgtgtctg ccaacggata ctccagtccc aatgcttggt
     9661 aaccgtgcat accatagcag tggtacggta gtaattctaa cggtacgaac cgatcaagat
     9721 ctgccacaaa acaagcaatc gtcagcagct cggtcaggtt catattgata cctggaacca
     9781 caggaactcg aattcgcact tcgcagttag gatactcaat taaaaagcga cgaatattct
     9841 tcaaaatttg ttggttatct aagccggtcc attgtttgtg gagcttgctg gtaaacattt
     9901 ttacatccac aaacaatgta tccacatagg gagctacctt ttggatttca ttataagcac
     9961 cacaaaagct ggtttccagt acggtattga ttccaatgta tttcgatttt tgcaaaattt
    10021 ctttagcaaa atccgcctga accagtgcct cgcctccgct aatggtcact ccgccatccg
    10081 agtgaaagta aaaaacagca tccttctcaa tctcatccat tacctcttca gcggtcatgg
    10141 tctggccaaa tcctttttca atctgcttgc tttgtgattc gggaatcgaa caccaggcac
    10201 agcgtaatgg gcagcccttt aaataaacaa cggtccgtaa accagcacca tcatgtagag
    10261 aaccctgttc tattcttaat actgttcctt ccatatagat ttttgaatta cacactagtt
    10321 taaactaaaa cacacaagga aaaacttacc atttaatcat aaaattagct catataaata
    10381 tactaggatg taaaatagac tggtcaaaat ctggtcaaaa aagaggacta atacaggtag
    10441 tcctcttttt tgttatttta atattctaaa tcatttttta ttggtttatt cccttattcg
    10501 atgtagactc aaatcactat atcactagat atcaaacggc gtcatacgga atcaaaaaca
    10561 tcaaatttga cctgttaaat ggtcattgaa taacattcaa aatcaataaa acggagtcag
    10621 acacggagtc aaaaacgctg agagtaaaaa atatatacag ccttgtaggt aaagtaccac
    10681 agggctgtat attttttatt tcagattacc ttaaatgatt ccatttactt tttatctcat
    10741 tttttcctac tagttagtat caaatactct cctattttct caataaacta tttttggata
    10801 catggtatct gaaacagcta aaaccttgtc attacagcaa aaaatcacgt tatccagact
    10861 gtagacaaag tccattttat ggatgatgtc tacagtcttt tcttttataa tagatttatg
    10921 aaaacgaatg caaggagtga tagctatgat gtctaaaaac aatacgaatg gtagaaatca
    10981 atttgcgatg cttacgattg atgatctagt tcctcaagat catctcgttc gaaagattga
    11041 tgccgcgctt gattttgaat ttatttatcc aatcgtcgaa gccacttact ccgatttagg
    11101 tcgcccaagt atcgatccag tgattctgat caaattggtg tttattcagt atttattcgg
    11161 tatccgttcc atgcgacaaa ccattaagga agtcgatacc aatgtcgctt accgctggtt
    11221 tttaggctac t
//
LOCUS       DAZKOU010000092        11197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000092 DAZKOU010000000
VERSION     DAZKOU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.093"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG827_002363"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG827_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0484475.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG827_002364"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG827_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0484476.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACG827_002365"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACG827_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0484477.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG827_002366"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG827_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0484478.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG827_002367"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG827_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0484479.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACG827_002368"
     CDS             complement(6343..7062)
                     /locus_tag="ACG827_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0484480.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACG827_002369"
     CDS             complement(7059..8372)
                     /locus_tag="ACG827_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0484481.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACG827_002370"
     CDS             complement(8453..8773)
                     /locus_tag="ACG827_002370"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0484482.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACG827_002371"
     CDS             complement(8881..9168)
                     /locus_tag="ACG827_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484483.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACG827_002372"
     CDS             9295..9939
                     /locus_tag="ACG827_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0484484.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACG827_002373"
     CDS             complement(9998..10705)
                     /locus_tag="ACG827_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0484485.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACG827_002374"
     CDS             complement(10925..11095)
                     /locus_tag="ACG827_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484486.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1956 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaac
//
LOCUS       DAZKOU010000093        11132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000093 DAZKOU010000000
VERSION     DAZKOU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.094"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1353
                     /gene="lysA"
                     /locus_tag="ACG827_002375"
     CDS             94..1353
                     /gene="lysA"
                     /locus_tag="ACG827_002375"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0484487.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1340..2140
                     /gene="dapF"
                     /locus_tag="ACG827_002376"
     CDS             1340..2140
                     /gene="dapF"
                     /locus_tag="ACG827_002376"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0484488.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2143..3024
                     /gene="dapA"
                     /locus_tag="ACG827_002377"
     CDS             2143..3024
                     /gene="dapA"
                     /locus_tag="ACG827_002377"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0484489.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3021..3737
                     /gene="dapB"
                     /locus_tag="ACG827_002378"
     CDS             3021..3737
                     /gene="dapB"
                     /locus_tag="ACG827_002378"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0484490.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3743..4438
                     /gene="dapD"
                     /locus_tag="ACG827_002379"
     CDS             3743..4438
                     /gene="dapD"
                     /locus_tag="ACG827_002379"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0484491.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4454..5455
                     /locus_tag="ACG827_002380"
     CDS             4454..5455
                     /locus_tag="ACG827_002380"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0484492.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5452..6813
                     /locus_tag="ACG827_002381"
     CDS             5452..6813
                     /locus_tag="ACG827_002381"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0484493.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6773..7924
                     /locus_tag="ACG827_002382"
     CDS             6773..7924
                     /locus_tag="ACG827_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0484494.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7899..9068
                     /locus_tag="ACG827_002383"
     CDS             7899..9068
                     /locus_tag="ACG827_002383"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0484495.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9243..10622
                     /locus_tag="ACG827_002384"
     CDS             9243..10622
                     /locus_tag="ACG827_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0484496.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10903..11132
                     /locus_tag="ACG827_002385"
                     /pseudo
     CDS             10903..11132
                     /locus_tag="ACG827_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3705 a         1945 c         2494 g         2988 t
ORIGIN      
        1 aaattttgtt taatattatt ccaataaaat tcaaatcttt atcaaatgaa attccaataa
       61 aaacggaaaa aaagtgaaaa gaggaatgaa aaaatgacag aattgataat tggcggagtc
      121 acagcaagca cattggcaga aaagtataag acacctctgt atgtgtatga tgaagaaaag
      181 atgagacaaa cgatgctgtc attcaagcga ggctttgtat cgaaacgttt tgatacaaaa
      241 gtgctttacg cctctaaagc attccaaacg attgagatgc tgcatttagt tcgtgaatat
      301 ggattcgggc tagatgttgt cagtggtggg gaaatctata ccgctttgca agctgatttt
      361 cctagagata ctatctactt ccatggtaac aacaagacgc cagaggaact gatctatgca
      421 cttgaaaatg atttgttgca tattgttgcc gataatttga tggaagttga attattagca
      481 gaattaagcc aacgctattt aaaaaaaatc tgtatcatgc ttcggttgaa tgtcggagta
      541 gaagcacata ctcatgaata tatcgtcact gcgcatattg attcaaaatt cggcatgagc
      601 tatgaaagta aagagtgcca acaagccctt cagttgataa aagaaagcag ctatctagag
      661 ttagaaggat ttcatgctca tatcgggtca caaatttttg atgttactgc ttggttagct
      721 gaaatcgaca aattgacgaa ttacttaacc gattttgacc aacctctttc cttgaatctt
      781 ggcggagggt ttgggattcg ttatacacaa aatgatcagc cgatcccagt cgaagaggca
      841 ctaaaaaaac tgattgaata tacggaagaa gctttggaaa aacgaaaact gaccattcgt
      901 caattactga tcgaaccggg tagaagtatt gttggagaag ctggtacaac actatacacg
      961 attggtttca tcaaaaagac accacataaa aaatattact ttgtagatgg cgggatgaca
     1021 gataatatcc gtccagctct gtatcaggca gaatacgatt gtgatttagc agcaaaactg
     1081 tcggaagaaa aaacagagaa agtgacgatt gcaggaaaaa tgtgtgaatc aggcgatgtg
     1141 gtaattaaag aagcttattt gccgaaagca gaatcgggtg atcttttagc tgtttattcc
     1201 acgggagctt atggatactc gatgagcagc aactacaatc gagcgacacg accagcagtt
     1261 gtttttgtca ataatggcca atcaagactg attgttcgca gacaagcatt ttctgatcta
     1321 ctaagggggg aagtttccta tgatccttca taaatataat ggctgcggaa atgattttat
     1381 cttattagat tacgcagaag acacagatta tagtcaatta gccgcttctt tatgcacaaa
     1441 agaaaaatat gatatagacg gattgatcgc cgtaaaaatc gatccgttgg aaatgatcta
     1501 ttacaacaaa gatggcagcc gcgcgccgat gtgcggaaat ggcattcgct gttttgctcg
     1561 atacgtaaat gatcagaaca tgatgaatga acaggaattc aacgtagaga cattagcagg
     1621 cacaagaaaa attacggtgt tggatccctc acctttttat tgcagcgtag atatgggagt
     1681 gccagatgat tctccggaaa aaatcaaagc taatcaaact tatccaataa aaaaccagga
     1741 aatagagatc aatggtgaaa aagtaacgat cactagttta tttttaggaa cgattcatac
     1801 cgttgttttt gttgaaaatg cgcgtgagga agtctggaga aaaagaggtg aactgatctg
     1861 tcatcatcct ttgttcaaag aaaaaaccaa tgtcaacttt gtagaagtag tcaatgaaca
     1921 agaattgatc gtccgtacct atgaaagagg agtaggttgg acacttgctt gcggtacagg
     1981 ttgctgtgcc tcttatgtgg ttgctaagga aactgggaaa atttcagcag atcaggtagt
     2041 tgttcacttg gaacaaggag acttgatgat ctctggtgag gatgcgatac aaatggctgg
     2101 cccagcagtt catgaatgga caagaatatt ggaggaatga agatgataaa aggttcgatc
     2161 gtggcgctga tcacgccgat gaatgaagag ggatcagtag attatgctgg tttggaaaaa
     2221 ttgatccaat tccatttaga tgaacaaaca gatggattat tagttttagg tacaacagga
     2281 gaatcttcta cgctcacaca atctgaggag gagcagatcc ttcaactgac agtaaagaaa
     2341 gtggctggcc gtgtgccggt gattgcagga gctggaacga ataatacgaa agaaacgatt
     2401 gaaaaagcga agcattttgc ttctttgggg gcagacgcac ttttggtcat caccccttac
     2461 tacaacaaaa caagtgatgc aggactagct gctcatttta cagcaattgc agaagcctca
     2521 ccgattccgc ttattttgta taatgttcct tctagaacag gcatgtcgat tccaatccat
     2581 gtattagtaa atttagcaga acatcctaat atcattggtt tgaaagaagc ctcaggagat
     2641 atggcttatg tgatggatgc cgctcggttg attggagaag aattcttcct ttacagtgga
     2701 aatgatgatt tgatcctacc agtaatgagt gtaggaggaa gcggtgtaat aagtgtatgg
     2761 gcgaatatcc aacctaaaat agtccatgaa ttagtaaaag atacgcagga cggaagatgg
     2821 cagcaagcaa aagaaaagca attaaatgca cttgaactga tccatgcctt gttcagtgag
     2881 accaatccta ttcctgtcaa agcagccatg tcattattag acttaccttc cggacctcta
     2941 cgcctgcctt tagtttctct ttcaaaagag aaaaagaaac aattagctca gcttttgtta
     3001 aaagaaagga cggttgaaaa atgaaaatcg gactgattgg cagtggacag atgggaaata
     3061 gagtcgaaga agttgcacaa gaaagaggag atacggtcct tcttttacgg acggcaccta
     3121 aagaagcaac tacgcttgct tttacggaac tgccagaact gcttattgat ttttctcatc
     3181 cagataattt agagaaaatc ctttcgtata gcttatcata tcaacttccg ttagtcattg
     3241 gcacgacagg ttatacacaa tcacagttcc aagaaataaa agaacatgcc aaacatgtac
     3301 ctgtgatgta tagtgctaat ttttcgttcg gcatcatggt gatgaatcaa ctaatcaagc
     3361 aagcagctgc aatgttgcaa ggctggcaga tcgaactgat tgaaaaacat catagtcgta
     3421 aaaaagatgc accatctggt actgcaaata tgctgcttca aacaatccaa gaagtgcaaa
     3481 agctgcagcc cgtatacaac tggcaaggga aaaaaagaga agaaaaccaa attggtgtac
     3541 acagtattcg tgcaggttct ttacctggtg aacatgatgt attgtttgct gctactgatg
     3601 aaatatttac gatcaaacat gaatcttttt ctaatcgaat ctttgctgca ggcgcagtag
     3661 aagcagcgga ctggttgaaa gatcagtcac caggttatta caaattagaa gatttattaa
     3721 tggacaaagg ggtgtaaaaa acgtggaagc tcaagaaatc atccgctaca ttcaaacggc
     3781 atctaaaaag acgcctgtga aagtctatct gaatgtaacc gagccaatcg aatttaaaga
     3841 aagtaaagtt tttggtgaag gaagcagctt cgttgttttt ggtgactatg agacgattgc
     3901 tccggtttta gaagctgaat cagaaaaaat cattgaaatc gaaatcgaaa ctgctgcaag
     3961 atacagcgca gttcctttgc tagatattaa aaaaattaat gctcgtatcg aaccaggtgc
     4021 tattatcaga gatcaagtgt caatcggcaa caatgccgtg atcatgatgg gagcgattat
     4081 caatattggt gcagtaatcg gtgaaaacac gatgattgat atgggtgctg tcttaggtgg
     4141 acgagcgaca gtgggcaaaa actgccatat tggtgcagga gctgtcttag caggtgtgat
     4201 cgaaccagca tcggccaaac cggttatcgt agaagacggc gtactggtag gtgcgaatgc
     4261 agtgattgtt gaaggtgtcc atatcggaaa agatgcagtc gtggcagcag gagcagtggt
     4321 tttagaagat gtggcagcag aaactgttgt cggtggaata cctgcacgtg tattaaaaat
     4381 aagtgatgat cagacaaaag aaaatacagc attgattgct gcgttacgcg aattatagag
     4441 gagatggaga aaaatgcgcg aaatagatac ctgtattgtg ggggcaaccg gattagtcgg
     4501 ccaaacgatg ctcaaagtgt tagaagaata tgattttcca gtaggacgat tgaaattatt
     4561 agcttcaaaa cgttcagccg gtaaagtttg tgtattcaag aaaaagaaac acactgtaca
     4621 ggaattgaca gaaacttcat ttgaaggata tgatttagcg ctattttctg caggtgcttc
     4681 tgtatccaaa gagtttgcgc caatcgcacg aagtcaggga ctatttgtca tagataattc
     4741 ctcagcatgg cgggaagatt cggatattcc attaatcgtt ccagaggtca atctggaaga
     4801 ctatacattg aatcggctga ttgcaaatcc aaattgttca accattcaag cggtacttcc
     4861 gcttaaagct ttacaagaag cctttggtat ccagcgagtc aattattcga cctatcaagc
     4921 agtttctggt tccgggcaaa aaggcattga agatcttcat cgtacgcaaa atgacgagga
     4981 accagttttg tatccttata atattagcca aacggtgatt ccggaaatcg atcagccttt
     5041 ggaaaatggg tatacaaaag aagaacagaa aatgataaat gagacccgta agattctgca
     5101 tcagcctgct ttgccagtat cagcaacctg cgtacgtgtg ccaatcgaaa atggacatgg
     5161 agtctctgtg gcagttcaac tgcaaaaaga gtttacgacg gaagaaatta gagagtgtct
     5221 cagccagttt ccagggattc aattagaaga tgatctagca gcacataaat atccgacgag
     5281 cattcttgct agaggaacag atatggttta tgttgggagg atcagaaaag acacttcttt
     5341 agagaacgga ttgttgttct atacgactgc cgataacata cgcaaaggag ccgctgcgaa
     5401 tgccgtacag attgctgcag ctttaatgca gaaaatagcg gaggtggcaa gatgagtaat
     5461 acgctgaaag tagcgaaatt cggcggaaca tcgatgtctc atgaagagca attcaaaaaa
     5521 gtccgtacaa ttgttctttc agatcccaat cgccaagtga tcgttgtttc tgctttaggc
     5581 aaacgtacgt cagatgatga caaagtaaca gacttacttt atttgattta tgcttacctt
     5641 cagcatcatg ttgattggac acccctttgg aaaaggattt gtgatcgttt cattcaagta
     5701 agagatgttc ttgccctgca gttacctatt gaagatgaac tagcacgcat acagaaacaa
     5761 ctggaagacg aaaagataac tgaagattat ttagtaagta gaggtgaata tctgactgct
     5821 caactgatgg ctgagtatct tggttaccag tttattgacg caaaagacct gattttcttt
     5881 gactatgatg ggaaaatcga tcaggaaaaa acagcacagg aaatcacaaa agcatttaaa
     5941 agatatccga aaatggttgt tccagggttt tacggggcga accctgcagg aaaagaaaaa
     6001 ttattgggcc gtgggggaag cgacattacc ggcgcgatac tggcgaagat gttgcaagcg
     6061 gaaagttatg agaattggac ggatgtttca ggaatcatga tggctgatcc gcgaatcatc
     6121 gatcatccta aaatcatcga ggaaatctct tttagagaac tgagagagat ggcttatatg
     6181 ggggctaacg ttcttcatga agcagctgtt ttcccagttc aagaggctga tatccccatc
     6241 caaataaaaa ataccaatga tcctttcgct caagggacaa aaatatcgaa tcacgaaata
     6301 gaaaaggaaa atggactgac aggaatagct ggacgcaagg attttctttc catcacttta
     6361 ttcaaacgtc atatgtccga tgaaatcggg tttatctgga aagcaatgag catttttgct
     6421 aaacatgaga tatcaatcga acatattcct tcagggatcg acaatatcgg agtgatcgtc
     6481 tcagcagaag cgattgccga taagttattt ctcatcacga aagaattaaa agaaacactt
     6541 ggcgtggaag aaatcgtcgt gatcgaagac ttagctttga tctctgtcgt tgggggtccg
     6601 cataaagaat tgattggatt atctgggaaa gtgttatcga tattgaacaa attagaaatt
     6661 cgcacatcga tcttatcaca aggagcacag gagttgaatc tgatcatcgg tgtaccgaat
     6721 aatcaatatg aaacagtagt gaagggtatc tatgaaggga tggtgaatac ggatgctgaa
     6781 agaatatcga atggcactcc atcaaattcc tgaaatcggg tttcatgaat tcaagacaaa
     6841 aaaatattta tatgaacaag taaaaaacag cgaggccgtt atccatgaaa tcggcgaaac
     6901 aggactactc ctttattttg ataatcagca agaagaaacg attgcttttc gaacagatat
     6961 cgatgcactg ccagtcaaag aagaaacgaa tctctctttt gcttctaagc atgaaggtta
     7021 tatgcatgca tgtggtcatg acggacatat ggcgatgctt ttagggttga ctgactttat
     7081 aactaaacac attgaggatc ttatttataa tgtagtactg atctatcagc catcagaaga
     7141 aatttccggt ggcgcacaaa gcgttattca gtcaggattg ttggataaat atgaagtaca
     7201 agcaatattt ggctttcatc tatggcctgg actagctaag ggagaagttt tctctcgacc
     7261 aggacctttg atggcacaaa gcagtgaaac agacattatc attaaaggcc agtctgcaca
     7321 tattgcttct agtgaaaaag gaatcgatag tctggaggca gctgtacgat ttttgaagaa
     7381 tgtttatgat tttgaccggc aattaccggc agagtggaat catctaatga aatttggcca
     7441 aatccaaggt ggaacgatcc gaaatgtttt agctggagaa gttattgtat ccggaagtat
     7501 ccgttcttac agttcagata tgcaaaatta tttgaagagg ggattagacc atttagcaga
     7561 acagtttcac tctacagcta ctatttcttt tcgctataat gacggctatc cagctgtaat
     7621 caaccataga ggcttatatg cagcactgtt agattcaaac aaactacatg aattagatgc
     7681 tccagttctt caagccgaag attttggcat atatacgcaa caatatccat gtgtattttt
     7741 ctttttaggt gtcggtgata cccctgcttt acatgatccg acgttcgatt ttgatatgga
     7801 tgttttagaa aatggtgtag aatggtacaa aacgattctt tttacagaaa atcttcttgt
     7861 aaaaatcgat agcagttcaa aagaggagcg agtaaataat gatgaattta tccaatcgat
     7921 ttagtcctag gctttctaga atagaagtat caaaaatacg acaattcgat cagcaaatat
     7981 cgtcgatacc agatgtgatc aaactcaccc tgggagaacc ggattttcca actcctgaac
     8041 atgtcaagca agcaggaata gcagcgattg aagaagactt ttctcactat accggaatga
     8101 gaggattaga ggaactgcgg gaagcggctt gtatatttca gcaacagcgt tatggattaa
     8161 cttatgatcc gcaaacggaa gttttgacga cagttggtgc aacggaagcg attgcctctg
     8221 cgttgttatc tgttttggaa gaaggagaca aggttcttat tccagcgcct gcttattcag
     8281 gatatcagcc attagtggaa ctagctggtg ctgaattgat cccgatagat acttctgaca
     8341 cagggtttgt ctgtcagccg gaacaatttg aacgagcatt tgaacaatat ggatcagctg
     8401 tcaaagcagt cattttaaac tatcctaaca atcctactgg gacgacattg tctgcaaaac
     8461 aattgaaagc aatcgctgag gttttaaaaa aatatccggt atttgttata agtgatgaag
     8521 tatatgctga attgacttat agtggaacgc atatgtccat agctacttat cttccagaac
     8581 aaacaatcgt gataagcggg ttatccaagt cacatgcgat gactggttgg cgagtgggct
     8641 ttatttttgc tcaaaaagcc cttattgacg aactaattaa agtccatcaa tatctggtga
     8701 ctgccgctac aacgatgtcg caaaaagctg cggctgaagc gctaataaat ggggtgaaag
     8761 atagcgagaa tatgaaagag caatatcaaa cacgaagaga ctatctggtc cagcaattgg
     8821 cgcctttagg atttgaagtg acccagccaa acggtgcatt ttacttgttt tgcaaactcc
     8881 cagctgccat tcaaactgac tcttggcagt tttgtgtaga attagcggaa caggcaaaag
     8941 ttgcgtgtat cccaggaatt gcatttggcc cagaaggaga aggttatatc cggatttctt
     9001 atgctagcag catggaaaaa ttacatgagg cctgcaaacg gatcaaagac tttttaacag
     9061 aacagtgatt catagatttt atcaaaaaaa caatcgttaa ttttgcggta tattctgcaa
     9121 ggagacgatt gttttttaaa tttagttcaa atgaacgcgt ttcttttgat tcataactaa
     9181 tgtaatgtta agtttaaatt gtttagaatg attttaaaag aatattcaag gaggtttcat
     9241 caatggaaaa aatcaaagtt gtcattgtag gtgcatctca tggagggcat caatcgatct
     9301 tggaactatt gacacgatac ggtgaaaatg tagatatcac gctatttgaa gcaggagact
     9361 atatttcctt catgtcatgt ggtatggagc tgtatttaga agacaaggta acagatgtaa
     9421 atgatgtacg taatttcaga ccagaaaact ttccgcaatc caatgttcat attttaaata
     9481 atcacgaagt aaaaacgatc gatgccgata aaaaaacagt aacggttact agcgcatcag
     9541 ataacgaaac aaaagaatat ccttatgaca aattgatttt gagttctggt gtcaaaccaa
     9601 acaatctgcc agtaccagga gcagatctag aaaatgtcta cttgatgcgt ggatacaatt
     9661 gggcaaccaa aatcaaagaa aaattaaatg atccggctat taaaaaaatc gctgtcgtag
     9721 gtgcgggtta cattgggatc gaagcagcag aagcaagccg aaaagctggg aaagaagtcg
     9781 tattgcttga tgtgatcgat agacctttag gcacttattt agatgctgag atgacagata
     9841 ttttagaaca acacctaaat gaaaacggta ttgaagtttt gacaaatgca aaaatcgaag
     9901 cttttactgg taacggaaaa gttgaggcaa tcaaaacaag tgaaaaagaa atcccagcag
     9961 atctagtcat tcaagctgcc ggtgtgaagg caaatacaga atggttaaaa gggatcgtcg
    10021 acttagatga acgtggctgg atccaaacaa atgaatactt gcaaacgaat cttccagatg
    10081 tgtatgcagt aggagatgct acacttgctt attctatccc agcacgcaaa aaaatgccga
    10141 tcgctttagc gactgttgca cgtagagaag cacgttatgt agtaaaacat ctttttgaag
    10201 aagtaccaaa caaaccgttc ggcggagtcg taggttcctc tgcattaagt gtctttgact
    10261 atcattttgc agcaagcgga ttgaatagct ttacagcagc aaaagcagga gtagatgtac
    10321 gagtgtctta ttatgaagat acgttacgac caaaatatgt accagaagaa caaggaaatc
    10381 caaaagtgtc catccaatta ttctcggatc caatgaccca tcaattgcta ggaggagcag
    10441 tattatctac ctatgatgtt acaggtcaag gaaatgtatt ggcactagcg atccaaaaca
    10501 aactgacttt agaagaacta gcagaagctg acttcttctt ccagccaggc tttgaccgac
    10561 aatggagcat gttgaatcta gccgctcaac aagcgttagg agaagaacca tttgtagaat
    10621 aagaaaacga aatatttgaa aagaggttgt gaaaaagctg ccttgcatca agtaataaga
    10681 acagccaagt aaaaacagct tcttatgttt ttaacttggc tggacttatt accgcagatg
    10741 caagcttttg aacaccgttt attttcaaaa aagggactgt gacacgattt gtgtaccgac
    10801 ccccaaaagt tagaccaaaa aatctaactt ttggaggtcg gtatttttta tggttaaata
    10861 tacttttgaa cttaaattaa agatcgttca tgattatttg gatggcaaag gcgattggac
    10921 cgtaaaaatc cagatcaaga gattgaagcg gaaatgctta aaattcggga gaagcacaag
    10981 gattatggct gtttacggat gaccaatgaa ctaagaaacc gcggtttctc tatcaataaa
    11041 aagaaagttc agcgcttgat caagaagcta ggaataaaag taacggcata cacgcgtaaa
    11101 tcacgtcgct acaattctta tcgaggaaaa gt
//
LOCUS       DAZKOU010000094        10711 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000094 DAZKOU010000000
VERSION     DAZKOU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.095"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..957)
                     /locus_tag="ACG827_002386"
                     /pseudo
     CDS             complement(1..957)
                     /locus_tag="ACG827_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            1448..2386
                     /locus_tag="ACG827_002387"
     CDS             1448..2386
                     /locus_tag="ACG827_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0484497.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2516..3163
                     /locus_tag="ACG827_002388"
     CDS             2516..3163
                     /locus_tag="ACG827_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0484498.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(3856..4794)
                     /locus_tag="ACG827_002389"
                     /pseudo
     CDS             complement(3856..4794)
                     /locus_tag="ACG827_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4794..5411)
                     /locus_tag="ACG827_002390"
     CDS             complement(4794..5411)
                     /locus_tag="ACG827_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0484499.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(5425..5595)
                     /locus_tag="ACG827_002391"
     CDS             complement(5425..5595)
                     /locus_tag="ACG827_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484500.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(5943..7433)
                     /locus_tag="ACG827_002392"
     CDS             complement(5943..7433)
                     /locus_tag="ACG827_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0484501.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(7476..7775)
                     /locus_tag="ACG827_002393"
     CDS             complement(7476..7775)
                     /locus_tag="ACG827_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484502.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8141..8425)
                     /locus_tag="ACG827_002394"
     CDS             complement(8141..8425)
                     /locus_tag="ACG827_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0484503.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(8944..9429)
                     /gene="vanZ-A"
                     /locus_tag="ACG827_002395"
     CDS             complement(8944..9429)
                     /gene="vanZ-A"
                     /locus_tag="ACG827_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0484504.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(9582..10493)
                     /locus_tag="ACG827_002396"
     CDS             complement(9582..10493)
                     /locus_tag="ACG827_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0484505.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3308 a         1864 c         1658 g         3881 t
ORIGIN      
        1 acttttttct gcttcttttt ctttctgttc ctggtaatca gaaaaattta gtttttctat
       61 atcgttaggt acagcttcca gtaaatgaac aatgaatttt ttgatattcg taataaagtt
      121 ctcttgattg ccttctcttt taccgatttt ttttaaatac gtttcccatt tagccgtcat
      181 ttcagcactc gttaaaaggt gctgactttc aactgcctgg cacaataatt ttcctttttc
      241 agttacaaca agcttattct taatcacttg gatatattct ttttgtttta acgcttcaat
      301 aatacttgct cttgtcgctt ctgttccaat cccctcaact tcttgtaaaa tcttgattgc
      361 ttcttcatca tcaaccgttt tattcgccgt tttcatagca gttaataatg taccctctgt
      421 aaaagctttt ggcggttgcg tttctttttc aacactctta acaccaactt cggcacgttc
      481 gccaatgata acgagtggta acgtttgaac gtcctctttt tcttctttgg tttgttgttt
      541 gaaaagaatt ttccaccctt cttgctttgg tgtctttccg atcgattgaa acagcaaatc
      601 agctactttc gtttgtatct tggtttcttc atacaagtaa tcaggtaaaa acatggcaac
      661 ggtcgtttta acgactaaag cataaatttt tcgttgtaaa tcgtccattt tcgctaatgc
      721 agattcggta ggtacttgtt ttgttgggat aatggcgtgg tgttcctgta ccttactacc
      781 gtccacataa cgctttctag gctctgtttg aaccatttca agatcaagtc ctaaaaaacc
      841 gctatatttg ccaaaattcg cttttaaata agcaaattcg ttctctgtaa taaatggtgt
      901 atctgttctt ggataactca ataattttgc ttcatacagt ccttgcatag cttttaaacg
      961 taaaattttg cgttctaaga cgttttaaat atatctgcgt atatttttat gtgttttatc
     1021 ttttcgtcgg caaatgcgtt aaaatttgcg aaataaaata gctcaagggc gaacgcagtg
     1081 agtgcattta cccttgatct atttttttga gtaaattagc ttttgaaacc gacggaaagg
     1141 aaacacaaaa cttttcagag gcaaggggtt tttaaggggc gcttttagcc ccttgaattt
     1201 atttgcttta agatatattt agttttaaaa tagttttaac gaaaaaaaga cataaaaaaa
     1261 gacgagcaac gctaccaacg ttctcgcccg gaataacaaa ccacacttgt tattcattcg
     1321 atgattagta tatcaatttt tgattttgaa tacaaaacaa aatggacgaa tggatacagg
     1381 cgaaatttat tttgcgattc cactcgtcct tttttcgttc caaaaattat tttaggagcg
     1441 ataaaaaatg aagaaaattg atgaaaaatt agctgcagaa atctcactaa aaaaaccggc
     1501 cgtctctaat agccggttaa gtaatagccg gttaagtggt caaactttgg gaaaatctca
     1561 acccgcatta agttttgatg ccatgacaat cgttggaaat ttgaacaaaa ctaatgctaa
     1621 aaagctatct gactttatga gtgtagagcc acaaatacga ctttgggata tacttcaaac
     1681 aaagtttaaa gctaaggcac ttcaagaaaa agtttatatc gaatatgaca aagtaaaagc
     1741 agatacttgg gatagacgta atatgcgtgt tgaatttaat cccaataaac tcacacatga
     1801 agaaatgatt tggttaaaac aaaatattat cgactacatg gaagatgacg gttttacaag
     1861 attagactta gcttttgatt ttgaagatga tttgagcgat tactatgcaa tgactgataa
     1921 agcagttaag aaaactgttt tttatggtcg taatggcaag ccagaaacaa aatattttgg
     1981 tgtccgtgat agtgatagat ttattagaat ttataataaa aaacaagaac gtaaagataa
     2041 cgcagatgtt gaagttgtgt ctgaacattt atggcgtgta gaagttgaat taaaaagaga
     2101 tatggttgat tactggaatg attgttttaa tgatttacac atcttgaaac ctgcgtgggc
     2161 tactttagaa aaaattaatg agcaagctat ggtttatact ttgttgcatg aagaaagtat
     2221 gtggggaaag ctaagtaaga atactaagac taaatttaaa aaattgatta gagaaatatc
     2281 tccaattgat ttaacggaat taatgaaatc gactttaaaa gcgaacgaaa aacaattgca
     2341 aaagcagatt gatttttggc aacgtgaatt taggttttgg aagtaaaata agttttattt
     2401 gataaaaatt gctaattcag tataattaat atttacgagg tgacataacg tatgaaaaaa
     2461 tcagaggatt attcctccta aatataaaaa tttaaaattt aggaggaagt tatatatgac
     2521 ttttaatatt attgaattag aaaattggga tagaaaagaa tattttgaac actattttaa
     2581 tcagcaaact acttatagca ttactaaaga aattgatatt actttgttta aagatatgat
     2641 aaaaaagaaa ggatatgaaa tttatccctc tttaatttat gcaattatgg aagttgtaaa
     2701 taaaaataaa gtgtttagaa caggaattaa tagtgagaat aaattaggtt attgggataa
     2761 gttaaatcct ttgtatacag tttttaataa gcaaactgaa aaatttacta acatttggac
     2821 tgaatctgat aaaaacttca tttcttttta taataattat aaaaatgact tgcttgaata
     2881 taaagataaa gaagaaatgt ttcctaaaaa accgatacct gaaaacacca taccgatttc
     2941 aatgattcct tggattgatt ttagttcatt taatttaaat attggtaaca atagcagctt
     3001 tttattgcct attattacga taggtaaatt ttatagtgag aataataaaa tttatatacc
     3061 agttgctctg caacttcatc attctgtatg tgatggttac catgcttcac tatttatgaa
     3121 tgaatttcaa gatataattc atagggtaga tgattggatt tagtttttag attttgaaag
     3181 tgaatttaat tttatacacg taagtgatca taaaatttat gaacgtataa caaccacatt
     3241 ttttggttgc ttgtggtttt gattttgaat ttggttttga acttatggac tgatttattc
     3301 agtccatttt ttgtgcttgc acaaaaacta gcctcgcaga gcacacgcat taatgactta
     3361 tgaaacgtag taaataagtc tagtgtgtta tactttactt ggaagatgca ccgaataaaa
     3421 aatattgaag aacaactagc aaaagatttt aaagagttat tttattttaa gtctttataa
     3481 catgagtgaa gcgaattttt aaatttcgat agaaattttt acatcaaaaa gccccctgtc
     3541 aaaattgacg aagggggttt tttggcgcac gcttttcgtt agaaatatac aagattgaaa
     3601 atcgtgtata agtgcgccct ttgttttgaa cttagcacgt tacatcaatt ttttaaaatg
     3661 atgtataagt gcgccctttt aaattttgag tgattatatt ttttgagtta gaaaaaggga
     3721 ttgggaaaat ttcccaaaat aatttaaaaa ataagcaaaa attttcgata gagaatgtgc
     3781 tattttttgt caaaggtgta taccttgact gtgcttgctg ttacattaag tttattttta
     3841 agttattaaa aaagaaatag cttttaaagt ttggctcgct gtcgctttat aaagctgatt
     3901 gacttttgat tgcaaactac ttaaagaaaa caaactcgga ctattcgttt tcttctcttt
     3961 ggtttgaaca tcagcaatta tcccctcttg attgcctatt ttagcttgtt tagaagaaac
     4021 aaaagctaaa agctcctctt gggttttaaa acgctgtgtg gggcttagaa cgcccttaaa
     4081 cgacccttgg tttactttta tactagcttc cacctcgaaa aaaggttctt ttttaaaatt
     4141 ctctatggct tcctggcgct gaaaaataag gtataaggtg ggcgtttgaa cacgtcctag
     4201 tgaaaatgta ccttgtacgc ccttctgttg taaatttaac gtatacaaag ggcttgcgtt
     4261 catgccgatc aaccaatcgg caatttggcg tgtttgcgct tcttgataaa agggatagta
     4321 attcattcca ggttgcaaat tttgaaaacc gcttcggatt acatcttttt ctaagctatt
     4381 gatccatagt cttttaaatg ttttatcttt tgaaaaggca tttgctttat ggataatcga
     4441 ccaggcgata ttttcacctt ctctgtcgct atctgttgca acaataattg tatttgcctt
     4501 tttgagaagt tctgcaacaa ttttaaactg ctttccctta tcttttgcaa cttcaaaatc
     4561 gtatcgatca ggaaaaatcg gcaaagattc aagtttccaa ttttgccact tttcgtcata
     4621 atgacctggt tctgctaatt ccactaaatg cccaaaacca aaggtgataa acgtttcatc
     4681 tgtaaatagt gggtctttga tctcaaaata accgtctttt ttggtgcttt gttttaaagc
     4741 acttgcgtag gctaatgcct ggcttggttt ttcagctaaa ataaccgtac tcattaacta
     4801 tccctctttt cattgttttt tctttgatcg actgtcacgt tatatcttgc tcgatacctt
     4861 ctaaacgttc ggcgattgat tccagtttgt tcttcaactt ctttatcgga taaaccattc
     4921 aaaaacaaat cgaaagcatg ctttaaacgt ggatcatttt ctttaaattt atgctgacga
     4981 cctttgaatt tgcctttttt cttagcaatt tcgattcctt gtgcctgacg ttccttaatt
     5041 ttttttcgtt ctgattctgc ttgatacttg tacaattcaa tgacaaggct attaatcaaa
     5101 cgccttaaat tttcatcttc aataccattc attgagggta aatttaagac ttccagggtt
     5161 gcccccttaa tttgaatttg attcatcaat tctgttaatt ctttattatt tcgtcctaat
     5221 cgatctaatt cagtaacaat aacaatatcc ccttcacgaa tatagttaag catagcttgt
     5281 aattgtgggc gttcgaccga ttgaccgctt aatttgtctg aaaagacctt agaaacgccc
     5341 tgtaacgctt gtaattgccg atctaagttc tgttctttgc tactgacacg tgcataacca
     5401 attttagcca ttttcaacca acctctaaaa ttctctcggt tgcaataacc aatcagcaat
     5461 atctactttt tcaatttcaa attgcttatc agaaattgtc ttttcgtaag cgataaaatc
     5521 ttgcgcatat tgttgctcat taaaaatagc caccacttcg tcattttcta aaactcgata
     5581 aataaatttt ttcattttac tcctcctatt atgcccaact taaatgacct attcaccaag
     5641 tcaattatac tgctaaaatc atattaggac aaataggtat actctaatga cctataaaag
     5701 atagaaaatt aaaaaatcaa gtgttcgctt cgctctcact gcccctcgac gttttagtag
     5761 cctttccctc acttcgttca gtccaagcca actaaaagtt ttcgggctac tctctccttc
     5821 tccccctaat aattaattaa aatcttactc tgtatatttc tgctaatcat tcgctaaaca
     5881 gcaaagaaaa aacaaacacg tatcatagat ataaatgtaa tggcatagtg cgggttttat
     5941 tttcagcctg tatcatagct aaacaaatcg agttgtgtgt ccgttttagg gcgttctgct
     6001 agcttgttta aagtctcttg aatgaatgta tgctctaagt caaaagaatt tgtcagcgcc
     6061 tttatatagc tttctttttc ttcttttttt actttaatga tcgatagcaa caatgattta
     6121 acactagcaa gttgaatgcc accatttctt cctggtttaa tcttaaagaa aatttcctga
     6181 ttcgccttca gtaccttcag caatttatct aatgtccgtt caggaatgcc tagcacttct
     6241 ctaatctctt ttttggtcgt cactaaataa ggcttgtata catcgctttt ttcgctaata
     6301 taagccatta aatcttcttt ccattctgac aaatgaacac gttgacgttc gcttcttttt
     6361 ttcttgaatt taaaccaccc ttgacggaca aataaatctt tactggttaa atcacttgat
     6421 acccaagctt tgcaaagaat ggtaatgtat tccctattag ccccttgata gttttctgaa
     6481 taggcacttc taacaatttt gattacttct ttttcttcta agggttgatc taatcgatta
     6541 ttaaactcaa acatattata ttcgcacgtt tcgattgaat agcctgaact aaagtaggct
     6601 aaagagaggg taaacatgac gttattacgc cctattaaac ccttttctcc tgaaaatttc
     6661 gtttcgtgca ataagagatt aaaccagggt tcatctactt gttttttgcc ttctgtaccg
     6721 cttaaaaccg ttagacttga acgagtaaag cccttattat ctgtttgttt gaaagaccaa
     6781 tcttgccatt ctttgaaaga ataacggtaa ttaggatcaa aaaattctac attgtccgtt
     6841 cttggtatgc gagcaatacc aaaatgatta cacgttagat caactggcaa agactttcca
     6901 aaatattctc ggatattttg cgaaattatt ttggctgctt tgacagattt aaattctgat
     6961 tttgaagtca catagactgg cgtttctaaa acaaaatatg cttgataacc tttatcagat
     7021 ttgataatca tagtaggcat aaaacctaaa tcaatagcgg ttgttaaaat atcgcttgct
     7081 gaaatagttt cttttgccgt gtgaatatca aaatcaataa agaaggtatt gatttgtctt
     7141 aaattgtttt cagaatgtcc tttcgtgtat gaacggtttt cgtctgcata cgttccataa
     7201 cgataaacgt tgggtgtcca atgtgtaaat gtatcttgat tttcttgaat cgcttcctcg
     7261 gaagtcagaa caacaccacg accgccaatc atgcttgatt ttgagcgata cgcaaaaata
     7321 gcccctttgc ttttacctgg cttggtagtg attgagcgaa ttttactatt tttaaatttg
     7381 tactttaaca agccgtcatg aagcacagtt tctacaacaa aagggatatt cattcagctg
     7441 ttctcctttc ctataaatcc tataaaatag gttgtttaat taacttggtt tgctttttca
     7501 ttcaactgtt tcaatattgc atgttttgaa aaagattttt ttcctttata agtcaatttt
     7561 tttccactaa tcgaataaat tattttgtta ttttctatta acttatatat ataatcttcc
     7621 ccctccgaag aaaaatactt atctgatttt gtttctaagt agatatttct cttttctaac
     7681 tctttcttaa acgtttctag tgtatagata tttgctaatt ttcttatctc caataaacta
     7741 ttttttatat aagttttaca ttcatcatga ttcatacaaa ctccaccttc tataaatgaa
     7801 tacaaaaaaa gcaatcaaac gatttccgat tgattgctta acaattctta aattcagtag
     7861 cttagatact tgaaaactct ctgatttccc tatataatga tagtacggtt atataccgtc
     7921 ttcaaacaaa gttaattaaa taacttctta cgagggaaga gttcatctga ctaactgata
     7981 agcgttggtt tggcaatctt atcgggctat gcatttataa aatgtcgtca aacattttat
     8041 aaatgtgtca tggctctttt ttcgtttcta ttcagttcgt tgtttcgtta tatctagtat
     8101 accgctttta aaaaaaataa gcaacgattt cgtgcattat tcacacgaag tcattgcttt
     8161 tttcttcttc catttctaaa tccaatgtta cttgttctga ttctgtttct ggttctggtt
     8221 ctgttggctc atttgggatt aaatccacta ctagcgttga gttagttaac tttgcaattt
     8281 gttctagtgt ttttatggtt ggatctgatt ttcctgattc tattcgtgaa taatttgatc
     8341 tactcatttc taattcttgg gagaattttt cttttgattt ggtacctctc atctttttta
     8401 agctttctct aaatgctagt tccaagtttc tcccccctct ttttgtgtaa taaaattcac
     8461 gctttattta aattatagct tttaatatct taataaacaa gtgattaatc acttgtgatt
     8521 tgtgattatt ccaaaagtag gtaatttcac caaccttttt caaaagtttt tatcgacgat
     8581 tattggactg gatcgtgttt cctaaagcga ttctaagcgc atgtttaatt ttaaagtggg
     8641 taattctctt taaaacgtcc tacaaagatt caaaaaaagc atggctgcta gtgggggtac
     8701 cgtcaggaaa atgcggattt acaacgttaa ggaaatttat taaagagacc aattcctcag
     8761 cactaaaatt gataattggg ctttctttgt tactatagtg ttaggtaagc tttttacttt
     8821 atcaccggtt ttcccctcac ttcacaccta cgggcgagtt tccccacatc ccgttttcca
     8881 tctaatccaa ttcattcaat atctttatgt gtatgaaaga tcagattgct tgatttagac
     8941 atcttacacg taatttattc ttaaatgggt acggtaaacg agcaataata cgagcaaaag
     9001 tatacctaca aaaataataa ctctgtctaa ttttttttga ttcatatgct tattgcttaa
     9061 accatataat ttcagtccaa gaaagcctcc aacagtattt gtaattacat ctgttatgtc
     9121 tgtcgctcca atagcgaaga taaattgaat tatttcaaaa gtaagactta aaaccagtac
     9181 aaaagcaaac ttaggtaaaa atccgatttc tttaaaattg acattcaaaa gcaagccaaa
     9241 tggaataaag attataacat tatctatcat ctctctgaaa ttcccagtag cagtaaatgg
     9301 agtcaagtta agacttcttt gatgataatt aaatactgat aaaatattgt attgtaattt
     9361 gaataacact aaccagatta gtgtcactaa atataaagct agcaatcctc tagataatat
     9421 ttttcccaat ataatttcct ctaattcttt tgtttaagtt gtatcattag tatagcaatg
     9481 tcctagttaa cgtgggtttt aaattaaaaa gctaacgtta ccccaaaagt gctagtacaa
     9541 taatgctgtt cattaatttc gttggtttca ttccgccatc cttacctcct tgaattagta
     9601 tgtgttgatc cgggaaacac tgtcacaatt acaccgtcta tattgtttcc tgatatctca
     9661 taacgaagat tagtcggcac atgaatggtg gtatttttag taacaggata ataaaagatc
     9721 tcatattttt ccccatttac actaacagaa atggtttttt cttcttttag gtaatccata
     9781 tattcctcga gaacgaaatt cttttctttc ataatcgcac tatgtggtaa accaacatag
     9841 cgaatatgcc atggttcata ttgaattcct gttaactctg ttttgtcctc tggataacgt
     9901 aaaatgaacc cgtatttcca agcattttct tctatccact ttccttcagg ggctcgttcc
     9961 attttcgtca agcttgatcc tacatctagt gataaacctg aattatgctc actataacct
    10021 gctggtaagg catactcagc ccccatttct tggtaaagca cactttgctc atcaaagtct
    10081 cgatagccac tattaataat aaaatgacta acgccaccct ttacagcatc attgaccatc
    10141 tctgaaaatt tttgtgctat ttcttttgac atataaatat tactatcaag caacccgtat
    10201 ccatttatta attcgtcatg tttagataaa ttcacgatat ctgacttcac actttcttgg
    10261 cgaacaggat atttactatt gattaatagc agatttcctt gataaacctg ttcttctgta
    10321 attgttttct cttgggtatt ttcagaagtc ccactatttt ctaaatgttc tttgggattt
    10381 tgatcataat tttgaaattc gactttttcc tgagaaaaca gtgcttcatt aacgtagtca
    10441 taacctaagt atattaagaa taacaataac aataaaaaaa acaacttctt cattttcagt
    10501 ctcctcctta actatatagt tggaagtatg aaatatttct agaatcttcc ccttaaacgg
    10561 cacaatctcg tttgagattg tgccgttttg ccccgtaaat tacagtcaat tatttcgccc
    10621 tactggattt actattatca ccaatgtaga tgaattttca taagtgatac ccatgctgaa
    10681 tttaacgagg ctgaactgcc tgttgacaat g
//
LOCUS       DAZKOU010000095        10661 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000095 DAZKOU010000000
VERSION     DAZKOU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.096"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACG827_002397"
     CDS             34..657
                     /locus_tag="ACG827_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484506.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACG827_002398"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACG827_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0484507.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACG827_002399"
     CDS             2153..3034
                     /locus_tag="ACG827_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0484508.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACG827_002400"
     CDS             3037..3807
                     /locus_tag="ACG827_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484509.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACG827_002401"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACG827_002401"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0484510.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACG827_002402"
     CDS             5118..6281
                     /locus_tag="ACG827_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0484511.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACG827_002403"
     CDS             6398..6808
                     /locus_tag="ACG827_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0484512.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACG827_002404"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACG827_002404"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0484513.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACG827_002405"
     CDS             9381..9665
                     /locus_tag="ACG827_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484514.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="ACG827_002406"
     CDS             9825..10061
                     /locus_tag="ACG827_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0484515.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="ACG827_002407"
     CDS             10058..10456
                     /locus_tag="ACG827_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0484516.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3725 a         1773 c         2253 g         2910 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
    10621 gaaatagtgg tttagaacta gtgggctgat tttttatata t
//
LOCUS       DAZKOU010000096        10517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000096 DAZKOU010000000
VERSION     DAZKOU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.097"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACG827_002408"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACG827_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACG827_002409"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACG827_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACG827_002410"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACG827_002410"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0484517.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACG827_002411"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACG827_002411"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0484518.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACG827_002412"
     CDS             5704..6489
                     /locus_tag="ACG827_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0484519.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACG827_002413"
     CDS             6502..7353
                     /locus_tag="ACG827_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0484520.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACG827_002414"
     CDS             complement(7477..7929)
                     /locus_tag="ACG827_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484521.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="ACG827_002415"
     CDS             complement(8394..8762)
                     /locus_tag="ACG827_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0484522.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="ACG827_002416"
     CDS             8912..9394
                     /locus_tag="ACG827_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0484523.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="ACG827_002417"
     CDS             9431..10441
                     /locus_tag="ACG827_002417"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0484524.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         3576 a         1748 c         2192 g         3001 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaa
//
LOCUS       DAZKOU010000097        10372 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000097 DAZKOU010000000
VERSION     DAZKOU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.098"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG827_002418"
     CDS             6..305
                     /locus_tag="ACG827_002418"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0484525.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG827_002419"
     CDS             378..1739
                     /locus_tag="ACG827_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0484526.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG827_002420"
     CDS             1761..2021
                     /locus_tag="ACG827_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484527.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG827_002421"
     CDS             2035..2787
                     /locus_tag="ACG827_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0484528.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG827_002422"
     CDS             complement(2881..3486)
                     /locus_tag="ACG827_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0484529.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG827_002423"
     CDS             complement(3539..4513)
                     /locus_tag="ACG827_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0484530.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG827_002424"
     CDS             complement(4891..5610)
                     /locus_tag="ACG827_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0484531.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG827_002425"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG827_002425"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0484532.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG827_002426"
     CDS             7161..8558
                     /locus_tag="ACG827_002426"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0484533.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG827_002427"
     CDS             8639..9079
                     /locus_tag="ACG827_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0484534.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG827_002428"
     CDS             9227..9553
                     /locus_tag="ACG827_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0484535.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG827_002429"
     CDS             9554..10303
                     /locus_tag="ACG827_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0484536.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3288 a         1830 c         2150 g         3104 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tc
//
LOCUS       DAZKOU010000098        10146 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000098 DAZKOU010000000
VERSION     DAZKOU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.099"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..493
                     /locus_tag="ACG827_002430"
     CDS             20..493
                     /locus_tag="ACG827_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0484537.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            502..729
                     /locus_tag="ACG827_002431"
     CDS             502..729
                     /locus_tag="ACG827_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484538.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1365..1736
                     /locus_tag="ACG827_002432"
     CDS             1365..1736
                     /locus_tag="ACG827_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484539.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1992..2234)
                     /locus_tag="ACG827_002433"
     CDS             complement(1992..2234)
                     /locus_tag="ACG827_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0484540.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2266..3168)
                     /locus_tag="ACG827_002434"
     CDS             complement(2266..3168)
                     /locus_tag="ACG827_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0484541.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3181..3369)
                     /locus_tag="ACG827_002435"
     CDS             complement(3181..3369)
                     /locus_tag="ACG827_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0484542.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3383..3946)
                     /gene="amaP"
                     /locus_tag="ACG827_002436"
     CDS             complement(3383..3946)
                     /gene="amaP"
                     /locus_tag="ACG827_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0484543.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3984..4877)
                     /locus_tag="ACG827_002437"
     CDS             complement(3984..4877)
                     /locus_tag="ACG827_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0484544.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4955..5878)
                     /locus_tag="ACG827_002438"
     CDS             complement(4955..5878)
                     /locus_tag="ACG827_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0484545.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5927..6277)
                     /locus_tag="ACG827_002439"
     CDS             complement(5927..6277)
                     /locus_tag="ACG827_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0484546.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6310..7212)
                     /locus_tag="ACG827_002440"
     CDS             complement(6310..7212)
                     /locus_tag="ACG827_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0484547.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7205..8062)
                     /locus_tag="ACG827_002441"
     CDS             complement(7205..8062)
                     /locus_tag="ACG827_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0484548.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8396..9205
                     /locus_tag="ACG827_002442"
     CDS             8396..9205
                     /locus_tag="ACG827_002442"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0484549.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9245..9742
                     /locus_tag="ACG827_002443"
     CDS             9245..9742
                     /locus_tag="ACG827_002443"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0484550.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         2930 a         1920 c         1748 g         3548 t
ORIGIN      
        1 agataaaaga gggataaaaa tgaaatttaa aacattgcta ttaaaatttg tcgttttagg
       61 tataagtgcc gtatttattt tgttcggcgt acttttttat actcagatcg ctacaagcga
      121 aaaaggattc aattttgatt ggcagaccac attatttgtt tctgtcattt actttgccct
      181 tctcctaggg atcgttattg cttatttctt attgaaacta ttgaaaaaaa tcgaagataa
      241 ccaagctttt tccactcaaa gcttaaacat actaaaaaaa atcgaacgaa ccattttgtt
      301 tattggtttc gccttttttg gcttacttcc taaatttatg aaagtagcgg atttagacga
      361 tgctccaggt ttgatgttgg taggaatcgc tgtcgtattc attccttttg cagtatacac
      421 attaacaagc gtgctggaaa aactgcttga acatgcgatt ttacttaaga cagatcatga
      481 tttgacagtg tgaggtgcga catggcaatt atcgttaatt tagatgtagt attagctcaa
      541 aaaaaaatga gtgtgactga attgagtgag aaagtgggca ttacgatggc aaacatctct
      601 attctaaaaa atggaaaagc aaaagccatc cgtttttcca ctctcgaaaa aatctgccaa
      661 gttttggagt gtcaaccagg tgatttgttg acctataaaa atgactcgaa tactatacca
      721 gaagaataaa ataaagtaga acaaacagta aataaaaaat aatctgaatg atagatgtgt
      781 gagactttta gaagttattt acgtctatta ttcagatttt ttatttcgat tgaataccgt
      841 tttatctata aataagatat gtactgactc atacaaaggg ggccttttct tctccatgta
      901 ctatcacttt tcctgataca actggtttcc tgttacgttg tagttggatc tgcttttagt
      961 ataatacttt attcattcca ccagactgat agcagtttga gcagtctaaa taatcatgta
     1021 gtattacagg ccattctgtt tcataccttg aagtgcttaa aacttcagct aatggcccat
     1081 tgtgttaact cttactccat tcttttttag taagctccca tatatactgt aaagccttct
     1141 atatattatg tcatgttgct ttttaacatt tactatgtag ttttgataaa atgatttgaa
     1201 taacagtgat ggacaattct tttttatttt taacattatt ttgataataa tgattattta
     1261 tttaaaaata cattagataa catttgttac gacacgaaaa agcttcacaa acaaatatcg
     1321 ttattttaaa atgagggcga actcacacaa ttaggaggaa tagtatgcca tttattgttt
     1381 acttttttct ttcattactc gtgatctata ttccattacc aacatttttc ctgctcgtca
     1441 atcgtctagc agagcaacat gatacgattg ctgctcttac gaaactttgt ttacttctat
     1501 ttgtttgtct agattacttt acgagctatt attggctaaa acagacaaga gtaaaaaaga
     1561 gatattattt cttccttgtc tttttcaatc tctttgagtt tctgatccat tgctttttac
     1621 ttcatcagta tcatacgatc catttgtgga tcatctgggt tttcaaagtg gtcgtattat
     1681 tttgtatgtt cacctttcct atctcaagaa aatttaggga ggcattgttt tattaaagaa
     1741 gcttctctgt atcaataaag acacatttaa ggaaaaactt ttaaaaatct aattgggaat
     1801 ttgcccgcat aaataagagg ctgtgacaaa agtcttgtca cagcctctta ttccgtataa
     1861 acagtgttca aaagcttgca tcttcgataa tatgcccaac caaatgaaaa atatgagaag
     1921 ctatttttgt ttggctgttc ttattacttg atgaagaaca gctttttcac gacctctttt
     1981 taaaagtctt tttacagttt tcgagcaaag aatgatacga ttagtactaa aatgatggca
     2041 ccaatcaatg atggcacgat tgccattcct gcaatacttg gtccccaaga acctaataat
     2101 gcttgaccaa gtgctgatcc aactaaacct gctacgacat ttgcaataat ccccatcgaa
     2161 gaacctttat tcgtgattgc tcctgcaatt gctccgataa ttcctccgac gataagtgac
     2221 caaataaatc ccatgtttcg ttcctcctaa tgttttactt ccttattact gaacccgtgg
     2281 ttctgtattt tcttttactt tttgtgcgcc gcttccagct gctgatttta ctttatctgt
     2341 ttgattcgaa gcaaattcac cggttttttt cgctgcgtca gttactttgt cttgcaccgt
     2401 ttcttgatct ttttcaaatt cttcttttgt tttgatgtcc actacgttag cgttgacttc
     2461 gatcacttct aaatgtgtca tattttttac ttctttgccg attacttctt gcatttcttc
     2521 aaagattttt tcgatatctt tgccatattc cacaatgaca tccatatcaa ctgctacttg
     2581 ttttttaccg acttctgttt cgatcccagc cgttacatta tctgtattca caagttttcc
     2641 tgccacatta gagaagaaac caccgtttgc agataataaa ccgtctactt gttcaattgc
     2701 gattccgatg attttttgca caactttatc ttcataattc agctcaccgc gcattgaatc
     2761 ttcctgttca gaatcatgct ttccttggtt atttgtatga tcagaaccaa gttttccttg
     2821 tccgtttgtg tggtcagaac taagtttttc ttgtgctttc gcatggtcag aaggatgttt
     2881 ttgctcttcc acaggtttag aaggatcgat tgcatcatgt tgttctggaa ctttagattc
     2941 tggagttcct ggatgatgag gtgcatgctt ttctgcatca tgtttcttct cttctactcc
     3001 ttgttgaacg ttagggacta ctggatctgg atgtgcagat actggtgctg ttttgttctt
     3061 ttcatcgttc acaccatttg attggtttgg tgtcgttggg tctggatgtg cagacactgg
     3121 tttttggttg ttcattttat tgttattatt tttgttattg ttattcatgg atgtttcctc
     3181 ctaagattta aaatagtgat ctaagatttt cgtttgtttc agataaaaac cgatagcgac
     3241 gccgatcgct gccatgatca agatcaataa ggtcttgaaa aagcccacgg tgatccataa
     3301 aacggctaat atcaatccaa gtaccccaaa gatgataggg agcttgtatg tcgaaaatag
     3361 ttctttcatt tttggtcccc ccctattcta ctcgtttgcg gtcattgctt cgtttagctg
     3421 gttcttcaaa gcctgtgtat ttcaccgata ctttcactgg ctcttttgtt cccaatatgt
     3481 gttgtacttc attttcgatt tctttcatca atgttcctgt tttaccaatg agtgctgaag
     3541 ttcgtttcaa ctcccctttt acattgacgg cgatcttgtt tttcgttgct ttgacattta
     3601 cttttggtga atggatgaat tcatcttcat gcaagtgaga acgaaccatt ccttcaattg
     3661 cttttttatc caacgtcaac tccccatcac catgtttcag tagaaagttt ccgattgttt
     3721 ttggataaaa caggaaaaca agtaacgtga taattgtcaa aatagcaaat gcaacggcaa
     3781 gccaaaatac atattgttgc acgtatgaat tagtgatgga aaagaatcga aacttctcta
     3841 gctgaaaagg caatggatag gttgcactta atagtatcgc aaacaaaagc acagatagca
     3901 aaagtaatgt tccgatcact ccgatcgttt ttacaccttt attcattttc gcacctcctc
     3961 ataggggtga aacgatcaac ttcttataag ttgatcgctt ctccaccagt tactccgtaa
     4021 atttgggctg taacataact tgctttattc gatgctaaga aaacataaac tggtgctaat
     4081 tctcctggct gacctgcacg ttctagtaaa ctttgctgac caaattcagg gatcgaatca
     4141 gaaggttgtc cttcgtctag ttgtaatggt gtccagattg gaccaggtgc tacaccattt
     4201 acacggattc ctttaggtgc aagctgtttt gctaaaccga tcgtaaagtt agcgattgaa
     4261 gcttttgttg ctgcgtaatc taataaatgt tcgcttggat tgaatgcttg aacagaagtt
     4321 gttgttacga ttgctcctcc tgctgggata tgcggaacag cttctttcac taaagcaaac
     4381 atagcgatga tgtttatcat aaatgtgtct ttgacttgct caagtggtaa ctcagcaata
     4441 gactgacaag aaatttgctg tcctgcgttt agtaccatcg tatctagccc gccaagttca
     4501 ctcacggctt tatctatgat ttctttaggt gcttgttcgt cacgaagatc aaaaggtagt
     4561 aagacaatct tacgtcctgc atctttcacg tattttgcta cttcctctgc atctttttct
     4621 tctcctggga agaattgaag agcgatatct gccccttctc tagcaaatgc aatcgctgct
     4681 gcacgaccga tccctgaatc tccaccagtg attagtacac ggcggttttc tagttgatta
     4741 ttgccttcat aggattcttc tccacaatca ggaacaggaa tcatattctg ctgtaaagct
     4801 ggtgtatcct gatcttgttt aggaaatttc tccgtatggt acaagtttcg cgggtcgatt
     4861 aattttggtt ctgtcattgc tgtattcctc ctagttttta aattgtattg ccaaaacaaa
     4921 tttctttgtt tgatacctgt tttaaataga ttctttaatt tgtataatct ttccggtata
     4981 aatggtaacc taaaactact gctgctatca aagagccaaa gactacgagt ataaagccta
     5041 gagcagaact tttacccggt aatcctcctg tattcattcc ccaaatccct gcgaacaacg
     5101 ccggaacgga aatgattaat gcagctgaat ctagatattt catgatatga tttagattat
     5161 tgtccatcat cccgttgaat agatcaccaa tactctctaa taaatctcga tagatctcga
     5221 tcattttttc tgtttgtcgc tgtctcaaat ggacatcata gagtagctgt tcatcgtcta
     5281 aacgtttgat gaaagtttca gaattccata attgatccat tgtttcccgt tgatctctta
     5341 aggtatgatc gatatatacg atgtctcttt caagatctgc ctgtttatac agttcttcat
     5401 tttccgttgt tttacgagct gctttatcca aagcgtcaat tcgctttttc atctctctta
     5461 gttctttcac gtaatgggtg taaatatcta ataatgtata cgtgatgatc ttttcaaacg
     5521 agtttaaatg atttccatgc tttgtcagca gccgatccac aaattcggtt tttctatcga
     5581 tacaagtaat caagagatta tcggaaagta caaaagatat tggcgttaat tttttttcga
     5641 tacgattatc tgactgatct agattaatca ctaccaaaga aaatggatgc tctagtttcg
     5701 tctcataaag gcgctccatc cgagaaactt cttccggata ttcggttcct acgaaaatat
     5761 catcaggaag ctcgaattct tcgattgctt gctcaatctc ttcgggcttt gcactatcaa
     5821 gtacgagcca attatatttt ttttcatctg tttctattac attcttttct tcatccacaa
     5881 agtagtatct caatgtatac ctcctttttg gactttctcg aaactattag ctgaagaagc
     5941 agttctttaa tttgatatta taatcgagta ctgcgatttc attttctaat gaaaagctgt
     6001 caagctgttt gttgaagtcc atgttcaatc gtttaccgaa ataattttgc tctaatttag
     6061 atgtatagat gtccaataat ggatgctcga ttttagtcgc atattctcca aattccgaaa
     6121 caaaaggaat gatttgaaaa aagctgcctt ttgcaaaagc acctttgtcc cttagaaatg
     6181 gatcggaatc attgataagc gcgagtgcga tttttgtatt ttccccaacc ttttcaatta
     6241 acgtctctac tgcattttta gacattgtaa tttccattcc tactccccct cttcagttgt
     6301 tgtcttctat tatttaacaa ctacatctgt ttttggttga acttttgcat attttggata
     6361 tttagggaat ttagctacac aacggaaagc cgtcatccct gcatattttt cgtaatattt
     6421 cttcgttggc ccttcaaaat ccttgctcca cggtttcaca tgtcctgaaa aatggataat
     6481 gtaaggattg ttgcgagttt cttcgtattc acgttcacct gctgctgttg gatgtttttt
     6541 tgcttttgcc atgatatagc cttgtgcatt ccaacgcgga tgaagtggta accaacggtc
     6601 atgtaagata gcatttaatg catcttggtc atggaatcgt aatttttctg gattatgttc
     6661 gatgaaatct aatacttttt gcgtaatatt ttcatctaac cattttttca cattgatcag
     6721 catcaaacct gagttgaaat aacgcattga tttagctgaa attttcattt tttccagtcg
     6781 ttgatggaac ccagcgtctt caacagctgc tacgattgaa tcaccgaagt caagacgcca
     6841 taatttagag atatcttgta aagcgatcat gtcgcaatcc atatataaga tacgttcaac
     6901 ttctgtgccg cggaacaatt ccggaatcgc aatacgataa taagctgttt ctggaatacg
     6961 atcgctgatt accacatttg taaagaattc tttattgatt ttcaagaatt ccacatctga
     7021 gttcattcgt gcgtttttca ctgagaagcg taaaagctgt ttactttcaa aatcgatatc
     7081 atcgtcaatc acatagaacc gaacaaattt agacgggttg caattatcta atactgagac
     7141 aaataatgca gctaaatgtg gtacgaattt tgtgttacaa cttgcgacta ctgctatttc
     7201 ttttttattc attgatctcg cctactttcg ttagagtact gtgtgctaat ttttttaagt
     7261 ataaattggt atacggatga tctttcaacg tattccaagg tttatcatgt cctgtaaagt
     7321 gaacgatcga aggtttttca tttccttctt tatactgacg ttcatatttt tcatttggtg
     7381 ctggatgacg ttcaaaaatc agcgaagtct gcatgttcca ttttggatga agttgttccc
     7441 aatcttcata aagtacagca ttcaaagcat cttggtcgtg atagatgatt cgatcgccat
     7501 tttcactgag gtaatggata gttttttctg taatttcttt tttattccat tgatcaatgt
     7561 caatcaccat cactcctgaa ttgaagtaca agtcatcgga atcgattcct aatctttcca
     7621 aagctttcac ttggcctgga tcgataatag cgccgattgt ttttccattt aacggctcat
     7681 catatagttt tacgatatcg tctagtacta acacatctga atctaagtaa aggactttct
     7741 tataatcctc tttagctaat aagtttggta aagaaattct taagtaagcg gtagttgtga
     7801 tatggtcact gactaggaag tcttcgtaga tatctttttc cactgtcagg aattggatac
     7861 ttgcattact tgaatattta tttaccgttt cttccaatcc ttctttacta tattctgata
     7921 gaccatcgtc aatcacataa aattttatgc gtcttgtttt attgcaattt tctaatgcag
     7981 ttgcgatcat cacgctcaag taaggagcat aattttcgtc tgaagcagtt accacaggaa
     8041 ctacgccgta tctcttctcc atcaaaatca ctcctttttc taatcttttt ctaacctgtt
     8101 ctacatctag aatattcctc atttggtttt aaagcaaaat atatgctttg ttttcattga
     8161 atattttaaa atttttttat cacaaaacgc atcggtaagc gctaactatt cgtttttcat
     8221 ggctttttat tcaaaggtat tgaccaaaaa ctactatata tagccacctt tttcttaaga
     8281 atgttttttg aaaaaattca taatgacaaa gcaagttagt ttcatttttt taaattctca
     8341 tttaatctta aggtaacagg aaaatagata tgtaaaaaga aagtaggaag ataaaatgaa
     8401 actagcagcg attgatttag acggtacctt gcttgatcga aatggaaaga taccagaaga
     8461 aaatatccaa gcacttcgga aatttgatga aaatggcgga atcgtaaccg tcgctaccgg
     8521 aaggaacagc atttctgcta aagatatttt ttccgaatta ggaatcggtg gctatttgat
     8581 ttcttccaat ggttcactga ttgctgaaat gaaggatgga aaaatggcga aagttctcag
     8641 acgttcaaaa atagatagcg atacactgag aaaagccttc ttctatgctc aagaagcgaa
     8701 aatatccatc attgctagcc gagaaaccca agatgatcag ctgacattcg atgaatcaaa
     8761 tctagtcaaa gatgaccctt attatgaaca tttcaattta cagaaccatt cttttgatca
     8821 aattttaaat atgttggaag atccttctct tagctatctt aaattagcat taacagataa
     8881 gagtgaagaa aaactacaga agctacaaag agatctgcaa gcagatggta tccaatcagt
     8941 attctctgat ccgcatttct tagaaatcac gcctacaggt atcactaaag ctcattcact
     9001 tgtttttctg gcggattacc ttggaatcga atcgagtgac atcatggctt ttggcgatca
     9061 agaaaacgac attgctatgt tgaattacag cgggttgagt attgcgatgg ggaatgcaca
     9121 gcgacaagta aaagaaattg caaaccaagt aacactgacg aatgaagaag ctggtgttgc
     9181 ttatatttta aaaaacttct attaaaaaga ataaaatgac gatatgaatg taggaggaaa
     9241 gaaaatgtta gttattattg atatgcaaaa tcatattctt gatccaaaca gcgaatttta
     9301 tattccagag tcagatcaat tggcagaacg tattgctcaa agattagcta aagcacgcca
     9361 aaataatgaa tatattctct tcacacggga tataccagtt gaactgaagg acaaagaaga
     9421 agaacgagaa gatttgcaat tgatccctct tctttctcca caagaaaatg aacgagtgat
     9481 taaaaagtat tactttacga ttccacctga aactttagct gaaatccagg aaactttatt
     9541 tgaaagcaaa gaagaacaaa aagagatcga agtagtcggg attgaaacaa acttgtgtgt
     9601 actctctaat ctgataggcc tgcagagtgc ttttcctgaa gcagactttt ttgttgatcc
     9661 aatgttagtc tccagtagaa aacacggaga atccgcttta gagctgttga aagatttcaa
     9721 cgtctctatc gtcgaatcat aaaaatatct atcttcattt acgatattta acacacaata
     9781 aaaaaagcac gcagaactgt tttagtccta cgtgcttttt ctatcaaagt agattggctt
     9841 ttttatgaca cagagtgaaa cggcaggctc acgacctctt tcttaaatgg tgttcaatca
     9901 gctgaccctc ggtattagct taacaaacgg caaaatatga ggaactattt ttccgtttgt
     9961 taagctaata ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac ggtattcaaa
    10021 agcttgcatc tgcagtaata agaacagcca agtaaaaaca taagaagctg tttttacttg
    10081 gctgttctta ttacttgatg cagggcagct ttttcacaac ctcttatgta ccttgtagga
    10141 ctcgaa
//
LOCUS       DAZKOU010000099         9996 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000099 DAZKOU010000000
VERSION     DAZKOU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.100"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..1265
                     /locus_tag="ACG827_002444"
     CDS             93..1265
                     /locus_tag="ACG827_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0484551.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            1521..1721
                     /locus_tag="ACG827_002445"
                     /pseudo
     CDS             1521..1721
                     /locus_tag="ACG827_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2103..2249
                     /locus_tag="ACG827_002446"
     CDS             2103..2249
                     /locus_tag="ACG827_002446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484552.1"
                     /translation="MELYFSGISQRTISLTVGSSRHTIREVTSRADRKGVTELTADMS
                     DTWL"
     gene            complement(2433..3038)
                     /locus_tag="ACG827_002447"
     CDS             complement(2433..3038)
                     /locus_tag="ACG827_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0484553.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            3220..3555
                     /locus_tag="ACG827_002448"
                     /pseudo
     CDS             3220..3555
                     /locus_tag="ACG827_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3720..3935
                     /locus_tag="ACG827_002449"
                     /pseudo
     CDS             3720..3935
                     /locus_tag="ACG827_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4073..5044
                     /locus_tag="ACG827_002450"
     CDS             4073..5044
                     /locus_tag="ACG827_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0484554.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            5184..6497
                     /locus_tag="ACG827_002451"
     CDS             5184..6497
                     /locus_tag="ACG827_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFQ0484555.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            6540..7406
                     /locus_tag="ACG827_002452"
     CDS             6540..7406
                     /locus_tag="ACG827_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0484556.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            7403..8251
                     /locus_tag="ACG827_002453"
     CDS             7403..8251
                     /locus_tag="ACG827_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0484557.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            8265..9737
                     /locus_tag="ACG827_002454"
     CDS             8265..9737
                     /locus_tag="ACG827_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0484558.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9754..>9996
                     /locus_tag="ACG827_002455"
     CDS             9754..>9996
                     /locus_tag="ACG827_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0484559.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         3171 a         1740 c         2058 g         3027 t
ORIGIN      
        1 ccatataatt gtgtaaaaga taaaaggcca tgtaacagcc cttttacggt acaatgtttt
       61 taaccacaaa aacataccca ggaggacgtt acatgaccca agtacatttt acactgaaca
      121 acgaagaggt tcaaagtatt attgaacatt cggtaaaaga tgatgtttct aaaaatattt
      181 taaccactgt tttcaaccaa ttgatggaaa atcaacgaac agaatatatt caagccgatg
      241 actatgaacg ttcagaaagt cgtcagagtc aaagaaatgg ctattatgag cgagacttta
      301 cgactcgtgt gggtacactc gaattaaaag tgcctagaac acgtgatggt gaattttcac
      361 cgacggtgtt tgagcgttat cagcgaaatg aaaaggcact gctcgcttca atgcttgaga
      421 tgtatgtttc gggcgtttcg acacgtaaag tttcaaagat tgttgaagag ctatgtggaa
      481 aatctgtatc gaaatctttt gtttctagcc tgactgagca gttagacccg atggtcaacg
      541 aatggcagaa ccgttcactc tcaggtacga attatcctta tctgatgact gatgttctct
      601 acataaaagt ccgtgaggac catcgagtgc tttctaaaag ctgccatatt gcgatcggga
      661 taacagaagg tggcgaccgt gaaatcattg gcttcatgat tcaaaatgaa gaaagtgatg
      721 acacatggtc catcttcttt gaatacttaa aagaacgcgg cctaaagggg acagaactca
      781 tcatttctga tgcccataaa ggcctagtgt ctgcgattcg taagtcattt accaacgcaa
      841 gttggcagag atgccaggtc cattttttaa gaaacatctt cagttccatt ccaaaaaaga
      901 attcaaaacc gtttagagaa gcagtaaaag cgatctttaa gtttacggat attgaactcg
      961 ctcgaacagc taagaatgcc ttagtcggtg aatatatcga ccagcctaaa tatacaaaag
     1021 cctgcgaaat attggataat ggcttcgaag atgcctttca atacacggtt atcggaaata
     1081 gtcataatcg gctaaaaagc accaaccttc ttgaacgact gaaccaggaa gtccgcagaa
     1141 gagaaaagat tattcggatt tttcccaacc gaacgtctgc caatcgatta attggagctg
     1201 tccttatgga ccttcatgac gaatggctca gttctacaag aaaatatatt aagtttgatc
     1261 aatgagagac cggtaaaaca ttgtatagta ttttacacag gattatggac ttgacttgag
     1321 aacaccacgc atagttttta ttttgagata ttctagaatg aaacacatta agaacgaaag
     1381 caaagcaaaa gacgccagaa cttctactac cttctaagtt ttttcatatt tgtttgttat
     1441 gctatacata gatctgcata attagacttg cgctgcccgt tattgaagtt tcaggcagcg
     1501 ctctttttgg gggatcgata atggaagaag aaaaaatatt tgagaaacgc tggcagctag
     1561 catcaagtga acaaagagca agatacaata atttgatcac ctcctacccc actgttgact
     1621 agacatataa ggaaaagaaa tacttacttt ggctgtgcca gttagatata gatacctttg
     1681 aaacatttga agtgatccta gataaaatta aacagaattg atcagaaaca agcgaagcct
     1741 tctctcggtt cgcttgtttt gctatttatt agttggctca gggcaactct aagctcgttt
     1801 gaaagaaaac cgatggataa tccccttgtt tgtaccactt tagaatacat ggatgtacgt
     1861 attgccattg actcctatta gttactgaaa aaaaatttaa acaagttgag tattgcagat
     1921 aaatagctcc agtgaggtga ataactgctc cactcaaggc aatagatagc tccatccagg
     1981 gcaacaaact gctccacttt catatttaat agatgaataa ccttctgtat aatagaaagg
     2041 catacctttc ggtatgacaa cttatacaga atgttatttt attggttcac tatcgaaaaa
     2101 ttttggagct ttatttttcc ggtatttctc aacgaacgat tagtttaact gtgggaagct
     2161 ccagacatac gattcgagaa gttacttctc gagcagacag gaagggggtg actgaactaa
     2221 ccgctgatat gagcgatact tggctgtagg aatatctttt tccggaaaaa caaacaacag
     2281 cacgaggtta ccttcctgta gactgggaat atgttcataa agaactaatg aaaaagaata
     2341 tgacattgaa actccttcat agagaatatg gggtacatgg aacatagaaa cgaaaatgca
     2401 cggtagaaaa gcgaatgaat atagtataaa ttctacaaat cattcgcttt ctttatttta
     2461 tatatggttg gtcgagagag accattttct ttggcaattt ctgcaactgg aactccttga
     2521 tttaacatgt ccaccacagt tttaaagact aatcgttttt gcggatctgt tgagttctca
     2581 gaatattcaa ttggtcgccc cttatatcga ccagcttttt tggccaataa tactccttgc
     2641 cgctgacgtt cacggatttt ctcacgttcg ttttgtgcaa tataacttag caatgataaa
     2701 aacatatcga acatagcagt atctaggagt tcatttccag tattaaaatc taaaaactgt
     2761 gcatctagta tttttaatgc tacttttttc tgtttcataa aggtaactgt attttttatg
     2821 tcttcataat ctctgcctag gcgatccagt gatgcaacaa tgacacaatc accttctcgg
     2881 atataattta gtaatttttg aaactcaggt cgctggatat ctttaccact ggctttttct
     2941 tggaataatt tatcaatgtt aaggtcttcc atcattgcca attgtcgatc taaattttga
     3001 tcttgtgaag acactctaat atatccaatt tttgccataa aaactccttt taaaaaatga
     3061 tataatttac atgtaaaatt tacttgtagt tttactgtta tcatagcatt tttaaaaata
     3121 aattttaagt ctacttataa tttactttgg aggttttgaa tgaatgaaat taagcgcaat
     3181 aaaaattctt ttggtgttta tttaggagag cctagctcgg aacagttaaa taaaggtgaa
     3241 gctcgtcatg ctctatgtcg aaatatattt tatggtagaa aaggcaaact ttatcaaact
     3301 tattacgacg gtatggagga acagctcaac gcattaggat tagtaactaa tgctatcata
     3361 tattggaatt cactatattt agaaaaagtc tttgaacaaa ttaaaacgga agggttcgac
     3421 tgttccgaag aagtgttacg caagcaattt ccattaatta gtgatcacat taattttgtt
     3481 ggcaaatata gtttccgcta tgaaaaagaa ttatcgaatg gaatactacg tccgttaaat
     3541 atagagaatt attgagaatg ttcgtctttt ggttcctacc gtgcattttc gcacggatgt
     3601 tccagatact atttttgaat gtataaaatt gaaaacacaa gaatactaat aaatcaacga
     3661 ttcttgtgtt tttaattttt cactaccgtg cattttcgtt tctatgttcc atgtacccca
     3721 atcttgaaaa attaattaaa gacttagata aagccattga cgacttagtc gtggtgctac
     3781 cggagtatca atgtttaacc agtatcccag gtattggtcc tgtttaggct gctggaatac
     3841 tcgctgaaat tggtccaatt gaacgttttt ctgatgaaat aaaattgaca aagtatgccg
     3901 gcctatattg gcgacagagt caatccggta aatctgaata cgaaaatttt tttacgaaaa
     3961 gcttcacgtc taatatctat tttaaaataa aaatattcct gctaatttgg aatcgtttcc
     4021 ttcttttggt ataataatac ctgaaattct tttgagagga gttgttcgtg aagtgaaaac
     4081 tactatgaag gatgttgctg cgttagcagg agttggtgtg ggtacggtat ctagagtaat
     4141 aaatggagta aaggtgaaag aggttaccaa agaaaaagta atggctgcaa ttagtgagct
     4201 taattatgag cctgatgaat atgccagagg tatgaaaacg aatagtagca atacaatagc
     4261 tcttatttta cctacgattt ggcatccctt cttttccgag tttgcctact atgtagagga
     4321 agctctcagc caaaaaaatt ataagctttt tttgtgtaat gcgaacggca gtgcggaaaa
     4381 agaaaaagaa tatatccaaa tggtcaagca gagtaaagta gacggaatta taggtattac
     4441 ctattcagac attgataaat atgtatcatc aaatttacca tttgtcagta tagaccgcca
     4501 tttttcggaa caggtttctt atgtaacggc agataattat ttaggtgggc aaattgccgc
     4561 tcaagaacta cttactcgta actgtaaaaa cttgtgctat gttgggggtg tttcacctta
     4621 ccctaacgaa acaaacaatc gtaaagctgg tttttacaat atttgtgagg aaaataaaat
     4681 agctgtgaag gtattggata tgcctgaacc aattagcaat ttagaggaac aattgctcgc
     4741 ttttttttcg gataatcctg aaattgatag catttttacc atcaatgatt tcatggcttt
     4801 ggatgtgata aaagcccaaa aaaaactagg gaaggaagca cctaaggatt ttcaaattat
     4861 tggttttgat ggagtcaagc tatcgttgca aaaagactat ttactatcaa cgattgtaca
     4921 gccgatagag gaaatggcca aggtttcagt agaaacactc atggcgatga ttttaaatga
     4981 agaaccgcct aagcgtgtgg tagtaccggt tttctttcac gctggagaga cgactaaaga
     5041 gtaaaaaacc ggtttataac gatttttttg agataggttg acagaaagtg atttcaatga
     5101 tatcttaaaa ccaattaatt ggaaacgtta ccaattaatt gaaaaataaa acggaaacgt
     5161 ttcaaaaaat aggaggaaca tcgatgaaaa agagaatgtt agcaattgca gcaattttag
     5221 cattaacagc tggaggactt gcaggatgtg gcagtaagga gaaaagttca ggtagcagtg
     5281 atccaggaga aattaagatt tgggttcagt tttctgatga aacgcctgaa ggaaaagctt
     5341 gggcagagat tgtgaacaat tttaatgatg aatacgaggg tgaatataaa gctgttactg
     5401 agtatattcc tcgtagcgga agcggcggtg gatatgagga taaagtaaat gctgcaatta
     5461 caaccaatac ccttcctgat gttattacgt tagatggacc gaatactgca gcttatgctc
     5521 attcaggagt cattgctcca ttagatgatt atttgaaaga tgcggacatg gatgatgtat
     5581 tagacagcat tattcagcaa ggaacctacg atggcaaaat gtatgcgttt ggtttctcag
     5641 agtccagtgt gggtgtctat tacaataagt cgctgttcaa agatgctgga attacagaag
     5701 atcaattgcc gacgttagaa gatccttgga cttggacgga atttcaagca atttgccaaa
     5761 agctgactga taaatacaaa acgcctgcaa ttgatatgca aatggcgtcg ggagacgaaa
     5821 tgctgaccta cgcatataca ccatttatat ggtcgaatgg cggcgatgtc gttaatgctg
     5881 atggaaccaa agccgaagac tattttaacg gtaaaaaatc cgttgaagcg ttggaattta
     5941 ttcaagaatt ggttgaaaaa ggttacacca caatcagtcc tattgaaaaa ggatttgaaa
     6001 ctggcaaata tgccatgatg ctgagcggtt cttggacaat ggcagattta gaggaaaact
     6061 atcaagagat tgattatggt attttgcctt accccgtttc agatgcaact ggggagttag
     6121 tttcaccaac cggaagctgg cagttagcaa tgactgagaa aacagagaaa aaggatgctt
     6181 ctgcggcctt tattaagttt gcaaccagta cagaatctag tcgtattatg agtttaggga
     6241 acagtgttct acctatcaga aattcaacag ttgatttaat taaagatgaa gtgtcggaag
     6301 gaatgcaggt attaattaag caaaaccaac tttctggaca tgcccgccca gtggttgtag
     6361 cttatccgca agtatctaga gcattccaac aagcagttca agatattagc tacttcgagg
     6421 aaaacccaga tgtacaaaaa gtagcagata gtcgcgctgc tgaaatgcaa caggcaattg
     6481 atacttcact aaaatagaag tttctaaagg gggccaatca agtgttaaaa aaaccgacaa
     6541 tgaaagacaa tttgataggt tacggctttc tagcgccagc tttgctgctg ctgacgattt
     6601 ttctgatcat acccgtaatc atggtaattt attatgcctt tactgattat tatttgttaa
     6661 cgccagatgc tagacaattt atcggcttag aaaattttaa gcgactgttc gctgatccaa
     6721 tttttgtaaa aagtatttgg aacacggcta aatttgttgt ttggattatt ccggtccaaa
     6781 taggggcagc tttaggaatg gcacttatta tcaacaaaaa tcgtaaagga aatatctttt
     6841 ttaaagtagc cttttttgcg ccagtcgtga tgtccttagt cgttatttcg attttatggt
     6901 tgtacttgtt aaatcctaat gaaggtctgg ttaatacgct atttgcaaag attggaattg
     6961 cttcgcagcc ttttttgact agtcccaaac aagcaatgta tacgcttgtt ttcgtatcgg
     7021 catggcaagg tgcaggatat cagatgctgc tgtttttagg gggaatgcaa aatattccgc
     7081 aagacgttta tgaggcagcc gaaattgatg gtttttctaa gtttcagcaa tttcgctata
     7141 tcaccatgcc attattaaag cccacggcga tttttatttt acttaccaca ttgatttcgg
     7201 cctttaaact aatcattcag ccaatggtaa tgactcaagg tggaccaatg aattctacta
     7261 tgacaatggt ttattacatt taccaaactg gatttacaga ccgtatggta ggctactcca
     7321 gctccattgc gttaatcttt accactatga ttggtttaat tacaattgct caaagaaaat
     7381 tggtaaagga ggatgatcca gcatgaccaa gaaatttgga ccgctaatga tcgtagaata
     7441 ttgtttgctg ttccttttag cggcattatt cattttcccg atgctgtgga tggtagtttc
     7501 ctctatgaag cctgaagcag atgtctatca aaatttagct tccttcaaag cttttttacc
     7561 tagtttgaat ccagctaatt ggtttaaaac ttatcaagag gttatttccc ggttcagtgt
     7621 gggaacttat ttattaaaca gtatttttta tggtttaacc tttgcctgtg ggtcaatcat
     7681 tgtaaactct ttggcgggtt ttgcctttgc gaaggttaat ttttcaggca agaaaattat
     7741 ctttggagct ttgttagctc tgctgattgt cccgatggag acggttttaa ttccgcaatt
     7801 taccatcgtt aattcactag gcttagtgaa tactcgcttg gcagtgattt taccagcgat
     7861 ggccagcgcg tttaatatct atctgtttag aaatttcttt atcgcgattc cagaagaaat
     7921 cattgaatca gcgaaattag atggtgcaaa tattgttaaa attttctttc acatcatgct
     7981 gccaatgtct aaaccagcca tagccacagt aggtgtctta tcctttatct ccagctggaa
     8041 tgattatatt tggccgttaa tggtcttaac tgacagcagc aaattttcaa tgcaagtggc
     8101 aattactacg atcaatacta cacagccagt ttatatcaat caagtgatgg ctgttttaac
     8161 cattgcaacg attccactga ttattgttta tatcgttgca caaaaatata ttctgcaggg
     8221 acttggcggt tctggaacgg gtataaaata ggagtgaata gtgaatgttg caaaaacttt
     8281 taaaggccaa tgagtttatt gagaaaaatg ctcaaacagt caatcaagaa taccggaata
     8341 aatttcattt gatggctccg attggctgga tgaatgatcc aaatggtttt gtttattatc
     8401 aagaggagta tcacttgttt tatcaatatt atccttacga cagcatctgg ggaccaatgc
     8461 attggggcca tgctaaaagc aaggatctaa ttcactggga gcatttgcca gtagcactgg
     8521 caccaggtga aagctatgac aaggaaggct gtttttccgg cagtgccata gaaaaggatg
     8581 gcaagctgta tttgatttat acaggacaca atgttgtgga agggcaagtt cgacaagttc
     8641 agtgtttggc agtttcagaa gatggcattc actttgaaaa atatgccgga aatccaatca
     8701 tcgcagaaga tcacttagct tctgttgcaa cgaccgaaga tttccgcgat cccaaagtgt
     8761 ttcagcgaaa agaagactat tttactgtag tcgctactaa aactgcagat gctcgtggac
     8821 aaattgtgat gttccattcc aaagatttac taacttggga gttttattcc gtattgctgg
     8881 agggaactcc agaacaaggc attgtatggg aatgcccaga cttgtttgaa ctagatggca
     8941 aagaagtctt gattctttct cctattcaaa tgactccaca aggcaatgct taccaaaata
     9001 cgaactcaac tgttgctttc atcggtactg tcgattggca aacaggcaaa tttctagtag
     9061 agaattttca tgaaatcgat ggcggtttag atttttatgc accccagacc tgtgaaaacg
     9121 aagcaggcca aagaataatg attgcttgga tgcaaatgtg gcagcgtaaa atacctacga
     9181 atgatctggg ccatggttgg tcaggttcca tgaccttgcc tagagagctg agggtggctg
     9241 ataatcaatt gttgcaacgg ccagtttcat caatttatca agtaattgat ccgcaggaaa
     9301 ggattgaatg ggcgaagact gaagagaatc gcttcttact gaaggactta gtatatgacc
     9361 agcactattt acgcttggaa atcaatttaa aacaggcaac gaaactagaa cttcactatg
     9421 cgaagggacc cgaccataca ttactgctta actatgatgt tgaacgggag tgctttactg
     9481 cttccaggga acacatgccg tatgatttat caggagctga aaaggaagtg ttgaaaaagc
     9541 gcagtgtgac gattcctttg gaaaataatt gtctaaccat cgaaattttc cgtgatactt
     9601 cagctattga gctttttcta aatgatcgtc tgactatgag tacaactttt tatgagacag
     9661 aaaaaggaca ggattttctt ttgctggcag acagtgaagt aaccttttca ttggaaagcg
     9721 gcttaattaa tctatagaag ggagagctca gtgatgattt atggttcaat cgaagcaggc
     9781 ggcacaaaat ttgtgtgtgc cattagcgat gaagaaatga cgatcaagga aagagtgagc
     9841 tttccgacaa cgacacctga ggaaacaata cctttagtca ttgacttctt caaacaatac
     9901 caagcagatt tagcagggat tgggattggc tcatttgggc ctatcgatat ccatcgcgat
     9961 tccgccactt atgggtacat tacctcgacc ccgaaa
//
LOCUS       DAZKOU010000100         9811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000100 DAZKOU010000000
VERSION     DAZKOU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.101"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2154)
                     /locus_tag="ACG827_002456"
     CDS             complement(34..2154)
                     /locus_tag="ACG827_002456"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0484560.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ACG827_002457"
     CDS             2384..3562
                     /locus_tag="ACG827_002457"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0484561.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ACG827_002458"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ACG827_002458"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0484562.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG827_002459"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG827_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0484563.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ACG827_002460"
     CDS             complement(4915..6168)
                     /locus_tag="ACG827_002460"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0484564.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG827_002461"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG827_002461"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0484565.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ACG827_002462"
     CDS             7746..9092
                     /locus_tag="ACG827_002462"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0484566.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="ACG827_002463"
     CDS             complement(9400..9786)
                     /locus_tag="ACG827_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0484567.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DAZKOU010000101         9465 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000101 DAZKOU010000000
VERSION     DAZKOU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.102"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG827_002464"
     tRNA            2..74
                     /locus_tag="ACG827_002464"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG827_002465"
     tRNA            78..150
                     /locus_tag="ACG827_002465"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG827_002466"
     tRNA            155..225
                     /locus_tag="ACG827_002466"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG827_002467"
     tRNA            238..311
                     /locus_tag="ACG827_002467"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG827_002468"
     tRNA            323..394
                     /locus_tag="ACG827_002468"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACG827_002469"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACG827_002469"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0484568.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACG827_002470"
     CDS             1983..3455
                     /locus_tag="ACG827_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0484569.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACG827_002471"
     CDS             3538..4326
                     /locus_tag="ACG827_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0484570.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACG827_002472"
     CDS             complement(4393..5061)
                     /locus_tag="ACG827_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0484571.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACG827_002473"
     CDS             5176..6888
                     /locus_tag="ACG827_002473"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484572.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACG827_002474"
     CDS             6891..8663
                     /locus_tag="ACG827_002474"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484573.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..>9465)
                     /locus_tag="ACG827_002475"
     CDS             complement(8745..>9465)
                     /locus_tag="ACG827_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0484574.1"
                     /translation="KTFTGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGY
                     LSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTY
                     DYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISI
                     QAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKI
                     YTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         2843 a         1585 c         2047 g         2990 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc tcttaaagat tgattatatg cttgaataga tatcgggtta
     9001 ccatttttag agatgaaaat gtatttatct ttattattct taattaattt gttttcttca
     9061 attaattgat ctatgatttc tttagcatta cttgataatt caatggttct ataagaacct
     9121 gtatttttag ttgttgtttt aatttttaac tcgtaatcat aagtaccaga aaccgtaatt
     9181 ttattaccat cataatcaga ttcagtcaaa gatatcaatt caccataacg tagtccagtt
     9241 aaaaacaaaa atttagtaat taaagaataa cgtgactgtt gatgtgaact tttttgttta
     9301 ctagcaatca tttgaacttc ttcttttgat aaataaccat ctttttcttt ttttatttct
     9361 tcgtttgtct taggttttct tgctaaagaa accccattta cgggattata ttttaatcca
     9421 tatcgacggt tggcgaattt aaaaatcatt cctgtaaaag ttttt
//
LOCUS       DAZKOU010000102         9432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000102 DAZKOU010000000
VERSION     DAZKOU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.103"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..680
                     /locus_tag="ACG827_002476"
     CDS             <1..680
                     /locus_tag="ACG827_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0484575.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="ACG827_002477"
     CDS             800..1000
                     /locus_tag="ACG827_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0484576.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="ACG827_002478"
     CDS             1076..1273
                     /locus_tag="ACG827_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484577.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="ACG827_002479"
     CDS             complement(1570..3117)
                     /locus_tag="ACG827_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0484578.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="ACG827_002480"
     CDS             complement(3201..4004)
                     /locus_tag="ACG827_002480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0484579.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACG827_002481"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACG827_002481"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0484580.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACG827_002482"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACG827_002482"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0484581.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACG827_002483"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACG827_002483"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484582.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="ACG827_002484"
     CDS             complement(6389..7318)
                     /locus_tag="ACG827_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0484583.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="ACG827_002485"
     CDS             7513..8196
                     /locus_tag="ACG827_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFQ0484584.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="ACG827_002486"
     CDS             8357..9400
                     /locus_tag="ACG827_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0484585.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DAZKOU010000103         9303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000103 DAZKOU010000000
VERSION     DAZKOU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.104"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG827_002487"
     CDS             <1..435
                     /locus_tag="ACG827_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0484586.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG827_002488"
     tRNA            complement(759..842)
                     /locus_tag="ACG827_002488"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG827_002489"
     CDS             1370..1870
                     /locus_tag="ACG827_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0484587.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG827_002490"
     CDS             complement(2060..2404)
                     /locus_tag="ACG827_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0484588.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACG827_002491"
     CDS             complement(2558..3478)
                     /locus_tag="ACG827_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0484589.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACG827_002492"
     CDS             3665..4156
                     /locus_tag="ACG827_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0484590.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACG827_002493"
     CDS             complement(4180..6555)
                     /locus_tag="ACG827_002493"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0484591.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACG827_002494"
     CDS             6881..7507
                     /locus_tag="ACG827_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0484592.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACG827_002495"
     CDS             7530..8234
                     /locus_tag="ACG827_002495"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0484593.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACG827_002496"
     CDS             8426..9259
                     /locus_tag="ACG827_002496"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0484594.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2833 a         1792 c         1656 g         3022 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 ttt
//
LOCUS       DAZKOU010000104         8683 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000104 DAZKOU010000000
VERSION     DAZKOU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.105"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(281..2662)
                     /locus_tag="ACG827_002497"
     CDS             complement(281..2662)
                     /locus_tag="ACG827_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0484595.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2652..3299)
                     /gene="recU"
                     /locus_tag="ACG827_002498"
     CDS             complement(2652..3299)
                     /gene="recU"
                     /locus_tag="ACG827_002498"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0484596.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3363..3902
                     /locus_tag="ACG827_002499"
     CDS             3363..3902
                     /locus_tag="ACG827_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0484597.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3992..4411
                     /gene="gpsB"
                     /locus_tag="ACG827_002500"
     CDS             3992..4411
                     /gene="gpsB"
                     /locus_tag="ACG827_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0484598.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4433..4800
                     /gene="rnpB"
                     /locus_tag="ACG827_002501"
     ncRNA           4433..4800
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG827_002501"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5009..6175
                     /locus_tag="ACG827_002502"
     CDS             5009..6175
                     /locus_tag="ACG827_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0484599.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6221..7717
                     /locus_tag="ACG827_002503"
     CDS             6221..7717
                     /locus_tag="ACG827_002503"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0484600.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7798..8481
                     /locus_tag="ACG827_002504"
     CDS             7798..8481
                     /locus_tag="ACG827_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0484601.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8490..>8683
                     /locus_tag="ACG827_002505"
     CDS             8490..>8683
                     /locus_tag="ACG827_002505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484602.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2733 a         1572 c         1844 g         2534 t
ORIGIN      
        1 ttgataataa caatgggatt attaagaagt ggcactaaat aaaagactag tgtgtattta
       61 gccgatttaa ttataaacaa gtaaatggat aaagtatatg taatgataaa agaaaaatgg
      121 attttctaat ttttaagcta aatgttagat cacacatgta tatgctggat agtcttataa
      181 aaaatcatgt aatacagctt ggtacatgat ttttatttgt agctgttttt tacatagaca
      241 caaaaaagaa accacgcttg tagacgtggt ttctttttta ctatctgttt ccagaagctg
      301 aggtgctgct ggatggtgta tggtcatttg cagcatccgc tccgctagtt gctggctcac
      361 tggaagaagc aggaggagca gatgaagacg gtgtcgagct ttcttcggat gaagaagaag
      421 gaaccgttgt tgaagacgct ggctcgcttg tttctgctgt cgtgctttct tttgaggact
      481 cagcagttga ttcgctttga ctagtagagc tagagctttg agtcgttgtc tctgttgttg
      541 agcttccagg agtctggaca tagctggaag atggaatagt tgttgatggt gtaattgcat
      601 tcgatctagc tgtatattga tctttgtagt acagttcacc gcccacacga attaatccgc
      661 ttggcatctc ccagtctgta tttgtcacgt tagctgaaac atactgcata agctcgcgat
      721 aaacatcaga ggctacgtgt gacgattctg aagtgacagg cgtcattttt ttattgtaac
      781 ctgtccacac ggaaatcgaa taatttggcg tatagcccgc aaataggata tctggataca
      841 ccccagaaga aataccaagt tttgcatact cgtcatctgt gtaattggat gttccggtct
      901 taccggcttg atataaccca gcgatttgcg cattcgtacc agttccttca gtgatcgtat
      961 cttttaaaat gtctgtgatc atataagctg tttctggaga catggctgtt tttccatctg
     1021 gctcataagt ttcttctgtt ccgtcttgga agacgatttt attaacatat tgaggtttgt
     1081 aataagtccc tccattggcg aaagccgcat aggctgctgc catttttaag gaagaagctc
     1141 cgtattttgt cccatcttgt tcttccgtat tactagagat cgcattcgat tgatggatcg
     1201 tactgtattc gatcccaaga ttttttaaga aagaagccac tttatctgaa ccgacttcat
     1261 tgaataattt gactgctgga acattacggg ataagtaaag tgcttggcgt aaagtaatgg
     1321 tgcccatata ttgattatcc cagtttccta caggagtcga agtcccttca tagttatacg
     1381 gtgcgtcagt aatagttttt ccagtagaat atttcaaata ttcaaaagct ggtccgtaat
     1441 ctgtgacagg tttcatcgta gaaccaaaat cacgggaagt attcactgct aagttgttgc
     1501 ctaaagttac gtcttctgcg atgtgacgtc cgccaatctg ggcttttact ttcccggtat
     1561 tggtgtcgat aagggtggag gcaacttgca tctcgtcgtc tgggtaagag acatactgat
     1621 ccgtgttgac gatatcatat agtttctttt gggcgtccag atccaaattc gtataaatct
     1681 ctaaaccgtc tgtgtagaca ttcttatctg ttttttcttg tacttcattg atgacttctt
     1741 tgacataatt atcgacgatc ttagtattgt catcggattg agttagttct tgcagaccat
     1801 ccgttaccgg tacattaaca gcttgatcat attctgtttg agagattttt tcgttttgaa
     1861 gcatcgtata caaaacagta tctcgtcttt tcttcgcctg atctggataa acataaggat
     1921 cataggcgga aggggcttga ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt
     1981 ctgataattt cttaccaaag tacatctcag aagctgtctc catcccgtac aagccgtttg
     2041 acatatacac tttatttacg tagtaggtca agatctcttg tttcgatttt ttttgttcca
     2101 atcttacagc catccacgct tcttgggcct tgcgtttcag agtctgatcc tcggcactgg
     2161 tggagaagaa ggaaagtttg atcaattgct gtgtcaaagt acttccgcct tgcagtccgc
     2221 cagaggtaaa gttagagagg gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt
     2281 tataaaaacg gcgatcttct accgacacga tcgcatcttc taatgttttc ggtaattcat
     2341 tcgcagaaat tttttcacgc ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg
     2401 tatatagttt ggaagatacg gtagcgtcta attttttatc cgtcaattcg ggcgcatctt
     2461 ttgcgtaata ccagaaaagt cctacacctg ctaaaaagag gatacagaaa aatgagagta
     2521 ttcctaaaag gatcttgata aataatcctt ttttatgtgt tccagaggac tttccagaac
     2581 ctttgccaga attcttcttc acactctttt taggagtagg ctgtttctgt cgtctagagc
     2641 ttctagtttg ttcatttgcc attcttgctt tctccttcaa tagataatgt tgataaatag
     2701 aagtcaaccg cgtccaaata aggaatccgc ggagaaatac cagttttgat ctctattcct
     2761 agttcttcga tcaaagataa aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag
     2821 taagcaatca aatgaacact attcaaataa aaacagcgct tcaaagacga aaaccataac
     2881 aagacaaagc agaccccatt ttgttcgagg catgctttca tatgttcgat ttgatgggca
     2941 tggaagtttt taaagggaaa agatgttttg ttcttggttt cttttgcttc aaagtccaag
     3001 taaaagcctc gatagacgcc attatagtct gtagttgagg cttgggtaaa gtaggcttcc
     3061 ttgatcacag cagcacttct cttaggatag tctactttca cgatttggat aggggtaggc
     3121 tttttatgga taaccgccaa cccgcgggaa agataatatt tattactttc attgattgct
     3181 tcttcaaagc gcatcccccg attgccgaat tcagtgatct gatttctttt ttcttgaatt
     3241 ttttcgacgg gagaggcatt cttataataa ggttggccat ttggatagcg caataccatc
     3301 tttaatcaca ctcctaggcc acattatagc aaaaaagcgg atagaaagaa aaggaatctg
     3361 agatggataa gtcaaaagta atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt
     3421 ttaaagaaaa tgatcctaaa atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc
     3481 agttagcaga agaagggctg gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat
     3541 gggctgtgca aatagtagcg gaattgaaag aagaatatcc ggaactatct cttggtattt
     3601 tgtatccttt ttctgatttt ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat
     3661 tagcagaaca attagcagat tatgtcgaag ctgttagcca tcaaccctac caatcaccta
     3721 gtcagctaaa gaatcacact cgttttatac ttgagcatac agtgggctgt ctgctgatct
     3781 atgatgaaga attccctgga aaaacgaagt atttttttga agatgcacag aattaccaag
     3841 cagatcatcc ctacgaaatt cggctgatca gtatggacga tcttcaaaat tttagtgaat
     3901 aatcgatatt gtgtttgatt tatcaacttt tttcctatac aatgaaaaag gtagaaatgt
     3961 tattacgtaa atgaaaacta gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga
     4021 cattttacaa caagaattca aaacaaaaat gcgcggctat gatcccgttg aagttgatga
     4081 attcttagac aatatcatca aagattatga aacttacagc aaagaactcc ttgctttaca
     4141 agaagaaaac gatcgattga gtgcaaaagt agctcaatta tctaaaactc aaggagcagc
     4201 tcaaacacgc gtgcaacaaa cagaggcacc aaagagcgca gcagtgacca attttgatat
     4261 cttaaaacgc ttgtcaaatc ttgaacgtga agtatttggt aaaaagcttg atcaacaagc
     4321 aagtgcagtc aaaccagcac aacctaatcc aaataactac acaaatgcag acacaagctt
     4381 agatgataat gaaaaaacac gtcaatttta atttttgatt aatgaatttg cggttttcgg
     4441 gtaatcgcgg tctgacttga tcaggctgag gaaagtccat gctcgcacag gctgtgatgc
     4501 ctgtagtgtt cgtgcttagc gaaaccataa gctaaggtac tctttggagt aacggcagga
     4561 aaacaagcta aggcttcggc tatgcttaag tatccttgaa agtgccacag tgacgaagcg
     4621 gctggggaaa cccatccggt ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg
     4681 gtaggggcgc tttttgtctg gaagaagaac aggacaaaaa ggcagtataa ctgcagatag
     4741 atggttaccg ctttgttttt tctccctgga aaagcaaagt acagaacatg gcttatagaa
     4801 aaccgcaaac atcagggaat taggtcgtga caaaagtaat gcgttataag aaacaagaaa
     4861 aacatttgaa agctattttt caatacttca gatgaatcgt tcttattaca aaacaaactt
     4921 ttgtatcacc ggttattcgg aatttgagta aaagtgtggg gtaggactga tgtcccacct
     4981 ccttttttta taaataagga gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc
     5041 aactgcggca agcgggctag aagcactggt tggtaaagaa ttaagagatc ttggaatcga
     5101 atgccaagtt gagaacggac gtgcacgttt tgaaggaaca atggaaacga tcgctacagc
     5161 taatttatgg ttacgtacag cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta
     5221 tacgtttgat gaattgtttg aaaacgtaaa agcattgcct tgggaagatt ttcttccact
     5281 agatgcagct tttcctgtag ctggaaaatc gatcaagtcg aaactatata gcacacctga
     5341 ttgccaagcg atcacaaaaa aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc
     5401 agcttctgta ccactgacgg aaactggcgc acattttcag ttggaagtag ctttgctgaa
     5461 agaccatgtg atggtaactc tggatacgac aggaccaagt ttattcaaac gtggttatcg
     5521 cttagaaaaa ggtggagcac cattgaaaga aaacatggca gcagcactta ttatgttgac
     5581 aaattggcga aaagaccgtc cattttatga tcctgtatgt ggttcaggta cgctttgtat
     5641 tgaagcagcc ttaataggcc ataatatcgc accaggattc aatcgtgagt ttgcttgcga
     5701 atcttgggat tggttcagtc aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa
     5761 agcagattat gatattgaat tagatattac tggttcagat atcaatggac ggatgattga
     5821 aattgccaaa gccaatgcag aagaaatcgg attaggctct tcgattacct ttaagcaaca
     5881 agcagtcaaa gacttcaaaa cggataaaga atacggggtc atcgtggcta atccgcctta
     5941 cggagaacgt ctaggagaag aagaaactgt tcggaaatta tataaagaaa tgggcgatgt
     6001 attccgtcca ttaaaaacat ggagtaaata tatcttgact agcgacttag cgtttgagga
     6061 attttacgga caaaaagcaa ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga
     6121 tctattccag tattggggaa ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta
     6181 ctaattgatt cattcaattg gatgtttgga ggaataggga atgaaagaaa aagagttttt
     6241 acaagaagta aaagaaatca atcttctaca acaagcttta ggaatcctcg attgggatac
     6301 acaaacaggt atgcctgaaa aagctagtgc ataccgaagt gaagtagaca gctatcttta
     6361 cagcatttat ttttcaaaaa aaatcggtcc aaaaatcaaa gaagccattc gctatttttc
     6421 tgaacatccg gaagaattgt cggaagttgg aactgccgtt tttgccaaag taaaagaaga
     6481 gtatgatttg gaacatcgcg taccggaaga tcttatgaga aaatatacag ctgccgtttc
     6541 ttcagcacac ggacaatggc aaaaagctag agaaacacaa caattttctg attttcaaca
     6601 cgcattgaat caaaatattg aattaacaaa acagttgata ccttactgga gaaaagatga
     6661 aaagacgaat tatgatgttt tgctgaatca atacgaaccc gggatgactg tcgaaatcct
     6721 tgataaggta tttgatcagc ttaaagaagg aattctttcg atccgccgta cgctccaaga
     6781 aaaagggact gaacctgaaa cagacttttt aaatcggaga atgacgaaaa aacaacagaa
     6841 gagatttgta gtgaaagtaa tcgaacaact tggttatgat tttagtcgtg gtcgtttaga
     6901 tgacactgtc cacccattta tgactggaat taatccgaat gatgcacgaa tcacgacacg
     6961 atggaacgaa acagatttca aaatggctgt ttttggagtg atccacgagg ctggtcatgg
     7021 tatgtatgaa caagacatcg atgataaata tgcgtataca ccggtttatg aaggtacatc
     7081 aatgggtatc catgaatcac aatcgttgtt taacgagatc attattggaa gtagtcgcag
     7141 cttctggaaa aagcaatatc ctttctttca agaatgtgca gaaggaacat tcgatgatat
     7201 tccttttgac aaattttatc gttcattgaa ccatacaaaa tcaagtttgg ttcgtatcga
     7261 ggcagacagt ttgacttacc ctctccatat catcattcgt tatgaaatcg aaaaaatgat
     7321 tttcaatgaa ggggtagatg ttgctgaact acctgaaata tggaatcaaa aatatgaaga
     7381 gtatctggga atacgaccaa caaatgatgt ggaaggcatc ctacaagacg ttcattggtc
     7441 aggtgcaagc tttggttatt ttccctctta tgcattaggg tacatgtatg cggcacaatt
     7501 acttcattct ttgcagcaag aaatagatgt ggaaaatgtt ctagcgtcag acgattattc
     7561 tccaatcaaa aactggatgt cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa
     7621 tcaattaatc atggatgcaa cacatgaacc gcttaatccg caatacctga tcgactatat
     7681 gaaagacctg tattttaatg tatatcaggt aaaataaaat acaatttaca aagatagctg
     7741 aaattcatct aatctataga tgttttcagc tatttactta agaaagaagt ggaacgaatg
     7801 gataaattac aagtgatcgc tgattatagt atggaccaat tagcgcatga tcaaacagga
     7861 catgggagcg atcataccaa aagagttgtc aagttagctg aaaaaatttt ggagacggaa
     7921 ccgcaggcag accgctttgt gacgcttgca gcagcttacc tacatgatac gattgatgat
     7981 aaagtcgtta aaaatgagaa cgaggcaaag caacagcttc gtgtattcct tcgctctttg
     8041 ccaatagtag aggaacagat ctcgatgatt ttttccatta tcgaaaatat gtccttttct
     8101 aaaaatctct ctgaagcggc cgaactatct ctggaaggta agatcgttca agacgctgat
     8161 cgtatcgaag cattaggagc aatcggcatt ttgcgtactg cttatttcgg cggtggacat
     8221 gggcacccga tttttgatcc tgaactgtat ccgcaaacat tcaaggacaa agaaaattac
     8281 cgaaaaggca cgacagtgat caatcatttt tatgaaaaac tgttcttgct gtctgataaa
     8341 atgaatactg attacggata tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc
     8401 ttagaagaat tttttctgga atggtctatt gacgatgaaa attttactcc agacaattgg
     8461 atcaaaaata acaaaaaatg atggaggttg tgacagaagt ggtcagctcc aagatatttc
     8521 ggatcatttt ccgtaaaagt tacttctgga acactgttta ccaagaggtc gtgagcctgc
     8581 cgttcagctc cgagtcacaa ggaacaattt caaaaaatag cttctcgtgt ttgatgaata
     8641 ttgtaacttg tgattcggaa ttggcaggag aacaccgttt att
//
LOCUS       DAZKOU010000105         8494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000105 DAZKOU010000000
VERSION     DAZKOU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.106"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG827_002506"
     CDS             complement(<1..149)
                     /locus_tag="ACG827_002506"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0484603.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG827_002507"
     CDS             complement(593..1714)
                     /locus_tag="ACG827_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0484604.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG827_002508"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG827_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0484605.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG827_002509"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG827_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFQ0484606.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG827_002510"
     CDS             complement(4304..5215)
                     /locus_tag="ACG827_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0484607.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG827_002511"
     CDS             complement(5232..6239)
                     /locus_tag="ACG827_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0484608.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG827_002512"
     CDS             complement(6236..8365)
                     /locus_tag="ACG827_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0484609.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG827_002513"
     CDS             complement(8370..8480)
                     /locus_tag="ACG827_002513"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484610.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZKOU010000106         8023 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000106 DAZKOU010000000
VERSION     DAZKOU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.107"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..1797
                     /locus_tag="ACG827_002514"
     CDS             259..1797
                     /locus_tag="ACG827_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0484611.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1794..2759
                     /locus_tag="ACG827_002515"
     CDS             1794..2759
                     /locus_tag="ACG827_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484612.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2746..4068
                     /locus_tag="ACG827_002516"
     CDS             2746..4068
                     /locus_tag="ACG827_002516"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0484613.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4131..4235
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4309..5052
                     /locus_tag="ACG827_002517"
     CDS             4309..5052
                     /locus_tag="ACG827_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0484614.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5067..6059
                     /locus_tag="ACG827_002518"
     CDS             5067..6059
                     /locus_tag="ACG827_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0484615.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6071..6808
                     /locus_tag="ACG827_002519"
     CDS             6071..6808
                     /locus_tag="ACG827_002519"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484616.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6798..7520
                     /locus_tag="ACG827_002520"
     CDS             6798..7520
                     /locus_tag="ACG827_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0484617.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7604..>8023)
                     /locus_tag="ACG827_002521"
     CDS             complement(7604..>8023)
                     /locus_tag="ACG827_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0484618.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2570 a         1389 c         1751 g         2313 t
ORIGIN      
        1 aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag
       61 atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct
      121 tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt
      181 aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat
      241 aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag
      301 ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca
      361 ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta
      421 ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc
      481 ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt
      541 ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca
      601 gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca
      661 ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact
      721 ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga
      781 aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc
      841 agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca
      901 gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa
      961 accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa
     1021 gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg
     1081 agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt
     1141 actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa
     1201 gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta
     1261 aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa
     1321 catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc
     1381 acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg
     1441 aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga
     1501 gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg
     1561 actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa
     1621 gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata
     1681 acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca
     1741 gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta
     1801 aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc
     1861 taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa
     1921 aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact
     1981 tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg
     2041 tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc
     2101 ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa
     2161 aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag
     2221 aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg
     2281 acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt
     2341 ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa
     2401 tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac
     2461 cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga
     2521 aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa
     2581 taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc
     2641 ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga
     2701 caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat
     2761 gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca
     2821 ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga
     2881 tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg
     2941 agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa
     3001 cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc
     3061 gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa
     3121 gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg
     3181 gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa
     3241 gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct
     3301 gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg
     3361 tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt
     3421 gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg
     3481 gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta
     3541 gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg
     3601 caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa
     3661 ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca
     3721 agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct
     3781 gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt
     3841 accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt
     3901 gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc
     3961 gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc
     4021 atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa
     4081 ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc
     4141 tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag
     4201 ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc
     4261 atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat
     4321 ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt
     4381 tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca
     4441 gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg
     4501 gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta
     4561 gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg
     4621 ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg
     4681 ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag
     4741 caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa
     4801 ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc
     4861 agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata
     4921 cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta
     4981 ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt
     5041 tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt
     5101 tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt
     5161 tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc
     5221 tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag
     5281 gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt
     5341 atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg
     5401 ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt aacatccatt
     5461 caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg
     5521 tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa
     5581 gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag
     5641 gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag
     5701 aattagatga acggttagat tactatactc cggtaattat tacgaatgat caactgatca
     5761 aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag
     5821 ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc
     5881 ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga
     5941 attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg
     6001 gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg
     6061 agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat
     6121 attagaagac atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag
     6181 cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg
     6241 aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc
     6301 gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa
     6361 aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt
     6421 aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc
     6481 cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac catttggagc
     6541 gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc
     6601 aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag
     6661 gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa
     6721 acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt
     6781 attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt
     6841 tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag
     6901 aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc
     6961 atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa
     7021 ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa
     7081 aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc
     7141 acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt
     7201 tgatccattg gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt
     7261 tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc
     7321 caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg
     7381 ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg
     7441 agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt
     7501 tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg
     7561 ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg
     7621 cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg
     7681 ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt
     7741 aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga
     7801 tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag
     7861 caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa
     7921 caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt
     7981 gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAZKOU010000107         7811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000107 DAZKOU010000000
VERSION     DAZKOU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.109"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..641)
                     /locus_tag="ACG827_002522"
     CDS             complement(<1..641)
                     /locus_tag="ACG827_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0484619.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="ACG827_002523"
     CDS             complement(643..1407)
                     /locus_tag="ACG827_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0484620.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="ACG827_002524"
     CDS             complement(1415..2092)
                     /locus_tag="ACG827_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0484621.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="ACG827_002525"
     CDS             complement(2129..3514)
                     /locus_tag="ACG827_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0484622.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="ACG827_002526"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG827_002526"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG827_002527"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG827_002527"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0484623.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="ACG827_002528"
     CDS             4453..5310
                     /locus_tag="ACG827_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484624.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="ACG827_002529"
     CDS             complement(5391..5726)
                     /locus_tag="ACG827_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484625.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="ACG827_002530"
     CDS             complement(5793..7163)
                     /locus_tag="ACG827_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0484626.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="ACG827_002531"
     CDS             complement(7241..7777)
                     /locus_tag="ACG827_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0484627.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2219 a         1665 c         1268 g         2659 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa t
//
LOCUS       DAZKOU010000108         7788 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000108 DAZKOU010000000
VERSION     DAZKOU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.110"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..209)
                     /locus_tag="ACG827_002532"
     CDS             complement(<1..209)
                     /locus_tag="ACG827_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFQ0484628.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINL"
     gene            complement(223..1473)
                     /locus_tag="ACG827_002533"
     CDS             complement(223..1473)
                     /locus_tag="ACG827_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0484629.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(1835..3301)
                     /locus_tag="ACG827_002534"
     CDS             complement(1835..3301)
                     /locus_tag="ACG827_002534"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0484630.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGDQCRTKVL"
     gene            complement(3622..4386)
                     /locus_tag="ACG827_002535"
     CDS             complement(3622..4386)
                     /locus_tag="ACG827_002535"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0484631.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(4414..5112)
                     /locus_tag="ACG827_002536"
     CDS             complement(4414..5112)
                     /locus_tag="ACG827_002536"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0484632.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5124..5903)
                     /locus_tag="ACG827_002537"
     CDS             complement(5124..5903)
                     /locus_tag="ACG827_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0484633.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(5919..6863)
                     /locus_tag="ACG827_002538"
     CDS             complement(5919..6863)
                     /locus_tag="ACG827_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0484634.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6909..7688)
                     /locus_tag="ACG827_002539"
     CDS             complement(6909..7688)
                     /locus_tag="ACG827_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484635.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2186 a         1641 c         1273 g         2688 t
ORIGIN      
        1 aataaattaa taagtatagt acacaaaaaa aacttttctt ttctatttac ttgtctcatg
       61 tagtacgttt tttcgccttt tgactcagct gaatattctg tctgttctgt tataacaaaa
      121 ctctcatact tctccatcag tggttttaac atcattaact gttcataatg ccccccagag
      181 gaagccgcaa aacatatctt tttgttcata ttaacctctc tatcagtcac gctgaaaaat
      241 atctctagtg taaacttttt ctttaatatc atccaagcag gtatcatatc gattggcaat
      301 aatagaatca cacatcgcct taaactgctc taaatcgttg ataaccttac ttccgaaaaa
      361 agtctctcca tctttcagtg caggctcata aatcaccaca gtcgcacctt tagcttttac
      421 gcgcttcata actccctgga ttgaactctg acgaaaatta tcactgttgc tcttcatggt
      481 cagccggtat ataccgatta aaactggatt ttccctgttg acatcatact gattatattc
      541 agcgtagttg taatagcccg ccatctgaag aacacgatct gcgatgaaat ccttacgggt
      601 acgattagat tcaacgatag cctcaatcaa atttccagga acatctgcat agtttgccaa
      661 caactgcttg gtatcttttg gcaagcaata acctccatat ccaaaggatg gattgttgta
      721 gtgagttcca atacgaggat cgagacaaac accattgatt atctgctgag tattcagccc
      781 cttcatttca gcataagtgt ctaattcatt gaaatagctg actcgcagtg ctaaataggt
      841 atttgcaaac agctttactg cttcggcttc agtgaatccc ataaaaagag tatccacgtt
      901 ctccttaatt gcacttttct gcagaagtgt tgcaaaaaca tgagcagcat ttaacaagcg
      961 ctcatcagtc atatcagtac caacaataat acgagatgga tacagattat catataaagc
     1021 cttgctctcg cgcagaaact ctggactaaa aataatgttc ttactccccg ttgtctcacg
     1081 gactgattca gtgtagccaa caggaattgt agacttgatg accatgatgg tatcaggatt
     1141 cacgtccatc acaacctcaa taactgactc aacagcagaa gtatcgaaat gctgagtacg
     1201 actgtcatag ttagtcggtg tagcaataac aacgaaatct gcggatttat aagcgctcct
     1261 tgcatccaaa gtagcagtta agtcaagttc tttttctgta agatactttt caatatattc
     1321 atcctgaatt ggagacttcc gtttattaat cagttcaatc ttttcaggga taatgtctac
     1381 cgcggttacc tggtgatgct gcgccaatag tgtggcgata ctcaaaccaa cataaccggt
     1441 gccggctact gcgatgttgt actttttctt cacaatatct cctctttcct ctcttaagta
     1501 tggagaatgt tactcctacg gctattcatc atgtcaaaat aacacagctt agaatgttga
     1561 taaaataatt ttccagaatg tgataaaatc tctttcattt ataaaccaca catctaattt
     1621 tatctattct ccacaagaca tgtagagtat tctttaataa caagatacga ccttactaaa
     1681 tacttcaaaa aattatcata tttaattcat aaatataaat cttcatttta aatgtatgcc
     1741 caaaaattta tctgtaatgc gtcatcaaaa tctagattcc aaatctttat caatttttat
     1801 ccactcattc taccacttta gtaatatttt ctatttataa cactttcgtc ctacattgat
     1861 cccccctctg aagtacagtt tttactgtct taaataaaat tttaaaatct aaccccatac
     1921 tccaattttt tatatattcc gtgtcaagtt ttgctacttc ttcaaaatca gtaatatcac
     1981 ttctaccact tacctgccac attccagtaa ttcctggctt aatagcaatt cttgcacgat
     2041 gatgataatc atattcttca tactctgatt taaggcatgg tcgaaatcct ataaggctca
     2101 tctttcctgt tagaacgttg aaaaattgag ggaattcatc taaagacgtg gatctaataa
     2161 attgaccgat tcctgtcttt tttgttccat ctggaagtat tttatttccg ataattcttg
     2221 gatcaaactc catcttaaac ataagattat tgctcatctt attttctttc attaattcta
     2281 cttttctttc ttctgcatcc atatacatac ttctaaactt atacatcttg aacggtttac
     2341 cattctgacc tatacgttct tgtgcaaaga aaattgggcc tggtgaatta atataaataa
     2401 gtggagcaat aaagataaaa ataattcccg ttgcaataca tccaacaata cctgcaagaa
     2461 tatcaattgt tcgttttata aatgcctgtt gagttgtcat aacattgata cttgttgtaa
     2521 gtactgtata ttctcctata cgaccaatag attgttttcc caaagcacta tctgatactt
     2581 ttgcaagatt caaatgtaca gtaaggccca tttcagagca tcgttcaata aattctttca
     2641 tacacgaagt acttttattc aaaataataa agacttcatc tacccatccc tgacataagt
     2701 aatccgctgc atttttggaa tcagaaacaa ctgtcactcc ctcaacatct tcaccaatca
     2761 tgcttttatc tatgataatg acaccattta ttttatacat tttatagtta tgctctttca
     2821 cattacgaat aactacttca gcaatactac tagatgtaac tataataagt gatttgttcc
     2881 cctcattttt cattttatgt ttcaagtaat gtttccataa gaggcgagtt atatatgtaa
     2941 ttacaacgta taaacctccc atcaagaaaa gaacgactct agaataatca tttccaccct
     3001 gaacactaat gagatacaaa cttcctaaca gcaccaacat aatcgcttgc tttacagaag
     3061 aagcaaattc tatatagtac cctcttttta atacacctct atatgcttca aacatgaaaa
     3121 taactaaaag atctgccagt tcaatgaaga ctgccatgtt ttgatacatt ggaatcacat
     3181 aaggattcca atctacctgg cgtaagacat acgatagata aaaagcaagc tggatacaca
     3241 ataaatctaa aataataaaa tcaatatgtt taacccatcc ccttgaactt ttcttgtaca
     3301 ttccttttta ctcccctctt tttaataaaa taaattgtat tacttgtttt ctctcctaaa
     3361 tatctttctg aaggattatt gactcgacaa attggcttgt ggatattctt atatatagaa
     3421 catccgtaag ccaacttaaa actccttaac aaaatttttt tactcatatt gtagatcaaa
     3481 acaagtatcc aagaaatcat attttatttc ttttttctgc ttactcaaca acagctaaaa
     3541 gaaaaatcat cataaacaaa ttcaactaag ccaacgatcc ccttgtgaaa tcaagttttt
     3601 tcttgattta cgaataaaga gctaaaataa accaaacttc ttctttttta cctcttcata
     3661 gactggataa ctgatttcat ccccgttgat cagatccttt gcgacttgtt tcatctgtac
     3721 tactttttcc ttaccaaagt cctttgcaat ctgctcatag cactctttta agtaaaaggt
     3781 gcgcttagag accccatggg catcagaagc agccatctgc acgagattat gtttgaccat
     3841 cgttttggct gttttctgga tgtctttgcc aaatacccca acataacttg gcgcagtgag
     3901 ttgggccaaa catcccatat ctaggaacgg aagcaaatga tttgggtctt cgcgaaaatg
     3961 cacgttgcgt tctggatgga cgattactgg tgtctttcct agttctaaca actcaaaaaa
     4021 aagccgttct gcatataacg gcacttccag tgtcggaaat tcgatcagaa gatatgtatc
     4081 atctaaatct gtaaataata tttcatctcg gtggatgtct tcgatgagcg ttccggtaat
     4141 acgtacttct tgtccttcca ataacgtcaa aggcaactgt cttttctcta attcggcttg
     4201 taacgaagcg actagagaaa tgacttgtga tttttcattg ctgtattttc cattattgtg
     4261 gtgtggtgtg cataaaatat gcgtgatgcc ttgctgaatc gctttttcag ccattgcaat
     4321 acttgcttct aagttttctg ccccgtcatc gattcctggt aggatatggc aatgtagatc
     4381 gatcatttct cttgtctcct ctcttcttat tccttaattt ccgtagtaat aaccagcatc
     4441 tttgctatgt tctgctccgt tgtacacaac acctaagact cgtgcttgta ccatctccag
     4501 cagttctttt gctttagtca aggattcttt acgtgacata ttttcacgta cgaccaataa
     4561 agtgccatcc gtttttaaag acatgatctg tgcatctgtt acggctacta ctggtggcat
     4621 atcgaatatc accacgtcaa ataactgtct tgcttcggat aggatctgat ccattcttgg
     4681 cgagcttaac aattctgatg ggttgggtgg cttaggtcca ctaggcaaaa tcgacaagtt
     4741 ctcgactggc gttcgttgga tcacatctgc tacacttcct gatgaactaa gtgctgtact
     4801 taatccactg gcattgttca gctggaatgt cttatagacg acgggtttac gtaaatctgc
     4861 atcgactagt agaactcgtt gacctgattt agcaaaaacg actgcaatgt ttgctgcagt
     4921 ggtggatttc ccttctccag gacctgatga agtcacgaca atcgttttga tttgctgacc
     4981 agctgcggag gcaaactgga tattcgtccg gatcgtacga tattgttcgg cgattggtga
     5041 agatgggttt gttaatgtga tcaaactgac tgcatgagtt tgtgctgctt tttgtttctg
     5101 tgttcgtgcc atttttcttc ctcctagact cttgagcggc ttctgcgtga tggggctgtt
     5161 ttatcgtttg tagcttttgg tcgtgcagga gctgttcctt ttttacggat accagcgttc
     5221 aattctttcg ctgacatttc tggaactgtt cctaagattg ggaatcctaa tgtatctgtc
     5281 acatattttt catctttcac tgtccgatcg agtaattcca ataagaatgc cagacctaca
     5341 cctaccatca ttcctaacat taagccagtc attaaattca atttattatt tggcgaaaca
     5401 ggtgtcgtat tggcaactgc acctgaaatg atcgagattt tatcaacatt catcacatct
     5461 tttgcgttct cttggaaaac ttgtgaagtt gtgttagcga tctgttgagc agccactgca
     5521 ttcgtactta ttgcctgaat cgagaacatt tgtgaatttt ctgcctgatt aactgagatg
     5581 gcatctttga tttgttcagc agtcattttc acattgtctt ctgtttctaa acgatctttg
     5641 acttcattga gcactaaatc actgacgatc atatctttat acgtattgat catttgcagg
     5701 ttcgtattga catcattgac atttgcagtg tctgattgtg gcagtgtcac gatcaattgt
     5761 gcttgtgaac tgtattttgg tgtcaagatg aaaaatgtta cgattcctga gatggccaag
     5821 ccagcaaaca ttgagatgat gattattacc aagtgtttgc gtaatgtttc aaaaatttct
     5881 ttcaagctga ttgtctcttc cattttttct ccactttcct atttattctc tagattcatt
     5941 tgatctttta attctttttt cacacgatca agttcttgat cactgacttt ttgataggag
     6001 atattatctt gcatgaagcc ttctccttgc agctgatctt gcttaatcgt accaaatgcc
     6061 tcccgataat ctaaagcaat ttttttcatt tcatcaaagt tcatgtctgt tttcatattc
     6121 gactctagag cattcaaaac ggattgatag ttgctgacac cgtttagact caatactttt
     6181 ttcgctacgc cttctacgat ctttctttgg cgcccttgtc taccgtaatc accatttggg
     6241 tcttcataac gcatacgaga ataagataat gcctgatccc cgttcaatgt aattttaccg
     6301 attggaaagt catagccatc ttgtgagaaa gtgaggtcat tattgacttc gataccacca
     6361 actgcatcaa cgagttcctt tagtcctttc atattgatag agacatagtg atcgattggg
     6421 atgtctacat atttttgtac ggtatccatc gccatggcag ctccgccgaa tgcgtaagcg
     6481 tggttgatct tgtcttttgt gtcacgacct acgatgtcga catacgtatc tcgaggaatg
     6541 ctgacgatcg ttgtctgttt gtcttcagga ctgactgtgg caaccatcat cgtatctgat
     6601 cttccttgat cgacccgacc taaatctcca gtatcaatcc ccatcaatag aacagagaaa
     6661 gattcttgtt tgtccagatc aactggtttc gttcgttctc cttgggttcg ttccactgat
     6721 tcgtaagttt tctcaataga atgtgagaga tccagatata attttgctcc tactcctaca
     6781 attgctaata ctaatacgat aagaactgct aaaataatca aaaatatttt tttccctctt
     6841 gattttcttc ttcgtcttgc catagtttcc tccgtttgct aaccttgctt gaatcaacgt
     6901 tttaactgtc aatagcttat attgctatag cccatttgct gagcaacagt ttgtgctttt
     6961 gttgcgtatg cggctcttat tttttcattc cggatctgct ctatttgact gagcgccgtc
     7021 aaatattgat cgtaagtagc ggtagctgtt acttgaccgt ctttcagcat cgtatcgaat
     7081 agctgatcta gttgcgtgac ttgtgcaatt tgggtatcag cgaatcctag atattgcata
     7141 acgactgttt tccaagcact atccttaaaa aagctcatat tttcacgtac atgggcgaca
     7201 tctgcacttg ccaacttttc tttgatgatg acatcatcca cagcttgctg ccagtttgat
     7261 acgtttttag taaataattt attgacagcc gtctggatct tcagttttga ttctgcatcc
     7321 tctaactgcg ttaagactgc tttcttttct tcttggatcg cagcagcttc tttaggtaat
     7381 tctgattcct tgatgtcata atcatctgcc gaaagtttga tagaagatag tttatgtgac
     7441 aaatttgaaa ctgtttcttc tgttatttca ggatttagga aaattttttc ttttgtactg
     7501 tacagcttat ctacttcttt tgataaagag gtcacggtgt ctttttgatt ggtgatggca
     7561 gattctgcgt aattgatccg ttgatgatat tgccgctgat tattgagatg gtatccaaac
     7621 aagcaaatca caatagcgcc tgtgcctacc gccacagtag ccaataactt gacgaacggc
     7681 ttttccatag attcactcct aaatattgat gatagataag taaagatgtg attcattcac
     7741 atcagaatga gcgcttttta ttttaaaagc ataattaaac taatatat
//
LOCUS       DAZKOU010000109         7600 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000109 DAZKOU010000000
VERSION     DAZKOU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.111"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..427)
                     /locus_tag="ACG827_002540"
     CDS             complement(137..427)
                     /locus_tag="ACG827_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484636.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="ACG827_002541"
     CDS             complement(545..805)
                     /locus_tag="ACG827_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484637.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="ACG827_002542"
     CDS             complement(942..1355)
                     /locus_tag="ACG827_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0484638.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="ACG827_002543"
     CDS             complement(1811..2296)
                     /locus_tag="ACG827_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484639.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="ACG827_002544"
     CDS             complement(2435..2650)
                     /locus_tag="ACG827_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0484640.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="ACG827_002545"
     CDS             2874..3674
                     /locus_tag="ACG827_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0484641.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="ACG827_002546"
     CDS             3693..5561
                     /locus_tag="ACG827_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0484642.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="ACG827_002547"
     CDS             5554..6045
                     /locus_tag="ACG827_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0484643.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /locus_tag="ACG827_002548"
     CDS             6033..6587
                     /locus_tag="ACG827_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0484644.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..7600
                     /locus_tag="ACG827_002549"
     CDS             6605..7600
                     /locus_tag="ACG827_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0484645.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctga
//
LOCUS       DAZKOU010000110         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000110 DAZKOU010000000
VERSION     DAZKOU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.112"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACG827_002550"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACG827_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0484646.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACG827_002551"
     CDS             1483..1935
                     /locus_tag="ACG827_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484647.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACG827_002552"
     CDS             1949..2638
                     /locus_tag="ACG827_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484648.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACG827_002553"
     CDS             2763..5114
                     /locus_tag="ACG827_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0484649.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACG827_002554"
     CDS             5194..5589
                     /locus_tag="ACG827_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484650.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACG827_002555"
     CDS             5914..6531
                     /locus_tag="ACG827_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0484651.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACG827_002556"
     CDS             6572..6871
                     /locus_tag="ACG827_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0484652.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACG827_002557"
     CDS             6905..7072
                     /locus_tag="ACG827_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0484653.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACG827_002558"
     CDS             complement(7117..>7537)
                     /locus_tag="ACG827_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0484654.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAZKOU010000111         7486 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000111 DAZKOU010000000
VERSION     DAZKOU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.113"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACG827_002559"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACG827_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0484655.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="ACG827_002560"
     CDS             complement(2944..3378)
                     /locus_tag="ACG827_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484656.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="ACG827_002561"
     CDS             complement(3395..4600)
                     /locus_tag="ACG827_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0484657.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="ACG827_002562"
     CDS             5294..5680
                     /locus_tag="ACG827_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0484658.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="ACG827_002563"
     CDS             5774..5965
                     /locus_tag="ACG827_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484659.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6479..6811
                     /locus_tag="ACG827_002564"
     CDS             6479..6811
                     /locus_tag="ACG827_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0484660.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6721..6915
                     /locus_tag="ACG827_002565"
     CDS             6721..6915
                     /locus_tag="ACG827_002565"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0484661.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            6954..7100
                     /locus_tag="ACG827_002566"
     CDS             6954..7100
                     /locus_tag="ACG827_002566"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484662.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            7107..7262
                     /locus_tag="ACG827_002567"
     CDS             7107..7262
                     /locus_tag="ACG827_002567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFQ0484663.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         2481 a         1320 c         1082 g         2603 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7081 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7141 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7201 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7261 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7321 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7381 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7441 ttgccaatac tttttctttt ttctaataaa cgcaaacaga cacctt
//
LOCUS       DAZKOU010000112         6896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000112 DAZKOU010000000
VERSION     DAZKOU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.115"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG827_002568"
     CDS             complement(<1..182)
                     /locus_tag="ACG827_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0484664.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG827_002569"
     CDS             517..951
                     /locus_tag="ACG827_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0484665.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG827_002570"
     CDS             974..1294
                     /locus_tag="ACG827_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484666.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG827_002571"
     CDS             1435..1626
                     /locus_tag="ACG827_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484667.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG827_002572"
     CDS             complement(1697..1954)
                     /locus_tag="ACG827_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0484668.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG827_002573"
     CDS             2112..2375
                     /locus_tag="ACG827_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484669.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG827_002574"
     CDS             2399..2587
                     /locus_tag="ACG827_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484670.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG827_002575"
     CDS             complement(2670..2855)
                     /locus_tag="ACG827_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484671.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG827_002576"
     CDS             3070..3441
                     /locus_tag="ACG827_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0484672.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG827_002577"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG827_002577"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG827_002578"
     CDS             complement(3709..4137)
                     /locus_tag="ACG827_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484673.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG827_002579"
     CDS             complement(4197..4955)
                     /locus_tag="ACG827_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0484674.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACG827_002580"
     CDS             5250..5447
                     /locus_tag="ACG827_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484675.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACG827_002581"
     CDS             5553..6035
                     /locus_tag="ACG827_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484676.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACG827_002582"
     CDS             complement(6093..6689)
                     /locus_tag="ACG827_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0484677.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2333 a         1059 c         1355 g         2149 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttga
//
LOCUS       DAZKOU010000113         6862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000113 DAZKOU010000000
VERSION     DAZKOU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.116"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            527..721
                     /locus_tag="ACG827_002583"
     CDS             527..721
                     /locus_tag="ACG827_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484678.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            912..1161
                     /locus_tag="ACG827_002584"
                     /pseudo
     CDS             912..1161
                     /locus_tag="ACG827_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1405..2235)
                     /locus_tag="ACG827_002585"
     CDS             complement(1405..2235)
                     /locus_tag="ACG827_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0484679.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2443..3777
                     /locus_tag="ACG827_002586"
     CDS             2443..3777
                     /locus_tag="ACG827_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0484680.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3818..4729
                     /locus_tag="ACG827_002587"
     CDS             3818..4729
                     /locus_tag="ACG827_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0484681.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4791..5579
                     /locus_tag="ACG827_002588"
     CDS             4791..5579
                     /locus_tag="ACG827_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0484682.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            5576..6192
                     /locus_tag="ACG827_002589"
                     /pseudo
     CDS             5576..6192
                     /locus_tag="ACG827_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            6194..6712
                     /locus_tag="ACG827_002590"
     CDS             6194..6712
                     /locus_tag="ACG827_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0484683.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2065 a         1186 c         1372 g         2239 t
ORIGIN      
        1 ttattttata ataaagatat gatgactaat ttagctcaac tattccagcg tcagctgaag
       61 atattgcaat tcccgattcc ttaatcattt atctggatga gacagaaagt gaagttgcaa
      121 cagatgtgca tgtagttgag gataaaaatg atagtttaaa aattaatagt tcagatactt
      181 tactaagagt aagtcctatc ttttcagctg ttatttagat gcaagtagca actaagttag
      241 gtagtactcc tgcaatggat aggtatttgt gtgttcgata tataagtggt cgaagtatca
      301 aggttatgta tactggatgg gagatagaag agttaaaaat tggaggtcac cgccacaaat
      361 ggaatttaga tttagaggta ccttagattt ggtatagtta ttaaacacaa gatggcctgg
      421 tttgtaggga aagtcgaaga gtatttttcc acttctgcgc ccctttaatt tttctagtaa
      481 tgccgtgaat ttgtgattaa ttggaaatat gaagaaagga gagtctatga aaacaacact
      541 agttctattc tataaaaagc atccttattt tacacttctg ataaatatat tgttggctag
      601 tgttatcggg atttctgttg aatatctaat aaataaagat ttcataggtt cttgttttta
      661 tacggcttta tttcttggac ttcttgaagc attttccatt tataaaaaat caaaaaaata
      721 aaatagattg ccgtaagaga tctttttcat ttcatttttt tctgaaaacc cgttgtttct
      781 gtcagtgaag cagcgggttt ttagatttat tgtctttaga cctaattgag cgcgccgagc
      841 gattgaaata caaaaccact tgctatgcgg gtggagcaat aaagggaaat agtttagccc
      901 gtacaaagta gatgtgtaaa taccacattg tattcacacc aaagtataga agaaaaataa
      961 tttataatca atatcgagcc aatattcagg agatcatcaa gagattgtgc aaatctaaaa
     1021 tataaatttg ggtatcgaca cttttgggtg gaaggatact atgtgacaaa agtaggacaa
     1081 aatgaagcga ccatcaaaaa gtacattcaa gaacaagaaa aacatgatag tgcgttagat
     1141 aagctgagtg tacgagagta agacgatcct tttaagggct aaggagtagt acataaacct
     1201 tttaagaggt gggcaaaact ggcaaaggca atatggcttg aacataaaat atgaaagcca
     1261 gcgtctttag gcgctggccg gtgatttgag cttatagctc tagagaaaac cacccttttg
     1321 aagggtggtt atgattaatg actttagtca gttttgctca caaagcgtgt ggaacaaaaa
     1381 acgggtgttt ctacgggtag atatttattt cttgaatgga ggtgtcccat acatttttgg
     1441 tgttactggt tctaattttg gtgcagcgcc ttcgatctta ggttctttca accattgata
     1501 ttcgccttcg ccatccagtg ctttgccact agcccaagaa ccttcactac tttggtctcc
     1561 ctctgagaag ttatagaagt cataagcaat gtcttttcgt tctttgtcac gcgggaaagt
     1621 acttgggact aagattgaac cttctttttc ttctaattct ttcactgcac attcccatag
     1681 attttgatga tagctatctc ttgcaaggag aacagacagc atatctttta cgccacgatc
     1741 atcgatcatt tcgtaaatac gtgctacttg aagtcttcct tgtgattcgg catttaaatt
     1801 agctctaaag tctgctaaca agttgccact cgcaatgata taggcaccgc tccatggatt
     1861 gcccacgctg tcagcaggac gaggtcctaa gccagctacg atcgcgtgct gtggattcat
     1921 tccagagata atggcaccga ttgttggatc tttttgcatc gctgcttctt gatcttcaac
     1981 aggcgcaccc tctaataaac gagcaaccat tgtcgccaac atttccacgt ggccgatttc
     2041 ttctgttgca atgtccatca gcatatcttt atatttttct tctcctcggc agctccagcc
     2101 ttggaacagg tactgcatag ctacactcat ctcgccatat tggccgccga tcagctcctg
     2161 cagatatttc gcgattaatg gattcggacg atctggtttt gcttcaaact gcaattcttt
     2221 ctggtgtcta aacatcaaaa ttcctcctaa tctattttta atacattctc attctgctac
     2281 gaaagattaa aaaattcaaa gaaagcgctc gcgaattata taaagtattg gtatcattaa
     2341 taattttttt agaatcatac cttataaatt gttattttca gaacaaaatg atatcgttat
     2401 attaaaaccg gcatcggtta cattaaaatt tggaggtaaa atatgaaaaa attattgttt
     2461 acagggttgc tggcaagttc agcattgttt ttattaggag gatgcggatc aagtaatgca
     2521 agcgatgcca gttcaagtgc agcaaattct tctgaaaaaa caagtgatgg aagaaaaaat
     2581 attgatttct ggtccttttg gggatccggt gcacgaaaag aagctatcga agaaatcatt
     2641 gaggatttca atcaatcaca aaacgagatc catgtaaatt ataagtatca gccatggggt
     2701 gacatctgga ctaaatcttt atctgcaata acagctggaa atgcacccga tgttattgta
     2761 caggacataa attccgttgc ccaaagagca gaagcacagc aagcaactaa tctctccaaa
     2821 tatgtagaaa aagacacttc taaggatttt tatccgcagc tttgggatac agtagaatat
     2881 gaaggtgatc cttatgcgat cccattcaat acagatacac aagtcatttt ttataataaa
     2941 aagatattta aagaagctgg aatcagtgaa caagatctac cgacaacttg ggatgaatta
     3001 gaagaaacag cacgtaaact agataaaaaa gaaggaaaag aatttacacg catcggtttt
     3061 tatccattat ggaatttagg ggctgatgta tgggcgctaa acgcagataa cgggactagt
     3121 tggtttgaca aagatgaaaa agtcaagatt gatactaaaa ataaagtgga agccttggag
     3181 tggatccaag actggcaaga ttattatggg aaaaatacga tcaataaatt agaagccgaa
     3241 tttgggtctg gtgtatctga tccatttatt tccggattag tagccatgcg tgcccaaaat
     3301 atcaattatt actctagttt gatggaaaat gcaccggatg attttgattt tggcgtgatg
     3361 cagctcccag aaaaagaaga aggttcaggc cattggtcgt ggggtggcgg attcgtcttg
     3421 gaagtaccat acggagcaaa agatcctgag gcctcttatg aattcatcaa atatctttct
     3481 tctccagaag tacaagaaaa attcggcgaa aaaagttttg atatcatggc gagcaagaca
     3541 gccaatgaga atttagtgaa taatgacaac ctaacggata aaggaaaaat gatctatcaa
     3601 atggcggacg aaaactttaa aaatacggtg atcacaccag ttcctttaga tgctccggat
     3661 tacacgacat tagtgaatga acagattgac cagattcttt taggaacaaa atcaccaaaa
     3721 gaaggattgg cagatgcaca aaaagcagta gaaaacttag tcaagcaaaa tcaataatcc
     3781 atttttgact gtctaggtac agaggaggca agaaaatttg aagcgtttat ctatccgaaa
     3841 gaaaaaagaa gcaagatggg cctatttatt tatctcgcct tttattatcg ggttcttggt
     3901 atttatgcta gggcctatgc ttttttcagt tattggtagt ttcaccgatt ataatttgac
     3961 atccaagatg aatttcattg gtctagcaaa tttcaaaaga atgttcgttc acgatgattt
     4021 attttggaaa tcgttataca acaccgttta tttcgtcatc ttcaatgtac cgctgacgac
     4081 gattttcagt gtgtttttag ccacagtact caatcaaaaa atcaaaggaa tcagtgtatt
     4141 ccgtacagct ttctatttac cggcagtctt atcaggagta gcagtctaca tcttatggat
     4201 gcagctgctt tctccatctg caggattgat caacacattt ctgggctggt tccatatccc
     4261 tggaccagct tggctgtttg atccacaatg gacgaaacct gcgttgattt tgatgaaaat
     4321 atggagttct ggaggagcaa tgctacttta cttagctaca ttacaaaatg ttcctagatc
     4381 tttatatgaa tcagcagaaa tcgatggagc aggcgtttgg ggaaagttca aaagtattac
     4441 tttgccatta atcactccta ttatttttta cgatgtgatc acctctacga tcggttcttt
     4501 ccaaattttt caagaagctt atgtcatgac gaaaaatgga acaggcggac ctgcaaattc
     4561 tttgcttttt tataatctgc atatgtggaa caaggcgttc gtagcttttg atatgggtta
     4621 tgcgatggct atgtcaatga tcctgtttct gatcgtgttg gttctgacgt ttgtcaattt
     4681 gaagcttgca cctaagtggg tctattattc tggaggtgac ggcaaatgat ggattatttc
     4741 aaaaagacca gtgtgaatcg gcaaacaaaa aaatactaag ttatgtcctc atgacagtaa
     4801 taggaatcat tttgatcatt ccattgcttt ggatggtgtt tacttcattg aaaccaatgg
     4861 aagaaatcgt tcgttatcct ccgacctttt tcccagaaaa aatcgtatgg gaaaactatc
     4921 tggatacgat tgccgccttt cctttttggc gctatgcaag aaatacatta ttcatcactg
     4981 tgctagtagt gatcggtaat gtcctttcaa attcttttat tgcttatgga tttgcgaagc
     5041 ttgattttcc tgggaaaaag ctgatgtttg ctttggttct ttctacgatg atgatcccgg
     5101 ggtttgtaac gatgatccct caatatgtat tattctccaa gatcggctgg gtaggtactt
     5161 atttgcccct gatcgttcca tcgttcttcg gcaatgcgtt caatatattt ctgatgcgtc
     5221 aattttactt atcgatcaat aatgaattga tcgaagctgc tgaaatcgac ggagcgaatc
     5281 atttgtatat ttggagtcat ttgatgcttc cattgacgaa acctgccttg attaccattg
     5341 cgatcaattc cttcaacgca gcttggaatg atttcttagg tcctctgtta tatatccaag
     5401 atcaagaaaa atacacgtta cagattggct tgcaagtttt tcagaaccaa gcaacgacac
     5461 aatggaatta tctgatggca ggagcaacac ttgtcttgat cccgacgatt ttgctgttct
     5521 tttttgcaca acgttatttc attgaaggga tggatttgac aggaggttca aaaggatgat
     5581 gaatcgatcg attggtaaaa aagccaatga ttatttctta gaaatcattg aatggattcc
     5641 acgtcttatc catttacagc tactatggtt cctgtgcgtc ttgcctgttt tcacattagg
     5701 aactgcaagt cgaacagttc tgtatatgat ttatcattac aaaaaaatga agagaaaaag
     5761 ctcggttcac tattctggaa aaagtttcgt gagaacttct ctctttatgg aaagcaagat
     5821 agtttagcaa gtgtttattt tcttcttcta ttgatcgatt atcagatttt ccgctattgg
     5881 ggtgggggaa ttggctatac attgatgtat actacgttgt ttttgcttct attaagcgcg
     5941 cttctcttta gttacaaaat cttgcttcaa ctggaacaaa aagaagcttc ttggattact
     6001 gcatttttct tgttttttaa tgatttcaaa agcgcgcttt tctatttggc aggcacgttg
     6061 atccttcttg tcgcattctg gtttgcagga ccaatctatt ttgctttatt aggtttcagt
     6121 ttcttgtttt attttcaagt cctgcttttt atccatcgta cgcaagaaaa ggttcttaaa
     6181 aaggaggaat aacatgttgc gtttgattgc taaaatcgat gatagtatta tcaacaagtt
     6241 cgccaagcaa cggaaaacgg atagtcgaaa aatcaatttt ttgaaattta tcagtaatat
     6301 ctctaatggt ggttttattt ggttgctcct tgctgctgtt ttagtcttga tacctgatac
     6361 tagagaatat ggtctagcca ttcttatcgg cagtttgatc tatttttttg ttggtttgct
     6421 ttttatcaaa aaaacgacaa gacggaaaag accttatgaa aaaaatcgtc ggcttgtcct
     6481 ttacacaagt aaaccgaaga cttcttcttt tccttcagga catacagcgc tagccttttt
     6541 atcagtagct gcgttgttcg aattaagtcc agttgcagga atattagcct ttttatttgc
     6601 ttgtttgatc gcttattcga ggattttttt acttttacat tatcctactg acattttagg
     6661 aggagctatt ttaggaagtg ttattggcat cctatgcgct gccgttatct gaattaattg
     6721 tacgaataaa gcatgagtgc gtaaatgcgc atttgtgctt tatttttttg tttcgaggaa
     6781 agcgtagttg tttattcatt tgtcttaata acttaagttt tgttaaatga gaacgttata
     6841 aaattttata atttaatttt aa
//
LOCUS       DAZKOU010000114         6741 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000114 DAZKOU010000000
VERSION     DAZKOU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.117"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..909
                     /locus_tag="ACG827_002591"
     CDS             19..909
                     /locus_tag="ACG827_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0484684.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(982..1806)
                     /gene="nadE"
                     /locus_tag="ACG827_002592"
     CDS             complement(982..1806)
                     /gene="nadE"
                     /locus_tag="ACG827_002592"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0484685.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1818..3290)
                     /locus_tag="ACG827_002593"
     CDS             complement(1818..3290)
                     /locus_tag="ACG827_002593"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0484686.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3927..4289)
                     /locus_tag="ACG827_002594"
     CDS             complement(3927..4289)
                     /locus_tag="ACG827_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0484687.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4512..5264
                     /locus_tag="ACG827_002595"
     CDS             4512..5264
                     /locus_tag="ACG827_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0484688.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5404..6327)
                     /locus_tag="ACG827_002596"
     CDS             complement(5404..6327)
                     /locus_tag="ACG827_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0484689.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6342..6614)
                     /locus_tag="ACG827_002597"
     CDS             complement(6342..6614)
                     /locus_tag="ACG827_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484690.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2055 a         1347 c         1155 g         2184 t
ORIGIN      
        1 ttttaagagg tgatatatat gcaactacaa ataaacgaag aatccttacc ggtatatgaa
       61 gcattagcta gtaaaacaag gatcaagatc atccagctac tttccaaaaa aaagatgaat
      121 gtcaaagatt tggcaaaaga acttggagtg agcagtgcta tcacgacgat gcacgtgaaa
      181 aagctagagg aagcaaacat catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc
      241 cttcgtgtcg ataaaatcga tattagtttt ccagaaaaaa tattcaatgc ttttgatacg
      301 aaagaaacat cgatacctat tggtcactat acaaattatg caattgaacc aacatgcggt
      361 ttagctacga tccatgattt tatcgggaaa gtggatgaac cacgctattt tatggatcct
      421 cgaaggatgg atgcccgcat tctctggttt accagcggat ttgtcgagta tcaggcacct
      481 aattttttaa atacagagga tacattggag atgttggaag tttctgtgga aatttcttct
      541 gaatttcctt tttcaaatga caattggcct tcagatatca ctttcagcct aaatggtgtg
      601 gagctaggaa catggacgag ccctggagat tttgctgata tccgcggtaa atatacgcct
      661 gattggtatc cagataatct taatcaatac ggcttgctga agacgatccg aattaccaaa
      721 cacttgacga atatgaatgg agaacccttg tcaaacatca cgatcaacga catccctaaa
      781 gagcaagaca cttggcattt gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc
      841 tgtactttat ttggtaaagg attcggtaat tacgatcagg atatcaaatt aaaagtttac
      901 tacagctgat atttttcttt tctattttat aaaacaaaaa cagccaatcc ctatgaaggg
      961 acggctgttt ttgctttatt gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg
     1021 tcgtttatgt tgtgtcttgt tccaccatgc ttcgattttt tcttgtgctt gagcgggaac
     1081 ttcttttcct tctagataat catcaatttc attatatgtg acacctagtg cgacttcatc
     1141 tgcaatcatt ggtttcccat cttcaagatc tgcagtcgga atttttgtgt ataatttttc
     1201 cggtgcattc aatgcttgaa gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg
     1261 caaaatgtcg gctccaccat caccaaattt cgtgaagaat ccagtaatgt tttctgcagc
     1321 atgatctgtc cctaatacgg ctcctgctct ttctccagct acggcatatt gcgtaatcat
     1381 acgttgacgt gctttgatat ttcctttgtt aaaatcgctg attcccacac ctgcttcgct
     1441 taatacttgt acttgtgcat caacagctgg tttgatattt actcgcagac taacatcggg
     1501 ctggataaag tcaagagcag cttttgcatc ttcttcatct gcttgttcac cataaggaag
     1561 acgaacagcg ataaattgat agttttcatc ttttgtttct tcacgcattt cttccatcgt
     1621 tagttgcgcc aaacggcctg ctaaagtaga atcttggccg ccgctgatac ctagtacaaa
     1681 agttttaaga aacggatatt ttttcaaata tgctttcata aagtcaatac ttttacgtat
     1741 ttcttctttt ggatcaattg ttggtttgac acctaattca ttgataattt ctgtctgcag
     1801 gctcatttat tctcctccta tgcttctgta tgaagttctg ctacgctttt tttcattcgt
     1861 tccatgatag ccattttatg gttccagcat tcagtagaca gatcgactgg gtatttttgc
     1921 ggattcaagt ctcgtttata ctcttcccat agtgaatcta gattttcttt tgtatattct
     1981 ttgatttcgt ttagtgcagg taattcgtaa actctttttc cttcgataaa gatatcttgt
     2041 aaaactgggc gagctgtaaa gtctcgaaca gttttattga taaacgtatg cactggatgg
     2101 aacatgaaga tagcttcttc ttctcgaggg tcttcatccc ataatgtcac gtagtctcct
     2161 tcagatttcc catcaaaatt tcgagtgatg cgccacactt gttttttccc tggagtagtt
     2221 actttttctg cattgcttga taacttgatc gtatccatca tttttccttc atcatcttcg
     2281 atcgatacta gcttgaatac tgcaccaagg gctggctgat cataggctgt gatcaatttt
     2341 gtcccaacac cccatacatc aattttagct ttttgcatct ttaaattcaa aatcgttgct
     2401 tcatccaagt cattcgaagc atagattttt gcttccgtaa atccggctgc atctaattgt
     2461 tcccgaacac gcttagatat ataggccata tctccactgt cgatgcgtac gccttggaag
     2521 ttgattttgt cacctaattc tttcgccacg cgaatcgcac ttggcacacc tgatttcaac
     2581 gtatcataag tgtcgactaa gaacacgcag tctttatggg ttttagcgta ggccataaat
     2641 gcttcataat catttccata agattggact aacgaatgag catgggtccc gctagctggt
     2701 atcccaaata tcttcccagc acgtacattg cttgttgcat ctgctccgcc gatatatgcc
     2761 gctctagttc cccagactgc tgcatccagt tcttgtgccc gtctagttcc aaattccaat
     2821 agtggatctt ctccgatgac agatttgata cgagctgctt ttgtcgcgat caacgtttgg
     2881 aaattgacca tattcaacag tgctgtttct actaattggc agtgcgctaa aggcccttcc
     2941 acttggatca atggttcatt tgcaaaaact agttctcctt cacgagcaga acgaactgtt
     3001 gctttaaatt caaaattttg taaatactcc aaaaaacctt caggatagac ttctaatcca
     3061 cgcaagtaag caatatcgga atcattgaac gtcagattct ctaaataatt gactaaccgt
     3121 tccaatcctg caaaaaccgc gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca
     3181 aaaacagcat gtaaatcagc tcgacctaat tcccagtatg tctgcatcat attgatttga
     3241 tatagatccg tatgcagcgt caaactgtcg tctgggtaag tattattcat cttgtattac
     3301 tcctcttggc aagttttcat ttcctaccca ttataacaaa aaaaattaat aggaaaagaa
     3361 atcttccatt ttttctgaaa aagcacatgt tttacggata tttcttgcta aaaatgaact
     3421 ccatctgcta aaactatcct ttcttattgg tatttaacct aaacgagaaa gaatacttat
     3481 tttattcaat gtatccgaga cacgaagaaa ttagcgtttt ctatttttct tacttgataa
     3541 aataagtaga ttttcctact acaatcgtag ccgttaagat accgatgatt gaacaaatat
     3601 aaccaaatgt ctctacaaca taatcaatgg caaaagtttt tgaagcaata atacgtcttt
     3661 ccttttttgc ttcttatccc caaagaaacg gatataactt attatgtatt gaattttttt
     3721 ggaaaggcga attatgacaa gatatacctt gcgttgaaaa aactcgcgga aaaaatatca
     3781 cacgttttta aaaagataca aagtaagaaa gcagctgtca atagaatgga aacaaaaatg
     3841 gcgagacatc aatcagtctc gccttgtttt ttattgtata cattagatat ggagcaggtt
     3901 accataaaga tgattattag tcgttattaa atagaatcct tcgtattttt ttgttctatc
     3961 actacaatat ctttatttgg acgtttgaat tgtattccgg cacgttctag ctcctgtttc
     4021 actcctacat ggactttgac atcttctcgt gtcattttta aaacgtgatg aatcgcatag
     4081 tcgatttctt gccttgtgat ttttttcttc gtattgatca tcacgacttg ctttttatca
     4141 tatgtgtcta ccaatagcag ttgtgttcga tctaacgaaa ataatttgta ataattaata
     4201 gccgtattcc caaaaaagaa ttccactact ttaggataaa atgtcttaat atttaaatta
     4261 ctattcacag gtgtttcgat tagcttcatc tcattcctct tttctatata gtcgatttag
     4321 atccaatctt aagttatgtt accatctttc ctagcattaa ggaatcggtt acaattgaac
     4381 ctttaaagac aaaaggattt accttttatc acttaatagt ataacataat ttcatgagaa
     4441 aaaaatacaa ttttttcttg accatctcgt tacgtaaagg attatgctta catttgtcag
     4501 gaggaaaaat aatggagtac acaataaaga aaatgtcaga gatatcagga gtcagtccga
     4561 gaaccttgcg cttttatgat gagattggac tgctaaaacc agctaggatc aattcttcag
     4621 gttatcggat ttatggcaaa aaagaagtcg atcgtctgca acacatctta ttttatcgaa
     4681 caatggcatt caagttggat gatatccaag aggtcttaga caatccatct tttgaccacc
     4741 agaaagcttt aatcaagcat cagcaaatgt tgctggaaaa acgggcacaa atcgataccc
     4801 tcttgacaac tgtccaacag acacttgata tgtacgaagg aggaagaaaa atgagtgaca
     4861 aagaaaaatt tgaagggttt aaacaacaaa agttgaaaga aaatgaagaa tcctatggac
     4921 aagagattcg acaaaaatac ggaaaaaaaa aggttacagc atccaatgaa aaatggatga
     4981 atatggataa agaaacgtat gaagaaatgc aaactgttga aaagcagatg ttatttgatc
     5041 tcactcttta cttaaaagaa ccagaagatg aaagcttagc agaccgtatc ttccaagctc
     5101 ataagaagtg gttatcttac agctggcctg aatatcagcc aaaagcgcat aaaggactag
     5161 gaatgatgta cgaagccgat gaacggttca ctgcatatta tgacgagcga agtggtcatg
     5221 gtgcagcaaa agctttaaat gaaattatcc aaaaaaaggc gtaatctttt acatgatcca
     5281 acaaaaaagt acacgtaaaa tgacctaata acaaaacagc agacacccaa gttagatttt
     5341 tatgctaact tttggtgtct gctccattct ttcacaagtt ttttcatcct actttaaata
     5401 tattcatcgt tgaataaacg tataaggtat tttcacttta tccttttttt ctgtaagaaa
     5461 caattgaaat gccatttctc cgcaagcttc cagatggtgg tcgatggttg ggaaaccaag
     5521 aagctcgctg atcagcaaat tatcgcgtcc aatcaccact ggtgtcttat cttgcagata
     5581 gctttgcaag atgcctgctg caaccatatc cgaatttgta aagacaccat caactccaag
     5641 ctctttaaaa aagaagcctg cttgttttcc tttctctgca tctatacaat cccaaaaaac
     5701 aagtgattca tcaaaagatg gaaaaagttg atgacatagc tgcaaagtga tttttgagtt
     5761 ataacttagc ctgccacttc gccccaacgt gatgcccaat tgctttatac cttgctgctt
     5821 caaataagta agaccttctg taattgattg ttctcgatca ataaagacac agcttgcctg
     5881 ttttcccttg atttcttcac aaaaaactag aggtccatat ttttgatatg ccaataaatc
     5941 atctagtgta ttggcacgag acgtaatgat caatccatcg aaagctttag ctgcaaattc
     6001 ttcaagatac cttcgctctt tttctttctg atagcccgtc ggtaaaagtg tcactttgta
     6061 atcataatca aacgctgctt caatgatccc actgatcaac tgatcaaaat acggatgatt
     6121 ggtataaggc agaatcactc caatgctttt cgtttttcca taacttaact gtctggcatt
     6181 tgcattaggg atataatcga cttctttcat gaccgcaagt atttctttcc ttttttcctc
     6241 atctacatat ggatgtccat tgattacacg tgaaatagta gaaactgaat aacctgtgag
     6301 ctttgcgata tctcgaatgt ttgccactgc tgcttcgcca cctatacaaa aagattttct
     6361 tgtaaatagt ctaccacagc tggaccgcat tcaaaaaact gatacatttt tccttcatgc
     6421 gaaaaactga tccgctgccc gccccaatat atatcttttg tcaaattttc gatttcattc
     6481 attggtattt cttttactat attcctattc aacccttgaa aactgtacaa cgttaatacg
     6541 gatcccttga actcgacaaa cgcataaaaa taattgttcg ttgtgccatc tgttatctct
     6601 acataagtat tcattgcaag ccctcctttt gatactttga gtatagtgta tgaaacgcgt
     6661 ctcatagcaa ggagtttgtt ttgccaaaga aaagttttat tcctctttca ttattaccaa
     6721 agtctcttat tttaaattga a
//
LOCUS       DAZKOU010000115         6243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000115 DAZKOU010000000
VERSION     DAZKOU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.118"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..462)
                     /locus_tag="ACG827_002598"
     CDS             complement(136..462)
                     /locus_tag="ACG827_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484691.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(508..870)
                     /locus_tag="ACG827_002599"
     CDS             complement(508..870)
                     /locus_tag="ACG827_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0484692.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(907..1121)
                     /locus_tag="ACG827_002600"
                     /pseudo
     CDS             complement(907..1121)
                     /locus_tag="ACG827_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1249..1584
                     /locus_tag="ACG827_002601"
     CDS             1249..1584
                     /locus_tag="ACG827_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0484693.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1660..3657)
                     /locus_tag="ACG827_002602"
     CDS             complement(1660..3657)
                     /locus_tag="ACG827_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484694.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(3755..4030)
                     /locus_tag="ACG827_002603"
     CDS             complement(3755..4030)
                     /locus_tag="ACG827_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484695.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            4262..4996
                     /locus_tag="ACG827_002604"
     CDS             4262..4996
                     /locus_tag="ACG827_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0484696.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5035..6177
                     /locus_tag="ACG827_002605"
     CDS             5035..6177
                     /locus_tag="ACG827_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0484697.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2125 a         1144 c          897 g         2077 t
ORIGIN      
        1 aaaattcccg acaatcattg taatattttt ttgtcacctg tattagtatt taatttatag
       61 gaaactataa aaactcccta gtgaatttac acctttggga gttcctgttt ggacggtatg
      121 cataatacat ttaaatcaat ttaacattaa cacctcagca tatggtatct ccacatatat
      181 acggtctttc tttggatagt actcaatatt tatgttgata gcacttccat tgtctaatat
      241 agctttagcc cggtatgtgt tatctccatt ttcatgagta atcgtcacat catcacatga
      301 tcgatttaag ttgtactgtt cttgtaaaat ctgtgttacc aatggtttcg atgacttctc
      361 taatccagat gtacctgtca tttttgaaaa aatgatacct ataatagcca agacaacgat
      421 aactataatg ataaacttct tgttcatatt tactttattc aactttttat ctcctatctc
      481 caaaataatt tgctcttatc cctcttatca gtacattgac atccctaaaa gtacaccaaa
      541 gattgctcca gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttatctcg
      601 atataagtag cccataataa cagcaacagc accaaataca acaggaataa atgctaacgc
      661 tactaccata gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac
      721 gatttgttcc ttcatttcat taattgtctg ctcagtactt ttctttactg cgtctgctcc
      781 agttgtctca tctgtttctt gtagtgctcc gcaaaactgg caatacttac tttcatctgt
      841 tatttctttg ccacattttc tacaatacat gtcttctcct cctaaaaaca ttcaattgtt
      901 tattagttaa ctgtttactt tattaaatac atcagaaaac agctcattca ttggtacact
      961 taatatctca gaaagcatct tcatttgata gggtttagga atcgttctcc ctgtttgcca
     1021 cgaacttaca cattgctgtg taacattcat ttttttgcta actccgtttg agaaagtcct
     1081 ttttcttttc tgtatcggct gagattactt acttttttca tgattctccc ctcctgtttt
     1141 attatataca acaatatgat gtatttcaac tcataaaata taaaaaaact tcaaatacca
     1201 ttacaaatac aacaacttgt tgtactatac tgagatgggg gtgtattaat gaaacagaaa
     1261 caatatgcaa aggtcattag agaaaagcga aaagctctcg ggtggacaca aaaagaatta
     1321 gctgaaaaaa tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagag
     1381 cctaacctcg atagcttaac cgctctttct agagccctag gaacccctgt aagccatttt
     1441 ctagacaatg ttgtcgtaga ttatgaggaa gagtttttgg ctctctatcg ttctcttagc
     1501 actgaagatg cagttcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa
     1561 cgcaatcagc tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt
     1621 attgagatga aataagatta agagctatgg ataacctgtc tagtttaata caattgggtc
     1681 ttttctcttc cttactctcc gtccatcatc tcttgttaca cttgtaaatt gataaagagg
     1741 aactaattca actcctgata atatcaattc ttttattact tcatctatcc ctgctccttt
     1801 tgctaggtag actaaaaaac tactacttgc tttcacattg tatagtctct taatatcctt
     1861 agaagctcgt tcaagagtga atccttgttt aacaaagtag cctctgtttt cttttaaagc
     1921 attttccacg ctgttattcc caacattacg tagacgttca caaacagtgt tagtaagttc
     1981 tttctctgtc attagatatg ttccttgcca cgataagccc tgtaaaactt catgtgcttc
     2041 atctaaaatc aattgttttt ccttgctaag ttctgatcgg gaattttgag gaaatactct
     2101 tttagcctct ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt
     2161 aaaatgaaat ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt
     2221 tgcattaaaa acgtctaatc gatagacatt gcttctctta tgattaaagt ttcctaacgc
     2281 tttctcttcc tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc
     2341 taattttttt atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat
     2401 gtcataagtt ttaccatagt aaaaactcat ttttttagct aattgttccc ccgacaaaat
     2461 acaagtcaca ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata
     2521 gacacgtcgc atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga
     2581 ttcaagagtg tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata
     2641 caagtctagg ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat
     2701 tagcatcaca actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa
     2761 aatggaatgc ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa
     2821 cagacatctt aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct
     2881 ttcataaatc tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg
     2941 tttcatttct tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag
     3001 ttcatactct ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat
     3061 cggaagctca gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt
     3121 atatcctcca aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt
     3181 ctttttcaag tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa
     3241 tagaaagaca atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc
     3301 aaacgcagaa ttatgaacta aattgataga taaaaccttt tctatgctta agtatctact
     3361 atcatcttta ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc
     3421 cagaataatt actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt
     3481 gcttggaata aatgattgtg atgaatttag tatctctaca aactcatctc ttgtaacttc
     3541 tctatagtta tctaaacacc tatctcttac atcagttttt tctttttttg taactggctt
     3601 ctctaataat ttttctttat ctacataaat tggaatatta atcattgatg tttccatttg
     3661 tgtttcctcc ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc
     3721 tttattacta tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac
     3781 tatcaatatc agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt
     3841 attgagaaac atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct
     3901 gatagtccat atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca
     3961 ctgtaattat ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt
     4021 gtagagacat atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct
     4081 tcgtattttt tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat
     4141 gattaccagt tcatcgacat acacataata cctcgataaa aaagaggtgt caatatcttt
     4201 gtaaaattta tttacaacac ccctttttcg aggtatacta attttaggag gtgtggtcaa
     4261 tatgataaaa cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat
     4321 aaaagctata agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa
     4381 cggtcaatta attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat
     4441 accagagcga ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg
     4501 tggcaatgta gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga
     4561 tggtataaca tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt
     4621 acactctaat ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga
     4681 atttattgct aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat
     4741 cttaaaaata gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct
     4801 tttcctcact agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat
     4861 tcaattgaaa gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa
     4921 caagctagaa caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg
     4981 aggtgatacc atctagtggt aagtactcct tgtaaactaa gaggagatga tttcatggca
     5041 aaaatatcaa agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt
     5101 gccaccgatc cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa
     5161 caagaagctc aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag
     5221 aaagcacctg atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc
     5281 agtgttaaag cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca
     5341 gcatttggta agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat
     5401 gaatggcaca gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta
     5461 cgctacgggg ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca
     5521 cgaaaaaaag agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta
     5581 ctggatgctt ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca
     5641 tacacaggta tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt
     5701 aataaagaac tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt
     5761 caatctccga aaacaagagc ttctagacga gttatctctt tagatactga gactctttct
     5821 atcctcaaca actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact
     5881 agctctaaag agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact
     5941 gttaatgatt ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat
     6001 gggtttagac atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt
     6061 caacaacgat tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca
     6121 aataatgcac ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag
     6181 agctggtcaa aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta
     6241 tat
//
LOCUS       DAZKOU010000116         6202 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000116 DAZKOU010000000
VERSION     DAZKOU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.119"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1158
                     /locus_tag="ACG827_002606"
     CDS             <1..1158
                     /locus_tag="ACG827_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0484698.1"
                     /translation="LIPSIYNFGYLLITLIVIFPADFSQSEEEKKKKMVYFNNVFIII
                     IFLAALISIYQFIFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSIIL
                     SLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFR
                     KSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKT
                     PLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKV
                     DLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNY
                     AFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
     gene            1160..2230
                     /locus_tag="ACG827_002607"
     CDS             1160..2230
                     /locus_tag="ACG827_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFQ0484699.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            2217..3638
                     /locus_tag="ACG827_002608"
     CDS             2217..3638
                     /locus_tag="ACG827_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0484700.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            3651..4658
                     /locus_tag="ACG827_002609"
     CDS             3651..4658
                     /locus_tag="ACG827_002609"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFQ0484701.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            4670..5674
                     /gene="neuB"
                     /locus_tag="ACG827_002610"
     CDS             4670..5674
                     /gene="neuB"
                     /locus_tag="ACG827_002610"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFQ0484702.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            5671..>6202
                     /locus_tag="ACG827_002611"
     CDS             5671..>6202
                     /locus_tag="ACG827_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0484703.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRID"
BASE COUNT         2161 a          775 c         1081 g         2185 t
ORIGIN      
        1 ttaattccat ctatttataa ttttggatat ttgctgatta ctttgatagt aatttttcct
       61 gcagactttt cacaaagtga agaggaaaaa aagaaaaaaa tggtgtattt taataatgtt
      121 ttcattatca taattttttt agctgcgcta atatctatct atcaatttat ttttttgatt
      181 tcttatcatg tcccaacagg aacgccagga ttacttgcaa gacaaggttt tattgaacat
      241 cggttatttg gtgtctatac aagtccaaat gttggagcta tgtttggata tacttctatt
      301 attttaagtt taattagtgt attaatttca aaaataacaa aaacgattcg tgttttttat
      361 atgatgaatt ttatcgtaca aatattttat attacacttt cttcgtctag aggtgcccag
      421 gtagtaattt gttctttttt agtattattt cttttgttgt ttgccaattc aaaatttaga
      481 aagagcatta gagaagtact acctgtaaaa atcaaatgga tggtaagcat attattagtt
      541 ctattggtat atttttcttt tggtactaat tatgctaagg attttttagc aacaattcct
      601 gtatcgattg atagaaaagt agaagttata gagtcaaatg aatcagataa aactccactt
      661 ccagaaaaag aagtaatatt acagcattct agtgataact ccgaaatttc agctggtcgt
      721 tttacgattt ggaaagctgc gcttaaagtt ataagaccaa ctttgttatt tggttatgga
      781 gagaccgatt tctatgggaa tagcgatgca aaatttttga aaaaagtaga tcttgatact
      841 acagatgtga atgaactaaa aagagcacat ggaaacatgc ataatggatt tcttcaagta
      901 cttgtatcaa gtggtataat tgcttttata tgtatctatg ctttctatgt gttaaatatc
      961 tttagtttac tcaaaacatt ttttagaagt aaagtgaatt atgcgtttct tggtattgtt
     1021 ttaattttta tactatctat ttttatagat gaaatggtgg aggctcatgt acttttcaat
     1081 aagcgcgatg tcatttcaat tgttttctgg tattatttgg gaagcatttc ttacttgtat
     1141 ttaaaaaagg agaattaata tgaagacagc tttcattgct tggactcctt tacatttgat
     1201 taatatatta aatgtttatc aaaattttta ccgagaatca aaaggagatt tatttattta
     1261 ttctgaattt gaaaacgcta gaagatatag tgagcgtata aaagaattag ggctatttaa
     1321 tgatgttgtt tttgtgaatc accggaaaat cggttcttcc tttaaacgta aaattaactt
     1381 gttaactaac cataatcaat ttttctcgga tgatttatcc gtttatgatg aaatttttat
     1441 acagggggaa aattatttca caaaaatact tttctccaat gtttacaaaa aaaatccaga
     1501 tgtaaaacta aattatatag aagatggttt gggtacttat ttgaagaaga gtatattttc
     1561 aacaagtaaa aacgttcaaa gagtttttaa aatattaaat aaaaatagca tcttcaaaaa
     1621 aaaaattgac gattattttg tctattgtcc ggaattgtta ctttatgatg taaaaaatat
     1681 aaaaaaaatt cccaaaatag atgaaaacag taaattgatg aaaaacatac aatcaatctt
     1741 tgggatagaa gaagtttttg tcggagataa atgtatcttt tttgatcaac ctttattatc
     1801 tgatggttat ggcatagatg aaacagatat tttcgctaaa gtaaatagta tattttctgg
     1861 aaaaaaagaa actttaatta agcttcatcc tagagcaagg acaaatcgtt atggtgaaaa
     1921 aaacatattg aattccagtt taccgtttga agtcttgcta cttgaaaatg acttctcttc
     1981 aacacttttg attagtccta tatctacaat ttctttttca ccatctatga tgtttgaaaa
     2041 gacgatcaac tctattatgt tggctaaatt aattttaaat gaatatacag cattatctga
     2101 agataaaaaa gaaacattga caaatattgt tcttttctgt gaacattata accgtctatc
     2161 agaagaaaaa attttactcc cagaaaactg ggcagaatta agaaagttgg tagagtatgg
     2221 gattatttaa cacactttcc aagtcaaaat tagttaagtc cagtttctgg tatactatag
     2281 ggaatttttt tattaaggga atttcattca taactattcc tatttttgta aatattacta
     2341 ctcaagcaga atatggtttg attaataatt tcacttcatt tgtatcaatt ttttcgattc
     2401 ttgttgggct aagcctaaat tcggcaatta ataatgcaag ttttgactat aaagatgaca
     2461 taaatgaatt tatgtcatcg gtactttctc tagctacact ttcgttaatt gtgttcttat
     2521 taatagggaa catatatttt ttgttttcaa atgctttttt tgatatgaac caacttgtat
     2581 ttaatttgat gttattacaa agttttgcta gttttgtaat tagttatatc cagtcttatt
     2641 ttacgattaa ctttaaccat tttaaatatt tgattttatc ttttatatct acttttttga
     2701 atattgcaat ctctgtagct ttgatgcaga cattatattt tgatgataaa tatgttggca
     2761 gagtagtcgg cggaacactg tcatttgtgt tgataggttt agtaatttat ttaagaataa
     2821 tgataaaggg tgctcatttt tttgataaaa aacagtggag atacgcttta aaaatctctt
     2881 tgccgctaat cccacattcc ttagcaaata tagttttagc acaattcgat agaattatga
     2941 ttaattctta tttaggtcct gctgcggggg gaatatattc ctacattagt aacataggta
     3001 ttattctaag cgtaatatgg atgtctacta ataacgcctg ggttccatgg ttctatggtg
     3061 agatgagtaa aaataatgaa aaaaaagtga agaaagttag taactattat ttagtagtct
     3121 ttactttgat tacgatgatt gtaatggtag tttcgattga tttagcaaaa tttatggctc
     3181 cgaaagacta ttattctggg ttatctttag tggttccgat aaccttaggc tatttctttc
     3241 agtttttata tagtttacca gttaatgctg aattttatga gaaaaaaaca tcgtttattg
     3301 cgattggtac agtagcaagt gctatagtta atatactatt aaacatgatt ttcataccta
     3361 ggttaggaat aattgctgct ggatacacaa ccgtatttac gtatttttta ctttttattt
     3421 ttcattataa tatcgctaaa aaaattacga ataaacaatt atttgacaca aaaaaaatat
     3481 gtatcataat aatttttgta tcaatttttt ctttgatact tcatatttta atcaatcaaa
     3541 tgttgattcg ttacgttttg acatcattat tgataattag tttagttata tattttttaa
     3601 agattaagaa tcgaattata gatgactata attcatagga ggagatataa gtgtttaaag
     3661 gaaaaacaat attagtaact ggcggaactg gttccattgg aagtgaaatt gttcgtcagc
     3721 ttttaaaata tgaaccaaaa gctatcagaa tttatagtag aggtgaagaa aaacaatttt
     3781 atatgcaaca agagctctca aatcacacta atttgagatt tttaattggt gatatcagag
     3841 actctcaaag gattgattat gcatgtcgtg atgtggatat tatttttcat gctgccgcaa
     3901 tgaaacacgt tccagcaagt gaatataatc ctatggaagc tgtaaaaaca aatgtgatgg
     3961 gaactcaaaa tgtaatagat gcagcgatca aaaataatgt aaaacatttt gtaggtatca
     4021 gtacagataa agtagtgagt cctactaaca ccatgggtgc gacaaagtta ttgagtgaaa
     4081 aattaataat agctgccaat cagtataaag gaaaaacaga aacaatattt tcttgtgtaa
     4141 ggtttggaaa tgtcatgggg tcaagtggat ctgttattcc tctttttagt aaacaaataa
     4201 aaaacaataa agatattact ttgacagatg ttgatatgac acgatttatg atgtcaatac
     4261 cggatgctgc aagattagtt ctgtctgctg ctatgaattc aaaatttgga gaaacatatg
     4321 tcttaaaaat gccggcaatc aatattttaa ctttagctca atcattaata aaaatatata
     4381 atgatacaca tgatgaaaaa tatagaggtg aaataaaagt tgtaggtatc aggccaggag
     4441 aaaaattata tgaagagcta atgactacag acgaaattga acgctcatat gaaaatgata
     4501 aaatgtatat cattccacca cactatcatg ctactgacga actgtacaaa gattatgaaa
     4561 aaattaaaat cactgcatac tcttcagatg caacagaatt aatgagtgga caggaattta
     4621 ttagaatcgc aaaagagaat aatgtcgtga cttggtaagg agttttatga tgaaatcaga
     4681 agtatatgta attgcagaaa ttggtgtaaa ccataatgga agtctagatt tagctttaga
     4741 aagtattgat aaagctaaag aatgtggagc taacgcggtt aaatttcaaa catttaaaac
     4801 gaataagcta gtttccaaaa aggctccaat ggcaaaatat cagaaggata atcttaaaga
     4861 ggacagttct caatttgaaa tgctaaaaaa attagaatta tctcaatctg attttattag
     4921 aataaaagat tattgtatac aaaataaaat tgattttttg tctactcctt ttgatgaaga
     4981 aagtgcagac tttctaaaac aaattggtgt acaaggattt aaaattggct caggagattt
     5041 aacaaattta ccttttctta gaaaaataga tatgtatggg ttgccagttt tattatctac
     5101 aggtatggct gatattcatg aggtcagtga ggctgtagat tgtttcatag atagtccagt
     5161 tactatttta cattgtactt caaattatcc ggctagttct gaagatatta atttattggc
     5221 gattaacaca ttaaaagaaa tgttcgggat acccgttggg tactccgatc attcattggg
     5281 atatgatgtt ggtatttgtg ctgttgcgat gggtgctaag gtaattgaga aacattttac
     5341 cttggataag gaactacctg gtccagatca taaagcttct ttagatcctc aggaatttgc
     5401 agattttgta aagcatatta gaaacactga aatattttta ggtgatggtg ttaagcgagc
     5461 aatgccttct gaacttagta caaaagaagt atcaagaaaa agcctagtgg tcactgagaa
     5521 tttaaatgtg ggagatattc ttacagagaa atctcttaca ataaaacgtc ctggaaatgg
     5581 aatagctcct aagtttttat atcaatatat tggcaaaaaa gttaaaagac caataagtgc
     5641 agatagtgtt ctgcttgagg aagatgttga atgaaaaaaa tacttatagt aagtggaaca
     5701 cgagctgatt atggaatata taaacctgtt gctgagaatt tactacaaga taattgggat
     5761 gtatcatttg ttgtttctgg aatgcattta tcccataaat atggatttac taaagaattg
     5821 atcttagctg acggatttag aattagtgga gaagttcaaa gtttgttttt agagaacaat
     5881 aagggaaaca tggctcggtc tgtggcatta gagataaatg gtttcacaaa tatatttgaa
     5941 caaactagcc ctgattttgt attattacta ggggatagag gagaaatgtt ggcagctgcg
     6001 atagcttgtt tgtatcttgg aatcaaaaca gcacatattc acggaggcga agtgtctggt
     6061 actgtagatg aatcaataag acatgccata acgaaaatag catctgttca ttttgctgct
     6121 acggaaaaaa gcaaagagcg tttaataaaa atgggggaag attcttggag agtttttccc
     6181 gtcggagctc ctagaataga ta
//
LOCUS       DAZKOU010000117         6132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000117 DAZKOU010000000
VERSION     DAZKOU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.120"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..955
                     /locus_tag="ACG827_002612"
     CDS             110..955
                     /locus_tag="ACG827_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0484704.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1236..2456
                     /locus_tag="ACG827_002613"
     CDS             1236..2456
                     /locus_tag="ACG827_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0484705.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2453..5173
                     /locus_tag="ACG827_002614"
     CDS             2453..5173
                     /locus_tag="ACG827_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0484706.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5157..6101
                     /locus_tag="ACG827_002615"
     CDS             5157..6101
                     /locus_tag="ACG827_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0484707.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2293 a          958 c         1196 g         1685 t
ORIGIN      
        1 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
       61 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      121 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      181 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      241 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      301 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      361 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      421 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      481 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      541 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      601 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      661 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      721 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      781 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
      841 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
      901 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
      961 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1021 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1081 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1141 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1201 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1261 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1321 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1381 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1441 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1501 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1561 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1621 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1681 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1741 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1801 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     1861 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     1921 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     1981 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2041 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2101 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2161 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2221 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2281 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2341 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2401 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2461 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2521 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2581 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2641 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2701 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2761 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     2821 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     2881 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     2941 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3001 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3061 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3121 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3181 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3241 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3301 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3361 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3421 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3481 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3541 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3601 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3661 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3721 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3781 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     3841 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     3901 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     3961 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4021 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4081 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4141 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4201 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4261 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4321 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4381 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4441 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4501 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4561 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4621 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4681 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4741 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4801 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     4861 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     4921 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     4981 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5041 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5101 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5161 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5221 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5281 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5341 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5401 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5461 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5521 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5581 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5641 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5701 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5761 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     5821 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     5881 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     5941 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6001 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6061 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6121 ttcaaaacaa aa
//
LOCUS       DAZKOU010000118         6092 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000118 DAZKOU010000000
VERSION     DAZKOU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.121"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(256..543)
                     /locus_tag="ACG827_002616"
     CDS             complement(256..543)
                     /locus_tag="ACG827_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0484708.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(563..787)
                     /locus_tag="ACG827_002617"
     CDS             complement(563..787)
                     /locus_tag="ACG827_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0484709.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1242..1412)
                     /locus_tag="ACG827_002618"
     CDS             complement(1242..1412)
                     /locus_tag="ACG827_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484710.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1427..1960)
                     /locus_tag="ACG827_002619"
     CDS             complement(1427..1960)
                     /locus_tag="ACG827_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0484711.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1981..2925)
                     /locus_tag="ACG827_002620"
     CDS             complement(1981..2925)
                     /locus_tag="ACG827_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0484712.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3562..3648
                     /locus_tag="ACG827_002621"
     CDS             3562..3648
                     /locus_tag="ACG827_002621"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0484713.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3769..4458)
                     /locus_tag="ACG827_002622"
     CDS             complement(3769..4458)
                     /locus_tag="ACG827_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484714.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4465..4743)
                     /locus_tag="ACG827_002623"
     CDS             complement(4465..4743)
                     /locus_tag="ACG827_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484715.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4703..5617)
                     /locus_tag="ACG827_002624"
     CDS             complement(4703..5617)
                     /locus_tag="ACG827_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0484716.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5599..5979)
                     /locus_tag="ACG827_002625"
     CDS             complement(5599..5979)
                     /locus_tag="ACG827_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0484717.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1612 a         1127 c         1027 g         2326 t
ORIGIN      
        1 cccccccact ctccacaata caaaaaagac tagcaaatgc tagccacttt ttgtaaagtt
       61 tcgcttgttt caccttcggt gaaattgttg caaatcaata attaggtaga gctacatttt
      121 tttcgttgtt caaagattct tatgccattt actattaata aatctataaa tatgataatt
      181 tttcggctct ataatttatg ggactgatga atcagaattg attcttcagt cccattttca
      241 ttttattcca aagaattaac tccaaatacc aatagaagcc catcctctat atacttgaga
      301 ttccttacta acaatttctt gtaattctat taaagattta ggtaatccaa atttatgttt
      361 taaaatatat cttccaagaa aactacttaa tttcatacta acttcaccaa cactttttcc
      421 ctcactgatt tctttagaat aaagaaagag cttttctttt aagtcaggat agtcatctat
      481 agttgcttca ttgtatactt tactaattgc atttaaaagt ttttcttctt tagtaaaatc
      541 cattataata acctccctac cactaccttc taggtgccca tggaccatga ttaacccaac
      601 cattaacaat aatttttcca atttctcctt tagcttgatt ccaatctacc caacattttt
      661 ctttgttaca ataaagacca tttccatagt aagtagctgc atcaactttt attcctgtac
      721 cgatgccagc tagacaagtt cctaatattc ctagaataac acaatgtttt aatactttct
      781 ttttcatgat tgtttttcct tttcgttttt ttaaaattgc ttgttatatt aaataatctt
      841 agtaatttta ttatataaca ttttattata taacaatgat taacgtcata aaaagtagtt
      901 ttgaattcat aatcgtttaa ataaaattat aagatcagtt atttaaacag ctagtagaca
      961 taatgtgtat actaccaagt aacgcctgta ccaagggatc caggcgctga ttttgtgaat
     1021 ttttattggt ctaggttaaa agctcttttt aaggcttggt tttctgcggt tgtttctttc
     1081 taattctttg tgaatattga tcggtcattt tttaaatact tgttattttt gtttgtgttt
     1141 gttatgcttt ttacatatct catattcatt tattattctt ttctaaggtt aataatagga
     1201 taatctcttt cagaaagact gctcgtacga gcagtctttt cttatttatc aagtttttct
     1261 acaaatactt cctcattttg actgttgtag taattaggat tgagttttag ttcccctttt
     1321 ttatttcctt tgttggccac tgataataac attgggctaa agcttaatat tcctagtagg
     1381 atagaaccga atagcaagtg tttttttgtc ataagtattt ccttcttcat ttccatagct
     1441 gccaccattt tttccaattc tttgtttgtt cgtccattgt ttctgttgct tcagaaagag
     1501 tcgttgaact attctctgaa gactcttccg tataagtcaa ctgcagttgc tcctgaagtt
     1561 tttcgatttg ttggttggct tgcagggtga gttgttgttg ctggtcgagt agtttttgta
     1621 tatgagaatt attttgctct ttgagggcaa gttgttctcg taaaaattca ttttcttcac
     1681 gcaactgatc tagcaacttg tttgcaacca cgtttttgtt tggtatataa gcatttttat
     1741 cgttgttttg ttcatgttta tgcaacttat ctagcaacct ttgattgtct aagttgcgag
     1801 caagttgcaa tactccagaa cgcaatacaa ccttataacc acgctcattt ttagctatca
     1861 tggaagaatc taatttttgg atatgtttcg atattgcttg cctactcatc ttaaaaagat
     1921 ctgacaattc ctttattgta aaggtttctt gtggttgcat actggttgcc tcctaggttg
     1981 ctactggaat aaattatcta aatcagataa taaacttttt ttctcttttt ccgaaagtgc
     2041 ggactctgtt tgtttttcaa gagctttttt ctctttttct agttgtttga tttgttggag
     2101 gagttcaagt tctcgtttct tctgtttgat tttttcgtta attgttgtag cgggttcttc
     2161 gtattctttt tgtttatcag ctaaagccaa tgctttattt tggtcttctt tggttggtgc
     2221 atgtttgtta aagtcgtctg cttttttgat ctcaggtttg aacgtaaatt gatagccaat
     2281 aacaggtttt ccccgtcctt ttccgtattt ctttttgatt gttagtcctt tgaagattgc
     2341 agttagttct tgacgtatag gttttataac tcgtttatct atatcaggtt gcttataact
     2401 tttaggaata tctaataatt caagaaagtc ttctttggag aaatatgcga aacctttggt
     2461 tctgtattgc tttaacagac gaaacatagt ctttgcatag ccactttgta gctcgttgaa
     2521 ttgttgtaaa gaatagcgta cccactcgtc taaattattt aacaaaggca gtgctttttc
     2581 gtaaattcgt atatctacgt aaggattttc tacgtccccg ttgattttaa attctgtaaa
     2641 cattacaaac atatcgtaag aaagcccaga ctcacttcta cgaccaaagc gtaaagataa
     2701 gattttttca taagttgctt ttaaatcttc tacaaatcgt acatttgcag ttgctttgta
     2761 gttacttaaa tcttttagtt ggtcaaacgt aaaacgaact gtcttatccc ctttttcacg
     2821 cattctagac acgatggaaa aaaacaaatt catttcaacc ggtgtaaatt ttcttaaagg
     2881 aattgtattt aattcatggt ggtattttac aatctcatta gccatataat cacctcaatc
     2941 caattatgaa tgacattttt taaatgtcaa gacattcata tttgacgttc aaacagacaa
     3001 aatgtccttc ataaacagac aaaatgtcct tcataaacag acaaaatgtc cttcataaac
     3061 agacaaaatg tccttcataa cattctgtat ccattggtag agtaaggaca cagcttacgg
     3121 taaagaggtt taaaaagagg tttaaaaaga gatataaaaa gaggggggct aattttcccg
     3181 cctaaaaacc acttcttttt tctttaaaca ggttcgcgca tttttcaaaa ctgccaatcc
     3241 aaaaccttgc ttcctacggt cgccaaaaag gtgtgatcgc tacgctctca cattgtactg
     3301 cgcagtcgcc taaaggctcc tttgctaaaa acatgttggt aaagaggcct ttgagcggtt
     3361 ctgaatgctt atctaagccg ttttaagaca gtcttggata aaaatcctta tctagcttta
     3421 aagcgcctta caggcggtca gggcaagccc taacaaccct aaaataagcg atacttaaaa
     3481 acaccacaca agtttaattt tttaagatat aataacttgt cgcctatccg acagtcttgc
     3541 gactggaagg aggtgtatct aatgtttgaa ttgttcattt gcccccttct tatagaagtt
     3601 tgtgtctctt tattcagtta ttggttgaat aaccgaaaca agaagtaaaa tcaataaagg
     3661 cgacatcaac ctgaaaaaaa gccccagaga atccgacctc tctggggctt tcgtgtatct
     3721 aatgtttgat catttgcatg tttagtttaa catgattgcc gatcaaattc aattttgctg
     3781 ttgctgatct aaccattcct tgacactttt tagataattt aatgccgttt cttttttcgt
     3841 tggattttgt atgttcgttg cttgctgaag cagtgcttta taacgttcgt aggtaactga
     3901 ttgttgccgt tcttttcgtt cctgttcact agttgaaaaa gggattgtat cactgaataa
     3961 attataacaa gcttcttctg tctgcgtatc tttaaaatat tgcacgtctt ttttggttcg
     4021 ctcatagttg tcttgaacct ctttttgctg tttttctggc aagttttgat aaagacgagt
     4081 gagtttttcc atgtttttat ccgtcaaacg tttttcttga ttcacaaaaa tatcgttcat
     4141 ttcctcataa caagtggtaa actcttccat tttttgtgtt aacacttgct cctttttctt
     4201 tttatcacat gctgcttcat acttttgagt gatatcccct aataaaatac ttcttgaaat
     4261 acgtttattt ccctctaaaa cgccgaataa accacttaaa acgattgata atgtaaatat
     4321 aacccctggc aataaaaacc tcttaaatcg aattttagag ccttttaatg aatatcttgt
     4381 ttgataagcc ttatagctaa gccaaataat tcctaaaata ctggcaaaaa tgagtagata
     4441 gggtaaatag aatgccattt ctatttattc tccccatacc agccaaaagg ctaacagtgt
     4501 catgattaag caacataagg gggcaagaat atccattgat cgctgcataa ttactttttt
     4561 ctgttcttta tttcgctggt ttaatagcgc atggtggaat tttttgactt gtgaatcaat
     4621 tgtagtttgt attctttctt tggtataagg tgcaagaaaa gcacgttgtt tctttatttc
     4681 attttctatg tcttgtaacg ttttagaacg ataattcttg ttggtagtct gctcctcttt
     4741 caacgtgttg gtcatggttt cttcgagtcg gtctaatttc cggttgttca gtcgtaagag
     4801 ttcgttgttc tcttcgtact tcttttgcag tttcgcgaat gctagctcga gctcttcgta
     4861 gctcgttggt tcgttgttga ttgtaggctc gttctcgtct gtccagctca tgtaaaatcg
     4921 tctccttttc gtaatccttt cctagtttgt tggatcgtac ttttttcttt ttgtggatca
     4981 attcgtaagt aaaatttttc ccacgattca agcaatgaat gccgaatgtt ttaagagaat
     5041 cacaaaattg gttatacgtt cgagtttttt tgtttttcat tgtttgatca atcgcaaacc
     5101 gaatttcgtc tttccaaaca ggttcatttt tttcttttaa tttgatttca gacatggttc
     5161 gtttttcggg tttcttccct cgttcagtga ttgttaagtc atgttcaaga caaatctcgt
     5221 catttaggtc ataacatttg tttaaaaatg cttgaccatg ggcttgaaat ttgttcccag
     5281 tttccagatt gacggagtta atgacaatgt ggttatgaat atggtctttg tctgtatgtg
     5341 tgtaaatcgc gatttggtgg tcaggggcta tttttttcgc atactctgcc cctagttgat
     5401 tggctttttc agcggtaatt tctcctggtc gaaaagactg gatcaataag tgtgcttgta
     5461 cacgatcgtc ttttccatac agcatgcgaa tttgtttcat ttcgcttttg gcataatcaa
     5521 tgtcacagtt tatccctgtt ttgacggttg ctcgtggttc ggcgtaattg atcgctcggc
     5581 tacaagaagt ggttcggctt actttaactg ttgccatatc ttttgtagct ccttttgcat
     5641 gatttttagg ttttcggata agcgttgggc gtcgccagga tcaacgtcgt gttggtgtgt
     5701 gttgcaccac ttggcaattt gattgatatt cccgccagct ttggctaaat cgcgcgcgat
     5761 tgcctgagca atgtctggcg ccactttcgg tgtcaccaga cgcgcgtttt gtgccttggc
     5821 tttcacgaaa gccggcacac tcatttgcaa agcttcagct gactgcttca gctttgcaaa
     5881 ttcggattcg ctcactcgaa aactgatttg ttttggctcg gatcgatagg tatatttttt
     5941 agaatgattg ctagccaaat attgattttg ttcgctcata agtcccaccc tttcagtacg
     6001 tcctacggac gtatcgttcg caataccatt gtacctaaaa aggtacaata agtaaagctc
     6061 actaaagctc actaaagggc ttggcaagct ct
//
LOCUS       DAZKOU010000119         5949 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000119 DAZKOU010000000
VERSION     DAZKOU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.122"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(175..633)
                     /locus_tag="ACG827_002626"
     CDS             complement(175..633)
                     /locus_tag="ACG827_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0484718.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(646..1977)
                     /locus_tag="ACG827_002627"
     CDS             complement(646..1977)
                     /locus_tag="ACG827_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0484719.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1988..3430)
                     /locus_tag="ACG827_002628"
     CDS             complement(1988..3430)
                     /locus_tag="ACG827_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0484720.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3417..4181)
                     /locus_tag="ACG827_002629"
     CDS             complement(3417..4181)
                     /locus_tag="ACG827_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0484721.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4285..5160)
                     /locus_tag="ACG827_002630"
     CDS             complement(4285..5160)
                     /locus_tag="ACG827_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0484722.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5415..5499)
                     /locus_tag="ACG827_002631"
     tRNA            complement(5415..5499)
                     /locus_tag="ACG827_002631"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5463..5465),aa:Leu,seq:caa)
     gene            complement(5517..5588)
                     /locus_tag="ACG827_002632"
     tRNA            complement(5517..5588)
                     /locus_tag="ACG827_002632"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5554..5556),aa:Gln,seq:ttg)
     gene            complement(5598..5670)
                     /locus_tag="ACG827_002633"
     tRNA            complement(5598..5670)
                     /locus_tag="ACG827_002633"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5635..5637),aa:His,seq:gtg)
     gene            complement(5732..5816)
                     /locus_tag="ACG827_002634"
     tRNA            complement(5732..5816)
                     /locus_tag="ACG827_002634"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5780..5782),aa:Leu,seq:caa)
     gene            complement(5837..5907)
                     /locus_tag="ACG827_002635"
     tRNA            complement(5837..5907)
                     /locus_tag="ACG827_002635"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5873..5875),aa:Cys,seq:gca)
BASE COUNT         1829 a         1260 c          901 g         1959 t
ORIGIN      
        1 aatttatttt taagaatcct gaaaataagt tttctaaatc ataggagcaa caaaaaaatt
       61 cgataattaa caaaaatcaa aatgattaaa tctagtgatt gaaacatatc atttcaaaaa
      121 aattttgtaa attcacccat aaatatcacc ctatttaact tttttcatcg tacactaaaa
      181 ttcaaaacaa ctatctttaa tctttacttc ttggttttgt tttaataaat ttccttcaaa
      241 aatgcctgga gaaactacaa tgatcaccgt atctttttgt gcttgctcca acttcttcaa
      301 atctataata gctaacagtg tcccttcttt tacctcctgg ttactttcaa cccgtatctg
      361 aaacggactt ccttcgagtt caactgtatc taaacccata tggatcagta tttttctgcc
      421 agattcactt tcgatcgtta tggcatgtcg ggtaggaaat atattatcga ttattccttt
      481 aactggcgca tggatatatc catcatgatt cgttatagcg tatccaactc ctatcatttt
      541 tgaagaaaag acctcgtctt ttactgctgt taatggaata atttgaccac tagctacagc
      601 ttttagaatg tttcttgttt taaagaaatg cactttttat cacctctact ttaatgctgg
      661 ccaatatttt ttgtttactt tgatcatttc atctagtatt ttttttgcga ctctggcatt
      721 tggtacggtt tgattcaatg taaatgcttg taaagctttt tgataagaac cttcaaagta
      781 agcatctact agtaacttct ctactgcaac ttgtgcctcc atcaatcctt tatgaaaatc
      841 actgattggt tttcttagtg taattggctc cacaccagtt gcaccaacat atgccggtat
      901 ttcaacaact gcatctgacc gtagattcgg aatcgcccct tgattaggaa cgattagcat
      961 aaagcgacga cgatcatcat tcaacaagga aatagctata tcgacgatat attgtccatg
     1021 ctccccaaaa ttaaagttca atacttcttc cacaataccg cggcgtattt tatcagccat
     1081 ttctttggta tttttctctc gtccatccat gaccatatta gcacgtgtat actctttgtc
     1141 agtatgctca acgaccatat caggatataa gtaatactct aagtaattat tcgggatatt
     1201 acttggaaaa tttttcgtga tgactgacat catttgaaag gttttttgcc aagttttatc
     1261 tcctatatta aaatcggcga cttgcatttc ttttgttatt agtttttcaa taatctctgg
     1321 cataatttct cgttgcaatt ctttatcata aatcgatgta taccagccaa agtggtttaa
     1381 accataatac gtaacgatcc aattcttacg atcataaccg taattcactg caatcgtttc
     1441 ttcaattgaa atagtcatat cacaggcatt aatcattttt acattcggat atttacgacg
     1501 aacagcttca gaaacgatcg actctggatt tgtataattc aaaatccatg ctttgggggc
     1561 atagtcttgt atatgatcga caatttccaa taaacctttg attgagcgca tcccataagc
     1621 aaatcctccg agtccacacg tttcttgtcc tactaatcca tgtttaagtg gaatcttttc
     1681 gtccttttct cgcatctcca atcctccaac acgaatttgt gagaaaacaa aatcgcatcc
     1741 tgtaaaagca agctttggat catccgttgc acgaatgcga atatttgaaa aaccttttct
     1801 tttcagcata aatttaacaa tcaagtacat atccatattt ctttcttcat caatatcata
     1861 taaacgtatt tcagataatg gcaaccgttt ttcattgttc aaaatggctt gaacgattcc
     1921 tggtgtatac gtactcccgc ctccagctat agtgataatc tgttttttca tcatcatctt
     1981 ttcctttcta ttcttctaat aatttattga attctgccat aacataagtc acttctaagc
     2041 cgatgataat ttgataattt ttttcagttg gtttaatgat cccgcttatc cctaattgct
     2101 tgattcgctg ttcctcgacc atggtgggat ccactacctc tacccgtagg cgtgtattac
     2161 aactatagac atccaggata ttggcttttc ctccaaacgc ttgtagcatt tttgttgcca
     2221 tatattcata atttttactt gctaacccta actctgtttc ttctaaggtt acttcattgc
     2281 caaactctgc cttagcttca cgtcctaatg tctgaatgtt tctcttattg atcaaataat
     2341 aaaaagtaac gtaatacaaa gcaaaaaaga aaattccgat tggaagaatc aaaatagcat
     2401 tggtagcgat tcgaaaattg atgagataat caacaataca tgctccgaat gtaaaaccaa
     2461 ttcgtatatt gaataagtaa cagacaatcc ctgctaaacc ggcaaaaagt gcatggatca
     2521 cgtacaacat aggagaaaga aacatgaaac taaattcaac aggctcagtg atatttgcaa
     2581 taaatgaagt tcctgcaccg cttcccatga ttcctttgac catctcttta ttttctttat
     2641 aagctgcttt atagatagct agacatatag ctggtatacc aaacatcatt actacaaaaa
     2701 aaactgataa aaacagtcca gcctgaggat ctccgttgat aaagcgtgtc atctctcctc
     2761 tgaaaacttc cttgctagga gatgtatagc ttcctaaatc gtaataaata tacgtattca
     2821 acacatggtg cagtccaaag gggatcagca aacgattcaa aaacataaat atccccacac
     2881 ctaatgctcc catatcaacg agcagttttg caaaattatc aatcccaatt tggatgagcg
     2941 gccagataaa gcccaaaaaa acagctgcga ttgtttgtag aatcatcacc atagtcaatg
     3001 ggaatttttc gcccgcaaaa taagaaaaaa ctactggtaa tttttgttct ttaaagcgat
     3061 tatacgtcca tgccgctact aaaccggaaa taattccacc aaaaatcccc atattcactt
     3121 ctgggtccat aatttctaag ccttttttca aggtagcaat cgctaaaaaa ccagtaagtg
     3181 caggtattcc tttgtcctta tttttgacaa aaccgattgc aactcctata gcaaataaga
     3241 tatccaagtt tccaaaaacg gcatttccag ctgcaccaat tactgggatg ttcagcatat
     3301 cgtcagctga taaacgcatg atcaatcccg ctaatggcat cgctgcaact ggaagtagca
     3361 tcgctcttcc aagcttctgc acatttgatt taacattcat ttttattcct cctctattaa
     3421 atatcctcat atgttactaa ctcaatcaaa ggatcatttt tcactttttg agcaaatgct
     3481 gaatgaatta aaaattcgac ttggtgggta cgaggataca caaaacttga accatttaac
     3541 aattcctgat ccaaatacgc tgcgtgacac atgacttcga tcgttttatc ttctctcaat
     3601 atcatgagta aacttgccaa atattcttcc acactcgtat tgatttgtcg ttttttttca
     3661 tcagtttctc ctacgacatc gaaacaaggc tcaaaataat cgacatgacg aacttcatct
     3721 tttcgttcaa agttgctccg aaccggcaaa tcatattttt ttgctaatgt aacaactacc
     3781 tcctgatttt catccaaagt atgagtatga tgatgactgt ctaaatgagt tggtatgatc
     3841 cccgcatgat aaattttttg aatctgagca ttccattcct gatacagttg atctttgtca
     3901 atgttaaatg gatgctgata ataagataag gatctgaact ctccgctttc gataagagta
     3961 tcgacagttt tgaggagtgg tttgccacac gtcaatgtta aatggactcc tacacctagc
     4021 ctaggcattt cctttcttag tttcacagca tgctcgaaac ccggcatatt tgccatcaat
     4081 gtagttgatg tcaaaattcc tcgctgataa gcatccataa tcccataatt cacaccatgg
     4141 ctatagccaa aatcatccga attaaaaatc acttttccca tttcattctc cttctttttt
     4201 ctactaaaat aggcgcaacc aaataatcat agtccattta ctatgtgtca tttggttgcg
     4261 cctatagata gttacgccct tattttaata gattcttgct agatgaattg ccaaatagat
     4321 gatttcattt tcttgaatgg caagttgaaa ttgctgttcg atgatatctt taatctttag
     4381 tgctgtacaa tacgctttgc tatgtttaaa tgtttcagta aaaaaaccaa tattttctac
     4441 tggcgtttct tcctgataaa tcaagcgttt aaccaggaat ttgatatgaa ccattaagcg
     4501 atgataagtc agtgattctt tatcataaac gtttgaatag gatttttcta gatgggacaa
     4561 aatcgtggtc gtcatttcta ctatttttaa gctctttaaa ctattaggct ctcctagctc
     4621 agcattaatg atatgcatag tgatgaatcc tacttcattt gaccctaact cggtttgata
     4681 tcgatgattg attcgctcga ccacttctac tgctatatca tattcatttg gataaaggaa
     4741 tctcatttct ggcaacataa ttgctgtgac atctctatct tctctttttc gttgaatagc
     4801 aaaataaata tgatctgtca atgtaatata gatatttgaa tctaatggag aggagatcct
     4861 agctgttatc agttcaatca cttcttccgc aaaatccata aattcgattg gaatctgcga
     4921 gactatctca ctgaatcgtt tgatcttttc tttagaatct aatacaaata atttgtcagc
     4981 atcctcaaca tcaaatggct tactcacttt agcagaaaag ccaatccctt tccccatcgc
     5041 aattttttct attccatcgt catcgatcaa tacagcatta tgattcagct ttttcatcac
     5101 atacaccgtt aattcacctc ctctatatga taaaacaaaa caacttgcag actccttcat
     5161 acatattttt gtctccaaac aatttttaaa gacaaaaaac gacctaagaa agaacccttt
     5221 cttaggtcgt gcctaattga atagtagcac cctacataat ttacaaaaat agtttaacac
     5281 ataataaaag cgttatcaat atctttttta ctcgttttat gataatactt gtatcgaatt
     5341 gatttggagg taattacata aaaacaaaaa aaatatagca actctctata agaaagttgc
     5401 tatattgata attataccgg cggccggggt cgaaccggca cgtcctcaac ggacactgga
     5461 ttttgagtcc agcgcgtctg ccaattccgc cacgccggca aatttaaaaa taataactgg
     5521 ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct
     5581 ataccccaag aataaaaggc gaatgatggg aatcgaaccc acgaatgccg gagccacaat
     5641 ccggtgcgtt aaccacttcg ccacattcgc catcataaaa gatgttattt aatttctcaa
     5701 taaaaaacta agatgaagac atcttagtca ataccggcgg ccggggtcga accggcacgc
     5761 cctcaacggg cactggattt tgagtccagc gcgtctgcca attccgccac gccggcaagt
     5821 tgatttatta gtactaaggc ggtaaccgga tttgaaccgg tgatgaaggt tttgcagacc
     5881 tctgccttac cacttggcta taccgccatt atactattat caatctataa aataaagaat
     5941 aaattatag
//
LOCUS       DAZKOU010000120         5816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000120 DAZKOU010000000
VERSION     DAZKOU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.123"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(233..1015)
                     /locus_tag="ACG827_002636"
     CDS             complement(233..1015)
                     /locus_tag="ACG827_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484723.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1080..1916)
                     /locus_tag="ACG827_002637"
     CDS             complement(1080..1916)
                     /locus_tag="ACG827_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0484724.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1934..3343)
                     /locus_tag="ACG827_002638"
     CDS             complement(1934..3343)
                     /locus_tag="ACG827_002638"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0484725.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3357..5180)
                     /locus_tag="ACG827_002639"
     CDS             complement(3357..5180)
                     /locus_tag="ACG827_002639"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0484726.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5460..5687)
                     /locus_tag="ACG827_002640"
     CDS             complement(5460..5687)
                     /locus_tag="ACG827_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484727.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
BASE COUNT         1784 a         1264 c          948 g         1820 t
ORIGIN      
        1 aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat
       61 atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct
      121 ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta
      181 cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa
      241 taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat
      301 aatctttttt cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca
      361 ctgcattact gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag
      421 aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag
      481 gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag
      541 tatctacttc tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg
      601 taactgcttc tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt
      661 gtccatcttt agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac
      721 cagttctttc aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga
      781 catgtaaaag cattttgttg tttacatcta cataataatc ctttgactct acttgagacc
      841 ttcctatacg gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa
      901 tctgccaatc tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca
      961 cttcactggt agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat
     1021 caggagcctt agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt
     1081 cagtacaccc ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca
     1141 cccatctctt cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa
     1201 cgctgcttca ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc
     1261 attcgggcaa taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta
     1321 tcaacctttt tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt
     1381 accgagagca tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac
     1441 gcaatgtttc cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg
     1501 atagcatagc agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg
     1561 cgattttgaa aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc
     1621 aaggtaaaaa atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata
     1681 attcgcttga cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc
     1741 ttttttagag catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga
     1801 accaaatcgc ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg
     1861 ccttgcttat ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga
     1921 ttcaccgttc tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc
     1981 tttctttttt tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa
     2041 aaccataacg cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta
     2101 aataaccaaa aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg
     2161 ctagataatc aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg
     2221 ccccatagcc attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca
     2281 atgttaatcg tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag
     2341 gattttcagc tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc
     2401 tagcggaaac atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat
     2461 taatcgcaat aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt
     2521 taggatataa tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa
     2581 ctgctacatc taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat
     2641 cttctggact ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc
     2701 ctttaagatt caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt
     2761 gattaatttg aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg
     2821 ctccaggatg aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt
     2881 caccatactc agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat
     2941 ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa
     3001 tcggttcgat tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct
     3061 tttgattggt tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa
     3121 aagcttttaa gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat
     3181 ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg
     3241 gttgtttccc atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc
     3301 ataaaaactt ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa
     3361 ctaatttgca tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg
     3421 taattttcac tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg
     3481 gcagcgaaat cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca
     3541 aatacttcaa agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat
     3601 tcaatacctt ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact
     3661 tctccctcaa caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc
     3721 aatagtccct tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt
     3781 accggatttt caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta
     3841 tcgtcaaatc caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta
     3901 ataattgcgt gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag
     3961 ccaggggaaa caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt
     4021 gcaccaccaa tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt
     4081 gaaggctcag taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac
     4141 tctttgtttt tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa
     4201 gccagcattc ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg
     4261 attggcgtca ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc
     4321 aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga
     4381 tcgttcagca acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa
     4441 aataccagca tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag
     4501 ggctctacat aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa
     4561 tctgcagcta ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt
     4621 gaaatatttg gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca
     4681 aactttatgg ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca
     4741 atagtattca aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag
     4801 acggccaaaa tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag
     4861 gtggtagaaa tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg
     4921 ccctttttaa ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca
     4981 ttgccaatca ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt
     5041 ttttctaagg ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg
     5101 gcacaatggg ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt
     5161 cctacttctc tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa
     5221 ccacgcccca atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa
     5281 catgccaata acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa
     5341 cagggaattt aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc
     5401 acatcaacta gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac
     5461 tataccagta aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc
     5521 aaaaatgcat ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga
     5581 atttggtgag taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata
     5641 cttgtttttt tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa
     5701 ggtatcgata gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact
     5761 gatttatttc taatctacat gtagtaagta tttcaattcc accaattatc aataaa
//
LOCUS       DAZKOU010000121         5689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000121 DAZKOU010000000
VERSION     DAZKOU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.124"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG827_002641"
     CDS             complement(488..1297)
                     /locus_tag="ACG827_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0484728.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG827_002642"
     CDS             complement(1297..2043)
                     /locus_tag="ACG827_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0484729.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG827_002643"
     CDS             complement(2061..2528)
                     /locus_tag="ACG827_002643"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0484730.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG827_002644"
     CDS             complement(2528..2938)
                     /locus_tag="ACG827_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0484731.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG827_002645"
     CDS             complement(2949..3962)
                     /locus_tag="ACG827_002645"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0484732.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG827_002646"
     CDS             4179..4913
                     /locus_tag="ACG827_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0484733.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACG827_002647"
     CDS             4897..5607
                     /locus_tag="ACG827_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0484734.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAZKOU010000122         5481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000122 DAZKOU010000000
VERSION     DAZKOU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.126"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG827_002648"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG827_002648"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ACG827_002649"
     CDS             complement(580..1353)
                     /locus_tag="ACG827_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0484735.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ACG827_002650"
     CDS             1497..1847
                     /locus_tag="ACG827_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0484736.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ACG827_002651"
     CDS             1828..2544
                     /locus_tag="ACG827_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0484737.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ACG827_002652"
     CDS             2544..3992
                     /locus_tag="ACG827_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0484738.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ACG827_002653"
     CDS             complement(4062..4571)
                     /locus_tag="ACG827_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484739.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ACG827_002654"
     CDS             complement(4561..5286)
                     /locus_tag="ACG827_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484740.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DAZKOU010000123         5358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000123 DAZKOU010000000
VERSION     DAZKOU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.127"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..926
                     /locus_tag="ACG827_002655"
     CDS             69..926
                     /locus_tag="ACG827_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0484741.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1041..1961
                     /gene="pstC"
                     /locus_tag="ACG827_002656"
     CDS             1041..1961
                     /gene="pstC"
                     /locus_tag="ACG827_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0484742.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1961..2845
                     /gene="pstA"
                     /locus_tag="ACG827_002657"
     CDS             1961..2845
                     /gene="pstA"
                     /locus_tag="ACG827_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0484743.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2860..3666
                     /gene="pstB"
                     /locus_tag="ACG827_002658"
     CDS             2860..3666
                     /gene="pstB"
                     /locus_tag="ACG827_002658"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0484744.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3682..4440
                     /gene="pstB"
                     /locus_tag="ACG827_002659"
     CDS             3682..4440
                     /gene="pstB"
                     /locus_tag="ACG827_002659"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0484745.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4453..5130
                     /gene="phoU"
                     /locus_tag="ACG827_002660"
     CDS             4453..5130
                     /gene="phoU"
                     /locus_tag="ACG827_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0484746.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
BASE COUNT         1689 a          954 c         1195 g         1520 t
ORIGIN      
        1 ttgattcgtt ttcatcaagt aatgaaaatg ataaaataaa catactttag gattaaagga
       61 gatttctaat gaaaaaactt aaattattct taccaataat aggattagcc attcttttat
      121 caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc tcttctgctt
      181 tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct ggaaaattca
      241 tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca ggagcagtcg
      301 acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc tcagcattag
      361 tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat attggcgtaa
      421 aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa aactggagtg
      481 aagtcggtgg aaaagatcag ccagtagtta tattgaaccg cgcttcagga agcggaaccc
      541 gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca caagaacaag
      601 attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc agctatgttg
      661 ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta gaaccgacag
      721 atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg tacacaagag
      781 atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat gaagtacaag
      841 ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc gaacgtgatt
      901 ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt agctaaactt
      961 ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt atgttaaacc
     1021 tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca aaatcaaaac
     1081 gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg ctgatcgtgt
     1141 tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc ttttttactg
     1201 ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc caaatcggag
     1261 caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt attgtgacct
     1321 ttttatctgc tatcgttgcc acgccatttg ccattggtgc agcagtattc atgacggaga
     1381 tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt gtaggtattc
     1441 cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc agaagtattt
     1501 ttggcgggac agggtttggt atcttagctg gtacatttgt gttgttcgtt atgatcttgc
     1561 cgactgtcac aacgatgact gttgatgcat taaaggcagt accacggcac tatcgggaag
     1621 cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg ctacgagcag
     1681 ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt ggtgaagcct
     1741 tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggactt actactcctg
     1801 cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg ggaacagttg
     1861 aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta ttctttaata
     1921 tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa aacgctcaga
     1981 taaaattgct acaggtgttt tatatatagt ttcggggatc atcgtattga ttttagcagc
     2041 tttactactt tatatcttag ttcggggaat accgcacatt tcatggtcct tcttgaccga
     2101 accttccaaa gcctaccaag aaggtggtgg aatcgggatc cagctattta actcgattta
     2161 tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg gtatctatct
     2221 ttcagaatat gctggaaaaa actggctaac agatgtgatt cgcacgtcga ttgaaatctt
     2281 gagctcgttg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg ttattcaatt
     2341 ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca acttgcctct
     2401 tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac gagaagcagg
     2461 attggcctta ggtttgtcac gctgggaaac agtgatgaaa gtggtggttc cagaagcgac
     2521 accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg aagcagctgc
     2581 attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact ggaatccatt
     2641 aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg tccatatttg
     2701 gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg gcgcttctgc
     2761 cgtgttgatc attgctgttt tactgttcaa cttcggcgct cgtgcgattg gcaaacgatt
     2821 ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata taactggaat
     2881 gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac ggatgatata
     2941 catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt tgaaaaaaat
     3001 aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct tcgttcgttg
     3061 aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat gtatcaaggc
     3121 gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat tggcatggtt
     3181 tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt tgctttgaaa
     3241 cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag cttgaaacaa
     3301 gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc tttatctggc
     3361 gggcaacagc aacgtttatg cattgctcgt gcgatcgcga tgaaacctga tatcttgctg
     3421 ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga agaaacactt
     3481 gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca acaagcagct
     3541 cgtatcagcg actatactgc ctttttctat ctaggaaaag taatggaata tgacaaaact
     3601 aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc cggtcatttt
     3661 ggttaagacg gaggcctcaa catgacaaaa gaaattattt catctaaaga tcttcatctg
     3721 tattatggaa agaaagaagc actaaaaggg atcgatctaa gtttcaatca aggggaaatc
     3781 actgcgatga tcggtccttc tggttgcgga aaatccactt atctgcgttc tttgaaccgg
     3841 atgaatgacc tgattccgaa tgtaacgatc accggaagtg ttttatataa agacaaagac
     3901 atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat ggtattccag
     3961 cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt gaaattaaaa
     4021 ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa ggcagcgtct
     4081 gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc cggcgggcag
     4141 cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat cttattggat
     4201 gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat gctgttagaa
     4261 ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc ttcacgtatt
     4321 tcagataaga cggcattctt tttagatggt cacttgatcg aatacaacga tacgaagaag
     4381 atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg atttggataa
     4441 ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct ccataatcag
     4501 ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg tgcatacatc
     4561 aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat caataacatg
     4621 gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc agttacaact
     4681 gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg tatggccgat
     4741 catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag aataccggaa
     4801 atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga caacgtccta
     4861 gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat ggatcaaaga
     4921 gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca ggaaaatcct
     4981 gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga acggatcgga
     5041 gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa aatcactgag
     5101 ttaaatacga accacaatga gattttctaa actgaagctg tgattatgcc gtttgtttat
     5161 gaaaagaggt tgtgaaaaag ctgttctaca tcaagtaata agaacagtca aacaaaaaat
     5221 agctcctcat atttttcgtt tggctggact tattaccgca gatgcaagct tttgaacacc
     5281 gtttattcgg gaaagggttt gtgacaaaac atgtcacaaa cccttttcat catttcatag
     5341 aaaggataaa gaatgcaa
//
LOCUS       DAZKOU010000124         5311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000124 DAZKOU010000000
VERSION     DAZKOU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.128"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG827_002661"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG827_002661"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0484747.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG827_002662"
     CDS             complement(1297..2133)
                     /locus_tag="ACG827_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484748.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG827_002663"
     CDS             complement(2317..3534)
                     /locus_tag="ACG827_002663"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0484749.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG827_002664"
     CDS             complement(3683..3844)
                     /locus_tag="ACG827_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484750.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG827_002665"
     tRNA            3917..4000
                     /locus_tag="ACG827_002665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5311)
                     /locus_tag="ACG827_002666"
                     /pseudo
     CDS             complement(4240..5311)
                     /locus_tag="ACG827_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca t
//
LOCUS       DAZKOU010000125         5273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000125 DAZKOU010000000
VERSION     DAZKOU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.129"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..273)
                     /gene="prgO"
                     /locus_tag="ACG827_002667"
     CDS             complement(<1..273)
                     /gene="prgO"
                     /locus_tag="ACG827_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0484751.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(245..1198)
                     /locus_tag="ACG827_002668"
     CDS             complement(245..1198)
                     /locus_tag="ACG827_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0484752.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1810..3303
                     /locus_tag="ACG827_002669"
     CDS             1810..3303
                     /locus_tag="ACG827_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0484753.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3438..3734
                     /locus_tag="ACG827_002670"
     CDS             3438..3734
                     /locus_tag="ACG827_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0484754.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3835..4686
                     /locus_tag="ACG827_002671"
     CDS             3835..4686
                     /locus_tag="ACG827_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484755.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4707..4919
                     /locus_tag="ACG827_002672"
     CDS             4707..4919
                     /locus_tag="ACG827_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484756.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1799 a          821 c          997 g         1656 t
ORIGIN      
        1 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
       61 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
      121 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
      181 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
      241 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
      301 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
      361 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
      421 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
      481 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
      541 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
      601 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
      661 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
      721 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
      781 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
      841 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
      901 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
      961 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1021 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1081 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     1141 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     1201 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     1261 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     1321 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     1381 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     1441 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     1501 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     1561 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     1621 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     1681 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     1741 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     1801 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     1861 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     1921 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     1981 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2041 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     2101 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     2161 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     2221 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     2281 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     2341 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     2401 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     2461 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     2521 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     2581 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     2641 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     2701 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     2761 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     2821 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     2881 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     2941 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3001 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3061 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     3121 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     3181 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     3241 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     3301 tgaaaaataa aaggtgcaaa ttgccctata caatatatct atgatacttc tgtaggtgtt
     3361 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     3421 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     3481 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     3541 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     3601 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     3661 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga aaaaggaaga
     3721 agtatatcca gtagtagttt ttatttactt aaaaaattgg ttcattgagc aacctatcgc
     3781 tgctaaagta gcaaaggaaa acgttaaagg aaagaattta gggaagaaat cgaaatgaaa
     3841 tcggacgact ggattgaaca cacctggctt cctctgaaaa gaaatagcaa acgaaagaaa
     3901 ttttgggtat tgcaattatg gccattagga aaactttgta gttacggggc acgacttatt
     3961 cttgatttaa ccgtttactc gattggagta tttggaacgg cttattactt gataaatgat
     4021 cccaatagga ttgcagcatc agacattttt acagtgctaa tgacgttgat ttttgtcatg
     4081 ctattgtcta tttttactca attagtcatg caatttacca ggcaaaaatt aacagcagga
     4141 aaacttttca aaagcctatt cgatttaatt gttgtaaatg caattctagc gactttaata
     4201 aaacaaattg aacaatctgg aagaacaaca aaaggagtga aggatttttt attgacgttt
     4261 attcactttg taaatggaca catgtatttg tttgaagtgg gtattattct cttttttgtc
     4321 gggtatgtat ccacgaaact tattaaacgt tcaattgaaa aagaaattga aattgacctg
     4381 attccgacac ttcttggtta tgagtacaat gttgattttg gaagatatga cgaattgatc
     4441 aatacggata tcgttcacac gagtatccat gaatctactg ttaagggcat cagatctatt
     4501 ttaaaaaatg gccaggaaat gaaagcaata gatcgaagaa aagagatcta caatttttat
     4561 gaagtcgaag caagcctacg atatggcatc ggaaaattgt ggttagatgg aagtaatatt
     4621 cgtcaaaaca aaccatacat tttttggaac agtagtgatt tagaacttcc aatgaaagat
     4681 aaataattat ggagaatcgt ttgaaaatga ttcctctata caggattgtg cttatagttg
     4741 gcatggagtt aaaaacctta aagaaggagc agttattcaa agtgaaagaa cgtcataatc
     4801 agttactatg tagtaaaacg ggtacgatga acattgaata ccgaacagaa tatttagaat
     4861 taacgaatca aactgatttt ttgttgattc gaaagcaaca agagcaagaa aaaaattaat
     4921 ggctacttat agccaattga ttgatgaatg ggaacaattc aaggaatcta aaattccaga
     4981 acgtctctaa aagcctttat ttgtaaagag cataaggaaa cgtgggaaca ctttttaaaa
     5041 aaagtgttcc aataggctgt gcctattacg tttctcaaaa ataaatgtgg gaacaaaata
     5101 tttaaaaata ggatagtgaa aggatagtga aggatagtaa aaggagggta gtaggatagc
     5161 aaaaaggata ggctcctttt cgctatcctt tttaaattgg aggaattaat atggaagtaa
     5221 gaataagaaa tgtggatgag aaaatcgtaa gagaaattga tcgaagatgt gac
//
LOCUS       DAZKOU010000126         5189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000126 DAZKOU010000000
VERSION     DAZKOU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.130"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACG827_002673"
     CDS             137..748
                     /locus_tag="ACG827_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0484757.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACG827_002674"
     CDS             821..1750
                     /locus_tag="ACG827_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFQ0484758.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACG827_002675"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACG827_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..3895)
                     /locus_tag="ACG827_002676"
     CDS             complement(2693..3895)
                     /locus_tag="ACG827_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0484759.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3975..4499
                     /locus_tag="ACG827_002677"
                     /pseudo
     CDS             3975..4499
                     /locus_tag="ACG827_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4706..>5189)
                     /locus_tag="ACG827_002678"
     CDS             complement(4706..>5189)
                     /locus_tag="ACG827_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0484760.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DAZKOU010000127         5095 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000127 DAZKOU010000000
VERSION     DAZKOU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.132"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..205)
                     /locus_tag="ACG827_002679"
     CDS             complement(<1..205)
                     /locus_tag="ACG827_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HFQ0484761.1"
                     /translation="MTIKWIISIAYLVLTFAIPSLGVPSNIYFLFEHSDIFFLVLIII
                     LFHSTFIREFKEVNLKKLFKYSFF"
     gene            complement(205..660)
                     /locus_tag="ACG827_002680"
     CDS             complement(205..660)
                     /locus_tag="ACG827_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="HFQ0484762.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1269..1685)
                     /locus_tag="ACG827_002681"
     CDS             complement(1269..1685)
                     /locus_tag="ACG827_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484763.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTITTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            complement(1687..1968)
                     /locus_tag="ACG827_002682"
     CDS             complement(1687..1968)
                     /locus_tag="ACG827_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484764.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(2222..2398)
                     /locus_tag="ACG827_002683"
     CDS             complement(2222..2398)
                     /locus_tag="ACG827_002683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484765.1"
                     /translation="MTEKTIKDGEKMVAILAIKPAIFINLNCWFYSSFCFCEQSNMDP
                     CCDILVAPQSPFFR"
     gene            complement(2415..3383)
                     /locus_tag="ACG827_002684"
     CDS             complement(2415..3383)
                     /locus_tag="ACG827_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0484766.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            complement(4550..4705)
                     /locus_tag="ACG827_002685"
     CDS             complement(4550..4705)
                     /locus_tag="ACG827_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484767.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4719..>5095)
                     /locus_tag="ACG827_002686"
     CDS             complement(4719..>5095)
                     /locus_tag="ACG827_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFQ0484768.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISIIYIR"
BASE COUNT         1598 a          839 c          840 g         1818 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacactatg ccaagtagcc cctctaccaa tggatagaga cgctcacttt
      841 gtgatttcta attggtctag cttaaacact tcagccaatg cttgatttta tgcacttttc
      901 ttttttcatt tctttgtgca ttttgaccgg tcatttttta gagatttgtt atttttgtga
      961 gcatttgata tgctcttttt atacctcaat ctcttctttt ctaatgttaa tgaataggat
     1021 aatctcattt ttagctgctc gtgagagcag tctttttttg tttcagggta cgtcctattc
     1081 tttggatttc cgcgctatta aataaccgat acttggggat tggggtagcg ccccaaaata
     1141 caaaaagaag aagttgcata agcggatttt tctttttttt cgcaagttgg taccaacttg
     1201 caaatcttgc tggaagactg atccctaaaa agaaatcgag gaattttttt agggatcggt
     1261 ttcttttttt atctttttag aaagtttgtt gggtcaaaat ttccagaagt aaaggtttga
     1321 ttggtttctt tttgacgatt tttgggttga tttcgtcggg aaggattgag ttgattttca
     1381 attttttctt tcacttcttg atacacttta gaatttgtac cgataagaac accagtatct
     1441 tttagatata aatcagcaaa ttctgccatg atttctgtca tgatagaaat ttcttttccc
     1501 atggcattga gttttgcttg gttactggtt gtgattgtat tggtatcagg ttcataactc
     1561 atacataaaa aacgaatggc ttctgaataa ttgttgacgt ctggattttt ttcgatgatg
     1621 gaatctaaaa cgtcaacgtg actgggataa agggttacgt gtttttgaac agaagttttt
     1681 ctcatgttat attcctccga taagtgtttc aatgagttgg ttgtttgtgt gaacatagtc
     1741 attcagtatg ttcgtatttt ctgtttgaat ggcaataaat ttttcgagta ataaatcgaa
     1801 ttgatctttt ttgtaattca taagttgacg gtcaaaatca ctttcgatta gaacttttag
     1861 atatttattt cgactccatt ttttgcccgt tttggttgtg agttcctgac aacgtttatc
     1921 tattcttgca ataattttgg gattgatatt tcgaactaaa atgtccatgg aaattttcct
     1981 ttcgtatcag cgtataattt atgtatatca tacaaatata gtatttgtat agctattttt
     2041 cgccaaaaca ctatacatag cctagccaca atggcaaaaa agtgatagca aatttttttc
     2101 tgagaaacgt gataggcaca gcctatctag caccttttgt aagaaagtga taccacgttt
     2161 ccttatgctc tttcaaaatt cagaaaaatc agctgtaaaa cgagtgcagt ccgagtaaga
     2221 ttcaacgaaa aaacggggat tggggcgcca ccaaaatatc gcaacatggg tccatgttgc
     2281 tctgctcgca aaagcaaaaa gaagaataaa accagcaatt taaattaata aaaattgctg
     2341 gttttattgc cagaattgcc accatttttt ctccgtcttt gattgttttt tctgtcataa
     2401 aatctaaaaa cccctcaatc tttgaataag ttttgaagat tgtctaataa gtcttttttt
     2461 agttctgaag gaatatcgga atttatctct gatttttgtg cattattttc tttattttgg
     2521 gtattttgtt ctctttctaa cttttctaac tgttgtaaaa gttccaattt tcgcttttgt
     2581 tctttgattt tttcttgtaa tgaagtaggt gctttttcag attgaacttg ttggttagct
     2641 agttctaatg ctttgttttg ttcttctttt gttggggcat gtttgttaaa gtcgtctgat
     2701 ttttttatct caggtttaaa tgtaaattga tagccaatga caggctttcc ccgtcctttt
     2761 ccgtatttct ttttgattgt taaaccttta aagattgcag ttagttcttg tcgtattgga
     2821 ttgaacacac gttgatcaat cgtaccttgg ttgtaacttt taggaatatc aagtaattca
     2881 tgaaagtctt cttttgaaaa ataagcgaaa cctttggttc tgtattgttt tagtaatcga
     2941 aacattgttt ttgaataact gctttctaat tcattgaatt gttggagaga atagcgcacc
     3001 cattcttcaa gattattaag taagggtaag gctttttcat gaacttgtat ttcagcaaaa
     3061 ggaatatcag attttccgtc gattttaaat tgattaaata aaacaaaacg ttcacgttgc
     3121 aaaccgtcag cacttcttct accaaaacgt aaatccatta atttgtcata cgttgtttct
     3181 aaatcatcta tgaatcgaac atttgcagtt gctttataat tgcttaaatc ttttaattgc
     3241 tcgaatgtaa attgtacttt tttatcacct acatcacgca ttcttgatac gatagaaaag
     3301 aacaaattca tttcgactgg tgtaaatttt cgcaacggaa ttgtatttaa ttcatgatga
     3361 tactttacga tttcattaga catatttaaa atagctcctt tcttactcgc acaagagata
     3421 cacgttatga aatacaaaat gcctatttat ctacatctta tattaacatc gtcgatgtac
     3481 taattcaatg tacaaacaga caaaacgatg tagataaaca gacaaaacga tgtagataaa
     3541 cagacaaaac gatgtagata aacagacaaa acgatgtaga taaaagcgtc attcccttgg
     3601 gagagtaagg atatagccga cggtaaagag gtttaaaaag aggtttaaaa agagattata
     3661 aaagaggggg acaaaatttc ccgcctaaaa aaccacttct tttttctcca aacagattcg
     3721 cgcatttttc tcaactggga atcttactcc ctacagtcgc ttaaaaaggt gtgatcgctc
     3781 tgctctcaca ttatactgcg cagtcgcttc cggctccttt gctaaaaata ttcataagga
     3841 actctttgag aagctatgag ccttcgtttg agacgtttta agacagtctt agtataaatc
     3901 ccttatcttg ttcttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaag
     3961 ttgtctgaat gccctctaaa attcgattta agcactctta aacctattct tatatattta
     4021 tatacttaga cgtttcaaac gtcttaaaac gcaaattaga gcttgataaa gatatttgct
     4081 cgtgtgccga cttttttaaa agtctaggca cacgccatga caaagctgaa ataaactgct
     4141 tcagctttgt catggcggat tcgctcactt gaaaaagtgt tcatttcgct cgattattag
     4201 catatatttt ttaacttttt ttgctagcca aattttgcat tagttcgctc ataaatccca
     4261 ccctttcagt acgtcctacg gacgtatcgt tcgtaatgtg aaagtacctt taaaggtact
     4321 ttctacaaaa ctcactaaag ggtttggcaa gctctatatt tgctagccaa atacctacgt
     4381 atttggtgca aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat cccccctcaa
     4441 aacccacgct ccacaataca aaaaaggcta gcaaatgcta gccacttttt gtaaagtttc
     4501 gcttgtttca ccttcggtga aattgttgct ttgcaacgat caggtagagc tacatttttt
     4561 ttcgttgttc aaatattctt acgccattta ctattaaaat cgtaggtatt ccaaagtaaa
     4621 gaaaaaattg attagcaggt aatacaaaat ataaaataat accagcaata attaccaaac
     4681 ttatagtaag ttttgttaat ttcatgaagt atgccacctt atcttatata gattatagat
     4741 atcaaataga ttaatatgcc gattgtatta taaaatccat gagcaactat ggaataccat
     4801 atattttttg tttgtatata aacaaaactt aaaataatac caataaagaa aatacttagg
     4861 cctcccccat gaacaataga aaacagaaaa gaagaaatta gtattgaaat atattgttgt
     4921 tttttgtttg cttgaggatc aatcatacaa aatcgaaaaa ttccttcttc taaaaaagct
     4981 ccataagtag cagcatttaa aaagaataaa aggagagaac catttacttc attaggattt
     5041 ataccttctg aaaaagatgt atttagaata ttgattagga ataaaacaga gaatg
//
LOCUS       DAZKOU010000128         4933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000128 DAZKOU010000000
VERSION     DAZKOU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.133"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG827_002687"
     CDS             85..972
                     /locus_tag="ACG827_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0484769.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG827_002688"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG827_002688"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0484770.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG827_002689"
     CDS             2274..2720
                     /locus_tag="ACG827_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0484771.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG827_002690"
     CDS             2993..4345
                     /locus_tag="ACG827_002690"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0484772.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG827_002691"
     CDS             4516..4803
                     /locus_tag="ACG827_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0484773.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAZKOU010000129         4902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000129 DAZKOU010000000
VERSION     DAZKOU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.134"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            434..1474
                     /locus_tag="ACG827_002692"
     CDS             434..1474
                     /locus_tag="ACG827_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0484774.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2298..2630
                     /gene="traE"
                     /locus_tag="ACG827_002693"
     CDS             2298..2630
                     /gene="traE"
                     /locus_tag="ACG827_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0484775.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3249..3590
                     /locus_tag="ACG827_002694"
     CDS             3249..3590
                     /locus_tag="ACG827_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0484776.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(3827..>4902)
                     /locus_tag="ACG827_002695"
     CDS             complement(3827..>4902)
                     /locus_tag="ACG827_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0484777.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1696 a          695 c          918 g         1593 t
ORIGIN      
        1 tacttattgt attttttagt agtatataat ctattgtttt ctagcctaaa gccttgtctt
       61 ataagaataa tataatatat actactgaaa taaggtagta tatattacta gtaatggtag
      121 tatgttttat atgcataagg tagttcttaa tttatttttt gttttgacac tggtagtact
      181 taattatctt taattttaaa actaaatttt attcatacgt ttttctggta gtatttaatt
      241 ataaataata tttatttaaa gtagttctta atgactttaa aggtagtaca taattcattt
      301 tatttctgat tttaggtagt atttaattaa aaaacagttt taatgcgttt gcatttttta
      361 tttatctttg ctattttcga aaagagtagg taattttaca taaataaaac aacgtgaaag
      421 tagaggatct acaatgactg attttaaatt ttttaaggcg gatagagttt acaacgaatt
      481 attttatcaa tttccaaaag tctttattgt ttctgacgaa tacaaaaaaa tgaaagattc
      541 aactaagatt gcctatatgc ttttaaaagc aagattagag atcgcaatca gcaaacggca
      601 aatcgatgaa gaaggtaatg tttattttac ttatacgaca aatgaactat gtagagtatt
      661 aaactgccaa aaacaaaaag cgatagcaat caaaaaagag ttggaatcct ttggtttatt
      721 attacaaaag cagatgggat ttaacaaaca gttagggaaa aataatccta atagactata
      781 tctagcagaa ttaaaagtct cagaaaatga tatctactta ctcgaaaaat ttgatagaga
      841 gaatagggaa aacgttgata aatcagaggg tatgaaaatc atacccaccc tcgacgaaaa
      901 atcagacgct gaatcccttg gggctcaaga gggtatgaaa atcataccgt gccaaaacgt
      961 tgataaatca gagggtatga aaatcatacc agaacttaat aataatatat tagacactaa
     1021 tagacacaat atagacactg aaaaagaccg cctacaagat caattgttgt tagacaattt
     1081 tgagacaatt atgacaaacg acagcattgc tacgtttgtc cctgaacgag tattaaattt
     1141 gataaaaaca ttttcttcaa gttacagtga agctcaaaaa accgtccaga ctattcataa
     1201 tgcaaagaaa aaagctgaaa tagaaagtga tatttcgata gtttttgaag aactcgatag
     1261 ttattatgtc aatgcagaac aagaattata cacgacactg ttaaaagcct atcaaaaatt
     1321 aaaaaccgaa aaagtcgaaa atatccagaa cctgattttt gtctatgtaa aaaattggtt
     1381 tatcgaaaaa ccaatagctg ctaaagtatc aagtgaaaaa cgtttgaatt atgcaagctc
     1441 cccaagcact attacgaaag actggttaga gtgaaacttt actgacaaag tgattgttgc
     1501 ttcatggagt aacattaaaa ttaaaaattt tttattattc taattatatt acgaatatta
     1561 caaaataata gctattttaa tagaatttat aagttaaaat attatgataa aaatcacaaa
     1621 cgtagccttt tacgtcataa gtgttaaaag aaataattaa tttcaaatat agaaaaggga
     1681 acgtatgttt gcatactcct tttttttgag ttataataat acgcgaagag agataatttc
     1741 attatttaaa caagctaaaa atcttgatat tacttctttt caggcaatac gaatattatt
     1801 cgcttacatt ttttgtataa atttaattgt ctgctatgct tgtgatgaat tgattttcgt
     1861 tttgcacttt tggatgataa atttggacaa ttgaaaatac aaaaaaaaga aaggcggtat
     1921 gaactcggaa ggctggtaac cttcgtcagc gttcatacac ggcattaaga tttggccctt
     1981 aatgcgttaa cctttcagtg ttacggtatt acccgcaatg gatttagtat aacagatatt
     2041 caaaagtgat accacttttt ttggctgtgt taaggttaaa aatacgtgat attacgatga
     2101 atctatgcct aagcataatg ttataaaaat gtccgtgtgg ctctttgccg tatgacgaac
     2161 gctgaaatta gagggaacgc atgtttggat gcgttccctc ttttttgttt gtcatgaacg
     2221 aaaatcaatt ctctattttt gaaaaataac aagaaaaact tgttaaaaat atttaaagaa
     2281 attgggtggt ttattttatg tcatatacag gaattttgag tctcaaagat atttgtcact
     2341 acggtaaacg gtgtaccgct acagagaaaa tcacgaagaa actatcaact ggccaaagta
     2401 aaaccgttgt gcagtgtaag aaatatataa tccagaaaga caaagtctca gaggaaatga
     2461 tttattacat tgggaaacaa aagcaaataa ttttaaaaga tcctatcccc ttaaaagagc
     2521 tatatccaac aatcaaacat gtttatgacc aaaacggtgt actgattggt cgaagaaaaa
     2581 atggagtctt gcgttgtacc gcaaaaggga tggggcgttt gatcggctga attgatcatt
     2641 ctcataactt gtattttatc gtttttcgct tgcttgaaaa tattgtcgag acttctaaag
     2701 gaacttagag tcactagaaa aataaggagt agacttgatc tatcaacaat tcccttcggg
     2761 gagtgacggg gtaggtttta acgattggta acgtaaaaaa ttggtcaatc gagtaaatgc
     2821 gcttgtttgg atagtggtga aatgaatttg tgtaaataat aagcgcagag taaacaagag
     2881 ggaatttaga agttagacaa gttagaatag cgtctagatt aatcatgagc agctactaag
     2941 ggatctagac tttgacgcaa actcatggaa taaggagtag gtcttctcgt aatggggagg
     3001 acgcacaaga aattgtgtcg tgtgctgcag tacatgtaga tacttgcgag gacgttgatt
     3061 attggatcaa tcgagcattt accgctgtct actagggttt ctcgtttcgg acgccataaa
     3121 cgagtcgaaa ttttttatgt atttttaagt aaacgaaaaa cgataaaata tgaacaagtc
     3181 ctgaataaag tgaaaaaatt aatataaagc atagagaaaa tgaaatttac gtaatggagg
     3241 aatgacaaat gaaaaattat gatccgaata tacgattggg cacacatact attaaggttt
     3301 ctttccaacg ttgggattac aaaggatttg tgacgtttag aaggggcgga aattgtaaag
     3361 gattagatgt tttagcatta gacgaggatg atttatacga tcaaacattg actgataatc
     3421 caattggatt cggcttgcta cctgaagatg atgaaggtaa tgaatggttt aaaatgacgc
     3481 ttatgaatga taatggagac gaattatctg ttgaagatac ttggtcatat ttgagtgatt
     3541 atattgtcag ctttgaaatc attgaatttg tagcggataa agaagaataa aaactggagg
     3601 aataaatttt acagataaag gcttagaagg agtcacgacg attaataaaa aggtagtatt
     3661 accttaggca ttgagtaagg aatttattta gtctaatggt gataagtcaa gatgaaattt
     3721 aagattttct ttattgagta atatgttttg aagcgcacga aactagaccc gatcaaaatc
     3781 aaattttttt cgggttgatt caaggatttg cgtcaataac ggattgttat ttctctatca
     3841 cactccttgg tggcgtatag agttggtgtt tacgtagtag cgtatccacc agacgcgtaa
     3901 attttcttgc ggttaggacg agtgcacgtt tgtgttgatg ctttggaacc tccttatatt
     3961 tactttgata aaaagctttg tattcaggaa gataattttt tacagagttg gcagcttcaa
     4021 ctaagtagta tctaaaatat cgatttcctt gttttgtcag aggtgtattt tgcgactgat
     4081 agtttccaga ttggttcact ttccaactta atccagcgta ttttgccaat tttgtctgat
     4141 cttcaaatct ttcgatttga ccaatttcag cgattaaacc ggcagcatag actttaccaa
     4201 cgcctggaat actggttaag cattgatatt ctggaatgac aacaattact tgttcgatgg
     4261 ctttatctaa ttctttgata tttttttcaa gtgctcgtat ttctcgtacg agaacactta
     4321 aaacaacatt aattgattct tgtgcaagag cacctaggcg gtaactcatg gtaattgcac
     4381 gttgaattgc tttagcgatt ttttctggtt gcttgaaacg gccttttcct ttttcttgaa
     4441 gtaaatcaca gaaagcttct aaaggaagct cagcaagttc atccaatgtg aaatcttccg
     4501 tcatgagtga aatgagggtt gcactaaaaa gactcgtcga ttcgtctcgc atttctcgag
     4561 caagcgtatt acatttgtac gttagatttt caagaaaatg ttgctttgta cgaatcaatt
     4621 gcttaataag ttgatagcga gtacgtgtta aacgctgtaa ggccatgtat ttctcttctt
     4681 tgatagggga agttgtgaat cgttgaatac gcaagaaatc agcgattctt aatgcatcgt
     4741 ttcgatcagt tttattctca tcaaacatgc gactaaattg tttcacccga aaaggatttt
     4801 caatcgttac aagtgtgttg agcttcttta gtttctcatc ttcatgaaaa aacatggaag
     4861 gatggaagct gtacatggac gttgactcca tgccaatcac aa
//
LOCUS       DAZKOU010000130         4892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000130 DAZKOU010000000
VERSION     DAZKOU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.135"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACG827_002696"
     CDS             complement(287..649)
                     /locus_tag="ACG827_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484778.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACG827_002697"
     CDS             complement(723..1103)
                     /locus_tag="ACG827_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0484779.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACG827_002698"
     CDS             1185..2360
                     /locus_tag="ACG827_002698"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0484780.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACG827_002699"
     CDS             complement(2437..3021)
                     /locus_tag="ACG827_002699"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0484781.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACG827_002700"
     CDS             3222..4220
                     /locus_tag="ACG827_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0484782.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACG827_002701"
     CDS             4332..4841
                     /locus_tag="ACG827_002701"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0484783.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1553 a          914 c          851 g         1574 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt tt
//
LOCUS       DAZKOU010000131         4757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000131 DAZKOU010000000
VERSION     DAZKOU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.137"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACG827_002702"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACG827_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0484784.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACG827_002703"
     CDS             724..1878
                     /locus_tag="ACG827_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0484785.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACG827_002704"
     CDS             2093..3061
                     /locus_tag="ACG827_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0484786.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACG827_002705"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACG827_002705"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0484787.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACG827_002706"
     CDS             4091..4699
                     /locus_tag="ACG827_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0484788.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1471 a          910 c         1115 g         1261 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaa
//
LOCUS       DAZKOU010000132         4754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000132 DAZKOU010000000
VERSION     DAZKOU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.138"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..1098)
                     /locus_tag="ACG827_002707"
     CDS             complement(136..1098)
                     /locus_tag="ACG827_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0484789.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="ACG827_002708"
     CDS             complement(1100..2539)
                     /locus_tag="ACG827_002708"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0484790.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="ACG827_002709"
     CDS             2724..4670
                     /locus_tag="ACG827_002709"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0484791.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1512 a          813 c          779 g         1650 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttat
//
LOCUS       DAZKOU010000133         4714 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000133 DAZKOU010000000
VERSION     DAZKOU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.139"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(213..443)
                     /locus_tag="ACG827_002710"
     CDS             complement(213..443)
                     /locus_tag="ACG827_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0484792.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(673..1491)
                     /locus_tag="ACG827_002711"
     CDS             complement(673..1491)
                     /locus_tag="ACG827_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0484793.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="ACG827_002712"
     CDS             1652..2341
                     /locus_tag="ACG827_002712"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0484794.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="ACG827_002713"
     CDS             2355..3857
                     /locus_tag="ACG827_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0484795.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..4346
                     /locus_tag="ACG827_002714"
     CDS             3870..4346
                     /locus_tag="ACG827_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0484796.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4443..4553)
                     /locus_tag="ACG827_002715"
                     /pseudo
     CDS             complement(4443..4553)
                     /locus_tag="ACG827_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1592 a          673 c          829 g         1620 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttattatcca
     1621 aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4321 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4381 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4441 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     4501 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     4561 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     4621 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     4681 tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZKOU010000134         4660 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000134 DAZKOU010000000
VERSION     DAZKOU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.140"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..408
                     /locus_tag="ACG827_002716"
     CDS             19..408
                     /locus_tag="ACG827_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484797.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            412..1176
                     /locus_tag="ACG827_002717"
     CDS             412..1176
                     /locus_tag="ACG827_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0484798.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1305..2252
                     /locus_tag="ACG827_002718"
     CDS             1305..2252
                     /locus_tag="ACG827_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0484799.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            2382..3122
                     /locus_tag="ACG827_002719"
     CDS             2382..3122
                     /locus_tag="ACG827_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484800.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(3413..4081)
                     /locus_tag="ACG827_002720"
     CDS             complement(3413..4081)
                     /locus_tag="ACG827_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0484801.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4215..4502
                     /locus_tag="ACG827_002721"
     CDS             4215..4502
                     /locus_tag="ACG827_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484802.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT         1595 a          768 c          925 g         1372 t
ORIGIN      
        1 ataggttagg tgattaaaat gaaaacggtc aatcgtgtgt tttgtgccag ccacgtgcta
       61 agtctccttc tcagtatata cttaggtgtc tattcgattc aacagccaat tgaggtagag
      121 aacattgttt ttcagatgtc gctgacagat ggtttaattt taagcgtttt tttctttttg
      181 atactgttta ttggtaatat tttcggtagc gttgccagtt ttttgaattt ttctatttat
      241 ccactccttt cgctagcatt aggagtggtg ggactttgtt ctctgtgctt atttcccgag
      301 ccattcagtc ctgcatttat attatttggt acactgaacc tgttccagac aactgttgga
      361 gcatggttgc tatggcgatc aggaaattta atgaaaatag gtgagtaaca attgaatcca
      421 attatattga aacgttctgt ccatcatatt ccagtgttag aagttgttct gaaagaatta
      481 agagaggaac gtattcccgt tgtcatctat tatcacggat ggcagacaag taaggagtta
      541 gttttgacgc aaggaagaaa aattgcgcag cagggcattc gtgtgatcct gccagatgcg
      601 atgaatcatg gggagcgcaa acagccggtt tcttctattc catcctatac attttgggat
      661 agtatctacg gaaatctgtt tgaatttgac acacttatca agcatttaga aaaaagaggt
      721 cttctacatg atcagcttgc agttggcggg gtttcgatgg ggggaatgac aacatgcgcc
      781 ttgttggcta aacatcctca tatagtagcg ggcgtgtgtt tgatgggttc ccctgcacct
      841 ttggaatatg gaaaagaaat cgtaaggcgc gcatcggaat ataattatgt cgtaccaaaa
      901 gactattttg atttgatttc atgggttaag agctatgatt tgtcattgca gccggaaaag
      961 ttagctggac gtcctctgtt cttttggcat ggacaagctg acgaaaaaat cccttatata
     1021 caaacggaac gatttgtaga aaaaaatcgg cataagtctt acggaaaaga aattgttttt
     1081 cgttcctcaa aaacacagcg acatatggta caaataccac taatggaaga agcttccact
     1141 tttttagcaa aaaatttgat aaaaaagtat aaataattga aacggaaaat cactcatgac
     1201 gatagaggat tttccgtttt ttattttggg ctataataaa ctctgttagg aaaatttggt
     1261 ataattgata gtaagaatga caacatgtta gaaaggaaga gagcatgccc actttagcag
     1321 atgttgcaaa acgtgccaat gtttcaaaaa tgacggtctc tcgagtcatc aatcatccgg
     1381 aacaggtgac tgacgaattg aaagagttag ttttctcagc aatggctgaa ctggattacc
     1441 gtccaaatat tgctgcaaaa gcacttgtca cgaatcggtc gcagatcatc aaattattta
     1501 tactggaaga aatcgacaca actgagccct attacatgaa tttgctgatg ggaatcgcta
     1561 aacggatcgg taaagcgcat tattctctcc aattagttac aaatgatgcc ttcgacgtcg
     1621 gttcttgtga tggatacatc atcacaggtg tcagaaaaaa agacttcgat tggatcgacc
     1681 gtttggaaaa accagttgtc ttgtttgggg aaaattctga aggatatgat tatgtagaca
     1741 gcgataatga atatggtaca gcaaaagcaa cgcaatatgc cttagaaaga gattatcaaa
     1801 cgattattta tatcggtatc gaagtggacg agccttttga aatttcaagg gaaactggtt
     1861 atttatctgt gatgaaggaa gttgatcttc ctagtaaaat cttgcggttt ccaaatcatt
     1921 cgacagaagt agaaagatat atgacagaaa actgggcaga ttttacagat aaaacttgtt
     1981 ttgtatgtag ttcagatcgg ctagcactag gagcagtaag agggattata aatgctggag
     2041 gacacttgcc cgaagaattt ggtgtgattg gatttgatgg ggtattttta gaccaaatca
     2101 gttcacctaa attgacaacg atcaagcaag atattatacg tatgggtgaa gtatgtggag
     2161 aaatgttgtt aaaaaaaata gaagaaaaag gggcgagtca aggttatcgt cgtttttttc
     2221 ctgaactagt tatcagagag acaacgaatt aagtcttatt actatatata tatttatgaa
     2281 attcatcgct attttcttaa aataatcagc gcattgctac tgaaaacaat gaatctgtgc
     2341 tattattgag aaaagttcca tttttgagaa ggatggtttg aatgaaagaa atcagacgaa
     2401 aacatttccg ttttcctgcg tacgatgatg agttaggcgt aaaattaacc aacgacaaga
     2461 agcgagtatt atttgacgga caagaagatc ttttgactga acagaatcag tccccgtttg
     2521 aacagatgaa agaatttcat ttcacgaaca cgaacaccga gaaaatccgt cgcaagcgtc
     2581 gtgcaacaaa tggattgaag tcagctccgc gtaaggaaaa tttggaaaag cataaaggaa
     2641 aattaccgga ttataccggc cattaccaat cagaaatcac atcgacagga cgaaaaaaac
     2701 tttttggtga ttcctctcgt tctgtcagac aaagtgaaac aaggacagag tccaagcata
     2761 ctacacgaaa cgcatctgtt gacacgaagg tgcgggagcg ttcttatttt gttcctaaat
     2821 atgtacccgc atcgattatc ccagacccta gagaaagcaa aatttctgag gatcatttaa
     2881 tcgaatcgat gaaaaaaacg gatgactctt atctgttgtt cgatacagaa ccgacgccgt
     2941 atcaagaaaa aagaaatggt gagccgtctg tacgaacatt caatcgaaca aaaacgttag
     3001 aggaaacaaa gcagtcagaa gaaaaaaaat cgaaaaaaag ccatggtatc ttagaacgct
     3061 cattacaagg attgatcgat gatcaaggaa actctttggg agaaaatagc tattttaaat
     3121 aagtgatata caaatttata ttcggctaca gttggaaagg aaacgctgcg gcgtttcctt
     3181 attttatact ctatccgaaa gtttatatcg tatcctcaaa tttttttgag tttaagcgaa
     3241 ttttttctcc caataagtaa gaaaataatg gcaaaataaa aaaacaaact attttcgcag
     3301 atcgtttgca aaaatagttt atttttttat aaagaagatt ttactgaata atatcatttt
     3361 agtttacata atgatatttc tgtaaattaa ataaagtagt gaaaaataag aatcagtgct
     3421 ctaactgttc aagaggatca agtggttcaa atgtttctgt agtctgtttt gcttccaatt
     3481 ggggcaaaac agatccaatt tccagatact tcactttatt caatgtataa gatagaatag
     3541 aacagctaag taatgtatac agaaaatgtg tgagatccaa ataaagcaag gagacaacta
     3601 aaataccaaa agtaatcaag aaaagacttt tgccaatctt aatatgaaaa tatttttcaa
     3661 tggcttgata aatgatatct gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc
     3721 aaccaatgcc agaaagaata cctgtcatca aactattgat cagtagacta tctacagcaa
     3781 aatgtatatg aaggctctcc cataaatgta aaaagaaaac tagcgcaaat gttccgacga
     3841 tcgtccggag aaaagcgtgt tttccaaaaa gaaacaatga gataagcaat aaaggtaaat
     3901 taaaaagcag tgaagtgaca gaaggagcga atccaaaaag attatgcaaa agcaaagcaa
     3961 taccagtaac cccaccatct gccagcttag atggaatatt gattcctgtc acagctaatc
     4021 cgtagattcc tgcgcctaaa atgattccgc acaattctgt aaataaattt aattttttca
     4081 tttctagcct cttttctttt gaaaattttc agcaacttct ttattatact gacttatact
     4141 cttacttcaa gaggaaaatg aaaaaaaatt atttttggct gtttcttttt atttatcaaa
     4201 taaggagtgg aaaaatgcga gcagcagact tattgacaat tcttacaaaa tcacccaaaa
     4261 aaacagagta tcaaggagtt tttttggctc aagaacaaaa actggtgcct gtgacaatgg
     4321 tccagataaa taaagcaaat caatttatct ttttatttga acacggcaaa gcaccacttg
     4381 ctatgaaaga catctttctt tttttgatga agaaccgtga gaaagctgtt tattatcagt
     4441 caaaagaaga actcattcct ctttatggag tgaaagagat agaaaataaa gtggtgatat
     4501 aagtaaaaat aagaaaacaa gacgtttatg atgtcaaaac aaccattttt tacatttttg
     4561 gcaaaaaagt gtcatgatac agtataatat tcgttgaaca ggccaataaa ggtgttctcc
     4621 cccacgagat ttctacttta ttggctgttt ctttttatta
//
LOCUS       DAZKOU010000135         4541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000135 DAZKOU010000000
VERSION     DAZKOU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.142"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /locus_tag="ACG827_002722"
     CDS             complement(<1..302)
                     /locus_tag="ACG827_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0484803.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYV"
     gene            395..744
                     /locus_tag="ACG827_002723"
                     /pseudo
     CDS             395..744
                     /locus_tag="ACG827_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1016..1306)
                     /locus_tag="ACG827_002724"
     CDS             complement(1016..1306)
                     /locus_tag="ACG827_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484804.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1708..1944
                     /locus_tag="ACG827_002725"
     CDS             1708..1944
                     /locus_tag="ACG827_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484805.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1951..3903
                     /locus_tag="ACG827_002726"
     CDS             1951..3903
                     /locus_tag="ACG827_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0484806.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="ACG827_002727"
     CDS             complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="ACG827_002727"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0484807.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1555 a          798 c          750 g         1438 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt taagtagttc agttttggag tacaaaaagt
     1501 gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat ttgatactcg ttttttattg
     1561 ttaaatcaac gtttgttgtt aatttacacc acaaaatgta agttaaaatg tatataaaaa
     1621 tgcgaacgtg aaataattat tgtaggaatg caaattggtg tgttaaaatg gttacaattt
     1681 actaagcaaa aaggggtgca ttttcctatg caatttcttg agccaaaaaa taagaacgct
     1741 aagtctgtgg attgggaaat atcagagcaa gtaagggtga tagttaagca atacgctgaa
     1801 tatgcagagc gtactgaaag cgaagcagtg gatgaattct tattgaacat tttagacgat
     1861 aagaagttta ttgaatggat tgccaacaag cgaagtaata agcggattgt tgaaaaaatg
     1921 ggtatcaaag atagagtggg ttgatagaca atgaccaagc ttaaaagatt agccacgaag
     1981 gaagatgaaa ttgtagatgt taaaattccg atttcaaacg aagaattaaa agatagggct
     2041 aaacaatatg atttattaac tccaaaacga tttgcaacaa ggtataacaa aatgttgttc
     2101 ttgccaacgt cttttaaatg gaacggttct gaatatccca ttcaatataa ttactgcatc
     2161 aatccgtttt gttgtaattt tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa
     2221 ccaagtcgat ataaaatgac tggtagttcc aaagataaag gtcataaagg tatgtactgc
     2281 aatgacaatc ctattggcag aggtgtatcg caaaactgta cagtaacccc tttatctaat
     2341 tggtctgttg ttgaagaaat taaacgcctt atagaaatca atagcatcca agatgttgag
     2401 ccagattatc aatttcataa agaaggctgc tcggaagaag aatcaacccc attcaatgag
     2461 ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag
     2521 gcgtgtaaga agttcactaa tgttctacca aaaagagaag aaaccactac ttatcaccaa
     2581 caaaagaaca ccattcttcc aatgttcgct aagatggttg ttggaagggt ttcagtcagt
     2641 cgtacttgtg atattttagg aattggagtt ggaacctact atcataagct ggagtggctg
     2701 tataggcgtt gtttggagtt tttagaacga tatgagacac aaccccttca aacaaagaaa
     2761 tttaacgaga tgtggcttaa tacggacaag atgcactact acctaaataa cgtccgtaag
     2821 aaaggacaag gttcaaagaa atatacgggg tttgaagacc ttaatatgca aacgtatata
     2881 gttgtatctg ctgaggtctt atcaagatat gtgttccgtt ctgatgttgc ctatgattgg
     2941 aatatttcaa tggatgaatt aaatgaagat acaagaaaat tcaaagaaga ccatctcaat
     3001 acttttagtc gtaaaaatga taggttagat tggtcttatt atccacaaga accatccgct
     3061 aacgatagcg aaaataggaa tgcttatctt cacgaattag ggaaaattac taataggagt
     3121 agatttgtcg atggtttaaa tgttgatgct ccttatacta caactgctca ctattggtta
     3181 attaaacaaa tggttaatgc cgacgaatgg cgaatgatta gtgatgatga cttctctatt
     3241 agaaacgcct tttatagagt attcacgaaa gaattaaggt tgagcgatgc acatcatttt
     3301 atttgccaag ttaacaagac gaaaagcaga aaacaatgct taaaagaatt cggacaagca
     3361 aaagcagaac tgctcgattg gggagatatc agaggattca aaacaaagtt tctacggact
     3421 ttggcatctc attatctaac tgaattacta acaagtcatc aattccacga agaggctatt
     3481 agtaaagatg gagaaagata tcgcaaatat gccgataacc ctatcaaaca tccattagcc
     3541 actaaggata aaggatttta ttcagttgat tgtagaacgg atttatctgc tctggaacca
     3601 aacgaaatcg caaagatgct attaaatgtt aatgaccatt cgaccaatag ctttattcaa
     3661 cagataagac gttatatatc atctcttgaa agaccattaa caacagctcg tggtgataag
     3721 aagagctata tttacgctaa cttcaatccg aagtatgctc aatttgcgat tacaattcta
     3781 agaacttact acaacttttg tcgccctttt aaatcagcgg ataaaaaggt tttgacacct
     3841 gctcaaagat tagggattac agataaacaa tttgattgga aagatataat ttatttcaaa
     3901 tgacaaaaag agcttggagt aatcccaagc tcttgtgctt tatctttact gttcttcttc
     3961 aattaacgca cccgttaact caataagttt tttctttcat atacatgaaa atagtagtta
     4021 tacggatttt tatcattctt tatccctttt tgggcaaata cctcattcca ttcctcataa
     4081 ttcacttctg gaaaaaaagt atctccttcg aattcatgat gtatttttgt gatgtacatt
     4141 ttctcaacat aagggaaaaa caaattataa atctgttctc ctccgaaaat aaaaatttct
     4201 tcttcgtttt tacataactc aaaaacatct tctattgaat gaacaatttc acaaccatta
     4261 aaggtaaacc ccttatctct cgtcagaata atatttcttc tgtcaggtaa ggctcttccg
     4321 attgattcaa ggttcttcct acctaatatt atcggatgtc cctttgtagt atttttaaca
     4381 tattcccagt ccttgggaat cctccaagga atgtcattct ctttgccaat cactctattc
     4441 ttatccatcg cagcaatcaa agaaactttc atttataaca cagccttttt aactaattta
     4501 ccatctaatt tcttatttct atcaaaaaac cttttctcat t
//
LOCUS       DAZKOU010000136         4482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000136 DAZKOU010000000
VERSION     DAZKOU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.143"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..347
                     /locus_tag="ACG827_002728"
     CDS             <1..347
                     /locus_tag="ACG827_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484808.1"
                     /translation="FDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCRTCIQKAV
                     RYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQSIIGVKTL
                     AYLSGMGRNRRF"
     gene            665..1255
                     /locus_tag="ACG827_002729"
     CDS             665..1255
                     /locus_tag="ACG827_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFQ0484809.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            1282..2592
                     /locus_tag="ACG827_002730"
     CDS             1282..2592
                     /locus_tag="ACG827_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0484810.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            2589..3308
                     /locus_tag="ACG827_002731"
     CDS             2589..3308
                     /locus_tag="ACG827_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFQ0484811.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            3323..4408
                     /locus_tag="ACG827_002732"
     CDS             3323..4408
                     /locus_tag="ACG827_002732"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0484812.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
BASE COUNT         1598 a          493 c          867 g         1524 t
ORIGIN      
        1 agttcgattg taatattgaa ttagttaaaa aaattgaagg cgatttaagc tatatattag
       61 aaaatgagat atttgtaaac aaaacaagga gacttttgtt gggacgctgt cgtacttgta
      121 ttcaaaaggc agttagatat gctagtttta ataaagaatt tgatttaaac tcctatttgt
      181 ctaagatact tatgaacgat gagttgattg cctgtattca ctcataccca tataaacaaa
      241 atccaattaa acaacgaata tttaactatt ttttactaaa ccaatccatt attggggtta
      301 aaacattagc atatttaagc ggaatgggta gaaataggag attttaatca taaaaaactg
      361 tactatagtg aatttttgga gatttatatt ccagttatat attaatgatt gtgatacttg
      421 tactattgta aaaattcgtt aggtttaaat aactggaaat atcggaaaat gggcattata
      481 catcacggac atcccttctt ttggaaaaaa tgttacgatt tttataagta atattgtatc
      541 cggtggaatc aacgatgaag ataacatatc catcgttgta gattcagtag cattgaatga
      601 tgctcttgtt gcaagaaatc cgaataaaat tgttaagtat aaattgaatt aataggagat
      661 atgaatggaa attagcagta aagaatattt aaaagcggtt cttcgtatgg aaaagaatct
      721 atatatagaa tctaaaggac aacacacaaa attactaaaa ataatgaaat cacatccgga
      781 ctacaacgct tggcggtata ctcgtcgaat gagaattaca tcttattatt attcaaagag
      841 aaagaaaaat atcatttatg cgattatgta tattgtgtct tgcagaagaa tgaatagttt
      901 gggaagaaaa tgtggtatag agtgtggtga aaatgtattt gatgaaggtg taagatttta
      961 ccatacacaa ggaattgtta ttaatggtaa cgccataata ggaaaaaatt gttacttata
     1021 tgggaataac tgtattggaa atgatggaat cgatcctaag tgtcctgtaa ttggaaataa
     1081 tgtacgtgta tgcgtgggtg caaagattat tggtaatgta aaaatagcta acaatgtagt
     1141 tgttgcggca ggagctattg ttatcaaaga ttgtctagag aatggtgcaa ttcttgcagg
     1201 tgtaccggca aaaataatag ggtatgttaa tttaacaaaa gtatttttag aataattttt
     1261 agcgaaagaa ggtgaataac gatgcgtatt tcaataaaaa gatggtcagg agcagattgg
     1321 acattgttca cgttggcatt ttttgcaatc tccatagttc ttgtatatac ttttaacgcc
     1381 ccaagagcaa ttttatattt gggagatata attaatatct ttgtgttatt aaatgcactt
     1441 agctttagac gcagaaaagg aatatatgct agcaaaaatg agatgctgat agttttgttt
     1501 ttactagttt ctatttgtag ctcagttatt aatttagaaa atataggatt gattatctgg
     1561 gggatacgaa ataacactcg tatgttcata gtatatataa attgtgcaac atatttaaca
     1621 attaaagatt actataaagt attcgctata attgaaaaat tattctggat ttcactgcca
     1681 ctttgtttaa ttgaaagatt catggtcacc tatccaactg gaacaattgt aggagatatg
     1741 ataggtggag ttttttggaa ttattcaggt tgtaatatgc cattgaattt aattttgtgt
     1801 gtgacagtta ctgttgttac aatcggatat ttcaagggag aaaaaaattt catccatttt
     1861 attttatgtg tttgtgcagc actttttatg tctgcattag ctgaattgaa agtttttatt
     1921 cctgaattat ttataatatt tgttgcaata gtattcactt taaaagttga ttttaaagta
     1981 gtgatcaaga tattatcatg cgtaatactt gtgtcgatag tattatcctt ttttataaca
     2041 atatttatta cattgaacga taatggacaa agctatgctg ataatttcac aattcaaggt
     2101 cttttagaat atgcgtcaag agattctgga tatgatggtg ttggtgattt aaatcgaata
     2161 actggtatct ctagagtagt agatgaaata tttaataata attttagatt agtcctaatg
     2221 ggagtaggca ttggcaatgc agagtttacc aatttttttg tcagtgagtt ttataaatcg
     2281 tttggtaatc ttcattatca gtattttcat catatttggt tatttataga agttggaata
     2341 ttaggagttt cgatattcct attttttata ataaagacat ttttcaatgc aaaaagatct
     2401 tttatgaagg ataaagatgc aagtcttgtt aaagttattg caattatgtc ggtattcttt
     2461 tttgtgtata acacaacact taggggtgaa acaacggggc cactgattta tatgatttta
     2521 gccatgcctg aagttgtgaa aaggaaagag cattattata gtgaaaaaaa tacaaaagga
     2581 gagatattat gattcctaag aagatacatt actgttggtt cgggggaaat ccacttccga
     2641 aaaaagatag aaagtgcata gaaagctgga aaaagtattg ccctgattat gagattattg
     2701 aatggaatga atcaaattat gatttaaata agaaccgtta tatggccgat gcatataaag
     2761 aacgtaaatg gggttttgtt ccagattatg caagatttga tattatttat aatcatggtg
     2821 gattttattt agatacagat gttgagctaa ttaagtcgtt agatatactt cgtgaaaatc
     2881 atgcctatat gggatttgaa ggggaaatct gggttaatgg cggtattggt tttggtgcag
     2941 aagaaggaca ttctgttatt aaaggtttaa gggatattta cgattcatta agtttttatc
     3001 aaaatgatgg tacattgaac ttgaaaccga gtccgtatta tattacagat tatcttgaat
     3061 ctattggatt ggttcgcgat aacagcgaac aaataataaa tggcctgcat atctatccta
     3121 cggattattt tgctgccaag gattatgata ctggaaaaat ttataaaact agtaatacaa
     3181 taagcattca tcaatataat gcatcttgga aaacacctaa acaaaaattt gtttatagat
     3241 taaaatcaac aattggacaa aaaaaattta acaggttact tgcactgaaa aaatttttac
     3301 acttataaag gagaaaagta ctttgaagaa aatttgtttc attagtggtg taatttctcg
     3361 ttctggggga actgaaagag tagggagtat tattgccaat agcttgtctg atttaggtta
     3421 tgatgtcact attttaagct tttggaatag tggagagcct tattttgggt tagataagac
     3481 gattaaggtt gattatatgt taaatcctaa aacggaggga aaattatata gaacttattt
     3541 atatccggtt tggaaattgc acaagtatat atgcaaaaat gaatttgata tagtaattga
     3601 tattgatact ttgttgtctt tgtatactac aagggcaatt aaaggaacaa agtgcaaatt
     3661 gatttcttgg gaacatttta attactggac aatggaaatg ctgaaagaaa agaagagatt
     3721 caaggctaaa aaattgataa aaaaatatgc aaagaggctt gtggttctca cagaagaaga
     3781 tcgtcaaaaa catattgatg aatatagttt atcagaagat tttgttataa ctatgccaaa
     3841 tccgtgtata tctaatatcg agtacgaata tcattatgat aaaaagagat ttcttgcagt
     3901 agggcgatta gtggaagaga aaaattttgc tgacatgata agtgcttgga aaattgtaga
     3961 aaaaaaagta gatgattggg atcttatcat tgtaggaaaa ggtgaattag aaaaagatct
     4021 tcgccagcag ataaaagatt taaatctgca aagagtaact atagcagggc attcagacca
     4081 tgtggaggaa tattataaat ctgcatcttg ttatgtgctt agttctgttt atgaaggatt
     4141 tcctatggtt atattagaag cacagtctta tggattacca gttatttctt atgattgtaa
     4201 aactggtccg agagatttgg tacatcataa ttttaatggg atgttggttg aagataagaa
     4261 tatagaccaa ttagctaaga gcatgataat gtttactaag aatactgatt tagcaatgag
     4321 aatgtcactg aacgcatata ataatgtaca aaaatttaat ttgaaagaaa ttacaaaaca
     4381 atgggttgct ttgattgaaa atgtatagtt ttttgtggtt gtccaaacat gaaaattagc
     4441 gtaattaatc aaagagctag aagaaaccta ttacatttca ag
//
LOCUS       DAZKOU010000137         4466 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000137 DAZKOU010000000
VERSION     DAZKOU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.144"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..884)
                     /locus_tag="ACG827_002733"
     CDS             complement(459..884)
                     /locus_tag="ACG827_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484813.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1258..2229
                     /locus_tag="ACG827_002734"
     CDS             1258..2229
                     /locus_tag="ACG827_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0484814.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2261..3658
                     /locus_tag="ACG827_002735"
     CDS             2261..3658
                     /locus_tag="ACG827_002735"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0484815.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3817..4440
                     /locus_tag="ACG827_002736"
     CDS             3817..4440
                     /locus_tag="ACG827_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484816.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1562 a          717 c          911 g         1276 t
ORIGIN      
        1 aattactacg tgatgtagaa taagcctttc tatcgtttgg ttattttata ttcaaatatt
       61 tgaaataaat aaatctttac tctttgattt gtaaactgaa atgatccact gtttgtttta
      121 ctgatgaagc ttgatcaaag gaagaagctg agtttgaagt tcataaaaat cagccatagt
      181 gagttttgct tggatttgtt tatcttcttc tactttttac ataattttga acaggataag
      241 attcaaatgt tttaaatgaa tctttgacta gtttgtgaca gttgtcgcat atgctcaagc
      301 ttccttggtc attaaattct gtatggatat ttttgttgtt attacatgta caataatcag
      361 gactatcgat catccatttc gtctccttta caatcatctt gcttctttac tatagcagaa
      421 aagaagggga gaaacaaaag aagatccaac agacggcact atagactgta gatgttgcca
      481 tcctcatcct tcaaacggta attaccgatt tttgtaaata tgactgttgt ttctttttta
      541 ttttcttttc ctgaaataat atattcgtca tctttggtag tgatcttgat atctgtcagc
      601 actgtttttt cttcagatga ttcagaagaa tcagttcctt gtgtctgata cgtgcgtgtc
      661 cattccattt ttttatctgt aaatgtcaac tgtcccgcgg ataaatccat atctttacgt
      721 acttttagcg tatatacact gttggtggta tctgcttttt ccgcttttaa gacagaagaa
      781 tcggttgttt cgtccgagga ttggtcagag ccgccaaaca aatcacagcc agtcaatgtc
      841 aaaagtcccg cagacaaaat aaatagtacg attaatttct tcaaaatact tcccccattt
      901 gataggataa agaaattata ccattggaat gtaaaacttc aaacggatgg aagagaaaaa
      961 cgcacaaaac acccacaaat agagtaaatt ttagaataaa attttaaaaa ggcaaaaaat
     1021 ccgccacttt cagaaagcgt tttaaaaaag tgttgcaatc ttttaaaaaa atgctatatt
     1081 aaaaatgaac cggttcaaat acttgtttaa gtataattaa tagtgagagg aagttgctct
     1141 actgaagact ttagttatat ggatattgat gacaagcaac actatgcaaa gaatgcggtc
     1201 atcataatcg aacaacaaga aagagaaatc aaatgtaact ggcaggagtg agaatgtatg
     1261 gcatcgatcc gtgatgtggc taagatggct ggtgtttcag tgggaacagt ttcccgttac
     1321 ttgaatggtc agcaactgaa ggaaaagaac atgaaaaaaa tagcagaagc gatttcagcg
     1381 ttggactata aagaaaacat cattgctaaa gggttgaaga ataatcgaag cttctctatt
     1441 ggacttttga taaacgggat ttccagccgt tttgggtcgg aagttgtctc agggatcgaa
     1501 agagtagctg aagaaaatgg gtatagctta ttattaagcg ggtttgccga tcagccagaa
     1561 aaaattgaac aaaaaattga atacctgatg aagcatgtga tcgatgggct gattgtcttc
     1621 ttatccgaag aagagtggtc tggatttgaa atgctcacaa aaatgagtat tccagttatc
     1681 gcattgaatt gtccagaagg accagcaggt attgatacaa tattagtgaa tgatcgagaa
     1741 agtgtagcaa aagtgatcgc tcatcatggt gaacaaggac ataaaaaaat cggatttatt
     1801 gctgccactc aaacagacta cgtagcaaga gaacgtttag caggagccaa agaagcggtc
     1861 caaaatgatc catcgatcca attagaggtc tttacaggag actattctag aaaaagcggc
     1921 tattacgggg caaaagcatt attagaaaaa ggggcaactg ctatttttgt cagcaattac
     1981 aatatgtcga ttggtgccat cgaatgtttc aatcaagaag gtgtcagaat cgggaaggat
     2041 attgcgttca gtcactatga ttatttagat aaaaacgatc tgagtatcct tccgaaaatc
     2101 acgatcacgc caccaacaga taaaatcggg gaacgagcag cagaacagct gttaaaacga
     2161 atcaaaaaag aagaggaaga aaatgaaaaa gtgattgtga tggaaaatat catacatgga
     2221 atcaactaaa acgaactaaa aaagaaaaag aggtgctttg atgaaaaaat atcaatttcc
     2281 agcaaatttt tggtggggtt ctgcagcaag tggcccacaa acagaaggaa ggatttcagg
     2341 cgatggaaaa ggagagaaca tttttgatta ttggtacaaa aaagaaccgc aaaagttctt
     2401 tgatcaagta ggacctgaaa aaacctcgca agtttatacg aagtatcaag aggatgtaca
     2461 gttgatgaaa cagactgggc ataattcatt tcgaacatct atccaatgga gccgtttgat
     2521 acctgatgaa tctggaacaa tcaacgaaga agccgttcgt ttctatcatc gttattttga
     2581 tgagttgatt gaaaacggta tcgaaccctt tgtgaatctg tatcatttcg atatgccgat
     2641 gtatctgcaa gaaaaaggcg gatggttgaa ccgagaaacg gttgaagcct atgaaaaata
     2701 tgccaaaagt tgttttgaat tgtttggttc gaaagtaaaa aaatggttta ctcataatga
     2761 accaatcgtt ccagtcgaag caggttattt atatcgatgg cattatccag aagagtcaga
     2821 tatgaaaaaa gcgatccaag ttggctatca tgaagcgttg gcttcagcgt tagccatcaa
     2881 atcttaccat gaaatggagc aaggtggtgt tattggtatc atcttgaatc tgacaccaag
     2941 ctatccaaga gacgaatcaa accctgaaga tgttaaagca gcgcaaatag cagatgcgtt
     3001 cttcaatcgt tcattccttg atccagctgt aaaaggcgag tttccaagtg aattgattga
     3061 gatagtaaaa gaacttgatc tcatgcctgt gatccatgca gaggatttga cggttatcaa
     3121 agaaaataca gtagatcttt taggtatcaa ttattaccag ccacgccggg taaaagcaaa
     3181 agaaacacca atcgatacta aaaacggtcc aatgcctgag gattatttcg acaattatga
     3241 aatgcctggt cgtaaaatga atccttatcg cggatgggag atctatgaac aaggaatcta
     3301 tgatattttg atcaatgtac gagatcatta tggaaatatc gactgcttta tttctgaaaa
     3361 cggtatgggt gtagaaaacg aagcacgatt tatcaaagaa gatggcatga tccacgatga
     3421 ttatcggatc gaatttgtcc aatctcatct gaaatatgtc catcaagcca ttcaagaagg
     3481 tgccaattgt ttaggctatc atatgtggac atgtatggat aactggtcgt ggaccaatgc
     3541 atataagaat cgatatggct ttatttcagt agacttagca caagatggaa aaagaacaat
     3601 caaaaaatcg ggtcattggt tcaaagacgt atcagataaa aacggatttg aggcttaaga
     3661 aagaatagtt gagagcaaat gcgtgaaatt ctccattctt accattttgt gaaaaagaag
     3721 ttcaattatt tacgatcttt ttcaactttt ttgtctttct atttcatttt ttgtatataa
     3781 tagaatagtc aaaattaaat aggagtggag gaaagcatga ggaaaaaaag accgtttctt
     3841 ctcttttggt tattcggttt tttcttactg cttattttat ccgcagcatt ttcaggaaaa
     3901 atgacagcag atcttgctca gaaagaaaca gaaaaaatcg tagcaagcaa agaatcagta
     3961 gataatttca aaaaagaatt ctcaacttat tggtgggcgg agtataaaat aactgccaat
     4021 tacgaaaaag gcgaattgga catttatttg ccaaaaggaa aagaagaaaa tcaatttatt
     4081 aatatcgatg acgtacttag tgcgatttct ctcagacaat atcaatttaa agacaagcat
     4141 ttgaccgttt tgcttttgac tcatgatggc gacaaagtag cccgtttgaa aaatggaaaa
     4201 gtgatcaccg cttattctga aaaagtagac aaaaaagtgg tcgccgatcg tttagcggaa
     4261 tggaaaaaga caaatgaaca agtcaagaaa acaactgtct cttctcaaaa aaacgagtcg
     4321 acaggaacaa gtccttcaga ttctggcagt ggacaaacga taacgggaag tgaagaaaat
     4381 caagcctcaa ttattacgaa tgagtcatca caaggacaat atcaatccta tggacaatga
     4441 aaaacagagg ttgtgaaatc agcgtt
//
LOCUS       DAZKOU010000138         4461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000138 DAZKOU010000000
VERSION     DAZKOU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.145"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            574..1008
                     /gene="bacA"
                     /locus_tag="ACG827_002737"
     CDS             574..1008
                     /gene="bacA"
                     /locus_tag="ACG827_002737"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFQ0484817.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="ACG827_002738"
     CDS             2298..3221
                     /locus_tag="ACG827_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0484818.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="ACG827_002739"
     CDS             3237..3776
                     /locus_tag="ACG827_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0484819.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DAZKOU010000139         4425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000139 DAZKOU010000000
VERSION     DAZKOU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.146"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG827_002740"
     CDS             690..1658
                     /locus_tag="ACG827_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0484820.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="ACG827_002741"
     CDS             1726..2484
                     /locus_tag="ACG827_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0484821.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG827_002742"
     CDS             2552..3262
                     /locus_tag="ACG827_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0484822.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACG827_002743"
     CDS             3298..>4425
                     /locus_tag="ACG827_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0484823.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZKOU010000140         4386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000140 DAZKOU010000000
VERSION     DAZKOU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.147"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACG827_002744"
     CDS             <1..792
                     /locus_tag="ACG827_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0484824.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ACG827_002745"
     CDS             889..3138
                     /locus_tag="ACG827_002745"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0484825.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..4316
                     /locus_tag="ACG827_002746"
     CDS             3141..4316
                     /locus_tag="ACG827_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0484826.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
BASE COUNT         1529 a          628 c          805 g         1424 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctagagtaac ccgtggtgct agttaatttt
     4321 aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga cttaatagaa
     4381 cactgt
//
LOCUS       DAZKOU010000141         4377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000141 DAZKOU010000000
VERSION     DAZKOU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.148"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG827_002747"
     CDS             73..234
                     /locus_tag="ACG827_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0484827.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACG827_002748"
     CDS             253..1530
                     /locus_tag="ACG827_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0484828.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACG827_002749"
     CDS             1540..2196
                     /locus_tag="ACG827_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0484829.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACG827_002750"
     CDS             2196..3572
                     /locus_tag="ACG827_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0484830.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..4115
                     /locus_tag="ACG827_002751"
     CDS             3702..4115
                     /locus_tag="ACG827_002751"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0484831.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4140..4377)
                     /locus_tag="ACG827_002752"
                     /pseudo
     CDS             complement(4140..4377)
                     /locus_tag="ACG827_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1699 a          522 c          761 g         1395 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DAZKOU010000142         4262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000142 DAZKOU010000000
VERSION     DAZKOU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.149"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..499
                     /locus_tag="ACG827_002753"
     CDS             62..499
                     /locus_tag="ACG827_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484832.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            594..1478
                     /locus_tag="ACG827_002754"
     CDS             594..1478
                     /locus_tag="ACG827_002754"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0484833.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1700..>4262
                     /locus_tag="ACG827_002755"
     CDS             1700..>4262
                     /locus_tag="ACG827_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0484834.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1394 a          714 c          960 g         1194 t
ORIGIN      
        1 tttttctgta taatcttaaa taaagaaaac gatattaaaa aatgcacgta gaagtagata
       61 aatggagtgg ttttgcaaat ttcttttatt tctagaagtt attcacgtat acggatttgt
      121 ttttctcaca aataaaaagg caggaagtac aatggatcat ttgtatataa aatatattca
      181 tcaattttcc gttttggtat taggcacact aattatagcg gggattgttg catttattat
      241 tcaaggtcgt gacatattaa cagtacatca agagtttttc agttatgcat ttggtattcc
      301 tctgatcgct ataaggtctg ataacaaaaa gtggaagaaa cgctattaca ttctcttgct
      361 atttttgtgt ttttatctgc cacctaagat ttttacttta ttaggtatac aatgcctatc
      421 attttctttt atttcctata tcattgtttt aattttcctc agtttcatag tatgggtatt
      481 ttatcttgtc aaaaaataaa tgaatgtttc atgaacagta cttcatcaca gcctaatttt
      541 tttctttatg ctatcgttat attaaagaaa tcaatcagaa gggaaagaaa gtgatgaaaa
      601 aagttttatt agtggtaaac cccagttcag gcggtgaaca agcaaaggaa tttgagcaat
      661 tagcgatagc aaaattggaa tcggtgtttg atgaagtggt cgtcttgcat acaaaaaaag
      721 caggggatgc aaaaaacttt actcgcgaag cggctacgga agggtatcat agtgtgtttg
      781 tcatgggcgg agatggaaca gtcaacgaag gaatcagtgg aatcgctgaa caagaacatc
      841 ggccaaattt cggatttttc ccattgggta cggtgaatga cttggctcga gcattaggga
      901 taccgctaga acctgaggaa gcgatcaatc atttttcgat tgaatcagtc aagccattgg
      961 atattggaaa aataaatgac gactatttta tgaatgtcgt cgcaattggc tctatccctg
     1021 aagcaatcaa tgatgtagat gcggagaaga agacgaaatt tggaaaacta gcttatttca
     1081 tgtcaggaat caaacagtta gcttccacac aatcctactc gtttcacgta gaagttgatg
     1141 gaaagaagga agaaattgag agcagtatct tgctgatagg actaactaac tcagtaggcg
     1201 gttttgaaac attacttcca aatgcaaaag tggatgatgg taagctccat ttggtttatt
     1261 taaaagattc ttctttgatc gatacgctta aaacattgcc agacctatta aaaggagtag
     1321 atgaatctac gaaaaatcta gcctatctta cttgtgagca attagttgtg tcattagctg
     1381 atgaggaaga attgacgacg aatgttgatg gagatgaagg ggataaatta cctgtgacaa
     1441 tcaaagtgtt accctcacat ttacaggttt attgttaaaa tggaggttgt gaccgaagag
     1501 ttgtcaggag gacatcggtt gttcggaata agggactgtg acaagacttt tgtcacagcc
     1561 tttttttgta caaaaaagaa attttcacct aacagaaaaa tttgccataa aaatacatat
     1621 tttaaatatg aaagtacaat tacaaataaa ttaaaacgtt aaaataaaat tgtcaaaacg
     1681 tcagagagga tggtggagaa tgaatcaata tgtaaaaaag ctgattgcgt cgattttgtt
     1741 tgtcttccct ctcttgatgg tgacatccgt ttttgccgat gtagagaaaa caagcgaatc
     1801 aacgcaatca gagaatcaaa gttttgacaa gactactttt tcagaagaat caattacttc
     1861 tgaaactaca gaatcccaaa gtgaggcgcc tacttctaat acaaggcaag aagaaaataa
     1921 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag aggtacaggt
     1981 tagagttgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tgacgcagct
     2041 tctcgcagac gaatcagtag ctaagattcg tttaactcag cctttgactt tagacaggga
     2101 attggagatc aagcgagata tcgttatcga ttttggcgga cttgctcata atttcggcac
     2161 acatcacatc tatatcaatg agacacctca gtcgatcgag tttcagaatt ttaaaggaac
     2221 ggcagctcat ccaggtgggt tgataccaaa tatcgatgga aatgccatta tcattgctta
     2281 tatgtccgat ttttggggaa acagatatca ttttacggga gaaataaaat ttaccgggac
     2341 acttgactta tatgatggtt ctaaattagg cctgatttac gcccctcgtg caactgtcac
     2401 attagatggt gtttccggtg tattggatgt ccaaccagtg aaagaaggga tgaatgccgc
     2461 tccgggtaaa gcttattttt gtagaagcta tcagctaaat gtaatcaatg gtagtcagct
     2521 ttatggacca tatctaggaa aattctatgg ttttctagat tcaggtgatg agacgggcag
     2581 tggaaaaaca gcaccaggac tccatatctt gagtggttca aaggtctctc ttgactatga
     2641 tcgggcagat ggcgtcagcg atgaaggaga ggctgtcgat acgctacccg ggaatgtcac
     2701 atttaaagtc agtggctcag gaagtgaatt ttcggtaaat acaaagatca atattaccaa
     2761 cgacaacaat cgcggaatta tccaaatgag aggctcagga tcaagagtcg ctgtgtcgaa
     2821 tgatgggaaa atcacaatca atacagcgac tactggcgga tttcgcctgc aaggagattc
     2881 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatggcga
     2941 ccaacctgga aacaatggga tgcgttttgt agggaaagga ctttctttga tcgtagaagg
     3001 agccggatca gtcatcgata ttgataagca aagtggtcgt tctagcgcag ttcgttttga
     3061 gaacggtacg caaaaactaa cagtaactaa tggaggctca ttgaaggtaa gaaatgcagg
     3121 ggatggcaaa tcttatgtga acagactcaa tcaaggaaac caagcgatcc aattttatga
     3181 cgcttctggt tttctagaaa gtccagggtc agctgatttt acggttactg gagaaaaatc
     3241 aaatatcgat atacgcgcag attccggagc taccgtagat acaactggag atattgattt
     3301 gaactttatg gctggcgaaa aaacttattt agtcatggtc gggaaaactg gggaagataa
     3361 gactggcatc atcagtgggg atgtgttgaa tgtagactta gaatctccta cctatttcga
     3421 tttccaaaac aaacggatcg gaagcgagac taatggcata ggtggctggg tatttcaagg
     3481 aaatggcgat tcttctttga agatcgctca ttcagaaatt gctttatggc gaaaggcagg
     3541 agtaacgttc gataactttg aaaaagatcc ggattatttt tctgatatga cagacttatc
     3601 gatcaatgga accgacttag gtaattttgt atcaagccct agtgcaggtt taacaaatga
     3661 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataaccagct
     3721 tccgatgatt gaatccttaa gagtaccaac gaatgcagat aaaaaaatct atgggcatgt
     3781 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
     3841 gactttacgt attacttatg cagatacgac gaaatcacct gtagaaatta ctgctctcac
     3901 acaaggacaa acagatgaaa atgaagggac aggatataat ccgtggggag aaggcgaaca
     3961 aggcggtttg tttgaggttc aagtacccaa cgaagaaaga ctaacaacgg gggatcaagt
     4021 gcaaatagtt gctgctaaaa aagtaagagg cggccaagaa gtgacagaac tggactctgc
     4081 caaaacgaca gtcgatgtga ttcctcctga gcctgctaaa ttaagtacca cattgatcaa
     4141 tgcagcttct cgctcaatca caggtacagg cgaagcagga gctttggttt cattgaaaaa
     4201 aaatcaagct gttttgtccg aaacaactat tgatgataca ggtaaatttg ttctttctat
     4261 cc
//
LOCUS       DAZKOU010000143         4053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000143 DAZKOU010000000
VERSION     DAZKOU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.150"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..1668)
                     /locus_tag="ACG827_002756"
     CDS             complement(163..1668)
                     /locus_tag="ACG827_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0484835.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1665..2351)
                     /locus_tag="ACG827_002757"
     CDS             complement(1665..2351)
                     /locus_tag="ACG827_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0484836.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2544..3965)
                     /gene="gndA"
                     /locus_tag="ACG827_002758"
     CDS             complement(2544..3965)
                     /gene="gndA"
                     /locus_tag="ACG827_002758"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0484837.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1116 a          857 c          692 g         1388 t
ORIGIN      
        1 ttttttcgtt catctaaaag atacttacat ttaattaacg gatgttattc tatttaattg
       61 ttcgctagtt tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt
      121 attctttgtt ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt
      181 tgcgatagga ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat
      241 acgtccttta tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc
      301 cttcgttcta gctcttgcat tatccactcg ataaaaacga ttgaagatct tattaaggtc
      361 ttctttgggt attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga
      421 agaaatagag atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat
      481 gataatgatt aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa
      541 atcatcatct aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg
      601 ataggtgact tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc
      661 tcttgataag tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct
      721 ggcgcttaat gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact
      781 aagatgccct tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa
      841 ttgctcttgt tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc
      901 taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc
      961 tttacgtata gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga
     1021 taaaaagaaa cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt
     1081 caataatagc ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata
     1141 gccgatcttt tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt
     1201 tccttctagt gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt
     1261 ttcatgggtt ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg
     1321 ctgccctagc tcagctataa aggaatcgat cttcattaag tttccttcga ccagtgaagt
     1381 atgatctacg acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac
     1441 taatgtcagt tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc
     1501 tacattgttt ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc
     1561 gaaaattgta aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa
     1621 ggagggccct ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca
     1681 taacctgttc cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg
     1741 cgaagataac gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac
     1801 actttattta gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc
     1861 agcaactcgt attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg
     1921 ttttcttttt cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta
     1981 tcgccttcaa tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata
     2041 gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc
     2101 acagaatctc ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg
     2161 cagacttcta aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca
     2221 ttatttaatg ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct
     2281 tcatgcttca actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga
     2341 atattgctca tttattattt tatcctttct attctactgg ctttttaaaa actctatcat
     2401 tataccgata atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa
     2461 agagctgaag tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc
     2521 atttcctaac agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct
     2581 tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata
     2641 tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc
     2701 ggtacaggta cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat
     2761 tttttcgtga tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg
     2821 tcataggcat ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag
     2881 atttttgcga tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat
     2941 tgagcaaatc cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt
     3001 tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg
     3061 cttgcttgta cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg
     3121 atcaatggaa gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct
     3181 ttattcccag ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct
     3241 ttacgtgtca gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc
     3301 cattctttaa agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag
     3361 tcataagatt cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc
     3421 acataatgtc cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct
     3481 tttgcagaaa tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca
     3541 ggcatgattg aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata
     3601 aagttgatgc cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta
     3661 tttcctccgt caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt
     3721 gcatctgttg ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat
     3781 tctacaaatt cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact
     3841 acagcggttg ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct
     3901 atgttcaagg ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt
     3961 gacatatgaa ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac
     4021 acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAZKOU010000144         4028 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000144 DAZKOU010000000
VERSION     DAZKOU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.151"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..519
                     /locus_tag="ACG827_002759"
     CDS             <1..519
                     /locus_tag="ACG827_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484838.1"
                     /translation="FLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKAEKFCL
                     LLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKP
                     PKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPN
                     KNNNFYFYRNKL"
     gene            complement(645..1322)
                     /gene="nth"
                     /locus_tag="ACG827_002760"
     CDS             complement(645..1322)
                     /gene="nth"
                     /locus_tag="ACG827_002760"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0484839.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1329..2027)
                     /locus_tag="ACG827_002761"
     CDS             complement(1329..2027)
                     /locus_tag="ACG827_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0484840.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2295..3479
                     /locus_tag="ACG827_002762"
     CDS             2295..3479
                     /locus_tag="ACG827_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0484841.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3485..3928)
                     /locus_tag="ACG827_002763"
     CDS             complement(3485..3928)
                     /locus_tag="ACG827_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0484842.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1059 a          785 c          655 g         1529 t
ORIGIN      
        1 tttttgaaag aaggcttcat tttgagttat ctgatagtat gcttaattat cataatttgt
       61 ctgcaccatc tttttttatc gaaaacaata cctcgttatt ccaagaataa aaaagctgaa
      121 aaattttgtt tattattaaa taaatttact ttaattgctc ctatccttgc atttattatt
      181 ttttctgtac ttttaagcac tactcttaaa ggaaaattta tggaaagaag ctctcatgca
      241 atgattctca cttttttatg gttgttgttc accagaatct atatttttct gatgagcctg
      301 aaaccgccca aatccattag tttatgttta gttataaatg gtattttttt attatcactt
      361 atcattttta ttacaccttt agatagatac gtcacttatt tatataatcc tttggaatac
      421 tggacttatt ttataggtat attggaaggc attattttct acatcgggta ttttcccaat
      481 aaaaataaca acttttattt ttatcgcaat aaattgtaat aagttaccat cctagctact
      541 gagtatgtac atttactttt tcatgcaaaa aagataaacg aaacagcttt tctccataat
      601 aacaagataa ttcatacatt tatttgcaat taaagaaacc acgtctacag gcgtggtttc
      661 ttttttagcg ccttttcttt tagacgcatc cgatttttcc catcttgaca tattgatagc
      721 aaaggacata cctcgcactt gggatttctt gccgtgcaat gatatctacc aaaaaaaatc
      781 agtgtatgat gtgtctttac ccataattct tgtggtactt tccgcatcag tgtttcttct
      841 acttccatga ccgtagcatc tagtttgcag atcctcaggc gttttgagac acgttccacg
      901 tgggtatcta ctgcgattgc tggtatcccg aatgcatccc ccaatacgac atttgctgtc
      961 tttctgccta caccaggaag agacatcaat tcttctcttg acgttggcac ttgcccatcg
     1021 aaacgttcaa tcagctgctg tgcacacgct ttgatgtttt ttgctttatt tcgatacaaa
     1081 ccaatcgttt ttattttaag aatgatttca tcgattgaag cttctgctaa cgcatcgggt
     1141 gtcggaaaag aggcaaacaa atctggcgtt gctttattca ctgatacatc tgtcgcctgg
     1201 gcacttaaga tcactgcaat caataactca aatggattat tgtgcttgag ttctccatgt
     1261 gcttcgggaa acattccata catcgtttct agagcttcca ttgttttttg tttgcttaac
     1321 atattccgct actcctccaa ccaatttttc agtgagactt tcggcaattc ttcttgttga
     1381 gctgactgtt ctacttctct ttgaagaagc tgctgttttc ttcgtttttg gtcttcttcc
     1441 acttgttgtt ttgtacgtag attttttctt tcccacgata gcaaaatgcg gtcaacgtaa
     1501 ttaaaactat aagcctgatt taatacggct tcccgcagtg ctagcttcaa aatttccggc
     1561 tgataatggt cttcttctag ccattgtccg atccgctgga actcgatcga agaaagcggg
     1621 cgtccaaatt cttgttcaaa taattgatac actgactgga tttctttttc ttgtgttttt
     1681 tcttcgtatt ttttttcttg tgtttttaaa tactcaccta atagatcata gatggggtaa
     1741 agattatatc gatctgcttt tttaccgttg acaatcgtcg tttctatctt gataaatcca
     1801 tttgttacga gacgatccag tatttgaaaa atctgatctt gtttgattcc catatccata
     1861 gcaatcatct gcaaatcagg gaaagtatct ccttcaagct gtgccatatg caactgtaga
     1921 acaaataaaa attcttcaga ttgtagaccg atcctatgat aatttttaag taaaagatta
     1981 gatatgactg tttgtccagc gcttaagtaa ttttctaaat tcaacatgat ttctcctccg
     2041 ctcaatcaca gtatacgtaa aaaaaacatg ggaagcaaga acaggtttct tttctattta
     2101 cttcactgat cggtcagatt tacaatgtcg tctacttgat ttttgtttgt tcatgttttt
     2161 gatgcgtatc ttcacttttc ggaataaata aattaagtag atttcgctgt tccttagaat
     2221 gttgacaagg aaacaaaatt actatacact atttttgttt ccaagttaac attattgcat
     2281 aaaggagttt ttacatgtac agtttcacaa ataaacattc taaagttttt ggatacgtct
     2341 ctgtcttatt cactggatta ggatttacta tcgtcagtcc tgtacttccg tttttaacgc
     2401 tgccttattc tcactccata catcagcaag ctttctatat cactttgctc atgtctgttt
     2461 atgctctagc tgccttctta tctgccccca ttctcggtag cttaagtgat cattttgggc
     2521 gtcatccgat ccttattatc agtttattag gatcaagtat cggatatttg ctttttggtt
     2581 taggaaattc gatatggatg ttgttccttg gtcgtatcat tgaaggattg acggcaggag
     2641 aaatctccac cttatatgct tactttgccg acatcacgga acctaatgaa agaaccaaag
     2701 tttttggatg gatgggtgct ttagtcggta taggaacaac gctgggtcct attatagggg
     2761 gtctattagc tgagttgggg aattctgttc ctatatttat tggagcactc tttactttct
     2821 taaatgccgt ttatggctac acatttatgc tagaaagcct tccaataaaa aaacgttcag
     2881 tagatttaag tttctcccat gtaagacctt ttcaccaatt acaacaatta ttcaagattt
     2941 catccgttat tcctttattg acagcaggtt ttgctgtttg gttggcagct ggctctcttc
     3001 aatctatctt ttctcaattt tcgattgacg cctttcagtg gaaggctggg ttggttggtt
     3061 taagcttctc tttgattgga atcttggata ctgtctcgca attgttcatt atgcctcgat
     3121 tgctcaaaaa attttcagaa cagcagatca caagaatagg gatgttcagt gaaatcctcg
     3181 cctatctatt catcacatta tctggaattc ttctactgcc gattttgttt ttatttggga
     3241 ttatctgtta tggtttcggt gattctattt ttactcctgt attcaatggc caactatcca
     3301 attctgtctc agataaccag caaggtcttg taatgggcgg tacccaaagt atacaatctc
     3361 tatcgcgcgt catcggcccg ctaatcgcag gacagcttta tgctgcagca ccatttcttc
     3421 cgacggtatt cggatttttg cttatgattt ttgcctatta tcactacaat aaaaaatagt
     3481 agcttcattt gctacttcgt ataaattctc tgtatcgacc gataaaacga aatactgtgt
     3541 caaattcttc ttttgacata ttttcaaata cagtagcatc tcgctgtacc catttttgat
     3601 ggagttgctc gtgtctattc cttatttttt gtccgattgg cgtcaattga aaatagattt
     3661 cttttttatt ctcttcgctt tgataagtgt ttatgatccc tctagctatc agtttttttg
     3721 ttagcttact gatggctcct ctagtcatgt aactagcatt cgctaatttt gtcacgttag
     3781 gatcagggat tttcccgata tgttcaatca cttctagttc atttagagta aatcctgcaa
     3841 attctttttc catttctggt ttattacgat gaataatctc attagctaaa tccagatatt
     3901 cttctattag cagttgtctt ttatccattt ctacaccttc tttttctttc attatattgt
     3961 ttttttgttt cctattcaac aaagataaaa atattttgta ggaaaaccca atctgaaacc
     4021 aagaaaaa
//
LOCUS       DAZKOU010000145         4011 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000145 DAZKOU010000000
VERSION     DAZKOU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.152"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            178..774
                     /locus_tag="ACG827_002764"
     CDS             178..774
                     /locus_tag="ACG827_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0484843.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="ACG827_002765"
     CDS             898..2697
                     /locus_tag="ACG827_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0484844.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="ACG827_002766"
     CDS             complement(2975..3187)
                     /locus_tag="ACG827_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484845.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3649..3798
                     /locus_tag="ACG827_002767"
     CDS             3649..3798
                     /locus_tag="ACG827_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484846.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1179 a          686 c          709 g         1437 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg agatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagcagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat t
//
LOCUS       DAZKOU010000146         3925 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000146 DAZKOU010000000
VERSION     DAZKOU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.153"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACG827_002768"
     CDS             complement(573..1130)
                     /locus_tag="ACG827_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484847.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACG827_002769"
     CDS             complement(1309..1920)
                     /locus_tag="ACG827_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484848.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACG827_002770"
     CDS             complement(2033..2290)
                     /locus_tag="ACG827_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484849.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACG827_002771"
     CDS             complement(2887..3165)
                     /locus_tag="ACG827_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484850.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACG827_002772"
     CDS             complement(3199..3450)
                     /locus_tag="ACG827_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484851.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DAZKOU010000147         3863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000147 DAZKOU010000000
VERSION     DAZKOU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.154"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..466)
                     /locus_tag="ACG827_002773"
     CDS             complement(245..466)
                     /locus_tag="ACG827_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484852.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACG827_002774"
     CDS             complement(564..809)
                     /locus_tag="ACG827_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484853.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACG827_002775"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACG827_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="ACG827_002776"
     CDS             complement(2033..3034)
                     /locus_tag="ACG827_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFQ0484854.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="ACG827_002777"
     CDS             complement(3193..3459)
                     /locus_tag="ACG827_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0484855.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="ACG827_002778"
     CDS             complement(3449..3805)
                     /locus_tag="ACG827_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0484856.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DAZKOU010000148         3780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000148 DAZKOU010000000
VERSION     DAZKOU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.155"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG827_002779"
     CDS             complement(<1..2232)
                     /locus_tag="ACG827_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0484857.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG827_002780"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG827_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1101 a          806 c          580 g         1293 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaacaatca ttcttaaaaa gaatatataa aaataacaat aacctttgtg atttttcttg
     3601 tttgaagaat gttagaagaa aaatctctaa atacttataa ttattttata agcaaaaaaa
     3661 tgctatcggt agtatcaccg atagctgagg aaagtcacag tagggactat ttgattaaaa
     3721 aatatgttta ttcaatccat ctatttattt ttgccttatt aacatattgt aataatgtaa
//
LOCUS       DAZKOU010000149         3665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000149 DAZKOU010000000
VERSION     DAZKOU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.156"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..278
                     /locus_tag="ACG827_002781"
     CDS             <1..278
                     /locus_tag="ACG827_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFQ0484858.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            421..642
                     /locus_tag="ACG827_002782"
     CDS             421..642
                     /locus_tag="ACG827_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484859.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            658..1500
                     /locus_tag="ACG827_002783"
     CDS             658..1500
                     /locus_tag="ACG827_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484860.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1563..3365
                     /locus_tag="ACG827_002784"
     CDS             1563..3365
                     /locus_tag="ACG827_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFQ0484861.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3424..>3665)
                     /locus_tag="ACG827_002785"
     CDS             complement(3424..>3665)
                     /locus_tag="ACG827_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0484862.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1308 a          519 c          746 g         1092 t
ORIGIN      
        1 aagaactcat caagctggct aataagggtc aagattatga acatctagca gatgagattg
       61 atgagctgag agacaagcga cagctccttt tagtagaaga tgcctccctc agtggcgaga
      121 acgagcgaat caatgagctg attgaattta tccgcaagaa caaattccgt accttagagt
      181 acgatgataa gcttgtaagg aagataatcc agagcgttac agtctatgaa gaacacttcg
      241 tcatatcctt caaatctggc atcgaaatgg aaatatgaaa accagaagta aatagcccat
      301 gattctacag tagagtcgtg ggttttcttt tgcttgctga ttataataat attttaataa
      361 ctattgttat tataaaccaa atgatttata atattcttag cgtaaacttg gaggtgctgt
      421 atgactacgg cagaaatgat taaagaactg tgtgagcaaa tgaatataag tgtttccgaa
      481 cttgctagac gtattggaca gactccacag aatttcaata agaaattaca acgagaaacg
      541 gtaaccttag atgagttgaa agcgattgct gatgtgctag gtgtcaagtt tgtgcaggct
      601 tttatattgc ctgatggcaa tgaaataaag ataggtaatt aggaggtgta atttgtattg
      661 aaaatcataa caataaatga tgttgagtat gctgtatttg ctgctaatga aggtacttcc
      721 aaaccacaac ctcatatcat agaaacgaaa tctggtacta ttccagaagg aaaacaactg
      781 agtttattaa aagaatatct taagcagaat gatatttccc caataaaagg agcaacaaca
      841 tattggtgca ttgacaaggt tcttaagctt gattcaagta aagaaaaaac tataagtgaa
      901 acaatccata aacaaaaata cttatcttta actgaagaaa atattgagaa acagcataag
      961 tttgtaggtg caagctctaa ttatgggaaa gaaggtctaa taatacatga tgttttgaat
     1021 gcatttcctc ttcataatga tttaaatact attgcgatga agattgcggt aattgatgta
     1081 acaaattcaa cacatctttc tcaatataag agcaggttgt ctctatatga tttggctaaa
     1141 gtaattctgg agattccaaa ttttgatgat cggttggcaa aaggagatcc tcagttgata
     1201 aatattatcg caaggaacat tggtgcagtt aatatgttct catttgcatc caagtactgt
     1261 acatatcata atgtggaagt gtgtgggaga gatgattact ccatctttga tggtatcgtt
     1321 aaaaatactc ttccacacta tatccaagga ctaactacga ataagattga tacgtggcga
     1381 agaagttttg attacgaggc atttaacgaa tgtgttggaa aactacttga tgaaaataat
     1441 atacatattc cttttagaag aagaaagtta gatcacttct tgtggtatgc gaataggtag
     1501 aatataagaa aaaggcatcg taaaagtgat tctggataaa attaatgggg gaggaaataa
     1561 tagtgggatt atttgatttt ctaaagaaaa aacaaggaaa ggttagtaaa cacgttgatt
     1621 atcaactggc ttctagtcta tttcgtactg gtaaagtttt tgctgaaaaa ttggaagtgg
     1681 acatatcaaa tgtagatgag tttcgtaaaa cttttatcgc ttttgatgtg gaaacaactg
     1741 gcctaaattc ttcttcagat agaattgtag agattggtgc ggtgctattt aaagaaggag
     1801 ttccaattca atcattcgga actcttgtca atccaaaagt tagaattgca gcctcagctt
     1861 cggcaataaa taatataact aatgaaatgc taaactctgc tccaaatgag caggaagttt
     1921 acccccagtt ggtagaattt ttaggagatg cactagatgg aaaaatcact atgtgtgcac
     1981 acaatgcaag tttcgatttt ggatttttaa gtaacacatt ttctagactt gggtatgatg
     2041 ctaaaattaa gtatgttgat actctgagtc tctctagaaa acatataaaa ggcattgtaa
     2101 atcataagca agagactgta gcggaatatt tcggattaat aaatgacact gcccatagag
     2161 cggaatcaga tgcagatata tgcggaaaga ttttttgggg tgttttagat attattgagg
     2221 agtcgttgga agaacaaaaa aagcaaatag aaaagatgac tccagacata aaagaacttg
     2281 aagcttgtgc ttttattcaa gatattattg agaaaaaagg atgcgacacc agttggatca
     2341 ggtatcgaaa aaacagtaat aattatgtcg atgtaacttg tctatataca ttcttaaagt
     2401 ttaagtttgc taagaaggga aattacatca ttattagaaa agatgctaca aatggaattg
     2461 atttacctat agaaccttgt actgtaagtg aaggcggtac aacaaatgtg cgagtctatt
     2521 ttcgtagccc ctttgactta acgcctcttt caaattatat tttcagtgct tatagcgatt
     2581 gttataattc tatgaatgaa tatatgagtg ttagtaatta cactaaaaaa gaagcagagc
     2641 aaagccttag tatgctgaaa tctatttcaa aatcagcgat gaaagagtta ttattcgatg
     2701 caaaaaatcg tggatatgat gagactgaag ttaacctaaa tattgaaaaa gtgatatcta
     2761 gagaagatgt aattattaac gggttgcata atcgagtttc attaaacaag atacgaaata
     2821 tagggaattg ggagaaagga tttgatgcag gttatccata ttgggaacgt ggagaaactg
     2881 ctaggaaaaa tggcaaagta gatgaagcta ttgcactttt tgataaagct cgatataacg
     2941 gctatgatgc tcctgcgcta tatgattctt atgctaaagc ttatcgaaaa attaaggatt
     3001 acgataatga aattctcatt cttgatgaag gaattatgcg taaaacacgg cacgatgttg
     3061 gtactttaga ggctcgtagg gataaggcaa ttaagctatt atttgctaaa caagaggcag
     3121 agcggatagc taaggaaaaa tctgattttc taaaagcgaa caaaaaagag gatgcgatta
     3181 gtgagcctag caagaagcgt ggaagacaga ttattcaaat gactgatgat ggaacaataa
     3241 taaaagaatt cgatactata gcttctgctg taaaagaagt tggcgttaac tcaaaaagta
     3301 ttagggatgc tgcaaatgga gttcaaaaac atgctggagg atactactgg aaatacaaag
     3361 attagcagat aatctaagat aacctaggat cttcttaata gatacccgtg gtgctagtta
     3421 attttaatat cctaaagaat atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa
     3481 tagaacactg taagccagta atatgctttc aaatctactc tctagccctt taacagaacg
     3541 tgagttgaaa ttttgacaac ctaatttttc taaggaagaa aaaacagtcc caactgtttt
     3601 tctttgtttt ccaagtaagc tgtggtctag tttttctgat ttatccatgt ttttacgtgg
     3661 cggaa
//
LOCUS       DAZKOU010000150         3624 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000150 DAZKOU010000000
VERSION     DAZKOU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.157"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..563
                     /locus_tag="ACG827_002786"
     CDS             <1..563
                     /locus_tag="ACG827_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0484863.1"
                     /translation="KFEKKKYVLQVFHPVVTELENIEKQILALINSMEQQSQPIICIL
                     PNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFK
                     IPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSS
                     RRITKTLNQILNDGEDIKWIQKQIAY"
     gene            536..1213
                     /locus_tag="ACG827_002787"
     CDS             536..1213
                     /locus_tag="ACG827_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0484864.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1164..2345
                     /locus_tag="ACG827_002788"
     CDS             1164..2345
                     /locus_tag="ACG827_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0484865.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2358..3335
                     /locus_tag="ACG827_002789"
     CDS             2358..3335
                     /locus_tag="ACG827_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0484866.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3335..>3624
                     /locus_tag="ACG827_002790"
     CDS             3335..>3624
                     /locus_tag="ACG827_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0484867.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSES"
BASE COUNT         1367 a          451 c          680 g         1126 t
ORIGIN      
        1 taaaatttga aaagaaaaaa tacgttttgc aagtatttca tccagttgta accgaactag
       61 aaaatataga aaaacaaatt ttagctttga tcaattcaat ggaacaacaa tctcaaccaa
      121 ttatttgcat tttacctaat agtgatgctg gtagcgaaat tatcagaaaa atgtaccaga
      181 aatatttaaa aacggatatt atttatatta cgaatttaga gccggaggaa tacctgacta
      241 tattaaaaga gtgcaaattt atgatcggaa attcttcttc tgggataatt gaagctgcat
      301 cattcaaaat accagtaata aatttaggaa ccagacaaaa tggacgagaa caatctaaaa
      361 atattataaa tgcaaatcta aatgaaaatg aaattgaaac tgctttgaat aaaatacaag
      421 atcctcagtt tataaaaagc ttgaaaaatg ttaagaacat ctacggtgac ggcaacagtt
      481 ctagaagaat aacaaaaaca ttgaatcaaa tattgaatga tggggaagat attaagtgga
      541 ttcaaaaaca aatagcttat taaaatatac tagtgatcca tacattatta ttggggctgg
      601 cggacacgca aaagtcatta ttgaaacctt aagattagaa aataagttaa tatatggatt
      661 ttttgatgat aagcaaaata aaatgcaaga tctgaaatat cttgggaaaa taaacgaatt
      721 gaatagctgg gcggatagca aaaaaaaaat ctataaattt attgtagcta ttggtgactg
      781 tgtagtaaga aaaaagatta taaaaaaatt gaatatgcca agtgagcaat atggaatagc
      841 tgttcatcca agtgctatag tggcaaaaga aatttcaatt ggttatggaa ctgttatttt
      901 tgcgaatgct gtaattaacc cagatgctgt aattggagag catgctataa taaacacagg
      961 tagcattgtt gagcatgatt gtagaattaa taactatgtt catttatctc caggtgtatg
     1021 cttagcgggt ggagtacatg taggtgtagg aactcaaata ggaattggtt ctcaatgtat
     1081 ccagatgaag aaaattggat cttggtgtat gataggcgca gggagcacta ttgttaagga
     1141 tattccctca cattccttag cttatggcaa tccagctaaa attaagaaag aaggaattaa
     1201 ttttgaattc tagaatttta ttatcaagtc ctcatatggg aggaacagaa gaaaaatatg
     1261 ttgctaaggc ttttaaagat aattggatag cgccattagg tgaaaatgtg gataaatttg
     1321 aagaaagtat tacaaaatat actggttcta agggcggagc ttctgcgtta agttcaggta
     1381 cagctgccat acatcttgcc ttaagactgc ttaatgttgg aaaaaacgat attgtctttg
     1441 ctccttcatt tacttttata gctacagtaa atcctatttt atatcaaggg gctgtaccag
     1501 tgttcattga ctcagaacct aatacatgga atatgagccc tgttgcacta gagagcgcat
     1561 tagaagaagc tgaaagaaac aaccaattac ctaaggcaat catcgtagtg aatttatatg
     1621 gtcaaatggc tgattatgaa cgaattttag ctattgcaaa ccaatttaat gttccagtga
     1681 tagaagatgc agcggaatcg ttaggttcta cttataaggg aatttcgtca ggcttatttg
     1741 ggacaattgg gatctattca tttaacggta ataagatcat cacaacctca ggtggaggag
     1801 ctctagtgtc tagtgataaa aaatatgttg agcattcaag atttttagca acacaagcaa
     1861 gagatcaagc accgtattat caacatagcg aaataggtta taattaccgt ctaagcaata
     1921 tttctgccgg tatcggacgt ggacaaatgg aagtattgaa tgatagagta gctaaacgaa
     1981 gagaaatttt caatcgatat aaggaagctt tttctggaat tgatagaatt gaaatgatgc
     2041 cagaagtgga tggcatggtg ccaaatcgtt ggttgtctac tttaacgatc aatgattata
     2101 attctattga tcaagtagtt aacgtaataa ataaactaaa tgaaaaaaac attgaaacaa
     2161 gagctttgtg gaaaccacta catttgcaac cagtatttca aggggtaaaa atgtattacc
     2221 atgataataa tcttagtgtg tcagaaaagt tatttaatag cggcttatgc ttaccttctg
     2281 gaagtaatat gacagatagt gaacaagaat ttgttattga tgagtttaaa aaaatattca
     2341 agtaggtgtg atattttatg aacataggaa ttattggttt aggttccatg gggaaaagaa
     2401 gacttagact cattcaagaa cactttaatg atattttact atgtggtgtt gatactaatg
     2461 aggcgagacg aaaggaagtt gaaaaggaat ttggaatacc aacttataat agtctaacag
     2521 aggcaaaaaa tttgtttgaa ataaattcag tttttgtttg tacatcccct ataacccatg
     2581 aatttattat aaaagaggct ttaaatttag attgtaatat ctttacggaa atcaatttat
     2641 taaatgaata ttatgatgaa ataatcgaag tagctaaaaa gaaagataga ttactttatc
     2701 tatcttctac atttttgaag agaaaagaaa ttcaatatat taagtctaaa gtgacgcata
     2761 ataaaaaaat ttcttatcaa taccatgtag gacagtattt acccgactgg catccttggg
     2821 aagattatcg caattttttt gtttcaaaca aaaaaactaa tgggtgtaga gaaatattcg
     2881 ctattgaatt accttggatt atagatacat ttgggaaagt taaatcgttt actgtaatga
     2941 cccagaaatt atccaatctt gatattgatt attcggacag tttcactctt attattgagc
     3001 atgaaactgg tatagttgga actttaagtg tagatgttgt ttcaagagta gctaaaagaa
     3061 atttaactat aattggtgaa aagattttaa tagaatggga aggtaacccg gattctctat
     3121 tttcatggaa tgaagaaact tctagtatga cagatattca attgtacgat gaggttatgc
     3181 atgataataa ttattcacat actattattg aagatgcata tttagaggag attaaagaat
     3241 ttttcacgct tttggaaaag aaaatggaac cccaatatac ttttgaaaaa gataagtata
     3301 ttattaactt aattaacgac attgaggtaa attaatgatg aaagtaattt tttttgggtt
     3361 agggtcaatt ggaaagagac atctaaaaaa tctaataagc tatggaaaaa agaattctat
     3421 taattttgat ataacagctt atagtagcag tggaaaaaaa gagtttgaag aaataacgta
     3481 tattcaaaat agagacgatt tggatacaca ttatgatata gcttttatta caaatccgac
     3541 ttttaagcat ctggaaacac taaatgtaat tgttgaaaaa gttgattaca tatttttaga
     3601 aaaacctgct ttttctgaga gtat
//
LOCUS       DAZKOU010000151         3602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000151 DAZKOU010000000
VERSION     DAZKOU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.158"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG827_002791"
     CDS             <1..186
                     /locus_tag="ACG827_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0484868.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACG827_002792"
     CDS             329..553
                     /locus_tag="ACG827_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484869.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACG827_002793"
     CDS             568..1437
                     /locus_tag="ACG827_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0484870.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACG827_002794"
     CDS             1418..2152
                     /locus_tag="ACG827_002794"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0484871.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACG827_002795"
     CDS             2185..3093
                     /locus_tag="ACG827_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0484872.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACG827_002796"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACG827_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DAZKOU010000152         3540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000152 DAZKOU010000000
VERSION     DAZKOU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.159"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(266..970)
                     /locus_tag="ACG827_002797"
     CDS             complement(266..970)
                     /locus_tag="ACG827_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484873.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1157..1402)
                     /locus_tag="ACG827_002798"
     CDS             complement(1157..1402)
                     /locus_tag="ACG827_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484874.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1419..2270)
                     /locus_tag="ACG827_002799"
     CDS             complement(1419..2270)
                     /locus_tag="ACG827_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0484875.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2436..3440
                     /locus_tag="ACG827_002800"
     CDS             2436..3440
                     /locus_tag="ACG827_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0484876.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1098 a          603 c          687 g         1152 t
ORIGIN      
        1 ttttttctct atcagaaaat atgtttatta ttagaatttt ttatagttaa ggttaggcat
       61 cttaagaaat caggcaactc caagatatct tcaggtagaa gagaagtcgc ctaacattgt
      121 aggatcatag gtgatttgtt ggacaggtca agctgatatt tgagcctgat gttagaagga
      181 atttcttttt acttgctgta gaaattatta tatgactaaa aaggctggga ttttaaatcc
      241 cagcctttta tgacaaatag aatttttatc gatcagtaat cgctgtcgct gcaaattgtt
      301 cgatcaaagg aatcaaagtc aatttgactt tgtatttccg ttcgagtgct ttttcgataa
      361 cgaactcatt ttcagtagtc atagaaatag ttgctaattt acgaggttca tcagcatttt
      421 tttcattaat aactacttca tgttcatttt gttctgtgta aaatttagga tactcagcaa
      481 cggcttttga aaaatcagca aaacttaaag cggcattcgt atcagttgag cgtgtatttt
      541 cagttgtgtc cgcttttggt ttttccgttt tttcttctgg cacactgact gtttcttctg
      601 taactggtgt cgcaacagct tgcgcagttg gttcatcgtt tttcaatcgt tgatcattcc
      661 agtaagacac gatttcaata agaagattgt cacggaatcg ttcgaaaatc ccgttttctc
      721 ctgaacgaat tctctttaat acagtattga agttgtcatc gattggcacg ttgatccgtg
      781 tatcttcatt tgttttccca gctaatccga tcgttaagat ttgacggttt ttgggatcaa
      841 gcatgacttg taacggcaaa ccataacgtc gcaagctctc ttcttctaga gaacggaata
      901 attgtcctct tacaccatcc aatgaaggac ggaattgatt taataatttt tgtttctgtt
      961 ttttcttcat atgttctctc cttcgctcat accctttcga ttataagaaa aaaacagcca
     1021 aatgaaaagg agtaatgcaa aaaaacttgc gtatacgcaa attacaacag aaaaatttgc
     1081 gtattttccc gttttttgaa agaaaaataa aaaatggaac ggattatgta tatatacata
     1141 atccgttcca ttctttctat tttttcggta tcaaggcaaa aacaggtagt tcaggtgaat
     1201 aaatgacttc atgttcgtgc cagggtttga ccattagcga atgatcgaac cgttttttca
     1261 gttcttcgat gatttgatca tgtgtccagt ttcttgcagg aaaatgttgg attttctctg
     1321 catcgatcaa aaagacaaaa tcggaatgaa aagtctgggt aacttcttcc gagagagaat
     1381 tcaagcgttc gattggatcc ataaaatcac ctttcgactt aaaattgggt agtatctggg
     1441 tcttttgctt tgccgactac accatctaac tgatcgattt gtttcatttc ttcttctgtt
     1501 aattcaaaat caaataattc agtattttct ttgattctgc ttggtgtcac tgacttagga
     1561 agaggaagga attcgtgttg cagcgaccaa cgcaaagcaa cttgtgcaat cgtcttgtca
     1621 tgtgcttcag cgatttgttt catttcaggc acatcgaaga ttttaccagt gcctaatgga
     1681 ctatatgctt cgaggatcat atcatgtttt tttgcatact caactacagt tgtctgtaat
     1741 tcgcctggtg caaggaaaac ttgattgacc atcggcataa tttttgctgt ttttgctagt
     1801 gtatctagat ggtggggtaa gaaattgctc acaccgattg ctttgatttt acctgcttca
     1861 tacagttctt caaatgctcg ccatgtatct gcatttgctt gttcccaatt atcacgaaaa
     1921 gcaacagggt ttggccagtg gatcaaaaac agatctaaat aatctgtttg gagcttcttc
     1981 aatgattctt caaaagaaga catcacaagt tcatagctgt ggtttgcatt ccataatttt
     2041 gttgtcaaga atatctcttc tctaggaatt ccactttcct taatcgcttg gccgacgctt
     2101 tcttcattct tgtatccttg ggcagtatcg atatggcgat aacctgcagc cagcgcttct
     2161 ttaacagagg aaacagcgac atccccatca ggtgtttgcc atgtgccgaa tcctactacc
     2221 gggatttcgt aaccatttga tagacgataa gtgcttgtta atgaattcat actttcagct
     2281 ccttttttat tcgttcaact ataagtgtag cgcatttttc gaaaaaaaga atccattttg
     2341 tttctactct tgaaattttt gtttaaaaga aaatgatttt gattctggta tttttgattt
     2401 tttttccgta aactaagaca gaagggaatg agaaaatgga tatctattta aaaaaagcaa
     2461 ttctgcatat tattgacaga gaaactggtt ctccagtctt ttcacaaaaa gagctggatc
     2521 tgacaaaaga atatattcgt gattttcttc aaaagaagat ccaaaaaatc tcttctgctc
     2581 aaacaaaaac tggacaacta gcagaagaaa gcatgatcgt tgaatcactt aaaatcgctc
     2641 aagaaaattt tgtggaagca agtgaagcaa tcgttcagcg gtggttcgat atctaccaag
     2701 aaagtgaaga agcacctagt gcagatgtat ttgttgtctt gtatgaatta gatacagtga
     2761 tgtacttatc tttattgaaa gtcaattatc gtgaggctta cactcacttt gttgaagcag
     2821 acgaagcagg gatcgacaac cgcttgattt tgaatcgagc aattctaggg gcgaaaacac
     2881 aaaaagcaga tgaagcattc gctgtgaatt tgactgattg gacctacgaa ttaattgaaa
     2941 aaaaatatga gttttctggc aaaaaagagc tttatttttc tggaagagtg atcgaaagtg
     3001 ttcctgcacc ttctttagaa gaaaatgtaa aagtgatcca aaaagtagcc aagcatcttg
     3061 gaaaaaaatt tgagacagaa gaatttgata tcatggctga tttgaaagaa gctgtctatg
     3121 atacgataga agaaaaaggt cggttggatc atgagatgat tgcggaaaaa gtcttcaagg
     3181 aaaacatcac ggcaaaaatg gcttttaaag aagaagtaca agagcaagga tttgtcccag
     3241 aagcacctcc tgtaaaagaa gtacaagaaa tttctgagaa aaaatttgga aaacaaaaat
     3301 tcaagctatc aaacgggatc gaactgatcg taccagtaga tgtatatcgc aacccagatt
     3361 tgattgaatt tgtgaataat ccggatggta cgatatccgt gatgatcaaa aatgtggatg
     3421 aagtattgaa tcgtttataa aaatgactag aatattaggt gttcaagtac cagtgatcag
     3481 ttaaatgaat tgtgtcaaga gaaggatcgg aaaagacgaa tataacaaaa aaagagcgtg
//
LOCUS       DAZKOU010000153         3470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000153 DAZKOU010000000
VERSION     DAZKOU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.160"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..319)
                     /locus_tag="ACG827_002801"
     CDS             complement(20..319)
                     /locus_tag="ACG827_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0484877.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(325..555)
                     /locus_tag="ACG827_002802"
     CDS             complement(325..555)
                     /locus_tag="ACG827_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484878.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(991..1557)
                     /locus_tag="ACG827_002803"
     CDS             complement(991..1557)
                     /locus_tag="ACG827_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0484879.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1557..2513)
                     /locus_tag="ACG827_002804"
     CDS             complement(1557..2513)
                     /locus_tag="ACG827_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0484880.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2513..3169)
                     /locus_tag="ACG827_002805"
     CDS             complement(2513..3169)
                     /locus_tag="ACG827_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0484881.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT         1101 a          671 c          596 g         1102 t
ORIGIN      
        1 cctttttagg tagcctactt taatctttgt atctttctgg atcaacgaaa gtatacttta
       61 tatagtcata acgccgatga tcgcttcgag cgtctagcac atcagtcacg atatcaaaca
      121 aaaaatatac gtctttcttc attctagttt tcgcagcagg gattttaaag tagttcttat
      181 tagaatagta gagattaatt aataagctat cttcgattgc taaaaagaaa acttctgaat
      241 cccacacctt ataaaaatct ttgacaaatc tattcgaagg atcaaattta aaccataatt
      301 gcgtctcatt aaaaagcata accattactc cgatctattt ttaaacttag tctctacctc
      361 taatatatat cgagttttta ttttgccttc agagaatact gtttcttttt ttgcagttac
      421 aggttgttta ttttcggaaa aagctaatat agctaaaatt gaaacatcca tctggaattt
      481 atctttttta ctgctttgat cataaaagtc tgcatattca tcactgatat tttttctaat
      541 aaattcttcc atcataaaaa tcacctcaaa acgattatac gaacttacgt tcttgttgta
      601 aagcgatatt tgaggggcaa aaaaggggca taatttgact gatattaagt tagctattga
      661 tatatgaatg acatacaaaa atataaaatc ttttaaaaat gcttatatat caatgttttt
      721 gccattcttt tatatatctg ttatatgtca aaaaaatgtg ttcaatttca atttgtattt
      781 gaataaagag gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caatcaaaaa
      841 cagcttctca tgtttttgat ttggttggac ttattaccgc agatataagc ttttgagcac
      901 cgtgaataaa gaggttatga aatcaacgct ctaacctgag tattagagaa aaaaacgcaa
      961 aacatatcat ttatgttttg cgttttttat ttatcttttt ttctcaatac aatataaaat
     1021 aggcggctgg ttcttttggt tgataaaccg ataattcagc acattatatg tttcctgtgg
     1081 cagtttggaa caaaaagaat caaccatatc cagttctttt tctccacctt catgaccata
     1141 gtagacgaca aggatcaatc gtccgcgagg cacaagtcgt ttcaatattt cttccatagc
     1201 tgtgcgtgta gtctctggca tagtgatgat tgctttatca ctttttggga gataccctag
     1261 attgaaaatc cctgctttga gattttgttg ttcagctatc acttctccta acgtttcgtg
     1321 gccttgtaga aacaatgaga cacgttcttc taactgacgc tgttctagcc tttcacgagt
     1381 agcatcgatt gcttgttgct ggatatcaaa actatagaca tgaccagttt ttccgatgtg
     1441 ttctgctaga aaaaccgtat cgtggccatt gcccatcgtt gcatctacga cgtgatcccc
     1501 tggttgcaag atttcttgca gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag
     1561 acgctcacct ttttccgtac atcatatttt ccttggaagc tatctctgcg aagaagttct
     1621 tcatcaattg cattcaagac ttcccatttt ttcaagctcc acattggtcc aatcaaggaa
     1681 tcccacggcg catcccctgt caaacgatgg atgatgatct cttttggtat catttccagc
     1741 tggtcgcaaa tgacattgac atagtcagga cggctcatta attgaaggcg accttcatga
     1801 tagtcccgca acattctcgt tttgcgcatc agatgcatca aatggagctt gattccttgg
     1861 atatctgaat ctaaaatggt gcgtctgaca ttttctttca tcatttctaa actttcgcct
     1921 ggtaatccgt tgatcaaatg cgtacaaaca cggatattat gtttgcgtaa tttagccacg
     1981 ccatctaaat aagtttgata atcatgtgct cggttgatca gttttgaagt ttcttcaaaa
     2041 gttgtttgca atccaagttc tacccataaa tacatccgct cgttcagttc agcaaggtaa
     2101 tcaactactt catctggcag acaatccggt cgtgtaccga ctgataatcc tacgacacct
     2161 ggtaaattga ctacttgctc aaatcgttct ttgattattt caactggcgc atgggtgttt
     2221 gtaaaatttt ggaaataaac gatgtattgt tggacatgag gccatttttg atgcatcatg
     2281 tcgatttctt tctggaattg gattggaaga gggtcttcag gtgcaacgat catgtctccc
     2341 gaaccagata cactgcaaaa agtacaaccg ccatgggcaa cagtcccatc tcgattcgga
     2401 cagtcaaatc ctccatcgat cggcaccttg aaaattttcc cgccaaactc atttcgtaaa
     2461 gcgtaattcc aagaatgata tcttttattt ccttctgaat aaggaaaagt cattaaaagt
     2521 ccttctttct gtaagagtta tctatttgtt ttcttttttc tctatacact ggataagtca
     2581 ggcacagcca agaaagactg agtaagtaac cgccgatgat atcagtagga aaatgaacgc
     2641 ccagataaat gcggcttatc ccgacactta ctataataaa catcatcagc aattgaagca
     2701 atgtgcgtaa ggttcttttt tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa
     2761 gaatcatact gcctgctgca tgtccactag ggaaactgta acttgtttca actactaagt
     2821 gcgccaatga aggacgctct cgcatgaaaa atagtttgat caccgggttg atgattccag
     2881 caattacaat aaatccagaa acgaaccaaa atgtttctgc cctttttttc ttataccaca
     2941 tccataaagc cgcacaagca aataaaatga tgacggtaag aggattaccg aatttagtca
     3001 taaagagttg gtagctgttc agatcaggat aagaagctct gacaatgcct gtaaaccact
     3061 gatcgaaacc agaaagccac gacagatgga actttacgat ccagcccaaa atgaaaaaca
     3121 ggacaagaaa gaagctacca gccacctgaa atgatgtttt atatttcata caattcctcc
     3181 tttgaagcaa tgacttatta tagcattttt ggtccagcga aaaaaacggt aaagagataa
     3241 aaccaatgta tgattcctct ttgaataacc aatattctta taaaaaaaat aactgtaatc
     3301 ggaaagacat tttttggtaa tgcaaatgaa atgttaggga aataaaatca gtgtttttcg
     3361 aaatcctttt aaacaagaac gtagacgtag gaagaacttg aaaatgaaca gaagttgttc
     3421 attacggtaa attacaatat gattacaaaa aacaacaaag ctcttaaata
//
LOCUS       DAZKOU010000154         3236 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000154 DAZKOU010000000
VERSION     DAZKOU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.162"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(554..2167)
                     /locus_tag="ACG827_002806"
     CDS             complement(554..2167)
                     /locus_tag="ACG827_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0484882.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="ACG827_002807"
     CDS             complement(2308..3216)
                     /locus_tag="ACG827_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0484883.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1040 a          556 c          572 g         1068 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatat
//
LOCUS       DAZKOU010000155         3200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000155 DAZKOU010000000
VERSION     DAZKOU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.163"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG827_002808"
     CDS             301..585
                     /locus_tag="ACG827_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484884.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG827_002809"
     CDS             588..1004
                     /locus_tag="ACG827_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484885.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG827_002810"
     CDS             complement(1141..1860)
                     /locus_tag="ACG827_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0484886.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACG827_002811"
     CDS             2522..2608
                     /locus_tag="ACG827_002811"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0484887.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..>3200)
                     /locus_tag="ACG827_002812"
     CDS             complement(2911..>3200)
                     /locus_tag="ACG827_002812"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484888.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt attttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DAZKOU010000156         3036 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000156 DAZKOU010000000
VERSION     DAZKOU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.164"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..959)
                     /gene="murB"
                     /locus_tag="ACG827_002813"
     CDS             complement(24..959)
                     /gene="murB"
                     /locus_tag="ACG827_002813"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0484889.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1032..1952)
                     /locus_tag="ACG827_002814"
     CDS             complement(1032..1952)
                     /locus_tag="ACG827_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0484890.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2065..2817
                     /locus_tag="ACG827_002815"
     CDS             2065..2817
                     /locus_tag="ACG827_002815"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0484891.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          855 a          612 c          526 g         1043 t
ORIGIN      
        1 cttattttaa gttccattac ttattaattg gctaaacttc tgcttgtttc tgatattgct
       61 tgtttttctc caatgatccg cacttctgtt tctaaagaca catcaaattt ttctttgatt
      121 acttcttgga tgtgcgcaat caattcaata taatctgtcg ctgtcgcatg atcgatattg
      181 acgatgaatc cagcatgttt ttcagagact tgtgcaccgc cccatttcag gccttgcaat
      241 cctgcttgtt ggatcagctg acctgtatag tgtcctactg ggcgcttaaa gacacttccg
      301 catgaagggt attctaatgg ctgttttgat tgacgcaatt cggtcaactc atccattctt
      361 gctttgattg cttcataatc ccctgtttgc aaactgaatc gagcttccaa tacgatagca
      421 cgtaattctt ggactttact gtgacgataa gaaaatacca tttcgtcttt tgtcaaagtt
      481 ttcaatgttc catcttcaag caaaagatcc acttcagcaa aaacatcttt gatctctccg
      541 ccatacgcac ctgcattcat ataaaccgct ccaccgacac ttcccgggat gccgcaagca
      601 aattcaaatc cagtcaatga ttctgccaaa gctgcataag tcgtatcaat cagtgaggca
      661 cctgcttccg caatgaccat cgttcctttg acatgaatct gtttcatttc agtcagcatg
      721 atgactacac cgcgaatccc gccatcttga acgatcagat tgcttgcatt tcctaatacc
      781 atccaaggta tatcatggat tcggcaataa tcgatgattt gctttacttc ttcttgtttt
      841 ttcgggaatg caaggacatc agctggtcca cctgttttcg taaacgtata gttcatcaat
      901 ggttcatcaa acaatatagt aatcgtggct aagctttcaa ctatttcttc tttattcact
      961 gaggattttc ctttcttttc tcaaacgtcc tcattattct atcacgctta atacaatctt
     1021 gccagtcttg ttcaaagttg atgacggtag tgatctttaa gcacttcctc acataattca
     1081 tgacttctta caacttcttt ttgtttaagc aattctgttt ccgatttacc taaacggatc
     1141 tgagaaaaga aggcctctac cattggctca aacccgcgtt tgactagcgt gtttgtccag
     1201 tcgccaaatt ctttcacttt ttttccatca gcatcttgaa tgacaagctg ggtcagatcg
     1261 tttacttcat aagtaccatt tttacttgtt acttgatatt tttctatatt agcgccactt
     1321 gctaaatcca tcgttaaaat agccgtttga ttttgtgttt ctaattgtaa aatggcatat
     1381 tccaaaaatt cctttgtttc tcttatctgg tattttgttc tcaaaatggg ttcatctaat
     1441 aaatacacag ctgtatcaat aagatgtaaa aacaaatcat aaaggacgaa ctctgtcgtt
     1501 tcttttgccg ctattcgatt tttttgcagg tggatcaacc gcttatctgg gatacgtttc
     1561 aattcttcta ccataggagc aaagcgccgg ttgaaaccga tcatcaaaat tatttggttc
     1621 ttatcagcta attcttgcaa ttcacgaact tcatttagat ggacactcag tggtttgtca
     1681 atataaacat ggataccatt ttctagacat tttttggcta attcataatg tacttttgtt
     1741 gctgcgtgga tcatacatgc ttcgattttt tcttctaaaa gctcatcgat cgtttcatat
     1801 agatgttgga acccgtactc tttttgaatt ctttcttttg tttctttatt tcttgttgca
     1861 aaataaaaat caagtgttcc ttgttttttc gcataagttg gtaaatatgc tttttctgcg
     1921 atattcccta caccgattac accaatcttc atctactttc ctcctggatc atttagttac
     1981 tttcatctta caccattttt tactaaaatg aggtacaata gtatcgcaaa ggtaaggaaa
     2041 ttaattcgaa ggagtgtgct tttattgaaa tttatctctt ggaacgtaaa cgggcttcgt
     2101 gccatcgtaa ataaaaattt tttagaagtt tttcatgagt tagacgccga ttttttctgt
     2161 ctgcaggaaa cgaaactcca agcaggacaa attgatttag atcttccagg ttattatcaa
     2221 tattggaatt acgcggaacg aaaaggctat tccggcacag cgatttttgc taaaaaacca
     2281 gcattgaatg ccacttacgg aatgggtatc gatatccatg atacagaagg ccgtctgatt
     2341 actttggaat attccgattt ctttcttgta acctgttaca cgccaaattc tcaaagcgaa
     2401 ttaaaacgtt tagattaccg attagaatgg gaagaagcct tttataatta tttggaaaat
     2461 ctaaaaaaac aaaaaccagt catcgtgtgt ggggatctaa atgtcgctca tcaaaagatt
     2521 gatttgaaaa actggaaaac gaatcaaaaa aatgctggat tcactccaga agaaagagcg
     2581 gctctttctc gactgttgga taacgggttt atcgatacat tccgttattt ttatccaacc
     2641 caggaaggtg tctactcttg gtggaactac cgattcaatt caaggaaaaa caatgctggt
     2701 tggcggatcg attacttttt gacgagcaaa gatttagagc caggactagc tgatgcaaag
     2761 atccatacgg acattatggg cagtgatcat tgtccagtag aattagattt gaaataaaga
     2821 ggttgtgaaa aagtcgttta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc
     2881 acattttgca taaatttggc ataataccga gaaactggct tttgaacact gtttatcgag
     2941 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
     3001 catatttttc gtttggttgg gcttattacc gcagat
//
LOCUS       DAZKOU010000157         3032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000157 DAZKOU010000000
VERSION     DAZKOU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.165"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..225
                     /gene="pssD"
                     /locus_tag="ACG827_002816"
     CDS             <1..225
                     /gene="pssD"
                     /locus_tag="ACG827_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0484892.1"
                     /translation="KPDLVICTGVLAMIPICLITKMMGKKLIYIESFAKVTSPTQTGK
                     LMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            242..721
                     /gene="pssE"
                     /locus_tag="ACG827_002817"
     CDS             242..721
                     /gene="pssE"
                     /locus_tag="ACG827_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0484893.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            741..1808
                     /locus_tag="ACG827_002818"
     CDS             741..1808
                     /locus_tag="ACG827_002818"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0484894.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            2376..>3032
                     /locus_tag="ACG827_002819"
     CDS             2376..>3032
                     /locus_tag="ACG827_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0484895.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     C"
BASE COUNT         1132 a          327 c          599 g          974 t
ORIGIN      
        1 aaaccggatc tcgtgatttg tacaggtgtt ttagctatga tccctatctg cttaataaca
       61 aaaatgatgg gaaaaaaact gatttatatt gaatcgtttg caaaagtaac cagccctact
      121 cagacaggaa aattgatgta tcattttgca gatcaatttt atgttcagtg ggaaactatg
      181 ctacaatttt atcctaatgc tatttatttg ggtgggatat attaaataat aggagaaaaa
      241 aatgatattt ataacattag gttcacaaaa atttcaattt gatagattgt taaaagctat
      301 tgatgattta attgaaaaag gagtaataat cgaaagtaca tttgcacaga gtggttatag
      361 tgattataaa ccactaaatt attcctataa agcatttcta aatagggatg agtttactga
      421 aactatgcaa aaagcagaaa tggtgattac tcatggaggt acaggtgcta taattggggc
      481 cgtaaaaaaa ggtaaaaggg tcattgctgt tcctcgattg gctaagtatg gtgagcatgt
      541 cgatgatcac caactgcagt taattagaca atttgatgag atgaatatta tctatccttg
      601 ttatgaatta gaaaatttag aagatgccgt taaagaaaca agagttcgga tgtttaacaa
      661 gtatgtatct aacacggaaa cgattattaa ttcgatagag gtattaatag aaaaaatata
      721 aatataagga gtaaagcgtt atgcaaagag tacttatgat tggtaatgac agctcggtaa
      781 aaggtggaat tacaagtata attacacaaa tgctcaattt taattgggaa aaaaagaata
      841 tcgagatgac ttttataccg agttataagg gtggaaatgc aatatttaaa tctttatatt
      901 ttatgtatgc atttgctaaa attataggta tctctttatt gagtaaacct aatattattc
      961 atatgcacat gtcgtacaga gggagtttta gtaggaaata tgtgctgaat aagtggttta
     1021 gattattaaa tattcctgta atcatccatc ttcatggatc ggaatttaaa aaatggtatg
     1081 atagtgtttc tgtaaaaaaa caaaatcaga ttcgcttgct gttaagagag tctgattcca
     1141 cgattgtact tggagaaaaa tggaagaaga taattaaaga aattgagcca aattgtaaaa
     1201 ctgttgttgt agaaaattct attgtagttc cagaatatac cataaagtgg aattcaaaaa
     1261 tgtgccgatt tctgtttatg ggagttttga taaagcgaaa aggtgtacac gatttggtag
     1321 aagcagttaa aaacattgca gaacaatatc cagaaaaaaa gtttattttt gagattgctg
     1381 gagcaggtga tgaagaaata tcgctcaaag aattgataga agaatataat ttgcaatcgt
     1441 attttgaatt tacaggttgg gttgtgggag aaaaaaagga ggagtgctat agaagatcaa
     1501 atgtgatgat tttaccgtcc tataacgagg gattgcctat atctattcta gaggcgattt
     1561 cttatggaat gcctgtaatt gctacagatg taggagatat ttcttcagca gtacgtgatg
     1621 gagaaaatgg atatttgttt caacctggtg acgtatcagc gttgacatct gcaatactaa
     1681 aaatcaatga taaagataaa tacgtaaaat tttcacgttg ttcgaaggat ttggcaataa
     1741 cgaaatttaa cgatggaaat ttctttgata gaattttaaa tctgtatcaa aaagggagca
     1801 aggagtgatg acaaggaaaa taaaaaatgt ttcgattatg taatgaactt attaagtgaa
     1861 taaaatagag agaaaaaata ttgaaataga cgatgtaaat ttttatccgt gtaagaattc
     1921 aaatgaatag tagtgtttta gtgcttgtaa tagagcaaga gtacaagcac ggtacattat
     1981 ataaaaacat taatgatatt tctataaata aatagatttt gtattctgga tgatgaagag
     2041 gaagatatta tctgataact taccctaaac tttgcagggg gtggttataa gttgtgtata
     2101 tgtggcagta atgaatgagt gaaataatca tattaagaaa taaaattgca atattgtgta
     2161 atacaaaagt atattttcat tattccaagg gaaagcatac ttacgaggat catattattg
     2221 atttaataaa taatttaaag gaaataattt ttctagaaga agatagtatg tcattcacat
     2281 catcaagatc agagtctatt tttttcagag aatttaagaa aatcgttgat gccgtcattg
     2341 actaattgag aaaacgtgag tgaaaggata gaaggatgaa ttcgagtgat ctaattagtg
     2401 taatcatacc tgtatataat gttgaaaagt atgtaggaag ttgtttggaa tcagtgtgta
     2461 atcaaacata taagaaccta gatattataa ttgtggatga tggatctaag gattcatcta
     2521 ataaaatatg tgaagactat gcattaaagg attcaaggat tcgtattatt cataaaaata
     2581 atgctggctt gggattggca agaaatacag gacttgaaca tgtaaaagga aaatatgttg
     2641 cttttattga ttctgatgat tttgttgcag ctaattatat tgaagcaatg ttaaatggta
     2701 taaaaaggag taatgcagac acatgttatg ctggatatta tgaatattat gatgataata
     2761 atattatttc aaagccagca agatatgata gtacgatatt tacaaataac gctataattg
     2821 aaaaagtgtt gcttggaatg attggcactg agccgagtta taaagtcgat tttatattgc
     2881 caatgtcagt ttggcatgcg ctgtattcat ctgagataat tcaacagcac aatatcaagt
     2941 ttccgtcaga aagggattat atatctgaag atatcatatt ccatatttca tatttgcaac
     3001 attcaaaaat aacaagtttt attaaagatt gt
//
LOCUS       DAZKOU010000158         2978 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000158 DAZKOU010000000
VERSION     DAZKOU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.166"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG827_002820"
     CDS             complement(37..1257)
                     /locus_tag="ACG827_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0484896.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG827_002821"
     CDS             complement(1277..1888)
                     /locus_tag="ACG827_002821"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0484897.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG827_002822"
     CDS             complement(1931..2863)
                     /locus_tag="ACG827_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0484898.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          815 a          623 c          516 g         1024 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttata
//
LOCUS       DAZKOU010000159         2929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000159 DAZKOU010000000
VERSION     DAZKOU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.167"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..981
                     /locus_tag="ACG827_002823"
     CDS             85..981
                     /locus_tag="ACG827_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0484899.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1073..1288
                     /locus_tag="ACG827_002824"
     CDS             1073..1288
                     /locus_tag="ACG827_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0484900.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1306..1578
                     /locus_tag="ACG827_002825"
     CDS             1306..1578
                     /locus_tag="ACG827_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0484901.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1580..2443
                     /locus_tag="ACG827_002826"
     CDS             1580..2443
                     /locus_tag="ACG827_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0484902.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1160 a          464 c          510 g          795 t
ORIGIN      
        1 gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa
       61 atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa agaatggggc
      121 gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat tttaaacaat
      181 aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg aaagtccaaa
      241 ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt aatgatcgat
      301 aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt agaattgcca
      361 cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat tgttcatttg
      421 actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc aaaattaact
      481 cgctcatgga cttttgaaaa tgaaagtaga ttgcttgcta ctcttttagc acctttaaaa
      541 agtgactatg atctcattat tattgatact gtaccaacgc caagcgttta tacaaataat
      601 gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga aagtacaaac
      661 aacattcaaa actatatttc ctatttgatt gatttacaag aacaatttaa ccctggacta
      721 gatatgatcg gttttgttcc ttatttagtt gatacggaca gcgcaacgat aaaatcaaac
      781 ctggaagaac tgtacaagca acataaagaa gataacttgg ttttccaaaa tattatcaag
      841 cgaagtaata aagtaagtac ctggtctaaa aatggaatta cagaacacaa aggctatgac
      901 aaaaaagttt tatccatgta tgagaacgta ttttttgaaa tgcttgagcg aattattcaa
      961 ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     1021 atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt gagtgattgt
     1081 gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg caaaaaaaga
     1141 tataatggga gataagacgg ttcgtgttcg tgctgactta caccatatta taaaaattga
     1201 aacagcaaaa aatggcggaa acgtaaaaga agttatggat caagccttag aagaatatat
     1261 acggaaatat ttacctgata aactttaaaa aggagtgaaa atgaaatggc agttacgtat
     1321 gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta taatcgagta
     1381 ttaaactatg ttttgaacca tgaactagat actaaaaata ctcgtttact agaagtgaat
     1441 cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc atttgaagaa
     1501 ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca aattttgaca
     1561 aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc aatttgaaga
     1621 tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg aatcgccaac
     1681 cgctttttta cttggtgggc aaccaggatc aggtaaaact agtttgcgat cggcaatttc
     1741 cgaagagaca caaggaaatg ttgttatcat tgataatgat acgttcaaac aacagcaccc
     1801 taattttgat gaactagtga aactttatga aaaagacgta gtaaaatacg ttacccctta
     1861 ttctaatcgc atgacagaag cgatcataag ccgtttgaga gataaagggt ataatttagt
     1921 gattgaaggt acaggacgaa caacagacgt tcctattcaa accgcaacaa tgcttcaagc
     1981 caaagattat gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact catatttagg
     2041 aacaattgaa cggtatgaaa ctatgtatgc agatgatcca atgacagcca gggcaacacc
     2101 aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa cccttcataa
     2161 aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac tctattcaag
     2221 cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa atcgtaaagt
     2281 atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg ttcaaaatca
     2341 acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct tgcagccacc
     2401 tacaccacca atacccaaaa cacctaaact tccagggatt taaaccttaa aaaagtaaaa
     2461 gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc tcatttttta
     2521 aaagctgatc gagaaataaa gcacacaact gtattctttc ttttttcagt tggtacattt
     2581 tgcagtctgt aacgtgccat ttactgtttg ttaccttaac atatccagtc gaaaacacaa
     2641 gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt gcgttctcgt
     2701 cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa aaagaaagga
     2761 gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt cacttcctca
     2821 aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaagaaaa gaggaaaaga
     2881 aaatggaaaa aacaaaaaaa ttacaattag aagattttac agaaaatgg
//
LOCUS       DAZKOU010000160         2899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000160 DAZKOU010000000
VERSION     DAZKOU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.168"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG827_002827"
     CDS             <1..1412
                     /locus_tag="ACG827_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0484903.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="ACG827_002828"
     CDS             1546..2889
                     /locus_tag="ACG827_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0484904.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1139 a          397 c          566 g          797 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaa
//
LOCUS       DAZKOU010000161         2605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000161 DAZKOU010000000
VERSION     DAZKOU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.169"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..2537
                     /locus_tag="ACG827_002829"
     CDS             96..2537
                     /locus_tag="ACG827_002829"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0484905.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          870 a          461 c          613 g          661 t
ORIGIN      
        1 gattgttaaa atttaatctt agaaaaaaat aactggaaca agagctattt gtttttacag
       61 caaagaatgg tatatttttg taggaggtgg cttacttgaa agaagtcgtt atgatagatg
      121 ctgcaagaac gccgatagga aagtatcgcg gaagtttaag tccatttact gcagttgaat
      181 taggcacact tgtgactaaa ggattattag ataaaacaaa actgaaaaaa gataaaatcg
      241 atcaagtaat atttggaaat gtcctgcaag ctggtaatgg tcaaaatgtt gccagacaga
      301 ttgctttgaa cagcggtctc cctgtagatg tcccagcaat gacgatcaat gaagtttgcg
      361 gatctggtat gaaagcagtg atcttagcac gccagctgat ccaattaggt gaagcagaac
      421 ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc tatgctgaaa cagtatcagt
      481 cagaaacaaa tgaatatggt gaaccaattt ccagtatggt caacgacgga ttgactgacg
      541 cattttcaaa tgcacatatg ggattaaccg cagagaatgt tgcaacacaa ttttctgtga
      601 gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt gaaagcagca catgctgtcg
      661 aagccggtgt attttctgag gagatcatcc cagtcaagat ttctgatgaa gacgtgttat
      721 ctgaggatga agcagttcgt ggaaatagta cattggaaaa actgggcacg ttacgtacag
      781 tattctcaga agaaggaact gtaacagcag gaaatgcttc cccgttgaat gacggtgcct
      841 ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa taatctgcct tatttagcaa
      901 ccatcaaaga agtagcggag gtcggtattg acccaagtat catgggaatc gctccaatca
      961 aagccattca aaaattgaca gatagatcag gaatgaattt atcaacaatc gatctttttg
     1021 agatcaatga agcatttgca gcttcttcta tcgtagttag ccaagaactg cagttagatg
     1081 aagaaaaggt caatatatat ggaggagcaa tcgcattagg ccatccaatt ggtgcaagcg
     1141 gtgcacgtat cttgactaca ttagcttacg gattgcttcg tgaacagaag agatacggta
     1201 ttgcttcatt atgtattggt ggaggtttag gcttggcagt cttgttagaa gcgaatatgg
     1261 agcaaacaca taaggatgtg caaaaaaaaa agttttatca gcttacgcca tctgaacggc
     1321 gcagtcaatt aatagaaaaa aatgtattga cacaagaaac tgcgttgatt tttcaggaac
     1381 aaacactttc tgaagagctc tccgatcaca tgatcgaaaa ccaagtgagt gaagtggaaa
     1441 tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa gaaaaaatgg attccgatgg
     1501 caacagagga accatctgtc attgcagctg caagtaatgg tgcaaaaatc tgtggaaata
     1561 tctgcgcaga aacacctcag cggctgatgc gagggcaaat cgttctatca ggaaaatcgg
     1621 agtatcaagc agtgatcaat gcagttaatc atcgaaaaga agagctgatt ctttgtgcaa
     1681 atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt tcaagatatc tctactcggg
     1741 aattcatggg gagtttccat gcatacttat ctattgattt tttagtggat gtaaaagatg
     1801 caatgggcgc taacatgatc aactcgattc ttgagagtgt ggcaaacaag ttacgggagt
     1861 ggttccctga agaagagatt ctattcagta ttttaagtaa ttttgctaca gaatcattgg
     1921 cttctgcctg ttgtgaaatc ccattcgaac gcttagggag aaacaaagaa atcggtgaac
     1981 aaattgccaa aaaaatccaa caggcagggg aatatgcgaa gcttgaccct taccgtgcag
     2041 caacgcacaa caaagggatc atgaatggta tagaagctgt agtggcagct acaggaaatg
     2101 atacacgtgc ggttagcgct tcgatccacg cctacgcggc aagaaatgga ttataccaag
     2161 gattaacaga ctggcagatc aaaggggata aactagttgg aaaactaacg gtgcctttgg
     2221 ctgttgccac agtaggcgga gcttccaata tcctgccaaa agcaaaagca tctttagcta
     2281 tgttagatat tgattcagcc aaggaactag cacaagtaat tgctgcagta gggttagctc
     2341 aaaacctggc tgctttgcgt gcattagtta cagaaggaat ccaaaaaggc cacatgggac
     2401 ttcaagctcg ttcattagcg atctcaattg gggctattgg tgaggaaatc gagcaagttg
     2461 cgaaaaaatt acgtgaagca gaaaagatga accagcagac agctatacaa atattggaaa
     2521 aaatccggga aaaataaaaa agcaatatct ttagaagaat aaagcaagat ttacagttat
     2581 tttatttact gcagatcttg ctttt
//
LOCUS       DAZKOU010000162         2590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000162 DAZKOU010000000
VERSION     DAZKOU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.170"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..1466
                     /locus_tag="ACG827_002830"
     CDS             63..1466
                     /locus_tag="ACG827_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0484906.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            1815..2447
                     /locus_tag="ACG827_002831"
     CDS             1815..2447
                     /locus_tag="ACG827_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0484907.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          976 a          368 c          505 g          741 t
ORIGIN      
        1 aaatatttat cataattttt aatagaaaac actattaaag agaaaaaaga gaggtaaatc
       61 aaatgaaaaa atctttaaaa cttgcatcag cagttatatt aatgtcccaa ctccttatag
      121 caacaccttt ccatgtgttg gcaagcgaaa attcaacata caaaattgca aaagaaagaa
      181 ccgagacgac tgaaatgagt aattccgatg taacagcaga gagtagtgaa cttttgacaa
      241 cttcatcaag tgaaagtagt acgacttcaa atgccgagtc ttcaacaact tcatcaagtg
      301 aaagtagtac gacttcaaat gccgagtctt caacaacttc gtcaagtgaa agtagtacga
      361 tttcaagtac cgaatcttca acaacttcaa gtagtacaag tgaaataact tcttcgaaag
      421 tgacaaaacc aaaccatata gaatcttcaa aaaaggaagc gccaaaaacg gaaggacaaa
      481 caaaaacttt ttcgacacca cagcaaatag aatcaacaga atctttccaa tcacaaccaa
      541 tactatctgg taatgaagaa attcattttg acaaaaatga atctgtagaa aattttatta
      601 tgaaaattgg tgagtctgcg agaaaaattg gtcagaaata tgatttatat gcttctgtta
      661 tgattgcaca agcgatctta gaatctgcca gtggtcaaag ccagttagct caggcaccga
      721 attacaattt atttgggata aaaggaactt ataatggaaa ctttgtcatt atggtaacaa
      781 acgaagattt gggtaatgga acgctttata cgacccaatc aaaatttaga gtgtatgaaa
      841 actatgagga aagctttgaa gattatgcca aattattaac taagggaatt agtggaaata
      901 aagattttta tgcaggagca ttaaaagcta atagtaaaac atatcgagaa gcaactaaat
      961 tccttacagg acggtatgca acagatacac aatactattt gaagctaaat gaattaatta
     1021 aaacctacga tttaaccaac tatgataaag aagttaaagg tccacaaatg agtaagactg
     1081 gataccaagt gcctattcat aattattcaa taaccagttt gtttggaaat cgtggaaatg
     1141 aatttcaccg aggacttgat atggcagcta cacagggtga gccgattcat gcagttaaag
     1201 ctggtaaggt tgttaaagca gagtttcact actcttgggg aaattacgtg gtgatacaac
     1261 acgaagatgg atcaaccgcg ttatatgctc accagcaaca atacctagta aaaactggag
     1321 ataatgtttt gcaaggacaa ataattggct atgtaggaag tactgggaat agtactggcc
     1381 cacaccttca tttggaaatt tgcaaagata gtagtctttc tcagagcatg ttagttgatc
     1441 cgaaaacctt attattcgga gactaaaaaa ggtaatcaat ctgacaaagt aacaagaaaa
     1501 tggttggatt cttagacctt tgatcaggat attgagactg gaattgatat ctcgattacg
     1561 atgggtttga caaataaaac aagtccatta tcgaatatcg agtgtattat tgccgtcttt
     1621 atgctcatat tttgagcagg tttcactaga tgaaaacaat ttatctatct tgaggatttc
     1681 gtgtacgtat cgttcagtct tgtattgtaa attagtcata aaatcagact aagaaaaatt
     1741 ggaaatattt ttttctgatt tattaaaagt taattgaaat tatatggtac ttctttcttg
     1801 ggagggtagg tgcagtgaag gaaagatatg gtcaccttac tgttgttgag gagattactc
     1861 ataatggata taaaaaatat cgttgcctat gtgactgtgg gaatgaaacg attgtatatc
     1921 gatcaaactt actatcaggt agaaccaaaa gttgtggatg tggaaattat aaaaataggc
     1981 ataaatataa aaatttgaca aataaaactt ttggtaagtt agttgcaatc aagccaacta
     2041 aactgcgaag tgaaggaact gttgtttggg aatgtcaatg tctgtgtggg agaaaaaagt
     2101 tagtatcggc aaagcgactt ctaaaaggtg atgtaaaaag ctgtggatgt ttgaaatcaa
     2161 aaaagcatca cattgaaaat aaaaaattcg gattacttac tgcaaagtat cctttggaca
     2221 ataactgttc atggaaaaca aaatgggttt gtcattgtga gtgtggcagt atgtgtgaga
     2281 taagttatag taacttattt tttggacata ctcaaagttg tggatgttta aaaaggcaag
     2341 aaacaaggac atgtgttgaa gggacagtaa tagagtgtct aacgtcaaaa ttaccgaaaa
     2401 ataatacaag tggtgttaag ggagtttatc gttctaaaga gaaatagatt gcgtatataa
     2461 ctttaaaaaa aaagaggtac tatttaggat cgtatgattc tattaagagt gcagctcaag
     2521 ctagaaaaga tgctgaaaat aaattgtttc aacctttgat tgacaaatat cgaggtaatc
     2581 attaattatt
//
LOCUS       DAZKOU010000163         2483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000163 DAZKOU010000000
VERSION     DAZKOU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.171"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            216..734
                     /locus_tag="ACG827_002832"
     CDS             216..734
                     /locus_tag="ACG827_002832"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0484908.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            862..1545
                     /locus_tag="ACG827_002833"
     CDS             862..1545
                     /locus_tag="ACG827_002833"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0484909.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1854..1927
                     /locus_tag="ACG827_002834"
     tRNA            1854..1927
                     /locus_tag="ACG827_002834"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1888..1890,aa:Arg,seq:tct)
     gene            2086..2445
                     /locus_tag="ACG827_002835"
     CDS             2086..2445
                     /locus_tag="ACG827_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0484910.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          855 a          367 c          488 g          773 t
ORIGIN      
        1 gacataaagg tattcctttc taaaacttgt aatgttaggg taaacgtttt ttaaactaac
       61 gtcaattatt tataataaaa aatgagataa agatgaggaa atagaattag aaacatataa
      121 ttattaaagg gaaacatttt tacagctata atttgaaaaa atttatcgtt attatatgat
      181 taagaaggat tgaataaaga aagaagttga aaacgatacc tgattacaaa aaaataactt
      241 ttataagttc tataacgttg attatatttt taattcttat tcttctcgtc gtcttggttc
      301 cccgaatttt tggtttgact ccttatatta ttgaggacaa agaaatgagt ccatattatg
      361 cacaaggaaa tctgatatat gtacgaccga aaaatacaaa tgagatttta gtcggagata
      421 ttattacata ttatgaaaat tcaggaaaaa aaatctcgac gcgtcgcgta atagccgttg
      481 aaaacaacca cgaagatttt tatacaaaag ctgatacaaa gacttatatt gagcctggag
      541 tagtaaaaaa gagaaatatt atcggtagtc ccatctttca gattccatat attggactag
      601 tcctttcaaa aacggcattt gcatggattc aggggttatt ctttacgatt gctttttgcc
      661 taacaggaat cacagcatgg aatacctttg tagagttgaa aaagaagcgg agacgaacac
      721 agaaattttt gtaagaatgt taaaaacata aatgtattcc agagatccaa cgactcttct
      781 gaacatgaaa cacgagaaaa cactcatgca gcttgtagat ttagtcagtt gttcgtataa
      841 tgtgtgaaga ggaggagtag tatgcgcaat gaaatgatgc atcgtcctgt agtcaaagaa
      901 gaactattgg attttatgcg tacccagcaa aaaaaattac cgggtgagct aggcaaactg
      961 gaagaagaag ctcatgtagc agaagtacca gttattcccc atgaaacagt tgtatttttg
     1021 aaatttttac ttggacagct tcagcctgaa cgaatcttgg agatcggagc agctatcggt
     1081 ttttcatcaa gtgtcatggc cacgacaatc ggagaaaacg ctcatgttac gacaattgat
     1141 cgtttcgatg tcatgatcga gaaagctaaa aaaaactatg aacgtctagg actgacagat
     1201 aaagtgactt tgcttgaagg tcaagcagca gatatcttgc cggagttgtc tggtccgtat
     1261 gactttattt ttatggatag tgcaaaatca aaatatattg aatttttacc tgaatgctta
     1321 cgtttgttaa gaaaaggtgg cgttttgatg gtagatgaca tcttccaagg aggaacgatc
     1381 ttgctcccag atgaagagat tccccgtggc aaacgagcta tccatcgtaa attaaatgaa
     1441 tttcttcgtg ttgtgatgga tcatcccgat ttgacatcga caatcttgcc attaggtgat
     1501 ggtgtgatct taatgacaaa ggaatcagaa acaatcgagt tgtgatacat gattatggca
     1561 gcctgtagat aatcgattga atcgagggct caattcgaac ttgatgtaaa agtttctata
     1621 tttgtaagtc aattaaagga tacaggaaat ctaaataaac aggagtagca tgaagtcaat
     1681 gtagctcctg ttttcaattg ataaccttag aacagctgaa ggagaatgtc tcgttttaaa
     1741 agattgattt agtagttggg ccaaaataat tcattatttg ctaattaaat aactatttcg
     1801 tattgacaga agcaatttaa ttatctataa ttattgatgt tccgctgatt cttgtcatag
     1861 tagctcagct ggatagagca tccgccttct aagcggacgg tcgggggttc gaatccctcc
     1921 tgtgacgcta aaaaaccgtt agaaactgag gtttctaacg gtttttcttt gttttttgtg
     1981 ccaatattgt ttgttttcaa aggcaaataa aataaaaaaa catccttatc gagtacaatt
     2041 ataaggagca aaaaaataaa actcatcaga aaagaggaag aagatatgac gatactatct
     2101 agaattttag tgactctagt tgcaattgaa tttttttata tcatgtatat cgaaacggtg
     2161 agaactgatt ctgacacgac aagtagggtt tttaaaatga gtaaagaaga gttaagcaga
     2221 aaatctgttc aaacattgtt taagaaccaa ggagtgtaca atggactatt aggagtggga
     2281 ctactttatg gagcttatct ttcttccgca tctaaagaaa tcacaagtat gctattaata
     2341 tctatttttt ttgttgcatt atatggcagt ttgactagcg atcgttggat tttactcaaa
     2401 caaggtgggc tagctctgtt ggcattgctt tctattttct tataaatata gaaaaaataa
     2461 gaatttatct aacaggaaac ttt
//
LOCUS       DAZKOU010000164         2475 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000164 DAZKOU010000000
VERSION     DAZKOU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.172"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..903
                     /locus_tag="ACG827_002836"
     CDS             <1..903
                     /locus_tag="ACG827_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0484911.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            916..1824
                     /locus_tag="ACG827_002837"
     CDS             916..1824
                     /locus_tag="ACG827_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0484912.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1824..2441
                     /locus_tag="ACG827_002838"
     CDS             1824..2441
                     /locus_tag="ACG827_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484913.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          859 a          345 c          555 g          716 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagatgga acttatggac atactggcgt tatccgaggc
      781 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
      841 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
      901 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
      961 tacgtgggga tgattcaagt tcgacaaggc aataaaaaca ctgaagttat ggaagctaca
     1021 attgtcgaaa atggtcttcc atatgattta tcagaatgta aggtatattt tgaatcagtt
     1081 gttggcggaa aatatccagt ccaattagaa acaagaattg tggatgctaa aaaagggaaa
     1141 atcaaatata tttttgataa atattccatg cagtgtttgc atcgacaaac agccaatttc
     1201 attatattta aaggagaaga cttgattgga acaactcaag acttctccta ttttgtcatc
     1261 aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat tgaagattta
     1321 attgctgata tgacagattt tattaatgaa aataaaggcg attttacgga ctggatgaat
     1381 gaaaggaaag aagaatttga tcgctggaga gaagaacagg agaatagttt ccaagattgg
     1441 agagaaggac aagaatcaga ttatttgtca tggtttgaat caatcaagga tattttgaag
     1501 tctatcgatc caggtggagt aatgttagcc gaattaatgg atgcacgtgt agatcttcaa
     1561 ggtgtacgcc atgaatcgat ttctgaacgt ttcctatccg atctaaatta tttgtatcaa
     1621 aaaatgaaag cagcactttt cacgattgaa tacggtgaaa ttgaagtgac tgatattctt
     1681 caagacgatc tcttttcaga taatcacgaa gtcgaaaaaa ttgaaacagt agaatttcca
     1741 attgaagaag gagctttgat tattgcaact gttgatgatc caaaacagaa tgttttcact
     1801 cttgagaaag ttggggtgat ttgatggtta aagtaaagag aatgatggag actgatgaat
     1861 ctggtgttaa acgtcaattt atgccaatta cacatgtttc tgcagtttta ggcttagaaa
     1921 aaatgatatc aggacaatca aaagttcttt cggttaatgg taaatatggt gcagtcattt
     1981 taacgaaagc tgatttagga ttagagaatg caattactga actaccttat gcaagcgaga
     2041 caagcgatgg tattcttact gctgaaatgt ttcaaaagat cgtaaatgga gaaggtggaa
     2101 catatatttt gccgattgct actcctgaac aattaggagg tataaaagtt ggggaactct
     2161 tagaagttac tgaagaaggt gttttatctg caactaagca aacagacttc aatttctctg
     2221 aagaattaaa gggaaaatta gaatctctaa aaatattaaa agcaggtgca aatatatcaa
     2281 ttgcagaaga tggaacaatt agttctacag gtggtagtgg gactggtggt gttaatcagt
     2341 catatgtaga tcaaaaattc caagaagctg taaatcaagc agaaaattac acaaacgaaa
     2401 gaattccaaa ttttactttt gaaaaaatcg gggaggtata gaaaatgaca gatatcgtaa
     2461 aaataaaaca aagcg
//
LOCUS       DAZKOU010000165         2459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000165 DAZKOU010000000
VERSION     DAZKOU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.173"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..1150
                     /locus_tag="ACG827_002839"
                     /pseudo
     CDS             6..1150
                     /locus_tag="ACG827_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="ACG827_002840"
     CDS             1321..1779
                     /locus_tag="ACG827_002840"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0484914.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="ACG827_002841"
     CDS             1938..2408
                     /locus_tag="ACG827_002841"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0484915.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          460 g          716 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgcctt
//
LOCUS       DAZKOU010000166         2405 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000166 DAZKOU010000000
VERSION     DAZKOU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.174"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..103)
                     /locus_tag="ACG827_002842"
     CDS             complement(<1..103)
                     /locus_tag="ACG827_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0484916.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(135..458)
                     /locus_tag="ACG827_002843"
     CDS             complement(135..458)
                     /locus_tag="ACG827_002843"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0484917.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            920..2302
                     /locus_tag="ACG827_002844"
                     /pseudo
     CDS             920..2302
                     /locus_tag="ACG827_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          718 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc ctttttttgt
     2341 aaaaaactat tgcacaatac aagtagccta cggctatcga aatttccata actttcgagc
     2401 aagaa
//
LOCUS       DAZKOU010000167         2400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000167 DAZKOU010000000
VERSION     DAZKOU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.175"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG827_002845"
     CDS             207..2069
                     /locus_tag="ACG827_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0484918.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACG827_002846"
     CDS             2069..>2400
                     /locus_tag="ACG827_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0484919.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZKOU010000168         2395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000168 DAZKOU010000000
VERSION     DAZKOU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.176"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG827_002847"
     CDS             316..570
                     /locus_tag="ACG827_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0484920.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG827_002848"
     CDS             571..888
                     /locus_tag="ACG827_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0484921.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG827_002849"
     CDS             1447..2382
                     /locus_tag="ACG827_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484922.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAZKOU010000169         2355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000169 DAZKOU010000000
VERSION     DAZKOU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.177"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACG827_002850"
     CDS             <1..566
                     /locus_tag="ACG827_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0484923.1"
                     /translation="RYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSA
                     FKSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKII
                     EIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYF
                     FENVVTGKENWNDLYHANEVLKIAEEK"
     gene            567..1319
                     /locus_tag="ACG827_002851"
     CDS             567..1319
                     /locus_tag="ACG827_002851"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0484924.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1324..2271
                     /locus_tag="ACG827_002852"
     CDS             1324..2271
                     /locus_tag="ACG827_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0484925.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          834 a          301 c          456 g          764 t
ORIGIN      
        1 tgaggtataa aaatatcatg gaagttgttc gtaatagtat tcaaagtaaa aaagtatatt
       61 ctgtaagagc tatttgttct acttatcttc ctgattggag aaaaggtact gattatagaa
      121 ataattattc tgcctttaaa tctatggggg gaggagtaga attagactgt atacatgaat
      181 tggattatat tataaaccta tttggtttcc ctaaatcagt aaaaaaacag tttggtaaat
      241 attcgaatct tgaaattaac agtaatgatt tagcaacata tatattgaga tacgatgata
      301 aaattataga aattcattta gattattttg gaagagttcc tacaagaaaa tttcaattgt
      361 ttactttaga tgaatgtatc aatgttgact tattaaataa taaaataagt tttgaagaaa
      421 acattgttaa agaaatgcct gaatctgcta atgatatgta tgagaaggag ttgaaatatt
      481 tctttgaaaa cgtagtaaca ggcaaagaaa actggaatga tttatatcat gcaaacgaag
      541 tattgaaaat agctgaggag aaataaatgg aaattttatt tactatttgt ggtagagctg
      601 ggtctaaggg gtttaaaaat aaaaatttga aatatttggc aggaaagccc ctagtccatt
      661 tcgctttagc aatgatagat atctatcaac aaaaacatcc tgaacataat tgcacgactt
      721 gtttgaatac ggacagtaat gagttagtag aattgacgac taagttaaat ccgtctgtga
      781 aaattgtaga acgtaaacct gaattagcta cagatactgt agggaaaatt gaagtgatac
      841 gtgattcatt cttacaacta gaaagagaag ataatatatt tgatttaatt atagatttag
      901 atttgacttc acctttacgt gaagtttcta atcttgaaga attaatagat gaacatctaa
      961 aaaatttaga taaagacgtc tacttttcag ttgtttctgc aagacgtaat ccctatttta
     1021 atatggtaaa aagaattgaa aatgaagtta ctatagttaa taaatcaaga tacacatcta
     1081 ggcaacagac acctgaagtt tttgaaatga atgcctcaat gtattctttt aaacctgaat
     1141 ttctaaaaaa aagtaattat atttttgatg ggcaatgtgg gataataaaa atgagagatt
     1201 atctaatttt agatattgat agtgaagaag attttaaatg gttagaattt ttgtttccta
     1261 aatttttaga agattcttcg gagcttcaag atatctataa ttatttatat cggaggtaaa
     1321 aggatgagct gttatcttat caccggtggg gctggcttca ttggttctac cttagccaat
     1381 catttaggag aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg aaaagtagaa
     1441 aatcttgata tgacgaaaaa tatcactttt attgagggag atgtcgctga gaaatccttg
     1501 atggaagaga tcatgaaagc taataaattt gattatatct ttcatttagc agcagttgct
     1561 agtgtggcag attcagttgc ccgaccagta gaaacacatc gtgtgaattt tgaaagtgta
     1621 ttaatgattt tagaattgat ccgcaagtat caacctgatt taaaacggat cgtgtttagt
     1681 tcttcagcgg ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga gtccgtgatt
     1741 cgcccattga cgccttatgc ggttgataaa ttcgctgcag aacaatatgt gctagattat
     1801 tgtcatttat atgatgtacc cggtagcgca gttcgttttt tcaatgtcta tggtccaaat
     1861 caaaatccga attctccata cagtggtgtg atttctattc tcgtagatcg ctataagaaa
     1921 caaatcgtag gagaagcaac ttcatttaca ttatttggtg atggaagcca gtctagagac
     1981 tttgtttata tagatgatgt aatccaagca ttacttttag ttgcgaatga ggaagctgca
     2041 ctggggaaac aattcaatgt tggaacgggt aaagcaacca cacttttgga attgattcat
     2101 gcaatcaatg aaattctcgg tgtagaattg acacttgaat acaaagaaga gcgttcgggt
     2161 gatatccatg actctctagc agatattact aagatccagt caattggcta tcaaccgaaa
     2221 tatggtattt taagtggaat gactaattat ttgaaaactg aaatcaatta aaacatacta
     2281 agataagatt agagggtaaa ttcaggctct ttgtcaataa ggactgatga gttttgtaaa
     2341 atcaaatctg ggata
//
LOCUS       DAZKOU010000170         2333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000170 DAZKOU010000000
VERSION     DAZKOU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.178"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(201..500)
                     /locus_tag="ACG827_002853"
     CDS             complement(201..500)
                     /locus_tag="ACG827_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0484926.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            851..1483
                     /locus_tag="ACG827_002854"
     CDS             851..1483
                     /locus_tag="ACG827_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0484927.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1606..2244
                     /locus_tag="ACG827_002855"
     CDS             1606..2244
                     /locus_tag="ACG827_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0484928.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          782 a          368 c          336 g          847 t
ORIGIN      
        1 ttcataaaat acacttttaa ttaacattat tagtttatac tagctgtttt tgtgttaatt
       61 aaaggataaa atacaatata aagaaacatc tgtttcttta tttagaataa aaaaatacag
      121 actctgttca aataaatgaa cctagcctgc attatgagat tgactatact ttttaattca
      181 ttgagatgaa taactcgttt ttataaatgc ccaaaagtaa tcggtgccga agccattcct
      241 cgacctaatt ctttttcaaa ttttcctatt gttgatatct ccatataaaa cggtgatact
      301 ggtttagaca attctcccct gattgctaac ggacgtaaac tacgttccaa attatttatg
      361 actttaggat aatactgacc tttttctaag cctgtctttg cgtctatcaa aagtatacgt
      421 tccttttctt tgatctctgg atttaaaacg agatcatata attttagtac catgttttct
      481 atttcttcac ttttagacat cacgatttcc tcccaaaaat atatttcttc ccactacatt
      541 ctataaacaa agtatacgct acatctagat ttttttaaat cattactgga ttgttaaaac
      601 ctttcaatac actgttatgg caacgatttt tattttaaaa acattctaaa ttaaatcagt
      661 aaaaattttt attctaagat ttttattaga attacgccta atttgtgtct ctcagggcaa
      721 ttctaagaac gtttcaagag atagcgacac tatcgttatc gaaaaaggcg aaaacctatc
      781 tcattttctg ctaatgtaag ttggtttagc ttttgttaat atgactttat gaaaatggag
      841 gtaattaaat atgatttttc ctaaagttaa tgaagcaagc ttgaataaag gatccttttt
      901 tcatatctat gaaaacgaag attttgaact gtataatatc ctttcacaaa aagaggtcat
      961 cgaaaaccaa ataatctgcc atccaattat ttacaaatct attttcaagt atgcattttt
     1021 tgcttccaat gattttcaaa gaatggagat taccgactgt atattcgaaa actgtgattt
     1081 ttcaggctgt atattgacaa aaagcatgtt gcatcgcgtg aaatttgtca attgtaaatt
     1141 aacaggtact aaattgatgg aaagttatat tgggaacacg ttattcgaga attgtaaaat
     1201 ggactatgta aatttatctg gttcaaacat aaaagaaagt acctttgagc actctgtctt
     1261 atcaagcgca gattttgtag actgttccct cactaaaata atgttttcta ctaatgattt
     1321 agaaaatgct agcttttttg atactgattt aaaaaatatc gatttaagta gcaactcctt
     1381 tgaaatgatt gaagtacgct cggaacatct aaaaaattgt acgcttaatc agttgcaagc
     1441 acttggtttt gctagaaaat ttttacaaat aaaagtagta taatcaaaat gaatatataa
     1501 gataaatttt tagcaataca aatatccata aaaaaacatt gcctatttta actcttcatg
     1561 tatactgact ctatcttcaa aaactgaata gtaggagtta ttatcatgaa taacatttta
     1621 ggaaaaaaaa tagaacttaa aaaagggata ttgggcacta ttgtcgttgt actcggtttg
     1681 ttagggttgc tatttgcgcc atccattatt agcatttttt ctttaactat cactggattt
     1741 atagcattta cacttgtttt tggtaaaaaa gatacgaaaa aaatgttctc gaaacctgtc
     1801 gctcctgtca aaaatatagc caaatatttt tttatcaatg tagtaatcag tgccgcagtc
     1861 tcggtatttt tgcaacaaat tttgaaatgg ggtttaacag gcaatccaat aaatgaggct
     1921 ttcagcccgt tattatttat tattttgcct atcatgatac ttggagaaga attgttctca
     1981 gtgtattttc tcgctatttt ttctagtaag ttcagcattc ctgtcgctag tttcttgtca
     2041 gcaatcatct ttggatttat ccattacagt acttatgata atggaaatat tttacacact
     2101 gtagcacaca tccttttgat acaaggggtg gcaagattgt tattcaacca agctgccatt
     2161 aaaagcaact cgatcattac aagttgggca gttcatgtga tatttgattt cacagctata
     2221 ttgctagtcg ttttattctc ctaaattttt atagaaataa tacatgctct attgctaaga
     2281 aagcatactt tatttccttt aaacaaaaag acagatgtat caatggatta aaa
//
LOCUS       DAZKOU010000171         2328 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000171 DAZKOU010000000
VERSION     DAZKOU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.179"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..>2328
                     /gene="acm"
                     /locus_tag="ACG827_002856"
     CDS             182..>2328
                     /gene="acm"
                     /locus_tag="ACG827_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0484929.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFL"
BASE COUNT          910 a          354 c          454 g          610 t
ORIGIN      
        1 attaggaaaa accttgtaaa aacagttttt tggcatattt attttttata aatatttgta
       61 aaataactat caatttttta gttaaaagat tggaaatgag tgggggagga attgaagaaa
      121 ggatttttga gagatgatat agtaggtttt atttttagtt aaaatagggg agatagaaaa
      181 aatgaaaaag tgtagaaata ttattttatc aatgttattt attattacta atataacatc
      241 actgattcct gttcatgtct atgcggatgc aggcagagat atcagcagta atgtcacttc
      301 gttgaccgtg gacccaacga atattacaga tggaggaaac ataaaagtaa aattctcgtt
      361 tgacgaaaaa aaacaaaata tccaaccagg tgattatctt tggatcaatt ggccaagtga
      421 aggaaatatt cgcggtgaag gattccaaaa agaaatccca ttaatgattg aaaataaaaa
      481 tgttggaact ttaacggtca gaaaagattc tgcgcaggtt gtgtttaatg aaaatattaa
      541 aaacctagac tcagtagaag gttgggggca atttgagata caggccagaa acgtaaccga
      601 taccggtgaa gaaaatacgg gaccattcac cgtgacgagc ggtgataaaa cagctactgt
      661 aaatgtgaca aagccagctt ctggttcttc ttctagcgtt ttttattata aaacaggaga
      721 catgctacct gaagatacta agcacattcg atggtttttg aatattaaca ataacggcac
      781 ttatgttgaa caaccagtta aaatcagtga tgaaatccaa agtggacaaa gattagatcc
      841 aagtacgttt gaaattaatc aaatacactt aggtgaacag aaggtttata gaggagaaga
      901 gggaatccaa caatttttac aagatttccc cagcgctaca tttaattttt cagtaactga
      961 taattacatt gaaataacta taccaaaaaa ctttgtcaat cttaggaaaa taatggtgag
     1021 ttataagacg atcatagaaa acccagaaca aataaatttt gagaatcatt cggaagcttg
     1081 gtttaaagaa tttaataaac ctgctgttga tggagaaagt ttcaatcata cagttaaaaa
     1141 tatttcagct tctggtggag tcaacggtac agttagaggc gaattgaaga ttttcaaata
     1201 tatcaatgac acagagattg gtatcccaaa tgtgactttc gaattaagac gagcagatga
     1261 acagcctatt caaggacagt ctagtatcct gttgacttcg aatgaacagg gagaagctag
     1321 tatcaaaggc ctgcaagtag gagactatgt tgtaaaagaa aaagaagcac ccaattggat
     1381 tgattttgat ccactaagtt caaatgaact caaattctcc ataaacgaaa atgatacaga
     1441 gggagtcagt ttacctattt ataacaagaa aaaggttacg aacatcacag caactaaaat
     1501 ttggaacggt ggaaccactc caagaccgag tatttatttt aaattattca gagcaagtac
     1561 aaacaattgg gaaccagttc ctgatgcaga aacaaaacga ttagacaatg gaataacttc
     1621 agtgacttgg gaagatatcc aacagtatga tgatagtgga aacgaatata cgtttaaagt
     1681 gcaagaagta gatcaaaacg gaaatgatta tgtcccttca ggttatcaaa aaattgaaaa
     1741 cgggctagtc gttacaaatg agaataaaga agtcatttca gtctcaggac aaaaaacatg
     1801 ggaagacaat gagaaccaag acggcaaacg accgacgacg atcacagtaa atctattagc
     1861 cgacggtcaa ccgatccaac acaaagaagt aagtgaaaaa gatgattggc gctatcgttt
     1921 cacaaatctg ccaaaataca gagacgggca agaaatcatc tatactgtaa cggaagacaa
     1981 cgtgcccgaa tacagtacga cgattgacgg gtataacatc aaaaacagtt atacaccgga
     2041 aaaaacaaat atatctatac caggtagcca tataacaccg tggaaaaatt ttccagagaa
     2101 gacagagaga gagaaaatta gaaatctatt ctctcaaaaa ttgcaaatat ttgctcatac
     2161 aggagaatcc agcgaacaaa gaagcaatta tattagtaag agagctgtac ctaaacagat
     2221 aacaaataaa tcaaaaagca ttcttcctaa aacaagtagt aaaaaatcta gttttgctgt
     2281 aatcattggt ttattgatag taactatctg tgctgggata ttcttaca
//
LOCUS       DAZKOU010000172         2301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000172 DAZKOU010000000
VERSION     DAZKOU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.180"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1365)
                     /locus_tag="ACG827_002857"
     CDS             complement(46..1365)
                     /locus_tag="ACG827_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0484930.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1362..2015)
                     /locus_tag="ACG827_002858"
     CDS             complement(1362..2015)
                     /locus_tag="ACG827_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0484931.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          761 a          359 c          195 g          986 t
ORIGIN      
        1 aataagtctc tatttttcaa taactattgc aaaattatat cttaattaag aaaatatttt
       61 tattttaaag ataaaaaatt cttcatcctg caatacttta tattctatat tgtaattatt
      121 cagaatatta cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa
      181 tttaacatca aatattttat ccatattcga aattttattg ttaccgtatg aattctctat
      241 atataaccaa tcattaacta tcccaatatt aattacccca tttacatcag tatattttac
      301 cgcattacta atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt
      361 ctcgtctaat atataattat tgacagttat tttcttttga tttgctaata tttcatactt
      421 ttttaatatg tcttctaatg tatcatttat tttcaaatat tcttcatcat tattcaaatt
      481 ttctatagaa tgaactgata atatttgaga aatatttttt gttaaactat caacgatttc
      541 aatacattca ttaatataaa tatctctttc tttatatttt ccaatattgt atttcatgtt
      601 ttccagtatt attttaagac tagcaagagg ggttttaagt tcatgggatg cacctttaaa
      661 aaagtcatat tttaactttt ctaattttaa aatttcttta tttttaaatt ccaaatcgtc
      721 aattgttctt aataaagtag aatacaaatc attaatttgt tgttttaatt ctccaacttc
      781 atcattagag ctaactttta aactcatttt tttatcaagt ttcatcattt tatcagtaac
      841 aatttttatt tcttgtatat tattttttat tgattttgca taaattaaag aaataatagc
      901 agaaaataaa attgatatta ataaagaata tggtaaaaat ttaaggctta aatctttcgc
      961 atctttttgc atatcagctg tagaaacaaa ttgtagattt gtttttttac catcattaag
     1021 ctttatttct ctttcttcaa ttatcaaaga attactatca ctttctaaat taacatttat
     1081 attatctttg atttgtattt cattcttatt atttttttct tttatgaata cttttatttc
     1141 actactcttg gaataaaact ccaaagtttg ttctatatat tttatttctt ttccattcat
     1201 attactagaa atttcatttg ccatattatg aatcttttct tttctcgtct ccaaatatgt
     1261 tttaggaaag ataaaaaaaa ttaatgaatg aactaatata attattattc caagaacaga
     1321 aaatatttgt atgaacattt ttggaaatat ctttaatttt ttcattttct ctccaattta
     1381 tatcctacat ttcttatagt agttatacag tctaattgta atttttttct aagttctttt
     1441 atatatacat ctattactcg atcataagga gtttcttcgc tatctttcca tacataatca
     1501 ataatttgca ttcttgttaa cacttgtcca ttattatcca ataaacattt taatacttct
     1561 agttcttttg catttacatc tatttcttca ccatttattt tagctgtata gctattaaaa
     1621 ttaaccgata gatccttata ttcaaacttc tctaaatgtc cataattctt cttaattaaa
     1681 gaatctattc tagcttttaa gaccggcaat gaaaatggct tttctacata tccgtctact
     1741 aaattagtaa atgcatcaat tttatattct tcatcactaa atgcagtcaa gattagaatt
     1801 ggtaaattgc ttttctttct aatttctttc aacacttcta aaccatttat aaaaggtatc
     1861 tgaatgtcta agataaccaa atttatatcg ctattaaatt ttgacaaggc ttctcttcca
     1921 tctttagctt gaataacagt atatccaaat tctgaaagat attcacttat tccctctctt
     1981 atcatattat catcttcaac agttaatatt ttcatatata cctccactta agcctataca
     2041 ctagatatta tacagcatat tatggctcta taatatctag tgttggtgac gccttctgtt
     2101 gccaatactt tttctttttt ttaataaatg caaacagaca ccttgaaatc ctttagaaaa
     2161 aaggcttcta taatattttg tgttgttgga aaatcgaagg cgattttcta ctaacacttt
     2221 ttcttcgttt caactcaaaa agagactcac ttttggtaaa atttagtcga ccaaaacatc
     2281 accctaccaa aggagtcgct c
//
LOCUS       DAZKOU010000173         2282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000173 DAZKOU010000000
VERSION     DAZKOU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.181"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1561
                     /locus_tag="ACG827_002859"
     CDS             <1..1561
                     /locus_tag="ACG827_002859"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0484932.1"
                     /translation="KIESLEPYDMIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMI
                     YQMGMEYAGLNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKY
                     FSIIGWIVFTLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLI
                     YAHQRNDVINVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQK
                     MYPDYTPVGELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQN
                     YYFIISAIMSIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFI
                     TLFQPFMIIWVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLIT
                     AMINIVLNIILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLI
                     SLCVIAFVASYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYI
                     LKVLTSVLVKR"
     gene            1739..1960
                     /locus_tag="ACG827_002860"
     CDS             1739..1960
                     /locus_tag="ACG827_002860"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0484933.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          772 a          239 c          435 g          836 t
ORIGIN      
        1 aaaaatagaa agtttggagc catatgatat gataattaat cgtaaacaaa atgctaaaag
       61 gaatataatt actggaacaa tttttaagtt atatcagatt atcattccct tttttatgcg
      121 tacacttatg atttatcaga tgggaatgga gtatgctgga ttgaacaacc tgttcacatc
      181 tatatttcaa atgttaaatt tagcggaatt gggaataggt gctgcactaa ctgtaagtat
      241 gtataaacct atagcagaag acaatagaaa agagatttgt gctttactga acgcatataa
      301 aaagtacttt tcaattattg gatggatagt gtttacatta ggattgttat gcattccttt
      361 tttgagattt ttagttaaag gttcgattcc tggaaattta aatatgacag ttttgtattt
      421 aatgtatttg ataaatactg ttttgtcgta ttggttattt tcatatagaa agagtctaat
      481 atatgcacat caaagaaatg atgtaattaa cgtgtctatg ctaattactt ttacattcca
      541 atttgtaatg caggcatatg ttctgatttt cttaaaaaat tactatttgt atttattggc
      601 ttctattttg ggacaaatag ttcttaactt aatgtgtgct gttgtttctc aaaagatgta
      661 tcctgattat actcctgttg gtgagttaga gaaagaaact attgacgata ttataaaaaa
      721 agtaaaagga ttagttacaa acaaggtagg cggtacaata ttacgatctt cagattcaat
      781 tattatttca tctttcttag gtctttcagt acttgcgatt tatcagaatt attactttat
      841 tatttctgcc ataatgagta tatttgcaat aatttatgaa tcatgtatag caggtatagg
      901 taacagtctt attgtagaaa gtcaagaaaa aaattatata gatttttgtg atattacaac
      961 aattataggt tggtttctgg caattagttg tgcatgcttt attacattat ttcagccgtt
     1021 catgattatc tgggtgggaa gtgataatct tatgagtatt tcatttgtta tatttatgat
     1081 agttcatttt ttcttatatg aaattgatca gttaattggg ttatacaaag atgctgctgg
     1141 aatatggtac gcagacaggt ttagaccatt aattacagca atgataaaca ttgtattaaa
     1201 tatcatcctc gttaattggt tcggattata tggagttttg gcggcaacca ttgtttcagt
     1261 attgttggtt gatttaccct ggttattata taatgttttc caaaatatat ttaaatttga
     1321 atcggcaggt aaatatgttt ggaagctcat aagtttgtgt gtaatagctt ttgttgcttc
     1381 ttatttaagt tattttgctt gttcgtatgt taatatcgga aatttcaaag tgataagttt
     1441 agtgataaat ttagttatat cggttgtcat atcgatttta gttattcttc tgtttaatat
     1501 ccgtaataag aagtttaagt atattttgaa agttcttacg tcggtattgg taaaaagatg
     1561 agaagagaca tcagaatgat atataaagta actacaattt ttaggctgcc gaataggcga
     1621 acgatgtata gtttttgttc caacgaagac tcttgttgaa attggagatg atatgaagga
     1681 gttacaattt tgactcatgt ttatgattga tcggttttaa aagtagtatg gagaagcttt
     1741 gggatcatcg gagaggatca aaataggtaa taatgttttt ataggaatga agtctagaat
     1801 actaaagggt gtacatatag gaaataatgt agttataggt gcaaatagtt tagtaaacaa
     1861 ggacgtatca gacgattgtg ttgttgcagg aaatctagca aaagtaatta tgtccctaga
     1921 tgaatattat aaaaagataa acaatatgaa gaatccgtaa aacttgtgaa actataccga
     1981 gaataatatg ggaaaaatat tgatgaaaaa gcattatatg agtttttagg gctgtttaca
     2041 gatggagatg acaatttgtc gaagagttgg gagcaatgca aaatttagta ggggattata
     2101 aacagactga caaggtattt aaagaacatg cacaagtatt tcataatatt gaagaatttt
     2161 tgaatactgt gagataggag ttgctacatt gagaaaagta tgtatttttc caatgtaaaa
     2221 gattacgagt ttgaaaacga caccataata ttatttttta agaagatatt atagagctta
     2281 gg
//
LOCUS       DAZKOU010000174         2255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000174 DAZKOU010000000
VERSION     DAZKOU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.182"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..510
                     /locus_tag="ACG827_002861"
     CDS             <1..510
                     /locus_tag="ACG827_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484934.1"
                     /translation="EIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSILTLAMAGCS
                     DSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRS
                     NRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDED
                     NPKTYDILA"
     gene            complement(571..936)
                     /locus_tag="ACG827_002862"
     CDS             complement(571..936)
                     /locus_tag="ACG827_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0484935.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1012..1257)
                     /locus_tag="ACG827_002863"
     CDS             complement(1012..1257)
                     /locus_tag="ACG827_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0484936.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1327..1581)
                     /locus_tag="ACG827_002864"
     CDS             complement(1327..1581)
                     /locus_tag="ACG827_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484937.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1745..2068)
                     /locus_tag="ACG827_002865"
     CDS             complement(1745..2068)
                     /locus_tag="ACG827_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484938.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          853 a          371 c          390 g          641 t
ORIGIN      
        1 gaaattatta attttcgtct gaaaacaaaa aaagaaagcg ttattatttt agtctatttt
       61 caggggggga accttatgat aaaaaaacta tttttactat caatattaac gctagcaatg
      121 gcaggttgtt ctgactctgg cttagcaact gaactgatca caaaatcaac tgaagccacg
      181 acagaggagt taaaatgtgg aaacgatcac agtgactaca gaggcttaaa tcgacgatgc
      241 atgggaatat taggcagaag caattgtaag catcgatgta gtaatcgaag aaggtgtaga
      301 agaagcaaca gaaaaatcaa aagaaagatc aaaagaagca atctgtgctg tgcatcgtat
      361 acagacgtcg aagatgcggt acttttaaac cacgtcagga gatgttcatt tatgtctagg
      421 gaagttggcg atgaaccaaa actagagtac gcttttagtt ttgcacgtga ggatgaagat
      481 aaccctaaaa cttacgacat tttagcttga tctaagatgg ttaaagcttt ttaatgaagt
      541 aagaggagat agaaggcttt tctagttata ttaggtaaga taaattcctt tttgctccaa
      601 atttgctaaa cattctgcta gataaacttc atcgtgttcc ggataaatcg gcacgttgcg
      661 ctctacagta ggtaaatcaa aaaatgcttc gcatgatttg ccacgataca taggattttt
      721 ccactctggt gctgggctgt aaccacgttt ttccatttca tccatgacaa gctgatgata
      781 ttggaataat ctataaggag aatagtgaaa aacataatcc acagtagcat gtttctttcc
      841 ccagccattt cctcttaatg cacagcattc ccgatgttgt cctaaaagct gttgacgagg
      901 aagctttgaa atcaattttt catgccataa tcgcatagaa atgtctcctt tatatataga
      961 ttgttaacct tagacgttaa atgaaaaaac cagcaccaag gctggaaaaa atcacttcag
     1021 tttgcttgct gtttctgagc gaccaattag gtaatcaacg gaaatatcgt aaaagtcagc
     1081 gatatagatt aattcaatga tgcttggaat ttcttctcct tcttcgtact tacgatattt
     1141 gaataaagga atacttaacg cgctagaaac agctacttga gataaagatc ggccagttct
     1201 taagaaattc agacgacaac gtaaagtatc cagtaaaata gcttcttcga tactcattta
     1261 atcacctttc attatagtag aatagttata atgactatag aaagctacac atgcaatcaa
     1321 tagtactcaa tgttttttat ttaaacgtat caaataaaga atgacgatca ataaagacaa
     1381 aaccagtcct gataattctt ctataaaaac actatcgggt tcaaagtagt tgtaattaaa
     1441 aactttccac ataaagtaga tagccggaag agtcaaaata gctaaataaa accgttcttt
     1501 gttccaattc ataagataaa caatacttgc gaccagtgcg accgcaggca cccaaaacaa
     1561 aactctcacc aacgtaatca tgtaacggac tcctcaacga ttcatataat aaaagataac
     1621 atgttattgc atacatcaca aatgaaatgc taggaaaata aaagaagaat agatcaatga
     1681 aaaatgaaaa agcagaacag ttttctgctg ataagcagga actgttccgc tgaatgtgtc
     1741 ttatctatcg agaaaaaatc aatggattga tatctaagta attcaataca tataaaaaga
     1801 ggacgatcgc attcactaaa aaggaaagaa tgatgatccc atagcgaatg gaaacatatt
     1861 taacaaatac ttttgtttga atacgggcat atctttttcc tttatagctg agcatcatga
     1921 caagaagcaa aagggtaatc acaccttgaa aaatcattcc aataaacaga attcctgagg
     1981 tttgataatt taattggaga aaataagctg ctgccgcaac taaatcgaat aaaatcgcaa
     2041 taaatggcaa aataatcact cctaacattg agaataaata aaagaataac attttttcta
     2101 aaataagcaa ataaaaatta aagtaacaaa atagttatga cacaagtaaa agaaataaga
     2161 ataacaatat tattttactt atattttgaa acttgtgaga gtttgtcatt cgtgctatag
     2221 ttgaaaagta acaaaaataa attttattta tgatg
//
LOCUS       DAZKOU010000175         2205 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000175 DAZKOU010000000
VERSION     DAZKOU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.183"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG827_002866"
     CDS             complement(<1..914)
                     /locus_tag="ACG827_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0484939.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG827_002867"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG827_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0484940.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2042..2176
                     /locus_tag="ACG827_002868"
     CDS             2042..2176
                     /locus_tag="ACG827_002868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484941.1"
                     /translation="MKDEWQKLEKEKTALIYEEIEMIHKIENIRTIPNYIEAKIFFHH
                     "
BASE COUNT          747 a          384 c          421 g          653 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtata
//
LOCUS       DAZKOU010000176         2174 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000176 DAZKOU010000000
VERSION     DAZKOU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.184"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG827_002869"
     CDS             278..787
                     /locus_tag="ACG827_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0484942.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG827_002870"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG827_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0484943.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
BASE COUNT          758 a          380 c          418 g          618 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca cata
//
LOCUS       DAZKOU010000177         2089 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000177 DAZKOU010000000
VERSION     DAZKOU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.185"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..848
                     /locus_tag="ACG827_002871"
     CDS             <1..848
                     /locus_tag="ACG827_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HFQ0484944.1"
                     /translation="TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEM
                     TDLWGIGSRMKKRLNQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAI
                     PPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTS
                     ILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQL
                     DLFSDPQKQIDEENLDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAG
                     GLKND"
     gene            841..1191
                     /locus_tag="ACG827_002872"
     CDS             841..1191
                     /locus_tag="ACG827_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484945.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1173..1358
                     /locus_tag="ACG827_002873"
     CDS             1173..1358
                     /locus_tag="ACG827_002873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484946.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
BASE COUNT          736 a          335 c          406 g          612 t
ORIGIN      
        1 ctacagtagg tgtcggagat aatcccttat tagctaaact tgctttagac aatgaagcaa
       61 aacataatga tggattcctt gctgaatgga cgtatcaaaa tgttcctgat aaagtatgga
      121 atattcctga aatgacagac ctttggggta ttggatctcg aatgaaaaaa agactcaacc
      181 aaattggtat catgagtatt aaagacttag ctaattggaa tccatacact attaagagcc
      241 atttaggtgt gattggctta cagctgtatt ttcatgcgaa cgggatcgat cgaacagata
      301 tagcgatccc accagaaccg acaaaggaaa aatcatatgg taattcacaa gtgttaccac
      361 gagattatac caggagaaat gaaatcgaac tggtggtaaa agaaatggcg gaacaagtag
      421 ctattcgtat caggcaacat aactgtaaaa caggttgtgt ccatttatac attggcacgt
      481 cgatcttaga aacgagacca ggtttttcac accagatgaa gatccctata acagataata
      541 caaaggaatt gcagaattac tgtcttttct tgtttgataa gtattatgaa ggacaagaag
      601 taaggcacgt tggaataacc tatagcaagc ttgtgtacac tgattcgctt cagctggatt
      661 tatttagtga tccacaaaag caaattgacg aagaaaattt agataaaatt attgacaaga
      721 ttcggcaaaa atatggtttt acgtcgattg tacatgcttc ttctatgtta gaaggcgctc
      781 gtagtattac tagaagtaca ttggttggtg gccatgcagg cggcgcagga gggcttaaaa
      841 atgattgatt ataatgatcg tggacgttta aagtgggcag gtttttattt atctgatcat
      901 actgaaaaaa ttgatgccga caatgaacaa cgagccaatc aaaatttggc aaaagaacaa
      961 atgacgactg aagaggttgc ggaagtattg aacgctgcag tcataaaaaa tagatcagta
     1021 tgtattcaaa aagaagaacg aaacgctgaa ggatactatc cagaagatat tgttagcaag
     1081 atcttaggta gtgatgagct ggggatctac gttggaaaaa acaaagttca ctacgacgaa
     1141 atacgtcata tcgaatttta ccaggataat aaatggggtc gattagaatg aacattatcc
     1201 agcaatatga gctaaagtat ataacctttg atcagctatc agaagagatt tggggttatg
     1261 gccagcgttt gataaatgag gttggtgttg aacgcttctc tttctatgta gaagctgcag
     1321 caggatacca taaatacgaa tactatattg gctattaaag tgcaataaaa ttatatttct
     1381 tttatgtttt acattttgta tcaattctga taaaattacc tataggtaat taaaaaatat
     1441 aaaaaaacta gttacaacct taggacgttg taactagctt gaagcatact agagctaact
     1501 aaaatagcac tagaaagcaa tgttaaaccc gtccaacaaa cttgatttaa cattgagacc
     1561 agcttattca gctaaatata gttgaataag cttcttttgt tttaaaaaaa caaaaggtgt
     1621 actacgaata acgcaagtaa aagaaatact attacaacca gtaggctaca agtaagtatt
     1681 actaaacatt gcgtagttag aacctttgct ctattgtcta aagccacaaa attagagatc
     1741 agctttatga tgctaagagc gagctgcatc catgcggaca taggttcttt tgacactatt
     1801 aattctcctt tttaggataa gggaaattgc tagtagtttt ttcattagca ccatccccct
     1861 tcatttgatt gagtctagtg ctgctgtgat agtttgctgt ttaatatgct tacattctta
     1921 ttataacata aatgaaacaa acacatggtt agaggtcgta tcaatcaaaa tagtgtctca
     1981 caaacgattt attacttact cctgctgact aacggactgt tcttcagtaa ctgtaactat
     2041 atgtttgtta aaaccaccat tacctgtaga taaaaaccaa tatatttac
//
LOCUS       DAZKOU010000178         2019 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000178 DAZKOU010000000
VERSION     DAZKOU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.186"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..570
                     /locus_tag="ACG827_002874"
     CDS             100..570
                     /locus_tag="ACG827_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0484947.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="ACG827_002875"
     CDS             792..1895
                     /locus_tag="ACG827_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0484948.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DAZKOU010000179         2003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000179 DAZKOU010000000
VERSION     DAZKOU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.187"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG827_002876"
     CDS             <1..378
                     /locus_tag="ACG827_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0484949.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG827_002877"
     CDS             426..1826
                     /locus_tag="ACG827_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0484950.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZKOU010000180         1993 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000180 DAZKOU010000000
VERSION     DAZKOU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.188"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..1240
                     /locus_tag="ACG827_002878"
     CDS             71..1240
                     /locus_tag="ACG827_002878"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0484951.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1507..>1993)
                     /locus_tag="ACG827_002879"
     CDS             complement(1507..>1993)
                     /locus_tag="ACG827_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484952.1"
                     /translation="LNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKD
                     QLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNA
                     TVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSH
                     E"
BASE COUNT          704 a          338 c          303 g          648 t
ORIGIN      
        1 tgttatcaag atacaagcta ttttagcttt attctttctg taaaaaagaa aggagactaa
       61 ataaactatt atgaaaaaag gaaaacatgc taaaaaaaga aaatattcat ttttaatgat
      121 cgttgtcgtg ttgtttagcc ttagtgcgtt ctctacagga actattcttg gatggcaatt
      181 ttacaataat gaacactctc agatagttgc ccatgctcaa tcagctcaaa aaaagttgat
      241 actgactacc aatgaacaac agctcgttga aaaaatcgat caactattaa gaaaaaatga
      301 ttatattggc tcaatttacg taaagaaaaa taatcgcatc atcatggaaa aaggctatgg
      361 ctatgcgaat aaactcacgc aacaaccaaa tgatccttct ctttattatc agattggttc
      421 ggttcaaaaa gcattgacta ctcttttgat tttaaaacaa gtcgagcgtg ggcacctttc
      481 tttagatact aagctggctc aattctatcc acaaattgct ggcagccagc aaattactat
      541 ccgtgacttg ctgtatatgc gctcaggctt gcaccgaact gcttctccaa ccataccgat
      601 gactgatgaa gaagtcgttc aatttgcgat tcagcactta aaatttacag attatcagac
      661 ctctcattat gattcattga actttaccat attgactgga attttgatca aattaacaaa
      721 tcaaccgtat gaaaaattat tgaccgatga tatcatcgaa cctttaggat tacagcatac
      781 aaaattttat gaccaagtca aaaattcatc caatcatgca ctatcctatc aaatgtctaa
      841 agaggatgat tactttaaac cactgacaga atctgaaaca gagatccata acgaattagg
      901 gactggtaat atcagtatgt ccgtctatga tttaaatacg ctgtttacac aagttttatc
      961 tggaaaacta gtttccaaag acttactctt ctcattatgg cgtgaaaatg cgaatggaca
     1021 tccatatagt ggtggggtat atagcggaga taattatatc ctgtcgcaag gaaatatcga
     1081 ctcttttgat acatctgcta tatttagcaa agattcacag gatgctatca tcatggaaag
     1141 caatataaaa gctgacaaaa ctatcaaact gcctactgct gatctaagaa accaaatcta
     1201 tgacctgatg gaaggaacta ctaagttggc aaatagctga tatgatgaaa agatagcaaa
     1261 tactacttct ttgaatagca tttgcttaaa aaaagtactc gactaaacgt aggaaagagt
     1321 gtgggacaaa tctaaaaatc aatttttgct acagacttta attccaaata aacagaacag
     1381 aaagaggtgt tcttctttct gttctgtcca aattattcta agatttctct ttaccaacat
     1441 tatttgacaa agggcctatt aaatctaaaa tagtatcttt agattgctaa aatatgtgct
     1501 agcaaatcat tcatggcttg ttcttatttt taattttatg caataaaaaa gaagaacaat
     1561 tagcgtgatt atagaaataa ataatgagat tttagaaaaa aatgtgtatg aatacctgat
     1621 tgttacttta tttattccac tttcactttt tactgtaaca aaaggacctc cttgttttag
     1681 tactgtagca ttttttgaat taacagtaac cgttaatcct ttataccgga atacaggtaa
     1741 aactattgtt ttgccttttg aagatttaaa cgtaaatgta atttcatctt tatttggaga
     1801 aaagctacta taaataactt gaccatcttc tgtaacttga tggtttttca aaagcaattg
     1861 atctttttta tagcctatag gtacataatc aaaattctca aattcttcaa gtttattttc
     1921 catttgttca ttagaataag ttccttgaaa attcatttct ttcaccatgt taactactga
     1981 tgaataattt aaa
//
LOCUS       DAZKOU010000181         1982 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000181 DAZKOU010000000
VERSION     DAZKOU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.189"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..185)
                     /locus_tag="ACG827_002880"
     CDS             complement(<1..185)
                     /locus_tag="ACG827_002880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484953.1"
                     /translation="MEREPKKITSKAYRCYCRFLTPDTEFDEYSDYINNTIKKEISFS
                     QEKGKRYFHRRINVNFK"
     gene            complement(856..1386)
                     /locus_tag="ACG827_002881"
     CDS             complement(856..1386)
                     /locus_tag="ACG827_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFQ0484954.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1383..>1982)
                     /locus_tag="ACG827_002882"
     CDS             complement(1383..>1982)
                     /locus_tag="ACG827_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0484955.1"
                     /translation="EIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAGEGYKRI
                     LMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIYKYNTTT
                     GTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSAIIEFLQ
                     LFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          642 a          384 c          224 g          732 t
ORIGIN      
        1 aatttaaaat taacattgat tctcctatga aaatatcttt tacctttctc ctgtgaaaag
       61 gaaatttcct tttttatagt gttattgata taatcagaat attcatcaaa ctctgtatct
      121 ggagttaaaa atctacagta acatctataa gctttgctag taatcttctt gggttctctc
      181 tccattgtca agcaacatca aaacacctac ttactattcg attcttttct gccaaaaacg
      241 agtcagcatc actcacaact tatgtgttgc tgactccgac tagtcctttg ttcaatatca
      301 acttattaaa cagatataat atttttcttt atttcgtata ctccaagact tccatctttc
      361 tttcatctta tctctgaaaa aaacacagca aagtgccctt aataaatata cattagaatt
      421 tgcaaatttt ttcatcttaa gatatatcct attcatattc tttacaatcc taatgggtga
      481 ttacaaatct atttagttca agttctacat tataaacacc tttcttgata gtatcatctg
      541 ctgcacaagc gatgtcgatc cacttattag gggatttacg tgctcaaaag tggctattcc
      601 ttttgggaag tccatccata ataatggtcc atggattttt ttagggtctt gtcgttcaaa
      661 tttctcaaaa actttattat acttccatag ttttataatt atttggtttc actttggctc
      721 gttcaaacac caacttgtat ttcttttcaa cgttcgaact ggagcccccc ctataatctc
      781 attattattt tcatgacttt ttactaccgt ggaattacaa gcaacaacaa tattatctgc
      841 taaatgaaca cctttctata tcacgctttt tgctccaatc caacaatgtt tacctataac
      901 tattttatta tttttattca aaatcctatg agtattcaat tcaaaaatcg tatgtgaatc
      961 ggtatccata aataatgtat cccacgatac tgtacagcca tctttgaatt gaatttcctt
     1021 aaagcaaatt atactcattt ttcctgtcga attaaacttt gacccaatat ctaaaactgc
     1081 atttttcctt atgtcaatat gagccccttg tgcgataaac gctggccctt gtattcttag
     1141 tattccttca atacttataa tcgtttcttc atttcccgca gtatttaata ctggaaagcc
     1201 caaataaact atttttccag attgtaatac tatctcaatt ttccctctcg atttgcgtgt
     1261 aatccgtact ttacgatgca ccaaaatagg taatctccac gcatctgaaa tatgaagaaa
     1321 tttataattg aagtatatcg ttttaggtaa atttaatata ttctttatta tcaatccgag
     1381 tctcatcttt caccatctct ttttgtaatc aaaattagtt tataaaggat tacacccaat
     1441 ataccgccca acgtattata tacaaaatcc gaaatttgaa acgttcctag cctcaaaaaa
     1501 agttgtaaaa attcaattat tgctgaaaaa taaaatgcaa tttttcctcc atatactatt
     1561 gaggaccata ttttgggttt ctttatattt tcatatttaa tagacattac catataaaca
     1621 tatggaataa aaagaataat attttctaaa cacgctgtag ataactcttt tgttccagtt
     1681 gttgtattat acttataaat ccaccagtct cccataacat cagataaagg atttaaccaa
     1741 attgttcgat tgattaatgt tgtaaataaa atcatcgcag tatagaatat caaagcacaa
     1801 acttttctat attttctatc cttctttaaa tgaagataaa gttccattag tatacgttta
     1861 tagccctcac ctgcagcaat gccatctgtt gcatacataa aatagaccag tcctaaaatt
     1921 gtcaataaaa cagcaaaccc aaagggttca tataatttat ataatatatc tgtaataatt
     1981 tc
//
LOCUS       DAZKOU010000182         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000182 DAZKOU010000000
VERSION     DAZKOU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.190"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG827_002883"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG827_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG827_002884"
     CDS             complement(728..1744)
                     /locus_tag="ACG827_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0484956.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKOU010000183         1906 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000183 DAZKOU010000000
VERSION     DAZKOU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.191"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..282)
                     /locus_tag="ACG827_002885"
     CDS             complement(25..282)
                     /locus_tag="ACG827_002885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484957.1"
                     /translation="MSTPSRGIKNTPVLTGVWVTDRLDFNWCQKLMSRKGDEILAIYG
                     YSRISTSNQDYKTQIQTLENAGAEIIFLENHWIIEHLWKYF"
     gene            complement(350..805)
                     /gene="tnpA"
                     /locus_tag="ACG827_002886"
     CDS             complement(350..805)
                     /gene="tnpA"
                     /locus_tag="ACG827_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0484958.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1048..1906
                     /locus_tag="ACG827_002887"
                     /pseudo
     CDS             1048..1906
                     /locus_tag="ACG827_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT          607 a          371 c          356 g          572 t
ORIGIN      
        1 acagatcatc aatacttacg taactcaaaa atacttccaa aggtgttcga taatccagtg
       61 attttctagg aagattattt ctgcgccagc attttctaat gtttgaattt gtgttttgta
      121 atcttggttg cttgtgctaa ttcttgaata gccgtaaatt gccaaaattt catcaccctt
      181 tctggacata agtttctgac accagttaaa atcaagtcta tcagtaaccc atacacccgt
      241 gaggacgggt gtatttttaa tccccctaga aggggtcgac actcgctgcc ccttaaaagg
      301 ggatttttaa aagaccgcca actgctcatc ttcactcact tacttctctt tattttttaa
      361 atggttctac gtactctctt ttacttataa tatatcgaat gcgatcatca gcttcttgtt
      421 cacggatgta tttttcattt gtcttttgat tcaatccgac tgtactcaca tagtatctct
      481 tcgaccaaaa ggatttgttt ccgtatttat atttgagatt tgcgtgctgc tcgtggatta
      541 gtaccgcact acgacctttc agatacccca taaaactgga tatacatatt tttggtggta
      601 ctcgaacaag catgtgaata tgatctagca ttgcatgtgc ttctatgatt tccacatctt
      661 tcatttcgca caacttccta agtatttttc caagatcttt tcgtaattgc ccatagatta
      721 cttttcgttg atattttggt gtaaaaataa tatgatactt acaattccaa cgagtatatg
      781 ctaaactttt atcgtcattt gacatgaaaa aactcctttc gatctgatga gcggttggta
      841 gccactcact agtatatcga aaggagtttt tatcgctaaa gatacttctt ccacgcgtag
      901 aaacgcgcgg ttttcttgtt tcgcacgctt tacgcacaaa actgactgaa gtcattaaga
      961 aaataaaagg gtctaaaact tttaagtttt gtaccgaccc ccaaaagtta gaccaaaaaa
     1021 tctaactttt ggaggtcggt attttttatg gctaagtata cttttgaact taaattaaag
     1081 atcgttcatg attacttgga tggtaaaggc ggaagtgatt atctggcaaa gaaatattcc
     1141 attaaggctc catcacaagt gaagcgctgg atcaatgcct atcaagaatt tggtgaagag
     1201 ggtctagtaa gaaagcgaca aaagaaaaaa tattctgttc aatttaagct ggatgcgata
     1261 gagttatatc taacaagtga gctgtcctat cgtgaagttg ccaacacact taacatgaac
     1321 aaccctaatt tgatcgctag ttggatgcgt caattccgtg agggtgggat cgatggactc
     1381 tcaaaaacga aaggatgtcc acctatcttg tctaagaaaa atgaacccaa aaataagaaa
     1441 aaatctacaa ctaaagcaac gtctaaagaa cgtgaacgca tcgaggagtt ggagaaacga
     1501 gtccgttccc ttcaaattga aaatgccttt ttaaaagaat tgaggaagct gcgaaaacag
     1561 gaagcccaac aacgacgaat gaagcaatcg cacgaatcat cgcaagcctc cgaggaccgt
     1621 ttaaattagt tgagcttcta gaaactttaa agttccctaa ggcaatcttt atgtactggc
     1681 aaaaacgatt ggaccgtaaa aatccagatc aagagattga agcggaaatg cttaaagttc
     1741 gggagaagca caaggattat ggctgtttac ggatgaccaa tgaactaaga aaccgcggtt
     1801 tctctatcaa taaaaagaaa gttcagcgcc tgatcaagaa gctaggaata aaagtaacga
     1861 catacacacg taaatcacgg cgctacaatt cttatcgagg acaagt
//
LOCUS       DAZKOU010000184         1827 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000184 DAZKOU010000000
VERSION     DAZKOU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.192"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1753
                     /locus_tag="ACG827_002888"
     CDS             275..1753
                     /locus_tag="ACG827_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484959.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          665 a          254 c          326 g          582 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaaggt aactaga
//
LOCUS       DAZKOU010000185         1819 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000185 DAZKOU010000000
VERSION     DAZKOU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.193"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..233
                     /locus_tag="ACG827_002889"
     CDS             <1..233
                     /locus_tag="ACG827_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484960.1"
                     /translation="GDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIFMIVIWFLNE
                     RSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(485..1057)
                     /locus_tag="ACG827_002890"
     CDS             complement(485..1057)
                     /locus_tag="ACG827_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0484961.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1073..1678)
                     /gene="fic"
                     /locus_tag="ACG827_002891"
     CDS             complement(1073..1678)
                     /gene="fic"
                     /locus_tag="ACG827_002891"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0484962.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          536 a          367 c          271 g          645 t
ORIGIN      
        1 aaggagactt gaatgccatt attactttaa tcgtagttat ggtaaaatca ataggtaata
       61 tcacaatagg aatgctgttt attatattag cagcagtgct gatttttatg atagttattt
      121 ggttcttaaa tgaaagatct aaagacaatc aacaccgtag ggatatggag ataaaatcaa
      181 tagagtcaat agattattta tcgataataa cactaaaaaa gccacgttcc taaacgtgac
      241 ttacgctgaa cgatatgtgt tgtttggtag acgaacaaat cgttcattat aaatatatga
      301 tggtatccta atatagtaag cgtggctagc atacctttaa ttactttacc ttttacagtt
      361 accatacaca ttttttgagc agaaatcaag taaataaaaa aatattatat ttcacaacat
      421 gctaatagac tcatatctag aacgcattaa gtatttacct gtctggttcg ctgagacttc
      481 ttttttaact ttcttcaatt ttcttcttta ttctaaaaag cgtactgcga ctgaaaccgg
      541 ttatagcttc aacttcctta tacgatttac ctgaagtaag cagttcatat gcgtgcctct
      601 tcttcggtgt gattgttgct ttcggacgtc cttctctgaa attaggatta ttctttcttg
      661 caaacatttt tccttcttgg gtacgttcca caatcatatc acgttccatt tctgctacac
      721 tcaacaacgt ccgtgtcacc attcgcccca tggacgtatt ttcaatcgtt cctagattca
      781 aaaccttgat cgttacgtta tcatcgagta aaggttcaat gatctctaat gcttctctcg
      841 tgttacgagc aaatcgatca agcttcgtaa caactatttc atctccaggc ttcagaccag
      901 ccaacagctg attgaatttt ggccgatccc gttttgtacc cgtaaatttt tctgaaaaaa
      961 tattcttctt ctctacgccg ttctcaagca actgttgaat ttgttctttt gtactttgag
     1021 aagcggttga gacacgtgca tatccatatt tcgtcatttg gtccacctac ttttacaaat
     1081 catctatcgt atactcagaa tacccttcat agaaatagct gacatcgatt cctttcatat
     1141 ataactcgcg atcgtcaatt ttatcagtca gagcattgga aattagatag cgtatttcca
     1201 aatcgtttat gggacttcgc tccattgcag aaagatagtc tgctttatcg attaagttcc
     1261 aatctacaac tttttgcaat tcttccttta aaatgagatc cagccaaatt ctagtacttc
     1321 ggccatttcc ttctcgaaat gggtgagcga tattcatctc cacatatttc ttgataatct
     1381 cttcaaaaga agtttgtggc atctgatcaa tatgctgtaa cgaatgttct aaatacataa
     1441 ctggtgcaaa tcgaaaattt ccttttgcaa tattgaccga tcggattttt ccggcaaaat
     1501 cgtaaatatc cgaaaataga aattcatgaa tttcagataa acctttaaat gtaccaactt
     1561 cgatttcatt tatttttcca caatcgtaca attctttcgc tttttgttta ctgagttttt
     1621 cttcaacctt agccaactca acttgattgg ttaatcccaa tttattctct aaaatcatgc
     1681 gaaatctcct taactttatg acataagttt atgaaacact ctcaacccca ttatatcgcg
     1741 attttgttta tgtgtcaaaa actattaagt ttctgacccc cgatagattt ttcaagtgtc
     1801 tctattttgc acagtctag
//
LOCUS       DAZKOU010000186         1816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000186 DAZKOU010000000
VERSION     DAZKOU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.194"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(226..1674)
                     /gene="cls"
                     /locus_tag="ACG827_002892"
     CDS             complement(226..1674)
                     /gene="cls"
                     /locus_tag="ACG827_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0484963.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT          547 a          390 c          300 g          579 t
ORIGIN      
        1 ctatctcatt aaaagtttct atgctatact ggctttgaaa tgtgtctctt ttaatagcat
       61 atattagctt atttcttgct ttccccaaaa aacaagagat accccaaagt taatttcgct
      121 catgcgagac gctttctcca tttctgacgt actctcagaa atggccccgt tagaaaagga
      181 cacgagatct ccatctgttg atggttctca tgtccttttt atgttttaaa ggagcggcga
      241 aatcaatctt gctaatcctt ctttcatctt gatgattatt cctcggtttt catacatttc
      301 ttttgtgaga tgcgtagaaa ttttcgagtc ttcaaaaaaa gcttttctta cacgtgaagc
      361 cattcgttca tcataaacca gtgtattgac ctcaaagtcc aagcgaaatg aacgaacatc
      421 gatatttgct gagccaactg aaacaatgcc accatcgata atcatcgtct ttgcatgaat
      481 aaatccattc tcatatgttt cgatccttgc tccatactcg atcagctctg cagcgaaaga
      541 atacgttgcc cagtaaacga gaagatgatc tggtttgtta ggtatttgga tatgaacttt
      601 tacgcctgaa agaagagcca acttcagtgc ttcatgaatc gatccatccg gtatgtaata
      661 aggcgtttgg atcaatatct cccgtttggc taacgaaatc attttcaaat aagtcatttt
      721 gatttgttca tgttcggtat ctggtccgct tgtcacgatt tgaacagcta tatcaccagt
      781 agtgtgaatg gacgggaaaa atttgggaga atagcctact tcttttgtat gctgagaatt
      841 ccagtccatc aaaaaacggt tttgcaaggt atacaccgct tctccgtaaa tacgcaaatg
      901 attgtctcgc caataaccaa atttctttac caatcctaga tattcatttc caacattgaa
      961 cccacctgta tatccgattt tcccatcaat aactacgatt ttacgatgat tgcgataatt
     1021 gatccgagga ttaatatacg gaacaaatag aggaaagaaa aatgcgactt ctccgcctgc
     1081 ttttttcaaa ttctgaaaaa agctggaaga aacttgtgtg gaaccccatg catcaagaag
     1141 gactctgact tttacccctc gtcttgccgc ctctgtcaat gcatctcgaa cttcttcacc
     1201 taatgcatca ctgcgataaa tataatactg gaagtggata tggtcttctg cctgcttgat
     1261 atcttcaatc agcgcatcga atttttcacg accatctgta aataaagtga tttcattttt
     1321 agtcgtatag agcgtacttt caaataccgt gagcatatag atcaattgtt tcacatctac
     1381 ttgtccagta ggcggatgag gataaagccc tcgcatcaat gcctgtttgt cttcttcaat
     1441 ttccaggttc ttaccgattt tcccttggat cttcaaatca aagattttct ctttggaaat
     1501 ccctcttcct aaaaaaatat ataaaataaa tcctacaact ggaatgaaca tcaaaacgag
     1561 cagccatgcc catgtttgag cagtttcctt tctttctcga aaaacgatga taaaggaaag
     1621 caaaatattc ataatcaaaa ggatcgtaaa aaaattgtca acgataattt tcacggcttc
     1681 acctcttctt ctaccaactt aagaataccc aagttgtttt ttaaagtaaa gggaaaaaag
     1741 ccgcttcctt ctattctcct agccaataaa aaaagaaagc tggaacgaaa ctgtccaact
     1801 ttctcatttt atttat
//
LOCUS       DAZKOU010000187         1731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000187 DAZKOU010000000
VERSION     DAZKOU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.195"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1508)
                     /locus_tag="ACG827_002893"
     CDS             complement(27..1508)
                     /locus_tag="ACG827_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0484964.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          537 a          233 c          300 g          661 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
     1321 tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt
     1381 cggatcagtt ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa
     1441 gaacaattat ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca
     1501 attagcataa atacctcctt tttattctgt ttttgtatat agttagacaa aaactgtgag
     1561 tggaaagtca tttcagtctg tgttattctc acagagtgtc gccttctttt ttatataacg
     1621 ttatttaatt tttttgttac cttgtttatt ctattgttta tttcctaggg tattttgtca
     1681 agatattctt gtttttaagg cggaaaaatt gatttttaat ggatataact a
//
LOCUS       DAZKOU010000188         1664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000188 DAZKOU010000000
VERSION     DAZKOU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.196"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG827_002894"
     CDS             131..505
                     /locus_tag="ACG827_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0484965.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG827_002895"
     CDS             complement(619..1248)
                     /locus_tag="ACG827_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0484966.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG827_002896"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG827_002896"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZKOU010000189         1644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000189 DAZKOU010000000
VERSION     DAZKOU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.197"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..1595
                     /locus_tag="ACG827_002897"
     CDS             129..1595
                     /locus_tag="ACG827_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0484967.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          460 a          296 c          337 g          551 t
ORIGIN      
        1 tattgccatt aaaaattact tgcttttata gaaaattagt ttatatttct taaataagtt
       61 ttttagttaa atttttcggt aaagatgtat aatattttct gaaaatttaa agaattgggg
      121 tatagattat ggaaaaacag caagacaagg gcttcttcgg gcagccgaag ggattgtcaa
      181 ctttgttctt cactgagatg tgggaacgat ttagttatta cggtatgcgt gcattactta
      241 tctattatat gtacgataca gttgccaacg ggggacttgg attaccacga gcaacagcat
      301 tagctatcat gtcgatctac ggatctttga tctacatgtc tagtatcgtc ggcggatggg
      361 tatctgaccg agtgttagga gcaaaacgca ctgtattctt cggtggagtt ttcattatgc
      421 ttggccacat cgtcttggct ttaccatttg gtgtaagtac attgtttgtt tcgatggcat
      481 tgattattat cggaaccgcc tttttgaaac caaatgtttc tggcatggtc ggacatcttt
      541 attcaaagac tgacttgcga agagatgcag gattttcgat tttttacatg ggtgtcaact
      601 taggggcatt attagcacca ctaatcgttg gtacaatcgg tcaagaatat aactatcatt
      661 tagggttctc tttagctgct attgggatgt tctttggatt attacaatac atcatccaag
      721 gacgcaagac attagacgga gtgggtacaa caccgccgaa tcctttgaca actgaagaac
      781 gtaaaggatt catccgtaac ttgatcatcg cacttgttgt tatcgctgca gtattcggcg
      841 gggcacaact aactggtcat ctgacaattg acttttttgt caatacaatc agtgtattgg
      901 ggattatctt accgttgtat tactttatca aaatgctgac ttcaaaagat gtaactgctg
      961 aagaaaaacc aagagtatgg gcttatatcc cattattcct tgcagcaatc attttctggt
     1021 cattacaaga acaaggttca tctatcttag ctttatttgc tagtgaacgt acacaatcta
     1081 cgctatttgg tgtaacgatc caagcaagct ggtatcaatc attgaatcca attttcatct
     1141 ttattctgac accagtattc gtaacgatgt ggacgaaact tggtgaacgt caaccatcta
     1201 cagtagtgaa attctctctt ggacttttat ttgcaggatt atcgtacttg ctattgatgt
     1261 tgccgggtat gctttatggt acaagtggaa aagtaagtcc attgtggttg ttgggaagtt
     1321 tcttcatcgt ggttattgct gaattgtgtc tttcaccagt tggtttgtct gtgacaacaa
     1381 aattagcacc acgagcattt gaatcacaaa caatcgctat ttggttctta gctgatgctt
     1441 cttcacaagc aatcaatgca caaattgcac gcttctacac accgggaaca gaatctgctt
     1501 acttcggtat tatcggtgca gtagcaatcg tcggtggtat cttgctattt actgtgaaag
     1561 aaccaatcaa aaaactaatg ggctctattc attaatcaat gaacataaaa acaggaattg
     1621 cttatttgca gcagttcctg tttt
//
LOCUS       DAZKOU010000190         1636 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000190 DAZKOU010000000
VERSION     DAZKOU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.198"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..1502)
                     /locus_tag="ACG827_002898"
     CDS             complement(183..1502)
                     /locus_tag="ACG827_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0484968.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          525 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DAZKOU010000191         1607 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000191 DAZKOU010000000
VERSION     DAZKOU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.199"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..305)
                     /locus_tag="ACG827_002899"
     CDS             complement(18..305)
                     /locus_tag="ACG827_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0484969.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(295..624)
                     /locus_tag="ACG827_002900"
     CDS             complement(295..624)
                     /locus_tag="ACG827_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0484970.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(701..973)
                     /locus_tag="ACG827_002901"
     CDS             complement(701..973)
                     /locus_tag="ACG827_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0484971.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(973..1236)
                     /locus_tag="ACG827_002902"
     CDS             complement(973..1236)
                     /locus_tag="ACG827_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0484972.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            1470..>1607
                     /locus_tag="ACG827_002903"
     CDS             1470..>1607
                     /locus_tag="ACG827_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0484973.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          513 a          313 c          200 g          581 t
ORIGIN      
        1 agaacgacaa caaatcacta gattaatttt ttcaactgtt cataaaagtt ttcatgcgtt
       61 ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac tctatatgtt
      121 gtgcccgcat atttgaaccc actagaccaa atacctgata aatccccagt tttttgttca
      181 cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt ttctttcaat
      241 ggcttctctt ttagcttttt taaatactta atgacatgtt ttgaaggaac gatttcataa
      301 gccaatttct gttgccaact cctctttagt cattacttta gaattagtaa gtgtatcttc
      361 agcaatatct ctaaaggaag aatgcatttt gttttttcta ttcttaaatt ctacaagaat
      421 ctcatcttta tcgtagcctt ctgcaattat atcagacaaa atatcttcgc taaaatcata
      481 aaattctttt tgtggttcaa caaattcata aagaatacca ttctctacca actttgcttc
      541 aacttcgact ccattaggaa cattaaaatc tttaggtact gtcaacatga ttgaattgcc
      601 ttgttttctt gtttttgtaa tcatttaaat ccctcatttc taacagtatg taattactta
      661 gtaaatacat tataccacga gataatgtat ttacaagtaa ttactgtgac tcgtaatgcg
      721 tccttgctga ataaatttca accacgtgtt catcttcatc taccttataa acaacccggt
      781 gttgtctatt caatcttcta gaatacctac cttcatgtgt aggttttaac ttctcaaatg
      841 attgggtagg gagataggga tcttccttta aagtctgaat aaccttctca aattgaggtt
      901 tcaaattaga ctgtttgatt ttctttaaat cagctttagc cgagttttta atcatcacag
      961 aatagttatt cattataagt tatcccaatc aatttcatca acattagtaa atccactatc
     1021 atctttttct cgcttacgaa caacacccaa agtgcctatt tgctcaagta atagcgtttc
     1081 tttgattgct ttccaatcat ccaaaccaat aagcaccgca ttattttcac tgcgctcact
     1141 aatgatttca atttccgtat gattctcatt tacatctttc aacaacttat aaaaattttt
     1201 ccgtgctgag ctaggattta agactgtttt tgccatatct actccccctc taatttatat
     1261 ctccattcta acgtacttaa ttaagtacgt aaattcgatt gttttgttta aattggagta
     1321 ccaaaacagc attgtaaagt gttatccgtt tcctttatac ccgtggtgct agttaatttt
     1381 ttcctagagg aaaatgtcta aacatgttgg taaatcaaca aaaaaacgca tagagtagtc
     1441 tcaaaactcc caagaaatga ggactacata tgcgttcatc aaaatatact gaacattata
     1501 cagatacttt tataacattc aaggaaatta tgagaactgt ttctaacata taccacaatt
     1561 gtgtaccaga taagattaaa aaccgaagaa atactgatca actgaag
//
LOCUS       DAZKOU010000192         1536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000192 DAZKOU010000000
VERSION     DAZKOU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.200"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG827_002904"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG827_002904"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZKOU010000193         1482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000193 DAZKOU010000000
VERSION     DAZKOU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.201"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACG827_002905"
     CDS             <1..828
                     /locus_tag="ACG827_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0484974.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETKIVDA
                     KKGKINYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFERWRQEQEHSFQDWREGQESDYLAWF
                     ESIKDILKTIDPGGVMLAELTDARVDIQGVRHASISERLLADMDYLYQKLRATLFTIE
                     YGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1448
                     /locus_tag="ACG827_002906"
     CDS             828..1448
                     /locus_tag="ACG827_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484975.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          529 a          212 c          337 g          404 t
ORIGIN      
        1 aatacagagg ttatggaagc gactatagtt gaaaatggtc ttccctacga tttatcagaa
       61 tgtaaggtat attttgaatc agttgtaggt gggaaatatc cagtccaatt agaaacaaaa
      121 attgtggatg ctaaaaaagg gaaaattaac tatatttttg ataaatattc catgcagtgt
      181 ttacatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cttattttgt cattaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caatcgaaga tttaattgcg gatatgacgg actttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa
      421 caggaacaca gtttccaaga ttggagagaa ggacaagaat cagactattt ggcatggttt
      481 gaatcaatca aggatatttt aaagacgatt gatccaggtg gagtcatgtt agccgaacta
      541 acggatgcgc gagtagatat tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg
      601 gcagatatgg actatttata tcagaaattg cgagcaacgc ttttcacgat tgaatacggg
      661 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca tgaagtcgaa
      721 aaagttggaa ctgtagcatt cccgattgaa gaaggagcct tgatcatcgc aaccgttgat
      781 gatccaaagc aaaatgtgtt cactcttgag aaagttgggg tgatctaatg gctaaaacta
      841 aacgaatgat ggaaacggat gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac
      901 atgcttccgc agttcttggg ttagaagaaa taatagcagg agaagcaaca gttttatctg
      961 ttaatggaaa aattggagcc gtcgtcatta ctaaagaaga tttgggatta gagaatgttc
     1021 tcacagaatt accctatgca agtgaagaag atgacggcat tatcacggca gaaatgtatc
     1081 aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc agttgccact atcgaccgtt
     1141 tgggtggcat aaaagttggt gaattattga cgattgatga gacagggaaa gtctctgcag
     1201 tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa
     1261 attatactgc tggagaaaac atcactattg atgaagatgg aacaattagt tctacaggtg
     1321 gtagtgggac tggtggtgtc aatcagtcat atgtagatca aaaattccaa gaagctgtaa
     1381 atcaagcaga aaattacaca aacgaaagaa ttccaaattt tacttttgaa aaaattgggg
     1441 aggtatagag aatgacagat atcgtaaaaa taaaacaaag ca
//
LOCUS       DAZKOU010000194         1465 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000194 DAZKOU010000000
VERSION     DAZKOU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.202"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..490
                     /locus_tag="ACG827_002907"
     CDS             80..490
                     /locus_tag="ACG827_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0484976.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            504..860
                     /locus_tag="ACG827_002908"
     CDS             504..860
                     /locus_tag="ACG827_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0484977.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          540 a          211 c          271 g          443 t
ORIGIN      
        1 ttatatttta ttagctaata attggtattt taaaagaaat tactgtagaa ttggattata
       61 attaaaaagg agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag
      121 aaaagcaaga gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc
      181 cgaaccgtta acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca
      241 agatattatt tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt
      301 aactttaaag caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat
      361 tttgattgat gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt
      421 ccctcgtgaa attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt
      481 aaattcatag ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa
      541 caagtctgaa ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact
      601 tagaagaacg cgatctaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc
      661 aagcagaaca gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt
      721 cctgtgtagg aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac
      781 gtcctttgat tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac
      841 agcttatctt aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca
      901 gttgtaacag tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt
      961 ttctactata gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata
     1021 gagttaaaaa tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa
     1081 gtagcaaagc aataaaatag tattttaaaa aacatataaa atagttgttg acatgttaga
     1141 aatgacatga taagataact gagttgttaa aaaagaacca cttgtgaagc ttgattctat
     1201 cgaaaaaaac ttcaaaaaag ttcttgacaa ataacaaatg ataagttatg ataaatgagt
     1261 tgctgtaaga tttcgaaaaa cgaaatcaaa aaaactttta aaaaagagtt gacaattact
     1321 tgtctctctg atatgatata aaagttgcta cggcagccaa aaacgaatcg gggaacactc
     1381 ctgatgaagt agacctttga aaactgaaca aagtaagaca aaccaaatgt gtagggcgtc
     1441 ttgattcaat tcaagacaac aaaca
//
LOCUS       DAZKOU010000195         1459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000195 DAZKOU010000000
VERSION     DAZKOU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.203"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..144)
                     /locus_tag="ACG827_002909"
                     /pseudo
     CDS             complement(1..144)
                     /locus_tag="ACG827_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(186..1312)
                     /locus_tag="ACG827_002910"
     CDS             complement(join(186..1084,1084..1312))
                     /locus_tag="ACG827_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0484978.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          427 a          260 c          238 g          534 t
ORIGIN      
        1 ctccagataa gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc
       61 ccaacgaatg atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta
      121 cagaagagtt cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga
      181 gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga
      241 atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga
      301 tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca
      361 tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt
      421 attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca
      481 gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga
      541 atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt
      601 aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg
      661 tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct
      721 tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt
      781 aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt
      841 aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt
      901 gaaatcttct ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta
      961 taaaaataat agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa
     1021 tccttacacc atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg
     1081 ctgcggcttt ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt
     1141 cagctacttc ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt
     1201 tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag
     1261 tttgattcag ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc
     1321 atcctctcgt agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga
     1381 tactaacacc aagttatctt tagaaccatt tcagatacat atgaaacaat tacatatgat
     1441 tttttcataa aaaaacagt
//
LOCUS       DAZKOU010000196         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000196 DAZKOU010000000
VERSION     DAZKOU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.204"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG827_002911"
     CDS             complement(150..1337)
                     /locus_tag="ACG827_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0484979.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKOU010000197         1362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000197 DAZKOU010000000
VERSION     DAZKOU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.205"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1362
                     /locus_tag="ACG827_002912"
     CDS             82..>1362
                     /locus_tag="ACG827_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0484980.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAZKOU010000198         1352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000198 DAZKOU010000000
VERSION     DAZKOU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.206"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG827_002913"
     CDS             <1..459
                     /locus_tag="ACG827_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484981.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG827_002914"
     CDS             473..904
                     /locus_tag="ACG827_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484982.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..1043
                     /locus_tag="ACG827_002915"
     CDS             906..1043
                     /locus_tag="ACG827_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0484983.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            1081..>1352
                     /locus_tag="ACG827_002916"
     CDS             1081..>1352
                     /locus_tag="ACG827_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484984.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          506 a          196 c          278 g          372 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga agaagcagat aaaatcatta
     1021 gcaaagaaga gagcgcatct taattgatgt gctttttatt ttgattcaag gagttgtcac
     1081 atgattaatt taggggaatg gggagcgata gcaggatcaa taaccgctat cgtttctttg
     1141 attttattag taataaaacc aattactgca tctttctcga agattactga gactctttca
     1201 aaagtaagtc acaatttaga tttgctgact aaagatttag aatcgagcaa atcagatcga
     1261 ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca tacagaaaaa
     1321 ttggtggaac acacgcaaca aattaaaact tt
//
LOCUS       DAZKOU010000199         1341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000199 DAZKOU010000000
VERSION     DAZKOU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.207"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..898
                     /gene="erm(B)"
                     /locus_tag="ACG827_002917"
     CDS             161..898
                     /gene="erm(B)"
                     /locus_tag="ACG827_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0484985.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1068..1328
                     /locus_tag="ACG827_002918"
                     /pseudo
     CDS             1068..1328
                     /locus_tag="ACG827_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          514 a          222 c          219 g          386 t
ORIGIN      
        1 aaacagacta aaaacagtga ttacgcagat aaataaatac gttagattaa ttcctaccag
       61 tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc gtttaacttc
      121 tgtatttgtt tatagatgta atcacttcag gagagattac atgaacaaaa atataaaata
      181 ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac aattgaattt
      241 aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa cgacgaaact
      301 ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat tcaacttatc
      361 gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata ttctacagtt
      421 tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc atttaagcac
      481 acaaattatt aaaaaagtgg tttttgaaag ccgtgcgtct gacatctatc tgattgttga
      541 agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc tcttgcacac
      601 tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa
      661 agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag ataaatattg
      721 gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac
      781 taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccattac
      841 ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt
      901 ctatgagtcg cttttttaaa tttggaaagt tacacgttac taaagggaat ggagataaat
      961 tattagatat actactgaca gcttccaaga agctaaagag gtccctagcg cctacgggga
     1021 atttgtatcg ataaggggta caaattccca ctaagcgctc gggacccctt gtaggaaaat
     1081 gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt ttatggttgt tcaaattatc
     1141 ctgaatgtaa gtttacttta gctgaacatt ttagaaagaa aaaactaacc aaaacgaatg
     1201 taaaagaatt actggaggga aaagaaaccc tggtaaaagg aatcaaaaac aaagagaaaa
     1261 agccctacaa tgccgttgta aaaattgggg aaaagggata tattgatttt atctcttttt
     1321 caaaataaac ataaaagccc t
//
LOCUS       DAZKOU010000200         1331 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000200 DAZKOU010000000
VERSION     DAZKOU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.208"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..208)
                     /locus_tag="ACG827_002919"
     CDS             complement(<1..208)
                     /locus_tag="ACG827_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484986.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
     gene            complement(305..757)
                     /locus_tag="ACG827_002920"
     CDS             complement(305..757)
                     /locus_tag="ACG827_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484987.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(764..>1331)
                     /locus_tag="ACG827_002921"
     CDS             complement(764..>1331)
                     /locus_tag="ACG827_002921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484988.1"
                     /translation="FYGGEIMKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVD
                     LEIKNKLVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIV
                     PRMEKVDWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQ
                     LGKAAAKELAKVNIVAGIAAAAASCALE"
BASE COUNT          478 a          262 c          130 g          461 t
ORIGIN      
        1 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
       61 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
      121 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
      181 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
      241 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
      301 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
      361 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
      421 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
      481 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
      541 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
      601 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
      661 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
      721 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
      781 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
      841 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
      901 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
      961 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
     1021 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
     1081 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
     1141 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
     1201 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
     1261 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
     1321 ctccataaaa a
//
LOCUS       DAZKOU010000201         1301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000201 DAZKOU010000000
VERSION     DAZKOU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.209"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACG827_002922"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACG827_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACG827_002923"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACG827_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          461 a          217 c          177 g          446 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt t
//
LOCUS       DAZKOU010000202         1294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000202 DAZKOU010000000
VERSION     DAZKOU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.210"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG827_002924"
     CDS             <1..1244
                     /locus_tag="ACG827_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0484989.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZKOU010000203         1284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000203 DAZKOU010000000
VERSION     DAZKOU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.211"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..483)
                     /locus_tag="ACG827_002925"
     CDS             complement(112..483)
                     /locus_tag="ACG827_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484990.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(485..886)
                     /locus_tag="ACG827_002926"
     CDS             complement(485..886)
                     /locus_tag="ACG827_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484991.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(900..1283)
                     /locus_tag="ACG827_002927"
     CDS             complement(900..1283)
                     /locus_tag="ACG827_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484992.1"
                     /translation="MSEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSE
                     IEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYA
                     VVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          409 a          231 c          170 g          474 t
ORIGIN      
        1 ggacgcacgt agcgatcatc ggcgttatga ctatataaag tatactttcg ttgatccaga
       61 aagatacaaa gattaaataa aaaaataaca aacaaactcg cttgaaattc attaggtgag
      121 tttgtttatt attttttctg cttctcgatt catcagcgcc atatctgttt tcgtaatggt
      181 ttcattagga atatagtcag cacattttct tttctctaac attctatacc cactattatg
      241 tattcctaag gtattcaatt ccttaactgt tgaaaacaaa ttccaaacgg tttggtggct
      301 tccttttccg ctacttccat tataaataat atcataattt tctttcaaat actcatcaga
      361 ttttttaaaa gcactgtagt atgctcttga aacagcagtt cttctataag attctttaga
      421 attatattta tcatctgatt caatttgttt ggaaatagat aaatattcat taaagtcaaa
      481 cattttattc ccccaaagtt gctactacag atttccccat atatttatag tcagataata
      541 cattatcgag agtatccaaa acatcgtaat ctgaggaaaa caattcagat gttgttttga
      601 taacaaagaa atacaagttt aaatcttcat catattttat attgatttca gcttttcttt
      661 ttaaaaccgt atttatcaca tcaaaaattt tgccatcatt acttgaaatt gtgttaatct
      721 cattttttgt agtaattaca agttgattat catttttagt tttcggagta gaacttgcat
      781 ccacaacaac tggagatgct agaattaccg atgcgcaagc aaaaatcgca gcaaaattta
      841 ttaagctacc actccgatgt gtatttgatg gtactatatc aagcatccgt atcgtctcct
      901 taattttttt ccactggttt gttgaccttg atatcaaaga tttgccttcc aatctcagtt
      961 ccattaaatt taacaacagc ataatgctct cctgcttctt caaccaagac atttctggcg
     1021 tttatattaa agtttatatc gttaacttga ggaggcaagc ttaccgatac ttcacccgta
     1081 gaaaaaattt gtttttcatt cttgtttaaa acttcaattt ctactatttt tatcttttct
     1141 gtatcctcta tctcagacac cacagctgtt acagaaaatg ataaagcagt aggaattaaa
     1201 ggcgcattaa tagaaagagc tggattaatt actttattaa taacattagt gccaaattgc
     1261 tgactttcta catcttctga aatt
//
LOCUS       DAZKOU010000204         1281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000204 DAZKOU010000000
VERSION     DAZKOU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.212"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(283..1143)
                     /locus_tag="ACG827_002928"
     CDS             complement(283..1143)
                     /locus_tag="ACG827_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0484993.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT          395 a          238 c          183 g          465 t
ORIGIN      
        1 ttttaatata aaattttata ttgtaaaaaa acaaataagg gaaacaaaca acacattttt
       61 gtgtgttgct tatttccctc taactatcgt tttctatttg atggttaacc tacttaaaat
      121 aaatactgca acgacaaacg gagatcaagc atgctttgta acgtatttta cgataattac
      181 gactttattt tattactcta tcttgctgcc atacccgtct atccctaatc atctttggtg
      241 aattttttca aagttagcat aacaaaaata agtatttttt ccttagttaa tcaatagtat
      301 catttttttc atatgattta attcatcaat taattttttg tttttattaa ttttagcaaa
      361 agctagtgca tcttgaatca attcgagcac ttctgccttc gacttatttt tagcctgtgc
      421 gttcttcgct aattgggcag cagctcgtgc taagtaataa gtcaaatggt tatccgaaca
      481 aatatcgatt gcgtaattta gtagtgcatc acttgtttcc aaatcaccgt cttgcgcaaa
      541 aaaaacacca caatgaaaga cgatatttaa cacccgccag atatcttctg ttgtacgaat
      601 cggatacgta taaatttttt caaacactcg attgaaataa acttctgctt tttccaactc
      661 tttttctcta gcatagacca ttccaatacc tgtatatgcc aataaacgaa acaacgtgtt
      721 ggctgtttct gccatcaaca actgatcaaa atggaacaac acatccgtaa ttggtgcatt
      781 cgtaaaaatc atttcaaatc cttttagata gtagtaacgt gcaatcagct cagactcgtc
      841 aattaccttg ggatcaatgg ctgctaaaat ctgtgtcaat tgttcatatt cactcgtaat
      901 cagtgaaaac tctgcttttt ctagctgttc atcaagttgt gtattgatca cgttcatcga
      961 agaaaaaagt tcaactaacg gaagattgat tcgattacat aatttgatta aaatttttaa
     1021 cgaagggact tgaccattgt tctcaaattt acttaatgtt gcttgtgtac aaattccttc
     1081 agacacttct ttttgagata agttggcagc ctttcttgcc gtaataaatt tttcaatgtt
     1141 cattattttc tctccaatca gacctcacac gaaaatcatt tctgtctcga ttttagcatg
     1201 ttttctttgg gaatgcttta ggttctgttg caaagtttta aataaagaat aaagtccctt
     1261 acggtatcta tgatttaagc t
//
LOCUS       DAZKOU010000205         1246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000205 DAZKOU010000000
VERSION     DAZKOU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.214"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG827_002929"
     CDS             200..742
                     /locus_tag="ACG827_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0484994.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACG827_002930"
     CDS             898..>1246
                     /locus_tag="ACG827_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0484995.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAZKOU010000206         1215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000206 DAZKOU010000000
VERSION     DAZKOU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.215"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG827_002931"
     CDS             414..947
                     /locus_tag="ACG827_002931"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0484996.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAZKOU010000207         1214 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000207 DAZKOU010000000
VERSION     DAZKOU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.216"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1026
                     /locus_tag="ACG827_002932"
     CDS             <1..1026
                     /locus_tag="ACG827_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0484997.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
     gene            complement(1080..>1214)
                     /locus_tag="ACG827_002933"
     CDS             complement(1080..>1214)
                     /locus_tag="ACG827_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0484998.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          207 g          401 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaa
//
LOCUS       DAZKOU010000208         1193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000208 DAZKOU010000000
VERSION     DAZKOU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.217"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..1157)
                     /locus_tag="ACG827_002934"
     CDS             complement(3..1157)
                     /locus_tag="ACG827_002934"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0484999.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT          331 a          258 c          220 g          384 t
ORIGIN      
        1 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
       61 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
      121 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
      181 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
      241 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
      301 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
      361 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
      421 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
      481 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
      541 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
      601 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
      661 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
      721 gccaagctgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
      781 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
      841 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
      901 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
      961 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     1021 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     1081 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     1141 tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta ctt
//
LOCUS       DAZKOU010000209         1148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000209 DAZKOU010000000
VERSION     DAZKOU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.218"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1083
                     /locus_tag="ACG827_002935"
     CDS             <1..1083
                     /locus_tag="ACG827_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0485000.1"
                     /translation="TQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTK
                     ELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFK
                     SMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLVMDMNAS
                     YCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYW
                     KLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKR
                     DTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNNKIKLIK
                     RIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          356 a          248 c          222 g          322 t
ORIGIN      
        1 actcagatgt tgaaagtcaa agaaaagttg actattttag aattaaaacg tacccgtttt
       61 ctctgccatg attgtggacg gaccttttct gcgaaaacgg acttagttga tgaacatcat
      121 caactaacaa aggaactgaa gcaggcgatt ctcatggaac tgtacgaaaa tcaatcacgt
      181 aaactaatcg ctaagaagta cttcgtctcg gatgggacgg tgacacggat cttgcgtgaa
      241 gcaacgaagc attaccagcc acgtatgaac ttccttccga cggtgttgtg tatggatgag
      301 ttcaaatcaa tgaagtctgt ttctggttcc atgagtttta tttgtgttga tgggacaacc
      361 aatcagctat ttacgatcct tgaagatcgc agactttaca aactaactca atacttcctg
      421 cgctttccta gaaaggcccg tttaaaggtc aagtacctag tgatggacat gaatgccagc
      481 tattgtcagt tgcttaaaac agtctttcca aatgcggaga tcgtaactga ccgctttcat
      541 attgtccaac acatcaatcg cagcttcaat caattacgtg tccagatcat gaatcgtttt
      601 cgaacctcac actctgaaaa tcagaagaag taccgtcggc tcaagcgata ttggaaactc
      661 ttactcaaag attccacgac attggagcca ctcaaacggc attatcatcg actgtttaaa
      721 cgaccaatta gtcaaacaga aatcgttgat gagctgttaa gctacaacga agaattgcga
      781 actgcgtatc atttttgtca attattacgc tattatttcg ttaaacgaga tacggaaggc
      841 tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa aaagaaattc
      901 acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgcc ccactcaaac
      961 ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc gtttggttat
     1021 cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat ctttgaaaac
     1081 taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta ttcaaatttg
     1141 tcttcgcc
//
LOCUS       DAZKOU010000210         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000210 DAZKOU010000000
VERSION     DAZKOU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.219"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG827_002936"
     rRNA            <1..>1073
                     /locus_tag="ACG827_002936"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZKOU010000211         1069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000211 DAZKOU010000000
VERSION     DAZKOU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.220"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG827_002937"
     CDS             <1..969
                     /locus_tag="ACG827_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0485001.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAZKOU010000212         1062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000212 DAZKOU010000000
VERSION     DAZKOU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.221"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG827_002938"
     CDS             <1..963
                     /locus_tag="ACG827_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0485002.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          366 a          195 c          212 g          289 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc ca
//
LOCUS       DAZKOU010000213         1055 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000213 DAZKOU010000000
VERSION     DAZKOU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.222"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..793
                     /locus_tag="ACG827_002939"
     CDS             101..793
                     /locus_tag="ACG827_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0485003.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          458 a          153 c          175 g          269 t
ORIGIN      
        1 tttatattaa aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta
       61 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      121 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      181 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      241 gcttgatcat acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc
      301 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      361 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      421 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      481 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      541 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      601 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      661 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      721 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      781 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
      841 tatactaagt agttcagttt tggagtacaa aatgacaaaa tgtacttcaa aatggactac
      901 ttttttcatt ttaacttaca taccaaatta catacaaaat gatttataaa atgcaattga
      961 aaatgataat tgaatgaaaa tttgtgattt tgtggtatta tgataatata acttatagaa
     1021 aaaattgctt attcaactaa cgggcaggat aatga
//
LOCUS       DAZKOU010000214         1053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000214 DAZKOU010000000
VERSION     DAZKOU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.223"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..902
                     /locus_tag="ACG827_002940"
     CDS             87..902
                     /locus_tag="ACG827_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0485004.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
BASE COUNT          396 a          158 c          178 g          321 t
ORIGIN      
        1 attacatagg ataatatatt aaggtgctgg actacaagta aaaaatactg tatgatcatg
       61 acagattcaa gaaaggagca ataagattgg ttaagatagc aggactttct aaaggtggaa
      121 aaaccgtttc acaactttat aaacaaaact acagagactg gagacagttc agaaaaaaca
      181 ttaaggattc ttactttatt ctaccaacag aacttgaaaa atatttaccg aataacaaag
      241 ctattaattt atatctgtat tactgtttta aggcaaagaa tgaaggcggg gattcctggc
      301 attcggtcaa taccatagca gagtctctag gagtgacaac taaaactata aacacctgga
      361 ataaaacctt agttgattta ggtatgattg cacgtataga cgataaccat ctcagtaaat
      421 cgacaatttt acttcctctc tctagttact ataaattagc ccttgatagt tctttaaagg
      481 atgttaccga gagcactgtc catgaaattg atggcaactt ggttgcagtt tatcatttgt
      541 ttgaatggag aaaaaatgag gctgatgaaa attataacgt tccttataac acactctgtt
      601 gtgtttttaa aagaaaaaca caaaaagata ctatctataa atttatcttg gtccaagatg
      661 agaaaataag taatttcgaa ttaagcactc cttctagtaa gatttatgac gatgcatata
      721 gatttgaatg tcaagatcta gaagccctcc tttccgcaaa taatctaaac aatatagaac
      781 atgaaaactt tgttgtaaac actagattta atttagtttc tgaaaaggac tcagatttgt
      841 tagatttgct gatagagctt actaaaaatc tagataagaa agaaaacatt actgtactat
      901 aactagcatc ggtccatcat taagtttgga ctattagaac acttgctatt acagaaaaag
      961 gtatgctagc cacgcttact atttattggt atcaaacaag tatcttaatt tgaaaggaag
     1021 atgatttatg gctaatttat atatagagaa caa
//
LOCUS       DAZKOU010000215         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000215 DAZKOU010000000
VERSION     DAZKOU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.224"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1003
                     /locus_tag="ACG827_002941"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACG827_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAZKOU010000216          991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000216 DAZKOU010000000
VERSION     DAZKOU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.225"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..94)
                     /locus_tag="ACG827_002942"
     tRNA            complement(21..94)
                     /locus_tag="ACG827_002942"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="ACG827_002943"
     tRNA            complement(108..181)
                     /locus_tag="ACG827_002943"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="ACG827_002944"
     tRNA            complement(209..281)
                     /locus_tag="ACG827_002944"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="ACG827_002945"
     tRNA            complement(301..374)
                     /locus_tag="ACG827_002945"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="ACG827_002946"
     tRNA            complement(384..457)
                     /locus_tag="ACG827_002946"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="ACG827_002947"
     tRNA            complement(468..553)
                     /locus_tag="ACG827_002947"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="ACG827_002948"
     tRNA            complement(570..641)
                     /locus_tag="ACG827_002948"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="ACG827_002949"
     tRNA            complement(653..725)
                     /locus_tag="ACG827_002949"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="ACG827_002950"
     tRNA            complement(743..824)
                     /locus_tag="ACG827_002950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="ACG827_002951"
     tRNA            complement(832..904)
                     /locus_tag="ACG827_002951"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="ACG827_002952"
     tRNA            complement(908..980)
                     /locus_tag="ACG827_002952"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DAZKOU010000217          989 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000217 DAZKOU010000000
VERSION     DAZKOU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.226"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACG827_002953"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACG827_002953"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0485005.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          347 a          128 c          176 g          338 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttg
//
LOCUS       DAZKOU010000218          957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000218 DAZKOU010000000
VERSION     DAZKOU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.228"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG827_002954"
     CDS             <1..878
                     /locus_tag="ACG827_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0485006.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          300 a          212 c          191 g          254 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcggctctat actttttgat gttggtgaac ttcaccaaca tcttttt
//
LOCUS       DAZKOU010000219          955 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000219 DAZKOU010000000
VERSION     DAZKOU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.229"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..141)
                     /locus_tag="ACG827_002955"
     tRNA            complement(68..141)
                     /locus_tag="ACG827_002955"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(105..107),aa:Ile,seq:cat)
     gene            complement(696..>955)
                     /locus_tag="ACG827_002956"
     CDS             complement(696..>955)
                     /locus_tag="ACG827_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485007.1"
                     /translation="WFFDKSRNAGIGFRTKRSTSSEKKWVYSQTIFYGGVISISLLSS
                     TLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSKNN"
BASE COUNT          348 a          154 c          151 g          302 t
ORIGIN      
        1 aaagctttag cttgtataat actcttcaaa aaattctaac acagaacgat tcaattggct
       61 acattaatgt accctgtagg actcgaacct acgaccggac ggttatgagc cgtctgctct
      121 gaccaactga gctaagggta gtggttgttg ccacataaag ccataaacaa tcaaccagta
      181 gaatgcgtgg caacaaacct gttatcgcat atcttggagt gtgactattt atgagtgata
      241 gtgaagatat gcgtctccat atatgtatgg ttgagacaga taaacatgta tgaaaaacgt
      301 tgttaaatca acgttttttt attttggtca aggagagaaa agtagagaaa tacaaaattt
      361 tttgcattga ttttgcattc aaaaaaacat tcaaacattt agaaatcccc aatacgtatt
      421 caggaaaatc tattttaagg tttacaaaaa gaacttttgt tcgtacactc tttttgaggt
      481 gaacttttat gctgatggaa ggaaaaacac aattatggtt taagcatgtt ccttcaaata
      541 agttttttaa ttataaactc tagagaaatt aattcatcct aacataaaat aattgttcac
      601 tcaaaagttt ttctagaaca attaaaacag caggcttcac attcgctata catttgtaag
      661 aagtaataat tcctgtacta aaaggcataa ttgctttaat tattttttga cttctcttca
      721 aacacaagaa gtagttgtgt aataattgca gaaataagga ttccaataat agatataaaa
      781 tttgacatgg aaacgtctat aacattaaaa gaataaagtg ttgaagaaag taaactgatt
      841 gaaataaccc ctccgtaaaa aatagtctgt gaatataccc actttttttc agaagacgta
      901 cttcgtttgg ttctaaaacc tatccctgca tttctactct tatcaaaaaa ccaaa
//
LOCUS       DAZKOU010000220          910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000220 DAZKOU010000000
VERSION     DAZKOU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.230"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG827_002957"
     CDS             <1..355
                     /locus_tag="ACG827_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485008.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACG827_002958"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACG827_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DAZKOU010000221          895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000221 DAZKOU010000000
VERSION     DAZKOU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.231"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            252..875
                     /locus_tag="ACG827_002959"
     CDS             252..875
                     /locus_tag="ACG827_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0485009.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          330 a          129 c          167 g          269 t
ORIGIN      
        1 aactaatgtc cttatttttg tgttcacatt tctgttacct aagaaatact taaaggaggt
       61 gatgtagcca gcaacgacta caacttgaac ccgctatcta cagtttattt gttgtttctt
      121 ttgggtatac tctaatggta caaatccata tttgcttaaa gcgttgattt ttcagcggtt
      181 tttctttatt gcaaagtgta ccctaagacc tgtatactaa tagtaaatag attggatagg
      241 aatgatggca aatgtattta gcttatttaa gagtttcttc cgacgatcaa agtttagctc
      301 ggcaacataa gctaattgaa aattggtcag tcaaaaataa tattgctgaa gacgatttaa
      361 aaattttcga ggaaaaagtc tctggaaaaa atattgaaaa cagaccagag ctgcaagagc
      421 ttatttcttt catacgtgaa cgagatacga ttattattcc tagtcttgat cgattagcaa
      481 gaaatagtaa agatacaaaa gacttacttc aattgtttcg tagtaaaggt gcttctattg
      541 aaatattaga cctaccgtcg tttaatgggg taactgaccc tgcattaaga gacttgctaa
      601 caaatttagt catagaagtg tttagctatg ttgctgaaaa cgaacggaaa aaaatcaaag
      661 agcgtcaaaa acaaggcatt gaaattgcaa agaaaaatgg tcgttatact ggtaaaaaga
      721 ttcaatacca tgccaatgca acaggaaaaa acaaattgat ttacgatgaa attgttgatg
      781 gtctgaagaa aaaagaaact atttctaaaa tagcaaagaa cgcaggagtt acaagaaaaa
      841 ccgtttatcg agtaaaaaga gatttaggaa tgtaatatgt tccagttaga aagaa
//
LOCUS       DAZKOU010000222          873 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000222 DAZKOU010000000
VERSION     DAZKOU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.232"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>873
                     /locus_tag="ACG827_002960"
     CDS             <1..>873
                     /locus_tag="ACG827_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0485010.1"
                     /translation="KSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQ"
BASE COUNT          263 a          174 c          176 g          260 t
ORIGIN      
        1 gtaagtcttg ccaaagaaca ttcgtagcgg atacctctgt tgctgaaaaa cactgtttca
       61 ttagtcaaaa agttcgttgg tctgtcattg ctcgcttaaa agagaatact tccatgacgg
      121 aaatcgctcg tcagaaaaat atttccactt cttcggtcta tcgtgtcatg aagcgattct
      181 accgcccatt gaatcctttc aaacagactt tgccaaaggt cttatgcttt gatgaattca
      241 aatcggttcg aagtgttgcc agtgcaatga gttttatcat gatggacgga caaagccatg
      301 cattattgga tattgtcgaa aatcgacgct tgccttattt agaacgctat ttttctcggt
      361 tttcattagc gactcgtgaa gccgttcaat tgattgttat tgatatgtat gcgccttatg
      421 tttcgttggt gaaaaagctt ttcccgaatg ctcagttgat catcgatcgt ttccacatcg
      481 tccaacatat tggcagaact ttcttgaatc atcgagtgaa agaaaccacg gtacgactta
      541 aaggaaattc ccactctcaa cgagggttag ggaaaaaatt gaagcgctat tggaaactcc
      601 tacaaaaaga agaagcaaag ttaaactatg aaaaacgggt ttggcgtgcc agttttaaag
      661 attatttgac tgaaagtgag attgccgatc gactgttagc tgcttgtccc aacttacgcc
      721 aaggctatca gttataccaa gacattcttt atgcggtaaa gaaaagagat cagtcactat
      781 ttgaggattg tttaactaga gacattagcg gtctgccaga aacctataca accactttac
      841 gaacgtttaa aaagtttctg ccacagatcc aaa
//
LOCUS       DAZKOU010000223          868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000223 DAZKOU010000000
VERSION     DAZKOU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.233"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..>868
                     /locus_tag="ACG827_002961"
     CDS             2..>868
                     /locus_tag="ACG827_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485011.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLI"
BASE COUNT          272 a          115 c          129 g          352 t
ORIGIN      
        1 catgaggtgg tatatgaatt tgaaaaaaat tttttctgta aaaatattta tagtttatgc
       61 atttgttagt ttattatatt tatttccagc atttaaaaac atggaaatat ttggctttta
      121 tgatacggca tttcacatta atcgtgcgtt aagtttagaa tctattttta gctcccctat
      181 taattttgaa acgtttagat catatggcat gcaagtaaat aatttttacc catggttaac
      241 tctatatcct ttatttttat tgatcaagtt tactaattta gcaataggct ataacctttt
      301 tctttatatt gttacgctca ttactctatt tatatgtcat tatgtaatgt atgaaattac
      361 caaaaaacat gttactagct cctttttcgc tattatatat acaacatctt cttttaggag
      421 tgtagaaata tttcttcgag gggcaatggg ggaattactc gcaatgtcaa tacttcctct
      481 tattttactc gggtttataa agctttacga ctcaaaaaaa gaatcttggg ttatgttggc
      541 gattagcatg actttattaa tttacactca tgttctttct gtagctatga ctatggtaat
      601 gattataatt gctttaatta ttcaatttta tcggaaaaag aaaatagaaa tcttcttaat
      661 tattaaatta ataaaagctg ctatagttac tttacttctt tcactagcgt ttataggtcc
      721 tatgattgag cagacattgt atcaagatct gaatagacct tatgtggata tattacaaaa
      781 aagagcaatt tatctgggta aagagttttt gattggaagt atagattcag aattactatc
      841 atttggtatt ggcttaactt tgcttatt
//
LOCUS       DAZKOU010000224          838 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000224 DAZKOU010000000
VERSION     DAZKOU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.234"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..753)
                     /locus_tag="ACG827_002962"
     CDS             complement(40..753)
                     /locus_tag="ACG827_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0485012.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
BASE COUNT          269 a          172 c          107 g          290 t
ORIGIN      
        1 tataaaaaaa taagctctga aaaaataatt ttcccgagct tatttttcaa caaaaacagc
       61 ttcaaaaaca aagtcttgat ataaatgtct cgataccgaa tactcgaaag gtttaccaga
      121 cgatagagca acaactttgt ctatctcaat cataaattca cctggatcta tttttaatat
      181 ttttgcgtca aattcattcg cagtatctcc tttgatacgt atgtgcgaag tctttatttc
      241 cagacccagt tcatttgtga tataagcata tacagatttc tttaaatgtt ttggttccaa
      301 gcctggtatg actgctaatg gcatgaatgt ttgctcaatc gagtatggtt gacaatcaat
      361 aaaacggctt cgtacaatat tatatacata gtcttttaca ctgatactta atttatcagc
      421 tacttttttc gatggaagtt caattgagaa agtatggatt tctgaatcta cgtgttcttc
      481 accaaccacg ctaaacaaac cttcgtttgg accaagaact cgtgagttat caattggtgt
      541 tctgacgact tcagtcccat acccacttct acttcgcaaa actccttcgg aaacaagtaa
      601 gtctacgcct ttctttactg ttaacttgct tgtatgataa ataactgcca attcatctcc
      661 tgtaggaagt ttctcaccaa ttttatactc tccatttaca atttttttct ttatatctaa
      721 gtaaatttcc atatacttcg caattttttt caatggtgac cctccaatca aaaacatatc
      781 ttcatctata ctttaacata aataaaccga taaaatccac atcaaaatta tacacaca
//
LOCUS       DAZKOU010000225          836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000225 DAZKOU010000000
VERSION     DAZKOU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.235"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACG827_002963"
     CDS             136..309
                     /locus_tag="ACG827_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485013.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          280 a          139 c          104 g          313 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcata
//
LOCUS       DAZKOU010000226          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000226 DAZKOU010000000
VERSION     DAZKOU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.236"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG827_002964"
     CDS             <1..672
                     /locus_tag="ACG827_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0485014.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZKOU010000227          793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000227 DAZKOU010000000
VERSION     DAZKOU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.237"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG827_002965"
     CDS             85..624
                     /locus_tag="ACG827_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485015.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          309 a           97 c          146 g          241 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aat
//
LOCUS       DAZKOU010000228          791 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000228 DAZKOU010000000
VERSION     DAZKOU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.238"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>791
                     /locus_tag="ACG827_002966"
     CDS             <1..>791
                     /locus_tag="ACG827_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0485016.1"
                     /translation="AHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLV
                     LPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRK
                     PNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKV
                     IITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFA
                     DPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRN"
BASE COUNT          269 a          137 c          153 g          232 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      781 aaacgaaata a
//
LOCUS       DAZKOU010000229          786 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000229 DAZKOU010000000
VERSION     DAZKOU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.239"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            358..>786
                     /locus_tag="ACG827_002967"
     CDS             358..>786
                     /locus_tag="ACG827_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0485017.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLD"
BASE COUNT          264 a          148 c          126 g          248 t
ORIGIN      
        1 accgaatgca aaacagaagt tctataagtt ataatcatag ttgttaggtg attcatctag
       61 taaaaacacc agatttcaaa tttcacacaa tttaaactag atcaatcatc aaccttcttc
      121 aaattgttag aaccctttcc cgaggagctg cagcataagc aaaaaaacaa acaggaagcc
      181 aaatggctaa gacatttttt ctattagtct agttgcttct cgtcgcaagt ttaagacttt
      241 agaaagttat ttctaatgat gtttgggttg ttttgatcaa tgcttatgca cagctcctta
      301 cctattattt atctcaacag atctaacaaa ttaatggact atttaaggag tttttttatg
      361 cttacgatct attcttctcg cacacatagt taccatctga tggcaaactg gctgaaagac
      421 catcaaatgc cttacagaga acgaattttc acacctaaac agcctttaac aaggaaagag
      481 atattttatt tattgtcatt aacagaaaac ggatttgacg atctattagc taaacgctcg
      541 attgattata aaaatttgaa agaccaaatc gaccaatgta cgacctcaga gcttgtcgat
      601 ctgatcgtct cgcattttac tttgttacaa cgtcccatca ttactgacgg aaagaaatta
      661 ttagtcggat tcaatgaaga tagcatgcgt ctttttatcc ctgtacaatg gcgcagaagt
      721 caactgaaat ctgtctataa cgattttaca tgggtaagca atctagaaaa ttctacggat
      781 ttagat
//
LOCUS       DAZKOU010000230          768 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000230 DAZKOU010000000
VERSION     DAZKOU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.240"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..528)
                     /locus_tag="ACG827_002968"
     CDS             complement(16..528)
                     /locus_tag="ACG827_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485018.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            complement(535..>768)
                     /locus_tag="ACG827_002969"
     CDS             complement(535..>768)
                     /locus_tag="ACG827_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0485019.1"
                     /translation="FNSSTEFKLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGT
                     YIKFLENYNDLKSTNNYQFGNLSYEMISKVLSL"
BASE COUNT          263 a          111 c           73 g          321 t
ORIGIN      
        1 ttaaaactaa catccttaat atcttaattt atccatattt tgagaaataa tacttttcca
       61 ttcagaaact aatttagtaa ggaatcttgc aatgttttgt ctcattaatt cattatttct
      121 attatctata agatctgtta gatatttttc aaattctaaa aagctatccg aattttcttc
      181 gacgattaaa tacttaagag aatttatatc ggaaaagata gattcaagtt tactaatctc
      241 attttctaaa tctatatttt cacgtgtgat tatctcatca ctttttctta aaaagctaaa
      301 aattgcatca gttgctttac acttttccat taaagaagta tattcttcaa tttttcttct
      361 ttctttatgg tcatttttta aatcagtttc aatgtctttt ttcatttgtt ctatactgag
      421 tttgttcttt ttgttctttt gagtaaatct aaaattcaaa cacgaaaaaa gtatgttagc
      481 aataacacct ataatagcaa ataaatcatt tatactattt aatttcattt atcatcacaa
      541 acttagaact ttacttatca tctcataaga caagtttcca aattgataat tattggtaga
      601 ttttaaatca ttataatttt cgagaaattt tatataagta cctctcatcg gtttatggct
      661 attaaaagtg ttaaaatcac tatcttcaac tatttcttga aacctattta atttttcaat
      721 aaaaaattgt gcataaagtg ctaacttaaa ttcggtagag ctattaaa
//
LOCUS       DAZKOU010000231          767 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000231 DAZKOU010000000
VERSION     DAZKOU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.241"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..230)
                     /locus_tag="ACG827_002970"
     CDS             complement(<1..230)
                     /locus_tag="ACG827_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFQ0485020.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAM"
     gene            complement(223..>767)
                     /locus_tag="ACG827_002971"
     CDS             complement(223..>767)
                     /locus_tag="ACG827_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0485021.1"
                     /translation="QKHDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLY
                     HGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIG
                     GPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDY
                     SDKLLNRIDKEILKVGNIDE"
BASE COUNT          265 a          123 c           90 g          289 t
ORIGIN      
        1 atcattgctc caatcagtag ccaagaaaaa cgcattttaa aaaaattttt ctttataaaa
       61 acctcataca aaactataat tccaccccaa gcaaaagcta ttttcatcgg gatattagtt
      121 acattaaaag tactaggcat aaaataaagc agacaaatga ttaaatttat tactcgaaac
      181 gcatctcgac ttaataaact cttctctaaa acaggtacta ttctattcat ctatatttcc
      241 aactttcaaa atttctttat caattctatt taatagttta tcagagtaat ccgatttaac
      301 ttttcttaca aataattttt cactttcttt tatttgatct atatcccttt catcaaacca
      361 tttgttcatg gaaggatcaa taatatgaat atttgctaat ttcgaagttt gtaaagtagt
      421 ttcttcaata tttttcaatt caagtggtaa atctggtcct ccaataacat ttttattttt
      481 gaacgaggaa ttaaaaaaaa gtgtatgaaa aaatttttca tctggggcaa acatacctat
      541 tttaaaattc aggtagtctt gttgattttg ttcatacatt tgtattaatt ctgttaggca
      601 ttctttcgac aaagcccacc attgagaacc atgatacaaa tcaaatttat ggtgtctaat
      661 ttttggggga agtttaatca tcctcagaca acgattaatt atagctctaa aaattttaaa
      721 tgcagttgtg ttattcctat taattctagg ataatcatgt ttttgat
//
LOCUS       DAZKOU010000232          761 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000232 DAZKOU010000000
VERSION     DAZKOU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.242"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            506..697
                     /locus_tag="ACG827_002972"
                     /pseudo
     CDS             506..697
                     /locus_tag="ACG827_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          296 a           91 c          123 g          251 t
ORIGIN      
        1 ggcagtggta tttcttcagt aagattaaaa gagaaagaat atactcaaaa gattattcat
       61 attcctaagc ctgaaattaa gcgttttgag ataaaagact attctgcttt agggctgttc
      121 tcaattggtt actggtgata aaaaataaga atactaaaac tatacgtaga atataagtaa
      181 tcggcatcaa tcctaattat gtgattgaat gccgattttt gttacgcgct atttttaatt
      241 atctgtaaga agaataattt tattttactc atattcgttt tcactttgaa gaagtaaatt
      301 gcaatttctt ataagttaag cagctatgca aaaggctatt tattctattg tgaaatataa
      361 gatgtagttg aaacaggata tatagaatca aaatacgaat cttattttga tttttctagt
      421 tttttgaaaa aagcaccctt tgatcgttgt attagtggaa aagagaaaaa ggagcatact
      481 tatcatgtca caaaactaca actaaatatc taaacaacac caatttgatg cctattgaaa
      541 aaaagtattg cgaaatgaag ccaagtctat tagaaaatga aataatagaa taaaagaaaa
      601 tgggaaaccg ctgaatgatg taaatgaagg taagtatcca cgaactaatt ttgatgaaca
      661 agatatctat tttttatttg tattggagat actagtttaa tatcaaaagt tatcagcagt
      721 cataattagt tatccaatgt aaaaagtaca aacaatagtt g
//
LOCUS       DAZKOU010000233          753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000233 DAZKOU010000000
VERSION     DAZKOU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.243"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..95)
                     /locus_tag="ACG827_002973"
     tRNA            complement(23..95)
                     /locus_tag="ACG827_002973"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60..62),aa:His,seq:gtg)
     gene            complement(98..171)
                     /locus_tag="ACG827_002974"
     tRNA            complement(98..171)
                     /locus_tag="ACG827_002974"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Trp,seq:cca)
     gene            complement(178..258)
                     /locus_tag="ACG827_002975"
     tRNA            complement(178..258)
                     /locus_tag="ACG827_002975"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(222..224),aa:Tyr,seq:gta)
     gene            complement(260..335)
                     /locus_tag="ACG827_002976"
     tRNA            complement(260..335)
                     /locus_tag="ACG827_002976"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Phe,seq:gaa)
     gene            complement(371..443)
                     /locus_tag="ACG827_002977"
     tRNA            complement(371..443)
                     /locus_tag="ACG827_002977"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(408..410),aa:Asp,seq:gtc)
     gene            complement(453..525)
                     /locus_tag="ACG827_002978"
     tRNA            complement(453..525)
                     /locus_tag="ACG827_002978"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(490..492),aa:Val,seq:tac)
     gene            complement(541..612)
                     /locus_tag="ACG827_002979"
     tRNA            complement(541..612)
                     /locus_tag="ACG827_002979"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(577..579),aa:Glu,seq:ttc)
     gene            complement(627..716)
                     /locus_tag="ACG827_002980"
     tRNA            complement(627..716)
                     /locus_tag="ACG827_002980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(680..682),aa:Ser,seq:gga)
BASE COUNT          209 a          206 c          174 g          164 t
ORIGIN      
        1 gctatacccc aagaataaaa agggcgaatg atgggaatcg aacccacgaa tgccggagcc
       61 acaatccggt gcgttaacca cttcgccaca ttcgccatgg caggggcagc aggaattgaa
      121 cccacactaa cggttttgga gaccgctgtt ctacctttaa actatgcccc tataaaatgg
      181 aggagagtgg attcgaacca ctgaacccta aggaacggat ttacagtccg tcgcgtttag
      241 ccacttcgct actcctccat ggtggcgcgg gacagaatcg aactgccgac acacggagct
      301 tcaatccgtt gctctaccaa ctgagctacc gcgccatatt ttaaaattat taattgaata
      361 acaagaacga cggttccgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcat
      421 gttaacccct acaccacgga accagttcat tatggaggtt aacgggatcg aaccgctgac
      481 cctctgcttg taaggcagat gctctcccag ctgagctaaa cctccaacat tcaattaaaa
      541 tgacccgtac gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt
      601 gaccaacggg ccaggtaaat ttgctacgga gagtaaggga ttcgaaccct tgagacagtt
      661 tggcaccatc tacatgattt ccaatcatgc tccttcggcc tctcggacaa ctctccagag
      721 tgaaagaagc tcaatactct ttctcaaaat gag
//
LOCUS       DAZKOU010000234          746 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000234 DAZKOU010000000
VERSION     DAZKOU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.244"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..>746)
                     /locus_tag="ACG827_002981"
     CDS             complement(101..>746)
                     /locus_tag="ACG827_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFQ0485022.1"
                     /translation="MKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAI
                     VTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLF
                     HTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETL
                     AQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          204 a          171 c          143 g          228 t
ORIGIN      
        1 ttataaggtg ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc
       61 ctcggatctg attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg
      121 tatcagatcc gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga
      181 tcggtgtttc atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat
      241 caaacaattc tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta
      301 ctttgacaga tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac
      361 tcaatgaacg agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt
      421 cctttccccg gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa
      481 aggcttcttt taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc
      541 gattaaacaa atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag
      601 taagatccgt aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat
      661 tcgctgtttt cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg
      721 tagatgtcaa tccgcggcgt ttcatg
//
LOCUS       DAZKOU010000235          731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000235 DAZKOU010000000
VERSION     DAZKOU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.245"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(333..446)
                     /locus_tag="ACG827_002982"
                     /pseudo
     CDS             complement(333..446)
                     /locus_tag="ACG827_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          268 a          115 c          112 g          236 t
ORIGIN      
        1 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
       61 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
      121 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
      181 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
      241 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      301 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      361 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      421 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      481 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      541 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      601 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      661 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      721 atataaaaaa a
//
LOCUS       DAZKOU010000236          719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000236 DAZKOU010000000
VERSION     DAZKOU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.246"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..>719)
                     /locus_tag="ACG827_002983"
     CDS             complement(334..>719)
                     /locus_tag="ACG827_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HFQ0485023.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT          247 a          101 c          125 g          246 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgat
//
LOCUS       DAZKOU010000237          712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000237 DAZKOU010000000
VERSION     DAZKOU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.247"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..479)
                     /locus_tag="ACG827_002984"
     CDS             complement(<1..479)
                     /locus_tag="ACG827_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HFQ0485024.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     "
BASE COUNT          219 a          133 c          109 g          251 t
ORIGIN      
        1 gcaagcaatc ctagctcttc tttaatattc tcctggatca tttgagccag cttctttctc
       61 cgttgacttc ttgttccttc agtcgtaaaa agattcattg atctggtcac ttttaagatc
      121 gattcatcga tcgagtaaat caataaatct tcgtctgcta cataccttct gaaaatattg
      181 tttacctgca tatttcgctt aatatagaga ttcatgcgtg gtggaacgac atgtaacgtt
      241 ttaggaaaag gttgcggtag atctcgtggt cgactgacat tagaaatacc gtacttcttc
      301 tttgcagctg gtgaagaagc taaaatcaac cctgatcctg tattatccgc tcgactcatg
      361 acgactaatt cagtcgttaa tggatctaaa ttcctttcta tacattcgac acttgcgtaa
      421 aagcttttca tatcgatcaa aaagtaatca tttactggct cttttgaata atccatcata
      481 aataacactc cattcttcca cattatacaa acatacgttc gtttttgtaa agaagaatcg
      541 attaaatgta caaaaaaaga agcataggta agttatttcc ctacttcttc tgttgatttg
      601 taaaatcaac acattgtatc actttattaa atggatattt actattagtc ttgtgaaaat
      661 ttgaaaataa gtgtcgtatt atccagtttt gcttagttat attagaaaaa aa
//
LOCUS       DAZKOU010000238          709 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000238 DAZKOU010000000
VERSION     DAZKOU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.248"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..600
                     /locus_tag="ACG827_002985"
     CDS             <1..600
                     /locus_tag="ACG827_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0485025.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          200 a          140 c          180 g          189 t
ORIGIN      
        1 accatgaaaa aagaatttcc gatcccgatt tcttggacaa cagatgtcaa tgcggctgcc
       61 tatggtgagt atgtcttcgg cagtggcaaa ggattgagca gtgtcgttta ctatacgatc
      121 ggtacgggtg ttggcggcgg tgccttacaa gaagggcgct ttatcgaagg gtttagtcat
      181 ccagaaatgg ggcatatgct cgtcgtccct catccaaaag atgactttgc tggcagctgt
      241 ccatttcatg gcaactgttt agaagggatg gccgctggac ccgccatcga aaaacgctta
      301 gggaaaaaag gacaagaggt tgcagaagat gatccttatt gggaaatcga agcctcctat
      361 attgctcaat gtgcctacaa tacaaccttg atgttttctc cagatgtgat catttttggc
      421 ggtggtgtga tgaaacaacg ccatatgctg caaaacgtcc acgacgcatt tgcaaaattg
      481 gtcaatgggt atgtcgaaac acctgagtta agcaaatata tcgtgacccc agcattagaa
      541 gacaacgctg gaacgctcgg ttgtttggca ttggctcgag aagcggtctt acattcataa
      601 acgaaaaaag gctcttcgac atttagtgtt ggtagagaaa gatacaactg aaaatttagt
      661 cataaccagt taggcagagt gcttaactgg tttttgtttt acttggaag
//
LOCUS       DAZKOU010000239          701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000239 DAZKOU010000000
VERSION     DAZKOU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.249"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          239 a           92 c           96 g          274 t
ORIGIN      
        1 aaaaggcaaa gatattttca aaacattttt tcttgatgtg atatccttgt cttttttgta
       61 atgtttttaa cttattgctt ttgcaaaatt tatttacctg ttttctggat caacgatagt
      121 gtatattatg ctatccttgt ttggatttta ggacgtttaa agaggcactt ttttacataa
      181 cttaatccat agacctttta agcatgctgt gagataataa taaagaagag tttaaagcgc
      241 accccaaacc acttccccat aagtgtatta cgctttaaac tcttttatat ttgaattcac
      301 taagaagcat accatatttt tgaaaaaaag tgagaaaaaa ggcttataat taggatttag
      361 agtaattaat tagatgttta ttttatagga ctgatattat agattacaaa tagtatcgta
      421 tttcgcaatc ttaaaattat cttcttacta tgaataagat aataaaattt gtatttgtcc
      481 tttttaggta acttatttta atctatatac ctttctggat caacgaaagt atactttata
      541 tagtcataac gccgatggta actatgcact gtaacgataa tgctgctaaa atatcttttt
      601 acgaaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
      661 atttaatatt taattaggag ggaatatttt gcttttttat a
//
LOCUS       DAZKOU010000240          692 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000240 DAZKOU010000000
VERSION     DAZKOU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.250"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG827_002986"
     CDS             132..344
                     /locus_tag="ACG827_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485026.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>692
                     /locus_tag="ACG827_002987"
     CDS             506..>692
                     /locus_tag="ACG827_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0485027.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVL"
BASE COUNT          246 a          103 c          122 g          221 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct ag
//
LOCUS       DAZKOU010000241          665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000241 DAZKOU010000000
VERSION     DAZKOU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.251"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..584)
                     /locus_tag="ACG827_002988"
     CDS             complement(42..584)
                     /locus_tag="ACG827_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0485028.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
BASE COUNT          220 a          134 c          105 g          206 t
ORIGIN      
        1 aaaatttaat ttagatatta gacagttttt aagtttgtga gttattttcc ttgtcgtttt
       61 ctcacttttt tagtccagaa accacctgaa atatattttg gctcataaga aatgataaaa
      121 gcacgctctt cgatttcttt gatcaggcga tacagttcac gttcgctttt tctaggagaa
      181 agtatttcga gaactagccg ctcaccttcc agccccattg cagaagtctg tgtcacacca
      241 taaccgtgtt ctctcagtat acccggcaaa cttttatctt ctgcgctatt tgctggcaaa
      301 atcgttgtga ccatgatgta tcctaatgcc aatttgtctt caatcaaaat tccgacactg
      361 atacctactg cgtaacctaa cgcatagaca aataaattca gtggattatc taaacgatct
      421 aaaacaaggg ataagccgac gatataaatc gtgatctcga ccatactgac aagtggagca
      481 atcagtcgat agcctttcat tgtcaacatg aaacgtaacg tgttcagagt gatataagca
      541 aaattaatga ggaagatcat ccccaacatt tttggatcaa tcatttgtaa aactcctttc
      601 atttatataa caacaatatc aaaaaaaatc gtaagtgaag aaaaattgaa actttataca
      661 tacaa
//
LOCUS       DAZKOU010000242          656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000242 DAZKOU010000000
VERSION     DAZKOU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.252"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..452)
                     /gene="tnpB"
                     /locus_tag="ACG827_002989"
     CDS             complement(138..452)
                     /gene="tnpB"
                     /locus_tag="ACG827_002989"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0485029.1"
                     /translation="MCGKTDLRKGIDGLASLIQQEYQLELYEDAVFLFCGNRQDRFKL
                     LYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLSIDQPRAILPGKKG
                     VF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAZKOU010000243          603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000243 DAZKOU010000000
VERSION     DAZKOU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.253"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..170)
                     /locus_tag="ACG827_002990"
     CDS             complement(<1..170)
                     /locus_tag="ACG827_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0485030.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFIL"
     gene            complement(246..392)
                     /locus_tag="ACG827_002991"
     CDS             complement(246..392)
                     /locus_tag="ACG827_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFQ0485031.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(496..>603)
                     /locus_tag="ACG827_002992"
     CDS             complement(496..>603)
                     /locus_tag="ACG827_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0485032.1"
                     /translation="FTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
BASE COUNT          216 a           98 c           65 g          224 t
ORIGIN      
        1 tttaatataa aataacttcc gattataggt atagctgcgg aaacatcagt aaataattca
       61 acaaaaaaag tgagtggtat caataaacat acaaaaagat attttctttt gttaagatca
      121 gtattagtca tataaaataa gagaaaaaag aaaaagtttc ccacgaacat tcttaataga
      181 taagcttttg tatcgatagt aatcaagaga tcccctcctg attttaggat atcataaaaa
      241 aaattttagt tacacttttt aaataatgaa aaaacacatt ccactaaatt tgatgccggt
      301 tttccttgag gttttgttcc agcacctcct ttaatttttt taagctcttg atctgaacat
      361 tgtttagcat ttagtctatt tttttcttcc attacgattc cccctacaaa ctataaatac
      421 ttttatgtca ctgattcaat atacctattt ttctaatcaa aaaattttga gacatattta
      481 atattataag tatcattaaa attgagattt atctccataa tctgctcgat ataaaccatt
      541 tattccagca gttcttccaa tttcactcaa atttcttatt aattcctctt gatattcagt
      601 gaa
//
LOCUS       DAZKOU010000244          593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000244 DAZKOU010000000
VERSION     DAZKOU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.254"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..580
                     /locus_tag="ACG827_002993"
     CDS             <1..580
                     /locus_tag="ACG827_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0485033.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DAZKOU010000245          585 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000245 DAZKOU010000000
VERSION     DAZKOU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.255"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..>585
                     /locus_tag="ACG827_002994"
     CDS             20..>585
                     /locus_tag="ACG827_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFQ0485034.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          205 a          101 c          116 g          163 t
ORIGIN      
        1 gatagcagga ggaatgacga tgaatcattt taaaggaaag caatttcagc aggatgtgat
       61 tattgtagcc gtgggctact atcttcgtta taaccttagc tatcgtgaag ttcaagaaat
      121 cttatatgat cgtggcatta acgtttctca tacgacgatt tatcgttggg tgcaagaata
      181 tggcaaacta ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt attcatggaa
      241 aatggatgaa acgtacatca aaattaaagg aaaatggcat tatttgtatc gagccatcga
      301 tgcagatggt ttaaccttgg atatttggtt acgtaaaaaa cgggacacac aagcagccta
      361 tgcttttctt aagcggttag tgaagcagtt tgatgaaccg aaggttgtag tcacagataa
      421 agccccctct attacaagtg cctttaagaa actaaaagaa tacggctttt atcaagggac
      481 agaacatcgt accattaaat acctgaataa tttgattgaa caagaccatc gtccagtaaa
      541 gagacgcaat aaattctatc gaagtttacg cactgcctct accac
//
LOCUS       DAZKOU010000246          582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000246 DAZKOU010000000
VERSION     DAZKOU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.256"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>582)
                     /locus_tag="ACG827_002995"
     CDS             complement(37..>582)
                     /locus_tag="ACG827_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0485035.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          166 a          110 c          100 g          206 t
ORIGIN      
        1 aatccactcc ttttttcatg ctctaaaatg atatttttac acaactgtta cggttgtttt
       61 acgaaattgg acgggacttt gccagtttaa tttctgtttt atccgttcat tattgtagta
      121 atagatatag ctatcgatgg ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg
      181 aaaaatctct tgttttaata agccaaaaaa attctccata agtgaattat ctaagcagtt
      241 ccctttccga gacatgcttt gaaagatttt gtactccttc aatttgtttc tataagcttt
      301 catttgatac gcccaacctt gatctgagtg aaacgtacga cgatagggac aatcgttagt
      361 ggtctggata gcttcctcca aaccttccat taccgctaat gcattgggct tttcagagat
      421 acgataggat aaaatctcac tattgaacat atccataaaa ggatctagat acaatttctt
      481 ttgacggata atgcctttgg catccacttc atggtattta aattcagttg tatccgtcgt
      541 gattttctga tggcagatac tggtgtagaa acgacgatgg at
//
LOCUS       DAZKOU010000247          580 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000247 DAZKOU010000000
VERSION     DAZKOU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.257"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..398
                     /locus_tag="ACG827_002996"
     CDS             141..398
                     /locus_tag="ACG827_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485036.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          243 a           73 c           80 g          184 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaaat tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 acaaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct
//
LOCUS       DAZKOU010000248          554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000248 DAZKOU010000000
VERSION     DAZKOU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.258"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..528
                     /gene="spxA"
                     /locus_tag="ACG827_002997"
     CDS             130..528
                     /gene="spxA"
                     /locus_tag="ACG827_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0485037.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          196 a           86 c          110 g          162 t
ORIGIN      
        1 tttttaaaaa aattatgaaa aaaaggtagc tatttaaaag ttgattatgt ataatagaaa
       61 cagatgggag attattatga tcttctgtta caaaaaagtg attcttatca cgaaaaggag
      121 tggtgcacgt tgttaacatt atatacttcc ccaagttgta cttcctgcag aaaggctcgt
      181 gcgtggttgc aagaacatca gattccattt gttgaacgta atattttttc agaaccattg
      241 aacagttctg aattaaaagc gattttacaa atgacagaag acggaacaga agaaatcatc
      301 tcgacacgtt ccaaagtatt ccaaaaactg aacatggatt tagatgatct gccgttacaa
      361 gaattattgg aacttgtgca aaacaatcct ggattgcttc gccgtccaat catgattgat
      421 gataaacgtc tgcaagttgg ttttaatgaa gatgaaattc gtcggttcct accgcgcgat
      481 gtacgacaat tagaattacg tcaagcacaa ttgatggctg gattatagaa ggataatacg
      541 aaacttggaa aaga
//
LOCUS       DAZKOU010000249          537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000249 DAZKOU010000000
VERSION     DAZKOU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.259"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>537
                     /locus_tag="ACG827_002998"
     CDS             <1..>537
                     /locus_tag="ACG827_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0485038.1"
                     /translation="DDFRSIITSMVMTYMFSFEGIKGYGYVVFQIMLDSLFLLMFIWL
                     YRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQVFDHFVFGVLIFLIIQMF
                     VVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLK
                     EIANQNHDSILEKEIKEL"
BASE COUNT          186 a           55 c           86 g          210 t
ORIGIN      
        1 ttgatgattt cagatctatt attacttcca tggttatgac gtatatgttt tcatttgaag
       61 ggataaaagg gtatggttat gtagtattcc agataatgct tgattctttg tttttgctga
      121 tgtttatttg gttatataga aaattagcta taaataggat tgttgaacag tccagttcta
      181 aacttactgc tatattcatg atttatttac ttacaattgg tttatttgtt tcttatgttg
      241 ctcatcaata tcaagtattt gatcattttg tgtttggtgt cttaatattt ttaatcattc
      301 aaatgtttgt tgttctattt ttatttatta gaataactat taggcaacga gaacaatatg
      361 aacagcaatt gaaaagaaaa gaattggttt atctaaaaaa atatactgat tcactggaaa
      421 aagatcaaga aaaatttgcc agatttagac atgactataa aaatttattg ctgagtttaa
      481 aagaaatcgc taatcaaaac catgattcga tattggaaaa agaaataaaa gaattag
//
LOCUS       DAZKOU010000250          532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000250 DAZKOU010000000
VERSION     DAZKOU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.260"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          192 a           72 c           60 g          208 t
ORIGIN      
        1 aattttctac gttttccttt tcatatatat taaatattac ggaaaattat ataaactaat
       61 ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt taggtctatt
      121 taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc tttcgtactt
      181 aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac tagtatgata
      241 tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt cctaaaacct
      301 ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa catgaaatta
      361 tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa catttattga
      421 ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt gataataaag
      481 tactacctat taaaggtagt actttattta atttagtata aattaacata ct
//
LOCUS       DAZKOU010000251          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000251 DAZKOU010000000
VERSION     DAZKOU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.261"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..354
                     /locus_tag="ACG827_002999"
     CDS             <1..354
                     /locus_tag="ACG827_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0485039.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DAZKOU010000252          520 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000252 DAZKOU010000000
VERSION     DAZKOU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.262"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..508
                     /locus_tag="ACG827_003000"
     CDS             <1..508
                     /locus_tag="ACG827_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HFQ0485040.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          158 a           91 c          111 g          160 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtctaact ttaactgtta
//
LOCUS       DAZKOU010000253          518 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000253 DAZKOU010000000
VERSION     DAZKOU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.263"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..339)
                     /locus_tag="ACG827_003001"
     CDS             complement(37..339)
                     /locus_tag="ACG827_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0485041.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          177 a           92 c           71 g          178 t
ORIGIN      
        1 aatccgttcc gggctacctt ttttaggtag cctactttaa tctttatacc tttctggatc
       61 aacgaaagta tacttcaaat agtcataacg ccgatgatcg ctccgtgcgt ctggcacatc
      121 agtcacgata tcaaacaaaa aatacacatc tttcttcatt ctggtttttg ttgccggaat
      181 tttaaagtag ttcttattag agtaatagag attgattaat aagctatctt cgattgctaa
      241 aaagaaaact tctgaatccc acaccttata aaaatctttg acaaatctat tcgaaggatc
      301 aaatttaaac cataattgtg tttttccttc catcaacata atgttcacct caaaaagagt
      361 atacgaacta atgttctttt tgtaaagtag gaatatcatt gataatatgg atggaacata
      421 tatattaaaa agtgttcttt ttgaatgcta ttttgaatgc cattttcatt catttaacga
      481 aaatctgcga aaataaaaaa tgctcaaacc cttataaa
//
LOCUS       DAZKOU010000254          517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000254 DAZKOU010000000
VERSION     DAZKOU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.264"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..>517
                     /locus_tag="ACG827_003002"
     CDS             57..>517
                     /locus_tag="ACG827_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0485042.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          191 a           72 c          105 g          149 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaa
//
LOCUS       DAZKOU010000255          502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000255 DAZKOU010000000
VERSION     DAZKOU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.265"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          170 a           96 c           58 g          178 t
ORIGIN      
        1 aaaaataaga atgctaaatc actctacaat aacggcgaaa gcacaaaaaa taaaaaaatt
       61 attacgttat taaaaaaaca gatgaaagat caggttttat tagatctcat tctttcatca
      121 cttgaaggga tttaatgaac tactttttcc tctttgtcgt ttttatatat tcgtattcct
      181 tcaaacagta ggatagcctc tttcaatgct tgatatttga actgtccgtt acttaaaaat
      241 ttagatgtgc cgccctcttc ctagaacata ttctttttta ctttttcatt aattgacaga
      301 ccaatcgaac gaaactgtaa atttcgatag gataatgatt ggtcaaactc attcattttc
      361 aagacatctc gattcatatt caagatagga atctttttat caacatcact ttattcgtat
      421 tttcctctac cataaaagct tcacgctcca caaaattgtt ttatacacta ctattcagcc
      481 cattcaacga ccaatgaatt tt
//
LOCUS       DAZKOU010000256          496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000256 DAZKOU010000000
VERSION     DAZKOU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.266"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..327
                     /locus_tag="ACG827_003003"
     CDS             <1..327
                     /locus_tag="ACG827_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0485043.1"
                     /translation="KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQ
                     REGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRK
                     LSEVNN"
BASE COUNT          199 a           66 c           90 g          141 t
ORIGIN      
        1 aaaaaggcaa gtttaaaatc actaaaagat acatggcttg atttatcaga agataatcca
       61 tacagccaat tcttaattac tgtaatggct ggtgttaacc aattagagcg agatcttatt
      121 cggatgagac aacgtgaagg gattgaattg gctaagaaag aaggaaagtt taaaggtcga
      181 ttaaagaagt atcataaaaa tcacgcagga atgaattatg cggtaaagct atataaagaa
      241 ggaaatatga ctgtaaatca aatttgtgaa attactaatg tatctagggc ttcattatac
      301 aggaaattat cagaagtgaa taattagcca ttctgtattc cgctaatggg caatattttt
      361 aaagaagaaa aggaaactat aaaatattaa cagcctccta gcgatgccga aaagcccttt
      421 gataaaaaaa gaatcatcat cttaagaaat tcttagtcat ttattatgta aatgcttata
      481 aattcggccc tataat
//
LOCUS       DAZKOU010000257          473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000257 DAZKOU010000000
VERSION     DAZKOU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.267"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..429
                     /locus_tag="ACG827_003004"
     CDS             13..429
                     /locus_tag="ACG827_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFQ0485044.1"
                     /translation="MMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIP
                     ILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYIT
                     KTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          194 a           59 c           66 g          154 t
ORIGIN      
        1 attggagaga aaatgatgga tcaaaaagcg tttatcgaat ctattgattc tacaatcgat
       61 agattaaaaa aagaaattcg attttataat agagtaattg gtatcggaaa tattataaaa
      121 atagtcttat cagctagtat cccaatatta attgatcagg cttccgaaca caggtcttta
      181 ctattgattg tttcaattgc ttcagcaata attacaatta tacaaagtgg gatgtctgca
      241 tttaattatc aagataaagt acagacttcg acagagttat taatgaaaat tgaaaaagaa
      301 aagttgttat atataacgaa aacatcccca tataataaaa cagatgaaga aaatttccat
      361 ttaatcgtta caacccttca aactgaatta aatgatatta tctctgattt taatcaagtt
      421 aataactaaa aatttattta attagaaagt agtgcagata atactaattg tac
//
LOCUS       DAZKOU010000258          465 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000258 DAZKOU010000000
VERSION     DAZKOU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.268"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>465
                     /locus_tag="ACG827_003005"
     CDS             <1..>465
                     /locus_tag="ACG827_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0485045.1"
                     /translation="EGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEAT
                     QPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTK
                     YTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          190 a           68 c          109 g           98 t
ORIGIN      
        1 agaaggaaca agtattgaaa gtggaacaac agtctttgca gtgaaagacg gcgtaagctt
       61 accagaagat aaacttccag tgttgaaagc aaaagatggt tatacagatg cgaaatggcc
      121 agaggaagca actcaaccaa ttacagcaga tgatacagaa tttgtatcaa gtgcaacaaa
      181 actggatgat aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag tgactacaga
      241 attgaataag gaaccagacg catttgaggg aattaagaac aaagaagatt taccaaaaga
      301 tactaagtat acttggaaag aaaaagtaga tgttagcgca gctggaaata aaaaagggac
      361 tgttgtagtg acatatccag atggatcatc tgatgaagtt gaagtagatg ttacagtgac
      421 agacaatcgt tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DAZKOU010000259          460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000259 DAZKOU010000000
VERSION     DAZKOU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.269"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG827_003006"
     tRNA            complement(20..91)
                     /locus_tag="ACG827_003006"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG827_003007"
     tRNA            complement(113..200)
                     /locus_tag="ACG827_003007"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG827_003008"
     tRNA            complement(225..298)
                     /locus_tag="ACG827_003008"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG827_003009"
     tRNA            complement(312..382)
                     /locus_tag="ACG827_003009"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG827_003010"
     tRNA            complement(384..459)
                     /locus_tag="ACG827_003010"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAZKOU010000260          452 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000260 DAZKOU010000000
VERSION     DAZKOU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.270"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..205)
                     /locus_tag="ACG827_003011"
     CDS             complement(74..205)
                     /locus_tag="ACG827_003011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485046.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
     gene            complement(178..300)
                     /locus_tag="ACG827_003012"
     CDS             complement(178..300)
                     /locus_tag="ACG827_003012"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485047.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
BASE COUNT          151 a           83 c           65 g          153 t
ORIGIN      
        1 tggcaaaaat ttcaatcttt aaatggcgaa aaacaaagag cgtgagaccc tatccttatc
       61 tcacgctctt tgtctatttt tctaacaaat caagtatttc ttgaggagaa tcaataatta
      121 tatctacact ttgaaaattt tctatatttt tggctcccca tttagctaaa ccaaatgcta
      181 tcccacaagc tttcgcactt tgcatgtcat ataatgaatc accaatataa atcgtttctt
      241 attttttatg cccaattcat caattgcctt ttgtatgggg tccggaaaag gtttatgcaa
      301 cttagtatct gaagctgtaa tttgtatatc aaaatataga tttaacccgt ggtgctagtt
      361 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcatata
      421 gtagtctcaa aactcccaag aaatgaggac ta
//
LOCUS       DAZKOU010000261          437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000261 DAZKOU010000000
VERSION     DAZKOU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.271"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..437
                     /locus_tag="ACG827_003013"
                     /pseudo
     CDS             1..437
                     /locus_tag="ACG827_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          179 a           77 c           80 g          101 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattga
//
LOCUS       DAZKOU010000262          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000262 DAZKOU010000000
VERSION     DAZKOU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.272"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..355)
                     /locus_tag="ACG827_003014"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACG827_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAZKOU010000263          419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000263 DAZKOU010000000
VERSION     DAZKOU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.273"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>419
                     /locus_tag="ACG827_003015"
     CDS             <1..>419
                     /locus_tag="ACG827_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0485048.1"
                     /translation="EKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEY
                     GELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHH
                     YTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          154 a           76 c           70 g          119 t
ORIGIN      
        1 gaaaaagcga taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa
       61 tatgatcgca ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc
      121 aaaaccgaat atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa
      181 caacaaactt taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat
      241 ttattcgaaa aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt
      301 catcactata ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat
      361 gcctttaaat ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctactta
//
LOCUS       DAZKOU010000264          418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000264 DAZKOU010000000
VERSION     DAZKOU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.274"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>418
                     /locus_tag="ACG827_003016"
     CDS             <1..>418
                     /locus_tag="ACG827_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0485049.1"
                     /translation="YRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTK
                     GELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPI
                     TYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          140 a           77 c           71 g          130 t
ORIGIN      
        1 tatagagcgg tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc
       61 actcgcttaa gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag
      121 aagctcacca aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa
      181 ccagttagaa atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca
      241 acaaaaaaat cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt
      301 ccgattactt actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta
      361 tcggaagaag cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZKOU010000265          415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000265 DAZKOU010000000
VERSION     DAZKOU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.275"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG827_003017"
     CDS             complement(<1..339)
                     /locus_tag="ACG827_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0485050.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAZKOU010000266          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000266 DAZKOU010000000
VERSION     DAZKOU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.276"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACG827_003018"
     rRNA            <1..>411
                     /locus_tag="ACG827_003018"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAZKOU010000267          391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000267 DAZKOU010000000
VERSION     DAZKOU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.277"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..>391
                     /locus_tag="ACG827_003019"
     CDS             149..>391
                     /locus_tag="ACG827_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0485051.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT          142 a           62 c           74 g          113 t
ORIGIN      
        1 attattagaa ttttaaaaaa tgataaaaat tcaaaaagaa aaaatagtag ttatttactt
       61 gtcatgttac taaataaaaa aataatttaa atatgatata tagtcattta tttagtgatt
      121 tcattctaaa ataaagggag aaatgtctat gctatacggt gcaattgaag caggcggcac
      181 aaaatttgtg tgtgccattg gcgatgaaga aatgacgatc aaggaaagag tgagctttcc
      241 gacaacgaca cctgaggaaa caatgccttt agtcattgac ttcttcaaac aataccaagc
      301 agatttagca gggattggga ttggctcatt tgggcctatc gatatccatc gcgattccgc
      361 cacttatggg tacattacct cgaccccgaa a
//
LOCUS       DAZKOU010000268          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000268 DAZKOU010000000
VERSION     DAZKOU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.278"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG827_003020"
     CDS             <1..>386
                     /locus_tag="ACG827_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0485052.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKOU010000269          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000269 DAZKOU010000000
VERSION     DAZKOU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.279"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG827_003021"
     CDS             <1..>386
                     /locus_tag="ACG827_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0485053.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKOU010000270          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000270 DAZKOU010000000
VERSION     DAZKOU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.280"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACG827_003022"
     CDS             complement(<1..272)
                     /locus_tag="ACG827_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485054.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          106 a           69 c           52 g          141 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataa
//
LOCUS       DAZKOU010000271          366 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000271 DAZKOU010000000
VERSION     DAZKOU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.281"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>366
                     /locus_tag="ACG827_003023"
     CDS             22..>366
                     /locus_tag="ACG827_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0485055.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTG"
BASE COUNT          126 a           73 c           75 g           92 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acggga
//
LOCUS       DAZKOU010000272          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000272 DAZKOU010000000
VERSION     DAZKOU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.282"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACG827_003024"
     rRNA            complement(<1..>362)
                     /locus_tag="ACG827_003024"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAZKOU010000273          352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000273 DAZKOU010000000
VERSION     DAZKOU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.283"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..251
                     /locus_tag="ACG827_003025"
     CDS             12..251
                     /locus_tag="ACG827_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0485056.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT          144 a           38 c           78 g           92 t
ORIGIN      
        1 gaaagaagtg agtgattgtg ggaaatttag gcgcacaaaa agaaaaacga aatgatacac
       61 caatcagtgc aaaaaaagat ataatgggag ataagacggt tcgtgttcgt gctgacttgc
      121 accatatcat aaaaatcgaa acagcaaaga atggcggaaa cgtaaaagaa gttatggaaa
      181 taagacttag aagcaaactt aagagtgtgt tgatagtgca ttatcttaaa attttgtata
      241 ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca
      301 tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa ca
//
LOCUS       DAZKOU010000274          344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000274 DAZKOU010000000
VERSION     DAZKOU010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.284"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>344
                     /locus_tag="ACG827_003026"
     CDS             <1..>344
                     /locus_tag="ACG827_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFQ0485057.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DAZKOU010000275          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000275 DAZKOU010000000
VERSION     DAZKOU010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.285"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>321
                     /locus_tag="ACG827_003027"
     CDS             <1..>321
                     /locus_tag="ACG827_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFQ0485058.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DAZKOU010000276          315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000276 DAZKOU010000000
VERSION     DAZKOU010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.286"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>315
                     /locus_tag="ACG827_003028"
     CDS             <1..>315
                     /locus_tag="ACG827_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485059.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTT"
BASE COUNT          109 a           62 c           72 g           72 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgac
//
LOCUS       DAZKOU010000277          311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000277 DAZKOU010000000
VERSION     DAZKOU010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.287"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..>311
                     /locus_tag="ACG827_003029"
     CDS             63..>311
                     /locus_tag="ACG827_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0485060.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLE"
BASE COUNT           98 a           59 c           60 g           94 t
ORIGIN      
        1 agaaaagaca ccctgaaatc ctttagaatt aatttggcta ccaattcata ggaggattca
       61 agatgtctca tactcagctt atcaaagata ccctaaatat tctagaccta aatatctatt
      121 tcgaggaaaa ttgtttaact aaagaaaaat acaaagggca aatttgcttg atttacaaag
      181 gaactcttct gtacaagcca gaagagtgtt cccattgtct ttgtgtcgtc ccctctagga
      241 tcattcgttg ggggacgaca accgttcggt tgttactgaa tgatgtgtct gagtatcgca
      301 cttatctgga g
//
LOCUS       DAZKOU010000278          307 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000278 DAZKOU010000000
VERSION     DAZKOU010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.288"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG827_003030"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG827_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0485061.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           96 a           45 c           50 g          116 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttat
//
LOCUS       DAZKOU010000279          301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000279 DAZKOU010000000
VERSION     DAZKOU010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.289"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..301
                     /locus_tag="ACG827_003031"
                     /pseudo
     CDS             1..301
                     /locus_tag="ACG827_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           60 c           57 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 g
//
LOCUS       DAZKOU010000280          295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000280 DAZKOU010000000
VERSION     DAZKOU010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.290"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          134 a           36 c           46 g           79 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgtt
//
LOCUS       DAZKOU010000281          286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000281 DAZKOU010000000
VERSION     DAZKOU010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.291"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          132 a           35 c           24 g           95 t
ORIGIN      
        1 cattaaaatt aacttattta ataagatact actgtgttaa tctgaaaaaa attactttaa
       61 agtagttaca tactacctgt taatttagat actaccatta atacttaaat tagatactac
      121 ctatatttat attaagtact accgttctaa gaataagata ataaaaaaat actaccaaat
      181 tgcaattaaa tactacttaa aatgtaaaaa ataagaacta cccaaattag gtagttctta
      241 ataagagaat atcagataaa tttgtacaag tcataaaaaa attaat
//
LOCUS       DAZKOU010000282          283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000282 DAZKOU010000000
VERSION     DAZKOU010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.292"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>283
                     /locus_tag="ACG827_003032"
     CDS             <1..>283
                     /locus_tag="ACG827_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0485062.1"
                     /translation="HKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNL
                     FKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           99 a           58 c           50 g           76 t
ORIGIN      
        1 accataaaag tgccgtcatc acactcgttg agcgactttc aaaagccatt atcgccttga
       61 aaccagaagg ccgtaaggca gttgatattg aaaattcgat taatgaatgg cttcaatccg
      121 tacccaaaaa tcttttcaaa tcaattacct ttgattgtgg aaaagaattc tctaattgga
      181 aaagtattag taatacgaat gatattgata tttatttcgc agacccagga acgccttccc
      241 aacggggatt aaatgaacat tcaaacggac tcctccgaaa aga
//
LOCUS       DAZKOU010000283          279 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000283 DAZKOU010000000
VERSION     DAZKOU010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.293"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          116 a           30 c           50 g           83 t
ORIGIN      
        1 aatgattcct ttaaggtgtc actaaaaatt ataaaaaata gtgttgacgc acttttgatg
       61 tcatgttata ataacgaagt cgtttggatg agatagctaa taaagcttga ttttatcgaa
      121 aaaaacttcg aaaaagttat tgacaaataa caataaagat ggtatgataa tcaagtcgct
      181 gctaatgagt ggcaaagacg tttagaaaaa ataaacaaag aagttgttga cattctcttc
      241 tgaatctgat aagattaaag agttgttaaa acaaaaaga
//
LOCUS       DAZKOU010000284          277 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000284 DAZKOU010000000
VERSION     DAZKOU010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.294"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          108 a           28 c           40 g          101 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaatta
//
LOCUS       DAZKOU010000285          269 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000285 DAZKOU010000000
VERSION     DAZKOU010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.295"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..260
                     /locus_tag="ACG827_003033"
     CDS             <1..260
                     /locus_tag="ACG827_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485063.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
BASE COUNT           97 a           27 c           48 g           97 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaac
//
LOCUS       DAZKOU010000286          266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000286 DAZKOU010000000
VERSION     DAZKOU010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.296"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..>266)
                     /locus_tag="ACG827_003034"
     CDS             complement(34..>266)
                     /locus_tag="ACG827_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0485064.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaata
//
LOCUS       DAZKOU010000287          254 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000287 DAZKOU010000000
VERSION     DAZKOU010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.297"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           86 a           47 c           18 g          103 t
ORIGIN      
        1 ctccaatatc aagtatacac ttatcgcttc caattacaca tgatcatatt agagtgtatt
       61 atcacattct ctaatattca atttattttt cctaaaatat tcgaaataat cgtcttgata
      121 acgagtaagt atttttatac gattgataac atagtaacaa tatttttaat tatagtaaaa
      181 aatcatctgc ctttttcatc cacctaatct tatttttcat tttattcaac atccttaaat
      241 aaacttccgt tcct
//
LOCUS       DAZKOU010000288          252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000288 DAZKOU010000000
VERSION     DAZKOU010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.298"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..>252)
                     /locus_tag="ACG827_003035"
     CDS             complement(32..>252)
                     /locus_tag="ACG827_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0485065.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DAZKOU010000289          232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000289 DAZKOU010000000
VERSION     DAZKOU010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.299"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>232)
                     /locus_tag="ACG827_003036"
     CDS             complement(<1..>232)
                     /locus_tag="ACG827_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0485066.1"
                     /translation="LLNDVSNYKTILRINKQRFKCKHCGKTFLAEDSVSDRRCSIARR
                     VKQAILELLSEPLSMSLIARMKHISPTTVIRIL"
BASE COUNT           70 a           42 c           53 g           67 t
ORIGIN      
        1 aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcgatca aagacataga
       61 aagaggttcg cttaatagct caagaatggc ttgtttgact ctgcgagcaa tggagcagcg
      121 acgatcacta actgaatctt ctgctaaaaa agtctttcca cagtgtttac atttgaagcg
      181 ctgtttgtta atccgcagaa tggtcttgta gttactgaca tcattcaata ag
//
LOCUS       DAZKOU010000290          232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000290 DAZKOU010000000
VERSION     DAZKOU010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.300"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           90 a           20 c           44 g           78 t
ORIGIN      
        1 aatatgtctt taaaaatgga atggttgcaa ttcttagcga tatatacgat gtaattgtcc
       61 tcttaaaaaa gtatggaaaa ataattgtat tgggtgttat gtataattca tggtgaactg
      121 agactaatga gaagaaaaat tcatattgtt atgagtgaga ttgtatcaga tgagaatgat
      181 aaattaaata ggtgcataaa agcattacac ttaaaataca ttgttgctta ca
//
LOCUS       DAZKOU010000291          224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000291 DAZKOU010000000
VERSION     DAZKOU010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.301"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACG827_003037"
     CDS             <1..>224
                     /locus_tag="ACG827_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFQ0485067.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZKOU010000292          220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000292 DAZKOU010000000
VERSION     DAZKOU010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.302"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..175)
                     /locus_tag="ACG827_003038"
     rRNA            complement(<1..175)
                     /locus_tag="ACG827_003038"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           48 a           60 c           49 g           63 t
ORIGIN      
        1 cctgtttcca agtgttatcc ccttctgatg ggcaggttac ccacgtgtta ctcacccgtt
       61 cgccactcct ctttttccgg tggagcaagc tccggtggaa aaagaagcgt tcgacttgca
      121 tgtattaggc acgccgccag cgttcgtcct gagccaggat caaactctca taaaaagttc
      181 gaacaatctt gcgattgtta agctcaattt gtttgctagc
//
LOCUS       DAZKOU010000293          220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000293 DAZKOU010000000
VERSION     DAZKOU010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.303"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>220
                     /locus_tag="ACG827_003039"
     CDS             <1..>220
                     /locus_tag="ACG827_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0485068.1"
                     /translation="SFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRS
                     HASIEDKMSFICADGETGQLIDVLPTRKL"
BASE COUNT           66 a           40 c           45 g           69 t
ORIGIN      
        1 tcttttattg cgaaaaattg tcaggtatct ttgaccaccg ttattcgtac gttgaaagag
       61 tttaaaagct acttaccaaa gcaatctaag aggattctcc ccagagtatt gatggttgat
      121 gaatttcgtt cgcacgcttc catagaagat aaaatgagct ttatttgcgc agatggcgaa
      181 acaggacaat taattgatgt cttgcctacg cgtaaattgc
//
LOCUS       DAZKOU010000294          206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000294 DAZKOU010000000
VERSION     DAZKOU010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.denovo.304"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>206)
                     /locus_tag="ACG827_003040"
     CDS             complement(<1..>206)
                     /locus_tag="ACG827_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0485069.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT           56 a           42 c           37 g           71 t
ORIGIN      
        1 caatgctgat attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag
       61 ccgtccgtaa taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc
      121 tcattccaaa cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg
      181 ccgtcttctc gtataccaat ggcaat
//
LOCUS       DAZKOU010000295        21116 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.guided.052, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000295 DAZKOU010000000
VERSION     DAZKOU010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.guided.052"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..587)
                     /locus_tag="ACG827_003041"
     CDS             complement(<1..587)
                     /locus_tag="ACG827_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0485070.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRG"
     gene            846..2348
                     /locus_tag="ACG827_003042"
     CDS             846..2348
                     /locus_tag="ACG827_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0485071.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            2350..3087
                     /locus_tag="ACG827_003043"
     CDS             2350..3087
                     /locus_tag="ACG827_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0485072.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(3258..4451)
                     /locus_tag="ACG827_003044"
     CDS             complement(3258..4451)
                     /locus_tag="ACG827_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0485073.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(4530..4736)
                     /locus_tag="ACG827_003045"
     CDS             complement(4530..4736)
                     /locus_tag="ACG827_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0485074.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(4729..5871)
                     /locus_tag="ACG827_003046"
     CDS             complement(4729..5871)
                     /locus_tag="ACG827_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0485075.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            6383..7018
                     /locus_tag="ACG827_003047"
     CDS             6383..7018
                     /locus_tag="ACG827_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0485076.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            7172..7249
                     /locus_tag="ACG827_003048"
                     /pseudo
     CDS             7172..7249
                     /locus_tag="ACG827_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            7563..7610
                     /locus_tag="ACG827_003049"
                     /pseudo
     CDS             7563..7610
                     /locus_tag="ACG827_003049"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7626..9545
                     /gene="tet(M)"
                     /locus_tag="ACG827_003050"
     CDS             7626..9545
                     /gene="tet(M)"
                     /locus_tag="ACG827_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0485077.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            9664..9831
                     /locus_tag="ACG827_003051"
     CDS             9664..9831
                     /locus_tag="ACG827_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0485078.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(9891..10244)
                     /locus_tag="ACG827_003052"
     CDS             complement(9891..10244)
                     /locus_tag="ACG827_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0485079.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            10748..11167
                     /locus_tag="ACG827_003053"
     CDS             10748..11167
                     /locus_tag="ACG827_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0485080.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            11193..11399
                     /locus_tag="ACG827_003054"
     CDS             11193..11399
                     /locus_tag="ACG827_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0485081.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(11627..11800)
                     /locus_tag="ACG827_003055"
     CDS             complement(11627..11800)
                     /locus_tag="ACG827_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485082.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            11812..12441
                     /locus_tag="ACG827_003056"
     CDS             11812..12441
                     /locus_tag="ACG827_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0485083.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(12523..13689)
                     /gene="dnaJ"
                     /locus_tag="ACG827_003057"
     CDS             complement(12523..13689)
                     /gene="dnaJ"
                     /locus_tag="ACG827_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0485084.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(13840..15669)
                     /gene="dnaK"
                     /locus_tag="ACG827_003058"
     CDS             complement(13840..15669)
                     /gene="dnaK"
                     /locus_tag="ACG827_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0485085.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(15720..16283)
                     /gene="grpE"
                     /locus_tag="ACG827_003059"
     CDS             complement(15720..16283)
                     /gene="grpE"
                     /locus_tag="ACG827_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0485086.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(16377..17420)
                     /gene="hrcA"
                     /locus_tag="ACG827_003060"
     CDS             complement(16377..17420)
                     /gene="hrcA"
                     /locus_tag="ACG827_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0485087.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(17654..18819)
                     /gene="hemW"
                     /locus_tag="ACG827_003061"
                     /pseudo
     CDS             complement(17654..18819)
                     /gene="hemW"
                     /locus_tag="ACG827_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(18835..19062)
                     /locus_tag="ACG827_003062"
     CDS             complement(18835..19062)
                     /locus_tag="ACG827_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0485088.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            19546..20153
                     /locus_tag="ACG827_003063"
                     /pseudo
     CDS             19546..20153
                     /locus_tag="ACG827_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            20326..21039
                     /locus_tag="ACG827_003064"
     CDS             20326..21039
                     /locus_tag="ACG827_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485089.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         6257 a         4092 c         3902 g         6865 t
ORIGIN      
        1 ccgcgctgaa aatctatatc atttctttcg aattggatac tgactaagtc acgaatcaac
       61 tgattgcgat ctagttctgc tccctgtcga atcgaaacta cttgtttttg gtactcaaaa
      121 ggcgagccta atccaaaaat acatgaaacg gatgcaatga cgatcacatc atttctttct
      181 aataatgaac tagtagccga atgtcgtaat ttgtcgatct catcatttac acttgaatct
      241 ttttcgatat acgtatcgct tgaaggcaca taagcttcag gctgataata atcataataa
      301 gaaacaaaat attcaacagc attgttaggg aaaaattcct tgaattcccc gtacagctga
      361 ccagccaatg ttttgttatg ggcaataatc agtgttggtt tattcacttt ctcaataaga
      421 ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta ataagatttg tgcttttttc
      481 ccgccgacta cgccatccac taattggtta atcgcttcag gctgatcacc agccggttgg
      541 tatttcgaga caagctcaaa gtgtcgggag gtatctctct caatcattct tcgtcctcct
      601 tttcattcat tccttttcat tttacgacca ataaaaagaa aaggaaagca aaggcattcc
      661 ttactaaaaa attataccac accgaacata ctttcgccac ttttttatct tattatacat
      721 gttatgtttt ataacataaa atgttagaaa ctcgcatttt tttcattttt tcgagcttac
      781 agtcttgtct gattcagcac ttttcgccta taataataaa atattggaaa tttaggaggt
      841 tgattatgaa aagaaagcat ttatttcact catttgtttt cttaatgaca ggacttgtga
      901 cacttgcgtt tggtcatgtt gcacaagctg acaacactac aacggcagag tcaagcacaa
      961 gcgagacaat gcaaaaaata gagcccaaaa aagacgtata cgtaattgcc agtgactctg
     1021 cttttgctcc atttgaattt caaaacgcaa agggcgagta cgaaggcatc gatgtaaaat
     1081 tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg aatggactac cgtggtttct
     1141 ctgctgcttt gcaagctttg gaatcaggac aagcagacgg aatgattgcc ggaatgactg
     1201 taacagatga acgaaagaaa agctatgatt tttctgattc ttactttgat agcgggatcc
     1261 aattagctgt caaaaaagga gatacttcga tcaagtctta tgaagatttg aaaggaaaaa
     1321 cagtcggagc aaaagttggt accgaaagtg ctgaattttt aagcgaccat gaatctgaat
     1381 atggctattc tatcaaatat tttgatgctg ctgatcaatt gtacgacggg ttaaaagttg
     1441 gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg ttatgcgatc aaacaaggac
     1501 aagaattgga aacaccgatc aaaaaagaat ctggcggaaa atatgcattt gccgttaaaa
     1561 aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg attgaaagaa ttaaaaagaa
     1621 caggcgaata cgataagatt attaaagaat atgtagcgga tggttcagaa acttccagtg
     1681 agtcttccac tgctgatgaa tcaacatttc ttggacttat ccaaaacaac tatcaagcat
     1741 tgttgaaagg actttggaga acaattctcc taactttgat ctcctttgct ttggctatag
     1801 taatcggtgt gatttttgga ttattcagtg tagcaccaat acgcgctctt cgcacaattg
     1861 ctagtattta tgtcgatatc atccgaggaa ttccaatgat ggttcttgcg ttctttattt
     1921 tctttgggtt gcctggtatc gttggattta cgattcctga cttccttgct ggtgttatca
     1981 ccttgactct taacgcaagt gcttatatcg cagagattgt tcgaggcggg atcaacgccg
     2041 ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct ttcttataat cggacaatgc
     2101 aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc atcattcgtc aatcagttcg
     2161 ttatctcatt aaaagacaca acgatcattt ccgcaatcgg tgtagtcgaa ttgctgcaaa
     2221 ctggtaagat cattgttgca cgtacaacac aaagtacgta tgtttacttg atcattgcga
     2281 tcatgtactt gattttgatc acagcattga caaaattagc aaaagtttta gaaaagaagg
     2341 tgaagtaaga tggcagaaaa aatcttagtt gagcatttag tcaaaaaata cggcgataac
     2401 acagtattaa acgacatcaa tgtatccatc aacgaaggag atgtcgtctg tatcattggc
     2461 ccttcaggtt caggaaaaag tacattcttg cgttgcttga atcgtttaga agaaccttct
     2521 gacggtgaaa ttattattga cggcgcccac ttgatggata aaagcacaga tatcaacttg
     2581 gtgagacaac atatcggaat ggtattccaa cactttaacc tattccctca tctttctatt
     2641 ttggaaaaca tcattttagc accgatggac ctgaagaaag aatctagaga agaagcagag
     2701 aaaaaagcaa tcaaattact gcagacagtt ggtctagaag ataaaaaaga tatgtatcct
     2761 gaaatgcttt ctggtggaca aaaacagcgt gtggctattg ctcgtgctct agcgatgaac
     2821 cctgacatca tgctttttga tgagcctact tctgcactcg atcctgaaat ggtcggtgat
     2881 gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga cgatggttat cgttacgcac
     2941 gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt tcattgacga cggaaacttt
     3001 ttagaagatg gaacgcctca acaaatcttt gaaaatccgc aaaatgaacg gacaaaagat
     3061 ttcttgaata aagtattaaa tatttaaaat aaattatata tgtctttttg actatctttt
     3121 tatatcttct taaatgtccg caaagcctta ttttataggc tctacgggca ttttgctttt
     3181 gtggtaaacc tcacatatct aggtctatct tcttatattt tcactatcaa gcgtggttaa
     3241 aatcgtggta aatacttcta tgcaattaga cgctgaacct ctgattttgc ggtatctatg
     3301 gacgcatgag cataccagtt cattgtgata ctgatatttg aatgccccat gatatactgt
     3361 aaatcttttg ggttcatgtt cttgcttgcc agtcttgtgc agaatgtatg gcgtagcgta
     3421 tgcggtgtga tatgtggcaa tggattgtcc ttgtggtgct tgttgtattt ctttaccata
     3481 cggataaata aggcgttata atcaatcgca actttgggct tgcccttatg attgacaaat
     3541 aggaagttgc cccgtccgtc tatcacaaat ggttctgcct ttggacgttt tttcataacc
     3601 tgttgaaatg cctgtatcgt ttctttgctt aatggtactt gccttattcc gctttttgtt
     3661 ttaggcgttt caatataata gccctgttcc ttgctcttta gtaactggtg gtcgataatc
     3721 acaacttcat tcttgaaatc aatgtcggct actgtcagtc cgcacagttc cgaaatacga
     3781 agtcctgtct ttaacagtat cagcacatca tcataatgct tgtgatacac attgtccgtc
     3841 ttgatgaatg agagtaaggc ttgttcctgt tcctctgtca atgcgacttt ctttttggta
     3901 tcattttcta ggacttcact caacttgaaa tcaaagggat ttttcctcac ataatcatct
     3961 tgaatggcta tgtagaatga cgcttttaac gagcgtttat ggttgttaat ggtattgtag
     4021 gaaaagcctt tgtctttcat gcgtaacgcc cattccttag cgtcagaggg tttgattgta
     4081 tcaatgctcc tagcacctaa cttgtcctct ttcaataacc gcatgagttg ttcccgttgt
     4141 ttctgtgtgc ttttcttcac atttgccctc tgtgcgttct gtttggcata aagttggcaa
     4201 agcgtcattt tcttgccagt gctgtcgata ccgtcctcaa tatcccgtct tatctgctgt
     4261 tccagttctc gaagtgaggg tttttcccgt tttccctttg gtgtcgggtc tgtgggtgtc
     4321 aatctccaag catacacata ttttgtgttt ccaaatgcgt ctacatattt atataagtat
     4381 tttctgtctg ttcgttggct ctctccagta tgcaggatac gacctttgtt atcccgtctt
     4441 ttttctttca tggtgtctgc tcctttcctt gttggaaaga gccttgatat gacttgtgtt
     4501 catcataaca catacaaggc tcatttgcat tagatagcgt ccaatttatc aatgacctgt
     4561 tcaaactgtc ggcgtttaat ctgtatgcgg ttgccattca taatgagcca gccagcgtcc
     4621 ttgttttcct ctgccaatcg tcttaacttg ttttctccga tacggaaata ctttgacgct
     4681 tcttcaatgg taagggtgta tttttcccat acgggaatat cctgtgcatt actcataaaa
     4741 caccccctca ctttcgatta cgtctgctat gtccgtacac atctgttcca caagcatttc
     4801 ctgcttttca gagaggtggg tacattcatc aatcattttc atgtagtttg agcctgtcag
     4861 cctgtcgatt tcctctatca tcacgattga cggcatgacc tgtctttcca gccattgcag
     4921 agtgcgttcc atattgacag gtacggggtt catttcaaaa ggtaaactgt catgggaaat
     4981 gaatatctcc cacagcggat agtcgggaaa ttgcacaaaa tcagtaataa ggtaaaacac
     5041 cagcccatga gggttatatg tcaataacaa ttcctcaact gcctgtactg cttttgtacc
     5101 acgcagacga atttcaaaac gattgataat atccgtatgt ttctttttgg ttgcctgttc
     5161 cttttgtttt tcatacaggc agaagtattt tgtgcttgcc tttgaaccga tataaagggt
     5221 gcttgctaaa ttacggtttt ttccgcttag ttttccaccc tgcacatttt cataattttt
     5281 acagcgacaa tccgcaccgc catttctgta tttttcagac aaaacgggaa tatccaacag
     5341 accacacata tcattgattg ccaagtcaaa acgtcggata acaccgtcac agtcaagaca
     5401 gcggtttaaa aatgaatacc aatctctgtt ctgtgcctgc aagacatatt ccatgttgcg
     5461 tgagcctgcc ccttttaatt ccaagaatac gcccatgtgt tcgtcatcag aagccatgac
     5521 cttgatttct ccgtaggcgt aatgctcttt atagccataa tacccatagt cataatggat
     5581 aaaataatct gctttcatgc caagcacctt tcggataatt tccaatgcgt cagtcgttgg
     5641 ataacggaca gacagataat caacgagcag ataaaaaggc ttatcgcaga tataataatt
     5701 cagacaacgc aggataatat tttgtgtttc cttgctggcg ttggcttttc cgttctcaaa
     5761 gcggttgata ctatatcttg atacatgggt aagacttgca agttctcctt gtgaaagacc
     5821 gcttgcaaga cgtttgttct tcatttcaag tgcaaattct aaattgttca ttgtattcct
     5881 ccaatcccac aatagccttg aaatacctgt aaacctctat tcaaaaagtg tgatatgcac
     5941 tatcttctta aaacttctca ttactactca caactttggg attgtatggt ttttgacttg
     6001 tcttttgtga aatagtgccc ccctgttaga taacgggggc acagacaagg ctcgcaggct
     6061 cgccagccga caaagcattt cttccccttg acacaaggtg tcatggcggt aagcctgaga
     6121 ggcttccgcc accgtgtcag aaatactttg tcagcaagtt ctgttttgcc ttttctaaca
     6181 agttccattt caaccgatat tcatatctac ggatttaatc agataggtat ttctttttga
     6241 atgtttttat atttcattct attgtgctta ttcgattaca agaacatagt aattcttata
     6301 tgcttatttg tcaatatctt tttttgcctt tacaaatcat attaagcatg atagacttat
     6361 aatagcgaag atataacgag atatggaggg ttatgcaatg aaaataagcc atttaggaaa
     6421 taatatacag accataagaa aatttagggg aatgaaacaa caggaacttg cggacaaaat
     6481 cggtatcaat atgcagagcc tttccaagat tgaaagaggg ttgaactatc ctgcctatga
     6541 aacgctggaa aagataatgg aagtgctgga cgttacgccc aatgaattgt tgtcgggaga
     6601 atggaagtat gtcaatcaat ctgaaaagga agtctgtcag tttttaagag ccgaagaacg
     6661 tctaaatgca gaactgaaac atggacacta tgataacttc tttgacagcg aggaagaatg
     6721 gctggaatat gagttagaaa agttacggga atatattaca gactatatca acgggaaaag
     6781 cattgaagcg tccgaccttt acccaatcaa agaatttatc cagcatttga agtttcaaaa
     6841 attattagac cgctatgatg atttatacag tatggatatg tttggggaaa gcatagaggg
     6901 acataagtat aagacacctt atcaagtcgt gaaaatgata aacccgaact caaaagaaga
     6961 tatggaactg ttacgagaag tattaaggaa taacaatttt gatgatgagg acgagtaact
     7021 tttttcctct tgcgagaaga tacagcaaac aaaaagcccg atcaagagtg caaagcacca
     7081 ccaatggtgg caaagctctt gacagggctt tttcttttct gttgtgggca atcaagagga
     7141 agaaagggat aaaatctgat ttgtggtaaa tcaggcaaag gcaacgcagg tatctcaatt
     7201 tgatttggta cttgaaaaga acgggagtaa ttggaagatt atagaataac aaatattggt
     7261 acattattac agctattttg taatcacgta ctctctttga taaaaaattg gagattcctt
     7321 tacaaatatg ctcttacgtg ctattattta agtatctatt taaaaggagt taataaatat
     7381 gcggcaaggt attcttaaat aaactgtcaa tttgatagtg ggaacaaata attggatgtc
     7441 cttttttagg agggcttagt tttttgtacc cagtttaaga atacctttat catgtgattc
     7501 taaagtatcc ggagaatatc tgtatgcttt gtatgcctat ggttatgcat aaaaatccca
     7561 gtgataaaag tatttatcac tgggattttt atgccctttt gggtttttga atggaggaaa
     7621 atcacatgaa aattattaat attggagttt tagctcatgt tgatgcagga aaaactacct
     7681 taacagaaag cttattatat aacagtggag cgattacaga attaggaagc gtggacaaag
     7741 gtacaacgag gacggataat acgcttttag aacgtcagag aggaattaca attcagacag
     7801 gaataacctc ttttcagtgg gaaaatacga aggtgaacat catagacacg ccaggacata
     7861 tggatttctt agcagaagta tatcgttcat tatcagtttt agatggggca attctactga
     7921 tttctgcaaa agatggcgta caagcacaaa ctcgtatatt atttcatgca cttaggaaaa
     7981 tggggattcc cacaatcttt tttatcaata agattgacca aaatggaatt gatttatcaa
     8041 cggtttatca ggatattaaa gagaaacttt ctgccgaaat tgtaatcaaa cagaaggtag
     8101 aactgtatcc taatatgtgt gtgacgaact ttaccgaatc tgaacaatgg gatacggtaa
     8161 tagagggaaa cgatgacctt ttagagaaat atatgtccgg taaatcatta gaagcattgg
     8221 aactcgaaca agaggaaagc ataagatttc agaattgttc tctgttccct ctttatcatg
     8281 gaagtgcaaa aagtaatata gggattgata accttataga agttattact aataaatttt
     8341 attcatcaac acatcgaggt ccgtctgaac tttgcggaaa tgttttcaaa attgaatata
     8401 caaaaaaaag acaacgtctt gcatatatac gcctttatag tggagtacta catttacgag
     8461 attcggttag agtatcagaa aaagaaaaaa taaaagttac agaaatgtat acttcaataa
     8521 atggtgaatt atgtaagatt gatagagctt attctggaga aattgttatt ttgcaaaatg
     8581 agtttttgaa gttaaatagt gttcttggag atacaaaact attgccacag agaaaaaaga
     8641 ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct gaacagagag
     8701 aaatgttgct tgatgccctt ttggaaatct cagatagtga tccgcttcta cgatattacg
     8761 tggattctac gacacatgaa attatacttt ctttcttagg gaaagtacaa atggaagtga
     8821 ttagtgcact gttgcaagaa aagtatcatg tggagataga actaaaagag cctacagtca
     8881 tttatatgga gagaccgtta aaaaatgcag aatataccat tcacatcgaa gtgccgccaa
     8941 atcctttctg ggcttccatt ggtttatctg tatcaccgct tccgttggga agtggaatgc
     9001 agtatgagag ctcggtttct cttggatact taaatcaatc gtttcaaaat gcagttatgg
     9061 aggggatacg ctatggctgt gaacaaggat tgtatggttg gaatgtgacg gactgtaaaa
     9121 tctgttttaa gtatggctta tactatagcc ctgttagtac cccagcagat tttcggatgc
     9181 ttgctcctat tgtattggaa caagtcttaa aaaaagctgg aacagaattg ttagagccat
     9241 atcttagttt taaaatttat gcgccacagg aatatctttc acgagcatac aacgatgctc
     9301 ctaaatattg tgcgaacatc gtagacactc aattgaaaaa taatgaggtc attcttagtg
     9361 gagaaatccc tgctcggtgt attcaagaat atcgtagtga tttaactttc tttacaaatg
     9421 gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt tactaccggt gaacctgttt
     9481 gccagccccg tcgtccaaat agtcggatag ataaagtacg atatatgttc aataaaataa
     9541 cttagtgtat tttatgttgt tatataaata tggtttcttg ttaaataaga tgaaatattt
     9601 tttaataaag atttgaatta aagtgtaaag gaggagatag ttattataaa ctacaagtgg
     9661 atattgtgtc ctgtatgtgg aaataaaaca cgattaaaga taagggaaga tactgaatta
     9721 aaaaaattcc ccctctattg tccgaaatgc agacaagaaa atttaattga aataaagcag
     9781 ttcaaagtaa ctgtgattac agagccagac gcaaagacgc agagccgata aaatgagatt
     9841 aatacaatct cattttatcg gctctttccg ttatgtatgg attcttttaa ttagtcttcg
     9901 atgtttcttg cttcgttgat accgctggct aaagattcca ttaaggatag ttctttgtct
     9961 gtaaagctat ccatgtattt ctctatctgt aatcgtcggg tgctttttac caagttatta
    10021 gcaggtaaga aaaattcatc aacggaaaca tgaagtaacg atacaaggtc ataaagaact
    10081 tgtatgctgg ggtgttgccc tttattttca atattagtta agtaccgtgg gtcaatttca
    10141 atcaatgctc ccacttgttc acgagttaaa cctcgtttca atcgagcttc tttaatggct
    10201 aaaccaaagg ctctaaaatc atatttatct tcttttttac gcatagtaga ccacctctat
    10261 acattttatt gttcctactg aattaaaaac aggtatagaa aaacgtgtta tatggtttat
    10321 aggtttatat ttaataaaaa gcactactaa acgccaataa aaaaaaccgt tatatggtag
    10381 tgctatttac gctgttaaaa tattgtatat tacttccaaa tggcggtttg ttggaggtca
    10441 acgtcgccat gaagtacatc atatacaata aatttcctta cattgggttc ttgtcaaaaa
    10501 aagtcgtcta tctgcaatag ataagtacgt ccaccaatgt ggttttataa atcatataga
    10561 tagaataaca gaagcatgta aacagagaaa taaatctgtt tatatgcttt tttggctatt
    10621 cagaactttt ttacaaagtt tatttatcag taatgcaaca aatccccctt tcacattggg
    10681 actaagagtg aaaggagata aacgagcaag gctcacttcc tttcctagac agaaaggggg
    10741 tgagattatg aggacatctt ctttcgagca tatcgtcaga ttacagttca attctttaat
    10801 gctgattgtc attaagaaca aagtgaaaag tcgaaacaga cagcttgcaa gacattctaa
    10861 acgtgaagta ttgttttgtg aaatagccga aacgaaacaa gttgaacgtg gaacgattga
    10921 tacctgtttt tatgattgtg tatcatttaa agtcttgcac tttacaattt atgtatctga
    10981 tgaaacactt ggtacagttt tacatagact atcagaaaaa cagcgtagtg cgattttatt
    11041 acgttatttt caaggaatga acgacagaga aatttcagaa ctgtaccatg tatcacgttc
    11101 tgctatttgc agtagaagaa ctagaggact aaggaagtta aaaatgcttt tgagcgaaag
    11161 gaagtaaaga ttatggctat ccctcttttg aattgatttg caaagcgtca agcggagatg
    11221 aaatggcagt taaagagatt ttgaagtttt atgacggtta tatttctaaa ttatgcttgc
    11281 gtccttttta ccactctgaa agtggtaaaa tcattatgca ggttgatgaa gaactaaaag
    11341 gagaaattca tacagacatg atgaaagcaa tattgaaatt tgaaatcgga gtgaagtaat
    11401 caactgtata aaaaaatcgg gagatacaca gtttcgtgtt atctccctta gtagttttac
    11461 ggtcatatca aggctcattc aatcgtggta aattcgtggt aagatgagtg tttttctata
    11521 cttcaaaatg cttgaatgta tagtgtttat gcggataatt gaaaattaga tataaaattt
    11581 aataaaaacc aaaaatgcac ggtcgccatg aagggaccgt gtatttttat cttcttattt
    11641 tctcacctaa tcgtatcagc cgcatcgctt gttttcctgc acaaacaagc aatcgctcta
    11701 cctgttccat cgcataagaa agtaccatcg gttcattgat gatatcaagt acatagtcga
    11761 taccaccctc acttaattga gcttgatttt tcttctccat attatgtagt tgtgggacaa
    11821 ctttctgaca atcagcaatt gtgtcaagaa cggatcaatc ctttattgga gttgctcgaa
    11881 agggtcttct cttattacgg acaggctttg ctcactgtac atagacaaca aatcattatc
    11941 ctagtcaatc ggatttccag agcctcattg ctatcattgc ttgataaaat ccagacaaag
    12001 tttaataaaa tgtatcagtt acagctaaac tttggaattg ggtcgctttg ttatacagag
    12061 caagagacac cgcaatcttt ccttcatgcc aaacaagttt gcgaatggat agctttccat
    12121 caatctgtga acgagatccg cttttttgaa gatttagacc tcgggatcgt tttaccagca
    12181 atcccgtcag atcaacgaac attatatgta aaacgtattt tgaaaagcct gacagaagaa
    12241 gaagtacatt tgttcaagaa gactttggca tgcttcagta aaaataacgg aagcatcaaa
    12301 aattgctcag aagaattatt tattcataaa aacaccttgc aatatcgatt gaacaagttc
    12361 cattctctca ctggttatac ccctagaaac tatgacgatt atcatattct aaagctggct
    12421 tttttgttgg tacaaacatg acaactacaa aaaaaccagg cgaacatatg attgttcgtc
    12481 tggtttttaa ttattaatga cttagcgttt ttactagaaa aactacttcc gctttttccc
    12541 gccaaaagca tctttaaact tatcgaagaa accgtcttct tgctgttctt caactttgat
    12601 gccgccagct tcagcgaatg cacgtaatgc ttctctttgt tgttcattta gatttttcgg
    12661 tgtgatcagt tttactttga catgttggtc gcctgttgca ccaccacgta acttcggagc
    12721 gccttttcca cgtaaacgga agttcgtacc tgtctgtgtt ccagcaggga tcttcaattt
    12781 cacatctcca tggacagttg gtacttggac ttcatccccc aaagctgctt gtacaaaact
    12841 taaaggcaat tcatagtaaa tttcagaacc ttcacgatca aagatatcac tttcttctac
    12901 acggaagacc acatacaaat caccaaatgg tccgccgttt tctccagctt ctccttgacc
    12961 agctaaacgc atttgttgtc cttcttctac accagctggt acattcactt taactgaatg
    13021 tgctttcttc tcatgtcctg agccatgaca ggttggacaa ggttctttga tttctttccc
    13081 tgtcccatga caaacatcac aagtttgacg gctcatcact cgtccaagtg gtgtttggcg
    13141 ttctacatta atcgtacctg caccatgaca tttatgacaa gtagttggtt gtgtgcctgg
    13201 ttttgcaccg ttcccaccac aagtatgaca gatttcttcc cgattatact tgatttcttt
    13261 ttctacacca aaaatcgctt cttcaaaaga caagtcgacc gtatattgca aatcagcacc
    13321 ttgacgaggc gcatttggat cgactgaccg accgcctcct ccaaagaaag agtcgaagat
    13381 atcttcaaaa ccaccgaagc cgccaccaga gaagccgccg aatccaccaa atccgccacc
    13441 agctccacca gcaccgtaat ttggatctgt gcctgcatgc ccgtattggt catatgcagc
    13501 gcgtttttgc gggtcactta aaatctcata tgcttctgat acttctttga atttttcttc
    13561 cgcatcaggt tctttattga tgtcaggatg gtattttttc gaaagcttac gataagcttt
    13621 ctttatttca tcttctgatg ctccttttga gagccctaag acttcataat aatcacgttt
    13681 tgttgccata ggctttcctc ctattggttc tcatcatttt cacgtcgctt gttatcatat
    13741 catattttgt ttgaaacaaa aaccgatact tcaaactatt tttcaaaaga aaaagccaaa
    13801 gtagcaactt tggccttctc tcgaaattca gtgaactgat tatttatcgt cgccatcgac
    13861 ttcttcaaaa tcagcatcaa cgacatcatc tgctccgcct tgagctgctt ctgggttttc
    13921 ttgagcttgt tgttgtgccg cttgttcgta tagtttaaca gtcaagtttt gaacgatttc
    13981 gttcaatgca tcgcgtttag ttttcatttc ttcgatgttg tttgcttcaa cagcggcttt
    14041 caattcatcg cgagctgttt ctgctttttt cacttcttct tcgtccactt tgccttctaa
    14101 ttctttcaat gttttgtcca cagagaatag caatgcatct acatcattac gtaggtcaac
    14161 ttcctcttta cgagctttat ctgcttctgc atttgcttct gcatctttta ccatacgttc
    14221 gatttcttcg tctgttagac ctgaagaaga tttgatcgtg attttttgtt ctttttgtgt
    14281 acctagatct ttcgcagata catttacaat cccgttttta tcaatatcga atgttacttc
    14341 gatttgtgga ataccacgag gagctgctgg aatatctgtc aattggaatc ttcctagagt
    14401 tttgttgtca gcagccattg ggcgttcacc ttgtaataca tggatgtcta ctgctggttg
    14461 attatctgcc gcagttgaga atacttgtga tttacttgtt ggaatcgttg tattgcggtc
    14521 aatcaattta gtgaacactc cacccattgt ttcgatacct aatgacaatg gtgtaacgtc
    14581 aagtaaaaca acatctttca catcaccagt gatcacgcca ccttggatag cagcacccat
    14641 agcaactact tcatcagggt taactgattt gtttggttct tttcctgttt ctttacgtac
    14701 tgcttctaca actgcaggga tacgagtaga tccaccgact aaaataactt cgtcgatttc
    14761 tgattgtgat aaacctgcat cttttagcgc ttgacgtact ggtacttttg tacgttctac
    14821 taagtcagct gttaattcat caaattttgc acgtgtcaat gtcatttcca aatgcaatgg
    14881 tccagattct cctgctgtga taaatggcaa gctgatttgt gtgcttgata cgccagataa
    14941 atcttttttc gctttttcag cagcgtcttt caagcgttgt aaagccattt tgtcttgaga
    15001 caagtcaatg ctgttttctt ttttgaattc tgctaccatg tagtcgatga ttttgttatc
    15061 aaagtcgtcc ccacctagat ggttgtcgcc agctgttgac aatacatcaa agacaccgtc
    15121 tcctagttca aggatagata cgtcaaaagt accgccacca aggtcgaata ctaagatttt
    15181 ttcgtcgcga tctgttttat ctaaaccata ggcaagagca gctgcagttg gttcattgac
    15241 gatacgttct acttctagac cagcgatctt accagcatct tttgttgctt gacgttgtgc
    15301 atcattaaag taagctggca cagtgataac tgctttttcg actttttcac ctaaatagtc
    15361 ttctgcgaat cctttgatat attgaaggat cattgctgag atttcttgtg gtgtatatga
    15421 tttaccttct acatctactt tgtaacctgc ttcaccgata tggcgtttga ttgatgaaat
    15481 cgtatttggg tttgtcacag cttgacgttt tgcaacttcc cctacttgga tttctccatt
    15541 tttgaatgat acaacagaag gtgttgtacg gtttccttct gggttagcga tgatttttgc
    15601 ttcgtttcct tccaatactg ctactgcaga gttagtcgta cctaagtcaa taccgataat
    15661 tttactcata attaacgtct ccttctttaa ttgaaagtgt aagttttata tccgcacaat
    15721 tattgtgcaa caatgaccat ggttggtctt aacacgcgat catgtaattt gtagccttct
    15781 tgtagtactt ctacaatcgt atcggctggt gtatcttcag tcgctggaac agtttgtact
    15841 gcttgatgaa gattaggatc aaaggcttct cctttagcag ggattttttc aattccttct
    15901 tcttccagtg cgttacgcaa actttccaag accatttcca cgcctttttt caaacctgcc
    15961 ccttgatcat ctgacacttc tgtggctaat gcacgttcca ggttgtcaat tgatggaagt
    16021 aattttttgg ctaaatcttg tgaacggtat ttttgtaatt gctcacgctc attttttcct
    16081 cgatttgcca tatttgcgat ttcagcacga gcccgcaagt atttatcttc catttcttct
    16141 aactcagctt ttaatttcgc tgtttcgaat tcttccgctt cagcttctga aatacccgaa
    16201 tcttctgcag cttcgacatc gatttccggt tctggctgag catctgtcat ttctttatct
    16261 agttcttctt gattttcttt cacgatacag tcttcctttc attattcagt cagctgtttg
    16321 attgctctta ctaagaaaga gagaacgatc agctgcaaat agcttcgtta ttcacattac
    16381 gagtttgaca agtcaagcga acggtagtaa tcagcaagtt tagctgctag ttctcttcga
    16441 taaacatcga ctagaccgaa catccgagaa taaggcatac tagtaggccc taacaaggct
    16501 attgtacctc ttccatgacc atctatctcg taacttgctt gaatcatact catattctgc
    16561 aataaatcat tgcctaattc agcaccaatc ttgatatgaa tattgctatc catcggagtg
    16621 atcatttgtg taagtccgtc tgagtctttc ataaaagaaa acatcgattt gaactgatca
    16681 agatcttgac ggggctcata atccaagaga ttcatttgtc cactgacata aaccttatct
    16741 tcaaaaacat gaccaagaac ggtgtcaaat aactgagaca tcccttctgt cgtttggaaa
    16801 tatttatgaa gaatcatcgg aatctccgta cgaagtttat ggtacaccgt catcagagga
    16861 tctccgacta gacggtcgtt tacaatctgt accatttttt ctagttcctg acttcccata
    16921 tattcgggaa gagtgaacac ttggctttct acattcccca tatctgttac gacgatagca
    16981 ataatctgtc gattattcaa gggtacgata cgaaagccag tcagtcggcg gtcttttatt
    17041 tcaggtccca atgaaaaagt cgtatagctt gtcagctcag ataatatctc agcagattgc
    17101 ttaataatct cattgatttc atgaaattct ctgcctagag actgacgaat cagcagcaaa
    17161 tcatcttctg ctacgcgagc aggttttaac aaatgatcca cgtaataacg atagcctaaa
    17221 gcagatggaa tccgcccaga agaagagtga gtcttcaaca gcaatccttc atcttctaat
    17281 gctttcatat cattgcgaat cgttgctgag ctggccttga ctccctcaga catcaatgtt
    17341 ttggaaccaa cgggcacgcc actactcgta tagtgttgga tgatcagacg taaaatgtcg
    17401 ttttgtcgct ttgttaacat cttcctcacc tctcattagc actcatatat tctaagtgct
    17461 aatccactta taaatatagc aagaagccct tctcctgtca agagaaagac tccatttttt
    17521 agcactctaa acacaagagt gctaattcta aataattgat gtaaatccag ttatatcaac
    17581 gtttcaatag tcctttattg tatttttgaa ctttttttat ttaaattcag ataaaaaaca
    17641 aatattcttt aaactatttt tcaaacaaaa agcgctcaaa cacattgttc cccaaaaagg
    17701 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    17761 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    17821 gttttttagt aatccctgtt tttttcttag tcctaagaac atttcttctt ctatttgatt
    17881 ttttcgtgtc aatttttctt cttccaatac cgggcgttca tttgctcgta atgggcgtaa
    17941 atagtgttgg attggtcctt tgttgcggta acgaatatct ccaagataac cgctagcacc
    18001 agctccaaac ccaaaatagt ggtcattgtt ccagtacatg aggttatgtt gagattcatg
    18061 tccggagagc ccaaagttac ttatttcata ttggtgacgt ccatgctttt ccattgcttc
    18121 aatcgtctct tcaaacattc tggtttccgt ttcctgatca ggcagttcga gtcttccttg
    18181 tcgtacccag ttcatgaaca ttgttttgtt ttcgaggatc aatgagtaca aagaatagtg
    18241 aggcagatcc aaagccaaag ctttttccaa agtatccaga tatccttcta gtgtttgtcc
    18301 cggtaatgca taaatcaaat cgatgcttac attggtaaaa ttttctgctt ctaaaaattg
    18361 catagtttga tacacatctt ctgcggtgtg tttacgtcca atttttttca ataagcgatt
    18421 atcaaatgtc tgcactccca tggataaacg attgacgcca tagtttttca ttacttgaag
    18481 tttttcacgg gtcaaatcac ctggattcgc ttctacagta aactcattac cattttgaaa
    18541 aggcaggatc tctctagcac ctgataacag tcgatcaagt tgtttagcag ataaagaagt
    18601 tggtgtcccg ccccctacgt aaagtgtttc taaattcgct gtcggatgac tttctagcat
    18661 tattttcatt tcattcagca gcatttccac atactcatct actggctgcc cttctaaaaa
    18721 aactttatta aaatcacagt aatagcagat atgctcgcaa aatgggatat gaatgtacgc
    18781 agaaaccttg ttttctgcgg gatttattgt ttctatcatt gaaattcttt ctccttacgt
    18841 cgaaaagtcg tgcctgtttt cgaccttctt aaaatcgcgt tcaagcacca actcattgaa
    18901 ataagccgca tatttagaat caaggaaacc tagctctaaa aatgcttgct tattgtcatc
    18961 ggagctacac ggtgctttta cagcctctcg ataaacggta atcgcaaagc tgaccttcgg
    19021 cattaagtca aaatctgcaa aaatatgaaa agctattttc acagattcct ccttaagtgc
    19081 tcagaccaca acgctttgct tacaacctct catatacgca tactaaacgt atttggcgaa
    19141 caaatccact aaaaaatcca agaatcgact tcttggatga ttttttcttt cattaaactg
    19201 gttacctagt tatatatctc tagggttgtt ttcatgtctg agtgcccgat atgatttatt
    19261 tttcgtttct ataattactt tttgccttcc agtttctttt aatattgtac tcaagcaagt
    19321 gggaattggt tcttttgtca tccatgtatg caaaatgtta taatgctggt aaacaaaaat
    19381 gattacctgt catttttatt tagttcgtat ttaattaagt tgactatctg tatactctgt
    19441 ctaaaactta agaattgttg ttttcgctgt cgctacccaa ttttaatgaa tgtctgtagg
    19501 ttaaaactgt tgtccttgtt atagatagta aaggagagct tgaaaatgaa aactgcgatt
    19561 acgtttctat tagaggaata tgccgattgg gaaggtgctt atattactag cacactaaac
    19621 caaaatgaga aatggcaaaa caaaactgct tctatccaat cagaggtcac ttccattgga
    19681 ggattaaaaa caaaaatcga ttacttaatc gaacaaatac ctgaaaaagt tgaccttttt
    19741 attatgatcg gtggcaattc atgggacata aaaaatcatc atttatatca aataatcgat
    19801 aaattgttaa aaacagacgt accagtagct gctatttgtg gggcagttga ttatttagct
    19861 cagagcggat tattgcaagg ttacaaacac accggaaatg cgcaatttcc ttggttagaa
    19921 tcggaaaatt atcagaataa agaagatttt atagaacaac aagctgtgaa agatcgtaat
    19981 ttgattactg cgaatggaac agctcctata gaattcacag acttgattct tcaagcagtc
    20041 cattttgaaa cagaagaaat aagagaaaaa acgattcgtc tatacaaact tggattctat
    20101 gattactata aaaagtacgg agatcctttc tcctaatcat tctcttcaat taaatactcc
    20161 ttatcaatag tgtgacaagt aattttactg atgattattt gtcacacttt ttctgacatt
    20221 tatcaaagaa gatacactct atttatattc atagacaagg ttttacggat tcaaaaattc
    20281 aggattatgc tatactggta ttaaaaaaat aatgaggtat tttatatgaa aaagttaggt
    20341 tttattttat tgttggtctc tttattcctt gtaggatgta cgtctcctag taaagaaaat
    20401 agtgatccat ctgcgaccac agacaaaagt tcaatcagac aaaccagttc agctgaaaaa
    20461 tcaagtactc agaaatcagc ttctactgaa acgaatacat cttcaacaag cagtaaagtc
    20521 tcagagccca atacttcttc tacatctagt agtacagttg agacaaatat ttcttcttca
    20581 acaatagaca caacctcctc acagtcttct cagtcaatag atccgttagc aggatattct
    20641 gatctacaaa ttgagtacgc acgtgtttgg ctagctatta gaggtaatac ttttagagat
    20701 acatcgggtg atttcgaatt acatgttatt cattatcctg caggaacacc tatcgcccct
    20761 tatgatgcag ggagtgcagt ctatccgaaa gatgtggtta tgctcactgg gaaatacagt
    20821 taccagggag ttttagtgta ttcaagtaac catgacggga caatcactag ttatccagtt
    20881 ccttcacatt ggcaaatgcc tgcagatcaa acatcttatc cagcgttcat tagagaaaca
    20941 actcaagaaa tcattgataa tgcatctgta gtagctattc ctacaggaaa tcccaatgac
    21001 gtgaaacagt taatcgatgt gacagttata gataattaga ctttctaata tgttatacaa
    21061 aaagaagctg acagcatttt gtggctctat aatttatggg actgatgaat cagaat
//
LOCUS       DAZKOU010000296         8014 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.guided.108, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000296 DAZKOU010000000
VERSION     DAZKOU010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.guided.108"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG827_003065"
     CDS             complement(134..577)
                     /locus_tag="ACG827_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0485090.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG827_003066"
     CDS             complement(795..2135)
                     /locus_tag="ACG827_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0485091.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG827_003067"
     CDS             2404..3300
                     /locus_tag="ACG827_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0485092.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG827_003068"
     CDS             complement(3450..4391)
                     /locus_tag="ACG827_003068"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0485093.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG827_003069"
     CDS             4517..5638
                     /locus_tag="ACG827_003069"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0485094.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG827_003070"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG827_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0485095.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG827_003071"
     CDS             7465..7992
                     /locus_tag="ACG827_003071"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0485096.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2660 a         1497 c         1588 g         2269 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaa
//
LOCUS       DAZKOU010000297         7111 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.guided.114, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000297 DAZKOU010000000
VERSION     DAZKOU010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.guided.114"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..794
                     /locus_tag="ACG827_003072"
     CDS             147..794
                     /locus_tag="ACG827_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0485097.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(1487..2425)
                     /locus_tag="ACG827_003073"
                     /pseudo
     CDS             complement(1487..2425)
                     /locus_tag="ACG827_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(2425..3042)
                     /locus_tag="ACG827_003074"
     CDS             complement(2425..3042)
                     /locus_tag="ACG827_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0485098.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(3056..3226)
                     /locus_tag="ACG827_003075"
     CDS             complement(3056..3226)
                     /locus_tag="ACG827_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485099.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(3574..5064)
                     /locus_tag="ACG827_003076"
     CDS             complement(3574..5064)
                     /locus_tag="ACG827_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0485100.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(5107..5406)
                     /locus_tag="ACG827_003077"
     CDS             complement(5107..5406)
                     /locus_tag="ACG827_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485101.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(5772..6056)
                     /locus_tag="ACG827_003078"
     CDS             complement(5772..6056)
                     /locus_tag="ACG827_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0485102.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(6575..7060)
                     /gene="vanZ-A"
                     /locus_tag="ACG827_003079"
     CDS             complement(6575..7060)
                     /gene="vanZ-A"
                     /locus_tag="ACG827_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0485103.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT         2194 a         1223 c         1068 g         2626 t
ORIGIN      
        1 ttaggttttg gaagtaaaat aagttttatt tgataaaaat tgctaattca gtataattaa
       61 tatttacgag gtgacataac gtatgaaaaa atcagaggat tattcctcct aaatataaaa
      121 atttaaaatt taggaggaag ttatatatga cttttaatat tattgaatta gaaaattggg
      181 atagaaaaga atattttgaa cactatttta atcagcaaac tacttatagc attactaaag
      241 aaattgatat tactttgttt aaagatatga taaaaaagaa aggatatgaa atttatccct
      301 ctttaattta tgcaattatg gaagttgtaa ataaaaataa agtgtttaga acaggaatta
      361 atagtgagaa taaattaggt tattgggata agttaaatcc tttgtataca gtttttaata
      421 agcaaactga aaaatttact aacatttgga ctgaatctga taaaaacttc atttcttttt
      481 ataataatta taaaaatgac ttgcttgaat ataaagataa agaagaaatg tttcctaaaa
      541 aaccgatacc tgaaaacacc ataccgattt caatgattcc ttggattgat tttagttcat
      601 ttaatttaaa tattggtaac aatagcagct ttttattgcc tattattacg ataggtaaat
      661 tttatagtga gaataataaa atttatatac cagttgctct gcaacttcat cattctgtat
      721 gtgatggtta ccatgcttca ctatttatga atgaatttca agatataatt catagggtag
      781 atgattggat ttagttttta gattttgaaa gtgaatttaa ttttatacac gtaagtgatc
      841 ataaaattta tgaacgtata acaaccacat tttttggttg cttgtggttt tgattttgaa
      901 tttggttttg aacttatgga ctgatttatt cagtccattt tttgtgcttg cacaaaaact
      961 agcctcgcag agcacacgca ttaatgactt atgaaacgta gtaaataagt ctagtgtgtt
     1021 atactttact tggaagatgc accgaataaa aaatattgaa gaacaactag caaaagattt
     1081 taaagagtta ttttatttta agtctttata acatgagtga agcgaatttt taaatttcga
     1141 tagaaatttt tacatcaaaa agccccctgt caaaattgac gaagggggtt ttttggcgca
     1201 cgcttttcgt tagaaatata caagattgaa aatcgtgtat aagtgcgccc tttgttttga
     1261 acttagcacg ttacatcaat tttttaaaat gatgtataag tgcgcccttt taaattttga
     1321 gtgattatat tttttgagtt agaaaaaggg attgggaaaa tttcccaaaa taatttaaaa
     1381 aataagcaaa aattttcgat agagaatgtg ctattttttg tcaaaggtgt ataccttgac
     1441 tgtgcttgct gttacattaa gtttattttt aagttattaa aaaagaaata gcttttaaag
     1501 tttggctcgc tgtcgcttta taaagctgat tgacttttga ttgcaaacta cttaaagaaa
     1561 acaaactcgg actattcgtt ttcttctctt tggtttgaac atcagcaatt atcccctctt
     1621 gattgcctat tttagcttgt ttagaagaaa caaaagctaa aagctcctct tgggttttaa
     1681 aacgctgtgt ggggcttaga acgcccttaa acgacccttg gtttactttt atactagctt
     1741 ccacctcgaa aaaaggttct tttttaaaat tctctatggc ttcctggcgc tgaaaaataa
     1801 ggtataaggt gggcgtttga acacgtccta gtgaaaatgt accttgtacg cccttctgtt
     1861 gtaaatttaa cgtatacaaa gggcttgcgt tcatgccgat caaccaatcg gcaatttggc
     1921 gtgtttgcgc ttcttgataa aagggatagt aattcattcc aggttgcaaa ttttgaaaac
     1981 cgcttcggat tacatctttt tctaagctat tgatccatag tcttttaaat gttttatctt
     2041 ttgaaaaggc atttgcttta tggataatcg accaggcgat attttcacct tctctgtcgc
     2101 tatctgttgc aacaataatt gtatttgcct ttttgagaag ttctgcaaca attttaaact
     2161 gctttccctt atcttttgca acttcaaaat cgtatcgatc aggaaaaatc ggcaaagatt
     2221 caagtttcca attttgccac ttttcgtcat aatgacctgg ttctgctaat tccactaaat
     2281 gcccaaaacc aaaggtgata aacgtttcat ctgtaaatag tgggtctttg atctcaaaat
     2341 aaccgtcttt tttggtgctt tgttttaaag cacttgcgta ggctaatgcc tggcttggtt
     2401 tttcagctaa aataaccgta ctcattaact atccctcttt tcattgtttt ttctttgatc
     2461 gactgtcacg ttatatcttg ctcgatacct tctaaacgtt cggcgattga ttccagtttg
     2521 ttcttcaact tctttatcgg ataaaccatt caaaaacaaa tcgaaagcat gctttaaacg
     2581 tggatcattt tctttaaatt tatgctgacg acctttgaat ttgccttttt tcttagcaat
     2641 ttcgattcct tgtgcctgac gttccttaat tttttttcgt tctgattctg cttgatactt
     2701 gtacaattca atgacaaggc tattaatcaa acgccttaaa ttttcatctt caataccatt
     2761 cattgagggt aaatttaaga cttccagggt tgccccctta atttgaattt gattcatcaa
     2821 ttctgttaat tctttattat ttcgtcctaa tcgatctaat tcagtaacaa taacaatatc
     2881 cccttcacga atatagttaa gcatagcttg taattgtggg cgttcgaccg attgaccgct
     2941 taatttgtct gaaaagacct tagaaacgcc ctgtaacgct tgtaattgcc gatctaagtt
     3001 ctgttctttg ctactgacac gtgcataacc aattttagcc attttcaacc aacctctaaa
     3061 attctctcgg ttgcaataac caatcagcaa tatctacttt ttcaatttca aattgcttat
     3121 cagaaattgt cttttcgtaa gcgataaaat cttgcgcata ttgttgctca ttaaaaatag
     3181 ccaccacttc gtcattttct aaaactcgat aaataaattt tttcatttta ctcctcctat
     3241 tatgcccaac ttaaatgacc tattcaccaa gtcaattata ctgctaaaat catattagga
     3301 caaataggta tactctaatg acctataaaa gatagaaaat taaaaaatca agtgttcgct
     3361 tcgctctcac tgcccctcga cgttttagta gcctttccct cacttcgttc agtccaagcc
     3421 aactaaaagt tttcgggcta ctctctcctt ctccccctaa taattaatta aaatcttact
     3481 ctgtatattt ctgctaatca ttcgctaaac agcaaagaaa aaacaaacac gtatcataga
     3541 tataaatgta atggcatagt gcgggtttta ttttcagcct gtatcatagc taaacaaatc
     3601 gagttgtgtg tccgttttag ggcgttctgc tagcttgttt aaagtctctt gaatgaatgt
     3661 atgctctaag tcaaaagaat ttgtcagcgc ctttatatag ctttcttttt cttctttttt
     3721 tactttaatg atcgatagca acaatgattt aacactagca agttgaatgc caccatttct
     3781 tcctggttta atcttaaaga aaatttcctg attcgccttc agtaccttca gcaatttatc
     3841 taatgtccgt tcaggaatgc ctagcacttc tctaatctct tttttggtcg tcactaaata
     3901 aggcttgtat acatcgcttt tttcgctaat ataagccatt aaatcttctt tccattctga
     3961 caaatgaaca cgttgacgtt cgcttctttt tttcttgaat ttaaaccacc cttgacggac
     4021 aaataaatct ttactggtta aatcacttga tacccaagct ttgcaaagaa tggtaatgta
     4081 ttccctatta gccccttgat agttttctga ataggcactt ctaacaattt tgattacttc
     4141 tttttcttct aagggttgat ctaatcgatt attaaactca aacatattat attcgcacgt
     4201 ttcgattgaa tagcctgaac taaagtaggc taaagagagg gtaaacatga cgttattacg
     4261 ccctattaaa cccttttctc ctgaaaattt cgtttcgtgc aataagagat taaaccaggg
     4321 ttcatctact tgttttttgc cttctgtacc gcttaaaacc gttagacttg aacgagtaaa
     4381 gcccttatta tctgtttgtt tgaaagacca atcttgccat tctttgaaag aataacggta
     4441 attaggatca aaaaattcta cattgtccgt tcttggtatg cgagcaatac caaaatgatt
     4501 acacgttaga tcaactggca aagactttcc aaaatattct cggatatttt gcgaaattat
     4561 tttggctgct ttgacagatt taaattctga ttttgaagtc acatagactg gcgtttctaa
     4621 aacaaaatat gcttgataac ctttatcaga tttgataatc atagtaggca taaaacctaa
     4681 atcaatagcg gttgttaaaa tatcgcttgc tgaaatagtt tcttttgccg tgtgaatatc
     4741 aaaatcaata aagaaggtat tgatttgtct taaattgttt tcagaatgtc ctttcgtgta
     4801 tgaacggttt tcgtctgcat acgttccata acgataaacg ttgggtgtcc aatgtgtaaa
     4861 tgtatcttga ttttcttgaa tcgcttcctc ggaagtcaga acaacaccac gaccgccaat
     4921 catgcttgat tttgagcgat acgcaaaaat agcccctttg cttttacctg gcttggtagt
     4981 gattgagcga attttactat ttttaaattt gtactttaac aagccgtcat gaagcacagt
     5041 ttctacaaca aaagggatat tcattcagct gttctccttt cctataaatc ctataaaata
     5101 ggttgtttaa ttaacttggt ttgctttttc attcaactgt ttcaatattg catgttttga
     5161 aaaagatttt tttcctttat aagtcaattt ttttccacta atcgaataaa ttattttgtt
     5221 attttctatt aacttatata tataatcttc cccctccgaa gaaaaatact tatctgattt
     5281 tgtttctaag tagatatttc tcttttctaa ctctttctta aacgtttcta gtgtatagat
     5341 atttgctaat tttcttatct ccaataaact attttttata taagttttac attcatcatg
     5401 attcatacaa actccacctt ctataaatga atacaaaaaa agcaatcaaa cgatttccga
     5461 ttgattgctt aacaattctt aaattcagta gcttagatac ttgaaaactc tctgatttcc
     5521 ctatataatg atagtacggt tatataccgt cttcaaacaa agttaattaa ataacttctt
     5581 acgagggaag agttcatctg actaactgat aagcgttggt ttggcaatct tatcgggcta
     5641 tgcatttata aaatgtcgtc aaacatttta taaatgtgtc atggctcttt tttcgtttct
     5701 attcagttcg ttgtttcgtt atatctagta taccgctttt aaaaaaaata agcaacgatt
     5761 tcgtgcatta ttcacacgaa gtcattgctt ttttcttctt ccatttctaa atccaatgtt
     5821 acttgttctg attctgtttc tggttctggt tctgttggct catttgggat taaatccact
     5881 actagcgttg agttagttaa ctttgcaatt tgttctagtg tttttatggt tggatctgat
     5941 tttcctgatt ctattcgtga ataatttgat ctactcattt ctaattcttg ggagaatttt
     6001 tcttttgatt tggtacctct catctttttt aagctttctc taaatgctag ttccaagttt
     6061 ctcccccctc tttttgtgta ataaaattca cgctttattt aaattatagc ttttaatatc
     6121 ttaataaaca agtgattaat cacttgtgat ttgtgattat tccaaaagta ggtaatttca
     6181 ccaacctttt tcaaaagttt ttatcgacga ttattggact ggatcgtgtt tcctaaagcg
     6241 attctaagcg catgtttaat tttaaagtgg gtaattctct ttaaaacgtc ctacaaagat
     6301 tcaaaaaaag catggctgct agtgggggta ccgtcaggaa aatgcggatt tacaacgtta
     6361 aggaaattta ttaaagagac caattcctca gcactaaaat tgataattgg gctttctttg
     6421 ttactatagt gttaggtaag ctttttactt tatcaccggt tttcccctca cttcacacct
     6481 acgggcgagt ttccccacat cccgttttcc atctaatcca attcattcaa tatctttatg
     6541 tgtatgaaag atcagattgc ttgatttaga catcttacac gtaatttatt cttaaatggg
     6601 tacggtaaac gagcaataat acgagcaaaa gtatacctac aaaaataata actctgtcta
     6661 attttttttg attcatatgc ttattgctta aaccatataa tttcagtcca agaaagcctc
     6721 caacagtatt tgtaattaca tctgttatgt ctgtcgctcc aatagcgaag ataaattgaa
     6781 ttatttcaaa agtaagactt aaaaccagta caaaagcaaa cttaggtaaa aatccgattt
     6841 ctttaaaatt gacattcaaa agcaagccaa atggaataaa gattataaca ttatctatca
     6901 tctctctgaa attcccagta gcagtaaatg gagtcaagtt aagacttctt tgatgataat
     6961 taaatactga taaaatattg tattgtaatt tgaataacac taaccagatt agtgtcacta
     7021 aatataaagc tagcaatcct ctagataata tttttcccaa tataatttcc tctaattctt
     7081 ttgtttaagt tgtatcatta gtatagcaat g
//
LOCUS       DAZKOU010000298         5547 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.guided.125, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000298 DAZKOU010000000
VERSION     DAZKOU010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.guided.125"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..530
                     /locus_tag="ACG827_003080"
     CDS             66..530
                     /locus_tag="ACG827_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0485104.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            514..1143
                     /locus_tag="ACG827_003081"
     CDS             514..1143
                     /locus_tag="ACG827_003081"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0485105.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1497..2975
                     /gene="msr(C)"
                     /locus_tag="ACG827_003082"
     CDS             1497..2975
                     /gene="msr(C)"
                     /locus_tag="ACG827_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0485106.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3022..3468
                     /locus_tag="ACG827_003083"
     CDS             3022..3468
                     /locus_tag="ACG827_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0485107.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3667..4182)
                     /locus_tag="ACG827_003084"
     CDS             complement(3667..4182)
                     /locus_tag="ACG827_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0485108.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4299..5501
                     /locus_tag="ACG827_003085"
     CDS             4299..5501
                     /locus_tag="ACG827_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0485109.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1732 a          926 c         1191 g         1698 t
ORIGIN      
        1 tcttttttta ttctattatt gacaagtcaa tcgttgacga gtcaattatt agaaaggagg
       61 aaattatgtc tttagaagag attagtcacc ttttatatca aatcaaatta gccgatcaaa
      121 aaatcgtcca gatctttgaa gaaaatatcg gtataagtct tactagatac gagatcatga
      181 tgagactgaa agaagaaaat ccattgctac aaagcagttt gcaagaaaag ttgcagattg
      241 atcaaggagc agttactcgt catctgaagg tattggaagc aaatggctat gtttcgcgtc
      301 agagaaatcc tcagaacaac cgggaagtgt atgtagcgct aacagaaaaa gcccagaaga
      361 aaatcaagca ttgtgaaatg gaccataaag atacaggttc cgtgctatcc ccggctttca
      421 gcgatgtaga aatgaaacaa tttctaacac ttttagataa attgaatacc caactttgca
      481 atataacaac agaaacgaaa gaggttaata ataatggaac aaaaaaatga tttttcaaca
      541 attatgactg gtagaagatc cgttcgcgta tatgatgaaa catacaaaat cccacacgaa
      601 gaaatgctgg aaatgatcaa agaagcgacg actgcacctt cttctgtcaa tatgaaacca
      661 tggcgctttg tggtcgctga aagcgaggaa gcaaaagaaa tcttgcgtcc tttgatccgt
      721 ttcaatacga gacaaaatga tacttcttct gcaatggtca tgatttttgg cgatatgcaa
      781 tgttacgaat atggcgaaga aatctacaac caagcttacg aatcagggaa gatgccaaaa
      841 gaagtcagag atcaacaatt agctgcgatc attccttatt acaagagttt ttcaagagaa
      901 gaaatgaacg atgtagtcaa agtcgattct agcttagcgg ctatgcaatt catgttagtt
      961 gcgcgtgatc atggctatga aacaaatcca attggtggat tcgaagcaga tcagttggca
     1021 gaggcatttg gtttggataa agaccgttat gttccagtta ttattttatc aataggaaaa
     1081 gcggtagaag aaggttacga gtctgttcgt ttagcaccag aaaagatcac tatgttcgaa
     1141 taaaaggttt tcagttacta ttcagtagct attgcttttt ttgaatcgtg tgtggtaacc
     1201 taataaaaaa ggaggtcgga aaaatgactg catcgatgaa attacgtttc gaacttttga
     1261 ataacaacta aataaattca aaagacatgt ataaacaaca atttcatgga tggagtatgt
     1321 ccattttttc gaacgacagt agaataggtg aaagctatga cagatttggt gatttagtca
     1381 tagttatttc gttcagagta ggaaaagatg tgatgcagtt tttttgcaaa cagatttttc
     1441 tgctcttttt tagtatcgat aattgttgtt ttacatgaaa taggagtgta aaacatatgg
     1501 aaaatttagc agtaaatata acaaatctac aagtcagctt tggcaaccag ctagaactat
     1561 ctattgattc tcttcgtgtc tatcagcaag atcgtatagg gatcatagga gaaaatggtg
     1621 tcggtaaatc gactttgctc aaactaatag ccggtgaact ttttcccgat catgggaaaa
     1681 tccaaacaga gatcaccttc aactacctgc ctcaattaac ctatctcgct gaagcaaagg
     1741 acctaaattt ggaattagcc agtcatttcc agttaagact ggaagaaact tcagagcgga
     1801 aatggagcgg aggggaagaa cgaaagatcg agttgatacg tcttctttct tcttatgaac
     1861 aagggatgct tctagacgag ccaacaaccc atctagatag aaaaagtatt gatcgactga
     1921 ttgaagagct tcgttattat tatggtacgc tggtttttgt tagtcatgat cgctattttc
     1981 tagatgagct agcatcgaaa atctgggaag taaaagacgg agaaatccga gagttttcgg
     2041 ggaattatag tgcctatctc actcaaaagg aattggagaa aaagactcag ctacgagaag
     2101 cagagtcgat catgaaagag aaaaaacgat tggaaaaatc gatccaagaa aagaaaaaac
     2161 aagcggaaaa gttggaaaaa gtgtccagta aaaagaaaaa gcaacaaatc agactggatc
     2221 ggttgtcttc ctctaaacaa aaagacagtg tacaaaaagc catccaaaag aatgcgaaaa
     2281 cattagagag aagactccaa aaaataggag aaacaaccaa gccgcagcag atgaaacaaa
     2341 tccgttttcc agtaccaaaa tctcttgaac tccacagccg ctatcccatc atgggacaaa
     2401 atgtccaatt ggaaagaagt gggagaacat tactggtaaa tggtgatttt cagttttctt
     2461 taggtaaaaa aatcgcgatt gtcggtgaaa atggttcagg taagacaacc ttattagaac
     2521 atatccgcaa acaaggagaa ggaatccttc tctctccgaa agtaagcttt caagtatatc
     2581 agcaaaaggg ttatcaaatg acatctgaag aatccatcat tcgttttgtc atgagacaaa
     2641 cagagttttc agaatcgctt gtccgtagtt tgctgaatca cttagggttt gctcaggaaa
     2701 ctttgacgaa accgttatgt acattaagtg ggggagaagc gacccgtctg acgattgctt
     2761 tgctttttac taagccaagt aatgtgttgc tgttagatga accgactaat tttattgata
     2821 tggcaacgat cgaagcttta gagaagctaa tgcaaatata tccgggaacg attttgttta
     2881 cttctcatga ttcttacttt gtcgagcgta cggctgatga agtttatgaa ataaaagggc
     2941 agaaaataaa aaaagtactt acgagaaatt tttaaatgtg ctacgatgat tttcgtgaca
     3001 aatttaaagg agtggtacag attgcctaca aataaaaaag aaggaataat ttttacaaca
     3061 atgatgtgtt ttttgatggt cttggggatg agcgcgtata atttatggct gcatcaagat
     3121 cttcatttat caagtttgtt gattggtcta gtgcctggat tcgtggtggc tttcattttg
     3181 gatgtcttca tcatcggtgt gctggcgaaa aaaattgcct ttaaactacc agtaaatcca
     3241 gaaagcaagg tccaaatgat cttaactatt tcatgcttga tggttttagg aatggtgaca
     3301 tgtatgtctt tattcggtgt gatcatggaa caaggaatac ctgatcaatt attctctgct
     3361 tatatgaatg cttggaaaat gaatgtgatt gccgctttac cgttgcagtt gatcatcgtt
     3421 ggcccgcttt caagaagtgt gctaaaagca attcaaagta atgaatgatg gaacactgtt
     3481 atttgaaaaa aggccttggc atgctttttg tcatagtctc ttgaatgcag ttctagaaaa
     3541 atagtgatcc gaacgatagg ttttggcctg tcgttcggat cgctttttct tttccaactg
     3601 tattgctctt ttattgaaga taatttgctc ttcaatagat atttctagaa aggttctgtt
     3661 tatcccttac tcaaaaaact gctgcatttg ttctcttact tgctgacacc attggagttc
     3721 ctgctgacaa agcataattc tgcgctggat cacaacataa cgtccaaatt cttgtttctt
     3781 gtctagcgag aggtcattca attttttttg atttttttgc atgattttgt tcagctgttg
     3841 ttctctagtg aatagcagtc cagtagctgt tgttttatcg agcgtcgaaa aagcatagat
     3901 ttttgctaga aattcatctt tttgcgtagg agcgggagtt tcctctttga tccattcctc
     3961 tacaagagca cgtccttcat cagtcaagtg gtagatcttt ttttgagagt gctcgtcttt
     4021 gtccaaaacg gtcacataac cctgctggtc taacttggca agcgttgtat agatctgact
     4081 gtgatgggct tcccaaaaaa ttgtgatcag ctgcttcaat tcatacccag aacgtggttt
     4141 ggataaaagc acgcataata atatataggc taaagtattc ataaaaatcc tacctctcgt
     4201 cttattttct ttattatatc acagtaaagt ttccacgttt tacatgtgca tattgacatg
     4261 taaaataaat catgtatgat gctaatagag agaaagggat ggaaatattg gagaatcaaa
     4321 tagatataca agaacaaaca aatgaagaaa caaaggggaa aagttcagcg ctagttgtag
     4381 cagctacctg tatgatcgct tttatgggag tgggtctagt agacccaatc ttaaaaacga
     4441 ttgctgcaca gttaaacgct acgccagctg aaacgaccct gttatttact agttacatgt
     4501 tagtcactgg tgtggtgatg ctatttacag gtttcttatc tagtcggatc ggtttgaaaa
     4561 aaacattgat gacaggattg tttatcatcg ttgtattcgc tggtcttggc ggattatctg
     4621 ggaatatcgg aatgctgatt gggttacgtg ccggctgggg tgtcgggaat gctttatttg
     4681 tctctactgc tttagcaacg atcgtcagta ttttagttgg taacacagaa aaagcaatca
     4741 tgatgtatga agcagcactt ggctgcggaa tggctgttgg ccctctagtc ggtggggtct
     4801 tgggcagtgg ttcatggcgt tatccatttt ttggggtagc atttttgatg ttttgcggtt
     4861 ttatagccct agcggtactt ttacctgaaa ctgagaaacc aaagagaaaa gtaggccttt
     4921 gggaaggtgt caaagcgtta agcaatcata aacttcgttc agttggacta atcgcattgt
     4981 tatataattt cggattcttt actttacttg cgtatgcgcc atttttattg attggctatt
     5041 ctgaattaga agtcggtttt gtattttttg gctggggtgt gcttctggct attgcttcga
     5101 ttttcgttgc accggctctt gagcggaagt ttacgactta tcggacaatt ttagcagcat
     5161 ttgtcttatt cattatttgt ttgctgttat taggagctgg aacttctatg ccagtccttg
     5221 taccgatcag cattgtgtta gcaggatttt tccaaggaat tatcaataca ctgttgacga
     5281 cgattgctat ggaaatacca ggtctgcaac gaaacgttgc ttcgtccagc tatagttttg
     5341 tccgcttttt cggtggagct ttagcaccat ttattgcagg tgtgattggt gaacatattg
     5401 gtgaaagcta tgcgttttat tttgcaggtg ccattgttct tatcagtgta tgttgtatca
     5461 tttatcaccg taattatttt gctacaaatg aagaagaata agaaatcaga ggttgtgaaa
     5521 aagctgtcct gcatcaagtc ataagaa
//
LOCUS       DAZKOU010000299         5132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.guided.131, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000299 DAZKOU010000000
VERSION     DAZKOU010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.guided.131"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1270
                     /locus_tag="ACG827_003086"
     CDS             <1..1270
                     /locus_tag="ACG827_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0485110.1"
                     /translation="PAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPN
                     GKISMVSFREIIEAQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSV
                     AINGIITSILMRAKPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVV
                     QEMEERYEKFAATGVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASN
                     EVEDAIIRLAQMARAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTII
                     DSNGAEKLLGRGDMLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMP
                     TDEVETTGAPEQPQDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHG
                     VVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            1500..2567
                     /gene="rlmN"
                     /locus_tag="ACG827_003087"
     CDS             1500..2567
                     /gene="rlmN"
                     /locus_tag="ACG827_003087"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0485111.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            2557..2763
                     /locus_tag="ACG827_003088"
     CDS             2557..2763
                     /locus_tag="ACG827_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485112.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(2760..3863)
                     /locus_tag="ACG827_003089"
     CDS             complement(2760..3863)
                     /locus_tag="ACG827_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0485113.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            4416..4964
                     /gene="aac(6')"
                     /locus_tag="ACG827_003090"
     CDS             4416..4964
                     /gene="aac(6')"
                     /locus_tag="ACG827_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0485114.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1689 a          885 c         1091 g         1467 t
ORIGIN      
        1 gccggctgta ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc
       61 tttagcggcg aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat
      121 tgaagtacca aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc
      181 aaaccaccca gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca
      241 aacggctgat ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa
      301 gtcagtagca attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt
      361 aaaactgatg atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca
      421 tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt
      481 acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg
      541 ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc
      601 attcatcgta gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt
      661 tgaagatgcg attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct
      721 agctactcaa cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc
      781 cagaatggca tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg
      841 ggcagaaaag ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc
      901 gatccgtgtg caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt
      961 tacagatcag caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac
     1021 gacaggtgct ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt
     1081 tgaaatgcaa acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg
     1141 agcagcacgt ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag
     1201 caagcctcgg aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa
     1261 tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg
     1321 catgatgata ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc
     1381 ttgtcttttt agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat
     1441 taaggaaatg aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt
     1501 tggaaaaaca gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa
     1561 acggcgagaa aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa
     1621 gtaatttcac agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt
     1681 ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat
     1741 atttgtttga actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg
     1801 gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa
     1861 gcgggctgct caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga
     1921 tggtccaaca ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa
     1981 tggggatcgg tgagccattt gataattacg acaatgtaat ggactttttg catatcatca
     2041 atgatgccaa aggattggca attggtgctc ggcacattac agtttccact agtggattag
     2101 cacacaaaat caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc
     2161 acgcgccgaa taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg
     2221 agaaattgat ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg
     2281 aatatatcat gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc
     2341 tattaaaaga taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg
     2401 aacatgatca atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga
     2461 agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt
     2521 gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc
     2581 gttttttcaa ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat
     2641 aagagaaaag attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc
     2701 aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt
     2761 taactgattt tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca
     2821 agaatggcct gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt
     2881 gttgccatgg ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg
     2941 gttaacgata ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg
     3001 aactgctgat ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg
     3061 tatccctcca ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa
     3121 ttatagttat tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg
     3181 acttgttcaa ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca
     3241 taagtcaaga gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag
     3301 cctagatcat cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca
     3361 atacctgccc ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg
     3421 tgtacaggtg gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg
     3481 acacataccg cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt
     3541 ttagctagtt tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca
     3601 aacaggacgt ctgctttttg gacactttca tgctggatca actgttcaat atttccataa
     3661 gttgaattac ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact
     3721 tttattttgt tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc
     3781 ccatattctt tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt
     3841 ggtaagaaaa cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa
     3901 ttttaaagag tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag
     3961 gataagtgat gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac
     4021 gaacaagtac aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct
     4081 accgattccc tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat
     4141 gaaaacaggt tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat
     4201 atcatgatga acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt
     4261 ttgaataaag tgtttctgta taattttttt attttataag taagataaat aatgaaagaa
     4321 tagagatact gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg
     4381 actgttagaa taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta
     4441 ataatccagt attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat
     4501 atggagacag ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag
     4561 cagcggtaga ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca
     4621 caggttggga attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta
     4681 ctcgattagt caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt
     4741 taggtacgga tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata
     4801 catttgataa agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa
     4861 aattaggtta taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata
     4921 tttggatggc aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga
     4981 cagaagtaat cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt
     5041 gtgaaattcc aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt
     5101 gaaaaagtcg cttagtttcg agtagtaaga aa
//
LOCUS       DAZKOU010000300         4802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.guided.136, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000300 DAZKOU010000000
VERSION     DAZKOU010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.guided.136"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG827_003091"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG827_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0485115.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="ACG827_003092"
     CDS             763..1917
                     /locus_tag="ACG827_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0485116.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="ACG827_003093"
     CDS             2132..3100
                     /locus_tag="ACG827_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0485117.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="ACG827_003094"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="ACG827_003094"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0485118.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="ACG827_003095"
     CDS             4130..4738
                     /locus_tag="ACG827_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0485119.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1281 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DAZKOU010000301         4620 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.guided.141, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000301 DAZKOU010000000
VERSION     DAZKOU010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.guided.141"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..607
                     /gene="dfrG"
                     /locus_tag="ACG827_003096"
     CDS             110..607
                     /gene="dfrG"
                     /locus_tag="ACG827_003096"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0485120.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(679..2631)
                     /locus_tag="ACG827_003097"
     CDS             complement(679..2631)
                     /locus_tag="ACG827_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0485121.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2638..2874)
                     /locus_tag="ACG827_003098"
     CDS             complement(2638..2874)
                     /locus_tag="ACG827_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485122.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3276..3566
                     /locus_tag="ACG827_003099"
     CDS             3276..3566
                     /locus_tag="ACG827_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0485123.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(3838..4187)
                     /locus_tag="ACG827_003100"
                     /pseudo
     CDS             complement(3838..4187)
                     /locus_tag="ACG827_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4280..4582
                     /locus_tag="ACG827_003101"
                     /pseudo
     CDS             4280..4582
                     /locus_tag="ACG827_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
BASE COUNT         1466 a          759 c          808 g         1587 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcat aatgagaaaa ggttttttga
       61 tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa tgaaagtttc
      121 tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca ttccttggag
      181 gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga taatattagg
      241 taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata ttattctgac
      301 gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag aagatgtttt
      361 tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga tttataattt
      421 gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat tcgaaggaga
      481 tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc aaaaagggat
      541 aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa aaaacttatt
      601 gagttaacgg gtgcgttaat tgaagaagaa cagtaaagat aaagcacaag agcttgggat
      661 tactccaagc tctttttgtc atttgaaata aattatatct ttccaatcaa attgtttatc
      721 tgtaatccct aatctttgag caggtgtcaa aaccttttta tccgctgatt taaaagggcg
      781 acaaaagttg tagtaagttc ttagaattgt aatcgcaaat tgagcatact tcggattgaa
      841 gttagcgtaa atatagctct tcttatcacc acgagctgtt gttaatggtc tttcaagaga
      901 tgatatataa cgtcttatct gttgaataaa gctattggtc gaatggtcat taacatttaa
      961 tagcatcttt gcgatttcgt ttggttccag agcagataaa tccgttctac aatcaactga
     1021 ataaaatcct ttatccttag tggctaatgg atgtttgata gggttatcgg catatttgcg
     1081 atatctttct ccatctttac taatagcctc ttcgtggaat tgatgacttg ttagtaattc
     1141 agttagataa tgagatgcca aagtccgtag aaactttgtt ttgaatcctc tgatatctcc
     1201 ccaatcgagc agttctgctt ttgcttgtcc gaattctttt aagcattgtt ttctgctttt
     1261 cgtcttgtta acttggcaaa taaaatgatg tgcatcgctc aaccttaatt ctttcgtgaa
     1321 tactctataa aaggcgtttc taatagagaa gtcatcatca ctaatcattc gccattcgtc
     1381 ggcattaacc atttgtttaa ttaaccaata gtgagcagtt gtagtataag gagcatcaac
     1441 atttaaacca tcgacaaatc tactcctatt agtaattttc cctaattcgt gaagataagc
     1501 attcctattt tcgctatcgt tagcggatgg ttcttgtgga taataagacc aatctaacct
     1561 atcattttta cgactaaaag tattgagatg gtcttctttg aattttcttg tatcttcatt
     1621 taattcatcc attgaaatat tccaatcata ggcaacatca gaacggaaca catatcttga
     1681 taagacctca gcagatacaa ctatatacgt ttgcatatta aggtcttcaa accccgtata
     1741 tttctttgaa ccttgtcctt tcttacggac gttatttagg tagtagtgca tcttgtccgt
     1801 attaagccac atctcgttaa atttctttgt ttgaaggggt tgtgtctcat atcgttctaa
     1861 aaactccaaa caacgcctat acagccactc cagcttatga tagtaggttc caactccaat
     1921 tcctaaaata tcacaagtac gactgactga aacccttcca acaaccatct tagcgaacat
     1981 tggaagaatg gtgttctttt gttggtgata agtagtggtt tcttctcttt ttggtagaac
     2041 attagtgaac ttcttacacg ccttacattg atagcgttga gatttgcccc tacttttccc
     2101 ccttttgtaa aattgcttcg gctcattgaa tggggttgat tcttcttccg agcagccttc
     2161 tttatgaaat tgataatctg gctcaacatc ttggatgcta ttgatttcta taaggcgttt
     2221 aatttcttca acaacagacc aattagataa aggggttact gtacagtttt gcgatacacc
     2281 tctgccaata ggattgtcat tgcagtacat acctttatga cctttatctt tggaactacc
     2341 agtcatttta tatcgacttg gtttcccttt tacatctttg aatttgtgct gttctttccc
     2401 aaaattacaa caaaacggat tgatgcagta attatattga atgggatatt cagaaccgtt
     2461 ccatttaaaa gacgttggca agaacaacat tttgttatac cttgttgcaa atcgttttgg
     2521 agttaataaa tcatattgtt tagccctatc ttttaattct tcgtttgaaa tcggaatttt
     2581 aacatctaca atttcatctt ccttcgtggc taatctttta agcttggtca ttgtctatca
     2641 acccactcta tctttgatac ccattttttc aacaatccgc ttattacttc gcttgttggc
     2701 aatccattca ataaacttct tatcgtctaa aatgttcaat aagaattcat ccactgcttc
     2761 gctttcagta cgctctgcat attcagcgta ttgcttaact atcaccctta cttgctctga
     2821 tatttcccaa tccacagact tagcgttctt attttttggc tcaagaaatt gcataggaaa
     2881 atgcacccct ttttgcttag taaattgtaa ccattttaac acaccaattt gcattcctac
     2941 aataattatt tcacgttcgc atttttatat acattttaac ttacattttg tggtgtaaat
     3001 taacaacaaa cgttgattta acaataaaaa acgagtatca aattcagttg caatttgata
     3061 ctcatttagg tttacatttt cactttttgt actccaaaac tgaactactt aaatctatac
     3121 tgctgcctct tttttttagt acatgtcctt catcttcgca ggaagtgcca atatgatttt
     3181 aagaaaggta atagacaaaa agtcaaaaag ataaaacaga gtttcttctt tacaaaggga
     3241 atgtatgttc gtataatggt attgaggtga aggttatgga gaaaaaatat ggaacagtag
     3301 ataagatccg tactttgcaa gttagtgcca gaccgttgat ccgtttcagt ttaaatgggg
     3361 tcaattgttt gattgcttcc catagtttga acttcctagc tgaagtagac gaagggatga
     3421 agttagtcgt tacaggttat tataataata gaaagcaatt tgttgtgaga tcctatcatg
     3481 tgttagggaa acccaaaatt gtggtagaat atgagaaaag tctataccca agaaaaaagg
     3541 tccgacaaga tgaaagaata aactagaaaa caagcggctg agacaaaaat gtcctagccg
     3601 cttgttttgg aaagatggat gcagacgatg ggatttgaac ccacacgagc atacgctcac
     3661 aagaacctca atctatatat aactacatgt atttaagtat ttgtgatgaa tcactaaact
     3721 ttccaaactc atttgaggga tattgcttaa agattgaaca gatcaattcg aagccctatt
     3781 agtattcgct caatgggggg atcagaggcc ataaaaagag actagtcatt actagtctca
     3841 ggtagtggaa ttgtaggatt ttaaacgttc ctggagagta ccggtatgta actcgaagag
     3901 gtgattatca tagtcgtaaa agtaaattga agaagcctcg cctttcacac gagaacgtcc
     3961 tggttcgaca gttaaaccta acatttggat ttttgagaca aacgaatcta tatcttgttc
     4021 gtcaatttta aaagcaatat gattgtatgt ctttggcagt ttattcgacg aattctgcat
     4081 tactgcaatc cataggtcct taactaagaa aaatttttct ggatataatg aaaatttttt
     4141 tgttgactat cgtagatttc ttttgcatca aataaggtat ttaacaatta agtatttaaa
     4201 agataacttt taagttatct ttaagtaatg cttaaagcta tgttatactc tattaggaaa
     4261 tataagaagg agtataaaaa tgaacaaaca taatagaaaa ataacgaata acaaagaact
     4321 gatcttgtta ctaataggga tttggggtat ctttttactc tttaatatgt atatgccgac
     4381 tatgagagcg gatgacgtag tttatgcaag tagattagat gaacttggct atttgggtgc
     4441 ttcgatagaa cattataaga catggagttc aagaattatt atagaactat tcttaatgtt
     4501 cttttctaaa cactttatgc tatggaaatt attaaattct acgattatgt taggaattgt
     4561 agtattactt tgcaagtatg ttgagagtat aaaatatttt gtgtaaatga aaaaatccat
//
LOCUS       DAZKOU010000302         3278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.guided.161, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000302 DAZKOU010000000
VERSION     DAZKOU010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.guided.161"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..188
                     /locus_tag="ACG827_003102"
     CDS             3..188
                     /locus_tag="ACG827_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0485124.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="ACG827_003103"
     CDS             203..1072
                     /locus_tag="ACG827_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0485125.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="ACG827_003104"
     CDS             1053..1787
                     /locus_tag="ACG827_003104"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0485126.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="ACG827_003105"
     CDS             1820..2728
                     /locus_tag="ACG827_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0485127.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="ACG827_003106"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="ACG827_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAZKOU010000303         1270 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.guided.213, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000303 DAZKOU010000000
VERSION     DAZKOU010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.guided.213"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..1170
                     /locus_tag="ACG827_003107"
     CDS             259..1170
                     /locus_tag="ACG827_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0485128.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          454 a          185 c          258 g          373 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattc aaggaggtaa ggatggcgga atgaaaccaa cgaaattaat
     1201 gaacagcatt attgtactag cacttttggg gtaacgttag ctttttaatt taaaacccac
     1261 gttaactagg
//
LOCUS       DAZKOU010000304          969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3400
            SAMEA115706690-rid22579803.guided.227, whole genome shotgun
            sequence.
ACCESSION   DAZKOU010000304 DAZKOU010000000
VERSION     DAZKOU010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706690
            Sequence Read Archive: ERR13196425
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287736.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287736.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:07:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,109
            CDSs (total)                      :: 3,050
            Genes (coding)                    :: 2,953
            CDSs (with protein)               :: 2,953
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 42 of 97
            Pseudo Genes (incomplete)         :: 50 of 97
            Pseudo Genes (internal stop)      :: 22 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706690-rid22579803.guided.227"
                     /isolate="VRE3400"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..75
                     /locus_tag="ACG827_003108"
     CDS             25..75
                     /locus_tag="ACG827_003108"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0485129.1"
                     /translation="MWTLSKQTKNSDYADK"
     gene            200..937
                     /gene="erm(B)"
                     /locus_tag="ACG827_003109"
     CDS             200..937
                     /gene="erm(B)"
                     /locus_tag="ACG827_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0485130.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          362 a          165 c          144 g          298 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcga aacagactaa aaacagtgat
       61 tacgcagata aataaatacg ttagattaat tcctaccagt gactaatctt atgacttttt
      121 aaacagataa ctaaaattac aaacaaatcg tttaacttct gtatttgttt atagatgtaa
      181 tcacttcagg agagattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag
      241 tgaaaaagta ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta
      301 cgaaattgga acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt
      361 aacgtctatt gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa
      421 tactcgtgtc actttaattc accaagatat tctacagttt caattcccta acaaacagag
      481 gtataaaatt gttgggaata ttccttacca tttaagcaca caaattatta aaaaagtggt
      541 ttttgaaagc cgtgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac
      601 cttggatatt caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt
      661 gcttaagctg ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa
      721 acttacccgc cataccacag atgttccaga taaatattgg aagctatata cgtactttgt
      781 ttcaaaatgg gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc
      841 aatgaaacac gccaaagtaa acaatttaag taccattact tatgagcaag tattgtctat
      901 ttttaatagt tatctattat ttaacgggag gaaataattc tatgagtcgc ttttttaaat
      961 ttggaaagt
//
